Miracle-dz/UniFAR-FTUs huggingface dataset:
Conifer somatic embryogenesis: I. Development

The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed. Additional key words: cryopreservation, initiation, maturation, Pinaceae, somatic seedlings Addresses: K. Klimaszewska1, Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E. P.S. , P.O. Box 3800, Sainte-Foy, Quebec, Canada G1V 4C7. D. R. Cyr, Orycle Consulting, 801-288 E 8th Ave., Vancouver, British Columbia, Canada V5T 4S8. 1 Corresponding author: e-mail: kklimaszewska@cfl.forestry. ca, tel. (418) 648-4638, fax (418) 648-5849
Landfarming of polycyclic aromatic hydrocarbons and mineral oil contaminated sediments

Important in this study was the landfarm on Kreekraksluizen where landfarming was investigated in cooperation with RIZA, during a period of more than 10 years, which made it possible to predict the effect of landfarming in the long term. The database for this site is unique and will be extended in the future. The large experimental research on Kreekraksluizen was supported by several studies at other sites and in the laboratories of Alterra. As a traditional management practice, slightly polluted sediments from ditches in the rural area of The Netherlands are spread over adjacent land. In experiments on the island of Goeree-Overflakkee, it has been shown that the practice of spreading of thin layers also led to fast, slow and very slow degradation of the PAH's present
Future Changes in Water Supply and Demand for Las Vegas Valley: A System Dynamic Approach based on CMIP3 and CMIP5 Climate Projections

The study investigated the impact on water supply and demand as an effect of climate change and population growth in the Las Vegas Valley (LVV) as a part of the Thriving Earth Exchange Program. The analyses evaluated future supply and demand scenarios utilizing a system dynamics model based on the climate and hydrological projections from the Coupled Model Intercomparison Project phases 3 and 5 (CMIP3 and CMIP5, respectively) using the simulation period expanding from 1989 to 2049. The main source of water supply in LVV is the water storage in Lake Mead, which is directly related to Lake Mead elevation. In order to assess the future water demand, the elevation of Lake Mead was evaluated under several water availability scenarios. Fifty-nine out of the 97 (27 out of the 48) projections from CMIP5 (CMIP3) indicated that the future mean elevation of Lake Mead is likely to be lower than the historical mean. Demand forecasts showed that the Southern Nevada Water Authority’s conservation goal for 2035 can be significantly met under prevalent conservation practices. Findings from this study can be useful for water managers and resource planners to predict future water budget and to make effective decisions in advance to attain sustainable practices and conservation goals.
Gender Diversity in Spanish Banks: Trickle-Down and Productivity Effects

The study of the gender composition of top management and its impact for organizations has received increasing attention during recent decades. Despite this, findings have been conflicting and few consistent conclusions have been reached. This paper uses panel data methods to study how the presence of women at the board of governance impacts gender diversity at different organizational levels in the Spanish banking sector. In addition, we explore the influence of female representation on employee productivity. Our findings show that the presence of one or two women at the board of directors benefits the advancement of women to managerial positions. We also identify a positive significant relationship between gender diversity at different organizational strata and productivity. The main conclusion of this research is that female representation at the board has relevant implications in terms of women being promoted and organizational outcomes. This contribution is relevant for both scholars and practitioners, as it explores the business case for gender diversity going beyond the upper echelons of organizations.
Pertanggung Jawaban BPJS Kesehatan terhadap Pelayanan Asuransi Kesehatan Masyarakat

Peran pemerintah terhadap warga negaranya dalam menjalankan pemerintahan salah satunya adalah dengan memberikan pelayanan publik. Salah satu pelayanan publik tersebut adalah pelayanan kesehatan. Sebagai bentuk tanggung jawab pemerintah dalam mewujudkan kesejahteraan masyarakat yaitu dengan program jaminan kesehatan melalui BPJS Kesehatan. BPJS Kesehatan adalah bagian dari JKN (Jaminan Kesehatan Nasional) yang merupakan kebijakan pemerintah di bidang kesehatan dan bertujuan mewujudkan terselenggaranya pemberian jaminan kesehatan yang layak bagi seluruh masyarakat Indonesia khususnya bagi setiap peserta dan/atau anggota keluarganya. JKN merupakan bagian dari Sistem jaminan Sosial Nasional (SJSN) yang diselenggarakan melalui mekanisme asuransi yang sifatnya wajib berdasarkan Undang-Undang Nomor 40 tahun 2004 tentang SJSN. Diterbitkannya 3 (Tiga) Peraturan Direktur Jaminan Pelayanan Kesehatan mengenai pengurangan pelayanan kesehatan telah menuai pro dan kontra, karena dinilai akan mencederai hak-hak dari peserta JKN. Perhimpunan Dokter Indonesia Bersatu (PDIB) keberatan dengan keluarnya 3 (tiga) peraturan Direktur Jaminan Pelayanan Kesehatan karena akan berdampak pada kinerja dokter. Peraturan yang dikeluarkan oleh Direktur Jaminan Pelayanan Kesehatan berimplikasi mencederai peraturan Undang-Undang Sistem Jaminan Sosial Nasional, maka dari itu penelitian ini bertujuan untuk meneliti apakah Direktur Jaminan Pelayanan Kesehatan berwenang mengeluarkan ketiga peraturan tersebut dan apakah peraturan tersebut bertentangan dengan Undang Undang Sistem Jaminan Sosial Nasional.
Ultra-wideband circular polarization-maintaining reflection realized by an anisotropic metasurface

In this paper, an ultra-wideband circular polarization (CP) maintaining reflector is proposed based on an anisotropic metasurface, which is an orthogonal anisotropic structure with a pair of symmetrical axes u and v perpendicular to each other. The simulated results show that the reflector can realized CP-maintaining reflection under both right-handed and left-handed CP (RHCP and LHCP) incidences in the frequency band between 8.79 and 27.09 GHz, the fractional bandwidth is up to 102%. In addition, the physical mechanism of the CP-maintaining reflection has been analyzed in detail, and one useful formula has been derived, which can be used to calculate the cross- and co-polarization reflection coefficients under CP incidence according to the phase differences between the two reflection coefficients under u and v-polarized incidences. Finally, one effective experimental validation was carried out.
Flexible high dielectric thin films based on cellulose nanofibrils and acid oxidized multi-walled carbon nanotubes

Flexible high dielectric materials are of prime importance for advanced portable, foldable and wearable devices. A series of flexible high dielectric thin films based on cellulose nanofibrils (CNF) and acid oxidized multi-walled carbon nanotubes (o-MWCNT) was prepared in aqueous solution. Though no organic solvent was involved during the preparation, the SEM images showed that o-MWCNTs have good distribution within the CNF matrix. The dielectric constant of CNF/o-MWCNT (6.2 wt%) composite films was greatly increased from 25.24 for pure CNF to 73.88, while the loss tangent slightly decreased from 0.70 to 0.68, and the AC conductivity decreased from 3.15 × 10−7 S cm−1 for CNF to 1.77 × 10−7 S cm−1 (at 1 kHz). The abnormal decrease of loss tangent and AC conductivity were attributed to the introduction of oxide-containing groups on the surface of MWCNTs. The nanocomposite films showed excellent flexibility such that they could be bent a thousand times without visible damage. The presence of MWCNTs also helped to improve the thermal stability of the composite films. The excellent dielectric and mechanical properties of the CNF/o-MWCNT composite film demonstrate its great potential to be utilized in the field of energy storage.
Understanding The Roles Of Transcriptional Regulators For The Development Of Natural And Novel Inhibitors Of Listeria Monocytogenes.

The foodborne pathogenic bacterium, Listeria monocytogenes, causes listeriosis, a rare, but serious, invasive disease affecting both humans and animals. The ability of L. monocytogenes to survive transmission through food systems and to cause disease is attributed to both its environmental stress survival capabilities and its virulence gene repertoire. Stress response and virulence functions in L. monocytogenes have been ascribed to the pleiotropic transcriptional regulators σ and PrfA, however, little is known about the involvement of other transcriptional regulators in pathogenicity. To assess contributions of various regulatory proteins to virulence and virulenceassociated phenotypes, L. monocytogenes laboratory parent strain 10403S and a collection of otherwise isogenic strains each bearing in-frame deletions in genes encoding alternative sigma factors σ, σ, or σ or repressors CtsR or HrcA were tested in: (i) invasion assays with Caco-2 intestinal epithelial cells; (ii) intracellular growth assays in J774 mouse macrophage-like cells; and (iii) intragastric infections in the guinea pig model. We found that σ was essential for optimal invasion efficiency in intestinal epithelial cells and only PrfA was obligatory for wildtype cytosolic growth and spread. In addition to PrfA and σ, we found that CtsR also contributes to virulence during intragastric infection in the guinea pig. In summary, while no clear virulence-associated phenotypes were attributed to L. monocytogenes σ, σ, σ, or HrcA under the conditions tested, it is possible that regulatory networks exist among these regulators to enable at least partial functional compensation in the absence of a given factor.
On the radial acceleration of disc galaxies

The physical processes defining the dynamics of disc galaxies are still poorly understood. Hundreds of articles have appeared in the literature over the last decades without arriving at an understanding within a consistent gravitational theory. Dark matter (DM) scenarios or a modification of Newtonian dynamics (MOND) are employed to model the non-Keplerian rotation curves in most of the studies, but the nature of DM and its interaction with baryonic matter remains an open question and MOND formulates a mathematical concept without a physical process. We have continued our attempts to use the impact theory of gravitation for a description of the peculiar acceleration and velocity curves and have considered five more galaxies. Using published data of the galaxies NGC 3198, NGC 2403, NGC 1090, UGC 3205, and NGC 1705, it has been possible to find good fits without DM for the observed disc velocities and, as example, also for the extraplanar matter of NGC 3198.
The effect of temperature and concentration of Aspergillus fumigatus on chlorpyrifos removal

Chlorpyrifos is a toxic organophosphate type of insecticide with a molecular formula of C9H11Cl3NO3PS. Bioremediation is an environmental friendly method used to combat this insecticide through microbial enzymatic activity. This study therefore aims to eliminate chlorpyrifos with temperature variations and concentrations of Aspergillus fumigatus. Aspergillus fumigatus fungi acts as bioremedian to degrade the activity of chlorpyrifos using temperature ranges of 25-35oC at a 0.5-1.5% concentration rate. However, using the Gas-Chromatography Mass Spectrometer (GC-MS) method, optimum chlorpyrifos degradation was achieved at 25oC with a 1.5% concentration for 5 days in potato dextrose broth (PDB) liquid media. This shows that A. fumigatus has the ability to remove chlorpyrifos with an efficiency of 95.92% from its initial concentration of 80 ppm.
The effect of altered blood oxygen content on respiratory gas exchange dynamics

Two theories describe the limiting factors existing during exercise, determined by factors effecting the entire respiratory gas exchange sequence from 0 2 extraction to its use at the mitochondria. The first implicates 0 2 transport (a central limitation), while the second focuses on oxygen utilisation at the periphery. This study aimed to identify which factors may limit gas exchange response dynamics during submaximal exercise. This was accomplished by altering blood oxygen content, either through manipulating haematocrit (Hct), or through changes in Fx0 2. Six physically active male subjects (age = 24.8 ± 2.8 (SD) yrs) undertook aerobic power (Vo2peak) tests in each of three stages of altered haematocrit: (i) control (Hct = 40.0 ± 2.0%, V02peak = 5.58 ± 0.6 Linin'1); (ii) anaemia (withdrawal of -1350 ml of whole blood: Hct = 35.0 ± 2.0% (p<0.05), V02peak = 4.68 ± 0.46 l.min'1 (p<0.05)); and (iii) polycythaemia (autologous reinfusion: Hct = 44.0 ± 2.0% (p<0.05), V02peak = 5.06 ± 0.74 l.min'1). Three submaximal tests, involving a single step­ change in workrate from rest to -45% of the condition-specific V02peak, were completed in each state of altered Hct, with subjects breathing hyperoxic (FjO2=0.3), normoxic (FxO2=0.2) or hypoxic gases (FjO2=0.1). Results show that following a step-change in workrate, there were no changes in oxygen consumption (V02), carbon dioxide elimination (VC02), minute ventilation (VE) or cardiac frequency (fc) dynamics following blood withdrawal or subsequent reinfusion, suggesting a compensatory mechanism in fc, may have nullified any change in oxygen delivery, or else oxygen utilization by the mitochondria may be rate limiting. Under hypoxic conditions, there were no differences in the times to reach 20%80% of the V02 post-step plateau, although the time to reach 100% was longer in the anaemic state (p<0.05). Similar responses were observed for fc and VE dynamics. The dynamics of VC02 for a step-increase in workrate, again showed that under hypoxic conditions, the increase tended to be faster than under normoxic or hyperoxic conditions. Following a step-decrease in workrate, hypoxia slowed all responses (p<0.05), while hyperoxia did not accelerate the responses relative to the normoxic responses (p>0.05). Cardiorespiratory response dynamics appear unaffected by minor changes in the Hct and blood 0 2 content. This may imply that, the speed with which exercising tissues adjust to a step-function is influenced by peripheral factors, rather than by central factors, with 0 2 delivery exceeding 0 2 utilisation requirements. However, with a larger reduction in blood 0 2 content, a slowing of the response dynamics may be ascribed to a predominantly central limitation. Thus, it appears that within certain 0 2 delivery ranges, V02 dynamics are resilient to minor changes in 0 2 delivery.
Challenging “Getting Better” Social Media Narratives With Intersectional Transgender Lived Experiences

A dominant media narrative of “getting better” over time is often projected onto LGBTQ people’s personal life experiences. In this research study, I examine this narrative’s role in transgender people’s emotional well-being throughout transition. A “getting better” narrative was pervasive in my qualitative analysis of 240 Tumblr transition blogs and 20 interviews with bloggers, signaling that it impacted people’s self-concept both as presented on social media and when talking about their experiences. This narrative causes undue emotional harm given contrast between one’s post-transition reality, which may involve distress (despite greater congruence between one’s body and identity), and a dominant cultural expectation of happiness. I argue that an intersectional approach to understanding trans people’s emotional well-being—by considering multiple salient identity facets and life transitions—makes trans lives more livable by complicating the cultural imperative to feel better, and to present a unilaterally positive self-image online, post-transition. Even though trans people on average feel better after gender transition, everyday realities are often in contrast to the dominant narrative’s positioning of gender transition as a process with a single, simple goal of feeling better. Challenging the “getting better” narrative gives trans people the freedom to live and exist in their post-transition identities, whether or not they feel “better. ”
Universal Screening for Writing Risk in Spanish-Speaking First Graders

Abstract The main objective of this study was to analyze what writing measures, or combination of measures, would be most appropriate for early detection of students at risk of presenting future difficulties in learning to write in Spanish, in terms of tasks, scoring indices (dependent, independent and accurate-precision) and duration. A sample of 231 first graders was scored through IPAE (Indicadores de Progreso de Aprendizaje en Escritura [Indicators of Basic Early Writing Skills]), a curriculum-based assessment, in fall, winter, and spring. The students were classified as at-risk or not at risk based on a standard instrument (EGWA, Early Grade Writing Assessment) administered to them in spring. The predictive usefulness of the IPAE measures and their diagnostic accuracy were analyzed using the ROC Curve and binary logistic regression. The results suggest that dictated arbitrary spelling, dictated rule-based spelling, and dictated nonsense words could be an effective single measure to be used as a universal screening in the first stage of a two-stage gated screening procedure. A combined measure that includes a combination of these tasks and allographs in 1 min and dictated sentences can be efficient in a second stage.
Analysis and Design of Rateless Two-way Relay Networks Based on a Multiply-and-Forward Scheme

In this paper, we propose a rateless, three-stage, two-way multiply-and-forward (MF) relaying system over the Rayleigh flat fading channel, where two source nodes communicate with each other through a relay node and all nodes work on half-duplex and time-division mode. We thoroughly analyze the signals during all three stages in the proposed MF system and derive the closed-form symbol error rate (SER) expressions for an uncoded MF-two-way relay network (MF-TWRN). Furthermore, we provide the equivalent point-to-point fading channel model, which is employed to carry out the asymptotic performance analysis. We finally put forth an optimization model for the MF-TWRN with fountain codes. Simulation results show that our optimized degree distribution can provide outstanding performance for the MF-TWRN compared to those in the literature.
Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia

Abstract. Julisaniah NI, Suharjono, Mastuti R, Arumingtyas EL. 2020. Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia. Biodiversitas 21: 903-909. Peanut stripe virus (PStV) is a single-stranded positive-sense RNA virus capable of infecting peanut plants. An isolate of PStV (PStV-Bm) was collected from a peanut field in the Bima District, West Nusa Tenggara Province, Indonesia and the coat protein (CP) gene of this virus (CP-PStV) was extracted from the viral RNA and analyzed using reverse transcription-polymerase chain reaction methods. The CP-PStV gene of PStV-Bm was aligned with several PStV genes deposited in the Genbank (http://www.ncbi.nml.nih.gov). Based on the nucleotide sequence of the CP gene, PStV-Bm was grouped into a similar cluster with other PStVs that originated from Indonesia with a similar index, ranging from 96.8% to 98.9%. Genetic similarity (about 96.1%) was also observed between PStV-Bm and PStV from the USA. This genetic similarity indicated that viruses from adjacent regions have high genetic relationships. Some amino acid differences were observed in PStV-Bm that may be typical of this strain.
IDENTIFICATION OF INHIBITORS OF DENGUE VIRUS (DENV1, DENV2 AND DENV3) NS2B/ NS3 SERINE PROTEASE: A MOLICULAR DOCKING AND SIMULATION APPROACH

Dengue is one of the fatal diseases, which are becoming a global health burden from few decades. Dengue fever, dengue hemorrhagic fever and dengue shock syndrome, caused by dengue virus (DENV), which completes its life cycle in mosquito i.e. Aedes aegyti, and human (DENV), and infect about various individuals every year. The objective of this study is to find a potent inhibitor of DENV (DENV1, DENV2 and DENV3). In the present study, NS2b/NS3 serine protease complex in targeted for the screening of the suitable inhibitors for DENV (DENV1, DENV2 and DENV 3). Therefore, the NS2b/NS3 serine protease complex structures were retrieved from the RCSB Protein Databank. The unliganded protein structures were docked, and best three selected and analyzed. A molecular dynamic simulation is also performed to investigate the conformational and positional changes of ligand that provide insights into the binding stability. It was observed that three of screened compounds have the maximum potential against the protein. The analysis was performed on the basis of scoring and binding ability and one of them indicated minimum energy score with high number of interactions with active site residues and the simulation study revealed that this selected ligand could efficiently bind to the NS2b/NS3 protease. These findings conclude that this selected ligand could be a promising inhibitor of all three serotypes of DENV as drug targets. Keywords: Dengue virus, Aedes aegyti, Flaviviridae, Serine protease, Docking.
Systematic value of losses of some notogastral setae in adult Sphaerozetinae (Acari: Oribatidae: Ceratozetidae) in the light of ontogenetic studies.

The morphology of adult and juvenile stages of 11 species of the ceratozetid subfamily Sphaerozetinae was investigated in order to explain systematic relationships of genera and species. The genera Ghilarovizetes, Melanozetes and Fuscozetes form well-defined Melanozetes group which differs morphologically from Edwardzetes and Sphaerozetes. The losses of some notogastral setae in adult of Melanozetes group were established and a hipothetical development of this group of mites is proposed.
Cytological and histological study of morphogenesis in Rhododendron L.

The genetic stability of regenerants in Rhododendron catawbiense Michx. 'Grandiflorum' and in vitro regeneration process from leaf expiants were determined. Chromosome diploid number (2n=2x=26) in actively growing shoot apices and a high degree of cultivar genetic stability were found. The effect of different concentrations of thidiazuron (TDZ) in the presence of indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D) or 2-isopentenyladenine (2iP) on embryogenic/organogenic induction was compared. Histological evidence demonstrated embryogenic capacity of mucilaginous callus at a concentration of 11.4-22.7 jiM of thidiazuron. Somatic embryo initiation was exclusively induced with TDZ, while IBA, 2,4-D or 2iP alone did not have any effect on formation of the embryogenic structures.