Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of polypeptides of the carbon metabolism machinery on the two‐dimensional protein map of Saccharomyces cerevisiae. Location of 23 additional polypeptides

Using a modification of the basic two‐dimensional polyacrylamide gel electrophoresis technique, we have undertaken a systematic identification of the polypeptides of the protein map of Saccharomyces cerevisiae corresponding to components of the carbon metabolism machinery. To the previous location of nine glycolytic enzyme polypeptides on the yeast protein map we add the location of 23 polypeptides. Ten of them were identified as corresponding to cytoplasmic enzymes of the carbon metabolism machinery and 13 were characterized as mitochondrial proteins. The criteria used to establish the identification of these polypeptides spots include migration with purified proteins, immunodetection, overproduction by plasmid–carrying strains and physiological behaviour.
Investigating and modeling metadata use to support information architecture development in the statistical knowledge network

Metadata and an appropriate metadata model are non-trivial components of information architecture conceptualization and implementation, particularly when disparate and dispersed systems are integrated. Metadata availability can enhance retrieval processes, improve information organization and navigation, and support management of digital objects. To support these activities efficiently, metadata need to be modeled appropriately for the tasks. The authors' work focuses on how to understand and model metadata requirements to support the work of end users of an integrative statistical knowledge network (SKN). They report on a series of user studies. These studies provide an understanding of metadata elements necessary for a variety of user-oriented tasks, related business rules associated with the use of these elements, and their relationship to other perspectives on metadata model development. This work demonstrates the importance of the user perspective in this type of design activity and provides a set of strategies by which the results of user studies can be systematically utilized to support that design.
Development of an ultra wide band GCPW to SIW transition

In this paper, a novel ultra-wideband GCPW to SIW transition is developed. The transition integrates the coupling slot and impedance transformer into one tapered coupling slot to achieve an ultra wide bandwidth. The tapered coupling slot converts the arbitrary impedance of the SIW to the GCPW port impedance. The vias were strategically located to suppress unwanted modes across the transition as well. The fabricated transition demonstrated over 10 GHz bandwidth with less than 0.4 dB insertion loss at X-band. Theoretical and experimental results are presented and compared with previously designed transitions.
RoboJockey: real-time, simultaneous, and continuous creation of robot actions for everyone

We developed a RoboJockey (Robot Jockey) interface for coordinating robot actions, such as dancing, similar to a "disc jockey" or "video jockey" who selects and plays recorded music or video for an audience, in this case, robot's actions, and giving people a new entertainment experience with robots. The system enables a user to choreograph a robot to dance using a simple visual language. Every icon on the interface is circular and can be operated from all positions around the tabletop interface. Users can coordinate the mobile robot's actions with a combination of back, forward, and rotating movements, and the humanoid robot's actions with a combination of arm and leg movements. Every action is automatically performed to background music. We demonstrated RoboJockey at a Japanese domestic symposium, and confirmed that people enjoyed using the system and successfully created entertaining robot dances.
The role of 5‐aminolevulinic acid in enhancing surgery for high‐grade glioma, its current boundaries, and future perspectives: A systematic review

5‐Aminolevulinic acid (5‐ALA) has been approved as an intraoperative adjunct in glioma surgery in Europe, but not North America. A systematic review was conducted to assess the evidence regarding 5‐ALA as a surgical adjunct. The MEDLINE, EMBASE, and CENTRAL databases were searched, using terms relevant to “5‐ALA” and “high‐grade gliomas. ” Included studies were based on adults aged ≥18 years who underwent surgical resection/biopsy. No language or date limitations were used. Forty‐three studies (1830 patients) were identified. Thirty‐six were coordinated by European countries, 2 were in the United States, and none were in Canada. One was randomized, 28 were prospective, and 14 were retrospective. Twenty‐six studies assessed the utility of 5‐ALA as a diagnostic tool, 24 assessed its influence on the extent of resection (EOR), 9 assessed survival, and 22 reported adverse events. 5‐ALA had high sensitivity and positive predictive value, whereas its specificity increased with additional adjuncts. The EOR increased with 5‐ALA, but only progression‐free survival was significantly influenced. Reporting of adverse events was not systematic. The use of 5‐ALA improved tumor visualization and thus enabled a greater EOR and perhaps increased survival. However, additional adjuncts may be necessary for maximizing the specificity of resection and patient safety. Additional parameters, such as patient quality of life and health economic analyses, would be informative. Thus, additional systematic collection of prospective evidence may be necessary for the global incorporation of this potentially valuable surgical adjunct into routine practice. Cancer 2016;122:2469–78. © 2016 American Cancer Society.
A New Transmucous-Buccal Formulation of Acetaminophen for Acute Traumatic Pain: A Non-inferiority, Randomized, Double-Blind, Clinical Trial.

BACKGROUND Acetaminophen (APAP) consumption is large and sometimes excessive, and guidelines suggest to diminish the dosage prescription. In emergency situations of mild/moderate pain intravenous (iv) APAP is recommended, but the route of administration is invasive. OBJECTIVE To determine the efficacy of a new transmucous-buccal (B) pharmaceutical form of 125mg-APAP in patients. To confirm the findings obtained in 2 previous clinical trials in healthy volunteers. STUDY DESIGN A randomized, double-blind, non-inferiority, clinical trial (NCT01586143) was carried out from 03/05/2012 to 13/05/2013. SETTING The study took place in the Emergency Department of the University Hospital, Clermont-Fd, France. METHODS Forty-three patients were included and 40 analyzed. Patients were eligible if they had leg or arm traumatic pain of moderate intensity. Pain intensity was measured using a numerical scale (0 - 10) at regular times for 120 minutes and the main endpoint was at 30 minutes. The hypothesis of non-inferiority was formulated from previous works with healthy volunteers. After pain assessment, patients received at baseline 1 g-iv-APAP or saline and concomitantly, 125 mg APAP in 1 mL hydroalcoholic solution (HAS) or placebo (HAS only) was applied in the left mucogingival sulcus. Non-inferiority of the primary outcome was assessed by one-sided 2 group t-test of equivalence in means with equal variances with a non-inferiority limit difference of 1. Other tests were two-sided, with a type I error set at α = 0.05. RESULTS Intention-to-treat analysis shows that pain intensity of B-APAP and iv-APAP groups were not significantly different at t30 minutes (3 ± 1.3 vs 2.7 ± 1.2, P = 0.23, one-sided Student t-test), and at any other times for 120 minutes. The difference of pain intensity between groups was 0.30 with 2-sided IC90% = [-0.38 - 0.98], not including the non-inferiority margin ( ∆ = 1). Time to exhibit a statistical significance in pain relief from baseline was reached at t10 for B-APAP (P = 0.03) and iv-APAP (P < 0.001). Patients preferred the buccal rather than the iv route of administration. LIMITATIONS Small population study with limited doses. CONCLUSIONS For acute traumatic pain of moderate intensity, B-APAP has a non-inferior analgesic effect compared to iv-APAP for 2 hours. Such a pharmaceutical form would be useful in emergency situations and breakthrough moderate pain episodes. It would diminish APAP consumption per dosage unit, limit the risk of adverse events and toxicity, and adhere to actual guidelines of APAP prescription. It must be now studied in a larger population and with repeated doses.
The uracil permease of Schizosaccharomyces pombe: A representative of a family of 10 transmembrane helix transporter proteins of yeasts

The uracil permease gene of Schizosaccharomyces pombe was cloned and sequenced. The deduced protein sequence shares strong similarities with five open reading frames from Saccharomyces cerevisiae, namely the uracil permease encoded by the FUR4 gene, the allantoin permease encoded by DAL4, a putative uridine permease (YBL042C) and two unknown ORFs YOR071c and YLR237w.
Signi fi cance of off-pump coronary artery bypass grafting compared with percutaneous coronary intervention : a propensity score analysis

OBJECTIVE: Although there have been several studies that compared the efficacy of percutaneous coronary intervention (PCI) and coronary artery bypass grafting (CABG), the impact of off-pump CABG (OPCAB) has not been well elucidated. The objective of the present study was to compare the outcomes after PCI, on-pump CABG (ONCAB), and OPCAB in patients with multivessel and/or left main disease. METHODS: Among the 9877 patients undergoing first PCI using bare-metal stents or CABG who were enrolled in the CREDO-Kyoto Registry, 6327 patients with multivessel and/or left main disease were enrolled into the present study (67.9 ± 9.8 years old). Among them, 3877 patients received PCI, 1388 ONCAB, and 1069 OPCAB. Median follow-up was 3.5 years. RESULTS: Comparing PCI with all CABG (ONCAB and OPCAB), propensity-score-adjusted all-cause mortality after PCI was higher than that CABG (hazard ratio (95% confidence interval): 1.37 (1.15–1.63), p < 0.01). The incidence of stroke was lower after PCI than that after CABG (0.75 (0.59–0.96), p = 0.02). CABG was associated with better survival outcomes than PCI in the elderly (interaction p = 0.04). Comparing OPCAB with PCI or ONCAB, propensity-score-adjusted all-cause mortality after PCI was higher than that after OPCAB (1.50 (1.20–1.86), p < 0.01). Adjusted mortality was similar between ONCAB and OPCAB (1.18 (0.93–1.51), p = 0.33). The incidence of stroke after OPCAB was similar to that after PCI (0.98 (0.71–1.34), p > 0.99), but incidence of stroke after ONCAB was higher than that after OPCAB (1.59 (1.16–2.18), p < 0.01). CONCLUSIONS: In patients with multivessel and/or left main disease, CABG, particularly OPCAB, is associated with better survival outcomes than PCI using bare-metal stents. Survival outcomes are similar between ONCAB and OPCAB.
A randomized study of buffy coat platelets in platelet additive solution stored 1–5 versus 6–7 days prior to prophylactic transfusion of allogeneic haematopoietic progenitor cell transplant recipients

Background and Objective Storage of platelets > 5 days provides improved availability, logistical management and decreased outdating. Promising results on in vitro parameters and on in vivo post‐transfusion recovery and survival of autologous platelets in healthy volunteers have earlier been shown. To provide additional verification, randomized patient transfusion studies are needed.
Comparison of fluconazole and amphotericin B for treatment of experimental Candida albicans endocarditis in rabbits

Amphotericin B (AmB) and fluconazole, administered intraperitoneally for 7 days, were compared in a rabbit model for Candida albicans endocarditis. When given early, AmB was more effective than fluconazole for reducing CFU counts in vegetations (P < 0.01) and kidneys. Forty-eight hours after the last dose, AmB was still detected in all vegetations whereas fluconazole was detected in only one case.
Common-Duty-Ratio Control of Input-Series Output-Parallel Connected Phase-shift Full-Bridge DC–DC Converter Modules

Input-series output-parallel (ISOP)-connected converters allow the use of low-voltage and low-power converter modules for high input-voltage and high-power applications. Further, the use of high-frequency, low-voltage MOSFETs, which are optimized for very low on-resistance, is enabled, resulting in lower conduction losses and higher power density. In this paper, a common-duty-ratio control scheme is proposed for an ISOP converter consisting of multiple phase-shift full-bridge (PS-FB) converter modules. The proposed control method achieves stable sharing of the input voltage and load current by applying a common duty ratio to all converter modules, without a dedicated input-voltage sharing controller. The control method is analyzed by using both a small-signal averaged model and a steady-state dc model of the ISOP converter, and it is concluded that the equal sharing of input voltage and load current among converter modules can be achieved through reducing the mismatches in various module parameters, which is practically achievable. The stability and performance of the control scheme are verified by Saber simulation and a 500 W experimental prototype consisting of two PS-FB converter modules.
Ascorbic Acid Enhances Iron-induced Ferritin Translation in Human Leukemia and Hepatoma Cells (*)

Ascorbate is an important cofactor in many cellular metabolic reactions and is intimately linked to iron homeostasis. Continuously cultured cells are ascorbate deficient due to the lability of the vitamin in solution and to the fact that daily supplementation of media with ascorbate is unusual. We found that ascorbate repletion alone did not alter ferritin synthesis. However, ascorbate-replete human hepatoma cells, Hep3B and HepG2, as well as K562 human leukemia cells achieved a substantially higher cellular ferritin content in response to a challenge with iron than did their ascorbate-deficient counterparts grown under standard culture conditions. Most of the elevation in ferritin content was due to an increase in de novo ferritin synthesis of greater than 50-fold, as shown by in vivo labeling with [S]methionine and immunoprecipitation. RNA-blot analysis showed only minor changes in steady state levels of ferritin mRNA, suggesting that ascorbate enhances iron-induced ferritin synthesis primarily by post-transcriptional events. Transient gene expression experiments using chloramphenicol acetyltransferase reporter gene constructs showed that the ascorbate effect on ferritin translation is not mediated through the stem-loop near the translational start site that transduces ferritin synthesis in response to cytokines. The data suggest that ascorbate possibly modifies the action of the iron-responsive element on ferritin translation, although more precise structure-function studies are needed to clarify this issue. These data demonstrate a novel role of ascorbate as a signaling molecule in post-transcriptional gene regulation. The mechanism by which ascorbate modulates cellular iron metabolism is complex and requires additional detailed investigation.
Single motor unit variability with threshold stimulation in patients with amyotrophic lateral sclerosis and normal subjects

Repetitive nerve stimulation often shows responses with an abnormal decrement in patients with amyotrophic lateral sclerosis (ALS), suggesting instability of the neuromuscular junction; however, the pathophysiology and response characteristics of this instability are not clear. We evaluated response variability of 47 single motor units from 16 patients with ALS and 51 units from 10 normal subjects, acquired by delivering threshold stimuli sporadically at 0.5 HZ or less. In addition, in 46 other different single motor units obtained from 21 patients with ALS, variability was studied at both 1‐ and 3‐HZ stimulation rates. Motor units from patients with ALS were significantly more variable than those from normal subjects, even when their larger amplitude was accounted for. This increased variability was not rate dependent. Response variability is a critical measure in the statistical method of motor unit number estimation and is attributed to variability in the number of units activated; the fact that variability of single motor units varies with disease state may be a potentially confounding factor in the application of the technique. Muscle Nerve, 2004
Differential capacity of wild type promoter elements for binding and trans-activation by retinoic acid and thyroid hormone receptors.

Retinoic acid receptor (RAR) and thyroid hormone receptor (T3R) are structurally similar and can bind as homodimers or T3R-RAR heterodimers to a single synthetic DNA response element. The interaction of these two types of receptors with wild type elements, however, has not been systematically investigated. Promoter elements from genes regulated by retinoic acid (RA) or thyroid hormone (T3) were tested for response to T3 and RA in transient transfections in both JEG and COS cells. The elements were classified as primarily responsive to RA or to T3 or responsive to both ligands. Binding of highly purified RAR alpha and T3R alpha to the various elements was assessed using the gel shift assay. Those elements predominantly responsive to one ligand showed preferential binding to the appropriate receptor. A series of point mutations were introduced into the rat GH T3 response element to further define sequence requirements for response to both RA and T3. Down-mutations in any of the three hexamers (previously demonstrated to be required for full response to T3 and full binding of T3R) also decreased RA induction and RAR binding. However, only one of two sets of up-mutations for T3 response also increased RA induction, demonstrating differences in hexamer preference between RAR and T3R. Variation in spacing of the three hexamers did not influence RA vs. T3 induction or RAR vs. T3R binding according to the predictions of a simple hexamer spacing model. There was a strong correlation between the extent of T3R dimer binding and strength of T3 induction for a subset of elements studied in JEG cells (r = 0.97, P < 0.01) and a weaker but significant correlation in COS cells (r = 0.65, P < 0.05)). In contrast, RAR dimer binding by the wild type elements did not quantitatively correlate with RA induction in either JEG (r = 0.13, P > 0.05) or COS cells (r = 0.21, P > 0.05). These results suggests that RAR interacts with a heterodimer partner(s) which influences binding site specificity, whereas T3R heterodimer partner(s) is less likely to alter binding site recognition. The observed difference in COS and JEG cells as well as the weak T3R binding-function relationship of the malic enzyme element, however, suggest that the influence of T3R heterodimer partner(s) on binding site specificity is likely to vary with cell type and the specific element tested.
Patent Licensing

While the title of this paper is a very general one, I will spend most of my time discussing the licensing of patents which cover inventions made at educational and scientific research institutions. I will draw heavily on my personal experiences gained during the last 17 years with the invention administration programs of Research Corporation. It should be noted, however, that these perspectives will admirably illustrate the general principles involved in developing and carrying out any patent licensing program. Inventions and patents covering inventions may be brought into public use by several means. The owner of an invention or patent may, himself, undertake its further development and marketing. He may do this jointly in partnership with other individuals, or he may outright sell or assign the invention, know-how associated with it, or corresponding patents to others for commercialization. By far the most widely-used method of developing commercial interest in university inventions is through licensing them to other individuals or industrial organizations with the necessary entrepreneurial, financial, managerial, technical, and marketing capabilities. This method is particularly applicable to patents covering university inventions, and is the primary method used by Research Corporation. Usually, licensing of inventions and the patents covering them brings the greatest overall financial return to the patent owner at minimum risk and with least involvement in areas where needed expertise may be weak or missing. Little or no capital investment is needed, business and managerial problems are minimized, market development is rapid, and a wide distribution of final products is accomplished in minimum time. However, like the proverbial rabbit stew where the first cookbook instruction is to first catch the rabbit, one must first at least file an application for a patent before patent licensing can begin. Let me now digress to discuss briefly the evaluation and patenting of inventions, necessary preliminary steps to obtaining issued patents.
Generalization of the Barnes-Hut algorithm for the Helmholtz equation in three dimensions

The Barnes-Hut algorithm originally developed for O(N log N) solution of the iV-body problem with the Laplace kernel is generalized to the case of the scalar Helmholtz kernel in three dimensions. Analogous to the center-of-charge concept used in the static algorithm, a center-of-radiation (CoR) is associated with each box of the hierarchically partitioned space enclosing the sources of interest. The algorithm is applicable to the acceleration of electromagnetic interactions between sources confined to electrically small volumes. The method may be used in conjunction with the high-frequency fast multipole method (FMM) to eliminate its low-frequency breakdown.
Indeterminate results of the second-generation hepatitis C virus (HCV) recombinant immunoblot assay: significance of high-level c22-3 reactivity and influence of HCV genotypes

A second-generation recombinant immunoblot assay (RIBA 2.0) is used in the United States to confirm infection with hepatitis C virus (HCV) in samples that are anti-HCV (enzyme immunoassay) positive. In some cases, indeterminate results of RIBA 2.0, which are defined as reactivity to a single antigen species or reactivity limited to two proteins derived from the same coding region of the HCV genome, are encountered. This study was performed to establish the significance of indeterminate RIBA 2.0 results in relation to HCV RNA detection, high positivity for the c22-3 band, and the HCV genotype as determined by direct DNA sequencing. Ninety-six samples with indeterminate RIBA 2.0 results were studied. HCV RNA was detected in 21 of 34 (62%) samples with high reactivity to c22-3 and in 8 of 62 (13%) samples with low reactivity to c22-3. The HCV genotype distribution in samples that were RIBA 2.0 indeterminate and HCV RNA positive was significantly different from that in samples of a control group with positive results for both the RIBA 2.0 and HCV PCR. These results suggest that highly positive c22-3 samples are likely to be associated with HCV viremia and that infection with less common HCV genotypes is more commonly associated with indeterminate RIBA 2.0 results.