Miracle-dz/UniFAR-FTUs huggingface dataset:
Antiproliferative, apoptotic induction, and antiinvasive effects of Leersia hexandra (L.) Sw., Panicum repens Linn., and Brachiaria mutica (Forsk.) Stapf extracts on human cancer cells.

In vitro study of antiproliferation and cytotoxicity of the hexane and butanolic extracts of the three grass species, namely Leersia hexandra (L.) Sw. (“Yaa sai”), Panicum repens Linn. (“Yaa channakaat”), and Brachiaria mutica (Forsk.) Stapf (“Yaa khon”) demonstrated selective antiproliferative properties in both human lung cancer A549 and cervical cancer HeLa cell lines relative to normal human lung fibroblasts MRC-5, with significant differences in IC 50 values (p<0.05). The butanolic extract of P. repens Linn. displayed the strongest growth inhibition in A549 and HeLa cells (IC 50 2 and 1.1 mg/ml, respectively), whereas all other extracts exhibited only moderate to low antiproliferative effects (IC 50 values, 90-980 mg/ml). Most grass extracts induced cell lethality at higher concentrations, suggesting their cytotoxic effects. The ELISA-based apoptosis assay showed that the hexane extract of these grasses triggered a significant increase in the level of apoptosis (p<0.05) in treated A549 cells. However, all the extracts induced ladder-like DNA fragmentation in both tumor cell lines in a doseand time-dependent manner, suggesting that these extracts exhibit cytotoxicity through apoptotic induction. The study of anti-invasive effects of the three grass species revealed that the hexane extract of L. hexandra (L.) Sw. (50-100 g/ml) and butanolic extract of P. repens Linn. (1 g/ml) effectively reduced the invasive capacity of MDA-MB-231 cells.
The first complete chloroplast genome of a mangrove fern, Acrostichum speciosum

Abstract Acrostichum genus is the only mangrove fern that distribute in the intertidal zones of tropical and subtropical regions. A. speciosum is one rare species occurring in only few locations in Hainan and Guangdong, China. To study the evolutionary history of fern will improve our understanding of the origin of vascular plants. Here, we provide the complete chloroplast genome of A. speciosum, which is 156,095 bp in length with a GC content of 38.45%. A pair of identical inverted repeat regions (IRs) of 24,938 bp separate a large single copy (LSC) region of 84,476 and a small single copy (SSC) region of 21,744 bp. A total of 112 genes were annotated, including 72 protein-coding genes, 32 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 30 ferns showed that A. speciosum and Ceratopteris richardii, both are aquatic plants, formed an original base clade in Pteridaceae. Our work reports the complete chloroplast genome of mangrove fern A. speciosum, which will help to improve understanding of the evolutionary history of ferns.
Manipulating the biological character of red algae extract ( kappaphycus alvarezii ) administration on the total cholesterol levels of wistar strain male rats ( rattus norvegicus ) induced by a high-fat diet

Background/aim: Cholesterol is recycled in the body. The liver excretes cholesterol into biliary fluids, which is then stored in the gallbladder, which then excretes it in a non-esterified form (via bile) into the digestive tract. Typically, about 50% of the excreted cholesterol is reabsorbed by the small intestine back into the bloodstream Cholesterol is one of several biological problems that can result in death, especially for high-risk groups, such as older people and those with hypertension and hypercholesterolemia, and can lead to atherosclerosis and reduce work productivity as well. Red algae extract (Kappaphycus alvarezii) affects the total cholesterol level because the content of β-carotene reduces the formation and oxidation from low-density lipoprotein proteins (LDL cholesterol). This study aimed to determine the effect of red algae extract (Kappa hycusalvarezii) administration with a dose of 140 mg/200grBW on the total cholesterol levels of Wistar strain male rats (Rattus norvegicus) induced by a high-fat diet. Materials and methods: This research used 30 rats that met inclusion requirements, and they were divided randomly into three groups: the normal group (K0), feed group (K1), and Kappaphycus alvarezii group (K2). An examination of cholesterol levels was done for each group on the 31st day. The sample was analyzed using the CHOD-PAP (Cholesterol oxidase para aminophenazone) method. The data were analyzed using a one-way ANOVA test. Results: Statistics based on Mann-Whitney U tests for significance levels between the group that had not been given and the other group that had been given the extract of red algae (Kappaphycus alvarezii) with a dose of 140 mg/200grBW for rats that had been given a high-fat diet were less than 0.05 (Sig<0.05), resulting in 0.001. Conclusions: Administering the extract of red algae (Kappaphycus alvarezii) with a dose of 140 mg/200grBW affected the total cholesterol level of Wistar strain male rats (Rattus norvegicus) inducted with a high-fat diet.
Detection of Aflatoxin in Aspergillus Species Isolated from Pistachio in Iran

To estimate the incidence contamination of fresh pistachio nuts by aflatoxigenic fungi in Iran, nut samples were collected from pistachio orchards in Kerman, Rafsanjan and Isfahan regions. Out of the 200 Aspergillus isolates obtained, 11 species were identified as A. alliaceous, A. candidus, A. flavus, A. niger, A. niveus, A. ochraceus, A. parasiticus, A. tamari, A. terreus, A. unguis and A. wentii. For detection of aflatoxin production ability of the isolates, three target genes, namely aflR, aflJ, and omtB, used in PCR amplification. In all the examined cases, the degenerate primer designed for amplification of omtB gene, named omtBII, was able to amplify an expected 611 bp fragment in aflatoxigenic isolates in this study and yielded the same result as those obtained from TLC analysis and fluorescence ability by application of methylated b-cyclodextrin in culture media. Using this procedure the significant incidence of aflatoxin-producing aspergilli was confirmed in pistachio nuts produced in different regions of Iran. The results indicated that PCR method described here, in combination with fluorescence assay, is a reliable and simple confirmatory test for monitoring pistachio nuts contaminated with aflatoxinogenic aspergilli.
MORPHOLOGY OF THE INNER SIDE OF THE MANDIBLE IN MICROMAMMALS (MAMMALIA: INSECTIVORA, CHIROPTERA, RODENTIA) OF ROMANIA

In this paper, the inner side of the mandible of 39 Romanian micromammal species (Mammalia: Insectivora, Chiroptera, Rodentia) is described and illustrated. The author proposed the name of some structures observed on the inner side of the mandible as: fossa ramus mandibulae (FRM), processus angulus mandibulae (PAM), incisura mandibulae superior (IMS), incisura mandibulae inferior (IMI), crista ramus mandibulae (CRM), processus corpus mandibulae (PCM). Resume. Dans ce travail la face interne de la mandibule est illustree et decrite chez 39 especes de micromammiferes (Mammalia: Insectivora, Chiroptera, Rodentia) de Roumanie. L'auteur propose de denommer certaines structures observees sur la face interne de la mandibule: fossa ramus mandibulae (FRM), processus angulus mandibulae (PAM), incisura mandibulae superior (IMS), incisura mandibulae inferior (IMI), crista ramus mandibulae (CRM), processus corpus mandibulae(PCM).
The Essential Amino Acid Requirements of Infants

textabstractInfancy is a period characterized by a high growth velocity and a high rate of physiological development. Nutrition plays a major role in determining health during this critical period of life. Moreover, early nutrition has a programming effect on health in later life. Early nutrition has been found to be associated with obesity, coronary heart disease, neurodevelopmental outcome, cardiovascular and allergic diseases in later life. These associations are supported by evidence from experimental studies in infants.
Neutral to Lombard Speech Conversion with Deep Learning

In this paper, we propose several approaches for neutral to Lombard speech conversion. We study in particular the influence of different recurrent neural network architectures where their main hyper-parameters are carefully selected using a bandit-based approach. We also apply the Continuous Wavelet Transform (CWT) as a multi-resolution analysis framework to better model temporal dependencies of the different features selected. The speech conversion results obtained are validated by means of objective evaluations which highlight in particular the interest of the wavelet transform for the learning process.
Arabic sign language intelligent translator

ABSTRACT Arabic sign language (ArSL) is method of communication between deaf communities in Arab countries; therefore, the development of systemsthat can recognize the gestures provides a means for the Deaf to easily integrate into society. In this research we implemented a computational structurefor an intelligent interpreter that automatically recognizes the isolated dynamic gestures. The proposed system recognizes and translates gesturesperformed with one or both hands. It comprises five subsystems, building dataset, video processing, feature extraction, mapping between ArSL and Arabictext, and text generation. To apply the system, 100-signs of ArSL was used, which was applied on 1500 video files. It's were divided into five classes:alphabet, numbers, "prepositions, pronouns and question words", Arabic life expressions, and "nouns and verbs". The evaluation indicated that thesystem automatically recognizes and translates isolated dynamic ArSL gestures by highly accurate manner. The results showed that the system accuracy is 95.8%.
Parental allocation in Acipenser naccarii– statistical aspects and software development

Summary The parental allocation (PA) in aquaculture allows the association between progeny and broodstock stocks even if the different families of offspring are reared in the same tank. Fingerprinting is needed for PA, and microsatellites are the codominant and polymorphic markers mainly used for this purpose. The simplest method of PA is the exclusion approach, based on the Mendelian mode of inheritance. The rejection of a parent-offspring hypothesis is based on incompatibility between DNA-profiling of offspring and parents. When the exclusion method is not possible a likelihood approach can be used, therefore the progeny is assigned to parents which are not excluded using the likelihood scores derived from their genotypes. Parentage is ambiguous when more parents present the highest non zero likelihood. The software for PA is disposable for diploids, but not for polyploids like sturgeons, in particular Acipenser naccarii which is a functional tetraploid species. To solve the problems concerning to the PA in tetraploids a Windows-based software was developed, using the exclusion method and likelihood approach in the case of multiple allocations. A simulation computer-procedure was also developed to validate the software algorithms. The total results obtained were: correct allocations 93.7%; ambiguous allocations 2.1% and erroneous allocations 4.2%.
How Significant Was the 1877/78 El Niño?

Previous research has shown that the 1877/78 El Niño resulted in great famine events around the world. However, the strength and statistical significance of this El Niño event have not been fully addressed, largely due to the lack of data. We take a closer look at the data using an ensemble analysis of the Extended Reconstructed Sea Surface Temperature version 5 (ERSSTv5). The ERSSTv5 standard run indicates a strong El Niño event with a peak monthly value of the Niño-3 index of 3.5°C during 1877/78, stronger than those during 1982/83, 1997/98, and 2015/16. However, an analysis of the ERSSTv5 ensemble runs indicates that the strength and significance (uncertainty estimates) depend on the construction of the ensembles. A 1000-member ensemble analysis shows that the ensemble mean Niño-3 index has a much weaker peak of 1.8°C, and its uncertainty is much larger during 1877/78 (2.8°C) than during 1982/83 (0.3°C), 1997/98 (0.2°C), and 2015/16 (0.1°C). Further, the large uncertainty during 1877/78 is associated with selections of a short (1 month) period of raw-data filter and a large (20%) acceptance criterion of empirical orthogonal teleconnection modes in the ERSSTv5 reconstruction. By adjusting these two parameters, the uncertainty during 1877/78 decreases to 0.5°C, while the peak monthly value of the Niño-3 index in the ensemble mean increases to 2.8°C, suggesting a strong and statistically significant 1877/78 El Niño event. The adjustment of those two parameters is validated by masking the modern observations of 1981–2017 to 1861–97. Based on the estimated uncertainties, the differences among the strength of these four major El Niño events are not statistically significant.
Motivations for Sexual Identity Concealment and Their Associations with Mental Health among Bisexual, Pansexual, Queer, and Fluid (Bi+) Individuals

Abstract Bisexual and other non-monosexual (bi+) people are at increased risk for depression and anxiety compared to both heterosexual and gay/lesbian people. Bi + people are also more likely to conceal their sexual orientation than gay/lesbian people are, and concealment is generally associated with negative mental health outcomes. Despite evidence that concealment is a particularly salient stressor for bi+ people, there has been a lack of attention to their motivations for concealment. As such, the goal of the current study was to examine the associations among concealment, motivations for concealment, and depression and generalized anxiety symptoms in a sample of 715 bi+ people who completed an online survey. Nearly half of participants endorsed purposely trying to conceal their bi+ identity in their day-to-day life, and concealment was significantly associated with higher levels of depression and generalized anxiety. Using exploratory factor analysis, we identified two motivations for concealment: intrapersonal motivations (e.g., one’s bi+ identity not being a central part of one’s overall identity, not being comfortable with being bi+) and interpersonal motivations (e.g., concern about being judged or treated negatively, concern about putting oneself at risk of physical harm). Interpersonal motivations were significantly associated with higher levels of depression and generalized anxiety, whereas intrapersonal motivations were not. In sum, while concealment may generally be associated with negative mental health outcomes, this may only be the case for those who conceal out of concern for discrimination and victimization. These findings highlight the importance of examining bi+ people’s motivations for concealing their sexual orientation in order to understand the extent to which they experience negative mental health outcomes.
A Novel Product Recommendation Approach with Modified Self-Attention Mechanism and Neural Network

: Product recommendation is a technique that is being applied to most of the ecommerce platforms and for different businesses to analyze buyer’s behavior periodically. Unsupervised Learning finds a natural fit with unlabeled data. This leads to efficient principal component analysis and in such way we can understand the data and its properties. Moving beyond the age-old techniques, if we use latest machine learning with deep learning algorithms, we are able to calculate right products and the high-dimensional space based recommendation techniques. This enables us to use different clustering and classification methods, such as k-means and k-medians and hierarchical clustering and SVM, NB and random forest classification. We also use Shannon entropy and mean squared error to find optima. If the input data are labelled, then an exponential speedup is feasible over classical algorithms with the proposed algorithm, which is capable of their self-analysis for better results.
HEPATIC MICROSPORIDIOSIS OF JUVENILE GREY MULLET, CHELON LABROSUS, WITH PARTICULAR REFERENCE TO PARASITE DEVELOPMENT AND TRANSMISSION

·-· . -. INTROOOCTION . ·-· . -. -. -_ . -. 2 REVIEW . ·-· . 5 Historical records of fish microsporidiosis; taxonomy of the group •••••. •. • ,_, ••••. • •:• ••••. ,_, •••. ••. •••••••• 5 Transmission of microsporidiosis, in fish·hosts and in vitro . , . 12 Ultrastructure, development and host/parasite interactions . ·-· . 1 7 MATERIALS AND METHOOO . ·-. 24 Chelon labrosus • • • ,. , • • • • • • • • • • ,-, ,. , • • • • • e:• • • • • • • • • • • ,. , 24 Collection •• •:• ••••• ·-· ••••• ·=· •••••••••••••. •••••• 24 Maintenance . 24 Husbandry . 25 Anaesthetics . ·-· . -. 25 Introduction of spore suspensions into fish 25 Aseptic removal of liver 26 Microaemma hepaticus identification and removal of parasite stages ••. •. ••. ••••. •. ••. ••. ••••••••••• 26 Screening for infection . 26 Extraction of spores . -. 26 Spore viability . 27 Spore suspension concentration . ~ . 27 Preparation of reagents used in investigations of filament extrusion . •:• . •:• . 28 Biological extracts . ·-. 28 Enzyme preparations . 28 Oxidising agents . 28 Pretreatment media . -. 29 Sterilisation of instruments, glassware and solutions 29 RTG cell culture . ,,, ,, . Source of cells Growth medium Subculture ·-· . ,, . •-• . ·-· . ·-· ·-· . Susp·ension cultures • •-• . _ ••. , •. • ,_, ••••. _ . •. •. ••••• Coverslip cultures •• , ••. •••. •••••• , , •••. •. •. Examination of cultures . -. Primary culture of c. labrosus liver cells . •-• . Source of material Culture·media . Tissue dissociation and culture procedure Histology . Fresh material Wax histology . Electron microscopy . ·-· . ·-· . ·-· Photography . •-• . ·-· . Live fishes . -. -. Macrophotography Photomicrography EXPERIMENTS AND RESULTS . -. Chelon . labrosus • • • e:• e:e • • • • • • • • • • • • •·• e:e • • • • • •·• • • • 1.1 • • • Seasonal occurrence and size range Anatomy and histology of the liver •• ' •• . I •••• ,I ••• I I I I I I I I I. I I I I DE'scription of Microgemma hepaticus gen.n. sp.n. Merogony ••• , •• , ••••••••••• _, •••••••••••••••••• _, • SJ? C? ro_gony ••••••••••••••••••••••••. •••••••••••••• Nuclear division Sporogenesis Spore structure Page 29 29 29
Coronavirus Spreads and Bitcoin's 2020 Rally: Is There a Link ?

The coronavirus epidemic is not the first virus outbreak that has threatened to disturb financial markets. But the world is now more interconnected since the 2003 SARS outbreak as global companies' revenues have become much more exposed to China. The purposes of this paper are threefold. The first is to address the timely question of whether Bitcoin exhibits a safe-haven property against heightened uncertainty over how the duration and spread of the coronavirus could hit the world economy. The second purpose is to assess if the initial news of the coronavirus outbreak have led to an increased volatility of Bitcoin. The third aim is to test if Bitcoin immediately react on publicly announced information (follows the hypothesis of efficient markets). We show that the current bullish sentiment is triggered by investors seeking Bitcoin as a safe haven in the uncertain times ahead. But we also find that the virus intensifies the volatility of Bitcoin due to a search by investors for alternative asset classes amid concerns about the coronavirus. The information regarding the coronavirus takes time to be reflected in the Bitcoin price, highlighting the associated inefficiencies it brings. Also, the risk to global markets may currently be masked owing to wide liquidity injections by Central Banks including the People's Bank of China and the U.S Federal Reserve.
Nuevos datos de distribución de especies autóctonas de peces de agua dulce de España

Over the last two years (2008-2010) we performed a monitoring project to update the Spanish freshwater fish fauna. We have collected in 785 sampling localities homogeneously distributed throughout Spain each year. We find as the most important result of this study the occurrence of several species that constitute new records for some rivers or basins in Spain: Lampetra planeri, Petromyzon marinus, Barbus haasi, Iberochondrostoma lemmingii, Squalius alburnoides, Squalius carolitertii, Squalius malacitanus, Cobitis calderoni, Cobitis paludica , and Salaria fluviatilis . Finally we corroborate the existence of Barbatula barbatula in the Duero basin and the assignation of the northeastern populations of this genus to B. quignardi .
Myelin Membrane Growth and Organization in a Cellular Model System (EN)

This thesis addresses the two fundamental questions of myelin biogenesis: how the myelin membrane is extended and how it acquires its specific lateral organization. Myelin is a multilayered membrane sheath, which oligodendrocytes wrap around axons to ensure rapid nerve conduction. To investigate molecular mechanisms of myelin membrane growth, we used primary cultures of myelin-producing cells, oligodendrocytes. We have characterized this cellular model system and found that it recapitulates the specific biochemical and morphological characteristics myelin acquires in vivo. Analogous to the situation in vivo, cultured primary oligodendrocytes develop distinct subcellular domains, namely plasma membrane sheets (resembling compact myelin) and processes (resembling non-compact myelin). Interestingly, oligodendrocytes establish these domains in cell culture even without wrapping around axons. To gain insights into myelin membrane expansion mechanisms, we inspected vesicular transport and fusion machineries of oligodendrocytes. We found that biosynthetic organelles, cytoskeleton, exocyst and SNAREs are enriched in the processes and cell bodies of these cells and are almost excluded from the sheets. Further we determined that the vesicular transport pool of compact myelin proteins is also restricted to the processes and cell bodies of oligodendrocytes, whereas these proteins are distributed over the sheets when incorporated into the plasma membrane. Taken together, acquired data indicate that the newly produced myelin membrane is first integrated into the plasma membrane of the processes, but not in the sheets. This raises the question how plasma membrane asymmetry is achieved in oligodendrocytes. We analyzed lateral organization of oligodendroglial membranes and found that, both in culture and in vivo, segregation of the membrane proteins is guided by myelin basic protein (MBP), whose cellular levels are modulated by sphingolipids. In addition, we showed that myelin lipids can phase-separate from the total membrane lipids, implying the possibility for lipid self-organization in myelin. These data suggest a novel mechanism for myelin membrane formation: expansion from the processes into the sheet domains, followed by lateral organization under the control of membrane components (sphingolipids and MBP).
Optimisation of a plasmonic parallel waveguide sensor based on amorphous silicon compounds

This work reports the optimisation of a plasmonic waveguide sensor based on amorphous silicon compounds (a-SiC:H, a-SiN:H or a-SiCN:H) using the FDTD method and modal decomposition. The sensor consists of an array of parallel surface plasmon interferometers with different propagation lengths, each one comprising a thin layer of metal embedded into an amorphous silicon waveguide. In order to reduce the complexity and hardware, we have proposed a structure consisting of an array of parallel surface plasmon interferometers with different propagation lengths, such that at the end of the plasmonic structure the modes can interfere constructively or destructively depending on the refractive index of the sampling medium and the propagation length. The variation of the output intensity at the end of each waveguide element provides a convenient interrogation scheme. In this work we analyse different solutions for splitting the input fundamental mode into the different parallel waveguides, including multi-mode interference structures and directional coupler splitters. By exploring amorphous silicon compounds that can be deposited by Pressure Enhanced Chemical Vapor Deposition (PECVD) at low temperatures, we aim to achieve a low-cost process that is compatible with back-end CMOS processing and wavelengths in the visible to near infrared range.