Miracle-dz/UniFAR-FTUs huggingface dataset:
Endolysosomal Dysfunction in Parkinson Disease: The Role of Disease Mutations in ATP13A2 and LRRK2.

Accumulating evidence from genetic and biochemical studies implicate dysfunction of the autophagic-lysosomal pathway as a key feature in the pathogenesis of Parkinson disease (PD). Most studies have focused on accumulation of neurotoxic α-synuclein secondary to defects in autophagy as the cause of neurodegeneration, but abnormalities of the autophagic-lysosomal system likely mediate toxicity through multiple mechanisms. To further explore how endolysosomal dysfunction causes PD-related neurodegeneration, I generated a murine model of Kufor-Rakeb syndrome (KRS), characterized by early-onset Parkinsonism with additional neurological features. KRS is caused by recessive loss-of-function mutations in the ATP13A2 gene encoding the late endosomal/lysosomal ATPase ATP13A2. I show that Atp13a2 null mice develop age-related motor abnormalities that are preceded by neuropathological changes including gliosis, accumulation of ubiquitinated protein aggregates, lipofuscinosis, and lysosomal abnormalities. Contrary to predictions from in vitro data, in vivo mouse genetic 1 This chapter was submitted for publication, in combination with part of Chapter 3, as Kett LR, Bernath M, Stiller B, Tasset I, Blesa J, Jackson-Lewis V, Chan RB, Di Paolo G, Przedborski S, Cuervo AM, Dauer WT. α-SynucleinIndependent Histopathological and Motor Deficits in Mice Lacking the Lysosomal Parkinsonism Protein Atp13a2.
SoftSystem: Smart Edge Computing Device Selection Method for IoT Based on Soft Set Technique

The Internet of Things (IoT) is growing day by day, and new IoT devices are introduced and interconnected. Due to this rapid growth, IoT faces several issues related to communication in the edge computing network. The critical issue in these networks is the effective edge computing IoT device selection whenever there are several edge nodes to carry information. To overcome this problem, in this paper, we proposed a new framework model named SoftSystem based on the soft set technique that recommends useful IIoT devices. Then, we proposed an algorithm named Softsystemalgo. For the proposed system, three different parameters are selected: IoT Device Security (IDSC), IoT Device Storage (IDST), and IoT Device Communication Speed (IDCS). We also find out the most significant parameters from the given set of parameters. It is evident that our proposed system is effective for the selection of edge computing devices in the IoT network.
INFLUENCE OF PROCESS PARAMETERS ON THE FRICTION COEFFICIENT IN ONE AND MULTI PHASE STEEL STRIP DRAWING IRONING TEST

Part of the experimental results of influence on friction in the stripe ironing process with double thinning are presented in this paper. Applied was Schlosser model for evaluating influence of lateral force, contact pressure, average absolute roughness height and thinning strain on friction coefficient. Applied was classical model because of sufficient intensity of drawing and lateral force. If a lateral and drawing forces are small, classical model is not suitable and give unreal negative friction coefficient values. 20 mm wide and 2.5 mm thick strips of mild steel DC04 sheets were used in the single, three and four-phase process with a maximum thinning deformation of about 29%. Appropriate lubricant, mineral oil, was used in conditions of lower speed of 20 mm/min. Three and four phase process was realized with variable lateral force of 5, 10, 15 and 20 kN. The applied experimental test procedure enables the precise quantification of lateral force, contact pressure, thinning strain and roughness influence on friction to be established. Test also enables evaluation of lubricants quality.
A whole genome analysis of the red-crowned crane provides insight into avian longevity

The red-crowned crane (Grus japonensis) is an endangered and large-bodied crane native to East Asia. It is a traditional symbol of longevity and its long lifespan has been confirmed both in captivity and in the wild. Lifespan in birds is positively correlated with body size and negatively correlated with metabolic rate; although the genetic mechanisms for the red-crowned crane’s long lifespan have not previously been investigated. Using whole genome sequencing and comparative evolutionary analyses against the grey-crowned crane and other avian genomes, we identified candidate genes that are correlated with longevity. Included among these are positively selected genes with known associations with longevity in metabolism and immunity pathways (NDUFA5, NDUFA8, NUDT12 IL9R, SOD3, NUDT12, PNLIP, CTH, and RPA1). Our analyses provide genetic evidence for low metabolic rate and longevity, accompanied by possible convergent adaptation signatures among distantly related large and long-lived birds. Finally, we identified low genetic diversity in the red-crowned crane, consistent with its listing as an endangered species, and we hope this genome will provide a useful genetic resource for future conservation studies of this rare and iconic species.
HYBRID SWARM OPTIMIZATION APPROACHES: A SURVEY

-Data is continuously increasing due to millions of transactions done daily by the users. A plenty of classification techniques are available for discovering useful information out of this huge amount of data. Now-a-days swarm optimization approaches and the hybridization of these approaches are playing important role for classification in highly efficient manner. In this paper, we provide an overview and a detailed comparison of some of the swarm optimization approaches and hybrid swarm optimization approaches present in research literature.
The mathematical model of postoperative mortality in cases of duodenal injuries: A single center review of a 14-year experience

Objective: Traumatic injuries of the duodenum are quite rare, often accompanied by local and systemic postoperative complications, as well as high mortality. Therefore, identifying predictors of early death in this category of patients is an urgent problem. Materials and methods: A retrospective study included 72 patients older than 18 years old with traumatic injuries of the duodenum ≥ II degree, according to the criteria of the American Association of Surgery for Trauma, who were operated from 2006 to 2020. Patients were assigned into two groups: the 1 group (survivors, n=58) and the 2 group (non-survivors, n=14). Different methods of statistical analysis were used to construction the mathematical model. Results: Multiple injuries of intra-abdominal and anatomical structures of the retroperitoneal space were in 72.2% patients. Mean RTS, ISS, and APACHE II scores in the deceased and the surviving patients were and 6.89±1.0 and 3.93±0.7; and 12.36±3.9 and 20.6±4.5; 14.05±3.2 and 26.67±4.1, respectively (p=0.000). All indicators were identified whose changes were most different in surviving and deceased patients on admission to the hospital: blood RESEARCH 24(104), July August, 2020 Medical Science ISSN 2321–7359 EISSN 2321–7367
Hemagglutinin frommultiple divergent influenza A and B viruses bind to a distinct branched, sialylated poly-LacNAc glycan by surface plasmon resonance

https://doi.org/10.1016/j.vaccine. 2020.08. 037 0264-410X/ 2020 Novavax, Inc. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). ⇑ Corresponding author. E-mail addresses: pbruce@biofactura.com (P.J. Bruce-Staskal), robert.woods@catalent.com (R.M. Woods), oborisov@novavax.com (O.V. Borisov), mmassare@novavax.com (M.J. Massare), thahn@novavax.com (T.J. Hahn). 1 PJBS and RMW each contributed comparably as co-lead authors. 2 Present address. Pamela J. Bruce-Staskal , Robert M. Woods , Oleg V. Borisov , Michael J. Massare , Timothy J. Hahn c,⇑
Emerging role of ONECUT2

One cut domain family member 2 (ONECUT2), also termed OC‐2, is a newly discovered member of the ONECUT transcription factor family. As a transcription factor, ONECUT2 can widely regulate protein expression associated with cell proliferation, migration, adhesion, differ‐ entiation and cell material metabolism. Recent studies have revealed that ONECUT2 is associated with tumor cell proliferation, angiogenesis and metastasis; it is also associ‐ ated with epithelial‐mesenchymal transition in cancer cells. The present review examines the distribution and expression of ONECUT2 in a variety of tumors, its oncogenic role in tumor progression and the possible mechanisms of regulation. Given the emerging role of ONECUT2 in the development and progression of tumors, ONECUT2 might be a promising target for tumor therapy.
Effect of Pre-Treatments on Biochemical and Microbial Parameters of Guava Fruit during storage

Freshly harvested and fully matured guava fruits (Lucknow-49) were hydro-cooled at 2 ± 1°C for 10 min and pretreated with different treatments viz., calcium chloride (2 %), hydrogen peroxide (1 %), benomyl (0.1 %), neem oil (2 %), lemon grass oil (0.2 %), cinnamon oil (4 %) and ozone (150 mg/h). Fruits were packed in 50 μm LDPE bags and stored at 10 ± 1 o C. Control fruits without any pre-treatment were stored at low as well as room temperature. The biochemical, sensory and microbial parameters of the guava fruit were recorded at 5 days interval during storage. Minimum TSS (14.1 o Brix) and total sugar (8.94 %) were observed in ozone treatment, while maximum titratable acidity (1.13 %) and ascorbic acid (236 mg/100 g) was observed in ozone treatment on 30 days of storage. Maximum sensory score was found in ozone and in cinnamon oil treatments. Microbial parameters viz., total plate count, E.coli, salmonella and yeast and mould in the fruit were found absent for ozone, cinnamon oil, neem oil and hydrogen peroxide treatments. Maximum changes in biochemical, sensory and microbial parameters were found in control at room temperature followed by control at low temperature. Shelf life of guava fruit could be increased up to 30 days with minimum changes in biochemical, sensory and microbial parameters. when the fruits pretreated with ozone (150 mg/h) followed by packaging in 50 μm LDPE bags at 10 ± 1 o C storage temperature.
Nonresponse Bias Reduction Integrating Calibration Constraints with Variance and Covariance Structure of Auxiliary Variables

Riassunto: Per ridurre gli effetti distorsivi nelle stime finali causati dalle mancate risposte totali, Lundström e Särndal (1999) suggeriscono d’impiegare lo stimatore di calibrazione come strumento prevalente nella fase di stima. Tuttavia, se il campione dei rispondenti differisce sistematicamente dai non rispondenti, la procedura di modifica dei pesi campionari, indicata da questi autori, non corregge a sufficienza l’effetto distorsivo sulle stime, dovuto all’autoselezione dei rispondenti. In questo articolo si propone una modifica dello stimatore di calibrazione che incorpori, tra i vincoli da soddisfare, anche la struttura di varianza e covarianza conosciuta delle variabili ausiliarie. Tale variante sembra attenuare più efficacemente la distorsione delle stime e può essere considerata una valida alternativa, quando risulta arduo modellizzare la mancata risposta.
Genetic diversity of Bulgarian representatives of genus Carduus L. (Asteraceae) as revealed by variability in sequences of internal transcribed spacers region

ABSTRACT The biodiversity of genus Carduus in Bulgaria is grossly under-studied with modern methods, which leads to unclear status of species and hampers the assessment of their habitats and conservation status. In this study, we used the variability of internal transcribed spacers (ITS) to investigate the biodiversity of Carduus species collected from different floristic regions of Bulgaria. Thirty-three samples were processed. Among the studied species, C. acanthoides exhibited the highest ITS variability (eight single nucleotide polymorphisms were found). High ITS variability was also found in C. crispus and C. hamulosus, demonstrating that some of the local Carduus species possesses unique genetic diversity, which can serve as source for future divergence of new forms and subspecies. For each species, unique nucleotides suitable as molecular taxonomy markers were identified. However, C. nutans and C. thoermeri displayed completely identical ITS sequences, which is in agreement with all ITS sequences of these species collected elsewhere in the world and deposited in the NCBI database. After careful analyses of both molecular and morphological data, we propose to restore C. nutans and C. thoermeri as one species with two subspecies. Namely, Carduus nutans subsp. nutans and Carduus nutans subsp. thoermeri. We recommend diameter of capitulum, length of peduncle, width of peduncle and width of bract as diagnostic features of taxonomic significance for distinguishing the two subspecies.
Informal Sector Economy, Child Labor and Economic Growth in Developing Economies: Exploring the Interlinkages

The study explores the interlinkages among informal sector, child labor and economic growth covering 50 developing economies for the time period 2001-2015 through 3SLS approach. The results show that informal sector positively influences child labor and child labor positively influences informal sector. It creates a vicious cycle of underdevelopment and poverty. Similarly child labor positively contributes in economic growth and economic growth enhances child labor in developing economies. However, informal sector increases economic growth but economic growth decreases informal sector. So there emerged a complex and discoursing interlinkage among informal sector, child labor and economic growth in developing economies. The informal sector is positively influenced by population growth. Child labor is also positively influenced by population growth and age dependency but negatively by globalization. Economic growth is positively influenced by capital formation, globalization and governance. A multidimensional approach is required for decreasing the size of informal sector economy and eliminating the child labor along with having good economic growth. Control of population growth is emerged one of them which may slide down informal sector economy and child labor. The capital formation and governance may be a part of the policy framework for economic growth.
Issues in Information Systems

Internet of Things (IoT) has become one of the cutting-edge technologies and an attractive area of interest for the research world, and economically attractive for the business world. It involves the interconnection of multiple devices and connections of devices to humans. IoT requires a cloud computing environment to handle its data exchange and processing. At the same time, it requires artificial intelligence (AI) to analyze the data stored at cloud infrastructure and make fast and reliable intelligent decisions. These interconnected IoT devices use their unique-identifiers and the embedded sensor with each device to communicate with each other, and exchange information among them using an Internet and cloud-based network infrastructure (Girma, 2018). We are living in the era of big data where the necessity of applying AI/ML has been very critical to the process and analyze the collected cloud-based big data fast and accurately. However, even though AI is currently playing a vital role in improving the traditional cybersecurity, both the cloud vulnerability and the networking of IoT devices are still major threats. Besides the security issues of cloud computing, IoT devices, and AI is being used by hackers and continues to be a threat to the world of cybersecurity. Moreover, most of wirelessly accessed IoT devices deployed on a public network are also under constant cyber threats. This research paper will propose a hybrid detection model as a solution approach using artificial intelligence and machine learning (AI/ML) to combat and mitigate IoT cyber threats on cloud computing environments both at the host-based and network level. KEYWORDSIoT security; cyber threats, Cloud Computing, artificial intelligence, machine learning
Feature Selection in Session Matrix Using Enhanced ICA

-User behavior analysis helps the visitors browsing work more effective and simple and is an important research area in this internet scenario. It is used to analyze a new user and study their present behavior and classify them in a group of similar users. The group consists of user navigation patterns which are used for many applications like personalization, site improvement, business intelligence, and web page recommender system. The navigation pattern consists of features which are both relevant and irrelevant for mining process. To improve the overall clustering and classification results only important features have to be discovered in user navigation matrix. This work analyzes each and every feature and selects the relevant and important features for mining by using enhanced Independent Component Analysis. The experimental results shows that the proposed method selects more relevant features in lesser time.
TOPOLOGICAL DERIVATIVE FORMULATION FOR SHAPE SENSITIVITY IN INCOMPRESSIBLE TURBULENT FLOWS

ABSTRACT Shape derivative based on topological consideration is presented in this work. The resulting derivative has simpler form compared to corresponding classical shape derivative due to topological derivative formulation. Shape derivative is based on topological derivative in the limit of infinitesimally weak source terms in momentum equation approaching the boundary of the computational domain. The consistency of two derivatives is demonstrated and computational example of the flow in curved duct is used for the illustration of the derivative computations.
Theoretical study of [1,5]-hydrogen shift in substituted 1,8a- dihydronaphenthalene

The [1,5]-hydrogen shift stimulated many mechanistic studies [1.2] and has found numerous applications in organic synthesis[3,4]. This reaction usually proceeds by concerted pathway involving an aromatic transition state[5,6,7]. The effect of aromatic stabilization on the rates of [1,5]- hydrogen shift were previously estimated experimentally and theoretically. Not only the activation energy but also the shape and the position of the reaction barrier can be efficiently controlled by change in aromaticity.
Crystal structure of the MBD domain of MBD3 in complex with methylated CG DNA

MBD3 is a core subunit of the Mi-2/NuRD complex, and has been previously reported to lack methyl-CpG binding ability. However, recent reports show that MBD3 recognizes both mCG and hmCG DNA with a preference for hmCG, and is required for the normal expression of hmCG marked genes in ES cells. Nevertheless, it is not clear how MBD3 recognizes the methylated DNA. In this study, we carried out structural analysis coupled with isothermal titration calorimetry (ITC) binding assay and mutagenesis studies to address the structural basis for the mCG DNA binding ability of the MBD3 MBD domain. We found that the MBD3 MBD domain prefers binding mCG over hmCG through the conserved arginine fingers, and this MBD domain as well as other mCG binding MBD domains can recognize the mCG duplex without orientation selectivity. Furthermore, we found that the tyrosine-to-phenylalanine substitution at Phe34 of MBD3 is responsible for its weaker mCG DNA binding ability compared to other mCG binding MBD domains. In summary, our study demonstrates that the MBD3 MBD domain is a mCG binder, and also illustrates its binding mechanism to the methylated CG DNA.
Analysis of Space Manipulation in an Informal Urban Settlement: The Case of Ng’ombe in Lusaka, Zambia

Urban informality remains a consistent challenge and matter of debate by planners and policymakers in the urbanising cities of sub-Saharan Africa. A common manifestation of urban informality in African cities is the sprawling informal settlements that constitute the only available housing option for the majority of the urban poor. The analysis of informal urban settlement’s environmental composition, physical modelling and socio-economic and policy analysis have been areas of recent study. However there is limited literature on how the urban poor communities in Zambia manipulate their social, spatial and economic environments to meet their needs. This article seeks to broaden the knowledge base on the way informal urban settlement communities manipulate their urban space. The built environment provides the setting for human interaction and the explanatory theory of Environment-Behaviour Relations provides a suitable analytical framework for the identification of useful parameters for developing future settlement interventions. The study employs a case study method of research to analyse the informal urban settlement settings. Ng’ombe, a peri-urban informal settlement in Lusaka, Zambia is the case study location for this research. Analysis of social, spatial and economic environment at neighbourhood level provides vital information about the informal urban settlement conditions. The study in particular addresses the question of how the social-spatial circumstances of the informal urban population in the developing world influence and defines their built environment. The study shows that systems of settings and system of activities in Ng’ombe offer a suitable analytical framework for studying the settlement characteristics that can guide in formulating strategies for settlement regularisation. Residents devise means of adapting and manipulating their informal urban space to suit their immediate needs and they also devise livelihood coping strategies in the midst of their informal settings. The paper likewise contributes to the growing body of knowledge in urban informality.
Estimation of total antioxidant capacity of milk of Malabari goat and vechur cattle during different lactation

The present study was conducted to evaluate the changes in the antioxidant level of milk in different phases of lactation in Malabari goat and Vechur cattle, the native breeds of Kerala. Milk samples from 6 animals each of Malabari goats and Vechur cattles were collected separately at early, mid and late lactation period. Total antioxidant capacity was estimated by using ferric reducing antioxidant power. The study revealed that, irrespective of species the antioxidant capacity of milk at early lactation was higher than that at mid and late lactation. Throughout the lactation period the antioxidant capacity of Vechur milk was higher than that of goat milk (p<0.01). The study further indicates that, irrespective of the species milk during the early lactation contained significantly higher content of antioxidants than the other two stages of lactation. High antioxidant capacity of milk of Vechur cattle is suggestive of high medicinal value which may be due to abundant presence of bioactive peptides.
The Evolution of Extended Sexual Receptivity in Chimpanzees: Variation, Male-Female Associations, and Hormonal Correlates

The Evolution of Extended Sexual Receptivity in Chimpanzees: Variation, Male-Female Associations, and Hormonal Correlates by Emily Elizabeth Blankinship Boehm Department of Evolutionary Anthropology Duke University Date:____________________ Approved: ___________________________ Anne Pusey, Supervisor ___________________________ Susan Alberts ___________________________ Christine Drea ___________________________ Stephen Nowicki ___________________________ Charles Nunn ___________________________ Christine Wall An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Evolutionary Anthropology in the Graduate School of Duke University