Miracle-dz/UniFAR-FTUs huggingface dataset:
In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment

Introduction Colorectal cancer (CRC) is a type of cancer in humans that leads to high mortality and morbidity. CD166 and CD326 are immunoglobulins that are associated with cell migration. These molecules are included in tumorigenesis of CRC and serve a great marker of CRC stem cells. In the present study, we devised a novel chimeric protein including the V1-domain of the CD166 and two epitopes of CD326 to use in diagnostic or therapeutic applications. Methods In silico techniques were launched to characterize the properties and structure of the protein. We have predicted physicochemical properties, structures, stability, MHC class I binding properties and ligand-receptor interaction of this chimeric protein by means of computational bioinformatics tools and servers. The sequence of chimeric gene was optimized for expression in prokaryotic host using online tools and cloned into pET-28a plasmid. The recombinant pET28a was transformed into the E. coli BL21DE3. Expression of recombinant protein was examined by SDS-PAGE and Western blotting. Results The designed chimeric protein retained high stability and the same immunogenicity as of the original proteins. Bioinformatics data indicated that the epitopes of the synthetic chimeric protein might induce B-cell- and T-cell-mediated immune responses. Furthermore, a gene was synthesized using the codon bias of a prokaryotic expression system. This synthetic gene expressed a bacterial expression system. The recombinant protein with molecular weights of 27kDa was expressed and confirmed by anti-his Western blot analysis. Conclusion The designed recombinant protein may be useful as a CRC diagnostic tool and for developing a protective vaccine against CRC.
Human Resource Management Practices and Employees’ Engagement in Small and Medium Enterprises in Tanzania: The Mediating Effect of Organisation View

The SMEs sector is one of the important sectors in the economy characterized by high failure rate, weak ability to compete in the market and high labour turnover. SMEs need to engage their employees in order to address the mentioned challenges. With this fact, this article examined the mediation effect of organization view on the influence of HRM practices on employees’ engagement. The article involved 483 respondents and data were collected through questionnaire. Structural Equation Modeling (SEM) was used as data analysis technique. The results revealed that organization view partially mediated the influence of organisation support and employees’ communication on job engagement. Furthermore, the article revealed that organisation view mediated the influence of employees’ communication on organization engagement. Hence, among others, the article recommends that SMEs owners/operators should adopt “soft HRM” to determine the influence of organization support and employees’ communication on job engagement. They should also apply “soft HRM” to determine the influence of employees’ communication on organisation engagement.
Salvianolic Acid D Alleviates Cerebral Ischemia-Reperfusion Injury by Suppressing the Cytoplasmic Translocation and Release of HMGB1-Triggered NF-κB Activation to Inhibit Inflammatory Response

Inflammatory response participates in the overall pathophysiological process of stroke. It is a promising strategy to develop antistroke drugs targeting inflammation. This study is aimed at investigating the therapeutic effect and anti-inflammatory mechanism of salvianolic acid D (SalD) against cerebral ischemia/reperfusion (I/R) injury. A rat middle cerebral artery occlusion/reperfusion (MCAO/R) injury model was established, and an oxygen-glucose deprivation/reoxygenation (OGD/R) injury model was established in PC12 cells. Neurological deficit score, cerebral infarction, and edema were studied in vivo. Cell viability was achieved using the MTT method in vitro. The Bax, Bcl-2, cytochrome c, HMGB1, TLR4, TRAF6, NF-κB p65, p-NF-κB p65, and cleaved caspase-3 and -9 were tested via the Western blot method. Cytokines and cytokine mRNA, including TNF-α, IL-1β, and IL-6, were studied via ELISA and PCR methods. The translocation of HMGB1 and NF-κB were studied by immunofluorescence assay. The HMGB1/NeuN, HMGB1/GFAP, and HMGB1/Iba1 double staining was carried out to observe the localization of HMGB1 in different cells. Results showed that SalD alleviated neurological impairment, decreased cerebral infarction, and reduced edema in I/R rats. SalD improved OGD/R-downregulated PC12 cell viability. SalD also promoted Bcl-2 expression and suppressed Bax, cytochrome c, and cleaved caspase-3 and -9 expression. SalD decreased the intensity of TLR4, MyD88, and TRAF6 proteins both in vivo and in vitro, and significantly inhibited the NF-κB nuclear translocation induced by I/R and OGD/R. What's more, SalD inhibited HMGB1 cytoplasmic translocation in neurons, astrocytes, and microglia in both the cortex and hippocampus regions of I/R rats. In conclusion, SalD can alleviate I/R-induced cerebral injury in rats and increase the PC12 cell viability affected by OGD/R. The anti-inflammatory mechanism of SalD might result from the decreased nuclear-to-cytoplasmic translocation of HMGB1 and the inhibition on its downstream TLR4/MyD88/NF-κB signaling.
ASSESSMENT OF GENETIC VARIATION OF TILIA TOMENTOSA BY RAPD MARKERS

Tilia tomentosa varieties were analyzed using Random Amplified Polymorphic DNA (RAPD) primers to determine genetic diversity. In this study, six samples provided by the Botanical Garden “Anastasie Fătu” of Iasi, Romania were compared to a variety collected from an ecosystem in Giessen, Germany. A total of 91 bands were obtained from nine RAPD primers, 79 (86.8%) of which were polymorphic. Marker data was used for a principal component analysis that showed clear differentiation among Romanian and German samples. Furthermore, Romanian genotypes were clustered together in the principal component analysis. PCA analysis was followed by an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis which revealed two major groups. Samples from the Romanian ecosystem showed high genetic similarity. Generally, samples did not separate from each other in the UPGMA analysis, excepting the German sample.
Maraapunisaurus fragillimus, N.G. (formerly Amphicoelias fragillimus), a basal Rebbachisaurid from the Morrison Formation (Upper Jurassic) of Colorado

In 1878, Oramel Lucas shipped to E.D. Cope of the Academy of Natural Sciences of Philadelphia, a huge 1.5-m-tall neural spine from the dorsal vertebra of a sauropod (from the Upper Jurassic Morrison Formation) that Cope named and illustrated as Amphicoelis fragillimus.The holotype was lost and all that is known of the specimen is from Cope's original publication. Reanalysis of Cope's publication in light of other sauropods discovered since 1878 indicates that Amphicoelias fragillimus is a basal rebbachisaurid characterized by pneumatic neural spine and arch, and the unambiguous rebbachisaurid character of a festooned spinodiapophyseallamina. Because the specimen can no longer be referred to the basal diplodo­ coid Amphicoelias, the genus name is replaced with Maraapunisaurus n.g. As a rebbachisaurid, revised dimensions indicate a dorsal vertebra 2.4 m tall and a head-to-tail length for the animal of 30.3 to 32m, significantly less than previous estimates.
16S rRNA Gene sequencing: A practical approach to confirming the identity of food borne bacteria

One of the main challenges for clinical practice and public health surveillance is rapid and accurate identification of food borne pathogens. In Nigeria, the use of sophisticated molecular tools for foodborne pathogens detection has not been extensively adopted. Bacterial strains isolated from food products sold in Lagos, Nigeria, were subjected to phenotypic techniques and 16S rRNA gene sequencing for specie level identification. Bacterial identification was performed using colonial morphology, Gram staining, conventional biochemical tests, and 16S rRNA gene sequencing. The pairwise sequence similarities between all the bacterial species with official names were analysed. Out of the 30 bacterial strains isolated and sequenced, 29 (96.7%) strains had nucleotide identity to known bacterial species in the GenBank. One (3.3%) sequence contained a large number of undetermined nucleotides and was not associated with specific strain identification. This technique was efficient showing 96.6% of the isolates being identified to the genus level and 93.1% being identified to the species level. The strains that were not identified due to low similarity levels were assigned phylogenetic positions, suggesting that they may belong to new taxa. This study demonstrates that 16S rDNA sequence analysis is more accurate and objective for identification of foodborne pathogens and also offers the possibility of rapidly recognizing yet undescribed taxa. Keywords : Foodborne pathogens; Microbial detection; Phylogenetic relationship; Phenotypic identification; 16S rRNA gene sequencing
A Framework for Finding Outspread News Pattern on Diverse Dataset using Time-Series

Expressing feeling or opinion is an inherent property of the individual and Now a day’s social media becomes an integral part of everyone’s life. It is a great medium to analyze the feeling of mass, but sometimes it flows the false feeling in the form of fake news or contents posted on social media. These fake content affects the people in the form of sentiments or companies in the business loss/profit, because most of the people make opinions based on what they read on social media. In fact, fake news or false information can create the damage among the individual, so it should be identified as early as possible. The interest in finding the pattern of fake news has been growing very rapidly in the last few years. In this article we proposed a comprehensive pattern analysis of viral contents, real or fake news on twitter using time series analysis. The proposed technique is simple but effective for detecting and analysis fake contents on the social networks. Experiments results shows that our proposed technique outperformed for differentiating real vs fake news on twitter. Finally, we identify and discuss future direction.
HETRP: High Energy Efficient Trustable Routing Protocol for Wireless Sensor Network

Wireless sensor network environment based on limited resources technology. Energy is one of the most significant resources in such systems, so ideal utilization of energy is essential. A high energy efficient with trustable routingprotocol for Wireless_Sensor_Networks covered under this_paper. The protocol is trustworthy as far as data conveyance at the Base_Station. We assumed about portability in sensor nodes and in the base station. The proposed protocol depends on the cluster and hierarchical routing protocols. All clusters comprises of unique cluster-head-node and two-deputy-clusterhead-nodes, and several normal sensor-nodes. The cluster-head panel model introduced to optimize the re-clustering time and energy prerequisites. As consider the protocol trustworthiness, it lays finest exertion to guarantee a predetermined level of performance at the base-station. Contingent upon the network topology, transmit data from cluster head node to base station that done either by direct or indirect i.e. multi-hop way. Also, substitute ways are utilized for data transmission between a cluster head node and the base station. Thorough NS2 simulation-results delineate energy-efficiency, throughput, and delayed-lifetime of sensor-nodes affected by the proposedprotocol.
Modelling Simple and Efficient Data Transformation Scheme for Improving Natural Language Processing

The importance of natural language processing cannot be sided in the current era of communication and analytics where data is exponentially growing. Although, there has been various versions and schemes that has evolved in the past decade towards improving the performance of natural language processing, but still the problem towards precisely extracting the actual context is an open ended. Review of existing studies show a large scope of new work towards improving it. Therefore, this manuscript presents a unique simplified approach where natural language processing is carried out with a combined effort of syntactical and semantic based transformation scheme. The study is implemented using analytical methodology while the secondary motive of the work is also to balance the mining performance as well as optimizing storage performance too. The study outcome shows proposed scheme to excel better performance with respect to time duration in all the internal processes being involved for data transformation.
Design, Modelling and Control of Novel Series-Elastic Actuators for Industrial Robots

This paper describes data-driven modelling methods and their use for the control of a novel set of series-elastic actuators (SEAs). A set of elastic actuators was developed in order to fulfill the end-user needs for tailored industrial collaborative robot manipulators of different morphologies and payloads. Three different types of elastic actuation were investigated, namely, disc springs, coil springs and torsion bars. The developed algorithms were validated both on single actuators and on a 6-DOF robotic arm composed of such actuators.
A Comparative Analysis of Clinical Screening Test and Language Specific Test in Language Delay Children

The aim of this study was to investigate the usefulness of a clinical screening test [the Korean Infant and Child Developmental Test (KICDT)] compared to language specific tests: the sequenced language scale for infant (SELSI) and the Preschool Receptive-Expressive language Scale (PRES) in children with delayed language development. A retrospective chart review was conducted on 615 children who visited the Department of Pediatrics at Chonbuk National University Hospital from January 2013 to December 2016. All patients were evaluated with KICDT as a clinical screening test and SELSI or PRES as a language specific test. Language Developmental Quotients (LDQs) from the KICDT were compared with the Receptive Language Quotient (RLQ) and expressive language quotient (ELQ) from the SELSI or PRES. The sensitivity, specificity and predictive values of LDQ of KICDT were calculated by comparing with SELSI/PRES. Language DQs from the KICDT were significantly correlated with the RLQ (r=0.706), ELQ (r=0.768), and total language quotient (TLQ) (r=0.766) from the SELSI/PRES (p<0.05). In cross tabulation, the patients belonging to the retardation groups in both KICDT and SELSI/PRES were 417 (67.8%). Otherwise, patients belonging to the normal group in KICDT but not in SELSI/PRES were 151 (24.6%). Sensitivity and specificity of LDQ of KICDT relative to SELSI/PRES were 72.3% and 92.2% respectively (p<0.05). Our data suggests that clinical screening tests alone, not cumbersome language specific tests, can determine language developmental delays in children.
The Effect of Consumer Value on Attitude Toward Green Product and Green Consumer Behavior in Organic Food

At the moment the world market continues to change, sustainability pillars such as environment, economy and social justice have become part of marketing decisionmaking. Developing a marketing strategy in the midst of current environmental and economic issues has led to a focus on the importance of green marketing. Using the theory of consumption values and value-attitude-behavior model, this research proposes to explore the green consumer behavior for green products in Indonesia. Attitude toward green product and consumer values such as functional value (price and quality), social value, conditional value, epistemic value, emotional value, and environmental value were used to study the green consumer behavior for green products. This study developed a research model and empirically tested by collecting data from questionnaires that were distributed in Indonesia. Based on a sample of 200 respondents, the results indicate that only social value has a positive impact on green consumer behavior, functional value (price), functional value (quality), environmental value, conditional value, epistemic value, and emotional value do not influence green consumer behavior. As a mediator, attitude toward green product has a significant effect on epistemic value, environmental value, and emotional value. This confirms and significantly adds to the literature of green consumer behavior in a developing market. Keywords―Green Products, Green Consumer Behavior, Value-Attitude-Behavior Model, Theory of Consumption
Effects of food sources on the fecundity and gene expression of vitellogenin and its receptor from Amblyseius eharai (Acari: Phytoseiidae)

Abstract Vitellogenin (Vg) and Vitellogenin receptor (VgR) play key roles in the reproductive process and development of many arthropod species, including Amblyseius eharai, a dominant natural enemy of spider mites and other small pests. In this study, impacts of different food on A. eharai fecundity were investigated. Two Vg genes (AeVg1 and AeVg2) and AeVgR genes were cloned, with their expression pattern analyzed. When fed with different foods including Tetranychus cinnabarinus, Aleuroglyphus ovatus and Camellia oleifera pollen, the number of eggs for each female of A. eharai was influenced significantly (P<0.05), while the hatching rate of eggs not significantly (P>0.05). The open reading frames of AeVg1, AeVg2 and AeVgR were 5673, 5634 and 5597 bp, encoding 1857, 1851 and 1830 amino acids, respectively. Phylogenetic analysis of the amino acid sequences between the AeVgs and AeVgR and 35 other Vg and VgRs from other arthropod species showed that A. eharai AeVg1 was related most closely to Neoseiulus barkeri Vg1, AeVg2 was related most closely to Neoseiulus cucumeris Vg2, AeVgR was most similar to Varroa jacobsori VgR. AeVgs and AeVgR had similar expression patterns: the highest expression was observed in the oviposition female stage, and the highest expression was observed in A. eharai fed with C. oleifera pollen. Positive correlations between expression of AeVgs and AeVgR and fecundity were observed. These results will help us understand the molecular mechanism of the reproductive capacity in A. eharai.
Rickettsial survey and ticks infesting small mammals from the Amazon forest in midwestern Brazil

Abstract In the present study, samples of tick, tissue and blood from free-living wild small mammals (rodents and marsupials) captured in two municipalities from the Amazon biome of the Mato Grosso state, were molecularly tested for rickettsial infection. A total of 148 mammal specimens (46 rodents and 102 marsupials) were trapped, and 2,700 Amblyomma ticks (2,000 larvae and 700 nymphs) were collected. Ticks were recognized into four Amblyomma species: Amblyomma cajennense sensu stricto, Amblyomma coelebs, Amblyomma humerale, and Amblyomma scalpturatum, with new data for tick-host associations from small mammals in the Amazon biome. All tissue and blood samples of small mammals tested by PCR for rickettsial detection were negative. However, concerning tick-Rickettsia associations, Rickettsia amblyommatis was detected in all four tick species identified in the present study, being the first record for A. scalpturatum ticks. Furthermore, an uncharacterized species of Rickettsia belonging to the spotted fever group was detected in A. humerale as well.
Characterization of the complete mitochondrial genome sequences of three Merulinidae corals and novel insights into the phylogenetics

Over the past few decades, modern coral taxonomy, combining morphology and molecular sequence data, has resolved many long-standing questions about scleractinian corals. In this study, we sequenced the complete mitochondrial genomes of three Merulinidae corals (Dipsastraea rotumana, Favites pentagona, and Hydnophora exesa) for the first time using next-generation sequencing. The obtained mitogenome sequences ranged from 16,466 bp (D. rotumana) to 18,006 bp (F. pentagona) in length, and included 13 unique protein-coding genes (PCGs), two transfer RNA genes, and two ribosomal RNA genes . Gene arrangement, nucleotide composition, and nucleotide bias of the three Merulinidae corals were canonically identical to each other and consistent with other scleractinian corals. We performed a Bayesian phylogenetic reconstruction based on 13 protein-coding sequences of 86 Scleractinia species. The results showed that the family Merulinidae was conventionally nested within the robust branch, with H. exesa clustered closely with F. pentagona and D. rotumana clustered closely with Favites abdita. This study provides novel insight into the phylogenetics of species within the family Merulinidae and the evolutionary relationships among different Scleractinia genera.
Evaluating the role of FAMIly history of cancer and diagnosis of multiple neoplasms in cancer patients receiving PD-1/PD-L1 checkpoint inhibitors: the multicenter FAMI-L1 study

ABSTRACT Background: We investigate the role of family history of cancer (FHC) and diagnosis of metachronous and/or synchronous multiple neoplasms (MN), during anti-PD-1/PD-L1 immunotherapy. Design: This was a multicenter retrospective study of advanced cancer patients treated with anti-PD-1/PD-L1 immunotherapy. FHC was collected in lineal and collateral lines, and patients were categorized as follows: FHC-high (in case of cancer diagnoses in both the lineal and collateral family lines), FHC-low (in case of cancer diagnoses in only one family line), and FHC-negative. Patients were also categorized according to the diagnosis of MN as follows: MN-high (>2 malignancies), MN-low (two malignancies), and MN-negative. Objective response rate (ORR), progression-free survival (PFS), overall survival (OS), and incidence of immune-related adverse events (irAEs) of any grade were evaluated. Results: 822 consecutive patients were evaluated. 458 patients (55.7%) were FHC-negative, 289 (35.2%) were FHC-low, and 75 (9.1%) FHC-high, respectively. 29 (3.5%) had a diagnosis of synchronous MN and 94 (11.4%) of metachronous MN. 108 (13.2%) and 15 (1.8%) patients were MN-low and MN-high, respectively. The median follow-up was 15.6 months. No significant differences were found regarding ORR among subgroups. FHC-high patients had a significantly longer PFS (hazard ratio [HR] = 0.69 [95% CI: 0.48–0.97], p = .0379) and OS (HR = 0.61 [95% CI: 0.39–0.93], p = .0210), when compared to FHC-negative patients. FHC-high was confirmed as an independent predictor for PFS and OS at multivariate analysis. No significant differences were found according to MN categories. FHC-high patients had a significantly higher incidence of irAEs of any grade, compared to FHC-negative patients (p = .0012). Conclusions: FHC-high patients seem to benefit more than FHC-negative patients from anti-PD-1/PD-L1 checkpoint inhibitors.
Inhibitory and preventive effects of Lactobacillus plantarum FB-T9 on dental caries in rats

ABSTRACT Streptococcus mutans is recognized as the main cause of dental caries, and the formation of a plaque biofilm is required for caries development. This study aimed to determine the inhibitory effect of Lactobacillus plantarum FB-T9 on S. mutans biofilm formation in vitro and on the prevention and treatment of dental caries in rats. During in vitro experiments, FB-T9 exhibited good bacteriostatic ability in a plate competition assay. This strain also significantly reduced the biomass and viability of S. mutans biofilms and induced structural damage during the early (6 h), middle (12 h) and late (24 h) stages of biofilm formation. In a 70-day in vivo experiment, FB-T9 significantly reduced the levels of S. mutans on the dental surfaces of rats by more than 2 orders of magnitude of the levels in the dental caries model group (p < 0.05). Moreover, FB-T9 significantly reduced the caries scores (modified Keyes scoring method) in both the prevention and treatment groups (p < 0.05) and had great colonization potential in the oral cavity. These results indicate the potential usefulness of L. plantarum FB-T9 as a probiotic for the prevention and treatment of caries.