Miracle-dz/UniFAR-FTUs huggingface dataset:
Índices de crecimiento de plántulas de lulo (Solanum quitoense [Lamarck.]) en sustratos orgánicos

El lulo (Solanum quitoense [Lamarck.]) es una especie con alto potencial de comercializacion a nivel global dado el valor nutritivo de sus frutos. En Mexico, la investigacion sobre esta especie es escasa y uno de los primeros retos es la obtencion de plantulas vigorosas que garanticen rendimiento y buena calidad de frutos. En esta investigacion se estimaron indices de crecimiento de plantulas de lulo provenientes de semillas germinadas y crecidas en tres sustratos organicos constituidos por turba y composta en diferentes relaciones (60/40, 40/60 y 20/80, v/v) en invernadero. El experimento tuvo una distribucion completamente al azar con cuatro repeticiones, y la unidad experimental contenia 32 plantulas. Sesenta dias despues de la siembra se tomaron datos directos de crecimiento para la estimacion de los indices siguientes: razon de area foliar (RAF), area foliar especifica (AFE), proporcion de hoja (PH) y proporcion de raiz (PR). Los datos obtenidos fueron analizados estadisticamente, realizando un analisis de varianza y una prueba de comparacion de medias. Los resultados indican que la composta en mayor proporcion en el sustrato reduce la RAF, el AFE y PH; por el contrario, incrementa la PR. La adicion de composta al sustrato en proporcion 80%, afecto negativamente los indices de crecimiento de las plantulas de lulo debido al incremento en la conductividad electrica, y de Na+, Cl- y HCO3- solubles, en el sustrato. Se concluye que la composta puede ser usada con resultados satisfactorios, en la produccion de plantulas de lulo, en combinacion con turba, en proporciones iguales o menores a 60%.
Speeding in urban South East Asia: results from a multi-site observational study

Speed is an important risk factor for road traf c crashes. We studied the prevalence of speeding in three major cities in Southeast Asia (Bandung, Bangkok and Ho Chi Minh City-HCMC), and factors associated with it. The study was conducted between July 2015-September 2016 using a standardized protocol and data collection tool. Observation locations were selected through systematic sampling. Speed was measured using a microwave radar gun on weekdays and weekends, and 5 times during each day. Descriptive and logistic regression analysis was done separately for each city. 623,744 vehicles were observed (Bangkok:61.8%; Bandung:36.0% and HCMC:2.2%). 21.8% vehicles were found to be speeding across the three cities. The prevalence of speeding was 7.8% in Bandung, 30.7% in Bangkok, and 1.9% in HCMC. When adjusted for other variables, compared to motorcycles, SUVs were more likely to be speeding in Bandung (aOR:1.97); large trucks (aOR:7.69) in Bangkok; and light trucks in HCMC (aOR:2.39). In Bandung, speeding was mostly observed in the peri-urban parts of the city (94.5%). Speed was likely to be highest during non-peak hours of the day in HCMC (aOR:3.08). High prevalence of speeding was observed in the three cities, making this an important risk factor for road safety in urban Southeast Asia. FINDINGS, especially with regards to variations by vehicle type, times of day, days of week, and types of roads would be useful for city governments and traf c police to better plan strategies to improve road safety in these cities. Key words Speeding, Asia, developing countries, multi-site analysis Language: en
Chicken genome mapping - Constructing part of a road map for mining this bird's DNA

The aim of the research presented in this thesis was to aid in the international chicken genome mapping effort. To this purpose, a significant contribution was made to the construction of the chicken whole-genome BAC-based physical map (presented in Chapter A). An important aspect of this construction was the integration of the genetic and physical maps (see Chapters 2 and 3) in order to anchor clone-contigs to a chromosomal location.In addition to the construction of the physical map, two different SNP resources were analyzed. First, it is shown in Chapter 5 that cione-based sequencing is a valid SNP discovery strategy. Second, an anaLysis of public SNP repositories was presented, focussing on intra-genic SNP discovery in human (Chapter 6) which pinpointed some issues related to mining public SNP databases. In Chapter 7, the extent of linkage disequiiibrium -~ reflecting the history of recombinations - was assessed in two regions of the chicken genome. This information is useful for setting up linkage and association studies. Finally, a software tool was described (Chapter 8) that was implemented to automate the analysis of sequencing data, which is especially important in major mapping experiments as described in this thesis.
Whither Happiness? : When, How, and Why Might Positive Activities Undermine Well-Being

Across the globe, most people desire happiness (Diener, 2000; Diener, Suh, Smith, & Shao, 1995), and this desire transcends differences in age, culture, geographical location, political beliefs, religion, and life experiences. The quest for the secret of how to increase and sustain happiness has preoccupied men and women for millennia, from the philosophers of ancient Athens to present-day scientists and scholars (Kesebir & Diener, 2008; McMahon, 2008). Although conceptualizations of happiness may shift across generations and cultures, the goal of attaining it remains ubiquitous (McMahon, 2008; Oishi, Graham, Kesebir, & Galinha, 2013). Notably, happiness is desirable not just because it is pleasurable, but because it grants numerous benefits to both the individual and those around him or her (see also Fredrickson, this volume). Happiness (or well-being)—which researchers define as the experience of frequent positive emotions relative to negative emotions, coupled with high life satisfaction—predicts, correlates, and begets success across multiple life domains, including work, relationships, and physical health (Lyubomirsky, King, & Diener, 2005; see also von Hippel & Gonsalkorale; and Sheldon, this volume). Relative to their less happy counterparts, happy people have stronger interpersonal relationships, higher incomes, and superior physical and mental health; they receive more favorable job performance reviews, are more likely to get married, and live longer on average (e.g., Boehm & Kubzansky, 2012; Chida & Steptoe, 2008; Lyubomirsky, King, et al., 2005; Simpson, Farrell, Huelsnitz, & Eller, this volume). Positive emotions have also been shown to prompt greater creativity and prosocial behavior (see Lyubomirsky, King, et al., 2005, for a review). Happiness, therefore, does not only feel good—it is good. It pays to be happy not just for the individual, but for the individual’s social network and his or her community at large. In this chapter, we will use the terms happiness and well-being interchangeably.
Functional analysis of the JIL-1 histone H3 kinase in Drosophila

Epigenetic regulations play a crucial role in control of gene expression and development. Histone modification is one of the epigenetic mechanisms that contribute to regulation of chromatin structure and gene expression. In Drosophila melanogaster, JIL-1, the predominant interphase histone H3S10 kinase, localizes specifically to euchromatic interband regions of polytene chromosomes, and is upregulated two-fold on the male X chromosome. Genetic interaction assays with JIL-1 hypomorphic and null allelic combinations demonstrated that JIL-1 can counterbalance the gene-silencing effect of the three major heterochromatin components Su(var)3-9, Su(var)3-7, and HP1a on positioneffect variegation. Our data suggested that the epigenetic H3S10ph mark functions to counteract heterochromatic spreading and gene silencing in Drosophila . JIL-1 can be divided into four main domains including a NH2-terminal domain (NTD), the first kinase domain (KDI), the second kinase domain (KDII), and a COOH-terminal domain (CTD). Transgenic analysis demonstrated that the CTD of JIL-1 is necessary and sufficient for correct chromosome targeting to autosomes, but that both COOHand NH2-terminal sequences were necessary for upregulation on the male X chromosome. Another construct ∆CTD that lacks the CTD domain but has histone H3S10 kinase activity can be localized to chromatin by the NTD and was able to rescue autosomes as well as partially rescue male X polytene chromosome morphology. Furthermore, to study the role of histone H3S10 phosphorylation in transcription we examined the distribution of JIL-1 and histone H3S10 phosphorylation under both heat shock and non-heat shock conditions. There was no redistribution or upregulation of JIL-1 or histone H3S10 phosphorylation found at transcriptionally active puffs after heat shock treatments. Also in JIL-1 null mutant backgrounds, heat shock-induced puffs were strongly labeled by the antibody to the elongating form of RNA polymerase II (Pol IIo), indicating
Potentiel probiotique des bactéries lactiques de l'écosystème mammaire bovin contre les mammites à Staphylococcus aureus

Staphylococcus aureus est un des pathogenes majeurs implique dans les mammites chez les ruminants. Il engendre des tableaux cliniques tres variable, allant des mammites subcliniques aux mammites gangreneuses, mais les mecanismes de cette variabilite restent encore mal connu. La volonte de reduire l'utilisation d'antibiotiques en medecine veterinaire ainsi que leurs faibles efficacites contre les mammites staphylococciques soulignent la necessite de developper de nouvelles strategies alternatives. L'une des particularites des infections intramammaires a S. aureus est leur pouvoir chronique et persistent dont l'une des principales causes est la capacite de S. aureus a adherer et internaliser dans les tissus de l'Hote. L'un des concepts ayant fait ses preuves chez l'Homme et l'animal se base sur le concept de la lutte biologique en utilisant les proprietes inhibitrices des bacteries dites « probiotique » d'un ecosysteme pour reduire, prevenir ou traiter les infections. Cette etude vise a mieux caracteriser le microbiote mammaire et notamment la composante lactique qui par analogie avec les autres ecosystemes jouent un role positif sur la sante de l'Hote et de determiner son potentiel a moduler l'adhesion et l'internalisation de S. aureus. L'ensemble de ce travail a ete realise avec deux souches de S. aureus tres differentes au niveau des tableaux cliniques engendres. La souche RF122, tres virulente et la souche Newbould 305, induisant des infections chroniques. Deux messages clairs ressortent des analyses independantes du pathogene et du microbiote : d'une part, l'edute comparative in silico et in vitro revele que l'invasion cellulaire semble etre majeur pour la chronicite de la souche Newbould 305 et d'autre part que l'ecosysteme de la glande mammaire presente une composante lactique ayant des capacites probiotiques interessantes. Dans notr! e modele d'interaction tripartite, trois acteurs ont ete retenus, S. aureus, Lactobacillus casei et les cellules epitheliales mammaires MAC-T. Dans ce systeme, L. casei affecte l'adhesion et surtout l'internalisation de S. aureus sans modifier la viabilite et la morphologie des cellules en culture. Les mecanismes identifies dans l'inhibition semblent dependant d'un contact direct avec des L. casei viables et permettent de poursuivre la caracterisation des bases moleculaires de ce phenomenes impliquant une modulation des genes de virulence et cellulaire lors d'interaction avec une flore competitrice. Ces resultats ouvrent de nouvelles perspectives dans l'application de bacteries lactiques en tant que probiotique mammaire.
Response of understory vegetation to ponderosa pine thinning in eastern Washington.

ranges in the central Rocky Mounfeed. II. A rapid method for the taini. Iowa State Coll. J. ‘Sci. 34: 73 l-748. determination Gf fiber and lignin. 1. Ass. Offic. Agr. Chem. 46:829Longmont Seed Co. TILLEY, J. M. , AND R. A. TERRY. 1963. Ys35. We Buy & Sell Western Range Grass A two-stage technique for the in WASSER, C. H. , AND J. L. RETZER. & Legume Seeds vitro digestion of forage crops. J. 1966. Ecology and utility of the CONFRONT US with your RANGE British Grassland Sot. 18: 104-l 11. central Rocky Mountain alpine zone. PROBLEMS: phone: 303 + 776-1320 VAN SOEST, P. J. 1963. Use of deProc. 9th Int. Grassland Congr. , p. LONGMONT, COLORADO 80501 tergents in the analysis of fibrous 357-361.
Gene Therapy of Liver Disease with Lentiviral Vectors Preclinical Studies in Models of Crigler-Najjar Disease and Hepatitis C

textabstractThe main theme of this thesis is the application of lentiviral vectors for the treatment of congenital and acquired liver disease. Gene therapy represents a relatively new and promising therapeutic tool with possible applications in a broad spectrum of medical disciplines. The underlying principle of gene therapy is based on the introduction of genetic material into living cells to achieve a therapeutic biological effect. Consequently, gene therapy focuses on treating the cause of disease rather than the symptoms. The first part of this introduction will review the current options and developments in the field of liver directed gene therapy (1.1). Next we will review the most widely used viral vector systems (1.2). HIV-1 derived lentiviral vectors are among the most efficient gene therapy vectors that are currently available. The capacity of lentiviral vectors to stably integrate into the target cell genome of non-dividing cells is one of the main distinguishing features of this vector system. Clinical trials that involve the ex vivo transduction and retransplantation of autologous CD4+ T-cells or hematopoietic stem cells have been initiated [1-3]. In preclinical experiments, lentiviral vectors efficiently transduce differentiated liver parenchymal cells in vivo [4, 5]. Other major targets, such as the central nervous system and the airways are also under intense investigation [6, 7]. In the third part of this introduction, we will introduce this relatively new vector system that plays a central role in our studies (1.3). To learn how a dangerous pathogen such as HIV-1 was transformed into an efficient and safe gene transfer vehicle, the HIV-1 genome and virion composition and the viral lifecycle will be reviewed. Furthermore, we will discuss the lentiviral vector production procedure and transduction process. Finally, the scope of the thesis is outlined by introducing each chapter (1.4).
Feasibility of fuzzy-logic based systems on IM torque estimation

This paper presents a new method for online torque estimation of an induction motor (IM). It uses a fuzzy logic-based system with five inputs: the measured stator winding temperature, the measured stationary d and q components of the stator current, and the estimated stationary d and q components of the rotor flux. The estimator has been trained using a simulator of an IM, and then implemented on a digital signal processor. Experimental results obtained with a rotor-flux-oriented vector-controlled 10 HP drive show good agreement between estimated and real torque.
Do the habitats, mouth morphology and diets of the mullids Upeneichthys stotti and U. lineatusin coastal waters of south-western Australia differ?

Two benthic carnivorous goatfish (Mullidae), Upeneichthys stotti (max total length, LT= 179 mm) and U. lineatus (max LT= 257 mm), were trawled from the inner continental shelf waters of Western Australia. U. stotti was found almost exclusively offshore at 20–35 m depth, while U. lineatus was most abundant inshore at 5–15 m depth. Smaller individuals of both species ate small, thin-shelled mysids, tanaids and amphipods, while larger fish ate large, hard-bodied isopods, carid decapods and brachyuran crabs. Classification and ordination of the mean volumetric percentage contributions of the prey (dietary samples) of both species from all sites and in each season did not lead to a clear separation between these two species. However, ordination demonstrated that the dietary samples of the two species were distinct when the two species were found together, and when fish of sequential 20-mm length intervals were used. The interspecific size-class differences were consistent with the results obtained using Schoener's overlap index, for which values >0·6 were recorded for only two of the 45 possible interspecific pairwise comparisons. Since these interspecific differences occurred despite virtually identical mouth sizes and morphologies, the two species must feed in a slightly different manner and/or in different microhabitats. While most comparable-sized fish ate tanaids and amphipods, U. stotti ate more relatively mobile epibenthic mysids, cumaceans and carid decapods, whereas U. lineatus ate more larger and slower-moving burrowing bivalves, onuphid polychaetes and brachyuran crabs. The partial partitioning of habitats, when combined with differences in the diet, would help facilitate the co-existence of these two mullids when they are abundant.
Cell culture technology for pharmaceutical and cell-based therapies

Preface. Cell Culture Technology: An Overview. Recombinant DNA Technology and Cell Line Development. Medium Development. Cell Metabolism. Protein Glycosylation. Cell Culture Bioreactors. Aeration, Mixing and Hydrodynamics in Bioreactors. Instrumentation and Process Control. Cell Culture Kinetics and Modeling. Fed-Batch Cultivation Of Mammalian Cells For Production Of Recombinant Proteins. Optimization of High Density Perfusion Bioreactors. Cell Separation And Product Capture. Downstream Processing. Formulation, Finishing, and Packaging. Validation Of Cell Culture Based Processes & Qualification Of Associated Equipment And Facility. Facility Design. Transient Expression of Proteins. Large-Scale Propagation of Insect Cells. Advances In Adult Stem Cell Culture. Ex Vivo Culture Of Hematopoietic And Mesenchymal Stem Cells For Tissue Engineering And Cell-Based Therapies.
Detección de Listeria monocytogenes, Salmonella y Yersinia enterocolitica en carne de res en puntos de venta en México

El objetivo de este trabajo fue detectar la presencia de Listeria monocytogenes, Salmonella y Yersinia enterocolitica en carne de res, mexicana e importada, adquirida en supermercados en tres ciudades de Mexico. Antes de analizar los productos carnicos, se estimo el limite de deteccion usando metodos basados en reaccion en cadena de la polimerasa (PCR), tanto de cultivos bacterianos puros como de carne inoculada. Un total de 90 muestras, mitad mexicanas y mitad importadas, fueron recolectadas en este estudio. Un total de 27.78 % muestras analizadas por PCR fueron positivas a L. monocytogenes, 8.89 % a Salmonella y 28.89 % a Y. enterocolitica. Ninguna de las muestras importadas fue positiva a Salmonella. Las muestras mexicanas presentaron mayor indice de Listeria, Yersinia y Salmonella que las muestras de carne importada, lo que indica un posible riesgo de salud para los consumidores que adquieren este tipo de producto. Estos resultados enfatizan la necesidad de implementar de forma obligatoria programas de prevencion y control, Procedimientos Operativos Estandarizados de Sanitizacion (POE’s- SSOP’s en ingles) y Analisis de Peligros y Puntos de Control Criticos (APPCC- HACCP en ingles) en la industria carnica mexicana.
Nucleotide Diversity of a Nuclear and Four Chloroplast DNA Regions in Rare Tropical Wood Species of Dalbergia in Vietnam: A DNA Barcode Identifying Utility

Five nucleotide regions (a nuclear ribosomal DNA internal transcribed sequence (ITS) and four chloroplast DNA fragments (trnL intron, matK-designed, matK-barcode and psbA-trnH spacer) were used for the assesment nucleotide diversity for the eight woody tree species in genus Dalbergia (Dalbergia oliveri Gamble ex Prain, Dalbergia cochinchinensis Pierre, Dalbergia nigrescens Kurz, Dalbergia tonkinensis Prain, Dalbergia assamica Bentham, Dalbergia entadoides Pierre ex Prain, Dalbergia hancei Bentham and Dalbergia dialoides Pierre Niyomdham) of Vietnam. The number of variable sites for individual loci ranged from 13 (psbA-trnH spacer) to 111 (ITS). The highest divergence region was ITS (7.4 %), followed by psbA-trnH spacer (2.2 %), trnL intron (1.5 %), matK-designed (1.2 %) and matK-barcode (1.3 %). Analyses based on threshold genetic distances, Phylogenetic trees-based and nucleotide character differences methods showed that only nuclear ITS region is the best nucleotide diversity of rare woody tree species in Dalbergia genus. Therefore, it can be used as an identification of eight Dalbergia species.
Regulation of replication initiation and HML silencing in Saccharomyces cerevisiae

The Saccharomyces cerevisiae origin recognition complex (ORC) displays a dual role in silencing and initiation of DNA replication, but requires the contribution of auxiliary factors such as Rap1 or Sum1 for full initiation capacity. In this study, the influence of ORC and factors binding in the vicinity of ORC on origin activity and HML silencing was analysed. The silent mating-type loci HML and HMR of S. cerevisiae contain mating-type information that is permanently repressed. This silencing is mediated by flanking sequence elements, the E- and I-silencers. They contain combinations of binding sites for Rap1, Abf1 and Sum1 as well as for ORC. Together, they recruit other silencing factors, foremost the repressive Sir2/Sir3/Sir4 complex, to establish heterochromatin-like structures at the HM loci. However, the HM silencers exhibit considerable functional redundancy, which has hampered the identification of further silencing factors. Therefore, a synthetic, minimal HML-E silencer (HML-SS ΔI) that lacked this redundancy was constructed during the course of this study. It consisted solely of Rap1 and ORC binding sites and the D2 element, a Sum1 binding site, and all three elements were crucial for minimal HML silencing. This silencer was sensitive to a mutation in RAP1, rap1-12, but less sensitive to orc mutations or sum1Δ. Moreover, deletions of SIR1 and DOT1 led to complete derepression of the HML-SS ΔI silencer. This fully functional, minimal HML-E silencer will therefore be useful to identify novel factors involved in HML silencing. Replication initiation at origins of replication in the yeast genome takes place on chromatin as a template, raising the question how histone modifications, for instance histone acetylation, influence origin firing. Initiation requires binding of ORC to a consensus sequence within origins and of other proteins, for instance Sum1, to recognition sites in the vicinity of ORC to support initiation. Sum1 is part of the Sum1/Rfm1/Hst1 complex that represses meiotic genes during vegetative growth via histone deacetylation by the histone deacetylase (HDAC) Hst1. In this study, it was found that Sum1 functioned in initiation as a component of this complex, implying a role for histone deacetylation in origin activity. Several origins were identified in the yeast genome whose activity depended on both Sum1 and Hst1. Importantly, sum1Δ or hst1Δ caused a significant increase in histone H4 lysine 5 (H4 K5) acetylation levels, but not other H4 acetylation sites, at those origins. Furthermore, mutation of lysines to glutamines in the H4 tail, which imitates the constantly acetylated state, resulted in a reduction of origin activity comparable to that in the absence of Hst1, showing that deacetylation of H4 was important for full initiation capacity of these origins.