Miracle-dz/UniFAR-FTUs huggingface dataset:
Induction of ALP and MMP9 activity facilitates invasive behavior in heterogeneous human BMSC and HNSCC 3D spheroids

Mesenchymal stem cells (MSCs) are multipotent progenitor cells capable of differentiating into adipocytic, osteogenic, chondrogenic, and myogenic lineages. There is growing evidence that MSCs home into the tumor microenvironment attracted by a variety of signals such as chemokines, growth factors, and cytokines. Tumor‐homing stem cells may originate from bone marrow–derived MSCs (BMSCs) or adipose tissue‐derived MSCs. Recent scientific data suggest that MSCs in combination with tumor cells can either promote or inhibit tumorigenic behavior. In head and neck squamous cell carcinoma (HNSCC), BMSCs are reported to be enriched with a potential negative role. Here, we evaluated the effect of BMSCs from 4 different donors in combination with 4 HNSCC cell lines in a 3‐dimensional multicellular spheroid model. Heterogeneous combinations revealed an up‐regulation of gene and protein expression of osteogenic markers runt‐related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) together with a substantial secretion of matrix metalloproteinase 9. Moreover, heterogenous BMSC/tumor spheroids showed increased invasion compared with homogenous spheroids in a Boyden chamber invasion assay. Furthermore, inhibition of ALP resulted in a substantially decreased spreading of heterogeneous spheroids on laminin‐rich matrix. In summary, our data suggest a prometastatic effect of BMSCs combined with HNSCC. —Wessely, A. , Waltera, A. , Reichert, T. E. , Stöckl, S. , Grässel, S. , Bauer, R. J. Induction of ALP and MMP9 activity facilitates invasive behavior in heterogeneous human BMSC and HNSCC 3D‐spheroids. FASEB J. 33, 11884–11893 (2019). www.fasebj. org
Research on Multi-level Inverter of Multi-terminal Switch Network and Extension Topology

Due to multilevel inverters can have more levels of output voltage at the output waveform, closer to sine waves, and with small output waveform THD, and low voltage stress, low system advantages of EMI, multilevel inverters are widely recognized, and get a certain application. Based on the analysis of the characteristics of the basic multi-level inverter topology and the scope of application, this paper explores the idea and method of multi-level inverter topology using multi-terminal switch network. By comparing the new topology and basic topology, it can be seen that this new multi-level inverter topology is easy to expand, requiring small filter inductance volume and inductance, loss and low cost, which is an important direction for the development of topological circuits. The new multi-level topology has broadened the research scope of multi-level inverter topology and created new ideas and directions for multi-level topology research, which provides theoretical guidance for further scientific research and engineering applications. Keywords-multi-level inverter; power topology; multi-port switch network
Delta‐like 3 localizes to neuroendocrine cells and plays a pivotal role in gastrointestinal neuroendocrine malignancy

Delta‐like 3 (DLL3) is a member of the Delta/Serrate/Lag2 (DSL) group of Notch receptor ligands. Five DSL ligands are known in mammals, among which DLL3 has a unique structure. In the last few years, DLL3 has attracted attention as a novel molecular targeting gene in neuroendocrine carcinoma of the lung due to its high expression. However, the expression pattern and functions of DLL3 in the gastrointestinal tract and gastrointestinal neuroendocrine carcinoma remain unclear. In this study, we examined the expression and role of DLL3 in the gastrointestinal tract, as well as in gastrointestinal neuroendocrine carcinoma. Immunohistochemical staining of the human normal gastrointestinal tract revealed that DLL3 localized in neuroendocrine cells. DLL3 showed intense staining in chromogranin A‐positive gastric cancer specimens. Real‐time quantitative RT‐PCR and western blotting analyses showed considerable upregulation of DLL3 in gastrointestinal neuroendocrine carcinoma cell lines. Immuno‐electron microscopy demonstrated abundant expression of DLL3 in neurosecretory granules in these cells. Furthermore, gene silencing of DLL3 caused significant growth inhibition through the induction of intrinsic apoptosis. Our findings suggest that DLL3 is expressed in neuroendocrine cells of the gastrointestinal tract and that it has a pivotal role in gastrointestinal neuroendocrine carcinoma cells. Based on these findings, further investigations are required to achieve a breakthrough in developing therapeutic strategies for gastrointestinal neuroendocrine carcinoma.
Expressive and efficient streaming libraries

Οι ροές επεξεργασίας δεδομένων είναι και πάλι στο προσκήνιο: ευρέως-διαδεδομένες βιβλιοθήκες για επεξεργασία ροών υπάρχουν σε κάθε μοντέρνα γλώσσα προγραμματισμού και οικοσύστημα, από την Java στην C# και από την Scala και OCaml στη Haskell. Ωστόσο, καίρια χαρακτηριστικά τους όπως η εκφραστικότητα και οι επιδόσεις έχουν σημαντικά περιθώρια βελτίωσης. Στην παρούσα διατριβή, εξακριβώνουμε το ποια είναι ακριβώς τα ποιοτικά χαρακτηριστικά κάθε βιβλιοθήκης και παρατηρούμε ότι παρούσες αλλά και μελλοντικές χρήσεις τους, είναι άρρηκτα συνδεδεμένες (και δεσμευτικές) με την εκάστοτε υλοποίηση. Απλοί μηχανισμοί αφαίρεσης δεν είναι επαρκείς. Είναι εφικτό να διαμορφώσουμε την υλοποίηση των βιβλιοθηκών ροών και να τις κάνουμε πιο επεκτάσιμες και με μεγαλύτερες επιδόσεις; Παρουσιάζουμε δυο λύσεις προς την επίτευξη της επεκτασιμότητάς τους. Κατά την πρώτη, διαχωρίζουμε τους ορισμούς του συντακτικού και της σημασιολογίας των βιβλιοθηκών και κατά τη δεύτερη λύση απελευθερώνουμε τις εν λόγω βιβλιοθήκες από την επιτακτική ανάγκη ύπαρξης ενός ικανού και έξυπνου μεταγλωττιστή. Ο στόχος της διατριβής είναι να δημιουργήσει επεκτάσιμες βιβλιοθήκες ροών, υψηλών επιδόσεων, διατηρώντας όμως την υψηλού επιπέδου δομή τους. Η παρουσίαση των ερευνητικών μας αποτελεσμάτων έπεται αξιολόγησης επιδόσεων σε σύγχρονες βιβλιοθήκες που διεξήγαμε για διάφορες σύγχρονες γλώσσες προγραμματισμού. Στη συνέχεια προτείνουμε έναν μηχανισμό για την ενίσχυση της συντήρησης βιβλιοθηκών ροών διατηρώντας ενός υψηλού επιπέδου επεκτασιμότητα. Για να το επιτύχουμε αυτό, θεωρούμε τις εν λόγω βιβλιοθήκες ως γλώσσες προγραμματισμού ειδικού σκοπού, σχεδιάζοντας και υλοποίώντας την βιβλιοθήκη StreamAlg, μία βιβλιοθήκη που προσφέρει την δυνατότητα να δεχθεί επεκτάσεις με νέες μεθόδους και συμπεριφορά κατ' επιλογή. Στη συνέχεια, παρουσιάζουμε την εφαρμογή της προαναφερθείσας τεχνικής σε μία γλώσσα προγραμματισμού, την Java, για να επιτύχουμε την ίδια γενικότητα κατά την επέκταση της, με το εργαλείο που ονομάζουμε Recaf. Δείχνουμε πώς δημιουργούμε διαλέκτους στην Java για να υποστηρίξουμε νέο συντακτικό και νέα σημασιολογικά στοιχεία στην ίδια τη γλώσσα. Ως αποτέλεσμα, δημιουργήσαμε επεκτάσεις της Java, μία εκ των οποίων μας έδωσε τη δυνατότητα να δημιουργήσουμε βιβλιοθήκη ροών στη Java, όπως ακριβώς γίνεται και στην C#. Τέλος παρουσιάζουμε την πρότασή μας για βιβλιοθήκες ροών υψηλών επιδόσεων που υλοποιήσαμε σε δύο γλώσσες προγραμματισμού με τον ενιαίο όρο-ομπρέλα Strymonas. Η βιβλιοθήκη που παρέχουμε, χωρίς να βασίζεται σε απροσπέλαστους βελτιστοποιητές ή ``ικανοποιητικά-ικανούς'' μεταγλωττιστές, παρέχει, υψηλού επιπέδου, εγγυημένη και φορητή ταχύτητα ως προς την επεξεργασία δεδομένων. Η προσέγγισή μας βασίζεται σε έννοιες υψηλού επιπέδου οι οποίες στη συνέχεια υλοποιούνται.
Crack initiation and propagation in sweet cherry skin: A simple chain reaction causes the crack to ‘run’

Rain cracking severely affects the commercial production of many fleshy-fruit species, including of sweet cherries. The objectives were to investigate how the gaping macroscopic cracks (macrocracks) of a rain-cracked fruit can develop from microscopic cracks in the cuticle (microcracks). Incubating fruit in deionized water is well known to cause significant macrocracking. We found that after a lag phase of 2 h, the numbers and lengths of macrocracks increased. Macrocrack number approached an asymptote at 12 h, whereas macrocrack length continued to increase. The rate of macrocrack propagation (extension at the crack tip) was initially 10.8 mm h-1 but then decreased to a near-constant 0.5 mm h-1. Light microscopy revealed three characteristic zones along a developing macrocrack. In zone I (ahead of the crack), the cuticle was intact, the epidermal cells were unbroken and their cell walls were thin. In zone II, the cuticle was fractured, the first epidermal cells died and their cell walls began to thicken (swell). In zone III, most epidermal cells had died, their cell walls were swollen and cell:cell separation began along the middle lamellae. The thickness of the anticlinal epidermal cell walls and the percentage of intact living cells along a crack were closely and negatively related. Cracks were stained by calcofluor white, but there was no binding of monoclonal antibodies (mAbs) specific for hemicelluloses (LM11, LM21, LM25). Strong binding was obtained with the anti-homogalacturonan mAb (LM19), indicating the presence of unesterified homogalacturonans on the crack surface. We conclude that macrocrack propagation is related to cell death and to cell wall swelling. Cell wall swelling weakens the cell:cell adhesion between neighbouring epidermal cells, which separate along their middle lamellae. The skin macrocrack propagates like a ‘run’ in a fine, knitted fabric.
Antibiotic-Resistant Septicemia in Pediatric Oncology Patients Associated with Post-Therapeutic Neutropenic Fever

Death in cancer patients can be caused by the progression of tumors, their malignity, or other associated conditions such as sepsis, which is a multiphasic host response to a pathogen that can be significantly amplified by endogenous factors. Its incidence is continuously rising, which reflects the increasing number of sick patients at a higher risk of infection, especially those that are elderly, pediatric, or immunosuppressed. Sepsis appears to be directly associated with oncological treatment and fatal septic shock. Patients with a cancer diagnosis face a much higher risk of infections after being immunosuppressed by chemotherapy, radiotherapy, or anti-inflammatory therapy, especially caused by non-pathogenic, Gram-negative, and multidrug-resistant pathogens. There is a notorious difference between the incidence and mortality rates related to sepsis in pediatric oncologic patients between developed and developing countries: they are much higher in developing countries, where investment for diagnosis and treatment resources, infrastructure, medical specialists, cancer-related control programs, and post-therapeutic care is insufficient. This situation not only limits but also reduces the life expectancy of treated pediatric oncologic patients, and demands higher costs from the healthcare systems. Therefore, efforts must aim to limit the progression of sepsis conditions, applying the most recommended therapeutic regimens as soon as the initial risk factors are clinically evident—or even before they are, as when taking advantage of machine learning prediction systems to analyze data.
Helminth parasites in two populations of Astronotus ocellatus (Cichliformes: Cichlidae) from the eastern Amazon, Northern Brazil.

This study compared the structure of helminth parasite communities in two populations of Astronotus ocellatus from two localities in Pará State, eastern Amazon (Brazil). Hosts from the Tapajós River were infected by Posthodiplostomum sp. metacercarie, larvae of Contracaecum sp. and Pseudoproleptus sp. , with predominance of Contracaecum sp. Hosts from Iara Lake were infected by Procamallanus spiculastriatus, Proteocephalus sp. and Acanthocephala gen. sp. , with predominance of P. spiculastriatus. Parasites had an aggregated dispersion and there were differences in Shannon diversity index and the evenness between both host populations, because the parasite component community showed no similarity. The existence of variation in infracommunity and community of parasites for A. ocellatus from different localities indicates the presence of an uneven distribution in terms of species and density of parasites, as well as of intermediate hosts in the localities surveyed.
Depression, disability and sleep disturbance are the main explanatory factors of fatigue in rheumatoid arthritis: a path analysis model.

OBJECTIVES Fatigue is one of the most prevalent and disabling symptoms among patients with rheumatoid arthritis (RA), however, it is frequently neglected by health professionals. This study aimed to develop a multidimensional explanatory model of fatigue in patients with RA as a basis for better understanding and intervention. METHODS This was an ancillary analysis of an observational, cross-sectional, single centre study. Patients completed a questionnaire including demographic data and measures of pain, sleep, disability, anxiety, depression, and personality. Fatigue was assessed by the Functional Assessment of Chronic Illness Therapy - Fatigue (FACIT-F). Disease activity and haemoglobin levels were assessed. Path analysis was performed to test and improve a hypothesised model for fatigue. RESULTS This analysis included 142 patients, with a mean (SD) age of 61.1 (11.7) years. The final path analysis model presented acceptable fit and explained 60.0% of the variance of fatigue. The predominant direct explanatory factors identified were disability (46.5%) and depression (41.2%), the latter having an additional indirect influence of 19% through disability. Age (-16.2%) and sleep disturbance (15.7%) were also directly linked to fatigue. Personality trait extroversion (-22.4%), pain (20.0%), and disease activity (14.9%) are only indirectly related to fatigue. CONCLUSIONS Depression, disability and sleep disturbance appear to be the main factors explaining fatigue in patients with RA. Disease activity, pain, and personality seem to play only a secondary role, extroversion being the only personality trait associated with fatigue. These findings foster a shift in the paradigm of care towards a more holistic management of fatigue, integrating adjunctive therapies beyond measures targeted solely at disease remission.
A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed (Brassica napus L.) germplasm

Summary Silique number is the most important component of yield in rapeseed (Brassica napus L.). To dissect the mechanism underlying the natural variation of silique number in rapeseed germplasm, a series of studies were performed. A panel of 331 core lines was employed to genome‐wide association study (GWAS), and 27 loci (including 20 novel loci) were identified. The silique number difference between the more‐ and fewer‐silique lines can be attributed to the accumulative differences in flower number and silique setting rate. Each of them accounted for 75.2% and 24.8%, respectively. The silique number was highly associated with the total photosynthesis and biomass. Microscopic analysis showed that the difference between extremely more‐ and fewer‐silique lines normally occurred at the amount of flower bud but not morphology. Transcriptome analysis of shoot apical meristem (SAM) suggested that most of enriched groups were associated with the auxin biosynthesis/metabolism, vegetative growth and nutrition/energy accumulation. By integrating GWAS and RNA‐seq results, six promising candidate genes were identified, and some of them were related to biomass accumulation. In conclusion, the natural variation of silique number is largely affected by the biomass and nutrition accumulation, which essentially reflects the positive regulatory relationship between the source and sink. Our study provides a comprehensive and systematic explanation for natural variation of silique number in rapeseed, which provides a foundation for its improvement.
Shared Pathogenomic Patterns Characterize a New Phylotype, Revealing Transition toward Host-Adaptation Long before Speciation of Mycobacterium tuberculosis

Abstract Tuberculosis remains one of the deadliest infectious diseases of humanity. To better understand the evolutionary history of host-adaptation of tubercle bacilli (MTB), we sought for mycobacterial species that were more closely related to MTB than the previously used comparator species Mycobacterium marinum and Mycobacterium kansasii. Our phylogenomic approach revealed some recently sequenced opportunistic mycobacterial pathogens, Mycobacterium decipiens, Mycobacterium lacus, Mycobacterium riyadhense, and Mycobacterium shinjukuense, to constitute a common clade with MTB, hereafter called MTB-associated phylotype (MTBAP), from which MTB have emerged. Multivariate and clustering analyses of genomic functional content revealed that the MTBAP lineage forms a clearly distinct cluster of species that share common genomic characteristics, such as loss of core genes, shift in dN/dS ratios, and massive expansion of toxin–antitoxin systems. Consistently, analysis of predicted horizontal gene transfer regions suggests that putative functions acquired by MTBAP members were markedly associated with changes in microbial ecology, for example adaption to intracellular stress resistance. Our study thus considerably deepens our view on MTB evolutionary history, unveiling a decisive shift that promoted conversion to host-adaptation among ancestral founders of the MTBAP lineage long before Mycobacterium tuberculosis has adapted to the human host.
Relation between microRNA‐21, transforming growth factor β and response to treatment among chronic hepatitis C patients

Persistence of hepatitis C virus (HCV) infection and response to antiviral therapy has been shown to be associated with inappropriate levels of cytokines and microRNAs (miRNAs). miRNA levels have been reported to fluctuate during treatment. Thus they could be useful predictors for responses to treatment among HCV infected patients, thereby reducing ineffective treatments.
[Analysis on regional homogeneity of resting brain during balance acupuncture-induced analgesic effect in migraine patients without aura].

OBJECTIVE To observe the relationship between the analgesic effect of balance acupuncture and functional changes in brain in patients with migraine without aura. METHODS A total of 40 cases of migraine without aura were equally randomized into a headache-acupoint group and a sham-acupoint group. When acupuncture given, a filiform needle was inserted into the headache-acupoint (the midpoint of the depression region anterior to the juncture of the first and second metatarsal bones on the dorsum of the foot) or the sham point (the midpoint of the depression region anterior to the juncture site between the 3rd and 4th metatarsal joints of the dorsum of the foot) about 25-40 mm deep and manipulated for a while till the patient experienced feelings of electric shock and numbness, then withdrawn immediately. The treatment was conducted once daily for 4 weeks. The visual analogue scale (VAS) was used to evaluate the severity of pain, and the regional homogeneity (ReHo) analysis of resting state functional magnetic resonance imaging (fMRI) was used to assess changes of the spontaneous brain activity. RESULTS After acupuncture, the analgesic effect of headache-acupoint was better than that of the sham-acupoint in both intervention stage and the follow-up stage (P< 0.05), and was also stronger in the intervention stage than in the follow-up stage (P<0.05). There was no significant difference in the analgesic effect between the intervention stage and the follow-up stage in the sham-acupoint group (P>0.05). Compared with pre-intervention, 4-weeks' intervention at the headache-acupoint showed an increase of ReHo values in the anterior cingulate gyrus, anterior central gyrus, superior orbital frontal gyrus, insula, inferior lobule, left anterior cingulate gyrus, ventral lateral nucleus and ventral posteromedial nucleus of the thalamus, pontine nucleus, cerebellar tonsils and orbital frontal inferior gyrus of the brain (P<0.05), and a decrease of ReHo values in the right brain bridge, central posterior gyrus, posterior cingulate gyrus, left central anterior gyrus, posterolateral nucleus of thalamus, and hippocampus (P<0.05), separately. In the sham-acupoint group, the ReHo value was increased in the right tongue gyrus, the left anterior lobe, the anterior cingulate gyrus and the lower occipital gyrus of the brain (P<0.05), and reduced in the left ventral posterolateral nucleus of the thalamus, separately (P<0.05). CONCLUSION Balance acupuncture stimulation of headache acupoint has an analgesic effect in migraine patients without aura, which may be related to its effect in regulating resting state brain function of the limbic-system-dominated multiple brain regions.
Analisis Penggunaan Frequency Band 400 MHz dan 700 MHz untuk Layanan Broadband PPDR di Indonesia

ABSTRAK Layanan komunikasi Public Protection and Disaster Relief (PPDR) di Indonesia saat ini bekerja pada frequency band 400 MHz melalui teknologi narrowband. Semakin beragamnya layanan dan kebutuhan informasi membutuhkan pengembangan ke arah penerapan teknologi broadband. Adanya wacana Analog Switch Off (ASO), memungkinan frequency band 700 MHz dapat dimanfaatkan layanan komunikasi PPDR dengan penerapan teknologi LTE. Melalui kajian penggunaan frequency band 400 MHz dan 700 MHz, layanan broadband PPDR kejadian PP1 membutuhkan lebar band sebesar 10 MHz untuk baik pada arah uplink maupun downlink. Jumlah sel yang diperlukan pada penggunaan frequency band 400 MHz adalah 6482 sel pada tahun 2017 dan meningkat menjadi 6744 sel pada tahun 2021, sedangkan frequency band 700 MHz dari 12901 sel menjadi 13510 sel. Kata kunci: PPDR, broadband, ASO, PP1, LTE ABSTRACT The Public Protection and Disaster Relief (PPDR) communication service in Indonesia is currently working on a 400 MHz frequency band through narrowband technology. Increasingly diverse services and information needs require development towards the application of broadband technology. The discourse of Analog Switch Off (ASO), allows the 700 MHz frequency band can be utilized PPDR communication services with the application of LTE technology. Through the study of the use of 400 MHz and 700 MHz frequency bands, PPDR broadband service incident PP1 requires bandwidth of 10 MHz for both uplink and downlink. The number of cells required on the use of the 400 MHz frequency band is 6482 cells by 2017 and increases to 6744 cells by 2021, while the 700 MHz frequency band from 12901 cells becomes 13510 cells. Keywords: PPDR, broadband, ASO, PP1, LTE
Mindful eating and common diet programs lower body weight similarly: Systematic review and meta‐analysis

Common strategies for reducing body weight rely on limiting energy intake and restricting food choices. However, these strategies have often been proven ineffective in achieving long‐term and sustainable weight reduction. More recently, mindful eating as an alternative weight management strategy has gained increasing attention, yet systematic reviews on intuitive or mindful eating published so far present contradictory results. We performed a systematic review and meta‐analysis on randomized controlled trials on weight loss programs based on mindful or intuitive eating. We analyzed results using meta‐regressions. We included a total of 10 studies and found a significant weight loss effect of mindful/intuitive eating strategies compared with nonintervention controls (−0.348 kg, 95% CI: −0.591 to −0.105, P = 0.005). However, there was no difference compared with conventional diet programs (P = 0.99). Reduction of BMI (−0.137 kg/m2, 95% CI: −0.365 to 0.091, P = 0.240) or waist circumference (−0.358 cm, 95% CI: −0.916 to 0.200, P = 0.209) were not statistically significant. Mindful/intuitive eating could be a practical approach to weight control. Limitations of this study include the unbalanced sex, origin, place of residence of the participants, and the short duration of interventions. Future research should aim at investigating long‐term effects and include a more heterogeneous study population.