Miracle-dz/UniFAR-FTUs huggingface dataset:
Literature-Based Strategies for Clinical Fellow Mentorship

Abstract In the field of communication sciences and disorders, some research and guidance exist on clinical fellowship supervision for the mentor; however, there is a dearth of literature on clinical fellowship supervision from the perspective of the clinical fellow. This article begins with why the clinical fellowship experience is necessary, and then discusses clinical fellow and mentor expectations and responsibilities, clinical fellow independence, mentor qualities, and issues related to the provision of feedback. The overall goal is to enhance clinical fellow mentors' knowledge base on the clinical fellowship experience through review of current literature on supervision and mentorship.
Molecular and Hormonal Regulation of Leaf Morphogenesis in Arabidopsis

Shoot apical meristems (SAM) are tissues that function as a site of continuous organogenesis, which indicates that a small pool of pluripotent stem cells replenishes into lateral organs. The coordination of intercellular and intracellular networks is essential for maintaining SAM structure and size and also leads to patterning and formation of lateral organs. Leaves initiate from the flanks of SAM and then develop into a flattened structure with variable sizes and forms. This process is mainly regulated by the transcriptional regulators and mechanical properties that modulate leaf development. Leaf initiation along with proper orientation is necessary for photosynthesis and thus vital for plant survival. Leaf development is controlled by different components such as hormones, transcription factors, miRNAs, small peptides, and epigenetic marks. Moreover, the adaxial/abaxial cell fate, lamina growth, and shape of margins are determined by certain regulatory mechanisms. The over-expression and repression of various factors responsible for leaf initiation, development, and shape have been previously studied in several mutants. However, in this review, we collectively discuss how these factors modulate leaf development in the context of leaf initiation, polarity establishment, leaf flattening and shape.
Meta-Analysis Reveals Significant Sex Differences in Chronic Lymphocytic Leukemia Progression in the Eµ-TCL1 Transgenic Mouse Model

The Eµ-TCL1 transgenic mouse model represents the most widely and extensively used animal model for chronic lymphocytic leukemia (CLL). In this report, we performed a meta-analysis of leukemia progression in over 300 individual Eµ-TCL1 transgenic mice and discovered a significantly accelerated disease progression in females compared to males. This difference is also reflected in an aggressive CLL mouse model with additional deletion of Tp53 besides the TCL1 transgene. Moreover, after serial adoptive transplantation of murine CLL cells, female recipients also succumbed to CLL earlier than male recipients. This sex-related disparity in the murine models is markedly contradictory to the human CLL condition. Thus, due to our observation we urge both careful consideration in the experimental design and accurate description of the Eµ-TCL1 transgenic cohorts in future studies.
Common principles of early mammalian embryo self-organisation

ABSTRACT Pre-implantation mammalian development unites extreme plasticity with a robust outcome: the formation of a blastocyst, an organised multi-layered structure ready for implantation. The process of blastocyst formation is one of the best-known examples of self-organisation. The first three cell lineages in mammalian development specify and arrange themselves during the morphogenic process based on cell-cell interactions. Despite decades of research, the unifying principles driving early mammalian development are still not fully defined. Here, we discuss the role of physical forces, and molecular and cellular mechanisms, in driving self-organisation and lineage formation that are shared between eutherian mammals. Summary: This Review discusses recent research into the role of physical forces, and molecular and cellular mechanisms, driving self-organisation and lineage formation in eutherian mammals.
llncRNAs as Potential Targets in Small Cell Lung Cancer: Myc Dependent Regulation.

BACKGROUND Small cell lung cancer (SCLC) is a highly aggressive malignancy. MYC family oncogenes are amplified and overexpressed in 20% of SCLCs showing that MYC oncogenes and Myc regulated genes are strong candidates as therapeutic targets for SCLC. c-Myc plays a fundamental role in cancer stem cell properties and malignant transformation. Several targets have been identified by activation/repression of MYC.Deregulated expression levels of lncRNAs have also been observed in many cancers. OBJECTIVE Purpose of the study is to investigate the lncRNA profiles depend on MYC expression levels in SCLC. METHODS First, we constructed lentiviral vectors for Myc overexpression/inhibition. MYC expression is suppressed by lentiviral shRNA vector in MYC amplified H82 and N417 cells and overexpressed by lentiviral inducible overexpression vector in MYC non-amplified H345 cells. LncRNA cDNA is transcribed from total RNA samples and 91 lncRNAs are evaluated by qRT-PCR. RESULTS We obtained that N417, H82 and H345 cells are seem to be addicted to Myc for their growth. Besides, Myc is found to regulate the expressions of genes related to invasion, stem cell properties, apoptosis and cell cycle (p21, Bcl2, cyclinD1, Sox2, Aldh1a1, N-Cadherin) and also lncRNAs. With this respect AK23948, ANRIL, E2F4AS, GAS5, MEG3, H19, L1PA16, SFMBT2, ZEB2NAT, HOTAIR, Sox2OT, PVT1 and BC200 expressions were in parallel with Myc expression whereas Malat1, PTENP1, Neat1, UCA1, SNHG3 and SNHG6 expressions were inversely correlated. CONCLUSION Targeting Myc-regulated genes as a therapeutic strategy can be important for SCLC therapy. This study has importance for identifying Myc-regulated lncRNAs with regards to developing therapeutic strategy in SCLC.
The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells

Microglial cells clear the brain of pathogens and harmful debris, including amyloid-β (Aβ) deposits that are formed during Alzheimer’s disease (AD). We studied the expression of Msr1, Ager and Cd36 receptors involved in Aβ uptake and expression of Cd33 protein, which is considered a risk factor in AD. The effect of silver nanoparticles (AgNP) and cadmium telluride quantum dots (CdTeQD) on the expression of the above receptors and Aβ uptake by microglial cells was investigated. Absorption of Aβ and NP was confirmed by confocal microscopy. AgNP, but not CdTeQD, caused a decrease in Aβ accumulation. By using a specific inhibitor—polyinosinic acid—we demonstrated that Aβ and AgNP compete for scavenger receptors. Real-time PCR showed up-regulation of Cd33 and Cd36 gene expression after treatment with CdTeQD for 24 h. Analysis of the abundance of the receptors on the cell surface revealed that AgNP treatment significantly reduced the presence of Msr1, Cd33, Ager and Cd36 receptors (6 and 24 h), whereas CdTeQD increased the levels of Msr1 and Cd36 (24 h). To summarize, we showed that AgNP uptake competes with Aβ uptake by microglial cells and consequently can impair the removal of the aggregates. In turn, CdTeQD treatment led to the accumulation of proinflammatory Cd36 protein on the cell surface.
Perencanaan Proyek Implementasi Aplikasi Enterprise Resource Planning (ERP) Berbasis Open Source Dengan Odoo : Studi Kasus UMKM Garmen Alifah Collection

UMKM Alifah Collection merupakan UMKM yang bergerak di bidang garmen yang berlokasi di Surabaya. Sehari-harinya UMKM Alifah Collection menerima pesanan pembuatan pakaian mulai dari yang berskala kecil (satuan) hingga yang berskala besar (ribuan). Dengan banyaknya pesanan UMKM Alifah Collection seringkali mengalami kendala seperti hilangnya catatan pesanan dan hilangnya nota pembayaran. Hal tersebut terjadi dikarenakan proses masih dilaksanakan secara manual. Karena itu, pemilik merasa membutuhkan penerapan sebuah sistem terintegrasi dalam hal ini adalah Odoo untuk membantu pelaksanaan proses bisnis pada UMKM Alifah Collection. Sayangnya, implementasi ERP yang banyak dilakukan sekarang jarang sekali melalui perencanaan yang matang sehingga banyak juga yang mengalami kegagalan. Variabel yang paling penting dan bermakna dalam faktor kegagalan implementasi ERP adalah perencanaan yang buruk. Oleh karena itu perlu adanya perencanaan yang baik sebelum melakukan proyek implementasi Odoo. Untuk itu, penelitian ini dilakukan sebagai perencanaan terhadap proyek implementasi Odoo pada UMKM Alifah Collection. Metode yang digunakan dalam tugas akhir ini yaitu metode analisis kesenjangan dimana proses bisnis aktual pada UMKM dibandingkan dengan proses bisnis pada Odoo sehingga terlihat gap. Hasil dari tugas akhir ini menjadi rekomendasi perubahan proses bisnis dan/atau kustomisasi pada proyek implementasi Odoo. ==================================================================================== UMKM Alifah Collection is a SMEs engaged in the field of garment located in Surabaya. Everyday UMKM Alifah Collection receives orders to make clothes ranging from small scale (unit) to a large scale (thousands). As the many orders, UMKM Alifah Collection often facing several problem such as loss of order records and loss of payment note. The problem happens because the process is still done manually. Therefore, the owner think that UMKM Alifah Collection need to implement an integrated system in this case is Odoo to assist the business processes at UMKM Alifah Collection. Unfortunately, many ERP implementations are rarely done through a careful planning as the result many of them failed. The most important and meaningful variable in the failure factor of ERP implementation is a poor planning. It is necessary to have a good planning before undertaking the implementation project of Odoo. Therefore, this research is conducted as a planning for the implementation project of Odoo at UMKM Alifah Collection. The method used in this research is gap analysis method where the actual business process at UMKM Alifah Collection compared with the business process in Odoo and the gap will be visible. The output of this research is a recommendation of business process reengineering and / or customization of Odoo in the implementation project of Odoo at UMKM Alifah Collection.
Glucose Loading Enhances the Value of 18F-FDG PET/CT for the Characterization and Delineation of Cerebral Gliomas

This study aimed to assess how to enhance the value of 18F-Fluorodeoxyglucose (FDG) PET/CTs for glioma grading and better delineation of the tumor boundary by glucose loading. In mouse models of brain tumor using U87MG cells, 18F-FDG-PET images were obtained after fasting and after glucose loading. There was a significant difference in the tumor-to-normal cortex-uptake ratio (TNR) between the fasting and glucose-loading scans. 14C-2-Deoxy-D-glucose (14C-DG) uptake was measured in vitro using U87MG, U373MG and primary neurons cultured with different concentrations of glucose. The tumor-to-neuron ratio of 14C-DG uptake increased with up to 10 mM of glucose. Finally, 10 low-grade and 17 high-grade glioma patients underwent fasting and glucose loading 18F-FDG PET/CT and the TNR was compared between scans. The effect of glucose loading was significant in high-grade but not in low-grade gliomas. The receiver operating characteristic curve analyses with a cut-off TNR of 0.81 showed a higher area under the curve after glucose loading than fasting for differentiating low-grade versus high-grade gliomas. In addition, the glucose loading PET/CT was more useful than the fasting PET/CT for the discrimination of oligodendrogliomas from IDH-wildtype glioblastomas. Glucose loading resulted in a greater reduction in 18F-FDG uptake in the normal cortex than in tumors, which increases the usefulness of 18F-FDG PET/CT for grading.
Evapotranspiração e coeficiente de cultura do algodoeiro irrigado a partir de imagens de sensores orbitais

This research aimed determine cotton evapotranspiration - ETc and crop coefficient - Kc slope using the Surface Energy balance Algorithm for Land - SEBAL with TM-Landsat 5 images. We used eight images distributed throughout the cotton growth season on the Busato Farm located in Bom Jesus da Lapa, Medio Sao Francisco region, Bahia state (13°15'18" S, 43°25'05" W, 436 m). The Climate classification of region by Koppen is BSwh'. The net radiation was calculated from surface temperature, surface emissivity, vegetation index and albedo imagesn and calculated soil and sensible heats fluxes to obtain the latent heat flux and ETc. The NDVI vegetation index presented concurrent development with the crop season, with maximum values (0.80) 70 days after sowing - DAS. Values of Etc and Kc were, respectively, 0 a 5.0 mm day-1 e 0.65 in vegetation development period (7 e 70 DAS); > 6 mm day-1 e 1.18 during flowering and cotton bolls generation; and 2 mm day-1 e 0.66 in the crop cycle ending. Results show that NDVI is a good indicator of the development of cotton and ETc and Kc data are consistent with reports in the literature.
Transcriptional profiling of chickpea genes differentially regulated by salicylic acid, methyl jasmonate and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation.

Using microarray technology and a set of chickpea (Cicer arietinum L.) unigenes and grasspea (Lathyrus sativus L.) expressed sequence tags, chickpea responses to treatments with the defence signalling compounds salicylic acid (SA), methyl jasmonate (MeJA) and aminocyclopropane carboxylic acid (ACC) were studied in three chickpea genotypes with ranging levels of resistance to ascochyta blight [Ascochyta rabiei (Pass.) L. ]. The experimental system minimised environmental effects and was conducted in reference design, where samples from untreated controls acted as references against post-treatment samples. Microarray observations were also validated by quantitative reverse transcription-polymerase chain reaction. The time-course expression patterns of 715 experimental microarray features resulted in differential expression of 425 transcripts. The A. rabiei resistant chickpea genotypes showed a more substantial range of defence-related gene induction by all treatments, indicating that they may possess stronger abilities to resist pathogens. Further, the involvement of SA, MeJA and ACC signalling was identified for the regulation of some important A. rabiei responsive transcripts, as well as cross-talk between these pathways. In the current study we also found evidence to suggest the involvement of A. rabiei-specific signalling mechanisms for the induction of several transcripts that were previously implicated in A. rabiei resistance. This study characterised the regulatory mechanisms of many chickpea transcripts that may be important in defence against various pathogens, as well as other cellular functions. These results provide novel insights to the molecular control of chickpea cellular processes, which may assist the understanding of chickpea defence mechanisms and allow enhanced development of disease resistant cultivars.
Guiding biopsies of pigmented lesions with deep learning

were collected from 50 consecutive (new) patients, with results presented in Table 1. The average age standard deviation at time of clinic attendance was 36.2 17.8 years. Median Fitzpatrick phototype was III with an interquartile range covering skin phototypes II–IV. Referrals from dermatologists made up 38% of visits, with the median (Q1,Q3) patient distance travelled of 9.5 (7,22) kilometres. Two patients travelled ~300 km interstate for their consultation. The majority of cases were referred for vitiligo management (80%). Of the PSQ-18 sub-scale averages, patients scored communication (4.04 0.68), technical quality of services offered (4.00 0.61) highest, with financial aspects (3.39 0.85) and accessibility and convenience (3.49 0.63) the lowest. Satisfaction for the clinic was 67.3 9.0 equating to a ‘good’ overall satisfaction. This study highlights the need for a referral service not only for general practitioners, but also for dermatologists who may require assistance for difficult-to-treat cases and/ or use of equipment not available in standard dermatology practices. We noted a median Fitzpatrick phototype of III (light brown skin). This finding may be due to darker skinned patients presenting for management of vitiligo (80% of presentations) due to noticeable skin contrast, compared to lighter skin phenotypes. The PSQ-18 results indicated good satisfaction for the majority of sub-scales, but lagged on financial aspects. Currently, phototherapy for whole-body/hand-and-foot cabinet is covered under the Medicare Benefits Schedule, whilst the use of the excimer lamp is not. Accessibilty and convenience also scored lower, which may be explained by the extended distances travelled, suggesting a need for additional services within the Sydney catchment. Projections show that Western Sydney will experience >50% growth in its population over the next 20 years (2016– 2036) due to its large cultural diversity. Following this, the service was extended to a secondary site in Westmead to address the issue of accessibility and convenience, though wait times for clinic remain longer than 8 weeks. This study validates patient satisfaction and the need for a dedicated pigmentary disorders clinic in the Australian setting.
Riding an elephant: A qualitative study of nurses' moral journeys in the context of Medical Assistance in Dying (MAiD)

Abstract Aims and objectives To describes nurses' moral experiences with Medical Assistance in Dying in the Canadian context. Background Nurses perform important roles in Medical Assistance in Dying in Canada and do so within a unique context in which Medical Assistance in Dying is provided through healthcare services and where accessibility is an important principle. International literature indicates that participating in Medical Assistance in Dying can be deeply impactful for nurses and requires a high degree of moral sense‐making. Design A qualitative interview study guided by Interpretive Description using the COREQ checklist. Results Fifty‐nine nurses from across Canada participated in the study. The decision to participate in Medical Assistance in Dying was influenced by family and community, professional experience and nurses' proximity to the act of Medical Assistance in Dying. Nurses described a range of deep and sometimes conflicting emotional reactions provoked by Medical Assistance in Dying. Nurses used a number of moral waypoints to make sense of their decision including patient choice, control and certainty; an understanding that it was not about the nurse; a commitment to staying with patients through suffering; consideration of moral consistency; issues related to the afterlife; and the peace and gratitude demonstrated by patients and families. Discussion The depth of nurses' intuitional moral responses and their need to make sense of these responses are consistent with Haidt's theory of moral experience in which individuals use reasoning primarily to explain their moral intuition and in which moral change occurs primarily through compassionate social interaction. Further, work on the moral identity of nursing provides robust explanation of how nurses' moral decisions are contextually and relationally mediated and how they seek to guard patient vulnerability, even at their own emotional cost. Conclusion Medical Assistance in Dying is impactful for nurses, and for some, it requires intensive and ongoing moral sense‐making. Relevance to clinical practice There is a need to provide support for nurses' moral deliberation and emotional well‐being in the context of Medical Assistance in Dying care.
Coded transmissions for space links affected by solar scintillation: Baseband analysis

A thorough analysis of the behavior of error‐correcting codes over space links affected by solar scintillation is presented. The relevant channel parameters are fixed through the development of a model based on real missions' data. Both telecommand and telemetry links are considered in fast and slow fading conditions. Besides classical coherent modulation schemes, the possibility to use noncoherent modulation schemes is considered, to eliminate the problem of phase tracking. For the case of channels with very slow fading, the use of external interleavers or erasure coding is proposed to cope with error bursts.
miR-135b-5p enhances the sensitivity of HER-2 positive breast cancer to trastuzumab via binding to cyclin D2

Trastuzumab has led to a marked improvement in the outcomes of patients with human epidermal growth factor receptor 2 (HER-2)-positive breast cancer. However, the effects of trastuzumab on HER-2-positive breast cancer are limited by the emergence of its cardiotoxicside effects. MicroRNA (miR)-135b-5p has been shown to inhibit tumor metastasis in breast cancer. The present study aimed to explore the effects of miR-135b-5p overexpression on the efficacy of trastuzumab in HER-2-positive breast cancer. Reverse transcription-quantitative PCR was performed to detect the levels of miR-135b-5p. Cell viability was evaluated with a Cell Counting Kit-8 assay. Annexin V/propidium iodide staining was employed to detect the number of apoptotic cells. Flow cytometry assay was performed to investigate the cell cycle. Western blotting was used to detect the expression levels of Bax, cleaved caspase-3, Bcl-2, cyclin D2, p27Kip1 and cyclin E1. Cell migration and invasion were detected by Transwell assay. Luciferase assays were conducted to identify the target gene of miR-135b-5p. In addition, an in vivo tumor xenograft model was established. miR-135b-5p agomir significantly enhanced the anti-proliferative effect of trastuzumab on HER-2-positive breast cancer cells via the induction of apoptosis, whereas the anti-metastatic effect of trastuzumab was enhanced by miR-135b-5p agomir treatment. Subsequently, luciferase assays indicated that cyclin D2 was the direct target of miR-135b-5p, whereas overexpression of the latter arrested cell cycleduring the G0/G1 phase. Moreover, miR-135b-5p agomir notably increased the antitumor effect of trastuzumab in vivo. The data demonstrated that miR-135b-5p sensitized HER-2-positive breast cancer cells to trastuzumab in vitro and in vivo by directly binding to cyclin D2. These results suggested that the combination of miR-135b-5p with trastuzumab may be a therapeutic strategy for patients with HER-2-positive breast cancer.