Miracle-dz/UniFAR-FTUs huggingface dataset:
Catalytic Activities of α3β3γ Complexes of F1-ATPase with 1, 2, or 3 Incompetent Catalytic Sites*

In order to know how many functional catalytic sites are necessary for ATPase activity of F1-ATPase from a thermophilic Bacillus PS3, a new method of isolating homogeneous preparations of the α3β3γ complex with 1, 2, or 3 incompetent catalytic sites was developed. Ten glutamic acids (Glu·Tag) were linked to the C terminus of the catalytically incompetent β(E190Q) subunit. The Glu·Tag itself did not affect ATPase activity of the complexes. Two kinds of α3β3γ complexes, one containing β(wild-type) and the other Glu·Tag-linked β(E190Q), were mixed, urea-denatured, and dialyzed, and α3β3γ complexes were reconstituted. Each of the complexes containing a different number of Glu·Tag-linked β(E190Q) was separated by anion-exchange chromatography and analyzed. The results were as follows. 1) Normal steady-state ATPase activity requires three intact catalytic sites. 2) Chase-acceleration, a catalytic cooperativity, requires at least two intact catalytic sites. 3) Single-site catalysis can be mediated by a single intact catalytic site alone. Rescrambling of subunits between complexes could occur when the complex was aged under certain conditions, and this might be one of the reasons for previous contradictory results (Miwa, K., Ohtsubo, M., Denda, K., Hisabori, T., Date, T., and Yoshida, M. (1989) J. Biochem. (Tokyo) 106, 730-734).
Quantitative trait loci analysis for resistance against Turnip mosaic virus based on a doubled-haploid population in Chinese cabbage

Turnip mosaic virus (TuMV) as the major virus infecting Brassica crops, often cause severe yield and quality losses in Chinese cabbage production in China. In this study, quantitative trait loci (QTL) analysis for TuMV resistance was conducted with a population of 100 doubled-haploid lines derived from the F 1 between 91-112 (resistant) and T12-19 (susceptible) through microspore culture. A total of 376 molecular markers including 235 amplified fragment length polymorphisms, 129 random amplified polymorphic DNAs, 10 simple sequence repeats, one SCAR and one morphological marker were employed to construct a linkage map with 10 linkage groups covering 809.1 cM with an average distance of 2.2 cM between loci. Resistance was assessed by artificial inoculation at the seedling stage and at the adult stage in field conditions, respectively. Four QTLs controlling TuMV resistance were identified with JoinMap QTL 4.0 and interval mapping method. Two QTLs (Tu1,Tu2) were associated with resistance at the seedling stage, each accounting for 58.2% and 14.7% of the phenotypic variation, respectively. Two QTLs (Tu3,Tu4) were found corresponding to the disease resistance at the adult plant stage, explaining 48.5% and 32.0% of the phenotypic variation, respectively. Marker-assisted selection for these major QTLs involved in TuMV resistance could be useful in Chinese-cabbage breeding programmes.
Four propellers architecture proposed for the Submarine Drone

1Doctoral Student in Electricity Engineering, the National Higher School of Electricity and Mechanics (ENSEM), Morocco 2Dr. Professor, Royal Navy School (ERN) of Morocco, Casablanca, Morocco 3Dr. Professor and Director, the National Higher School of Electricity and Mechanics (ENSEM), Morocco ---------------------------------------------------------------------***---------------------------------------------------------------------Abstract The use of underwater vehicles, which was once an option, has become a necessity in many cases such as research, mapping or monitoring of sea depths. Also, the hostility of the underwater environment made these missions more difficult and more expensive, even impossible in certain cases. The use of these mobile robots offered a very advantageous alternative compared to Human intervention and often more to what the mission requires [1].
Five Years on in SWSE: Experiences from the Semantic Web Search Engine Project

In this paper, we discuss the architecture and implementation of a Semantic Web search engine, appropriately called the Semantic Web Search Engine (SWSE). Following traditional search engine architecture, SWSE consists of crawling, data enhancing, indexing and a user interface for search, browsing and retrieval of information; unlike traditional search engines, SWSE operates over RDF Web data—loosely also known as Linked Data—which implies unique challenges for the system design, architecture, algorithms, implementation and user interface. In particular, many challenges exist in adopting Semantic Web technologies for Web data: the unique challenges of the Web—in terms of scale, unreliability, inconsistency and noise—are largely overlooked by the current Semantic Web standards. In this paper, we detail the current SWSE system, initially detailing the architecture and later elaborating upon the function, design, implementation and performance of each individual component. In so doing, we also give an insight into how current Semantic Web standards can be tailored, in a best-effort manner, for use on Web data. Throughout, we offer evaluation and complementary argumentation to support our design choices, and also offer discussion on future directions and open research questions. Later, we also provide candid discussion relating to the difficulties currently faced in bringing such a search engine into the mainstream, and lessons learnt from roughly five years working on the Semantic Web Search Engine project.
A Novel Spectral Method for Burgers Equation on The Real Line

A spectral method for the Burgers equation on the whole real line based on generalised Hermite functions is proposed. The generalised stability and convergence of the method are proved. Numerical results confirm the theoretical findings and demonstrate the efficiency of the algorithm. AMS subject classifications: 41A30, 76M22, 65M70, 33C45, 65M12
Delft University of Technology Mapping Energy Frames: an Innovative Approach Engaging with New Forms and Types of ‘data’ in Social Media Analysis to Frame Energy Demand

Citation (APA) Mauri, A. , Bozzon, A. , & De Kok, R. (2019). Mapping Energy Frames: an Innovative Approach Engaging with New Forms and Types of ‘data’ in Social Media Analysis to Frame Energy Demand. In F. Savini, B. Pineda Revilla, K. Pfeffer, & L. Bertolini (Eds.), From efficiency to reduction. Tackling energy consumption in a cross disciplinary perspective (pp. 113-147). InPlanning. https://doi.org/10.17418/B.2019. 9789491937439
Isolation and characterization of 10 new compound microsatellite markers for a mangrove tree species, Avicennia marina (Forsk.) Vierh. (Avicenniaceae)

Avicennia marina is an ecologically important mangrove tree species. We isolated 10 polymorphic microsatellite loci from this species using an improved technique. Our isolated loci provided compound microsatellite markers with polymorphism of two to six alleles per locus. The observed and expected heterozygosities ranged from 0.025 to 0.625 and from 0.096 to 0.767, respectively. These markers would be the useful tools for researching on the genetic diversity and population genetic structure of A. marina.
Selectivity, Roach, Rutilus rutilus, SELECT method, Uluabat Lake

The purpose of this study was determine the selectivity properties of gillnet used in Roach (Rutilus rutilus) fishery in the Lake Uluabat. In the fishery works, 7 different gill net which were 100 meters in length and 32, 40,50, 60,70,80 and 90 mmmesh size with a same thickness and depth of 50 mesh as vertically have been used. Field work was carried out monthly in a period of January 2011 and December 2011. Length and weights of 434 samples were determined with 1mm and 1g precision measurement board and scale. SELECT method was used in the determination of the selectivity parameters. Obtained data were analyzed by PASGEAR II v 2.5 computer software. The program calculates parameters of 5 different model (Normal location, normal scale, log-normal, gamma and bi-modal) based on SELECT method. Normal scale yielded the best as a result of analysis. According to the this model optimum catch lengths and spread value of nets which have 32, 40, 50, 60, 70 and80 mm mesh size determined as 12.16-1.24; 15.20-1.56; 19.00-1.95; 22.80-2.34; 26.60-2.73; 30.403. 12 respectively. According to the Kolmogorov-Smirnov test results differences were determined between size frequency distribution of fish caught by all nets.
Transcriptionai induction of Streptomyces cacaoiβ‐lactamase by a β‐lactam compound

The soil bacterium Streptomyces cacaoi produces an extracellular β‐lactamase. The β‐lactamase expression could be induced by the β‐lactam compound 6‐amino penicillinoic acid (6‐APA). In liquid cultures, a 50‐fold increase in β‐lactamase expression was observed within the first three hours after addition of 6‐APA. Using the cloned β‐lactamase gene as a probe, it was shown that this increase was mediated at the level of transcriptional initiation. The start point of the induced β‐lactamase transcript was determined, and the nucleotide sequence of the promoter region was analysed. No noticeable homology was found to control regions of inducible β‐lactamase genes of other bacteria. A striking feature was the presence of six direct repeats (ten base pairs each) upstream of the promoter region. Thus, an example of an inducible regulatory gene system in this Gram‐positive microorganism is presented. Also, the primary structure of the β‐lactamase was deduced, showing a high degree of homology with class A β‐lactamases.
Food stress causes differential survival of socially parasitic caterpillars of Maculinea rebeli integrated in colonies of host and non‐host Myrmica ant species

Final instar larvae of Maculinea rebeli Hirschke (Lepidoptera, Lycaenidae) are social parasites of Myrmica Latreille (Hymenoptera, Formicidae) nests. In the populations of the southern French Alps and Spanish Pyrenees, >95% adult M. rebeli emerge from colonies of Myrmica schencki Emery, despite >60% caterpillars being adopted by other Myrmica species (non‐hosts). However, in laboratory culture caterpillars can be reared successfully by many of the non‐host species. This contradiction, which has led some to question the existence of host specificity, has been explained by the lack of stress, particularly food stress, in laboratory cultures compared to wild conditions. Here, we report the results of an experiment that tested the survival of M. rebeli caterpillars that had been growing well, after being socially integrated into a series of host and non‐host cultures, and were then subjected to a 4‐week period of stress induced by a ‘starvation diet’ estimated to be less than the minimum for ant survival. Significantly more M. rebeli survived in M. schencki cultures than with any of the other Myrmica species (all died in most non‐host cultures). Under a starvation diet, caterpillars are killed and eaten along with dead workers – this never happens under an ample diet – rather than simply starving to death. It was noted that the proportion of young M. rebeli caterpillars that survived initial integration into an ant colony (including some M. schencki colonies) was a good predictor of subsequent survival under starvation conditions. We concluded that two key phases of host specificity exist in the life of this social parasite: initial integration, in which the caterpillar simply has to be accepted into a host society, followed by full integration, when a relatively high hierarchical status within the host society becomes essential for a caterpillar's survival during periods when the host colony is stressed, e.g., by food shortage. This experimental regime provides a useful bioassay for testing host specificity in other populations of Maculinea.
AN OLD WORKFRAME FOR A NEW PROBLEM: A CLASSICAL MODEL FOR UNSUPERVISED ADAPTIVE FILTERING

A classical adaptive filtering model of the problem of instantaneous blind signal separation, or what is formally known as unsupervised adaptive filtering is presented. This classical form helps understanding the well-known superior behaviour of the natural gradient solution to the blind separation problem. A new RLS-based algorithm is developed using this classical model. The algorithm provides improved on-line separation speed under the same steady state error compared to the natural gradient algorithm without requiring pre-whitening.
Disassortative mating for boldness decreases reproductive success in the guppy

Consistent individual differences in personality or temperament have been observed in many animal taxa, and one particular trait, boldness, has been studied extensively. Most studies on mate choice and personality have focused on female preference and have showed that females prefer to mate with bolder males. However, the influence of the female’s personality on this mate preference and on her compatibility with a mate with particular traits has been largely neglected. Here, using the guppy Poecilia reticulata, we investigated the effect of female boldness on mate choice and of the combinations of this trait in the male and female of a mating pair on parturition and brood size. Our results showed that female boldness did not affect mate choice, and brood size was independent of the boldness of the male and female in a pair. However, overall, females who mated with males with a dissimilar degree of boldness to themselves had a lower parturition success than females who mated with males with a similar degree of boldness. This work suggests that the combination of boldness characteristics within a pair influences reproductive success and that individuals of similar personality are more compatible in reproduction. The lower success of disassortative matings is consistent with the hypothesis that variation in personalities is maintained by disruptive or frequency-dependent selection, driven by contrasting physical or social environments that favor alternative rather than intermediate behavioral phenotypes.
Decolourisation of direct dyes with manganese peroxidase from white rot basidiomycete Ganoderma lucidum‐IBL‐5

In the current project the ability of white-rot fungus Ganoderma lucidum IBL-05 was investigated for the decolourisation of some direct textile dyes. In the initial time course study maximum decolourisation (83.78 ±5%) was observed for Solar golden yellow R at pH 4 and temperature 30°C after 6th day. Various process parameters like optimum pH, incubation time, temperature and additional carbon and nitrogen additives were optimized to achieve maximum decolourisation of the dye by G. lucidum IBL-05. Addition of starch (1 %) accelerated the dye decolourisation (96 ± 3%). All the nitrogen sources showed an inhibitory effect on dye decolourisation and enzyme induction. Manganese peroxidase (MnP) was found to be the only enzyme (256 ± 5 U/mL) secreted by G. lucidum IBL-05.
REPRODUCTIVE PERFORMANCE IN EWES ON A LOW-SELENIUM DIET

Sixty-four Selenium (Se)-deficient ewes (group 1) and 65 Se-adequate ewes (group 2) were exposed to fertile, harnessed rams for a 42-day period (1 October–12 November 1973). Between 26 October and 7 December, approximately half of the animals in each group were slaughtered 25–30 days after their last marked mating to determine numbers of embryos and corpora lutea (CL). The percentages of ewes pregnant and CL not represented by normal embryos were 80.6 and 12.8 in group 1 and 78.8 and 34.1 in group 2 and the average number of CL/ewe was 1.57 and 1.63, respectively. The remaining ewes were kept until anticipated time of lambing. The percentage of ewes which lambed and the average number of lambs/ewe were 67.9 and 1.47 (group 1) and 71.9 and 1.44 (group 2). No group differences in sex ratio of lambs was observed. Composite conception rates for the slaughtered and lambed ewes were 76.2% for the Se-deficient and 76.6% for the Se-adequate animals. It was concluded that under the conditions of this experiment Se.
Study of the Size Effect in the Stock Market of Colombia

In this paper we analyze the historical returns of the companies listed on the Colombian stock exchange, from January 2004 until June 2012, in order to determine the possible presence of a risk premium in smaller companies relative to larger ones. This premium is known as Size Effect and it has been found that it is significant after adjusting of risk by Capital Asset Pricing Model. The results obtained in this work for the stock market in Colombia do not show the presence of an additional risk premium due to the size of enterprises, rejecting both size effect and reverse size effect.
Action of Testosterone, Dihydrotestosterone and 5α Androstane 3α, 17β Diol on the Spermatogenesis of Immature Rats

The action of testosterone (T), dihydrotestosterone (DHT) and 5cs-androstane 3a, 1 7� diol (Diol) on the seminiferous epithelium of immature estrogen-treated rats was studied. Quantitative histological evaluation was performed and testicular proteins and androgens contents were determined. Estradiol benzoate (E2B) treatment resulted in a severe testicular atrophy with a reduction of 50 percent in the testicular weight and protein content. In the estrogen treated rats the levels of androgens in the testicular tissue were undetectable. Histologically, the germinal epithelium showed a marked decrease of cell types, particularly the meiotic spermatocytes and young spermatids. In E2Bi-T, E2B+DHT and E2B+Diol-treated animals testicular weight decreased only 10 to 20 percent, with a normal concentration of testicular proteins. In E2B+T-treated animals the three androgens were found in the testis, and in both E2B+DHT and E2B+Diol-treated rats, high levels of these androgens were found, but testosterone was not detectable. In every case the levels of Diol were higher than those of DHT. A diminution of spermatogonia, similar to that found in E2 B-treated animals was noted in all androgen-treated rats. Meiotic spermatocytes showed, however, a significant increase in relation to E2B-treated rats, even though a decrease was observed when compared with the normal controls. Spermatid diminution was only partially prevented in E2B#{247}DHTand E2B+Diol-treated groups, but not in E2B+T treated rats. It is concluded that T, DHT and Diol, exert a meiotic stimulatory effect. In the case of DHT and Diol-treated animals, absence of testicular testosterone indicates that both Sn reduced metabolites are biologically active in the prevention of testicular atrophy induced by E2B at the time of initiation of spermatogenesis.
On Four Endemic Genera of Tasmanian Acrididae (Orthoptera)

Two endemic Tasmanian genera of catantopine Acrididae, Truganinia and Tasmanalpina, are newly described, and two others, Russalpia Sjostedt and Tasmaniacris Sjostedt, redescribed. All but Russalpia (two species) are monotypic, and all are micropterous except Truganinia (mesopterous). The genera are closely related to some of the endemic New Zealand Acrididae, especially in the male genitalia, which must have suffered little modification over the past 50 million years. The species are described and figured, and their distributions plotted and interpreted in relation to historical geographic and climatic changes.
Strudel: Learning Structured-Decomposable Probabilistic Circuits

Probabilistic circuits (PCs) represent a probability distribution as a computational graph. Enforcing structural properties on these graphs guarantees that several inference scenarios become tractable. Among these properties, structured decomposability is a particularly appealing one: it enables the efficient and exact computations of the probability of complex logical formulas, and can be used to reason about the expected output of certain predictive models under missing data. This paper proposes Strudel, a simple, fast and accurate learning algorithm for structured-decomposable PCs. Compared to prior work for learning structured-decomposable PCs, Strudel delivers more accurate single PC models in fewer iterations, and dramatically scales learning when building ensembles of PCs. It achieves this scalability by exploiting another structural property of PCs, called determinism, and by sharing the same computational graph across mixture components. We show these advantages on standard density estimation benchmarks and challenging inference scenarios.
Clustering of Multidimensional Objects in the Formation of Personalized Diets

When developing personalized diets (personalized nutrition) it is necessary to take into account individual physiological nutritional needs of the body associated with the presence of gene polymorphism among consumers. This greatly complicates the development of rations and increases their cost. A methodology for the formation of target diets based on the multidimensional objects clustering method has been proposed. Clustering in the experimental group was carried out on the basis of a calculation of the integral assessment of reliable risks of developing decease conditions according to selected metabolic processes. And genetic data of participants was taken into account. The use of the proposed method allowed reducing the needed number of typical solutions of individual diets for the experimental group from 10 to 3.
A new nature inspired modularity function adapted for unsupervised learning involving spatially embedded networks: A comparative analysis

Unsupervised machine learning methods can be of great help in many traditional engineering disciplines, where huge amount of labeled data is not readily available or is extremely difficult or costly to generate. Two specific examples include the structure of granular materials and atomic structure of metallic glasses. While the former is critically important for several hundreds of billion dollars global industries, the latter is still a big puzzle in fundamental science. One thing is common in both the examples is that the particles are the elements of the ensembles that are embedded in Euclidean space and one can create a spatially embedded network to represent their key features. Some recent studies show that clustering, which generically refers to unsupervised learning, holds great promise in partitioning these networks. In many complex networks, the spatial information of nodes play very important role in determining the network properties. So understanding the structure of such networks is very crucial. We have compared the performance of our newly developed modularity function with some of the well-known modularity functions. We performed this comparison by finding the best partition in 2D and 3D granular assemblies. We show that for the class of networks considered in this article, our method produce much better results than the competing methods.
Increasing HMOX-1 Expression with SB cells® Treatment Ameliorates Chronic Hypertension

Hypertension has been known to be one of the most common chronic inflammations of the body affecting lives of many worldwide. Constant elevation of blood pressure limits the organ from performing at its optimal rate, and its effects are more widespread throughout the body. Many prescription drugs have been created and used by affected individuals at a cost-severe financial loss and/or inescapable side effects. The race to discover other alternative treatment leads to newfound attention to stem cell therapy where patients are treated with their own stem cells in the hope of minimizing cost and side effects. The intention of our study is to use SB cells® cells to approach hypertension syndromes by testing the gene Heme oxygenase decycling-1 (HMOX-1). HMOX-1 is a critical enzyme that breaks down heme to produce carbon monoxide (CO). CO is an important metabolite that participates in vasodilation and many other anti-inflammation processes. We observed a significant increase in HMOX-1 expression in the blood 24-hours and/or 1-week post-SB cells® treatment. Additionally, blood pressure level of tested patients significantly decreased post treatment. We envision that this application could one day be used to treat other HMOX1 related diseases such as inflammation as well as cancer.