Miracle-dz/UniFAR-FTUs huggingface dataset:
The complete mitochondrial genome of Pseudocrangonyx daejeonensis (Crustacea: Amphipoda: Pseudocrangonyctidae)

Abstract The complete mitogenome sequence of a subterranean pseudocrangonyctid amphipod, Pseudocrangonyx daejeonensis, was determined. The complete mitogenome of P. daejeonensis was 15,069 bp in length with the typical 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a control region (CR). This is the first complete mitogenome sequence in the family Pseudocrangonyctidae. Interestingly, gene arrangements of most amphipod species were almost identical to the typical pan-crustacean ground pattern, whereas two PCGs, both of rRNAs and CR were translocated in P. daejeonensis. A maximum-likelihood tree, constructed based on 30 eumalacostracan mitogenomes, confirmed that P. daejeonensis is closely related to the crangonyctid Stygobromus indentatus and S. tenuis potomacus and supported the monophyly of the superfamily Crangonyctoidea.
Regulation of Immunity to Visceral Leishmaniasis by Regulatory T cells (Tregs) and Hepatic Stellate Cells (HSCs)

Leishmaniasis is a vector borne disease that spreads through the bite of an infected sandfly and is caused by the intracellular parasite-Leishmania. An estimated 10-15 million cases of leishmaniasis occur worldwide, presenting as one of the three forms: cutaneous (CL), mucocutaneous (MCL) or visceral leishmaniasis (VL). The occurrence of leishmaniasis is increasing due to global traveling, emergence of drug resistant species and Leishmania-HIV coinfection. Therefore, there is an urgent need for the development of new therapies or vaccines against leishmaniasis. Our laboratory previously showed that mice with an inactivating knock-in mutation in the p110δ gene (known as p110δ) are resistant to L. major (the causative agent of CL). Here, I demonstrate that signaling via the p110δ also regulates immunity to L. donovani (the causative agent of VL) resulting in hyper-resistance to experimental VL. This outcome is dependent on the impact of p110δ signaling on expansion and function of regulatory T cells (Tregs). I show for the first time that L. donovani can infect Hepatic Stellate Cells (HSCs) in vivo and in vitro and this infection leads to the production of cytokines that are known to induce Tregs. I also demonstrate that infection with L. donovani leads to dramatic expansion of HSCs in a PI3K-dependent manner, and this correlates with expansion of hepatic Tregs. I further show that L. donovaniinfected HSCs can induce CD4 T cells to become Tregs and this effect is dependent on p110δ signalling. Targeted depletion of HSCs during infection caused a dramatic reduction in liver Treg numbers and proliferation, which was associated with a more efficient parasite control. I also demonstrate that prophylactic and therapeutic administration of CAL-101 (a pharmacological
EU start-up calculator: impact of COVID-19 on aggregate employment: Scenario analysis for Austria, Belgium, Germany, Hungary, Italy and Spain

Early data show that the COVID-19 pandemic has affected particularly strongly start-up activity. This may have dramatic and lasting effects on aggregate employment which persist as the cohort of new firms age. To assess the impact, we developed the EU start-up calculator. This is an empirical tool that allows to conduct scenario analysis to compute the impact that the disruption of start-up activity has on aggregate employment on EU Member States and their economic sectors. In particular, we simulate a strong (i.e. of magnitude equivalent to the Great Recession) but short-lived (i.e. lasting one-year) crisis in Austria, Belgium, Germany, Hungary, Italy and Spain. This shock generates important and persistent job losses in all the countries that range between 0.7 (Belgium) to 2.2% (Austria) in 2020 and adds to a cumulative employment loss for the period 2020-2030 that ranges between 82 thousand (Belgium) to 1186 thousand (Italy). The negative impact is particularly high in Austria, Hungary, Italy and Spain, as well as in the service sector, which are characterized by a high firm turnover and that rely on start-ups and young firms for job creation. We also find that in most countries the deterioration of the survival rate of young firms plays an important role in driving employment, seconded by the number of new entrants. As a consequence, policies aimed at supporting young firms and incentivizing the creation of new ones may significantly mitigate the medium-term effect of the pandemic. In fact, when we simulate bounce-back scenarios where the number of firms entering the economy rapidly increases in 2021, in every country the outlook is significantly improved, the recovery is faster and the aggregate job loss is lower.
Essential Host Factors for Human Parvovirus B19 Replication

Parvovirus B19 (B19V) is a small, non-enveloped virus that contains a single-stranded DNA (ssDNA) genome of 5.6 kb in size. B19V is pathogenic to humans and causes bone marrow failure diseases and various other inflammatory disorders. B19V infection exhibits high tropism for human erythroid progenitor cells (EPCs) in the bone marrow and fetal liver. The exclusive restriction of B19V replication to erythroid lineage cells is partly due to the expression of receptor and co-receptor(s) on the cell surface of human EPCs and partly depends on the intracellular factors essential for virus replication. On this rationale, we tried to investigate the essential host factors for efficient virus replication. Our results demonstrated that signal transducer and activator of transcription 5 (STAT5) and RNA-binding motif protein-38 (RBM38) are two of these important host factors that ensure virus DNA replication and premRNA processing, respectively, during B19V infection. The stages of erythropoiesis during which STAT5 is activated and RBM38 is expressed, are highly susceptible to B19V infection, thus suggesting that these two factors are among the key determinants of the B19V restriction to human EPCs. B19V requires erythropoietin (EPO) signaling and hypoxia for its efficient replication. EPO to EPO receptor signaling activates JAK2-STAT5 pathway that phosphorylates STAT5. In our first study, we show that phosphorylated STAT5 is critical for B19V replication. Upon insilico analysis, we identified a consensus STAT5 binding element adjacent to the NS1-binding elements within the minimal origin of viral DNA replication (Ori) in the B19V genome. The phosphorylated STAT5 specifically interacts with viral Ori both in vivo and in vitro, and is actively recruited within the viral DNA replication centers. Furthermore, our study shows a novel interaction between STAT5 and the minichromosome maintenance (MCM) complex. Our
Initial growth of Brazilian Firetree (Schizolobium parahyba (Vell.) S.F.Blake) fertilized with phosphorus in Red-Yellow Latosol

Due to the scarcity of information on the use of fertilizers on the Brazilian Firetree (locally known as guapuruvu), this study aimed to evaluate the initial stages of guapuruvu (Schizolobium parahyba (Vell.) S.F. Blake. ) plant growth using different doses of phosphorus (P). The experiment was conducted within a greenhouse environment in dystrophic Red-Yellow Latosol soil samples from the Brazilian Cerrado as the substrate in plastic containers with a capacity of 10 dm 3 . The experimental design was completely randomized, with six treatments and four replicates each, totaling 24 experimental units. The treatments consisted of six different treatments of P: 0; 50; 100; 150; 200 and 250 mg dm -3 , using triple superphosphate as the P-source. At 150 days, the plant height, root crown diameter, number of leaves, dry matter (leaves, stems and roots) and P content in leaves, stems, roots and soil were evaluated. In addition, the Dickson Quality Index was estimated. The results showed that plants treated with doses of 100 to 250 mg dm -3 of P had higher growth rates among the analyzed variables. Guapuruvu revealed a requirement for phosphorus in the early stages of development at the studied edaphoclimatic condition, demonstrating the necessity for the application of phosphorus doses above 100 mg dm -3 via fertilizers in soils with low P availability.
The complete mitochondrial genome of a Pabdah catfish, Ompok pabda (Hamilton, 1822)

Abstract The complete mitochondrial genome of Pabdah catfish, Ompok pabda (Hamilton, 1822) was determined by MiSeq platform. The genome was 16,512 bp in length, in which canonical 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and a control region (D-Loop) were encoded. The overall A + T (55.10%) content was higher than G + C (44.90%). Total nine genes were located at the L strand, and remaining 28 genes were at the H strand. The start codon of 12 protein-coding genes was ATG, except for COX1 (GTG). Incomplete stop codon (TA−/T–) was identified in six genes including COXII, COXIII, ND2, ND3, ND4, and Cytb. The mitogenome of O. pabda showed highest sequence identity with O. bimaculatus (86%).
Gas exchange and chlorophyll content in tomato grown under different organic fertilizers and biofertilizer doses

The correct management of organic fertilization has been shown as an extremely viable alternative in the production of vegetables, providing high yields concomitant to the reduction of synthetic fertilizers. The improvement of the production can be interpreted by the physiological behavior, favored by the organic fertilization with the supply of nutrients. Thus, an experiment was carried out to evaluate the gas exchange and Soil-Plant Analyses Development (SPAD) chlorophyll content in tomato plants according to types of organic fertilizers and biofertilizer doses. The experimental design was completely randomized with treatments distributed in factorial arrangement (3 x 5), referring to organic fertilizer types (T1: earthworm humus; T2: goat manure and T3: cattle manure) and biofertilizer concentrations (600, 800, 1000, 1200 and 1400 ml), with four replications. The gas exchange and the SPAD chlorophyll content in tomato plants depend on the type of organic fertilizer and the concentration of biofertilizer. It was possible to observe that increasing doses of biofertilizer in the substrate with low organic concentration increase the gas exchange in tomato plants, while high doses together with more concentrated organic fertilizers reduce these characteristics. Key words: Alternative fertilizer, Lycopersicon esculentum, photosynthesis, organic fertilizer.
IncGraph: An Improved Distributed Incremental Graph Computing Model and Framework Based on Spark GraphX

The excavated information will become obsolete when the data changes in dynamic graphs. To compute the up-to-date results, the graph algorithm has to re-compute the entire data from scratch, which will consume huge computation time and resources. To reduce the cost of such calculations, this paper proposes a model called IncGraph to support incremental iterative computation over dynamic graphs. Different from the way of traditional iteration, IncGraph executes the graph algorithm through reusing the results of the previous graph and performs computation on the part of the graph that has changed. IncGraph has two critical components: (1) an incremental iterative computation model that consists of two steps: an incremental step to calculate the results on the changed vertices of the graph, and a merge step to calculate the results on the entire graph by using the results of the previous graph and the incremental step; and (2) an incremental update method to accelerate the iterative process within the iterative graph algorithm. We implement IncGraph model on GraphX and evaluate its performance by using several representative iterative graph algorithms: PageRank, Connected components, and Single Source Shortest Path. The results show that compared with the traditional iteration, when adding the 100k of vertices in different size data sets, the performance optimization ratio of IncGraph is 31.79 percent averagely, and 50.2 percent maximum; and when the percentage of added vertices varied from 0.01 to 10 percent in different data sets, the performance optimization ratio of IncGraph varied from 19.9 to 66.1 percent. Moreover, the result errors of IncGraph is small and can be neglected.
Integrating equality in evaluation of water access for irrigation in an Andean community

Las estadisticas de desarrollo de Bolivia reflejan considerables diferencias entre grupos sociales y geograficos, por ejemplo, la tasa de pobreza es mucho mayor en comunidades rurales agricolas, donde las condiciones de vida dependen en gran medida del acceso a recursos hidricos. Las politicas de intervencion que ignoran el principio de igualdad, pueden en muchos casos agudizar estas diferencias. En este estudio, evaluamos el acceso al agua para riego en una comunidad Andina, integrando una perspectiva de igualdad basada en la desagregacion de datos. Mediante el analisis de datos disponibles, se identifico que la tenencia de derechos de agua y prioridad de distribucion son los criterios principales que controlan el acceso al agua en la comunidad. Segun estos criterios, la demanda de agua fue desagregada en 28 grupos y todo el sistema fue simulado en el software Water Evaluation and Planning (WEAP), con el que se evaluo la cobertura de demanda de agua. Algunos grupos tuvieron una cobertura de demanda de agua menor al 20%, mientras que otros alcanzaron una cobertura cercana al 100%. Estas diferencias no se pueden observar en el modelo agregado del sistema, para el cual la cobertura de demanda de agua fue superior al 60% todo el tiempo. La evaluacion de escenarios de cambio climatico indico que la cobertura de demanda de agua puede reducir en un 15% durante la estacion seca. Sin embargo, la implementacion de estrategias planificadas podria contrarrestar esta reduccion, a traves del incremento de provision e infraestructura de almacenamiento. La implementacion de estas estrategias en el modelo indico que la cobertura de demanda de agua puede aumentar hasta 80% para algunos grupos; sin embargo, otros grupos aun enfrentan escasez. Este estudio hace enfasis en metodos y herramientas de planificacion que pueden reforzar los enfoques de igualdad existentes, y aumentar la eficiencia en la reduccion de pobreza y desigualdad a traves del manejo de agua.
Should I Stay or Should I Go? Intergenerational Transfers and Residential Choice: Evidence from China

We study the impact of parental transfers to their children in early life on the child’s support to the parents at older ages either in time or with money. We conjecture that the type of transfer from the parents has an impact on what kind of help they receive from their children. Using data from the China Health and Retirement Longitudinal Study (CHARLS) we ﬁnd that transfers in children’s education are associated with higher ﬁnancial help at older ages. In contrast, transfers to support the children’s marriage are positively linked with time support, such as more visits, from children to their parents. The children’s residential decisions are identiﬁed as an important mechanism: transfers into education tend to let children move further away whereas marital transfers are associated with children staying closer to one’s parents.
Performance and digestive characteristics in dairy cattle maintained under grazing regime and supplemented with linseed

Linseed is characterized by the rich unsaturated fatty acids and slow release of oil in the rumen. The objective of the present study was to evaluate the intake, rumen degradability, and digestibility of dairy cattle maintained under grazing regime and supplemented with linseed. Twelve Girolando (¾ Holstein × ¼ Gir) cows with a mean age of 5.2 ± 1.9 years and body weight of 583.9 ± 67.6 kg were used. The cows were maintained in a rotational grazing system on the mombaça grass (Panicum maximum) and divided into 2 experimental groups. The CONTR (n = 6) group received only the base diet (pasture) and the LINS group (n = 6) received pasture plus linseed. Initially, a fifteen-day adaptation period was established to stabilize the desired consumption amount. After this period, the animals received 800 g of linseed for 126 d. The ruminal degradability was evaluated using 2 males with a cannula in the rumen, and they were provided the same experimental diet. The measured dry matter production of pasture did not show differences during the experimental period. The intake of crude protein and ethereal extract was higher, whereas neutral detergent fiber content was lower in the cows supplemented with linseed. However, this did not alter the weight gain and digestibility coefficients, except the digestibility coefficient of the ethereal extract. At the ruminal level, linseed showed low effective and potential degradability with negative effect on grass degradability.
Determination of the Mutagenicity of 2-aminoanthracene Using Chicken Hepatic S-9 Fraction

The abundance of potential genotoxins makes it necessary to improve microbial test systems that allow to identify these substances quickly. At the same time, the methodology of metabolic activation of promutagens in vitro is very important. Microsomal preparations of rodents, which are generally used for that, have significant disadvantages associated with the potential health risks of the inducers of the metabolism of microsomal cytochromes. For the metabolic activation of promutagens, we have developed a protocol of microsomal homogenate fraction (S-9) preparation. We propose to complement the set of methods for quality control of feeds for valuable and rare chicken breeds with the Ames test (Salmonella/microsome) using the activated chicken hepatic S-9 homogenate fraction.
A Data-Driven Health Monitoring Method Using Multiobjective Optimization and Stacked Autoencoder Based Health Indicator

This article proposes a new data-driven health monitoring method, which uses multiobjective optimization and stacked autoencoder based health indicator. Specifically, the proposed method proposes an improved nondominated sorting genetic algorithm-II (NSGA-II) to perform multiobjective optimization on a large number of candidate features extracted from the sensor measurements. Then, a stacked autoencoder model is used to construct health indicators from the selected features. In the improved NSGA-II algorithm, the optimization goals of feature selection are defined as the minimum gap of health indicators between different states and the number of features. Comparisons between the proposed method and the state-of-the-art methods on simulation experiments show that the proposed method can accurately identify the status of the equipment and effectively limit the complexity of the diagnostic model.
CLASSIFICATION OF CUSTOMER COMPLAINTS ON INSTAGRAM COMMENTS USING NAÏVE BAYES ALGORITHM WITH N-GRAM FEATURE EXTENSION

Customer complaints about the company can be used as a form of self-evaluation and performance that has been carried out by the company, based on customer complaints the company can find out the weaknesses that exist in the company and fix them. The forms of submitting customer complaints are very diverse, currently not only by telephone, but customers also submit suggestions or complaints, customers can submit suggestions or complaints via electronic mail or email or forums in cyberspace that are indeed created by product-producing companies to accommodate various complaints, suggestions, and direct criticism from consumers, especially social media that are free to express opinions on the delivery services used. Instagram is a social media that is more inclined towards images and on the other hand, has captions and comments text, a study is needed for the problem of customer complaints from shipping service users on an Instagram account of a delivery service company. Based on this background, a solution is needed in solving problems for text mining classification using Naïve Bayes with SMOTE techniques and N-Gram feature extraction with the usual process for text mining so that it can produce Naïve Bayes and SMOTE accuracy with an accuracy of 88.54%, before implementation. N-Gram and the accuracy rate increased by 1.44% after the N-Gram Term was applied to 89.98% by using a dataset of 776 Instagram comment text records that had to preprocess text.
Symmetry breaking and inter-clonal behavioural variability in a slime mould

Cells are dynamic systems capable of switching from isotropic growth to polarized growth even in the absence of any pre-existing external asymmetry. Here, we study this process of symmetry breaking in the acellular slime mould Physarum polycephalum. In these experiments, slime moulds could grow on two identical opposed sources of calcium. We highlighted a positive correlation between growth dynamic, level of symmetry breaking and calcium concentration. We identified three populations of slime moulds within our clonal lineage with similar symmetry breaking behaviours but different motility characteristics. These behavioural differences between slime moulds emerged in the absence of any environmental differences. Such behavioural plasticity could generate cellular diversity, which can be critical for survival.
Effective Approaches in Reducing Reading Discrepancy Scores between Students in General Education and Special Education

This research study examined curricular and instructional approaches that help students who receive special education services meet common Illinois state standards as measured by annual state standardized testing. Despite having supportive accommodations and modifications, Illinois students who receive special education services have lagged behind their general education peers in meeting academic standards as measured by annual Illinois state testing. Participants included personnel from schools that were identified as being high performing while also having the smallest discrepancy between students in general education and special education. These schools were investigated to determine what approaches they use to have this reduced achievement gap. Teachers and administrators from these schools were interviewed to gain insights regarding effective instructional and curricular methods. The results suggest that schools closing the achievement gap implemented instructional approaches including co-taught and inclusion classrooms, differentiation, and time for professional planning and collaboration. Additionally, these schools offered purchased, researched-based reading curricula that were implemented with high fidelity. Teachers in both special education and general education had the same materials to reference and offer students. Implications for practice and future research directions are reported.