Miracle-dz/UniFAR-FTUs huggingface dataset:
Hamiltonian four-field model for nonlinear tokamak dynamics

The Hamiltonian four‐field model is a simplified description of nonlinear tokamak dynamics that allows for finite ion Larmor radius physics, as well as other effects related to compressibility and electron adiabaticity. Much simpler than some previous descriptions of the same physics, it still preserves essential features of the underlying exact dynamics. In particular, because it is a Hamiltonian dynamical system it conserves the appropriate Casimir invariants, as well as avoiding implicit, unphysical dissipation. Here the model is derived and interpreted, its Hamiltonian nature is demonstrated, and its constants of motion are extracted.
Genetic discontinuity, breeding‐system change and population history of Arabis alpina in the Italian Peninsula and adjacent Alps

Arabis alpina is a widespread plant of European arctic and alpine environments and belongs to the same family as Arabidopsis thaliana. It grows in all major mountain ranges within the Italian glacial refugia and populations were sampled over a 1300 km transect from Sicily to the Alps. Diversity was studied in nuclear and chloroplast genome markers, combining phylogeographical and population genetic approaches. Alpine populations had significantly lower levels of nuclear genetic variation compared to those in the Italian Peninsula, and this is associated with a pronounced change in within‐population inbreeding. Alpine populations were significantly inbred (FIS = 0.553), possibly reflecting a change to the self‐incompatibility system during leading edge colonization. The Italian Peninsula populations were approaching Hardy–Weinberg equilibrium (outbreeding, FIS = 0.076) and genetic variation was highly structured, consistent with independent local ‘refugia within refugia’ and the fragmentation of an established population by Quaternary climate oscillations. There is very little evidence of genetic exchange between the Alps and the Italian Peninsula main distribution ranges. The Alps functioned as a glacial sink for A. alpina, while the Italian Peninsula remains a distinct and separate long‐term refugium. Comparative analysis indicated that inbreeding populations probably recolonized the Alps twice: (i) during a recent postglacial colonization of the western Alps from a Maritime Alps refugium; and (ii) separately into the central Alps from a source outside the sampling range. The pronounced geographical structure and inbreeding discontinuities are significant for the future development of A. alpina as a model species.
Lack of Association of the PICALM rs3851179 Polymorphism With Parkinson's Disease in the Greek Population

ABSTRACT Parkinson's disease (PD) is a complex, heterogeneous neurodegenerative disorder, affecting approximately 1% of the population over 60 years of age. The molecular and cellular mechanisms underlying PD pathogenesis are still unknown. Clathrin-mediated endocytosis (CME) is a procedure closely related to the intracellular trafficking of multiple molecules in the cell, including proteins, lipids, and neurotransmitters. Recently, variations in the gene encoding the phosphatidylinositol binding clathrin assembly protein (PICALM) has been associated with Alzheimer's disease (AD), suggesting a possible role of CME in the pathogenesis of neurodegenerative diseases. In this study, we examined for the first time the potential role of the PICALM rs3851179 polymorphism in PD. We studied the PICALM rs3851179 polymorphism in 191 Greek patients with sporadic PD and 118 control subjects, using a PCR-RFLP method. Our results do not provide evidence that the PICALM rs3851179 polymorphism increase susceptibility of PD, in the Greek population.
An efficient and realistic simulation platform suitable for the analysis and design of time-modulated arrays

The paper shows a nonlinear/electromagnetic co-simulation tool for the rigorous analysis of time-modulated arrays in realistic application scenarios, e.g. in presence of digitally modulated carriers. The Harmonic Balance (HB) technique is used for the accurate description of the nonlinearities, and is combined to the full-wave description of the radiating array. The developed tool overcomes simplifying assumptions of previous approaches and its effectiveness is verified with numerical examples in both modulated and unmodulated carrier cases. The method is also applied to different switch modulation patterns, thus showing a high versatility suitable to cover a large plethora of applications.
Australasian ( ACIS ) 1-1-2010 Development of knowledge management support for the sleep disorder diagnosis proces

Sleep disorders are a major problem in today’s society with a lack of sleep being associated with lost productivity and accidents. There is a need to improve the sleep disorder diagnosis process and this paper describes the work in progress for the development of a patient data gathering system and decision support system. This system is designed to support a sleep physician’s diagnosis of sleep disorders. To facilitate acceptance by physicians a participatory research approach to development was taken, involving physicians and a psychologist. This approach has produced a system which integrates into a physician’s work flow.
Glyceryl‐S‐Acyl Carrier Protein as an Intermediate in the Biosynthesis of Tetronate Antibiotics

The biosynthetic pathway to the unusual tetronate ring of certain polyketide natural products, including the antibiotics abyssomicin and tetronomycin (TMN) and the antitumour compound chlorothricin (CHL), is presently unknown. The gene clusters governing chlorothricin and tetronomycin biosynthesis both contain a gene encoding an atypical member of the FkbH family of enzymes, which has previously been shown to synthesise glyceryl‐S‐acyl carrier protein (ACP) as the first step in production of unusual extender units for modular polyketide biosynthesis. We show here that purified recombinant FkbH‐like protein, Tmn16, from the TMN gene cluster catalyses the efficient transfer of a glyceryl moiety from D‐1,3‐bisphosphoglycerate (1,3‐BPG) to either of the dedicated ACPs, Tmn7a and ChlD2, to form glyceryl‐S‐ACP, which directly implicates this compound as an intermediate in tetronate biosynthesis as well. Neither Tmn16 nor Tmn7a produced glyceryl‐S‐ACP when incubated, respectively, with analogous ACP and FkbH‐like proteins from a known extender‐unit pathway; this indicates a highly selective channelling of glycolytic metabolites into tetronate biosynthesis.
Growth related changes in sugar determinants on the surface of C6 glioma cells in culture: A cytochemical lectin‐binding study

The cell surface sugar determinants (CSSD) were examined in C6 glioma cells in cultures at different conditions of growth by peroxidase conjugates of the lectins: peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Helix pomatia agglutinin (HPA), wheat germ agglutinin (WGA), lentil agglutinin (LCA), laburnum bork agglutinin (LABA), and lotus agglutinin (TPA). It was found that the cells bound more intensively WGA, LCA, and RCA compared to PNA, HPA; the weakest staining was provided by LABA and TPA. Binding intensity for PNA significantly increased after pretreatment of the cells with neuraminidase. This indicates that a part of the β‐D‐galactose residues on the surface membrane of C6 glioma cells is covered by sialic acid. The process of sialization was increased during the culturing of C6 glioma cells. Addition of cis‐DDP or dBcAMP to cultures growing in medium with 10% of CS increased the number of Gal residues which are not covered by sialic acid.
ANALYTICAL CALCULATION OF SHAFT CURRENTS IN THE MOTORS WITH HOMOGENEOUS YOKE

Hederić, Ž. , Barukčić, M. , Miklošević, K. , Analitički proračun osovinskih struja . , Energija, god. 58(2009), br. 1. , str. 56-75 Hederić, Ž. , Barukčić, M. , Miklošević, K. , Analitical Calkulation of Shaft Currents. , Energija, vol. 58(2009), No. 1, pp. 56-75 ANALITIČKI PRORAČUN OSOVINSKIH STRUJA KOD MOTORA S HOMOGENIM JARMOM ANALYTICAL CALCULATION OF SHAFT CURRENTS IN THE MOTORS WITH HOMOGENEOUS YOKE
An efficient indoor location system in WLAN based on Database Partition and Euclidean Distance-Weighted Pearson Correlation Coefficient

This paper proposes an indoor location system in WLAN based on fingerprint Database Partition and Euclidean Distance-Weighted Pearson Correlation Coefficient which use a new method of partitioning the fingerprint database is PWNN(Nearest Neighbors based on Pearson correlation coefficient and Distance-weighted). This system includes three stages: offline data collection and pretreatment; Online positioning; Fixed positioning. The first stage partitions the fingerprint database in accordance with the maximum signal strength AP (Access Point) to improve the speed of matching. The second stage uses Pearson correlation coefficient to match the signal fingerprint and select the probability of the collection points, then applies NN algorithm and weighted Euclidean distance to estimate the position. The actual system test proves that the fusion algorithm can effectively improve positioning accuracy and greatly shorten positioning time. Thus, it is an effective and valid indoor positioning method.
Detection of Staphylococcal Superantigenic Toxins by a CD69-Specific Cytofluorimetric Assay Measuring T-Cell Activation

ABSTRACT The presence of staphylococcal superantigenic toxins in the supernatants of liquid cultures was detected by an easy and rapid method assessing the activation of T lymphocytes by cytofluorimetric measurement of CD69 expression. Staphylococcus aureus cells were grown in Eagle’s minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. aureus strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, and exfoliative toxins A and B, induced CD69 expression in a significantly higher number of T cells than a cutoff of 2%. This CD69 assay might be used for initial detection of superantigens from S. aureus strains isolated in the context of staphylococcal toxemia or related chronic human diseases such as atopic dermatitis or Kawasaki syndrome.
ldentification of a Fish Protein Associated with a Kinase Activity and Related to the Rous Sarcoma Virus Transforming Protein 1

Cells of all vertebrates thus far investigated contain a cellular src gene, which appears tobe a highly conserved homolog of the Rous sarcoma virus oncogene src. Similar to the Rous sarcoma virus oncogene src, this endogenaus cellular src gene codes for a M, 60,000 phosphoprotein (pp6oc-src) which is associated with a kinase activity. This kinase specifically phosphorylates tyrosine residues of proteins. We have systematically looked for pp6oc.src in specific strains of certain teleostean fish, including the genera Xiphophorus and Girardinus. These strains are known to contain chromosomal oncogenes causing neoplastic transformation in certain mutants and hybrids. Since the pp60c-src found in other species has been shown to be antigenically related to the viral src gene product, a phosphoprotein with a molecular weight of 60,000 (pp6ov-src), we have used tumor-bearing rabbit serum which recognizes Rous sarcoma virus pp6asrc to detect such a related protein in fish. Tumor-bearing rabbit serum precipitated two proteins with molecular weights of 60,000 and 52,000. The M, 60,000 phosphoprotein was associated with a protein kinase activity that phosphorylates tyrosine residues of the immunoprecipitating immunoglobulin G heavy chain (M, 53,000). The antigenic relationship of this fish protein to viral pp6osrc was further documented by an immune competition assay. We conclude, therefore, that the fish M, 60,000 phosphoprotein is a pp6oc-src encoded for by a fish cellular src gene that corresponds to the viral src oncogene. The M, 52,000 phosphoprotein is probably a proteolytic degradation product of pp6oc-src. All animals of the various strains of the fish investigated in this study showed kinase activity that was reactive upon immunoprecipitation with tumor-bearing rabbit serum, indicating that the pp6oc.src protein is a normal constituent of the fish. Measurable quantitative differences were revealed between the various strains as weil as in different tissues. Whereas brain and melanoma tissue showed a high kinase activity when compared to skin, liver, spleen, and testes, muscle tissue displayed no significant activity.
Endothelial Cell Costimulation of T Cell Activation Through CD58-CD2 Interactions Involves Lipid Raft Aggregation1

Human endothelial cells (EC) costimulate CD4+ memory T cell activation through CD58-CD2 interactions. In this study we tested the hypothesis that EC activate distinct costimulatory pathways in T cells that target specific transcription factors. AP-1, composed of fos and jun proteins, is a critical effector of TCR signaling and binds several sites in the IL-2 promoter. EC augment c-fos promoter activity in T cells; however, deletion analysis reveals no transcription factor binding sites in the promoter uniquely responsive to EC costimulation. Overexpression of AP-1 proteins in T cells augments the activity of an AP-1-luciferase reporter gene equally in the absence or the presence of EC costimulation. Interestingly, EC stimulate a similar 2- to 3-fold up-regulation of AP-1, NF-AT, NF-κB, and NF-IL-2-luciferase reporters. CD2 mAbs completely block EC effects on all of these pathways, as well as costimulation of IL-2 secretion. We conclude that EC costimulation through CD2 does not trigger a single distinct costimulatory pathway in T cells, but rather, it amplifies several pathways downstream of the TCR. Indeed, we find that early EC costimulation acts “upstream” of the TCR by promoting lipid raft aggregation, thus amplifying TCR signaling. Soluble CD2 mAbs block EC-induced raft aggregation, whereas cross-linking CD2 promotes aggregation. These data are consistent with the critical role of CD2 in organizing the T cell-APC contact zone.
The Art of Application Performance Testing - Help for Programmers and Quality Assurance

This practical book provides a step-by-step approach to testing mission-critical applications for scalability and performance before they're deployed -- a vital topic to which other books devote one chapter, if that. Businesses today live and die by network applications and web services. Because of the increasing complexity of these programs, and the pressure to deploy them quickly, many professionals don't take the time to ensure that they'll perform well and scale effectively. The Art of Application Performance Testing explains the complete life cycle of the testing process, and demonstrates best practices to help you plan, gain approval for, coordinate, and conduct performance tests on your applications. With this book, you'll learn to: Set realistic performance testing goals Implement an effective application performance testing strategy Interpret performance test results Cope with different application technologies and architectures Use automated performance testing tools Test traditional local applications, web-based applications, and web services (SOAs) Recognize and resolves issues that are often overlooked in performance tests Written by a consultant with 30 years of experience in the IT industry and over 12 years experience with performance testing, this easy-to-read book is illustrated with real-world examples and packed with practical advice. The Art of Application Performance Testing thoroughly explains the pitfalls of an inadequate testing strategy and offers you a robust, structured approach for ensuring that your applications perform well and scale effectively when the need arises. "Ian has maintained a vendor-agnostic methodology beautifully in this material. The metrics and graphs, along with background information provided in his case studies, eloquently convey to the reader, 'Methodology above all, tools at your discretion. ' Ian's expertise shines through throughout the entire reading experience. " -- Matt St. Onge, Enterprise Solution Architect, HCL Technologies America / Teradyne
Pressure-assisted cell spinning: a direct protocol for spinning biologically viable cell-bearing fibres and scaffolds

We recently pioneered the ability to directly electrospin living cells from which scaffolds to membranes were derived. This protocol, now widely referred to as ‘cell electrospinning’, is currently undergoing in-depth investigations where the post-treated cell's global gene expression to its sub-cellular components is being investigated for understanding any effects post-treating. Our motivation is to develop this method for the biomedical sciences, in particular for applications in regenerative and therapeutic medicine. In the current work, we unveil a direct cell spinning protocol which is non-electric field driven and which will compete directly with cell electrospinning. We referred to this processing method as ‘pressure-assisted spinning’ in our previous studies, where we demonstrated this route as an emerging micro/nanotechnology. In the current context, we refer to this processing protocol as ‘pressure-assisted cell spinning’ (PACS). Our developmental studies on PACS reported here show, for the first time, that this technique could be explored as an alternative approach to cell electrospinning. Pressure-assisted cell spinning now enters the direct biological scaffold to membrane formation league.
Intra-temporal facial nerve centerline segmentation for navigated temporal bone surgery

Approaches through the temporal bone require surgeons to drill away bone to expose a target skull base lesion while evading vital structures contained within it, such as the sigmoid sinus, jugular bulb, and facial nerve. We hypothesize that an augmented neuronavigation system that continuously calculates the distance to these structures and warns if the surgeon drills too close, will aid in making safe surgical approaches. Contemporary image guidance systems are lacking an automated method to segment the inhomogeneous and complexly curved facial nerve. Therefore, we developed a segmentation method to delineate the intra-temporal facial nerve centerline from clinically available temporal bone CT images semi-automatically. Our method requires the user to provide the start- and end-point of the facial nerve in a patient's CT scan, after which it iteratively matches an active appearance model based on the shape and texture of forty facial nerves. Its performance was evaluated on 20 patients by comparison to our gold standard: manually segmented facial nerve centerlines. Our segmentation method delineates facial nerve centerlines with a maximum error along its whole trajectory of 0.40±0.20 mm (mean±standard deviation). These results demonstrate that our model-based segmentation method can robustly segment facial nerve centerlines. Next, we can investigate whether integration of this automated facial nerve delineation with a distance calculating neuronavigation interface results in a system that can adequately warn surgeons during temporal bone drilling, and effectively diminishes risks of iatrogenic facial nerve palsy.
Occurrence of Multiorgan Dysfunction in Pediatric Burn Patients: Incidence and Clinical Outcome

Objective:To examine the incidence of single or multiple organ failure postburn and its resultant clinical outcomes during acute hospitalization. Background:Patient outcomes are inherently dependent on intact organ function; however, burn injury affects the structure and function of almost every organ, but especially lung, liver, kidney, and heart. Therefore, single-organ failure and/or multiorgan failure (MOF) are thought to contribute significantly to postburn morbidity and mortality, but to date no large trial examining the effects of MOF on postburn outcomes exists. Methods:Incidence of MOF was monitored in 821 pediatric burn patients during acute hospitalization. Patients were divided into groups on the basis of the incidence of single-organ–specific failure, MOF, and non-MOF. The DENVER2 score was used to assess organ-specific scores for lung, liver, kidney, and heart. The patient's demographics, injury characteristics, and outcome parameters were recorded. Results:Respiratory failure has the highest incidence in the early phase of postburn injury and decreases starting 5 days postburn. Cardiac failure was noted to have the highest incidence throughout hospital stay. Incidence of hepatic failure increases with the hospital length of stay and is associated with a high mortality during the late phase of the acute hospital stay. Renal failure has an unexpectedly low incidence but is associated with a high mortality during the first 3 weeks postburn injury. Three or more organ failure is associated with very high mortality. Conclusions:This is the first large study in burn patients to determine the incidence of organ-specific failure and outcome. The results of this study confirmed the expected chronologic incidence of organ-specific failure and yield the long-term mortality from liver and renal failure (NCT00673309).
Initiation of Genetic Demographic Monitoring of Bonobos ( Pan paniscus ) at Iyema , Lomako Forest , DRC

Research on wild apes is not only fundamental for elucidating human origins but for their conservation as well. Despite their relative size, apes are difficult to observe in the wild prior to habituation, limiting our ability to accurately assess demography and kin relations. Non-invasive genetic sampling provides an indirect source of this information. Here, we present findings of a pilot genetic survey of a wild community of bonobos that are in the initial stages of being studied. Fifty-three fecal samples were collected over eight days near the Iyema field site in the Lomako Forest, DRC. DNA was first extracted and quantified using a qPCR assay. Samples with a sufficient amount of DNA were genotyped at 11 microsatellite loci and sexed using an amelogenin assay. Thirty-three of 53 samples yielded a sufficient amount of DNA for complete genotyping. We identified 19 individuals, including six males and 13 females. Mean allelic richness across all loci was 5.7 and expected heterozygosity was 0.69. Estimates of population size indicate between 26 and 66 individuals are present in the study area, but more than one community may be present. These results contribute to our ongoing efforts to study and monitor the bonobos at Iyema to better understand their demography, behavior, and conservation. Our study also highlights the utility of genetic analyses in pilot and survey research.
Review authenticity verification using supervised learning and reviewer personality traits

Social media has increasingly promoted users to provide customer feedback on shopping experiences in the form of online product reviews. Many major ecommerce sites are collecting revenue from advertisements of products and creating better shopping experiences for customers with the help of such reviews. The problem of accurately verifying review authenticity steadily grows and can help in making or breaking the good name of a product. Feature engineering performed on the reviews assist in extracting the useful features that help in identifying actual fake reviews. Supervised machine learning algorithms help in classifying reviews into authentic and fake. The newly emerging phenomenon of personality prediction has taken hold of social media and this is being employed in the case of reviewer traits which will help identify key personality traits of fake reviewers. The Big 5 model is used for this which will be useful in tracking such people through their associated social media accounts.