Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial Distribution of Surface Soil Organic Carbon Density and Related Factors along an Urbanization Gradient in Beijing

Abstract: Urban surface soil has a unique set of structures and processes that affect surface soil organic carbon density (SOCdensity) and its spatial variations. Using Beijing as a case study, and assisted by field investigations and experiments, we analyzed the spatial distribution of SOCdensity in different land use types and functional regions, and assessed associated factors such as urbanization level, the physiochemical properties of soil and plant configurations. The present study aims to provide useful information about the mechanisms driving soil organic carbon and climate change in developing and developed areas in urbanized regions like Beijing. Results indicate that P is the main factor positively influencing SOCdensity in most regions. Because of the specific interference directly related to human beings in urban areas, with decreases in the urbanization level, more physiochemical factors of soil can influence SOCdensity. SOCdensity under grasses is not significantly different from that under other plant compositions. Urbanization processes decrease the heterogeneity of the spatial pattern of SOCdensity in most land use types, but increased its contents when the area reached a developed level in Beijing. More factors related to human interference and spatial variation of surface soil carbon storage, especially under impervious land in urban areas, should be considered in future studies.
Prospective evaluation of genome sequencing versus standard-of-care as a first molecular diagnostic test

Abstract Purpose: To evaluate the diagnostic yield and clinical utility of clinical genome sequencing (cWGS) as a first genetic test for patients with suspected monogenic disorders. Methods: We conducted a prospective randomized study with pediatric and adult patients recruited from genetics clinics at Massachusetts General Hospital who were undergoing planned genetic testing. Participants were randomized into two groups: standard-of-care genetic testing (SOC) only or SOC and cWGS. Results: 204 participants were enrolled and 99 received cWGS. cWGS returned 23 molecular diagnoses in 20 individuals: A diagnostic yield of 20% (20/99, 95%CI 12.3-28.1%)), which was not significantly different from SOC (17%, 95%CI 9.7%-24.6%, P=0.584). 19/23 cWGS diagnoses provided an explanation for clinical features or were considered worthy of additional workup by referring providers. While cWGS detected all variants reported by SOC, SOC failed to capture 9/23 cWGS diagnoses; primarily due to genes not included in SOC tests. Turnaround time was significantly shorter for SOC compared to cWGS (33.9 days vs 87.2 days, P<0.05). Conclusions: cWGS is technically suitable as a first genetic test and identified clinically relevant variants not captured by SOC. However, further studies addressing other variant types and implementation challenges are needed to support feasibility of its broad-scale adoption.
Adjusted Dynamics of COVID-19 Pandemic due to Herd Immunity in Bangladesh

Amid growing debate between scientists and policymakers on the trade-off between public safety and reviving economy during the COVID-19 pandemic, the government of Bangladesh decided to relax the countrywide lockdown restrictions from the beginning of June 2020. Instead, the Ministry of Public Affairs officials have declared some parts of the capital city and a few other districts as red zones or high-risk areas based on the number of people infected in the late June 2020. Nonetheless, the COVID-19 infection rate had been increasing in almost every other part of the country. Ironically, rather than ensuring rapid tests and isolation of COVID-19 patients, from the beginning of July 2020, the Directorate General of Health Services restrained the maximum number of tests per laboratory. Thus, the health experts have raised the question of whether the government is heading toward achieving herd immunity instead of containing the COVID-19 pandemic. In this article, the dynamics of the pandemic due to SARS-CoV-2 in Bangladesh are analyzed with the SIRD model. We demonstrate that the herd immunity threshold can be reduced to 31% than that of 60% by considering age group cluster analysis resulting in a total of 53.0 million susceptible populations. With the data of Covid-19 cases till July 22, 2020, the time-varying reproduction numbers are used to explain the nature of the pandemic. Based on the estimations of active, severe, and critical cases, we discuss a set of policy recommendations to improve the current pandemic control methods in Bangladesh.
Analytical analysis of piezoelectric field on the optoelectronic characteristics of green GaN-based light-emitting diodes

In this work, we have analyzed the behavior of optoelectronic characteristics i.e. internal quantum efficiency (IQE) by the influence of piezoelectric field. This effect was not considered in widely reported models such as the Standard ABC model, Rate equation model, and phase-space-filling model. In Gallium Nitride (GaN)-based light-emitting diodes (LEDs), the active region is comprised of InGaN/GaN layers. For the emission of green light, high composition of indium is needed in InGaN layer. As a result, the lattice mismatch between GaN and InGaN layer is highly dominated, which leads to strong piezoelectric field. Our proposed calculation by including piezoelectric field shows better agreement with the experimental IQE of green LED. Meanwhile, we have proposed a single quantum well (SQW) device structure with reduced piezoelectric field as compared to the conventional structure.
A new species of Volvariella from India

Volvariella sathei sp. nov., collected from Agharkar Research Institute Campus, Pune, is described, illustrated, and compared with morphologically closely related taxa of Volvariella and Volvopluteus. Diagnostic characters of the new species are the large white fruitbodies, relatively small basidiospores, and cylindric-clavate pleuro- and cheilocystidia. Neighbour-Joining analysis based on ITS sequences confirmed that it is distinct from its closest relative, V. nivea and other morphologically similar Volvariella species. A key to the Indian species of Volvariella and Volvopluteus is provided.
Comparison of potential pollution levels in coastal areas of an Ionian island using hierarchical cluster analysis

The aim of this research is to investigate the potential pollution similarity of the coastal areas using Hierarchical Cluster Analysis method. Furthermore, it is possible to monitor the evolution of tourist facilities in order to identify whether or not potential environmental distortions on the island. So, can be formulated a tourism policy that is in harmony with the island's natural environment. Lefkada is one of the small islands in the Ionian Sea. Geographically the island is situated off the western coast of Greece. Lefkada is often an especially attractive tourist destination with benefits of tourism and ecological costs for two decades. The quality of the environment, both natural and man-made, is essential to tourism especially the coastal areas. Those areas are transitional areas between the land and sea, and they include fragile ecosystems. At the same time, coasts are under very high local and tourism population pressure due to rapid urbanization processes. Massive influxes of tourists, often to a relatively small area, maybe have a huge impact on the environment. Eleven surveyed coastal areas were processed and grouped using the Hierarchical Cluster Analysis. This method is an exploratory technique that evaluates the similarities of potential pollution levels between coastal areas of Lefkada.
Ordered models for concept representation

Basic notions linked with concept theory can be accounted for by partial order relations. These orders translate the fact that, for an agent, an object may be seen as a better or a more typical exemplar of a concept than anyother. They adequately model notions linked with categorial membership, typicality and resemblance, without any of the drawbacks that are classically encountered in conjunction theory. An interesting consequence of such a concept representation is the possibility of using the tools of non-monotonic logic to address some well-known problems of cognitive psychology. Thus, conceptual entailment and concept induction can be reexamined in the framework of preferential inference relations. This leads to a rigorous definition of the basic notions used in the study of category-based induction.
Abstract 10: Cardiac-specific Yap Activation Improve Cardiac Function And Survival In An Experimental Murine Mi Model

Background: Yes-Associated Protein (YAP), the terminal effector of the Hippo signaling pathway, is crucial for regulating embryonic cardiomyocyte proliferation. We hypothesized that YAP activation after myocardial infarction would preserve cardiac function and improve survival. Methods and Results: In this study, we used a cardiac-specific, inducible expression system to activate YAP in adult mouse heart. Activation of YAP in adult heart promoted cardiomyocyte proliferation and did not deleteriously affect heart function. Furthermore, YAP activation after myocardial infarction (MI) preserved heart function and reduced infarct size. Using adeno-associated virus subtype 9 (AAV9) as a delivery vector, we expressed human YAP in the adult murine myocardium immediately after MI. We found that AAV9:hYAP significantly improved cardiac function and mouse survival. AAV9:hYAP did not exert its salutary effects by reducing cardiomyocyte apoptosis. Rather, AAV9:hYAP stimulated adult cardiomyocyte proliferation. Gene e.
Techno stress among medical freshmen: An exploratory study

INTRODUCTION: Use of technology and ICT tools is ubiquitous among college students including medical students. However overuse or misuse may lead to many physical and psychological problems including the risk of harming professional standing in the long term. The purpose of this study was to explore techno stress among freshmen at a medical institute and to suggest measures to reduce problematic use of technology pertaining to internet. OBJECTIVES: (i) To explore the extent of technology use including internet among medical freshmen, (ii) To identify the content of usage (iii) To quantify the extent of techno stress and iv. To suggest measures to reduce problematic use of technology. MATERIALS AND METHODS: The sample comprised all the freshmen at a medical institute (n = 61). A semi structured questionnaire was used to collect socio demographic details of students and their patterns of technology usage. Perceived Stress Scale was used to measure stress. RESULTS: The mean age of the sample was 18.34 (±0.58) years comprising 68% males and 32% females. Students spent a mean time of 3.96 h/day on the technology tools (standard deviation = 4.99). Ninety-two percent of the students used technology for educational purposes followed by entertainment (89%), social networking (77.78%), watching films (70.37%), communication (66.67%) and watching pornography (46.3%). 11.47% had symptoms suggestive of problematic internet use. One percent had craving suggestive of addiction. All these had stress out of which 43% had high and 57% had moderate levels of stress. DISCUSSION: Considering the students' present usage of social media, it can serve as a potent academic tool. However, awareness is required to be raised with regard to its use as a medical professional. CONCLUSION: Use of technology is crucial in the current academic world and restrictions on usage may neither be practical nor feasible. However, understanding patterns of usage among students is a preliminary step that can enable educators and administrators to formulate guidelines to channelise their usage in more productive ways.
Investigation of the relationship of perceived social support and spiritual well-being with postpartum depression

BACKGROUND: Postpartum depression is a significant common health problem that has negative effects on mental and physical health of mothers and their infants. The main purpose of this study was to investigate the relationship between spiritual well-being (SWB) and perceived social support with postpartum depression in new mothers. MATERIALS AND METHODS: Using a descriptive survey design, 200 mothers in the 4th–8th weeks after delivery, who referred to selected therapeutic centers in Qom Province, were selected by a convenience sampling method. They were asked to answer the question of the Edinburgh Postnatal Depression Scale, Multidimensional Scale of Perceived Social Support, SWB Scale, and Farhangestan Spiritual Health Questionnaire. Data were analyzed using Pearson correlation and Chi-square. RESULTS: The prevalence of postpartum depression in this group was 22%. Pearson correlation test showed that there was a negative correlation between high level of perceived social support and SWB with postpartum depression. CONCLUSION: The findings of this study suggest that perceived social support and SWB have an important role in low depressive symptom in mothers during postpartum. This result can help health-care professionals to pay much attention to social support and SWB as a protective factor against postpartum depression in postpartum or pregnancy care programs.
Parental influences on medical students’ self-efficacy and career exploration in collectivist culture

INTRODUCTION: Families, especially parents, play an important role in identifying their children's talents and directing their educational journey. The role of parents in their children career applies both to collectivist and to noncollectivist culture. AIM: To examine the correlation between parental influences on medical students' self-efficacy and career exploration in collectivist culture. METHODS: This research was a quantitative study. The study involved 1017 medical students of medical faculties in four faculties of medicine in Indonesia. All data were collected online in August 2018. The study was conducted using an online survey questionnaire and analyzed to finally form a model that displays the determinants of career exploration behavior. The data were analyzed using the maximum likelihood estimation in IBM AMOS 24. RESULTS: The results found a model that has various routes toward career exploration in collectivist culture. Path analysis revealed both direct and direct effect toward the variable studied. Parents' expectations had influence on self-efficacy. CONCLUSION: The findings show the important role between the influence of parents expectations for self-efficacy and career exploration in children.
The challenges of supporting nursing students in clinical education

Clinical education requires creating a supportive atmosphere for learners and nursing students, who need support to be prepared for their future profession. The purpose of this study is to investigate the challenges of supporting the nursing students in clinical education. This article reviewed studies conducted between 2009 and 2019 with the keywords including support, challenge, students, education, and nursing using SID, Magiran, IranMedex, Google Scholar, Elsevier, and PubMed as databases. Among the 926 articles obtained during the two review stages, which were based on the inclusion and exclusion criteria and the first one analyzed the title and the abstract and the second one analyzed the whole article, ultimately 32 articles were selected for the study. The types of articles used in this study are as follows: qualitative (16 articles), descriptive analytical and cross-sectional (12 articles), review (3 articles), and mixed-methods (1 article). The focus of all studies was on the students' clinical education environment. The participants of the studies were undergraduate nursing students (in 22 articles), graduate students (in 2 articles), nursing instructors and students (in 4 articles), and nursing instructors and managers (in 1 article). Most of the challenges of supporting the students included violence, fear, and anxiety; socio-cultural challenges, environmental-organizational stresses; and ineffective clinical education. The findings of this study showed that nursing students face many challenges in the clinical setting. Therefore, the managers of educational and health-care centers should give a high priority to their students' support programs.
The association of sialyl Lewisa antigen with the metastatic potential of human colon cancer cells

Background: The vascular endothelium plays a critical role in cancer metastasis by directing circulating cancer cells into extravascular tissues and by expressing cell adhesion molecules. The authors investigated the interaction between a cell line derived from human colon cancer and cultured murine endothelial cells, in vitro, and in vivo. Materials and Methods: Variant cell lines with high (WiDr-HA) or low (WiDr-LA) levels of cell surface sialyl Lewis a antigen (s-Le a ) were isolated from the heterogeneous WiDr cells (WiDr-P) in order to characterize the biological behavior of colon cancer cells having elevated cell surface contents of s-Le a . Results: A correlation was found to exist between the degree of s-Le a expression, and the attachment of cancer cells to activated human umbilical vein endothelial cells, or to F-2 cells isolated from murine vascular endothelial cells. The adhesion of WiDr-P and WiDr-HA to F-2 cells was inhibited by the addition of anti-s-Le a antibody (Ab). WiDr-P and WiDr-HA both demonstrated the capacity to metastasize to the liver, by the inoculation of cancer cells to the spleen, while WiDr-LA did not do so. The number of metastatic nodules of WiDr-HA was significantly higher than that of WiDr-P. Treatment with anti-s-Le a Ab inhibited the metastasis of WiDr-P and WiDr-HA to the liver, but not with anti-s-Le x Ab. Conclusions: These findings suggest that s-Le a plays an important role in cell adhesion molecules, in the metastasis of colon cancer.
Theoretical Modelling of Potential Chk1 Inhibitors

In this contribution, we attempted to design novel inhibitors of the serine/threonine-protein kinase Chk1. After studying the interaction of Chk1 ATP binding site with known inhibitor C39, we created seven modified C39-based mole- cules in order to achieve higher binding potentials. Of those, modified molecules 2, 4, 6 and 7 (MD2, MD4, MD6 and MD7) were selected to be assembled in three new molecules, originating MD8, MD9 and MD10. When compared to C39, MD8 and MD9 showed significant improvements in the binding energy while MD10 had a smaller gain. MD9 achieved the best improvement (21%) and MD8 the second best (19%) while MD10 only reached a 6% improvement.
Loss of Anks6 leads to YAP deficiency and liver abnormalities.

ANKS6 is a ciliary protein that localizes to the proximal compartment of the primary cilium, where it regulates signaling. Mutations in the ANKS6 gene cause multiorgan ciliopathies in humans, which include laterality defects of the visceral organs, renal cysts as part of nephronophthisis and congenital hepatic fibrosis (CHF) in the liver. Although CHF together with liver ductal plate malformations (DPM) are common features of several human ciliopathy syndromes, including nephronophthisis-related ciliopathies, the mechanism by which mutations in ciliary genes lead to bile duct developmental abnormalities is not understood. Here, we generated a knockout mouse model of Anks6, and show that ANKS6 function is required for bile duct morphogenesis and cholangiocyte differentiation. Loss of Anks6 causes ciliary abnormalities, ductal plate remodeling defects and periportal fibrosis in the liver. Our expression studies and biochemical analyses show that biliary abnormalities in Anks6-deficient livers result from the dysregulation of YAP transcriptional activity in the bile duct-lining epithelial cells. Mechanistically, our studies suggest, that ANKS6 antagonizes Hippo signaling in the liver during bile duct development by binding to Hippo pathway effector proteins YAP1, TAZ and TEAD4 and promoting their transcriptional activity. Together, this study reveals a novel function for ANKS6 in regulating Hippo signaling during organogenesis and provides mechanistic insights to the regulatory network controlling bile duct differentiation and morphogenesis during liver development.
Overview of gene therapy in spinal muscular atrophy and Duchenne muscular dystrophy

Both 5q‐linked spinal muscular atrophy (SMA) and Duchenne muscular dystrophy (DMD) are fatal monogenic neuromuscular disorders caused by loss‐of‐function mutations. SMA is an autosomal recessive disorder affecting motor neurons that is typically caused by homozygous whole‐gene deletions of SMN1. DMD is an X‐linked recessive muscle disease most often due to exon deletions, but also duplications and smaller sized variants within the DMD gene. Gene replacement therapy offers the opportunity to correct the underlying genetic defect by the introduction of a functional gene. We review the transformative work from clinical trials to United States Food and Drug Administration approval of onasemnogene abeparvovec‐xioi in SMA and its application in clinical practice and the early results of microdystrophin delivery in DMD. We also review the introduction of antisense oligonucleotides to alter pre‐messenger RNA splicing to promote exon inclusion (as in nusinersen in SMA) or exclusion (as in eteplirsen in DMD) into neuromuscular therapeutics. There are multiple promising novel genetically mediated therapies on the horizon, which in aggregate point towards a hopeful future for individuals with SMA and DMD.
Calmodulin Binding to Connexin 35: Specializations to Function as an Electrical Synapse

Calmodulin binding is a nearly universal property of gap junction proteins, imparting a calcium-dependent uncoupling behavior that can serve in an emergency to decouple a stressed cell from its neighbors. However, gap junctions that function as electrical synapses within networks of neurons routinely encounter large fluctuations in local cytoplasmic calcium concentration; frequent uncoupling would be impractical and counterproductive. We have studied the properties and functional consequences of calmodulin binding to the electrical synapse protein Connexin 35 (Cx35 or gjd2b), homologous to mammalian Connexin 36 (Cx36 or gjd2). We find that specializations in Cx35 calmodulin binding sites make it relatively impervious to moderately high levels of cytoplasmic calcium. Calmodulin binding to a site in the C-terminus causes uncoupling when calcium reaches low micromolar concentrations, a behavior prevented by mutations that eliminate calmodulin binding. However, milder stimuli promote calcium/calmodulin-dependent protein kinase II activity that potentiates coupling without interference from calmodulin binding. A second calmodulin binding site in the end of the Cx35 cytoplasmic loop, homologous to a calmodulin binding site present in many connexins, binds calmodulin with very low affinity and stoichiometry. Together, the calmodulin binding sites cause Cx35 to uncouple only at extreme levels of intracellular calcium.