Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts

Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger accelerated cellular senescence. In this study, a SIPS model was established by exposing human diploid fibroblasts (HDFs) to 20 μM H2O2 for 2 weeks. A proteomic comparison between young, senescent and SIPS cells was done using two dimensional gel electrophoresis (2DGE) to elucidate the changes in protein expression associated with cellular aging. Our analysis showed that 28 protein spots were differentially expressed in senescent cells whereas 10 protein spots were differentially expressed in SIPS as compared to young cells. Three similar protein spots were differentially expressed in both senescent and SIPS cells when compared to the young cells. These results indicate that a difference in protein expression exists between senescent cells and SIPS cells compared to young cells.
Real Options–Based Approach for Valuation of Government Guarantees in Public–Private Partnerships

AbstractA fast and computationally efficient valuation tool assists governments involved in Public–Private Partnership (P3) projects to examine many contractual configurations and design a guarantee that minimizes cost and reasonably mitigates the risk. This paper presents a continuous stochastic process derived from the risk factor forecast, thereby providing a more realistic and flexible model. A new valuation approach is developed by using a finite-difference method based on this continuous stochastic process. In a numerical example with one risk factor, it is shown that this new valuation tool is 100 times faster than the existing simulation-based approach. Its superior speed presents the opportunity to examine different contractual configurations, and as a result, design a more cost effective guarantee contract. Exercise strategies are derived for a multiple-exercise (Australian) guarantees structure. This new approach can be used by a government to reserve budget for the guarantees. Finally, the con.
The Kidney Is a Major Site of a 2-Antiplasmin Production

The serpin a 2 -antiplasmin ( a 2 -AP) is the major circulating inhibitor of plasmin; it plays a determining role in the regulation of intravascular fibrinolysis. In addition to blood plasma, plasmin formation occurs in various organs where it is thought to fulfill a spectrum of functions not restricted to clot lysis. a 2 -AP is synthesized by hepatocytes, but other possible sites of production have not been investigated. To explore the potential extravascular contribution of a 2 -AP in the regulation of proteolysis, we have isolated the murine a 2 -AP cDNA and determined its mRNA distribution in adult tissues. In addition to liver, kidneys are major sites of a 2 -AP mRNA accumulation in the mouse. The transcript is present in epithelial cells lining the convoluted portion of proximal tubules, and its accumulation is under androgen control. Human kidneys also contain high levels of a 2 -AP mRNA. Moderate amounts of a 2 -AP mRNA are detected in other murine tissues such as muscle, intestine, central nervous system, and placenta. Our observations indicate that a 2 -AP can be synthesized in a number of tissues, where it could function as a distal regulator of plasmin-mediated extracellular proteolysis. ( J. Clin. Invest. 1996. 97:2478–2484.)
IGF uptake with competitive binding in articular cartilage

Experiments on the transport of radiolabeled Insulin-like Growth Factors (IGF-I and -II) into bovine articular cartilage show differential uptake depending on the relative proportion of IGF-I and -II. In this study, we present a mathematical model describing both the transport and competition of IGF-I and -II for binding sites represented by two functional groupings of IGF binding proteins (IGFBPs). The first grouping has approximately similar binding affinity to both IGF-I and -II (i.e. IGFBPs 1-5), whereas the second group has significantly higher binding preference for IGF-II compared to IGF-I (i.e. IGFBP-6). Using nonlinear least squares, it is shown that the experimental equilibrium competitive binding results can be described using a reversible Langmuir sorption isotherm involving two dominant IGFBP functional groups. After coupling the sorption model with a poromechanical continuum model, parametric studies are carried out to investigate the effect of model changes including IGF boundary conditions and the ratios of the two IGFBP functional groups. The results show that ignoring competitive binding leads to a significant overestimation of total IGF-I uptake, but an underestimation the rate of "free" (physiologically active) IGF-I within the cartilage. An increase of first group of IGFBPs (i.e. IGFBPs 1-5) as has been reported for osteoarthritis, is observed to hinder the bioavailability of free IGF-I in cartilage, even though the total IGF-I uptake is enhanced. Furthermore, the combination of dynamic compression and competitive binding is seen to enhance the IGF-I uptake within cartilage, but this enhancement is overestimated if competitive binding is neglected.
Registered report: Coding-independent regulation of the tumor suppressor PTEN by competing endogenous mRNAs

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from “Coding-Independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous 'mRNAs' by Tay and colleagues, published in Cell in 2011 (Tay et al., 2011). The experiments to be replicated are those reported in Figures 3C, 3D, 3G, 3H, 5A and 5B, and in Supplemental Figures 3A and B. Tay and colleagues proposed a new regulatory mechanism based on competing endogenous RNAs (ceRNAs), which regulate target genes by competitive binding of shared microRNAs. They test their model by identifying and confirming ceRNAs that target PTEN. In Figure 3A and B, they report that perturbing expression of putative PTEN ceRNAs affects expression of PTEN. This effect is dependent on functional microRNA machinery (Figure 3G and H), and affects the pathway downstream of PTEN itself (Figures 5A and B). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published by eLife. DOI: http://dx.doi. org/10.7554/eLife.12470. 001
Comparison of the predictive performance of adherence measures for virologic failure detection in people living with HIV: a systematic review and pairwise meta-analysis

ABSTRACT A critical feature of an adherence assessment tool is its ability to predict virologic failure in people living with HIV (PLHIV). We, therefore, aimed to compare the predictive performance of commonly used adherence measures. We systematically searched MEDLINE, Embase and LILACS up to February 2018, to identify relevant observational studies comparing the effects of any two of the following adherence measurements on virologic outcomes: electronic monitoring, pill count, pharmacy refill, self-report and physician assessment. We analyzed data by pairwise meta-analyzes with a random-effects model. The proportion of virologic failures among non-adherent participants in each adherence measure was used to calculate the odds ratio (OR), with 95% Confidence Intervals (95%CI). Heterogeneity was assessed, with potential causes identified by sensitivity and subgroup analysis. We included 38 studies with individual patient data for 18,010 patients. All possible comparisons between pairs of the five adherence measures were considered and a total of nine comparison groups could be established. Meta-analysis suggested that self-report was a better predictor of virologic failure than pill count when the recall period was within one week (OR: 2.35, 95%CI: 1.07–5.18, p = 0.03). Physician assessment had higher odds of predicting virologic failure than did either self-report (OR: 2.63, 95%CI: 1.37–5.26, p < 0.01) or pharmacy refill (OR: 3.57, 95%CI: 1.69–7.14, p < 0.001). There was no difference in the predictive performance between any of the other measures that we were able to compare (p > 0.05). The combination of multiple measures did not increase the predictive value when compared to any of the measures alone. Low-cost and simple adherence measures such as self-report predict virologic failure better than or equally well as objective measures. Our results suggest that there is no need to use expensive or time-consuming adherence measures when the objective is to identify PLHIV at risk of treatment failure.
A Novel Application of a Cryosectioning Technique to Aid Scat Hair Microanalysis

Scat hair presents a diverse profile of hairs for morphological assessment that may find versatile applications in wildlife forensic investigations. Successful morphological assessment of scat hair microstructure, however, depends on a robust sectioning methodology. We assessed the feasibility and efficacy of a cryosectioning technique compared to that of a gold standard hand‐sectioning technique. Scat hairs were embedded in paraffin wax and hand‐sectioned, while cryopreserved scat hairs were sectioned with a cryostat. The results showed that cryosectioning preserved the pristine morphology of the scat hair and provided cross sections more amenable to high‐resolution imaging of hair internal microstructure than hand‐sectioning. The cryosectioning technique may find novel applications as a more reliable and robust technique to aid (i) scat hair internal microstructure analysis for cross‐referencing with species identification keys in wildlife forensic studies and (ii) downstream toxicological analysis in wildlife forensic studies as hair biochemistry is not altered during cryopreservation.
Sickle Cell and α+-Thalassemia Traits Influence the Association between Ferritin and Hepcidin in Rural Kenyan Children Aged 14-26 Months.

Background The relation between subclinical hemoglobinopathies and concentrations of the iron-regulatory hormone hepcidin is not well characterized. Objective We investigated the relation of hepcidin concentration with hemoglobinopathies among young children in Kenya. Methods We quantified serum hepcidin and ferritin in 435 Kenyan children aged 14-20 mo in a subsample of the Water, Sanitation, and Handwashing (WASH) Benefits Trial. Blood samples were genotyped for α+-thalassemia and for sickle cell disorder. Hepcidin was compared across sickle cell and α+-thalassemia genotypes separately by using generalized linear models, and children who were normozygous for both conditions were also compared with those who had either of these conditions. In the association between hepcidin and ferritin, we assessed effect modification by genotype. Results In this population, we found that 16.2% had sickle cell trait and 0.2% had sickle cell disorder, whereas 40.0% were heterozygous for α+-thalassemia and 8.2% were homozygous. Hepcidin concentration did not differ by genotype, but effect modification was found by genotype in the association between hepcidin and ferritin (P < 0.1). Among normozygous sickle cell children (HbAA), there was an association between hepcidin and ferritin (β = 0.92; 95% CI: 0.72, 1.10). However, among those with sickle cell trait (HbAS), the association was no longer significant (β = 0.31; 95% CI: -0.04, 0.66). Similarly, among children who were normozygous (αα/αα) or heterozygous (-α/αα) for α+-thalassemia, hepcidin and ferritin were significantly associated [β = 0.94 (95% CI: 0.68, 1.20) and β = 0.77 (95% CI: 0.51, 1.03), respectively]; however, in children who were homozygous for α+-thalassemia (-α/-α), there was no longer a significant association (β = 0.45; 95% CI: -0.10, 1.00). Conclusion Hepcidin was not associated with hemoglobin genotype, but there may be a difference in the way hepcidin responds to iron status among those with either sickle cell trait or homozygous α+-thalassemia in young Kenyan children. This trial was registered at clinicaltrials.gov as NCT01704105.
Reconstruction of Lightweight 3D Indoor Representations using Color-Depth Cameras

The current bandwidth and traffic limits on mobile data connections make it infeasible, for domestic surveillance robots, to regularly send the data acquired on the surveilled environment to mobile devices, ultimately defeating their purpose. In this work we propose developing a methodology to build global, lightweight 3D indoor representations using the range information returned by a low-cost RGB-D camera, so that they are compact enough to be easily transferable to mobile devices. We detail methods to rapidly segment range images into planar patches, use them to estimate camera motion and merge them into a global 3D model.
Degradation of polycyclic aromatic hydrocarbons (PAHs) employing biosurfactant producing Pseudomonas aeruginosa KS3

An efficient biosurfactant-producing/hydrocarbon-degrading native bacterial strain Pseudomonas aeruginosa KS3 (identified by partial 16S rDNA gene sequencing) was isolated from crude oil contaminated soil collected from the oil field of Lakowa in Sivasagar district of Assam, India. Experiments were conducted in the laboratory to determine the efficiency of strain KS3 to degrade polycyclic aromatic hydrocarbons (PAHs). Further, biosurfactant production was measured based on surface tension (ST) reduction of culture media. The results of the study show that this native biosurfactant producing bacterial strain has great potentiality in the degradation of total petroleum hydrocarbon (TPH) and PAHs, which have great prospects in remediation of hydrocarbon from contaminated sites. It was found that the strain KS3 could degrade 79.16% of TPH in 4 wk of incubation time. The strain also demonstrated efficient degradation of PAHs. Among the 16 major PAHs present in the crude oil sample, strain KS3 could completely degrade 8 of them. Biochemical and FTIR analyses confirmed that the produced biosurfactant was glycolipid in nature.
Association between LMP2/LMP7 genetic variability and cancer susceptibility, especially among Asians: evidence from a meta-analysis

Low molecular mass protein (LMP) gene performs a critical role in the foreign antigen processing machine via the major histocompatibility complex-I (MHC-I) complex CD8+ cytotoxic T lymphocytes (CTL) pathway. Recent studies have reported the association of LMP2-60 G>A (rs17587) and LMP7-145 C>A (rs2071543) polymorphisms with various types of cancers, but the outcomes remained inconsistent. To obtain a reliable conclusion, we summarized available data and conducted a meta-analysis involving a total of 19 published studies. Evidences were obtained from the PubMed, Google Scholar, Web of Science and Chinese National Knowledge Infrastructure (CNKI) databases. The results demonstrated that the rs17587 and rs2071543 polymorphisms were associated with an increased cancer risk in the recessive and homozygote models. Stratified analyses by ethnicity indicated a significant association only in Asian population. Furthermore, rs17587 showed a greater susceptibility to gynecological cancers, while rs2071543 increased the risk of gastrointestinal and gynecological cancers. Our results indicate that the LMP2 rs17587 and LMP7 rs2071543 polymorphisms may act as risk factors for cancer, especially for Asian populations. Additional larger-scale multicenter studies should be performed to validate our results.
T lymphocyte locomotion in a three-dimensional collagen matrix. Expression and function of cell adhesion molecules.

T cell locomotion within the extracellular matrix may be mediated by cell adhesion molecules. We investigated the expression and function of beta 1- and beta 2-integrins and CD44 on human peripheral CD4+ and CD8+ lymphocytes locomoting in a 3-D type I collagen matrix. Paths of randomly selected T cells were digitized from time-lapse videorecordings and were quantitatively analyzed. After the blocking of CD49b with mAb Gi9, the locomotion of a defined locomotor subset (50% of spontaneously locomoting cells) was inhibited. Anti-CD49d mAb HP2/1 and an activating anti-CD44 mAb (J173), respectively, induced transient recruitment (< 1 h) of previously nonmotile cells (10 to 35%). In contrast to the J173-induced short-term locomotion, hyaluronan incorporated within the matrix promoted locomotion for > 2 h. No significant effects were present for anti-CD49f (GoH3) and -CD11a (25.3) mAbs. After the addition of IL-8 to the matrix, rapid induction of locomotion in 20 to 30% of the cells (control) was evident, which was virtually abolished by anti-alpha 2- and alpha 6-integrin, and -CD11a mAbs. Thus, the locomotion of nonactivated and IL-8-activated T cells may involve different sets of integrins. Using flow cytometry, the development of a CD49b+CD29highCD44lowL-selectinlow T cell phenotype independent of activation markers including CD25, CD27, CD28, VLA-4, and CD45RA- to CD45RO-transition was observed after 4 days in the matrix. The initial development of spontaneous locomotion in the collagen matrix, however, was not accompanied by alterations in CAM surface staining and, therefore, may involve functional CAM activation rather than involving an increase in surface expression.
Empirically testing the relationship between income distribution, perceived value money and pay satisfaction

Compensation management literature highlights that income has three major features: salary, bonus and allowance. If the level and/or amount of income are distributed to employees based on proper rules this may increase pay satisfaction. More importantly, a thorough investigation in this area reveals that the effect of income distribution on pay satisfaction is not consistent if perceived value of money is present in organizations. The nature of this relationship is less emphasized in pay distribution literature. Therefore, this study was conducted to measure the effect of the perceived value of money and income distribution on pay satisfaction using 136 usable questionnaires gathered from employees who have worked in one city based local authority in Sabah, Malaysia (MSLAUTHORITY). Outcomes of hierarchical regression analysis showed that the interaction between perceived value of money and income distribution significantly correlated with pay satisfaction. This result confirms that perceived value of money does act as a moderating variable in the income distribution model of the organizational sample. In addition, discussion and implications of this study are elaborated.
Assessing the importance of High Nature Value farmlands for the conservation of Lesser Kestrels Falco naumanni

Abstract Agricultural areas, such as cereal cultivations, that support species of European and/or national conservation concern are considered as ‘High Nature Value’ farmlands (HNVf) and are very important for the preservation of biodiversity in Europe. The lesser kestrel Falco naumanni is a migratory falcon breeding largely in the HNVf of the Mediterranean basin. The main cause of its decline in Europe has been habitat loss and degradation as a result of agricultural intensification driven largely by the EU Common Agricultural Policies (CAP). In Greece, its population dropped by about 50% since the 1970s and its preferred habitats have shrunk. The aim of this study was to assess habitat preferences of breeding Lesser Kestrels in agro-ecosystems of Greece and relate these habitats to HNVf for conservation purposes. The study area is located in the plain of Thessaly, Central Greece, holding the main lesser kestrel breeding populations in the country, where dry cereal crops have been significantly depleted over the past decades. Species distribution models were developed with generalised additive models for the analyses. Predicted probability of lesser kestrel occurrence was found to be positively associated with farmed landscapes of dry cereal cultivations. Other important predictors were cultivated irrigated farmland and landscape heterogeneity. Main results of the statistical models agree with the findings of other habitatbased studies that highlight the importance of low-input farming systems, that is, HNVf, for safeguarding vital Lesser Kestrels habitats in their breeding grounds in the Mediterranean. A key conservation priority for conserving species dependant on HNVf is the maintenance of those low-input farming systems and the implementation of a greener CAP that would promote environmental-friendly farming practices to preserve and enhance biodiversity in the agro-ecosystems of Europe.
TWO-COMPONENT CYCLOTRON RESONANCE IN QUANTUM HALL SYSTEMS

Effects of electron-electron interactions on spin-split and mass-split cyclotron resonance ~CR! in a quantum Hall regime are studied by means of numerical diagonalization in finite-size systems. In the case of spin-split CR, the spectra at low temperatures show a simple mode-repulsion behavior at low filling factors, while they show more complicated behaviors at high filling factors including motional narrowing when the electron density for up-spin and that for down-spin are comparable. The spectra at high temperatures can be understood in terms of increase in the effective filling factor. The results qualitatively explain experiments in GaAs/AlxGa12xAs heterostructures. In the case of mass-split CR, the spectra show only a mode-repulsion behavior independent of temperatures and filling factors. @S0163-1829~98! 06627-2#
3D Radiation Pattern Reconfigurable Phased Array for Transmission Angle Sensing in 5G Mobile Communication

This paper proposes a 3D radiation pattern reconfigurable antenna (RPRA) and a reconfigurable phased array (RPA) for 5G mobile communication. The antenna and array are working at 28 GHz, which is selected as a 5G communication band in many countries. The proposed phased array will be applied as sensors to find out the optimal transmitting–receiving angle in a randomly changed cellular wireless scenarios. The RPRA and RPA are fed by Substrate Integrated Waveguide (SIW) and have three switchable radiation modes: Broadside 1, Broadside 2 and Endfire. The three modes correspond to three different radiation patterns and each of them covers a different area in the Azimuth plane. An eight-element phased array constructed by the proposed RPRA, which is able to switch beam in Azimuth plane and scan in the Elevation plane, is also presented in this paper. The proposed RPA is able to provide much higher spatial coverage than the conventional phased arrays and without additional feeding and phase shifting networks. The beam switching is realized by the PIN diodes. The proposed antenna and array have planer structures and require small clearance on the ground plane which makes them compatible with mobile phones. The simulations show good performance for both RPRA and RPA.
Placental Transfer of Rilpivirine in an Ex Vivo Human Cotyledon Perfusion Model

ABSTRACT Placental transfers of the HIV nonnucleoside reverse transcriptase inhibitor rilpivirine were investigated in 8 term human cotyledons perfused with rilpivirine (400 ng/ml) in the maternal-to-fetal direction. The mean fetal transfer rate (FTR) (fetal/maternal concentration at steady state from 15 to 90 min) was 26% ± 8% (mean ± standard deviation), and the clearance index (rilpivirine FTR/antipyrine FTR) was 61% ± 20%. This shows that rilpivirine crosses the placenta at a relatively high rate, suggesting that the fetus is exposed to the compound during treatment of the mother.