Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of a eukaryotic‐like protein kinase gene in Archaebacteria

Primary sequence patterns based on known conserved sites in eukaryotic protein kinases were used to search for eukaryotic‐like protein kinase sequences in a six‐frame translation of the bacterial subsection of GenBank. This search identified a previously unrecognized eukaryotic‐like protein kinase gene in three related methanogenic archaebacteria, Methanococcus vannielii, M. voltae, and M. thermolithotrophicus. The proposed coding sequences are located in orthologous open reading frames (ORFs): ORF547, ORF294, and ORF114, respectively. The C‐terminus of the ORFs contains 9 of the 11 subdomains characteristically conserved within the eukaryotic protein kinase catalytic domain. The N‐terminus of the ORFs is similar to a putative glycoprotease in Pasteurella haemolytica and its homologue in Escherichia coli, the orfX gene. This is the first report of a eukaryotic‐like protein kinase sequence observed in Archaebacteria.
Impact of transmit antenna beamwidth for fixed relay links using ray-tracing and Winner II channel models

The BuNGee (Beyond Next Generation Mobile Broadband) project aims to improve the overall infrastructure capacity density of mobile networks to an ambitious goal of 1Gbps/km2 in economical cellular deployment [1]. Its innovative architecture combines a number of technological paradigms, such as self-backhauling, joint design of access and backhaul, etc. It relies on a Hub Base Station (HBS) connected to the operator back-haul, distributed Access Base Stations (ABSs) connected to the HBS by a self-backhaul link on one side and to the Mobile Subscribers (MSs) on the other side. In this communication, we focus on the specificities of the propagation channel between HBS and ABS, as it is largely different from the channel between ABS and MS. Whilst a large amount of viable channel models, e.g. 3GPP, COST 273 and WINNER II, are available, they require some significant modifications to reflect the peculiarities of BuNGee architecture, in particular the transmit antenna specifications. Hence, pathloss models for the HBS-to-ABS link are investigated by a ray-tracing tool and simultaneously compared with the potential of WINNER II channel models. Furthermore, the impact of the transmitter's narrow beamwidth is investigated and analyzed.
Preliminary radiochemical and biological studies on the liposome encapsulated platinum-[125I]iodohistamine complex.

BACKGROUND The platinum-iodohistamine complex with in vitro cytostatic activity toward colon and mammary cancer cells has been synthesised recently in our laboratory. The pharmacokinetics of radioactive complex analogues, labelled with I-131 and I-125, has been examined in murine model of spontaneous mammary adenocarcinoma. The present work is devoted to the examination of the potential use of liposomes as a carrier system for the radioactive platinum-[*I]iodohistamine complex in vivo. MATERIAL AND METHODS Encapsulations of the Pt- [(125)I]iodohistamine were studied using a different molar ratio of the complex and liposomes with positive surface charge, as well as various incubation procedures. Biodistribution of the initial and the liposomal form of the complex were studied in C3H tumour-bearing mice with spontaneously developed and transplantable (16C) mammary adenocarcinoma. RESULTS Comparative biodistribution studies in C3H/16C mice and in mice with spontaneously developed mammary tumour have shown that in the former model pharmacokinetics of the Pt-[125I]iodohistamine complex is more predictable and more similar to that observed for cisplatin. Therefore, the transplantable tumour model is more advantageous for the complex and its liposomal form evaluation. In C3H/16C mice, significant differences in the biodistribution between the radioactive platinum complex and its liposomal form were observed. The concentration of the activity in blood after 2 h p.i. v. was two times lower for the encapsulated complex, and the uptake of the radioactivity by liver, spleen, and lungs was twice as high as that obtained for the free Pt-[(125)I]iodohistamine preparation. The radioactivity in tumour was almost constant for liposomal platinum complex (ca. 2% ID/g), although it was two times lower compared to the initial platinum complex. CONCLUSIONS The results of the present study indicate that platinum-[*I]iodohistamine can be efficiently incorporated into cationic liposomes (c. 40%). However, the uptake of the encapsulated complex by the liver and spleen macrophages demands further modification of the lipid membrane.
Activated cell survival cascade protects cardiomyocytes from cell death in Tako‐Tsubo cardiomyopathy

Tako‐Tsubo cardiomyopathy (TTC) is characterized by rapid regeneration of contractile dysfunction. From recent studies it is known that excessive catecholamine levels due to emotional or physical stress might play a central role. After sympathetic activation, the PIK3/AKT pathway is a key regulator of many cellular responses, including cytoprotective effects. Thus, the purpose of this study was to investigate whether the PIK3/AKT pathway plays a pivotal role in TTC.
SUBARACHNOID CLOT VOLUME CORRELATES WITH AGE, NEUROLOGICAL GRADE, AND BLOOD PRESSURE

OBJECTIVECerebral vasospasm after aneurysmal subarachnoid hemorrhage (SAH) is associated with the volume and location of subarachnoid blood clots. Factors that influence the volume of SAH have seldom been studied. METHODSTwo independent sets of data were analyzed. Data from 3028 patients with SAH enrolled in four clinical trials of the drug tirilazad were analyzed in addition to data from 74 patients with SAH who underwent digital volumetric analysis of admission computed tomographic scans to determine the subarachnoid clot volume. In the smaller sample of 74 patients, aneurysm width, length, neck size, aspect ratio, and volume were measured on diagnostic cerebral angiograms. Statistical inference bearing on the question of what factors are associated with clot volume was derived by univariate methods, including analysis of variance, χ2 and t tests, and polytomous logistic regression. RESULTSOf 22 clinical parameters examined by univariate analysis of the tirilazad dataset, age, World Federation of Neurological Surgeons (WFNS) clinical grade, time from SAH to admission, history of hypertension or diabetes mellitus, aneurysm location, and admission diastolic and systolic blood pressure were correlated with the subarachnoid clot volume (P < 0.05). Polytomous logistic regression found that only age, WFNS grade, time to admission, admission systolic blood pressure, and history of hypertension were higher in patients with larger subarachnoid clots (P < 0.05). Analysis of 74 patients with quantitative subarachnoid clot volumes also found that age and WFNS grade were higher in patients with larger subarachnoid clots (P < 0.05). No aneurysm location or measurement of aneurysm size showed a statistically significant relationship to clot volume in either dataset. CONCLUSIONSAH volume is correlated with clinical characteristics, including age, history of hypertension, admission systolic blood pressure, and WFNS grade. Anatomic aneurysm characteristics such as size and location do not reliably predict clot volume.
Effect of myogenic and adipogenic differentiation on expression of colony-stimulating factor genes

The influence of cellular differentiation on colony-stimulating factor gene expression was examined in myogenically and adipogenically determined cell lines derived from 5-azacytidine-treated C3H10T1/2 C18 (10T1/2) mouse embryo fibroblasts. These studies demonstrate that colony-stimulating factor gene expression can be modulated by myogenic and adipogenic determination and terminal differentiation.
The PD-1 pathway as a therapeutic target to overcome immune escape mechanisms in cancer

Introduction: Immunotherapy is emerging as a powerful approach in cancer treatment. Preclinical data predicted the antineoplastic effects seen in clinical trials of programmed death-1 (PD-1) pathway inhibitors, as well as their observed toxicities. The results of early clinical trials are extraordinarily promising in several cancer types and have shaped the direction of ongoing and future studies. Areas covered: This review describes the biological rationale for targeting the PD-1 pathway with monoclonal antibodies for the treatment of cancer as a context for examining the results of early clinical trials. It also surveys the landscape of ongoing clinical trials and discusses their anticipated strengths and limitations. Expert opinion: PD-1 pathway inhibition represents a new frontier in cancer immunotherapy, which shows clear evidence of activity in various tumor types including NSCLC and melanoma. Ongoing and upcoming trials will examine optimal combinations of these agents, which should further define their role across tumor types. Current limitations include the absence of a reliable companion diagnostic to predict likely responders, as well as lack of data in early-stage cancer when treatment has the potential to increase cure rates.
Vitamin B6 suppresses serine protease inhibitor 3 expression in the colon of rats and in TNF-α-stimulated HT-29 cells.

SCOPE Previous reports in the areas of animal studies and, recently epidemiology, have linked anti-tumorigenic and anti-inflammatory effects to dietary vitamin B6. This study investigated the molecular mechanism of these effects of vitamin B6. METHODS AND RESULTS DNA microarray analysis was used to obtain information on changes in colon gene expression from vitamin B6 (pyridoxine) repletion in vitamin B6-deficient rats. Pyridoxine supplementation down-regulated the inflammatory molecule, serine protease inhibitor clade A member 3 (SPI-3) mRNA expression in the colon. This study also showed that tumor necrosis factor α (TNF-α) induced SPI-3 mRNA expression in HT-29 human colon cancer cells, and vitamin B6 (pyridoxal hydrochloride) pretreatment of HT-29 cells inhibited TNF -induced mRNA expression of SPI-3. Vitamin B6 inhibited TNF-α-induced NF-κB activation via suppression of IκBα degradation in HT-29 cells. HT-29 cells stably expressing epitope-tagged ubiquitin were generated and vitamin B6 pretreatment was shown to inhibit ubiquitination of the IkB protein in response to TNF-α-i. CONCLUSION Vitamin B6 suppressed SPI-3 expression in the colon of rats and in TNF-α-stimulated HT-29 cells. Further, this study showed a possible role of vitamin B6 in the regulation of protein ubiquitination.
Births: final data for 2011.

OBJECTIVES This report presents 2011 data on U.S. births according to a wide variety of characteristics. Data are presented for maternal characteristics, including age, live-birth order, race and Hispanic origin, marital status, attendant at birth, method of delivery, and infant characteristics (e.g., period of gestation, birthweight, and plurality). Birth and fertility rates are presented by age, live-birth order, race and Hispanic origin, and marital status. Selected data by mother's state of residence and birth rates by age and race of father also are shown. Trends in fertility patterns and maternal and infant characteristics are described and interpreted. METHODS Descriptive tabulations of data reported on the birth certificates of the 3.95 million births that occurred in 2011 are presented. Denominators for population-based rates are postcensal estimates derived from the U.S. 2010 census. Birth and fertility rates for 2001-2009 are based on revised intercensal population estimates. Denominators for 2011 and 2010 rates for the specific Hispanic groups are derived from the American Community Survey; denominators for earlier years are derived from the Current Population Survey. RESULTS The number of births declined 1% in 2011 to 3,953,590. The general fertility rate also declined 1%, to 63.2 per 1,000 women aged 15-44. The teen birth rate fell 8%, to 31.3 per 1,000 women. Birth rates declined for women in their 20s, were unchanged for women aged 30-34, and rose for women aged 35-44. The total fertility rate (estimated number of births over a woman's lifetime) declined 2% to 1,894 per 1,000 women. The number and rate of births to unmarried women declined; the percentage of births to unmarried women was essentially stable at 40.7%. The cesarean delivery rate was unchanged from 2010 at 32.8%. The preterm birth rate declined for the fifth straight year to 11.73%; the low birthweight rate declined slightly to 8.10%. The twin birth rate was not significantly changed at 33.2 per 1,000 births; the rate of triplet and higher-order multiple births also was essentially stable at 137.0 per 100,000.
Dynamic Cellular Manufacturing under Multiperiod Planning Horizons

In this paper cellular manufacturing is discussed under conditions of changing product demand. Traditional cell formation procedures ignore any changes in demand over time from product redesign and other factors. However given that in today's business environment, product life cycles are short, a framework is proposed that creates a multi-period cellular layout plan including cell redesign where appropriate. The framework is illustrated using a two stage procedure based on thegeneralized machine assignment problem and dynamic programming. This framework is conceptually compared to virtual cell manufacturing, which is useful when there is uncertainty in demand rather than anticipated changes in demand. Thus this paper should be useful to both researchers and Practitioners who deal with demand changes in cellular manufacturing.
ANTIQUITY IMPACT OF AIR POLLUTION AT GADARA, JORDAN

Several recent studies have pointed out that the northern parts of Jordan are exposed to high levels of ground ozone. North Jordan is a wealthy reservoir of ancient heritage including six out of the ten Decapolis Cities. It is alleged that air pollution-via acid depositionhas led to the deterioration and erosion of buildings, structures, statues, and monuments. Therefore, this research is conducted to assess air quality at Gadara, the capital of the ancient Decapolis and to come up with mitigation measures that have to be adopted in order to save the ancient heritage from further deterioration. Measurements revealed that most criteria air pollutants including sulfur dioxide, nitrogen dioxide, and carbon monoxide attain corresponding national ambient air quality standards. However, ground ozone, which is believed to expedite the deterioration rate of rocks in the presence of acids as they act as a catalytic agent, exceeds the limit recommended by the World Health Organization of 50 ppb.
Detection of Feedback Reputation Fraud in Taobao Using Social Network Theory

As the number of shoppers in C2C sites is rapidly growing in China, online customers may suffer from online fraud. The feedback reputation system has been used to prevent abusing behavior and secure transaction. However, large numbers of fraudsters manipulated their feedback scores by engaging in a number of legitimate sales. Furthermore, there exist agents of "Credit speculation", who boost the fraudsters' scores professionally in China. Since fraud behaviors are subtle and complex. it makes the fraudulent behavior more difficult to discover. In this study, we seek for extracting characteristic features in fraudsters' behaviors. We present an approach for analyzing online crediting behaviors, which utilizes the social network analysis (SNA) technique to detect the relationship of potential fraudsters. Some cases of real data from Taobao demonstrate our analyses.
Detection of enterotoxigenic Escherichia coli by colony hybridization with biotinylated enterotoxin probes

By using biotinylated enterotoxin DNA probes, a method to detect enterotoxigenic Escherichia coli by colony hybridization was developed. The treatment of colonies on nitrocellulose membrane filters with proteinase K and Triton X-100 was essential for obtaining the specific hybridization. A total of 200 E. coli strains isolated from travelers with diarrhea were tested for colony hybridization by using a probe encoding heat-labile toxin (LT) type h. All strains (86 of 86) that produced LT, but none of the non-LT producers, hybridized with 32P-labeled and biotinylated LT type h probes. A total of 36 strains chosen randomly from the 200 isolates were tested for colony hybridization by using heat-stable enterotoxin (ST) probes. All but two strains that hybridized with the 32P-labeled ST type Ia probe also hybridized with the biotinylated ST type Ia probe. All strains that hybridized with the 32P-labeled ST type Ib probe also hybridized with the biotinylated ST type Ib probe. Thus, almost all E. coli strains tested were judged to be the same by colony hybridization with biotinylated or 32P-labeled enterotoxin probes. These results demonstrate that the biotinylated enterotoxin probes are useful in the diagnosis of enterotoxigenic E. coli strains by colony hybridization.
Fibrinogen and fibrin induce synthesis of proinflammatory cytokines from isolated peripheral blood mononuclear cells

Summary Fibrinogen in plasma includes three main fractions; high-molecular- weight (HMW) -fibrinogen, low-molecular-weight (LMW) -fibrinogen, and very-low-molecular-weight (LMW`) -fibrinogen. During acute-phase conditions, plasma fibrinogen levels and the HMW-/LMW-fibrinogen ratio increase rapidly due to increased synthesis of HMW-fibrinogen. The consequences of elevated plasma fibrinogen levels and local deposition of fibrin in inflammatory tissues observed during acute-phase conditions are not clear.We wanted to investigate proinflammatory effects of fibrinogen and fibrin on peripheral blood mononuclear cells (PBMC) as reflected by altered mRNA expression and synthesis of the proinflammatory cytokines IL-6,TNF- α and IL-1 β, and to explore the significance of altered HMW-/LMW-fibrinogen ratio. PBMC were isolated from whole blood using Lymphoprep® . HMW-fibrinogen was separated from unfractioned fibrinogen by ammonium sulphate precipitation. Cells were incubated with unfractioned fibrinogen, HMW-fibrinogen or fibrin. Cytokine levels in cell lysates were determined using ELISA assays. Real-time PCR was used for mRNA quantification. We found that fibrinogen significantly increased mRNA levels, and induced synthesis of the proinflammatory cytokines IL-6 and TNF- α in PBMC in a dose dependent manner. Median (25, 75 percentile) IL-6 and TNF- α concentrations were 12 (5, 40) pg/ ml and 16 (0,61) pg/ml,respectively.Median mRNA quantity was increased 12.3– (6.6, 48.6) and 1.7– (1.5, 6.5) fold for IL-6 and TNF- α compared to controls.The stimulatory effect of unfractioned fibrinogen was not significantly different from HMW-fibrinogen. Fibrinogen and fibrin were equally effective in promoting cytokine synthesis from PBMC.The results support that fibrin and fibrinogen may actively modulate the inflammatory process by inducing synthesis of proinflammatory cytokines from PBMC.
Application of Trichoderma reesei Cellulase and Xylanase Promoters through Homologous Recombination for Enhanced Production of Extracellular β-Glucosidase I

One of the limiting factors for the application of Trichoderma reesei to degrade cellulosic biomass is its low β-glucosidase activity, required to convert cellobiose to glucose. The egl3 and the xyn3 promoters were used to express β-glucosidase 1 gene bgl1 through homologous recombination to improve the cellulose degradation ability of T. reesei. The recombinant strains expressing β-glucosidase 1 (BGLI) under the control of either the egl3 or the xyn3 promoter had 4.0 and 7.5 fold higher β-glucosidase activity than the native strain, which compares well to the finding that in wild-type T. reesei PC-3-7, the levels of egl3 and xyn3 mRNA expression were 6.0 and 12 fold higher respectively than that of bgl1. Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis of proteins secreted by the recombinant strains demonstrated that BGLI was overproduced. The increase in the transcription of bgl1 and the concomitant elevated level of BGLI in these recombinant strains were sufficient to degrade the cellobiose and cellotriose formed during the degradation of pretreated cedar powder.
Blood Tumor Prediction Using Data Mining Techniques

Healthcare systems generate a huge data collected from medical tests. Data mining is the computing process of discovering patterns in large data sets such as medical examinations. Blood diseases are not an exception; there are many test data can be collected from their patients. In this paper, we applied data mining techniques to discover the relations between blood test characteristics and blood tumor in order to predict the disease in an early stage, which can be used to enhance the curing ability. We conducted experiments in our blood test dataset using three different data mining techniques which are association rules, rule induction and deep learning. The goal of our experiments is to generate models that can distinguish patients with normal blood disease from patients who have blood tumor. We evaluated our results using different metrics applied on real data collected from Gaza European hospital in Palestine. The final results showed that association rules could give us the relationship between blood test characteristics and blood tumor. Also, it demonstrated that deep learning classifiers has the best ability to predict tumor types of blood diseases with an accuracy of 79.45%. Also, rule induction gave us an explanation of rules that describes both tumor in blood and normal hematology.
3DMIRACLES: 3D model retrieval and visualization engine

The research on 3D model retrieval is a new hot point and meanwhile a difficult problem in the area of content based retrieval. In this paper, a novel 3D model retrieval and visualization engine, 3DMIRACLES (3D Multimedia Information RetrievAl, CLassification and Exploration System), has been developed, which integrates effective algorithms and techniques for both shape-based retrieval computation of 3D models and real-time visualization of the retrieval results in realistic 3D interactive mode. The system architecture of 3DMIRACLES has been proposed. For retrieval computation, new algorithms have been developed and implemented for 3D shape feature extraction and similarity matching, which mainly include slice based method, Delta functions method, thickness histogram method and directional form, etc. For interactive visualization, a novel 3D viewer has been developed based on the foundational programming library of 3D drawing developed by our group, which implements hybrid rendering method to make much simplification and shortcut processing of 3D rendering computation for achieving high speed and efficient real-time visualization of large scale 3D databases. Evaluation experiments show that 3DMIRACLES is successful and effective in 3D shape retrieval and visualization for large scale 3D model databases, and the research achievements may be applied to real application system.
EVIDENCED-BASED CLINICAL MEDICINE Colposcopy : An Evidence-Based Update

Colposcopy is a diagnostic procedure, most commonly used in the diagnosis of cervical intraepithelial neoplasia and lower genital tract carcinoma. In this article, evidence-based management strategies are updated with discussion of the 2001 American Society for Colposcopy and Cervical Pathology Consensus Guidelines. Practice management issues include methods to improve cervical cancer screening rates, coding and billing, and telemedicine. Textbooks, CD-ROMs, and courses are listed for new learners and experienced providers who want to update and sharpen their skills. (J Am Board Fam Pract 2005;18: 383–92.)
Operational Characteristics of a 30kWW-band Gyrotron Developed at KERI

We report operational characteristics of a 30kW W-band gyrotron developed at KERI through holistic integration of home-made DC power supplies and a superconductor magnet. Auxiliary power supply combined to the main power supply providing the emission current of 2A takes part of the accelerating potential of 50kV up to 18kV and increases the overall efficiency of the gyrotron system up to 48%.