Miracle-dz/UniFAR-FTUs huggingface dataset:
Long non-coding RNA MEG3 regulates autophagy after cerebral ischemia/reperfusion injury

Severe cerebral ischemia/reperfusion injury has been shown to induce high-level autophagy and neuronal death. Therefore, it is extremely important to search for a target that inhibits autophagy activation. Long non-coding RNA MEG3 participates in autophagy. However, it remains unclear whether it can be targeted to regulate cerebral ischemia/reperfusion injury. Our results revealed that in oxygen and glucose deprivation/reoxygenation-treated HT22 cells, MEG3 expression was obviously upregulated, and autophagy was increased, while knockdown of MEG3 expression greatly reduced autophagy. Furthermore, MEG3 bound miR-181c-5p and inhibited its expression, while miR-181c-5p bound to autophagy-related gene ATG7 and inhibited its expression. Further experiments revealed that mir-181c-5p overexpression reversed the effect of MEG3 on autophagy and ATG7 expression in HT22 cells subjected to oxygen and glucose deprivation/reoxygenation. In vivo experiments revealed that MEG3 knockdown suppressed autophagy, infarct volume and behavioral deficits in cerebral ischemia/reperfusion mice. These findings suggest that MEG3 knockdown inhibited autophagy and alleviated cerebral ischemia/reperfusion injury through the miR-181c-5p/ATG7 signaling pathway. Therefore, MEG3 can be considered as an intervention target for the treatment of cerebral ischemia/reperfusion injury. This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Zhengzhou University, China (approval No. XF20190538) on January 4, 2019.
Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery

The mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery, is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral infarction were created by injecting fluorescent microspheres, 45–53 µm in diameter, into the common carotid artery. Six hours after modeling, fluorescent microspheres were observed directly through a fluorescence stereomicroscope, both on the brain surface and in brain sections. Changes in blood vessels, neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry. The microspheres were distributed mainly in the cerebral cortex, striatum and hippocampus ipsilateral to the side of injection. Microinfarcts were found in the brain regions where the fluorescent microspheres were present. Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia. These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts. This model is an effective, additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions. This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences (approval No. D2021-03-16-1) on March 16, 2021.
Job satisfaction among family medicine physicians in Saudi Arabia

Objective: Physicians are subject to chronic stressors, depression, and burnout due to long working hours, high requirements, and critical decision-making. [1],[2],[3],[4],[5] All those reasons contribute to the dissatisfaction of physicians. The dissatisfaction of physicians might lead to lower health-care quality. [6] Moreover, patient satisfaction is strongly affected by physician satisfaction. [7],[8] This study aims to measure job satisfaction among family medicine (FM) physicians in Saudi Arabia. Study Design: In this cross-sectional study, we recruited 265 FM physicians working in Saudi Arabia to participate in an online survey between October 2019 and January 2019. Results: Results showed that more than 50% of the respondents were very satisfied with their career choice (55.5%, n = 147). Non-Saudis who were satisfied or strongly satisfied were higher than those of Saudis (P = 0.035) and 2.45 times more likely to be dissatisfied compared to non-Saudi respondents. Respondents from the southern region were 81% less likely to be dissatisfied than respondents from the central region (OR = 0.19, P < 0.05). Conclusion: Family medicine physicians showed a high level of satisfaction with their career choice regardless of gender, age, sector public or private, marital status. This is promising for family medicine as a medical specialty. The future of health care in Saudi Arabia is driven toward general practice and primary care centers, which aligns with the future vision of Saudi Arabia 2030.
Early multicenter experience of a new balloon expandable MyVal transcatheter heart valve in dysfunctional stenosed right ventricular outflow tract conduits

Background : Transcatheter pulmonary valve implantation (TPVI) is a surgical alternative for correcting dysfunctional right ventricular outflow tract conduits in previously operated patients. MyVal transcatheter heart valve (THV) (Meril Life Sciences, India), a new transcatheter valve designed for aortic position has not been used for TPVI. Methods : Patients with stenosed dysfunctional conduits from the right ventricle to pulmonary artery (RV-PA) were prestented after initial computed tomography and balloon interrogation before the implantation of MyVal. Size of MyVal was chosen based on the final diameter of the prestent. Procedural details and post-TPVI follow-up were analyzed. Results : Seven patients aged 17–60 years (median 26 years) had stenosed RV-PA conduits implanted 5–17 years (median 9 years) ago for tetralogy of Fallot in three, following Ross procedure in two, repair of pulmonary stenosis, and following PA debanding in one patient each. Prestenting improved the conduit diameter from 9.3 ± 2.8 mm to 20.8 ± 1.1 mm and relieved the gradient from 87.3 ± 31.7 mmHg (50–137 mmHg) to 12.7 ± 6.4 mmHg (5–20 mmHg). A 23 mm MyVal was implanted in all the seven patients successfully; one patient needed an additional 24.5 mm MyVal valve in valve implantation for residual regurgitation. The mean fluoroscopic time and dose area product were 38.7 ± 25.3 min and 66.917 ± 39.211Gray. cm2, respectively. At a median follow-up duration of 16 months (10–22 months), all patients were asymptomatic receiving dual antiplatelet therapy with no PR and the gradient was 12.5 ± 5.8 mmHg on echocardiography. Although one patient needed an additional valve-in-valve implantation, there were no valve-related adverse events. Conclusions : Early experience of TPVI with MyVal THV in prestented conduits is encouraging with procedural success in all patients and acceptable mid-term outcomes.
Three-dimensional in vitro models of neuromuscular tissue

Skeletal muscle is a dynamic tissue in which homeostasis and function are guaranteed by a very defined three-dimensional organization of myofibers in respect to other non-muscular components, including the extracellular matrix and the nervous network. In particular, communication between myofibers and the nervous system is essential for the overall correct development and function of the skeletal muscle. A wide range of chronic, acute and genetic-based human pathologies that lead to the alteration of muscle function are associated with modified preservation of the fine interaction between motor neurons and myofibers at the neuromuscular junction. Recent advancements in the development of in vitro models for human skeletal muscle have shown that three-dimensionality and integration of multiple cell types are both key parameters required to unveil pathophysiological relevant phenotypes. Here, we describe recent achievement reached in skeletal muscle modeling which used biomaterials for the generation of three-dimensional constructs of myotubes integrated with motor neurons.
Managing systems evolving in space and time: four challenges for maintenance, evolution and composition of variants

Software companies need to provide a large set of features satisfying functional and non-functional requirements of diverse customers, thereby leading to variability in space. Feature location techniques have been proposed to support software maintenance and evolution in space. However, so far only one feature location technique also analyses the evolution in time of system variants, which is required for feature enhancements and bug fixing. Specifically, existing tools for managing a set of systems over time do not offer proper support for keeping track of feature revisions, updating existing variants, and creating new product configurations based on feature revisions. This paper presents four challenges concerning such capabilities for feature (revision) location and composition of new product configurations based on feature/s (revisions). We also provide a benchmark containing a ground truth and support for computing metrics. We hope that this will motivate researchers to provide and evaluate tool-supported approaches aiming at managing systems evolving in space and time. Further, we do not limit the evaluation of techniques to only this benchmark: we introduce and provide instructions on how to use a benchmark extractor for generating ground truth data for other systems. We expect that the feature (revision) location techniques maximize information retrieval in terms of precision, recall, and F-score, while keeping execution time and memory consumption low.
Cinétiques de dissolution des cristaux dans les silicates fondus - contexte des verres nucléaires

This work focuses on the dissolution kinetics of rare earth (RE) silicates (Ca2RE8(SiO4)6O2 where RE=Nd) with an apatite structure in a sodium-borosilicate glass melt. Dissolution kinetics were characterized by following the crystalline area fraction by image analysis as a function of time and temperature. Then, a model based on the generalized Johnson-MehlAvrami-Kolmogorov equation is proposed to fit the experimental data. This model enables to determine both the mechanism limiting the dissolution (i.e. the diffusion) and the activation energy of crystals dissolution in the studied glass system (496 kJ/mol). To support these results, the chemical profiles at the crystal/melt interfaces are measured by microprobe. The conclusions obtained by this last method are in agreement with the conclusions based on JMAK model. All these results allowed to confirm that the JMAK model is well suited to model crystals dissolution in silicate melts.
OPTH_A_317708 2481..2488

1Ophthalmology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt; 2Cabinet Ophtalmologie, Alger Centre, Algiers, Algeria Background: This study aimed to compare ocular surface parameter changes in active and inactive thyroid eye disease (TED) patients and controls. Methods: This is an observational clinical study that included 60 eyes divided into three groups following clinical activity score (CAS) assessment. The first group (Group A) comprised 20 eyes with active TED (CAS score ≥3/7), while the second group (Group B) comprised 20 eyes with inactive TED (CAS score <3/7) and the third group (Group C) comprised 20 eyes of controls without dry eye manifestations. The palpebral fissure height, degree of proptosis, degree of lagophthalmos, ocular surface disease index (OSDI), Schirmer test without anesthesia, corneal fluorescein staining (CFS), non-invasive tear break-up time (NITBUT), tear meniscus height (TMH), lipid layer thickness (LLT), meiboscore, meibomian gland dysfunction (MGD), and thyroid antibodies were assessed and data were compared between study participants. Results: The mean OSDI was 40.0 ±5.80 in Group A, which significantly differed from Group B with a mean of 26.5 ±5.10 (p=0.02). There were significant differences between the two groups regarding palpebral fissure height (p=0.02), amount of proptosis (p=0.008), and degree of lagophthalmos (p=0.001). Similarly, active TED patients had more decreased tear secretion than inactive TED patients (p=0.012). Moreover, active TED patients showed a significant increase in Meibomian gland loss areas in both upper and lower eyelids compared with inactive TED patients (p=0.001). Corneal fluorescein staining also revealed a statistically significant difference between the studied groups (p=0.0001). Conclusion: This study showed differences regarding ocular surface parameter changes between both active and inactive TED and compared to controls. Further studies are needed to confirm these results.
Evaluation of the Economic Profitability of Using Renewable Energy Sources in Agro-Industrial Companies

The study of the impact of the availability of renewable energy sources (RES) on the competitiveness and cost reduction opportunities is an actual objective for agro-industrial enterprises in the present time. The purpose of this paper was to develop a methodological approach based on a questionnaire survey, an integrated indicator of enterprise competitiveness, a model for assessing energy conservation costs, and regression analysis combined with a hierarchical synthesis of 20 Russian agro-industrial companies. The solution of the problem of nonlinear optimization of agro-industrial companies made it possible to determine the excess of the competitiveness criterion over the criterion of energy conservation cost reduction as a result of the use of RES. The conducted regression analysis showed a close relationship between the competitiveness of the companies under study and the availability of RES. Modeling and hierarchical synthesis of the study results confirmed that the use of RES in the activities of agro-industrial companies stimulates their competitive potential reflected by the competitiveness index and minimizes energy conservation costs. It was also found that there is an inverse regression relationship with a high degree of correlation between the provision of RES and the cost of energy-saving measures. Hence, it can be argued that a greater emphasis on the provision of Russian agro-industrial enterprises with RES can lead to their more sustainable and efficient development due to increased competitiveness and better cost reduction strategies.
AUGMENTED REALITY ASSET TRACKING USING HOLOLENS

Asset Tracking is an essential component of building management process. It involves creating and maintaining a database of detailed information of assets such as location, condition, brand, and type. This information can help building owners make informed decisions for cost-effective maintenance of building assets. Existing approaches to perform asset tracking require a manual process of measuring and recording the asset condition and location, which is labour-intensive and costly. The typical approach usually includes a human operator with pen and paper inspecting the site and manually recording the information about the asset. In this paper, we propose an augmented reality asset tracking system using HoloLens to reduce the manual labour involved in this process. The system can automatically detect the asset, record and update its related information by visual inspection. Assets are detected by feeding images captured by the HoloLens built-in camera to a pre-trained object detection network. Using a combination of various sensor readings from the HoloLens, the system can estimate the location of the asset using visual simultaneous localization and mapping (vSLAM). This information is then viewed and verified by the user using the augmented reality user interface. Upon the user confirmation, this information will be uploaded to a database. As a case study, we demonstrate a vending machine tracking system which is able to detect and localise the vending machines in an indoor environment and create a database of vending machine information. The system can detect vending machines with a mean average precision of 94.8% and a localization accuracy of 2.3 meters without pre-screening or
Epistatic Effects of Six Candidate Genes on Fatty Acid Composition in
 Korean Native Chicken

Fatty acid composition is an important economic trait that affects meat flavor. Several genes that influence fatty acid composition in meat have been investigated. In a previous study, we identified 51 significant SNP × SNP interactions (P≤0.05) between nine SNPs of six candidate genes (DEGS1, ELOVL6, FABP3, FABP4, FASN, and SCD) on meat fatty acid composition in Korean native chicken. This study further investigated the patterns of the SNP × SNP interactions to understand how they affect the fatty acid content in thigh and breast meat of Korean native chicken. The significant epistatic effects of SNP combinations showed various patterns for each fatty acid trait. The results of this study suggest that the respective additive effects of each SNP on polygenic traits, such as fatty acid composition, should be considered in combination with the epistatic effect of SNP combinations in animal breeding programs. The findings of this study have provided new genetic information for improving meat quality, especially the fatty acid composition, of Korean native chicken. (
An illustrated step-by-step protocol for investigating liverwort chromosomes

Cytogenetic studies in bryophytes have been limited by the difficulty of obtaining sufficient dividing nuclei and by the absence of modern protocols. the technical difficulties stem from the plants’ small size and lack of roots and pollen mother cells, the main sources of cells in division in vascular plants. In bryophytes instead, tiny sporophytes, antheridia, or phyllid meristems must be used to obtain meiotic or mitotic chromosome spreads. We here describe the preparation of such spreads from phyllids, antheridia, and sporophytes in several species of liverworts and compare available protocols with or without prefixation treatments. We also provide illustrated step-by-step instructions. the three prefixation agents (including colchicine) that we tested failed to improve synchronization of cell divisions. Young sporophytes were the best source of diploid synchronized cells, while antheridia were the best source of haploid cells. For meiotic nuclei, a short fixation of capsule tissue at the right developmental stage with 45% acetic acid sufficed to conserve the DNA for cytological investigations, while for mitotic nuclei, fixation in 3:1 ethanol/glacial acetic acid for a longer period (4–24 h) worked well.
The Efficiency of a Group Counseling Program Based on Psychodrama in Enhancing Self-Awareness and Reducing Tension Among Tenth Grade Students in Qalqilia City

This research was conducted through two studies, the purposes of the first study were to assess tension and self-awareness levels among the tenth-grade male and female students in Qalqilia city in Palestine, and to examine the impact of gender on tension and self-awareness. To achieve these goals a descriptive method was used, the sample consisted of (158) male and (152) female students age 16 years old. They were stratified randomly selected. This study found tension appears to be a broad problem in Palestine among respondents, and it is chronically existent with a high level. On the other hand, this study proved that self-awareness among Palestinian adolescents is low. No significant differences in tension and self-awareness due to gender were found. The objective of study 2 was, to examine the effect of psychodrama on the levels of tension and self-awareness of tenth-grade male students in Qalqilia city using the two-matching group pretest-posttest design. The experimental group consisted of (10) students and they received a psychodrama group counseling program, in order to reduce tension and enhance self-awareness. While the control group received nothing. Based on the results psychodrama is an effective tool to treat tension and enhance self-awareness. It is suggested that for future studies to be from different grades and both gender with larger sample size. It is also recommended to assess tension and self-awareness in different regions in Palestine.
Back-trap mottle: A review of mechanisms and possible solutions

Print mottle is a serious and common uneven printing defect that occurs when printing coated paper by the offset multicolor printing process. It is characterized by a non-uniform appearance in terms of brightness, gloss, or color density that appears mostly in solid printed areas. Back-trap print mottle and water-interference print mottle occur when the quality of the paper substrate, especially the coating layer quality, is not satisfactory. To cope with this quality problem of coated papers, the understanding of the offset printing process, the requirements of coated-paper quality, and the reasons for this problem should be addressed. In this review, the basic process of offset printing and the mechanisms of print mottles were explored, the importance of coating uniformity in both the coating structure and process was reviewed, and the approaches to cope with print mottle were introduced.
HASH TRICK BASED DEEP NEURAL NETWORK IMPLEMENTATION USING OPTIMIZED STOCHASTIC MULTIPLIER

—Deep neural networks (NN) have shown a significant promise in difficult tasks like image classification or speech recognition. Even well-optimized hardware implementations of digital NNs show significant power consumption. It is mainly due to non-uniform pipeline structures and inherent redundancy of numerous arithmetic operations that have to be performed to produce each single output vector. This paper provides a methodology for the design of well-optimized power-efficient NNs with a uniform structure suitable for hardware implementation with hash tricking . An error resilience analysis was performed in order to determine key constraints for the design of approximate multipliers that are employed in the resulting structure of NN. By means of a search based approximation method, approximate multipliers showing desired tradeoffs between the accuracy and implementation cost were created. Significant improvement in area efficiency was obtained in both cases with respect to regular NNs.
STATE-BUSINESS RELATIONS FROM THE PERSPECTIVE OF THE COMPANIES' PREPAREDNESS FOR THE CHANGES RELATED TO INDUSTRY 4.0: A CASE OF THE CZECH REPUBLIC

The purpose of this study is to investigate the expectations of the management of different industries in the Czech Republic related to governmental support for their implementation of Industry 4.0 and their preparedness for the challenges associated with these technological changes. Mixed research, specifically exploratory sequential design, was used. The findings of qualitative data analyses from in-depth semi-structured interviews (n=41) with representatives of different industry sectors formed subsequent quantitative data collection (N=146, the board of management members representing various industries) through survey items corresponding with formalized research questions. The findings revealed a strong group of companies already implementing Industry 4.0 elements (85% of respondents) driven by companies with 250+ employees. As perceived by the management, the critical threat related to the workforce is a lack of skilled labour. Managers tend to prefer a transparent business environment without state interventions or interventions targeted to strategic state industries, preferably to the spheres of education, research & development, energy sector, sustainable agriculture, and healthcare. The results indicate that the easier administration and education reform are among the companies' boldest expectations from the government, among general conditions for current business. Implications for the Central European audience: Central European companies face challenges related to implementing the Industry 4.0 elements. Therefore, the findings that aim to contribute to the debate addressing administration simplification, business environment transparency, and reform of the education system are considered relevant for the CE audience.