Miracle-dz/UniFAR-FTUs huggingface dataset:
Utilization of Whole-Cell MALDI-TOF Mass Spectrometry to Differentiate Burkholderia pseudomallei Wild-Type and Constructed Mutants

Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has been widely adopted as a useful technology in the identification and typing of microorganisms. This study employed the whole-cell MALDI-TOF MS to identify and differentiate wild-type and mutants containing constructed single gene mutations of Burkholderia pseudomallei, a pathogenic bacterium causing melioidosis disease in both humans and animals. Candidate biomarkers for the B. pseudomallei mutants, including rpoS, ppk, and bpsI isolates, were determined. Taxon-specific and clinical isolate-specific biomarkers of B. pseudomallei were consistently found and conserved across all average mass spectra. Cluster analysis of MALDI spectra of all isolates exhibited separate distribution. A total of twelve potential mass peaks discriminating between wild-type and mutant isolates were identified using ClinProTools analysis. Two peaks (m/z 2721 and 2748 Da) were specific for the rpoS isolate, three (m/z 3150, 3378, and 7994 Da) for ppk, and seven (m/z 3420, 3520, 3587, 3688, 4623, 4708, and 5450 Da) for bpsI. Our findings demonstrated that the rapid, accurate, and reproducible mass profiling technology could have new implications in laboratory-based rapid differentiation of extensive libraries of genetically altered bacteria.
SOME OF THE FORCED CONVECTION HEAT TRANSFER CHARACTERISTICS OF CONDENSED MILKS.

Abstract The heating and cooling characteristics of two condensed milk products were investigated, using a tubular heat exchanger equipped with wall and stream thermocouples. The mass flow rates were regulated to effect a Reynolds number range of 5,000 to 200,000. Nusselt-type equations which described the heating and cooling characteristics of each of the products investigated follow, respectively: condensed milk (7.9% fat and 25.9% total solids), Nu = 0.0803 Re 0.663 Pr 0.4 and Nu = 0.0466 Re 0.659 Pr 0.4 ; condensed milk (10.7% fat and 35.0% total solids), Nu = 0.0267 Re 0.729 Pr 0.4 , and Nu = 0.0243 Re 0.732 Pr 0.4 . A general equation which described the heating and cooling characteristics of the product was Nu = 0.0845 ( Re a ) 0.67 Pr 0.4 where a was a factor for correcting the water equation of Al-Arabi to reflect the heat transfer characteristics of the dairy products. The respective a values for the products during heating and cooling were condensed milk (7.9% fat and 25.9% total solids) = 0.937 and 0.886; condensed milk (10.7% fat and 35.0% total solids) = 0.896 and 0.867. A discussion is included in the report concerning the possibility of a need to re-evaluate some of the earlier work on heat transfer characteristics of dairy products.
Running head: DEATH BY SUICIDE 1 Adlerian Perspective on the Interpersonal Theory of Suicide in Relation to Adolescents and Young Adults

Suicide is a leading cause of death for adolescents and young adults. This paper reviews empirical literature pertaining to suicide ideation, suicide attempts, and suicide. Current research identifies risk factors which act as triggers for suicidal behavior. Assessing the potential moderating role of protective factors such as resilience and coping skills will be discussed. Gatekeeper training and screening assessments, two of the most promising programs in the pursuit of suicide prevention, will be summarized. This review of literature describes Dialectical Behavior Therapy and Cognitive Behavior Therapy, protocols proven effective in the treatment of adolescent suicidal behaviors. An examination of the Interpersonal Theory of Suicide and Adlerian Theory highlights their impact on suicide assessment, prevention, and treatment.
Design and implementation of a high power three-level T-type inverter for a photovoltaic system

Nowadays, the grid-connected photovoltaic systems are an important part of the renewable energy sources, and their performance is getting more and more important. Many reported researches focused on the traditional NPC topology. However, few researches were about the T-type NPC inverter. In this paper, a high power three-phase T-type neutral point clamped grid connected photovoltaic inverter prototype is designed and implemented. The design and implementation procedure of the inverter is discussed in detail. Experimental results showed that the output power of the proposed inverter could reach up to 100kW with unit power factor, while the total harmonic distortion of the grid current was less than 3%. And the total efficiency of the system is measured as 95.6%. The experimental results demonstrated the T-type NPC inverter can operate with a good performance.
A Novel Real-Time Searching Method for Power System Splitting Boundary

It is possible to prevent collapse of the whole post-fault power system, which is in unstable condition, by determining the splitting boundary so as to calm down the system oscillation. A fast method of searching for the splitting boundary of power system controlled islanding is presented in this paper, the aim of which is to minimize the load-generation imbalance in each island. The method contains three phases, namely, defining the domain of each generator according to power flow tracing algorithm, determining an initial splitting boundary based on the grouping information of generators, and refining the initial one to get the final splitting boundary. A real-time searching program for power system splitting boundary was developed based on the method. Simulations on the IEEE 118-bus power system and a practical power system show that the proposed method is effective and fast.
Alcohol consumption and metabolic syndrome among Shanghai adults: a randomized multistage stratified cluster sampling investigation.

AIM To examine the relations of alcohol consumption to the prevalence of metabolic syndrome in Shanghai adults. METHODS We performed a cross-sectional analysis of data from the randomized multistage stratified cluster sampling of Shanghai adults, who were evaluated for alcohol consumption and each component of metabolic syndrome, using the adapted U.S. National Cholesterol Education Program criteria. Current alcohol consumption was defined as more than once of alcohol drinking per month. RESULTS The study population consisted of 3953 participants (1524 men) with a mean age of 54.3 +/- 12.1 years. Among them, 448 subjects (11.3%) were current alcohol drinkers, including 405 males and 43 females. After adjustment for age and sex, the prevalence of current alcohol drinking and metabolic syndrome in the general population of Shanghai was 13.0% and 15.3%, respectively. Compared with nondrinkers, the prevalence of hypertriglyceridemia and hypertension was higher while the prevalence of abdominal obesity, low serum high-density-lipoprotein cholesterol (HDL-C) and diabetes mellitus was lower in subjects who consumed alcohol twice or more per month, with a trend toward reducing the prevalence of metabolic syndrome. Among the current alcohol drinkers, systolic blood pressure, HDL-C, fasting plasma glucose, and prevalence of hypertriglyceridemia tended to increase with increased alcohol consumption. However, low-density-lipoprotein cholesterol concentration, prevalence of abdominal obesity, low serum HDL-C and metabolic syndrome showed the tendency to decrease. Moreover, these statistically significant differences were independent of gender and age. CONCLUSION Current alcohol consumption is associated with a lower prevalence of metabolic syndrome irrespective of alcohol intake (g/d), and has a favorable influence on HDL-C, waist circumference, and possible diabetes mellitus. However, alcohol intake increases the likelihood of hypertension, hypertriglyceridemia and hyperglycemia. The clinical significance of these findings needs further investigation.
Non-contact 10% efficient 36mW power delivery using on-chip inductor in 0.18-μm CMOS

This paper presents design and implementation of an inductive coupling power delivery system between stacked chips in 0.18-mum CMOS process. Two conventional high-power rectifier structures are compared, and a new topology is proposed. With a pair of fully optimized 700 mum times 700 mum on-chip inductors, the test chip achieves 10% peak efficiency and 36 mW power transmission. Compared with previous published chip-to-chip wireless power transmission systems, the received power is 13 times larger.
Contributions of hybrid architectures to depth imaging: a CPU, APU and GPU comparative study. (Apports des architectures hybrides à l'imagerie profondeur : étude comparative entre CPU, APU et GPU)

In an exploration context, Oil and Gas (O&G) companies rely on HPC to accelerate depth imaging algorithms. Solutions based on CPU clusters and hardware accelerators are widely embraced by the industry. The Graphics Processing Units (GPUs), with a huge compute power and a high memory bandwidth, had attracted significant interest.However, deploying heavy imaging workflows, the Reverse Time Migration (RTM) being the most famous, on such hardware had suffered from few limitations. Namely, the lack of memory capacity, frequent CPU-GPU communications that may be bottlenecked by the PCI transfer rate, and high power consumptions. Recently, AMD has launched theAccelerated Processing Unit (APU): a processor that merges a CPU and a GPU on the same die, with promising features notably a unified CPU-GPU memory. Throughout this thesis, we explore how efficiently may the APU technology be applicable in an O&G context, and study if it can overcome the limitations that characterize the CPU and GPU based solutions. The APU is evaluated with the help of memory, applicative and power efficiency OpenCL benchmarks. The feasibility of the hybrid utilization of the APUs is surveyed. The efficiency of a directive based approach is also investigated. By means of a thorough review of a selection of seismic applications (modeling and RTM) on the node level and on the large scale level, a comparative study between the CPU, the APU and the GPU is conducted. We show the relevance of overlapping I/O and MPI communications with computations for the APU and GPUclusters, that APUs deliver performances that range between those of CPUs and those of GPUs, and that the APU can be as power efficient as the GPU.
Imipenem Resistance of Enterobacter aerogenes Mediated by Outer Membrane Permeability

ABSTRACT Multidrug-resistant Enterobacter aerogenes strains are increasingly isolated in Europe and especially in France. Treatment leads to imipenem resistance, because of a lack of porin. We studied the evolution of resistance in 29 strains isolated from four patients during their clinical course. These strains belonged to the prevalent epidemiological type observed in France in previous studies (C. Bosi, et al., J. Clin. Microbiol. 37:2165–2169, 1999; A. Davin-Regli et al., J. Clin. Microbiol. 34:1474–1480, 1996). They also harbored a TEM-24 extended-spectrum β-lactamase-coding gene. Thirteen strains were susceptible to gentamicin and resistant to imipenem and cefepime. All of the patients showed E. aerogenes strains with this resistance after an imipenem treatment. One patient showed resistance to imipenem after a treatment with cefpirome. Twelve of these 13 strains showed a lack of porin. Cessation of treatment with imipenem for three patients was followed by reversion of susceptibility to this antibiotic and the reappearance of porins, except in one case. For one patient, we observed three times in the same day the coexistence of resistant strains lacking porin and susceptible strains possessing porin. The emergence of multidrug-resistant E. aerogenes strains is very disquieting. In our study, infection by E. aerogenesincreased the severity of the patients' illnesses, causing a 100% fatality rate.
Egg Yolk Phospholipids Enriched with 1O-Octadecyl-2-Oleoyl-sn-Glycero-3-Phospho-( N-Palmitoyl ) Ethanolamine Inhibit Development of Experimentally Induced Tumours ( hen egg phospholipids / phospholipid derivative NAEPE / inhibition of tumour cells / inhibition of liver / lung / kidney tumours / chic

Dietary phospholipids (PLs) and their derivatives have proved active in suppression of various health problems and conditions including cancer. In this work we compared the effect of dietary phospholipids from hen egg yolk enriched with N-acyl ether-phosphatidyl ethanolamine (NAEPE) termed bioactive phospholipids (BAP+ preparation) with PLs lacking NAEPE (BAP– preparation) on the growth of transformed cells in vitro and on the promotion and progression of experimental tumours in vivo. For the in vivo experiments we used the chicken model in which liver, lung, and kidney tumours arose via natural selection from single cells initiated by experimentally introduced somatic mutations caused by insertional mutagenesis. Mutagenized animals were fed BAP+ or BAP– diet in various regimens. We observed that BAP+ at low concentrations killed cells of various tumour cell lines in culture but did not compromise viability of non-transformed cells. Oral administration of the BAP+ preparation efficiently reduced progression of all tumour types. However, it did not significantly reduce the number of already initiated tumours and their growth when BAP+ was discontinued. Our data suggest that NAEPE comReceived February 12, 2014. Accepted May 18, 2014. This work was supported by research concept AV0Z50520514 from the Academy of Sciences of the Czech Republic, v. v. i. Corresponding author: Michal Dvořák, Institute of Molecular Genetics AS CR, v. v. i. , Vídeňská 1083, 142 20 Prague 4, Czech Republic. Phone: (+420) 296 443 390; Fax: (+420) 296 443 586; e-mail: mdvorak@img.cas. cz Abbreviations: BAP – bioactive phospholipids (phospholipid fraction from hen egg yolk), CCl4 – carbon tetrachloride, CEF – chicken embryo fibroblasts, FA – fatty acid, ICC – intrahepatal cholagiocarcinoma, MAV-2 – myeloblastosis-associated virus-2, NAEPE – N-acyl ether-phosphatidyl ethanolamine, NRK – normal rat kidney, PE – phosphatidyl ethanolamine, PL – phospholipid. bined with hen egg PLs significantly interferes with tumour progression, possibly through the inhibition of tumour cell viability.
Squeezed spin states.

The basic concept of squeezed spin states is established and the principles for their generation are discussed. Two proposed mechanisms, referred to as one-axis twisting and two-axis countertwisting, are shown to reduce the standard quantum noise S/2 of the coherent S-spin state down to 1/2(S/3${)}^{1/3}$ and 1/2, respectively. Implementations of spin squeezing in interferometers are also discussed.
Toward a Causal Explanation of Plant Invasiveness: Seedling Growth and Life‐History Strategies of 29 Pine (Pinus) Species

We studied 29 pine (Pinus) species to test the hypothesis that invasive species in disturbed habitats have distinct attributes. Seedling relative growth rate (RGR) and measures of invasiveness were positively associated across species as well as within phylogenetically independent contrasts. High RGR, small seed masses, and short generation times characterize pine species that are successful invaders in disturbed habitats. Discriminant analysis and logistic regression revealed that RGR was the most significant factor among these life‐history traits separating invasive and noninvasive species. We also explored the causes of differences in RGR among invasive and noninvasive species. While net assimilation rate, leaf mass ratio, and specific leaf area (SLA) were all found to be contributing positively to RGR, SLA was found to be the main component responsible for differences in RGR between invasive and noninvasive pines. We investigated differences in SLA further by studying leaf anatomy, leaf density, and leaf thickness. We also evaluated relative leaf production rate as an important aspect of SLA. We proposed a hypothetical causal network of all relevant variables.
Pelvis feature extraction and classification of Cardiff body match rig base measurements for input into a knowledge-based system

The purpose of this paper is to determine whether it is possible to use an automated measurement tool to clinically classify clients who are wheelchair users with severe musculoskeletal deformities, replacing the current process which relies upon clinical engineers with advanced knowledge and skills. Clients’ body shapes were captured using the Cardiff Body Match (CBM) Rig developed by the Rehabilitation Engineering Unit (REU) at Rookwood Hospital in Cardiff. A bespoke feature extraction algorithm was developed that estimates the position of external landmarks on clients’ pelvises so that useful measurements can be obtained. The outputs of the feature extraction algorithms were compared to CBM measurements where the positions of the client’s pelvis landmarks were known. The results show that using the extracted features facilitated classification. Qualitative analysis showed that the estimated positions of the landmark points were close enough to their actual positions to be useful to clinicians undertaking clinical assessments.
LytM-Domain Factors Are Required for Daughter Cell Separation and Rapid Ampicillin-Induced Lysis in Escherichia coli

ABSTRACT Bacterial cytokinesis is coupled to the localized synthesis of new peptidoglycan (PG) at the division site. This newly generated septal PG is initially shared by the daughter cells. In Escherichia coli and other gram-negative bacteria, it is split shortly after it is made to promote daughter cell separation and allow outer membrane constriction to closely follow that of the inner membrane. We have discovered that the LytM (lysostaphin)-domain containing factors of E. coli (EnvC, NlpD, YgeR, and YebA) are absolutely required for septal PG splitting and daughter cell separation. Mutants lacking all LytM factors form long cell chains with septa containing a layer of unsplit PG. Consistent with these factors playing a direct role in septal PG splitting, both EnvC-mCherry and NlpD-mCherry fusions were found to be specifically recruited to the division site. We also uncovered a role for the LytM-domain factors in the process of β-lactam-induced cell lysis. Compared to wild-type cells, mutants lacking LytM-domain factors were delayed in the onset of cell lysis after treatment with ampicillin. Moreover, rather than lysing from midcell lesions like wild-type cells, LytM− cells appeared to lyse through a gradual loss of cell shape and integrity. Overall, the phenotypes of mutants lacking LytM-domain factors bear a striking resemblance to those of mutants defective for the N-acetylmuramyl-l-alanine amidases: AmiA, AmiB, and AmiC. E. coli thus appears to rely on two distinct sets of putative PG hydrolases to promote proper cell division.
Sustained Attention Function Evaluation During Cooking Based on Egocentric Vision

The attention function has been classified into (i) sustained attention, (ii) selective attention, (iii) controlled attention, (iv) distributed attention, and (v) capacity for attention. Ordinarily, in order to evaluate the function of attention, the digital cancellation test (D-CAT) or trail making test (TMT) are employed. However, these evaluations are in the form of paper tests, and cannot effectively evaluate small changes in activities daily of living in real time. Accordingly, we focus on human behavior during the act of cooking, in which multitasking is common and many types of ordinary movements are performed. We previously proposed a method of evaluating distributive attention during cooking and showed its effectiveness. In this study, a method for evaluating sustained attention is proposed. Specifically, we acquire an egocentric video by an egocentric vision and estimate line-of-sight based on the visual attention model that is integrated from the three kind of models (category-specific attention, top-down attention, and bottom-up attention). After that, we define line-to-sight information from high saliency area on the visual attention model.
Earthquake Awareness Programs as a Key for Earthquake Preparedness and Risk Reduction : Lessons from Nepal

Nepal is a highly seismic country having a long history of destructive earthquakes. Huge loss of life and property had occurred in series of earthquakes in the past and it is believed that next big earthquake is around the corner. Studies have estimated a heavy loss of life and property due to the possible future earthquake in the country. The impact of destructive earthquakes can only be reduced through coordinated efforts among all stakeholders and individuals for effective awareness and preparedness. Realizing this fact, National Society for Earthquake Technology-Nepal (NSET) has been conducting earthquake preparedness awareness programs in massive scale focusing different level of stakeholders and individuals in the country. This paper presents the experiences on such activities carried out by NSET in Nepal, which will work as the guiding efforts for the similar countries in the world.
Genome Sequence of Lactococcus garvieae 8831, Isolated from Rainbow Trout Lactococcosis Outbreaks in Spain

ABSTRACT Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp with a G+C content of 38%.
Psychological Correlates of Mental Stress-induced Ischemia in the Laboratory

Participants consisted of 184 patients (160 males, 24 females) with positive angiograms or prior myocardial infarctions who displayed at least 1 mm of ST segment depression on a standardized treadmill test. Mean scores on the Reward Dependence subscale of the Tridimensional Personality Questionnaire were higher in patients displaying ischemia during mental stress. Patients who reported higher levels of irritability/anger in response to the Speech stressor were also more likely to display ischemia. However, this result was primarily a result of the females in the sample whose ratings of interest and irritability were associated with ischemia during the Speech task. Psychometric measures previously found in prospective studies to predict acute cardiac events were unrelated to mental stress-induced ischemia in the laboratory.
A genetic variation of cathepsin D is a major risk factor for Alzheimer's disease

Cathepsin D (catD) is an intracellular acid protease possibly involved in Alzheimer's disease (AD)‐related neurodegeneration through cleavage of amyloid precursor protein into amyloidogenic components. We studied whether an exonic polymorphism of the catD gene (C → T [Ala → Val] transition at position 224), which possibly influences pro‐catD secretion and intracellular maturation of the enzyme, was associated with the risk for the development of AD in 127 demented patients and 184 controls. The catD*T allele was significantly overrepresented in demented patients (11.8%) compared with nondemented controls (4.9%). Carriers of the catD*T allele had a 3.1‐fold increased risk for developing AD than noncarriers. Carriers of the apolipoprotein E (ApoE) ε4 allele (ApoE*4) had a 3.9‐fold increased risk than non‐carriers. The adjusted odds ratio for subjects with the ApoE*4 and the catD*T allele was 19.0 compared with subjects with neither of these two alleles. Our data confirm the results of a recently performed pilot study in an independent sample and suggest that the catD genotype is strongly associated with the risk for AD. Ann Neurol 2000;47:399–403