Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytomegalovirus glycoprotein B sequence variation among Japanese bone marrow transplant recipients.

Glycoprotein B (gB) of human cytomegalovirus (CMV) is the major target protein of neutralizing antibodies, and four variant types are known. A previously reported polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method was modified by eliminating the cell culture step and amplifying a CMV fragment (corresponding to a.a. 441-450 of gB) by nested PCR. DNA was extracted from the sera of 27 pediatric bone marrow transplant recipients and subjected to nested PCR. Of the samples, 20 yielded PCR products, and the gB type was determined by RFLP. Of the 20 patients, 4 (20%) had gB type 1 (Towne type), 15 patients (75%) had gB type 2 (AD 169 type), and 1 patient (5%) had gB type 3. Previous studies showed that gB type was most common among Caucasians [Chou and Dennison: J Infect Dis 1991;163:1229-1234; Fries et al: J Infect Dis 1994;169:769-774]. Thus, gB genotypes seem to be distributed differently in Caucasians and Japanese. Further, nucleotide sequence analysis of the amplified region revealed that all the type 2 viruses had the same amino acid sequences. The type 3 sample had a novel amino acid substitution at position 498. Of the type 1 samples, 3 had amino acid substitutions in various positions: 1 sample at position 493, 1 sample at position 447 and 1 sample at position 452, 493 and 498.
An approach to analyze the impact of DDOS attack on mobile cloud computing

Mobile ad-hoc network is form of wireless networks recognized with the purpose of provisional communication with no additional infrastructure. Open nature communication make is prone for several security threats such as resource draining attacks or routing exhaustion. Security is the major concern to maintain the trust on communication or network concert. Cloud computing is broad and diverse technology. Clients are allowed to share their resource and infrastructure as service with no major hurdles. It helps to store large amount of data and make remote transactions with public or private network infrastructure. Cloud computing using MANET is different joint venture of new era help to establish resource sharing with ad-hoc nature. Authors investigate that illegal access and damage of resources pathetic and can damage equivalent as illegal access to data. Study also observes that resource draining is completely independent process and doesn't lie on vulnerabilities of routing protocols. Denial of Service attack and Distributed Denial of Service attack is kind of resource draining purposely deployed to degrade the performance by exhausting the resource. This paper aims to make survey of all issues relates this security concern on MANET and Cloud computing.
Novel Therapeutic Indications of Antidepressant Drugs

This review will concentrate on the pharmacotherapy of bulimia nervosa (BN). The commonly presenting eating are characterised in figure 1 below and the DSM-IV criteria for BN (table 1) are also given. Although drugs have been widely used in the treatment of anorexia nervosa, there is no convincing evidence of their efficacy. Binge eating disorder has only recently been described and as yet there are no controlled trials of drug treatment. As far as BN is concerned, a number of different classes of drugs have been studied including anticonvulsants, appetite suppressants, opiate antagonists, lithium and antidepressants. The latter have been most extensively evaluated, and the initial rationale for their use was provided by the observation of an association between BN and depressive symptomatology [Russell, 1979; Abraham and Beumont, 1982; Hudson et al., 1982; Gwirtsman et al., 1983; Walsh et al., 1985; Swift et al., 1986; Cooper and Fairburn, 1986; Levy et al., 1989]. There are however a number of reasons why drug treatment might be considered in BN, and these are summarised in table 2. Fig. 1. Relationship of eating disorders to each other. AN = Anorexia nervosa; BED = binge eating disorder. ▲
Experimental Approaches to White Matter Disease

The study of the cerebral white matter (WM) in experimental models of hypoxia and ischemia has been for a long time neglected, the interest of investigators being focused on the response of the neuronal population. The increasing frequency with which modern neuroimaging techniques disclose alterations of the WM of possibly vascular origin has called some attention to other components of the cerebral parenchyma, namely oligodendrocytes and myelinated axons. The studies here reviewed show that it is possible to investigate the WM alterations in appropriate models of cerebral ischemia. In rodents, chronic ischemia produces extracellular fluid accumulation in the WM, moderate loss of oligodendroglial cells and reactive astrogliosis, all of which contribute to the rarefied appearance under the light microscope. These changes are similar to those found in many patients with leukoencephalopathy of possibly vascular origin. The results of experiments using a rat model of focal brain ischemia show that oligodendrocytes and myelinated fibers are extremely sensitive to ischemic insults with alterations of the subcortical WM detected at a very early time. Opportune modifications of these animal models may represent a valuable source of information on the pathogenesis and pathophysiology of human vascular disease of the WM.
Exponentially Increased Thermal Resistance of Salmonella spp. and Enterococcus faecium at Reduced Water Activity

ABSTRACT Salmonella spp. exhibit prolonged survivability and high tolerance to heat in low-moisture foods. The reported thermal resistance parameters of Salmonella spp. in low-moisture foods appear to be unpredictable due to various unknown factors. We report here that temperature-dependent water activity (aw, treatment temperature) plays an important role in the sharply increased thermal resistance of Salmonella enterica serovar Enteritidis PT 30 and its potential surrogate Enterococcus faecium NRRL B-2354. In our study, silicon dioxide granules, as carriers, were separately inoculated with these two microorganisms and were heated at 80°C with controlled relative humidity between 18 and 72% (resulting in corresponding aw,80°C values for bacteria between 0.18 and 0.72) in custom-designed test cells. The inactivation kinetics of both microorganisms fitted a log-linear model (R2, 0.83 to 0.97). Reductions in the aw,80°C values of bacterial cells exponentially increased the D80°C (the time needed to achieve a 1-log reduction in a bacterial population at 80°C) values for S. Enteritidis and E. faecium on silicon dioxide. The log-linear relationship between the D80°C values for each strain in silicon dioxide and its aw,80°C values was also verified for organic wheat flour. E. faecium showed consistently higher D80°C values than S. Enteritidis over the aw,80°C range tested. The estimated zaw (the change in aw,80°C needed to change D80°C by 1 log) values of S. Enteritidis and E. faecium were 0.31 and 0.28, respectively. This study provides insight into the interpretation of Salmonella thermal resistance that could guide the development and validation of thermal processing of low-moisture foods. IMPORTANCE In this paper, we established that the thermal resistance of the pathogen S. Enteritidis and its surrogate Enterococcus faecium, as reflected by D values at 80°C, increases sharply with decreasing relative humidity in the environment. The log-linear relationship between the D80°C values of each strain in silicon dioxide and its aw,80°C values was also verified for organic wheat flour. The results provide new quantitative insight into the way in which the thermal resistance of microorganisms changes in low-moisture systems, and they should aid in the development of effective thermal treatment strategies for pathogen control in low-moisture foods.
Optimizing NBE PK/PD assays using the Gyrolab Affinity Software; conveniently within the bioanalyst's existing workflow.

AIM The fully automated microfluidics-based Gyrolab is a popular instrument for the bioanalysis of protein therapeutics; requiring minimal sample and reagent volumes. Gyros offers affinity software for determining binding affinity in solution using a high-throughput method and miniaturized reactions. RESULTS Using this affinity software, multiple CTGF-targeting reagents were characterized on the Gyrolab after <100% target coverage was seen in a cynomolgus pharmacokinetic/PD study dosed with anti-CTGF antibodies. The results uncovered magnitude differences in binding affinities between the dosed antibody, target and assay reagents. CONCLUSION The binding affinity values were used to investigate reduced target coverage and results highlight potential of the affinity software for incorporation into the bioanalyst's existing Gyrolab workflow for characterizing reagents and optimizing pharmacokinetic/PD bioanalytical assays.
Cytokine-Mediated Growth Hormone Release from Cultured Ovine Pituitary Cells

Previous studies have demonstrated that intravenous lipopolysaccharide (LPS) will increase concentrations of growth hormone (GH). One possible explanation for this may reside in the response of the pituitary to specific cytokines. This study sought to determine the effects of recombinant bovine tumor necrosis factor α (TNF), recombinant ovine (ro) interleukin-1α (IL-1α), roIL-1β, ro interleukin-2 (IL-2), and ro γ-interferon (INT) on GH release from cultured sheep pituitary cells. Sheep were sacrificed and pituitary cells cultured in DMEM with 10% fetal bovine serum for 3 days. On day 4, cells were washed and serum-free DMEM added to cells. IL-1α and IL-1β were used at 0.2, 2 and 20 ng/ml and the remaining cytokines at 2, 20 and 200 ng/ml. Neither IL-2 nor INT had effects on basal or on GH-releasing hormone (GRH)-stimulated GH release. TNF inhibited GRH-stimulated GH release (p < 0.05). Both IL-1α and IL-1β stimulated GH release from cultured pituitary cells at all doses tested (p < 0.01). Neither IL-1α nor IL-1β had an effect on GRH-stimulated GH release. IL-1 effects were inhibited by H-89 (p < 0.05; a protein kinase A inhibitor) and by nifedipine (p < 0.05; a calcium channel blocker). Both of these mechanisms are central signal transduction mechanisms mediating GRH-stimulated GH release. IL-1-stimulated GH release is partially inhibited (p < 0.05) by lipoxygenase pathway blockers. Phorbol myristate acetate downregulation of protein kinase C did not alter IL-1-stimulated GH release. IL-1β increased the content of both GH and GH mRNA in cultured sheep pituitary cells. We conclude that IL-1 produces a strong stimulus to GH release, which is mediated by calcium entry and protein kinase A activation. IL-1 also activates lipoxygenase pathways. This latter pathway as well as calcium entry were shown to mediate LPS stimulation of GH release from cultured pituitary cells. The similarity between IL-1 and LPS signal transduction suggests that LPS may activate pituitary production of IL-1 to produce the stimulus to GH. The lack of inhibitory effects of INT, TNF and IL-2 as opposed to what is seen in the rat may suggest a partial mechanism to explain the different effects of LPS on GH release between sheep and that seen in cattle and rats.
A Framework for Scalable TSV Assignment and Selection in Three-Dimensional Networks-on-Chips

3D integration can greatly benefit future many-cores by enabling low-latency three-dimensional Network-on-Chip (3D-NoC) topologies. However, due to high cost, low yield, and frequent failures of Through-Silicon Via (TSV), 3D-NoCs are most likely to include only a few vertical connections, resulting in incomplete topologies that pose new challenges in terms of deadlock-free routing and TSV assignment. The routers of such networks require a way to locate the nodes that have vertical connections, commonly known as elevators, and select one of them in order to be able to reach other layers when necessary. In this paper, several alternative TSV selection strategies requiring a constant amount of configurable bits per router are introduced. Each proposed solution consists of a configuration algorithm, which provides each router with the necessary information to locate the elevators, and a routing algorithm, which uses this information at runtime to route packets to an elevator. Our algorithms are compared by simulation to highlight the advantages and disadvantages of each solution under various scenarios, and hardware synthesis results demonstrate the scalability of the proposed approach and its suitability for cost-oriented designs.
Promoting proliferation and differentiation of BMSCs by green tea polyphenols functionalized porous calcium phosphate

Abstract In this article, we proposed a facile protocol to functionalize porous calcium phosphate ceramics (PCPC) using dietary tea polyphenols (TP). TP molecules was attracted and anchored by Ca2+ ions from the surface of CPC. These TP molecules modulated the nucleation and crystallization of calcium phosphate nanorods assemblies on the surface of PCPC. Our results prove that these calcium phosphate nanorods assemblies accompanies functional groups of TP make PCPC/TP effectively promote proliferation and differentiation of bone mesenchymal stem cells (BMSCs). We inferred that these calcium phosphate nanorods assemblies might change the surface microenvironment of PCPC, which is critical to promote the proliferation and differentiation of BMSCs. Compared with naked PCPC, PCPC/TP obviously increased BMP2, ErK/MAPK and JNK/MAPK level and mineralization capacity of cells (ALP level).
Implementation of the Consultation-liaison Model in Quebec and its Impact on Primary Care Providers

Background: With increasing resource scarcity in specialized mental healthcare, collaborative care models have been advanced in order to strengthen primary mental healthcare. The consultation-liaison model was introduced in conjunction with sweeping reform of the Quebec (Canada) healthcare system. Respondent-psychiatrists were hired to provide consultation and support to both general practitioners (GP) working in medical clinics and primary care MH teams. This study evaluates the impact of respondent-psychiatrists on the capacity of GPs and primary care teams to diagnose and treat MH disorders, and identifies barriers and facilitators to their deployment within the consultation-liaison model. Methods: Eleven local networks provided the setting. Data collection included structured questionnaires completed by 29 respondent-psychiatrists and 33 managers, and semi-structured interviews with 102 network stakeholders. Mixed methods were employed, triangulating data from questionnaires, interviews and network-related documents. Results: Respondent-psychiatrists collaborated more effectively with MH primary care teams than with GPs. Barriers to implementation of respondent-psychiatrists were identified, including poor comprehension of the model, inadequate provincial coordination, resistance from psychiatrists and GPs, lack of financial incentives for GPs, little knowledge of GP and patient needs in primary care, and physical distance between GPs and respondent-psychiatrists. Conclusion: Although respondent-psychiatrists positively impacted MH primary care teams, the consultation-liaison model as implemented did not fully meet needs among GPs who treat most MH problems. Implementation called for better model indicators and provincial-level coordination. A more comprehensive model of collaborative care including integrated MH teams and psychosocial care managers might optimize the use of GPs in primary mental healthcare.
Prologue: Reading Comprehension Is Not a Single Ability.

Purpose In this initial article of the clinical forum on reading comprehension, we argue that reading comprehension is not a single ability that can be assessed by one or more general reading measures or taught by a small set of strategies or approaches. Method We present evidence for a multidimensional view of reading comprehension that demonstrates how it varies as a function of reader ability, text, and task. The implications of this view for instruction of reading comprehension are considered. Conclusion Reading comprehension is best conceptualized with a multidimensional model. The multidimensionality of reading comprehension means that instruction will be more effective when tailored to student performance with specific texts and tasks.
Parallel Sorting with Dynamic Load-Balancing in Stochastic Environments

Parallel algorithms and specifically parallelism in sorting algorithms as a symbolic application have been intensively studied. Some algorithms are available for parallel sorting by dedicated or homogenous processors. Dedicated processors for sorting are rarely a practice of real world. This paper introduces an algorithm for parallel sorting on processors that are not only utilized for sorting but also may run a multitasking OS and perform other tasks concurrently. This multitasking leads to stochastic behaviors and virtual speeds of the processors felt by the processes including the sorter processes. An agent framework is used to simulate a multiprocessor environment. The results show that the proposed algorithm performs better load balancing between processors than previous algorithms in stochastic speed-variant environments. Key-words: Parallel sorting, Dynamic load-balancing, Overpartitioning, Stochastic environments
Effects of induced sad mood on facial emotion perception in young and older adults

ABSTRACT Older adults perceive less intense negative emotion in facial expressions compared to younger counterparts. Prior research has also demonstrated that mood alters facial emotion perception. Nevertheless, there is little evidence which evaluates the interactive effects of age and mood on emotion perception. This study investigated the effects of sad mood on younger and older adults’ perception of emotional and neutral faces. Participants rated the intensity of stimuli while listening to sad music and in silence. Measures of mood were administered. Younger and older participants’ rated sad faces as displaying stronger sadness when they experienced sad mood. While younger participants showed no influence of sad mood on happiness ratings of happy faces, older adults rated happy faces as conveying less happiness when they experienced sad mood. This study demonstrates how emotion perception can change when a controlled mood induction procedure is applied to alter mood in young and older participants.
Planning the Literature Review.

Disclaimer: The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Uniformed Services University of the Health Sciences, the Department of the Navy, the Department of Defense, or the U.S. Government. References: 1. Maggio LA, Sewell JL, Artino AR. The literature review: A foundation for high-quality medical educational research. J Grad Med Educ. 2016;8:279–285. 2. Boyer EL. Scholarship Revisited: Priorities of the Professoriate. New York, NY: John Wiley & Sons; 1990. 3. Hofmeyer A, Newton M, Scott C. Valuing the scholarship of integration and the scholarship of application in the academy for health sciences scholars: Recommended methods. Health Res Policy Syst. 2007;5:5. 4. Bordage G. Reasons reviewers reject and accept manuscripts: The strengths and weaknesses in medical education reports. Acad Med. 2001;76:889–896. 5. Maggio LA, Tannery NK, Kanter SL. AM Last Page: How to perform an effective database search. Acad Med. 2011;86:1057. Author contact: justin.sewell@ucsf.edu A literature review provides a synthetic summary and synthesis of what is known and unknown regarding a scholarly topic.1 A rigorous literature review may encourage the use of appropriate methods, enhances generalizability, places research within a broader context, accelerates progress in health professions education, and improves the likelihood of publication.2–4 This Last Page highlights the steps for planning and executing a literature review within health professions education.
Design of heading control system for USV based on MOOS-IvP

This paper presents a design scheme of control system for a small fixed double propulsion unmanned surface vehicle (USV). The system is based on MOOS-IvP star topology, with distributed and modular features. The system integrates the sensor module, the propeller module, the data recording module and the control module. The Autonomous Navigation of USV is achieved successfully by using the heading control method based on PID. Simulink simulation and lake experiment verifies its effectiveness.
Body-part tracking from partial-view depth data

This paper presents a high-accuracy body-part tracking algorithm, capable of achieving efficient human motion analysis from partial view depth-data, suitable for deployment in real-life applications. The algorithm uses a consumer-grade depth camera for data input and combines a discriminative body part estimator along with a generative tracker, utilizing a realistic human body model, in order to track individual body limbs in short camera-distance, partial-view scenarios. Additionally, a shape adaptation feature is also introduced in order to further morph the human model based on the observations. The implementation is tested in a lower-body limbs tracking scenario, achieving promising accuracy and performance on consumer-grade hardware. Moreover, a lower-body motion dataset is also provided, consisting of 16 real-world sequences using automatic ground-truth annotations from a commercial motion capture system.
Parental Educational Attainment and Adult Offspring Personality: An Intergenerational Life Span Approach to the Origin of Adult Personality Traits

Why do some individuals have more self-control or are more vulnerable to stress than others? Where do these basic personality traits come from? Although a fundamental question in personality, more is known about how traits are related to important life outcomes than their developmental origins. The present research took an intergenerational life span approach to address whether a significant aspect of the childhood environment—parental educational attainment—was associated with offspring personality traits in adulthood. We tested the association between parents’ educational levels and adult offspring personality traits in 7 samples (overall age range 14–95) and meta-analytically combined the results (total N > 60,000). Parents with more years of education had children who were more open, extraverted, and emotionally stable as adults. These associations were small but consistent, of similar modest magnitude to the association between life events and change in personality in adulthood, and were also supported by longitudinal analyses. Contrary to expectations, parental educational attainment was unrelated to offspring Conscientiousness, except for a surprisingly negative association in the younger cohorts. The results were similar in a subsample of participants who were adopted, which suggested that environmental mechanisms were as relevant as shared genetic variants. Participant levels of education were associated with greater conscientiousness, emotional stability, extraversion, and openness and partially mediated the relation between parent education and personality. Child IQ and family income were also partial mediators. The results of this research suggest that parental educational attainment is 1 intergenerational factor associated with offspring personality development in adulthood.