Miracle-dz/UniFAR-FTUs huggingface dataset:
Aberrant Promoter Methylation of the ABCG2 Gene in Renal Carcinoma (cid:1)

ABCG2 is a ubiquitous ATP-binding cassette transmembrane protein that is important in clinical drug resistance. Little is known about the mechanism(s) regulating the expression of ABCG2 . We hypothesized that DNA methylation could play a role in the epigenetic regulation of ABCG2 gene expression. The promoter methylation status of three renal carcinoma cell lines was assessed with restriction enzyme digestion-coupled PCR and bisulﬁte genomic sequencing. Both UOK121 and UOK143, with known methylation of the VHL promoter, showed induction of ABCG2 expression after 5-aza-2 (cid:1) -deoxycytidine (5-aza-dC) treatment, suggesting that aberrant methylation of the ABCG2 gene was associated with gene silencing. In vitro methylation of the ABCG2 promoter-driven luciferase reporter vector resulted in a signiﬁcant inhibition of transcription. Our data suggested that the ABCG2 gene is regulated coordinately at both histone and DNA levels. A chromatin immunoprecipitation assay demonstrated that the methylated promoter in UOK121 and UOK143, but not the unmethylated one in UOK181, is associated with the m ethyl CpG b inding d omain proteins (MBDs), MBD2 and MeCP2. Histone deacetylase 1 and a corepressor, mSin3A, were identiﬁed binding to the promoter region containing the CpG island, thereby suppressing ABCG2 transcription. Reactivation of ABCG2 was achieved by treatment with 5-aza-dC, a demethylating agent, concomitant with the release of MBDs from the promoter. Furthermore, the association of methylated lysine 9 on histone H3, a hallmark of promoter methylation, with the promoter was reduced following 5-aza-dC treatment. These data suggest that DNA methylation-dependent formation of a repressor complex in the CpG island contributes to inactivation of ABCG2 anti-trimethyl-histone (Lys9), anti-HDAC1, and anti-MBD2 (Upstate); anti-MeCP2 (Abcam); anti-DNMT1, anti-DNMT3a, anti-DNMT3b, or anti-mSin3A (Santa Cruz Bio- technology). The DNA-protein complexes were sonicated using a Misonix son-icator MX2020 under conditions that gave a range in DNA fragments from 200 to 600 as determined by agarose gel
& (2006). Positive conceptions of perfectionism: Approaches, evidence, challenges. Personality and Social Psychology Review, 10, 295-319. Positive Conceptions of Perfectionism: Approaches, Evidence, Challenges

Almost 30 years ago, Hamachek (1978) suggested that two forms of perfectionism be distinguished, a positive form labeled „normal perfectionism” and a negative form labeled „neurotic perfectionism. ” Focusing on the positive, we present an overview of the different empirical conceptions of the two forms of perfectionism and present a common framework for the two basic approaches: the dimensional approach differentiating two dimensions of perfectionism (perfectionistic strivings and perfectionistic concerns) and the group-based approach differentiating two groups of perfectionists (healthy perfectionists and unhealthy perfectionists). Moreover, we review the evidence demonstrating that (a) perfectionistic strivings are associated with positive characteristics and (b) healthy perfectionists show higher levels of positive characteristics compared to unhealthy perfectionists and nonperfectionists. While questions on core facets, positive effects, and developmental antecedents of positive forms of perfectionism remain, our findings suggest that self-oriented perfectionistic strivings are positive, if perfectionists are not overly concerned about mistakes and negative evaluations by others.
New Insights into the Lpt Machinery for Lipopolysaccharide Transport to the Cell Surface: LptA-LptC Interaction and LptA Stability as Sensors of a Properly Assembled Transenvelope Complex (cid:1)

Lipopolysaccharide (LPS) is a major glycolipid present in the outer membrane (OM) of Gram-negative bacteria. The peculiar permeability barrier of the OM is due to the presence of LPS at the outer leaﬂet of this membrane that prevents many toxic compounds from entering the cell. In Escherichia coli LPS synthesized inside the cell is ﬁrst translocated over the inner membrane (IM) by the essential MsbA ﬂippase; then, seven essential Lpt proteins located in the IM (LptBCDF), in the periplasm (LptA), and in the OM (LptDE) are responsible for LPS transport across the periplasmic space and its assembly at the cell surface. The Lpt proteins constitute a transenvelope complex spanning IM and OM that appears to operate as a single device. We show here that in vivo LptA and LptC physically interact, forming a stable complex and, based on the analysis of loss-of-function mutations in LptC, we suggest that the C-terminal region of LptC is implicated in LptA binding. Moreover, we show that defects in Lpt components of
International Journal of Soft Computing and Engineering

his paper proposes a novel biometrics-based Single Sign On user authentication scheme in Telematics system, in order to enable to protect user account privacy. The focus during this work is to develop a secure and versatile multi-factor authentication to telematics environments with key management. Considering the massiveform ofdata users and documents inside the cloud, it's a necessityto allow multiple keywords inside the search request and are available documents inside the order of their connectedness to those keywords. Connected works on searchable encodingspecialize in single keyword search or man of science keyword search, and barely sort the search results. Among varied multikeyword linguistics, during this paper, for the first time we'vean inclinationto stipulate and solve the matter of multi-keyword stratified search over encrypted cloud knowledge, and establish a variety of privacy wants. Among varied multi-keyword linguistics, we tend to elect the economical similarity live of “coordinate matching”, i.e., as many matches as accomplishable, to effectively capture the connectedness of outsourced documents to the question keywords, and use “inner product similarity” to quantitatively decide such similarity live. For meeting the challenge of supporting multi-keyword linguistics while not privacy breaches, we'vean inclination to propose a basic arrange of MRSE mistreatment secure complex quantity computation. Then we'vean inclinationto supplya pair of improved MRSE schemes to achieve varied tight privacy requirements in a pair ofcompletelytotally different threat models. Thorough analysis work privacy and efficiency guarantees of projected schemes is given, and experiments on the real-world dataset show our projected schemes introduce low overhead on every computation and communication Keywords— MRSE, LNCS, PEKS
Foot-and-Mouth Disease Virus Assembly: Processing of Recombinant Capsid Precursor by Exogenous Protease Induces Self-Assembly of Pentamers In Vitro in a Myristoylation-Dependent Manner (cid:1)

The assembly of foot-and-mouth disease virus (FMDV) particles is poorly understood. In addition, there are important differences in the antigenic and receptor binding properties of virus assembly and dissociation intermediates, and these also remain unexplained. We have established an experimental model in which the antigenicity, receptor binding characteristics, and in vitro assembly of capsid precursor can be studied entirely from puriﬁed components. Recombinant capsid precursor protein (P1 region) was expressed in Escherichia coli as myristoylated or unmyristoylated protein. The protein sedimented in sucrose gradients at 5S and reacted with monoclonal antibodies which recognize conformational or linear antigen determinants on the virion surface. In addition, it bound the integrin (cid:1) v (cid:2) 6 , a cellular receptor for FMDV, indicating that unprocessed recombinant capsid precursor is both structurally and antigenically similar to native virus capsid. These characteristics were not dependent on the presence of 2A at the C terminus but were altered by N-terminal myristoylation and in mutant precursors which lacked VP4. Proteolytic processing of myristoylated precursor by recombinant FMDV 3C pro in vitro induced a shift in sedimentation from 5S to 12S, indicating assembly into pentameric capsid subunits. Nonmyristoylated precursor still assembled into higher-order structures after processing with 3C pro , but these particles sedimented in sucrose gradients at approximately 17S. In contrast, mutant precursors lacking VP4 were antigenically distinct, were unable to form pentamers, and had reduced capacity for binding integrin receptor. These studies demonstrate the utility of recombinant capsid precursor protein for investigating the initial stages of assembly of FMDV and other picornaviruses.
An Effective Approach for Web Services Recommendation Using Adaptive Weighting Algorithm with Temporal Features

: A Web service is a method of communication can taken place between two electronic devices over a network.Service Oriented applications are frequently constructed by adapting web services. Web services are recommended based on Quality of Service (QoS) factors that usually depends on network, location of user and web services. In the existing system, Collaborative Filtering (CF) is the one of the common methodemployed for making web services recommendation. However the users ratings are notreflect onservice recommendation. In this paper, we proposed an adaptive weighting algorithm with temporal features for web service recommendation. This method influence the information of user ratings and profile content of web services, a set of features will be designed and dynamically recommended by adaptive weighting method. Dynamic features are extracted using Time Series Analysis (TSA) algorithm. For similar neighbor selection and data modeling, k-means clustering algorithm is to be used.
The 1s2s2p2 5P1,2,3-1s2p3 5S20 Transition in Ne VII

The 1s2s2p2 5P1,2,3-1s2p3 5S20 Ne VII transitions have been observed in the vacuum-ultraviolet spectrum of a foil-excited neon beam. Their wavelengths and the fine structure follow well the trend recently developed for the isoelectronic series of negative Li through F VI. We compare these results to computations using MCDF-OL and MCDF-EAL methods. We also identify transitions from doubly-excited singlet states in Ne VII and note that some of these have been incorrectly classified in the literature.
Double-Blind Randomized Trial on the Ef ﬁ cacy of the Forced Prolonged Position for Treatment of Lateral Canal Benign Paroxysmal Positional Vertigo

Objectives/Hypothesis: The need for class I and II studies on the ef ﬁ cacy of liberatory maneuvers in the treatment of lateral canal benign paroxysmal positional vertigo (LC-BPPV) motivated the present double-blind randomized trial on the short-term ef ﬁ cacy of the forced prolonged position (FPP). Study Design: Double-blind, randomized controlled trial. Methods: Two hundred twenty-one patients with unilateral LC-BPPV met the inclusion criteria for a multicentric study. Patients were randomly assigned to treatment by FPP (116 subjects) or sham treatment (105 subjects). Subjects were followed up at 24 hours with the supine roll test by blinded examiners. Results: Among the sample, 67.4% and 32.6% of the patients showed respectively geotropic and apogeotropic variant of LC-BPPV. At the 24-hour follow-up, the effectiveness of FFP compared to the sham maneuver was, respectively, 57.8% versus 12.4% ( P < .0001) in the total sample, 76.9% versus 11.3% ( P < .0001) in the geotropic variant group, and 60.5% versus 17.6% ( P = .0003) in the apogeotropic variant group, including resolution or transformation to geotropic variant. Conclusions: FPP proved highly effective compared to the sham maneuver. The present class 2 study of the ef ﬁ cacy of the FPP changes the level of recommendation of the method for treating LC-BPPV into a strong one.
RMHP_A_291498 595..604

1Emergency Center, Hubei Clinical Research Center for Emergency and Resuscitation, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, People’s Republic of China; 2Emergency Department, Wuhan No. 1 Hospital, Wuhan, Hubei, 430022, People’s Republic of China; 3Information Center, Wuhan No. 1 Hospital, Wuhan, Hubei, 430022, People’s Republic of China; 4Gennlife (Beijing) Biotechnology Co. Ltd, Haidian, Beijing, 100080, People’s Republic of China
The Discovery Potential of Neutral Supersymmetric Higgs Bosons with Decay to Tau Pairs at the ATLAS Experiment

This work presents a study of the discovery potential for the neutral supersymmetric Higgs bosons h/A/H decaying to τ-pairs with the ATLAS experiment at the LHC. The study is based on Monte Carlo samples which are scaled to state-of-the-art cross sections. The analyses are designed assuming an integrated luminosity of 30 fb−1 and a center-of-mass energy of √ s = 14 TeV. The results are interpreted in the mmax h benchmark scenario. Two final states are analyzed: The dileptonic channel where the two τ-leptons decay to electrons or muons and the lepton-hadron channel where one τ decays to an electron or muon and the other τ decays to hadrons. The study of the dilepton channel is based completely on the detailed ATLAS simulation, the analysis of the lepton-hadron channel is based on the fast simulation. The collinear approximation is used to reconstruct the Higgs boson mass and its performance is studied. Cuts are optimized in order to discriminate the signal from background and to maximize the discovery potential given a certain Higgs boson mass hypothesis. In the lepton-hadron channel the selection is split into two analyses depending on the number of identified b-jets. Procedures to estimate the dominant backgrounds from data are studied. The shape and normalization of the Z → ττ background are estimated from Z → ll control regions. The tt̄ contributions to the signal regions are estimated from tt̄ control regions. The individual analyses are combined and sensitivity predictions are made depending on the Higgs boson mass mA and the coupling parameter tanβ . The light neutral MSSM Higgs bosons with mA = 150 GeV can be discovered when at least tanβ = 11 is realized in nature. The heavy neutral MSSM Higgs bosons with mA = 800 GeV can be discovered for tanβ ≥ 44. However, due to the large width of the reconstructed Higgs boson mass and the mass degeneration, only the sum of at least two of the three Higgs boson signals will be visible.
An Efficient Parallel Iterative Mapreduce Based Frequent Subgraph Mining Algorithm

: Mining frequent sub-graphs has attracted a great deal of attention in many areas, such as bioinformatics, the web data mining and social networks. There are many promising main memory-based techniques available in this area, but they lack scalability as the main memory is a bottleneck. Taking the massive data into consideration, traditional database systems like relational databases and object databases fail miserably with respect to on efficiency as frequent subgraph mining is computationally intensive. The proposed algorithm is parallel iterative Map-Reduce based frequent subgraph mining (PSGM), which in this work a frequent subgraph mining algorithm called PSGM which uses iterative Map-Reduce based framework. PSGM is complete as it returns all the frequent subgraphs for a given user-defined support and it is efficient as it applies all the optimizations that the latest FSM algorithms adopt. The experiments with real life and large synthetic datasets validate the effectiveness of PSGM for mining frequent sub-graphs from large graph datasets.
Inﬂuence of a Trans-Theoretical Model Based Intervention on Physical Activity in Hypertensive Patients: A Randomised Clinical Trial

Background: Although physical activity is necessary, but not performed by most hypertensive individuals. One of the most common theoretical frameworks used for understanding health behaviour change is the trans-theoretical model. Objectives: This study was conducted to evaluate the eﬀect of a trans-theoretical model based intervention on physical activity in hypertensive patients. Methods: Weconductedarandomizedclinicaltrialamong60hypertensivepatients(31experimentaland29control). Participants in experimental group received a fast walking intervention based on a trans-theoretical model. Physical activity stages of change, processes of change, frequency, duration and intensity of walking were measured at the onset, 3 and 6 months post-intervention. The data was analyzed using SPSS 22 software. Results: At 3 and 6 months post-intervention a signiﬁcant diﬀerence was observed between the experimental and control groups for physical activity stages of change, processes of change, duration and intensity of walking (P < 0.05). Also at 3 months post-interventionasigniﬁcantdiﬀerencewasobservedbetweentheexperimentalandcontrolgroupsforfrequencyof walking(P< 0.05). Conclusions: This study suggests that trans-theoretical model based intervention in hypertensive patients play important roles in an individual’s commitment to participate in physical activity.
AUS FINANZ- UND VERSICHERUNGSMATHEMATIK Energy Futures

We develop a model of Heath-Jarrow-Morton type for the forward price of energy that is driven by a d-dimensional L´evy process. The speciﬁcation of the forward yields a representation of the futures price of electricity as well. Moreover, a ﬁnite dimensional realization for the forward in Musiela parametrisation is assumed. The model is then ﬁtted using base-load prices of R (cid:13) PHELIX futures contracts traded on the European Energy Exchange. This calibration is performed using both ordinary and weighted least square methods and the results are compared. Moreover, possible applications of the model are given. Those comprise the simulation from the presented model and the estimation of market covariance structures.
MEASURING THE LEVEL OF QUALITY OF LIFE OF THE CUSTOMER IN THE IRAQI MINISTRY OF LABOR AND SOCIAL AFFAIRS : FIELD RESEARCH

This research aims to test the level of "quality of life of the customer" And its dimensions - Autonomy, Environmental mastery and economic empowerment, Self-acceptance and personal and physical growth, Positive and emotional relationships with others, Purpose in life- by comparing the opinions of officials and customers in the Ministry of Labor and Social Affairs of Iraq . the problem of research is determined by the following question: What is the role of officials in improving the quality of life of customers- beneficiaries- for the services of the ministry? So, the samples are represented by two parts, the first part consisted of a sample of (200) executives of social programs to measure the objective quality of customers, and the second part was a supporting sample of (100) beneficiaries of social programs to measure the subjective quality of themselves. The reality of the variables of the two samples was introduced and analysed. The significance differences were tested by using the two sample Mann Whitney Test. the results showed, the level of Quality of life according to opinions of the two samples, as well as the significant differences between the responses of the two samples. the most important recommendations focused on the recruitment of individuals with experience and give attention to improve the quality of life of the customer.
An Introduction to Generalized Linear Models

List of Figures and Tables Series Editor's Introduction Acknowledgments 1. Generalized Linear Models 2. Some Basic Modeling Concepts Categorical Independent Variables Essential Components of Regression Modeling 3. Classical Multiple Regression Model Assumptions and Modeling Approach Results of Regression Analysis Multiple Correlation Testing Hypotheses 4. Fundamentals of Generalized Linear Modeling Exponential Family of Distributions Classical Normal Regression Logistic Regression Poisson Regression Proportional Hazards Survival Model 5. Maximum Likelihood Estimation 6. Deviance and Goodness of Fit Using Deviances to Test Statistical Hypotheses Goodness of Fit Assessing Goodness of Fit by Residual Analysis 7. Logistic Regression Example of Logistic Regression 8. Poisson Regression Example of Poisson Regression Model 9. Survival Analysis Survival Time Distributions Exponential Survival Model Example of Exponential Survival Model Conclusions Appendix References Index About the Authors
OP-JAMI200218 1..10

Objective: Due to a complex set of processes involved with the recording of health information in the Electronic Health Records (EHRs), the truthfulness of EHR diagnosis records is questionable. We present a computational approach to estimate the probability that a single diagnosis record in the EHR reflects the true disease. Materials and Methods: Using EHR data on 18 diseases from the Mass General Brigham (MGB) Biobank, we develop generative classifiers on a small set of disease-agnostic features from EHRs that aim to represent Patients, pRoviders, and their Interactions within the healthcare SysteM (PRISM features). Results: We demonstrate that PRISM features and the generative PRISM classifiers are potent for estimating disease probabilities and exhibit generalizable and transferable distributional characteristics across diseases and patient populations. The joint probabilities we learn about diseases through the PRISM features via PRISM generative models are transferable and generalizable to multiple diseases. Discussion: The Generative Transfer Learning (GTL) approach with PRISM classifiers enables the scalable validation of computable phenotypes in EHRs without the need for domain-specific knowledge about specific disease processes. Conclusion: Probabilities computed from the generative PRISM classifier can enhance and accelerate applied Machine Learning research and discoveries with EHR data.
Mixing Beyond the SM from N f = 2 tmQCD and model independent constraints from the UTA

We present the first unquenched, continuum limit, lattice QCD results for the matrix elements of the operators describing neutral kaon oscillations in extensions of the Standard Model. Owing to the accuracy of our calculation on ∆S = 2 weak Hamiltonian matrix elements, we are able to provide a refined Unitarity Triangle analysis improving the bounds coming from model independent constraints on New Physics. In our non-perturbative computation we use a combination of Nf = 2 maximally twisted sea quarks and OsterwalderSeiler valence quarks in order to achieve both O(a)-improvement and continuum-like renormalization properties for the relevant four-fermion operators. The calculation of the renormalization constants has been performed non-perturbatively in the RI-MOM scheme. Based on simulations at four values of the lattice spacing and a number of quark masses we have extrapolated/interpolated our results to the continuum limit and physical light/strange quark masses.