Miracle-dz/UniFAR-FTUs huggingface dataset:
This information is current as Vaccinia Virus Ankara Boost Regimen Vaccinated with a DNA Prime/Modified Blood Lymphocytes from Rhesus Macaques Activity in Fresh, Unstimulated Peripheral Induction of AIDS Virus-Specific CTL

WatkinsAltman, Bernard Moss, Andrew J. McMichael and David I. Shipley, Jim Fuller, Tomas Hanke, Alessandro Sette, John D.Bianca R. Mothe, Susan Steffen, Jon E. Boyson, Tim Todd M. Allen, Thorsten U. Vogel, Deborah H. Fuller,http://www.jimmunol.org/content/164/9/4968J Immunol€2000; 164:4968-4978; ;Referenceshttp://www.jimmunol.org/content/164/9/4968.full#ref-list-1This article cites 72 articles, 37 of which you can access for free at: Subscriptionshttp://jimmunol.org/subscriptionsInformation about subscribing to The Journal of Immunology is online at: Permissionshttp://www.aai. org/ji/copyright.htmlSubmit copyright permission requests at: Email Alertshttp://jimmunol.org/cgi/alerts/etocReceive free email-alerts when new articles cite this article. Sign up at:
Phosphoinositide 3-Kinase Subunit of b Lacking the p85 Enhanced T Cell Proliferation in Mice

Phosphoinositide 3-kinase activation is important for lymphocyte proliferation and survival. Disrupting the gene that encodes the major phosphoinositide 3-kinase regulatory isoform p85 (cid:2) impairs B cell development and proliferation. However, T cell functions are intact in the absence of p85 (cid:2) . In this study, we test the hypothesis that the related isoform p85 (cid:1) is an essential regulatory subunit for T cell signaling. Unexpectedly, T cells lacking p85 (cid:1) showed a marked increase in proliferation and decreased death when stimulated with anti-CD3 plus IL-2. Both CD4 (cid:3) and CD8 (cid:3) T cells completed more cell divisions. Transcriptional proﬁling revealed reduced levels of caspase-6 mRNA in p85 (cid:1) -deﬁcient T cells, which was paralleled by reduced caspase-6 enzyme activity. Increased T cell accumulation was also observed in vivo following infection of p85 (cid:1) -deﬁcient mice with mouse hepatitis virus. Together, these results suggest a unique role for p85 (cid:1) in limiting T cell expansion. The Journal of Immunology, 2004, 172: 6615–6625. speciﬁc in p85 and a rabbit antiserum that recognizes all class I A PI3K regulatory isoforms (anti-pan-p85, 06-195; Upstate Bio- technology). To detect p85 (cid:2) , lysates were subjected to partial immu-nodepletion with mAb anti-p85 (cid:1) (three successive immunoprecipitations) before blotting with anti-pan-p85. As loading controls, mAbs speciﬁc for (cid:2) -actin or (cid:1) -tubulin (clones AC15 and DM1A; Sigma-Aldrich) were used. Band intensity was quantitated using NIH Image 1.61.
Allelism of IMPJ and GAL2 Genes of Saccharomyces cerevisiae

Cloning and characterization of the previously described Saccharomyces cerevisiae IMP) gene, which was assumed to be a nuclear determinant involved in the nucleomitochondrial control of the utilization of galactose, demonstrate allelism to the GAL2 gene. Galactose metabolism does not necessarily involve the induction of the specific transport system coded by GAL2/IMP1, because a null mutant takes up galactose and grows on it. Data on galactose uptake are presented, and the dependence on ATP for constitutive and inducible galactose transport is discussed. These results can account for the inability of impl/gal2 mutants to grow on galactose in a respiration-deficient background. Under these conditions, uptake was affected at the functional level but not at the biosynthetic level.
Impact of Local Stress Distribution in a Silicon Chip Mounted by Area-Arrayed Copper Pillar Wafer-Level Packaging Technology on Analog-Circuit Performance

The local stress distribution in a silicon chip encapsulated in an area-arrayed copper pillar-type flip-chip package was evaluated using specially designed test chips. Stress is sensed using piezoelectric resistors, which are p-type and n-type diffusion resistors embedded in a silicon chip measuring 7.8 mm $\times7.8$ mm. Each piezoelectric resistor measures approximately 0.03 mm in length and 0.002 mm in width, which is sufficiently small to measure the local stress distribution near each copper pillar. As a result of this study, it is revealed that the copper pillars have an impact on the local stress distribution in silicon chips, in particular producing a large stress gradient near the copper pillar edge, compared to the conventional wire-bonded packages. Since large stress gradients disturb pair characteristics of analog circuits, in order to design a high-precision integrated circuit employing a copper pillar-type flip-chip package, a specific regulation method to avoid overlap with the copper pillars is needed to maintain the accuracy of the analog circuits.
Screening for Depression in Adolescents with Sickle Cell Disease

Background: Sickle Cell Disease (SCD) is a chronic hematologic disease. Adults with SCD are known to suffer higher rates of depression. Little is known about rates in adolescents with SCD. Our study examined prevalence of depression in adolescent SCD patients at a sickle cell clinic following integration of a depression screening protocol. Methods: A protocol was developed to identify, screen, and refer patients. Patients ages 12 years and older were eligible for screening using the 9-question Patient Health Questionnaire (PHQ-9). Data collected included depression screen scores, age, sex, hemoglobin, hydroxyurea use, history of stroke, and previous mental health diagnosis. The study spanned May 2018 to May 2019. Results: 52 patients were screened. Less than 10% had a prior diagnosis of depression and had an average score of 7 (+/- 3.54), compared to 4.21 (+/-4.16; p=0.128) for those without. <10% had a diagnosis of chronic pain. 40% screened positive for depression: 65% mild, 29% moderate, and 6% severe. 1 patient expressed active suicidality. More females screened positive for depression (54% vs . 23%; p=0.023) and had higher PHQ-9 scores (5.96 vs . 3; p=0.009). There was no significant difference in PHQ-9 scores based on sickle cell genotype, hemoglobin, hydroxyurea use, or history of stroke or chronic pain. Conclusions: Adolescent patients with SCD may be underdiagnosed for depression. Screening in hematology clinics can be valuable in identifying and referring patients. Studies involving larger cohorts are needed to determine higher risk subgroups of patients.
Entanglement entropy of Wilson loops: Holography and matrix models

A half-BPS circular Wilson loop in N = 4 SU ( N ) supersymmetric Yang-Mills theory in an arbitrary representation is described by a Gaussian matrix model with a particular insertion. The additional entanglement entropy of a spherical region in the presence of such a loop was recently computed by Lewkowycz and Maldacena using exact matrix model results. In this note we utilize the supergravity solutions that are dual to such Wilson loops in a representation with order N 2 boxes to calculate this entropy holographically. Employing the matrix model results of Gomis, Matsuura, Okuda and Trancanelli we express this holographic entanglement entropy in a form that can be compared with the calculation of Lewkowycz and Maldacena. We ﬁnd complete agreement between the matrix model and holographic calculations.
A two-tier proportional hazards regression model with change-points in the baseline function

In this article, we consider a new regression model for counting processes under a proportional hazards assumption. This model is motivated by the need of understanding the evolution of the booking process of a railway company. The main novelty of the approach consists in assuming that the baseline hazard function is piecewise constant. Hence, the parameters of the model can be separated into two diﬀerent types: parameters that measure the inﬂuence of the covariates, and parameters from a (multiple) change-point model for the baseline. Cox’s semiparametric regression can be seen as a limit case of our model. We develop an iterative procedure to estimate the diﬀerent parameters, and a test procedure that allows to perform change-point detection in the baseline. Our technique is supported by simulation studies and a real data analysis, which show that our model can be a reasonable alternative to Cox’s regression model, particularly in the presence of tied event times.
in Computer

– This research paper objective is to provide analysis of classifying the data set using novel naïve classification algorithm. Here this algorithm applied on two dataset which are the first one is training data set and other one is test data set, and this experiment attempts to compare the classification and accuracy of the proposed algorithm with the two dataset . Several constraints used for analytical purpose which are classification accuracy, True Positive Rate, False positive Rate sensitivity, Precision, Recall and f-measure using confusion matrix. Finally results are given in the tabular form to facilitate comparative analysis. From this study it is found that this algorithm given good result for both train as well as test data set but more accurate for train set. Section I discussed the general approach of classification, section II discussed Literature review, section III discussed the proposed work about the performance of train and test dataset, result and discussion briefed in the section IV and conclusion in section V.
METHOD DEVELOPMENT AND VALIDATION OF BUMETANIDE BY UV SPECTROPHOTOMETRIC METHOD IN BULK AND PHARMACEUTICAL DOSAGE

Analytical method development and validation play important role in the discovery, development and manufacture of pharmaceuticals. A simple, rapid and reproducible UV- spectrophotometric method for the quantitative determination of bumetanide in tablet formulation was developed and validated in the present work. Parameters such as linearity, precision and accuracy were studied according to ICH guidelines. The method A was carried out with borate buffer of pH 9 and method B was carried out with phosphate buffer of pH 7. The wavelength 252nm was selected for the estimation of drug using distilled water as a solvent. The drug obeyed Beer-Lambert’s law over the concentration range 5-75µg/ml. The accuracy of the method was assessed by recovery studies and was found between 99.03 – 100.3% for method A and 99.5 – 100.5% for method B. The method was successfully applied for routine analysis of bumetanide drug in formulations.
Allospecific Tolerance Inhibition of Mixed Chimerism and Characterization of Virus-Mediated

Simultaneous blockade of the CD28 and CD40 T cell costimulatory pathways has been shown to effectively promote skin allograft survival in mice. Furthermore, blockade of one or both of these pathways has played a central role in the development of strategies to induce mixed hematopoietic chimerism and allospeciﬁc tolerance. It has recently been observed that the beneﬁcial effects of CD40 blockade and donor splenocytes in prolonging skin graft survival can be abrogated by some viral infections, including lymphocytic choriomeningitis virus (LCMV). In this study, we show that LCMV infection prevents prolonged allograft survival following CD28/CD40 combined blockade. We further show that LCMV prevents the induction of allospeciﬁc tolerance and mixed hematopoietic chimerism, while delay of infection for 3–4 wk posttransplant has no effect on tolerance induction. Because of reports of anti-H-2 d activity following LCMV infection, we assayed the ability of LCMV-speciﬁc T cells to respond to alloantigen at a single cell level. Although we conﬁrm that LCMV infection induces the generation of alloreactive cells, we also demonstrate that LCMV-speciﬁc T cells do not divide in response to alloantigen. The alloresponse suppressed by costimulation blockade is restored by LCMV infection and correlates with increased dendritic cell maturation. We hypothesize that the costimulation blockade-resistant rejection mediated by LCMV could be partly attributable to the up-regulation of alternative costimulatory pathways subsequent to LCMV-induced dendritic cell maturation. The Journal of Immunology, 2001, 167: 4987–4995. present a detailed quantitative assessment of allogeneic and antiviral responses using a graft-vs-host disease (GVHD) model and MHC tetramer analysis, as well as ELISPOT and intracellular IFN- (cid:1) staining. Although LCMV infection allows for the generation of costimulation blockade-resistant alloresponses, cross-reac-tivity of LCMV-speciﬁc CD8 T cells to H-2 d alloantigen is minimal at a single cell level. Although mice receiving LCMV infection without a skin graft do generate high numbers of alloreactive cells at the peak of the response, their presence is largely associated with acute infection, as they are reduced 50- to 100-fold by day 30 postinfection, while normal LCMV-speciﬁc T cells are reduced 10- to 12-fold over the same time period. To explore alternative reasons for the virus-induced abrogation of tolerance induction, we assess dendritic cell activation in the spleen following transplantation and concurrent viral infection. We show that LCMV drives dendritic cell maturation, regardless of the presence of costimulation blockade. We propose that one possible explanation for the LCMV-induced rejection of skin allografts is that infection leads to dendritic cell maturation and an increased capacity for stimulating CD28/CD40-independent T cell responses.
Nontoxic radioactive Listeria at is a highly effective therapy against metastatic pancreatic cancer

, No signi ﬁ cant improvement in therapy of pancreatic cancer has been reported over the last 25 y, underscoring the urgent need for new alternative therapies. Here, we coupled a radioisotope, 188 Rhenium, to an attenuated (at) live Listeria monocytogenes ( Listeria at ) using Listeria -binding antibodies, thus creating a unique radioactive Listeria at (RL). We then demonstrated in a highly metastatic pancreatic mouse tumor model (Panc-02) that RL delivered radioactivity to the metastases and less abundantly to primary tumors in vivo, with- out harming normal cells. This result was possible because Listeria at was ef ﬁ ciently cleared by the immune system in normal tissues but not in the heavily immune-suppressed microenvironment of metas- tases and primary tumor. Multiple treatments with low doses of the RL resulted in a dramatic decrease in the number of metastases ( ∼ 90%) compared with control groups in the Panc-02 model. This is the ﬁ rst report of using live attenuated bacteria delivering a highly radioactive payload to the metastases, resulting in killing tumor cells in vivo without harming normal cells. The nontoxic RL treatment is attractive for clinical development as a therapy to prevent pan- creatic cancer recurrence and metastases.
Large Scale Vertebrae Segmentation Challenge: Structured description of the challenge design

Provide a summary of the challenge purpose. This should include a general introduction in the topic from both a biomedical as well as from a technical point of view and clearly state the envisioned technical and/or biomedical impact of the challenge. A primary step in automated quantification of spinal morphology and pathology is vertebral labelling and segmentation. Aimed at these tasks, the first iteration of the 'Large Scale Vertebrae Segmentation Challenge' (VerSe'19) was held at MICCAI 2019 and received considerable participation from the community (>250 registrations and data downloads, 20 participating teams). With its first iteration, VerSe addressed a severe shortage of publicly-available, large, accurately annotated CT spine data in the community by releasing 160 CT scans and their voxel-level annotations comprised of a large variety in fields of view, spatial resolutions, spinal and vertebral pathologies, collected over several scanners from two major vendors. Building on the data, experience, and learning from VerSe'19, we propose to organise a second iteration for of the Large Scale Vertebrae Segmentation Challenge (VerSe'20) at MICCAI 2020. With VerSe'20, we aim to work with 300 CT scans (~100% increase over its previous iteration). While retaining the richness of its predecessor, the data will now be multi-centre with five different institutions and and all four major scanner manufacturers. Additionally, challenging the learning algorithms, focus is given to include atypical anatomies such as transitional vertebrae and additional vertebrae such as L6. With clinical-translation being the primary objective of data preparation, we believe the algorithms using this data would be more robust and generalisable.
Theoretical perspective of unusual purchasing tendencies during pandemic situation of covid-19

During the emergence of COVID-19 an unusual consumer behavior were noticed Individuals and groups were seen with unusual behavior Present study conceptualized the behavior of consumers during pandemic situation of COVID-19 Further the study has suggested the framework and considered five (5) constructs with five hypotheses Pandemic situation is considered as the antecedent, online information source exposure considered as mediator between perceived severity & Cyberchondria and pandemic situation of COVID-19 Intention to make unusual purchases suggested as consequence of the considered framework Study is purely based on concepts and suggestions Study further provided with extensive and holistically relevant literature © 2020 Ubiquity Press All rights reserved
Rapid identification of mutations in GJC2 in four limb primary lymphoedema using whole exome sequencing combined with linkage analysis

Primary lymphoedema describes a chronic, frequently progressive, failure of lymphatic drainage. We ascertained a multigenerational family in which eight individuals developed post natal lymphoedema of all four limbs. Linkage analysis revealed cosegregation of a 16.1Mb haplotype on chromosome 1q42 that contained 173 known or predicted genes. We undertook whole exome sequencing in a single affected individual and focused the search for the causative variant to within the linkage interval. This approach revealed two novel nonsynonymous single nucleotide substitutions within the chromosome 1 locus, in NVL and GJC2. We sequenced NVL and GJC2 in an additional cohort of individuals with a similar phenotype and found nonsynonymous variants in GJC2 in four additional families. This report demonstrates the power of exome sequencing efficiently applied to a traditional positional cloning pipeline in disease gene discovery.
Sequencing and Analysis of Common Bean ESTs. Building a Foundation for Functional Genomics 1[w]

Although common bean ( Phaseolus vulgaris ) is the most important grain legume in the developing world for human consumption, few genomic resources exist for this species. The objectives of this research were to develop expressed sequence tag (EST) resources for common bean and assess nodule gene expression through high-density macroarrays. We sequenced a total of 21,026 ESTs derived from 5 different cDNA libraries, including nitrogen-ﬁxing root nodules, phosphorus-deﬁcient roots, developing pods, and leaves of the Mesoamerican genotype, Negro Jamapa 81. The ﬁfth source of ESTs was a leaf cDNA library derived from the Andean genotype, G19833. Of the total high-quality sequences, 5,703 ESTs were classiﬁed as singletons, while 10,078 were assembled into 2,226 contigs producing a nonredundant set of 7,969 different transcripts. Sequences were grouped according to 4 main categories, metabolism (34%), cell cycle and plant development (11%), interaction with the environment (19%), and unknown function (36%), and further subdivided into 15 subcategories. Comparisons to other legume EST projects suggest that an entirely different repertoire of genes is expressed in common bean nodules. Phaseolus-speciﬁc contigs, gene families, and single nucleotide polymorphisms were also identiﬁed from the EST collection. Functional aspects of individual bean organs were reﬂected by the 20 contigs from each library composed of the most redundant ESTs. The abundance of transcripts corresponding to selected contigs was evaluated by RNA blots to determine whether gene expression determined by laboratory methods correlated with in silico expression. Evaluation of root nodule gene expression by macroarrays and RNA blots showed that genes related to nitrogen and carbon metabolism are integrated for ureide production. Resources developed in this project provide genetic and genomic tools for an international consortium devoted to bean improvement. the normalization of quantiﬁed the normalized intensities Excel WA) ﬁles linked to the corre- sponding cDNA clone. In order to work with highly reproducible experiments, linear regression analysis was performed for each pair of membrane replicas; only those replicas for which the linear model could explain at least 80% of the variation (determination coefﬁcient r 2 $ 0.8) taken into consideration. This yielded a total of 3, 3, 2, 2, and 2 well-correlated replicas nodule, root, leaf, stem, and pod, respectively. reliably intensity/ background ratios than 1.5 through all related parallel hybridizations.
A RARE CASE OF LIVER CIRRHOSIS AND PORTAL HYPERTENSION WITH HUGE SPLENOMEGALY, HYPERSPLENISM & SPLENIC PSEUDOCYST

Portal hypertension is a common problem in our country. It occurs as a complication to periportal fibrosis that result from schistomiasis, it a medical condition unless complicated. the complications may manifest as hyperactivity of the spleen and destruction of all blood cell types that result in pancytopenia as in this case. The enlarged spleen may undergo some degenerative changes such as splenic infarction that complicated by pseudocyst as in this case. A case reported of 56 yrs old known case of periportal fibrosis, portal hypertension and liver cirrhosis complicated by huge splenomegaly, hypersplenism and post infarction splenic cyst which develop rapidly over period of 10 days during hospital admission. It’s very rare to find all these findings on the same pt and to our knowledge there’s no literature reported similar case.
Reservation-Based 3D Intersection Traffic Control System for Autonomous Unmanned Aerial Vehicles

We present a three-dimensional (3D) intersection traffic management platform for small autonomous Unmanned Aerial Vehicles (UAVs), particularly quadcopters, in urban airspace. Assuming many autonomous UAVs are approaching a shared airspace, where UAVs have varying sources and destinations, we propose a system model for a 3D intersection that aims to provide safe and systematic management of UAVs. We also devised a scheduling scheme to ensure that the intersection is efficiently utilized and that there are no collisions among the UAVs in the intersection. The scheduling scheme applies the reservation-based approach, which is sensitive to the sequence of the UAVs in scheduling, thus genetic algorithm is used to determine the best sequence of the UAVs. Simulations were performed to evaluate the efficiency of the system. We also show through the simulations that our scheduling scheme reduces the UAVs’ average time in the system by 27 percent compared with when the UAVs are scheduled in a first-come, first-served manner for the highly crowded intersection.
Structured-signal recovery from single-bit measurements

—1-bit compressed sensing was introduced by Boufounos and Baraniuk in 2008 as a model of extreme quantization; only the sign of each measurement is retained. Recent theoretical and algorithmic advances, combined with the ease of hardware implementation, show that it is an effective method of signal acquisition. Surprisingly, in the high-noise regime there is almost no information loss from 1-bit quantization. We review and revise recent results, and compare to closely related statistical problems: sparse binary regression and binary matrix completion.