Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical Utility of GST and CEA in Gastrointestinal Cancer

: Purpose: - To analyze the level of serum Glutathione-S-Transferase (GST) and Carcinoembryonic antigen (CEA) before and after different Cycles Of chemotherapy in GI carcinoma patients. Methods: - For the study comprising total 58 cases suffering from GI carcinoma stage I, stage II stage III and Stage IV (before and after different cycle of chemotherapy) were selected. All patients were clinically and histologically diagnosed. A total of 42 age and sex matched healthy subjects taken as control. The circulating levels of GST and CEA activity were assayed in the in the serum of control group and in patients with GI carcinoma. Results: - Serum level of GST and CEA were highly significant in GI carcinoma patients as compared to control group (p<0.001). After first cycle of chemotherapy (stage II) the activity of GST and CEA were significantly higher than before chemotherapy (stage I). In stage III (after second cycle of chemotherapy) activity was significantly decreased than that of stage II and the activity of GST and CEA was significantly decreased in stage IV (after third cycle of chemotherapy) than stage III (after second cycle of chemotherapy). Conclusion: - On the basis of data from our study, it can be stated that serum GST measurement in plasma may be useful tumor marker in gastrointestinal carcinoma, its activity might helpful to predict the response of chemotherapy in advance stage of cancer. CEA is a tumor marker that is measured using a blood test.CEA tumor marker is one of the general type tumor markers. A multiply increased CEA levels in the blood indicate to the presence of a malignant disease in the body, but not to the organ in which the malignant change has occurred. High levels of CEA may indicate that cancer has spread; however, other medical conditions and some treatments, including certain types of chemotherapy, may raise CEA levels. CEA tests are one way doctors can find out whether the cancer has spread or returned. Cancer that has spread or returned can be treated successfully for many patients. CEA measurement is mainly used to identify recurrences after surgical resection and for staging.
A Combination of GA and PSO for Automatic Test Data Generation using Data Flow Coverage

: . Software testing plays an important role for software’s quality and reducing the cost. In this paper we introduce a new algorithm that combine the power of Genetic Algorithm (GA) and Particle Swarm Optimization (PSO) called Genetic-Particle Swarm Combined Algorithm (GPSCA) which is used to generate automatic test data that satisfy data-flow coverage criteria. Finally, the paper presents the results of the experiments that have been carried out to evaluate the effectiveness of the proposed GPSCA with new fitness function compared to the Genetic Algorithm and PSO algorithms.
Amino Acid Residues Required for Maturation, Cell Uptake, and Processing of Translation Inhibitor Microcin C (cid:1)

Microcin C (McC), a peptide-nucleotide Trojan horse antibiotic, targets aspartyl-tRNA synthetase. We present the results of a systematic mutational study of the 7-amino-acid ribosomally synthesized peptide moiety of McC. Our results deﬁne amino acid positions important for McC maturation and cell uptake and processing and open the way for creation of more potent McC-based inhibitors. The microcins are a class of small ( (cid:2) 10 kDa) antibiotics produced from ribosomally synthesized peptide precursors (1). Genes responsible microcin production
A Rice Brassinosteroid-Deficient Mutant , ebisu dwarf ( d 2 ) , Is Caused by a Loss of Function of a New Member of Cytochrome P 450

Shigeo Yoshida, c Motoyuki Ashikari, a Hidemi Kitano, a and Makoto Matsuoka a,1 a BioScience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan b Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan c RIKEN (The Institute of Physical and Chemical Research), Wako-shi, Saitama 351-0198, Japan d Department of Chemistry, Joetsu University of Education, Joetsu-shi, Niigata 943-8512, Japan
T HE C ONTRIBUTION OF M AXIMAL F ORCE P RODUCTION TO E XPLOSIVE M OVEMENT A MONG Y OUNG C OLLEGIATE A THLETES

B.A. Alvar, and M.R. Rhea. The con- tribution of maximal force production to explosive movement among young collegiate athletes. J. Strength Cond. Res. 20(4): 867–873. 2006. —Critical to multidimensional sport conditioning is a systematic knowledge of the interactions between ﬁtness components, as well as the transference relationships to performance. The purpose of this investigation was to examine the relationships between lower body muscular strength and several fundamental explosive performance measures. Fifty-four men and women collegiate athletes were tested to determine (a) low-er-body muscular strength (1 repetition maximum barbell back squat), (b) countermovement vertical jump height and peak power output, (c) standing broad jump distance, (d) agility (cone T- test time), (e) sprint acceleration (m·s (cid:2) 2 ), and (f) sprint velocity (m·s (cid:2) 1 ). Analyses were performed using Pearson r correlations to examine these relationships. Partial correlations tested for relationships between performance measures while controlling for muscular strength. T-tests were performed to assess the dif-ference between men and women. Correlation data demonstrat- ed that signiﬁcant ( p (cid:3) 0.01) strong linear relationships were indicated between muscular strength and power, as well as ev- ery sport-performance ﬁeld tests. However, when controlling for strength with partial correlation, each of these relationships ap- preciably diminished. Signiﬁcant differences ( p (cid:3) 0.05) were found between men and women for each of the performance tests. Muscular strength, peak power output, vertical jumping ability, standing broad jump, agility, sprint acceleration, and sprint velocity were all shown to be very highly related. Further examination demonstrated that body mass–adjusted muscular strength is more highly related to performance measures than is absolute muscular strength. Current correlation data provide a quantiﬁed look at the interaction between muscular ﬁtness components, as well as the transfer relationship to several ath- letic-speciﬁc performance measures.
OTT_A_265591 683..695

Department of Orthopedics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 261400, People’s Republic of China Purpose: There is increasing evidence that non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), produce a critical regulatory effect on osteosarcoma (OS). LINC01278, as a newly discovered lncRNA, is found to be highly expressed in OS, but its related mechanism remains unclear. This research, therefore, is designed to study the mechanism of LINC01278 in OS and to find potential targets for clinical use. Methods: qRT-PCR was applied to determine the relative expression of LINC01278 and analyze its diagnostic value in OS. CCK-8, Transwell and flow cytometry were utilized for the determination of cell proliferation, migration/invasion, and apoptosis. RIP and RNA pulldown experiments were used to verify the targeted binding effect of miR-134-5p and LINC01278. The relationship between miR-134-5p and LINC01278 or KRAS was analyzed using dual luciferase reporter gene. The effects of LINC01278 on tumor growth in nude mice was analyzed by in vivo experiment. Results: qRT-PCR showed that LINC01278 increased in OS tissues and serum, indicating poor prognosis. In addition, LINC01278 was also of high value for OS diagnosis. Functional experiments showed that LINC01278 inhibited KRAS-mediated OS cell proliferation and metastasis through miR-134-5p. Finally, the results of an in vivo animal model indicated that LINC01278 promoted OS growth. Conclusion: LINC01278 is expressed highly in OS, and patients with high LINC01278 expression have poor prognosis. Moreover, LINC01278 can suppress the proliferation and apoptosis of OS cells through mediating miR-134-5p/KRAS axis, which is expected to become a potential therapeutic target for OS.
An Automated Scheme For Surveillance Videos Footage And The System Searches

The research presented an automated scheme for surveillance videos footage and the system searches Person of Interest (POI) and retrieves video shots for specific POI. An automated system has four stages; 1) to detect shot boundaries and divides original video into shots is called shot detection stage, 2) detection and recognition of face stage that detect faces in video footage using based on Haar-linke features and using the Principal Component Analysis algorithm (PCA) for extraction of features in order to compute and select the eigenvectors with the major eigenvalues, 3) groups and apply face annotation with similar facial features into the same cluster, and 4) retrieve specifics person footage from database user generate query by person image. The proposed scheme is user friendly and simple. It really overcome workload and enhances accuracy to localized person of interest in surveillance videos data. The average recognition accuracy on NRC-IIT [1] face video data set is 83.2 percent.
UNIVERSITY OF CALIFORNIA, MERCED Mechanical Signaling and Patterning Stem Cell-derived Vascular Cells A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Materials and Biomaterials Science Engineering by

In addition to providing cells sourcing for many cell therapies, ESC and induced pluripotent stem (iPS) cells are excellent cell culture systems for elucidating developmental signals. ESC and iPS cells are traditionally cultured and induced into tissue-specific derivatives on tissue culture plastic, however; it has been recently shown that the stiffness of the substrate also plays a role in directing cells towards differential cell lineages. Using our chemically-defined and staged induction methods, the putative role for stiffness in vascular fate was examined in 2 distinct ESC lines. After initial induction and purification of multipotent Flk-1+ vascular progenitor cells (VPC), the diverging endothelial cell (EC) and smooth muscle cell (SMC) populations were quantified for expression of either platelet endothelial cell adhesion molecule (PECAM1). Here, we were able to culture mouse ESC VPC on single stiffness polyacrylamide hydrogels and observed EC and SMC emerging in a spatially distinct arrangement and identify signaling pathways that may be contributing to stiffness directed vascular differentiation.
Bringing outsourced functions back home

Introduction: Outsourcing as a phenomenon has recently gained a lot of attention and is by some even considered as a trend. Traditionally, the functions to outsource have often been activities like computer operations or production with the purpose to cut costs or utilize on resources not available in-house. The issues of outsourcing are however increasing, making the insight of the sources of competitive advantage and core competences more important than ever. With the rising amount of outsourced functions in firms of today, more and more outsourcing projects tend to end up with poor results. The combination of suppliers failing to live up to the standards set and an increased pressure of cost savings and quality improvements from customers is becoming untenable for many companies. As a result, large efforts are put into coordination and training and some companies even break their outsourcing agreement to either turn to another supplier or bring the function back in-house. Motives for bringing an outsourced function back in-house are often concerned with issues of quality and control. Purpose: The purpose is to determine whether backsourcing is merely a tool to correct implications of unsuccessful outsourcing agreements or if there are other underlying motives to the process. Method: The study has been conducted according to the qualitative research approach. Empirical data has been collected through personal semi-structured interviews with representatives from three different companies that recently have backsourced a business function. The empirical data have been analyzed in the light of relevant theories concerning outsourcing and backsourcing. Analysis: The analysis indicates on both differences and similarities of motives for, and implications due to outsourcing among the investigated companies. The same is evident for the backsourcing of the specific functions. Conclusions: From the analyzed data, conclusions can be drawn that all the companies suffered from implications from their respective outsourcing agreements and used backsourcing to compensate for these. However, the process has, in some cases, increased knowledge and made the function perform better once taken back in-house.
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Reveals Broad Alterations in Hepatic Lipid Composition in an Experimental Mouse Model of Nonalcoholic Fatty Liver Disease

Non-Alcoholic Fatty Liver Disease (NAFLD) is among the commonest causes of liver disease in the United States. Its progression to Non-Alcohol Steatohepatitis (NASH) increases risk for developing cirrhosis and liver cancer. Hepatocellular accumulation of triglycerides and cholesterol, the main lipids associated with NAFLD, is considered benign. In contrast, aberrant expression of sphingolipids and phospholipids that have structural and functional roles in cell membrane integrity and intra-cellular signaling, may mediate progression of NAFLD to NASH. This study utilized an established experimental model of NAFLD gen-erated after 16 weeks of High Fat Diet (HFD) feeding of adult (8 weeks old) male C57BL/6 mice. Fresh frozen liver tissue samples were used for lipidomics analysis by matrix-assisted laser desorption ioniza-tion-imaging mass spectrometry in the negative and positive ion modes. In HFD fed mice, histopatholog-ical changes of NAFLD were associated with pronounced alterations in hepatic lipid profiles marked by increased expression of phosphatidylcholines (54%), phosphatidylinositols (50%), phosphatidylglycerols (50%), and phosphatidylinositol monomannosides (100%); sphingolipids including ceramides (63%), sphingomyelins (54%), sulfatides (57%), mannose inositol phosphoceramides (100%), and glycosphin-golipids (50%); and glycerolipids including triacylglycerols (56%). In addition, NAFLD was associated with increased levels of hepatic arachidonic acid containing phosphatidylserine, phosphatidylinositol, and phosphatidylethanolamine species and depletion of docosahexaenoic acid containing phosphatidylserine. Correspondingly, PCA plots sharply distinguished between the HFD and low-fat diet control groups. Ex- perimental NAFLD is associated with a broad array of increased hepatic lipids expression. These results establish a platform for evaluating mechanisms and consequences of hepatic lipidomic abnormalities that occur with progression from of NAFLD to NASH.
Stellate Ganglion Block Brain Protection During Ischemic Stroke in Diabetes Rats: TLR4/NF-κB Pathway and In ammation

Ting-ting Li Second A liated Hospital of Harbin Medical University Qiang Wan Second A liated Hospital of Harbin Medical University Xin Zhang Second A liated Hospital of Harbin Medical University Yuan Xiao Second A liated Hospital of Harbin Medical University Xiang-nan Liu Second A liated Hospital of Harbin Medical University Wan-chao Yang (  dachao_1980@126.com ) Second A liated Hospital of Harbin Medical University https://orcid.org/0000-0001-9964-9528
ARY ification of a germ-cell-specific transcriptional r tex-1

Develop one of t tebrates and germ genesis descript fertility molecul expression between the different stages of spermatocyte development and between wild-type and mutant mice has been exploited to identify regulatory elements necessary for differentiation and proliferation of the germ line. One set of mutants frequently used in studies of spermatogenesis are t-haplotypes. These are variant forms of mouse chromosome 17 which exist at a frequency of about 25% in wild Mus musculus populations (Bennett, 1975). t-haplotypes are distinguished from wild-type chromosomes by a multiplely inverted region of chromatin spanning about 15 cM known as the t complex (Shin et al., 1984; Silver, 1985). Recombinational suppression resulting from these inversions has allowed for the accumulation of recessive embryonic lethal mutations comprising sixteen different complementation groups. t-haplotypes, which carry one or more of these lethal mutations, have overcome the strong negative selection associated with lethality and been maintained in wild populations by transmission ratio distortion (TRD), i.e. meiotic drive (Chesley and n th rred igh 197 type ion ctio istor r loc cula sion has not been determined (Lyon, 1984; Silver and Remis, 1987). It is known that during spermiogenesis spermatids carrying t-haplotypes somehow render their meiotic partner virtually incapable of fertilizing the ovum (Silver and OldsClarke, 1984; Seitz and Bennett, 1985). Males heterozygous for the t complex produce, along with perfectly normal sperm, an abnormally high proportion of gametes that prematurely release their acrosome contents and abnormally high proportions with defective motility (Olds-Clarke, 1983; Brown et al., 1989). It is not known, however, whether these defective sperm are the ‘poisoned’ wild-type meiotic products. While the underlying cause of these phenomena remains a mystery, the genes involved are likely to play fundamental roles in spermatogenesis. Several candidate genes mapping to the loci mentioned above have been cloned by ourselves and others using a variety of molecular techniques. (Schimenti et al., 1988; Lader et al., 1989; Ha et al., 1991). We previously identified two candidates (Tctex-1 and TctexDevelopme Printed in G
Factors in ﬂ uencing insulin sensitivity during hyperinsulinemic-euglycemic clamp in healthy Korean male subjects Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy

Background and objective: The effects of age and related factors on insulin sensitivity have not been de ﬁ nitively evaluated in East Asian populations. We proposed a reference range for the glucose disposal rate (M-value) on hyperinsulinemic-euglycemic study and its association with other parameters. Methods: Healthy, non-diabetic young (n=10) and elderly (n=13) male subjects with normal body mass index were eligible for this study. Subjects who passed the oral glucose tolerance test (OGTT) underwent hyperinsulinemic-euglycemic clamp with high-dose (80 mU/m 2 ·min) insulin infusion. Results: M-values were normalized to body weight (M BW ) and fat-free mass (M FFM ). Neither M-value was signi ﬁ cantly different between age groups ( P =0.458 and P =0.900, respectively). An inverse correlation was observed between M FFM and baseline insulin ( r = − 0.418; P =0.047), baseline C-peptide ( r = − 0.426; P =0.043) and OGTT 2-hour glucose ( r = − 0.452; P =0.030). Regarding correlations with other insulin sensitivity indices, M-values were positively associated with the Matsuda index but not with homeostasis model assessment of insulin resistance. Conclusion: Our results suggest that age is not a critical determinant of insulin sensitivity, while fasting insulin and C-peptide levels, OGTT 2-hour glucose level, and Matsuda index are predictable markers of insulin sensitivity in healthy Koreans. transaminase; CV, coef ﬁ cient of variation; GGT, gamma-glutayltransferase; HbA1c, hemoglobin A1c; HDL, high-density lipoprotein; HOMA-IR, homeostasis model assessment of insulin resistance; LDL, low-density lipoprotein; FFM, fat-free mass; OGTT, oral glucose tolerance test; TG, triglyceride.
OCCUERRENCE OF THERMOTOLERANT CAMPYLOBACTER S IN TABLE EGGS

Thermotolerant Campylobacter spp. particularly C. jejuni and C. coli are recognized as the leading causes of bacterial foodborne diarrheal disease throughout the developed and developing world for more than century. A total of 500 eggs of Baladi and large scale eggs (250 eggs each) were collected from farmers' houses and markets in Qena Governorate. Samples were divided into pools (each pool contained 5 eggs obtaining 100 pools) and were examined for existence of thermotolerant Campylobacter spp. on their egg shells and in their inner content. The biochemically identified C. jejuni and C. coli isolates were confirmed using multiplex PCR. Thermotolerant Campylobacter spp. were isolated from 15 and 5% of egg shell and egg content samples, respectively. Isolates from egg shell samples were distributed as C. jejuni (9%), C. coli (4%), C. lari (1%) and C. upsaliensis (1%), while isolates from egg content samples were identified as C. jejuni (4%) and C. lari (1%). Large scale egg samples which are produced by poultry farms lacked thermotolerant Campylobacter spp. Whereas, 30 and 10% of Baladi egg samples collected from farmers' houses were contaminated with thermotolerant Campylobacter spp. on their egg shell and in their content, respectively. This study focuses on the risk of transmission of thermotolerant Campylobacter spp. through table eggs, especially that produced by farmers' houses (Baladi egg), in Qena.
Distributed Resilient Control Plane for Large Software Defined Networks

—Large Software Defined Networks (SDN) solve the control scalability problem coming from the SDN control centralization principle, by defining and installing several regional controllers. Therefore, a controller placement problem (CPP) should be solved. During run-time, possible failures of links or node appear; then a forwarder node could try to select an available and reachable controller among those which are functional. This is called controller selection problem (CSP). Although many studies have been published, the above problems are still open research issues, given the various network contexts, providers’ policies and possible multiple, different optimization criteria. Therefore, multi-criteria decision algorithms can provide valuable solutions. This paper is based on a previous work which has developed a simulation model for multi-controller SDN network, targeting optimization while including resilience aspects of the controller placement problem and controller selection problem. The current paper extends that work, by including a more in-depth analysis, giving relevant examples and introducing additional novel experiment results.
p27 1 Is Required for PTEN-induced G 1 Growth Arrest

The tumor suppressor PTEN is one of the most commonly inactivated genes in human cancer. Glioblastoma multiforme cells harboring mutant PTEN have abnormally high levels of 3 * phosphoinositides and elevated protein kinase B activity. Expression of wild-type PTEN in glioma cells, containing endogenous mutant PTEN, reduces 3 * phosphoinositides levels, inhibits PKB activity, and induces G 1 cell cycle arrest. We investigated the mechanism of the PTEN-induced growth arrest in glioma cell lines. Expression of PTEN is associated with increased expression of p27 Kip1 , decreased expression of cyclins A and D3, inhibition of cdk2 activity, and dephosphor- ylation of pRb. Inactivation of p53, by the human papilloma virus E6 oncoprotein, does not prevent PTEN-induced G 1 arrest, implying that p53 is not required for G 1 arrest. In contrast, p27 Kip1 antisense oligonucleotides abro- gated the growth arrest induced by PTEN. Furthermore, blocking p27 Kip1 expression prevented the PTEN-induced reduction of cyclin-dependent ki- nase 2 activity, indicating that p27 Kip1 functions upstream of cyclin-depend-ent kinase 2 in the PTEN regulatory cascade. These results implicate p27 Kip1 as a critical mediator of PTEN-induced G 1 arrest.