Miracle-dz/UniFAR-FTUs huggingface dataset:
Increased maternal care rescues altered reinstatement responding following moderate prenatal alcohol exposure.

BACKGROUND Fetal Alcohol Spectrum Disorders (FASD) commonly includes deficits in learning, memory and executive control that can have a severe negative impact on quality of life across the lifespan. It is still unclear how prenatal alcohol exposure (PAE) affects executive control processes, such as control over reward seeking, that lead to inappropriate behavior later in life. Learning and reinstatement of a previously learned response after extinction is a simple, well validated, measure of both acquisition of a rewarded instrumental response, and sensitivity to reward and reward-associated cues. We investigated the effects of PAE on learning, extinction and reinstatement of a simple instrumental response for food reward. Next, we assessed the effectiveness of an early intervention, communal nest (CN) housing, on increased reinstatement of an extinguished response seen after PAE. METHODS To assess the effects of PAE on control over reward seeking, we tested male and female PAE and saccharine (SAC) controls raised in a standard nest (SN) on the acquisition, extinction, and food reward induced reinstatement of an instrumental response utilizing a touch-screen based paradigm. Next, in order to examine the effects of an early life intervention on these behaviors, we tested PAE and SAC mice raised in a communal nest (CN) early life environment on these behaviors. RESULTS PAE mice readily acquired and extinguished a simple touch response to a white square stimulus. However, PAE mice showed significantly increased and persistent reinstatement compared to controls. Increased maternal care via rearing in CN slowed acquisition and sped extinction learning, and rescued the significantly increased reinstatement responding in PAE mice. CONCLUSIONS Together these results demonstrate that even moderate PAE is sufficient to alter control over reward seeking as measured by reinstatement. Importantly, an early life intervention previously shown to improve cognitive outcomes in PAE mice was sufficient to ameliorate this effect. This article is protected by copyright. All rights reserved.
TMEM16B Calcium-Activated Chloride Channels Regulate Action Potential Firing in Lateral Septum and Aggression in Male Mice

The lateral septum (LS) plays an important role in regulating aggression. It is well recognized that LS lesions lead to a dramatic increase in aggressive behaviors. A better understanding of LS neurophysiology and its functional output is therefore important to assess LS involvement in regulating aggression. The LS is a heterogeneous structure that maintains inputs and outputs with multiple brain regions, and is also divided into subregions that innervate one another. Thus, it is challenging to identify the exact cell type and projections for characterization. In this study, we determined the expression pattern of the calcium-activated chloride channel, TMEM16B, in the LS of both male and female mice. We then investigated the physiological contribution of the calcium-activated chloride channel to LS neuronal signaling. By performing whole-cell patch-clamp recording, we showed that TMEM16B alters neurotransmitter release at the hippocampal-LS synapse, and regulates spike frequency and spike frequency adaptation in subpopulations of LS neurons. We further demonstrated that loss of TMEM16B function promotes lengthened displays of aggressive behaviors by male mice during the resident intruder paradigm. In conclusion, our findings suggest that TMEM16B function contributes to neuronal excitability in subpopulations of LS neurons and the regulation of aggression in male mice. SIGNIFICANCE STATEMENT Aggression is a behavior that arose evolutionarily from the necessity to compete for limited resources and survival. One particular brain region involved in aggression is the lateral septum (LS). In this study, we characterized the expression of the TMEM16B calcium-activated chloride channel in the LS and showed that TMEM16B regulates the action potential firing frequency of LS neurons. We discovered that loss of TMEM16B function lengthens the displays of aggressive behaviors in male mice. These findings suggest that TMEM16B plays an important role in regulating LS neuronal excitability and behaviors associated with LS function, thereby contributing to our understanding of how the LS may regulate aggression.
Participant Engagement and Ethical Digital Storytelling: The MOCHA Moving Forward Study

African-American men continue to bear a disproportionate share of the burden of health disparities, in general, and chronic diseases, in particular. The Men of Color Health Awareness (MOCHA) Moving Forward study seeks to determine the effectiveness of an innovative, community-driven program to improve the health and quality of life of low-income African-American men between the ages of 35 to 70 years by reducing identified social risk factors for chronic disease for these men. The project uses digital storytelling (DST) to encourage African-American men to tell their stories, especially related to stress, gender role stereotypes, and mental and physical health and well-being. Thirty-six men were recruited to participate in one of four DST workshops, which resulted in each participant creating a 2- to 3-minute digital story. In this article, we describe and analyze three salient ethical dilemmas that arose in conducting the Men of Color Health Awareness Moving Forward study DST workshops with African-American men. The dilemmas can be traced to the distinct purposes for which DST can be used, data collection or intervention development, and the trade-offs between protecting and patronizing participants. We discuss potential ways to resolve or circumvent the identified issues.
Perspectives on HIV partner notification, partner HIV self‐testing and partner home‐based HIV testing by pregnant and postpartum women in antenatal settings: a qualitative analysis in Malawi and Zambia

HIV testing male partners of pregnant and postpartum women can lead to improved health outcomes for women, partners and infants. However, in sub‐Saharan Africa, few male partners get HIV tested during their partner's pregnancy in spite of several promising approaches to increase partner testing uptake. We assessed stakeholders’ views and preferences of partner notification, home‐based testing and secondary distribution of self‐test kits to understand whether offering choices for partner HIV testing may increase acceptability.
Approximate-SAD Circuit for Power-efficient H.264 Video Encoding under Maintaining Output Quality and Compression Efficiency

We develop a novel SAD circuit for power-efficient H.264 encoding, namely a-SAD. Here, some highest-order MSB’s are approximated to single MSB. Our theoretical estimations show that our proposed design simultaneously improves performance and power of SAD circuit, achieving good power efficiency. We decide that the optimal number of approximated MSB’s is four under 8-bit YUV-420 format, the largest number not to affect video quality and compression-rate in our video experiments. In logic simulations, our a-SAD circuit shows at least 9.3% smaller critical-path delay compared to existing SAD circuits. We compare power dissipation under iso-throughput scenario, where our a-SAD circuit obtains at least 11.6% power saving compared to other designs. We perform same simulations under two-and three-stage pipelined architecture. Here, our a-SAD circuit delivers significant performance (by 13%) and power (by 17% and 15.8% for two and three stages respectively) improvements.
Proteostasis is essential during cochlear development for neuron survival and hair cell polarity

Protein homeostasis is essential to cell function, and a compromised ability to reduce the load of misfolded and aggregated proteins is linked to numerous age‐related diseases, including hearing loss. Here, we show that altered proteostasis consequent to Elongator complex deficiency also impacts the proper development of the cochlea and results in deafness. In the absence of the catalytic subunit Elp3, differentiating spiral ganglion neurons display large aggresome‐like structures and undergo apoptosis before birth. The cochlear mechanosensory cells are able to survive proteostasis disruption but suffer defects in polarity and stereociliary bundle morphogenesis. We demonstrate that protein aggregates accumulate at the apical surface of hair cells, where they cause a local slowdown of microtubular trafficking, altering the distribution of intrinsic polarity proteins and affecting kinocilium position and length. Alleviation of protein misfolding using the chemical chaperone 4‐phenylbutyric acid during embryonic development ameliorates hair cell polarity in Elp3‐deficient animals. Our study highlights the importance of developmental proteostasis in the cochlea and unveils an unexpected link between proteome integrity and polarized organization of cellular components.
Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits

In some induced-ovulating species, beta nerve growth factor (β-NGF) has important roles in ovulation, though data for rabbits are still inconclusive. In this study we first synthesized functional recombinant β-NGF from rabbit tissue (rrβ-NGF) to address the following objectives: 1) to compare rabbit β-NGF amino acid sequence with those of other induced- or spontaneous-ovulating species; 2) to assess the effects of rrβ-NGF on rabbit sperm viability and motility, and 3) to examine the in vivo ovulation inducing effect of rrβ-NGF added to the seminal dose in rabbit does. The NGF gene in rabbit prostate tissue was sequenced by Rapid Amplification of cDNA Ends and annotated in GenBank (KX528686). Recombinant rβ-NGF was produced in CHO cells and purified by affinity chromatography. Once confirmed by Western blotting and mass spectrometry (MALDI-TOF) that the amino acid sequence of the recombinant protein corresponded to β-NGF, its functionality was validated in PC12 cells in a successful dose-response study over 8 days. The amino acid sequence of prostate rabbit NGF differed to that of other species mainly in its receptor binding sites. In all the spontaneous ovulating species examined, compared with rabbit, alanine and proline residues, which interact with the high-affinity receptor, were replaced by a serine. In rabbits, asparagine and methionine were substituted by lysine at the low-affinity receptor binding site. In time- and dose-response experiments, the in vitro addition of rrβ-NGF to the ejaculate did not affect sperm viability whereas sperm motility parameters were enhanced by the addition of 1 μg/mL of the neuropeptide. Addition of this same concentration of rrβ-NGF to the seminal dose administered via the intravaginal route in does induced ovulation with a delayed LH peak, leading to a plasma progesterone increase, gestation and delivery. Our findings suggest that rrβ-NGF could be a useful option for biotechnological and reproduction assisted techniques in rabbits but further studies are needed.
Development of an Embedded Myokinetic Prosthetic Hand Controller

The quest for an intuitive and physiologically appropriate human machine interface for the control of dexterous prostheses is far from being completed. In the last decade, much effort has been dedicated to explore innovative control strategies based on the electrical signals generated by the muscles during contraction. In contrast, a novel approach, dubbed myokinetic interface, derives the control signals from the localization of multiple magnetic markers (MMs) directly implanted into the residual muscles of the amputee. Building on this idea, here we present an embedded system based on 32 magnetic field sensors and a real time computation platform. We demonstrate that the platform can simultaneously localize in real-time up to five MMs in an anatomically relevant workspace. The system proved highly linear (R2 = 0.99) and precise (1% repeatability), yet exhibiting short computation times (4 ms) and limited cross talk errors (10% the mean stroke of the magnets). Compared to a previous PC implementation, the system exhibited similar precision and accuracy, while being ~75% faster. These results proved for the first time the viability of using an embedded system for magnet localization. They also suggest that, by using an adequate number of sensors, it is possible to increase the number of simultaneously tracked MMs while introducing delays that are not perceivable by the human operator. This could allow to control more degrees of freedom than those controllable with current technologies.
Risk factor analysis for the development and progression of retinopathy of prematurity

Purpose To classify the risk factors that contribute to the development versus progression of retinopathy of prematurity (ROP). Methods The medical records of premature infants born with a birth weight (BW) less than 1501 g or a gestational age (GA) of 32 weeks or less were retrospectively reviewed. Twenty potential risk factors that may influence the development or progression of ROP were analyzed by univariate and multivariate logistic regression analyses. The progression of ROP was defined as type 1 ROP, threshold ROP, or aggressive posterior ROP for which treatment was recommended. Results A total of 324 eyes were included; 157 eyes (48.5%) showed ROP development, and 48 eyes exhibited ROP progression (14.8% of all eyes and 30.6% of the ROP-developed eyes). According to the univariate and multivariate logistic regression analyses, prenatal steroid use, GA, the duration of mechanical ventilation, and respiratory distress syndrome were associated with the development of ROP. However, GA, bronchopulmonary dysplasia, the number of red blood cell units transfused, intraventricular hemorrhage, and periventricular leukomalacia were significantly correlated with ROP progression. Conclusion The risk factors that influenced ROP development versus ROP progression were not identical. Evaluating these risk factors during screening of high-risk premature infants will help determine the appropriate timing of examinations and treatment.
Adjuvant Effect of Molecular Iodine in Conventional Chemotherapy for Breast Cancer. Randomized Pilot Study

This study analyzes an oral supplement of molecular iodine (I2), alone and in combination with the neoadjuvant therapy 5-fluorouracil/epirubicin/cyclophosphamide or taxotere/epirubicin (FEC/TE) in women with Early (stage II) and Advanced (stage III) breast cancer. In the Early group, 30 women were treated with I2 (5 mg/day) or placebo (colored water) for 7–35 days before surgery. For the Advanced group, 30 patients received I2 or placebo, along with FEC/TE treatment. After surgery, all patients received FEC/TE + I2 for 170 days. I2 supplementation showed a significant attenuation of the side effects and an absence of tumor chemoresistance. The control, I2, FEC/TE, and FEC/TE + I2 groups exhibited response rates of 0, 33%, 73%, and 100%, respectively, and a pathologic complete response of 18%, and 36% in the last two groups. Five-year disease-free survival rate was significantly higher in patients treated with the I2 supplement before and after surgery compared to those receiving the supplement only after surgery (82% versus 46%). I2-treated tumors exhibit less invasive potential, and significant increases in apoptosis, estrogen receptor expression, and immune cell infiltration. Transcriptomic analysis indicated activation of the antitumoral immune response. The results led us to register a phase III clinical trial to analyze chemotherapy + I2 treatment for advanced breast cancer.
Role-Based Access Control Model for Workflow Systems

Nowadays workflow is coming into more and more notice in many areas such as OA, e-government, e-business. Security is a important problem in workflow environment. Access control is a vital component of Security. In this paper, a RBAC model in workflow environment (WRBAC) is introduced. The model is based on proposed NIST standard for RBAC, formally describes the relationship between the key elements of access control in workflow systems such as user, role, permission and activity, presents the static and dynamic constraints. The model can effectively reduce the risk of information leakage and fraud, meet the requirements for access control in workflow systems.
Dietary nitrate supplementation enhances cerebrovascular CO2 reactivity in a sex-specific manner.

Insufficient nitric oxide (NO) bioavailability plays an important role in endothelial dysfunction and increased NO has the potential to enhance cerebral blood flow (CBF). Dietary supplementation with sodium nitrate, a precursor of NO, could improve cerebrovascular function but this has not been investigated. In seventeen individuals, we examined the effects of a 7-day supplementation of dietary nitrate (0.1mmol/kg/day) on cerebrovascular function using a randomised, single-blinded placebo-controlled crossover design. We hypothesized that 7-day dietary nitrate supplementation increases CBF response to CO2 (cerebrovascular CO2 reactivity) and cerebral autoregulation (CA). We assessed middle cerebral artery blood velocity (MCAv) and blood pressure (BP) at rest and during CO2 breathing. Transfer function analysis was performed on resting beat-to-beat MCAv and BP to determine CA, from which phase, gain and coherence of the BP-MCAv data were derived. Dietary nitrate elevated plasma nitrate concentration by ˜420% (P<0.001) and lowered gain (d = 1.2, P = 0.025) and phase of the BP-MCAv signal compared to placebo treatment (d=0.7, P=0.043), while coherence was unaffected (P=0.122). Dietary nitrate increased the MCAv-CO2 slope in a sex-specific manner (interaction: P=0.016). Dietary nitrate increased the MCAv-CO2 slope in males (d=1.0, P=0.014 vs. placebo), but had no effect in females (P=0.919). Our data demonstrate that dietary nitrate greatly increased cerebrovascular CO2 reactivity in healthy individuals, while its effect on CA remains unclear. The selective increase in the MCAv-CO2 slope observed in males indicates a clear sexual dimorphic role of NO in cerebrovascular function.
Association Between Proxy- or Self-Reported Cognitive Decline and Cognitive Performance in Memory Clinic Visitors.

BACKGROUND It is uncertain whether self- and proxy-reported cognitive decline in older adults reflect an actual objective cognitive dysfunction in the clinical sense, and if these are predictive for developing dementia. OBJECTIVE The aim of the present study is to investigate the cross-sectional and longitudinal relation between subjective cognitive decline and objective cognitive performance, depressive symptoms, and to determine the predictive value for development of dementia. METHODS We included 405 patients without dementia at first visit from the Maastricht memory clinic participating in a longitudinal cohort study. Subjective cognitive decline was measured using a self- and proxy-report questionnaire. All patients underwent a standardized neuropsychological assessment. Follow-up assessments were performed yearly for three consecutive years, and once after five years. RESULTS Subjective cognitive decline was associated with lower cognitive performance and more depressive symptoms. When comparing self- (n = 342, 84% ) and proxy-reported decline (n = 110, 27% ), it was shown that proxy reports were associated with a more widespread pattern of lower cognitive performance. In participants without cognitive impairment proxy-reported decline was not associated with depressive symptoms. In contrast, self-reported decline was associated with a stable course of depressive symptoms at follow-up. Proxy-reported cognitive decline (HR = 1.76, 95% CI = 1.12- 2.78), and mutual complaints (HR = 1.73, CI:1.09- 2.76) predicted incident dementia while self-reported decline did not reach statistical significance (HR = 1.26, 95% CI = 0.65- 2.43). CONCLUSION Proxy-reported cognitive decline was consistently associated with lower cognitive performance and conversion to dementia over 5 years. Self-reported cognitive decline in patients without cognitive impairment might indicate underlying depressive symptoms and thus deserve clinical attention as well.
Children’s Fear and Pain During Medical Procedures: A Quality Improvement Study With a Humanoid Robot

Background Pediatric patients undergo a variety of painful medical procedures. Purpose The goal of this quality improvement study was to introduce a humanoid robot (MEDi®) programmed with strategies, such as distraction and deep breathing, at inpatient and outpatient units to determine any preliminary effects on children’s pain and fear during medical procedures. Methods A nonrandomized two-group pre- and posttest design was used to compare pain and fear of children before and after intervention versus standard care. A total of 46 children aged 2–15 years undergoing various medical procedures in a pediatric hospital, and their parents completed the Children’s Fear Scale and the Faces Pain Scale-Revised. The former was used both before and after the procedure, while the latter only after the procedure. Results Children (n = 18), who interacted with the robot before and during a procedure, and their parents reported significantly lower levels of fear and pain than did children (n = 28) and their parents in standard care, ps < . 05. Conclusions The use of a humanoid robot programmed with psychological strategies to support coping may enhance children’s experiences of care for pain management.
Continuous Monitoring of Soil Nitrate Using a Miniature Sensor with Poly(3-octyl-thiophene) and Molybdenum Disulfide Nanocomposite.

There is an unmet need for improved fertilizer management in agriculture. Continuous monitoring of soil nitrate would address this need. This paper reports an all-solid-state miniature potentiometric soil sensor that works in direct contact with soils to monitor nitrate-nitrogen (NO3--N) in soil solution with parts-per-million (ppm) resolution. A working electrode is formed from a novel nanocomposite of poly(3-octyl-thiophene) and molybdenum disulfide (POT-MoS2) coated on a patterned Au electrode and covered with a nitrate-selective membrane using a robotic dispenser. The POT-MoS2 layer acts as an ion-to-electron transducing layer with high hydrophobicity and redox properties. The modification of the POT chain with MoS2 increases both conductivity and anion exchange, while minimizing the formation of a thin water layer at the interface between the Au electrode and the ion-selective membrane, which is notorious for solid-state potentiometric ion sensors. Therefore, the use of POT-MoS2 results in an improved sensitivity and selectivity of the working electrode. The reference electrode comprises a screen-printed silver/silver chloride (Ag/AgCl) electrode covered by a protonated Nafion layer to prevent chloride (Cl-) leaching in long-term measurements. This sensor was calibrated using both standard and extracted soil solutions, exhibiting a dynamic range that includes all concentrations relevant for agricultural applications (1-1500 ppm NO3--N). With the POT-MoS2 nanocomposite, the sensor offers a sensitivity of 64 mV/decade for nitrate detection, compared to 48 and 38 mV/decade for POT and MoS2 alone, respectively. The sensor was embedded into soil slurries where it accurately monitored nitrate for a duration of 27 days.