Miracle-dz/UniFAR-FTUs huggingface dataset:
Rehydration of freeze substituted brain tissue for pre-embedding immunoelectron microscopy

Electron microscopy (EM) volume reconstruction is a powerful tool for investigating the fundamental structure of brain circuits, but the full potential of this technique is limited by the difficulty of integrating molecular information. High quality ultrastructural preservation is necessary for EM reconstruction, and intact, highly contrasted cell membranes are essential for following small neuronal processes through serial sections. Unfortunately, the antibody labeling methods used to identify most endogenous molecules result in compromised morphology, especially of membranes. Cryofixation can produce superior morphological preservation and has the additional advantage of allowing indefinite storage of valuable samples. We have developed a method based on cryofixation that allows sensitive immunolabeling of endogenous molecules, preserves excellent ultrastructure, and is compatible with high-contrast staining for serial EM reconstruction.
Outsourcing: decision-making methods and criteria during design and engineering

Research to date for decision-making on outsourcing has almost exclusively focussed on strategic and operational levels. Consequently, we know relatively little about decision-making on outsourcing during design and engineering, i.e. the tactical level; that also means that the characteristics of design and engineering – incomplete and inaccurate information and progressive availability of data – are not incorporated in existing frameworks and methods at that level. To fill this gap, a first-of-its-kind framework, based on an extensive appraisal of the literature, is validated; to that purpose, a Delphi survey was undertaken gauging the opinions of two homogeneous groups of academic and industry experts. The analysis of the survey affirms the assumptions made about the role of the progressive availability of data during decision-making on outsourcing. The results from this study should not only inform further research into decision-making during design and engineering but potentially the framework might also have validity for purchasing at the tactical level and strategic decision-making on outsourcing.
25-5 Kwe Microturbine Design Aspects

In concert with the growing emphasis placed on distributed power generation there will be a need, in the first decade of the 21st century, for a compact thermal energy system capable of providing the total energy needs of individual homes. A natural gas fueled cogeneration microturbine with ultra low emissions will meet this need.The results of a study to assess the thermal and economic aspects of microturbines in the 25 to 5 Kwe range are presented. It was determined that with current state of art component peak performance levels, and metallic materials, thermal efficiency goals of 27 and 22.5% respectively at sea level standard day conditions are attainable. Higher overall thermal efficiencies of 60% are attainable with a microturbine combined with a fuel cell, and approaching 80% combined with exhaust fired boilers.Development of a low cost natural gas combustor injection method, combined with a low cost high effectiveness recuperator were considered mandatory for market acceptance.Copyright © 2000 by ASME
Steering vehicle control and road bank angle estimation: application for diagnosis of vehicle limits in bend

This paper deals with the diagnosis of the critical driving situations. This work is divided into three parts: the first one presents two steering controllers using the sliding mode and the switched H∞ controllers, the second one describes an unknown input sliding mode observer, while the last one presents an approach to the diagnosis of critical driving situations. All existence conditions are established using the Lyapunov approach. Simulations are conducted to highlight the efficiency of the proposed approach using the experimental data recorded by an instrumented Peugeot 307 laboratory car. The diagnosis of the critical driving situations is achieved by the speed extrapolation concept using simultaneously a non–linear model, a steering control and road bank observer. The speed extrapolation concept is used in order to evaluate situations under high dynamic loads on a bend, using stability of the sideslip motion.
One-Dimensional Disordered Bosonic Systems

Disorder is everywhere in nature and it has a fundamental impact on the behavior of many quantum systems. The presence of a small amount of disorder, in fact, can dramatically change the coherence and transport properties of a system. Despite the growing interest in this topic, a complete understanding of the issue is still missing. An open question, for example, is the description of the interplay of disorder and interactions, which has been predicted to give rise to exotic states of matter such as quantum glasses or many-body localization. In this review, we will present an overview of experimental observations with disordered quantum gases, focused on one-dimensional bosons, and we will connect them with theoretical predictions.
Recent Developments in the Field of Modified Patankar‐Runge‐Kutta‐methods

Modified Patankar‐Runge‐Kutta (MPRK) schemes are numerical one‐step methods for the solution of positive and conservative production‐destruction systems (PDS). They adapt explicit Runge‐Kutta schemes in a way to ensure positivity and conservation of the numerical approximation irrespective of the chosen time step size. Due to nonlinear relationships between the next and current iterate, the stability analysis for such schemes is lacking. In this work, we introduce a strategy to analyze the MPRK22(α)‐schemes in the case of positive and conservative PDS. Thereby, we point out that a usual stability analysis based on Dahlquist's equation is not possible in order to understand the properties of this class of schemes.
BUCKLING AND POST-BUCKLING OF ISOTROPIC AND COMPOSITE STIFFENED PANELS: A REVIEW ON OPTIMISATION (2000-2015)

Stiffened panels made out of isotropic or anisotropic materials are being extensively used as structural elements for aircraft, maritime, and other structures. In order to maintain stiffness and strength with light weight, new design techniques must be employed when utilising these materials. Their stability, ultimate strength and loading capacity are the key issues pertaining to these engineering structures which have attracted a number of investigators to undertake in- depth research, either in an academic or actual engineering context. This paper presents a review of the optimisation techniques applied to buckling and post-buckling of stiffened panels. Papers published in the period from 2000 to May 2015 have been taken into consideration. The topic is addressed by identifying the most significant objectives, targets and issues, as well as the optimisation formulations, optimisation algorithms and models available. Finally a critical discussion, giving some practical advice and pointing out and post-buckling of stiffened panels, is provided.
Research on the rapid design technology of automotive inspection structure based on MBD model

In view of the specificity and low efficiency of the design of automobile inspection fixture, a deformation design method of inspection fixture based on BP neural network algorithm is proposed. BP neural network algorithm is used to realize the learning and classification of case knowledge, and FCM (fuzzy c-means) algorithm and kernel principal component analysis are used to optimize the information source to improve the retrieval efficiency and accuracy. Based on the analysis of the existing fixture design case structure, the case structure is skeletonized to increase the applicability of the case structure. At the same time, the case frame structure is associated with the size chain, the priority deformation rule is proposed, and the relationship of each size chain is established to realize the mutual adjustment of each size chain. From the similarity of retrieval cases, the paper proposes the design scheme of inspection tools to improve the design efficiency. Finally, taking the front bumper model as the experimental object, the deformation rules are compared, and the priority deformation rule is more accurate than the ordinary basic rule. Compared with the manual design, the design efficiency of this method is improved by 55.71%, which proves the feasibility of this method.
ANALYSIS OF OIL FILM TEMPERATURE DISTRIBUTION OF THE HIGH SPEED SHAFT JOURNAL BEARINGS OF GEAR BOX APPLIED IN THE COAL MINE HOISTING MACHINE

The paper describes the analysis of oil film temperature distributions that are generated in the gear train journal bearings of high speed shaft. The oil film temperature distributions were obtained on the assumption of adiabatic, laminar oil film for the finite bearing length, parallel axis of journal and bush and radial load of bearings. Maximum oil film temperature was determined based on the obtained oil film temperature distributions. The calculation were carried-out on the condition of static equilibrium position of journal.Copyright © 2005 by ASME
Corporate Internal Management Information System

The system is researched and developed in combination with the current development of computer technology, to meet the demands of the medium and small-sized enterprises for informatization of internal management. The system is constructed by use of JavaBean technology for JSP dynamic webpage making. It is designed in B/S development model, and adopts Tomcat as JSP server, and MyEclipse as the development environment, while DB 8.2 is used for the backend database. The major part of the system is constructed in the structure of customer end—middle layer (module layer) ---data base. The system administrator can increase, delete, revise and query the information, while the common users cannot take information retrieval. It has been proved by practice that the system developed corresponds to the demands of the enterprises for internal management and has a great application prospect.
Lessons Learned by Rehabilitation Counselors and Physicians in Services to COVID-19 Long-Haulers: A Qualitative Study

Coronavirus disease (COVID-19) may cause long-lasting adverse consequences after acute recovery, including functional limitations and reduced work capacity. Individuals with long-lasting complications of COVID-19 are known as long-haulers. There is a knowledge gap on how COVID-19 complications affect return-to-work (RTW) efforts. We aimed to describe the challenges that long-haulers encounter when returning to work from the perspectives of rehabilitation professionals. In this study, four certified rehabilitation counselors and four rehabilitation physicians in U.S. outpatient rehabilitation centers participated in focus groups. Participants discussed challenges in working with long-haulers, including personal attributes, post–COVID-19 symptoms and complications, uncertain recovery and unpredictable outcomes, limited health care accessibility and support, and unsupportive work environments. Participants provide individualized services to meet long-haulers’ diverse needs as they do for all persons with disabilities, although they expressed uncertainty due to the variable disease course and risk of infection. Modifying workplace policies, especially gradual RTW, were frequently mentioned accommodations. The findings highlight the RTW issues of long-haulers. We provide recommendations on increasing awareness of the challenges and job accommodations of long-haulers.
A Dynamic and Customisable Layered Serious Game Design Framework for Improving the Physical and Mental Health of the Aged and the Infirm

This paper proposes a dynamic and customizable layered serious game design framework for improving the physical and mental health of the aged after presen ting the results obtained from a study with mainstream and alternative/complimentary health professionals concerning the usage of interactive games as a tool to improve bot h physical and mental well-being of the elderly. This study re ports on the commonality of design and health factors regarding the usability of video games for the aged to ensure the elderly benefit from traditional and alternative healthcare professionals' perspectives.
Development of an underwater glider equipped with an auxiliary propulsion module

A low power propeller based propulsion module has been developed to complement the buoyancy engine of a 200 m Slocum electric glider. This device is introduced to allow new behaviours such as horizontal flight and faster overall speeds to expand the existing operational envelope of underwater gliders. The design goal is to match typical horizontal glider speeds of 0.3 m/s while minimising the impact of the module on the performance of the unmodified glider. After careful selection of the propeller and motor candidates the stand-alone propulsion module has been tested in a small flume tank to verify the system's performance. Since the desired flight trajectory is restricted to the horizontal plane the validity of a previously published hydrodynamic model of the glider at zero angle of attack was verified by conducting drag measurements at various flow velocities at full scale in a larger flume tank. Self propulsion tests were also performed to establish the performance of the glider with the new propulsion module in a larger flume tank and in a towing tank. Open water tests were performed in a large test tank to show the stability of the platform for horizontal flight. The results from these tests show that the new propulsion module is capable of driving the vehicle horizontally while matching the performance of the conventional glider.
Application of Nonparametric Optimization Methods for Dyeing of Wool

3 Abstract: In this study, The preparation and production of multilamellar liposome from Soya lecithin with 75% phosphatidylcholine were carried out and the behavior of liposome in dye-bath at different temperature, time, Sodium Sulphate, pH and concentration (five factors) were considered and compared by two different optimization approaches, namely, parametric and nonparametric using Box-Behnken standard design matrix of Response Surface Methodology (RSM) in both cases to produce representative data. The comparative results show that when parametric model is not a good fit for data, nonparametric approach can be considered as one of the proper alternatives.
Complete transient simulation of flash EEPROM devices

It has been demonstrated that the Fowler-Nordheim tunneling, hot electron injection, and band-to-band tunneling current models incorporated into the 2D device simulator HFIELDS have allowed an accurate simulation and analysis of the flash EEPROM cell during the transient writing cycles. These represent all the important physical mechanisms which occur during the writing of flash EEPROM devices. The physical models have been verified by comparison with flash EEPROM devices fabricated using a 1.0 mu m technology. <<ETX>>
On the approximation of the optimum detector by the energy detector in detection of colored Gaussian signals in noise

We demonstrate that the optimum detector of colored Gaussian signals in white Gaussian noise can be approximated, without serious degradation in detection performance, by the so-called energy detector which is optimum only when the signal is also white. Contrary to intuition, such an approximation is possible when the signal which is colored dominates the noise which is white. The demonstration is made analytically by deriving the optimum detection statistic and also numerically by simulating the detection performance of both detectors. A comparison of data processing complexity of the two detectors is also given.
Carrier transport related analysis of high-power AlGaN/GaN HEMT structures

Shubnikov-de Haas (SdH) oscillation and Hall measurement results were compared with HEMT DC and RF characteristics for two different MOCVD grown AlGaN-GaN HEMT structures on semiinsulating 4H-SiC substrates. A HEMT with a 40-nm, highly doped AlGaN cap layer exhibited an electron mobility of 1500 cm/sup 2//V/s and a sheet concentration of 9/spl times/10/sup 12/ cm at 300 K (7900 cm/sup 2//V/s and 8/spl times/10/sup 12/ cm/sup -2/ at 80 K), but showed a high threshold voltage and high DC output conductance. A 27-nm AlGaN cap with a thinner, lightly doped donor layer yielded similar Hall values, but lower threshold voltage and output conductance and demonstrated a high CW power density of 6.9 W/mm at 10 GHz. The 2DEG of this improved structure had a sheet concentration of n/sub SdH/=7.8/spl times/10/sup 12/ cm/sup -2/ and a high quantum scattering lifetime of /spl tau//sub q/=1.5/spl times/10/sup -13/ s at 4.2 K compared to n/sub SdH/=8.24/spl times/10/sup 12/ cm/sup -2/ and /spl tau//sub q/=1.72/spl times/10/sup -13/ s for the thick AlGaN cap layer structure, Despite the excellent characteristics of the films, the SdH oscillations still indicate a slight parallel conduction and a weak localization of electrons. These results indicate that good channel quality and high sheet carrier density are not the only HEMT attributes required for good transistor performance.
3D Shape Reasoning for Identifying Anatomical Landmarks

Orthopaedic surgeries require identification of anatomical landmarks on skeletal tissue. Manual palpation methods may not be accurate, especially in resecting deceased tissue and positioning custom implants or mega endo-prostheses. This article describes a computer-aided method for automatic identification of anatomical landmarks on a 3D model reconstructed from CT images of the patient. This is based on computing curvature values, identifying different regions of the surface (generated from the segmented volume data), and classifying the regions as peaks, ridges, pits and ravines. The landmarks are identified by walking along all vertices, extracting gradients from curvature map, and checking against a set of rules. The approach has been successfully demonstrated on a pelvis model reconstructed from 443 slices. The locations of the landmarks are determined, useful for dimension measurements and pre-operative planning.
Rising from the Ashes of Electoral Defeat : Reversal of Vlaams Belang’s Electoral Fortune in the Light of Supply-Side Explanation

Within the last few years, radical right-wing parties gained relevance in some European countries, shifting the position of marginal political rebel to being a serious competitor of the mainstream parties� To explain the emergence and volatile fortune of this party family, we resort to supply-side explanation, and we analyse the case of the Flemish Vlaams Belang (VB) which suffered severe electoral defeat in 2014, to be reborn five years later� The analysis is based on the existing data concerning party platform, shift in electoral support, campaign spending, and the findings from individual in-depth interviews conducted with people from party headquarter — ABSTRAKT —
Optimization in Manual Aiming: Relating Inherent Variability and Target Size, and Its Influence on Tendency

Abstract For manual aiming, the optimized submovement model predicts a tendency toward target-center of primary movement endpoints (probabilistic strategy), while the minimization model predicts target undershooting (“play-it-safe” strategy). The spatial variability of primary movement endpoints directed toward a cross-hair (400–500 ms) (Session 1) were scaled by a multiplicative factor (x1 − 4) to form circular targets of different sizes (Session 2). In recognition of both models, it was predicted that the more that inherent variability exceeded the target size, the greater the tendency to shift from target-center aiming to target undershooting. The central tendency of primary movement endpoints was not influenced by the targets, while it neared target-center. These findings concur with a probabilistic strategy, although we speculate on factors that might otherwise foster a “play-it-safe” strategy.
High-level low power FPGA design methodology

High-level design for low power is difficult to accomplish especially for FPGA designs. Presents a design technique that uses pre-computed tables that characterize the RTL and Intellectual Property (IF) components to estimate power. Actual tables were computed and the low-power design technique demonstrated. The results show that a lower power design can be achieved given this design methodology.
Seismic fragility methodology for highway bridges using a component level approach

Bridge fragility curves, which express the probability of a bridge reaching a certain damage state for a given ground motion parameter, play an important role in the overall seismic risk assessment of a transportation network. Current analytical methodologies for generating bridge fragility curves do not adequately account for all major contributing bridge components. Studies have shown that for some bridge types, neglecting to account for all of these components can lead to a misrepresentation of the bridges' overall fragilities.
MicroRNA-573 inhibits cell proliferation, migration, and invasion and is downregulated by PICSAR in cutaneous squamous cell carcinoma

The incidence of cutaneous squamous cell carcinoma (cSCC) has been increasing in recent years. Meanwhile, microRNAs have been found to play vital roles in various cancers, including cSCC. This study aimed to investigate the expression of microRNA-573 (miR-573) in cSCC, its relationship with long non-coding RNA PICSAR and analyze its biological role. The relationship between PICSAR and miR-573 was confirmed by dual-luciferase reporter assay and Pearson’s correlation coefficient analysis. The levels of PICSAR and miR-573 were measured using quantitative Real-Time polymerase chain reaction. Cell Counting Kit-8 assay was used to evaluate the cSCC cell proliferation ability. The migration and invasion abilities of cSCC cells were evaluated by Transwell assay. PICSAR expression was increased and miR-573 was decreased in tumor tissues and cSCC cell lines. PICSAR and miR-573 can bind directly, and miR-573 expression was downregulated by PICSAR in cSCC. Overexpression of miR-573 significantly inhibited the proliferation, migration, and invasion abilities of A431 and SCC13 cells. In addition, miR-573 overexpression reversed the promotion effects of PICSAR overexpression on cSCC cell proliferation, migration, and invasion abilities. In conclusion, our findings indicated that miR-573 expression was decreased in tumor tissues and cSCC cells and was downregulated by PICSAR in cSCC. Additionally, miR-573 overexpression inhibited cSCC cell proliferation, migration and invasion, and reversed the promotion effects of PICSAR overexpression on cSCC cell biological functions. Thus, miR-573 might function as a tumor suppressor and might be involved in the regulatory effects of PICSAR on tumorigenesis in cSCC.
Hardware emulation of multi-level decision feedback equalization

The bit error rate (BER) performance of a multi-level decision feedback equalizer (MDFE) for a magnetic recording channel is evaluated. The unique equalization scheme designed for 2/3(1,7) RLL codes was emulated on the RAM-DFE IC. Measurements with a synchronous clock at 54 Msps demonstrated that raising the inner target levels improves the performance considerably. It was found that MDFE outperforms conventional DFE at the same symbol density of PW50=2.5 T and yields similar performance at the same user density of 1.78.