Miracle-dz/UniFAR-FTUs huggingface dataset:
Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice

Cytochrome P‐450 (CYP)‐derived epoxyei‐cosatrienoic acids (EETs) possess potent anti‐inflammatory effects in vitro. However, the effect of increased CYP‐mediated EET biosynthesis and decreased soluble epoxide hydrolase (sEH, Ephx2)‐mediated EET hydrolysis on vascular inflammation in vivo has not been rigorously investigated. Consequently, we characterized acute vascular inflammatory responses to endotoxin in transgenic mice with endothelial expression of the human CYP2J2 and CYP2C8 epoxygenases and mice with targeted disruption of Ephx2. Compared to wild‐type controls, CYP2J2 transgenic, CYP2C8 transgenic, and Ephx2−/− mice each exhibited a significant attenuation of endotoxin‐induced activation of nuclear factor (NF)‐κB signaling, cellular adhesion molecule, chemokine and cytokine expression, and neutrophil infiltration in lung in vivo. Furthermore, attenuation of endotoxin‐induced NF‐κB activation and cellular adhesion molecule and chemokine expression was observed in primary pulmonary endothelial cells isolated from CYP2J2 and CYP2C8 transgenic mice. This attenuationwas inhibited bya putative EET receptor antagonist and CYP epoxygenase inhibitor, directly implicating CYP epoxygenase‐derived EETs with the observed anti‐inflammatory phenotype. Collectively, these data demonstrate that potentiation of the CYP epoxygenase pathway by either increased endothelial EET biosynthesis or globally decreased EET hydrolysis attenuates NF‐κB‐dependent vascular inflammatory responses in vivo and may serve as a viable anti‐inflammatory therapeutic strategy. —Deng, Y. , Edin, M. L. , Theken, K N. , Schuck, R N. , Flake, G. P., Kannon, M. A. , DeGraff, L. M. , Lih, F. B. , Foley, J., Bradbury, J. A. , Graves, J. P., Tomer, K. B. , Falck, J. R. , Zeldin, D. C. , Lee, C. R. Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice. FASEB J. 25, 703–713 (2011). www.fasebj. org
The Higher Accuracy Fourth-Order IADE Algorithm

˜This study develops the novel fourth-order iterative alternating decomposition explicit (IADE) method of Mitchell and Fairweather (IADEMF4) algorithm for the solution of the one-dimensional linear heat equation with Dirichlet boundary conditions. The higherorder finite difference scheme is developed by representing the spatial derivative in the heat equation with the fourth-order finite difference Crank-Nicolson approximation. This leads to the formation of pentadiagonal matrices in the systems of linear equations. The algorithm also employs the higher accuracy of the Mitchell and Fairweather variant. Despite the scheme’s higher computational complexity, experimental results show that it is not only capable of enhancing the accuracy of the original corresponding method of second-order (IADEMF2), but its solutions are also in very much agreement with the exact solutions. Besides, it is unconditionally stable and has proven to be convergent. The IADEMF4 is also found to be more accurate, more efficient, and has better rate of convergence than the benchmarked fourth-order classical iterative methods, namely, the Jacobi (JAC4), the Gauss-Seidel (GS4), and the successive over-relaxation (SOR4) methods.
Subbarrel domains in rat somatosensory (S1) cortex

We used cytochrome oxidase (CO) histochemistry in conjunction with other histological methods to investigate the histochemoarchitecture of barrel hollows in rat somatosensory cortex. We found that individual large barrels in the posteromedial barrel subfield encompass two or three discrete subbarrel domains. Detailed analysis revealed, further, that subbarrel domains are relatively consistent in size, each having average dimensions that approximate those of large barrels in mouse S1. Unexpectedly, subbarrel domains are organized into a few distinct, repeated patterns. The small barrels in rat anterolateral barrel subfield and all barrel hollows in mouse S1 appear to consist of single CO domains. Subbarrel domains revealed here by CO are columnar entities that correspond with cyto‐ and myeloarchitectonic inhomogeneities within the barrels and are enriched in thalamocortical axon terminals. The present findings together with existing data indicate that barrels in rat posteromedial barrel subfield are structurally and functionally heterogeneous. J. Comp. Neurol. 490:414–426, 2005. © 2005 Wiley‐Liss, Inc.
The optimal planning and dynamic operation of distributed generation method based on modified multiobjective optimization in power distribution system

This paper proposes an optimal method to plan and dynamically operate the distributed generation (DG) based on the modified nondominated sorting genetic algorithm II (NSGA-II). First, the uncertainty of load and DG (photovoltaic panels) output are considered. Daily summer load data and ideal photovoltaic (PV) panels output data are taken from a local electrical company in Albuquerque. To model the uncertainty of load, the daily load is randomized to each bus for every time interval (15 minutes), but with their sum equal to the daily load. The uncertainty of PV output is modeled by adding the cloudy index and frequency factor to the ideal PV output. Second, the placement of a DG is defined by voltage sensitivity analysis. The bus with high voltage sensitivity is considered to have priority for the installation of DG. To find the optimal daily operation of DG, a multiobjective problem is formulated that focuses on the minimization of a circuit's voltage deviations, active and reactive power losses. To solve the problem, the traditional NSGA II is modified by incorporating a fuzzy logic decision model. The fuzzy logic model selects an optimally compromised solution from the Pareto front by analyzing its weights of voltage deviations, active and reactive power losses. The selected solution includes the optimal outputs for every generator, synchronous compensator, capacitor bank, and PV panels for each time interval. Furthermore, to operate DG optimally and dynamically, the method's computation speed is crucial. To increase the modified NSGA II computation speed, the population initialization space is reduced and the population is selected and saved for the next generation based on load analysis and experiments. The method is tested on the IEEE 14 bus and a local residential circuit. The results on reducing the power losses, voltage deviations, and increasing the algorithm speed demonstrate the effectiveness of this method.
The benefits of monitoring routing protocols in live networks

This paper examines the benefits of deploying protocol centric network monitoring tools in IP and BGP/MPLS VPN networks. For the past three years, we have analysed the routing traffic from the following protocols: IS-IS, BGP and MP-BGP in three different autonomous systems. We explain how one can transform routing protocol packets into valid network management information. We demonstrate that protocol-centric monitoring tools allow unprecedented troubleshooting of routing issues, particularly in the case of BGP/MPLS VPN networks. We also outline that in the BGP/MPLS VPN AS, control plane traffic characteristics are very different from the internet control plane traffic. As such handling BGP/MPLS VPN control plane is a challenge for large service providers from the testing, monitoring and deployment point of views.
cis-active structural motifs involved in specific encapsidation of Moloney murine leukemia virus RNA

We have analyzed the roles of RNA structural motifs located in the 5' part of the Moloney murine leukemia virus (M-MuLV) encapsidation domain (Psi region) with regard to their effects on viral replication. Four putative stem-loop structures between the 5' splice donor site and the gag initiation codon have been examined: stem structure A, corresponding to M-MuLV viral nucleotides 211 to 224; stem-loop B, nucleotides 278 to 303; stem-loop C, nucleotides 310 to 352; and stem-loop D, nucleotides 355 to 374. By measuring infectivities, encapsidation and splicing efficiencies, and endogenous reverse transcription levels of motif A, B, C, and D deletion mutants, we identified mutations which affect replication at the encapsidation step. In particular, deletion of all four motifs in a single mutant eliminated encapsidation of viral RNA, while deletion of individual elements moderately reduced the encapsidation efficiencies. Through analysis of different deletion combinations, we found that deletion of the first two motifs (A plus B) reduced both encapsidation and reverse transcription efficiencies, while deletion of the 3' motifs (C plus D) eliminated encapsidation. Interestingly, the C and D motifs both contain a GACG loop sequence and are highly conserved among murine type C retroviruses. Our results indicate that M-MuLV motifs C and D are necessary for efficient encapsidation, and the presence of at least one of these two stem-loops is crucial to encapsidation and virus replication.
Delay-independent stability for a class of switched nonlinear systems with time-varying delays

In this paper, we study the stability for a class of switched nonlinear systems with multiple time-varying delays. Under milder constraints on system matrices and delays, a delay-independent stability criterion for the switched nonlinear system is established, which generalizes the main results by Liu [24] and Sun [25]. The method used in this paper is different from those usual methods developed for positive systems. Finally, we extend the main result to a class of time-varying switched nonlinear systems with unbounded delays.
Primal-Dual Interior Methods for Nonconvex Nonlinear Programming

This paper concerns large-scale general (nonconvex) nonlinear programming when first and second derivatives of the objective and constraint functions are available. A method is proposed that is based on finding an approximate solution of a sequence of unconstrained subproblems parameterized by a scalar parameter. The objective function of each unconstrained subproblem is an augmented penalty-barrier function that involves both primal and dual variables. Each subproblem is solved with a modified Newton method that generates search directions from a primal-dual system similar to that proposed for interior methods. The augmented penalty-barrier function may be interpreted as a merit function for values of the primal and dual variables. An inertia-controlling symmetric indefinite factorization is used to provide descent directions and directions of negative curvature for the augmented penalty-barrier merit function. A method suitable for large problems can be obtained by providing a version of this factorization that will treat large sparse indefinite systems.
American Cancer Society Guideline for the Early Detection of Cervical Neoplasia and Cancer

An update to the American Cancer Society (ACS) guideline regarding screening for the early detection of cervical neoplasia and cancer, based on recommendations from a formal review and recent workshop, is presented. The new screening recommendations address when to begin screening, when screening may be discontinued, whether to screen women who have had a hysterectomy, appropriate screening intervals, and new screening technologies, including liquid-based cytology and HPV DNA testing.
Antiprogestins Mediate Differential Effects on Glucocorticoid Receptor Remodeling of Chromatin Structure*

We examined the mechanism(s) by which the progesterone receptor (PR) is able to inhibit glucocorticoid receptor (GR) activation from the mouse mammary tumor virus (MMTV) promoter in vivo. Using specific hormone antagonists, we demonstrate that the PR complexed with an type II antiprogestin blocks glucocorticoid-induced activation of the MMTV promoter. However, when complexed with a type I antiprogestin the PR is unable to block glucocorticoid-induced activation. PR repression of GR activity results from the inhibition of the ability of the GR to remodel chromatin such that the antiprogestin-occupied/PR prevents the glucocorticoid induced assembly of a preinitiation complex at MMTV promoter. These experiments suggest that the specific chromatin organization of the MMTV promoter provides a mechanism for regulating cross-talk between the GR and PR in vivo.
Multi-hop wireless sensor network for remote monitoring of soil moisture

This paper presents a case study of wireless sensor network (WSN) system for remote soil moisture monitoring. The development of this system is driven by the needs for having realtime data collection and remote monitoring access capabilities which are lacking in the conventional soil monitoring techniques. Our proposed system is developed using commercially available IRIS motes based on the IEEE 802.15. 4 communications standard. The surrounding humidity, temperature and soil moisture of the crops are gathered and transmitted to a gateway for further analysis. The users will be notified when the soil moisture falls below the optimum level and they can also observe the changes in the data remotely. We validate the system and investigate its performance with respect to the types of network topology. The link quality, RSSI, network lifetime and number of packet drops are measured for both single-hop and multi-hop topologies. The finding shows that the multi-hop network offers wider area coverage with strong link quality at the expense of higher energy consumption compared to the single-hop network.
Treatment of leachate by using sequencing batch reactor (SBR)

Rapid urbanization and industrialization changed the characteristics of solid waste generated and leads to severe environmental problems. Solid waste disposed of in landfills will go through several stages of decomposition, will eventually result in the liquid at the bottom of the landfill leachate. Now days, according to the increasingly restrictive limits for wastewater discharge, complicated and costly treatment facilities are imposed. This research will examined the performance of Sequencing Batch Reactor (SBR) on removal of suspended solid (SS), turbidity, chemical oxygen demand (COD), ammonia-nitrogen, total nitrogen (TN), and total phosphorus (TP). The variables are reaction time in differences condition (anaerobic, anoxic, and aerobic). Then, follow by combination of difference steps consisting anaerobic/ anoxic/ aerobic in order to achieve maximum removal. The characteristic of Pasir Gudang, Johor’s landfill leachate is pH, turbidity, BOD, COD, suspended solid, colour, ammonia nitrogen, total phosphorous, total nitrogen, total organic carbon, tannin and lignin, and sulfate, 8.06, 841 NTU, 2107 mg/L, 6000 mg/L, 576 mg/L, 1050 ADMI, 50.638 mg/L, 24 mg/L, 700 mg/L, 185 mg/L, 17 mg/L, 1005 mg/L. The expected outcomes parameter of suspended solid (SS), turbidity, chemical oxygen demand (COD), ammonia-nitrogen, total nitrogen (TN), and total phosphorus (TP) from leachate which is 80%, 80%, 90%, 76%,80%, and 70% respectively.
Assembly and Function of the Cytochrome cbb Oxidase Subunits in Bradyrhizobium japonicum(*)

The Bradyrhizobium japonicum cbb-type cytochrome oxidase, which supports microaerobic respiration, is a multisubunit enzyme encoded by the genes of the fixNOQP operon. We investigated the contribution of the individual subunits to function and assembly of the membrane-bound complex. In-frame deletion mutants of fixN, fixO, and fixQ, and an insertion mutant of fixP were constructed. All mutants, except the fixQ mutant, showed clearly altered absorption difference spectra of their membranes and decreased oxidase activities, and they were unable to fix nitrogen symbiotically. The presence of the individual subunits was assayed by Western blot analysis, using subunit-specific antibodies, and by heme staining of the c-type cytochromes FixO and FixP. These analyses led to the following conclusions: (i) FixN and FixO are necessary for assembly of the multimeric oxidase, (ii) FixN and FixO assemble independently of FixP, and (iii) FixQ is not required for complex formation and, therefore, does not seem to be an essential subunit. The possible oxidase biogenesis pathway involves the formation of a primary core complex consisting of FixN and FixO, which allows the subsequent association with FixP to form the complete enzyme.
Kinoform design with an optimal-rotation-angle method.

Kinoforms (i.e., computer-generated phase holograms) are designed with a new algorithm, the optimalrotation- angle method, in the paraxial domain. This is a direct Fourier method (i.e., no inverse transform is performed) in which the height of the kinoform relief in each discrete point is chosen so that the diffraction efficiency is increased. The optimal-rotation-angle algorithm has a straightforward geometrical interpretation. It yields excellent results close to, or better than, those obtained with other state-of-the-art methods. The optimal-rotation-angle algorithm can easily be modified to take different restraints into account; as an example, phase-swing-restricted kinoforms, which distribute the light into a number of equally bright spots (so called fan-outs), were designed. The phase-swing restriction lowers the efficiency, but the uniformity can still be made almost perfect.
Survey of the year 2000 commercial optical biosensor literature.

We have compiled a comprehensive list of the articles published in the year 2000 that describe work employing commercial optical biosensors. Selected reviews of interest for the general biosensor user are highlighted. Emerging applications in areas of drug discovery, clinical support, food and environment monitoring, and cell membrane biology are emphasized. In addition, the experimental design and data processing steps necessary to achieve high-quality biosensor data are described and examples of well-performed kinetic analysis are provided.
Core histone hyperacetylation co‐maps with generalized DNase I sensitivity in the chicken beta‐globin chromosomal domain.

The distribution of core histone acetylation across the chicken beta‐globin locus has been mapped in 15 day chicken embryo erythrocytes by immunoprecipitation of mononucleosomes with an antibody recognizing acetylated histones, followed by hybridization probing at several points in the locus. A continuum of acetylation was observed, covering both genes and intergenic regions. Using the same probes, the generalized sensitivity to DNase I was mapped by monitoring the disappearance of intact genomic restriction fragments from Southern transfers. Close correspondence between the 33 kb of sensitive chromatin and the extent of acetylation indicates that one role of the modification could be the generation and/or maintenance of the open conformation. The precision of acetylation mapping makes it a possible approach to the definition of chromosomal domain boundaries.
Lentiviral Hematopoietic Stem Cell Gene Therapy in Patients With Wiskott-Aldrich Syndrome

A Aiuti, L Biasco, S Scaramuzza. Science. 2013;341(6148):1233151 The goal of this study was to develop a clinical protocol for Wiskott-Aldrich syndrome (WAS) by using a lentiviral vector (LV) to introduce a functional WAS gene into hematopoietic stem and progenitor cells (HSPCs) for autologous transplantation. Three patients who had WAS with a severe clinical condition or severe mutation/absent WAS protein expression without a suitable matched donor for allogeneic transplant or ineligible for HSPC transplantation were enrolled. Patients were pretreated with a reduced-intensity myeloablative regimen. Afterward, they received autologous HSPCs that were transduced …
Mitochondrial network energetics in the heart

At the core of eukaryotic aerobic life, mitochondrial function like ‘hubs’ in the web of energetic and redox processes in cells. In the heart, these networks—extending beyond the complex connectivity of biochemical circuit diagrams and apparent morphology—exhibit collective dynamics spanning several spatiotemporal levels of organization, from the cell, to the tissue, and the organ. The network function of mitochondria, i.e., mitochondrial network energetics, represents an advantageous behavior. Its coordinated action, under normal physiology, provides robustness despite failure in a few nodes, and improves energy supply toward a swiftly changing demand. Extensive diffuse loops, encompassing mitochondrial–cytoplasmic reaction/transport networks, control and regulate energy supply and demand in the heart. Under severe energy crises, the network behavior of mitochondria and associated glycolytic and other metabolic networks collapse, thereby triggering fatal arrhythmias. WIREs Syst Biol Med 2012, 4:599–613. doi: 10.1002/wsbm.1188
Functional and histological studies of the vagus nerve and its branches to the heart, lungs and abdominal viscera in the cat

Although a number of studies has been made on the distribution of nerve fibres in the vagus and its branches (Ranson, Foley & Alpert, 1933; Heinbecker & O'Leary, 1933; DuBois & Foley, 1936; Foley & DuBois, 1937; Daly & Evans, 1953; Evans & Murray, 1954a), few observations have been reported of differential sensory and motor counts in the nerve. DuBois & Foley (1936) determined the proportion of sensory and motor fibres in some of the cervical branches of the vagus. In a later paper (Foley & DuBois, 1937) they demonstrated that in the vagus nerve above the nodose ganglion in the cat the sensory group constituted 65-80% of the total fibre count. Daly & Evans (1953) studied the effects of electrical stimulation of the caudal cut end of the cervical vagus nerve in the cat after chronic division at various levels. Their results indicated that the motor functions of the vagus to the heart and bronchial musculature are conveyed by myelinated fibres, 2-4,u in size, and also by non-myelinated fibres. Large numbers of myelinated fibres of all sizes (1-14 ,u) and non-myelinated fibres in the thoracic vagus and its cardiac and bronchial branches survive chronic intracranial vagotomy and are therefore afferent in function. A similar study was made in the rabbit by Evans & Murray (1954 a). In this species, the motor functions of the vagus to the lungs are served almost entirely by non-myelinated fibres; afferent fibres are composed of myelinated fibres of all sizes (1-12,) and by a few non-myelinated fibres. These authors demonstrated further that the vagus nerves as they enter
Relevance of Hyperglycemia on the Timing of Functional Loss of Allogeneic Islet Transplants: Implication for Mouse Model

Background. The role of recipient hyperglycemia on timing of allograft survival is unknown. In this study, we investigated if and how variation in recipient glycemia affects the ability to achieve and maintain normoglycemia after transplant of C57BL/6 islets into diabetic BALB/c mice. Methods and Results. 85 diabetic BALB/c mice with non-fasting glycaemia ranging between 275 and 600 mg/dL were transplanted with 400 C57BL/6 islets. The time of rejection inversely correlated with the pre-transplant blood glucose concentration (P=0.004). All the 13 mice with normoglycemia beyond 50 days had pretransplant glycemia <450 mg/dL and the presence of autologous beta cell function was demonstrated in 8 (>100 days function) by the persistence of normoglycemia after allograft removal. The presence of immunosuppression (rapamycin plus FK506 plus anti–IL-2Ra chain mAbs, n=31; rapamycin plus IL-10; n=29) removed the influence of pretransplant hyperglycemia but after treatment withdrawn the timing and the probability of graft loss correlate with the pretransplant hyperglycemia. Pretransplant glycemia was inversely correlated with HOMA-B and serum insulin showing that a significant residual beta cell mass was present in mice with glycemia <450 mg/dL. Conclusion. This study demonstrates that the timing of functional loss of islets allotransplantation depends on the degree of recipient hyperglycemia. This potential bias should be kept in count in experimental results and a threshold that excludes moderate diabetes should be used in defining recipient’s eligibility.