Miracle-dz/UniFAR-FTUs huggingface dataset:
Protection of Polyphenols against Glyco-Oxidative Stress: Involvement of Glyoxalase Pathway

Chronic high glucose (HG) exposure increases methylglyoxal (MGO)-derived advanced glycation end-products (AGEs) and is involved in the onset of pathological conditions, such as diabetes, atherosclerosis and chronic-degenerative diseases. Under physiologic conditions the harmful effects of MGO are contrasted by glyoxalase system that is implicated in the detoxification of Reactive Carbonyl Species (RCS) and maintain the homeostasis of the redox environment of the cell. Polyphenols are the most abundant antioxidants in the diet and present various health benefits. Aims of the study were to investigate the effects of HG-chronic exposure on glyco-oxidation and glyoxalase system in intestinal cells, using CaCo-2 cells. Moreover, we studied the effect of apple polyphenols on glyco-oxidative stress. Our data demonstrated that HG-treatment triggers glyco-oxidation stress with a significant increase in intracellular Reactive Oxygen Species (ROS), lipid peroxidation, AGEs, and increase of Glyoxalase I (GlxI) activity. On the contrary, Glyoxalase II (GlxII) activity was lower in HG-treated cells. We demonstrate that apple polyphenols exert a protective effect against oxidative stress and dicarbonyl stress. The increase of total antioxidant capacity and glutathione (GSH) levels in HG-treated cells in the presence of apple polyphenols was associated with a decrease of GlxI activity.
Viral load Reduction in SHIV-Positive Nonhuman Primates via Long-Acting Subcutaneous Tenofovir Alafenamide Fumarate Release from a Nanofluidic Implant

HIV-1 is a chronic disease managed by strictly adhering to daily antiretroviral therapy (ART). However, not all people living with HIV-1 have access to ART, and those with access may not adhere to treatment regimens increasing viral load and disease progression. Here, a subcutaneous nanofluidic implant was used as a long-acting (LA) drug delivery platform to address these issues. The device was loaded with tenofovir alafenamide (TAF) and implanted in treatment-naïve simian HIV (SHIV)-positive nonhuman primates (NHP) for a month. We monitored intracellular tenofovir-diphosphate (TFV-DP) concentration in the target cells, peripheral blood mononuclear cells (PBMC). The concentrations of TFV-DP were maintained at a median of 391.0 fmol/106 cells (IQR, 243.0 to 509.0 fmol/106 cells) for the duration of the study. Further, we achieved drug penetration into lymphatic tissues, known for persistent HIV-1 replication. Moreover, we observed a first-phase viral load decay of −1.14 ± 0.81 log10 copies/mL (95% CI, −0.30 to −2.23 log10 copies/mL), similar to −1.08 log10 copies/mL decay observed in humans. Thus, LA TAF delivered from our nanofluidic implant had similar effects as oral TAF dosing with a lower dose, with potential as a platform for LA ART.
The Impact of Telemedicine Adoption on a Multidisciplinary Bariatric Surgery Practice During the COVID-19 Pandemic

Objective: This study aims to show how full-time telemedicine adoption has impacted patient visit volume and attendance in a comprehensive metabolic and weight loss center. Summary Background Data: Elective surgical practices have been profoundly impacted by the global COVID-19 pandemic, leading to a rapid increase in the utilization of telemedicine. The abrupt initiation of audio-video telehealth visits for all providers of a multidisciplinary clinic on March 19th 2020 provided unique circumstances to assess the impact of telemedicine. Methods: Data from the clinical booking system (new patient and follow-up visits) for all clinical provider types of the multidisciplinary metabolic center from the pre-telehealth, post-telehealth, and a 2019 comparative period were retrospectively reviewed and compared. The primary outcome is the change in patient visit volume for all clinical providers from before to after the initiation of telemedicine for both new patient, and follow-up visits. Results: There were a total of 506 visits (162 new patient visits, and 344 follow-ups) in the pre-telehealth period, versus 413 visits (77 new patient visits, and 336 follow-ups) during the post-telehealth period. After telehealth implementation, new visits for surgeons decreased by 75%. Although follow-up visits decreased by 55.06% for surgeons, there was an increase by 27.36% for advanced practitioners. When surgeons were separated from other practitioners, their follow-up visit rate decrease by 55.06%, compared to a 16.08% increase for the group of all other practitioners (P < 0.0001). Dietitians experienced higher rates of absenteeism with new patient visits (10.00% vs 31.42%, P = 0.0128), whereas bariatricians experienced a decrease in follow-up visit absenteeism (33.33% vs 0%, P = 0.0093). Conclusions: Although new patient visit volume fell across the board, follow-up visits increased for certain nonsurgical providers. This provides a template for adoption of a multidisciplinary telehealth clinic in a post-pandemic world.
The Brazilian-adapted Working Alliance Inventory: preliminary report on the psychometric properties of the original and short revised versions

Abstract Introduction Alliance is an essential component of all psychotherapies and a consistent predictor of its outcomes. The Working Alliance Inventory (WAI) is a widely used and psychometrically sound measure of alliance. It assesses three key aspects of the construct: a) agreement on the tasks of therapy; b) agreement on the therapeutic goals; and c) development of an affective bond. Objective To preliminarily analyze the psychometric properties of the Brazilian version of both the original, 36-item WAI, and of the short form revised, 16-item version (WAI-SR). Methods The sample comprised 201 psychodynamic psychotherapy patients. Alliance assessments were made after the 4th treatment session. Results The inventory adapted to Brazilian Portuguese, in both the original and short forms, appears to be reliable and valid to measure alliance and its dimensions by clients in psychotherapy. Further studies are needed to replicate and expand the findings.
Strategies and Challenges to Improve Cellular Programming-Based Approaches for Heart Regeneration Therapy

Limited adult cardiac cell proliferation after cardiovascular disease, such as heart failure, hampers regeneration, resulting in a major loss of cardiomyocytes (CMs) at the site of injury. Recent studies in cellular reprogramming approaches have provided the opportunity to improve upon previous techniques used to regenerate damaged heart. Using these approaches, new CMs can be regenerated from differentiation of iPSCs (similar to embryonic stem cells), the direct reprogramming of fibroblasts [induced cardiomyocytes (iCMs)], or induced cardiac progenitors. Although these CMs have been shown to functionally repair infarcted heart, advancements in technology are still in the early stages of development in research laboratories. In this review, reprogramming-based approaches for generating CMs are briefly introduced and reviewed, and the challenges (including low efficiency, functional maturity, and safety issues) that hinder further translation of these approaches into a clinical setting are discussed. The creative and combined optimal methods to address these challenges are also summarized, with optimism that further investigation into tissue engineering, cardiac development signaling, and epigenetic mechanisms will help to establish methods that improve cell-reprogramming approaches for heart regeneration.
Does Duration of Pain at Baseline Influence Longer-Term Clinical Outcomes of Low Back Pain Patients Managed on an Evidence-Based Pathway?

STUDY DESIGN Non-randomised longitudinal observational study. OBJECTIVE To evaluate the association between baseline pain duration and medium-to-long term clinical outcomes, in low back pain (LBP) patients enrolled on the North East of England Regional Back Pain and Radicular Pain Pathway (NERBPP). SUMMARY OF BACKGROUND DATA The NERBPP is based upon National Institute for Health and Care Excellence (NICE) guidelines. These guidelines no longer differentiate management of LBP patients based on pain duration. Medium-to-long term data from the NERBPP is lacking. METHODS Between May 2015 and December 2019, 786 and 552 LBP patients from the NERBPP returned 6-month and 12-month follow-up outcome measures, respectively. Outcomes included pain (Numerical rating scale), function (Oswestry Disability Index) and quality-of-life (EuroQol five-dimension, five-level questionnaire), analysed using a series of covariate-adjusted models. Patients were categorized into four groups based upon baseline pain duration: <3 months, ≥3 to <6 months, ≥6 months to <12 months, ≥12 months. RESULTS Patients with <3 months duration demonstrated clinically important improvements on all outcomes, at both follow-ups. The improvements in outcomes from this group were larger than those in the ≥12 month's duration group (p < 0.05), these group differences in change, in some cases surpassed our threshold for clinical relevance. Functional improvements in those with ≥12 month's duration were not clinically relevant at either follow-up. All patients, regardless of baseline pain duration, reported similar levels of readiness to self-manage at the 12-month follow-up. CONCLUSIONS Baseline pain duration would appear to be of clinical importance. Patients with shorter baseline pain duration demonstrated better outcomes. Those with ≥12 month's duration of pain may need additional support during their management to achieve clinically relevant functional improvements in the medium-to-long term. These findings raise questions about the decision by NICE to move away from duration of pain to differentiate management of LBP patients. LEVEL OF EVIDENCE 3.
Combined dual energy and iterative metal artefact reduction for PET/CT in head and neck cancer

Metal artefacts in PET/CT images hamper diagnostic accuracy in head and neck cancer (HNC). The aim of this study is to characterise the clinical effects of metal artefacts on PET/CT in HNC and to inform decision-making concerning implementation of MAR techniques. We study a combined dual energy CT and inpainting-based metal artefact reduction (DECT-I-MAR) technique for PET/CT in three settings: (A) A dental phantom with a removable amalgam-filled tooth to evaluate the PET error in comparison to a known reference. (B) PET-positive patients with metallic implants to demonstrate the relationship between CT metal artefacts and PET error. (C) Metabolic tumour volumes delineated in PET-positive patients with metal implants to evaluate the clinical impact. In (A) DECT-I-MAR reduced the PET error significantly. In (B) we demonstrate an increasing PET error with increasing CT artefact severity in patients. In (C) it is shown that the presence of artefacts in the same axial slices as the tumour significantly decreases biomarker stability and increase delineation variability. This work shows the practical feasibility of DECT-I-MAR-based PET/CT imaging, and indicates a positive clinical impact of using the technique routinely for HNC patients. The impact of CT artefacts on PET is considerable, especially in workflows where quantitative PET biomarkers and tumour volumes are used. In such cases, and for patients with tumours in proximity of metals, we recommend that a MAR technique for PET/CT is employed.
Making connections: recent advances in spinal cord dorsal horn circuitry.

As the first point where sensory information enters the central nervous system, the cell types and connections in the dorsal horn are of great interest. Correct processing of sensory information within the dorsal horn relies on the balance of excitation and inhibition, although the cell types and circuitry underlying normal transmission of sensory information are still poorly understood. Despite this, there is significant evidence that changes occur in the dorsal horn in pain states, making it an attractive target for novel analgesics to stop aberrant sensory information before it reaches the brain. Progress in this area has been hindered, however, because we do not have a clear picture of how information is modulated within the dorsal horn in naive conditions, let alone in the context of injury. A number of recent studies have used genetic tools to target subpopulations of spinal neurons with the goal of understanding their function within the context of somatosensory integration. These discoveries provide important new insights, yet force us to grapple with a new of level complexity. We also need to remember that most studies to date use mouse models, and future experiments will be required to address whether these organization principles hold true in other species. In this review, we highlight some major themes that are emerging, as well as the issues that need to be addressed for a clearer understanding of the spinal processing of itch and pain, and the logic through which this information is conveyed to the brain.
Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small cell lung cancer.

MYC stimulates both metabolism and protein synthesis, but it is unknown how cells coordinate these complementary programs. Previous work reported that in a subset of small cell lung cancer (SCLC) cell lines, MYC activates guanosine triphosphate (GTP) synthesis and results in sensitivity to inhibitors of the GTP synthesis enzyme inosine monophosphate dehydrogenase (IMPDH). Here we demonstrated that primary MYCHigh human SCLC tumors also contain abundant guanosine nucleotides. We also found that elevated MYC in SCLCs with acquired chemoresistance rendered these otherwise recalcitrant tumors dependent on IMPDH. Unexpectedly, our data indicated that IMPDH links the metabolic and protein synthesis outputs of oncogenic MYC. Co-expression analysis placed IMPDH within the MYC-driven ribosome program, and GTP depletion prevented RNA Polymerase I (Pol I) from localizing to ribosomal DNA. Furthermore, the GTPases GPN1 and GPN3 were upregulated by MYC and directed Pol I to ribosomal DNA. Constitutively GTP-bound GPN1/3 mutants mitigated the effect of GTP depletion on Pol I, protecting chemoresistant SCLC cells from IMPDH inhibition. GTP therefore functions as a metabolic gate tethering MYC-dependent ribosome biogenesis to nucleotide sufficiency through GPN1 and GPN3. IMPDH dependence is a targetable vulnerability in chemoresistant, MYCHigh SCLC.
Transcriptome and Secretome Analysis of Intra-Mammalian Life-Stages of Calicophoron daubneyi Reveals Adaptation to a Unique Host Environment

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock, which has seen a rapid rise in prevalence throughout Western Europe in recent years. After ingestion of metacercariae (parasite cysts) by the mammalian host, newly excysted juveniles (NEJs) emerge and invade the duodenal submucosa, which causes significant pathology in heavy infections. The immature flukes then migrate upward, along the gastrointestinal tract, and enter the rumen where they mature and begin to produce eggs. Despite their emergence, and sporadic outbreaks of acute disease, we know little about the molecular mechanisms used by C. daubneyi to establish infection, acquire nutrients, and avoid the host immune response. Here, transcriptome analysis of four intramammalian life-cycle stages, integrated with secretome analysis of the NEJ and adult parasites (responsible for acute and chronic diseases, respectively), revealed how the expression and secretion of selected families of virulence factors and immunomodulators are regulated in accordance with fluke development and migration. Our data show that while a family of cathepsins B with varying S2 subsite residues (indicating distinct substrate specificities) is differentially secreted by NEJs and adult flukes, cathepsins L and F are secreted in low abundance by NEJs only. We found that C. daubneyi has an expanded family of aspartic peptidases, which is upregulated in adult worms, although they are under-represented in the secretome. The most abundant proteins in adult fluke secretions were helminth defense molecules that likely establish an immune environment permissive to fluke survival and/or neutralize pathogen-associated molecular patterns such as bacterial lipopolysaccharide in the microbiome-rich rumen. The distinct collection of molecules secreted by C. daubneyi allowed the development of the first coproantigen-based ELISA for paramphistomosis which, importantly, did not recognize antigens from other helminths commonly found as coinfections with rumen fluke.
Nutrient Content, in Vitro Ruminal Fermentation Characteristics and Antimethanogenic Potential of Three Algerian Asteraceae Species

The in vitro rumen fermentation parameters and the antimethanogenic potential of three Asteraceae species: Chamaemelum nobile, Centaurea pulata and Chrysanthemum segetum were determined. Serum bottles containing 200 mg of each plant and 30 ml of the culture medium (artificial saliva plus rumen juice) were incubated for 24 h. After incubation, pH, volatile fatty acid (VFA), ammonia (NH 3 ) and methane (CH 4 ) productions were recorded. Methanogens and protozoa were quantified using a Real Time PCR technique (qPCR). Cumulative gas productions, in vitro organic matter digestibility and VFA were not significantly affected by the added species when compared to the control (P > 0.05). The effects of Chamaemelum nobile and Chrysanthemum segetum on methane production, NH 3 and acetate to propionate ratio (C2:C3) were similar. The two species were able to modulate rumen fermentation to produce significantly lower CH 4 concentrations (-24.3% and -27.1%, respectively) compared to the control. C.pulata produced the highest cumulative gas and stimulated the microbial metabolism with an increase in C2:C3 ratio, NH 3 and methane production (P 0.05). The three species studied herein show a good potential for mitigating ruminal methane production without any undesirable effects on the main fermentation parameters.
Prophylactic Effects of Tomato and Lemon Juices on Castrated Healthy Local Male Rabbits

This studywas performed to determine the Prophylactic effects of both lemon and tomato juices on some physiological and biochemical features of healthy castrated male rabbits. The study included 30 male rabbits divided into 6 groups with 5 rabbits/ group; the irst group which is the control group were given physiological solution, the second were given (4ml/ kg of body weight) lemon juice, the third were given (3ml/kg of body weight) tomato juice, the fourthwere castrated andgivenphysiological solution, the ifthwere castrated and given (4ml/ kg of body weight) lemon juice and the sixth were castrated and given (3ml/kg of body weight) tomato juice. The results showed positive effects in the groups treated with the juices regarding the Adiponectin hormone, T3, T4 and TSH, as well as a reduction in concentration of TC, LDL-c, atherogenesis index and an increase in HDL-c concentration. We concluded that castrated groups was the opposite where there was a reduction in the concentrations of the Adiponectin hormone, T3, T4 and TSH, and an increase in the concentrations of TC, LDL-c, the atherogenesis index and a reduction in concentration of HDL-c.
Simulation of ATMEGA8 Microcontroller-Based Schizophrenia Patient Detector

Schizophrenia is a chronic mental disorder causing sufferers experiencing delusions, hallucinations, thoughts distracting, and behavior changing. This condition that usually lasts a long time is often interpreted as a mental disorder, given the difficulty of the patient to distinguish between reality and one's mind. The purpose of this study is to facilitate nurses when doing care, especially in the inpatient room, and so that care is more intensive . By using the magnetic sensor as a detector when the door is open, and the PIR ( Passive Infrared Receiver ) sensor as a detection of the patients flew motion, and the lamp as an indicator. The results of the data can be obtained from testing by calculating the distance if the sensor can detect. The testing results the device it is proven that the magnetic sensor can detect up to 1 cm, and the PIR sensor can detect up to 5,5 meters.
Effects of Different Combination of Growing Media on Growth and Total Phenolic Content in Labisia pumila (Kacip Fatimah)

Article history: Received 02 September 2020 Accepted 18 September 2020 Available online 05 October 2020 Labisia pumila, locally known in Malaysia as Kacip Fatimah, is highly-prized for its phenols. Phenolic compounds in L. pumila has numerous pharmacological properties such as having anti-oxidant, antimicrobial, anti-inflammatory and anti-cancer potential. The production of phenolic contents in plants is influence by various factor, including abiotic factors such as soil properties. Thus, the objective of this study was to determine the effects of different combinations of growing media on the growth and total phenolic content in L. pumila leaves. Six combinations of growing media were used, each using different types of compost that had differing amounts of L. pumila leaves. The plants were grown in all media for a period of six months at FRIM’s nursery. Besides growth performance, the total phenolic content was also determined, six months after planting. There was no significant difference in growth parameters for all treatments, with the exception of control (significantly lower than all treatments). However, the total phenolic content of Treatment 1 (80.6 mg GAE/g) was significantly higher than all other treatments. This can be attributed to the properties of Compost 1 (which was in the growth media of Treatment 1), as it had the highest pH (5.26), the highest potassium concentration (0.7%) and high nitrogen concentrations (1.32%). The composition of Compost 1 was 5 kg dry leaves + 500 g dry Kacip Fatimah + Slow Release Fertilizer (SRF). Results from this study showed that growing media is a key factor that leads to significant increment in phenolic compounds in L. pumila.
THE WELDABILITY OF LOW CARBON HIGH NIOBIUM STEELS DESIGNED FOR LINEPIPE AND GENERAL USE WITHIN THE HIPERC PROJECT

The HIPERC study was a European research project involving several steel companies and research organisations which examined the effects of alloying elements and processing conditions in low carbon (< 0.09 wt%), niobium containing (0.05 0.12 wt.%) steels. Laboratory-scale heats and pilot rolling trials simulating air and water-cooled plate production, as well as hot-rolled strip production, were made and the effects of C, Mn, Ni, Cu, Cr, Mo, Nb, Ti and B on transformation characteristics and temperatures of recrystallisation determined. Regression equations for the characterisation of microstructure, tensile and impact properties and for the weldability of these steels were derived. Several full commercial casts were made and processed into plate and coil-plate by three steel companies and pipe was produced from both products. The weldability of all these products was assessed and full scale fracture tests carried out. The project has shown that excellent combinations of strength, toughness and weldability can be obtained for a wide variety of applications.