Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of visual P300 in cognitive assessment of subacute stroke patients: a longitudinal study

Abstract Aim: The aim of this study was to assess if pre- and post- event related potentials (ERPs) components after visual stimulation training admitted differences exist in subacute stroke patients and to observe the outcome markers of cognitive functions recovery by using Neurowave system. Materials and Methods: Eighteen ischemic subacute stroke patients were enrolled and underwent to neuropsychological evaluation and ERPs recording at two time points, at baseline (T0) and after 3 months from (T1). All patients were stimulated with customized images for three days a week. Findings: Intra group analysis showed a significance difference in ERPs parameters and neuropsychological tests. Correlation analysis showed a significant relation between neuropsychological and ERP values. Conclusion: Our findings confirm that P300 ERPs’ component could be a predictive marker for cognitive recovery of ischemic subacute stroke patients. Intensive programs of neurosensory stimulation could facilitate recovery of cognitive and attentive functions in subacute cerebrovascular disease.
ERK1 indicates good prognosis and inhibits breast cancer progression by suppressing YAP1 signaling

The mitogen-activated protein kinase/extracellular signal-regulated (MAPK/ERK) pathway is a well-characterized signaling pathway during the development of various cancer types. ERK1 and ERK2, the two kinase effectors of MAPK cascade, exhibit high similarity. However, it is still unknown whether these two kinases are functionally different or in contrast functionally redundant during the development of breast cancer. We found that ERK1 expression levels were significantly lower in basal breast cancer compared with luminal breast cancer and normal breast tissues. RNA sequencing data suggested that ERK1 was associated with Hippo signaling pathway and cell proliferation in breast cancer cells. The gene set enrichment analysis (GSEA) further showed enrichment for YAP1 signaling pathway in breast cancer cell lines and tumors with low expression of ERK1. Silencing of ERK1 elevated YAP1 expression and TEAD activity in breast cancer cells. Additionally, ERK1 inhibited breast cancer cell proliferation via regulation of YAP1. The Kaplan-Meier analysis of data in patients with breast cancer suggested that, higher expression of ERK1 was associated with better prognosis, whereas, higher expression of ERK2 predicted poorer prognosis. These findings unveiled the role of ERK1 on regulation of YAP1 signaling pathway, indicating ERK1 as a negative regulator of breast cancer progression.
African Multi-Site 2-Year Neuropsychological Study of School-Age Children Perinatally Infected, Exposed, and Unexposed to Human Immunodeficiency Virus.

BACKGROUND Children living with human immunodeficiency virus (HIV) are at neuropsychological risk for cognitive and motor dysfunction. However, few prospective, multi-site studies have evaluated neuropsychological outcomes longitudinally among perinatally infected African children who received early antiretroviral treatment (ART). METHODS We enrolled 611 children aged 5 to 11 years at 6 sites (South Africa [3], Zimbabwe, Malawi, Uganda). Of these, there were 246 children living with HIV (HIV+) who were initiated on ART before 3 years of age in a prior clinical trial comparing nevirapine to lopinavir/ritonavir (International Maternal Pediatric Adolescent Acquired Immunodeficiency Syndrome Clinical Trials [IMPAACT] P1060); 183 age-matched, exposed but uninfected (HEU) children; and 182 unexposed and uninfected (HUU) children. They were compared across 3 assessment time points (Weeks 0, 48, and 96) on cognitive ability (Kaufman Assessment Battery for Children, second edition [KABC-II]), attention/impulsivity (Tests of Variables of Attention [TOVA]), motor proficiency (Bruininks-Oseretsky Test, second edition [BOT-2]), and on the Behavior Rating Inventory of Executive Function (BRIEF). The cohorts were compared using linear mixed models, adjusting for site, child's age and sex, and selected personal/family control variables. RESULTS The HIV+ cohort performed significantly worse than the HEU and HUU cohorts for all KABC-II, TOVA, and BOT-2 performance outcomes across all 3 time points (P values < .001). The HUU and HEU cohorts were comparable. For the KABC-II planning/reasoning subtests, the HIV+ children showed less improvement over time than the HUU and HEU groups. The groups did not differ significantly on the BRIEF. CONCLUSIONS Despite initiation of ART in early childhood and good viral suppression at the time of enrollment, the HIV+ group had poorer neuropsychological performance over time, with the gap progressively worsening in planning/reasoning. This can be debilitating for self-management in adolescence.
An initial exploration of hair cortisol responses to antidepressants in unmedicated patients with depression

Objective Major depressive disorder is associated with abnormal functioning of the hypothalamic–pituitary–adrenal (HPA) axis. Studies using hair cortisol to measure the effect of antidepressants on the HPA axis are lacking. The aim of this study was to explore the long-term effects of antidepressants on hair cortisol concentration (HCC). Methods Participants were 42 patients and 36 healthy individuals. The patients took antidepressants for 4 weeks. Patient HCC was measured pre-treatment and post-treatment. The HCC of healthy controls was also measured. Results Patient post-treatment HCC (mean ± standard deviation: 34.40 ± 32.57 pmol/mg) was significantly higher than patient pre-treatment HCC (17.42 ± 12.40 pmol/mg) and healthy control HCC (10.22 ± 7.99 pmol/mg). No significant correlation was found between Hamilton Depression Rating Scale scores and HCC at pre-treatment or post-treatment. Conclusions Hair cortisol concentration analysis could be used to monitor the dynamics of the effects of antidepressants on the HPA axis.
The effects of hydroalcoholic extract of spinach on prevention and treatment of some metabolic and histologic features in a rat model of nonalcoholic fatty liver disease.

BACKGROUND This study evaluated the effects of hydroalcoholic extract of spinach (HES) on nonalcoholic fatty liver disease (NAFLD). In the prevention phase, 18 Sprague-Dawley rats were fed a high-fat diet, a high-fat diet plus 400mg/kg HES, or a chow diet for seven weeks. For the treatment phase, after the induction of NAFLD, they were fed a high-fat diet, a high-fat diet plus 400mg/kg HES, a chow diet, or chow diet plus 400mg/kg HES for four weeks (n=6). RESULTS Weight gain (P = 0.01), food intake (P < 0.01), serum glucose (P=0.01), TG (P=0.02), LDL-Cholesterol (P=0.01), AST (P=0.02), liver steatosis and NAS (P<0.01) in the high-fat group were statistically more than other groups at the end of the prevention phase. Feeding spinach extract to rats on a high-fat diet decreased serum glucose (P=0.01), TC (P<0.01), AST (P=0.01), ALP (P<0.01) and liver steatosis (P<0.01) in the treatment phase. CONCLUSION Overall, spinach extract showed beneficial effects in the prevention and treatment of NAFLD. This article is protected by copyright. All rights reserved.
Ear Detection Using Convolutional Neural Network on Graphs with Filter Rotation

Geometric deep learning (GDL) generalizes convolutional neural networks (CNNs) to non-Euclidean domains. In this work, a GDL technique, allowing the application of CNN on graphs, is examined. It defines convolutional filters with the use of the Gaussian mixture model (GMM). As those filters are defined in continuous space, they can be easily rotated without the need for some additional interpolation. This, in turn, allows constructing systems having rotation equivariance property. The characteristic of the proposed approach is illustrated with the problem of ear detection, which is of great importance in biometric systems enabling image based, discrete human identification. The analyzed graphs were constructed taking into account superpixels representing image content. This kind of representation has several advantages. On the one hand, it significantly reduces the amount of processed data, allowing building simpler and more effective models. On the other hand, it seems to be closer to the conscious process of human image understanding as it does not operate on millions of pixels. The contributions of the paper lie both in GDL application area extension (semantic segmentation of the images) and in the novel concept of trained filter transformations. We show that even significantly reduced information about image content and a relatively simple, in comparison with classic CNN, model (smaller number of parameters and significantly faster processing) allows obtaining detection results on the quality level similar to those reported in the literature on the UBEAR dataset. Moreover, we show experimentally that the proposed approach possesses in fact the rotation equivariance property allowing detecting rotated structures without the need for labor consuming training on all rotated and non-rotated images.
Identification and Preference of Game Styles in LaLiga Associated with Match Outcomes

The objective was to model the teams’ styles of play (SoPs) in elite football and relate them to the match result. For this, the twenty Spanish first division teams in the 2016–2017 season were analysed, using nine interaction performance indicators (IRi). A principal component (PC) analysis was applied. From two PCs four SoPs were established: deep or high-pressure defending, and elaborate or direct attack. The SoPs were distributed according to average performance obtained throughout the championship. The connection between the preferred SoP and the final result was estimated. Teams with elaborate offensive styles and teams defensively minded got better results. In addition, most of the teams showed variability in their SoP. The applications of the study are (1) the IRi have served to identify SoP and can be used as a reference to optimize team performance; (2) teams should have a varied SoP repertoire, as well as being prepared to deal with different SoPs; (3) particular player profiles should be connected with the desired SoP when creating the squad and (4) clubs should develop a varied range of SoPs at their academies.
A pipeline for multidimensional confocal analysis of mitochondrial morphology, function and dynamics in pancreatic β-cells

Mitochondrial network morphology and dynamics are inextricably linked to cellular metabolism and health, and live-cell imaging of mitochondria can therefore provide vital insights into both physiology and pathophysiology. As a notable example, mitochondria are critical for nutrient-induced insulin secretion from pancreatic β-cells, and a better understanding of their dynamics and dysregulation could shed significant light on the pathogenesis of type 2 diabetes. However, without using super-resolution microscopy and commercial analysis software it can be exceedingly challenging to accurately extract mitochondrial features in cells with dense multi-layered networks, such as β-cells. Motivated by this, we developed an analysis pipeline and ImageJ plugin that enables 2D/3D quantification of mitochondrial network morphology and dynamics in β-cells and, by extension, in other similarly challenging cell-types. Importantly, this approach is based on standard confocal microscopy and shareware, making it widely accessible. The accuracy of mitochondrial identification was established by photo-labelling individual mitochondria, and we validated the ability to quantitatively categorize mitochondrial 2D/3D shape and network descriptors using unsupervised cluster analysis. We further demonstrate the capability for analyzing mitochondrial morphology and function on a per-mitochondrion basis, as well as 4D (xyzt) analysis of mitochondrial dynamics. The pipeline also offers a convenient framework for multiplexing of imaging-based readouts of mitochondrial state/function, which we propose could facilitate expansive identification of mitochondrial “morpho-functional” fingerprints associated with various physiological and pathophysiological states. Overall, this analysis pipeline permits robust multidimensional quantification of mitochondrial morphology and dynamics, and provides a valuable resource to accelerate research into the role of mitochondria in disease.
Imaging Measurement of Whole Gut Transit Time in Paediatric and Adult Functional Gastrointestinal Disorders: A Systematic Review and Narrative Synthesis

Background: functional gastrointestinal disorders (FGID) are common conditions in children and adults, often associated with abnormalities of whole gut transit. Currently, transit tests can be performed using several imaging methods, including tracking of radiopaque markers, gamma scintigraphy with the use of radioisotopes, magnetic tracking methods, tracking of movement of wireless motility capsules, and emerging magnetic resonance imaging (MRI) approaches. Objectives: to review recent literature on diagnostic imaging techniques used to investigate whole gut transit in FGIDs. Methods: a systematic review was carried out. The different techniques are described briefly, with particular emphasis on contemporary literature and new developments, particularly in the field of MRI. Conclusions: emerging MRI capsule marker methods are promising new tools to study whole gut transit in FGIDs.
A new method for left carotid angiography: use right judkins 4 guiding (6f) catheter with 2 side holes.

OBJECTIVE To introduce a rapid and simple technique to engage and localize through left common carotid (LCCA) artery during selective carotid angiography using the same type of catheter (6F Medtronic™/launcher Judkins Right Guide Catheter, 4.0 Tip,2 Side holes, U.S.A.). METHODS Patients were divided into two groups as new method and conventional method group including 295 and 292 prior to carotid angiography, respectively. The primary endpoints of this study included an analysis of total procedure time, time required for cannulation of left common carotid artery, fluoroscopy time and selective imaging success for left common carotid artery, feasibility, safety, amount of radio opaque agent and radiation exposure. RESULTS Our novel method applied using a single catheter shortened total procedure time (5.41 ± 1.56 min. vs. 7.52 ± 2.48 min., respectively, p<0.001), fluoroscopy time (98 ± 9 sec. vs. 210 ± 19 sec., respectively, p<0.001), duration of left carotid artery cannulation (15 ± 1.2 sec vs. 42 ± 1.9 sec, respectively, p< 0.001). Use of our method reduced radiation exposure (120 ± 17 mGy vs. 217 ± 11 mGy, respectively, p<0.001), amount of contrast agent (44.99 ± 12.84 ml vs. 59.89 ± 18.93 ml, respectively, p<0.001). Aortic arch angiography was not needed in the first group compared with the conventional method group (0% vs. 29%, respectively, p<0.001). Also a higher success was achieved in cannulation of left common carotid artery using an identical catheter compared with application of conventional methods requiring use of different types of catheter (100% vs. 60%, respectively, p<0.001). No major complication and mortality was found due to use of both methods. However, a statistically insignificant elevation in minor complication was encountered in the patient group who underwent coronary angiography by the conventional method (3% vs. 7%, respectively, p<0.064). CONCLUSIONS This new method is more easily applicable with a shorter duration for cannulation and localization of LCCA, safer for the patients with use of a limited amount of radio-opaque agent and finally cost-effective by requiring only one type of catheter and a limited.
Use of whole genome sequencing of commensal Escherichia coli in pigs for antimicrobial resistance surveillance, United Kingdom, 2018

Background Surveillance of commensal Escherichia coli, a possible reservoir of antimicrobial resistance (AMR) genes, is important as they pose a risk to human and animal health. Most surveillance activities rely on phenotypic characterisation, but whole genome sequencing (WGS) presents an alternative. Aim In this retrospective study, we tested 515 E. coli isolated from pigs to evaluate the use of WGS to predict resistance phenotype. Methods Minimum inhibitory concentration (MIC) was determined for nine antimicrobials of clinical and veterinary importance. Deviation from wild-type, fully-susceptible MIC was assessed using European Committee on Antimicrobial Susceptibility Testing (EUCAST) epidemiological cut-off (ECOFF) values. Presence of AMR genes and mutations were determined using APHA SeqFinder. Statistical two-by-two table analysis and Cohen’s kappa (k) test were applied to assess genotype and phenotype concordance. Results Overall, correlation of WGS with susceptibility to the nine antimicrobials was 98.9% for test specificity, and 97.5% for the positive predictive value of a test. The overall kappa score (k = 0.914) indicated AMR gene presence was highly predictive of reduced susceptibility and showed excellent correlation with MIC. However, there was variation for each antimicrobial; five showed excellent correlation; four very good and one moderate. Suggested ECOFF adjustments increased concordance between genotypic data and kappa values for four antimicrobials. Conclusion WGS is a powerful tool for accurately predicting AMR that can be used for national surveillance purposes. Additionally, it can detect resistance genes from a wider panel of antimicrobials whose phenotypes are currently not monitored but may be of importance in the future.
Smoking and social anxiety: the role of false safety behaviors

ABSTRACT Despite the negative health consequences associated with smoking, most smokers find it difficult to quit. This is especially true for smokers with elevated social anxiety. One factor that may play a role in maintaining smoking with elevated anxiety is false safety behavior (FSB), behaviors geared toward decreasing anxiety short-term but that maintain or increase anxiety long-term. The present study tested whether FSB explained the relation of social anxiety severity with smoking among 71 current smokers. Avoidance-related FSB was the only type of FSB related to cigarettes smoked per day (CPD) and it was robustly related to more CPD. Further, social anxiety was related to CPD indirectly via FSB-Avoidance. Findings suggest that more frequent use of avoidance behaviors to manage anxiety may maintain smoking and may partially explain the high rates of smoking among those with elevated social anxiety. Thus, FSB may be a promising target in smoking cessation interventions, especially among those with elevated social anxiety.
Kidney Cells Regeneration: Dedifferentiation of Tubular Epithelium, Resident Stem Cells and Possible Niches for Renal Progenitors

A kidney is an organ with relatively low basal cellular regenerative potential. However, renal cells have a pronounced ability to proliferate after injury, which undermines that the kidney cells are able to regenerate under induced conditions. The majority of studies explain yielded regeneration either by the dedifferentiation of the mature tubular epithelium or by the presence of a resident pool of progenitor cells in the kidney tissue. Whether cells responsible for the regeneration of the kidney initially have progenitor properties or if they obtain a “progenitor phenotype” during dedifferentiation after an injury, still stays the open question. The major stumbling block in resolving the issue is the lack of specific methods for distinguishing between dedifferentiated cells and resident progenitor cells. Transgenic animals, single-cell transcriptomics, and other recent approaches could be powerful tools to solve this problem. This review examines the main mechanisms of kidney regeneration: dedifferentiation of epithelial cells and activation of progenitor cells with special attention to potential niches of kidney progenitor cells. We attempted to give a detailed description of the most controversial topics in this field and ways to resolve these issues.
Neurocognitive impairment in Spanish-speaking Latinos living with HIV in the US: Application of the neuropsychological norms for the US–Mexico border region in Spanish (NP-NUMBRS)

Abstract Objective Latinos in the US are at increased risk for HIV-associated neurocognitive impairment (NCI). Most studies of US Latinos living with HIV have included primarily English-speakers only. We investigated the rate, pattern, and correlates of HIV-associated NCI in native Spanish-speaking Latinos living in the US near the Mexican border. Methods Participants included 407 native Spanish-speaking Latinos (Age: M = 37.65, SD = 10.0; Education: M = 10.75, SD = 4.1; 53% male): 153 persons living with HIV (PLWH; 56% AIDS) and 254 healthy controls. All participants completed comprehensive neuropsychological assessments in Spanish. Raw neuropsychological test scores from seven domains were converted to demographically-adjusted T-scores using norms developed with healthy controls. Global and domain NCI were defined per established criteria. Among PLWH we applied norms developed for non-Hispanic (NH) Whites and Blacks, and investigated correlates of global NCI, including HIV disease characteristics and psychiatric comorbidities. Results Utilizing population specific norms, rates of global NCI were significantly higher among PLWH (39%) than healthy controls (17%), comparable to previously published rates. In contrast, rates of global NCI in the same group of PLWH were significantly different when NH White norms (63%, p < 0.0001) and NH Black norms were used (18%, p < 0.0001). Among PLWH without a history of lifetime substance use disorder, more years of antiretroviral exposure were significantly associated with decreased rates of global NCI. Conclusions Present findings lend support to the validity of newly developed norms for native Spanish-speakers living near the US-Mexico border, and underscore the importance of utilizing appropriate norms to accurately identify HIV-associated NCI.
Risk factors for suicide attempts among rural youth with a history of suicidal ideation

Abstract Youths in rural areas have approximately double the risk for suicide than do urban youth. This study analyzed data from 580 youth aged 10 to 17-years-old who endorsed lifetime suicidal ideation on intake at three large rural behavioral health centers. Results indicated that a five-factor model including older age, number of inpatient hospitalizations, gender (female), impaired legal functioning, and higher impulsivity accounted for the most variance in differentiation between ideators and attempters. These results advance our assessment of rural youth who may be at increased risk for a suicide attempt.