Miracle-dz/UniFAR-FTUs huggingface dataset:
Enhanced endothelial cytopathogenicity induced by a cytomegalovirus strain propagated in endothelial cells

Endothelial involvement has been implicated in cytomegalovirus (CMV) infection, a source of major complications in immunosuppressed individuals (e.g., those with acquired immune deficiency syndrome [AIDS] and organ transplants). Traditionally, CMV has been grown in fibroblasts; however, propagation in these cells may alter characteristics of the virus. In developing an in vitro model system of CMV/endothelial cell interaction, we have addressed this issue by propagating a clinical isolate, CMV VHL 1, in human umbilical vein endothelial (HUVE) cells by serial cocultivation of heavily infected cultures with fresh HUVE monolayers and have compared its infectious properties with those of the fibroblast‐raised strain, CMV AD169. In situ hybridization using a biotinylated DNA probe, as well as immunofluorescent staining for CMV specific antigen, has confirmed infection of HUVE cells inoculated with either strain of the virus. Infection of HUVE by VHL was accompanied by dramatic cytopathology not observed in AD169‐infected cells. Plaque assay of culture supernatants revealed greater virus production in VHL‐infected HUVE as compared with equivalently inoculated fibroblasts. In contrast, AD169 production in inoculated fibroblasts exceeded that in HUVE. These studies demonstrate the suitability of cultured endothelial cells as a substrate for CMV propagation and suggest that a strain of virus thus propagated may offer an accurate model of CMV/endothelial cell interaction in human disease.
Psychometric Properties of the Spanish PID-5 in a Clinical and a Community Sample

The Personality Inventory for DSM-5 (PID-5) measures the trait part (Criterion B) of the alternative model for personality disorders proposed in Section III of DSM-5. Although its psychometric properties have proven adequate thus far, evidence is limited in other languages and in clinical samples. The Spanish PID-5 was examined in two samples comprising 446 clinical and 1,036 community subjects. Facet scales showed good internal consistency in both samples (median α = .86 and .79) and were unidimensional under exploratory and confirmatory approaches. They were also able to distinguish between clinical and community subjects with a mean standardized difference of z = 0.81. All facets except for Risk Taking were unipolar, such that the upper poles indicated pathology and the lower poles reflected normality, rather than the opposite pole of abnormality. The entire PID-5 hierarchical structure, from one to five factors, was confirmed in both samples with Tucker’s congruence coefficients over . 95.
Video-on-Demand Over ATM: Constant-Rate Transmission and Transport

We introduce a specific transport and transmission scheme for video-on-demand (VoD) called constant-rate transmission and transport (CRTT). CRTT establishes a constant bit-rate (CBR) virtual channel between the video provider and the viewer's set-top box (STB) and then transmits cells from the provider into this channel at a constant rate. Since we assume that the number of cells in a frame is variable, CRTT requires that some number of cells be built up in an STB buffer before the commencement of playback. The build up, cell transmission rate, and the set-top memory size must be chosen so that there is no starvation or overflow at the STB. We develop fundamental relationships between these parameters for viable CRTT. We then apply the theory to an MPEG encoding of Star Wars and find that the minimal STB memory far CRTT is 23 Mbytes. We also consider varying the constant rate over a small number of intervals. We find, for example, that for Star Wars approximately 2 Mbytes of set-top memory suffices with 32 constant-rate intervals.
Dynamics and Frequency Response of Microring-Based Optical Modulator Considering Nonlinear Effects

In this paper, we analyze dynamics and frequency response of GaAs-based microring modulators at the 1.55 μm using the finite-difference time-domain method. Two types of modulation are investigated: pump-probe configuration and modulation of the coupling coefficient. The pump-probe signals are tuned at separate resonance wavelengths of the microring. Change of effective refractive index of the microring by two-photon absorption is used to modulate the probe beam. By applying probe beam in four timing configurations, it is shown that the wavelength shift of the probe depends not only on the pump intensity but also on the interaction length over which the pump and probe pulses overlap. It is also shown that free-carrier absorption increases when the temporal width of pump signal is wide enough. Modulation bandwidth of microring in the presence of nonlinear effects is investigated and it is shown that the bandwidth is in 10 GHz range. In order to increase the bandwidth of the intensity modulator, controlled coupling is used. It is shown that in this case, modulation bandwidth of waveguide-ring coupling strength becomes three times wider.
Constructing Nested Nodal Sets for Multivariate Polynomial Interpolation

We present a robust method for choosing multivariate polynomial interpolation nodes. Our algorithm is an optimization method to greedily minimize a measure of interpolant sensitivity, a variant of a weighted Lebesgue function. Nodes are therefore chosen that tend to control oscillations in the resulting interpolant. This method can produce an arbitrary number of nodes and is not constrained by the dimension of a complete polynomial space. Our method is therefore flexible: nested nodal sets are produced in spaces of arbitrary dimensions, and the number of nodes added at each stage can be arbitrary. The algorithm produces a nodal set given a probability measure on the input space, thus parameterizing interpolants with respect to finite measures. We present examples to show that the method yields nodal sets that behave well with respect to standard interpolation diagnostics: the Lebesgue constant, the Vandermonde determinant, and the Vandermonde condition number. We also show that a nongreedy version of the .
Young children's sibling relationship quality: distal and proximal correlates.

BACKGROUND Relationships within families are interdependent and related to distal environmental factors. Low socioeconomic status (SES) and high household chaos (distal factors) have been linked to less positive marital and parent-child relationships, but have not yet been examined with regard to young children's sibling relationships. The present study tested direct associations between these distal factors and sibling relationship quality, as well as examining parenting as a potential mediator and/or moderator. METHOD One hundred and eighteen families with children aged 4 to 8 years were interviewed and completed questionnaires during home visits. Children provided reports about the quality of their sibling relationships via a puppet interview, and mothers and fathers reported on household chaos and their exertion of harsh discipline. Researchers rated parental warmth towards the children. Finally, parental education and household density were assessed as indicators of SES. RESULTS Using structural equation modelling (SEM), we found that lower levels of household chaos were related to better quality sibling relationships, and that this link was mediated by maternal warmth and paternal harsh discipline, as well as moderated by maternal harsh discipline. CONCLUSION Household chaos was more strongly linked to relationship quality between family members than SES, warranting further research and practical application. The study of children's sibling relationships should take into account inter-relations between proximal and distal environmental factors to fully reveal the complexity of family life.
Gate first high-k/metal gate stacks with zero SiOx interface achieving EOT=0.59nm for 16nm application

Gate first 0.59 nm EOT HfO<sub>x</sub>/metal gate stacks for 16 nm node application are demonstrated for the first time. By controlling O during HfO<sub>x</sub> deposition, ldquozerordquo low-k SiO<sub>x</sub> interface (ZIL) forms despite a 1020degC activation anneal. This 0.59 nm EOT is a 30% improvement over a state of the art 32 nm HK/MG technology. We compare and demonstrate for the first time the improved scalability of ZIL HfO<sub>x</sub> vs. exotic higher-k. Transistors made with ZIL HfO<sub>x</sub> show good interfaces (SS=70-80 mV/dec, N<sub>it</sub> = 5 times 10<sup>10</sup>/cm<sup>2</sup>) and performance (10% I<sub>on</sub>-I<sub>off</sub> boost vs. EOT = 0.95 nm), despite mobility loss. Factors contributing to mobility loss in ZIL HfO<sub>x</sub> are discussed.
Carboxylation of phosphoenolpyruvate by extracts of Neisseria gonorrhoeae

The enzymatic carboxylation of phosphoenolpyruvate by cell-free extracts of Neisseria gonorrhoeae was examined and determined to be similar to the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). This was shown by the irreversibility of the reaction and nucleotide independency. The enzyme was found to have some characteristics different from the other bacterial PEPCs reported. The enzyme showed catalytic activity in the presence of cobalt ions as well as magnesium and manganese ions, was not inhibited by succinate in fresh extracts, and displayed a low Michaelis constant for bicarbonate (0.27 mM), as compared with other PEPCs. The significance of this low Michaelis constant is discussed with respect to the growth of the organism and the importance of this enzyme to protein and nucleic acid synthesis.
Revealing the complex nature of the strong gravitationally lensed system H-ATLAS J090311.6+003906 using ALMA

We have modelled Atacama Large Millimetre/sub-millimetre Array (ALMA) long baseline imaging of the strong gravitational lens system H-ATLAS J090311.6+003906 (SDP.81). We have reconstructed the distribution of band 6 and 7 continuum emission in the z = 3.042 source and determined its kinematic properties by reconstructing CO(5–4) and CO(8–7) line emission in bands 4 and 6. The continuum imaging reveals a highly non-uniform distribution of dust with clumps on scales of ∼200 pc. In contrast, the CO line emission shows a relatively smooth, disc-like velocity field which is well fitted by a rotating disc model with an inclination angle of (40 ± 5)° and an asymptotic rotation velocity of 320 km s−1. The inferred dynamical mass within 1.5 kpc is (3.5 ± 0.5) × 1010 M⊙ which is comparable to the total molecular gas masses of (2.7 ± 0.5) × 1010 M⊙ and (3.5 ± 0.6) × 1010 M⊙ from the dust continuum emission and CO emission, respectively. Our new reconstruction of the lensed Hubble Space Telescope near-infrared emission shows two objects which appear to be interacting, with the rotating disc of gas and dust revealed by ALMA distinctly offset from the near-infrared emission. The clumpy nature of the dust and a low value of the Toomre parameter of Q ∼ 0.3 suggest that the disc is in a state of collapse. We estimate a star formation rate in the disc of 470 ± 80 M⊙ yr−1 with an efficiency ∼65 times greater than typical low-redshift galaxies. Our findings add to the growing body of evidence that the most infrared luminous, dust obscured galaxies in the high-redshift Universe represent a population of merger-induced starbursts.
Ureteral obstruction in cancer patients: a qualitative study

Amanika Kumar, Lance Mynderse, Krishna Patel, Megan Grudem, Jamie Bakkum-Gamez, Sherri Longenbach, Matthew S. Block, Henry C. Pitot, Vesna Garovic, S. John Weroha and Aminah Jatoi* Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, United States Department of Urology, Mayo Clinic, Rochester, MN, USA Department of Medicine, Mayo Clinic, Rochester, MN, USA Department of Oncology, Mayo Clinic, Rochester, MN, USA Aminah Jatoi, 200 First Street SW, Rochester, MN 55905, USA
A Fast Algorithm for the Minimum Covariance Determinant Estimator

The minimum covariance determinant (MCD) method of Rousseeuw is a highly robust estimator of multivariate location and scatter. Its objective is to find h observations (out of n) whose covariance matrix has the lowest determinant. Until now, applications of the MCD were hampered by the computation time of existing algorithms, which were limited to a few hundred objects in a few dimensions. We discuss two important applications of larger size, one about a production process at Philips with n = 677 objects and p = 9 variables, and a dataset from astronomy with n = 137,256 objects and p = 27 variables. To deal with such problems we have developed a new algorithm for the MCD, called FAST-MCD. The basic ideas are an inequality involving order statistics and determinants, and techniques which we call “selective iteration” and “nested extensions. ” For small datasets, FAST-MCD typically finds the exact MCD, whereas for larger datasets it gives more accurate results than existing algorithms and is faster by orders.
REAL-TIME FACE TRACKING WITH GPU ACCELERATION

Fast and robust tracking of multiple faces is receiving increased attention from computer vision researchers as it finds potential applications in many fields like video surveillance and computer mediated video conferencing. Real-time tracking of multiple faces in high resolution videos involve three basic tasks namely initialization, tracking and display. Among these, tracking is quite compute intensive as it involves particle filtering that won’t yield a real time performance if we use a conventional CPU based system alone. While looking forward a design that optimizes the system for an appreciable real-time performance, calls for the use of compute efficient platforms like GPU for compute intensive tasks along with conventional CPU. This paper discusses our heterogeneous design model which combines conventional programming for CPU efficient tasks and the nVIDIA CUDA for GP-GPU that implements the compute intensive tasks.
Unequal error protection with eigen beamforming for partial channel information MIMO-OFDM

Some communication applications, like multimedia, deliver data of different importance classes allowing unequal error protection (UEP) levels. In this paper, a multiple-input multiple-output (MIMO) system using orthogonal frequency division multiplexing (OFDM) is considered with a new UEP bit-loading algorithm based on the non-UEP algorithm by Chow, Cioffi, and Bingham. In the proposed bit-loading algorithm, bits are distributed across the unitarily transformed eigenbeams in case of partial channel information (CSI) knowledge. However, ideal performance can not withstand the rapid wireless channel variation unless a restricted beamforming is used. For the case of partial CSI, we proposed a switched beamforming technique that can maintain the required performance protection levels.
Application of ceramic piezoelectric device to audio speaker

This paper describes a new flat-panel type ceramic piezoelectric transducer for audio speakers. The proposed transducer offers a simple configuration compared to a conventional magnetically actuated transducer. The drawbacks of piezoelectric actuated transducers are: (P1). Impedance matching problem due to piezoelectric transducer; (P2) Resonance frequency problem caused by stiffness of piezoelectric transducer; (P3) Directivity problem caused by shape of panel; (P4) Amplitude problem caused by small piezoelectric vibration amplitudes. To solve (P1) and (P2), we newly developed a low frequency boost filter based on an LC resonance circuit. To solve (P3), to improve the directivity of the proposed transducer, we are actuated and measured various transducer arrangements and types of vibration plate. To solve (P4), we developed a new voltage based BTL amplifier which is suitable for piezoelectric actuators. We verified the validity of the proposed transducer by the frequency domain of white noise.
Intermediate filament proteins immunologically related to cytokeratins in the oocyte of the fish Cyprinus carpio

Summary We have used monoclonal antibodies specific for different sets of human cytokeratins and the anti-IFA (Intermediate Filament Antigen) antibody to investigate the expression of intermediate filament proteins in the mature oocyte of the teleostCyprinus carpio. Several polypeptides have been identified, showing molecular weights ranging from 43 to 65kDa. Two-dimensional analysis of the immunoreactive species revealed the presence of at least six major protein spots and a series of minor components, grouped in quite a narrow pI range from 5.52 to 6.28. The general complexity of the carp oocyte cytokeratin-related cytoskeleton appears to be higher than those described for oocytes of other vertebrate species.
Expression of Phosphatidylserine‐Dependent Antigens on the Surface of Differentiating BeWo Human Choriocarcinoma Cells

PROBLEM: Antiphospholipid antibodies (aPLs) are associated with pregnancy loss, pregnancy‐induced hypertension, and intrauterine growth retardation. We have previously reported that phosphatidylserine (PS)‐dependent antigens are expressed in formalin‐fixed cells concurrent with differentiation in a choriocarcinoma model (BeWo) of cytotrophoblast. That study, however, could not differentiate between cytoplasmic or surface antigen expression.
KRAS Mutation Testing in Human Cancers: The Pathologist's Role in the Era of Personalized Medicine

A number of studies have shown that although antiepidermal growth factor receptor (EGFR) monoclonal antibodies are effective treatments for metastatic colorectal cancer (mCRC), only patients with wild-type KRAS tumors derive clinical benefit from these therapies. The anti-EGFR monoclonal antibodies panitumumab and cetuximab are approved in the United States for treatment of mCRC refractory to chemotherapy but are not recommended for use in patients with mutations in KRAS codons 12 or 13. Similarly, panitumumab is approved for the treatment of mCRC only in patients with wild-type KRAS in Europe and Canada. It is clear that KRAS mutational analysis will become an important aspect of disease management in patients with mCRC. Consequently, it will be important for pathologists and oncologists to develop and agree on standardized KRAS testing and reporting procedures to ensure optimum patient care. Pathologists will be central to this process because of their crucial role in selecting appropriate tumor specimens for testing, choosing the molecular diagnostic laboratory to be used, assisting in the selection of a suitable KRAS test, and interpreting the results of KRAS mutational analysis. Guidelines for KRAS testing that address these and other important points of consideration have recently been proposed in the United States and the European Union.
Influence of Knee Flexion Angle and Age on Triceps Surae Muscle Fatigue During Heel Raises

Abstract Hébert-Losier, K, Schneiders, AG, García, JA, Sullivan, SJ, and Simoneau, GG. Influence of knee flexion angle and age on triceps surae muscle fatigue during heel raises. J Strength Cond Res 26(11): 3134–3147, 2012—The triceps surae (TS) muscle-tendon unit is 1 of the most commonly injured in elite and recreational athletes, with a high prevalence in middle-aged adults. The performance of maximal numbers of unilateral heel raises is used to assess, train, and rehabilitate TS endurance and conventionally prescribed in 0° knee flexion (KF) for the gastrocnemius and 45° for the soleus (SOL). However, the extent of muscle selectivity conferred through the change in the knee angle is lacking for heel raises performed to volitional fatigue. This study investigated the influence of knee angle on TS muscle fatigue during heel raises and determined whether fatigue differed between middle-aged and younger-aged adults. Forty-eight healthy individuals aged 18–25 and 35–45 years performed maximal numbers of unilateral heel raises in 0° and 45° KF. Median frequencies and linear regression slopes were calculated from the SOL, gastrocnemius medialis (GM), and gastrocnemius lateralis (GL) surface electromyographic signals. Stepwise mixed-effect regressions were used for analysis. The subjects completed an average of 45 and 48 heel raises in 0° and 45° KF, respectively. The results indicated that the 3 muscles fatigued during testing as all median frequencies decreased, and regression slopes were negative. Consistent with muscle physiology and fiber typing, fatigue was greater in the GM and GL than in the SOL (p < 0.001). However, knee angle did not influence TS muscle fatigue parameters (p = 0.814), with similar SOL, GM, and GL fatigue in 0° and 45° KF. These findings are in contrast with the traditionally described clinical use of heel raises in select knee angles for the gastrocnemius and the SOL. Furthermore, no difference in TS fatigue between the 2 age groups was able to be determined, despite the reported higher prevalence of injury in middle-aged individuals.
Phylogeography of a marine acanthocephalan: lack of cryptic diversity in a cosmopolitan parasite of mole crabs

Little is known about phylogeography and cryptic diversity of parasites in the marine environment. The acanthocephalan Profilicollis altmani parasitizes intermediate hosts that are broadly distributed around the Americas and final hosts that are highly motile. We investigated the spatial genetic structure of this acanthocephalan found in three species of Emerita crabs: (1) to test whether land masses serve as biogeographic barriers promoting ocean basin divergence among parasite lineages or species; and (2) to test whether the distribution of parasite species matches the distribution of different crab host species.
Early Detection of Bladder Dysfunction Following Posterior Urethral Valves Ablation

Urinary incontinence following Posterior Urethral Valves (PUV) ablation has been attributed in the past to sphincter injury, but it is nowadays accepted that bladder dysfunction (BD) plays a determinant role. In order to assess BD evolution, we have evaluated, from 1982 to 1994, 48 boys with PUV by urodynamics (UD) studies. Age of the patients ranged from 10 months to 15 years. A total of 65 examinations were performed. We considered four groups: 0-3 years; 4-7 years; 8-12 years and > 12 years. Uninhibited detrusor contractions (instability), end filling pressure (EFP), bladder capacity and voiding detrusor pressure were evaluated in order to assess the presence of BD distinguishing it in: hypercontractility, hypocontractility and low-compliant bladder. The results collected confirm a high percentage of BD in PUV boys (71%) and the evolution of hypercontractility versus hypocontractility in 60% of patients considered.
Patients with rectal cancer having neoadjuvant chemoradiation do not have increased complications of ileostomy closure.

OBJECTIVES It is conceivable that reversal of an ileostomy after low anterior resection following neoadjuvant therapy (NAT) may involve anastomosis of small bowel exposed to irradiation. The aim was to evaluate peri-operative complications of ileostomy closure and to compare the histology of ileal mucosa in excised stomas in patients who received NAT with those without NAT. METHODS Twenty patients who underwent rectal excision following NAT for cancer, were compared with 20 control patients who underwent rectal excision without NAT. All patients received a diverting loop ileostomy which was subsequently reversed with excision of the ileostomy. The clinical outcome and histopathological features after reversal were evaluated. RESULTS There was no significant difference with regard to peri-operative complications such as post-operative deaths related to ileostomy closure, anastomotic leakage, retraction of stoma or small bowel fistulae. Resection margins revealed no significant difference in crypt distortion, depletion of mucin, acute inflammation, chronic inflammation and infiltration of eosinophils following NAT compared with controls. CONCLUSIONS Neoadjuvant therapy for rectal cancer does not result in higher morbidity following closure of diverting loop ileostomy or result in significant inflammatory changes in the ileum. Therefore ileostomy closure is as safe in those with preoperative radiotherapy as in those without neoadjuvant therapy.