Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of Rasna panchaka (indigenous drug) as oxidative stress down-regulator using serum-free explant culture system

CONTEXT: The importance of phytochemicals/natural products as potential therapeutic agents in the present context is gaining a lot of importance. India with a rich heritage of such preparations needs evaluation as potent drugs. Explant culture system is a method, which is sensitive, reliable, reproducible and is capable of mimicking the in situ conditions maintaining the tissue in sufficiently high level of integration. AIM: The current study aimed to test the antioxidant activity of test compounds, namely, traditional aqueous (4212) and aqueous-methanolic (4308) extracts of Rasna panchaka using liver explant cultures. MATERIALS AND METHODS: Dose-response optima of extracts (0.2–10 μg/mL) were determined using mouse liver explant culture system up to 48 h. The antioxidant property of extracts was assessed by primary oxidative defense parameters, namely, superoxide-dismutase (SOD), catalase, reduced glutathione (GSH), and malondialdehyde (MDA). RESULTS: The results indicated that the cellular architecture of the cultured tissue was well conserved in the first 6 h with a gradual display of specific changes in the next 24 h. There was a significant increase in MDA levels in experimental groups indicating the oxidative stress induction in explants. A dose of 2.0 μg/mL extracts have shown statistically significant (P < 0.05) protection against oxidative stress. MDA levels, a measure of lipid peroxidation, were significantly (P < 0.01) reduced by 50% in extract treated explants compared to control. This effect was accompanied by the increase in the first defense enzymes SOD (50%) and catalase (18%) with no change in reduced GSH levels. CONCLUSION: The study enforces the importance of “explant culture system,” as it not only reduces the use of nonclinical/animal model but also is rapid and sensitive. Further, results of the current study also suggest that aqueous-methanolic extract of Rasna panchaka is having superior antioxidant activity compared to traditional water extract.
SENSITIVITY ANALYSIS OF WATER FLOODING OPTIMIZATION BY DYNAMIC OPTIMIZATION

This study concerns the scope to improve water flooding in heterogeneous reservoirs. We used an existing, in-house developed, optimization program consisting of a reservoir simulator in combination with an adjoint-based optimal control algorithm. In particular we aimed to examine the scope for optimization in a two-dimensional horizontal reservoir containing a single high permeable streak, as a function of reservoir and fluid parameters, which we combined in the form of 10 dimensionless parameters. We defined the parameter NPVimprovement to indicate the improvement in net present value (NPV) that can be achieved through optimization. For initial screening of the effect of the dimensionless parameters, a two-level D-optimal design of experiments (DOE) technique was used to obtain a linear response surface model with the aid of 11 water- flooding simulations. As a result 8 dimensionless groups were selected for more detailed analysis, and a full quadratic NPVimprovement model was constructed using a three-level D-optimal design using 50 simulations. It should be reminded that all the D-optimal matrix designs were generated by using commands of statistics Toolbox of MATLAB software. Finally, Pareto charts were plotted to visualize the sensitivity of the model as a function of the dimensionless parameters. Based on the present model we can draw the conclusion that the parameters L Ls / (relative streak length), streak k k max max / (relative streak permeability) and the ratio of water cost and oil price have the
Stimulation Modeling on Three-Dimensional Anisotropic Diffusion of MRI Tracer in the Brain Interstitial Space

Purpose: To build a mathematical model based magnetic resonance (MR) method to simulate drug anisotropic distribution in vivo in the interstitial space (ISS) of the brain. Materials and Methods: An injection of signal intensity-related gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA), which is an exogenous drug, was administered, and its diffusion was traced in the ISS of the brain using MRI. Dynamic MRI scans were performed to monitor and record the changes in signal intensity in each pixel of the region of interest. The transport parameters were calculated using the modified equation to simulate three-dimensional anisotropic diffusion, which was resolved using a Laplace transform and a linear regressive model. Results: After Gd-DTPA was introduced into the caudate nucleus, its distribution was demonstrated in real time. As the Gd-DTPA gradually cleared, the associated hyperintensity attenuated over time. The average diffusion coefficient (D) and the clearance rate constant (k) were (1.305 ± 0.364) × 10−4 mm2/s and (1.40 ± 0.206) × 10−5 s−1, respectively. Discussion: The combination of trace-based MRI and modified diffusion mathematical models can visualize and measure the three-dimensional anisotropic distribution of drugs in the ISS of the brain.
Efficiency and Prospects of Diagnosis of the Functional State of the Dental System

This paper presents the results of studying the effectiveness of the developed method for diagnosing the functional state of the dental system. The results indicate that the accuracy of the method is about 80%. The results of previous studies are reviewed and the prospects for diagnosis of the functional state of the dental system as a research and practical trend in dentistry are outlined. The physiological regularities of the dental system functioning and relationship between its functional status and the autonomic nervous and postural systems prompt a new trend of research, functional diagnosis in dentistry and interpretation of the findings of functional studies. The prospects of functional diagnosis in dentistry are discussed.
Handling Stress as a Measurement of Personality in Great Tit Nestlings (Parus major)

Interest in personality is growing in a wide range of disciplines, but only in a few systems it is possible to assess the survival value of personality. Field studies looking at the relationship between personality and survival value early in life are greatly hampered by the fact that personality can at present only be assessed after individuals become independent from their parents. In passerines, for example, this is often after a period of intensive selection for the survival on fledglings. The main aim of this study is therefore to develop a method to measure personality before this period of selection. For this purpose, we developed the handling stress (HS) test. We measured HS in 14-d-old great tit nestlings by counting the number of breast movements (breath rate) in four subsequent 15-s bouts for 1 min; before and after they were socially isolated from their siblings for 15 min. To calculate the repeatability of HS, we repeated the test 6 mo later. To assess the relationship between HS and exploratory behaviour, we correlated the outcome of both tests. We ran tests both on birds of lines selected for extreme personality and on wild birds from a natural population. We found that birds selected for fast exploration reacted more to HS compared with birds selected for slow exploration and that HS was repeatable in different life phases. We confirmed this by finding an increase in the HS with increasing exploratory scores in wild birds. These results show that we can use the HS test as a measurement of personality, making it a potential tool for studying the relationship between personality and survival value early in life.
DNA carrying nucleotide sequence information from two different parent DNA

Title : RNA interference in gene cloning/expression 2 3 Vikash Bhardwaj 4 Molecular Biology and Genetic Engineering Domain, Lovely Professional University, Punjab, India 5 144411. 6 Email : vikashbhardwaj@gmail.com 7 8 Abstract 9 DNA cloning is a simple, straightforward process. It has been put into practice as a 10 standard lab procedure for cloning the gene of interest for specific protein expression 11 and other molecular or cellular studies. The present study is the first of its kind to 12 hypothesize the formation of interfering recombinant plasmid during a DNA cloning 13 reaction that is believed to interfere with the protein or gene expression. This might 14 lead to a false conclusion in a cellular study and also may lower the protein 15 expression during the production stage. 16 17
SPEECH RECOGNITION : CONTEXTUAL ACOUSTIC WORD EMBEDDINGS

End-to-end acoustic-to-word speech recognition models have recently gained popularity because they are easy to train, scale well to large amounts of training data, and do not require a lexicon. In addition, word models may also be easier to integrate with downstream tasks such as spoken language understanding, because inference (search) is much simplified compared to phoneme, character or any other sort of sub-word units. In this paper, we describe methods to construct contextual acoustic word embeddings directly from a supervised sequence-to-sequence acoustic-to-word speech recognition model using the learned attention distribution. On a suite of 16 standard sentence evaluation tasks, our embeddings show competitive performance against a word2vec model trained on the speech transcriptions. In addition, we evaluate these embeddings on a spoken language understanding task, and observe that our embeddings match the performance of text-based embeddings in a pipeline of first performing speech recognition and then constructing word embeddings from transcriptions.
The role of myofascial massage in spinal pains in professionally active women

Background: Pain ailments of the spine are one of the most common diseases of the system of bones and joints. 75% to 85% of the world’s population suffer or will suffer from it, and lumbar pain is the most common cause of disability of persons over 45 years of age. They are usually the beginning of a chronic, often progressing ailments often leading to disability, incapacity to work and restrictions in daily life. Myofascial massage helps reduce pain sensations. Is a specific method of gradual stretching soft tissues and making muscle fibres more elastic. It reduces increased muscle tone, muscle insertions and tendons. Material/Methods: The study involved 30 women with a mean age of 52.7 years. The study relied on performing 10 myofascial massages of the spine within 2 weeks. Results: The statistical analysis of the Wilcoxon’s signed-ranked test showed statistically significant results for tested variables of the Laitinen scale (the intensity of pain before and after the treatment at p = 0.00, the incidence of pain before and after the treatment at p = 0.00, the use of analgesics before and after the treatment at p = 0.00, limitation of physical activity before and after the treatment at p = 0.00). Conclusions: The applied therapy involving a series of myofascial massages supporting the structures of the motor segment, improving the elasticity of the massaged tissues and normalising the muscle tone resulted in reducing pain ailments in the lumbo-sacral section of the spine. Improvement in the patients’ well-being and increased desire for physical activity took place.
Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate

The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to design a probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia. Key words : probe, env-tm, JDV, specifity, sensitivity.
Longitudinal data exploration of upper respiratory tract infection disease in Bandung city

Longitudinal data have a complex structure. Unlike cross-sectional data, there are more associated features with longitudinal data. Associated features to longitudinal data including the heterogeneity between subjects, the serial correlation and the characteristics of changes over time. Therefore exploring longitudinal data is more complicated than that of cross-sectional data. Various methods were developed by many researchers to explore longitudinal data. In general, there are at least three things that could be described from longitudinal data: mean structure, variance structure, and correlation structure. Visual methods and subject-specific profile were studied for such datasets to detect these structures. Methods were applied to data sets from upper respiratory tract infection data in Bandung City. From the result, we can describe the growth patterns at both group and individual level and then selecting suitable statistical models which contain within and between groups variations.
Voluntary Intake and Digestibility of Fresh, Wilted and Dry Leucaena (Leucaena leucocephala) at Four Levels to a Basal Diet of Guinea Grass (Panicum maximum)

Sixty crossbred Anglo-Nubian goats (growing females), 18-24 mo of age, with a mean pre-experimental live weight of 20.9±0.44 kg were used to investigate voluntary dry matter intake (DMI) and digestibility of supplements of Leucaena leucocephala (LL) leaves to a basal diet of guinea grass. The experiment was a 3 forms of presentation: fresh, wilted and dry×4 levels of supplementation: 10, 20, 30 and 40% of total forage allowance with LL leaves. The goats were assigned based on weight and age to one of the twelve diets in a randomized manner and each level had five animals (replicates) per diet for 56 days. The form of presentation of LL leaves had effects on dry matter (DM) and nutrient composition. DM and NDF were higher in the dry LL leaves (p<0.05). In the diets, CP increased as the level of supplementation with LL leaves increased (p<0.05). Mean CP was similar in the fresh (14.8±3.5%), wilted (14.3±3.3%) and dry forms (13.9±3.1%). Neutral detergent fibre (NDF) decreased linearly (p<0.05) with increase in levels of supplementation. Organic matter (OM) followed the trend of CP concentration (p<0.05). Drying was observed to reduce the availability of CP at the different levels of supplementation and subsequently this affected the NDF of the diets. DM intake of the goats on the fresh and wilted LL leaves was significantly higher (p<0.05) than in those on the dry LL leaves. DM intake of the basal diet was observed to decrease as the level of supplementation (p<0.05) increased regardless of the form of presentation of LL. Growth rate was best (p<0.05) on the fresh form followed by wilted and the least in the dry form. Growth rate decreased linearly (p<0.05) with increase in the level of supplementation in the dry form. The form of presentation and level of supplementation influenced DM, CP and OM digestibility (p<0.05). Except for NDF, the digestibility of DM, CP and OM were better (p<0.05) in the goats on fresh LL leaves compared to the wilted and dry leaves. Based on the data on DMI, growth rate and apparent nutrient digestibility coefficients, the results suggest that LL leaves is best utilized when fed fresh or wilted to goats. In the dry form of presentation, the best level of supplementation without a reduction in voluntary DMI and growth rate is the 20%. In the fresh and wilted form, LL leaves could be fed up to the 40% level with improved DMI, growth rate and nutrient utilization. (Asian-Aust. J. Anim. Sci. 2002. Vol 15, No. 8 : 1139-1146)
Effectiveness of exercise in reducing cerebral stroke risk factors: A systematic review and meta-analysis

Objectives: This study aimed to explore the relationship between exercise and cerebral stroke and provide evidence for the prevention of cerebral stroke. Materials/Methods: All clinical trials of exercise intervention for atherosclerosis were systematically reviewed. Five major databases were searched to retrieve relevant studies from their inception to May 2022. According to the magnitude of heterogeneity, the random and fixed-effect models were used to test reasonably. Results: According to the inclusion and exclusion criteria, 1341 articles were screened and 13 articles involving 825 patients were identified. The result showed that in the randomized controlled trials carotid intima-media thickness index was lower in the exercise group (−0.04 mm, 95% confidence interval: −0.07 to −0.01). All were statistically significant (P < .005) and subgroup analysis showed that the intervention period and paper quality are sources of heterogeneity. Conclusions: The results of this systematic review suggest that exercise is associated with a slow increase in carotid intima-media thickness, which may provide evidence that exercise helps reduce cerebral stroke.
On the substructure of the cosmological constant

We summarize the findings of our paper $\tt \small arXiv:1701.08776$ $\tt \small[hep-th]$. We start by defining the quantum break-time. Once one understands a classical solution as expectation value of an underlying quantum state, it emerges as time-scale after which the true quantum evolution departs from the classical mean field evolution. We apply this idea to de Sitter space. Following earlier work, we construct a simple model of a spin-2 field, which for some time reproduces the de Sitter metric and simultaneously allows for its well-defined representation as coherent quantum state of gravitons. The mean occupation number $N$ of background gravitons turns out to be equal to the de Sitter horizon area in Planck units, while their frequency is given by the de Sitter Hubble parameter. In the semi-classical limit, we show that the model reproduces all semi-classical calculations in de Sitter, such as thermal Gibbons-Hawking radiation, all in the language of quantum $S$-matrix scatterings and decays of coherent state gravitons. Most importantly, this framework allows to capture the $1/N$-effects of back reaction to which the usual semi-classical treatment is blind. They violate the de Sitter symmetry and lead to a finite quantum break-time of the de Sitter state equal to the de Sitter radius times $N$. We also point out that the quantum-break time is inversely proportional to the number of particle species in the theory. Thus, the quantum break-time imposes the following consistency condition: Older and species-richer universes must have smaller cosmological constants. For the maximal, phenomenologically acceptable number of species, the observed cosmological constant would saturate this bound if our Universe were $10^{100}$ years old in its entire classical history.
Gecko: Hierarchical Distributed View of Heterogeneous Shared Memory Architectures

The November 2018 TOP500 report shows that 86 systems in the list are heterogeneous systems configured with accelerators and co-processors, of which 60 use NVIDIA GPUs, 21 use Intel Xeon Phi cards, one uses AMD FirePro GPUs, one uses PEZY technology, and three systems use a combination of NVIDIA GPUs and Intel Xeon Phi co-processors. From a software standpoint, managing data locality on such heterogeneous systems is as important as exploiting parallelism in order to achieve the best performance. With the advent of novel memory technologies, such as non-volatile memory (NVM) and 3D-stacked memory, there is an urgent need for effective mechanisms within programming models to create an easy-to-use interface that addresses such memory hierarchies. It is also equally crucial for applications to evolve with data locality for the expression of information. In this paper, we propose Gecko, a novel programming model that addresses the underlying memory hierarchy topology within computing elements in current and future platforms. Gecko's directives distribute data and computation among devices of different types in a system. We develop a source-to-source transformation and a runtime library to efficiently manage devices of different types to run asynchronously. Although our current implementation of Gecko targets Intel Xeon CPUs and NVIDIA GPUs, it is not restricted to such architectures. We used SHOC and Rodinia benchmark suites to evaluate Gecko. Our experiments used a single node consisting of four NVIDIA Volta V100 GPUs. Results demonstrated scalability through the multi-GPU environment. We observed 3.3 times speedup when using multiple GPUs.
Illness Perception of Anxiety Patients in Primary Care in Singapore

Background: The majority of people with anxiety tend to seek help in primary care. Patients' illness perception regarding their own anxiety can influence the assessment, treatment processes, and outcomes. This cross-sectional study explored possible relationships between patients' illness perception of their anxiety and the severity of their anxiety. Materials and Methods: Ninety-five patients with anxiety were recruited at two primary care clinics in Singapore. Their responses to the generalized anxiety disorder-7 (GAD-7) and illness perception questionnaire mental health (IPQ-MH) were examined with Spearman's rho correlation coefficients and multiple regression analyses. Results: Four illness perception subscales, i.e., consequences (rs = 0.23), personal control (rs= –0.27), coherence (rs= –0.22), and biological (rs = 0.34) significantly correlated to anxiety (P < 0.05). A multiple regression analysis identified that attribution to biological factors (β = 0.348, P =0.001) and attribution to personal control (β = -0.262, P =0.008) were significantly associated with anxiety. Conclusions: Interventions for anxiety reduction in primary care can be enhanced with methods that promote (1) patients' awareness of the reasons for their anxiety beyond mostly bodily ones to include psychosocial ones and (2) patients' confidence in their own capacity to influence their recovery.
A Pilot Study on Circadian Activity Rhythm in Pediatric Attention-Deficit Hyperactivity Disorder

A recent study has applied a novel statistical framework (functional linear modeling: FLM) to the study of circadian activity rhythm (CAR) in adult attention-deficit hyperactivity disorder (ADHD), pointing out the absence of the physiological post-lunch dip. The aim of the present study was to apply FLM to explore the features of CAR in pediatric ADHD. To this end, a secondary analysis of previously collected data was carried out. Twenty-four ADHD children (four females, mean age 8.67 ± 1.74) and 107 controls (C, 60 females, mean age 10.25 ± 0.48) were examined. The actigraph model Actiwatch AW64 was used to objectively monitor sleep/wake behavior and CAR. In the original study each participant wore the actigraph on the non-dominant wrist for one week. FLM was applied to examine the differences between groups in CAR. Compared with C, the CAR of ADHD children was distinguished by a higher motor activity during the whole of the daytime and within a reduced time window during the nighttime.