Miracle-dz/UniFAR-FTUs huggingface dataset:
AENDO June 41/6

Zhou, Min, Gino Vallega, Konstantin V. Kandror, and Paul F. Pilch. Insulin-mediated translocation of GLUT-4containing vesicles is preserved in denervated muscles. Am J Physiol Endocrinol Metab 278: E1019–E1026, 2000. — Skeletal muscle denervation decreases insulin-sensitive glucose uptake into this tissue as a result of marked GLUT-4 protein downregulation (,20% of controls). The process of insulin-stimulated glucose transport in muscle requires the movement or translocation of intracellular GLUT-4-rich vesicles to the cell surface, and it is accompanied by the translocation of several additional vesicular cargo proteins. Thus examining GLUT-4 translocation in muscles from denervated animals allows us to determine whether the loss of a major cargo protein, GLUT-4, affects the insulin-dependent behavior of the remaining cargo proteins. We find no difference, control vs. denervated, in the insulin-dependent translocation of the insulin-responsive aminopeptidase (IRAP) and the receptors for transferrin and insulin-like growth factor II/mannose 6-phosphate, proteins that completely (IRAP) or partially co-localize with GLUT-4. We conclude that 1) denervation of skeletal muscle does not block the specific branch of insulin signaling pathway that connects receptor proximal events to intracellular GLUT-4-vesicles, and 2) normal levels of GLUT-4 protein are not necessary for the structural organization and insulin-sensitive translocation of its cognate intracellular compartment. Muscle denervation also causes a twofold increase in GLUT-1. In normal muscle, all GLUT-1 is present at the cell surface, but in denervated muscle a significant fraction (25.1 6 6.1%) of this transporter is found in intracellular vesicles that have the same sedimentation coefficient as GLUT-4-containing vesicles but can be separated from the latter by immunoadsorption. These GLUT1-containing vesicles respond to insulin and translocate to the cell surface. Thus the formation of insulin-sensitive GLUT-1containing vesicles in denervated muscle may be a compensatory mechanism for the decreased level of GLUT-4.
Deep Reinforcement Learning for Action Based Object Tracking in Video Sequences

In this paper, we propose a valuable route for visual object tracker which catches a bounding box to zone of premium physically in the video frames by recognizing the activity got the hang of utilizing the convolution neural systems. The proposed convolution neural network used to control tracking actions is done with various training video sequences and fine-tuned during the actual tracking of the object. Pretrain of the video is done using deep reinforcement learning (RL) along with the supervised learning. Mostly named information from the RL can be utilized for semi supervised learning and assessing through object tracking benchmark dataset, the proposed tracker is confirmed to accomplish a good performance. The proposed method, which operates in real time on without graphics processing unit, outperforms the state of real time trackers with proper accuracy with performance 10%.
NOTES AND CORRESPONDENCE On the Effective Solar Pathlength

The effects of atmospheric spherical curvature and refraction and their impact on radiative transfer have been studied. It is shown that formulas employed in GCMs for atmospheric curvature and refraction underestimate the effect of effective solar pathlength. A new parameterization is therefore proposed. It is emphasized that the atmospheric curvature effect on radiative transfer is a localized problem with height dependence. A method corresponding to the local effective pathlength factor is proposed. This rigorous scheme enables variations in both the pathlength and the gaseous amount along a solar direct beam to be accurately evaluated in the radiative transfer process. The results of the rigorous scheme can be used as the benchmark to the proposed parameterizations for the effective pathlength factor. It is found that the new parameterization proposed in this note has better results in flux and heating rates when compared to other parameterizations. 1. Theoretical background Radiative transfer in climate models is treated as a one-dimensional process accounting only for the vertical direction. However, within the one-dimensional limit, some realistic features of the atmosphere can still be addressed. In this note, we will discuss the effects of atmospheric curvature and refraction and their impact on the radiation calculation. If the atmosphere is planeparallel (PP) without spherical curvature, the solar beam pathlength in the atmosphere is H/ 0, where H is the height of the atmosphere and 0 cos 0, 0 is the solar zenith angle. Because of the spherical curvature of the atmosphere, when 0 is very large the solar beam pathlength could be significantly shorter than that of the PP case. Figure 1a shows the actual solar beam pathlength for the atmosphere with spherical curvature. For solar zenith angle 0, if there is no curvature, the solar beam pathlength from the top of the atmosphere (TOA) to the surface is l, but because of the atmospheric curvature the effective pathlength is l . When the solar zenith is small, l and l are almost the same, but for a large solar zenith angle, the difference between l and l could be very large, with l approaching infinity as 0 nears 90°. Since the problem is related to the curvature of the earth and the atmosphere, the effective pathlength should depend on the radius of the earth and considered thickness of the atmosphere. By defining 1/ e l /H, the following equation can be derived (Robinson 1966),
Effect of Fingolimod on the Frequency of Regulatory T Cells in Patients with Relapsing-Remitting Multiple Sclerosis

Multiple sclerosis (MS) likely results from an imbalance between pathogenic and regulatory T cells which lead to multifocal demyelination and axonal loss. Fingolimod, an immunomodulator, inhibits egress of lymphocytes from lymph nodes and their recirculation, approved for the treatment of MS. In this study, we investigated whether fingolimod affects immune imbalance occurred in MS patients through regulatory T (T reg) cells. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood obtained from 20 relapsing-remitting MS (RRMS) patients and 10 healthy subjects using Ficoll density centrifugation. Then, RRMS patients were treated by fingolimod (0.5 mg/day). After one month, blood samples were obtained from patients and PBMCs were isolated. The frequency of T reg cells in PBMCs from healthy subjects and MS patients before and after fingolimod therapy was measured by flow cytometry. Our data showed that the percentage of circulating T reg cells was significantly higher in MS patients than healthy subjects. Fingolimod had a potent effect on increasing T reg cell number in MS patients which this effect declined with age. These findings suggest that fingolimod can be considered as an effective therapeutic approach for modulating abnormal immune responses and restoring a balance between pathogenic and regulatory T cells in patients with MS.
Speaker dependent Malay phone recognition using continuous density hidden markov model

International Conference on Robotics, Vision, Signal Processing & Power Applications (ROVISP) is organized by School of Electrical and Electronic Engineering, Universiti Sains Malaysia. ROVISP 2016 welcomes researchers, scientists, engineers, academicians as well as industrial professionals from all around the globe to present their research results and development activities for oral or poster presentations.
Heart failure as a cause of hospitalization in IPF: comparison of the HFpEF, HFrEF, and PH

Background Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and fibrosing lung disease. Some IPF patients require hospitalization because of heart failure (HF), including HF with preserved ejection fraction (HFpEF), HF with reduced ejection fraction (HFrEF), and HF due to pulmonary hypertension (HFPH). However, the association between IPF and HF has not been clarified. We retrospectively investigated the clinical features and outcomes of patients with IPF and HF. Methods We examined the data of patients with IPF who were admitted for HF to the Kindai University Hospital from January 2008 to December 2018. Results During the study period, 37 patients with IPF were hospitalized because of HF. Among the 34 patients finally included in the study, 17 (50.0%) were diagnosed with HFpEF, 6 (17.6%) with HFrEF, and 11 (32.3%) with HFPH. Patients with HFrEF had significantly higher values for B-type natriuretic peptide (BNP), and left ventricular (LV) end-systolic and end-diastolic diameters than patients with HFpEF and HFPH (BNP: P = 0.01 and P = 0.0004, LV end-systolic diameter: P < 0.0001 and P < 0.0001, and LV end-diastolic diameter: P = 0.01 and P = 0.0004, respectively). Notably, the difference between the LVEFs of the patients with HFpEF or HFPH was not significant. The patients with HFpEF had the best 30-day mortality rate (0%, P = 0.02). Conclusions In patients with IPF, HFpEF is the most common type of HF that requires nonelective hospitalization. Patients with HFpEF survived longer than patients with the 2 other types of HF.
Impact of Over-Erupted Maxillary Third Molar on Attrition of Incisors: A Report of Two Cases

Cases of interocclusal space loss without an obvious reduction in the vertical dimension are difficult to manage. Herein, we present two cases of severe anterior tooth wear without an obvious reduction in the vertical dimension and with over-erupted left maxillary third molar(s). Occlusal contact features were analysed and chewing patterns were assessed using a series of electronic instruments. The over-erupted maxillary third molar caused a rotational chewing movement to the opposite side of the over-eruption, leading to abnormal loading on the anterior teeth, causing their heavy wear. Limitation of such a compensatory movement was observed in Case #2, with orofacial pain, but not in Case #1, without orofacial pain. The study proposes the role of posterior occlusion in severe anterior tooth wear and offers new insights into the role of modulating posterior occlusion for the prevention and functional treatment of anterior tooth wear.
Physical Stress and Determinants of Shooting Performance Among Norwegian Special Forces Operators

However, there is a lack of conceptual understanding of the factors influencing performance decrements in prone shooting. The present study examines how one can simulate a combat scenario by inducing acute physical stress, ultimately impacting one’s shooting performance (SP). The relationship between participants’ physical level and SP was measured in several ways. The SP of members of the Norwegian Navy Special Operations Forces (SOF) (N = 30) was measured before and directly after acute exercise-induced stress caused by a 200-m uphill run (90% HRmax). Under acute physical stress, participants took less time to fire five rounds (total 15.5 ± 10.9 s faster), and the probability of hitting the target was unaffected (92%). In terms of more sensitive measures, score was significantly reduced and shot-group dispersion increased (64 ± 90 cm2, p < 0.01, d = 0.72), mainly due to increased vertical dispersion (2.5 ± 4.6 cm, p < 0.01, d = 0.53). Age, trait somatic anxiety and the Big Five Inventory item “openness” explained 45.2% of the variance in shooting score in the pre-physical stress condition. In the post-physical stress condition, pre-test shooting score, the number of months deployed, and shooting time predicted 32.9% of the variance in shooting score. The change in SP (pre—post) showed the concentration disruption scale was the best predictor of the reduction in shot score (20.1%). From a practical point of view, maintaining the probability of hitting the target with reduced shooting time post-physical stress could be viewed as superior performance for SOF.
Differentiation of Protein Sequence Comparison Based on Biological and Theoretical Classification of Amino Acids in Six Groups

— The paper tries to differentiate results of protein sequence comparisons based on classification of amino acids in six groups, which are obtained either biologically or theoretically. In fact, biological classification of such groups of amino acids is known from their side chain properties. But recently same type of classification of amino acids in six groups with different constituent members has been obtained from similarity properties of amino acids, where these properties are determined theoretically from fuzzy representations of the amino acids. Natural query is to see whether there is any similarity or dissimilarity of the results of comparisons of same protein sequences based on the above different groups of classification. The paper proves that the results of comparisons are almost uniform in both the cases. Moreover in the later case the results are finer than those of the former one.
GWAS Identifies LINC01184/SLC12A2 as a Risk Locus for Skin and Soft Tissue Infections

Figure 1. Manhattan plot of results for the meta-analysis. Axes display the log10 transformed P-value by chromosomal position. The blue line indicates genome-wide‒suggestive associations (P < 1e-6), and the red line indicates genome-wide‒significant associations (P< 5e-8). Genome-wide‒significant loci ( 500 kb of lead variant) are highlighted in green. The image in the top right corner shows the quantile‒quantile plot. Axes display the observed (y-axis) and expected (x-axis) log10 transformed P-value. The black dots represent the observed P-values, whereas the red line represents the expected P-values under the null distribution. Genomic inflation factor (l) 1⁄4 1.01. TO THE EDITOR Microbial invasion of the skin and underlying soft tissues, known as skin and soft tissue infections (SSTIs), contribute to a considerable burden of disease worldwide (Kaye et al., 2019; Lozano et al., 2012). Knowledge about host factors contributing to SSTI risk is important to prevent the SSTIs. The genetics of SSTI susceptibility remain largely unknown, and the only previously published genome-wide study on SSTIs is a small family-based linkage study that did not identify significant linkage to any genes for erysipelas or cellulitis susceptibility (Hannula-Jouppi et al., 2013). A range of cardiometabolic risk factors has been associated with SSTIs (Butler-Laporte et al., 2020; Kaye et al., 2019; Winter-Jensen et al., 2020). Few studies have used genetic variants as instrumental variables (Mendelian randomization [MR]) to assess causality, which may reduce bias owing to reverse causation and confounding (Davies et al., 2018). Increasing body mass index has been found to increase the risk of SSTIs in such a framework (Butler-Laporte et al., 2020; Winter-Jensen et al., 2020), but other cardiometabolic risk factors have, to our knowledge, not been explored. The aims of this study were to conduct a GWAS on susceptibility to SSTIs, explore possible biological pathways through transcriptome-wide association analyses, and perform MR analyses to investigate the potential causal relationships of cardiometabolic risk factors with SSTIs. We used two independent cohorts: UK Biobank and Trøndelag Health Study (HUNT), where the UK Biobank
Posttranslational Regulation of a Leishmania HE XX H Metalloprotease (gp63) THE EFFECTS OF SITE-SPECIFIC MUTAGENESIS OF CATALYTIC, ZINC BINDING, N -GLYCOSYLATION, AND GLYCOSYL PHOSPHATIDYLINOSITOL ADDITION SITES ON N-TERMINAL END CLEAVAGE, INTRACELLULAR STABILITY, AND EXTRACELLULAR EXIT*

Leishmanolysin (EC 3.4.24.36) (gp63) is a HE XX H met- alloprotease, encoded by multicopied genes in Leishmania and implicated in the infectivity of these parasitic protozoa. We examined posttranslational regulation of gp63 expression by site-specific mutagenesis of the pre- dicted catalytic/zinc-binding sites in the H 264 E XX H motif, the potential sites of N -glycosylation and glycosyl phosphatidylinositol addition. Mutant and wild-type genes were cloned into a Leishmania -specific vector for transfecting a deficient variant, which produced gp63 (cid:59) 20-fold less than wild-type cells. The selective conditions chosen fully restored this deficiency in transfec- tants with the wild-type gene. Under these conditions, all transfectants were found comparable in both the plasmid copy number per cell and elevation of gp63 transcripts. Mutant and wild-type products in the trans- fectants were then compared quantitatively and qualitatively by specific immunologic and protease assays. The results indicate the following.
A potent PPARa agonist stimulates mitochondrial fatty acid b-oxidation in liver and skeletal muscle

Minnich, Anne, Nian Tian, Lisa Byan, and Glenda Bilder. A potent PPARa agonist stimulates mitochondrial fatty acid b-oxidation in liver and skeletal muscle. Am J Physiol Endocrinol Metab 280: E270–E279, 2001. —The proposed mechanism for the triglyceride (TG) lowering by fibrate drugs is via activation of the peroxisome proliferator-activated receptor-a (PPARa). Here we show that a PPARa agonist, ureido-fibrate-5 (UF-5), ;200-fold more potent than fenofibric acid, exerts TG-lowering effects (37%) in fat-fed hamsters after 3 days at 30 mg/kg. In addition to lowering hepatic apolipoprotein C-III (apoC-III) gene expression by ;60%, UF-5 induces hepatic mitochondrial carnitine palmitoyltransferase I (CPT I) expression. A 3-wk rising-dose treatment results in a greater TG-lowering effect (70%) at 15 mg/kg and a 2.3-fold elevation of muscle CPT I mRNA levels, as well as effects on hepatic gene expression. UF-5 also stimulated mitochondrial [H]palmitate b-oxidation in vitro in human hepatic and skeletal muscle cells 2.7and 1.6-fold, respectively, in a dose-related manner. These results suggest that, in addition to previously described effects of fibrates on apoC-III expression and on peroxisomal fatty acid (FA) b-oxidation, PPARa agonists stimulate mitochondrial FA b-oxidation in vivo in both liver and muscle. These observations suggest an important mechanism for the biological effects of PPARa agonists.
Application of Experimental Measurements in a Wind Tunnel to the Development of a Model for Aerodynamic Drag on Elite Slalom and Giant Slalom Alpine Skiers

Aerodynamic drag is a major cause of energy losses during alpine ski racing. Here we developed two models for monitoring the aerodynamic drag on elite alpine skiers in the technical disciplines. While 10 skiers assumed standard positions (high, middle, tuck) with exposure to different wind speeds (40, 60, and 80 km/h) in a wind tunnel, aerodynamic drag was assessed with a force plate, shoulder height with video-based kinematics, and cross-sectional area with interactive image segmentation. The two regression models developed had 3.9–7.7% coefficients of variation and 4.5–16.5% relative limits of agreement. The first was based on the product of the coefficient of aerodynamic drag and cross-sectional area (Cd·S) and the second on the coefficient of aerodynamic drag Cd and normalized cross-sectional area of the skier Sn, both expressed as a function of normalized shoulder height (hn). In addition, normative values for Cd (0.75 ± 0.09–1.17 ± 0.09), Sn (0.51 ± 0.03–0.99 ± 0.05), hn (0.48 ± 0.03–0.79 ± 0.02), and Cd·S (0.23 ± 0.03–0.66 ± 0.09 m2) were determined for the three different positions and wind speeds. Since the uncertainty in the determination of energy losses due to aerodynamic drag relative to total energy loss with these models is expected to be <2.5%, they provide a valuable tool for analysis of skiing performance.
Metasearch: Rank vs. Score Based Rank List Fusion Methods (without Training Data)

Given a set of rankings (a ranking is a linear ordering of a set of items), the task of ranking fusion is the problem of combining these lists in such a way to optimize the performance of the combination. The ranking fusion problem is encountered in many situations, one prominent of which is metasearch. It deals with the problem of combining the result lists returned by multiple search engines in response to a given query, where each item in a result list is ordered with respect to a search engine and query dependent relevance score. Several ranking fusion methods have been proposed in the literature. They can be classiﬁed based on whether: (i) they rely on the rank; (ii) they rely on the score; and (iii) they require training data or not. Preliminary experimental results seem to indicate that score based methods outperform rank based methods, while methods based on training data perform better than those without training data. In this paper we will compare rank and score based methods, without training data, in the context of metasearch. Our paper will make the following contributions: (i) we will report experimental results for the Markov chain rank based methods, for which no large experimental tests have yet been made; (ii) while it is believed that the rank based method, named Borda Count, is competing with score based methods, we will show that this is not true for metasearch; and (iii) we will show that Markov chain based methods compete with score based methods. This is especially important in the context of metasearch as scores are usually not available from the search engines.
CMAR_A_258182 10975..10984

1Department of Comprehensive Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China; 2Department of Day Care, Beijing Cancer Hospital, Beijing, People’s Republic of China; 3Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Objective: To investigate the effects of miR-490-3p on the proliferation, migration, invasion and apoptosis of lung adenocarcinoma (LUAD) cells through the Wnt/β-catenin signaling pathway. Methods: Differentially expressed miRNAs in LUAD tissues were analyzed by bioinformatics and the target miRNA went through GSEA enrichment analysis. qRT-PCR was used to detect the expression of miR-490-3p in human LUAD cells and normal bronchial cells. The constructed vectors were transfected into the LUAD cell lines using Lipofectamine 2000. Cell viability was detected by MTT, cell migration and invasion were detected by transwell assay, and cell apoptosis was detected by flow cytometry. Western blot was performed to detect the expression levels of the proteins related to the Wnt/β-catenin pathway and cell apoptosis. Xenograft tumor mouse models were used for in vivo validation. Results: The results of qRT-PCR showed that miR-490-3p was relatively lowly expressed in LUAD cells, and the expression level was different in different LUAD cell lines. The results of MTT, transwell and flow cytometry exhibited that miR-490-3p could significantly inhibit the proliferation, migration, invasion and increase cell apoptosis rate of LUAD cells. Western blot results showed that miR-490-3p promoted the expression of Bax, Caspase-3 and E-cadherin as well as the phosphorylation of GSK-3β and inhibited the expression of Bcl2, β-catenin and C-myc. Additionally, animal experiments were performed to prove that miR-490-3p suppressed LUAD malignant progression in vivo. Conclusion: MiR-490-3p inhibited the proliferation, migration, invasion and promoted the apoptosis of LUAD cells by down-regulating the Wnt/β-catenin signaling pathway, suggesting that miR-490-3p may be an indicator for early diagnosis and prognosis of LUAD.