Miracle-dz/UniFAR-FTUs huggingface dataset:
Cellular context determines primary characteristics of human TRPC5 as a cold‐activated channel

The human transient receptor potential canonical 5 (TRPC5) is a calcium‐permeable, nonselective cation channel expressed in the central and peripheral nervous system and also in other tissues such as the kidney, synovium, and odontoblasts. TRPC5 has been recently confirmed to play a key role in spontaneous, inflammatory mechanical, and cold pain. Although TRPC5 activation is known to be cold sensitive, it is unclear whether this property is intrinsic to the channel protein and whether or to what extent it may be determined by the cellular environment. In this study, we explored the cold sensitivity of human TRPC5 at the single‐channel level using transiently transfected HEK293T cells. Upon decreasing the temperature, the channel demonstrated prolonged mean open dwell times and a robust increase in the open probability (Po), whereas the amplitude of unitary currents decreased ~1.5‐fold per 10°C of temperature difference. In the absence of any agonists, the temperature dependence of Po was sigmoidal, with a steep slope within the temperature range of 16°C–11°C, and exhibited saturation below 8–5°C. Thermodynamic analysis revealed significant changes in enthalpy and entropy, suggesting that substantial conformational changes accompany cold‐induced gating. The mutant channel T970A, in which the regulation downstream of G‐protein coupled receptor signaling was abrogated, exhibited higher basal activity at room temperature and a less steep temperature response profile, with an apparent threshold below 22°C. An even more pronounced decrease in the activation threshold was observed in a mutant that disrupted the electrostatic interaction of TRPC5 with the endoplasmic reticulum calcium sensor stromal interaction molecule 1. Thus, TRPC5 exhibits features of an intrinsically cold‐gated channel; its sensitivity to cold tightly depends on the phosphorylation status of the protein and intracellular calcium homeostasis.
Estudo com idosos de instituições asilares no município de Natal/RN: características socioeconômicas e de saúde

O envelhecimento populacional acelerado no Brasil certamente aumentara o numero de idosos institucionalizados. Baseados nesse enfoque, este estudo exploratorio descritivo foi desenvolvido em tres instituicoes asilares na cidade de Natal (RN) e objetivou caracterizar o idoso asilado; identificar os problemas socioeconomicos, de saude e as causas que os levaram ao asilo. A amostra constituiu-se de 30% da totalidade de idosos de cada instituicao, as quais tem carater filantropico e abrigam idosos carentes. Os resultados mostraram que as tres instituicoes possuem caracteristicas socioeconomicas e de saude semelhantes as encontradas na literatura, pontuando-se baixas condicoes financeiras, contato familiar conflituoso, atividades de lazer limitadas ou ausentes, saude precaria, restrito atendimento medico e de enfermagem, alem da ausencia de planos privados de saude. Conclui-se, refletindo-se quanto as condicoes socioeconomicas e de saude desses idosos, que ha a necessidade da atuacao de orgaos competentes com vistas a seus direitos de cidadania.
The association between maternal perinatal mental health and perfectionism: A systematic review and meta‐analysis

Abstract Background Perfectionism is an important feature of adult psychopathology. In the absence of a prior review of the role of perfectionism in perinatal psychopathology, we aimed to ascertain whether perfectionism was associated with symptoms of maternal perinatal depression and anxiety. Method We followed PRISMA guidance (PROSPERO: 42019143369), estimated weighted effect sizes and tested possible moderators: timing (pre or post‐ natal), scales used to measure constructs, infant gender, temperament and age; and rated study quality. Results Fourteen studies met eligibility criteria. Perfectionism as a whole, and the perfectionistic concerns dimension, were moderately correlated with common maternal perinatal mental health difficulties r = . 32 (95% Confidence Interval = 0.23 to 0.42). In sub‐group analyses, perfectionistic concerns were associated with depression (r = .35, 95% CI = 0.26–0.43). We found no evidence of significant moderation of associations. Limitations Included studies had methodological and conceptual limitations. All studies examined depression and two examined anxieties; all examined perfectionistic concerns and four examined perfectionist strivings. Conclusions Perfectionism, namely perfectionistic concerns, is potentially associated with common maternal perinatal mental health problems. While further research is warranted, identification of perfectionism in the perinatal period may help focus resources for intervention, reducing the prevalence of perinatal mental health difficulties.
Opportunities and Challenges When Using the Electronic Health Record for Practice-Integrated Patient-Facing Interventions: The e-Assist Colon Health Randomized Trial

Background Even after a physician recommendation, many people remain unscreened for colorectal cancer (CRC). The proliferation of electronic health records (EHRs) and tethered online portals may afford new opportunities to embed patient-facing interventions within clinic workflows and engage patients following a physician recommendation for care. We evaluated the effectiveness of a patient-facing intervention designed to complement physician office-based recommendations for CRC screening. Design Using a 2-arm pragmatic, randomized clinical trial, we evaluated the intervention’s effect on CRC screening use as documented in the EHR (primary outcome) and the extent to which the intervention reached the target population. Trial participants were insured, aged 50 to 75 y, with a physician recommendation for CRC screening. Typical EHR functionalities, including patient registries, health maintenance flags, best practice alerts, and secure messaging, were used to support research-related activities and deliver the intervention to enrolled patients. Results A total of 1,825 adults consented to trial participation, of whom 78% completed a baseline survey and were exposed to the intervention. Most trial participants (>80%) indicated an intent to be screened on the baseline survey, and 65% were screened at follow-up, with no significant differences by study arm. One-third of eligible patients were sent a secure message. Among those, more than three-quarters accessed study material. Conclusions By leveraging common EHR functionalities, we integrated a patient-facing intervention within clinic workflows. Despite practice integration, the intervention did not improve screening use, likely in part due to portal-based interventions not reaching those for whom the intervention may be most effective. Implications Embedding patient-facing interventions within the EHR enabled practice integration but may minimize program effectiveness by missing important segments of the patient population. Highlights Electronic health record tools can be used to facilitate practice-embedded pragmatic trial and patient-facing intervention processes, including patient identification, study arm allocation, and intervention delivery. The online portal-embedded intervention did not improve colorectal cancer (CRC) screening uptake following a physician recommendation, likely in part because portal users tend to be already highly engaged with healthcare. Relying on patient portals alone for CRC screening interventions may not alter screening use and could exacerbate well-known care disparities.
Synchrotron X‐ray Electron Density Analysis of Chemical Bonding in the Graphitic Carbon Nitride Precursor Melamine

Abstract Melamine is a precursor and building block for graphitic carbon nitride (g‐CN) materials, a group of layered materials showing great promise for catalytic applications. The synthetic pathway to g‐CN includes a polycondensation reaction of melamine by evaporation of ammonia. Melamine molecules in the crystal organize into wave‐like planes with an interlayer distance of 3.3 Å similar to that of g‐CN. Here we present an extensive investigation of the experimental electron density of melamine obtained from modelling of synchrotron radiation X‐ray single‐crystal diffraction data measured at 25 K with special focus on the molecular geometry and intermolecular interactions. Both intra‐ and interlayer structures are dominated by hydrogen bonding and π‐interactions. Theoretical gas‐phase optimizations of the experimental molecular geometry show that bond lengths and angles for atoms in the same chemical environment (C−N bonds in the ring, amine groups) differ significantly more for the experimental geometry than for the gas‐phase‐optimized geometries, indicating that intermolecular interactions in the crystal affects the molecular geometry. In the experimental crystal geometry, one amine group has significantly more sp3‐like character than the others, hinting at a possible formation mechanism of g‐CN. Topological analysis and energy frameworks show that the nitrogen atom in this amine group participates in weak intralayer hydrogen bonding. We hypothesize that melamine condenses to g‐CN within the layers and that the unique amine group plays a key role in the condensation process.
Substance-related diagnosis type predicts the likelihood and co-occurrence of preterm and cesarean delivery

Abstract Objective: This article aimed to evaluate whether a substance-related diagnosis (SRD; i.e., alcohol, opioids, cannabis, stimulants, nicotine) predicts the likelihood and co-occurrence of preterm (20–37 weeks’ gestation) and cesarean delivery. Methods: This study reviewed electronic health record data on women (aged 18–44 years) who delivered a single live or stillbirth at ≥ 20 weeks of gestation from 2012 to 2019. Women with and without an SRD were matched on key demographic characteristics at a 1:1 ratio. Adjusting for covariates, odds ratios and 95% confidence intervals were calculated. Results: Of the 19,346 deliveries, a matched cohort of 2,158 deliveries was identified. Of these, 1,079 (50%) had an SRD, 280 (13%) had a preterm delivery, 833 (39%) had a cesarean delivery, and 166 (8%) had a co-occurring preterm and cesarean delivery. An SRD was significantly associated with preterm and cesarean delivery (AOR = 1.84 [95% CI, 1.41–2.39], p-value= <0.0001; AOR = 1.51 [95% CI, 1.23–1.85], p-value= <0.0001). An alcohol-related diagnosis (AOR = 1.82 [95% CI, 1.01–3.28], p-value= 0.0471), opioid-related diagnosis (AOR = 1.94 [95% CI, 1.26–2.98], p-value= 0.0027), stimulant-related diagnosis (AOR = 1.65 [95% CI, 1.11–2.45], p-value= 0.0142), and nicotine-related diagnosis (AOR = 1.54 [95% CI, 1.05–2.26], p-value= 0.0278) were associated with co-occurring preterm and cesarean delivery. Conclusions: Pregnant women with an SRD experienced disproportionally higher odds of preterm and cesarean delivery compared to pregnant women without an SRD. Substance-type predicts the type of delivery outcome. An SRD in pregnant women should be identified early to reduce potential harm through intervention and treatment.
Multiple‐Resonance Extension and Spin‐Vibronic‐Coupling‐Based Narrowband Blue Organic Fluorescence Emitters with Over 30% Quantum Efficiency

Achieving narrow‐bandwidth emission and high external quantum efficiency (EQE) simultaneously is a challenge for next‐generation blue‐emitting organic light‐emitting diodes (OLEDs). In this study, novel multiple‐resonance thermally activated delayed fluorescence (MR‐TADF) emitters are developed by fusing an indolocarbazole unit with two carbazole skeletons using para‐oriented nitrogen atoms. The resulting rigid and planar π‐system without electron‐accepting atoms exhibits pure blue photoluminescence at 470 nm, reaching a 100% quantum yield with a full‐width‐at‐half‐maximum (FWHM) of 25 nm. Higher‐level quantum chemistry calculations confirm an MR effect within the extended π‐conjugation and an enhanced triplet‐to‐singlet crossover (104 s−1) through a reduced energy gap (ΔEST) coupled with large spin‐vibronic coupling mediated by low‐lying triplet excited states. An OLED fabricated using the MR‐TADF emitter with CIE color coordinates of (0.12, 0.16) exhibits a record high EQE of 30.9% and a small FWHM of 23 nm. With further optimization of the device structure, a high EQE of 33.8% is achieved without additional outcoupling enhancements owing to the near‐perfect horizontal alignment of the emitting dipoles.
Polarized trafficking and copper transport activity of ATP7B: A mutational approach to establish genotype–phenotype correlation in Wilson disease

Mutation in ATP7B gene causes Wilson disease (WD) that is characterized by severe hepatic and neurological symptoms. ATP7B localizes at the trans‐Golgi Network (TGN) transporting copper to copper‐dependent enzymes and traffics in apically targeted vesicles upon intracellular copper elevation. To decode the cellular underpinnings of WD manifestation we investigated copper‐responsive polarized trafficking and copper transport activity of 15 WD causing point mutations in ATP7B. Amino‐terminal mutations Gly85Val, Leu168Pro, and Gly591Asp displayed TGN and subapical localization whereas, Leu492Ser mislocalized at the basolateral region. The actuator domain mutation Gly875Arg shows retention in the endoplasmic reticulum (ER), Ala874Val and Leu795Phe show partial targeting to TGN and post‐Golgi vesicles. The nucleotide‐binding domain mutations His1069Gln and Leu1083Phe also display impaired targeting. The C‐terminal mutations Leu1373Pro/Arg is arrested at ER but Ser1423Asn shows TGN localization. Transmembrane mutant Arg778Leu resides in ER and TGN while Arg969Gln is exclusively ER localized. Cellular Cu level does not alter the targeting of any of the studied mutations. Mutants that traffic to TGN exhibits biosynthetic function. Finally, we correlated cellular phenotypes with the clinical manifestation of the two most prevalent mutations; the early onset and more aggressive WD caused by Arg778Leu and the milder form of WD caused by mutation His1069Gln.
New Evidence of Canthariphily: Tilloidea transversalis (Coleoptera: Cleridae) Sequestering Cantharidin From Lydus trimaculatus (Coleoptera: Meloidae)

Abstract Cantharidin (CTD) is a defensive compound autogenously and exclusively produced by two phylogenetically related beetle families: Meloidae and Oedemeridae. Although this molecule usually acts as a strong deterrent against potential predators and parasites, some arthropod species, collectively named ‘canthariphilous species’, are attracted to CTD. Some species can sequester CTD from the CTD-producing species, using it as a chemical defense against enemies. The present paper focuses on the first-ever description of canthariphilous interactions between a checkered beetle species (Coleoptera: Cleridae) and a CTD -producing species. Field observations revealed individuals of the phytophagous beetle Tilloidea transversalis (Charpentier, 1825) (Coleoptera: Cleridae) biting individuals of the blister beetle Lydus trimaculatus (Fabricius, 1775) (Coleoptera: Meloidae). Laboratory behavioral experiments followed to verify if this peculiar behavior of T. transversalis also occurs on other co-occurring species. Moreover, chemical analyses were performed to assess whether T. transversalis can sequester CTD. Our results show that T. transversalis only attacks CTD-producing species. However, while chemical analyses prove that T. transversalis can sequester CTD from the hemolymph of L. trimaculatus, some clues (based on a CTD-baited traps sampling) suggest that this beetle, contrarily to other canthariphilous species, does not appear to show a high attraction to pure synthetic CTD. Thus, other unknown signals, alone or in combination with CTD, could be implicated in triggering the canthariphilous behaviors of T. transversalis.
Differential Subsampling with Cartesian Ordering Contrast-Enhanced Magnetic Resonance Angiography for the Preoperative Assessment of Anterolateral Thigh Flap

Objective To investigate the clinical application of differential subsampling with Cartesian ordering (DISCO) contrast-enhanced (CE) magnetic resonance angiography for anterolateral thigh (ALT) flap transplantation, using operative findings as a reference. Materials and Methods Thirty patients (21 males and nine females; mean age ± standard deviation, 45.5 ± 15.6 years) who were scheduled to undergo reconstruction with ALT flaps between June 2020 and June 2021 were included in the prospective study. Before ALT flap transplantation, patients were scanned using CE-DISCO imaging. All acquired DISCO images of the 60 lower limbs (both sides from each patient) were analyzed using maximum intensity projection and volume rendering methods. Two experienced radiologists were employed to examine the patterns of the lateral circumflex femoral artery (LCFA), its branches, and perforators and their skin termini, which were compared with the operative findings. Results Using CE-DISCO, the patterns of the LCFA and its branches were clearly identified in all patients. Four different origins of the LCFA were found among the 60 blood vessels: type I (44/60, 73.3%), type II (6/60, 10.0%), type III (8/60, 13.3%), and type IV (2/60, 3.3%). Owing to a lack of perforators entering the skin, two patients did not undergo ALT flap transplantation. For the remaining 28 patients, the ALT flaps in 26 patients were successfully operated without flap re-selection during the operation, while the remaining two patients underwent other surgical procedures due to the thin diameter of the perforator or injury of the perforator during the operation. The success rate of flap transplantation was 92.8% (26/28). All transplanted flaps exhibited good blood supply and achieved primary healing without infection or delayed healing. Conclusion CE-DISCO imaging can be an effective method for preoperative perforator imaging before ALT flap transplantation.
“I need to be alert at night to provide care”: Factors associated with problematic sleep among young Australian caregivers

ABSTRACT Objectives There is increased recognition that young people (<25 years) may occupy a carer role for family or others with health conditions or disability. This is often in addition to study and social activities. This means competing demands on time, and insufficient sleep. Our aim was to determine the contribution of caring duties to problematic sleep in young carers. Methods A survey of Australian carers was conducted, including questions on demographics, characteristics of the carer and care recipient, and sleep quality and quantity. Participants were eligible if they reported sleep time <7 hr or dissatisfaction with their sleep, and were aged 15–24 years. Results A total of 110 participants (71.8%_female = 79, 15–17 years = 62, 18–24 years = 48) were included in analysis; 55.5% (n= 61) reporting dissatisfaction with their sleep and 62.7% (n= 69) reporting typically less than 7 hr sleep per night. Sleep duration was significantly shorter for those who reported 1–2 or ≥3 awakenings to provide care, compared with no awakenings (p_< .05). Sleep quality, as described by scores on the Pittsburgh Sleep Quality Index (PSQI) was also significantly worse for those who were frequently awoken by their care recipient (p < .05). Worrying about the care recipient, being woken by the care recipient, and listening out for the care recipient were the most frequently identified factors impacting on sleep. Conclusion Young carers experience reduced sleep duration and poor sleep quality. Strategies to alleviate the burden of care work on young carer’s sleep would benefit the health and safety of this group.
The landscape of submicroscopic structural variants at the OPN1LW/OPN1MW gene cluster on Xq28 underlying blue cone monochromacy

Significance Blue cone monochromacy (BCM) is an inherited retinal disorder characterized by low vision and poor color vision and caused by mutations in the multicopy gene cluster encoding the long- and middle-wavelength-sensitive cone photoreceptor visual pigments. We showed that structural genomic mutations at the gene cluster explain about one-third of those affected among 213 genetically confirmed BCM families. Our study expands the known spectrum of structural mutations causing BCM by a factor of 4 and provides a comprehensive landscape of their extent and fine structure as well as a deep insight into the underlying molecular mechanisms. We observed evidence that occurrence of BCM-linked structural mutations may be driven by inherent increased instability of individual gene clusters with large copy numbers.
COVID-19 vaccination rates in hospitalized mentally ill patients compared to the general population in Germany: Results from the COVID Ψ Vac study

Abstract Background Mental illness is known to come along with a large mortality gap compared to thegeneral population and it is a risk for COVID-19 related morbidity andmortality. Achieving high vaccination rates in people with mental illness is therefore important. Reports are conflicting on whether vaccination rates comparable to those of the general population can be achieved and which variables represent risk factors for nonvaccination in people with mental illness. Methods The COVID Ψ Vac study collected routine data on vaccination status, diagnostic groups, sociodemographics, and setting characteristics from in- and day-clinic patients of 10 psychiatric hospitals in Germany in August 2021. Logistic regression modeling was used to determine risk factors for nonvaccination. Results Complete vaccination rates were 59% (n = 776) for the hospitalized patients with mental illness versus 64% for the regionally and age-matched general population. Partial vaccination rates were 68% (n = 893) for the hospitalised patients with mental illness versus 67% for the respective general population and six percentage (n = 74) of this hospitalized population were vaccinated during the hospital stay. Rates showed a large variation between hospital sites. An ICD-10 group F1, F2, or F4 main diagnosis, younger age, and coercive accommodation were further risk factors for nonvaccination in the model. Conclusions Vaccination rates were lower in hospitalized people with mental illness than in the general population. By targeting at-risk groups with low-threshold vaccination programs in all health institutions they get in contact with, vaccination rates comparable to those in the general population can be achieved.
Technology-Based Responses to Technology-Facilitated Domestic and Family Violence: An Overview of the Limits and Possibilities of Tech-Based “Solutions”

It is increasingly common for domestic and family violence to have an element of technology-facilitated abuse (TFA). As a result, technology-based responses have emerged to address TFA. Using observations from several empirical research projects into TFA, it will be shown that technology-based responses are necessary without being sufficient, and that they have persistent limitations that need to be recognized. Relatedly, it will be argued that there should be an ongoing emphasis on the development of human resources as a support for those experiencing TFA, particularly the use of professional DV support workers.
Monitoring Pulmonary Thrombectomy: What Information Can Be Gained with Arterial Spin Labeling MRI?

Copyright © 2022 The Korean Society of Radiology Monitoring Pulmonary Thrombectomy: What Information Can Be Gained with Arterial Spin Labeling MRI? Cecilia Zhang, Gerd Groezinger, Klaus-Peter Kreißelmeier, Ahmed E. Othman, Petros Martirosian, Rolf Pohmann, Ferdinand Seith Departements of Diagnostic and Interventional Radiology and Cardiology and Angiology, University of Tübingen, Tübingen, Germany; Section on Experimental Radiology, University of Tübingen, Tübingen, Germany; High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany