Miracle-dz/UniFAR-FTUs huggingface dataset:
Ocean acidification and warming affect skeletal mineralization in a marine fish

Ocean acidification and warming are known to alter, and in many cases decrease, calcification rates of shell and reef building marine invertebrates. However, to date, there are no datasets on the combined effect of ocean pH and temperature on skeletal mineralization of marine vertebrates, such as fishes. Here, the embryos of an oviparous marine fish, the little skate (Leucoraja erinacea), were developmentally acclimatized to current and increased temperature and CO2 conditions as expected by the year 2100 (15 and 20°C, approx. 400 and 1100 µatm, respectively), in a fully crossed experimental design. Using micro-computed tomography, hydroxyapatite density was estimated in the mineralized portion of the cartilage in jaws, crura, vertebrae, denticles and pectoral fins of juvenile skates. Mineralization increased as a consequence of high CO2 in the cartilage of crura and jaws, while temperature decreased mineralization in the pectoral fins. Mineralization affects stiffness and strength of skeletal elements linearly, with implications for feeding and locomotion performance and efficiency. This study is, to my knowledge, the first to quantify a significant change in mineralization in the skeleton of a fish and shows that changes in temperature and pH of the oceans have complex effects on fish skeletal morphology.
Electroporation of Ishikawa cells: analysis by flow cytometry.

Electroporation facilitates loading of cells with molecules and substances that are normally membrane impermeable. Flow cytometry is used in this study to examine the effects of the application of electroporation-level monopolar electric field pulses of varying electrical field strength on Ishikawa endometrial adenocarcinoma cells. Analysis of the fluorescence versus forward scatter plots corroborates the well-recognised threshold and cell size dependence characteristics of electroporation, but also shows the progression of cell lysis and generation of particulate material. Two 500 µs monopolar rectangular pulses ranging from 1.0 × 105 to 2.5 × 105 V/m were used to electroporate the cells. Electroporation yields (fraction of viable cells exhibiting significant propidium iodide uptake) ranged from 0 to 97%, with viability ranging between 78 and 34% over the electric field strength range tested. The higher electric field strength pulses not only reduced cell viability, but also generated a substantial amount of sub-cellular sized particulate material indicating cells have been physically disrupted enough to create these particles.
A novel JK null allele in a Brazilian patient with sickle cell disease (SCD)

T he Kidd antigens are clinically relevant blood group antigens, notorious for causing hemolytic transfusion reactions. The Kidd glycoprotein is encoded by the SLC14A1 gene with two major codominant alleles, JK*A (JK*01) and JK*B (JK*02), which result from a single-nucleotide polymorphism, 838A>G. JK null phenotypes have primarily been found in the Polynesian, Asian, and Finnish populations, but other null and variant alleles have also been identified in people of other ethnic backgrounds. Here, we report a novel JK null allele found in a 37-year-old African Brazilian patient with sickle cell disease (SCD) without recent transfusions admitted in the hospital with hemoglobin of 6.4 g/dL who was predicted to type Jk(a+b+) by a genotyping panel (ID-CORE XT, Progenika, Grifols), but who typed Jk(a–b+) with BioRad antisera in gel cards.
Ultrasound of entheses in ankylosing spondylitis patients: The importance of the calcaneal and quadriceps entheses for differentiating patients from healthy individuals

OBJECTIVES: To compare the ultrasonographic findings of entheses in ankylosing spondylitis (AS) patients with those of healthy control individuals and to assess the ability of ultrasound (US) to identify aspects related to the disease. METHODS: A cross-sectional study involving 50 patients with AS and 30 healthy controls was performed. Clinical assessment included the use of a visual analog scale for pain, assessment of swelling of the enthesis, global assessments for patients and physician, use of a disease activity index, mobility and dysfunctional indices, erythrocyte sedimentation rate and clinical enthesitis index. US was performed for the following entheses by two experienced musculoskeletal radiologists: brachial triceps, distal quadriceps, proximal and distal patellar tendons, calcaneal tendon, and plantar fascia; the total and subitems of the Madrid Sonographic Enthesitis Index were used for evaluations. RESULTS: Comparison between groups showed a statistically significant difference with worse scores in AS patients, with bone erosion of the calcaneal enthesis and bone erosion and thickening of the plantar fascia. The odds ratio for thickening of the plantar fascia in the AS group was 3.47, according to logistic regression analysis. The AS group also had worse scores regarding the presence of calcification in the quadriceps enthesis, with a fivefold increased risk. CONCLUSION: US analysis showed that only entheses of the foot and quadriceps were able to differentiate AS patients from healthy individuals.
Surface Probing by Spectroscopy on Titania-Supported Gold Nanoparticles for a Photoreductive Application

The continuous increase in scientific reports concerning photocatalysis and in particular CO2 photoreduction in recent years reveals the high degree of interest around the topic. However, the adsorption and activation mechanisms of CO2 on TiO2, the most used photocatalyst, are poorly understood and investigated. Gold nanoparticles were prepared by a modified deposition-precipitation method using urea and a chemical reductant. Bare P25 was used as reference. Combined spectroscopic investigations of fresh and spent samples with photoactivity studies reported in this article provide new insights to the role of CO2 adsorption and carbonate formation on Au/TiO2 during CO2 photocatalytic reduction. The key intermediates’ and products’ adsorption (CO, methanol, ethanol) was studied, coupled with X-ray photoelectron microscopy (XPS) and UV-Visible spectroscopy. The adsorption of CO2 on fresh and spent catalysts changes radically considering the carbonate formation and the gold surface presence. Methanol and ethanol revealed new adsorbed species on Au with respect to bare titania. The characterisation of the spent catalysts revealed the good stability of these samples.
Adsorption separation of CO from syngas with CuCl@AC adsorbent by a VPSA process

In this work, activated carbon (AC) supported CuCl (CuCl@AC) prepared with CuCl2 as precursor is investigated for CO separation from the synthesis gas mixture. Firstly, the CuCl@AC adsorbents are investigated for their CO reversible adsorption capacity at an operation temperature of 303 K. And a vacuum pressure swing adsorption (VPSA) process of CO separation from syngas utilizing the prepared CuCl@AC adsorbent is investigated at ambient temperature and 0.79 MPa through a dynamic optimization with Aspen Adsorption software. The integrated model is closer to a realistic PSA process, making the results of the simulation and optimization more convincing. The adsorption result reveals that the obtained CuCl@AC adsorbent with the copper loading of 7 mmol g−1 AC achieves a high reversible CO adsorption capacity and adsorption selectivity. The simulation result shows that, under optimal conditions, the CO product with the purity of 98.1 vol% can be separated from the syngas with the CO concentration of 32.3 vol% utilizing the prepared CuCl@AC adsorbent, and the recovery of CO is 92.9%.
Basal and Foliar Treatment using an Organic Fertilizer Amendment Lowers Cadmium Availability in Soil and Cadmium Uptake by Rice on Field Micro-Plot Experiment Planted in Contaminated Acidic Paddy Soil

ABSTRACT In the present study, field micro-plot experiments were conducted to investigate the basal and foliar application of a tested organic fertilizer amendment (OFA) for decreasing the risk of Cd accumulating in rice. The results showed that applications of OFA significantly increased rice yields in Cd-polluted soil and reduced the level of Cd in rice plants, especially in rice grain. In addition, three application methods of OFA were investigated (single basal application (B1, B2, and B3), combined basal application (+LM, +D, and +Z), and foliar application (F1, F2)). Treat B, F, +LM, +D were all higher than control on rice yield with 25.03, 28.05, 30.61, 22.50 g pot−1 on average, respectively. Among which, rice cadmium depress to 0.33 mg kg−1 in foliar application is considered to be a more efficient and economical method of heavy metal remediation. The mechanism of foliar application to alleviate the accumulation of Cd in brown rice may be related to the probable Cd sequestration in the leaves and straws. And the doses of the foliar application were 2.25–3.75 kg hm−2, approximately 1.0–2.5% of the basal application amount yet with more effect (0.10 mg kg−1 more than single basal; 0.23 mg kg−1 more than combined basal) on Cd reduction.
Series solution of a nonlocal dielectric test model for a solute ball with point charges in an ionic solvent

This paper reports an analytical solution of a nonlocal dielectric test model for a solute ball with multiple point charges in an ionic solvent. The solution is derived in a single sum series of Legendre polynomials and modiﬁed spherical Bessel functions so that it can be calculated eﬃciently for a large set of charges or a large set of mesh points. It also contains the series solution of a two-region Kirkwood ball model as a special case. This series solution can be valuable in the study of nonlocal dielectric continuum models and in the validation tests on the numerical algorithms and software packages for solving nonlocal Poisson-Boltzmann equation models.
Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening

This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii . The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganisms for its antimicrobial activities using standard procedures. The phytochemical analysis revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, carbohydrates, glycosides, sterols, etc. In the DPPH assay, the aqueous methanol fraction was found to be the most effective among all the fractions in comparison to the ascorbic acid standard. Using the zone of inhibition as inhibitory parameter, the crude methanol extract exhibited the best antibacterial activity when challenged against all the clinical isolates except Klebsiella pneumoniae . The aqueous methanol extract exhibited the best percentage fungal inhibition when compared to other fractions. The standard drugs ciprofloxacin and fluconazole exhibited a near 100% activity except with Proteus vulgaris where the crude methanol extract has a higher value.This research holds promise for the exploration of various potentially active secondary metabolites which would help in developing pharmaceuticals, especially antioxidant and antimicrobial drugs. The isolation and characterization of the exact metabolites responsible for these activities is therefore recommended.
Cervicovaginal Microbiota Predicts Neisseria gonorrhoeae Clinical Presentation

Neisseria gonorrhoeae infection of the female lower genital tract can present with a spectrum of phenotypes ranging from asymptomatic carriage to symptomatic cervical inflammation, or cervicitis. The factors that contribute to the development of asymptomatic or symptomatic infections are largely uncharacterized. We conducted a pilot study to assess differences in the cervicovaginal microbial community of patients presenting with symptomatic vs. asymptomatic N. gonorrhoeae infections to a sexually transmitted infections (STI) clinic. DNA was isolated from cervicovaginal swab specimens from women who tested positive for N. gonorrhoeae infection using a clinical diagnostic nucleic acid amplification test. We performed deep sequencing of 16S ribosomal RNA gene amplicons, followed by microbiome analyses with QIIME, and species-specific real-time PCR to assess the composition of microbial communities cohabitating the lower genital tract with the infecting N. gonorrhoeae. Specimens collected from asymptomatic individuals with N. gonorrhoeae infection and no co-infection with Chlamydia trachomatis and/or Trichomonas vaginalis carried Lactobacillus-dominant microbial communities more frequently than symptomatic patients without co-infection. When compared to asymptomatic individuals, symptomatic women had microbial communities characterized by more diverse and heterogenous bacterial taxa, typically associated with bacterial vaginosis (BV) [Prevotella, Sneathia, Mycoplasma hominis, and Bacterial Vaginosis-Associated Bacterium-1 (BVAB1)/“Candidatus Lachnocurva vaginae”]. Both symptomatic and asymptomatic N. gonorrhoeae patients with additional STI co-infection displayed a BV-like microbial community. These findings suggest that Lactobacillus-dominant vaginal microbial community may protect individuals from developing symptoms during lower genital tract infection with N. gonorrhoeae.
Longitudinal changes in home confinement and mental health implications: a 17-month follow-up study in England during the COVID-19 pandemic

Abstract Background The coronavirus disease 2019 (COVID-19) pandemic has brought about significant behavioural changes, one of which is increased time spent at home. This could have important public health implications. This study aimed to explore longitudinal patterns of ‘home confinement’ (defined as not leaving the house/garden) during the COVID-19 pandemic, and the associated predictors and mental health outcomes. Methods Data were from the UCL COVID-19 Social Study. The analytical sample consisted of 25 390 adults in England who were followed up for 17 months (March 2020–July 2021). Data were analysed using growth mixture models. Results Our analyses identified three classes of growth trajectories, including one class showing a high level of persistent home confinement (the home-confined, 24.8%), one changing class with clear alignment with national containment measures (the adaptive, 32.0%), and one class with a persistently low level of confinement (the unconfined, 43.1%). A range of factors were associated with the class membership of home-confinement trajectories, such as age, gender, income, employment status, social relationships and health. The home-confined class had the highest number of depressive (diff = 1.34–1.68, p < 0.001) and anxiety symptoms (diff = 0.84–1.05, p < 0.001) at the end of the follow-up than the other two classes. Conclusions There was substantial heterogeneity in longitudinal patterns of home confinement during the COVID-19 pandemic. People with a persistent high level of confinement had the worst mental health outcomes, calling for special attention in mental health action plans, in particular targeted interventions for at-risk groups.
Screening and characterization of thermophiles from Malacca hot spring

Seven strains of thermophilic bacteria (GD-1, GD-2, GD-3, GD-4, GD-5, GD-6 and GD-7) and three strains of thermophilic bacteria (JS-1, JS-2 and JS-3) were isolated and cultivated from samples collected from Kg Ganun Gadek, Alor Gajah and Kg Air Panas, Bemban hot springs respectively which both located at Malacca. The temperature and pH of the Kg Ganun Gadek sampling sites were 55°C and 7.59 respectively while 52 °C and 7.7 for Kg Air Panas Sampling sites. Thermus medium was found to be the most suitable medium for the growth for the eight strains. Strains GD-1, GD-2, GD-3 and GD 5 formed yellow colonies while strains GD-4, GD-6, JS-1, JS-2 and JS-3 formed white colonies. Further studies were done for strains GD-1, GD-2, GD-3, GD-4, GD-5, JS-1, JS-2 and JS-3. Cells of these strains were rod-shaped and occurred in single and chain like arrangement. Only two strains were Gram-negative (JS-2 and JS-3). Strains GD-1, GD-2 and GD-6 were able to hydrolyse starch. Catalase test revealed that strains GD-1, GD-2, GD-3, GD-6, JS-1 and JS-2 able to degraded hydrogen peroxide (H2O2) to water and oxygen. Blast search results of partial 16S rRNA sequences revealed strains GD-1, GD-2 and GD-6 to be Anoxybacillus gonesis while strains GD-3 and GD-5 are Anoxybacillus sp. Strain GD-4 showed closest to Bacillus licheniformis. Strain JS-1 showed closest relationship to Bacillus sp. and Geobacillus kautophilus while the result showed strain JS-3 is the most similar to Bacillus licheniformis and Bacillus sp. All result has 99% identity. The tentative identities of the strain were supported by the phenotypic and phylogenetic evidence.
Colloidal Crystals of NaYF4 Upconversion Nanocrystals Studied by Small‐Angle X‐Ray Scattering (SAXS)

Spherical NaYF4 upconversion nanocrystals with mean radii of about 5 and 11 nm are observed to form colloidal crystals, i.e., 3D assemblies of the particles with long‐range order. The colloidal crystals of the larger particles form directly in solution when dispersions of the particles in toluene are stored at room temperature for several weeks. Crystallization of the smaller particles takes place when their dispersions in hexane are slowly dried at elevated temperatures. The formation and the structure of the colloidal crystals are studied by small‐angle X‐ray scattering (SAXS). SAXS measurements show that the smaller as well as the larger particles assemble into a face‐centered cubic lattice with unit cell dimensions of a = 18.7 nm and a = 35.5 nm, respectively. The SAXS data also show that the particles in the colloidal crystals still bear a layer of oleic acid on their surfaces. The thickness of this layer is 1.5–1.8 nm, as determined by comparing the unit cell dimensions of the colloidal crystals with the mean particle sizes. The latter could be very precisely determined from the distinct oscillations observed in the SAXS data of dilute colloidal dispersions of the nanocrystals.
Decomposition and nutrient release pattern of animal manures biodegraded by fly larvae in Acrisols

Abstract: This study aims to assess the decomposition of manure biodegraded by fly larvae and the nutrient mineralization rates to understand the efficiency of the biodegraded manures for further use as soil amendment. A litter bag experiment was carried out over 75 d in an Acrisol in Benin using poultry manure, pig manure, mixture of poultry and sheep manure, mixture of poultry and cow manure, and mixture of poultry and pig manure, biodegraded by Musca domestica larvae. Nutrients content in the manures during the different stages of decomposition was analyzed. The mono-component exponential model Yt ? =? Y 0 × e-kt best described the manure decomposition and nutrients mineralization. The manures decomposed fast in the soil, and their nutrients were released fast in the poultry manure, the mixture of poultry and pig manure, and the mixture of poultry and cow manure. Nutrient mineralization increased in the order of P < N < K or N < P < K. Biodegradation of animal manures by fly larvae produced high-quality organic fertilizer through fast N and P release. This could aid in reducing the quantities of these elements applied as mineral fertilizer by farmers for sustaining agricultural soil productivity.
Ozone trend profiles in the stratosphere: combining ground-based data over Central Europe to consider uncertainties

Abstract. Observing stratospheric ozone is essential to assess if the Montreal Protocol has succeeded to save the ozone layer by banning ozone depleting substances. Recent studies have reported positive trends indicating that ozone is recovering in the upper stratosphere at mid-latitudes, but the trend magnitudes differ and uncertainties are still high. Trends and their uncertainties are influenced by factors such as instrumental drifts, sampling patterns, discontinuities, biases, or short-term anomalies that all might mask a potential ozone recovery. The present study investigates how anomalies, temporal measurement sampling rates and trend period lengths influence resulting trends. We present an approach for handling suspicious anomalies in trend estimations to improve the derived trend profiles. The approach was applied to data from a Ground-based Millimetre-wave Ozone Spectrometer (GROMOS) located in Bern, Switzerland. We compare our improved GROMOS trend estimate with results from other ground stations (lidars, ozonesondes, and microwave radiometers) in Central Europe. The data indicate positive trends of 1 to 3 % per decade at an altitude of about 40 km (3 hPa), providing a confirmation of ozone recovery in the upper stratosphere in agreement to satellite observations. At lower altitudes, the ground station data show inconsistent trend results, which emphasize the importance of ongoing research on lower stratospheric ozone trends. Our presented method of a combined analysis of ground station data provides a useful approach to recognize and to reduce uncertainties in stratospheric ozone trends by considering anomalies in the trend estimation. We conclude that stratospheric trend estimations still need improvement and that our approach provides a tool that can also be useful for other data sets.
Theoretical Study and Experimental Validation of Energetic Performances of Photovoltaic/Thermal Air Collector

This work undertakes both simulation and experimental studies of a new design of a photovoltaic thermal solar air collector (PV/T). In order to improve the thermal and electrical performances for a specific application, the analytical expressions for thermal parameters and efficiency are derived by developing an energy balance equation for each component of the PV/T air collector. This type of hybrid collector can be applied in the facades of buildings. The electricity and heat produced will satisfy the energy needs of the buildings, while ensuring an aesthetic view of its facades. A typical prototype was designed, constructed, and implemented in the applied research unit on renewable energies in Ghardaia, situated in the south of Algeria. This region has semiarid characteristics. Results obtained by an experimental test are presented and compared to those predicted through simulation. Results include the temperature of each component of the PV/T collector and air temperature at the inlet and outlet of the channel. It has been found that the theoretical results predicted by the developed mathematical model, for instance, outlet temperature, agree with those found through experimental work.