Miracle-dz/UniFAR-FTUs huggingface dataset:
Glow Discharge Plasma Treatment on Zirconia Surface to Enhance Osteoblastic-Like Cell Differentiation and Antimicrobial Effects

Peri-implantitis is the pathological condition of connective tissue inflammation and the progressive loss of supporting bone around dental implants. One of the primary causes of peri mucositis evolving into peri-implantitis is bacterial infection, including infection from Porphyromonas gingivalis. Enhancing the surface smoothness of implants helps to prevent P. gingivalis adhesion to the implant’s surface. Interaction analyses between bacteria and the surface roughness of zirconia (Zr) discs subjected to a glow discharge plasma (GDP) treatment compared with non-plasma-treated autoclaved control Zr discs were done. Examinations of the material prosperities revealed that the GDP-treated Zr group had a smoother surface for a better wettability. The GDP-treated Zr discs improved the proliferation of the osteoblast-like cells MG-63, and the osteoblastic differentiation was assessed through alkaline phosphatase detection and marker gene bone sialoprotein (Bsp) and osteocalcin (OC) induction. Scanning electron microscopy demonstrated a relatively low P. gingivalis adhesion on GDP-treated Zr disks, as well as lower colonization of P. gingivalis compared with the control. Our findings confirmed that the GDP treatment of Zr discs resulted in a significant reduction of P. gingivalis adhesion and growth, demonstrating a positive correlation between surface roughness and bacteria adhesion. Therefore, the GDP treatment of Zr dental implants can provide a method for reducing the risk of peri-implantitis.
Intrinsic Effect of Pyridine-N-Position on Structural Properties of Cu-Based Low-Dimensional Coordination Frameworks

Metal-organic assemblies have received significant attention for catalytic and other applications, including gas and energy storage, due to their porosity and thermal/chemical stability. Here, we report the synthesis and physicochemical characterization of three metallosupramolecular assemblies consisting of isomeric ambidentate pyridyl-β-diketonate ligands L1–L3 and Cu(II) metal ions. It has been demonstrated that the topology and dimensionality of generated supramolecular aggregates depend on the location of the pyridine nitrogen donor atom in L1–L3. This is seen in characterization of two distinct 2D polymeric assemblies, i.e., [Cu(L1)2]n and [Cu(L2)2]n, in which both β-diketonate and pyridine groups are coordinated to the Cu(II) center, as well as in characterization of the mononuclear 1D complex Cu(L3)2, in which the central atom is bound only by two β-diketonate units.
Breeding Habitats of the Wood Thrush and Veery

scientific study. In very few cases, however, do we understand why a species occurs in a particular habitat, either in an ultimate (evolutionary) sense or a proximate (behavioral) sense. Before these questions can be adequately approached, a knowledge of the quantitative aspects of species' habitats is necessary. Most information on this subject comes from comparative habitat analyses (e.g., Sturman 1968, Wiens 1969, James 1971, Anderson and Shugart 1974). Vegetation structure has been emphasized heavily in such studies and often has been adequate to measure habitat differences among species. Floristic and physical factors have received less attention although MacArthur (1964) suggested that the latter may be important for at least some species. This study examines quantitatively the habitats of two woodland thrushes, the Wood Thrush (Hylocichla mustelina) and the Veety (Catharus fuscescens). My purposes were to determine how and to what extent their habitats differed and to indicate factors which seemed to be favored by each species in its selection of habitats. Some physical and floristic, as well as structural, habitat features were included to examine their effects on the
Indication of Electromagnetic Field Exposure via RBF-SVM Using Time-Series Features of Zebrafish Locomotion

This paper introduces a novel model based on support vector machine with radial basis function kernel (RBF-SVM) using time-series features of zebrafish (Danio rerio) locomotion exposed to different electromagnetic fields (EMFs) to indicate the corresponding EMF exposure. A group of 14 adult zebrafish was randomly divided into two groups, 7 in each group; the fish of each group have the novel tank test under a sham or real magnetic exposure of 6.78 MHz and about 1 A/m. Their locomotion in the tests was videotaped to convert into the x, y coordinate time-series of the trajectories for reforming time-series matrices according to different time-series lengths. The time-series features of zebrafish locomotion were calculated by the comparative time-series analyzing framework highly comparative time-series analysis (HCTSA), and a limited number of the time-series features that were most relevant to the EMF exposure conditions were selected using the minimum redundancy maximum relevance (mRMR) algorithm for RBF-SVM classification training. Before this, ambient environmental parameters (AEPs) had little effect on the locomotion performance of zebrafish processed by the empirical method, which had been quantitatively verified by regression using another group of 14 adult zebrafish. The results have demonstrated that the purposed model is capable of accurately indicating different EMF exposures. All classification accuracies can be 100%, and the classification precision of several classifiers based on specific parameters and feature sets with specific dimensions can reach higher than 95%. The speculative reason for this result is that the specified EMF has affected the zebrafish neural aspect, which is then reflected in their behaviors. The outcomes of this study have provided a new indication model for EMF exposures and provided a reference for the investigation of the impact of EMF exposure.
Long noncoding RNA PICSAR/miR‐588/EIF6 axis regulates tumorigenesis of hepatocellular carcinoma by activating PI3K/AKT/mTOR signaling pathway

Accumulating evidence has identified long noncoding RNAs (lncRNAs) as regulators in tumor progression and development. Here, we elucidated the function and possible molecular mechanisms of the effect of lncRNA‐PICSAR (p38 inhibited cutaneous squamous cell carcinoma associated lincRNA) on the biological behaviors of HCC. In the present study, we found that PICSAR was upregulated in HCC tissues and cells and correlated with progression and poor prognosis in HCC patients. Gain‐ and loss‐of‐function experiments indicated that PICSAR enhanced cell proliferation, colony formation, and cell cycle progression and inhibited apoptosis of HCC cells. PICSAR could function as a competing endogenous RNA by sponging microRNA (miR)‐588 in HCC cells. Mechanically, miR‐588 inhibited HCC progression and alternation of miR‐588 reversed the promotive effects of PICSAR on HCC cells. In addition, we confirmed that eukaryotic initiation factor 6 (EIF6) was a direct target of miR‐588 in HCC and mediated the biological effects of miR‐588 and PICSAR in HCC, resulting in PI3K/AKT/mTOR pathway activation. Our data identified PICSAR as a novel oncogenic lncRNA associated with malignant clinical outcomes in HCC patients. PICSAR played an oncogenic role by targeting miR‐588 and subsequently promoted EIF6 expression and PI3K/AKT/mTOR activation in HCC. Our results revealed that PICSAR could be a potential prognostic biomarker and therapeutic target for HCC.
Assignment of individuals to populations: Bayesian methods and multi-locus genotypes

Multi-locus genotype data have become a powerful management tool to identify degree of population structure, assign individuals to populations, identify hybridization, and assess rates of migration between populations. We use an example from the tuberculosis (TB)-infected white-tailed deer (Odocoileus virginianus) population in northeast Michigan (Deer Management Unit [DMU] 452) to illustrate the utility of multi-locus genotype data combined with assignment testing approaches in a wildlife management setting. We used microsatellite loci to establish that an unknown TB-positive deer tissue sample originated from a deer outside DMU452. Subsequently, we used multi-locus genotypic data and a likelihood-based assignment test to place TB-positive deer harvested outside of DMU452 into their populations of origin. We used genetic algorithms to identify a maximally informative subset of loci. We also determined estimates of statistical confidence in individual assignment decisions. Data indicate that the unknown TB-positive deer tissue sample was from a deer harvested outside DMU452. Two TB-positive deer killed outside of DMU452 were assigned to the local populations where they were harvested. The presence of TB-positive deer outside DMU452 suggests that TB is present in multiple areas of Michigan, dictating increased surveillance and changes in management policy for white-tailed deer throughout the state.
Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment

By binding to actin filaments, non-muscle myosin II (NMII) generates actomyosin networks that hold unique contractile properties. Their dynamic nature is essential for neuronal biology including the establishment of polarity, growth cone formation and motility, axon growth during development (and axon regeneration in the adult), radial and longitudinal axonal tension, and synapse formation and function. In this review, we discuss the current knowledge on the spatial distribution and function of the actomyosin cytoskeleton in different axonal compartments. We highlight some of the apparent contradictions and open questions in the field, including the role of NMII in the regulation of axon growth and regeneration, the possibility that NMII structural arrangement along the axon shaft may control both radial and longitudinal contractility, and the mechanism and functional purpose underlying NMII enrichment in the axon initial segment. With the advances in live cell imaging and super resolution microscopy, it is expected that in the near future the spatial distribution of NMII in the axon, and the mechanisms by which it participates in axonal biology will be further untangled.
Simulated larval connectivity among Australia’s southwest submarine canyons

: A biophysical dispersal model was used to simulate hydrodynamic connectivity among canyons located within Australia’s southwest marine region using ophiuroid (brittlestars) larvae as a model species. The results show that exchange among canyons in this area is greatly influenced by the Leeuwin Current, transporting larvae in a unidirectional manner around Cape Leeuwin, and continuing eastwards along the Great Australian Bight. Larvae within canyons tend to remain within them; however, if they are transported above the canyon walls, they then have the opportunity to be transported significant distances (thousands of km). Analysis of the variability in connectivity patterns reveals concentrated larval flow near the shelf break, with increasing levels of variability in larval flow leading offshore from the canyons. While the average potential larval flow distance and duration (unweighted by dispersal probability values) between canyons were approximately 550 km and 33 d, respectively, the average realized larval flow distance and duration (weighted by dispersal probability values) were approximately 20 km and 5 d, respec-tively. This study provides the first consideration of larval connectivity among submarine canyons and will help improve management of these features by providing a better understanding of larval movement, transboundary exchange and the potential spread of invasive species.
Discussion on “Testing small study effects in multivariate meta‐analysis” by Chuan Hong, Georgia Salanti, Sally Morton, Richard Riley, Haitao Chu, Stephen E. Kimmel and Yong Chen

The paper under discussion (described as “the paper” in this discussion) introduces models for the effect of small studies in multivariate meta analysis. This is done under the working hypothesis that this can be analyzed by studying the effect of the study-specific standard error on the outcomes of interest. That is fine with me. However, I am concerned about the way the data are modeled in the paper and the lack of transparency of the way the data are analyzed. In this discussion, I propose a modification of the model and a simplification of the analysis. I present a more detailed analysis of the two case studies. The P-values obtained in that analysis are close to the ones obtained in the paper. Finally, a sharper correction for multiple testing is presented and the lack of interpretation of “just P-values” is commented.
Perinatal Distress During COVID-19: Thematic Analysis of an Online Parenting Forum

Background The COVID-19 global pandemic has impacted the whole of society, requiring rapid implementation of individual-, population-, and system-level public health responses to contain and reduce the spread of infection. Women in the perinatal period (pregnant, birthing, and postpartum) have unique and timely needs for directives on health, safety, and risk aversion during periods of isolation and physical distancing for themselves, their child or children, and other family members. In addition, they are a vulnerable group at increased risk of psychological distress that may be exacerbated in the context of social support deprivation and a high-risk external environment. Objective The aim of this study is to examine the public discourse of a perinatal cohort to understand unmet health information and support needs, and the impacts on mothering identity and social dynamics in the context of COVID-19. Methods A leading Australian online support forum for women pre- through to postbirth was used to interrogate all posts related to COVID-19 from January 27 to May 12, 2020, inclusive. Key search terms included “COVID,” “corona,” and “pandemic. ” A three-phase analysis was conducted, including thematic analysis, sentiment analysis, and word frequency calculations. Results The search yielded 960 posts, of which 831 were included in our analysis. The qualitative thematic analysis demonstrated reasonable understanding, interpretation, and application of relevant restrictions in place, with five emerging themes identified. These were (1) heightened distress related to a high-risk external environment; (2) despair and anticipatory grief due to deprivation of social and family support, and bonding rituals; (3) altered family and support relationships; (4) guilt-tampered happiness; and (5) family future postponed. Sentiment analysis revealed that the content was predominantly negative (very negative: n=537 and moderately negative: n=443 compared to very positive: n=236 and moderately positive: n=340). Negative words were frequently used in the 831 posts with associated derivatives including “worried” (n=165, 19.9%), “risk” (n=143, 17.2%), “anxiety” (n=98, 11.8%), “concerns” (n=74, 8.8%), and “stress” (n=69, 8.3%). Conclusions Women in the perinatal period are uniquely impacted by the current pandemic. General information on COVID-19 safe behaviors did not meet the particular needs of this cohort. The lack of nuanced and timely information may exacerbate the risk of psychological and psychosocial distress in this vulnerable, high-risk group. State and federal public health departments need to provide a central repository of information that is targeted, consistent, accessible, timely, and reassuring. Compensatory social and emotional support should be considered, using alternative measures to mitigate the risk of mental health disorders in this cohort.
Redox Signaling and Regional Heterogeneity of Endothelial Dysfunction in db/db Mice

The variable nature of vascular dysfunction in diabetes is not well understood. We explored the functional adaptation of different arteries in db/db mice in relation to increased severity and duration of diabetes. We compared endothelium-dependent and -independent vasodilation in the aortae, as well as the carotid and femoral arteries, of db/db mice at three ages in parallel with increased body weight, oxidative stress, and deterioration of glycemic control. Vascular responses to in vitro generation of reactive oxygen species (ROS) and expression of superoxide dismutase (SOD) isoforms were assessed. There was a progressive impairment of endothelium-dependent and -independent vasorelaxation in the aortae of db/db mice. The carotid artery was resistant to the effects of in vivo and in vitro induced oxidative stress, and it maintained unaltered vasodilatory responses, likely because the carotid artery relaxed in response to ROS. The femoral artery was more reliant on dilation mediated by endothelium-dependent hyperpolarizing factor(s), which was reduced in db/db mice at the earliest age examined and did not deteriorate with age. Substantial heterogeneity exists between the three arteries in signaling pathways and protein expression of SODs under physiological and diabetic conditions. A better understanding of vascular heterogeneity will help develop novel therapeutic approaches for targeted vascular treatments, including blood vessel replacement.
Inadequate tissue mineralization promotes cancer cell attachment

Bone metastases are a frequent complication in prostate cancer, and several studies have shown that vitamin D deficiency promotes bone metastases. However, while many studies focus on vitamin D’s role in cell metabolism, the effect of chronically low vitamin D levels on bone tissue, i.e. insufficient mineralization of the tissue, has largely been ignored. To investigate, whether poor tissue mineralization promotes cancer cell attachment, we used a fluorescence based adhesion assay and single cell force spectroscopy to quantify the adhesion of two prostate cancer cell lines to well-mineralized and demineralized dentin, serving as biomimetic bone model system. Adhesion rates of bone metastases-derived PC3 cells increased significantly on demineralized dentin. Additionally, on mineralized dentin, PC3 cells adhered mainly via membrane anchored surface receptors, while on demineralized dentin, they adhered via cytoskeleton-anchored transmembrane receptors, pointing to an interaction via exposed collagen fibrils. The adhesion rate of lymph node derived LNCaP cells on the other hand is significantly lower than that of PC3 and not predominately mediated by cytoskeleton-linked receptors. This indicates that poor tissue mineralization facilitates the adhesion of invasive cancer cells by the exposure of collagen and emphasizes the disease modifying effect of sufficient vitamin D for cancer patients.
Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

Tissue engineering has recently emerged as a novel strategy for the regeneration of damaged skeletal muscle tissues due to its ability to regenerate tissue. However, tissue engineering is challenging due to the need for state-of-the-art interdisciplinary studies involving material science, biochemistry, and mechanical engineering. For this reason, electrospinning and three-dimensional (3D) printing methods have been widely studied because they can insert embedded muscle cells into an extracellular-matrix-mimicking microenvironment, which helps the growth of seeded or laden cells and cell signals by modulating cell–cell interaction and cell–matrix interaction. In this mini review, the recent research trends in scaffold fabrication for skeletal muscle tissue regeneration using advanced techniques, such as electrospinning and 3D bioprinting, are summarized. In conclusion, the further development of skeletal muscle tissue engineering techniques may provide innovative results with clinical potential for skeletal muscle regeneration.
Prevalence of Mental Disorders in Abakaliki, Ebonyi State, Southeastern Nigeria

Background: Increasing psychological stressors have posed challenges to the well-being of the people across the globe and greatly affected the functionality and economic output of the individuals and the society. Nigeria has no existing mental health registry. Data on the prevalence of mental disorders are not readily available owing to lack of mental health registry. Hence, this study assessed the prevalence of mental disorders in Abakaliki metropolis, Ebonyi State. Aim: To determine the prevalence of mental disorders and associated factors among the residents of Abakaliki metropolis, Ebonyi State. Method: This cross-sectional descriptive research study involved 400 participants. Questionnaires adapted from world mental health diagnostic interview and General Health Questionnaire 12 were used for data collection. Data were analyzed using descriptive statistics and hypotheses tested using chi-square test at significance level of . 05. Results: The prevalence of mental disorders among the respondents was 70% depressive disorders and 52.3% substance use disorders—tranquilizers (34.9%) and stimulants (15.8%) were the commonly used, while 85.3% suffered anxiety disorders. These were common among age range of 19 to 28 years—those with higher education and the unemployed. More females had mental disorders except substance use disorders, which was higher in males (53.4%). There was no significant relationship between mental disorders and the demographic variables, but significant relationship was found to exist between individuals’ age and anxiety disorder. Conclusion: Mental disorders, such as depression, anxiety, and substance use disorders, are common among the respondents and, therefore, calls for urgent attention of the government to improve the mental health of the people.
Sex Differences in Salience Network Connectivity and its Relationship to Sensory Over‐Responsivity in Youth with Autism Spectrum Disorder

Individuals with autism spectrum disorder (ASD) are significantly more likely to experience sensory over‐responsivity (SOR) compared to neurotypical controls. SOR in autism has been shown to be related to atypical functional connectivity in the salience network (SN), a brain network thought to help direct attention to the most relevant stimuli in one's environment. However, all studies to date which have examined the neurobiological basis of sensory processing in ASD have used primarily male samples so little is known about sex differences in the neural processing of sensory information. This study examined the relationship between SOR and resting‐state functional connectivity in the SN for 37 males and 16 females with autism, ages 8–17 years. While there were no sex differences in parent‐rated SOR symptoms, there were significant sex differences in how SOR related to SN connectivity. Relative to females with ASD, males with ASD showed a stronger association between SOR and increased connectivity between the salience and primary sensory networks, suggesting increased allocation to sensory information. Conversely, for females with ASD, SOR was more strongly related to increased connectivity between the SN and prefrontal cortex. Results suggest that the underlying mechanisms of SOR in ASD are sex specific, providing insight into the differences seen in the diagnosis rate and symptom profiles of males and females with ASD.