Miracle-dz/UniFAR-FTUs huggingface dataset:
Scale Construction of The Discipline Scale For Indonesian Air Force (TNI Angkatan Udara) Military Personnel (IMPI-D32)

This study aims to create a discipline measuring tool with good psychometric properties for Indonesian Air Force Military Personnel (IMPI-D32). It was conducted using an integration of self-discipline, motivation to obey, and discipline theories in a military context. The IMPI-D32 consists of 32 items and its construction are based on the Classical Theory Test. The item's content validity was estimated using the Aiken V index at a minimum of . 690. Furthermore, the item discriminations showed that the corrected item-total correlation for all items is above . 3. Meanwhile, the reliability estimation with the Spearman-Brown formula indicated a satisfactory coefficient value of . 916. The standard error of measuring the IMPI-D32 was 3.439 with the confidence of true score interval between X-6 to X+6.
VSC-HVDC Transmission with Cascaded Two-Level Converters

SUMMARY The two-level converter valve together with series-connected press-pack insulated-gate bipolar transistors (IGBTs) is a reliable and proven technology for transmission-scale converters. This has been put to use in a further development of high-voltage dc (HVDC) transmission employing voltage-source converters (VSCs). A cascaded two-level (CTL) converter consisting of several smaller two-level building blocks, also called cells, enables the creation of a nearly sinusoidal output voltage from the converter. The development of the CTL is the subject for this paper. Using technology modules developed and refined during the last 15 years, it has been possible to create a converter that addresses and solves many of the limitations of VSC-HVDC transmission while retaining all operational functionality. The technology is scalable up to the highest transmission voltages. Losses are reduced to roughly 1% per converter through a combination of methods. A method for removing the need for external protective equipment and circuitry in the valves is presented. The main control of the converter has been reused, although the control bandwidth, i.e., the speed, has been further improved.
Endothelial Cell-Derived Extracellular Vesicles Target TLR4 via miRNA-326-3p to Regulate Skin Fibroblasts Senescence

Backgrounds Skin aging could be regulated by the aberrant expression of microRNAs. In this manuscript, we explain that endothelial cell-derived extracellular vesicles could act as supporters to deliver exogenous miR-326-3p to accelerate skin fibroblasts senescence. Methods β-galactosidase senescence staining assay, Hoechst 33258 apoptosis staining assay, and Ki67 staining assay were used to evaluate the biological function of mouse skin fibroblasts. Real-time PCR was applied to assay miRNAs and mRNAs expressions. Western blot was used to detect TLR4 protein expression. The target gene of miRNA were identified using a double luciferase reporter assay. miR-326-3p mimic/inhibitor and siRNA-TLR4 can demonstrate a nonnegligible link between miR-326-3p-TLR4 and skin aging. Results In coculture experiment, senescence endothelial cells could promote the skin fibroblasts senescence and apoptosis via extracellular vesicles pathway. In contrast, miR-326-3p mimics accelerated senescence and apoptosis of skin fibroblasts, while miR-326-3p inhibitor could dramatically delay skin fibroblasts senescence and apoptosis. TLR4 was proved to be a miR-326-3p directly target gene via double luciferase assay. After skin fibroblasts transfected with siRNA-TLR4, cellular senescence and apoptosis were significantly increased. Furthermore, the skin tissues of aging mice were shown with overexpression of miR-326-3p and decrease of TLR4 gene and protein expression levels. Conclusions Endothelial cell-derived extracellular vesicles delivery of miR-326-3p was found to have a function in skin fibroblasts via target TLR4. Therefore, endothelial cell-derived extracellular vesicles in antiaging therapies might be a new treatment way for delaying skin aging.
A 16S rRNA Gene-Based Metabarcoding of Phosphate-Rich Deposits in Muierilor Cave, South-Western Carpathians

Muierilor Cave is one of Romania’s most important show caves, with paleontological and archeological deposits. Recently, a new chamber was discovered in the cave, with unique yellow calcite crystals, fine-grained crusts, and black sediments. The deposits in this chamber were related to a leaking process from the upper level that contains fossil bones and a large pile of guano. Samples were taken from the new chamber and another passage to investigate the relationship between the substrate and microbial community. Chemical, mineralogical, and whole community 16S rRNA gene-based metabarcoding analyses were undertaken, and the base of the guano deposit was radiocarbon dated. Our study indicated bacteria linked to the presence of high phosphate concentration, most likely due to the nature of the substrate (hydroxyapatite). Bacteria involved in Fe, Mn, or N cycles were also found, as these elements are commonly identified in high concentrations in guano. Since no bat colonies or fossil bones were present in the new chamber, a high concentration of these elements could be sourced by organic deposits inside the cave (guano and fossil bones) even after hundreds of years of their deposition and in areas far from both deposits. Metabarcoding of the analyzed samples found that ∼0.7% of the identified bacteria are unknown to science, and ∼47% were not previously reported in caves or guano. Moreover, most of the identified human-related bacteria were not reported in caves or guano before, and some are known for their pathogenic potential. Therefore, continuous monitoring of air and floor microbiology should be considered in show caves with organic deposits containing bacteria that can threaten human health. The high number of unidentified taxa in a small sector of Muierilor Cave indicates the limited knowledge of the bacterial diversity in caves that can have potential applications in human health and biotechnology.
Impact of Foreign Direct Investment, Rising Oil Prices, and Industry Value Added on Economic Growth of Pakistan

Rising oil prices, foreign direct investment, and industrial value-added are the key component that affects the economic stature of a country. This study locates the relationship between FDI, rising oil prices, and industry value added on the economic growth of Pakistan. To do the analyses, data length ranging from 1980 to 2020 was used. For the regression results, the analytical technique of Autoregressive Distributed Lag (ARDL) is incorporated. The empirical results show that FDI encourages economic growth. Nevertheless, industry value added is also located for positive affectation on economic growth. However, rising oil prices are found to have negative effects on economic growth. Similarly, the combined effect of oil prices and FDI are found discouraging for economic growth. As a policy option, the government of Pakistan must need to encourage FDI inflows and should step up toward industrialization. On the other side, rising oil prices be controlled at the maximum extent as it is damaging to economic prosperity.
Noninvasive Prenatal Testing in Immunohematology—Clinical, Technical and Ethical Considerations

Hemolytic disease of the fetus and newborn (HDFN), as well as fetal and neonatal alloimmune thrombocytopenia (FNAIT), represent two important disease entities that are caused by maternal IgG antibodies directed against nonmaternally inherited antigens on the fetal blood cells. These antibodies are most frequently directed against the RhD antigen on red blood cells (RBCs) or the human platelet antigen 1a (HPA-1a) on platelets. For optimal management of pregnancies where HDFN or FNAIT is suspected, it is essential to determine the RhD or the HPA-1a type of the fetus. Noninvasive fetal RhD typing is also relevant for identifying which RhD-negative pregnant women should receive antenatal RhD prophylaxis. In this review, we will give an overview of the clinical indications and technical challenges related to the noninvasive analysis of fetal RBCs or platelet types. In addition, we will discuss the ethical implications associated with the routine administration of antenatal RhD to all pregnant RhD-negative women and likewise the ethical challenges related to making clinical decisions concerning the mother that have been based on samples collected from the (presumptive) father, which is a common practice when determining the risk of FNAIT.
Does Government Expenditure Affect Poverty, Health, and Education? Evidence from Aceh, Indonesia

Economists have talked about government expenditure and its relation to poverty, health, and education for decades. Indeed, many theories and empirical evidence have been conducted since then. This study evaluates the relationship between Special Autonomy Fund (SAF) and poverty, health, and education indicators in Aceh province, Indonesia, using a panel dataset of 30 regions in the 2002-2018 period. Synthetic Control Method (SCM) is used as the model to accommodate the allocation of SAF to Aceh given by the central government since it is commonly applied to the cases of policy intervention in comparative case studies. This paper discovers that the SAF plays a vital role in lowering the poverty rate, escalating access to safe sanitation, and improving the net enrollment ratio of senior secondary schools. However, there is no prominent association between SAF allocation and access to safe water. This outcome variable shows positive and negative signs; therefore, a conclusion could not be provided.
Antihyperthermic Treatment in the Management of Malignant Infarction of the Middle Cerebral Artery

Malignant infarction of the middle cerebral artery (m-MCA) is a complication of ischemic stroke. Since hyperthermia is a predictor of poor outcome, and antihyperthermic treatment is well tolerated, our main aim was to analyze whether the systemic temperature decrease within the first 24 h was associated with a better outcome. Furthermore, we studied potential biochemical and neuroimaging biomarkers. This is a retrospective observational analysis that included 119 patients. The temperature variations within the first 24 h were recorded. Biochemical laboratory parameters and neuroimaging variables were also analyzed. The temperature increase at the first 24 h (OR: 158.97; CI 95%: 7.29–3465.61; p < 0.001) was independently associated with a higher mortality. Moreover, antihyperthermic treatment (OR: 0.08; CI 95%: 0.02–0.38; p = 0.002) was significantly associated with a good outcome at 3 months. Importantly, antihyperthermic treatment was associated with higher survival at 3 months (78% vs. 50%, p = 0.003). Significant independently associations between the development of m-MCA and both microalbuminuria (OR: 1.01; CI 95%: 1.00–1.02; p = 0.005) and leukoaraiosis (OR: 3.07; CI 1.84–5.13–1.02; p < 0.0001) were observed. Thus, antihyperthermic treatment within the first 24 h was associated with both a better outcome and higher survival. An increased risk of developing m-MCA was associated with leukoaraiosis and an elevated level of microalbuminuria.
Slack Potassium Channels Modulate TRPA1-Mediated Nociception in Sensory Neurons

The transient receptor potential (TRP) ankyrin type 1 (TRPA1) channel is highly expressed in a subset of sensory neurons where it acts as an essential detector of painful stimuli. However, the mechanisms that control the activity of sensory neurons upon TRPA1 activation remain poorly understood. Here, using in situ hybridization and immunostaining, we found TRPA1 to be extensively co-localized with the potassium channel Slack (KNa1.1, Slo2.2, or Kcnt1) in sensory neurons. Mice lacking Slack globally (Slack−/−) or conditionally in sensory neurons (SNS-Slack−/−) demonstrated increased pain behavior after intraplantar injection of the TRPA1 activator allyl isothiocyanate. By contrast, pain behavior induced by the TRP vanilloid 1 (TRPV1) activator capsaicin was normal in Slack-deficient mice. Patch-clamp recordings in sensory neurons and in a HEK cell line transfected with TRPA1 and Slack revealed that Slack-dependent potassium currents (IKS) are modulated in a TRPA1-dependent manner. Taken together, our findings highlight Slack as a modulator of TRPA1-mediated, but not TRPV1-mediated, activation of sensory neurons.
Inclusive Learning for Children in Northeast Nigeria: Radio School Response During a Global Pandemic

With a burgeoning out‐of‐school population and illiteracy rate, the situation of protracted conflict and crises fuelled by the Boko‐Haram insurgency further exacerbates educational inequality for children in northern Nigeria. The Covid‐19 pandemic further deepened the “educational poverty” experienced there. This article focuses on data generated around ACE radio school, an initiative to mitigate the impact of Covid‐19‐related school closures in northern Nigeria. The initiative targeted young learners using radio as a medium to support their continued learning remotely in numeracy, literacy, sciences, and civics education. Daily learning activities were broadcasted in the local Hausa language, supported through “listening groups” that engaged local learning facilitators in the communities. Despite the known existing barriers that have been identified to hinder access to quality education in the region, including poverty, religion, socio‐cultural factors, and protracted conflict situations, our interviews revealed that parents were committed to supporting their children’s attendance at listening groups, due to the use of their mother tongue as a mode of instruction. Drawing on a conversational learning approach, we argue that understanding local conditions and adopting local solutions, such as the radio lessons delivered in these children’s mother tongue, have implications for enhancing improved learner outcomes in marginalised contexts.
Tendon Healing Response Is Dependent on Epithelial–Mesenchymal–Tendon Transition State of Amniotic Epithelial Stem Cells

Tendinopathies are at the frontier of advanced responses to health challenges and sectoral policy targets. Cell-based therapy holds great promise for tendon disorder resolution. To verify the role of stepwise trans-differentiation of amniotic epithelial stem cells (AECs) in tendon regeneration, in the present research three different AEC subsets displaying an epithelial (eAECs), mesenchymal (mAECs), and tendon-like (tdAECs) phenotype were allotransplanted in a validated experimental sheep Achilles tendon injury model. Tissue healing was analyzed adopting a comparative approach at two early healing endpoints (14 and 28 days). All three subsets of transplanted cells were able to accelerate regeneration: mAECs with a lesser extent than eAECs and tdAECs as indicated in the summary of the total histological scores (TSH), where at day 28 eAECs and tdAECs had better significant scores with respect to mAEC-treated tendons (p < 0.0001). In addition, the immunomodulatory response at day 14 showed in eAEC-transplanted tendons an upregulation of pro-regenerative M2 macrophages with respect to mAECs and tdAECs (p < 0.0001). In addition, in all allotransplanted tendons there was a favorable IL10/IL12 compared to CTR (p < 0.001). The eAECs and tdAECs displayed two different underlying regenerative mechanisms in the tendon. The eAECs positively influenced regeneration mainly through their greater ability to convey in the host tissue the shift from pro-inflammatory to pro-regenerative responses, leading to an ordered extracellular matrix (ECM) deposition and blood vessel remodeling. On the other hand, the transplantation of tdAECs acted mainly on the proliferative phase by impacting the density of ECM and by supporting a prompt recovery, inducing a low cellularity and angle alignment of the host cell compartment. These results support the idea that AECs lay the groundwork for production of different cell phenotypes that can orient tendon regeneration through a crosstalk with the host tissue. In particular, the obtained evidence suggests that eAECs are a practicable and efficient strategy for the treatment of acute tendinopathies, thus reinforcing the grounds to move their use towards clinical practice.
The Involvement of Alarmins in the Pathogenesis of Sjögren’s Syndrome

Sjögren’s syndrome (SS) is a chronic autoimmune disease that affects exocrine glands, primarily the salivary and lachrymal glands. It is characterized by lymphoplasmacytic infiltration of the glandular tissues, ultimately leading to their dysfunction and destruction. Besides classic dry eyes and dry mouth defined as sicca syndrome, patients affected by the disease also typically display symptoms such as fatigue, pain and in more than 50% of cases, systemic manifestations such as arthritis, interstitial lung involvement, neurological involvement and an increased risk of lymphoma. The pathophysiological mechanisms underlying SS still remain elusive. The crucial role of innate immunity has been advocated in recent years regarding the pathogenesis of pSS, especially in the initiation and progression toward autoimmunity. Alarmins are endogenous molecules that belong to the large family of damage associated molecular pattern (DAMP). Alarmins are rapidly released, ensuing cell injury and interacting with pattern recognition receptors (PRR) such as toll-like receptors (TLR) to recruit and activate cells of the innate immune system and to promote adaptive immunity responses. This review highlights the current knowledge of various alarmins and their role in the pathogenesis of pSS.
DETECTING AND ANALYZING COPY NUMBER ALTERNATIONS IN ARRAY-BASED CGH DATA

Copy number changes or alterations are a form of genetic variation in the human genome. Genomic DNA copy number alterations (CNAs) are associated with the development and progression of cancers. Array-based comparative genomic hybridization (a-CGH) is a technique used to identify copy number changes in genomic DNA. It yields data consisting of fluorescence intensity ratios of test and reference DNA samples. The intensity ratios provide information about the number of copies in DNA. Practical issues such as the contamination of tumor cells in tissue specimens and normalization errors necessitate the use of automated statistics algorithms for learning about the genomic alterations from array CGH data. Specifically, there is a need for algorithms that can identify gains and losses in the number of copies based on statistical considerations, rather than merely detect trends in the data. For this purpose the proposed study introduces three different approaches; Circular binary segmentation, Bayesian approach, .
Noise factor of active-tile for two-dimensional communication

This paper presents theoretical analysis and empirical evaluation of noise factor of active-tile two-dimensional communication (2DC) system. 2DC uses a thin waveguide sheet for microwave propagation along the sheet. For implementing a room-scale 2DC, an active tile, which compensates signal loss with an active circuit and enables signal propagation across a number of sheets, has been proposed in our previous work. Although the signal strength is kept constant across the tiles, signal-to-noise ratio (SNR) decreases due to the noise factor of amplifiers. It determines the maximum number of 2DC tiles connected in series while providing a required SNR. The paper shows SNR formulation using the noise factor of each active tile and the number of tiles in a series connection. Based on it, the maximum number of tiles connected in series with a required overall SNR is determined.
Development of a 100kW, 20 kHz Nanocrystalline Core Transformer for DC / DC Converter Applications

Achievement of high power levels in a single module inverter/converter application always has number of advantages. However, high frequency magnetic components most often become the limiting factor in the achievement of such high power levels in a single module. In this paper the achievement of 100kW in 20kHz transformer is discussed. This development is an extension of a series of developments carried out with high frequency, high power transformers of power and frequency levels such as 40kW, 20kHz and 50kW, 50kHz. The advantages and disadvantages of Ferrite and Nanocrystalline core materials at this power levels are discussed and the choice of Nanocrystalline core is explained. Further naval winding technique that mitigates high frequency conductor losses is presented in this paper with a theoretical explanation on its advantages.
From conceptual models to safety assurance : applying model-based techniques to support safety assurance

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Immunogenicity and Safety of Homologous and Heterologous Prime–Boost Immunization with COVID-19 Vaccine: Systematic Review and Meta-Analysis

A prime–boost strategy of COVID-19 vaccines brings hope to limit the spread of SARS-CoV-2, while the immunogenicity of the vaccines is waning over time. Whether a booster dose of vaccine is needed has become a widely controversial issue. However, no published meta-analysis has focused on the issue. Therefore, this study assessed the immunogenicity and safety of the different combinations of prime–boost vaccinations. Electronic databases including PubMed, the Cochrane Library, Embase, medRxiv, Wanfang and CNKI were used to retrieve the original studies. A total of 28 studies, 9 combinations of prime–boost vaccinations and 5870 subjects were included in the meta-analysis, and random effect models were used to estimate pooled immunogenicity and safety. The immunity against COVID-19 after the prime vaccination waned over time, especially in the populations primed with inactivated vaccines, in which the seropositive rate of antibodies was only 28% (95% CI: 17–40%). Booster vaccination could significantly increase the antibody responses, and heterologous immunization was more effective than homologous immunization (neutralization titers: 1.65 vs. 1.27; anti-RBD IgG: 1.85 vs. 1.15); in particular, the combination of inactivated–mRNA vaccines had the highest antibody responses (neutralization titers: MRAW = 3.64, 95% CI: 3.54–3.74; anti-RBD IgG: 3.73, 95% CI: 3.59–3.87). Moreover, compared with the initial two doses of vaccines, a booster dose did not induce additional or severe adverse events. The administration of the booster dose effectively recalled specific immune responses to SARS-CoV-2 and increased antibody levels, especially in heterologous immunization. Considering the long-term immunogenicity and vaccine equity, we suggest that now, only individuals primed with inactivated vaccines require a booster dose.