Miracle-dz/UniFAR-FTUs huggingface dataset:
Consecutive Electrophoretic Separation of PCR Products Under a High-Ionic-Strength Solution on PMMA Chips with Enhanced Static Adsorptive Coat

Abstract Consecutive polymerase chain reaction (PCR) product electrophoretic separation was done using a high-ionic-strength solution on poly(methyl methacrylate) (PMMA) micofluidic devices. Microchannels were modified with an enhanced static adsorptive coating method using 3% hydroxyethyl cellulose. The relative standard deviations of migration time and fluorescence intensity for the amplified cytosine deaminase PCR product of 258 bp (without pretreatments in 16 consecutive and rapid runs) on the PMMA chip were 0.88% and 3.5%, with a separation efficiency of 6.0 × 105/m. PCR products were repeatedly separated on the modified chip without rinsing the channels with water and refilling the channels with the sieving matrix between runs.
Integrated oil separation and water purification by a double-layer TiO2-based mesh

We report a simple and inexpensive method for fabricating double-layer TiO2-based mesh. The upper layer is a TiO2 coated mesh film with micro- and nano-structures, while the lower is the same TiO2 mesh film but modified with ODP (octadecyl phosphonic acid) to impart its superhydrophobic and superoleophilic properties. Such a mesh can be successfully used for the separation of insoluble oil from water due to its special wettability, as well as the degradation of soluble pollutants in water under UV light because of the photocatalytic abilities of TiO2, making it a promising candidate for water purification.
The Visible Light Activity of the TiO2 and TiO2:V4+ Photocatalyst

TiO2 and vanadium-doped TiO2 nanoparticles were synthesized by using the hydrothermal method. The V doping contents are 0.0; 0.1; 0.3; 0.5; 0.7 and 0.9% molar.The vanadium-doped TiO2 nanoparticles have identical anatase phase with an average crystal size of 10–20nm. The oleic acid and ethanol solvents with different molar concentrations can make spherical nanograins, or stick form grains, which influence the photo activity of the materials. The absorption spectra of doped samples exhibited long-tailed absorption in the visible light region above 380nm.The visible light photocatalytic activity was evaluated by the degradation of phenol aqueous solutions; after 360 min. under the visible irradiation, the normalized concentration of phenol decreased to 9%.
Reproducible layer-by-layer exfoliation for free-standing ultrathin films of single-walled carbon nanotubes

We developed a facile fabrication method for highly transparent free-standing SWNT ultrathin films. The SWNT ultrathin films can be reproducibly exfoliated from the dense SWNT film on the filter by surface tension of deionized water, which were easily transferred onto PET substrates to make highly sensitive flexible chemiresistive sensors.
On the direct energy transfer to moving acceptors

This paper studies the decay law of the excitation of a donor molecule, due to its microscopic long range interactions with moving acceptor molecules. Particular attention is given to the case of acceptor molecules randomly dispersed in a host liquid. From a general approach we derive an exact ensemble averaged formula for the decay of the donor excitation as a function of the molecular motion. The expression obtained is valid for all types of microscopic molecular interactions and for arbitrary ratios of acceptors to inert molecules; from this formula we determine the energy decay for motions which are slow or rapid relatively to the microscopic energy transfer times. If the motion is frozen, we retrieve the decay law valid for acceptors imbedded randomly in a solid matrix [A. Blumen and J. Manz, J. Chem. Phys. (to be published)]. For slow molecular displacements, which obey the Langevin equations of Brownian motion, the diffusion dependent decay law is presented; in the case of low acceptor concentratio.
Effect of the Addition of Bentonite and Polyvinylpolypyrrolidone on the Colour and Long-term Stability of Red Wines

The effect of the fining agents bentonite and polyvinylpolypyrrolidone (PVPP) on wine phenolics and colour characteristics has been studied, and the characteristics compared with those of an untreated wine. The use of fining agents resulted in lower concentrations of hydroxycinnamic derivatives, procyanidins and flavan-3-ols but higher concentrations of monomeric anthocyanins. The addition of fining agents in the pre-fermentative phase may partially prevent the disappearance of anthocyanins through the elimination of solids to which they could be fixed. The evolution of the wine characteristics was followed during 12 months' storage and it was observed that clarified wines maintained the differences from the untreated wine during the storage time. At the end of the experiment, clarified wines, especially the wine clarified with PVPP, had a higher monomeric and ionised anthocyanin content.
Design and Validation of a 6-Volatility Tandem Differential Mobility Analyzer (VTDMA)

A volatility tandem differential mobility analyzer (VTDMA) was developed to allow fast field measurement of the volatile fraction of atmospheric aerosol particles in the particle size range 20–500 nm. In this VTDMA the volatile compounds are evaporated by heating the aerosol to a temperature between 25°C and 300°C. The heating unit is equipped with six symmetric columns kept at different temperatures that allow the heating temperature to be rapidly changed so that a higher temporal resolution can be achieved compared to a regular VTDMA. This work first focuses on the design and calibration of the heating units for the conditioning of a selected aerosol sample while minimizing sample losses due to thermophoresis and diffusion. The design was based on the modeling of the profiles of temperature and velocity and the behavior of a monodisperse aerosol in the heating units, using Computational Fluid Dynamics (CFD) Flow Modeling Software. This allowed for initial estimations of the heater dimensions and also calculation of the minimum length of heating tube needed to completely evaporate the aerosol particles at high temperature with sufficient residence time, as well as to cool the aerosol sample down to ambient temperature. Next, the aerosol heating rate and aerosol deposition losses within the flow tube were estimated, and re-condensation of volatilized compounds evaluated. Then the VTDMA was calibrated and tested in the laboratory to determine the transfer efficiency, and finally, atmospheric aerosols were analyzed, with the first results presented here. This work emphasizes the need for better standardization of thermo-desorbing units for atmospheric aerosol studies. *Now at: Laboratoire de Chimie et Environnement, Université de Provence.
Improved fits for the vibrational and rotational constants of many states of nitrogen and oxygen

All pertinent measurements of the vibrational intervals ΔG(v+ 1/2 ) and rotational constants Bv for 11 states of N2, four states of N+2, the ground state of O2, and four states of O+2 that could be found in published papers have been assembled and plotted against v. (These are the states important in modeling the fluorescence produced when air is bombarded by fast electrons.) These values of ΔG and Bv are compared with values calculated from the standard polynomials in powers of v+ 1/2 , using the coefficients tabulated by Huber and Herzberg (1979), as well as coefficients derived by later analysts, when available. In about 25 percent of the states considered, the coefficients of Huber and Herzberg are found to still yield good fits to the latest available spectroscopic data. In another 25 percent, good fits are obtained from more recently published coefficients. For the remaining 50 percent of the states, new improved coefficients have been derived by least‐squares fitting. The results are tabulated and .
Determination of bosutinib in mice plasma and tissue by UPLC-MS/MS and its application to the pharmacokinetic and tissue distribution study

A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine bosutinib in mice plasma and tissue using diazepam as the internal standard (IS). Sample preparation was accomplished through a protein precipitation procedure with acetonitrile. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with the mobile phase of acetonitrile and 0.1% formic acid in water with gradient elution at a flow rate of 0.40 mL min−1. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reaction monitoring (MRM) of the transitions at m/z 530.3 → 141.1 for bosutinib and m/z 285.2 → 193.1 for the IS. The linearity of this method was found to be within the concentration range of 5–3000 ng mL−1 with a lower limit of quantification of 5.0 ng mL−1. Only 3.0 min was needed for an analytical run. The method herein described was superior to previous methods and was successfully applied to the pharmacokinetic and tissue distribution study of bosutinib in mice after oral administration.
Carbon Monoxide Adsorption on Molybdenum Phosphides: Fourier Transform Infrared Spectroscopic and Density Functional Theory Studies

Molybdenum phosphide (MoP) and supported molybdenum phosphide (MoP/γ-Al2O3) have been prepared by the temperature-programmed reduction method. The surface sites of the MoP/γ-Al2O3 catalyst were characterized by carbon monoxide (CO) adsorption with in situ Fourier transform infrared (FT-IR) spectroscopy. A characteristic IR band at 2037 cm-1 was observed on the MoP/γ-Al2O3 that was reduced at 973 K. This band is attributed to linearly adsorbed CO on Mo atoms of the MoP surface and is similar to IR bands at 2040−2060 cm-1, which correspond to CO that has been adsorbed on some noble metals, such as platinum, palladium, and rhodium. Density functional calculations of the structure of molybdenum phosphides, as well as CO chemisorption on the MoP(001) surface, have also been studied on periodic surface models, using the generalized gradient approximation (GGA) for the exchange-correlation functional. The results show that the chemisorption of CO on MoP occurred mainly on top of molybdenum, because the bonding o.
Qualitative assessment of rhizodeposits in non-sterile soil by analytical pyrolysis.

Estimation of the amount of root exudates and simultaneous identification of their composition in non-sterile soil is a challenging objective in rhizosphere research. We coupled 3 methods: (1) labeling of corn in 14CO2 atmosphere to separate root-derived and soil-derived organic substances in the rhizosphere, (2) a previously developed leaching method to collect rhizodeposits, and (3) pyrolysis field ionization mass spectrometry (Py-FIMS) to investigate the molecular-chemical composition of rhizodeposits. Eluted rhizodeposits accounted for 2.8 % (Loam) and 0.97 % (nutrient solution in quartz sand) of recovered 14C and showed clear differences in composition between the growth substrates. The 14CO2 evolved mostly by root respiration accounted for 3.5–4.0 % without significant differences according to growth substrate or diurnal dynamics. Principal component analysis of the Py-FI mass spectra of leachates showed a clear diurnal dynamics of the amount and the composition of corn rhizodeposits collected during day-time and night-time. Differences originated mostly from signals assigned to carbohydrates, sterols, and peptides. This approach is recommended for forthcoming studies of rhizodeposition in different soil substrates, crops grown, and time-series of exudate sampling. Qualitative Untersuchung der Rhizodeposition im nicht sterilen Boden mit analytischer Pyrolyse Quantifizierung und gleichzeitige chemische Identifikation der Rhizodeposite im nicht sterilen Boden sind nach wie vor eine Herausforderung in der Rhizospharenforschung. Als neuen methodischen Ansatz kombinierten wir (1) die Markierung von Mais in einer 14CO2-Atmosphare zur Trennung wurzelburtiger und bodenburtiger organischer Substanzen in der Rhizosphare mit (2) einer neuen Perkolationsmethode zur separaten Erfassung der Rhizodeposite und (3) Pyrolyse-Feldionisations-Massenspektrometrie (Py-FIMS) zur Untersuchung der molekular-chemischen Zusammensetzung der Rhizodeposite. Zwischen 2.8 % (Toniger Schluff) und 1.0 % (Nahrlosung in Quarzsand) des wiedergefundenen 14C entfiel auf die eluierten Rhizodeposite. Das uberwiegend durch Wurzelatmung freigesetzte 14CO2 betrug 3.5–4.0 % des wiedergefundenen 14C, wobei kaum Unterschiede hinsichtlich Substrat bzw. Tageszeit deutlich wurden. Auswertung der Py-FI-Massenspektren der Eluate mit Hauptkomponentenanalyse zeigte deutliche Unterschiede in der Zusammensetzung der Eluate aus den beiden unterschiedlichen Substraten sowie aus der Deposition am Tage und in der Nacht. Diese Unterschiede wurden vor allem durch die Intensitaten von Markersignalen fur Kohlenhydrate, Sterole und Peptide hervorgerufen. In weitergehenden Untersuchungen soll dieser experimentelle Ansatz zur Erforschung der Rhizodeposition unter verschiedenen Bodenbedingungen, mit verschiedenen Pflanzenarten und in Zeitreihen der Beprobung genutzt werden.
Fourier Transform Rheology of Branched Polyethylene: Experiments and Models for Assessing the Macromolecular Architecture

Fourier transform rheology (FTR) consists of analyzing the frequency spectrum of the nonlinear torque response obtained during large-amplitude oscillatory shear. In the present work, the analysis has been carried out in the whole complex space and was especially focused on the study of the third complex harmonic I*3/I1 (= I*(3ω1)/I1, where I1 is the spectrum intensity at the fundamental frequency ω1), the intensity I3/I1 (= |I*3/I1|), and the phase shift Φ3 of I*3/I1. The accuracy of FTR to characterize the architecture of linear, sparsely, and densely branched polyethylene regarding the number of branches and their length has been shown. A modified Wagner integral model and a differential multimode pompom model were used to simulate FTR experiments. Nonlinear parameters of each model were determined by fitting the curve of the third complex harmonic I*3/I1 versus the strain amplitude γ0 with the experimental data. Good results have been obtained with the modified Wagner model. Whereas, the pompom model a.
Controlled Switching of Multicomponent Heterocyclizations of 5‐Amino‐N‐arylpyrazole‐4‐carboxamides, 1,3‐Cyclohexanediones, and Aldehydes

Multicomponent heterocyclization of 5-amino-N-arylpyrazole-4-carboxamides, 1,3-cyclohexanediones, and aromatic aldehydes was studied, and reaction conditions allowing selective switching between two directions were found. When the reaction was carried out under thermal heating or ultrasonication at room temperature, formation of linear pyrazoloquinoline-3-carboxamides was observed. Isomeric angular
Metal Removal Studies by Composite Membrane of Polysulfone and Functionalized Single-Walled Carbon Nanotubes

Composite nanofiltration membranes were prepared by incorporating single-walled carbon nanotubes (SWNTs) into polysulfone polymer via the phase inversion method. Addition of SWCNTs resulted in the reduction of the pore size due to nanosize (0.7-1.3 nm) diameter of SWCNTs used. The increase in carbon nanotubes makes the surface of the membranes smoother, although the solvent and nonsolvent exchange process becomes slower due to increase in viscosity of the blend solution. The addition of SWCNTs improved the rejection of metal ions, the oxidized functionalized C/PS composite membranes gave 96.8% removal for Cr(VI), 87.6% for As(III), and 94.2% removal for Pb(II) which was just 30.3%, 28.5%, and 28.3%, respectively, with unblended virgin polysulfone membrane.
Characterization and thermoelectric properties of p-type 25%Bi2Te3–75%Sb2Te3 prepared via mechanical alloying and plasma activated sintering

In the present work, starting from elemental bismuth, antimony and tellurium powders, p-type 25%Bi2Te3–75%Sb2Te3 thermoelectric materials with high density were prepared by mechanical alloying (MA) and plasma activated sintering (PAS). The single phase 25%Bi2Te3–75%Sb2Te3 alloys were obtained after MA for 12 h. The effect of sintering temperatures on microstructure and thermoelectric properties of the as-PASed samples was researched. Highly compact samples with relative density over 99% could be obtained when sintering temperature was over 653 K. A preferentially orientated microstructure with the (1 1 0) plane parallel to and the basal planes (0 0 l) perpendicular to the pressing direction was formed, and the orientation factors of the (0 0 l) planes changed from 0.11 to 0.12 at different sintering temperatures. The maximum power factor and figures of merit (Z) at room temperature were 3.10 × 10−3 W m−1 K−2 and 2.85 × 10−3 K−1, respectively. The Vickers microhardness reached 112.7 Hv, which was twice that of the single crystal samples prepared by zone-melting.
Time-dependent calculations of molten pool formation and thermal plasma with metal vapour in gas tungsten arc welding

A gas tungsten arc (GTA) was modelled taking into account the contamination of the plasma by metal vapour from the molten anode. The whole region of GTA atmosphere including the tungsten cathode, the arc plasma and the anode was treated using a unified numerical model. A viscosity approximation was used to express the diffusion coefficient in terms of viscosity of the shielding gas and metal vapour. The transient two-dimensional distributions of temperature, velocity of plasma flow and iron vapour concentration were predicted, together with the molten pool as a function of time for a 150 A arc current at atmospheric pressure, both for helium and argon gases. It was shown that the thermal plasma in the GTA was influenced by iron vapour from the molten pool surface and that the concentration of iron vapour in the plasma was dependent on the temperature of the molten pool. GTA on high sulfur stainless steel was calculated to discuss the differences between a low sulfur and a high sulfur stainless steel anode. Helium was selected as the shielding gas because a helium GTA produces more metal vapour than an argon GTA. In the GTA on a high sulfur stainless steel anode, iron vapour and current path were constricted. Radiative emission density in the GTA on high sulfur stainless steel was also concentrated in the centre area of the arc plasma together with the iron vapour although the temperature distributions were almost the same as that in the case of a low sulfur stainless steel anode.
Introduction to Floquet theory: the calculation of spinning sideband intensities in magic-angle spinning NMR

In magic-angle spinning, the Hamiltonian ofthe system changes periodi - cally with time, so the spectrum shows sidebands at multiples ofthe rotor f requency. This () changing Hamiltonian and hence, the changing Liouvillian complicates the simulation of 1 the line shape considerably. A simple case is that ofa spin- with an anisotropic chemical 2 shift, spinning at the magic angle. Perhaps the most famous solutions to this problem of calculating spinning sideband intensities are those ofHerzf eld and Berger and Maricq and Waugh, but there are many alternatives. Floquet theory is a very powerful approach, which we describe here. We illustrate the theory by explicit calculation ofthe sideband patterns. () Floquet theory works by expanding the periodic due to sample spinning Hamiltonian into a Fourier series. The time-independent Fourier components become the blocks in a much larger, time-independent, effective Hamiltonian. The same can be done with the Liouvillian () superoperator. The effective Hamiltonian or Liouvillian can then be treated using familiar methods used for time-independent problems. The considerable increase in size of the problem is normally anathema, and it offers no particular benefit in the specific case treated here. The power ofthe method is its generality. Regardless ofthe complexity ofthe time dependence ofthe Hamiltonian, the Floquet approach is the same. Theref ore, learning how to calculate sideband intensities in this way gives us the tools to solve much more difficult problems. 2001 John Wiley & Sons, Inc. Concepts Magn Reson 13: 159 170, 2001
Graft Copolymerization of Polyacrylamide onto Tamarind Mucilage

In the present communication, grafting of acrylamide onto a water‐soluble food grade polysaccharide, Tamarind mucilage, initiated by ceric ion in aqueous medium has been studied under N2 atmosphere. A ceric ion initiated solution polymerization technique was found to be satisfactory for the formation of copolymer. The effect of monomer concentration, initiator concentration, reaction time, and temperature in terms of grafting efficiency (%GE) and percent of grafting (PG) have been investigated. The graft copolymers were characterized by FTIR, scanning electron microscope (SEM), differential scanning calorimetry (DSC), thermo gravimetric analyzer (TGA), and biodegradation studies.
Development of an electrochemical immunosensor for Phakopsora pachyrhizi detection in the early diagnosis of soybean rust

A ferrugem da soja e uma doenca que ocorre nas folhas da soja e e considerada muito agressiva, pois reduz a qualidade do grao final. A identificacao precoce do fungo na plantacao previne varias perdas para o agricultor bem como para as culturas vizinhas. Neste trabalho, um imunossensor foi desenvolvido sem a utilizacao de marcadores usando medidas de impedância para detectar a ferrugem asiatica nos extratos das folhas contaminadas nos estagios iniciais da doenca, antes do aparecimento dos sintomas. O anticorpo anti-micelio do fungo Phakopsora pachyrhizi (agente causador da doenca) foi imobilizado em substrato de ouro via formacao de monocamadas auto-organizadas de tiois e ligacoes covalentes com a biomolecula. O imunossensor apresentou um limite de deteccao de 385 ng mL-1. A otimizacao das condicoes experimentais e o bloqueio da superficie para minimizar ligacoes nao-especificas foram realizados. Este estudo proporcionou uma nova perspectiva da utilizacao do metodo para o diagnostico precoce da doenca.
Combinatorial Molecular Imprinting: An Approach to Synthetic Polymer Receptors

A novel method for synthesizing and evaluating artificial receptors is demonstrated, combining a molecular imprinting concept and a combinatorial chemistry strategy. Combinatorial libraries of molecularly imprinted polymers (MIPs) were prepared and screened for high affinity and selectivity to the original template by a newly developed semiautomatic system. The preparation of MIPs was automatically performed using programmed liquid-handling equipment with a new in situ molecular imprinting protocol whereby MIP is prepared on the bottom surface of each glass vial, followed by an automated discrete binding test with the programmed liquid handler. MIPs as artificial receptors for triazine herbicides, ametryn and atrazine, were prepared by the combinatorial molecular imprinting using a diverse amount of two functional monomers, methacrylic acid (MAA) and 2-(trifluoromethyl)acrylic acid (TFMAA). Examining the MIP libraries, it appears that, depending upon the functional monomer used, the imprinting efficiency .