Miracle-dz/UniFAR-FTUs huggingface dataset:
Direct Detection and phylogenetic analysis of Streptococcus agalactiae from raw milk damples in Mashhad-Iran

Introduction Streptococcus agalactiae (S. agalactiae), also known as Lance field Group B. Streptococcus (GBS), is an infectious pathogen that can introduce significant economic damage to the dairy industry on account of its detrimental effects on bovine udder health that is noticeable by serious problems in productivity and milk quality (Pinar, 2004). This bacterium is also known to cause disease in children, women, the immune -compromised adult, and the elderly (Amaya et al., 2004). It has rarely been isolated from dogs, horses, guinea pigs and fish (Elliott et al., 1990). S. agalactiae falls into the pyogenic group and they are recognized on blood agar plates by the classical zone of β hemolysis surrounding colonies. Five serotypes (Ia, Ib, II, III and V) are responsible for 85% of neonatal GBS disease throughout the world and the distribution of these serotypes is the same across all WHO regions (Edmond et al., 2012). Colonization is of crucial importance sinceit appears to precede invasive disease. GBS has been isolated from the rectum, perianal area, vagina, cervix and urethra (Melin, 2011). It is known that the vaginal flora can be a key factor in pregnancy outcome and GBS colonisation is connected with previous spontaneous abortion (Rocchetti et al., 2010). A surveillance and control program for S. agalactiae was established in 1966 with the ultimate goal of eradicating infections with the pathogen from Abstract Advances in the science of molecular biology have led the way into a new area by allowing direct detection and identification of pathogenic microorganisms. This study attempted to develop a simple and specific procedure for direct identification of Streptococcus agalactiae from cow's raw milk samples in Mashhad-Iran. The method was used based on polymerase chain reaction (PCR) using species-specific primers derived from the 16S rRNA gene. Ten samples were sequenced by forward and reverse primers and phylogenetic analysis for genetics similarly of GBS was done by bioinformatics tools. The amplification product was verified by sequencing. Specific identification proved on a collection of 30 suspicious samples of bovine milk. Results showed that 44% of the samples were contaminated by S. agalactiae. Comparison of the sequences obtained from the mentioned tool revealed that only in position 226, there was a replacement of a nucleotide T by a nucleotide A. The developed PCR test, which is highly specific and sensitive, can be performed within hour’s and provides an advanced diagnostic tool intended for direct detection of S. agalactiae in milk.
Combined Security and Service Overloading Fusion Model for Cloud Environment

: Cloud computing is a field which has been rapidly developing over the past few years. The fact that cloud can offer both storage and computation at low amounts makes it popular among corporations and IT industries. This also makes it a very attractive proposition for the future. But in spite of its promise and potential, security in the cloud proves to be a cause for concern to the business sector. This is due to the outsourcing of data onto third party managed cloud platform. These security concerns also make the use of cloud services less flexible. In this paper, we propose a secure service overloading framework that allows data to be stored securely in the cloud while at the same time allowing operations to be performed on it without any compromise of the sensitive parts of the data.
Effects of Protein Supply from Soyhulls and Wheat Bran on Ruminal Metabolism, Nutrient Digestion and Ruminal and Omasal Concentrations of Soluble Non-ammonia Nitrogen of Steers

Three beef steers fitted with permanent cannulae in the rumen and duodenum were used to determine the effects of protein supply from soyhulls (SH) and wheat bran (WB) on ruminal metabolism, blood metabolites, nitrogen metabolism, nutrient digestion and concentrations of soluble non-ammonia nitrogen (SNAN) in ruminal (RD) and omasal digesta (OD). In a 3×3 Latin square design, steers were offered rice straw and concentrates formulated either without (control) or with two brans to increase crude protein (CP) level (9 vs. 11% dietary DM for control and bran-based diets, respectively). The brans used were SH and WB that had similar CP contents but different ruminal CP degradability (52 vs. 80% CP for SH and WB, respectively) for evaluating the effects of protein degradability. Ruminal ammonia concentrations were higher for bran diets (p 20%). (Key Words : Soyhulls, Wheat Bran, Soluble non- ammonia Nitrogen, Omasal Digesta, Nutrient Digestion)
he effect of probe inaccuracies on the quantitative model-based nalysis of high angle annular dark field scanning transmission lectron microscopy images

Quantitative structural and chemical information can be obtained from high angle annular dark field scanning transmission electron microscopy (HAADF STEM) images when using statistical parameter estimation theory. In this approach, we assume an empirical parameterized imaging model for which the total scattered intensities of the atomic columns are estimated. These intensities can be related to the material structure or composition. Since the experimental probe profile is assumed to be known in the vailable online 27 December 2013
Mobile learning through indigenous languages : learning through a constructivist approach

An increase in accessible electronic pedagogy available in African languages is vital in extending learning resources to resource constrained schools and multilingual communities. The lack of appropriate mobile and electronic resources in these contexts often limits learners from participating in the digital age and discovering knowledge through technology. South Africa, used as the context for this study, has eleven official languages and promotes their use for education in schools. There are however limited bilingual resources that support bilingual education. The different challenges, such as the limited resources that enable an effective learning environment, often make it difficult for teachers to create new learning environments that embrace technology in classrooms. This paper presents a case study that evaluates the potential use of mobile learning to support the current informal learning process for resource constrained multilingual high school learners. In the study learners created their own audio based bilingual learning material on mobile phones using a constructivist learning approach, in the subject area of physical science. A total of 32 high school learners and their teacher from a South African school participated in the study, and though semi–structured interviews and questionnaires learners reported on their experiences. Infrastructure challenges, including limited access to free electronic resources, and slow and expensive Internet access, became the main hurdles in supporting a constructivist learning environment. The mobile learning process however gave learners an opportunity to create their learning content in their own languages at any location at any time, and use the content later for revision. Read More: http://www.qscience. com/doi/abs/10.5339/qproc.2013. mlearn.14
A high repetition deterministic single ion source

We report on a deterministic single ion source with high repetition rate and high fidelity. The source employs a magneto-optical trap, where ultracold rubidium atoms are photoionized. The electrons herald the creation of a corresponding ion, whose timing information is used to manipulate its trajectory in flight. We demonstrate an ion rate of up to 4 × 10 4 s − 1 and achieve a fidelity for single ion operation of 98%. The technique can be used for all atomic species, which can be laser-cooled, and opens up new applications in ion microscopy, ion implantation and surface spectroscopy.
Rinjani and Propok Volcanics as a Heat Sources of Geothermal Prospects from Eastern Lombok , Indonesia

The eruptive history of Sembalun–Propok-Rinjani volcanic complexes during the young Quaternary age had been studied. Those young volcanic complexes are as a good geothermal heat source of the Eastern Lombok prospects. The ages of Sembalun volcanic rock complex were formed during the last of 0.45 Ma. The pyroclastics of youngest Rinjani volcanic products was formed during a stratocone building stage between 0.012-0.006 M.a. The volcanic activity migrated more 5 km eastward for a low activity stage, erupting Propok pumice and Lembar dacitic lava flows. The Propok, Lembar and Rinjani volcanics seems to be as an up flow zone and good heat sources for a geothermal prospect of eastern Lombok Island. These areas are recognized by 10 Ohm-m resistivity zones of low frequency MT data and CSMT profiles. Out flow of neutral warm springs (25-38C) discharge at the eastward and northeast, approximately 2 km of the Propok craters. Therefore, these information may give an early assessment of Lombok geothermal prospects.
Sliding mode control strategy for variable speed wind turbines

The efficiency of the wind power conversions systems can be greatly improved using an appropriate control algorithm. In this work, a robust control for variable speed wind power generation that incorporates a doubly feed induction generator is described. The electrical systems incorporates a wound rotor induction machine with backto-back three phase power converter bridges between its rotor and the grid. In the presented design it is applied the so called vector control theory. The proposed control scheme uses stator flux-oriented control for the rotor side converter bridge control and grid voltage vector control for the grid side converter bridge control. The proposed robust control law is based on a sliding mode control theory, that, as it is well known, presents a good performance under system uncertainties. The stability analysis of the proposed controller under disturbances and parameter uncertainties is provided using the Lyapunov stability theory. Finally simulated results show, on the one hand that the proposed controller provides high-performance dynamic characteristics, and on the other hand that this scheme is robust with respect to the uncertainties that usually appear in the real systems.
Relationship between personality type and fruit and vegetable preference in third and fourth grade students

Objective: The purpose of this study was to investigate the relationship between personality type and fruit and vegetable preferences of third and fourth grade children. Methods: A cross-sectional, quantitative survey study was used to gather information from parents or caregivers (N=345). Survey packets were distributed and included a demographic questionnaire, the Inventory of Children's Individual Differences (short version), the Fruit and Vegetable Preference Questionnaire, and the Healthy Eating Index (HEI). Demographic variables were controlled and multiple regression analyses were conducted for fruit preference, vegetable preference, fruit and vegetable preference, and the HEI score to determine relationships to openness to experience, agreeableness, and neuroticism. Results: The combined effect of openness to experience, agreeableness and neuroticism was significant for the fruit preference score (p<0.05), vegetable preference score (p<0.001), combined fruit/vegetable preference score (p<0.01), and the HEI score (p<0.01). Conclusion: These results indicate that children who are more agreeable and open in nature may have stronger preferences for fruits, vegetables, and fruits and vegetables in general, and they may score higher on the HEI, and children who are more neurotic may have lower preferences for fruits and vegetables, and they may score lower on the HEI. These results support the consideration of personality as a factor that influences food preference.
Modelling Energy Spot Prices by Lévy Semistationary Processes

This paper introduces a new modelling framework for energy spot prices based on Levy semistationary processes. Levy semistationary processes are special cases of the general class of ambit processes. We provide a detailed analysis of the probabilistic properties of such models and we show how they are able to capture many of the stylised facts observed in energy markets. Furthermore, we derive forward prices based on our spot price model. As it turns out, many of the classical spot models can be embedded into our novel modelling framework.
Early Cellular Responses of Purine Nucleoside-mediated Protection of Hypoxia-induced Injuries of Neuronal PC12 Cells

Hypoxia in brain may lead to cell death by apoptosis and necrosis. In parallel adenosine, a powerful endogenous neuroprotectant is formed. We wanted to investigate the effect of adenosine and its purine nucleoside relatives, inosine and guanosine on early cellular responses to hypoxia. O2-sensitive neuronal PC12-cells were subjected to chemical hypoxia induced with rotenone, an inhibitor of mitochondrial complex I. Loss of viability after hypoxic insult was impressively rescued by adenosine, guanosine and inosine. PC12-cells mainly express the A2A adenosine receptor. Its inhibition with a specific antagonist (CSC) induced cell death of PC12-cells, which could be salvaged by adenosine but not with guanosine or inosine. We have previously demonstrated the important role of mitogen activated protein kinases 1/2 (p42/44 MAPK) in purine-mediated rescue. In this study we were interested in the involvement of protein kinases whose activities mediate these processes, including protein kinase A (PKA), phosphoinositide 3-kinase (PI3-K) and protein kinase C-related kinases (PRK 1/2). Pharmacological inhibition of PKA and PI3-K increased hypoxia-induced toxicity and likewise also affected the rescue by purine nucleosides. Nerve growth factor (NGF) and purine nucleosides induced an activation of PRK 1/2, which to our knowledge indicates for the first time that these kinases are potentially involved in purine nucleoside-mediated rescue of hypoxic neuronal cells. Results suggest that A2A receptor expressing cells are mainly dependent on the purine nucleoside adenosine for their rescue after hypoxic insult. In addition to PKA, PI3-K is an important effector molecule in A2A-mediated signaling and for the rescue of PC12-cells after hypoxic insult.
Historical reconstruction of monthly oceanic precipitation (1900–2006)

[1] An oceanic precipitation reconstruction is developed and evaluated for periods extending back to as early as 1900. Large-scale monthly oceanic precipitation is reconstructed using the available network of historical gauge data fit to a set of large-scale covariance spatial modes based on modern analysis. The modern analysis is based on satellite microwave estimates merged with atmospheric reanalysis estimates. For 1900–2006, a monthly and 5° spatial product is developed for reconstructing climate-scale (large-scale spatial and inter-seasonal and longer timescales) variation in pre-satellite periods. Advantages of this study include the use of a homogeneous satellite analysis for computing reconstruction statistics and improved tuning of the reconstruction methods. Cross-validation testing is used to show how much historical variance can be reconstructed with the available sampling. In addition, reconstructions are performed using several different gauge data sets to evaluate differences due to the choice of gauge data. Testing shows that that these improved methods produce reconstructions with useful skill in the tropics and Northern Hemisphere midlatitudes. In the Southern Hemisphere midlatitudes and in polar latitudes in both hemispheres, sampling is too sparse to yield much skill. The reconstruction is able to resolve large-scale modes of variation useful for some diagnostic studies of the 20th century. Reconstruction efforts will continue as improved satellite and atmospheric reanalyses become available.
Development of a Capillary Electrophoresis Chip with Integrated Electrochemical Sensors

Capillary electrophoresis (CE) chips with amperometric integration offers improved portability, high detection throughput, small sample and reagent volumes, low driving voltages and sample injection and separation. This article reviews recent CE chip developments and applications in the integration of amperometric sensors. We describe the development of three different configurations (in-channel, off-channel and end-channel). CE chips with integrated EC detectors have great promise in applications including diagnostics, environmental monitoring and biological assays
Inextricable complexity of two centuries of demographic changes: A fascinating modeling challenge

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Inextricable complexity of two centuries of demographic changes: A fascinating modeling challenge Nicole El Karoui, Kaouther Hajji, Sarah Kaakai
In Vitro Propagation of Tylophora indica-Influence of Explanting Season, Growth Regulator Synergy, Culture Passage and Planting Substrate

An efficient protocol for rapid clonal propagation of an endangered medicinal plant, Tylophora indica (Burm. f.) Merrill through in vitro culture is described. High frequency bud break (85%) and multiple shoot formation were induced from nodal segments explanted between September through November and cultured on MS medium supplemented with 2.0mg/l BAP. Although callus- free multiple shoot formation was a function of cytokinin activity alone, faster bud break coupled with enhanced frequency of shoot development (95%) and internode elongation were dependent on the synergistic effect of GA 3 (0.2mg/l). By repeated sub culturing of nodal segments harvested from the newly formed axenic shoots, prolific shoot cultures, free of proximal callusing, showing a high frequency multiplication rate were established within three months. The percentage shoot multiplication as well as the number of shoots per node attained the highest values (100%, 7 shoots/node) during the first two culture passages; beyond this there was a gradual decline in shoot bud differentiation. Rooting of the excised shoots from secondary or subsequent cultures was best induced on ½ strength MS medium containing 0.5 mg/l IBA. Vermi-compost was the most suitable planting substrate for hardening and its use ensured high frequency survival (96%) of regenerated plantlets prior to outdoor transfer. Regenerated plants get established in pots containing garden soil followed by their transfer to natural soil under full sun. The in vitro regenerated plants were uniform and identical in growth characteristics and morphology to the donor plants. (Journal of American Science 2010;6(12):385-392). (ISSN: 1545-1003).
Strain-mediated converse magnetoelectric coupling strength manipulation by a thin titanium layer

The manipulation of the strain-mediated magnetoelectric (ME) coupling strength is investigated by inserting a thin Ti layer (0–10 nm) between a 50 nm Co50Fe50 layer and a (011) oriented lead magnesium niobate-lead titanate (PMN-PT) substrate. A record high remanence ratio (Mr/Ms) tunability of 100% has been demonstrated in the 50 nm CoFe/8 nm Ti/PMN-PT heterostructure, when a total in-plane piezoelectric strain of −1821 ppm was applied at an electric field (E-field) of 16 kV/cm. The ME coupling strength is gradually optimized as the Ti layer thickness increases. Magnetic energy calculation showed that with increasing Ti layer thickness the uniaxial magnetic anisotropy energy (Euni) was reduced from 43 ± 1 kJ/m3 to 29.8 ± 1 kJ/m3. The reduction of Euni makes the strain effect dominant in the total magnetic energy, thus gives an obvious enhanced ME coupling strength.
NGA-West2 Equations for Predicting PGA, PGV, and 5% Damped PSA for Shallow Crustal Earthquakes

We provide ground motion prediction equations for computing medians and standard deviations of average horizontal component intensity measures (IMs) for shallow crustal earthquakes in active tectonic regions. The equations were derived from a global database with M 3.0–7.9 events. We derived equations for the primary M- and distance-dependence of the IMs after fixing the VS30-based nonlinear site term from a parallel NGA-West2 study. We then evaluated additional effects using mixed effects residuals analysis, which revealed no trends with source depth over the M range of interest, indistinct Class 1 and 2 event IMs, and basin depth effects that increase and decrease long-period IMs for depths larger and smaller, respectively, than means from regional VS30-depth relations. Our aleatory variability model captures decreasing between-event variability with M, as well as within-event variability that increases or decreases with M depending on period, increases with distance, and decreases for soft sites.
Improving processing by adaption to conditional geostatistical simulation of block compositions

One of the key usages of geostatistics has long been the prediction of the spatial structure of orebodies. This is used for the evaluation of resources and/or reserves and for further planning of the mining and beneficiation process schedule. For most applications, and until quite recently, metal grade has been regarded as the central property of study and the main objective has been to distinguish between ore and waste. However, recently other properties have come into focus through better analytical methods, such as automated mineralogy (see e.g. Fandrich et al. 2007), and new geostatistical methods considering more complex information, such as kriging of compositions (Pawlowsky-Glahn and Olea, 2004). Two ores with the same chemical composition can have totally different mineralogies and microfabrics, which will result in different recoveries, energy requirements, or reagent consumptions, thus yielding very different mass streams. An ore is thus no longer understood as represented by a single value element, but through a complex microfabric (Hagni, 2008). This perspective allows quantitative insight into relevant properties of different ore and gangue minerals (Sutherland and Gottlieb, 1991), potentially containing poison elements or phases (e.g. Houot, 1983) that modify the efficiency of downstream processing steps or require additional treatment. Accordingly, processing choices have become more complex. Grade-based studies allow a mere ‘beneficiate-or-dump’ decision. With the advent of these analytical and methodological advances, it is possible to better adapt processing to the ore mined due to a more profound understanding of the processing required. For instance, good knowledge of microfabric properties can reduce energy consumption, if overgrinding is avoided, which also results in improved recovery by avoiding losses due to poor liberation (Wills and Napier-Munn, 2006). Similarly, accurate information on mineral composition may permit the specification of a cut-off in any separation technique (magnetic, electrostatic, or density-based), optimally weighting recovery and further processing costs. Or depending on the proportion of fines generated during milling, desliming might or might not be necessary. Finally, different concentrations of chemicals might be needed for an optimal flotation process as a function of the composition of the concentrate. These Improving processing by adaption to conditional geostatistical simulation of block compositions