Miracle-dz/UniFAR-FTUs huggingface dataset:
The complete plastid genome of Lilium regale E.H.Wilson

Abstract Lilium regale E.H. Wilson is a native lily species in western Sichuan of China and an important resource for lily breeding. In this study, the plastid genome of L. regale was assembled de novo using the next-generation sequencing data. The plastid genome of L. regale was 152,998 bp in length, with a typical quadripartite circle structure consisting of a small single-copy region of 17,529 bp, a large single-copy region of 82,375 bp, and a pair of inverted repeats of 26,547 bp each. A total of 137 different genes were predicted, including 84 protein-coding genes, 38 transfer RNA genes, 8 ribosomal RNA genes, and 7 pseudogenes. The overall GC content of the plastid genome was 36.98%. Phylogenetic analysis revealed that L. regale is most closely related to Lilium leucanthum.
The published complete mitochondrial genome of the milk shark (Rhizoprionodon acutus) is a misidentified Pacific spadenose shark (Scoliodon macrorhynchos) (Chondrichthyes: Carcharhiniformes)

Abstract The recently published mitogenome of milk shark Rhizoprionodon acutus (MN602076/NC_046016) was fully resolved in an unexpected phylogenetic position in the original mitogenome announcement, which rendered the genus Scoliodon paraphyletic. Here, we show that this mitogenome is actually that of a misidentified Pacific spadenose shark (Scoliodon macrorhynchos). The error is documented to avoid the perpetuation of erroneous sequence information in the literature.
The complete mitochondrial genome sequence of Dodona eugenes (Lepidoptera: Riodinidae)

Abstract The complete mitochondrial genome (mitogenome) sequence of Dodona eugenes (Lepidoptera: Riodinidae) was determined and analyzed. The mitogenome is 15,680 bp in length with consisting of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes (rrnL and rrnS), and one AT-rich region. The gene content, orientation, and order are identical to that of the majority of other lepidopteran insects. The D. eugenes mitogenome includes a cox1 gene with an atypical CGA(R) start codon and three genes (cox1, nad5, and nad4) exhibiting incomplete stop codons. All tRNAs have a typical secondary cloverleaf structure, except for trnS1 which lacks the dihydrouridine (DHU) arm. The 825-bp long AT-rich region is the longest among sequenced riodinids, which range from 349 to 423 bp. The conclusion of phylogenetic analysis highly supported the monophyly of Riodinidae, which is standing as the sister of the family Lycaenidae.
Study on Potential of Meridian Acupoints of Traditional Chinese Medicine

In recent years, Traditional Chinese Medicine (TCM) has attracted more and more attention due to its good therapeutic effect, low cost, and convenience. This research is also a part of the goal of the modernization of TCM. Based on the meridian electric potential acquisition system independently developed by our project team, in this paper, we designed the human body's meridian electric potential acquisition scheme. We use principal component analysis (PCA) to prove that the meridional potential signal is derived from the ECG signal. Then, Inception ResNet V2 was used to classify acupoints and nonacupoints. Finally, the classification accuracy rate reached 86.59045265, and the F1 score = 0.72161642. This shows that acupoints and nonacupoints can be distinguished by their surface potential.
Identification of miR-145 as a regulator of the cardiomyocyte inflammatory response and oxidative stress under hyperglycemia

The current study aimed to explore the effects of microRNA (miR)-145 on the inflammatory response and oxidative stress (OS) in high glucose (HG)-induced cardiomyocytes, as well as the specific mechanism underlying this action. H9c2 cells were treated with 33 mmol/l glucose (HG group) or cotreated with 24.5 mmol/l mannitol and 5.5 mmol/l glucose (hypertonic group), and the expression levels of miR-145 and ADP ribosylation factor 6 (ARF6) were detected. The cells were transfected with pcDNA3.1-ARF6, miR-145 mimics or corresponding negative controls prior to the assessment of cell survival rate. Levels of lactate dehydrogenase (LDH), reactive oxygen species (ROS) and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and the levels of IL-6, TNF-α and monocyte chemoattractant protein-1 (MCP-1) were subsequently determined. The apoptotic rate of H9c2 cells was examined by flow cytometry. The interaction between miR-145-ARF6 was predicted and confirmed by luciferase reporter assays. In the HG group, miR-145 expression was significantly decreased and ARF6 expression significantly increased compared with controls. Furthermore, the levels of inflammatory factors (IL-6, TNF-α and MCP-1), LDH, ROS and MDA were significantly elevated in the HG group compared with controls. Significantly decreased SOD, CAT and GPx activities and significantly increased numbers of apoptotic cells were observed in the HG group compared with controls. The cells transfected with miR-145 mimics exhibited significantly decreased LDH, ROS and MDA levels, significantly increased antioxidant enzyme activities and significantly decreased apoptotic rates compared with controls, while the opposite results were observed in cells transfected with pcDNA3.1-ARF6. Moreover, co-transfection with miR-145 mimics and pcDNA3.1-ARF6 exacerbated the inflammatory response and OS injury in HG-induced cardiomyocytes compared with cells transfected with miR-145 mimics alone. Furthermore, miR-145 negatively targeted ARF6. miR-145 attenuated the HG-induced inflammatory response and OS injury in cardiomyocytes by negatively regulating ARF6, which may contribute to providing a theoretical basis for the treatment of diabetic cardiomyopathy.
The complete mitochondrial genome of Reticulitermes ovatilabrum (Isoptera: Rhinotermitidae)

Abstract The mitochondrial genome of the Reticulitermes ovatilabrum is 15,913 bp in length and encodes 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and a non-coding control region (D-loop). The percentage of A/T (65.59%) is much higher than that of C/G (34.41%). The phylogenetic tree revealed that R. ovatilabrum was closest to R. kanmonensi and R. periflaviceps. The mitochondrial genome of the R. ovatilabrum provides a resource for evolutional analysis within termites especially Reticulitermes.
New data on the mitochondrial genome of the winter crane fly (Diptera: Trichoceridae) with phylogenetic analysis

Abstract The family Trichoceridae is a small group in Nematocera (Diptera) with 157 known species in the world. In this study, we report a complete mitochondrial genome sequence of a Trichoceridae species, which is a circular molecule of 16,094 bp with 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a 1063 bp long non-coding region (control region). Eight gene overlaps ranging from 1 to 8 bp in length and 16 small non-coding intergenic spacers ranging from 1 to 77 bp in length are found in the mitochondrial genome. The canonical start codons (ATN) for invertebrate mitochondrial genomes are found in 11 PCGs, except for COI which uses TTG and ND5 uses GTG. Stop codons of 12 PCGs are invariably complete TAA and TAG, while COII ends with a single thymine stop codon. Twenty-two tRNAs ranges from 64 bp to 72 bp, and the rRNAs are determined to be 1326 bp in length for lrRNA and 788 bp in length for srRNA. Phylogenetic analysis reveals that the family Trichoceridae is a sister-group to the remaining Tipulomorpha, and the relationship between the other families is Pediciidae + (Limoniidae + (Cylindrotomidae + Tipulidae)).
A novel multimeric IL15/IL15Rα-Fc complex to enhance cancer immunotherapy

ABSTRACT The role of T cells in controlling human cancers is well known. Their success requires continued persistence in vivo and efficient trafficking to tumor sites, requirements shared by other effectors such as Natural Killer (NK) cells. To date, cytokine IL2 remains the only clinically approved cytokine therapy available to expand, maintain, and activate these effector lymphoid cells, but toxicities can be severe. Cytokine IL15 offers similar T cell proliferation and activation properties, but without the unwanted side-effects seen with IL2. Several IL15-cytokine fusion proteins have been developed to improve their in vivo function, typically exploiting the IL15Rα to complex with IL15, to extend serum half-life and increase affinity for IL15β receptor on immune cells. Here we describe a novel IL15 complex incorporating the full-length IL15Rα to complex with wild type IL15 to form spontaneous trimers of dimers (6 IL15 + 6 IL15Rα) during co-expression, resulting in a substantial increase in serum half-life and enhancement of in vivo cytokine effect on IgG or T cell engaging antibody-dependent cell-mediated cytotoxicities, when compared to alternative strategies.
Predictors of Health-Care Workers’ Unwillingness to Continue Working During the Peak of COVID-19 in Western Ethiopia: An Extended Parallel-Process Model Study

Purpose Willingness to work in disasters is context-specific and corresponds to the nature, magnitude, and threats posed by a particular public health emergency. None us is certain that our health professionals will continue to provide service should the COVID-19 pandemic crisis climb to its worst level. It was with this uncertainty in mind that this study was done to assess predictors of the unwillingness of health-care workers (HCWs) to continue providing their professional services during the climax of the COVID-19 crisis. Methods This was a facility-based descriptive cross-sectional study undertaken among 633 HCWsin western Ethiopia. Results Overall, 205 (32.4%) providers said that they would be unwilling to continue work if COVID-19 peaked. Of these, 176 (27.9%) respondents reported that they would stop going in to work before they were at greatest risk. Statistical analysis performed to predict HCWs unwillingness’ to continue work at peak COVID-19 showed male sex (AOR 11.4, 95% CI 8.32–12.6), younger age (AOR 25.3, 95% CI 4.61–40.67), lack of experience in handling similar pandemics (AOR 5.15, 95% CI 1.1–255), and low perceived level of hospital preparedness (AOR 2.05, 95% CI 0.80–5.21) were predictors of unwillingness. In accordance with the extended parallel-process model, higher threat perception (P≤0.001) and low efficacy perception (P≤0.040) were associated with unwillingness of the HCWs to continue working. Conclusion The proportion of HCWs unwilling to continue their job during COVID-19 is sufficient to affect efforts tof fight the pandemic. As the question of whether our HCWs must risk themselves to treat COVID-19 patients does not have a uniform answer, working on predictors of potential unwillingness is of paramount importance.
Association between Coalmine Dust and Mortality Risk of Lung Cancer: A Meta-Analysis

Background Evidence on the carcinogenicity of coalmine dust in occupational settings is still conflicting. Therefore, we conducted this research to evaluate the mortality risk of lung cancer for coalminers exposed to occupational dust when compared to population with no or low dust exposure. Methods Databases of PubMed and Chinese National Knowledge Infrastructure as well as reference lists were searched updated to September 18, 2020. The enrolled articles should report lung cancer mortality risk for coalminers exposed to occupational dust. Basic information was extracted such as the author and publication year, area and ethnicity, the type and estimates of outcome, duration of follow-up, and the study design. The checklists from Agency for Healthcare Research and Quality and the Newcastle-Ottawa Scale were used for the assessment of quality and bias risk for descriptive studies, cohort studies, and case control studies, respectively. The overall relative risks were calculated while Begg's and Egger's tests and sensitivity analysis were performed to explore potential heterogeneity sources. Metaregression and subgroup analyses were also conducted to give more detailed information for the correlation between dust exposure and lung cancer mortality. Results A total of 19 articles with 22 different studies (descriptive study, case control study, and cohort study) including 8909 observed deaths from 1964 to 2017 were enrolled with a significant heterogeneity (I2 = 95%, P < 0.001). The pooled relative risk of mortality from lung cancer was 1.16 (1.03-1.30) for coalminers. Results of metaregression analysis indicated that the high heterogeneity among these enrolled studies might be caused by the ethnicity differences (P = 0.011). Subgroup analysis also indicated that the pooled estimate for Asian population in China was 4.94 (3.95-6.17) with I2 = 39.3% and P = 0.192. All these results suggested that exposure to occupational dust would significantly increase the mortality risk of lung cancer, especially for Asian population in China, which should be measured and controlled more strictly. Discussion. This systematic review and meta-analysis provides high-quality evidence that exposure to occupational dust might increase the mortality risk of lung cancer, especially for Asian populations in China. The magnitude of this effect is of major public health importance in view of the ubiquitous existence of coalmining industry in China and even in the world. However, these pooled estimates should be interpreted cautiously because of the high heterogeneity among these publications. Other. This study was supported by the National Key Research and Development Program of China (Grant No. 2016YFC1302501).
Glomerulonephritis in Two Patients with SpA Treated with TNF-α Blockers and a Review of the Literature

Abstract Renal failure or acute/chronic kidney damage may present as a clinical manifestation of rheumatic diseases. In addition treatment with DMARDs or biologic drugs may induce nephrotoxicity. In this case-based review, we present two patients with SpA under anti-TNF-α treatment admitted to our hospital because of renal failure and proteinuria. We review previously published yet isolated cases of TNF-α blocker-induced glomerular disease in patients with SpA. Renal manifestations are occasionally seen in patients with ankylosing spondylitis and psoriatic arthritis with IgA nephropathy being the most common of them. Anti-TNF-α agents although reportedly used for the treatment of glomerular nephropathy as a disease manifestation, they have been considered responsible for provoking renal damage in some cases. A diagnostic approach for patients with SpA treated with anti-TNF-α agents presenting with renal manifestations is proposed herein.
Characterization and phylogenetic analysis of the complete chloroplast genome of Actinidia latifolia (Actinidiaceae)

Abstract The complete chloroplast (cp) genome of Actinidia latifolia was sequenced and assembled using Illumina pair-end sequencing data. The cp genome is 157,021 bp in length and comprises a large single copy (LSC) region of 88,557 bp and a small single copy (SSC) region of 21,562 bp separated by two inverted repeat (IR) regions of 23,451 bp. A total of 113 unique genes were identified, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Phylogenetic analysis based on cp genomes of 20 Actinidiaceae species revealed that A. latifolia was evolutionarily close to A. eriantha, A. styracifolia, and A. fulvicoma.
The complete chloroplast genome of Catalpa fargesii Bur. f. duclouxii (Dode) Gilmour

Abstract In this study, we reported and characterized the complete chloroplast genome sequence of Catalpa fargesii Bur. f. duclouxii (Dode) Gilmour. The chloroplast genome was determined to be 158,250 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 84,929 bp and 12,663 bp, respectively, which were separated by a pair of 30,329 bp inverted repeat (IR) regions. The genome is predicted to contain 121 genes, including 78 protein-coding genes, 35 tRNA genes, and 8 rRNA genes. The overall GC content of the genome is 38.1%. A phylogenetic tree reconstructed by 12 chloroplast genomes reveals that C. fargesii is mostly related to Catalpa. ovata and Catalpa. speciosa. This study identified the unique characteristics of the C. fargesii cp genome, which will provide a theoretical basis for species identification and biological research.
Complete chloroplast genome sequence and phylogenetic analysis of Chimonobambusa sichuanensis (Bambusoideae)

Abstract Chimonobambusa sichuanensis is an ornamental shrubby bamboo endemic to southern China. In this study, the complete chloroplast genome (cpDNA) sequence of Chimonobambusa sichuanensis was first reported. The cpDNA is 139,594 bp in length, including a small single-copy (SSC) region of 12,820 bp and a large single-copy (LSC) region of 83,196 bp, which were separated by a pair of inverted repeat (IR) regions of 21,789 bp. The genome contains 140 genes, consisting of 93 protein-coding genes, seven ribosomal RNA (rRNA) genes, and 40 transfer RNA (tRNA) genes. The phylogenetic analysis showed that C. sichuanensis is highly clustered in the Phyllostachys clade, sister to C. tumidissinoda.
LncRNA RP11-248E9.5 and RP11-456D7.1 are Valuable for the Diagnosis of Childhood Pneumonia

Background Pneumonia is a common infection of the lung parenchyma in children, and early and accurate diagnosis of childhood pneumonia (CP) is important for implementing appropriate preventive and treatment strategies. This study aimed to evaluate the diagnostic value of the combination of long non-coding RNA (lncRNA) RP11-248E9.5, RP11-456D7.1, c-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in CP. Patients and Methods A total of 50 healthy children (HC) and 100 CP patients were enrolled. The serum expression of RP11-248e9.5 and RP11-456d7.1 was detected by qRT-PCR. The white blood cell (WBC), hemoglobin (HB), platelet (PLT), neutrophil, and lymphocyte were analyzed by automated hematology analyzer. The serum levels of CRP and procalcitonin (PCT) were analyzed by automatic biochemical analyzer. The receiver operating characteristic (ROC) curves were applied to evaluate the diagnostic value in CP. Results The NLR and PLR, expression of RP11-248E9.5 and RP11-456D7.1, and serum levels of CRP and PCT were significantly higher in the CP group than those in the HC group. Both RP11-248E9.5 (AUC, 0.86; sensitivity, 84%; specificity, 78%) and RP11-456D7.1 (AUC, 0.89; sensitivity, 79%; specificity, 92%) exhibited certain diagnostic value in CP. The diagnostic values of PCT, CRP, NLR and PLR in CP were limited by low sensitivity (≤ 71%). The combination of multiple indicators improved the diagnostic value. The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR had the best diagnostic value in CP (AUC, 0.992; Sensitivity, 0.97; Specificity, 0.99). Conclusion The combination of RP11-248E9.5, RP11-456D7.1, CRP, NLR, and PLR was a potential diagnostic strategy for CP.
Reshaping experiential education within Qatar University's Health Programs during the COVID-19 pandemic

Health professions programs heavily depend on experiential learning to prepare learners for practice within the healthcare system. Learners acquire a significant proportion of patient care skills as they participate in experiential learning activities. As the coronavirus disease 2019 (COVID-19) pandemic disrupts education globally, educators have been challenged to reexamine existing teaching approaches to minimize the impact on experiential educational outcomes. This article describes how educators from the College of Pharmacy and College of Medicine at Qatar University utilized nontraditional teaching methods to ensure the continuation of experiential learning despite the disruption due to the pandemic.
The complete mitochondrial genome of Silurus grahami Regan, 1907 (Siluriformes: Siluridae), a native catfish in Fuxian Lake

Abstract In this study, the whole mitochondrial genome of Silurus grahami was reported to be 16,518 bp in length, including 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and one control region. The phylogenetic analysis based on 13 protein-coding genes showed that S. grahami was sister to clade of S. meridionalis and S. lanzhouensis. A total of 81 bases differences were identified in COI barcoding region, which could be used for species identification in catfish.
Sustained Delivery of SB-431542, a Type I TGF-β1 Receptor Inhibitor, to Prevent Arthrofibrosis.

Fibrosis of the knee is a common disorder resulting from an aberrant wound healing response and is characterized by extracellular matrix deposition, joint contraction, and scar tissue formation. The principal regulator of the fibrotic cascade is transforming growth factor beta-1 (TGF-β1), a factor that induces rapid proliferation and differentiation of resident fibroblasts. Here we demonstrate successful inhibition of TGF-β1-driven myofibroblastic differentiation in human fibroblast-like synoviocytes using a small molecule TGF-β1 receptor inhibitor, SB-431542. We also demonstrate successful encapsulation of SB-431542 in poly(lactide-co-glycolide) (PLGA) as a potential prophylactic treatment for arthrofibrosis and characterize drug release and bioactivity in a 3D collagen gel contraction assay. We assessed the effects of TGF-β1 and SB-431542 on cell proliferation and viability in monolayer cultures. Opposing dose-dependent trends were observed in cell proliferation, which increased in TGF-β1 treated cultures and decreased in SB-431542 treated cultures relative to control (p < 0.05). SB-431542 was not cytotoxic at the concentrations studied (0-50 μM) and inhibited TGF-β1-induced collagen gel contraction in a dose-dependent manner. Specifically, TGF-β1 treated gels contracted to 18 ± 1% of their initial surface area while gels treated with TGF-β1 and ≥10 μM SB-431542 showed no evidence of contraction (p < 0.0001). Upon removal of the compound, all gels contracted to control levels after 44 hours in culture, necessitating sustained delivery for prolonged inhibition. To this end, SB-431542 was encapsulated in PLGA microspheres (SBMS) that had an average diameter of 87.5 ± 24 μm and a loading capacity of 4.3 μg SB-431542 per mg SBMS. Functional assessment of SBMS revealed sustained inhibition of TGF-β1-induced gel contraction as well as hallmark features of myofibroblastic differentiation including α-smooth muscle actin expression and connective tissue growth factor production. These results suggest that SB-431542 may be used to counter TGF-β1 driven events in the fibrotic cascade in the knee cartilage.
Effect of oxygen plasma treatment of indium tin oxide for organic solar cell

We have investigated the contribution of oxygen ions and electrons in the oxygen plasma treatment of an indium tin oxide (ITO) electrode for organic solar cell (ITO/CuPc/C60/CuPc/Al). The cell characteristics were markedly improved by the treatments using positive oxygen ions and electrons. In the case of treatment by positive oxygen ions, short‐circuit photocurrent density increased markedly. The enhancement of photocurrent was explained in terms of a lowering of the total resistance between the CuPc layer and ITO due to an increase in the work function and highly oriented stacking in CuPc molecular configurations during the growth process. In the case of treatment by electrons, on the other hand, the fill factor showed a high value of about 0.5. However, further studies are needed for confirmation of this phenomenon. © 2006 Wiley Periodicals, Inc. Electr Eng Jpn, 154(4): 1–7, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20313