Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Roscovitine on Schedule of Divisional Morphogenesis, Basal Bodies Proliferation and Cell Divisions in Tetrahymena thermophila

,. ' Summary. During cell cycle of a ciliate Tetrahymena thermophila the divisions of micro- and macronucleus, cortical morphogenesis and cytokinesis are temporarily coordinated. Cortical morphogenesis begins with proliferation of the new ciliary basal bodies (BBs) within meridional cortical rows of ciliary BBs, and with the local proliferation of BBs, which form the new oral apparatus (OA2), positioned subequatorialy and destined for prospective posterior daughter cell (opisthe). Prior to cytokinesis, two prospective daughter cells are of equal size and show metamery of their cortical patterns. We studied effects of 20 uM roscovitine (an inhibitor of several cyclin-dependent kinases) on the cell cycle progression of T. thermophila. We showed that roscovitine delayed cell division, delayed or arrested macronuclear division and induced increase of cell size and the number of BBs in the cortical rows. The increase in the number of BBs in cortical rows induced cell elongation which was proportional to the increase in cell surface area. There was uncoupling between this BBs_proliferation which is continued during prolonged cell cycle and delayed cytokinesis, what resulted in topological alteration of the respective positions of the OA2 and of the contractile vacuole pores (CVPs). In roscovitine treated cells, the new OA2 was positioned subequatorialy, but the fission zone was shifted posterior to the equatorial plane of the cell and positioned across and in the extreme cases behind of the new OA2. This resulted in the formation of a large proter and small size opisthe. The roscovitine treatment induced a formation of a plethora of phenotypes of postdividing cells. We found that irrespective of changes in divisional morphogenesis induced by roscovitine treatment, all mature BBs were associated with the cdc! 4-like phosphatase. Taken together all these data indicate that during cell cycle of T. thermophila the normal morphology of the daughter cells depends on the proper division of micro- and macronucleus and on temporal control of BBs proliferation along the longitudinal rows, during OA2 stomatogenesis and during selection of BBs involved in differentiation of apical BBs (couplets) and cell division.
Facial expression recognition based on two-step feature histogram optimization

The feature histogram is made of different labels containing information about patterns on a pixel-level. This means that pixels of the same label may come from different parts of a face. There must be some errors between the actual feature histogram and the accurate feature histogram which can completely represent the information of a face image. In order to overcome this shortcoming, we propose a facial expression recognition approach based on two-step features histogram optimization. This method requires two steps. In the first step, features histogram based on local binary patterns, uniform local binary patterns and local gradient coding to be extracted. In the second step, a suitable weight to multiply with features histogram extracted in the first step. And these features are classified by the support vector machine. Experiments show that our approach can obtain a higher recognition rate and maintain the time efficiency.
Cyclonet - a database on cell cycle regulation ?

Cyclonet is a database on cell cycle regulation in eukaryotes. It con tains information about specific genes, proteins ant their complexes, models of cell cycle regulation and results of their analysis, microarray data, literature references and other related resources. Using Cyclonet database we are demonstrating that BioUML and BeanExplorer Enterprise Edition technologies can be used for formal description, visualisation and simulation of complex biological systems. BioUML workbench is used to query and edit the database content as well as for visual modelling. BeanExplorer Enterprise Edition provides web interface for access to Cyclonet database.
Ammoniated babassu palm hay in anglo-nubian goat diets

Leaves of babassu may be used in diets for goats under maintenance, however, it is a low-quality roughage due to its high fiber content. The chemical treatment by ammonia causes reduction in the proportion of the cell wall, in addition to providing non-protein nitrogen for the microbial protein synthesis in the rumen. Babassu palm hay ammoniated with 4% urea (BHAU4%) was evaluated in this study as a substitute for guinea grass hay in the maintenance diets of goats in terms of intake, digestibility in vivo, and the partitioning of energy and nitrogen compounds. Twenty Anglo-Nubian male goats were used in a randomised block design with four treatments (diets containing 0, 33, 66, or 100% BHAU4%) and five replicates (animals/block). The chemical compositions of the feeds, leftovers, faeces, nitrogen and crude energy of the urine were evaluated. In addition, the rumen fluid pH, the rumen N-NH3, and the blood serum urea were evaluated. The digestibility of the dry matter (DM), organic matter (OM), crud protein (CP), neutral detergent fiber (NDFap) and detergent acid (ADFap), corrected for ash and protein, declined (P<0.05) 0.0939, 0.0722, 0.0953, 0.1113, and 0.2666%, respectively, with the 1% inclusion of babassu palm hay in the diet. A negative linear effect (P<0.05) was observed in the ingested nitrogen (N), excretion of N in the urine, retained N, and N balance, with decreases of 0.15711, 0.0225 and 0.1071 g/day and 0.1388%, respectively, per percentage unit of the babassu palm hay included in the diet. The intake and digestibility of the DM and nutrients are reduced with the inclusion of BHAU4% in maintenance diets for goats, with positive nitrogen balance and stability of the ruminal pH and N-NH3 as well as blood urea, which presented values within the normal physiological range for goats.
Implication of Endothelin-2 and Oxidative Stress Biomarkers in EssentialHypertension

Objectives: Essential hypertension (EH) is a multifactorial disease and oxidative stress has been demonstrated to play an important role in its pathophysiology. Among several genes implicated in the pathophysiology of hypertension, endothelin-2 (ET-2), a potent vasoconstrictor, has been incriminated as a candidate gene for essential hypertension. To find out correlation of in vivo biomarkers of oxidative stress [oxidized low density lipoprotein (Ox-LDL), 8-iso- Prostaglandin F2α (8-iso-PGF2α)] and Nitrite (NO2-) are related to endothelin-2 levels and to determine their value as diagnostic markers in essential hypertension. Methods: 100 subjects with essential hypertension and 150 age and sex-matched normotensive controls were enrolled to determine levels of ET-2, Ox-LDL, 8-iso-PGF2α and NO2-. Results: We observed significantly augmented levels of ET-2 (P<0.001), Ox-LDL (P<0.001), 8-iso-PGF2α (P<0.001) and nitrite (P<0.001) in EH subjects as compared to the normal healthy controls. ET-2 demonstrated a positive and significant correlation with Ox-LDL, 8-iso-PGF2α, and nitrite levels in subjects with EH. ROC curve analysis revealed that all these variables were significantly able to distinguish EH subjects and controls with high sensitivity and specificity. Conclusions: TOur findings highlight implication of these variables as potential markers for the disease and as possible targets for therapeutic management of the essential hypertension.
SHAREDWEALTH: A CRYPTOCURRENCY TO REWARD MINERS EVENLY

Bitcoin [19] is a decentralized cryptocurrency that has recently gained popularity and has emerged as a popular medium of exchange. The total market capitalization is around 1.5 billion US dollars as of October 2013 [28]. All the operations of Bitcoin are maintained in a distributed public global ledger known as a block chain which consists of all the successful transactions that have ever taken place. The security of a block chain is maintained by a chain of cryptographic puzzles solved by participants called miners, who in return are rewarded with bitcoins. To be successful, the miner has to put in his resources to solve the cryptographic puzzle (also known as a proof of work). The reward structure is an incentive for miners to contribute their computational resources and is also essential to the currency's decentralized nature. One disadvantage of the reward structure is that the payment system is uneven. The reward is always given to one person. Hence people form mining pools where every member of the pool solves the same cryptographic puzzle and irrespective of the person who solved it, the reward is shared evenly among all the members of the pool. The Bitcoin protocol assumes that the miners are honest and they follow the Bitcoin protocol as prescribed. If group of selfish miners comes to lead by forming pools, the currency stops being decentralized and comes under the control of the selfish miners. Such miners can control the whole Bitcoin network [29]. Our goal is to address this problem by creating a distinct peer-to-peer protocol that reduces the incentives for the miners to join large mining pools. The central idea is to pay the “runners-up” who come close to finding a proof, thereby creating a less volatile payout situation. The work done by the “runners-up” can be used by other miners to find the solution of proof of work by building upon their work. Once they find the actual solution they have to include the solution of the other miner in order to get rewarded. The benefit of this protocol is that not only the miners save their computational resources but also the reward is distributed among the miners.
Numerical treatment for nonlinear steady flow of a third grade fluid in a porous half space by neural networks optimized

In this paper‎, ‎steady flow of a third-grade fluid in a porous half‎ space has been considered‎. ‎This problem is a nonlinear two-point‎ boundary value problem (BVP) on semi-infinite interval‎. ‎The‎ solution for this problem is given by a numerical method based on the feed-forward artificial‎ neural network model using radial basis activation functions trained with an interior point method‎. ‎Moreover, to confirm the performance of the proposed technique‎, ‎our results are compared with other available ‎results‎. ‎Numerical results demonstrate the validity and‎ ‎applicability of the ‎technique. ‎
A method for generating Gowdy cosmological models

Metodos numericos han sido extensamente usados para investigar modelos de Gowdy, pero solo recientemente se ha propuesto que las tecnicas de generacion de soluciones pueden ser aplicadas en este caso para generar nuevas soluciones. En este trabajo nos concentraremos en los modelos cosmologicos de Gowdy T3 y mostraremos como una transformacion coordenada compleja junto con un cambio complejo de las funciones metricas nos permite aplicar de una manera sencilla las tecnicas de generacion de soluciones que han sido extensamente usadas para soluciones estacionarias axisimetricas
EXPERIENCES IN INTEGRATING SOCIAL LISTENING WITH THE QAW

This paper proposes a novel method to graft “social listening” technique to Quality Attribute Workshop (QAW). QAW is a team facilitation meeting among stakeholders, which was introduced by Software Engineering Institute (SEI). It helps them communicate each other, thereby exposing interactions of quality attributes, and finding new quality attributes which development team may not have surfaced or considered before. Therefore, having all stakeholders in the meeting is very critical in order to cover their quality attribute concerns. In practices, however, inviting real end users to QAW is almost impossible during development phase, even though they are the most important stakeholders whom development them should consider. So we suggest “social listening”, which elicits end users’ voices or thoughts about a product from SNS, such as Twitter, Facebook, and blogs to compensate end user’s absence in QAW.
Effectiveness of manual palpation in the Northern Water Snake, Nerodia sipedon sipedon, as a method to extract gut contents for dietary studies

Dietary studies have valuable implications to natural history, conservation, and management of species. Four known methods are used to determine the presence, absence, and to identify gut contents for dietary studies and include: stable isotope analysis, fecal analysis, gut flushing, and manual palpation. I tested the effectiveness of the latter method in the Common Water Snake, Nerodia sipedon sipedon. Ninety-five individuals were captured using visual encounter surveys (VES) and manually palpated for presence/absence of gut contents. Thirty-seven specimens from the Marshall University Herpetology Museum were dissected and the number of individuals with gut content presence/absence was compared to the manually palpated individuals. Based on the data collected, a Pearson chi-square test was conducted resulting in failure to accept the null hypothesis, X 2 (1, N=135) = 0.7296, p= 0.05. The results suggest manual palpation is a more effective method than dissection of museum specimens and result in significantly different numbers of gut content samples. The study provides the first quantitative information regarding the effectiveness of methodologies used in dietary studies and the most frequently used in snake studies.
Genetic structure and diversity of Rhododendron arboreum (Ericaceae) in protected and harvested forests in northeast India

Rhododendron arboreum is an ecologically and economically important forest tree species in high altitude forests. In the Himalayan region of Northeast India, R. arboreum is extensively harvested as a source of fuelwood while selected forest stands have been set aside as protected forests by tribal groups resulting in a mosaic of harvested and protected forests. I assessed the genetic structure of R. arboreum in these forests using microsatellite markers that were originally developed for R. mettemichii . Out of eight pairs of primers evaluated, three primer pairs showed reliable amplification and polymorphism in R. arboreum . These three primer pairs or loci (RM1D1, RM2D2, and RM9D6) were used in assessing the genetic structure of R. arboreum in protected and harvested forests in the Eastern Himalayan region of Northeast India. Leaf samples collected from over 125 trees in five populations of R. arboreum representing two protected and three harvested forests, 35 different alleles were detected at three loci, with an average of 11.66 alleles per locus. There were no significant differences in number of alleles between populations. Over all populations, the mean observed and expected heterozygosity values were 0.801 and 0.804, respectively. A total of five private alleles were detected in two of the five populations (Pipraw Jang disturbed and Falokchar Mukto disturbed), suggesting private alleles are confined to harvested populations. The values of population differentiation (Fst) ranged between 0.015 and 0.057 with a corresponding gene flow (Nm) values of 15.8 and 4.1 respectively suggesting moderate levels of gene flow among populations. Population differentiation showed a significant correlation with the geographic distance. The high level of heterozygosity suggests a predominantly outcrossing mating system in R. arboreum . Although the level of population differentiation is low, the significant correlation between population differentiation (Fst) and geographic distance within a short distance of about 5 km is striking. In order to capture and maintain maximum genetic diversity, representative populations of R. arboreum distributed throughout its geographic distribution range should be conserved. These populations may serve as a seed source for regeneration of adjacent harvested forests. Further studies are needed to gain insights into the source of these private alleles as well as average distance of gene flow to determine the optimal distance for establishing protected populations of R. arboreum
Cross-Cultural Validity of the “ Classroom Motivational Climate Questionnaire ” : Comparison between French and Spanish Students

The objective of this study was to obtain evidence about the cross-cultural validity of the “Classroom Motivational Climate Questionnaire” (CMCQ), developed recently for Secondary and High-School students. With this purpose, French and Spanish Secondary and High School students’ results were compared. A total of 749 French students formed the group to be compared with the original Spanish sample. To validate the CMCQ, confirmatory factor analyses, reliability and correlation and regression analyses were made. The results showed that CMCQ is a reliable and valid instrument to measure motivational climate in France as in Spain. It allows detecting which learning patterns can to be changed for improvement, and predicts to a large extent the satisfaction level with the teacher. Likewise it showed some existing differences between Spanish and French students in the motivational role attributed to some teacher’s strategies, differences whose theoretical and practical implications are discussed.
Components and Architectural Design of an Autograder System Family

A new automatic grading (autograder) system has been set up to support the Indonesian selection and preparation process of IOI candidates in Indonesia. As interest in programming com- petitions is increasing, we need an autograder system that can be prepared for a specific purpose and is scalable to be able to handle the increasing number of users. Therefore, we have redesigned a system with a new concept and presented it in this paper. The new autograder system consists of interchangeable components to fulfil all kinds of operational purposes. Instead of having a big and complex system, the proposed system will be automatically composed and deployed for specific operational needs. Design, implementation and operation of the autograding and contest manage- ment systems are supported by IOI alumni.
Project-Team Fractales Fractals, Complex Models and Artiﬁcial Evolution

of TCP for an inﬁnite superposition of sources, in which each “source” i of trafﬁc sends “packets” of data continuously. At time t , it sends Z i ( t ) packets. Between two consecutive time instants t and t + 1 , two things may happen: The source i may experience a “loss”, i.e. the ﬂow control mechanisms of TCP detects that a packet sent by the source did not reach its destination. In this case, TCP forces the source to halve the number of packets sent at time t + 1 . If there is no loss, the source increases Z i ( t ) by 1. The durations ( τ ( i ) k ) k ≥ 1 between the time instants k and k + 1 where a given source i experiences losses are modelled by a sequence of independent exponential random variables with parameter λ i . The total trafﬁc Z ( t ) is then the sum of an inﬁnite number of independent sources with varying rates λ i , where ( λ i ) i ≥ 1 is a non-decreasing sequence of positive numbers. We have proved that, under certain conditions, the Hausdorff multifractal spectrum of Z coincides with the one of a Lévy process with Blumenthal–Getoor exponent β = inf { γ ≥ 1; P i ≥ 1
Revisiting genome-wide suggestive regions for daily milk production in Egyptian buffalo using a multi-locus approach

Our initial GWAS for daily milk production in Egyptian buffalo using SNPs has detected several loci with suggestive signals on chromosomes 1, 5, 6 and 27. In the current study, we investigate the use of haplotypes to confirm associations of suggestive genomic regions. A total number of 250 animals were used to estimate average milk yield deviations for the first five lactations. AffyPipe and PLINK were used to check the quality of genotypes. Haplotypes were constructed and partitioned into haplotype blocks using fastPHASE and Haploview, respectively. Association analysis for each suggestive region was performed using PLINK. Our results confirmed the previous findings of genome-wide suggestive signals on the four chromosomes after Bonferroni correction. The most significant haplotype (P=3.9 x 10-05) was identified on chromosome 5 between 72.54 and 72.57 Mb, while the most significant SNP from our initial investigation was located on chromosome 27 (P=1.87 x 10-6) at 30.14 Mb. The identified regions were subjected to enrichment analysis using the GeneCodis databases. The analysis ranked immune response at the top of all biological processes (P≤0.001). In conclusion, multi-markers based approach can be used beside single markers to identify genomic associations for small population especially for phenotypes with moderate variation. Further investigations of the validated loci in different populations are necessary to identify the potential candidate genes and probably causative mutations.
Solar Energy Potential in Kathmandu Valley, Nepal

Meteorological data such as solar radiation (1975-1984, and 2002-2010) and sunshine duration (1968-2004) were analyzed to study temporal characteristics of solar energy and investigate solar energy potential in Kathmandu valley. Pre-monsoon and post monsoon seasons have higher mean monthly sunshine duration (about 8 hours/day) than summer (about 5 hours/day) and winter (about 7 hours/day) seasons over Kathmandu. Pre-monsoon and monsoon seasons receive solar energy of about 190 Wm -2 and 170 Wm -2 respectively. The winter season receives the least amount of solar radiation (135 Wm -2 ). Approximately 220 MW of solar electricity can be produced in Kathmandu that will substantially fulfill current energy demand and reduce environmental pollution in the valley by replacing fossil fuels with clean solar electricity. Journal of Hydrology and Meteorology, Vol. 8(1) 2012, p.77-82
A Satellite Multibeam Antenna for High-Rate Data Relays

This paper presents recent progress on a novel multibeam antenna that is designed to serve as a key component for multiple transparent real-time capable data relays linking several LEO satellites to a single earth station via a GEO satellite. High data rates up to several hundreds of Mbit/s can be achieved. The proposed antenna operates at about 26 GHz and can track LEO satellites by switching between different beams and applying digital beamforming to each beam.
A Dog’s Brexit for UK Food Manufactures, in terms of Food Safety Culture

The UK’s political toxicity, miscommunication and lack of progress associated with Brexit, has united both the leavers and the retainer camps, in the sense that they urgently require clarity and confidence concerning the government’s negotiation strategy. This paper critically discusses current Brexit progress and its effects on UK food manufacturers in terms of their food safety cultural compliance. A mixed method approach was adopted via four focus groups populated with 20 senior/middle managers from 10 UK food manufacturers. The data extracted reflects food manufacturers’ nervousness about Post Brexit, areas of food safety cultural best practice and its current challenges. The paper concludes with four key themes for consideration which food manufacturers are encouraged to reflect upon and in doing so, provides a valuable contribution to community of practice in food manufacturing.
The relationship between teachers’ psychological well-being and their quality of school work life

The purpose of this study was to investigate the relationship between quality of school work life (QSWL) and psychological well-being (PWB) of public school teachers. Cluster random sampling technique was used to collect data from 784 teachers in 120 schools across six providences in Turkey. Data were primarily collected with two validated scales: Psychological Well-being, and the Quality of School Work Life. The study revealed that teachers rated their level of Quality of School Work Life moderately, and rated their level of Psychological Well-being relatively higher. The findings of the study indicate that there were some differences in QSWL levels of teachers in connection with some demographic variables. Further, stepwise linear regression revealed that their Quality of School Work Life rating described teachers’ Psychological Well-being. The findings are compared with previous research, and finally, the limitations of the study are discussed with recommendations for future research.