Miracle-dz/UniFAR-FTUs huggingface dataset:
Correction to: 2019 ACC/AHA Guideline on the Primary Prevention of Cardiovascular Disease: A Report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines.

Author(s): Arnett, Donna K; Blumenthal, Roger S; Albert, Michelle A; Buroker, Andrew B; Goldberger, Zachary D; Hahn, Ellen J; Himmelfarb, Cheryl Dennison; Khera, Amit; Lloyd-Jones, Donald; McEvoy, J William; Michos, Erin D; Miedema, Michael D; Munoz, Daniel; Jr, Smith Sidney C; Virani, Salim S; Sr, Williams Kim A; Yeboah, Joseph; Ziaeian, Boback
Genome‐wide association study of circulating folate one‐carbon metabolites

Experimental, observational, and clinical trials support a critical role of folate one‐carbon metabolism (FOCM) in colorectal cancer (CRC) development. In this report, we focus on understanding the relationship between common genetic variants and metabolites of FOCM. We conducted a genome‐wide association study of FOCM biomarkers among 1,788 unaffected (without CRC) individuals of European ancestry from the Colon Cancer Family Registry. Twelve metabolites, including 5‐methyltetrahydrofolate, vitamin B2 (flavin mononucleotide and riboflavin), vitamin B6 (4‐pyridoxic acid, pyridoxal, and pyridoxamine), total homocysteine, methionine, S‐adenosylmethionine, S‐adenosylhomocysteine, cystathionine, and creatinine were measured from plasma using liquid chromatography‐mass spectrometry (LC‐MS) or LC‐MS/MS. For each individual biomarker, we estimated genotype array‐specific associations followed by a fixed‐effect meta‐analysis. We identified the variant rs35976024 (at 2p11.2 and intronic of ATOH8) associated with total homocysteine (p = 4.9 × 10−8). We found a group of six highly correlated variants on chromosome 15q14 associated with cystathionine (all p < 5 × 10−8), with the most significant variant rs28391580 (p = 2.8 × 10−8). Two variants (rs139435405 and rs149119426) on chromosome 14q13 showed significant (p < 5 × 10−8) associations with S‐adenosylhomocysteine. These three biomarkers with significant associations are closely involved in homocysteine metabolism. Furthermore, when assessing the principal components (PCs) derived from seven individual biomarkers, we identified the variant rs12665366 (at 6p25.3 and intronic of EXOC2) associated with the first PC (p = 2.3 × 10−8). Our data suggest that common genetic variants may play an important role in FOCM, particularly in homocysteine metabolism.
Multi-method assessment of whale shark (Rhincodon typus) residency, distribution, and dispersal behavior at an aggregation site in the Red Sea

Whale sharks (Rhincodon typus) are typically dispersed throughout their circumtropical range, but the species is also known to aggregate in specific coastal areas. Accurate site descriptions associated with these aggregations are essential for the conservation of R. typus, an Endangered species. Although aggregations have become valuable hubs for research, most site descriptions rely heavily on sightings data. In the present study, visual census, passive acoustic monitoring, and long range satellite telemetry were combined to track the movements of R. typus from Shib Habil, a reef-associated aggregation site in the Red Sea. An array of 63 receiver stations was used to record the presence of 84 acoustically tagged sharks (35 females, 37 males, 12 undetermined) from April 2010 to May 2016. Over the same period, identification photos were taken for 76 of these tagged individuals and 38 were fitted with satellite transmitters. In total of 37,461 acoustic detections, 210 visual encounters, and 33 satellite tracks were analyzed to describe the sharks’ movement ecology. The results demonstrate that the aggregation is seasonal, mostly concentrated on the exposed side of Shib Habil, and seems to attract sharks of both sexes in roughly equal numbers. The combined methodologies also tracked 15 interannual homing-migrations, demonstrating that many sharks leave the area before returning in later years. When compared to acoustic studies from other aggregations, these results demonstrate that R. typus exhibits diverse, site-specific ecologies across its range. Sightings-independent data from acoustic telemetry and other sources are an effective means of validating more common visual surveys.
Otitis media pathogens – A life entrapped in biofilm communities

Abstract Otitis media is a group of inflammatory diseases of the middle ear with great impact on children worldwide. The most common reported bacterial pathogens are Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis. Over the last years, the role of biofilms formed by otopathogens that contribute to otitis media recurrence and chronicity has been established. An improved understanding of the properties of biofilms formed by these bacteria, which factors influence them, and how these affect the host inflammatory response is important for the development of novel strategies for the treatment of otitis media. This review focuses on the biofilm nature that the most prevalent otopathogens adopt in otitis media infections. In addition, new treatment approaches targeting biofilms are highlighted.
A six‐CpG‐based methylation markers for the diagnosis of ovarian cancer in blood

DNA methylation markers in the peripheral blood are able to be applied to treat epithelial cancer. Nevertheless, the diagnostic potential value of it for ovarian cancer (OV) has not been studied. The study aimed to explore the difference of DNA methylation in peripheral blood between OV patients and healthy women. Firstly, the whole blood of DNA methylation data was provided by the Gene Expression Omnibus (GEO) database. The linear model was applied to the identification of significantly differentially expressed methylated CpG sites (differentially methylation sites [DMP]), and the further screen of co‐expression CpG sites (Co‐DMP). A total of 2812 DMPs were identified, and weighted gene co‐expression network analysis helped to obtain seven co‐expression modules. Among them, the yellow module was the most related to OV. Co‐DMPs (167) in the yellow module were mainly distributed near the transcription start sites. However, most of them were not in the CpG island. Least absolute shrinkage and selection operator (LASSO) regression analysis was applied to the identification of stable OV‐related blood biomarkers that six Co‐DMPs (cg00134539, cg00226923, cg25268718, cg25697314, cg25839227, cg26574610) with the highest frequency were found as potential biomarkers. Finally, the diagnostic classifier was established using the support vector machine (SVM) with the accuracy rate of 87.1% and 74.5% in training data set and validation data set, respectively. To sum up, a new feature was provided here for the diagnosis of OV, which is helpful for the diagnosis and individualized treatments of early OV.
Salvage pelvic lymph node dissection for lymph node recurrent prostate cancer.

PURPOSE OF REVIEW The aim of this article is to update on the role of salvage pelvic lymph node dissection (SLND) for patients with lymph node-only recurrent prostate cancer (PCa) after radical prostatectomy. RECENT FINDINGS Salvage lymph-node dissection represents a possible treatment option for selected patients with lymph-node only recurrent PCa detected by PET/CT. Although strong prospective evidence supporting its role is still missing, SLND is associated with encouraging oncological outcomes with 2-year biochemical progression-free survival ranging from 23 to 64%, and 5-year overall survival of 84%. However, the best oncological outcomes after SLND can only be reached with accurate patient election based on imaging and clinical features at the time of SLND. Finally, the use of the robotic approach for SLND is increasing, being associated with similar oncological outcomes but lower perioperative complication rates as compared with the open approach. SUMMARY SLND represents a possible treatment option in lymph node only recurrent PCa, provided careful patient selection. It is likely that the introduction of novel imaging tracers and the use of a robotic approach will favourably impact on perioperative and oncological outcomes. Nevertheless, future randomized studies are awaited to confirm the effectiveness and utility of this experimental approach.
Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.
Improved Quality of Life Following Addiction Treatment Is Associated with Reductions in Substance Use

People seeking treatment for substance use disorders (SUD) ultimately aspire to improve their quality of life (QOL) through reducing or ceasing their substance use, however the association between these treatment outcomes has received scant research attention. In a prospective, multi-site treatment outcome study (‘Patient Pathways’), we recruited 796 clients within one month of intake from 21 publicly funded addiction treatment services in two Australian states, 555 (70%) of whom were followed-up 12 months later. We measured QOL at baseline and follow-up using the WHOQOL-BREF (physical, psychological, social and environmental domains) and determined rates of “SUD treatment success” (past-month abstinence or a statistically reliable reduction in substance use) at follow-up. Mixed effects linear regression analyses indicated that people who achieved SUD treatment success also achieved significantly greater improvements in QOL, relative to treatment non-responders (all four domains p < 0.001). Paired t-tests indicated that non-responders significantly improved their social (p = 0.007) and environmental (p = 0.033) QOL; however, their psychological (p = 0.088) and physical (p = 0.841) QOL did not significantly improve. The findings indicate that following treatment, QOL improved in at least some domains, but that reduced substance use was associated with both stronger and broader improvements in QOL. Addressing physical and psychological co-morbidities during treatment may facilitate reductions in substance use.
Study of blood cultures by BACTEC method in pediatric patients of Chhattisgarh, India

Background: 356 children aged between 2 to 4 years admitted at PICU were selected for study B.D BACTEC 9050 system was used for incubation bottle were incubated until microbial growth was detected, BACTEC 9050 is an automated blood culture system which responds to concentration of Co2 produced by metabolism microorganism or consumption of O2, needed for growth of microorganism.Methods: The antibiotic used were Amoxicin 250 mcg, penicillin 100 unit linezoid 50 Mcg, vancomycin 50 mcg, ampicillin 100 mcg, Azithromycin 100 Mcg, pipericillin/ Tozabactum, 100/50mcg, ceforazone/ salbactum 50/30 mcg, cefoxitin 50 mcg, cefepime 50 mcg, Amikacin 50 mcg, Imipenen 30 mcg, ceftazidime- clavulanic acid.Results: The observed organized were 90 (25.2%) CoNS, 72 (20.2%) Entrobacter, 70 (19.6%) Klebesiella spp, 40(11.1%) Enterococcus, 22(6.1%) S, auresis, 22 (6.1%) E coli, 20(5.6%) citrobacters, 20(5.6%) Acinitobacter. This blood culture study by BACTEC 9050 in pediatric patients in few minutes.Conclusions: BACTEC-9050 method was helpful to treat especially in children because most of diseases in children are idiopathic and life threatening.
Automated Driving System Collisions: Early Lessons

Objective This research evaluated Automated Driving Systems (ADSs) involved collisions to identify factors relevant to future ADS research and development. Background Rapidly developing ADSs promise improved safety, among other benefits. Properly applied collision research can inform ADS development, to minimize future collisions. Errors and failures that result in collisions come from sources including the system, ADS operators, and external factors including other drivers. Partially automated systems incorporate new equipment and procedures creating new sources of human error. Fully autonomous systems represent a new class of drivers that interact in unique ways. Method ADS collision reports from the California Department of Motor Vehicles and the National Transportation Safety Board were collected. An expert in human factors and collision investigation analyzed and categorized the crashes while extracting common factors. Results ADS vehicles were never at fault but were often affected from the rear during braking, turning, and gap acceptance maneuvers. Side impacts to ADS vehicles were related to passing vehicles and lane keeping behaviors. Unique incidents also provided additional insights. ADS collision rates cannot yet be determined with confidence. Conclusion Conflicts that lead to collision-involvement with ADSs may be caused by differences between ADS and human driving behavior. Conservative ADS behavior may violate the expectations of other nearby human road users. Application The findings from this work help inform the future development of ADS, as well as potentially the testing of ADS and the formation of policy to guide their future deployment.
A Unified Design of Massive Access for Cellular Internet of Things

With the increasing development of the cellular Internet of Things (IoT), the upcoming fifth-generation wireless network is required to support massive access of sporadic traffic devices. In this context, we design a three-phase transmission protocol which consists of device detection and channel estimation, uplink data transmission, and downlink data transmission for the cellular IoT, so as to realize massive access over limited radio spectrum. We analyze the performance of the proposed transmission protocol and derive closed-form expressions for the uplink and downlink achievable rates in terms of channel conditions and system parameters. Moreover, to improve the overall performance, we propose a length allocation algorithm by coordinating the three-phase transmission protocol in the unified sense. Extensive simulation results show that substantial performance gain can be obtained by the proposed algorithm.
Real Talk: Toward Further Understanding of Case Story in Teaching Educational Administration.

A study investigated the effectiveness of an innovative approach to the case study in teaching educational administrators in master's and doctoral programs. Subjects were 80 students in three institutions. The method uses "case stories," which differ from conventional case studies in that they are highly personal written accounts of real events that include intriguing decision points and provocative undercurrents that encourage discussion. Data for the qualitative analysis were drawn from conversations about case stories, student-written case stories, a survey, and semi-structured interviews. Based on the results, a six-stage model for writing and discussing case stories was developed. Stages include: (1) freewriting; (2) writing the case story; (3) storytelling and listening; (4) small group reflection; (5) whole group reflection; and (6) a concluding discussion about the importance of understanding and improving professional practice. Participants felt the case story method has potential to enhance professional understanding and growth. It was also determined that learning occurs in different ways (intrapersonal and interpersonal) at different stages of case story development. Three themes emerged: stories help people understand what happens to them; meaning is found not only in the story, but also in the relationship of story to teller; and group meaning-making deepens understanding. (Contains 19
“Creature” teachers “monster” mathematicians: students’ views about mathematicians and their stated attitudes to mathematics

The present study aimed to examine lower secondary students’ images of mathematics comprised of stated attitudes to and perceived needs for mathematics, and their views about mathematicians and their work. A group of 1284 lower secondary students drew a picture of mathematicians and described their drawings. The students’ drawings fell into two distinct groups: drawings where students depicted a mathematician at work, and drawings where students depicted a mathematician as a mathematics teacher. In both cases students drew both negative (“creature” or “monster”) depictions and positive (“smiley”) depictions. This article presents the data on “creature” teachers or “monster” mathematicians in relation to the students’ stated attitudes to mathematics. Trends that emerged for this sample suggested that more students who represented creature teachers disliked mathematics or had mixed feelings related to the need to learn mathematics.
Human monocyte subsets in coronary artery disease and myocardial infarction

Coronary artery disease (CAD) is a disease of inflammatory aetiology, and remains the commonest cause of death globally despite therapeutic advances. Monocytes are implicated in the pathogenesis of CAD, but also in reparative mechanisms after myocardial infarction (MI) due to subset heterogeneity. The aim of this thesis was to provide a detailed phenotypic comparison of differences between the three human monocyte subsets in CAD and after MI, with particular emphasis on CD16+ monocytes which have previously been analysed as a single population rather than two distinct subsets. Longitudinal changes were analysed following MI and relationships explored with plasma cytokines. Multiple significant novel changes in monocyte phenotype attributable to specific subsets were identified, particularly related to the CD14++CD16+CCR2+ ‘Mon2’/‘Intermediate’ subset which increased in number on day 1 after MI and appeared highly functionally active. There were significant changes in expression of a range of receptors associated with inflammation, migration and reparative processes. Significant relations to plasma cytokines and the degree of myocardial damage were observed. Most monocyte parameters predictive of left ventricular ejection fraction six weeks after MI were related to the Mon2 subset. This suggests an important role for this subset in the acute phase of MI.
Çocuk psikiyatrisi polikliniğine çeşitli nedenlerle başvurmuş, akıllı telefon bağımlılığı olan ergenlerin aldığı psikiyatrik tanılar

Amac: Akilli telefon bagimliligi, cogunlukla telefondan uzak kalamama, sik sik telefonu kontrol etme, asiri akilli telefon kullanimi nedeniyle uykusuzluk ve uyku kalitesinin bozulmasi gibi belirtilerle ortaya cikmaktadir. Bu yuzden bu calismamizda poliklinigimize cesitli nedenlerle basvurmus, akilli telefon bagimliligi olan ergenlerin aldigi psikiyatrik hastaliklari degerlendirdik. Materyal ve Metot: Cocuk psikiyatrisi poliklinigine cesitli nedenlerle basvurmus olan ergenlere cep telefonu bagimliligi olcegi kisa formu doldurtuldu. Cep telefonu bagimlilik olcegi kesme puanlarini asan 80 katilimcinin, DSM-5 tani kriterlerine gore aldigi psikiyatrik tanilar degerlendirildi. Bulgular: Calismamiza katilan hastalarin 47’si erkek, 33’u kiz idi. Erkeklerin yas ortalamasi 14,03±1,5, kizlarin yas ortalamasi 13,9 ± 1,5 idi. Kizlarin akilli telefonla gecirdigi sure gunluk ortalama 5,09±1,15 saat, cep telefonu bagimliligi olcegi puani 40,06±4,4, erkeklerin telefonla gecirdigi sure gunluk ortalama 5,21 ± 1,2 saat, cep telefonu bagimliligi olcegi puani ortalamasi 41,7 ± 4,9 idi. 80 katilimcinin 51’i dikkat eksikligi hiperaktivite bozuklugu (DEHB), 35’i anksiyete bozukluklari, 25’i depresyon, 9’u davranim bozuklugu,3’u disa atim bozukluklari, 2’si tik bozuklugu tanilarini aldilar. Erkek hastalar, DEHB(%82), anksiyete bozukluklari (%29), depresyon (%19), davranim bozukluklari (%19), disa atim bozukluklari (%6), tik bozukluklari (%4) tanilarini aldilar. Kiz hastalar ise sirasiyla, anksiyete bozukluklari (%63), depresyon (%48), DEHB (%36) tanilarini aldilar. Cinsiyete gore DEHB, anksiyete bozukluklari, depresyon ve davranim bozukluklari dagilimi acisindan istatistiksel anlamli farklilik vardir ( p < 0.05). DEHB ve davranim bozuklugu orani erkeklerde daha fazla iken, anksiyete bozukluklari ve depresyon orani kizlarda daha fazla bulundu. Sonuc: Akilli telefon bagimliligi depresyonu ve anksiyete bozuklugu olan kiz ergenlerde yuksek oranda iken, erkek ergenlerde ise DEHB’ye yuksek oranda eslik etmistir. Bu yuzden akilli telefon bagimliligi olan cocuk ve ergenlerin, psikiyatrik hastaliklar acisindan degerlendirilip gerekli tedavi ve desteginin saglanmasi gerekmektedir. Ayrica akilli telefonlarin onerilen surelerde ve gerekli durumlarda kullanilmasinin saglanmasi, cocuk ve ergenlerin hem sosyal gelisimi, hem de ruh sagligi acisindan olumlu sonuclar doguracagini dusunmekteyiz. Anahtar Kelimeler: Akilli Telefon Bagimliligi, Ergenlik, Psikiyatrik Bozukluklar, Hiperaktivite, Depresyon
Comparison of in vitro and computational experiments on the relation of inter-beat interval and duration of repolarization in a specific type of human induced pluripotent stem cell-derived cardiomyocytes

We compared a published computational model of the action potential of a specific type of human induced pluripotent stem cell -derived cardiomyocytes (hiPSC-CM) with experimental field potential data with regard to their inter-beat interval and the duration of repolarization. In particular, concomitant changes in inter-beat interval and duration of repolarization were calculated after reduction and/or augmentation of specific ion channel conductances as a surrogate for pharmacological manipulation. The observed mismatches between calculations and experimental data indicate that there is information missing about the cellular test system. Based on our results we hypothesize that, among other currents, the actual If (“funny current”) may deviate from the prediction. We show that replacement of the If formulation by alternative equations causes the model predictions to change qualitatively, however, none of the available formulations is actually achieving a satisfactory match with experimental data. We suggest a strategy to clarify whether the mismatch can be completely resolved at all using single cell models and, if yes, how this goal could be reached.