Miracle-dz/UniFAR-FTUs huggingface dataset:
High-Throughput Screens Development and Validation of Reagents and Assays for EZH 2 Peptide and Nucleosome

Histone methyltransferases (HMT) catalyze the methylation of histone tail lysines, resulting in changes in gene transcription. Misregulation of these enzymes has been associated with various forms of cancer, making this target class a potential new area for the development of novel chemotherapeutics. EZH2 is the catalytic component of the polycomb group repressive complex (PRC2), which selectively methylates histone H3 lysine 27 (H3K27). EZH2 is overexpressed in prostate, breast, bladder, brain, and other tumor types and is recognized as a molecular marker for cancer progression and aggressiveness. Several new reagents and assays were developed to aid in the identification of EZH2 inhibitors, and these were used to execute two high-throughput screening campaigns. Activity assays using either an H3K27 peptide or nucleosomes as substrates for methylation are described. The strategy to screen EZH2 with either a surrogate peptide or a natural substrate led to the identification of the same tractable series. Compounds from this series are reversible, are [H]-S-adenosyl-Lmethionine competitive, and display biochemical inhibition of H3K27 methylation.
SB-ATR FTIR Spectroscopic Monitoring of Free Fatty Acids in Commercially Available Nigella sativa (Kalonji) Oil

Free fatty acids (FFA) in Nigella sativa (N. sativa) commercial and seed oil were determined using single-bounce attenuated total reflectance (SB-ATR) Fourier transform infrared (FTIR) spectroscopy. Gravimetrical mixing was done by adding 0.1–40% oleic acids in neutralized N. sativa oil containing 0.1% FFA. FTIR spectroscopy technique and partial least square (PLS) calibration were used to detect the absorption region of carbonyl (C=O) which is in the range of 1690–1727 cm−1. The results of PLS calibration model and root mean square error of calibration (RMSEC) are 0.999 and 0.449, respectively. Comparing the FFA obtained in N. sativa oil by using FTIR with the FFA obtained using AOCS titrimetric method shows a positive correlation and confirms that the described method is a useful procedure.
Improve Understanding of Fat Grafting ' s Impact on Skin Rejuvenation Adipose-Derived Stem Cell to Epithelial Stem Cell Transdifferentiation : A Mechanism to Potentially

Background: Recent evidence suggests that lipofilling improves overlying skin composition and appearance. Adipose-derived stem cells (ADSC) have been implicated. Objective: The authors identify ADSC transdifferentiation into epithelial stem cells through coexpression of GFP+ (green fluorescent protein positive) ADSC with the epithelial stem cell marker p63 in an in vivo fat grafting model. Methods: Six male, GFP+ mice served as adipose tissue donors. Twelve nude mice served as recipients. Recipients were subdivided into 2 arms (6 mice/each arm) and received either whole-fat specimen (group 1) or isolated and purified ADSC + peptide hydrogel carrier (group 2) engrafted into a 1-cm2 left parascapular subdermal plane. The right parascapular subdermal plane served as control. Skin flaps were harvested at 8 weeks and subjected to (1) confocal fluorescent microscopy and (2) reverse transcriptase polymerase chain reaction (RT-PCR) for p63 mRNA expression levels. Results: Gross examination of skin flaps demonstrated subjectively increased dermal vessel presence surrounding whole-fat and ADSC specimens. The GFP+ cells were seen within overlying dermal architecture after engraftment and were found to coexpress p63. Significantly increased levels of p63 expression were found in the ADSC + hydrogel skin flaps. Conclusions: We offer suggestive evidence that GFP+ ADSC are found within the dermis 8 weeks after engraftment and coexpress the epithelial stem cell marker p63, indicating that ADSC may transdifferentiate into epithelial stem cells after fat grafting. These findings complement current understanding of how fat grafts may rejuvenate overlying skin.
Comparison of In Vitro Dry Matter Degradation of Four Maize Silages Using the Menke Gas Production Method

Four maize silages from the UK and France were evaluated using the gas production method to estimate the rate and extent of dry matter degradation in vitro. There were significant differences in gas production after 17 h incubation. The silages showed similar times to produce 50, 95 and 100% of total gas production, whereas the lag time for Ema was significantly (P < 0.001) lower than that for Volgate. The potential gas production value (a + b) for Nancis was significantly (P < 0.05) lower than that for Volgate. There was a significant correlation (r = 0.996, R 2 = 90.71, P < 0.001) between gas production and total VFA production. There were considerable differences in the fermentability of carbohydrates between different maize silages. Nancis and Ema had more readily fermentable carbohydrate, which yielded high propionate production and a shorter lag time. 6196 and Volgate had more slowly fermentable carbohydrate, which did become available to microorganisms but after a longer lag time. Menke Gaz Uretim Teknii ile Dort Farkl› M›s›r Silaj›n›n in vitro Kuru Madde Parcalanmas›n›n Karfl›laflt›r›lmas› Ozet: ‹ngiltere ve Fransa'da yetifltirilen m›s›rdan yap›lan 4 silaj›n in vitro gaz uretim methodu kullanarak kuru maddenin parcalanma h›z› ve miktar› tahmin edilmifltir. 17 saatlik inkubasyondan sonra uretilen gaz miktar› m›s›r ceflitlerine gore onemli farkl›l›klar gostermifltir (P < 0.001). Toplam gaz›n% 50, 95 ve 100'unu uretmek icin gecen sure butun silajlar icin ayn› olmas›na ramen, gaz uretiminin bafllamas› icin gecen sure (lag time) silaj Ema icin, silaj Volgateden onemli derecede k›sa olmufltur (P < 0.001). Ayr›ca silaj Nancis icin potensiyel gaz uretimi, silaj Volgateden daha az bulunmufltur (P < 0.05). Uretilen gaz ile uretilen ucucu yaasitleri aras›nda onemli bir korelasyon bulunmufltur (r = 0.996, P < 0.001). Silaj karbonhidratlar›n›n fermente olma flekilleri aras›nda onemli farkl›l›klar olup silaj Nancis ve Ema' da bulunan karbonhidratlar mikroorganizmalar taraf›ndan daha kolay bir flekilde kullan›lm›flt›r. Sonuc itibariyle yuksek miktarda propiyonik asit uretimine neden olmufltur.
Investigating reading comprehension through EEG

Experimental studies point that different factors can influence reading comprehension, such as the topic, text type, reading task, and others. The advances in technologies for the past decades have provided researchers with several possibilities to investigate what goes on in one’s brain since their eyes meet the page until comprehension is achieved. Since the mid-80’s, numerous studies have been conducted with the use of the electroencephalogram (EEG) to investigate the process of reading, through the analysis of different components – n400, n100 or n1, P2, among others. These components reveal, for example, how the brain integrates the meaning of a specific word in the semantic context of a given sentence. based on previous studies, which demonstrate that different types of words affect cognitive load, this paper aims at investigating how the brain processes function and content words inserted in expository and narrative texts with suitable / unsuitable conclusions. results showed that the type of text and word influence the cognitive load in different scalp areas (midline, right and left hemispheres). The n1s were more pronounced to the content words inserted in narrative texts and to the function words inserted in the expository type of texts, corroborating former studies.
Developing Cyber Forensics for SCADA Industrial Control Systems

A large number of industries including: critical national infrastructure (electricity, gas, water, etc.) and manufacturing firms rely heavily on computer systems, networks, control systems, and embedded devices in order to provide safe and reliable operations. These networks can be very complex and are often bespoke to the types of product the industries may provide. In recent years we have seen a significant rise in malicious attacks against such systems, ranging from sophisticated intelligent attacks to simple tool based delivery mechanisms. With the rise in the reliance on industrial control networks and of course the increasing attacks, the lack of security monitoring and post forensic analysis of SCADA networks is becoming increasingly apparent. SCADA systems forensics is not like standard enterprise file-system forensics, the forensic specialist often has to be an expert in such systems/networks and SCADA related devices in order to identify where potential Forensic evidence could be located. This paper looks at the SCADA/industrial control systems, typical attacks and vulnerabilities, problems with forensic analysis and the development of a forensic methodology/toolkit for such systems.
Patients at High Risk for Venous Thromboembolic Events Meta-Regression Analysis to Indirectly Compare Prophylaxis With Dalteparin or Enoxaparin in

Background: Dalteparin and enoxaparin are recommended as thromboprophylaxis for at least 10 days in patients undergoing abdominal surgery (AS) or hospitalized patients with acute medical illnesses. Even though both agents have proven clinical effectiveness through randomized trials, there have been no head-to-head studies. In this evaluation, indirect statistical techniques were used to compare safety and efficacy between dalteparin and enoxaparin in these 2 high-risk patient populations. Methods: A literature search was conducted from January 1980 to November 2010 for randomized trials evaluating dalteparin or enoxaparin prophylaxis following AS or in hospitalized patients. Binary outcomes for safety and efficacy were statistically pooled using fixed or random effects models in cases of significant heterogeneity. In trials where a common control was used (eg, unfractionated heparin [UH]), indirect statistical comparisons between dalteparin and enoxaparin were performed using meta-regression analysis with active drug as the primary independent variable. Results: The meta-analysis in AS patients showed that enoxaparin or dalteparin had comparable efficacy to UH in terms of venous thromboembolic events (VTEs; relative risk reduction [RR] 1⁄4 0.87, P1⁄4 .46). The indirect statistical comparison was unable to find significant differences between enoxaparin and dalteparin in terms of risk for VTE (P 1⁄4 .84), major bleeding (P 1⁄4 .38), heparin-induced thrombocytopenia ([HIT]; P 1⁄4 .084), or death (P 1⁄4 .97). In acutely ill medical patients, treatment with enoxaparin or dalteparin had a 52% VTE risk reduction compared to placebo (RR 1⁄4 0.48, P < .001). The indirect comparison was also unable to find significant differences between enoxaparin and dalteparin in terms of VTEs (P1⁄4 .15), major bleeds (P 1⁄4 .39), HIT (P1⁄4 .48), and death (P1⁄4 .41). Conclusions: The findings suggest comparable safety and efficacy between dalteparin and enoxaparin in AS and in acutely ill medical patients.
, ADHD and Borderline Personality Disorder Comparing Dimensional Models Assessing Personality Traits and Personality Pathology Among Adult

Objective: This pilot study was a comparison of dimensional models assessing personality traits and personality pathology in a clinical sample of adults diagnosed with ADHD and adults diagnosed with borderline personality disorder (BPD), and a nonclinical control sample of healthy adults. Method: Personality traits were assessed using the NEO–Personality Inventory– Revised (NEO-PI-R) and dimensional personality pathology with the Dimensional Assessment of Personality Pathology–Basic Questionnaire (DAPP-BQ). Results: Adults with ADHD and BPD produced higher Emotional Dysregulation/Neuroticism and Dissocial Behavior scores than controls. For the Extraversion/Inhibitedness scale, adults with BPD produced significantly lower scores than adults with ADHD and controls. On the Conscientiousness/Compulsivity domains, Conscientiousness scores were lower for both disorders, whereas low Compulsivity values were specific to adult ADHD. Conclusion: Our results suggest that patients with adult ADHD and BPD have distinguishable profiles of personality traits and personality pathology. (J. of Att. Dis. 2012; XX(X) 1-XX)
Estimating Changing Volatility in Cash Flow Simulation Based Real Option Valuation with Regression Sum of Squares Error Method

This paper presents a practical volatility estimation method for cash flow simulation based real option valuation with changing volatility. During cash flow simulation, present value of the future cash flows and their corresponding cash flow state variable values are recorded for all time periods. Then, for each time period, regression analysis is used for relating the present value to the cash flow state variables of the same time period. Each regression equation provides an estimate of the expected present value as a function conditioned on the resolution of all uncertainties up to that time. Then, basic regression statistics of Pearson’s correlation R2 and sum of squares error (or standard error) for each equation provide all the information required for estimating how the standard deviation and volatility of the stochastic process change over time. The method is computationally efficient because it requires only one pass of simulation runs regardless of the number of time periods. It can also handle negative underlying asset values. Straightforward calculation of required regression statistics and their availability in any statistical software and even in spreadsheet programs make this approach very easy to apply for a practitioner.
Anran Wang Cells That Can Self-Renew After Adoptive Transfer in Humans mRNA Defines Memory T γ The Stoichiometric Production of IL-2 and IFN

destroy, cancer. -derived cells can not only seek out, but also successfully CM need to be performed to determine whether these T already being used in the clinic, and future studies combining transfer of these persistent cells with the available drugs engrafted cells expressed high levels of inhibitory receptors. Drugs that block some of these inhibitory receptors are the number is small, the authors did not observe tumor regression with this therapy. Subsequent analysis showed that characteristics. However, although the patient CM skin melanocytes but also persisted long term and reacquired T into lytic effector cells, and transferred into five patients with metastatic melanoma. These cells not only trafficked to phenotype, then expanded, differentiated CM interleukin-2. The resulting cell population was confirmed to express a T and γ interferon−− manner. The authors sorted antigen-stimulated T cells on the basis of the ratio of two cytokines need to be identified and isolated in a high-throughput CM For transplant to be practical at the clinical level, T -derived cells into human patients. CM T now translate these studies by transferring melanoma-specific et al. able to survive long term after transplant. Wang ) are CM of the transferred T cells to persist and engraft after transplant. In animal studies, central memory T cells (T tumors but not healthy tissue into patients. Early trials suggest that the success of this therapy depends on the ability Researchers have long hoped to leverage these cells to treat cancer by transferring cells that specifically target deadly assassins that seek out and destroy invading organisms. −− Antigen-specific T cells are targeted killers The Right Cells for the Job
Design of Oil Refineries Hydrogen Network Using Process Integration Principles

This paper describes the application of process integration principles to the design of oil refineries hydrogen network. In this regard, a design hierarchy as well as heuristics and required guidelines are proposed. The recommended rules compensate lack of procedure to the design and make the design process easier. The guiding principles of the design are based upon pinch technology and extending the heat integration concepts to mass integration. This research makes a designer able to maximise the amount of hydrogen recovered across the site during the design. Besides, it provides an opportunity for refineries to make most efficient use of hydrogen. The study is illustrated with an industrial case study. The work stages such as targeting, simulation etc. are performed in the REFOPT software environment. It is finally shown that this approach can design a network, which saves the total cost by $ 6.3459 million per year.
Progressive Fracture of Fiber Composite Build-Up Structures

Damage progression and fracture of built-up composite structures is evaluated by using computational simulation. The objective is to examine the behavior and response of a stiffened composite (0/ ± 45/90)S6 laminate panel by simulating the damage initiation, growth, accumulation, progression and propagation to structural collapse. An integrated computer code (CODSTRAN was augmented for the simulation of the progressive damage and fracture of built-up composite structures under mechanical loading. Results show that damage initiation and progression to have significant effect on the structural response. Influence of the type of loading is investigated on the damage initiation, propagation and final fracture of the build-up composite panel.
A study on occupational stress among physical education teachers

The performance of physical education teachers depends on various factors. One such factor is Stress that they are going the Rough work. Numerous factors affect the teacher’s Stress and this in turn affects their college life and personal life. Many institutions are not particular to provide any measures to reduce Stress. It has been identified that no study has been conducted so far about Stress management among Physical education teachers. Thus this study is devoted towards finding various causes of Stress and its consequences on Physical education teachers and the organisation in Kozhikode district of Kerala. The study also attempts to propose some suggestions to manage Stress.
, Other Protease Inhibitors of Clinical Isolates Highly Resistant to All Patterns : an Independent Cohort Analysis and Virtual Phenotypic Resistance Resistance Using Genotypic , Phenotypic , Predicting Tipranavir and Darunavir

Published Ahead of Print 5 April 2010. 10.1128/AAC.00096-10. 2010, 54(6):2473. DOI: Antimicrob. Agents Chemother. Zolopa Michel Roger, Robert Shafer, Régis Cantin and Andrew Tommy Fulisma Liu, Hugues Charest, Bluma Brenner, Annie Talbot, Philip Grant, Jonathan Taylor, Jean-Guy Baril, Other Protease Inhibitors of Clinical Isolates Highly Resistant to All Patterns: an Independent Cohort Analysis and Virtual Phenotypic Resistance Resistance Using Genotypic, Phenotypic, Predicting Tipranavir and Darunavir
Effect of a silver nanoparticles solution on staphylococcus aureus and candida spp.

An AgNPs solution was synthesized by chemical reduction, characterized, and tested against Candida glabrata, Candida tropicalis, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA). Minimum inhibitory (MICs) and minimum fungicidal/bactericidal concentrations (MFC/MBC) were determined on planktonic cells. Also, total biofilm mass was determined by crystal violet (CV) staining and morphological changes by scanning electron microscope (SEM). MICs for C. glabrata, C. tropicalis, S. aureus, and MRSA were 15.63, 3.91, 1.95, and 1.95 µg/mL, respectively. MFC for C. glabrata was 62.5 µg/mL and for C. tropicalis 15.63 µg/mL The same MBC (3.91 µg/mL) was observed for S. aureus and MRSA. CV assay showed that the AgNPs (1000 µg/mL) promoted reductions in biofilm mass of ∼60% for C. glabrata and ∼35% for C. tropicalis. A reduction of ∼20% in C. tropicalis biomass was also observed at the concentration of 3.91 µg/mL. No significant effect on total biomass was found for S. aureus and MRSA. SEM images revealed that C. glabrata and C. tropicalis biofilm cells, exposed to the AgNPs (1000 µg/mL), had an irregular and shriveled appearance. AgNPs solution exhibited considerable antimicrobial activity against important fungal and bacterial pathogens, associated with several oral and systemic diseases, and has potential as an antimicrobial agent.
Effects of electroacupuncture on the arterial stiffness in stroke patients

[Objective] It seems that in Japan’s aging society, investigating the position of acupuncture and moxibustion for the treatment of arteriosclerotic diseases is important. In this report we evaluated the effects of electroacupuncture (EA) on stroke patients by measuring changes in pulse wave velocity (PWV), brachial-ankle PWV (baPWV), ankle brachial pressure index (ABI), blood pressure (BP) and heart rate (HR). [Method] The subjects were 210 initial stroke patients. Stroke patients were randomly allocated to three groups, those only taking drug therapy (drug group), those taking drug therapy combined with rehabilitation (rehab group), and those taking drug therapy and rehabilitation combined with EA (EA group). 81 cerebral thrombosis patients (drug group n = 25, rehab group n = 28, EA group n = 28), 68 cerebral embolus patients (drug group n = 24, rehab group n = 20, EA group n = 24) and 61 cerebral hemorrhage patients (drug group n = 20, rehab group n = 21, EA group n = 20) were examined. For the evaluation method blood pressure pulse wave measuring equipment was used to measure PWV, baPWV, ABI, BP, and HR after two months, four months, and six months from the onset of treatment. [Results] After two months and four months there was no significant difference in blood vessel elasticity of the three cerebral thrombosis groups. Whereas after six months, compared to the drug group, the baPWV in cerebral thrombosis patients significantly decreased for the rehab group (P < 0.05) and EA group (P < 0.01). There were no significant differences in ABI, BP, or HR. Cerebral hemorrhage and cerebral embolism patients showed no significant difference in baPWV, ABI, BP, and HR. [Conclusion] We examined the effects of adding EA to the treatment of patients who suffered cerebral thrombosis, cerebral embolism, or cerebral hemorrhage. These results show that adding EA to drug treatment and rehabilitation significantly improves blood vessel elasticity and suggests that using EA would reduce the risk of cerebral thrombosis and its reoccurrence.
A Rank-( L , L , 1) BCD Based AOA-Polarization Joint Estimation Algorithm for Electromagnetic Vector Sensor Array

This paper investigates an angle of arrival (AOA) and polarization joint estimation algorithm for an L-shaped electromagnetic vector sensor array based on rank-(L,L, 1) block component decomposition (BCD) tensor modeling. The proposed algorithm can take full advantage of the multidimensional information of electromagnetic signal to obtain the parameter estimation more accurately than the matrix-based method and the existing tensor decomposition method. In addition, the algorithm can accomplish pair-matching of estimated parameters automatically. The numerical experiments demonstrate that even under the conditions of low SNR and limited snapshots, the proposed algorithm can still steadily achieve high detection probability with low estimation error, which is important for practical applications.
, Meilin Yao and Wenfan Yan basic psychological need satisfaction Materialism and well-being among Chinese college students : The mediating role of

Based on self-determination theory, this study explored the potential mediating role of basic psychological need satisfaction in the relationship between materialism and well-being among Chinese college students. The results showed that basic psychological need satisfaction partially mediated the relationship between materialism and life satisfaction and fully mediated the relationships among materialism and emotional wellbeing, subjective vitality, and self-actualization. The findings indicated the importance of considering both subjective and psychological well-being and the interpretative power of basic psychological need satisfaction and Chinese culture in the flow from materialism to well-being.