Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular identification of Enterobius vermicularis worms isolated from children in Babylon province of Iraq

Background and Objective: The conventional PCR technique was using for study genetic variations for Cytochrome C oxidase subunit 1 (cox1) gene of E. vermicularis between local and global to NCBI-BLAST E. vermicularis. Materials and methods: Stool samples were collected from children at early morning then transported to the laboratory. The DNA cox1 gene were isolated from stool samples then amplified by PCR technique and its results were analysis via agarose gel electrophoresis. The DNA sequencing method was carried out by sent PCR products to Macrogen Company in Korea. Phylogenetic analysis was conducted by using (UPGMA tree) method in (MEGA 6.0 version). Results: Results appears that Stool samples was positive for infection with E. vermicularis worm, DNA for cox1 gene was amplified to 407 bp that was positive at agarose gel electrophoresis. The human E. vermicularis local isolates showed homology identity to the NCBI-BLAST E. vermicularis human isolates from Iran.The phylogenetic analysis was accomplished to constructing the phylogenetic tree. The sequences of local isolates were showed have genetic relationship to NCBI-BLAST E. vermicularis with total genetic variations (0.0080-0.0020). Conclusion: PCR technique is valuable technique for isolate and know genetic variations E. vermicularis COX1 DNA genes.
One Host-Multiple Applications: Zebrafish (Danio rerio) as Promising Model for Studying Human Cancers and Pathogenic Diseases

In recent years, zebrafish (ZF) has been increasingly applied as a model in human disease studies, with a particular focus on cancer. A number of advantages make it an attractive alternative for mice widely used so far. Due to the many advantages of zebrafish, modifications can be based on different mechanisms and the induction of human disease can take different forms depending on the research goal. Genetic manipulation, tumor transplantation, or injection of the pathogen are only a few examples of using ZF as a model. Most of the studies are conducted in order to understand the disease mechanism, monitor disease progression, test new or alternative therapies, and select the best treatment. The transplantation of cancer cells derived from patients enables the development of personalized medicine. To better mimic a patient’s body environment, immune-deficient models (SCID) have been developed. A lower immune response is mostly generated by genetic manipulation but also by irradiation or dexamethasone treatment. For many studies, using SCID provides a better chance to avoid cancer cell rejection. In this review, we describe the main directions of using ZF in research, explain why and how zebrafish can be used as a model, what kind of limitations will be met and how to overcome them. We collected recent achievements in this field, indicating promising perspectives for the future.
Sociodemographics of Formal and Informal Sector Women Workers of Pakistan and a Comparison of their Perceived and Practiced Autonomy

The majority of Pakistani women are employed in the informal sector of the economy, devoid of protection, safety, and employment benefits, which prevents them from having autonomy. In this study, we aimed to identify (i) the differences in socio-demographic characteristics of formal versus informal sector women workers and (ii) compare the perceived and practised autonomy between the two groups. We used a convenience sample of 150 women, half each from the informal and formal sectors of the economy, specifically the teaching sector. We were able to find through mean analysis that women from the informal sector have higher autonomy. We also found that informal sector women workers in the country are impoverished, illiterate, non-Muslims, living in underdeveloped areas, and have no property ownership. There is a critical need to support policy for transfer to formal sector employment and to develop interventions to improve autonomy in working women.
Moderat Islamic Da’wah to Eliminate Islamophobia

Islamophobia is one of the latent problems in many moslem country, including where the majority of the population is Muslim, such as Indonesia. Hatred and fear of Islam, can disturb the harmony in society. This article describes the dangers of Islamophobia, and some strategies which can be taken to handle it. The aims of this study are to explore what are the challenges and opportunities of the Indonesian people regarding these Islamophobia problems. This article uses the theory and concept of Islamophobia. In addition, the concept of discourse popularized by Jurgen Habermas is used to examine the problems. This qualitative study uses a literature approach. Based on the discussion conducted, Islamophobia in Indonesia can permeate the personalities of non-Muslims and Muslims alike. Therefore, efforts are needed to spread an understanding of the true teachings of Islam. Such efforts are the responsibility of all Muslims. One of the ways that can be taken, is echoing Islam rahmatan lil'alamin, spoken and written at every opportunity. Most importantly, practice behavior according to the noble values ​​of Islam in order to represent the goodness of Islam in front of the widest public. Keywords: Indonesia, Islam, Islamophobia, compassion, discourse
Phylotypic Profiling, Distribution of Pathogenicity Island Markers, and Antimicrobial Susceptibility of Escherichia coli Isolated from Retail Chicken Meat and Humans

Escherichia coli (E.coli) found in retail chicken meat could be causing a wide range of infections in humans and constitute a potential risk. This study aimed to evaluate 60 E. coli isolates from retail chicken meat (n = 34) and human urinary tract infections (UTIs, n = 26) for phylogenetic diversity, presence of pathogenicity island (PAI) markers, antimicrobial susceptibility phenotypes, and antimicrobial resistance genes, and to evaluate their biofilm formation capacity. In that context, confirmed E.coli isolates were subjected to phylogrouping analysis using triplex PCR, antimicrobial susceptibility testing using the Kirby–Bauer disc diffusion method; PAI distribution was investigated by using two multiplex PCRs. Most of the chicken isolates (22/34, 64.7%) were identified as commensal E. coli (A and B1), while 12 isolates (35.3%) were classified as pathogenic virulent E. coli (B2 and D). Similarly, the commensal group dominated in human isolates. Overall, 23 PAIs were detected in the chicken isolates; among them, 39.1% (9/23) were assigned to group B1, 34.8% (8/23) to group A, 4.34% (1/23) to group B2, and 21.7% (5/23) to group D. However, 25 PAIs were identified from the human isolates. PAI IV536 was the most prevalent (55.9%, 69.2%) PAI detected in both sources. In total, 37 (61.7%) isolates of the chicken and human isolates were biofilm producers. Noticeably, 100% of E. coli isolates were resistant to penicillin and rifamycin. Markedly, all E. coli isolates displayed multiple antibiotic resistance (MAR) phenotypes, and the multiple antibiotic resistance index (MARI) among E. coli isolates ranged between 0.5 and 1. Several antibiotic resistance genes (ARGs) were identified by a PCR assay; the sul2 gene was the most prevalent (38/60, 63.3%) from both sources. Interestingly, a significant positive association (r = 0.31) between biofilm production and resistance to quinolones by the qnr gene was found by the correlation analysis. These findings were suggestive of the transmission of PAI markers and antibiotic resistance genes from poultry to humans or humans to humans through the food chain. To avoid the spread of virulent and multidrug-resistant E. coli, intensive surveillance of retail chicken meat markets is required.
The heat equation shrinks embedded plane curves to round points

Soit C(•,0):S 1 →R 2 une courbe lisse plongee dans le plan. Alors C:S 1 ×[0,T)→R 2 existe en satisfaisant δC/δt=K•N, ou K est la courbure de C, et N est son vecteur unite normal entrant. C(•,t) est lisse pour tout t, il converge vers un point quand t\T et sa forme limite quand t→T est un cercle rond, avec convergence dans norme C ∞
Dealing with COVID-19 stressful events, stress and anxiety: the mediating role of emotion regulation strategies in Italian women

BACKGROUND This study aims to better understand the causal relationship between COVID-19 stressful events, perceived stress, emotion regulation strategies and anxiety symptoms in Italian women. Specifically, we assumed that: (i) different COVID-19 stressful events can directly or indirectly influence the manifestation of anxiety symptoms; (ii) perceived stress and emotion regulation strategies can mediate the relationship between COVID-19 stressful events and anxiety symptoms. PARTICIPANTS AND PROCEDURE An online survey was distributed during the Italian mandatory lockdown – between 18th and 28th April 2020 – across the national territory. The final sample was composed of 1132 women living in different Italian regions (North 63.30%, Centre 14.20%, South 18.50%, Islands 4.00%) with a mean age of 40.19 years, ranging from 19 to 83 years (SD = 12.87). Participants filled out the Perceived Stress Scale (PSS-10), Spielberger State-Trait Anxiety Inventory (STAI) X1/R, Emotion Regulation Questionnaire (ERQ-10), five dichotomous questions assessing the COVID-19 stressful events and a demographic form. Pearson correlation analyses were performed to examine whether associations between factors conformed to the prerequisites for path analysis. Path analysis was conducted to test the model. RESULTS “Having contracted the flu during the COVID-19 pandemic” and “having a family member infected by COVID-19” have a direct effect on the level of perceived stress and an indirect effect on the manifestation of anxiety symptoms. Finally, we found that emotion regulation strategies mediate the relationship between perceived stress and state anxiety symptoms. CONCLUSIONS Our results allow one to select the stressful events to which, in the pandemic era, it is necessary to pay particular attention in a clinical setting and suggest the implementation of psychological interventions that make emotion regulation an essential direct target of treatment in chronic stress-related pathology such as anxiety disorders.
The BET Protein Inhibitor JQ1 Decreases Hypoxia and Improves the Therapeutic Benefit of Anti-PD-1 in a High-Risk Neuroblastoma Mouse Model

Anti-programmed death 1 (PD-1) is a revolutionary treatment for many cancers. The response to anti-PD-1 relies on several properties of tumor and immune cells, including the expression of PD-L1 and PD-1. Despite the impressive clinical benefit achieved with anti-PD-1 in several cancers in adults, the use of this therapy for high-risk neuroblastoma remains modest. Here, we evaluated the therapeutic benefit of anti-PD-1 in combination with JQ1 in a highly relevant TH-MYCN neuroblastoma transgenic mouse model. JQ1 is a small molecule inhibitor of the extra-terminal domain (BET) family of bromodomain proteins, competitively binding to bromodomains. Using several neuroblastoma cell lines in vitro, we showed that JQ1 inhibited hypoxia-dependent induction of HIF-1α and decreased the expression of the well-known HIF-1α downstream target gene CA9. Using MRI relaxometry performed on TH-MYCN tumor-bearing mice, we showed that JQ1 decreases R2* in tumors, a parameter associated with intra-tumor hypoxia in pre-clinical settings. Decreasing hypoxia by JQ1 was associated with improved blood vessel quality and integrity, as revealed by CD31 and αSMA staining on tumor sections. By analyzing the immune landscape of TH-MYCN tumors in mice, we found that JQ1 had no major impact on infiltrating immune cells into the tumor microenvironment but significantly increased the percentage of CD8+ PD-1+, conventional CD4+ PD-1+, and Treg PD-1+ cells. While anti-PD-1 monotherapy did not affect TH-MYCN tumor growth, we showed that combinatorial therapy associating JQ1 significantly decreased the tumor volume and improved the therapeutic benefit of anti-PD-1. This study provided the pre-clinical proof of concept needed to establish a new combination immunotherapy approach that may create tremendous enthusiasm for treating high-risk childhood neuroblastoma.
Diagnostic Assessment of Deep Learning Algorithms for Frozen Tissue Section Analysis in Women with Breast Cancer

Purpose Assessing the metastasis status of the sentinel lymph nodes (SLNs) for hematoxylin and eosin–stained frozen tissue sections by pathologists is an essential but tedious and time-consuming task that contributes to accurate breast cancer staging. This study aimed to review a challenge competition (HeLP 2019) for the development of automated solutions for classifying the metastasis status of breast cancer patients. Materials and Methods A total of 524 digital slides were obtained from frozen SLN sections: 297 (56.7%) from Asan Medical Center (AMC) and 227 (43.4%) from Seoul National University Bundang Hospital (SNUBH), South Korea. The slides were divided into training, development, and validation sets, where the development set comprised slides from both institutions and training and validation set included slides from only AMC and SNUBH, respectively. The algorithms were assessed for area under the receiver operating characteristic curve (AUC) and measurement of the longest metastatic tumor diameter. The final total scores were calculated as the mean of the two metrics, and the three teams with AUC values greater than 0.500 were selected for review and analysis in this study. Results The top three teams showed AUC values of 0.891, 0.809, and 0.736 and major axis prediction scores of 0.525, 0.459, and 0.387 for the validation set. The major factor that lowered the diagnostic accuracy was micro-metastasis. Conclusion In this challenge competition, accurate deep learning algorithms were developed that can be helpful for making a diagnosis on intraoperative SLN biopsy. The clinical utility of this approach was evaluated by including an external validation set from SNUBH.
On the decay of the magnetic fields of single radio pulsars

We investigate the statistical evidence for the decay of the magnetic field of single radio pulsars. We perform population syntheses for different assumed values for the time scale of field decay using a Monte Carlo method. We allow for the selection effects in pulsar surveys and compare the synthesized populations with the observed pulsars. We take account of the finite scale height of the distribution in the Galaxy of free electrons, which determine the dispersion measure and hence the apparent distance of radio pulsars. Our simulations give much better agreement with the observations if the time scale for the field decay is assumed to be longer than the typical active life time of a radio pulsar. This indicates that no significant field decay occurs in single radio pulsars.
On Interpretations of Hubble's Law and the Bending of Light

Currently, Hubble’s law is often considered as the observational evidence of an expanding universe. It is shown that Hubble’s Law need not be related to the notion of Doppler redshifts of the light from receding Galaxies. In the derivation of the receding velocity, an implicit assumption, which implies no expansion, must be used. Moreover, the notion of receding velocity is incompatible with the local light speeds used in deriving the light bending. The notion of an expanding universe is based on an unverified assumption that a local distance in a physical space is similar to that of a mathematical Riemannian space embedded in a higher dimensional flat space, and thus the physical meaning of coordinates would necessarily depend on the metric. However, this assumption has been proven as theoretically invalid. In fact, a physical space necessarily has a frame of reference, which has a Euclidean-like structure that is independent of the yet to be determined physical metric and thus cannot be such an embedded space. In conclusion, the notion of an expanding universe could be just a mathematical illusion.
Research Progress on Nanoparticles-Based CRISPR/Cas9 System for Targeted Therapy of Tumors

Cancer is a genetic mutation disease that seriously endangers the health and life of all human beings. As one of the most amazing academic achievements in the past decade, CRISPR/Cas9 technology has been sought after by many researchers due to its powerful gene editing capability. CRISPR/Cas9 technology shows great potential in oncology, and has become one of the most promising technologies for cancer genome-editing therapeutics. However, its efficiency and the safety issues of in vivo gene editing severely limit its widespread application. Therefore, developing a suitable delivery method for the CRISPR/Cas9 system is an urgent problem to be solved at present. Rapid advances in nanomedicine suggest nanoparticles could be a viable option. In this review, we summarize the latest research on the potential use of nanoparticle-based CRISPR/Cas9 systems in cancer therapeutics, in order to further their clinical application. We hope that this review will provide a novel insight into the CRISPR/Cas9 system and offer guidance for nanocarrier designs that will enable its use in cancer clinical applications.
Biosynthesis of Silver Nanoparticles Using Seasonal Samples of Sonoran Desert Propolis: Evaluation of Its Antibacterial Activity against Clinical Isolates of Multi-Drug Resistant Bacteria

Multi-drug resistant (MDR) bacteria have gained importance as a health problem worldwide, and novel antibacterial agents are needed to combat them. Silver nanoparticles (AgNPs) have been studied as a potent antimicrobial agent, capable of countering MDR bacteria; nevertheless, their conventional synthesis methods can produce cytotoxicity and environmental hazards. Biosynthesis of silver nanoparticles has emerged as an alternative to reduce the cytotoxic and environmental problems derived from their chemical synthesis, using natural products as a reducing and stabilizing agent. Sonoran Desert propolis (SP) is a poplar-type propolis rich in polyphenolic compounds with remarkable biological activities, such as being antioxidant, antiproliferative, and antimicrobial, and is a suitable candidate for synthesis of AgNPs. In this study, we synthesized AgNPs using SP methanolic extract (SP-AgNPs) and evaluated the reduction capacity of their seasonal samples and main chemical constituents. Their cytotoxicity against mammalian cell lines and antibacterial activity against multi-drug resistant bacteria were assessed. Quercetin and galangin showed the best-reduction capacity for synthesizing AgNPs, as well as the seasonal sample from winter (SPw-AgNPs). The SPw-AgNPs had a mean size of around 16.5 ± 5.3 nm, were stable in different culture media, and the presence of propolis constituents was confirmed by FT-IR and HPLC assays. The SPw-AgNPs were non-cytotoxic to ARPE-19 and HeLa cell lines and presented remarkable antibacterial and antibiofilm activity against multi-drug resistant clinical isolates, with E. coli 34 and ATCC 25922 being the most susceptible (MBC = 25 μg/mL), followed by E. coli 2, 29, 37 and PNG (MBC = 50 μg/mL), and finally E. coli 37 and S. aureus ATCC 25923 (MBC = 100 μg/mL). These results demonstrated the efficacy of SP as a reducing and stabilizing agent for synthesis of AgNPs and their capacity as an antibacterial agent.
Sequestration of Inflammation in Parkinson’s Disease via Stem Cell Therapy

Parkinson’s disease is the second most common neurodegenerative disease. Insidious and progressive, this disorder is secondary to the gradual loss of dopaminergic signaling and worsening neuroinflammation, affecting patients’ motor capabilities. Gold standard treatment includes exogenous dopamine therapy in the form of levodopa–carbidopa, or surgical intervention with a deep brain stimulator to the subcortical basal ganglia. Unfortunately, these therapies may ironically exacerbate the already pro-inflammatory environment. An alternative approach may involve cell-based therapies. Cell-based therapies, whether endogenous or exogenous, often have anti-inflammatory properties. Alternative strategies, such as exercise and diet modifications, also appear to play a significant role in facilitating endogenous and exogenous stem cells to induce an anti-inflammatory response, and thus are of unique interest to neuroinflammatory conditions including Parkinson’s disease. Treating patients with current gold standard therapeutics and adding adjuvant stem cell therapy, alongside the aforementioned lifestyle modifications, may ideally sequester inflammation and thus halt neurodegeneration.
Aerobic Capacity and Restitution Efficiency Level in Relation to the Training Experience and Weekly Training Volume of Male and Female Judo National Team Members in the Cadet Age Group (U18) during the Preparatory Period

This study aims to analyze the relationship between the level of aerobic capacity and post-exercise restitution during the preparation period, with training experience and weekly training volume. All (12) athletes (six women; six men) participating in the training camp of the Polish national judo team in the cadet age group were tested. The Maximal Multistage 20-m Shuttle Run Test was used to investigate the level of aerobic capacity, and the Klonowicz coefficient of restitution (COR) 3 min after exercise (COR 3′) and 5 min after exercise (COR 5′) was used to determine the level of post-exercise restitution efficiency. The results showed that higher training experience significantly affected the deterioration of COR 3′ in female athletes and improved the results in the capacity test of male athletes. Female and male athletes with a higher weekly training volume had a higher HR at the end of the performance test. Considering the demonstrated correlations, special attention should be paid to matching the loads in training programs to the age of the athletes, introducing into training programs the teaching of body management during fatigue. Failing to adjust this may make participation impossible in competitions at the international level for older age groups.