Miracle-dz/UniFAR-FTUs huggingface dataset:
Optimization of a New High Rotary Missile-Borne Stabilization Platform

The passive semi-strapdown roll stabilized platform is an inertial platform, which can isolate the rolling of a projectile body by a special mechanical device. In the passive semi-strapdown roll stabilized platform, the bearing device plays an important role in isolating the rolling of the projectile body. The smaller the friction moment of bearing, the smaller the swing angular velocity of the platform, the smaller the range of inertial sensors required, the higher the accuracy of the navigation solution. In order to further reduce the swing angular velocity of the platform and improve the navigation accuracy, the bearing nested structure that could reduce the friction torque is proposed. Combined with the working principle of the passive semi-strapdown roll stabilized platform, the mechanical calculation model of friction at the moment of bearing the nested structure was established. A series of simulation analysis and tests showed that the output stability value of the friction moment was 47% that of a single bearing; the roll rate of the platform based on the bearing nested structure decreased to 50% of that based on the single bearing structure; the position and attitude errors measured of the platform based on the bearing nested structure decreased to more than 50% of that based on the single bearing structure. It showed that the bearing nested structure could effectively reduce the friction moment, improve the axial reliability of the bearing, and provide a more stable working environment for the passive semi-strapdown roll stabilized platform.
Wintertime Optical Properties of Primary and Secondary Brown Carbon at a Regional Site in the North China Plain.

The light-absorbing properties of atmospheric brown carbon (BrC) are poorly understood due to its complex chemical composition. Here, a black-carbon-tracer method was coupled with source apportionments of organic aerosol (OA) to explore the light-absorbing properties of primary and secondary BrC from the North China Plain (NCP). Primary emissions of BrC contributed more to OA light absorption than secondary processes, and biomass burning OA accounted for 60% of primary BrC absorption at λ = 370 nm, followed by coal combustion OA (35%) and hydrocarbon-like OA (5%). Secondary BrC absorption was high in the early morning, and later decreased due to the bleaching of chromophores. Nighttime aqueous-phase chemistry promoted the formation of secondary light-absorbing compounds and the production of strongly absorbing particles. Source analysis showed that the NCP region was the most important source for primary and secondary BrC sub-types at the study site. The mean direct radiative forcing for BrC was 0.15 W m-2 (0.11 W m-2 and 0.04 W m-2 for the primary and secondary fractions, respectively). This study provides new information on the optical properties of primary and secondary BrC and highlights the importance of atmospheric oxidation on BrC absorption.
Choreography as “Seeing”

Jason Ohlberg, MFA Department of Dance, Skidmore College, Saratoga Springs, NY As dance practitioners in education, our careers have the tendency to embrace hybrid models. As one who professionally identifies with the challenges presented by the teacher/artist/scholar mandate, I’m continually investigating how these three facets of my work might more effectively communicate. I’m specifically interested in how artistic inquiry through interdisciplinary, student-centered processes can engage dancers beyond traditional choreographic practices and offer deeper contexts for learning and growth. This research began through an inter-institutional initiative, funded in-part by the Teagle Foundation, to explore and assess the educational potential of museum exhibition-based pedagogy across disciplines. As part of this initiative, I received two grants from the Frances Young Tang Teaching Museum to work with two separate exhibitions in connection with my performance workshop course in 2017 and 2018. This presented an interesting opportunity to rethink learning outcomes for this course, traditionally designed to facilitate a studio rehearsal process and the creation of choreography for an undergraduate dance concert. The challenge was how to design the experience around a museum-based, student-centered process. This raised several initial questions:
Insights into the IgG heavy chain engineering patent landscape as applied to IgG4 antibody development

ABSTRACT Despite being the least abundant immunoglobulin G in human plasma, IgG4 are used therapeutically when weak effector functions are needed. The increase in engineered IgG4-based antibodies on the market led us to study the patent landscape of IgG4 Fc engineering, i.e., patents claiming modifications in the heavy chain. Thirty-seven relevant patent families were identified, comprising hundreds of IgG4 Fc variants focusing on removal of residual effector functions (since IgG4s bind to FcγRI and weakly to other FcγRs), half-life enhancement and IgG4 stability. Given the number of expired or soon to expire major patents in those 3 areas, companies developing blocking antibodies now have, or will in the near future, access to free tools to design silenced, half-life extended and stable IgG4 antibodies.
Physiological and Transcriptomic Analysis Reveals Distorted Ion Homeostasis and Responses in the Freshwater Plant Spirodela polyrhiza L. under Salt Stress

Duckweeds are a family of freshwater angiosperms with morphology reduced to fronds and propagation by vegetative budding. Unlike other angiosperm plants such as Arabidopsis and rice that have physical barriers between their photosynthetic organs and soils, the photosynthetic organs of duckweeds face directly to their nutrient suppliers (waters), therefore, their responses to salinity may be distinct. In this research, we found that the duckweed Spirodela polyrhiza L. accumulated high content of sodium and reduced potassium and calcium contents in large amounts under salt stress. Fresh weight, Rubisco and AGPase activities, and starch content were significantly decreaseded in the first day but recovered gradually in the following days and accumulated more starch than control from Day 3 to Day 5 when treated with 100 mM and 150 mM NaCl. A total of 2156 differentially expressed genes were identified. Overall, the genes related to ethylene metabolism, major CHO degradation, lipid degradation, N-metabolism, secondary metabolism of flavonoids, and abiotic stress were significantly increased, while those involved in cell cycle and organization, cell wall, mitochondrial electron transport of ATP synthesis, light reaction of photosynthesis, auxin metabolism, and tetrapyrrole synthesis were greatly inhibited. Moreover, salt stress also significantly influenced the expression of transcription factors that are mainly involved in abiotic stress and cell differentiation. However, most of the osmosensing calcium antiporters (OSCA) and the potassium inward channels were downregulated, Na+/H+ antiporters (SOS1 and NHX) and a Na+/Ca2+ exchanger were slightly upregulated, but most of them did not respond significantly to salt stress. These results indicated that the ion homeostasis was strongly disturbed. Finally, the shared and distinct regulatory networks of salt stress responses between duckweeds and other plants were intensively discussed. Taken together, these findings provide novel insights into the underlying mechanisms of salt stress response in duckweeds, and can be served as a useful foundation for salt tolerance improvement of duckweeds for the application in salinity conditions.
DNA analysis of Castanea sativa (sweet chestnut) in Britain and Ireland: Elucidating European origins and genepool diversity

Castanea sativa is classified as non-indigenous in Britain and Ireland. It was long held that it was first introduced into Britain by the Romans, until a recent study found no corroborative evidence of its growing here before c. AD 650. This paper presents new data on the genetic diversity of C. sativa in Britain and Ireland and potential ancestral sources in continental Europe. Microsatellite markers and analytical methods tested in previous European studies were used to genotype over 600 C. sativa trees and coppice stools, sampled from ancient semi-natural woodlands, secondary woodlands and historic cultural sites across Britain and Ireland. A single overall genepool with a diverse admixture of genotypes was found, containing two sub groups differentiating Wales from Ireland, with discrete geographical and typological clusters. C. sativa genotypes in Britain and Ireland were found to relate predominantly to some sites in Portugal, Spain, France, Italy and Romania, but not to Greece, Turkey or eastern parts of Europe. C. sativa has come to Britain and Ireland from these western European areas, which had acted as refugia in the Last Glacial Maximum; we compare its introduction with the colonization/translocation of oak, ash, beech and hazel into Britain and Ireland. Clones of C. sativa were identified in Britain, defining for the first time the antiquity of some ancient trees and coppice stools, evincing both natural regeneration and anthropogenic propagation over many centuries and informing the chronology of the species’ arrival in Britain. This new evidence on the origins and antiquity of British and Irish C. sativa trees enhances their conservation and economic significance, important in the context of increasing threats from environmental change, pests and pathogens.
The role of proximity dimensions and mutual commitment in shaping the performance of university-industry research centres

ABSTRACT Based on a longitudinal study of two university-industry research centres, this paper examines how proximity along the dimensions of social and cognitive proximity and mutual commitment enables partners to comply with the research centres’ goals of developing academic publications and innovations. We propose that social and cognitive proximity are equally important for complying with the goals, and we identify how these proximities co-evolve with actors’ activities and interactions over time. Our main contributions are linked to the relationships among these proximities where repeated contact (social proximity) and mutual commitment are found to be key enablers for developing mutual understanding (cognitive proximity) between firms and university partners.
Early KLRG1+ but Not CD57+CD8+ T Cells in Primary Cytomegalovirus Infection Predict Effector Function and Viral Control

Key Points We report (to our knowledge) novel CMV-specific KLRG1+CD27+ CD57+/− CD8+ TAEFF in primary infection. KLRG1+ TAEFF correlates with CMV function and control in lung transplant patients. CMV remains an important opportunistic pathogen in high-risk lung transplant recipients. We characterized the phenotype and function of CD8+ T cells from acute/primary into chronic CMV infection in 23 (donor+/recipient−; D+R−) lung transplant recipients and found rapid induction of both KLRG1+ and/or CD57+ CMV-specific CD8+ T cells with unexpected coexpression of CD27. These cells demonstrated maturation from an acute effector T cell (TAEFF) to an effector memory T cell (TEM) phenotype with progressive enrichment of KLRG1+CD57+CD27− cells into memory. CMV-specific KLRG1+ TAEFF were capable of in vitro proliferation that diminished upon acquisition of CD57, whereas only KLRG1+ expression correlated with T-bet expression and effector function. In contrast to blood TAEFF, lung mucosal TAEFF demonstrated reduced KLRG1/T-bet expression but similar CD57 levels. Additionally, increased KLRG1+TAEFF were associated with early immune viral control following primary infection. To our knowledge, our findings provide new insights into the roles of KLRG1 and CD57 expression in human T cells, forming the basis for a refined model of CD8+ T cell differentiation during CMV infection.
PERANAN TOTAL QUALITY MANAGEMENT (TQM) DI SEKOLAH DASAR

Management of education by creating a conducive learning environment and successfully fulfilling commitments in fulfilling promises as educational leaders. The low quality of human resources is a fundamental problem that can hamper development and national economic development. Along with the advancement of knowledge and technology, the socio-cultural life, socio-economy is increasingly unacceptable to space and time. In order to build the quality of Indonesian people who are able to challenge it, clearly it requires human resources that can be relied upon in the face of competition. TQM is a quality management system that is related to continuous improvement in improving the quality of education from various aspects of sustainability. Educational quality that is acceptable from customer needs and satisfaction. The application of TQM in school organizations is carried out as an effort to improve the quality of education in schools, thereby improving the quality of schools by improving the quality of education of high quality. The implementation of a management system is always disrupted by balance. Two parties who are pro and contra arise, accept TQM and reject TQM. TQM's refusal because it affects changes in management, namely changing established values. With this pillar, it is expected to be able to help schools improve their educational processes. The implementation of integrated Quality Management in schools is inseparable from the efforts of the principal to provide assistance to the school.
Structure-Based Design and in Silico Screening of Virtual Combinatorial Library of Benzamides Inhibiting 2-trans Enoyl-Acyl Carrier Protein Reductase of Mycobacterium tuberculosis with Favorable Predicted Pharmacokinetic Profiles

Background: During the previous decade a new class of benzamide-based inhibitors of 2-trans enoyl-acyl carrier protein reductase (InhA) of Mycobacterium tuberculosis (Mt) with unusual binding mode have emerged. Here we report in silico design and evaluation of novel benzamide InhA-Mt inhibitors with favorable predicted pharmacokinetic profiles. Methods: By using in situ modifications of the crystal structure of N-benzyl-4-((heteroaryl)methyl) benzamide (BHMB)-InhA complex (PDB entry 4QXM), 3D models of InhA-BHMBx complexes were prepared for a training set of 19 BHMBs with experimentally determined inhibitory potencies (half-maximal inhibitory concentrations IC50exp). In the search for active conformation of the BHMB1-19, linear QSAR model was prepared, which correlated computed gas phase enthalpies of formation (∆∆HMM) of InhA-BHMBx complexes with the IC50exp. Further, taking into account the solvent effect and entropy changes upon ligand, binding resulted in a superior QSAR model correlating computed complexation Gibbs free energies (∆∆Gcom). The successive pharmacophore model (PH4) generated from the active conformations of BHMBs served as a virtual screening tool of novel analogs included in a virtual combinatorial library (VCL) of compounds containing benzamide scaffolds. The VCL filtered by Lipinski’s rule-of-five was screened by the PH4 model to identify new BHMB analogs. Results: Gas phase QSAR model: −log10(IC50exp) = pIC50exp = −0.2465 × ∆∆HMM + 7.95503, R2 = 0.94; superior aqueous phase QSAR model: pIC50exp = −0.2370 × ∆∆Gcom + 7.8783, R2 = 0.97 and PH4 pharmacophore model: pIC50exp = 1.0013 × pIC50exp − 0.0085, R2 = 0.95. The VCL of more than 114 thousand BHMBs was filtered down to 73,565 analogs Lipinski’s rule. The five-point PH4 screening retained 90 new and potent BHMBs with predicted inhibitory potencies IC50pre up to 65 times lower than that of BHMB1 (IC50exp = 20 nM). Predicted pharmacokinetic profile of the new analogs showed enhanced cell membrane permeability and high human oral absorption compared to current anti-tuberculotics. Conclusions: Combined use of QSAR models that considered binding of the BHMBs to InhA, pharmacophore model, and ADME properties helped to recognize bound active conformation of the benzamide inhibitors, permitted in silico screening of VCL of compounds sharing benzamide scaffold and identification of new analogs with predicted high inhibitory potencies and favorable pharmacokinetic profiles.
Optimal Dynamic Appointment Scheduling of Base and Surge Capacity

Problem definition: We study dynamic stochastic appointment scheduling when delaying appointments increases the risk of incurring costly failures, such as readmissions in healthcare or engine failures in preventative maintenance. When near-term base appointment capacity is full, the scheduler faces a trade-off between delaying an appointment at the risk of costly failures versus the additional cost of scheduling the appointment sooner using surge capacity. Academic/practical relevance: Most appointment-scheduling literature in operations focuses on the trade-off between waiting times and utilization. In contrast, we analyze preventative appointment scheduling and its impact on the broader service-supply network when the firm is responsible for service and failure costs. Methodology: We adopt a stochastic dynamic programming (DP) formulation to characterize the optimal scheduling policy and evaluate heuristics. Results: We present sufficient conditions for the optimality of simple policies. When analytical solutions are intractable, we solve the DP numerically and present optimality gaps for several practical policies in a healthcare setting. Managerial implications: Intuitive appointment policies used in practice are robust under moderate capacity utilization, but their optimality gap can quadruple under high load.
Overexpression of miRNA-21 Promotes the Proliferation and Invasion in Hepatocellular Carcinoma Cells via Suppressing SMAD7

Purpose: This study aimed to explore the molecular mechanism of microRNA-21 and smad family member 7 in hepatocellular carcinoma. Method: A total of 57 participants were divided into control group (healthy participants, n = 10) and hepatocellular carcinoma group (hepatocellular carcinoma patients, n = 37). The expression of microRNA-21 levels were first detected in these two groups. Cell transfection was performed on hepatoma cell lines, followed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assay to reveal proliferation and invasion ability. Furthermore, the relation between microRNA-21 and smad family member 7 was revealed by luciferase reporter gene and RNA immunoprecipitation assay. Finally, a transplantation tumor model of breast cancer in mice was constructed. Results: The serum indicators including α-alanine aminotransferase, aspartate aminotransferase, and albumin were differentially expressed between hepatocellular carcinoma group and control group. Compared to the control group, there was a high expression of microRNA-21 in hepatocellular carcinoma group. Low expression of microRNA-21 inhibited the proliferation and invasion of HepG2.2. 15 and Huh7-1.3 cells. Luciferase reporter gene and RNA innumoprecipitation assay showed that smad family member 7 was the target gene of microRNA-21. Moreover, mice model analysis showed that microRNA-21 might regulate the growth of the transplanted tumors in mice by targeting smad family member 7. Conclusion: The upregulated microRNA-21 might participate in the proliferation and migration in cells of hepatocellular carcinoma via suppression of smad family member 7. Furthermore, serum indicators such as alanine aminotransferase, aspartate aminotransferase, and albumin might be used as serum diagnostic markers for hepatocellular carcinoma.
The nsp2 Hypervariable Region of Porcine Reproductive and Respiratory Syndrome Virus Strain JXwn06 Is Associated with Viral Cellular Tropism to Primary Porcine Alveolar Macrophages

The PRRSV nsp2 replicase protein undergoes rapid and broad genetic variations in its middle region in the field, but the underlying significance has remained enigmatic. Here, we demonstrate that the nsp2 hypervariable region not only plays an important regulatory role in maintaining the balance of different viral mRNA species but also regulates PRRSV tropism to primary PAMs. Our results reveal novel functions for PRRSV nsp2 and have important implications for understanding the mechanisms of PRRSV RNA synthesis and cellular tropism. ABSTRACT Porcine reproductive and respiratory syndrome virus (PRRSV) poses a major threat to global pork production and has been notorious for its rapid genetic evolution in the field. The nonstructural protein 2 (nsp2) replicase protein represents the fastest evolving region of PRRSV, but the underlying biological significance has remained poorly understood. By deletion mutagenesis, we discovered that the nsp2 hypervariable region plays an important role in controlling the balance of genomic mRNA and a subset of subgenomic mRNAs. More significantly, we revealed an unexpected link of the nsp2 hypervariable region to viral tropism. Specifically, a mutant of the Chinese highly pathogenic PRRSV strain JXwn06 carrying a deletion spanning nsp2 amino acids 323 to 521 (nsp2Δ323–521) in its hypervariable region was found to lose infectivity in primary porcine alveolar macrophages (PAMs), although it could replicate relatively efficiently in the supporting cell line MARC-145. Consequently, this mutant failed to establish an infection in piglets. Further dissection of the viral life cycle revealed that the mutant had a defect (or defects) lying in the steps between virus penetration and negative-stranded RNA synthesis. Taken together, our results reveal novel functions of nsp2 in the PRRSV life cycle and provide important insights into the mechanisms of PRRSV RNA synthesis and cellular tropism. IMPORTANCE The PRRSV nsp2 replicase protein undergoes rapid and broad genetic variations in its middle region in the field, but the underlying significance has remained enigmatic. Here, we demonstrate that the nsp2 hypervariable region not only plays an important regulatory role in maintaining the balance of different viral mRNA species but also regulates PRRSV tropism to primary PAMs. Our results reveal novel functions for PRRSV nsp2 and have important implications for understanding the mechanisms of PRRSV RNA synthesis and cellular tropism.