Miracle-dz/UniFAR-FTUs huggingface dataset:
Paternal trans‐fatty acid and vitamin E diet affects rat offspring's semen quality and PPARs expression

Trans‐fatty acids (TFAs) consumption has created concerns regarding male/female reproductive system. However, the effects of TFA in paternal diet on offspring's reproduction have not been addressed. The purpose of this study is to investigate the effects of rat paternal TFAs and vitamin E consumption on offspring's sperm quality and expression pattern of peroxisome proliferator‐activated receptors (PPARs) in testis tissues. Forty adult male rats were randomly divided into four groups: Control diet (C); Control diet plus TFA (CTH); diet supplemented with vitamin E (E) and a diet containing vitamin E and TFA (ETH). Mother rats had normal diet during gestation period. Three offspring from each group were chosen randomly and their testicular samples were collected, and sperm parameters were measured by CASA. Our results indicate that feeding fathers with TFA can negatively affect offspring's sperm concentration and motility, while consumption of vitamin E can improve these parameters (p < .05). The paternal diet containing TFA down‐regulated the expression of PPARβ and PPARγ genes, whereas vitamin E‐containing diet up‐regulated the transcription of PPAR genes. In conclusion, TFA intake in paternal diet may have negative effects on reproductive system of the offspring while vitamin E may not diminish these effects.
Characteristics of adolescents aged 15-19 years living with vertically and horizontally acquired HIV in Nampula, Mozambique

Background Adolescents living with HIV (ALHIV) 15–19 years of age are a growing proportion of all people living with HIV globally and the population includes adolescents with vertically acquired HIV (AVH) and behaviorally acquired HIV (ABH). Methods We conducted a survey to measure sociodemographic characteristics, educational status, health history, and antiretroviral therapy (ART) adherence among a convenience sample of ALHIV at three government health facilities in 2019 in Nampula, Mozambique. ALHIV 15–19 years on ART, including females attending antenatal care, were eligible. Routine HIV care data were extracted from medical charts. Classification of ALHIV by mode of transmission was based on medical charts and survey data. ALHIV who initiated ART <15 years or reported no sex were considered AVH; all others ABH. Frequencies were compared by sex, and within sex, by mode of transmission (AVH vs. ABH) using Chi-square, Fishers exact tests and Wilcoxon rank-sum tests. Results Among 208 ALHIV, 143 (69%) were female and median age was 18 years [interquartile range (IQR) 16–19]. Just over half of ALHIV (53%) were in or had completed secondary or higher levels of education; the most common reason for not being in school reported by 36% of females was pregnancy or having a child. Of all ALHIV, 122 (59%) had VL data, 62% of whom were <1000 copies/mL. Almost half (46%) of ALHIV reported missing ARVs ≥ 1 day in the past month (62% of males vs. 39% of females; p = 0.003). Just over half (58%) of ALHIV in relationships had disclosed their HIV status: 13% of males vs. 69% of females (p<0.001). Among sexually active males, 61% reported using a condom at last sex compared to 26% of females (p<0.001). Among female ALHIV, 50 (35%) were AVH and 93 (65%) were ABH, 67% of whom were not in school compared to 16% of ABH, (p<0.001). Discussion Data from our study underscore the high level of deprivation among ALHIV enrolled in HIV care in Mozambique, as well as important disparities by sex and mode of transmission. These data can inform the development of effective interventions for this complex and important population.
Photopolymerizable, Universal Antimicrobial Coating to Produce High-Performing, Multifunctional Face Masks

COVID-19 poses a major threat to global health and socioeconomic structures, and the need for a highly effective, antimicrobial face mask has been considered a major challenge for protection against respiratory diseases. Here, we report the development of a universal, antiviral, and antibacterial material that can be dip-/spray-coated over conventional mask fabrics to exhibit antimicrobial activities. Our data shows that antimicrobial fabrics rapidly inactivated multiple types of viruses, i.e., human (alpha/beta) coronaviruses, the influenza virus, and bacteria, irrespective of their modes of transmission (aerosol or droplet). This research provides an immediate method to contain infectious diseases, such as COVID-19.
Carrier statistics in graphene at high electric field

The saturated velocity of a semiconductor is an important measure of performance. Graphene has been of interest for many applications due to its apparently high value of the saturated velocity. Recent experiments have suggested that this value is very density dependent and can even exceed the band limiting Fermi velocity. On the other hand, recent calculations have shown that the saturated velocity is largely independent of the carrier density. Here, we show that the carrier heating, due to high electric fields, can change the carrier statistics. This, and the fact that minority carriers can significantly impact the conductance may lead to erroneous conclusions about the actual total carrier density in the experimental situation.
Moisture Adsorption Isotherms and Thermodynamic Properties of Penaeus vannamei Meat with and without Maltodextrin Addition

ABSTRACT Moisture adsorption isotherms of freeze-dried Penaeus vannamei meat containing maltodextrin (PV-MD) and maltodextrin-free (PV) samples were determined at temperatures of 5, 15, 25, 35, and 45°C with water activity (aw) ranging from 0.112 to 0.931. The adsorption behaviors of PV and PV-MD followed type III isotherms. The equilibrium moisture content of PV and PV-MD decreased with increasing temperature and increased with increasing aw. The monolayer moisture content increased with the addition of maltodextrin and was observed to be 0.0611–0.0824 and 0.0734–0.1034 g water/g dry solids for PV and PV-MD, respectively. Eight models were employed to fit experimental data, and the Guggenheim-Anderson-de Boer (GAB) model achieved the best fit. For both PV and PV-MD, the differential enthalpy and entropy and integral enthalpy decreased with increasing moisture content. The integral entropy was negative in value and increased with increasing moisture content. The spreading pressure increased with increasing aw but decreased with increasing temperature. A plot of differential enthalpy versus entropy satisfied the enthalpy-entropy compensation theory, and the adsorption behavior of P. vannamei meat was enthalpy-driven and spontaneous. Addition of maltodextrin increased the differential enthalpy and entropy, integral enthalpy, and spreading pressure, but decreased the integral entropy of P. vannamei meat.
Lumbo-pelvic proprioception in sitting is impaired in subgroups of low back pain–But the clinical utility of the differences is unclear. A systematic review and meta-analysis

Background Altered spinal postures and altered motor control observed among people with non-specific low back pain have been associated with abnormal processing of sensory inputs. Evidence indicates that patients with non-specific low back pain have impaired lumbo-pelvic proprioceptive acuity compared to asymptomatic individuals. Objective To systematically review seated lumbo-pelvic proprioception among people with non-specific low back pain. Methods Five electronic databases were searched to identify studies comparing lumbo-pelvic proprioception using active repositioning accuracy in sitting posture in individuals with and without non-specific low back pain. Study quality was assessed by using a modified Downs and Black’s checklist. Risk of bias was assessed using an adapted tool for cross-sectional design and case–control studies. We performed meta-analysis using a random effects model. Meta-analyses included subgroup analyses according to disability level, directional subgrouping pattern, and availability of vision during testing. We rated the quality of evidence using the GRADE approach. Results 16 studies met the eligibility criteria. Pooled meta-analyses were possible for absolute error, variable error, and constant error, measured in sagittal and transverse planes. There is very low and low certainty evidence of greater absolute and variable repositioning error in seated tasks among non-specific low back pain patients overall compared to asymptomatic individuals (sagittal plane). Subgroup analyses indicate moderate certainty evidence of greater absolute and variable error in seated tasks among directional subgroups of adults with non-specific low back pain, along with weaker evidence (low-very low certainty) of greater constant error. Discussion Lumbo-pelvic proprioception is impaired among people with non-specific low back pain. However, the low certainty of evidence, the small magnitude of error observed and the calculated “noise” of proprioception measures, suggest that any observed differences in lumbo-pelvic proprioception may be of limited clinical utility. PROSPERO-ID CRD42018107671
Statin Effect on Coronary Calcium Distribution, Mass and Volume Scores and Associations with Immune Activation among HIV+ Persons on Antiretroviral Therapy

Background Inflammation has been associated with whole heart coronary artery calcification (CAC) among people with HIV (PWH) on antiretroviral therapy (ART); however, prior studies have not evaluated the distribution of calcium or separated mass versus volume scores, which are differentially associated with clinical events in the general population. Statins may also have a greater effect on CAC mass compared with volume. Methods 147 PWH were randomized 1:1 to rosuvastatin 10 mg or placebo and followed for 96 weeks. We re-analysed coronary calcium scans from 0, 48 and 96 weeks to determine mass and volume scores and measures of CAC diffusivity. Mixed effects models and generalized estimating equations were used to examine longitudinal associations of CAC with treatment and biomarkers. Results Median age at study entry was 46 years; 78% were male and 68% African American. Median CD4+ was 613 and half were on protease inhibitors. Randomization to statin therapy was not associated with a change in mass score, volume score, number of involved vessels or diffusivity index (all P>0.1). Soluble CD14 was associated with the presence of CAC (P=0.05) and borderline associated with number of involved vessels (P=0.07) across all three time points. Conclusions In PWH on ART, moderate intensity rosuvastatin does not appear to have a significant effect on volume, mass or regional distribution of CAC over 96 weeks. We extend previous cross-sectional observations to show that soluble CD14 is associated with whole heart CAC over time and independently of age and systolic blood pressure.
Fast but not furious: a streamlined selection method for genome-edited cells

Magnetic-activated genome-edited cell sorting (MAGECS) allows a fast and easy generation of genetically modified cells. In the last decade, transcription activator-like effector nucleases and CRISPR-based genome engineering have revolutionized our approach to biology. Because of their high efficiency and ease of use, the development of custom knock-out and knock-in animal or cell models is now within reach for almost every laboratory. Nonetheless, the generation of genetically modified cells often requires a selection step, usually achieved by antibiotics or fluorescent markers. The choice of the selection marker is based on the available laboratory resources, such as cell types, and parameters such as time and cost should also be taken into consideration. Here, we present a new and fast strategy called magnetic-activated genome-edited cell sorting, to select genetically modified cells based on the ability to magnetically sort surface antigens (i.e., tCD19) present in Cas9-positive cells. By using magnetic-activated genome-edited cell sorting, we successfully generated and isolated genetically modified human-induced pluripotent stem cells, primary human fibroblasts, SH-SY5Y neuroblast-like cells, HaCaT and HEK 293T cells. Our strategy expands the genome editing toolbox by offering a fast, cheap, and an easy to use alternative to the available selection methods.
Behind the scenes: Are latent memories supported by calcium independent plasticity?

N‐methyl‐D‐aspartate receptors (NMDARs) can be considered to be the de facto “plasticity” receptors in the brain due to their central role in the activity‐dependent modification of neuronal morphology and synaptic transmission. Since the 1980s, research on NMDARs has focused on the second messenger properties of calcium and the downstream signaling pathways that mediate alterations in neural form and function. Recently, NMDARs were shown to drive activity‐dependent synaptic plasticity without calcium influx. How this “nonionotropic” plasticity occurs in vitro is becoming clearer, but research on its involvement in behavior and cognition is in its infancy. There is a partial overlap in the downstream signaling molecules that are involved in ionotropic and nonionotropic NMDAR‐dependent plasticity. Given this, and prior studies of the cognitive impacts of ionotropic NMDAR plasticity, a preliminary model explaining how NMDAR nonionotropic plasticity affects learning and memory can be established. We hypothesize that nonionotropic NMDAR plasticity takes part in latent memory encoding in immature rodents through nonassociative depression of synaptic efficacy, and possibly shrinking of dendritic spines. Further, the late postnatal alteration in NMDAR composition in the hippocampus appears to reduce nonionotropic signaling and remove a restriction on memory retrieval. This framework substantially alters the canonical model of NMDAR involvement in spatial cognition and hippocampal maturation and provides novel and exciting inroads for future studies.
Exposure to diverse Plasmodium falciparum genotypes shapes the risk of symptomatic malaria in incident and persistent infections: A longitudinal molecular epidemiologic study in Kenya.

BACKGROUND Repeated exposure to malaria infections could protect against symptomatic progression, as people develop adaptive immunity to infections acquired over time. METHODS We investigated how new, recurrent, and persistent Plasmodium falciparum infections were associated with the odds of developing symptomatic compared to asymptomatic malaria. Using a 14-month longitudinal cohort in Western Kenya, we used amplicon deep sequencing of two polymorphic genes (pfama1 and pfcsp) to assess overlap of parasite genotypes (represented by haplotypes) acquired within an individual's successive infections. We hypothesized infections with novel haplotypes would increase the odds of symptomatic malaria. RESULTS After excluding initial infections, we observed 534 asymptomatic and 88 symptomatic infections across 186 people. We detected 109 pfcsp haplotypes, and each infection was classified as harboring novel, recurrent or persistent haplotypes. Incident infections with only new haplotypes had higher odds of symptomatic malaria when compared to infections with only recurrent haplotypes [odds ratio (OR): 3.24, 95% confidence interval (CI): 1.20 to 8.78], but infections with both new and recurrent haplotypes [OR: 0.64, 95% CI: 0.15 to 2.65] did not. Assessing persistent infections, those with mixed (persistent with new or recurrent) haplotypes [OR: 0.77, 95% CI: 0.21 to 2.75] had no association with symptomatic malaria compared to infections with only persistent haplotypes. Results were similar for pfama1. CONCLUSIONS These results confirm that incident infections with only novel haplotypes were associated with increased odds of symptomatic malaria compared to infections with only recurrent haplotypes but this relationship was not seen when haplotypes persisted over time in consecutive infections.
A new species of planthopper in the genus Haplaxius/ (Hemiptera: Auchenorrhyncha: Fulgoroidea: Cixiidae) from coconut palm (Cocos/ nucifera/) in Costa Rica.

Haplaxius is a large genus of cixiid planthoppers that is widespread in the New World and economically important due to the role of H. crudus in transmitting palm lethal decline phytoplasmas. A new species of Haplaxius, here described as Haplaxius pocococo sp. n. , was discovered during survey work on palms in north-central Costa Rica. Placement in Haplaxius is supported by sequence analysis of the COI and 18S genes relative to congeners and by morphological characters.
Variable Emergence of Autism Spectrum Disorder Symptoms From Childhood to Early Adulthood.

OBJECTIVE Autism spectrum disorder (ASD) is currently considered an early-onset neurodevelopmental condition. Follow-up studies of clinic-ascertained autism suggest that autistic symptoms typically decline with age, although symptom improvement is limited for some. To date there have been no population-based prospective studies investigating the natural history of autistic symptoms from childhood to adulthood. The aim of this study was to characterize the development and heterogeneity of autistic symptoms in a population-based cohort from childhood to age 25. METHODS Data were analyzed in a prospective U.K. population-based cohort (ALSPAC). Trajectories were derived using five assessments of the parent-rated Social and Communication Disorders Checklist (SCDC) spanning ages 7-25. Additional measures were used to validate symptom trajectories. RESULTS Three distinct SCDC symptom trajectory classes were identified: low (88.5%), declining (5.0%), and late-emerging (6.5%). Both the declining and late-emerging trajectory classes were associated with child and adult ASD measures, low IQ, communication problems, peer problems, and worse adult functioning compared with the low trajectory class. Male sex was associated with a higher likelihood of being in the declining trajectory class (odds ratio=2.84, 95% CI=2.19, 3.69). This sex difference was not observed in the late-emerging class (odds ratio=1.00, 95% CI=0.80, 1.24) compared with the low trajectory class. CONCLUSIONS ASD symptom levels that emerged early tended to decline across development, although impairment was still present in adulthood for some. For others, autistic symptoms emerged across adolescence and adulthood. This challenges our current understanding that ASD symptoms inevitably first manifest early in development.
A simple perylene diimide derivative with a highly twisted geometry as an electron acceptor for efficient organic solar cells

Perylene diimide (PDI), which features intense absorption, a low-lying energy level, and high electron mobility, is a promising building block for electron acceptors in organic solar cells (OSCs). However, this planar molecule has a strong tendency to form large aggregates during film formation which strongly limits its OSC performance. Herein, we report a new and simple PDI derivative, B(PDI)3, in which a central benzene unit is employed to connect three PDI arms. This compact arrangement of sterically bulky PDI moieties leads to a twisted molecular geometry of the resultant structure. This suppresses the strong crystallization tendency of PDI chromophores, owing to the broken molecular coplanarity and symmetry. Therefore, B(PDI)3 is applied as a non-fullerene acceptor in OSCs, providing a good power conversion efficiency of 5.65% when blended with the PTB7-Th donor.
Problem solving in European bison (Bison bonasus): two experimental approaches

The ability to solve novel problems is crucial for individual fitness. However, studies on problem solving are usually done on few taxa, with species with low encephalization quotient being rarely tested. Here, we aimed to study problem solving in a non-domesticated ungulate species, European bison, with two experimental tasks. In the first task, five individuals were presented with a hanging barrel filled with food, which could either be directly accessed (control condition) or which could only be reached by pushing a tree stump in the enclosure below it and stepping on it (experimental condition). In the second task, five individuals were repeatedly fed by an experimenter using a novel bucket to retrieve food from a bag. Then, three identical buckets were placed in the enclosure, while the experimenter waited outside with the bag without feeding the bison, either with a bucket (control condition) or without it (experimental condition). In the first task, no bison moved the stump behind the barrel and/or stepped on it to reach the food. In the second task, two individuals solved the task by pushing the bucket within the experimenter's reach, twice in the experimental and twice in the control condition. We suggest that bison showed a limited ability to solve novel problems, and discuss the implications for their understanding of the functional aspects of the tasks.
Performance-Portable Distributed k-Nearest Neighbors using Locality-Sensitive Hashing and SYCL

In the age of data collection, machine learning algorithms have to be able to efficiently cope with vast data sets. This requires scalable algorithms and efficient implementations that can cope with heterogeneous hardware. We propose a new, performance-portable implementation of a well-known, robust, and versatile multi-class classification method that supports multiple Graphics Processing Units (GPUs) from different vendors. It is based on a performance-portable implementation of the approximate k-nearest neighbors (k-NN) algorithm in SYCL. The k-NN assigns a class to a data point based on a majority vote of its neighborhood. The naive approach compares a data point x to all other data points in the training data to identify the k nearest ones. However, this has quadratic runtime and is infeasible for large data sets. Therefore, approximate variants have been developed. Such an algorithm is the Locality-Sensitive Hashing (LSH) algorithm, which uses hash tables together with locality-sensitive hash functions to reduce the data points that have to be examined to compute the k-NN. To the best of our knowledge, there is no distributed LSH version supporting multiple GPUs from different vendors available so far despite the fact that k-NNs are frequently employed. Therefore, we have developed the library. It provides the first hardware-independent, yet efficient and distributed implementation of the LSH algorithm that is suited for modern supercomputers. The implementation uses C++17 together with SYCL 1.2. 1, which is an abstraction layer for OpenCL that allows targeting different hardware with a single implementation. To support large data sets, we utilize multiple GPUs using the Message Passing Interface (MPI) to enable the usage of both shared and distributed memory systems. We have tested different parameter combinations for two locality-sensitive hash function implementations, which we compare. Our results show that our library can easily scale on multiple GPUs using both hash function types, achieving a nearly optimal parallel speedup of up to 7.6 on 8 GPUs. Furthermore, we demonstrate that the library supports different SYCL implementations—ComputeCpp, hipSYCL, and DPC++—to target different hardware architectures without significant performance differences.