Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of Sulfanilamide in Blood and Urine

Recently a method was described for the estimation of sulfanilamide (para-aminobenzenesulfonamide) in blood and urine. 1 In the rabbit and human subject this drug is partly excreted in the form of an acetylated derivative. 2 In developing a method for determining this conjugated derivative in blood, we have been able to improve the original procedure. The present modification possesses the advantages of being more sensitive, of giving a more stable color, and of requiring fewer manipulations. Sulfanilamide can be determined very accurately by this method, and in addition one can obtain a fairly good estimate of the conjugated form present in blood. The present method consists of preparing a blood filtrate with toluenesulfonic acid, utilizing the acidity of the precipitant to perform diazotization and coupling with the amine. In determining the conjugated sulfanilamide in blood, the acidity of the blood filtrate is sufficient for hydrolysis on heating. One volume of oxalated blood is measured into a flask, diluted and laked with 7 volumes of 0.05% saponin solution. ∗ After laking is complete (1 or 2 minutes) 2 volumes of para-toluenesulfonic acid solution (20 gm. dissolved in water and diluted to 100 cc.) are added with shaking. After 5 minutes' standing, the mixture is filtered, and 10 cc. of the clear filtrate (a smaller amount can be used with proportionate reduction of the nitrite and dimethyl-a-naphthylamine reagents) is measured into a small flask and 1 cc. of 0.1% freshly prepared† sodium nitrite solution is added. After 3 minutes' standing, 5 cc. of a solution of dimethyl-α-naphthylamine (1 cc. in 250 cc. of 95% ethyl alcohol) is added from a burette.
A role for caveolin-3 in the pathogenesis of the mdx mouse

Duchenne muscular dystrophy (DMD) is a muscle-wasting disease caused by the loss of sarcolemmal protein dystrophin. In DMD and the mouse model of the disease mdx, there is an increase in an associated protein, caveolin-3. In this study, mdx mice with deficiencies in caveolin-3 were generated to allow a distinction to be made between the pathology caused by the loss of dystrophin and that caused by an excess of caveolin-3. It was found that in late gestation embryos, there were perturbations in skeletal muscle stem cell populations and depletion of respiratory muscles in mdx and mdx/cav3\(^{+/-}\), both of which were more severe in mdx/cav3\(^{+/-}\) embryos. In post natal skeletal muscles, there was a trend in that the level of regeneration, believed to be indicative of previous degeneration, was consistently greater in mdx than mdx/cav3\(^{+/-}\). Taken together it would appear whereas increased caveolin-3 may compensate for the lack of dystrophin in embryonic mdx muscle; post natally, it may contribute to the muscle regeneration observed in mdx. The data presented in this thesis should help towards clarifying the contribution of caveolin-3 in the pathogenesis of DMD and in doing so expand on the understanding of the molecular aetiology of the disease.
Effects of Exercise Intervention for Children with Acute Lymphoblastic Leukemia: A Systematic Review

Background: The purpose of this systematic review is to examine the research literature on the effects of therapeutic exercise on strength, function, and endurance, when implemented during and immediately following chemotherapy, in children with acute lymphoblastic leukemia (ALL). This review of the literature, which includes children with ALL and the effects of therapeutic exercise across the levels of the International Classification of Function (ICF), has not been previously undertaken. Five‐year survivability of ALL has significantly increased and a review to address the long‐term effects of ALL and its treatment is merited. Procedure: We searched 3 electronic databases and reviewed reference lists in relevant articles and online sources published between 1999 and 2012. Thirteen intervention studies were appraised that included 345 participants. Results: Across all studies, therapeutic exercise was a safe intervention for patients with ALL, with each study noting positive outcomes. However, the validity of the studies overall was low, with a preponderance of studies lacking sufficient design rigor, a variety of exercise parameters, poor tracking of adherence to intervention, and incomplete statistical analyses. Conclusions: Additional randomized controlled trials with greater statistical rigor, which examine practical exercise parameters, address participant compliance, and relate outcomes across levels of the International Classification of Function are needed to better determine the potential benefits of therapeutic exercise for children with acute lymphoblastic leukemia.
Toward understanding the role of protein context in the polyglutamine disease, SCA3

The polyglutamine diseases are a clinically heterogeneous group of inherited neurodegenerative disorders caused by expansion of polyglutamine-encoding (CAG)n trinucleotide repeats within the disease genes. It is increasingly clear that the amino acid sequences flanking the polyglutamine expansion in each disease protein, i.e. the specific protein context, contribute to selective neuronal toxicity by influencing the behavior of the disease protein within selectively vulnerable neuronal populations. In the studies described here, I explore the role that protein context plays in the polyglutamine disease, Spinocerebellar ataxia type 3 (SCA3). Toward this end, I utilize biochemical, cell-based, and animal models to gain a broader understanding of the SCA3 disease protein, ataxin-3, and generate tools for further exploration of the molecular properties of ataxin-3 that modulate its toxicity during disease. In Chapter 1, I provide an overview of the recognized polyglutamine diseases, emphasizing the elements of protein context that are distinct among the polyglutamine disease proteins and may contribute to the neuropathological and clinical heterogeneity within this family of diseases. Alternative splicing of the polyglutamine disease gene products adds an additional level of complexity to the tissue-specific protein context of expanded polyglutamine, yet this phenomenon has been underinvestigated. In Chapter 2, I examine the significance of ataxin-3 splice variation. Several minor 5’ variants and both known 3’ splice variants of ataxin-3, a deubiquitinating enzyme, are expressed at the mRNA level in brain. At the protein level, however, the C-terminal splice isoform with 3 ubiquitin interacting motifs (3UIM ataxin-3) is the predominant isoform in brain, independent of age or (CAG)n expansion. Although both C-terminal ataxin-3 splice isoforms display similar in vitro deubiquitinating activity, 2UIM ataxin-3 is more prone to aggregate and is more rapidly degraded by the proteasome. These observations demonstrate how alternative splicing of
Calcium-related fungal genes implicated in arbuscular mycorrhiza

Fluctuations in intracellular (Ca2+) calcium levels generate signaling events and regulate different cellular processes. Whilst the implication of Ca2+ in plant cell responses during arbuscular mycorrhiza (AM) interactions is well documented, nothing is known about the regulation or role of this secondary meesenger in the fungal symbiont. The molecular basis of fungal calcium homeostasis in the AM symbiosis was analyzed by investigating the expression of Ca2+-related fungal genes. In a first study, G. mosseae genes putatively encoding a MAP3k-like protein kinase (Gm2) and a P-type ATPase (Gm152) were investigated. Both Ca2+-related genes were up-regulated by A. sinicum root exudates, suggesting a role in early interactions prior to symbiosis establishment. The full-length cDNA sequence of Gm152 obtained from germinating spores of G. mosseae confirmed its identity. The role of Ca2+ in fungal processes leading to establishment of an AM symbiosis was investigated in more detail in G. intraradices-M. truncatula interactions. Enhanced expression of genes encoding six membrane transport proteins and one nuclear protein kinase, selected from the G. intraradices transcriptome database, was related to colonization of wild-type M. truncatula (line J5) roots and not observed with the mycorrhiza-resistant mutant dmi3/Mtsym13. Laser microdissection mapping of transcripts indicated that the Ca2+-related G. intraradices genes were differentially up-regulated in arbuscules and/or in intercellular hyphae. The tempo-spatial variations in fungal gene expression suggest different roles in the development or functioning of the AM symbiosis. Full-length cDNA of three G. intraradices genes putatively encoding a PMR-like endoplasmic reticulum P-type ATPase, a VCX1-like vacuolar Ca2+ ion transporter and a nuclear CCaMK were obtained for functional analyses in yeast mutants to gain insight into their role in the mycorrhizal symbiosis. Possible mechanisms are discussed in which Ca2+-related proteins of G. intraradices may play a role in the mobilization and perception of the intracellular messenger by the AM fungus during symbiotic interactions with host roots
ASSESSMENT OF LONELINESS, QUALITY OF SLEEP AND AFFECTING FACTORS IN ELDERS

Introduction: Assessment of loneliness, quality of sleep and affecting factors in elders. Materials and Method: A total of 428 elders were included in the research. The research data were collected using the loneliness scale (UCLA-LS) and Pittsburgh sleep quality index (PSQI). One-way analysis of variance, t test and Pearson analysis of correlation were used to evaluate the data. Results: The average loneliness score was 41.87±8.43. The average loneliness score of those living in the nursing home was higher than that of those living in the home environment. The relationship between the average loneliness score and age, educational background, childbearing, and the number of visitors were significant (p<0.05). The average sleep quality score was 6.21±3.33. Educational background, income status, childbearing, having a chronic disease, number of visitors, and duration of residence in the nursing home were found to affect the quality of sleep (p<0.05). A slightly positive relationship (r=0.298; p=0.001) was observed between the average loneliness score and the quality of sleep score. Conclusion: Loneliness and sleep problems are common among elders. In order to improve their quality of life and health status, health care providers should recognize and take necessary actions to alleviate these problems.
Reliability of Aesthetic component of IOTN in the assessment of subjective orthodontic treatment need

Objective: The purpose of this study was to estimate whether dental concern expressed by the grade of the Aesthetic Component [AC] chosen by subjects is reliable and whether it may be predictive for potential cooperation. Study Design: The study was carried out among 100 (50 boys and 50 girls) subjects aged 18 to 20 years, who visited Darshan Dental College & Hospital, Udaipur. Subjects were selected randomly who fall in said age group during the period of one month and was based on a clinical examination and questionnaire. The questionnaire contained items relating to the influence of the dentition on the general appearance, the subjective assessment of dental appearance, demand for orthodontic treatment, and any functional disorders. Clinical examination was carried out at Darshan Dental College & Hospital, Udaipur by the orthodontist. Results: The results indicate that using professional rating the Aesthetic component scale does not seem to be more precise or reliable than self-evaluation. The outcome shows that the Aesthetic component of the IOTN moderately reflects the subjective perception of dental aesthetics and demand for orthodontic treatment. Potential cooperation was there in 44% of the subjects as they readily agree to undergo orthodontic treatment if suggested. Conclusion: There was a significant agreement in the Aesthetic Component between the professional rating and Student’s assessments, as 76 percent were consistent when considering the category criterion.
Metabolic syndrome in patients with autoimmune inflammatory rheumatisms

Background: Systemic inflammation has been associated with obesity, with elevated levels of triglycerides and reduced levels of HDL-cholesterol. Aim: To determine the frequency of metabolic syndrome (MetS) in patients suffering of rheumatoid arthritis (RA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) and to compare the evolution of MetS parameters during DMARD (Disease-Modifying Anti-rheumatic Drugs) therapy versus combination of DMARDs and biologics. Methods: The study was conducted on 277 RA patients, 115 AS patients and 103 PsA patients. Patients with inflammatory rheumatisms and MetS were randomized into two groups per disease (RA, AS and PsA). Group 1 underwent DMARD therapy. Group 2 followed a combination of DMARDs and biologics. Patients were assessed for the inflammatory status (C-reactive protein) and MetS (triglycerides, HDL cholesterol and blood pressure) initially, after 1 y and after 2 y. Results: 88 RA patients (31.7%), 16 AS patients (14%) and 35 PsA patients (34%) met the criteria for MetS. RA, AS and PsA patients with MetS who followed a combination of DMARDs and biologic therapy had significant improvement of triglycerides and HDL-cholesterol compared to patients who followed only DMARDs both at 1-y and 2-y assessments. There were no differences of the blood pressure in the two groups. Conclusion: The frequency of MetS was higher in RA and AS patients than in PsA patients. Biologic therapy could influence the evolution of MetS in patients suffering of autoimmune inflammatory rheumatisms.
Development of Recording and Reporting of Nosocomial Infection Surveillance System in Surabaya Premier Hospital

Nosocomial infection is infection that occurs in patients who are hospitalized. One of infection control programs is the surveillance activity. Refer to the Instructions Practical Hospital nosocomial infections Surveillance by the Health Ministry (2011), computer usage in surveillance activities will increase the efficiency of data collection and analysis. The aim of this study was to develop recording and reporting nosocomial infection surveillance system. This study type was action research with System Development Life Cycle (SDLC) method where in the stages of SDLC were planning, analysis, design, implementation and usage. The instruments used in this study were by creating DFD, ERD, data dictionary then continued with the development of applications using PHP and MySQL. Data collection was committed through interviews and observations. The results of this research was web-based applications tested using the method of Technology Acceptance Model (TAM). Keywords: Nosocomial infection surveillance, Recording and reporting, System development
Race/Ethnicity, Dietary Acid Load and Risk of ESRD among U.S. Adults with CKD

Background—Dietary acid load (DAL) confers risk for CKD and CKD progression. We sought to determine the relation of DAL to racial/ethnic differences in risk of ESRD among persons with CKD. Methods—Among 1123 non-Hispanic black (NHB) and white (NHW) National Health and Nutrition Examination Survey III participants with estimated glomerular filtration rate 15-59 ml/min/1.73 m2, DAL was estimated using the Remer and Manz net acid excretion (NAEes) formula and 24-hour dietary recall. ESRD events were ascertained via linkage with Medicare. A competing risk model (accounting for death) was used to estimate the hazard ratio (HR) for treated ESRD, comparing NHBs with NHWs, adjusting for demographic, clinical and nutritional factors Corresponding Author: Deidra C. Crews, MD, ScM, Division of Nephrology, Department of Medicine, Johns Hopkins University School of Medicine, 301 Mason F. Lord Drive, Suite 2500, Baltimore, Maryland 21224, U.S.A. Disclosures: The authors report no conflicts of interest relative to this manuscript. HHS Public Access Author manuscript Am J Nephrol. Author manuscript; available in PMC 2019 March 09. Published in final edited form as: Am J Nephrol. 2018 ; 47(3): 174–181. doi:10.1159/000487715. A uhor M anscript
Exploring the Needs of Adolescents With Sickle Cell Disease to Inform a Digital Self-Management and Transitional Care Program: Qualitative Study

Background Accessible self-management interventions are critical for adolescents with sickle cell disease to better cope with their disease, improve health outcomes and health-related quality of life, and promote successful transition to adult health care services. However, very few comprehensive self-management and transitional care programs have been developed and tested in this population. Internet and mobile phone technologies can improve accessibility and acceptability of interventions to promote disease self-management in adolescents with sickle cell disease. Objective The aim of this study was to qualitatively explore the following from the perspectives of adolescents, parents, and their health care providers: (1) the impact of sickle cell disease on adolescents to identify challenges to their self-management and transitional care and (2) determine the essential components of a digital self-management and transitional care program as the first phase to inform its development. Methods A qualitative descriptive design utilizing audio-recorded, semistructured interviews was used. Adolescents (n=19, aged 12-19 years) and parents (n=2) participated in individual interviews, and health care providers (n=17) participated in focus group discussions and were recruited from an urban tertiary care pediatric hospital. Audio-recorded data were transcribed verbatim and organized into categories inductively, reflecting emerging themes using simple content analysis. Results Data were categorized into 4 major themes: (1) impact of sickle cell disease, (2) experiences and challenges of self-management, (3) recommendations for self-management and transitional care, and (4) perceptions about a digital self-management program. Themes included subcategories and the perspectives of adolescents, parents, and health care providers. Adolescents discussed more issues related to self-management, whereas health care providers and parents discussed issues related to transition to adult health services. Conclusions Adolescents, parents, and health care providers described the continued challenges youth with sickle cell disease face in terms of psychosocial impacts and stigmatization. Participants perceived a benefit to alleviating some of these challenges through a digital self-management tool. They recommended that an effective digital self-management program should provide appropriate sickle cell disease–related education; guidance on developing self-advocacy and communication skills; empower adolescents with information for planning for their future; provide options for social support; and be designed to be engaging for both adolescents and parents to use. A digital platform to deliver these elements is an accessible and acceptable way to address the self-management and transitional care needs of adolescents.
Psychological Interventions for Recurrence Prevention in Adolescent Depression: A Systematic Review

Objective: To determine the effectiveness of psychological interventions in depressive adolescents to prevent recurrent depressive episodes.Material and Methods: English databases, including Embase, Medline, Global Health, and PsychINFO, were searched for studies that examined the effectiveness of psychological interventions in preventing recurrent depression. Eight articles were found in the electronic databases and 4 studies were found from a manual search. Methodological quality was assessed using the Cochrane Collaboration’s tool.Results: One quasi-randomized control trial and 11 randomized control trials met the inclusion criteria. A systematic review of the 12 studies was conducted. The studies involved 1,641 participants that represented different effects of recurrence prevention interventions at 6, 9, 12, 24, and 33 months following treatment completion. Cognitive behavioral therapy (CBT) group intervention showed effectiveness in recurrence prevention in depressive adolescents in 6-33 months. Supportive psychotherapy and family therapy also prevented recurrent depression at 24-month follow-up, while psychodynamic/psychoanalytic psychotherapy and interpersonal psychotherapy could prevent recurrence in depressive adolescents for 12 months following completion of the intervention.Conclusion: Among the psychological interventions studied in the included articles, CBT group intervention showed the greatest long-term effect since group CBT could prevent recurrent depression in adolescents up to 33 months.
Coping and Adaptation Patterns among Chinese, Indian, and Malay Families Caring for a Mentally Ill Relative

Families caring for chronically mentally ill relatives often experience considerable stress and hardship. To adapt, family members must learn new coping strategies and make sufficient use of informal and formal support systems in the community. The authors examined cultural variations in the coping and adaptation process among three main ethnic groups in Singapore (Chinese, Malay, and Indian). Interviews conducted with primary caregivers in 30 families showed cross-cultural differences in the adverse effects on the family system, the use of coping strategies, and the utilization of support networks. A culturally sensitive, indigenous model of community mental health services designed to overcome family resistance is presented.
Dynamics of Physical Activity Status in Patients with Grade І-ІІІ Obesity in Response to a Physical Rehabilitation Program

Lazareva, O. Dynamics of Physical Activity Status in Patients with Grade І-ІІІ Obesity in Response to a Physical Rehabilitation Program / O. Lazareva, M. Aravitska, O. Andrieieva, Y. Galan, L. Dotsyuk // Journal of Physical Education and Sport. – 2017. – V. 17. – P. 1960 – 1965.
Leader's integrity and employee silence in healthcare organizations.

PURPOSE Drawing on the social exchange theory, the purpose of this paper is to examine the relationship between a leader's behavioral integrity and employee acquiescent silence. Specifically, the authors take a relational approach by introducing employee's relational identification as the mediator. The moderating role of employee political skill, in the relationship between behavioral integrity and employee acquiescent silence, is also considered. DESIGN/METHODOLOGY/APPROACH The data of this study encompass 913 front-line nurses from 13 university hospitals in Turkey. Hierarchical multiple regression analysis was conducted to test the proposed model. FINDINGS The results of this study support the negative effect of behavioral integrity on employee's acquiescent silence, as well as the mediating effect of employee's relational identification. Moreover, when the level of employee political skill is low, the relationship between behavioral integrity and acquiescent silence is strong, whereas the effect is weak when the level of political skill is high. PRACTICAL IMPLICATIONS The findings of this study suggest that healthcare administrators' words and deeds should be consistent while interacting with their subordinates, as it leads to positive interpersonal relationship, which, in turn, lowers employee silence. Moreover, healthcare administrators should pay more attention to the buffering role of employee political skill for those subordinates with low relational identification and higher workplace silence. ORIGINALITY/VALUE This study contributes to the literature on workplace silence by revealing the relational mechanism between behavioral integrity and employee silence. This paper also offers a practical assistance to employees in the healthcare industry and their administrators interested in building trust and high-quality manager-employee relationship, as well as lowering workplace silence.