Miracle-dz/UniFAR-FTUs huggingface dataset:
Low-dose mesenchymal stem cell therapy for discogenic pain: safety and efficacy results from a 1-year feasibility study

Aim: To evaluate safety and efficacy of low dose autologous adipose-derived mesenchymal stem cells (ADMSCs) for treatment of disc degeneration resulting in low back pain (LBP). Methods: Nine participants with chronic LBP originating from single-level lumbar disc disease underwent intradiscal injection of 10 million ADMSCs with optional repetition after 6 months. Results: No unexpected or serious adverse events were recorded. Seven (78%) of participants reported reductions in pain 12 months after treatment. Five (56%) reported increased work capacity. Three (33%) reduced analgesic medication. Improvements in EQ-5D and Oswestry disability index results were observed. MRI demonstrated no further disc degeneration and improvements to annular fissures and disc protrusions. Conclusion: This study provides initial evidence of safety and efficacy of ADMSCs for discogenic LBP.
Integrating Genome-Wide Association Study with Transcriptomic Analysis to Predict Candidate Genes Controlling Storage Root Flesh Color in Sweet Potato

Sweet potato is a hexaploid heterozygote with a complex genetic background, self-pollination infertility, and cross incompatibility, which makes genetic linkage analysis quite difficult. Genome-wide association studies (GWAS) provide a new strategy for gene mapping and cloning in sweet potato. Storage root flesh color (SRFC) is an important sensory evaluation, which correlates with storage root flesh composition, such as starch, anthocyanin, and carotenoid. We performed GWAS using SRFC data of 300 accessions and 567,828 single nucleotide polymorphism (SNP) markers. Furthermore, we analyzed transcriptome data of different SRFC varieties, and conducted real-time quantitative PCR (qRT-PCR) to measure the expression level of the candidate gene in purple and non-purple fleshed sweet potato genotypes. The results showed that five unique SNPs were significantly (−log10P > 7) associated with SRFC. Based on these trait-associated SNPs, four candidate genes, g55964 (IbF3′H), g17506 (IbBAG2-like), g25206 (IbUGT-73D1-like), and g58377 (IbVQ25-isoform X2) were identified. Expression profiles derived from transcriptome data and qRT-PCR analyses showed that the expression of g55964 in purple-fleshed sweet potato was significantly (p < 0.01) higher than that of non-purple fleshed sweet potato. By combining the GWAS, transcriptomic analysis and qRT-PCR, we inferred that g55964 is the key gene related to purple formation of storage root in sweet potato. Our results lay the foundation for accelerating sweet potato genetic improvement of anthocyanin through marker-assisted selection.
Drivers of inorganic carbon dynamics and air–water CO2 fluxes in two large tropical estuaries: Insights from coupled radon (222Rn) and pCO2 surveys

Estuaries and coastal waters are generally significant emitters of CO2 to the atmosphere. Globally, submarine groundwater discharge (SGD) is a significant driver of inorganic carbon dynamics and the partial pressure of CO2 in water [pCO2(water)] in estuaries and coastal waters. However, there are few studies of CO2 emission and SGD in large tropical estuaries. To investigate the drivers of pCO2(water) dynamics in two large tropical estuaries in India, the Hooghly and Matla estuaries, we conducted coupled radon and pCO2(water) surveys of groundwater and surface waters. We also collected high‐temporal‐resolution (1 min) data of related biogeochemical parameters. Using radon‐222 (222Rn) as a proxy for SGD, we found significant regulation of pCO2(water) by SGD in both studied estuaries. This observation can be considered direct evidence of the influence of SGD on pCO2(water) and consequent air–water CO2 fluxes in these two previously poorly studied estuaries. Our finding that total alkalinity (TAlk) and dissolved inorganic carbon (DIC) were also significantly higher in the groundwater than in the estuarine surface waters also supports a substantial contribution of SGD to the inorganic carbon dynamics of these estuaries. However, high groundwater TAlk to DIC ratios (> 1) indicate that SGD also enhances the carbonate buffering capacity in these estuaries. By contributing to an improved understanding of the role of SGD in inorganic carbon cycling in tropical estuaries and coastal oceans, this study will contribute to efforts to upscale regional‐scale coastal carbon budgets to a global scale.
Rethinking civic education in the digital era: How media, school, and youth negotiate the meaning of citizenship

This study clarifies the evolving notion of civic education that resulted from technological advancement. We investigated Singaporean young people's views on civic education by contrasting the roles of schools, families and friends, and social media in shaping their understanding. Through focus groups, we found that there are contentions on the definitions, approaches, and meanings of civic education. We conclude that in view of the inevitable diversity digital technologies tend to present, young people's civic learning might be limited by the pressure and confusion coming along with the exposure to diverse views, the tendency to retreat from formal politics, and the lack of effective school intervention into the social media space.
Polychronicity, Time Perspective, and Procrastination Behavior at the Workplace: An Empirical Study

Daily duties, tasks, various situations, and decisions must be resolved. However, it is frequently the case that we defer critical activities till later due to an overload of information from many sources, an excessive number of duties, the quick pace of life, and so on. Procrastination behavior impedes the development of every organization. The main objective is to identify possible differences in procrastination and the proper use of polychronicity in a cross-cultural context. A sample of 281 employees of manufacturing industries in Pakistan was considered. A study proposed hypotheses were tested through Structural Equation Modeling (SEM) technique. The findings indicated that polychronicity increased procrastinating behavior, the role of conflict mediation influence. Furthermore, job autonomy acts as a negative moderator of polychronicity influence on the role of conflict occurrences, therefore weakening the entire mediating process between polychronicity and individual procrastinating behaviour. Finally, the results are used to show the model's theoretical and practical implications. Deben resolverse los deberes diarios, las tareas, las diversas situaciones y las decisiones. Sin embargo, con frecuencia postergamos las actividades críticas para más tarde debido a una sobrecarga de información de diversas fuentes, un número excesivo de tareas, el ritmo de vida rápido, etc. El comportamiento de procrastinación impide el desarrollo de todas las organizaciones. El objetivo principal es identificar las posibles diferencias en la procrastinación y el uso adecuado de la policronicidad en un contexto transcultural. Se consideró una muestra de 281 empleados de industrias manufactureras en Pakistán. Las hipótesis propuestas en este estudio fueron probadas mediante la técnica de Modelado de Ecuaciones Estructurales (SEM). Los hallazgos indicaron que la policronicidad aumentó el comportamiento de procrastinación, el papel de la influencia de la mediación de conflictos. Además, la autonomía laboral actúa como un moderador negativo de la influencia de la policronicidad en el papel de los sucesos de conflicto, debilitando así todo el proceso de mediación entre la policronicidad y la conducta procrastinadora individual. Finalmente, los resultados se utilizan para mostrar las implicaciones teóricas y prácticas del modelo.
Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis

Ischemic stroke is a major condition that remains extremely problematic to treat. A cerebral reperfusion injury becomes apparent after an ischemic accident when reoxygenation of the afflicted area produces pathological side effects that are different than those induced by the initial oxygen and nutrient deprivation insult. Pyroptosis is a form of lytic programmed cell death that is distinct from apoptosis, which is initiated by inflammasomes and depends on the activation of Caspase-1. Then, Caspase-1 mobilizes the N-domain of gasdermin D (GSDMD), resulting in the release of cytokines, such as interleukin-1β (IL-1β) and interleukin-18 (IL-18). X-box binding protein l (XBP-1) is activated under endoplasmic reticulum (ER) stress to form an important transcription factor XBP-1 splicing (XBP-1s). The cerebral ischemia/reperfusion (CI/R) causes cytotoxicity, which correlates with the activation of splicing XBP-1 mRNA and NLRP3 (NOD-, LRR-, and pyrin domain-containing 3) inflammasomes, along with increases in the expression and secretion of proinflammatory cytokines and upregulation of pyroptosis-related genes in HT22 cells and in the middle cerebral artery occlusion (MCAO) rat model. However, whether XBP-1 plays a role in regulating pyroptosis involved in CI/R is still unknown. Our present study showed that behavior deficits, cerebral ischemic lesions, and neuronal death resulted from CI/R. CI/R increased the mRNA level of XBP-1s, NLRP3, IL-1β, and IL-18 and the expressions of XBP-1s, NLRP3, Caspase-1, GSDMD-N, IL-1β, and IL-18. We further repeated this process in HT22 cells and C8-B4 cells and found that OGD/R decreased cell viability and increased LDH release, XBP-1s, NLRP3, Caspase-1, GSDMD-N, IL-1β, IL-18, and especially the ratio of pyroptosis, which were reversed by Z-YVAD-FMK and downregulated XBP-1. Our results suggest that downregulated XBP-1 inhibited pyroptosis through the classical NLRP3/Caspase-1/GSDMD pathway to protect the neurons.
Rekonstrukce 3D modelu prostředí helikoptérou

Pokyny pro vypracování: 1. Prostudujte princip měření vzdálenosti kamerovým stereo-párem a vhodné metody současné lokalizace a mapování (SLAM) pro registraci jednotlivých měření a vytvoření 3D mapy. 2. Seznamte se s použitím kamery ZED. 3. Navrhněte a implementujte metodu vhodnou pro použití na malé bezpilotní helikoptéře. 4. Proveďte experimenty v reálném prostředí a vyhodnoťte použitelnost metody pro helikoptéru, její přesnost, robustnost a omezení.
Housing and Environmental Enrichment of the Domestic Ferret: A Multi-Sector Survey

Simple Summary How ferrets across sectors are housed and the environmental enrichment provided (e.g., toys, beds, exploration of new sights and smells) can directly impact their health and wellbeing. Through an online questionnaire reaching ferret caretakers from pet owner, laboratory, zoo, rescue, and working (e.g., pest control) sectors, we describe how ferrets are housed, the enrichment they receive, enrichment types that ferrets most enjoy and those which may be harmful or problematic. Of 754 responses, 82.4% were from pet owners. Most ferrets were housed with at least one other ferret, and the type of housing varied across sectors from single-level cages to free-ranging housing. Environmental enrichments most commonly reported were hammocks, tunnels and human interaction, with ferrets reported to most enjoy digging, tunnels, human interaction and exploration. Scent trails were also reported to be among the most enjoyable enrichments but were rarely provided, suggesting that they could be used more. Problematic enrichment included rubber items, such as Kongs®, which could be chewed and swallowed, narrow tunnels trapping ferrets, and fabrics catching claws. These items should therefore be avoided. Our results suggest that all sectors have room to improve both housing and enrichment to better ferret welfare. Abstract Ferrets (Mustela putorius furo) are kept and used in multiple sectors of society, but little is known about how they are housed and what environmental enrichment (EE) they may benefit from. We aimed to help guide caretakers about what housing and EE can be provided for ferrets. Through an online questionnaire of ferret caretakers, including pet, laboratory, zoological collection, rescue and working animal sectors internationally, we described ferret housing, opportunities for exploration, EE provision and caretaker opinions on ferrets’ preferred EE types, and problematic EE. In total, 754 valid responses from 17 countries were analysed, with most (82.4%) coming from pet owners. Most ferrets were housed socially, with housing varying across sectors from single-level cages to free-range housing in a room or outdoor enclosure; pet owners mostly used multi-level cages. The most commonly reported EE included hammocks, tunnels and tactile interaction with caretakers. Respondents reported that ferrets particularly enjoyed digging substrates, tunnels, human interaction and exploration. The most frequently reported problems were that ingestion of unsuitable chew toys and rubber items could cause internal blockages, narrow tunnels could trap ferrets, and certain fabrics that could catch claws. This suggests a need for increased awareness of the risks of these EE types and for more commercially available safety-tested ferret EE. Scent trails were relatively rarely provided but were reported to be enjoyed and harmless, so we recommend that these should be provided more commonly. Our results suggest that there is scope to improve ferret housing and EE provision to benefit ferret welfare across all sectors.
Wrinkle-structured MXene film assists flexible pressure sensors with superhigh sensitivity and ultrawide detection range

Abstract With the rapid development of artificial intelligence and ever-increasing demand for electronic devices, constructing high-performance flexible pressure sensors is urgently needed. In this study, a novel flexible pressure sensor with superhigh sensitivity and ultrawide detection range is tactfully designed and prepared based on wrinkle-structured MXene film. Due to the unique wrinkle structure, the prepared pressure sensor exhibits outstanding performance including high sensitivity of nearly 860 kPa−1, wide detection range (0.5 Pa ‒ 30 kPa), short response/recover time (40/53 ms) and high stability (11600 cycles). A multi-scale working mechanism is proposed to explain the sensing process. Interestingly, the prepared sensor can easily detect the smallest movement of a millet (2.4 mg) in real time. Based on these advantages, the prepared pressure sensor is expected to show significant potentials in future physiological detection and precision instrument monitoring systems. Graphical Abstract
Role of salivary glycopatterns for oral microbiota associated with gastric cancer.

Microbiota in the oral cavity plays an important role in maintaining human health. Our previous studies have revealed significant alterations of salivary glycopatterns in gastric cancer (GC) patients, but it is unclear whether these altered salivary glycopatterns can cause the dysbiosis of oral microbiota. In this study, the oral microbiome of healthy volunteers (HVs) and GC patients were detected. The neoglycoproteins were then synthesized according to the altered glycopatterns in GC patients and used to explore the effects of specific salivary glycopattern against oral microbiota. The results showed that five species were significantly increased (p < 0.05) while two species were significantly decreased (p < 0.01) in the saliva of GC patients compared with that of HVs. And the fucose-neoglycoproteins (30-100 μg/mL) could reduce the adhesion and toxicity of Aggregatibacter segnis (A. segnis) to oral cells (HOEC and CAL-27), change the glycan structures of lipopolysaccharide on the surface of A. segnis, and enhance the capacity of A. segnis to trigger innate immune responses. This study revealed that the changes of salivary protein glycopatterns in GC patients might contribute to the dysbiosis of oral microbiota, and had important implications in developing new carbohydrate drugs to maintain a balanced microbiota in the oral.
Building and Using Multiple Stacks of Models for the Classification of Learners and Custom Recommending of Quizzes

Recommending quizzes in e-Learning systems always represents a challenging task, as the quality of recommendations may have a high impact on the student’s progress. We propose a data analysis workflow based on building multiple stacks of models that use information from former students’ taken quizzes. The current implementation uses the RandomForest algorithm for building the models on a real-world dataset that has been obtained in a controlled environment. As preprocessing techniques, we have used normalization and discretization such that training data have been used for classification and regression tasks. At run-time, the models are queried for classifying the student and inferring an optimal quiz that is personalized for the student. We have evaluated the accuracy parametrized on the previous number of quizzes and found that a possible optimal timeframe for each class of students should be used and may provide more helpful quizzes.
Numerical prediction of cavitation in noncircular journal bearings

A numerical investigation of gaseous cavitation in noncircular journal bearings of finite length is presented in this paper. Four bearing profiles are considered: circular, worn-circular, two-lobe (lemon) and elliptical. The alternating direction implicit (ADI) method is used to numerically solve a single partial differential equation that is valid throughout the bearing, i.e., both in full film and cavitated regions. The equation is part of the Elrod algorithm which is used to simulate bearing cavitation. This procedure, which conserves mass throughout the bearing, automatically implements boundary conditions at rupture and reformation that are thought to model moderately to heavily loaded bearings. Performance predictions are compared to results obtained from a variety of earlier papers. Presented at the 43rd Annual Meeting in Cleveland, Ohio May 9–12, 1988
DEVELOPMENT OF AN INSPECTION PLATFORM AND A SUITE OF SENSORS FOR ASSESSING CORROSION AND MECHANICAL DAMAGE ON UNPIGGABLE TRANSMISSION MAINS

The National Energy Technology Laboratory of the US Department of Energy (under Award DE-FC26-02NT41645) and the NYSEARCH Committee of the Northeast Gas Association (previous the New York Gas Group), have sponsored research to develop a robotic pipeline inspection system capable of navigation through the typical physical and operational obstacles that make transmission and distribution pipelines unpiggable. The research contractors, Foster-Miller and GE Oil & Gas (PII North America) have performed an engineering study and developed a conceptual design that meets all the requirements for navigating and inspecting unpiggable transmission pipelines. Based on Foster-Miller's previous efforts developing the Pipe Mouse robot, the RoboScan inspection robot (Figure ES-1) meets the navigational and physical challenges of unpiggable pipelines through an innovative modular platform design, segmented MFL inspection modules and improvements to the inter-module coupling design.
Distributed output‐feedback fault detection and isolation of cascade process networks

Distributed output-feedback fault detection and isolation (FDI) of nonlinear cascade process networks that can be divided into subsystems is considered. Based on the assumption that an exponentially convergent estimator exists for each subsystem, a distributed state estimation system is developed. In the distributed state estimation system, a compensator is designed for each subsystem to compensate for subsystem interaction and the estimators for subsystems communicate to exchange information. It is shown that when there is no fault, the estimation error of the distributed estimation system converges to zero in the absence of system disturbances and measurement noise. For each subsystem, a state predictor is also designed to provide subsystem state predictions. A residual generator is designed for each subsystem based on subsystem state estimates given by the distributed state estimation system and subsystem state predictions given by the predictor. A subsystem residual generator generates two residual sequences, which act as references for FDI. A distributed FDI mechanism is proposed based on residuals. The proposed approach is able to handle both actuator faults and sensor faults by evaluating the residual signals. A chemical process example is introduced to demonstrate the effectiveness of the distributed FDI mechanism. © 2017 American Institute of Chemical Engineers AIChE J, 63: 4329–4342, 2017
Influence of anthropometric, ball impact and landing location parameters on serve velocity in elite tennis competition

This study aimed (i) to analyse the associations between serve velocity (SV) and anthropometric, ball impact and landing location parameters in total serves (TS) and fastest serves (FS) performed during an ATP Tour event; (ii) to observe differences between first (S1) and second (S2) serves, and (iii) to determine a SV prediction model based on the relationship between the observed variables. Using Foxtenn technology, 30 S1 and 15 S2 were registered in 14 matches in twenty-one male professional tennis players. Ball impact (impact height [IH], impact projection angle [IPA] and relative impact height [RIH]), bounce landing (width and depth) location parameters, S1 and S2 SV in TS (TSV1 and TSV2) and FS (FSV1 and FSV2) alongside anthropometric characteristics of tennis players (body height [BH], body mass [BM] and body mass index [BMI]) were analysed. Significant moderate to large associations were found between BH and BM and TSV1, FSV1 and FSV2 (r = 0.315 to 0.593; p < 0.001), and between IH and IPA and TSV1 and TSV2 (r = 0.294 to -0.409; p < 0.001). BH and BM were the unique significant contributors of FS explaining 22 to 35% of FSV1 and FSV2. Only BM appears in the model to predict FSV1 and FSV2 (r2 = 0.48 and 0.21). We concluded that all three anthropometric, ball impact and bounce landing location parameters small to moderately influence TSV. Anthropometric parameters show an impact on SV when tennis players serve at or near maximal speed, highlighting the influence of BM above BH.
Binding of GS-461203 and Its Halogen Derivatives to HCV Genotype 2a RNA Polymerase Drug Resistance Mutants

Hepatitis C Virus (HCV) is reported to develop GS-461203 resistance because of multiple mutations within the RNA-dependent RNA Polymerase (RdRp) of HCV. The lack of a high-resolution structure of these RdRp mutants in complex with GS-461203 hinders efforts to understand the drug resistance. Here we decipher the binding differences of GS-461203 in the wild type and mutated systems T179A or M289L of HCV RdRp Genotype 2a using homology modeling, molecular docking, and molecular dynamics simulation. Key residues responsible for GS-461203 binding were identified to be Arg48, Arg158, Asp318, Asp319, and Asp220, and that mutations T179A or M289L have caused conformational changes of GS-461203 in the RdRp active site. The affinities of GS-461203 were reduced in T179A system, but it became slightly stronger in the M289L system. Furthermore, we designed two new analogues of GS-461203 which encouragingly induced more stable interactions than GS-461203, and thus resulted in much better binding energies. This present study reveals how a single mutation, T179A or M289L, will modulate GS-461203 binding in HCV RdRp Genotype 2a, while introducing two novel analogues to overcome the drug resistance which may be good candidate for further experimental verification.
Adaptability Improvement of Learning from Demonstration with Particle Swarm Optimization for Motion Planning

We present a method for improving adaptability of Learning from Demonstration (LfD) strategy by combining the LfD and Particle Swarm Optimization (PSO). A trajectory generated from an LfD is modified with PSO by minimizing a fitness function that considers constraints. Finally, the final trajectory is suitable for a task and adapted for constraints. The effectiveness of the method is shown with a target reaching task with a manipulator in three-dimensional space.