Miracle-dz/UniFAR-FTUs huggingface dataset:
c-myc reverses neu-induced transformed morphology by transcriptional repression

Amplification or overexpression or both of either the c-myc or the human neu (C-erbB-2) gene are common events in many primary human tumors. Coamplification or overexpression or both of both genes have been reported in some breast cancers. The possibility of cooperation between the c-myc and the normal rat neu (c-neu) genes in transforming cells was examined. Surprisingly, the expression of c-myc in B104-1-1 cells, and activated rat neu oncogene (neu*)-transformed NIH 3T3 line, resulted in morphologic reversion. This reversion was found to be a consequence of a transcription-repressive action of c-myc on the neu gene via a 140-bp fragment on the neu gene promoter. The effective concentration of a positive factor(s) interacting with this fragment seemed to be lowered by the expression of c-myc. Our findings lend support to arguments concerning the long-suspected function of c-myc as a transcriptional modulator. They also imply that an oncogene such as c-myc, or possibly the rapidly explored class that encodes transcription factors, under certain conditions may act to reverse a transformed phenotype that is induced by another oncogene instead of contributing positively towards the transformation process. Therefore, the activity of an oncogene may depend on the environment in which it is expressed. In addition, we may have identified the neu gene as a cellular target gene of negative regulation by c-myc.
Methylphenidate Misuse in Adults: Survey of 414 Primary Care Physicians in Germany and Comparison with the Literature

Objectives: This paper deals with the methylphenidate (MPH) misuse by adults in Germany. Results of a survey among primary care physicians/internists have been supplemented by a comparison with the literature. Methods: In the period from October 5-20, 2015, a survey was sent to 414 primary care physicians/internists in four German cities (n = 10 were undeliverable). The response rate was 58% (n = 235). 34 original works on MPH abuse worldwide were found in the literature and are used in the analysis of the present data situation. The literature published before November 9, 2015 is considered in this paper. Results: 14% of the doctors who took part in the survey said that they had been asked for MPH prescriptions without any medical indication. The most frequent reason given for the request (42%) was adult attention deficit/hyperactivity disorder (ADHD) not verified by documents. According to the comparison with the literature, university students had a lifetime prevalence of MPH misuse ranging from 0.8% to 16.6% and school children had a misuse rate of 4.0%. In the civilian US population, the misuse rate was 4.2%. Among patients in possession of a current MPH prescription due to a diagnosis, the lifetime prevalence was 29% and among adolescents with suspected alcohol and/or drug problems 20%. Conclusions: MPH misuse is a major problem which has not been studied sufficiently. MPH misuse not only plays a role in the field of psychiatry, but also in other disciplines. Misuse particularly following a therapeutic prescription should be taken into account.
The Assembly of Protein Oligomers — Old Stories and New Perspectives with Graph Theory

Proteins are biological entities made of a chain of amino acids bound to one another in a specific order, called the primary structure or the amino acid sequence of the protein. Based on the sequence and the environment, the protein acquires a tridimensional shape called tertiary structure (3D-structure), conformation or fold, suitable for its biological function. The func‐ tional shape is the native structure of the protein. The set of reactions leading to the native structure is the folding of the protein. The vast majority of proteins are oligomers which function only after the association of several copies of their chains. Homo-oligomers have chains with identical sequences and hetero-oligomers have chains with different sequences. The number of associated chains defines the quaternary structure of the oligomer, or its stoichiometry [1]. According to the Protein Database (PDB) where all known 3D structures of proteins are stored, the most observed quaternary structure in all taxa is the dimer (Fig. 1A). A taxon is a set of living organisms grouped because of some shared characteristics. Besides dimers, there exists a large variety of assemblies in terms of quaternary structures and point group symmetries (Fig. 1). In forming a protein oligomer, subunit association has to be considered in addition to folding.
A design strategy for improving adaptive conjoint analysis

Purpose Adaptive conjoint analysis (ACA) is a market research methodology for measuring utility in business-to-business and customer studies. Based on partial profiles, ACA tailors an experiment’s design to each respondent depending on their previously stated preferences, ordered in a self-assessment questionnaire. The purpose of this paper is to describe advantages and disadvantages of using a partial-profile randomised experiment, the usual system, and to propose a new design strategy for arranging profiles in blocks that improve its performance. Design/methodology/approach The authors propose a comparison between their design with the commonly used designs, as random designs and the so-called “mirror image”, in their resolution capacity for the estimations of main factors and two-factor interactions with the lowest number of profiles. Findings Comparing the proposed design over the other two designs highlights certain aspects. The proposed design guarantees more estimation for each experiment than the others and allows the researcher to tailor the design to his or her goals. The authors’ procedure will help researchers to determine an experiment’s resolution capacity before carrying it out, as well as to estimate main factors and two-factor interactions alike. Originality/value The authors propose a new design strategy for arranging the profiles in blocks for improving the performance of ACA. This proposal is based on the use of a full-profile approach in which profiles are arranged in two-level factorial designs in blocks of two, and the levels of each factor are codified vectorially.
Brazil Numerical Simulation of Two-Dimensional Complex Flows around Bluff Bodies Using the Immersed Boundary Method

This paper presents a two-dimensional numerical sim ulation of flows around different bluff bodies, at Re = 100 and 200, using the Immers ed Boundary (IB) method, as a sequence of a previous work. The force density term r quired by the IB method is obtained with the Virtual Physical Model (VPM). Simulations were carried out for two circular cylinders of different diameter in tandem, two cyli nders of the same diameter in tandem and two cylinders placed in side by side arrangemen t. The configurations of seven cylinders in a ‘V’ arrangement, for angles of 40o ≤ α ≤ 180o, were also simulated. A configuration of 23 different bluff bodies, represe nting a transverse cut in a central tower of an offshore structure, has been also simulated a n the results were compared with a single compact square, of equivalent size. The Stro uhal number, the drag and the lift coefficients were also calculated. The Strouhal num ber is calculated using the Fast Fourier Transform (FFT) of the lift coefficient tem poral distribution. Visualization of the vorticity and pressure fields and the streamlines a re presented for each simulation showing the flow dynamics and patterns. It was poss ible to verify that the IB method with VPM is a powerful methodology to simulate flows in the presence of complex geometries.
Social network and blood pressure: a population study.

&NA; The relationship between social network and blood pressure was assessed in a cross‐sectional, random sample of 1409 white adults aged 20 to 70 years studied in Buffalo in 1961. Higher total social network scores were associated with lower systolic and diastolic blood pressure in both males and females. Considering the components of social network separately, increased size of household and greater participation in clubs were associated with lower systolic blood pressure for males, with the trend in the same direction for women, though not reaching statistical significance. Among females, the only social network component showing significant linear trend was the number of siblings, with increased number of siblings being associated with lower systolic and diastolic blood pressure. Religious service attendance and marital status were not associated with blood pressure in this population.
Indirect Current Feedback Instrumentation Amplifier with a Common Mode Input Range That Includes the Negative Rail

An instrumentation amplifier is presented which can handle common-mode voltages that extend 200 mV below the negative supply. The extended range is combined with a good common-mode rejection (91 dB) and good accuracy (0.1%) without the need for on-chip trimming. This has been achieved by the use of a simple PNP input stage with indirect current feedback. The output voltage can reach the negative supply. The offset voltage is 0.3 mV, the noise voltage is 30 nV/¿Hz. The circuit can work at a supply voltages down to 2.5 V and the quiescent current is 240 ¿A. The instrumentation amplifier has been integrated in a semi-custom bipolar process.
A parametric study of the destruction efficiency of Bacillus spores in low pressure oxygen‐based plasmas

The destruction of Bacillus spores in oxygen‐based plasmas sustained in the millitorr pressure range has been studied as functions of various biological and plasma parameters, namely Bacillus species, surface concentration of spores, treatment temperature, and gas composition. In an oxygen plasma, Bacillus stearothermophilus appears less plasma‐resistant than the other spores tested. Oxygen, H2O2 and chiefly CO2 plasmas are clearly shown to be much more efficient in destroying Bacillus subtilis spores than pure argon plasma. The bacterial surface concentration on the spore carriers and the treatment temperature also lead to significant variations in the destruction efficiency of spores when using CO2 plasma.
Robot visual sliding mode servoing using SIFT features

To solve the robot visual servoing problem in the natural scene, a robot visual sliding servoing method using SIFT features in the framework of epipolar-geometry-based visual servoing is proposed. Firstly, corresponding image feature points from the current image feature and the desired image are obtained by SIFT algorithm; Then the features error of the whole robot visual servoing system are calculated according to the corresponding points; Finally, considering characteristics of epipolar geometry for robot visual servoing system, the rotation sliding mode controller and translation sliding mode controller are sequentially designed to calculate the rotation movement and translation movement of the robot end-effector. Visual positioning experiments on a 6DOF industrial robot demonstrate that the proposed method can effectively locate an object in the natural scene.
Regulation of white and opaque cell‐type formation in Candida albicans by Rtt109 and Hst3

How different cell types with the same genotype are formed and heritability maintained is a fundamental question in biology. We utilized white‐opaque switching in Candida albicans as a system to study mechanisms of cell‐type formation and maintenance. Each cell type has tractable characters, which are maintained over many cell divisions. Cell‐type specification is under the control of interlocking transcriptional feedback loops, with Wor1 being the master regulator of the opaque cell type. Here we show that deletion of RTT109, encoding the acetyltransferase for histone H3K56, impairs stochastic and environmentally stimulated white‐opaque switching. Ectopic expression of WOR1 mostly bypasses the requirement for RTT109, but opaque cells lacking RTT109 cannot be maintained. We have also discovered that nicotinamide induces opaque cell formation, and this activity of nicotinamide requires RTT109. Reducing the copy number of HST3, which encodes the H3K56 deacetylase, also leads to increased opaque formation. We further show that the Hst3 level is downregulated in the presence of genotoxins and ectopic expression of HST3 blocks genotoxin induced switching. This finding links genotoxin induced switching to Hst3 regulation. Together, these findings suggest RTT109 and HST3 genes play an important role in the regulation of white‐opaque switching in C. albicans.
Crop Simulation Growth Model in Cassava

Abstract: Field experiment was conducted at Veterinary College and Research Institute Farm, Namakkal during 2002 to find out the effect of forage intercropping and organic manures on the growth and growth rate of cassava. The popular hybrid of cassava H 226 was tried as test crop. Three intercropping systems viz., Sole cassava, Cassava + maize (var. African tall) and Cassava + cowpea (var. CO 5) and six organic manurial treatments viz., FYM (25 t haG ), Poultry manure (10 t haG ), Composted poultry manure 1 1
[ADHD difficult to diagnose in adults. ASRS v1.1 Self-Report Scales valuable help--now translated to Swedish].

Evidence shows that symptoms and difficulties related to Attention Deficit Hyperactivity Disorder (ADHD) can continue into adulthood. ADHD is often complicated by the presence of comorbid conditions including mood and anxiety disorders, substance abuse, and antisocial disorders. We present a brief overview of ADHD in adulthood and the Swedish translation of the Adult ADHD Self-Report Scales (ASRS). We describe the current use of the ASRS in screening studies and in clinical settings in Sweden.
Correlation of docking energies with spectroscopic kinetic assays of potential xanthine oxidase substrates

Here we present a docking model that ranks compounds according to their potential effectiveness as a potential substrate or inhibitor. We utilize xanthine oxidase (XO), a multi-cofactor oxido-reductase which converts hypoxanthine to xanthine and xanthine to uric acid. During the reductive half reaction, electrons flow from the molybdopterin, to each of two Fe/S centers, and finally to FAD. During the oxidative half reaction, electrons are passed from the FAD to O2. Under ideal physiological conditions, this reduction of oxygen generates H2O2 and, under multiple turnover conditions, superoxide in amounts which is regulated by catalase and superoxide dismutase. Utilizing computer modeling predictions of the docking orientations and energies of a group of purine based structures was selected. Correlating computer estimations with steady state kinetic data, a rapid screening process for inhibittor prediction was highlighted. This method allows educated selection of likely inhibitors, thereby decreasing the time and supplies required to complete a traditional kinetic analysis screening. Results demonstrate the functionality and reliability of this method and have proven particularly useful in understanding binding orienttations or poses of each compound.
Activity-sensitive flip-flop and latch selection for reduced energy

This article presents new techniques to evaluate the energy and delay of flip-flop and latch designs and shows that no single existing design performs well across the wide range of operating regimes present in complex systems. We prepose the use of a selection of flip-flop latch designs, each timed for different activation patterns and speed requirements. We illustrate the use of our technique on a pipelined MIPS processor datapath running SPECint95 benchmarks, where we reduce total flip-flop and latch energy by 60% without increasing cycle time.
Punishing drug addicts who have babies: women of color, equality, and the right of privacy.

Part of the Civil Rights and Discrimination Commons, Constitutional Law Commons, Criminal Law Commons, Fourteenth Amendment Commons, Gender and Sexuality Commons, Inequality and Stratification Commons, Law and Gender Commons, Law and Race Commons, Law and Society Commons, Law Enforcement and Corrections Commons, Legal History Commons, Privacy Law Commons, Race and Ethnicity Commons, and the Social Control, Law, Crime, and Deviance Commons
Oral administration of a decaffeinated green tea (Camellia sinensis) extract did not alter urinary 8‐epi‐prostaglandin F2α, a biomarker for in‐vivo lipid peroxidation

Oxidative stress is involved in the pathogenesis of numerous chronic human diseases. The objective of this study was to determine whether administration of a decaffeinated green tea extract providing 844 mg flavonoids daily reduced the urinary excretion of 8‐epi‐prostaglandin F2α (8‐epi‐PGF2α), a product of lipid peroxidation in cellular membranes and of low‐density lipoprotein (LDL). Nine healthy male and female subjects were studied at baseline and after 14 days of green tea supplementation. Analysis of urinary 8‐epi‐PGF2α was performed using immunoaffinity extraction‐gas chromatography‐negative ion chemical ionization‐mass spectrometry (GC‐NICI‐MS). Urinary 8‐epi‐PGF2α concentrations were 0.286±0.120 nmol (mmol creatinine)−1 at baseline and 0.244±0.177 nmol mmol−1 creatinine after green tea supplementation. There were no significant differences in the excretion of urinary 8‐epi‐PGF2α after treatment with green tea. We conclude that 14 days of green tea supplementation did not significantly alter in‐vivo lipid peroxidation.
Synthesis and Spectral Study of New Azo - Azomethine Dyes and its Copper (II) Complexes Derived from Resorcinol, 4-Aminobenzoylhydrazone and 4-Amino antipyrine

Four new azo dyes L1, L2, L3 and L4, were prepared by linking benzaldehyde p-aminobenzoylhydrazone (3), p-hydroxybenzaldehyde p-amino- benzoylhydrazone ( 4 ), naphthaldehyde p-aminobenzoylhydrazone (5) and p-methoxybenzaldehyde paminobenzoylhydrazone (6) to resorcinol and through diazo-coupling reactions. Mixed -ligand dinuclear complexes with general stoichiometry [Cu2L(amino antipy.)2]Cl2 (11, 12, 13 and 14) was synthesed from azo ligands and bidentate ligand (4-aminoantipyrine) with copper. The structures of both azo dyes and their complexes were identified by elemental analyses (C.H.N), FTIR, 1 H-NMR,
Time course of hepatitis A viremia and viral load in the blood of human hepatitis A patients

The hepatitis A virus (HAV) is the most common etiological cause of acute hepatitis infections in humans in industrialized countries. Investigations into the viral load during HAV viremia, however, are rare. Therefore, correlation studies between viral load, biochemical, and specific serological markers have been undertaken. The group of sera comprised a series of multiple consecutive blood samples drawn from 11 patients at different times after onset of the disease. During the period up to 70 days after the onset of icterus, the individual range was at 1×103 to 3×104 HAV genome equivalents/ml. From day 75 until 120 after onset of the disease, the levels traced were at 103. In one case, it was possible to trace 1.25×104 genome equivalents/ml up to 180 days after onset of icterus and in two cases even up to 408 and 490 days viral load levels of 5×103 and 4×104 were detected, respectively. The same sera were used to measure IgM class antibodies to hepatitis A virus and the total anti‐HAV. The results demonstrate that a direct correlation to peak levels of viral load exists with peak serum transaminase levels, but neither with peak anti‐HAV IgM levels nor with total anti‐HAV. Decreasing amounts of anti‐HAV IgM tend to occur with decreasing amounts of HAV genome equivalents; and, vice versa, increasing amounts of total anti‐HAV are accompanied by decreasing amounts of HAV genome equivalents. The longest duration of viremia was found in patients infected with HAV genotype IA. J. Med. Virol. 72:10–16, 2004. © 2004 Wiley‐Liss, Inc.
Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila

The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ∼12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO2 fixation.
Preparation and characterisation of andrographolide niosomes and its anti-hepatocellular carcinoma activity

Abstract Objective: In this study, a novel andrographolide (AG) preparation formulation, niosomes, was prepared to improve the bioavailability and tissue distribution of AG. Methods: The niosomal formulation of AG was prepared by film hydration/sonication method and tissue distribution was measured by liquid chromatography-mass spectrometry (LC-MS) method in mice, and anti-hepatocellular carcinoma (anti-HCC) activity was examined by MTT method in HepG2. Results: Entrapment efficiency, drug-loading ratio and average particle size of AG niosomes were 72.36%, 5.90% and 206 nm, respectively. The tissue distribution in mice demonstrated that the AG niosomes were absorbed in liver much more than the free AG. Furthermore, the anti-HCC activity in HepG2 cells showed that there was no significant difference between free AG and AG niosomes. Conclusion: The present results suggest that AG niosomes may have a significant potential of liver targeting, which is valuable in chemotherapy of HCC.
Design of microstrip antenna for modern wireless communication

In this paper, the design of microstrip antenna for modern wireless communication is presented. The proposed antenna has a fairly good performance of impedance bandwidth at 2.40 GHz. The antenna is designed on size of 46 × 37 × 1.5 mm3 and fed with 50 Ω microstrip line. Satisfactory performance was obtained by using TLC 30 (TACONIC)substrate, allowing low cost production, Tightly controlled Dk, low Df, excellent dimentional stability and high flexural strength. It covers the frequency bands 1.37 and 2.40 GHz which includes GPS and WLAN. The proposed microstrip antenna for wireless communication, consists of two major combined radiating elements: a rectangular and triangular patch element. These two elements are interconnected by step and patch B1 elements. It is found that the effect on frequency band depends on the length of the partial ground, position of steps, length of the rectangle and height of triangle in order to obtain a better S11 value. The design antenna able to obtain power gain of 2.7 dBi. Results are obtained using CST Microwave studio.