Miracle-dz/UniFAR-FTUs huggingface dataset:
Visual outcome and major surgical complications of extracapsular cataract extraction versus manual small-incision cataract surgery performed by resident doctors at tertiary care institute as part of learning curve

Background: Cataract surgery has its own learning curve to the resident doctors. Our resident doctors are trained in extracapsular cataract extraction (ECCE) and manual small-incision cataract surgery (MSICS). In ECCE and initial cases of MSICS, they are trained to do can-opener capsulotomy (COC) and then they are trained in continuous curvilinear capsulorrhexis (CCC). Aim: We designed a study to compare the visual outcome and major surgical complications of resident doctor performed ECCE versus MSICS at our institute. Setting and Design: This was a hospital-based study in ophthalmology department (retrospective study). Materials and Methods: A total of 239 patients with visually significant cataract presenting to our outpatient department were enrolled in the study. Data of consecutive cataract surgeries done by 10 junior residents (JRs) from March to August 2017 were analyzed. First-year residents were trained for ECCE (JR1 group) for 6 months. During the same time period, 2nd-year residents were trained for MSICS (with COC) for first 3 months (JR2 group). Then, as a part of learning curve, 2nd-year residents were then trained for MSICS (with CCC) for next 3 months (JR3 group). Data were analyzed using the Chi-square test, percentages. Results: Out of 239 cataract surgeries performed by residents over a 6-month period, ECCE were 52 (21.8%) and MSICS were 187 (78.2%) cases. Both the surgeries in MSICS (2nd-year residents) and ECCE (1st-year residents) had good visual outcome (94.6% vs. 84.6%). The overall major surgical complication rate for MSICS was 7.0% while that for ECCE was 11.5%. Conclusion: We conclude that both surgeries can be taught to beginner surgeons with good visual outcome. Both surgeries have safe learning curve with good visual results when done under supervision as evidenced by a low rate of major surgical complications.
Trauma, psychiatric disorders, and treatment history among pregnant African American women.

OBJECTIVE Pregnant African American women living in low-income urban communities have high rates of trauma exposure and elevated risk for the development of trauma-related disorders, including posttraumatic stress disorder (PTSD) and depression. Yet, engagement in behavioral health services is lower for African American women than Caucasian women. Limited attention has been given to identifying trauma exposure and PTSD, especially within at-risk communities. The present study examined rates of trauma exposure, PTSD, depression, and behavioral health treatment engagement in an obstetrics/gynecology (OB/GYN) clinic within an urban hospital. METHOD The study included 633 pregnant African American women screened within the OB/GYN clinic waiting room; 55 of the women also participated in a subsequent detailed clinical assessment based on eligibility for a separate study of intergenerational risk for trauma and PTSD in African American mother-child dyads. RESULTS Overall, 98% reported trauma exposure, approximately one third met criteria for probable current PTSD, and one third endorsed moderate-or-severe depression based on self-report measures. Similar levels were found based on clinical assessments in the subsample. While 18% endorsed depression treatment, only 6% received treatment for PTSD. In a subsample of women with whom chart reviews were conducted (n = 358), 15% endorsed a past psychiatric diagnosis but none shared their PTSD diagnosis with their OB/GYN provider. CONCLUSION Results of the current study highlight elevated levels of trauma exposure, PTSD, and depression in low-income, African American pregnant women served by this urban clinic, and demonstrate the need for better identification of trauma-related disorders and appropriate linkage to culturally responsive care especially for PTSD. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
The ER protein TLC domain 3B2 and its enzymatic product lactosylceramide enhance chondrocyte maturation

ABSTRACT Purpose/Aim of study: We previously cloned Tlcd3b2 (Tram-Lag1-CLN8 domain 3B2, formerly Fam57b2) from bone fracture repair callus tissue of Cyp24a1 knockout mice and showed that it synthesizes lactosylceramide (LacCer) under allosteric control of the vitamin D metabolite, 24,25-dihydroxyvitamin D3 [24,25(OH)2D3]. Tlcd3b2 was mainly detected in chondrocytes and the 24,25(OH)2D3-TLCD3B2-LacCer signaling cascade was shown to be important for optimal bone fracture repair, suggesting a role for TLCD3B2 in chondrocyte differentiation or maturation. We report the subcellular localization of TLCD3B2 and its effect on chondrocyte differentiation. Materials and Methods: Immunofluorescence detection of epitope-tagged mutants was used to assess localization. ATDC5 chondrogenic cells were transfected with Tlcd3b2 expression vectors to examine effects on chondrocyte differentiation. Results and Conclusions: TLCD3B2 localized to the endoplasmic reticulum, with both the N- and C-termini facing the cytosolic compartment. Chondrogenic ATDC5 cells stably overexpressing Tlcd3b2 showed elevated type 2 (Col2a1) and type 10 (Col10a1) collagen gene expression and increased proteoglycan synthesis, and the effect on Col2a1 was enhanced by treatment with 24,25(OH)2D3. LacCer treatment of ATDC5 cells potentiated Col10a1 expression. Our results show that TLCD3B2 is an ER protein and implicate its expression and enzymatic product in chondrocyte maturation.
The magnitude of hypertension and its risk factors in southern Ethiopia: A community based study

Background Prevention and control of hypertension has not been given due attention though previous studies indicated that hypertension is growing public health problem. Objective This study aimed to determine the prevalence of hypertension and associated factors in Bona district, southern Ethiopia. Methods A community based cross-sectional study was conducted on 1952 participants aged ≥25 years in Bona District, southern Ethiopia. Data were collected from consented participants recruited using multistage sampling technique. Data were entered, checked for quality and analyzed by SPSS for Windows version 20.0. Since the outcome variables were ordered categorical, we used multinomial logistic regression model to identify associated factors. Among the independent variables included in the model no multicolinearity was observed. The level of significance was set at P value ≤ 0.05. Results The observed prevalence of hypertension (21.8%) was remarkable in rural setting. Out of hypertensive participants, 195 (45.9%) were newly diagnosed. About one third of the participants (31.4%) had central obesity measured by waist-to-height ratio ≥0.50. Being male, age advancement, high BMI (≥25.0 kg/m2) and central obesity (waist-to-height ratio ≥0.50) were positively associated with both systolic and diastolic hypertension. Systolic hypertension was negatively associated with high family income. The likely hood of developing diastolic hypertension increased in participants with family history of hypertension. Conclusion The overall prevalence of hypertension, 21.8%, is alarmingly high that it can be said that hypertension is becoming a silent epidemic in Ethiopia. Nationwide survey is needed to get the clear magnitude of hypertension so that early detection and management strategies can be enforced.
Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis

Sepsis is a potentially life-threatening condition, and it is frequently complicated by myocardial damage. Data on myocardial damage in rabbit caecal ligation and puncture (CLP) models are limited, although numerous animal models have been used to study sepsis-associated myocardial damage. This study aimed to investigate the effect of CLP on cardiac muscle by measuring serum cardiac troponin I (cTnI) concentrations and by detecting both histopathological changes and cTnI immunoreactivity in cardiomyocytes in rabbits. After CLP was performed in rabbits, blood samples were taken from the jugular vein at 0, 4, 8, and 12 h for haematological and biochemical analyses. At the end of the experiment, all of the rabbits were euthanised to examine the histopathological changes and the cTnI immunoreactivity in cardiac muscle tissue. No changes in serum cTnI concentration were observed in the experimental group (EG) or control group (CG) at 0 and 4 h. In EG, the mean serum cTnI concentrations were 0.230 ± 0.209 and 1.177 ± 0.971 ng/ml at 8 and 12 h, respectively. In CG, the mean serum cTnI concentrations were 0.032 ± 0.014 and 0.031 ± 0.021 ng/ml at 8 and 12 h, respectively. Moreover, cytoplasmic cTnI immunoreactivity decreased in EG compared with that in CG (P<0.01). The results demonstrated that CLP induced a systemic inflammatory response and caused myocardial damage in rabbits.
Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training

Simple Summary The aim of this study was to investigate whether endurance training changes pro- and anti-inflammatory cytokine profile within a 20-week training season in young inexperienced endurance Arabian horses. It has been suggested that regular endurance training can induce an advanced anti-inflammatory response, but its nature is not completely understood. However, to promote more effective use of endurance exercise training in health promotion and disease prevention, a complete understanding of the nature of its immune regulatory effect is required. The results suggest that endurance training can induce advanced overall anti-inflammatory response as an adaptation to increasing workload. Abstract Development of an anti-inflammatory state during physical training has been postulated in both human and equine athletes, but it is not completely understood. The aim of this study was to investigate whether endurance training changes pro- and anti-inflammatory cytokine profiles within a 20-week training season in young inexperienced endurance horses. Nine Arabian horses were examined in this prospective 20-week follow-up study. Blood samples were analysed 5 times monthly, at rest and after training sessions. Routine haematological examinations were performed. Cytokine patterns including IL-1β, IL-6, TNF-α, IL-10 mRNA expression using Real Time-PCR, and serum concentrations of IL-1β, IL-2, IL-4, IL-6, IL-17, INFγ, TNF-α, and IL-10 by ELISA test were determined. During endurance training, the most significant decrease in post-exercise cytokine type 1 levels (TNFα and IL-β) occurred within 20 weeks, beginning from the 3rd month of training. IL-6 serum level decreased after the 4th month. The results suggest that endurance training can induce advanced overall anti-inflammatory response as an adaptation to increasing workload.
An Analysis of Feature Selection Methods for Multiclass Text Classification

: - To classify objects into different classes, feature plays a vital role. So identification of best features is a backbone of classification process. In text classification, features are simple words, having very large dimension so finding the most appropriate feature set is a big challenge. This paper includes analysis of some feature selection methods for multi class text classification and checks their results on different classifier for an email classification. We run our experiments on 20NewGroups and PU corpora datasets. Experiments are done on some well-known feature selection method like Term Selection, Document Frequency, Mutual Information, Odds Ratio, Chi square and etc. This paper concludes that Mutual Information and Chi square are most appropriate for text classification.
Structure model and toxicity of the product of bio-dissolution of chrysotile asbestos in the lungs.

Asbestos is a commercial term indicating six natural silicates with asbestiform crystal habit. Of these, five are double-chain silicates (amphibole) and one (serpentine asbestos or chrysotile) is a layer silicate. Although all species are classified as human carcinogens, their degree of toxicity is still matter of debate. Amphibole asbestos species are biopersistent in the human lungs and exert their chronic toxic action for decades, whereas chrysotile is not biopersistent and it transforms into an amorphous silica structure prone to chemical/physical clearance when exposed to an acidic environment created by the alveolar macrophages. There is evidence in the literature of the toxicity of chrysotile, but its limited biopersistence is thought to explain the difference in toxicity with respect to amphibole asbestos. To date, no comprehensive model describing the toxic action of chrysotile in the lungs is available, as the structure and toxic action of the metastable product formed by the bio-dissolution of chrysotile are unknown. This work is aimed at fulfilling this gap and to explain the toxic action in terms of structural, chemical and physical properties. We show that chrysotile's fibrous metastable structure induces cellular damage, mainly through physical interactions. Based on our previous work and novel findings we propose the following toxicity model: inhaled chrysotile fibers exert their toxicity in the alveolar space by physical and bio-chemical action. The fibers are soon leached by the intracellular acid environment into a metastable product with residual toxicity, and the dissolution process liberates toxic metals in the intracellular and extracellular environment.
Berberine and palmatine inhibit the growth of human rhabdomyosarcoma cells

ABSTRACT A natural isoquinoline alkaloid, berberine, has been known to exhibit anti-tumor activity in various cancer cells via inducing cell cycle arrest. However, it has not been investigated whether berberine and its analogs inhibit the growth of rhabdomyosarcoma (RMS), which is the most frequent soft tissue tumor in children. The present study examined the anti-tumor effects of berberine and palmatine on expansions of three human embryonal RMS cell lines; ERMS1, KYM1, and RD. Intracellular incorporation of berberine was relatively higher than that of palmatine in every RMS cell line. Berberine significantly inhibited the cell cycle of all RMS cells at G1 phase. On the other hand, palmatine only suppressed the growth of RD cells. Both of berberine and palmatine strongly inhibited the growth of tumorsphere of RD cells in three-dimensional culture. These results indicate that berberine derivatives have the potential of anti-tumor drugs for RMS therapy. Abbreviations: ARMS: alveolar rhabdomyosarcoma; ERMS: embryonal rhabdomyosarcoma; RMS: rhabdomyosarcoma Graphical abstract An isoquinoline alkaloid, berberine, inhibits growth of rhabdomyosarcoma cells in three-dimensional culture by modulating cell cycle gene expression.
Patient‐Specific Analysis of Neural Activation During Spinal Cord Stimulation for Pain

Despite the widespread use of spinal cord stimulation (SCS) for chronic pain management, its neuromodulatory effects remain poorly understood. Computational models provide a valuable tool to study SCS and its effects on axonal pathways within the spinal cord. However, these models must include sufficient detail to correlate model predictions with clinical effects, including patient‐specific data. Therefore, the goal of this study was to investigate axonal activation at clinically relevant SCS parameters using a computer model that incorporated patient‐specific anatomy and electrode locations.
The rollout of Community ART Refill Groups in Zimbabwe: a qualitative evaluation

Community ART Refill Groups (CARGs) are an antiretroviral therapy (ART) delivery model where clients voluntarily form into groups, and a group member visits the clinic to collect ART for all group members. In late 2016, Zimbabwe began a nationwide rollout of the CARG model. We conducted a qualitative evaluation to assess the perceived effects of this new national service delivery model.
THE EFFECTS OF PAY DISPERSION AND DEMOGRAPHIC SIMILARITY ON EMPLOYEE TURNOVER

Scholars have long recognized that individuals compare their rewards to those received by similar others and that satisfaction is determined, in part, by perceptions of (un)fairness that arise from these comparisons. Extensive research has also found that individuals frequently select others as pay referents who share the same race and the same gender, suggesting that demographic similarity strongly affects equity perceptions. Leveraging this insight, we examine whether the proportion of others in one’s work unit who are of the same race or gender moderates the relationship between horizontal pay dispersion and voluntary turnover. Based on a unique, single-firm sample of workers employed by a Fortune 50 organization, the results show that the relationship between pay dispersion and turnover is moderated by the degree of racial and gender similarity that workers share with work-unit peers, such that the positive relationship between pay dispersion and voluntary turnover becomes stronger when work-unit demographic similarity is higher. Supplemental analyses suggest that this pattern holds for males, females, and whites but not for non-whites. These analyses also provide additional empirical evidence that our findings most likely result from the propensity of males, females, and whites to compare themselves to same-category others.
Pooled Genomic Screens Identify Anti-apoptotic Genes as Targetable Mediators of Chemotherapy Resistance in Ovarian Cancer

High-grade serous ovarian cancer (HGSOC) is often sensitive to initial treatment with platinum and taxane combination chemotherapy, but most patients relapse with chemotherapy-resistant disease. To systematically identify genes modulating chemotherapy response, we performed pooled functional genomic screens in HGSOC cell lines treated with cisplatin, paclitaxel, or cisplatin plus paclitaxel. Genes in the intrinsic pathway of apoptosis were among the top candidate resistance genes in both gain-of-function and loss-of-function screens. In an open reading frame overexpression screen, followed by a mini-pool secondary screen, anti-apoptotic genes including BCL2L1 (BCL-XL) and BCL2L2 (BCL-W) were associated with chemotherapy resistance. In a CRISPR-Cas9 knockout screen, loss of BCL2L1 decreased cell survival whereas loss of proapoptotic genes promoted resistance. To dissect the role of individual anti-apoptotic proteins in HGSOC chemotherapy response, we evaluated overexpression or inhibition of BCL-2, BCL-XL, BCL-W, and MCL1 in HGSOC cell lines. Overexpression of anti-apoptotic proteins decreased apoptosis and modestly increased cell viability upon cisplatin or paclitaxel treatment. Conversely, specific inhibitors of BCL-XL, MCL1, or BCL-XL/BCL-2, but not BCL-2 alone, enhanced cell death when combined with cisplatin or paclitaxel. Anti-apoptotic protein inhibitors also sensitized HGSOC cells to the poly (ADP-ribose) polymerase inhibitor olaparib. These unbiased screens highlight anti-apoptotic proteins as mediators of chemotherapy resistance in HGSOC, and support inhibition of BCL-XL and MCL1, alone or combined with chemotherapy or targeted agents, in treatment of primary and recurrent HGSOC. Implications: Anti-apoptotic proteins modulate drug resistance in ovarian cancer, and inhibitors of BCL-XL or MCL1 promote cell death in combination with chemotherapy.
Exploring the Professional Responsibility Concern Process

Supplemental Digital Content is Available in the Text. Background: Registered nurses are pivotal in the analysis, recognition, and reporting of patient safety issues before harm occurs to patients. Little has been written about the relationship between the professional responsibility of reporting safety concerns and the processes that exist. Problem: More needs to be known about how nurses can best report factors in work environments that impact patient safety. Learning more about processes that exist amidst literature that illuminates the issues related to reporting and patient safety culture is needed. Also, best practice or key exemplars depicting how professional responsibility has been implemented are needed. Limited has been written exploring professional responsibility concern processes in Canada and internationally. Approach: We completed a case study exploration comprising a public facing Web site scan of information about professional responsibility-like processes across Canada, as well as an extensive literature search exploring factors that are linked with nurse reporting of patient safety concerns. Conclusion: Themes from related literature identify patient safety culture, leadership qualities, communication, positive nurse factors, speaking up, and whistle-blowing as important aspects that facilitate, or are related to, the ability for nurses to express professional responsibility concerns. Alberta has a well-developed system of reporting such concerns; however, the lack of research and literature on these topics requires additional focus in nursing internationally.
Incidence of Surgical Site Infection After Spine Surgery: A Systematic Review and Meta-Analysis.

STUDY DESIGN A systematic review and meta-analysis OBJECTIVE. : The objective of this study was to investigate the incidence of surgical site infection (SSI) in patients following spine surgery and the rate of microorganisms in these cases. SUMMARY OF BACKGROUND DATA Many studies have investigated the incidence and risk factors of SSI following spinal surgery, no meta-analysis studies have been conducted regarding the comprehensive epidemiological incidence of SSI after spine surgery. METHODS We searched the PubMed, Embase, and Cochrane Library databases for relevant studies that reported the incidence of SSI after spine surgery, and manually screened reference lists for additional studies. Relevant incidence estimates were calculated. Subgroup analysis, sensitivity analysis and publication bias assessment were also performed. RESULTS Our meta-analysis included 27 studies, with 603 SSI cases in 22475 patients. The pooled SSI incidence was 3.1%. Subgroup analysis revealed that the incidence of superficial SSI was 1.4% and the incidence of deep SSI was 1.7%. Highest incidence (13.0%) was found in patients with neuromuscular scoliosis among the different indications. The incidences of SSI in cervical, thoracic and lumbar spine were 3.4%, 3.7% and 2.7%, respectively. Compared with posterior approach surgery (5.0%), anterior approach showed a lower incidence (2.3%) of SSI. Instrumented surgery had a higher incidence of SSI than non-instrumented surgery (4.4% versus 1.4%). Patients with minimally invasive surgery (1.5%) had a lower SSI incidence than open surgery (3.8%). Lower incidence of SSI was found when vancomycin powder was applied locally during the surgery (1.9%) compared with those not used (4.8%). In addition, the rates of Staphylococcus aureus, Staphylococcus epidermidis and methicillin-resistant Staphylococci in microbiological culture results were 37.9%, 22.7% and 23.1%, respectively. CONCLUSIONS The pooled incidence of SSI following spine surgery was 3.1%. These figures may be useful in the estimation of the probability of SSI following spine surgery. LEVEL OF EVIDENCE 3.