Miracle-dz/UniFAR-FTUs huggingface dataset:
Valentine Leukocidin In Vivo - Panton and Lung Inflammation to Staphylococcal TLR 2 and CD14 Mediate Innate Immunity

The pore-forming toxin Panton–Valentine leukocidin (PVL) is carried by community-acquired methicillin-resistant Staphylococcus aureus and associated with necrotizing pneumonia together with poor prognosis of infected patients. Although the cell-death– inducing properties of PVL have previously been examined, the pulmonary immune response to PVL is largely unknown. Using an unbiased transcriptional proﬁling approach, we show that PVL induces only 29 genes in mouse alveolar macrophages, which are associated with TLR signaling. Further studies indicate that PVL directly binds to TLR2 and induces immune responses via NF- k B in a TLR2, CD14, MyD88, IL-1R–associated kinase 1, and TNFR-associated factor 6-dependent manner. PVL-mediated inﬂammation is independent of pore formation but strongly depends on the LukS subunit and is suppressed in CD14/TLR2 2 / 2 cells. In vivo PVL or LukS induced a robust inﬂammatory response in lungs, which was diminished in CD14/TLR2 2 / 2 mice. These results highlight the proinﬂammatory properties of PVL and identify CD14/TLR2 as an essential receptor complex for PVL-induced lung inﬂammation. The Journal of Immunology , 2011, 186: 1608–1617.
Contextualization of the Trialability Concept in the SaaS Model for Mobile and Ubiquitous Environment Deployed in Public Cloud Possibilities, limitations, mitigations, cost and, measures for mobile SaaS (free trial) potential adopters

—In this research a generic commercial Software as a Service (SaaS) product offered in the mobile marketplace and deployed in a public cloud is analyzed in order to: (i) identify the possibilities and limitations of the “free to try” version in cloud computing environment; (ii) highlight the cost of the free trial; (iii) determine the correspondence between the “free to try” version and the one available after an onerous subscription contract; (iv) make reference to a series of metrics for measuring the intention to use the application, its effective and real use of available resources. The paper aims to facilitate an educated informed adoption (or rejection) with respect to a mobile SaaS commercial product deployed in public cloud, during the free trialability period, evaluating the SaaS characteristics and functionalities jointly with some perceptions and measures which could arise from its active use, inspection and consideration.
RAPD-PCR Based Biomarker Study in Fish Species (Family: Cyprinidae ) of Madhya Pradesh, India

Random Amplified Polymorphic DNA (RAPD) analysis was conducted for molecular identification of most commonly occurring fish species in Central India. In the study, male and female species of Labeo rohita, Catla catla and Cirrhina mrigala (family: Cyprinidae ) were randomly collected from various locations of central Indian region. For polymerase chain reaction, ten decanucleotide primers (RAn primer series) were used. On the basis of interpretability, simplicity and reproducibility, six random primers RAn 1 (GATGACCGCC), RAn 3 (GGCACGTAAC), RAn 4 (GGCATGACCT), RAn 5 (GGGTAACGCC), RAn 9 (GTGCCAAATG) and RAn11 (GTGCCCGTTA) were considered for genetic differentiation and band analysis. We observed varied size of amplified products depending upon the sequence of random primers and genotypes used. A total of 55 discrete amplified products were obtained (size 200 to 2000 bp approximately). Out of 55 products, 29 were species specific markers indicating high level polymorphism among species. The highest and lowest number of RAPD bands detected for primers RAn 5 and RAn 9 was 12 and 6 respectively. 16 bands were most relevant as found common in all three species of family: Cyprinidae . Highest monomorphism observed for C. catla and C. mrigala (5 bands) and lowest for L. rohita & C. mrigala (1 band). Similar band patterns were obtained for both male and female of all three fish species showing 100 percent sexual genetic similarity. The diverse nature of DNA bands indicated the genetic distance between fish species and presence of common bands attributed to an evolutionary relationship. Pattern of species specific unique bands observed might be useful tools for molecular identification. Our study re-establishes the importance of RAPD-PCR technique for molecular taxonomy studies in diverse fish species.
Impregnation of Wood with Waste Engine Oil to Increase Water ‐ and

Impregnation is a common method of protecting wood from external influences. This study proposes the use of spent engine oil as an impregnating composition for modifying birch wood to make it resistant to biological degradation and water. The indicators of water resistance and dimensional stability of wood such as wetting contact angle, thermogravimetric analysis, Fou‐ rier transform infrared spectroscopy (FTIR), and biodegradation tests have been determined. It has been found that treatment with spent engine oil significantly increases the dimensional stability (56.8% and 45.7% in tangential and radial directions) and water‐resistant indicators of wood. Ther‐ mogravimetric analysis has showed that the curves for the impregnated specimens were different from the control group and had two sharp peaks at 302 and 357 °C. However, FTIR indicated that no clear chemical reactions occur between spent engine oil and wood. A study on wood resistance to biological degradation has showed a significant increase in resistance against brown rot (Poria placenta fungi) in the treated specimens, in contrast to the control group. Thus, impregnation of wood with spent engine oil makes it possible to increase wood resistance to water and biological degradation.
Semi-automatic Registration of Mammographic Images for Breast Cancer Progression Assessment

Breast cancer is the most common cancer among women. Despite significant advances in the medical field and its related technologies, cancer remains one of the biggest problems in medical science. Point common in all cancers need to do surgery and remove the tumor in advanced cases. Unfortunately, the surgery is not successful in some cases and later re-released in the body. Sometimes changing in the patient's tissues hides from human sight. One of the problems of comparing images taken at different times is change in imaging condition that causes distortion in the image and the difference between the textures in the image. To solve this problem and reduce the error, in this paper a semi-automatic method is presented for mammographic images registration. In this method using feature extraction of a mammogram such as brightness of the image, transformation distance, and also information that the user presents, can obtain the actual relationship between images and determines the differences between the images with less error. The proposed method will run on a sample of 27 mammograms from the Parto Teb Azma Imaging Center. We will show that in addition to maximizing similarities and minimizing differences between two images, the second image retains its overall shape. Generally correlation coefficient of mammograms after image registration in the semi-automatic method increase to 0.2670 and the difference squared in the semi-automatic method will decrease to 0.0968.
E cacy and safety of jakinibs in rheumatoid arthritis: A systematic review and meta-analysis

Yufeng Yin First A liated Hospital of Soochow University https://orcid.org/0000-0001-8840-3397 Mengru Liu Shanghai Jiao Tong University Medical School A liated Ruijin Hospital Xin Chang First A liated Hospital of Soochow University Michun He First A liated Hospital of Soochow University Mingjun Wang First A liated Hospital of Soochow University Jian Wu (  njwujian@163.com ) First A liated Hospital of Soochow University
ThetypeIVsecretionsystema ¡ ectstheexpressionofOmp 25 / Omp 31 and theoutermembraneproperties ofBrucellamelitensis

The type IV secretion system (T4SS) contributes to Brucella intracellular survival through its effector proteins. Comparative proteomic analysis showed that intracellular survival proteins are expressed differentially in a virB mutant. Interestingly, several outer membrane proteins (OMPs) are also differentially expressed, implying that T4SS might affect the OM properties of Brucella. To further evaluate the impact of T4SS on OM, in the present study, the OM proteomes were isolated and compared. Many more products of OMPs, particularly different products of the Omp25/Omp31 family, were found to be altered in the virB mutant. The transcription profiles of Omp25/Omp31 were different from those of their protein products, implying their regulation by virB at both transcriptional and post-transcriptional levels. The virB mutant aggregates at a high cell density and produces exopolysaccharide, a phenotype resembling that of the vjbR mutant. The virB mutant showed increased sensitivity to polymyxin B and decreased survival under oxidative, high-salt and high-osmolarity stresses, indicating drastic membrane alterations. These results indicated that in addition to being an effector protein secretion system, T4SS affects OM properties that might be important for the adaptation of Brucella to both in vitro and in vivo hostile environments.
Collisional excitation of water in warm astrophysical media - I. Rate coefficients for rovibrationally excited states

Context: The interpretation of water line emission from infrared and submillimetre observations requires a detailed knowledge of collisional rate coefficients over a wide range of levels and temperatures. Aims: We attempt to determine rotational and rovibrational rate coefficients for H2O colliding with both H2 and electrons in warm, molecular gas. Methods: Pure rotational rates are derived by extrapolating published data using a new method partly based on the information (phase space) theory of Levine and co-workers. Ro-vibrational rates are obtained using vibrational relaxation data available in the literature and by assuming a complete decoupling of rotation and vibration. Results: Rate coefficients were obtained for the lowest 824 ro-vibrational levels of H2O in the temperature range 200-5000 K. Our data is expected to be accurate to within a factor of ~5 for the highest rates (? 10-11 cm3 s-1). Smaller rates, including the ro-vibrational ones, should be generally accurate to within an order of magnitude. As a first application of this data, we show that vibrationally excited water emission observed in evolved stars is expected to be at least partly excited by means of collisions. Tables A.1-A.4 are only available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/cgi-bin/qcat? J/A+A/492/257
Pulsed Power Radio Frequency System for the European XFEL at DESY

The pulsed power radio frequency system of the European XFEL under construction at present at DESY in Hamburg, Germany, consists of 27 pulsed power RF stations. It provides RF power at 1.3GHz for the superconducting cavities of the XFEL main linear accelerator, the cavities of the injector and the RF gun. Each station provides RF pulses up to 10MW, 1.37ms pulse duration and 10Hz repetition rate. It consists of a 120kV pulse generating system, a pulsed power 10MW RF source, a RF distribution system and a number of additional components for control and protection. In this paper an introduction to the XFEL project will be given. The design and status of the pulsed power RF system and its components will be described.
13 C Labeling Reveals Chloroplastic and Extrachloroplastic Pools of Dimethylallyl Pyrophosphate and Their Contribution to Isoprene Formation 1

Isoprene emitted from plants is made in chloroplasts from dimethylallyl pyrophosphate (DMAPP). Leaves of Populus nigra and Phragmites australis exposed to 13 CO 2 for 15 min emitted isoprene that was about 90% 13 C, but DMAPP isolated from those leaves was only 28% and 36% 13 C, respectively. The labeled DMAPP is likely to represent chloroplastic DMAPP contributing to isoprene emission. A substantial 13 C labeling was also found in both emission and DMAPP pool of low-emitting, young leaves of Phragmites. This conﬁrms that low emission of young leaves is not caused by absence of chloroplastic DMAPP but rather by enzyme characteristics. A very low 13 C labeling was found in the DMAPP pool and in the residual isoprene emission of leaves previously fed with fosmidomycin to inhibit isoprene formation. This shows that fosmidomycin is a very effective inhibitor of the chloroplastic biosynthetic pathway of isoprene synthesis, that the residual isoprene is formed from extra-chloroplastic sources, and that chloroplastic and extrachloroplastic pathways are not cross-linked, at least following inhibition of the chloro- plastic pathway. Reﬁxation of unlabeled respiratory CO 2 in the light may explain incomplete labeling of isoprene emission, as we found a good association between these two parameters.
Asymptotic allometry and transition to the canopy in <i><fc>A</fc>bies balsamea</i>

1. There is a lack of consensus in theoretical and empirical literature on whether height–diameter (H:D) relationships of canopy trees are asymptotic. 2. To investigate H:D allometry, particularly in the transition to the canopy, we focused on shadetolerant Abies balsamea, across steep physical gradients associated with elevation, and correlated biotically generated gradients of stem density, canopy height and canopy species composition. 3. We addressed these questions: A. What is the relation between H:D allometric form and emergence into the canopy? B. Is H:D allometry asymptotic? C. What aspects of the H:D relationship are most sensitive to environmental conditions? Using maximum likelihood, we compared the performance of alternative H:D forms (linear, power function, non-sigmoid asymptotic and sigmoid asymptotic), incorporating elevation and local canopy height as covariates. 4. Abies balsamea H:D allometric form was clearly sigmoid asymptotic, by a wide AIC margin, across all gradients, to an asymptote slightly above canopy height. The commonly used power function had essentially no empirical support (DAIC >> 10). 5. Canopy height decreased with elevation, but with much variation, so elevation was a poor predictor of the asymptote. Across environmental gradients, H:D allometries overlapped for trees far below the canopy, but diverged strongly for trees approaching different canopy heights. 6. Changes in resource allocation and modification of growth form that could generate asymptotic H:D allometries are consistent with known physical forces and diverse research findings in tree developmental biology and physiology. 7. Synthesis. The H:D asymptote and its tight relation to canopy height, unapparent in aggregated H:D data, became clear only when canopy height and elevation were included explicitly in allometric models. Asymptotic H:D allometries may be the norm, though often undetected, in unmanaged forests without recent catastrophic disturbance. Appropriate asymptotic H:D models may reduce error in forest carbon and biomass estimation and promote theoretical and empirical integration in the ecology and evolution of tree allometry.
Speech Enhancement using the Wave-U-Net with Spectral Losses

Speech enhancement and source separation are related tasks that aim to extract and/or improve a signal of interest from a recording that may involve sounds from various sources, reverberation, and/or degradation of capture quality. Taking into account that the Wave-U-Net is an end-to-end deep learning architecture that has obtained relevant results for the source separation task operating in the time domain, this thesis studies the performance of this architecture for the speech enhancement task in terms of denoising, dereverberation, decoloration, and bandwidth extension. The experiments were conducted using a combination of a noisy version of the Voice Bank Corpus (VCTK) and the Device and Produced Speech dataset (DAPS). In addition to the original framework, variations inspired by relevant deep learning networks for speech enhancement were explored here, of which losses with spectral components presented the most favorable eﬀects for the improvement of low-quality speech signals. Also, the concatenation of the input audio with a noise vector in the network was shown to generate more coherent high-frequency content in the output signal.
ReviewTranslational control of Xenopus oocyte meiosis: toward the genomic eraFerdinand Meneau

The study of oocytes has made enormous contributions to the understanding of the G2/M transition. The complementarity of investigations carried out on various model organisms has led to the identification of the M-phase promoting factor (MPF) and to unravel the basis of cell cycle regulation. Thanks to the power of biochemical approaches offered by frog oocytes, this model has allowed to identify the core signaling components involved in the regulation of M-phase. A central emerging layer of regulation of cell division regards protein translation. Oocytes are a unique model to tackle this question as they accumulate large quantities of dormant mRNAs to be used during meiosis resumption and progression, as well as the cell divisions during early embryogenesis. Since these events occur in the absence of transcription, they require cascades of successive unmasking, translation, and discarding of these mRNAs, implying a fine regulation of the timing of specific translation. In the last years, the Xenopus genome has been sequenced and annotated, enabling the development of omics techniques in this model and starting its transition into the genomic era. This review has critically described how the different phases of meiosis are orchestrated by changes in gene expression. The physiological states of the oocyte have been described together with the molecular mechanisms that control the critical transitions during meiosis progression, highlighting the connection between translation control and meiosis dynamics.
Understanding Student Retention in Engineering

The Academy of Engineering Success (AcES) program employs known best practices to support engineering students with the goal of retaining them through graduation and diversifying the engineering workforce. The AcES program started in 2012 and has been supported by NSF S-STEM award number DUE-1644119 since 2016. Cohorts from 2016, 2017, 2018, and 2019 consist of 12, 20, 22, and 17 students, respectively. Twenty-one renewable S-STEM supported scholarships have been awarded to students since 2016. AcES students participate in a one-week pre-fall bridge experience, a common fall professional development course, and a course emphasizing the role of engineers in societal development in the spring semester. Starting in the bridge experience and continuing until graduation, students participate in curricular and co-curricular activities with the goals of: (1) fostering feelings of belonging in engineering and institutional inclusion, (2) encouraging professional development, and (3) supporting academic achievement and student success. These goals are achieved by providing: (1) opportunities for interaction between students and peers, faculty, and industry mentors; (2) major and career exploration opportunities; and (3) academic support and student success education in areas such as time management and study skills. AcES students participate in the GRIT, LAESE, and MSLQ surveys, as well as in focus groups and one-on-one interviews at the start and end of each fall semester and at the end of the spring semester. The surveys provide a quantitative measure of students’ GRIT, general self-efficacy, engineering self-efficacy, test anxiety, math outcome efficacy, intrinsic value of learning, inclusion, career expectations, and coping efficacy. Qualitative data from the focus group and individual interview responses are used to provide insight into the quantitative survey results. Surprisingly, a previous analysis of the 2017 cohort survey responses revealed that students who left engineering had higher baseline values of GRIT, career expectations, engineering self-efficacy, and math outcome efficacy than those students who retained. Hence, the 2018 cohort survey responses were analyzed in relation to retention and are presented along with qualitative results to provide a holistic understanding of student retention. Results from both the 2017 and 2018 cohorts are presented and discussed in the paper and poster.
DEV103408 1..11

Through intercellular signalling, the somatic compartment of the foetal testis is able to program primordial germ cells to undergo spermatogenesis. Fibroblast growth factor 9 and severalmembers of the transforming growth factor β superfamily are involved in this process in the foetal testis, counteracting the induction of meiosis by retinoic acid and activating germinal mitotic arrest. Here, using in vitro and in vivo approaches, we show that prostaglandin D2 (PGD2), which is produced through both L-Pgds and H-Pgds enzymatic activities in the somatic and germ cell compartments of the foetal testis, plays a role in mitotic arrest in male germ cells by activating the expression and nuclear localization of the CDK inhibitor p21 and by repressing pluripotency markers. We show that PGD2 acts through its Dp2 receptor, at least in part through direct effects in germ cells, and contributes to the proper differentiation of male germcells through the upregulation of themaster geneNanos2. Our data identify PGD2 signalling as an early pathway that acts in both paracrine and autocrine manners, and contributes to the differentiation of germ cells in the foetal testis.
The Effect of Technological Innovation On Co2 Emissions in OECD Countries: Using Panel Regression

In the last century, energy consumption has grown in parallel with population growth and the extensive use of technology. While increased energy consumption has major environmental consequences due to carbon dioxide (CO2) emissions, it has also accelerated the search for renewable energy. In this study, we investigated the effect of technological innovations and renewable energy on carbon dioxide emissions. For this purpose, we examined the number of patents and the effects of renewable energy use on the environment of 37 Organization for Economic Co-operation and Development (OECD) countries, which constitute the world's largest economy, between 1990 and 2019, using the panel cointegration method. The empirical results show that the increase in the number of patents has a significant effect on CO2 emissions, while investment and GDP have a relatively small effect. It was concluded that renewable energy and export trade variables reduce CO2 emissions.
Antiantibodies to Cardiac Troponin Associate with Higher Initial Concentrations and Longer Release of Troponin I in Acute Coronary Syndrome Patients

BACKGROUND: Cardiac troponin (cTn) is an established marker of myocardial infarction. Pronounced heterogeneity and the minute amounts released into the circulation constitute significant challenges for cTn detection. Recently, autoantibody formation to cTn was shown to be common and to interfere with immunoassay performance. In this study, we investigated cTn autoantibodies and cardiac troponin I (cTnI) in acute coronary syndrome (ACS) patients over a 1-year period after the index event.