Miracle-dz/UniFAR-FTUs huggingface dataset:
Contribution of phosphate intrinsic binding energy to the enzymatic rate acceleration for triosephosphate isomerase.

We report that 80% of the enzymatic rate acceleration for the prototypical proton transfer from carbon catalyzed by triosephosphate isomerase can be directly attributed to the remote phosphodianion group of the substrate (R)-glyceraldehyde 3-phosphate, and that the intrinsic binding energy of this functional group in the transition state for enzyme-catalyzed enolization is 14 kcal/mol.
Three-step monoclonal antibody tumor targeting in carcinoembryonic antigen-positive patients.

We describe a method to postlabel, in vivo, biotinylated monoclonal antibodies pretargeted onto tumor deposits when most of the non-tumor-bound antibodies have already been cleared as avidin-bound complexes. The application of this principle to tumor detection by immunoscintigraphy was tested in 20 patients with histologically documented cancer and increased circulating carcinoembryonic antigen levels. One mg of biotinylated anti-carcinoembryonic antigen monoclonal antibody (FO23C5) was administered i.v. (first step). After 3 days, 4-6 mg of cold avidin were injected i.v. (second step), followed 48 h later by 0.2-0.3 mg of a biotin derivative labeled with 111In (2-3 mCi) (third step). No evidence of toxicity was observed. Whole body radioactivity distribution was measured in five patients at various intervals postinjection by the conjugate counting technique. Tumors and metastases were detected in 18 of 19 patients (the remaining patient was a true negative) within 3 h after administration of 111In-biotin by planar or single photon emission tomography imaging. At the time of imaging, tumor/blood pool ratio was 5.5 +/- 3.2, and tumor/liver ratio was 6.7 +/- 3.9. Blood clearance of 111In-biotin was multiexponential, with the fast component having a t1/2 of 5 +/- 3 min. Urinary excretion of radioactivity over 3 h was 63.5 +/- 4.9% of the injected dose. Radioactivity at 3 h was 6.5 +/- 1.8% in blood, 1.6 +/- 0.3% in the kidney, and 2.4 +/- 0.6% in the liver. This approach represents an improvement in immunoscintigraphic techniques for tumor localization. The potential use for radioimmunotherapy is discussed.
Adhesin-receptor interactions in Pasteurellaceae.

The ability of bacteria to adhere to mucosal epithelium is dependent on the expression of adhesive molecules or structures, called adhesins, that allow attachment of the organisms to complementary molecules on mucosal surfaces, the receptors. Important human and animal pathogens are found among the Pasteurellaceae family which includes Haemophilus, Actinobacillus, and Pasteurella organisms. The purpose of this paper is to review the adhesin-receptor systems found in Pasteurellaceae, with an emphasis on recent developments in this specific area. Most of these organisms can employ multiple molecular mechanisms of adherence (or multiple adhesins) to initiate infection. Indeed, a wide variety of adhesins are expressed by members of the Pasteurellaceae, and different proteins (e.g. fimbriae, fibrils, outer membrane proteins) as well as polysaccharides (lipooligosaccharides, lipopolysaccharides, capsular polysaccharides) were clearly shown to play an important role in adherence. In many instances, these adhesins have proved to represent good vaccine candidates. Surprisingly, the receptors on host mucosal surfaces have yet been identified in very few cases.
A novel Monte Carlo approach to hybrid local volatility models

We present in a Monte Carlo simulation framework, a novel approach for the evaluation of hybrid local volatility [Risk, 1994, 7, 18–20], [Int. J. Theor. Appl. Finance, 1998, 1, 61–110] models. In particular, we consider the stochastic local volatility model—see e.g. Lipton et al. [Quant. Finance, 2014, 14, 1899–1922], Piterbarg [Risk, 2007, April, 84–89], Tataru and Fisher [Quantitative Development Group, Bloomberg Version 1, 2010], Lipton [Risk, 2002, 15, 61–66]—and the local volatility model incorporating stochastic interest rates—see e.g. Atlan [ArXiV preprint math/0604316, 2006], Piterbarg [Risk, 2006, 19, 66–71], Deelstra and Rayée [Appl. Math. Finance, 2012, 1–23], Ren et al. [Risk, 2007, 20, 138–143]. For both model classes a particular (conditional) expectation needs to be evaluated which cannot be extracted from the market and is expensive to compute. We establish accurate and ‘cheap to evaluate’ approximations for the expectations by means of the stochastic collocation method [SIAM J. Numer. Anal. , 2007, 45, 1005–1034], [SIAM J. Sci. Comput. , 2005, 27, 1118–1139], [Math. Models Methods Appl. Sci., 2012, 22, 1–33], [SIAM J. Numer. Anal. , 2008, 46, 2309–2345], [J. Biomech. Eng., 2011, 133, 031001], which was recently applied in the financial context [Available at SSRN 2529691, 2014], [J. Comput. Finance, 2016, 20, 1–19], combined with standard regression techniques. Monte Carlo pricing experiments confirm that our method is highly accurate and fast.
Variability of on-treatment platelet reactivity in patients on clopidogrel

Abstract Response to clopidogrel therapy is subject to inter-individual variability. However, data with regard to on-treatment platelet reactivity over time in patients undergoing coronary stenting are scarce. For this prospective observational study, 102 consecutive patients on dual antiplatelet therapy undergoing coronary stenting due to stable coronary artery disease (CAD; n = 29), non ST-elevation acute coronary syndrome (NSTE-ACS; n = 45) and ST-elevation myocardial infarction (STEMI; n = 28) were enrolled. Vasodilator-stimulated phosphoprotein-phosphorylation assay was performed at baseline, as well as 1, 3 and 6 months thereafter. Platelet reactivity index (PRI) measured after 1, 3 and 6 months was lower compared to baseline values (p < 0.001). Variables responsible for reduced response to clopidogrel at baseline (reticulated platelet fraction, simvastatin therapy) and during steady-state phase (body mass index, blood glucose concentrations, cholesterol/HDL-ratio and quality of life score) were different. High on-treatment platelet reactivity (HTPR)-phenotype (cut-off = 50% PRI) within the first month changed in 31% of stable CAD, 33% of NSTE-ACS and 39% of STEMI patients, respectively. HTPR-phenotype in the steady-state phase (month 1 to 6) changed in 45% of stable CAD, 33% of NSTE-ACS and 25% of STEMI patients. Response to clopidogrel and accordingly platelet function might vary over time, especially when a cut-off based approach, is used. There was a significant reduction of on-treatment platelet reactivity within the first month after percutaneous coronary intervention with stenting which was maintained for up to 6 months. Variables associated with reduced response to clopidogrel at baseline and during steady-state phase were different, as the latter mainly reflected an unfavorable metabolic profile, comprising elevated BMI, blood glucose levels as well as cholesterol/HDL-ratio.
Two-eared listening in dynamic situations

Reports of hearing disabilities, particularly those acoustically and perceptually dynamic circumstances, are associated with compromised binaural capacities. Those disabilities that are compromised largely correspond to the areas where benefits of bilateral fitting over unilateral fitting emerge. The singular exception is for disabilities in “Sound quality and naturalness”. When binaural capacities are measured by traditional static masking level difference paradigms, relationships between compromised binaural capacity and self-reports of disability are largely absent. However, when masking level differences are assessed using dynamic parameters that are more representative of the conditions experienced in everyday living then significant associations between binaural capacities and self-reports of disability do emerge. We contend that, for both the self-report domain and the psychoacoustical domain, procedures should be more properly representative of the dynamic environments that listeners are routinely exposed to.
Effect of near adds on the variability of accommodative response in myopic children

Citation information: Sreenivasan V, Irving EL & Bobier WR. Effect of near adds on the variability of accommodative response in myopic children. Ophthalmic Physiol Opt 2011, 31, 145–154. doi: 10.1111/j.1475‐1313.2010. 00818.x
Person Reidentification via Discrepancy Matrix and Matrix Metric

Person reidentification (re-id), as an important task in video surveillance and forensics applications, has been widely studied. Previous research efforts toward solving the person re-id problem have primarily focused on constructing robust vector description by exploiting appearance’s characteristic, or learning discriminative distance metric by labeled vectors. Based on the cognition and identification process of human, we propose a new pattern, which transforms the feature description from characteristic vector to discrepancy matrix. In particular, in order to well identify a person, it converts the distance metric from vector metric to matrix metric, which consists of the intradiscrepancy projection and interdiscrepancy projection parts. We introduce a consistent term and a discriminative term to form the objective function. To solve it efficiently, we utilize a simple gradient-descent method under the alternating optimization process with respect to the two projections. Experimental results on public datasets demonstrate the effectiveness of the proposed pattern as compared with the state-of-the-art approaches.
Comparative effects of oral administration of trimethoprim/sulphadiazine or oxytetracycline on the faecal flora of horses

A study was carried out on the bacteriological faecal flora of horses before and after oral doses of oxytetracycline or trimethoprim plus sulphadiazine. Administration of oxytetracycline was rapidly followed by large increases in counts of coliforms. Bacteroides and Streptococcus species, the disappearance of Veillonella species, the appearance of Clostridium perfringens type A in large numbers and the accumulation of watery fluid in the rectal contents. These changes were not seen following administration of trimethoprim-sulphadiazine and it was concluded that oral treatment of horses with this combination was unlikely to be accompanied by the hazard of inducing colitis.
Enzyme-linked immunosorbent assay for detection of type A streptococcal exotoxin: kinetics and regulation during growth of Streptococcus pyogenes

We describe the detection and quantitation of type A streptococcal exotoxin (erythrogenic toxin, streptococcal pyrogenic exotoxin) by an enzyme-linked immunosorbent assay. This sensitive and specific technique detected microgram amounts of type A exotoxin and was useful for studying the kinetics and regulation of type A exotoxin production during the growth of Streptococcus pyogenes NY5. Maximum production of type A exotoxin was observed during the mid-log phase of growth, similar to the production of other streptococcal extracellular products. When S. pyogenes NY5 was grown at 42 degrees C, decreases in both growth and type A exotoxin production were observed. The results obtained when we studied the influence of nutrient additives and metal ions on the production of type A exotoxin led to the conclusion that none of these factors significantly affected type A exotoxin synthesis and that regulation was constitutive.
An analysis of the temperature effects of fever on the intra-host population dynamics of Plasmodium falciparum

Observations that growth of Plasmodium falciparum in vitro is inhibited by high temperatures have led to hypotheses that malaria fever may influence the parasite population dynamics, regulating parasite density and synchronizing parasite growth. In order to investigate the fever hypotheses, we have developed an age-structured coupled Markov chain model that describes the parasite erythrocyte cycle and its interaction with the host fever response. We estimated the model parameters using data collected from laboratory parasite cultures that were exposed to febrile or normal temperature. Using the experimental parameter values, quantitative predictions were made of the effect of fever in determining the parasite population dynamics. It was concluded from the model behaviour that, during the primary infection of a non-immune host, a typical episode of fever can effect density-dependent regulation of the parasite population, maintaining cycles of parasitaemia and promoting synchronous parasite growth.
Knowledge management adoption in times of crisis

– Organizations are threatened by business crises that may be prevented or lessened if critical knowledge is identified in advance. The purpose of this paper is to enhance the understanding of the role knowledge management (KM) plays in affecting organizational performance during crises. , – A case study of a business crisis was conducted to investigate how KM helped mitigate damage caused by the crisis. , – First, KM plays an important role in crisis management. Second, organizations have different knowledge needs that require the application of different KM strategies at different phases of a business crisis to achieve the best results. Finally, the experience handling crises makes organizations consider their incompetence and thus leads to the generation of organization‐wide learning initiatives which facilitate knowledge acquisition, sharing, and institutionalization. , – The proposed knowledge‐centered crisis management framework can be used by organizations as guidelines to develop their KM strategies based on their concerns during business crises. , – Few prior studies have specifically addressed the role KM plays when encountering business crises. This study focuses on investigating how organizations can apply useful KM strategies based on their knowledge needs and thus achieve desirable crisis management outcomes.
Association of Single Nucleotide Polymorphism in Bone Morphogenetic Protein Receptor 1B (BMPR-1B) Gene with Growth Traits in Chicken

Growth traits are under the control of multiple genes. Understanding the genetic information of related genes is helpful for the selection and breeding course through marker assisted selection. The aim of the current study was to investigate the association of A287G SNP in BMPR-1B gene with growth traits in chicken. A single nucleotide polymorphism was identified in 240 individuals using the PCR-RFLP technique and confirmed by sequencing. The allelic and genotypic frequencies were compared, using the Chisquared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model of statistical software SAS. Three genotypes (AA, AG and GG) were detected in Fayoumi and Rhold Island Red chicken. Sequencing revealed one mutation (287 A→G) in the genotype AA in comparison to the genotype GG. The A287G SNP of BMPR-1B gene was associated significantly with body weight at 2 nd (P=0.022), 3 rd (P=0.034), 4 th (P=0.011), 5 th (P=0.035), 6 th (P=0.001), 7 th (P=0.008) and 8 th (P=0.016) week of age. In conclusion, BMPR-1B gene may be associated with body weight in chicken and may be considered in Marker Assisted Selection program to improve chicken growth performance.
Increase in condom use and decline in HIV and sexually transmitted diseases among female sex workers in Abidjan, Côte d'Ivoire, 1991–1998

Objective To assess clinic- and community-based trends in demographic and behavioral characteristics and clinic-based trends in HIV infection and other sexually transmitted diseases (STD) in female sex workers in Abidjan, Côte d'Ivoire. Design Multiyear cross-sectional study of first-time attenders in Clinique de Confiance, a confidential STD clinic; biannual community-based behavioral surveys. Methods From 1992 to 1998, female sex workers were invited to attend Clinique de Confiance, where they were counseled, interviewed, clinically examined during their first visit and tested for STD and HIV infection. Community-based surveys, conducted in 1991, 1993, 1995, and 1997, interviewed women regarding socio-demographic characteristics and HIV/STD-related knowledge, attitudes and behavior. Results Among female sex workers in Abidjan, there was a trend toward shorter duration of sex work, higher prices, and more condom use. Among sex workers attending Clinique de Confiance for the first time, significant declines were found in the prevalence of HIV infection (from 89 to 32%), gonorrhoea (from 33 to 11%), genital ulcers (from 21 to 4%), and syphilis (from 21 to 2%). In a logistic regression model that controlled for socio-demographic and behavioral changes, the year of screening remained significantly associated with HIV infection. Conclusion The increase in condom use and the decline in prevalence of HIV infection and other STD may well have resulted from the prevention campaign for female sex workers, and such campaigns should therefore be continued, strengthened, and expanded.
Zinc Bells Rang in Jerusalem!

Researchers met to discuss zinc signaling in health and disease. “Oh, Jerusalem of gold, and of light, and of bronze…” goes the popular song. But it was another metal that towered above the Jerusalem landscape during the meeting of the International Society for Zinc Biology (ISZB; http://www.iszb.org/), held at Mishkenot Sha’ananim, a whisper away from the Old City walls. More than 100 scientists gathered on 1 to 5 December 2009 to discuss their research on the biology of this metal. Zinc is a double-edged sword. Zinc supplementation accelerates wound healing and growth and promotes an effective immune response. On the other hand, zinc deficiency leads to growth retardation and impaired learning and memory function, and has been linked to mood disorders. At the cellular level, however, uncontrolled increases in zinc concentrations can lead to neuronal cell death and may be involved in neurodegenerative disorders. Through regulation of various intracellular signaling pathways, zinc can accelerate cell growth and possibly contribute to cancer. However, despite the physiological and clinical importance of this metal, research on the molecular basis of these effects is still in its infancy. The 2009 ISZB meeting provided a venue for investigators working on various zinc-related issues to share their thoughts and ideas and to promote the growth of this field.
Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by the DNA cross‐linking agent cisplatin

DNA double‐strand breaks (DSBs) in chromatin, whether induced by radiation, antitumor drugs, or by apoptosis‐associated (AA) DNA fragmentation, provide a signal for histone H2AX phosphorylation on Ser‐139; the phosphorylated H2AX is denoted γH2AX. The intensity of immunofluorescence (IF) of γH2AX was reported to reveal the frequency of DSBs in chromatin induced by radiation or by DNA topoisomerase I (topo 1) and II (topo 2) inhibitors. The purpose of this study was to further characterize the drug‐induced (DI) IF of γH2AX, and in particular to distinguish it from AA γH2AX IF triggered by DNA breaks that occur in the course of AA DNA fragmentation.
Dietary predictors of the insulin-like growth factor system in adolescent females: results from the Dietary Intervention Study in Children (DISC).

BACKGROUND The insulin-like growth factor (IGF) system is associated with the adult diet and chronic disease. Childhood diet may influence chronic disease through its effect on the IGF system; however, there is limited information describing the dietary predictors of the IGF system in adolescents. OBJECTIVE We examined associations between dietary food intake [fat, protein (animal and vegetable), carbohydrate, lactose, dietary fiber, calcium, zinc, and sodium] and serum IGF-I, IGF binding protein 1 (IGFBP-1), IGF binding protein 3 (IGFBP-3), and the IGF-I:IGFBP-3 molar ratio in adolescent females. DESIGN One hundred fifty-nine adolescent females in the Dietary Intervention Study in Children (age range: 14-18 y; 0.2-6.3 y postmenarche) were included. The dietary intake was assessed via three 24-h dietary recalls. IGF-related biomarkers were determined by using radioimmunoassays. Associations between dietary intakes and biomarkers were assessed with Pearson's correlations and multivariable linear regression. Dietary intakes and biomarkers were logarithmically transformed; thus, beta coefficients represented percentages. RESULTS In analyses adjusted for energy, age, and time since menarche, significant correlations (P < 0.05) were as follows: IGF-I with total protein, lactose, calcium, and sodium; IGFBP-3 with total fat (inverse), lactose, fiber, and calcium; IGF-I/IGFBP-3 with lactose and calcium; and IGFBP-1 with vegetable protein. In multivariable analyses, significant predictors of IGF-I were energy (beta = 0.14, P < 0.05) and calcium (beta = 0.14, P < 0.01), the significant predictor of IGFBP-3 was calcium (beta = 0.07, P < 0.05), and significant predictors of IGFBP-1 were vegetable protein (beta = 0.49, P < 0.05) and body mass index-for-age percentile (beta = -0.01, P < 0.001). CONCLUSION This study provides evidence that dietary intake affects IGF-related biomarkers-particularly elevated calcium with IGF-I and IGFBP-3 and elevated vegetable protein with IGFBP-1-and, to our knowledge, is novel in reporting these associations in adolescent females. The Dietary Intervention Study in Children was registered at clinicaltrials.gov as NCT00000459.
Successive learning of linear discriminant analysis: Sanger-type algorithm

Linear discriminant analysis (LDA) is applied to broad areas, e.g. image recognition. However, successive learning algorithms for LDA are not sufficiently studied while they have been well established for principal component analysis (PCA). A successive learning algorithm which does not need N/spl times/N matrices has been proposed for LDA (Hiraoka and Hamahira, 1999, and Hiraoka et al., 2000), where N is the dimension of data. In the present paper, an improvement of this algorithm is examined based on Sanger's (1989) idea. By the original algorithm, we can obtain only the subspace which is spanned by major eigenvectors. On the other hand, we can obtain major eigenvectors themselves by the improved algorithm.
N myristylation of the human immunodeficiency virus type 1 gag polyprotein precursor in Saccharomyces cerevisiae

A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.
A comparison of two methods of value measurement.

Category rating and ratio estimation were used by 621 staff respondents to scale the importance (value) of 16 possible outcomes of releasing psychiatric patients or keeping them in the hospital. Stevens's claim that the relationship between the two methods is intermediate between linear and logarithmic was confirmed for seven of the eight occupational groups-but not for psychologists. The two methods yielded essentially identical answers to the substantive survey questions.