Miracle-dz/UniFAR-FTUs huggingface dataset:
Fully Integrated PET/MR Imaging for the Assessment of the Relationship Between Functional Connectivity and Glucose Metabolic Rate

In the past, determination of absolute values of cerebral metabolic rate of glucose (CMRGlc) in clinical routine was rarely carried out due to the invasive nature of arterial sampling. With the advent of combined PET/MR imaging technology, CMRGlc values can be obtained non-invasively, thereby providing the opportunity to take advantage of fully quantitative data in clinical routine. However, CMRGlc values display high physiological variability, presumably due to fluctuations in the intrinsic activity of the brain at rest. To reduce CMRGlc variability associated with these fluctuations, the objective of this study was to determine whether functional connectivity measures derived from resting-state fMRI (rs-fMRI) could be used to correct for these fluctuations in intrinsic brain activity. Methods: We studied 10 healthy volunteers who underwent a test-retest dynamic [18F]FDG-PET study using a fully integrated PET/MR system (Siemens Biograph mMR). To validate the non-invasive derivation of an image-derived input function based on combined analysis of PET and MR data, arterial blood samples were obtained. Using the arterial input function (AIF), parametric images representing CMRGlc were determined using the Patlak graphical approach. Both directed functional connectivity (dFC) and undirected functional connectivity (uFC) were determined between nodes in six major networks (Default mode network, Salience, L/R Executive, Attention, and Sensory-motor network) using either a bivariate-correlation (R coefficient) or a Multi-Variate AutoRegressive (MVAR) model. In addition, the performance of a regional connectivity measure, the fractional amplitude of low frequency fluctuations (fALFF), was also investigated. Results: The average intrasubject variability for CMRGlc values between test and retest was determined as (14 ±8%) with an average inter-subject variability of 25% at test and 15% at retest. The average CMRGlc value (umol/100 g/min) across all networks was 39 ±10 at test and increased slightly to 43 ±6 at retest. The R, MVAR and fALFF coefficients showed relatively large test-retest variability in comparison to the inter-subjects variability, resulting in poor reliability (intraclass correlation in the range of 0.11–0.65). More importantly, no significant relationship was found between the R coefficients (for uFC), MVAR coefficients (for dFC) or fALFF and corresponding CMRGlc values for any of the six major networks. Discussion: Measurement of functional connectivity within established brain networks did not provide a means to decrease the inter- or intrasubject variability of CMRGlc values. As such, our results indicate that connectivity measured derived from rs-fMRI acquired contemporaneously with PET imaging are not suited for correction of CMRGlc variability associated with intrinsic fluctuations of resting-state brain activity. Thus, given the observed substantial inter- and intrasubject variability of CMRGlc values, the relevance of absolute quantification for clinical routine is presently uncertain.
A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

Ascomycota and Basidiomycota are two closely related phyla and fungi in two phyla share some common morphological developmental process during fruiting body formation. In Neurospora crassa, the Gβ-like protein CPC-2 with a seven-WD40 repeat domain was previously reported. By transforming CPC-2 ortholog encoding genes, from 7 different fungal species across Ascomycota and Basidiomycota, into the cpc-2 deletion mutant of N. crassa, we demonstrate that all tested CPC-2 ortholog genes were able to complement the defects of the cpc-2 deletion mutant in sexual development, indicating that CPC-2 proteins from Ascomycota and Basidiomycota have the similar cellular function. Using Flammulina velutipes as a model system for mushroom species, the CPC-2 ortholog FvCPC2 was characterized. Fvcpc2 increased transcription during fruiting body development. Knockdown of Fvcpc2 by RNAi completely impaired fruiting body formation. In three Fvcpc2 knockdown mutants, transcriptional levels of genes encoding adenylate cyclase and protein kinase A catalytic subunit were significantly lower and colony growth became slower than wild type. The addition of cAMP or the PKA-activator 8-Bromo-cAMP into the medium restored the Fvcpc2 knockdown mutants to the wild-type colony growth phenotype, suggesting that the involvement of cAMP production in the regulatory mechanisms of FvCPC2. Knockdown of Fvcpc2 also weakened transcriptional responses to sexual development induction by some genes related to fruiting body development, including 4 jacalin-related lectin encoding genes, 4 hydrophobin encoding genes, and 3 functionally-unknown genes, suggesting the participation of these genes in the mechanisms by which FvCPC2 regulates fruiting body development. All three Fvcpc2 overexpression strains displayed increased mushroom yield and shortened cultivation time compared to wild type, suggesting that Fvcpc2 can be a promising reference gene for Winter Mushroom breeding. Since the orthologs of FvCPC2 were highly conserved and specifically expressed during fruiting body development in different edible mushrooms, genes encoding FvCPC2 orthologs in other mushroom species also have potential application in breeding.
Diversity Profiling of Grapevine Microbial Endosphere and Antagonistic Potential of Endophytic Pseudomonas Against Grapevine Trunk Diseases

Grapevine trunk diseases (GTDs) are a serious problem of grapevines worldwide. The microbiota of the grapevine endosphere comprises prokaryotic and eukaryotic endophytes, which may form varied relationships with the host plant from symbiotic to pathogenic. To explore the interaction between grapevine endophytic bacteria and GTDs, the endomicrobiome associated with grapevine wood was characterized using next-generation Illumina sequencing. Wood samples were collected from grapevine trunks with and without external symptoms of GTD (cankers) from two vineyards in the Hunter Valley and Hilltops, NSW, Australia and metagenomic characterization of the endophytic community was conducted using the 16S rRNA gene (341F/806R) and ITS (1F/2R) sequences. Among the important GTD pathogens, Phaeomoniella, Phaeoacremonium, Diplodia and Cryptovalsa species were found to be abundant in both symptomatic and asymptomatic grapevines from both vineyards. Eutypa lata and Neofusicoccum parvum, two important GTD pathogens, were detected in low numbers in Hilltops and the Hunter Valley, respectively. Interestingly, Pseudomonas dominated the bacterial community in canker-free grapevine tissues in both locations, comprising 56–74% of the total bacterial population. In contrast, the Pseudomonas population in grapevines with cankers was significantly lower, representing 29 and 2% of the bacterial community in Hilltops and the Hunter Valley, respectively. The presence of Pseudomonas in healthy grapevine tissues indicates its ability to colonize and survive in the grapevine. The potential of Pseudomonas spp. as biocontrol agents against GTD pathogens was also explored. Dual culture tests with isolated fluorescent Pseudomonas against mycelial discs of nine Botryosphaeria dieback, three Eutypa dieback, and two Esca/Petri disease pathogens, revealed antagonistic activity for 10 Pseudomonas strains. These results suggest the potential of Pseudomonas species from grapevine wood to be used as biocontrol agents to manage certain GTD pathogens.
TRPV1 Tunes Optic Nerve Axon Excitability in Glaucoma

The transient receptor potential vanilloid member 1 (TRPV1) in the central nervous system may contribute to homeostatic plasticity by regulating intracellular Ca2+, which becomes unbalanced in age-related neurodegenerative diseases, including Alzheimer’s and Huntington’s. Glaucomatous optic neuropathy – the world’s leading cause of irreversible blindness – involves progressive degeneration of retinal ganglion cell (RGC) axons in the optic nerve through sensitivity to stress related to intraocular pressure (IOP). In models of glaucoma, genetic deletion of TRPV1 (Trpv1–/–) accelerates RGC axonopathy in the optic projection, whereas TRPV1 activation modulates RGC membrane polarization. In continuation of these studies, here, we found that Trpv1–/– increases the compound action potential (CAP) of optic nerves subjected to short-term elevations in IOP. This IOP-induced increase in CAP was not directly due to TRPV1 channels in the optic nerve, because the TRPV1-selective antagonist iodoresiniferatoxin had no effect on the CAP for wild-type optic nerve. Rather, the enhanced CAP in Trpv1–/– optic nerve was associated with increased expression of the voltage-gated sodium channel subunit 1.6 (NaV1.6) in longer nodes of Ranvier within RGC axons, rendering Trpv1–/– optic nerve relatively insensitive to NaV1.6 antagonism via 4,9-anhydrotetrodotoxin. These results indicate that with short-term elevations in IOP, Trpv1–/– increases axon excitability through greater NaV1.6 localization within longer nodes. In neurodegenerative disease, native TRPV1 may tune NaV expression in neurons under stress to match excitability to available metabolic resources.
Dissociating Profiles of Social Cognitive Disturbances Between Mixed Personality and Anxiety Disorder

Background An emerging body of research has begun to elucidate disturbances to social cognition in specific personality disorders (PDs). No research has been conducted on patients with Mixed Personality Disorder (MPD), however, who meet multiple diagnostic criteria. Further, very few studies have compared social cognition between patients with PD and those presenting with symptomatic diagnoses that co-occur with personality pathologies, such as anxiety disorder (AD). The aim of this study was to provide a detailed characterization of deficits to various aspects of social cognition in MPD and dissociate impairments specific to MPD from those exhibited by patients with AD who differ in the severity of personality pathology. Method Building on our previous research, we administered a large battery of self-report and performance-based measures of social cognition to age-, sex- and education-matched groups of patients with MPD or AD, and healthy control participants (HCs; n = 29, 23, and 54, respectively). This permitted a detailed profiling of these clinical groups according to impairments in emotion recognition and regulation, imitative control, low-level visual perspective taking, and empathic awareness and expression. Results The MPD group demonstrated poorer emotion recognition for negative facial expressions relative to both HCs and AD. Compared with HCs, both clinical groups also performed significantly worse in visual perspective taking and interference resolution, and reported higher personal distress when empathizing and more state-oriented emotion regulation. Conclusion We interpret our results to reflect dysfunctional cognitive control that is common to patients with both MPD and AD. Given the patterns of affective dispositions that characterize these two diagnostic groups, we suggest that prolonged negative affectivity is associated with inflexible styles of emotion regulation and attribution. This might potentiate the interpersonal dysfunction exhibited in MPD, particularly in negatively valenced and challenging social situations.
Effect of intracanal calcium hydroxide dressing on the push out bond strength of AH Plus, MTA Fillapex®, and endosequence BC sealer

Aim: This study aimed to compare the effect of intracanal calcium hydroxide dressing on the push-out bond strength of AH Plus, mineral trioxide aggregate (MTA) Fillapex®, and endosequence BC sealer. Methodology: Sixty single-rooted human mandibular premolars were decoronated and subjected to biomechanical preparation with the help of a ProTaper rotary file up to size F2. Half of the samples worked as control and the other half that worked as experimental group was treated with calcium hydroxide intracanal medicament. All the samples were divided into three groups (n = 20), according to the sealer used. The samples were obturated using endosequence BC sealer, AH Plus, and MTA Fillapex. Each sample was sectioned horizontally into three parts: cervical, middle, and apical. The sample so prepared was subjected to universal testing machine for the evaluation of the push-out bond strength of the sealers. Results: Endosequence BC sealer showed the best results, followed by AH Plus sealer and MTA Fillapex sealer. In addition, the push-out bond strength decreased significantly after the use of intracanal calcium hydroxide medicament (ApexCal). Push-out bond strength also decreased from cervical to middle followed by apical third. Conclusion: Calcium hydroxide intracanal medicament dressing reduced the push out bond strength of all the three sealers used in the study.
Opinions of Education Administrators and Teachers on Vegetation Geography: Comparative Case Study*

Abstract Vegetation geography subjects are believed to be barely taught in secondary schools.Yet it is a major defect in terms of environmental education. The assessment of opinions of senior administrators, high school principals, and geography and biology teachers has a high significance to eliminate such defect. In this study the qualitative research method was used. Semi- structured interview was done to gather the data and the content analysis was employed. While there is a small number of studies on vegetation geography in Cyprus, there is none on such education. The existing studies have botanical basis and thus do not have the nature to be interpreted from environmental elements and geographical aspects. The limited vegetation subjects given in the geography and biology text books are far from reflecting the interaction of natural vegetation of Cyprus with climate, land forms and human factors. Opinions stated in the study shows the importance of vegetation geography in North Cyprus. This study is the first in the literature
Compact Adaptively Secure ABE from k-Lin: Beyond NC1 and towards NL

We present a new general framework for constructing compact and adaptively secure attribute-based encryption (ABE) schemes from k -Lin in asymmetric bilinear pairing groups. Previously, the only construction [Kowalczyk and Wee, Eurocrypt ’19] that simultaneously achieves compactness and adaptive security from static assumptions supports policies represented by Boolean formulae . Our framework enables supporting more expressive policies represented by arithmetic branching programs . Our framework extends to ABE for policies represented by uniform models of computation such as Turing machines. Such policies enjoy the feature of being applicable to attributes of arbitrary lengths. We obtain the ﬁrst compact adaptively secure ABE for deterministic and non-deterministic ﬁnite automata (DFA and NFA) from k -Lin, previously unknown from any static assumptions. Beyond ﬁnite automata, we obtain the ﬁrst ABE for large classes of uniform computation, captured by deterministic and non-deterministic logspace Turing machines (the complexity classes L and NL ) based on k -Lin. Our ABE scheme has compact secret keys of size linear in the description size of the Turing machine M . The ciphertext size grows linearly in the input length, but also linearly in the time complexity, and exponentially in the space complexity. Irrespective of compactness, we stress that our scheme is the ﬁrst that supports large classes of Turing machines based solely on standard assumptions. In comparison, previous ABE for general Turing machines all rely on strong primitives related to indistinguishability obfuscation.
Introduction

Researchers and developers of educational software have experimented with natural language processing (NLP) capabilities and related technologies since the 1960's. Automated essay scoring was perhaps the first application of this kind (Page 1966). Over a decade later, Writer's Workbench, a text-editing application, was developed as a tool for classroom teachers (MacDonald, Frase, Gingrich and Keenan 1982). Intelligent tutoring applications, though more in the spirit of artificial intelligence, were also being developed during this time (Carbonell 1970; Brown, Burton and Bell 1974; Stevens and Collins 1977; Burton and Brown 1982; Clancy 1987).
Is the Mini-Mental State Examination (MMSE) useful for eligibility screening of research participants with substance use disorder?

Introduction: the MMSE is used for eligibility screening of potential research participants diagnosed with substance use disorder (SUD), as abilities needed to provide a valid informed consent or accurate information could be impaired in these clinical populations. Knowledge about the capacity of the MMSE to detect impairment in these abilities or at least to assess impact of SUD on its total score, however, is rather diffuse. This has important ethical and methodological implications. Objective: to analyze effects of SUD only, main substances of abuse, age of onset of substance use, recent substance use, and psychiatric comorbidity upon MMSE outcome. The overall purpose of the study was to assess the utility of the MMSE for eligibility screening of potential research participants with SUD. Method: individuals were recruited from residential facilities for substance use treatment. A demographic questionnaire, MMSE and Mini International Neuropsychiatric Interview were used. Results: A total of 601 participants were gathered for main analysis. Controlling for education, no differences in MMSE score were detected within main substances of abuse (F=1.25[4,529], p=.28), nor between SUD only versus SUD with psychiatric comorbidity (F=.58[1,597], p=.44). Effects of age of onset and recent use of specific substances upon MMSE score were also absent. Discussion and conclusions: If there is some cognitive impairment in this clinical population, it may not be pertinently assessed by the MMSE, thus casting doubt on its pertinence for eligibility screening.
QTL mapping for quality traits using a high-density genetic map of wheat

Protein- and starch-related quality traits, which are quantitatively inherited and significantly influenced by the environment, are critical determinants of the end-use quality of wheat. We constructed a high-density genetic map containing 10,739 loci (5,399 unique loci) using a set of 184 recombinant inbred lines (RILs) derived from a cross of ‘Tainong 18 × Linmai 6’ (TL-RILs). In this study, a quantitative trait loci (QTLs) analysis was used to examine the genetic control of grain protein content, sedimentation value, farinograph parameters, falling number and the performance of the starch pasting properties using TL-RILs grown in a field for three years. A total of 106 QTLs for 13 quality traits were detected, distributed on the 21 chromosomes. Of these, 38 and 68 QTLs for protein- and starch-related traits, respectively, were detected in three environments and their average values (AV). Twenty-six relatively high-frequency QTLs (RHF-QTLs) that were detected in more than two environments. Twelve stable QTL clusters containing at least one RHF-QTL were detected and classified into three types: detected only for protein-related traits (type I), detected only for starch-related traits (type II), and detected for both protein- and starch-related traits (type III). A total of 339 markers flanked with 11 QTL clusters (all except C6), were found to be highly homologous with 282 high confidence (HC) and 57 low confidence (LC) candidate genes based on IWGSC RefSeq v 1.0. These stable QTLs and RHF-QTLs, especially those grouped into clusters, are credible and should be given priority for QTL fine-mapping and identification of candidate genes with which to explain the molecular mechanisms of quality development and inform marker-assisted breeding in the future.
Identification of Speech Characteristics to Distinguish Human Personality of Introversive and Extroversive Male Groups

According to the similarity-attraction theory, humans respond more positively to people who are similar in personality. This observation also holds true between humans and robots, as shown by recent studies that examined human-robot interactions. Thus, it would be conducive for robots to be able to capture the user personality and adjust the interactional patterns accordingly. The present study is intended to identify significant speech characteristics such as sound and lexical features between the two different personality groups (introverts vs. extroverts), so that a robot can distinguish a user’s personality by observing specific speech characteristics. Twenty-four male participants took the Myers-Briggs Type Indicator (MBTI) test for personality screening. The speech data of those participants (identified as 12 introvertive males and 12 extroversive males through the MBTI test) were recorded while they were verbally responding to the eight Walk-in-the-Wood questions. After that, speech, sound, and lexical features were extracted. Averaged reaction time (1.200 s for introversive and 0.762 s for extroversive; p = 0.01) and total reaction time (9.39 s for introversive and 6.10 s for extroversive; p = 0.008) showed significant differences between the two groups. However, averaged pitch frequency, sound power, and lexical features did not show significant differences between the two groups. A binary logistic regression developed to classify two different personalities showed 70.8% of classification accuracy. Significant speech features between introversive and extroversive individuals have been identified, and a personality classification model has been developed. The identified features would be applicable for designing or programming a social robot to promote human-robot interaction by matching the robot’s behaviors toward a user’s personality estimated.
Pushing the envelope: Micro-transmitter effects on small juvenile Chinook salmon (Oncorhynchus tshawytscha)

Significant effort has been invested in downsizing telemetry transmitters so they can be used to monitor survival and behavior in a variety of fish species and life stages. Commercially available "micro" transmitters in particular have presented researchers with the opportunity to tag very small fish (< 250 mm fork length). We conducted a release/recapture study in tandem with a laboratory study of tag effects on juvenile yearling spring and subyearling fall Chinook salmon (Oncorhynchus tshawytscha). Fish surgically implanted with both a micro-acoustic transmitter and passive integrated transponder (PIT) tags were compared with fish injected with only a PIT tag. Detections from both tag types showed that during the downstream migration, fish surgically implanted with both a micro-acoustic transmitter and PIT tag did not survive at the same rate or behave in the same manner as those injected with only a PIT tag. Differences in survival were more pronounced in subyearlings than in yearlings. This was likely due to warmer temperatures experienced by migrating subyearlings, their higher metabolic rate, and their smaller size and consequently higher tag-burden. To identify the mechanisms driving these differences, we necropsied migrating study fish recaptured at locations 225-460 km downstream from the release site. Results revealed that compared with PIT-tagged fish, micro-acoustic-tagged fish had heightened inflammatory responses within the body cavity, delayed healing of surgical incision sites, and poor body-condition. For study fish tagged along with those released to the river but held in the laboratory for observation, outcomes revealed that tag effects were similar in direction, but not as pronounced under artificial conditions.