Miracle-dz/UniFAR-FTUs huggingface dataset:
SlKIX8 and SlKIX9 are conserved repressors of organ size in the asterid species tomato

Plant organ size and shape are major agronomic traits that depend on cell division and expansion, which are both regulated by complex gene networks. In several eudicot species belonging to the rosid clade, organ growth is controlled by a repressor complex consisting of PEAPOD (PPD) and KINASE-INDUCIBLE DOMAIN INTERACTING (KIX) proteins. Whether the function of these proteins as regulators of organ size is conserved in asterids as well, which together with the rosids constitute most of the core eudicot species, is still unknown. We used CRISPR-Cas9 genome editing to target SlKIX8 and SlKIX9 in the asterid model species tomato ( Solanum lycopersicum ) and analyzed loss-of-function phenotypes. We found that loss of function of SlKIX8 and SlKIX9 led to the production of enlarged, dome-shaped leaves and that these leaves exhibited increased expression of putative SlPPD target genes. Moreover, kix8 kix9 mutants carried bigger fruits with increased pericarp thickness. Protein interaction assays also confirmed that SlKIX8 and SlKIX9 act as molecular bridges between the SlPPD and SlTOPLESS co-repressor proteins. Our results show that KIX8 and KIX9 are conserved regulators of organ size in distinct eudicot species and can thus provide strategies to improve yield in fruit crops.
Retrospective Cohort Study on Acute Care in Obstetrics and Gynecology: Analogies and Differences When Compared to Emergency Medicine

Introduction: the demand for urgent obstetric and gynecology care has progressively increased: in the United States approximately 1.4 million gynecologic visits are made to the emergency department (ED) annually, while almost 75% of women make at least 1 unscheduled visit during pregnancy. Moreover, research has recently focused on setting standards in unscheduled care, and developing quality indicators to improve patients’ health. Therefore, we investigated the characteristics of women with acute gynecological or pregnancy complaints using quality indicators developed for emergency medicine, to better define the needs of this population and improve care. Methods: Retrospective cohort study on ED, and Obstetrics and Gynecology (ObGyn) triage visits, at a tertiary care hospital in Italy, during 2012. Data were analyzed with population-averaged logistic regression and Poisson regression. Results: When compared to the 33,557 ED visits, the 9245 ObGyntriage referrals were more frequently associated with pregnancy (≤12 weeks’ gestation, OR: 30.7, 95%CI; 24.5 - 38.4; >12 weeks’ gestation, OR 81.2, 95%CI; 64.8 - 101.4), vaginal bleeding (OR 156.6, 95%CI; 82.7 - 294.4), diurnal (night access OR 0.87, 95% CI; 0.78 - 0.96) and weekday access (holiday access OR 0.87, 95%CI; 0.78 - 0.95), frequent users (recurrent ED visits IRR 0.87, 95%CI; 0.83 - 0.9) and lower hospital admissions (ED admission OR 1.6, 95%CI; 1.4 - 1.8). Conclusion: ObGyn triage patients differed from ED users, and were at higher risk of “crowding”. Such diversities should be considered to improve female healthcare services and allocate resources more efficiently.
The complete chloroplast genome of Korean Gastrodia elata Blume

Abstract The completed chloroplast genome of Gastrodia elata Blume (G. elata) from Korea was determined in this study. The cpDNA is 35,230 bp in length and lacked the large and small single copy (LSC and SSC) regions, due to the lost inverted repeat (IR). The overall AT content is 73.30%, and the cpDNA contains 20 protein-coding genes, 5 tRNA genes, and 3 rRNA genes. Remarkably, the Korean G. elata cp genome was 74 bp smaller than that of the Chinese G. elata. It revealed substantial sequence variants 495 SNPs and 75 InDels between the two G. elata genomes.
Found: a missing breeding ground for endangered western North Pacific humpback whales in the Mariana Archipelago

Humpback whales Megaptera novaeangliae that breed in the western North Pacific (WNP) are listed as endangered under the US Endangered Species Act. Previous research in the WNP concluded that the full extent of humpback whale breeding areas is unknown. Recovering this endangered population requires identifying all associated breeding grounds and potential threats in those locations. Prior to 2015, humpback whales were known to occur in the Mariana Archipelago (within the WNP), but their population identity and habitat use there were unknown. To determine the population identity of humpback whales in the Mariana Archipelago and whether the area serves as a breeding ground for these whales, small-boat photo-identification and biopsy sampling surveys were conducted in the southern portion of the archipelago during February and March 2015−2018. A total of 14 mother−calf pairs and 27 other non-calf whales were encountered. Seven non-calves were re-sighted in multiple years, including 4 females associated with calves in one or more years. Competitive behavior was observed in multiple years. Comparisons with other North Pacific humpback whale catalogs resulted in matches to breeding (Japan and Philippines) and feeding (Russia) grounds in the WNP. DNA profiling of 28 biopsy samples identified 24 individuals (14 females, 10 males) representing 7 mitochondrial DNA haplotypes. The haplotype frequencies from the Mariana Archipelago showed the greatest identity with the Ogasawara breeding ground and Commander Islands feeding ground in the WNP. This study establishes the Mariana Archipelago as a breeding area for endangered WNP humpback whales, which should be considered in ongoing research and conservation efforts.
Effect of Radiofrequency Electromagnetic Radiation on Photobacterium phosphoreum Luminescence

Background: The technological progress has led to the widespread use of various radiofrequency electromagnetic radiation (RF-EMR) sources. Luminous bacteria were used as test objects for research of radio waves influence on living organisms. Objective: The presented study was focused on the processes related to Photobacterium phosphoreum luminescence under RF-EMR: some physiological, biochemical consequences and the variations in luxb gene expression. Material and Methods: The IMV B-7071 strain of the luminous marine bacterium P. phosphoreum from the culture collection of the D.K. Za bo lotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine was used as an object of the study. We used “UHF-62”, “Ray-11” and “MRTA-02” commercial devices as a source of RF-EMR. Results: It has been revealed that RF-EMR affected luminescence intensity, transcriptional activity of luciferase encoding gene, superoxide dismutase activity, cell survival rate. It was found that inhibition or stimulation of P. phosphoreum IMV B-7071 luminescence intensity depends on exposure duration. Conclusion: The data indicated the stressful nature of the RF-EMR action. Results obtained in this study suggest that luminescence intensity of P. phosphoreum IMV B-7071 bacterial cells is an indicator of the RF-EMR biotropic impact. The marked dependence demonstrated the effects of stimulation and inhibition of luminescence intensity under RF-EMR influence on bacteria. We observed an increase in the intensity of bacterial luminescence after 5 min of exposure for all types of radiation. While 15 min exposure in the case of 15 W power VHF EMR and UHF EMR resulted in a significant decrease in the luminescence intensity. These data demonstrate significant changes in the luminescence intensity under the action of 15 W power due to heating of the medium. Since the most pronounced effects were observed during the 5 and 15 min action of EMR exposure, further studies were focused on these values of the exposure duration.
Developing a Rational Peptide Ligand Design Method Using CD13 as a Prototype Target Receptor

By Md Zahir Uddin University of the Pacific 2019 Structure based computational peptide design methods have gained significant interest in recent years with the availability of structural insights of protein-protein interactions obtained from the crystal structures. Most of these approaches design new peptide ligands by connecting the crucial amino acid residues from the protein interface and are generally not based on any predicted receptor-ligand interaction. In this work, a peptide design method based on the KnobSocket model was used to identify the specific ligand residues packing into the receptor interface. This method enables rational peptide ligand design by predicting amino acid residues that will fit best at the binding site of the receptor protein. Specific peptide ligands for the model receptor CD13 that are overexpressed in several cancer types were designed in this study. From the initial library of designed peptides, three potential candidates were selected based on simulated energies in the CD13 binding site using the programs Molecular Operating Environment (MOE) and AutoDock Vina. In the CD13 enzymatic activity inhibition assay, the three identified peptides exhibited 2.7 to 7.4 times lower IC50 values (GYPAY, 227 μM; GFPAY, 463 μM; GYPAVYLF, 170 μM) when compared to the known peptide ligand CNGRC(C1-C5) (1260 μM). The binding affinities of the peptides (GYPAY, Ki = 54.0 μM; GFPAY, Ki = 74.3 μM; GYPAVYLF, Ki = 38.8 μM) were 10 to 20 times higher than that of CNGRC (C1-C5) (Ki = 773 μM). The double reciprocal plots from the steady state enzyme
Leadership power, preference homogeneity, and legislative party conflict*

ABSTRACT Over the past few decades, there has been a considerable increase in the level of partisan conflict. What explains the extent of partisan conflict experienced in legislatures? To examine this question, we develop aggregate-level measures of inter-party conflict from roll-call voting data on procedural and final passage votes during the 1999–2000 and 2003–2004 sessions to examine the mechanisms of polarisation in U.S. state legislatures. We systematically model inter-party conflict as a function of institutional rules and preferences. Our results suggest that the level of inter-party conflict (i.e. party polarisation) in legislative voting is largely related to the homogeneity of members’ electorally-induced preferences. However, we also uncover interesting institutional effects. Although minority party rights is the only institutional variable associated with inter-party conflict on final passage votes, majority party leaders’ committee appointment rights, committees’ discretion in reporting bills to the floor, and minority party rights each are strongly associated with inter-party conflict on procedural roll-call votes.
Potential Benefits of Discrete-Time Controllerbased Treatments over Protocol-based Cancer Therapies

In medical practice, the effectiveness of fighting cancer is not only determined by the composition of the used drug, but determ ined by the administration method as well. As a result, having drugs with a suitable action profile is just a promising beginning, but without appropriate delivery method s , the therapy still can be ineffective. Finding the optimal biologic dose is an empir ical process in medical practice; however, using controllers, an automated optimal administration can be determined . In this paper , we evaluate the effectiveness of different drug delivery protocols; using in silico simulations (like bolus dose s, low - dose metron omic regimen and continuous infusion therapy ). In addition, we compare these results with discrete - time controller - based treatments containing state feedback, setpoint control, actual state observer and load estimation.
Effective medical ozone production using mesh electrodes in double dielectric barrier type plasma generators

One of the most effective ways to produce ozone is to use Dielectric barrier discharge plasma (DBDP). Various geometric configurations can be used to produce ozone. However, it is necessary to develop a reactor to produce medical ozone. Medical ozone can be produced using Double DBD reactor with an oxygen source. The aim of this paper is to carry out an experimental analysis of ozone concentrations which produced using DDBD reactor. The reactor used cylindrical- cylindrical electrode configurations made from copper mesh. The high AC voltage was applied in the range between 0,5 to 3,2 kV and the frequency of 50 Hz. Oxygen (O2) is used as input gas source with several variations in flow rates from 0,2 to 1 liter/min. The results show that air flow rate input and voltage can affect ozone concentration. Ozone concentration can be increased by increasing the voltage or decreasing the flow rate. The results also showed that DDBD with this configuration could produce medical ozone concentration from 24 – 768 ppm which could be applied further in the medical field.One of the most effective ways to produce ozone is to use Dielectric barrier discharge plasma (DBDP). Various geometric configurations can be used to produce ozone. However, it is necessary to develop a reactor to produce medical ozone. Medical ozone can be produced using Double DBD reactor with an oxygen source. The aim of this paper is to carry out an experimental analysis of ozone concentrations which produced using DDBD reactor. The reactor used cylindrical- cylindrical electrode configurations made from copper mesh. The high AC voltage was applied in the range between 0,5 to 3,2 kV and the frequency of 50 Hz. Oxygen (O2) is used as input gas source with several variations in flow rates from 0,2 to 1 liter/min. The results show that air flow rate input and voltage can affect ozone concentration. Ozone concentration can be increased by increasing the voltage or decreasing the flow rate. The results also showed that DDBD with this configuration could produce medical ozone concentration from 24 – 768 ppm.
Coping with mental health conditions at work and its impact on self-perceived job performance

PurposeThis exploratory qualitative study examines both the impact of mental health conditions on self-perceived job performance and how individuals with mental health conditions cope with their conditions at work.Design/methodology/approachA total of 257 responses to a qualitative questionnaire and 17 in-depth interviews with individuals with mental health conditions are analyzed.FindingsThe findings show that mental health conditions can negatively impact self-perceived job performance in the form of lower quality of one's work, slower pace, and more mistakes. In addition, the findings reveal coping strategies that positively and negatively affect one’s performance at work. Strategies that negatively influence one’s performance include substance abuse and self-harm, suppressing and hiding one's symptoms, and forcing oneself to continue to work when feeling unwell. Coping strategies that tend to positively affect their performance include accepting one's condition and taking time off, medication and counseling, mindfulness activities, transparent communication, humor, and a compensation strategy.Originality/valueA growing number of individuals struggle with mental health conditions at work, impacting both organizations and employees. However, little is known about the influence of mental health conditions on one's performance at work, how individuals cope with their mental health conditions at work, and what effect those coping strategies have on organization-relevant outcomes.
Influence of teeth resection on the skin temperature and acute phase response in newborn piglets

Two experiments were carried out to determine the effect of different teeth resection methods on skin temperature, concentrations of the acute phase proteins C-reactive protein (CRP) and serum amyloid A (SAA), and cortisol in piglets. In Experiment 1, piglets from 60 litters were assigned to three treatments where the ‘needle’ teeth were clipped (CLIP), ground (GRIND) or left intact (INT) within 12 h of birth; skin temperature was measured immediately afterwards. Fourteen pigs were selected in each treatment for blood sampling at 1 day and 29 days-of-age for the determination of concentrations of CRP, SAA and cortisol. In Experiment 2, a 2 × 2 factorial design was used to determine the effect of teeth clipping and time spent out of the farrowing crate post-clipping on skin temperature. Piglets from 60 litters had their teeth clipped (CLIP) or left intact (INT) and were returned to the farrowing crate immediately or after 1 min. Skin temperature was measured after piglets were returned to the farrowing crate and after 10 min. In Experiment 1, CLIP and GRIND piglets had significantly lower skin temperatures than INT piglets; skin temperature was also significantly reduced in CLIP piglets in Experiment 2. Skin temperature did not differ between time-out groups. Plasma levels of CRP and SAA did not differ between treatments on day 1; however, concentrations of both proteins were significantly higher on day 29. CLIP pigs had significantly higher concentrations of CRP in comparison with GRIND pigs on day 29. Stress caused by teeth resection provoked a transient reduction in skin temperature. Furthermore, both resection methods caused infection and/or inflammation, but to a similar degree as that caused by leaving the teeth intact. These results indicate that the welfare of piglets is better in the short term if their teeth are left intact; however, if teeth resection is necessary grinding can be recommended in preference to clipping.
Study of Antimicrobial Activity of the Callus Tissue Salvia Pratensis L. (Lamiaceae) In Vitro

—In this paper, we studied the antimicrobial activity of callus tissue Salvia pratensis L. obtained in vitro against Escherichia coli and Staphylococcus aureus . The most effective sterilizing agents for disinfection of plant explants with their introduction into in vitro culture were selected. The composition of the nutrient medium for the cultivation of callus tissues of S. pratensis has been optimized. A comparative analysis for the antibacterial activity of plant extracts obtained from callus tissues and intact plants was carried out, and as a result it was found that extracts from callus tissue demonstrate the most pronounced antibacterial properties in concentrations of 100% and at a dilution of 1:10, in contrast to extracts from leaves and flowers of an intact plant.
A Proposal for a Benchmark Generator of Weakly Connected Directed Graphs

The previous studies on detection of communities on complex networks were focused on nondirected graphs, such as Neural Networks, social networks, social interrelations, the contagion of diseases, and bibliographies. However, there are also other problems whose modeling entails obtaining a weakly connected directed graph such as the student access to the university, the public transport networks, or trophic chains. Those cases deserve particularized study with an analysis and the resolution adjusted to them. Additionally, this is a challenge, since the existing algorithms in most of the cases were originally designed for non-directed graphs or symmetrical and regular graphs. Our proposal is a Benchmark Generator of Weakly Connected Directed Graphs whose properties can be defined by the end-users according to their necessities. The source code of the generators described in this article is available in GitHub under the GNU license.
Barriers to mental health care for disadvantaged adolescents: perspectives of service providers

ABSTRACT Objective: To identify barriers and enablers to professional mental health service utilisation for disadvantaged adolescents from the perspective of mental health service providers. Method: This study incorporated qualitative research methods with eight semi-structured, face-to-face interviews completed with purposively recruited government, non-government and school mental health service providers. The data were collected from May to September 2015 in Perth, Western Australia. Data analysis was undertaken using content analysis. Results: Service providers identified multiple barriers and facilitators for disadvantaged adolescents accessing and using mental health services including: (1) pathways to mental health services; (2) how services listen to and interact with adolescents; (3) service environment, including youth-friendly locations and atmosphere; and (4) government and organisational policies. Discussion: These results highlight the need to simplify pathways and provide support for adolescents in negotiating the mental health system. Schools can be utilised as an important gatekeeper to mental health services. Legislation associated with adolescent mental health appears to be a barrier for adolescents in seeking or continuing with care. Service should place more emphasis on building trusted relationships with clients and developing capacity to perform outreach. Strategies to enable services to work collaboratively may reduce the risk that adolescents experience multiple referrals within the system. To reduce the impact on individuals and long-term costs, the State Government needs to make early intervention a priority and modifying the current service delivery system to be more inclusive of adolescents’ perspectives. Video abstract Read the transcript Watch the video on Vimeo
A Complex Network Overlapping Community Detection Algorithm based on K-Cliques and Fitness Function

This paper presents an algorithm for detecting overlapping communities in complex networks. The algorithm draws on the idea of the clique as the core of the community, and proposes to treat the overlapping community as a collection of all k-cliques. The algorithm uses random nodes as the initial community, and each iteration selects the node with the maximum fitness value of the community neighbor. All k-cliques of the node are added to the community. During the process, nodes with negative fitness are removed. It then realizes the partition of network community structures and detects overlapping nodes. In many experiments of computer-generated networks and real-world networks, algorithms based on this idea have achieved good experimental results, which also illustrates the feasibility of this idea. Furthermore, the time efficiency and complexity of the algorithm is also acceptable. This algorithm also has better community discovery results.
Towards a comprehensive and dynamic conceptual framework to research and enact professional learning communities in the context of secondary education

ABSTRACT The past two decades research on teachers’ professional development focusses increasingly on professional learning communities (PLCs). Based on a literature search a conceptual framework was developed including a comprehensive PLC-concept. Comprehensive because it takes into account the complexity of the educational context. The PLC-concept outlining 11 characteristics, is embedded in a framework, containing five external factors that influence. The framework was evaluated by interviewing educational experts. The literature search and the interviews showed a great diversity of distinct PLC characteristics and a wide range of external influencing factors. Based on expert interviews, no essential elements appeared to be lacking in the framework, moreover no other perspectives on the framework were mentioned. An additional search on recent literature confirmed the constructed framework. As yet we concluded that the developed concept is sufficiently comprehensive and feasible for conducting research on PLCs.