Miracle-dz/UniFAR-FTUs huggingface dataset:
Classification of music into moods using musical features

Usually, music is generally classified on the basis of its genre which indicates its musical style or musical form based on some sort of shared history. On the contrary, this paper aims to classify a given track into a mood such as happy, sad, peaceful and angry rather than based on its genre because more often than not, the listener prefers to hear songs similar to each other both in terms of the mood it evokes in the listener and otherwise. This is done by using the valence and arousal values that came along with the 1000 song database that we are using, as well as extraction of several musical features such as tempo, energy, pitch, etc. Using a combination of all these features, we use 4 different classification algorithms namely — Support Vector Machine (SVM), Naive Bayes, Linear Discriminant Analysis and Decision Trees to classify the music into the aforementioned moods and the accuracy achieved with these classifiers is 59.8%, 75.4%,78.5% and 71.4% respectively.
Roflumilast: Who Is Using It and How It Affects Health Care Resource Utilization and Costs.

OBJECTIVE Compare baseline characteristics, health care resource utilization (HCRU), and associated costs of COPD patients treated with add-on roflumilast with those of other combination medications. DESIGN Retrospective cohort study. METHODOLOGY Patients aged 40 years with a diagnosis of chronic obstructive pulmonary disease (COPD) between March 1, 2011, and Nov. 30, 2012, were identified from the HealthCore Integrated Research Database and classified as roflumilast or nonroflumilast combination-therapy cohorts. Baseline characteristics were compared for all patients. HCRU and costs were compared between matched (M) roflumilast and nonroflumilast cohorts, using propensity score as a partial balancing score and then complementing the score with exact matching on specifically important variables. Generalized linear model and Poisson regression were used to estimate the adjusted differences in total costs and hospitalization rates, respectively, between the 2 matched cohorts. RESULTS A total of 695 roflumilast and 30,542 nonroflumilast combination therapy users were identified. At baseline, the roflumilast cohort had more complex COPD and a higher number of severe and moderate COPD exacerbations relative to the nonroflumilast cohort. After matching, the roflumilast (M) and nonroflumilast (M) cohorts (n = 328 in each) had similar mean age, gender distribution, and follow-up time. The roflumilast (M) cohort had significantly higher pharmacy-related, per-patient, per-month (PPPM) costs (P < .001) and similar total cost (P = .90). After adjusting for confounding variables, no difference was observed between the 2 cohorts in total costs (P = .86) and number of hospitalizations (P = .65). CONCLUSION Findings suggest that patients in the roflumilast cohort, relative to the nonroflumilast cohort, were more severely ill in the real-world setting. Despite higher pharmacy costs, the total cost for the roflumilast cohort was statistically similar to the nonroflumilast cohort. Future studies with longer follow-up are needed to evaluate the long-term economic impact of roflumilast use.
Identification and Integrative Analysis of 28 Novel Genes Specifically Expressed and Developmentally Regulated in Murine Spermatogenic Cells*

Mammalian spermatogenesis is a highly ordered process that occurs in mitotic, meiotic, and postmeiotic phases. The unique mechanisms responsible for this tightly regulated developmental process suggest the presence of an intrinsic genetic program composed of spermatogenic cell-specific genes. In this study, we analyzed the mouse round spermatid UniGene library currently containing 2124 gene-oriented transcript clusters, predicting that 467 of them are testis-specific genes, and systematically identified 28 novel genes with evident testis-specific expression by in silico and in vitro approaches. We analyzed these genes by Northern blot hybridization and cDNA cloning, demonstrating the presence of additional transcript sequences in five genes and multiple transcript isoforms in six genes. Genomic analysis revealed lack of human orthologues for 10 genes, implying a relationship between these genes and male reproduction unique to mouse. We found that all of the novel genes are expressed in developmentally regulated and stage-specific patterns, suggesting that they are primary regulators of male germ cell development. Using computational bioinformatics tools, we found that 20 gene products are potentially involved in various processes during spermatogenesis or fertilization. Taken together, we predict that over 20% of the genes from the round spermatid library are testis-specific, have discovered the 28 authentic, novel genes with probable spermatogenic cell-specific expression by the integrative approach, and provide new and thorough information about the novel genes by various in vitro and in silico analyses. Thus, the study establishes on a comprehensive scale a new basis for studies to uncover molecular mechanisms underlying the reproductive process.
Genetic Diversity and Antibiotic Resistance of Escherichia coli Isolates from Different Leafy Green Production Systems.

Foodborne disease outbreaks linked to contaminated irrigation water and fresh produce are a public health concern. The presence of Escherichia coli isolates from irrigation water and leafy green vegetables in different food production systems (large commercial farms, small-scale farms, and homestead gardens) was investigated. The prevalence of antibiotic resistance and virulence in these isolates was further assessed, and links between water source and irrigated crops were identified using antimicrobial and genotypic analyses. Presumptive E. coli isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectroscopy, and identities were confirmed by PCR using the uidA gene. Antimicrobial susceptibility was evaluated with the Kirby Bauer disk diffusion test; the presence of virulence genes was determined with enterobacterial repetitive intergenic consensus PCR assays. Of the 130 E. coli isolates from water (n =60) and leafy green vegetables (n =70), 19 (14.6%) were resistant to one antibiotic (tetracycline) and 92 (70.7%) were resistant to various antibiotics (including ampicillin, cefoxitin, and nalidixic acid). All E. coli isolates were susceptible to ceftriaxone and gentamicin. The virulence gene stx2 was detected in E. coli isolates from irrigation water (8 [13.3%] of 60 isolates) and cabbages (3 [7.5%] of 40), but the virulence genes eae and stx1 were not detected in any tested isolates from irrigation water and fresh produce samples. The prevalence of multidrug-resistant E. coli was lower in isolates from GLOBALG.A. P.-certified farms than in isolates from noncertified commercial and small-scale farms and homestead gardens. A link between the E. coli isolates from irrigation water sources and leafy green vegetables was established with phenotypic (antimicrobial) and genotypic (DNA fingerprinting) analyses. However, a link between virulence genes and the prevalence of antimicrobial resistance could not be established.
Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen‐thawed boar semen

The main aim of this work was to evaluate how supplementing freezing media with reduced glutathione (GSH) affected the ‘in vivo’ fertilizing ability of boar semen subjected to cryopreservation procedures. With this purpose, 12 ejaculates coming from 12 boars were cryopreserved in the presence or absence of 2 mm GSH, whereas the same number of extended ejaculates coming from the same boars was used as negative/farm controls. Eight different sperm parameters (levels of free‐cysteine residues in sperm nucleoproteins, DNA fragmentation, sperm viability, acrosome‐membrane integrity, intracellular peroxide and superoxide levels, and total and progressive sperm motility) were evaluated before freezing and after 30 and 240 min of thawing. In addition, a total of 180 multiparous sows were used in the field fertility trials, the females being randomly divided into three groups and inseminated with extended, frozen‐thawed control or frozen‐thawed semen supplemented with 2 mm GSH. The presence of GSH in the freezing media significantly (p < 0.05) increased farrowing rates and the number of total born piglets and alive born piglets, and partially counteracted the cryopreservation‐induced damages inflicted on frozen‐thawed spermatozoa. We can thus conclude that supplementing freezing media with 2 mm GSH greatly improves boar sperm cryopreservation technology, as it significantly improves the fertilizing ability of frozen‐thawed spermatozoa.
Robot assisted localization of sensor networks guided by rigidity

In this paper, we propose a robot assisted localization guided by rigidity checking. For a network to be localizable, it must be rigid, which means given the existing edge constraints, there is only one location for each node in it. Many networks, however, are not rigid. To localize these nonrigid network, a robot can be used to add additional distance measure between nodes so that the network becomes rigid and localizable. To facilitate the localization and reduce the travel cost of the robot, the rigid subregions of the network are first identified. The identification of rigid subregions provides a guide for the movement of robots. A brief introduction to rigidity theory is presented in this paper. Experiments that evaluate the performance of the proposed approach are presented. The experimental results prove that the proposed approach is sound and gives excellent results compared to previous approaches for localization.
The Dynamics of Subsidy Removal with Heterogeneous Firms ∗

We develop a dynamic general equilibrium model with heterogeneous firms and endogenous entry and exit to quantify the shortand long-term effects of eliminating operating, output, intermediate input, and interest rate subsidies. We consider the effects on welfare, production decisions, and especially measured TFP. By comparing the results to those from a model with a representative firm, we decompose TFP gains into those from reallocation of resources from lowto high-productivity firms and those from adjustment in firms’scales of operation and mixes of inputs. Taking the entire equilibrium path into account is important because steady-state analysis alone may imply that the welfare gains from subsidy removal are understated or even negative.
Widespread Occurrence and Lateral Transfer of the Cyanobactin Biosynthesis Gene Cluster in Cyanobacteria

ABSTRACT Cyanobactins are small cyclic peptides produced by cyanobacteria. Here we demonstrate the widespread but sporadic occurrence of the cyanobactin biosynthetic pathway. We detected a cyanobactin biosynthetic gene in 48 of the 132 strains included in this study. Our results suggest that cyanobactin biosynthetic genes have a complex evolutionary history in cyanobacteria punctuated by a series of ancient horizontal gene transfer events.
Sensor and Control Aspects of Biped Robot "JOHNNIE"

The biped robot "Johnnie" is designed to achieve a dynamically stable gait pattern, allowing for high walking velocities. Very accurate and fast sensors were developed for the machine. In particular the design of the 3D-orientation sensor and the 6-axes force-torque sensor are presented. The control scheme is based on the information from these sensors to deal with unstructured terrain and disturbances. Two different implementations are investigated: a computed torque approach and a trajectory control with adaptive trajectories. Walking speeds of 2.2 km/h have been achieved in experiments.
Electromagnetic and Thermal Analysis of Open-Circuit Air Cooled High-Speed Permanent Magnet Machines With Gramme Ring Windings

This paper investigates the electromagnetic and thermal performances of the open-circuit air cooled high-speed permanent magnet motor (HSPMM) with Gramme ring windings. Take a 36 kr/min, 75 kW HSPMM as an example, the electromagnetic losses, in particular the rotor eddy current loss and the stator iron loss of the motor are calculated. The influences of switching frequency and air gap length on these losses are studied. Then, a computational fluid dynamics (CFD) model is composed to calculate its coolant flow parameters and the temperature distribution. Based on the CFD results, a lumped-parameter thermal network (LPTN) is created and verified. Then, a 24 kr/min, 140 kW prototype with the same structure and cooling configuration is designed using the LPTN. Experiments show that the calculated results are with reasonable accuracy. Finally, a sensitivity analysis is applied to the 140 kW prototype based on the LPTN to disclose the influences of different parameters on the rotor permanent magnet temperature rise.
nucleotide methylation sRNP-guidedfor sRNP assembly but is critical for archaeal box C / D The coiled-coil domain of the Nop 56 / 58 core protein is dispensable

Archaeal box C/D sRNAs guide the methylation of specific nucleotides in archaeal ribosomal and tRNAs. Three Methanocaldococcus jannaschii sRNP core proteins (ribosomal protein L7, Nop56/58, and fibrillarin) bind the box C/D sRNAs to assemble the sRNP complex, and these core proteins are essential for nucleotide methylation. A distinguishing feature of the Nop56/58 core protein is the coiled-coil domain, established by a-helices 4 and 5, that facilitates Nop56/58 self-dimerization in vitro. The function of this coiled-coil domain has been assessed for box C/D sRNP assembly, sRNP structure, and sRNP-guided nucleotide methylation by mutating or deleting this protein domain. Protein pull-down experiments demonstrated that Nop56/ 58 self-dimerization and Nop56/58 dimerization with the core protein fibrillarin are mutually exclusive protein:protein interactions. Disruption of Nop56/58 homodimerization by alteration of specific amino acids or deletion of the entire coiledcoil domain had no obvious effect upon core protein binding and sRNP assembly. Site-directed mutation of the Nop56/58 homodimerization domain also had no apparent effect upon either box C/D RNPor C9/D9 RNP-guided nucleotide modification. However, deletion of this domain disrupted guided methylation from both RNP complexes. Nuclease probing of the sRNP assembled with Nop56/58 proteins mutated in the coiled-coil domain indicated that while functional complexes were assembled, box C/D and C9/D9 RNPs were altered in structure. Collectively, these experiments revealed that the selfdimerization of the Nop56/58 coiled-coil domain is not required for assembly of a functional sRNP, but the coiled-coil domain is important for the establishment of wild-type box C/D and C9/D9 RNP structure essential for nucleotide methylation.
Opposing roles for medial and central amygdala in the initiation of noradrenergic cell responses to a psychological stressor

Psychological stressors trigger the activation of medullary noradrenergic cells, an effect that has been shown to depend upon yet‐to‐be‐identified structures located higher in the brain. To test whether the amygdala is important in this regard, we examined the effects of amygdala lesions on noradrenergic cell responses to restraint, and also looked at whether any amygdala cells that respond to restraint project directly to the medulla. Ibotenic acid lesions of the medial amygdala completely abolished restraint‐induced Fos expression in A1 and A2 noradrenergic cells. In contrast, lesions of the central amygdala actually facilitated noradrenergic cell responses to restraint. Tracer deposits in the dorsomedial (but not ventrolateral) medulla retrogradely labelled many cells in the central nucleus of the amygdala, but none of these cells expressed Fos in response to restraint. These data suggest for the first time that the medial amygdala is critical to the activation of medullary noradrenergic cells by a psychological stressor whereas the central nucleus exerts an opposing, inhibitory influence upon noradrenergic cell recruitment. The initiation of noradrenergic cell responses by the medial amygdala does not involve a direct projection to the medulla. Accordingly, a relay through some other structure, such as the hypothalamic paraventricular nucleus, warrants careful consideration.
The Brain Stem in Psychosis

Summary The brain stems of 24 mental hospital patients and 10 control patients were examined. Ten mental hospital patients were either deluded or auditorily hallucinated in clear consciousness, and of this group 8 presented with a diagnosis of schizophrenic syndrome. The nature and significance of lesions found in the midline reticular nuclei and the trigeminal n. are discussed. The results, and the dearth of other published material on this subject, indicate the need for further work in this field.
A GPU based Transient Dedisersion Search Engine for CRAFT

The Commensal Realtime ASKAP Fast Transient Survey (CRAFT[5]) will search the ASKAP data stream for fast (< 5sec) transient events, associated with the most extreme conditions in the Universe. The CRAFT search will run in parallel with all normal observing on ASKAP, giving a tremendous advance in the transient parameter space which can be searched. ASKAP offers high sensitivity, high resolution, and continous observations of a significant portion of the sky. However, to search across the wide field of view and at the data rates which are being provided by ASKAP is extremely challenging. Nevertheless, as reported here, we are on track to achieve the goals as laid out in the Survey Science Proposal. Using GPUs as a simple highly parallel compute-engine we can monitor the full field of view with a 5σ sensitivity of ∼Jy for a millisecond event covering the astronomically significant range of DMs. After that trigger detection we can download the beamformer data-buffer and image the sky at full sensitivity and spatial resolution with an arbitary frequency and time resolution.
Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails

The bacterial Lsm protein, host factor I (Hfq), is an RNA chaperone involved in many types of RNA transactions such as replication and stability, control of small RNA activity and polyadenylation. In this latter case, Hfq stimulates poly(A) synthesis and binds poly(A) tails that it protects from exonucleolytic degradation. We show here, that there is a correlation between Hfq binding to the 3′ end of an RNA molecule and its ability to stimulate RNA elongation catalyzed by poly(A)polymerase I. In contrast, formation of the Hfq–RNA complex inhibits elongation of the RNA by polynucleotide phosphorylase. We demonstrate also that Hfq binding is not affected by the phosphorylation status of the RNA molecule and occurs equally well at terminal or internal stretches of poly(A).
A polarization independent single layer Frequency Selective Surface for quadruple band pass

In the propound work, a compact single layer Frequency Selective Surface has been designed that possess the characteristics of being polarization independent. This attribute of the structure gives same frequency response irrespective of the 0° polarization or 90° polarization of electromagnetic wave. In this paper, three designs have been proposed, the first two designs has affected from different polarization but in the last design we have achieved polarization independent FSS as of symmetrical behavior of the structure. The superstrate is incorporated of FR-4 substrate and a single sided PEC layer on it. In the third design, structure possesses quadruple pass band that is Wi-max (2.5 GHz) band, WLAN (5.5 GHz) band, and dual X-band (8.6 /10.9 GHz). CST Microwave Studio 2014 has been used to realize the frequency response via simulations.
Absolute criteria Histologic demonstration of the parasite Direct visualization of the parasite by fundoscopic examination Evidence of cystic lesions showing the scolex of the parasite on CT or MRI Major criteria

Sashank Prasad, Rob Roy MacGregor, Pablo Tebas, Lourdes B. Rodriguez, Javier A. Bustos, and A. Clinton White, Jr. Departments of Neurology and Infectious Diseases, University of Pennsylvania, Philadelphia; Infectious Disease Section, Department of Medicine, Baylor College of Medicine, Houston, Texas; Service of Internal Medicine and Infectious Diseases, Hospital Guillermo Almenara, Empresa de Seguros de Salud, and Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru
Rapamycin Blocks Sexual Development in Fission Yeast through Inhibition of the Cellular Function of an FKBP12 Homolog*

FKBP12 is a ubiquitous and a highly conserved prolyl isomerase that binds the immunosuppressive drugs FK506 and rapamycin. Members of the FKBP12 family have been implicated in many processes that include intracellular protein folding, transport, and assembly. In the budding yeast Saccharomyces cerevisiae and in human T cells, rapamycin forms a complex with FKBP12 that inhibits cell cycle progression by inhibition of the TOR kinases. We reported previously that rapamycin does not inhibit the vegetative growth of the fission yeast Schizosaccharomyces pombe; however, it specifically inhibits its sexual development. Here we show that disruption of the S. pombe FKBP12 homolog,fkh1 +, at its chromosomal locus results in a mating-deficient phenotype that is highly similar to that obtained by treatment of wild type cells with rapamycin. A screen forfkh1 mutants that can confer rapamycin resistance identified five amino acids in Fkh1 that are critical for the effect of rapamycin in S. pombe. All five amino acids are located in the putative rapamycin binding pocket. Together, our findings indicate that Fkh1 has an important role in sexual development and serves as the target for rapamycin action in S. pombe.
Increased UV‐induced SCEs but normal repair of DNA damage in p53‐deficient mouse cells

UV‐induced sister chromatid exchanges (SCEs) in p53‐deficient mouse cells were studied to obtain more evidence regarding the involvement of p53 protein in the DNA repair pathway as a checkpoint protein. After 5 J/m2 UV irradiation, mutant‐type homozygous cells for p53‐deficiency showed the same number of SCEs as the heterozygous and wild‐type homozygous cells. In the heterozygous and wild‐type homozygous cells, no further increase of SCEs was observed after 10 J/m2 UV irradiation. In contrast, in mutant‐type homozygous cells about twice as many SCEs were induced by 10 J/m2 UV as by 5 J/m2 UV. In mutant‐type homozygous cells, fractions of S‐phase cells decreased just after 10 J/m2 UV irradiation, but recovered to higher than control levels within a short time, while in heterozygous and wild‐type homozygous cells, the decrease in S‐phase cells was prolonged by more than 6 hr and no increase above control levels was observed. Although no difference in UV sensitivity and repair of UV‐induced DNA damage was found among the 3 genotypes, which were determined by the relative colony‐forming ability after UV irradiation and removal of thymine dimers and (6‐4) photoproducts from cellular DNA, our data strongly suggest an impaired checkpoint function in p53‐deficient cells when DNA is damaged.
Steroid‐Eluting Epicardial Leads in Pediatrics: Improved Epicardial Thresholds in the First Year

From 1986 to 1991, we evaluated the clinical use of three new epicardial lead designs incorporating a steroid‐eluting electrode. Medtronic models SP2114 (bipolar high profile), 10320 (bipolar low profile), 10295A/4965 (unipolar low profile) steroid epicardial (SE) leads were used on either atrium or ventricle for a total of 21 lead placements in 17 patients. Energy thresholds (T) were calculated and compared with our most recent 16 nonsteroid epicardial (NE) Medtronic model 4951 lead implants for which T was available. SE leads demonstrated no acute T rise and continued T improvements at 1 year of follow‐up. We conclude that epicardial application of SE lead technology offers a major improvement in pacing lead function and potential pacemaker longevity over NE leads in pediatric patients in whom endocardial pacing is precluded by size or anatomy.