Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of N-terminal Myc Ubiquitylation and the Novel Oncogene CUL7

Myc is an oncogene that is commonly deregulated in human cancers. Mechanistic studies reveal that Myc is a transcription factor that interacts with a protein partner called Max. Heterodimerization and the formation of the Myc:Max complex enables Myc:Max to bind to the E-box and subsequently regulate the activation and repression of Myc target genes. Since regulation of its target genes are essential for Myc to drive transformation, the Myc and Max interaction has been targeted in mouse model studies to determine whether the oncogenic activity of Myc can be inhibited. Surprisingly, these studies reveal that targeting Myc is not only possible but a powerful way to suppress tumour growth. Since a better understanding of how Myc carries out its biological functions makes the possibility of targeting Myc a reality, it is essential to investigate and study the mechanisms of how Myc promotes tumourigenesis. In the first part of this thesis, we investigate the idea that the N-terminal end of Myc may be post-translationally modified and this modification may dictate Myc activities. Indeed we report here that the N-terminal end of Myc can be ubiquitylated as well as acetylated, and that the loss of these modifications results in a decrease in Myc activities. Furthermore, we characterize Mdm2 as a potential E3 ubiquitin ligase that may ubiquitylate the N-terminal end of Myc. In the second part of this thesis, we investigate CUL7 as a novel oncogene that may inhibit Myc-potentiated apoptosis and cooperate with Myc in transformation. Indeed, CUL7 is a novel p53 interacting protein that inhibits Myc potentiated apoptosis through the inhibition of p53. We have also characterized CUL7 to be overexpressed in primary human lung cancers, and a higher level of CUL7 expression associates with short-term survival of lung cancer patients. Through the better understanding of the enzymes that post-translationally modify the N-terminal end of Myc and proteins, such as CUL7, that can cooperate with Myc to drive tumourigenesis, we may begin to devise ways to target and control deregulated Myc in cancer cells. %%%%PhD
Discovery, Characterization, and Linkage Mapping of Simple Sequence Repeat Markers In Hazelnut

From the genome sequence of hazelnut (Corylus avellana), 192 new polymorphic simple sequence repeat (SSR) markers were developed, characterized, and used to investigate genetic diversity in 50 accessions. Next-generation sequencing allows inexpensive sequencing of plant genomes and transcriptomes, and efficient development of polymorphic SSR markers, also known as microsatellite markers, at low cost. A search of the genome sequence of ‘Jefferson’ hazelnut identified 9094 fragments with long repeat motifs of 4, 5, or 6 base pairs (bp), from which polymorphic SSR markers were developed. The repeat regions in the ‘Jefferson’ genome were used as references to which genomic sequence reads of seven additional cultivars were aligned in silico. Visual inspection for variation in repeat number among the aligned reads identified 246 as polymorphic, for which primer pairs were designed. Polymerase chain reaction (PCR) amplification followed by agarose gel separation indicated polymorphism at 195 loci, for which fluorescent forward primers were used to amplify the DNA of 50 hazelnut accessions. Amplicons were post-PCR multiplexed for capillary electrophoresis, and allele sizes were determined for 50 accessions. After eliminating three, 192 were confirmed as polymorphic, and 169 showed only one or two alleles in each of the 50 cultivars, as expected in a diploid. At these 169 SSRs, a total of 843 alleles were found, for an average of 4.99 and a range of 2 to 17 alleles per locus. The mean observed heterozygosity, expected heterozygosity, polymorphism information content, and the frequency of null alleles were 0.51, 0.53, 0.47, and 0.03, respectively. An additional 25 primer pairs produced more than two bands in some accessions with an average of 6.8 alleles. The UPGMA dendrogram revealed a wide genetic diversity and clustered the 50 accessions according to their geographic origin. Of the new SSRs, 132 loci were placed on the linkage map. These new markers will be useful for diversity and parentage studies, cultivar fingerprinting, marker-assisted selection, and aligning the linkage map with scaffolds of the genome sequence.
The Smile is The New Like: Controlling Music with Facial Expressions to Minimize Driver Distraction

The control of user interfaces while driving is a textbook example for driver distraction. Modern in-car interfaces are growing in complexity and visual demand, yet they need to stay simple enough to handle while driving. One common approach to solve this problem are multimodal interfaces, incorporating e.g. touch, speech, and mid-air gestures for the control of distinct features. This allows for an optimization of used cognitive resources and can relieve the driver of potential overload. We introduce a novel modality for in-car interaction: our system allows drivers to use facial expressions to control a music player. The results of a user study show that both implicit emotion recognition and explicit facial expressions are applicable for music control in cars. Subconscious emotion recognition could decrease distraction, while explicit expressions can be used as an alternative input modality. A simple smiling gesture showed good potential, e.g. to save favorite songs.
Characterization of antibiotic resistance phenotypes and resistance genes in Enterococcus spp. isolated from cheeses

Strains of Enterococcus spp. isolated from a collection of 123 artisanal and industrial cheese samples were studied for the phenotypic and genotypic assessment of antibiotic resistance. A total of 226 isolates included 119 E. faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29 E. faecalis (12.83%) and 1 E. gallinarum (0.44%). Out of 61 tested strains, 15 (24.59%) strains exhibited resistance to one or more tested antibiotics, as determined by the disc diffusion method. The resistance phenotypes were as follows: gentamicin (45.45%), tetracycline (31.82%), erythromycin (9.09%), vancomycin (9.09%) and penicillin (4.55%). The presence of tetracycline and erythromycin resistance genes [tet(M), tet(L) and erm(B), respectively] and integrase gene (int), associated with Tn916-1545 transposon family, was detected by PCR procedures. The tet(M) gene was determined in all 7 tested strains, but none of the analyzed strains harbored tet(L) determinant. The erm(B) gene was not detected in 9 strains characterized by phenotypic resistance to erythromycin. All 16 strains were positive for the presence of the int gene. The presented results show the presence of antibiotic resistance genes and the transposon integrase gene associated with transferable resistance in enterococci, indicating a potential for gene transfer through the food chain.
From Actor-Network Theory to Political Economy

While originally deployed within the sociology of scientific knowledge, actornetwork theory (ANT) has achieved some prominence across the social sciences and attracted a dedicated following. Its breach with previous methodologies for social theory borders on the anarchic as the structural and systemic are rejected as legitimate objects of study. In their place, we are offered a middlerange theory in which the extremes of macro and micro have been ostensibly excised. The macro does not exist other than in the minds of deluded structuralists, yet the micro (actor in particular) must be located (in a network) and vice versa.
Thermal preference, thermal tolerance and the thermal dependence of digestive performance in two Phrynocephalus lizards (Agamidae), with a review of species studied

We reported data on thermal preference, thermal tolerance and the thermal dependence of digestive performance for two Phrynocephalus lizards (P. frontalis and P. versicolor), and compared data among lizards so far studied worldwide. Mean values for selected body temperature (Tsel) and critical thermal maximum (CTMax) were greater in P. versicolor, whereas mean values for critical thermal minimum (CTMin) did not differ between the two species. The two lizards differed in food intake, but not in food passage time, apparent digestive coefficient (ADC) and assimilation efficiency (AE), across the experimental tem- peratures. Four general conclusions can be drawn from published data. Firstly, thermal preference and thermal tolerance differ among lizards differing in distribution, temporal activity pattern and habitat use. Lizards in thermally more variable regions are better able to tolerate low and high temperatures. Diurnal lizards generally select higher body temperatures than nocturnal lizards, and lizards using habitats with direct sun exposure generally selected higher body temperatures and are better able to tolerate high temperatures. Secondly, CTMax is positively correlated with Tsel. Lizards more likely exposed to extremely high temperatures while active select higher body temperatures than those using shaded habitats. Thirdly, the effects of body temperature on food intake, food passage time, ADC and AE differ among lizards, but it seems to be common among lizards that ADC and AE are less thermally sensitive than food intake and food passage time. Lastly, ADC is dependent on the type of food ingested, with insec- tivorous lizards digesting food more efficiently than herbivorous lizards (Current Zoology 57 (6): 684-700, 2011).
A New Frequency for Offshore Wind-farm Based on Component Loss Calculation

Offshore wind power plants are gaining importance in recent years, as there is adequate space available for its installation, high wind speed, no restriction on the size of turbine blades (no transportation and construction problem) and blades can be allowed to rotate at higher speed without any noise constraint, thereby increasing the rated power. However, the existing offshore wind farms face greater cost related challenges than those of onshore plants. The integration of offshore wind farm with onshore power grid is a complex issue. Feasible solutions for power transmission through cables from offshore wind farms to onshore are HVAC, line commutated HVDC and VSC-HVDC. This paper analyses the various schemes for integration of offshore wind farm with onshore power grid and suggests that LFAC with submarine cable operating at 0.7 Hz is an optimal choice in obtaining better performances.
More Adverts or More eWOM's

The increase use of internet as source of information by consumers has raised concern among firms as well as practitioners, regarding the effectiveness of such information. Consumers have become savvier using the internet to gather information about products and/or services; trust in traditional advertisements has decrease and Marketers are particularly interested in better understanding electronic wordof-mouth (eWOM) because traditional forms of communication “advertising” appear to be losing effectiveness [1]. Subsequently, majority of e-Shoppers perceive opinions posted online more trustworthy than brand communities [2]. Hypermedia has created many opportunities for eWOM communication in the field of marketing; as information can be readily accessed online. According to the New York Times 65% of new business comes from referrals; indicating that most customers are gained through referrals and not advertisement.
Detection of Influential Communities and Members in a Community Using Graph Mining Techniques

Graphs have been widely used to represent objects and object connections in applications Mining influence relationships from graphs provides information on how graph objects influence each others. It defines influence aspects and influence degrees to describe object connections in graph. Influence aspect to denote the area in which one object influences another object, and use the influence degree to denote how strong the influence is. In this new guidelines are propose to study interaction networks, where one considers not only the underlying topology of the social network, but also the exact time instances that nodes interact. In an interaction network an edge is associated with a time stamp, and multiple edges may occur for the same pair of nodes. Existing techniques focus on inferring either the influence types from graphs or the overall influence degrees. In this paper, P arallel instance-merging based method used to extract influence aspects from the context of object connections, then introduce two generative models, Observed Aspect Influence Model and Latent Aspect Influence Model. Both parallel and non parallel Gibbs sampling approach to learn the model parameter and latent states that control the generative process of LAIM and OAIM and to increase accuracy Prefix calculation Algorithm is used parallel with Gibbs sampling. To show the effectiveness and efficiency of our methods we use data sets.
Illusory gloss on Lambertian surfaces Perceptual Intelligence

It has recently been shown that an increase of the relief height of a glossy surface positively correlates with the perceived level of gloss (Y.-H. Ho, M. S. Landy, & L. T. Maloney, 2008). In the study presented here we investigated whether this relation could be explained by the finding that glossiness perception correlates with the skewness of the luminance histogram (I. Motoyoshi, S. Nishida, L. Sharan, & E. H. Adelson, 2007). First, we formally derived a general relation between the depth range of a Lambertian surface, the illumination direction and the associated image intensity transformation. From this intensity transformation we could numerically simulate the relation between relief stretch and the skewness statistic. This relation predicts that skewness increases with increasing surface depth. Furthermore, it predicts that the correlation between skewness and illumination can be either positive or negative, depending on the depth range. We experimentally tested whether changes in the depth range and illumination direction alter the appearance. We indeed find a convincingly strong illusory gloss effect on stretched Lambertian surfaces. However, the results could not be fully explained by the skewness hypothesis. We reinterpreted our results in the context of the bas-relief ambiguity (P. N. Belhumeur, D. J. Kriegman, & L. Yuille, 1999) and show that this model qualitatively predicts illusory highlights on locations that differ from actual specular highlight locations with increasing illumination direction.
Analysis oftheParacoccidioidesbrasiliensis triosephosphate isomerase suggests thepotential foradhesin function

Analysis oftheParacoccidioidesbrasiliensis triosephosphate isomerase suggests thepotential foradhesin function Luiz Augusto Pereira, Sônia Nair Báo, Mônica Santiago Barbosa, Juliana Leal M. da Silva, Maria Sueli S. Felipe, Jaime Martins de Santana, Maria José S. Mendes-Giannini & Célia Maria de Almeida Soares Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil; Laboratório de Microscopia, Universidade de Brası́lia, Brası́lia, DF; Laboratório de Micologia Clı́nica, Universidade Estadual Júlio de Mesquita Filho, Araraquara, São Paulo; Laboratório de Biologia Molecular, Universidade de Brası́lia; and Laboratório Interdisciplinar em Doença de Chagas, Universidade de Brası́lia, Brası́lia, DF, Brazil
A Concept for a High Performance Reflector-Based X-Band SAR

The success of current spaceborne Synthetic Aperture Radar (SAR) is boosting the performance requirement of next generation systems. In order to cope with the evolution of SAR the design of the new systems will need to meet higher requirements for spacial and radiometric resolutions together with an increased availability. This tendency is recognized nearly independently of the application area and manifests itself through several study programs initiated by space agencies aiming at the design of future SAR systems. In this context the use of large reflectors combined with digital feed arrays for SAR has not always received adequate attention. This paper suggests an X-band spaceborne SAR system utilizing a deployable reflector together with a digital feed array, analyzes its performance and highlights its advantages compared to other systems based on direct radiating arrays.
THE SLUGGS SURVEY: HST/ACS MOSAIC IMAGING OF THE NGC 3115 GLOBULAR CLUSTER SYSTEM

We present Hubble Space Telescope/Advanced Camera for Surveys (HST/ACS) g and z photometry and half-light radii Rh measurements of 360 globular cluster (GC) candidates around the nearby S0 galaxy NGC 3115. We also include Subaru/Suprime-Cam g, r, and i photometry of 421 additional candidates. The well-established color bimodality of the GC system is obvious in the HST/ACS photometry. We find evidence for a “blue tilt” in the blue GC subpopulation, wherein the GCs in the blue subpopulation get redder as luminosity increases, indicative of a mass–metallicity relationship. We find a color gradient in both the red and blue subpopulations, with each group of clusters becoming bluer at larger distances from NGC 3115. The gradient is of similar strength in both subpopulations, but is monotonic and more significant for the blue clusters. On average, the blue clusters have ∼10% larger Rh than the red clusters. This average difference is less than is typically observed for early-type galaxies but does match that measured in the literature for the Sombrero Galaxy (M104), suggesting that morphology and inclination may affect the measured size difference between the red and blue clusters. However, the scatter on the Rh measurements is large. We also identify 31 clusters more extended than typical GCs, which we term ultra-compact dwarf (UCD) candidates. Many of these objects are actually considerably fainter than typical UCDs. While it is likely that a significant number will be background contaminants, six of these UCD candidates are spectroscopically confirmed as NGC 3115 members. To explore the prevalence of low-mass X-ray binaries in the GC system, we match our ACS and Suprime-Cam detections to corresponding Chandra X-ray sources. We identify 45 X-ray–GC matches: 16 among the blue subpopulation and 29 among the red subpopulation. These X-ray/GC coincidence fractions are larger than is typical for most GC systems, probably due to the increased depth of the X-ray data compared to previous studies of GC systems.
Soft-coupling suspension system for an intradural spinal cord stimulator: Biophysical performance characteristics

We have characterized the mechanical compliance of an improved version of the suspension system used to position the electrode-bearing membrane of an intradural neuromodulator on the dorsal pial surface of the spinal cord. Over the compression span of 5 mm, it exhibited a restoring force of 2.4 μN μm−1 and a mean pressure of 0.5 mm Hg (=66 Pa) on the surface below it, well within the range of normal intrathecal pressures. We have implanted prototype devices employing this suspension and a novel device fixation technique in a chronic ovine model of spinal cord stimulation and found that it maintains stable contact at the electrode-pia interface without lead fracture, as determined by measurement of the inter-contact impedances.
A preliminary attempt to address indomethacins poor water solubility using solid self emulsifying drug delivery system as a carrier

The non-steroidal anti-inflammatory drug (NSAID), indomethacin, is disadvantaged by its poor water solubility and gastrointestinal adverse effects. The aim of this work therefore was to formulate this drug into solid self emulsifying drug delivery system (SSEDDS) as an attempt to improve its aqueous solubility and anti-inflammatory property using shea butter or its blend with Bos indicus lipid. Proximate analysis was carried out on shea butter. Indomethacin-loaded SSEDDS containing lipid (shea butter, Vitellaria paradoxa), surfactant (Tween 65) and cosurfactant (Span 85) or lipid blends (shea butter/Bos indicus fat), surfactant blends (Tween 65/Tween 80) and cosurfactant (Span 85) were formulated. The drug-loaded SSEDDS were evaluated for visual isotropicity, emulsification time, aqueous dilution, refrigeration test, loading efficiency, in vitro drug release and anti-inflammatory properties. Results showed that Shea butter contained 92.8% fat, 1.52% moisture, 2.05% protein, 9.78% carbohydrate and trace amounts of fiber and ash. Pre/post formulation isotropicity tests showed that all batches of indomethacin SSEDDS were stable. SSEDDS containing shea butter as the lipid component recorded significantly (p < 0.05) longer emulsification times than those containing lipid blends. The T85 of most of the batches was within 30 min. Shea butter-based SSEDDS demonstrated significantly (p < 0.05) higher anti-inflammatory effect than unformulated indomethacin powder. In conclusion, Shea butter conferred stability to the formulations and contributed to acceptable in vitro and superior anti-inflammatory characteristics of indomethacin. Key words: Shea butter, Bos indicus fat, indomethacin, solid self emulsifying drug delivery system (SEDDS), anti-inflammatory.
Unexpected understorey community development after 30 years in ancient and post‐agricultural forests

1. Land‐use change is considered one of the most radical and extensive disturbances that have influenced plant distributions and diversity patterns in forest understorey communities in much of Europe and eastern North America. In forests growing on former agricultural land, local species diversity and community differentiation among sites are generally reduced compared with ancient forests (i.e. forests with no historical record of agriculture). Yet, no study has determined how the compositional differences created by former land‐use change over time as the forest sites recover from former agricultural use.
Oscillating Power Minimization in Cascaded H-bridge Converter with Phase-Shifted Carrier PWM

In PWM converters, current ripple is inherently produced by the switching actions. The current ripple would then introduce oscillating power which reduces the efficiency of the system. This paper proposes a new Phase-Shifted Carrier PWM technique for the Multilevel Cascaded H-bridge converter, which employs Carrier Phase Angle Offset to achieve cancellation in the current harmonics. Thus reducing the oscillating power delivered to the load. The mathematical analysis of the proposed modulation technique is presented and shown to provide an significant improvement over the conventional PSC-PWM. Simulation and experimental results are also provided to verified the theoretical analysis.