Miracle-dz/UniFAR-FTUs huggingface dataset:
Design and Simple Assembly of Gold Nanostar Bioconjugates for Surface-Enhanced Raman Spectroscopy Immunoassays

Immunoassays using Surface-Enhanced Raman Spectroscopy are especially interesting on account not only of their increased sensitivity, but also due to its easy translation to point-of-care formats. The bases for these assays are bioconjugates of polyclonal antibodies and anisotropic gold nanoparticles functionalized with a Raman reporter. These bioconjugates, once loaded with the antigen analyte, can react on a sandwich format with the same antibodies immobilized on a surface. This surface can then be used for detection, on a microfluidics or immunochromatographic platform. Here, we have assembled bioconjugates of gold nanostars functionalized with 4-mercaptobenzoic acid, and anti-horseradish peroxidase antibodies. The assembly was by simple incubation, and agarose gel electrophoresis determined a high gold nanostar to antibody binding constant. The functionality of the bioconjugates is easy to determine since the respective antigen presents peroxidase enzymatic activity. Furthermore, the chosen antibody is a generic immunoglobulin G (IgG) antibody, opening the application of these principles to other antibody-antigen systems. Surface-Enhanced Raman Spectroscopy analysis of these bioconjugates indicated antigen detection down to 50 µU of peroxidase activity. All steps of conjugation were fully characterized by ultraviolet-visible spectroscopy, dynamic light scattering, ζ-Potential, scanning electron microscopy, and agarose gel electrophoresis. Based on the latter technique, a proof-of-concept was established for the proposed immunoassay.
Change, Coordination, and Capabilities

Empirical studies on coordination of economic activities focused on the two polar cases of governance mode, namely vertical integration and market exchanges. Whether firms should be vertically integrated or lever market exchanges in the face of change is, however, debated. Two positions have emerged. Some scholars argue that the vertically integrated firm is the appropriate mode of coordination when change occurs, while market exchanges are more appropriate for dealing with stable contexts (Teece, 1996). On the other hand, Harrigan (1984, 1985) contends that firms should rely on market exchanges when technological change renders upstream capabilities obsolete. Based on two case studies of the aircraft engine industry, this paper introduces the concept of systems integration as the primary coordination mechanism in-between markets and hierarchies that firms employ to cope with change. The focus is on multitechnology settings. Multitechnology, multicomponent products intensify the coordination efforts for firms developing them and therefore provide a vantage point to study coordination modes in the face of technological change. The paper argues that systems integration, as a coordination mechanism, comprises a set of different technological and organizational skills, ranging from component assembly through the understanding and integration of the technological disciplines underlying a product, to project management. It shows that from a competitive point of view, systems integration is most appropriately understood as knowledge integration. Systems integrating firms are understood as those organizations that set up the network of actors involved in the industry and lead it from an organizational and technological viewpoint.
Specifications as a Barrier to the Transfer and Utilization of New Technology into Navy Construction: A Critical Review.

Abstract : The possibility that the system of specifications presently followed by Naval Facilities Engineering Command (NAVFAC) acts as a significant barrier to the transfer and utilization of new technology into Navy construction is analyzed. Specific areas are investigated using personal interviews and emphasis is placed on identifying and understanding the barriers and to improve the efficiency of technology transfer within the Navy.
Reliable Estimation of Density Distribution in Potential Wind Power Sites of Bangladesh

This paper proposes techniques to reliably estimate density distribution (DD) of wind power in six test sites of Bangladesh selected by the UN showing potential for harnessing wind energy. Wind power profile rule (with constant and variable power coefficients) and logarithmic variation of wind shear are applied to seven unique methods used in forecasting airstream energy density achievable in the country at commercial turbine axleheights (30-120m). The problems related to fixating the power law coefficient in a site against environmental and geographic factors are identified while predicting wind profiles from data measured at low-altitude (~10m). Relative deviation in density distribution forecasted by the techniques is calculated to identify that among the proposed methods, wind power rule with profile factors varying against altitude produces rates of inconsistency lower than 3% over a turbine range of 40-90m and results obtained by a logarithmic method stays below 12% in the same range. Projected wind profiles in Bangladesh achieve a maximum power class level of four and a power density coverage of 30-400 Watt/m2, sufficient for medium scale (above 20kW) grid-supported wind projects. This study intends to evaluate the potential of the concerned country for integrating stand-alone wind-farms which could boost its struggling rural energy sector.
Inorganic Semiconductors for Light‐emitting Diodes

During the past 40 years, light-emitting diodes (LEDs) have undergone a significant development. The first LEDs emitting in the visible wavelength region were based on GaAsP compound semiconductors with external efficiencies of only 0.2%. Today, the external efficiencies of red LEDs based on AlGaInP exceed 50%. AlGaInP semiconductors are also capable of emitting at orange, amber, and yellow wavelengths, albeit with lower efficiency. Semiconductors based on AlGaInN compounds can emit efficiently in the UV, violet, blue, cyan, and green wavelength range. Thus, all colors of the visible spectrum are now covered by materials with reasonably high efficiencies. This opens the possibility to use LEDs in areas beyond conventional signage and indicator applications. In particular, LEDs can now be used in high-power applications thereby enabling the replacement of incandescent and fluorescent sources. LED lifetimes exceeding i 10 h compare favorably with incandescent sources (Z 500 h) and fluorescent sources (Z 5000 h), thereby contributing to the attractiveness of LEDs. Inorganic LEDs are generally based on p-n junctions. However, in order to achieve high internal quantum efficiencies, free carriers need to be spatially confined. This requirement has led to the development of heterojunction LEDs consisting of different semiconductor alloys and multiple quantum wells embedded in the light-emitting active region. The light-extraction efficiency, which measures the fraction of photons leaving the semiconductor chip, is strongly affected by the device shape and surface structure. For high internal-efficiency active regions, the maximization of the light-extraction efficiency has proven to be the key to highpower LEDs. This chapter reviews important aspects of inorganic LED structures. Section 1.2 introduces the basic concepts of optical emission. Band diagrams of direct and indirect semiconductors and the spectral shape of spontaneous emission will be discussed along with radiative and nonradiative recombination processes. Spontaneous emission can be controlled by placing the active region in an optical 1
Managing Staff Development

Staff development: what is it and how has it evolved? The manager's role in and responsibilities for staff development The organization of staff development in departments Managing staff development for learning and teaching Managing staff development for research and scholarship Managing staff development for leadership, management and administration Managing staff development for the use of new technology Achieving a holistic and integrated approach to staff development Evaluating staff development and harnessing its potential References Index.
Analysis and Prediction of Differential Parking Behaviors

Nowadays, most modern parking lots integrate IoT technologies such as license plate recognition, mobile payment and automatic entrance/exit control to improve the convenience of the drivers and parking lot managers. However when searching for a vacant parking space becomes normally difficult in a metropolitan area, a more advanced "Smart Parking System" that can predict vacancy, enable reservation and differentiate pricing is even more needed. In this sense, understanding the parking behavior of customers has great significance to the manager of the parking estate, the drivers and the urban administrations. This paper explores the patterns and predictability of differential parking flows. With the license plate recognition and mobile payment system deployed in the parking lots, we collect about 14 million parking records of more than 300 parking lots in a big metropolitan in China. The time of every vehicle entering and exiting the parking lot is recorded along with its plate number. We first extract some features from these records and use K-means cluster algorithm to categorize the vehicle-parking lot pairs into three clusters empirically. The three clusters of parking behaviors are interpreted as regular parking users, long time visiting users and short time visiting users. Secondly, based on the parking lot's historical occupancy patterns, we designed several methods to predict the occupancy of the parking lot for different types of parking. The occupancies from the three types of vehicles can be used for making differential pricing policies or reservation policies.
Traffic signal timing manual.

This report serves as a comprehensive guide to traffic signal timing and documents the tasks completed in association with its development. The focus of this document is on traffic signal control principles, practices, and procedures. It describes the relationship between traffic signal timing and transportation policy and addresses maintenance and operations of traffic signals. It represents a synthesis of traffic signal timing concepts and their application and focuses on the use of detection, related timing parameters, and resulting effects to users at the intersection. It discusses advanced topics briefly to raise awareness related to their use and application.
Instructional Techniques and Practice

It is written to suit the needs of a variety of groups: industrial training staff, supervisors, teachers and others concerned with training and education.It also enables company staff to acquire a sufficient working knowledge of instructional techniques to prepare them for taking up appointments as leading hands, section leaders and supervisors, both in industry and commerce.
Geotechnical Characterization of the C.A.S.E. project sites

The paper summarises the experimental data collected by in-situ and laboratory tests on the soils in the Aterno valley, with the aim to characterize the seismic response of the sites selected for the emergency houses (C.A.S.E.), built few months after the April 6, 2009 L’Aquila earthquake. The results reported in the paper are relevant to the experimental activity coordinated by the Italian Geotechnical Society (AGI), and carried out by a network of advanced soil dynamics laboratories. The in situ tests consisted of MASW tests; the shear wave velocity profiles in some sites were successfully compared against DH and SDMT tests and instrumentally recorded HVSR amplification. The laboratory investigation included cyclic/dynamic simple/torsional shear tests on undisturbed samples. The results, summarised in terms of variation of stiffness and damping with shear strain, highlighted some peculiar properties of the soils in the Aterno valley, which can significantly affect the non-linear seismic response of the sites.
The impact of entrepreneurship on economic development through government policies and citizens’ attitudes

Abstract This research aims to investigate the field of entrepreneurship in the context of public sector governance in eight of the largest economies in the world (the G7 countries and Russia). To analyse the composition and evolution of entrepreneurship, data from the Global Entrepreneurship Monitor was collected, while the economic stability was based on GDP data from the World Bank. To understand the relationships between the public sector governance policies and attitudes towards entrepreneurship in terms of economic development, the 2001-2018 period was considered. The relationships studied were observed through correlation and regression analyses, based on indexes obtained through principal component analysis. Results indicate that there are strong positive correlations between GDP and cultural and social norms promoted in society, total early-stage entrepreneurial activity, physical and services infrastructure, and tax and bureaucracy, while the fear of failure affects the GDP. Besides, this research emphasises the fact that individuals’ entrepreneurial attitudes and behaviour may reduce the level of GDP, while the entrepreneurial framework developed by the public sector would have an important role in increasing economic stability.
A Multi Fusion Data Mining Algorithm for Solar Energy Efficiency

The output power of renewable energy has thecharacteristics of random fluctuation, which have the harmfuleffect on stability of renewable power grid and causes the problemof low utilization ratio on renewable energy output power. Thus,this paper proposed a method to predict the output power ofrenewable energy based on data mining technology. Data miningis performed using linear regression algorithm, decision tree, andrandom forest. The simulation experiment results show thevariation of solar radiation size and inclination angle, whichimproves solar panel position control accuracy and solar energyutilization in solar photovoltaic power generation systems. Andthis provides the scientific basis for theory and application of theefficiency of utilizing solar energy.
Reliability considerations in the design of cellular manufacturing systems: A simulated annealing‐based approach

Purpose – The purpose of this paper is to: develop an effective cellular manufacturing system (CMS) design methodology by simultaneously considering system costs and individual machine reliabilities; and propose a combinatorial search‐based solution procedure to solve large‐sized problems.Design/methodology/approach – This paper presents a multi‐objective mixed integer‐programming model for the design of CMS with the objective of minimizing costs and maximizing system reliability. The approach optimizes inter‐cell material handling costs, the variable cost of machining operations, and the machine under‐utilization costs. It also maximizes the system reliability by selecting process routes for the part types with the highest system reliability for the machines along the routes. To solve the multi‐objective, multiple process plan model, a simulated annealing (SA)‐based algorithm is developed. The algorithm follows the basic steps of SA, but also incorporates the genetic algorithm (GA) operations of crossove.
Impact of modulation of telomerase and cancer stem‐cell marker OCT4 axis in cervical cancer pathogenesis with underlying HPV16 infection

Lacunae exist in the molecular event(s) specificity associated with cervical cancer (CaCx) pathogenesis. The present study aimed to evaluate the significance of telomerase‐cervical cancer stem cells (CSCs) modulation in CaCx pathogenesis with underlying HPV16 infection. The study included HPV16 positive cases only (N = 65) of the total enrolled cases from Northeast India. The analysis of viral load and the differential messenger RNA expression of E6, E7, hTERT, hTR, and cancer stem‐cell markers was studied by real‐time polymerase chain reaction. Further the protein and colocalization study for E6, hTERT, and oct4 was performed by immunofluorescence. The real‐time polymerase chain reaction based analysis showed an upregulation of HPV16 viral oncoprotein E6 and E7, and telomerase component hTERT and hTR expression and their correlation in CaCx susceptibility and severity. The hTERT expression correlated with viral load; while the E6 and telomerase protein expression colocalized in the nucleus. The CSCs marker octamer‐binding transcription factor 4 (OCT4) was significantly upregulated in CaCx cases, was associated with CaCx susceptibility and severity, and colocalized with E6 expression in the nucleus as revealed from the immunofluorescence studies. To conclude, the telomerase‐OCT4 axis modulation holds key in HPV16 CaCx pathogenesis mediated by HPV16 E6 viral oncoprotein expression, and underlines its potential for therapeutic targeting.
Function of the MYND Domain and C-Terminal Region in Regulating the Subcellular Localization and Catalytic Activity of the SMYD Family Lysine Methyltransferase Set5

SMYD lysine methyltransferases target histones and nonhistone proteins for methylation and are critical regulators of muscle development and implicated in neoplastic transformation. They are characterized by a split catalytic SET domain and an intervening MYND zinc finger domain, as well as an extended C-terminal domain. Saccharomyces cerevisiae contains two SMYD proteins, Set5 and Set6, which share structural elements with the mammalian SMYD enzymes. ABSTRACT SMYD lysine methyltransferases target histones and nonhistone proteins for methylation and are critical regulators of muscle development and implicated in neoplastic transformation. They are characterized by a split catalytic SET domain and an intervening MYND zinc finger domain, as well as an extended C-terminal domain. Saccharomyces cerevisiae contains two SMYD proteins, Set5 and Set6, which share structural elements with the mammalian SMYD enzymes. Set5 is a histone H4 lysine 5, 8, and 12 methyltransferase, implicated in the regulation of stress responses and genome stability. While the SMYD proteins have diverse roles in cells, there are many gaps in our understanding of how these enzymes are regulated. Here, we performed mutational analysis of Set5, combined with phosphoproteomics, to identify regulatory mechanisms for its enzymatic activity and subcellular localization. Our results indicate that the MYND domain promotes Set5 chromatin association in cells and is required for its role in repressing subtelomeric genes. Phosphoproteomics revealed extensive phosphorylation of Set5, and phosphomimetic mutations enhance Set5 catalytic activity but diminish its ability to interact with chromatin in cells. These studies uncover multiple regions within Set5 that regulate its localization and activity and highlight potential avenues for understanding mechanisms controlling the diverse roles of SMYD enzymes.
Training to Proficiency

People around the world are living longer but are also experiencing conditions that can make personal mobility a challenge. When no longer able to travel independently, this population group must rely on the transit services offered through public transportation and/or specialized transit. The conditions that prohibit independent transport are usually the result of frailty or some form of disability. The public transportation and/or specialized transit service providers need to be trained to be considerate of this vulnerable population. First, all service providers need human relations training as may be necessary to ensure that individuals who are frail and/or have disabilities are treated with dignity and respect. A higher level of care must be exerted with a clear understanding of differences between people based on capabilities as well as needs. This is called sensitivity training. Second, all service providers need technical training in assisting people to get from one place to the next. This technical skills training should cover such areas as safe vehicle operation, use of lift/ramp equipment, procedures for proper securement and restraint, and appropriate methods used in physically assisting individuals who need help in getting on/off vehicles and to/from facilities. This training must be designed so that employees understand their responsibilities for all passengers who are frail and/or with disabilities and not only those passengers who use wheelchairs. This is called passenger assistance training. This paper will stipulate a method with procedures to follow based upon job instruction training. It will elaborate on the following five points as related to sensitivity and passenger assistance training (1) Prepare to train, (2) Select and prepare the drivers, (3) Train, (4) Performance, (5) On the job performance. In order for skills to be transferred during training, the paper will recommend a set of procedures to be followed based on the six steps that follow: (1) Perform a task analysis, (2) Set a conducive learning environment, (3) Tell the attendees how to do the task, (4) Show the attendees how to do the task, (5) Allow the attendees to do the task, (6) Review their behavior in ways that reinforce their achievements and set goals for improvement. Through the utilization of these guidelines, competence and excellence can be achieved. Training with these guidelines can ensure that service personnel are trained to proficiency, as appropriate to their duties, so that they are able to operate equipment safely and properly assist and treat individuals who depend upon the service in a respectable and courteous manner, with appropriate attention to the differences among individuals who are frail and/or with disabilities.
Moving beyond stepped care to staged care using a novel, technology‐enabled care model for youth mental health

A ustralia can rightly claim to lead the world in mental health awareness, especially for the mental health and wellbeing of young people.1 However, despite the development of designated primary carestyle services (eg, headspace),1 we still do not deliver effective care, early in the course of illness, to most young people with anxiety, depression, or alcohol or other substance misuse.2 Even when we do deliver care, the longer term functional outcomes are often disappointing.3 The consequences of this failure remain large — personally, socially and economically.4
ATP4B suppresses cell proliferation and migration via mitochondrial/p53/NF-κB pathway in gastric cancer

Background: Hydrogen/Potassium ATPase β (ATP4B) is a key protein in gastric mucosa barrier acting an essential role in gastric acid secretion. However, the exact role and precise mechanism of ATP4B in gastric cancer (GC) remain obscure. This study aimed to investigate the clinical significance of ATP4B in GC and its biological role in tumor progression.Methods: We evaluated ATP4B expression in GC cell lines and patient specimens via qPCR and Immunofluorescence. The correlations between ATP4B expression level and clinicopathologic parameters, as well as the relevance of ATP4B expression with overall survival were assessed. The functional roles of ATP4B in GC were verified by gain- and loss-of-function cell models and xenograft tumor model. The possible downstream effects of ATP4B were analyzed by iTRAQ‑based quantitative proteomics analysis.Results: A dramatic decrease of ATP4B expression in GC cells and tissues compared with the adjacent normal tissues was observed; Downexpression of ATP4B was associated with the malignant transformation in gastric mucosa lesions and correlated with poor prognosis, high grade of TNM stage, and vessel carcinoma embolus in GC patients. Restoration of ATP4B expression in GC cells significantly inhibited cell proliferation, cell viability, migration, invasion, tumorigenicity and induced apoptosis, whereas ATP4B silencing exerted the opposite effects. Mechanistically, we found a constitutive activation of p53 and inactivation of NF-κB signaling correlated with the mitochondrial pathway in ATP4B-overexpressing GC cells.Conclusion: Our data suggest that ATP4B perform the promising tumor suppressor gene by regulating p53/NF-κB/mitochondrial pathway in GC.