Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of the human primary visual cortex and cerebellum proteomes using shotgun mass spectrometry‐data‐independent analyses

We present the first characterization of the human occipital lobe (primary visual cortex) and cerebellum proteomes. Proteins were identified using a combination of gel electrophoresis and data‐independent nanoflow liquid chromatography mass spectrometry (nLC‐MSE). The resulting data sets comprised 391 and 330 unique proteins in occipital lobe and cerebellum, respectively, present in at least 75% of the analyzed samples with 297 proteins found in common. These proteins have been associated previously with conditions, such as neurological disorder, progressive motor neuropathy, Parkinson's disease and schizophrenia. The unique proteins identified in the occipital lobe included the interesting finding of growth hormone and several members of the Ca2+‐dependent calmodulin kinase and serine/threonine protein phosphatase families. The complete mapping of these and other brain proteomes may help in the elucidation of neurological processes and identify potential targets for therapeutic strategies.
Usefulness of Scintimammography With Tc-99m MIBI in Clinical Practice

Radiographic mammography (MM) is routinely used to diagnose breast cancer. MM has a number of well-known limitations, especially in cases of a dense or dysplastic breast. Scintimammography (SM) with Tc-99m MIBI has been successfully used as a useful complement to MM. The authors report a case of a 57-year-old woman with MM with tiny calcifications in the left breast, which were classified by the radiologist as probably benign lesions. SM with Tc-99m MIBI showed a focal area of increased uptake in the upper outer quadrant of the left breast. On the basis of SM results, an excisional biopsy was performed and pathologic examination revealed infiltrating ductal carcinoma.
HYDROTHERMAL EXTRACTION OF PHYTOCHEMICAL COMPOUNDS FROM Polygonum cuspidatum ROOTS IN A SEMI-BATCH REACTOR SYSTEM

Hydrothermal extraction is known as a natural and green way for antioxidant compounds extraction. Antioxidant compounds from the roots of traditional Chinese medicinal herb Polygonum cuspidatum (P. cuspidatum) has been extracted at hydrothermal conditions. The antioxidant compounds were identified as polyphenolic compounds of resveratrol, rutin and quercetin. The effect of temperatures on the extraction yield of antioxidant compounds was studied. Based on the result, extraction yields of resveratrol, quercetin, and rutin significantly increased with increasing temperature at 10 MPa. After 180 min of extraction time, the yields of resveratrol, quercetin, and rutin were 0.95, 0.26, and 6.73 mg/g of feed loaded at 473 K, respectively. These results revealed that hydrothermal extraction is applicable method for the isolation of polyphenolic compounds from other types of biomass and may lead to an advanced plant biomass components extraction technology.
Large-scale phosphorylation analysis of mouse liver

Protein phosphorylation is a complex network of signaling and regulatory events that affects virtually every cellular process. Our understanding of the nature of this network as a whole remains limited, largely because of an array of technical challenges in the isolation and high-throughput sequencing of phosphorylated species. In the present work, we demonstrate that a combination of tandem phosphopeptide enrichment methods, high performance MS, and optimized database search/data filtering strategies is a powerful tool for surveying the phosphoproteome. Using our integrated analytical platform, we report the identification of 5,635 nonredundant phosphorylation sites from 2,328 proteins from mouse liver. From this list of sites, we extracted both novel and known motifs for specific Ser/Thr kinases including a “dipolar” motif. We also found that C-terminal phosphorylation was more frequent than at any other location and that the distribution of potential kinases for these sites was unique. Finally, we identified double phosphorylation motifs that may be involved in ordered phosphorylation.
Design of signal acquisition and tracking process based on multi-thread for real-time GNSS software receiver

In this paper, two-thread based signal tracking and acquisition process was designed. In general, the signal acquisition process is performed slower than the tracking process. For the reasons mentioned above, the results of acquisition from a few seconds later can be provide to tracking loop. To solve the problem, we proposed a correction method that the past of code phase can be corrected as currently code phase. We also performed an error analysis of the proposed method whether the corrected phase can apply to signal tracking or not. The performance and accuracy of the correction method is verified through signal tracking and acquisition test using real GNSS signal that was captured by USRP. As a result, although signal acquisition process does not operate on real-time, software receiver can be operated on real-time when the correction method is applied.
Molecular Signature of Quiescent Satellite Cells in Adult Skeletal Muscle

Skeletal muscle satellite cells play key roles in postnatal muscle growth and regeneration. To study molecular regulation of satellite cells, we directly prepared satellite cells from 8‐ to 12‐week‐old C57BL/6 mice and performed genome‐wide gene expression analysis. Compared with activated/cycling satellite cells, 507 genes were highly upregulated in quiescent satellite cells. These included negative regulators of cell cycle and myogenic inhibitors. Gene set enrichment analysis revealed that quiescent satellite cells preferentially express the genes involved in cell‐cell adhesion, regulation of cell growth, formation of extracellular matrix, copper and iron homeostasis, and lipid transportation. Furthermore, reverse transcription‐polymerase chain reaction on differentially expressed genes confirmed that calcitonin receptor (CTR) was exclusively expressed in dormant satellite cells but not in activated satellite cells. In addition, CTR mRNA is hardly detected in nonmyogenic cells. Therefore, we next examined the expression of CTR in vivo. CTR was specifically expressed on quiescent satellite cells, but the expression was not found on activated/proliferating satellite cells during muscle regeneration. CTR‐positive cells reappeared at the rim of regenerating myofibers in later stages of muscle regeneration. Calcitonin stimulation delayed the activation of quiescent satellite cells. Our data provide roles of CTR in quiescent satellite cells and a solid scaffold to further dissect molecular regulation of satellite cells.
Depression, Self-Efficacy, and Oral Health: An Exploration

Aims: To explore the relationship between depression and self-efficacy and adult dental patients� objective and subjective oral health, oral health behaviour, and oral health-related quality of life (OHRQoL). Methods: Survey data and chart review data were collected from 399 regularly scheduled dental patients (173 male/226 female; average age: 50 years; range: 19-93 years). The survey data were collected using a self-administered questionnaire. Depression was measured with the Center of Epidemiological Studies Depression Scale (CESD), generalised self-efficacy with the General Selfefficacy Scale (GSE), and OHRQoL with the Michigan Oral Health-Related Quality of Life Scale�Adult Version (MOHRQoL-A). Results: The more depressed the patients were, the more decayed teeth (r=.13; P=.025) and the fewer restored teeth they had (r=-. 22; P
“How Dark a World It Is … Where Mental Health Is Poorly Treated”: Mental Illness Frames in Sermons Given After the Sandy Hook Shootings

ABSTRACT In the aftermath of the Newtown, CT, massacre, the United States is engaging in public deliberations that will reshape future mental healthcare policies, practices, and systems. We know little about the clergy’s contributions to these deliberations. Clergy, as with psychiatrists and mental health specialists, are members of the helping professions and are regarded as front-line mental health workers and gatekeepers to mental health services. To consider clergy contributions, we drew on Entman’s framing perspective to study sermons given in the state of Connecticut after the Sandy Hook shootings. We examined 73 posted full-text sermons and performed the constant comparative method on 20 that made references to mental illness. We discovered clergy used “social support” and “social system” frames. Upon developing these frames, we discuss the study’s contributions by considering clergy silence, their use of frames to delineate between the secular and the spiritual, their mitigation and promotion of mental illness stigma, and their incomplete social system frame.
Prevalence and clonal diversity of Campylobacter jejuni from dairy farms and urban sources

Abstract AIM: To investigate the role of free-living animals such as spar- rows, rodents and flies as potential reservoirs of Campylobacter spp on a dairy farm, and to assess the genetic diversity among Campylobacter isolates from the farm and an urban source. METHODS: A total of 290 samples (bovine, passerine and ro- dent faeces, and whole flies) were collected from a large com- mercial dairy farm in the Manawatu district in New Zealand, and from faeces from urban sparrows in a nearby city. Other samples collected from the dairy farm included five from silage, two from aprons worn by workers during milking, two from workers' boots and two from water in troughs in a paddock. Isolates of thermophilic Campylobacter spp were identified mor- phologically and phenotypically and further characterised mo- lecularly using pulsed-field gel electrophoresis (PFGE) and the restriction enzyme SmaI. RESULTS: Campylobacter jejuni was the only Campylobacter species isolated from all samples. The highest prevalence was found in faeces from dairy cows (54%), followed by faeces from sparrows from the urban area (40%) and the farm (38%), and from rodents (11%) and whole flies (9%). Other samples from the farm environment such as silage, trough water, and work- ers' aprons and boots were also positive for C. jejuni. Of the 22 restriction patterns obtained, seven were common to more than one source. CONCLUSIONS: Cattle, sparrows, rodents and flies are po- tential reservoirs of C. jejuni on dairy farms. Identical clones of C. jejuni carried by cattle, sparrows, flies and rodents prob- ably indicate a common source of infection. The high level of asymptomatic carriage of C. jejuni by healthy dairy cows could be sufficient to maintain infections within the dairy farm sur- roundings via environmental contamination.
Battery Performance Monitoring and Optimal Observation

Battery Modeling is required to improve the efficiency and reliability of the battery. The Lithium-ion batteries are widely used as a power source for several applications. An accurate battery model and model parameters help in the estimation of the state of charge and state of health. However, battery parameters are variable and depend on several factors such as Temperature, cycle lifetime, the state of charge and depth of discharge and age. By taking account of the characteristics of battery the paper includes circuit oriented model approach of lithium-ion battery. The model characteristics are dependent and linear with respect to the battery’s state of charge. This paper presents a state of charge estimation of lithium ion battery using Kalman filter. Rather than other methods, Kalman filter provides weighted average between the measured value and predicted value. Thus the battery modeling helps to improve the performance of Photovoltaic module and other applications
Cortical and Standard Trajectory Pedicle Screw Fixation Techniques in Stabilizing Multisegment Lumbar Spine with Low Grade Spondylolisthesis

Background Cortical screw (CS) fixation has been recently proposed as an alternative to the standard pedicle screw (PS) fixation technique. Biomechanical studies involving individual screw pullout and single level motion segment stabilization showed comparable performance of both techniques. However, whether this new fixation technique can be applied to the stabilization of multilevel lumbar segments with significant destabilization has been unclear. Purpose To compare stability of CS fixation to the traditional PS fixation in an unstable 3 level spondylolisthesis model. Study Design This is a biomechanical study comparing cortical trajectory pedicle screw fixation to traditional trajectory pedicle screw fixation in an unstable cadaveric model using nondestructive flexibility test. Methods Eight fresh frozen cadaveric lumbar spines (T12- S1) were obtained. After intact baseline testing, a 3-level lowgrade spondylolisthesis was simulated at the L1-4. Each specimen was instrumented with the PS and CS fixation systems. Standard nondestructive flexibility test was performed. Range of motion at each level was compared between the constructs during flexion-extension, lateral bending, and axial rotation. Results The destabilization model significantly increased the ROM in all planes (P<0.05). Both fixation techniques provided significant reduction in the ROM (P<0.05). There was no significant difference in ROM between the PS and CS groups in any of planes (P>0.05). Conclusions Cortical trajectory pedicle screw fixation provided stabilization to multilevel lumbar segment with low-grade spondylolisthesis comparable to the standard trajectory pedicle screw construct.
Are mental illnesses stigmatized for the same reasons? Identifying the stigma-related beliefs underlying common mental illnesses

Abstract Background: Although mental health stigmatization has myriad pernicious consequences, it remains unknown whether mental disorders are stigmatized for the same reasons. Aims: This study identified the stigma-related beliefs that were associated with several common mental illnesses (Study 1), and the extent to which those beliefs predicted stigmatization (Study 2). Methods: In Study 1, we used multidimensional scaling to identify the stigma-related beliefs attributed to nine common mental disorders (e.g. depression, schizophrenia). Study 2 explored whether beliefs commonly associated with depression predicted its stigmatization. Results: In Study 1, we found that the nine mental illnesses differed from each other on two dimensions: social desirability and controllability. In Study 2, we found that regardless of participants’ own depression status, their perceptions that depression is controllable predicted depression-related stigmatization. Conclusions: Our results suggest that stigmatization toward different mental illnesses stem from combinations of different stigmatized beliefs.
Vena Tech-LGM filter: long-term results of a prospective study.

Efficiency and tolerance of the Vena Tech-LGM filter were assessed in 142 patients prospectively studied during a 2-6-year follow-up period. No patients were lost to follow-up, and 137 (91.3%) scheduled control examinations were performed. Fifty-three patients died of causes unrelated to thromboembolic disease. Pulmonary embolism was suspected in five surviving patients (3.5%). Sixteen retractions (18.4%), 16 intracaval migrations (18.4%), and one tilt were observed. Filter patency studied at Doppler ultrasonography, with or without phlebocavography, gave the following results according to the Kaplan-Meier actuarial method: 92% patency after 2 years of follow-up, 80% after 4 years, and 70% after 6 years. Vena Tech-LGM filter obstruction was related to retraction (P < 10(-6)) or distal migration (P < .004). Occurrence of trophic disease in the lower limbs during the follow-up period was related to the initial level of deep venous thrombosis (P = .03) and had no relation to the patency of the filter. The filter was effective in preventing pulmonary embolism, with 70% demonstrating long-term patency, and no deleterious effects were imputed to obstructions observed during follow-up.
An epidemiologic study of oral cancer and precancerous conditions among 101,761 villagers in Maharashtra, India

In an extensive epidemiologic house‐to‐house survey, undertaken to study oral cancer and precancerous conditions, 101,761 villagers were examined in the Poona district in the Maharashtra state in India. Besides detection of 12 oral cancers, prevalences were established for leukoplakia (0.67%), preleukoplakia (0.86%), and submucous fibrosis (0.03%). The sample was also studied for chewing and smoking habits, which were practised by 54.4% of the individuals. Among males the most common habit was chewing tobacco with lime, whereas the dominating habit among females was the use of mishri (burned tobacco). The strongest association found was between bidi smoking and mixed habits and leukoplakia. Only a few cases of leukoplakia were found among mishri users. Except for submucous fibrosis it was found that prevalence does not depend on sex if tobacco habits are taken into account.
The COMPASS Tokamak Plasma Control Software Performance

The COMPASS tokamak has began operation at the IPP Prague in December 2008. A new control system has been built using an ATCA-based real-time system developed at IST Lisbon. The control software is implemented on top of the MARTe real-time framework attaining control cycles as short as 50 μs, with a jitter of less than 1 μs. The controlled parameters, important for the plasma performance, are the plasma current, position of the plasma current center, boundary shape and horizontal and vertical velocities. These are divided in two control cycles: slow at 500 μs and fast at 50 μs. The project has two phases. First, the software implements a digital controller, similar to the analog one used during the COMPASS-D operation in Culham. In the slow cycle, the plasma current and position are measured and controlled with PID and feedforward controllers, respectively, the shaping magnetic field is preprogrammed. The vertical instability and horizontal equilibrium are controlled with the faster 50-μs cycle PID controllers. The second phase will implement a plasma-shape reconstruction algorithm and controller, aiming at optimized plasma performance. The system was designed to be as modular as possible by breaking the functional requirements of the control system into several independent and specialized modules. This splitting enabled tuning the execution of each system part and to use the modules in a variety of applications with different time constraints. This paper presents the design and overall performance of the COMPASS control software.
Ultrasonic calling in 2 species of voles, Microtus pinetorum and M. pennsylvanicus, with different social systems

Abstract When isolated, pups of muroid rodents emit ultrasonic vocalizations, an indication that they are stressed by being alone and exposed to cooling temperatures. Rate of vocalizations is greatest at the end of their 1st week and beginning of the 2nd week, declines in the 2nd week as eyes open and thermoregulation becomes fully established, and ceases in the 3rd week. Young of 1 vole species, the prairie vole (Microtus ochrogaster), vocalize significantly more than those of other species, which has been attributed to the social structure of the species, because they have monogamous mating behavior and high level of biparental care. To determine whether this vocalizing behavior is typical of vole species with monogamous social systems, I examined calling behavior in another monogamous species with biparental care, the pine (or woodland) vole (M. pinetorum), and I compared it to that of a polygynous species that has only maternal care, the meadow vole (M. pennsylvanicus). Pups were isolated for 20 min at 22°C ± 2°C and the number of ultrasonic calls they emitted was monitored. Pine vole pups vocalized at high rates, similar to prairie voles, emitting a mean of 47 calls/min at the ages when calling was greatest (8–16 days). In contrast, meadow vole pups vocalized significantly less, emitting a mean of 9 calls/min at the age of greatest calling (5–9 days). Pine voles continued vocalizing to 23 days, like prairie voles but different from other vole species, including meadow voles, which cease calling earlier. I conclude that young voles of monogamous species, which are highly affiliative and have paternal as well as maternal care, respond to the stress of isolation more strongly than do other vole species and emit more ultrasonic vocalizations than species that are less social and have less parental care.
[Effects of intrauterine contraceptive device on expression of vascular endothelial growth factor, kinase insert domain-containing receptor and microvessel density in endometrium].

OBJECTIVE To investigate the expression of vascular endothelial growth factor (VEGF) and its receptor, kinase insert domain-containing receptor(KDR) and microvessel density (MVD) in endometrium from women wearing fixed copper-intrauterine contraceptive device (IUD, FCu-IUD) or fixed indomethacin-releasing copper-IUD (FICu-IUD). METHODS Twenty healthy women were divided into two study groups: 10 cases wearing the FCu-IUD (FCu-IUD group), 10 cases wearing the FICu-IUD (FICu-IUD group). Immunohistochemical technique was used to determine the expression of VEGF and KDR in endometrium, and the microvessel density (MVD) was counted. The expression of VEGF mRNA was determined by in situ-hybridization. RESULTS Before insertion of FCu-IUD, the expression of VEGF and KDR proteins was 0.357 +/- 0.032 and 0.215 +/- 0.029 respectively. After insertion of FCu-IUD, the expression of VEGF and KDR proteins was 0.568 +/- 0.027 and 0.244 +/- 0.022 respectively, significantly higher than before insertion (P < 0.05). The expression of VEGF mRNA was 0.359 +/- 0.022 before insertion of FCu-IUD, after insertion of FCu-IUD, the expression of VEGF mRNA was 0.425 +/- 0.019 (P < 0.05). There were no significant changes in the level of VEGF protein and mRNA, as well as KDR in endometrium before and after insertion of FICu-IUD. Compared with before insertion of FCu-IUD 15.4 +/- 2.8, a significant increase in MVD was observed after insertion of FCu-IUD 19.8 +/- 4.8, and the expression of VEGF protein was positively correlated with MVD (r = 0.847, P < 0.01). MVD counts were not different significantly before and after insertion of FICu-IUD. CONCLUSIONS FCu-IUD can enhance the expression of VEGF and KDR in the endometrium. FICu-IUD can inhibit the activity of VEGF and KDR by releasing indomethacin. VEGF and KDR may be related to the structural and functional changes of microvessels in endometrium after insertion of FCu-IUD or FICu-IUD.
The land‐potential knowledge system (landpks): mobile apps and collaboration for optimizing climate change investments

Abstract Massive investments in climate change mitigation and adaptation are projected during coming decades. Many of these investments will seek to modify how land is managed. The return on both types of investments can be increased through an understanding of land potential: the potential of the land to support primary production and ecosystem services, and its resilience. A Land‐Potential Knowledge System (LandPKS) is being developed and implemented to provide individual users with point‐based estimates of land potential based on the integration of simple, geo‐tagged user inputs with cloud‐based information and knowledge. This system will rely on mobile phones for knowledge and information exchange, and use cloud computing to integrate, interpret, and access relevant knowledge and information, including local knowledge about land with similar potential. The system will initially provide management options based on long‐term land potential, which depends on climate, topography, and relatively static soil properties, such as soil texture, depth, and mineralogy. Future modules will provide more specific management information based on the status of relatively dynamic soil properties such as organic matter and nutrient content, and of weather. The paper includes a discussion of how this system can be used to help distinguish between meteorological and edaphic drought.