Miracle-dz/UniFAR-FTUs huggingface dataset:
Neonates With Urinary Tract Infection

Supplemental Digital Content is available in the text. Background: Our objectives were to determine the prevalence of and to identify risk factors for coexisting bacterial meningitis (BM) in neonates with urinary tract infection (UTI). Methods: A cross-sectional study was conducted at pediatric emergency department of a tertiary teaching hospital from 2001 to 2017. Infants <29 days of age with UTI (≥10,000 colony-forming units/mL of a single pathogen from a catheterized specimen in association with positive urinalysis) were included. Definite BM was defined as growth of a single bacterial pathogen from a cerebrospinal fluid (CSF) sample and probable BM as (1) positive blood culture with CSF pleocytosis and treatment consistent with BM or (2) antibiotic pretreatment before lumbar puncture, CSF pleocytosis and treatment consistent with BM. Univariate testing was used to identify possible risk factors associated with BM. Receiver operating characteristics curves were constructed for the laboratory markers associated with BM. Results: Three hundred seventy-one infants were included. Five [1.3%; 95% confidence interval (CI): 0.6%–3.1%] had BM: 4 definite BM and 1 probable BM. Risk factors detected for BM were classified as not being well-appearing and a procalcitonin value ≥0.35 ng/mL [sensitivity of 100% (95% CI: 56.6%–100%) and negative predictive value of 100% (95% CI: 96.1%–100%)]. Conclusions: Coexisting BM occurs uncommonly in neonates with UTI. Well-appearing neonates with UTI and procalcitonin value <0.35 ng/mL were at very low risk for BM; avoiding routine lumbar puncture in these patients should be considered.
Whole blood transfusion versus component therapy in adult trauma patients with acute major haemorrhage

Objective In the era of damage control resuscitation of trauma patients with acute major haemorrhage, transfusion practice has evolved to blood component (component therapy) administered in a ratio that closely approximates whole blood (WB). However, there is a paucity of evidence supporting the optimal transfusion strategy in these patients. The primary objective was therefore to establish if there is an improvement in survival at 30 days with the use of WB transfusion compared with blood component therapy in adult trauma patients with acute major haemorrhage. Methodology A systematic literature search was performed on 15 December 2019 to identify studies comparing WB transfusion with component therapy in adult trauma patients and mortality at 30 days. Studies which did not report mortality were excluded. Methodological quality of included studies was interpreted using the Cochrane risk of bias tool, and rated using the Grading of Recommendations Assessment, Development and Evaluation approach. Results Search of the databases identified 1885 records, and six studies met the inclusion criteria involving 3255 patients. Of the three studies reporting 30-day mortality (one randomised controlled trial (moderate evidence) and two retrospective (low and very low evidence, respectively)), only one study demonstrated a statistically significant difference between WB and component therapy, and two found no statistical difference. Two retrospective studies reporting in-hospital mortality found no statistical difference in unadjusted mortality, but both reported statistically significant logistic regression analyses demonstrating that those with a WB transfusion strategy were less likely to die. Conclusion Recognising the limitations of this systematic review relating to the poor-quality evidence and limited number of included trials, it does not provide evidence to support or reject use of WB transfusion compared with component therapy for adult trauma patients with acute major haemorrhage. PROSPERO registration number CRD42019131406.
Towards a digital African Flora

Modern approaches to Flora writing are increasingly being built around database technology. Databases allow for great flexibility in the range of questions that can be asked of the Flora, and consequently greatly enhance the utility of the Flora in the core roles of identification of species and nomenclatural queries. There are, how ever, few examples where existing floristic works have been converted into database format, so that much of our legacy of floristic texts still rests in the form of the printed page. In this paper we address the problem of the digitisation of legacy texts (see www.kew.org/efloras/). We describe a method that we used to create an online database of the Flora Zambesiaca for identification and nomenclatural queries. We assert that the method we used can be generalised to cope with most standard taxonomic formats. We conclude that on a cost benefit basis Flora digitisation compares very favourably to other digitisation approaches, such as specimen databasing and interactive key construction, and could be used with good effect to direct digitisation in these other areas.
Biomaterials in Neurodegenerative Disorders: A Promising Therapeutic Approach

Neurodegenerative disorders (i.e., Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, and spinal cord injury) represent a great problem worldwide and are becoming prevalent because of the increasing average age of the population. Despite many studies having focused on their etiopathology, the exact cause of these diseases is still unknown and until now, there are only symptomatic treatments. Biomaterials have become important not only for the study of disease pathogenesis, but also for their application in regenerative medicine. The great advantages provided by biomaterials are their ability to mimic the environment of the extracellular matrix and to allow the growth of different types of cells. Biomaterials can be used as supporting material for cell proliferation to be transplanted and as vectors to deliver many active molecules for the treatments of neurodegenerative disorders. In this review, we aim to report the potentiality of biomaterials (i.e., hydrogels, nanoparticles, self-assembling peptides, nanofibers and carbon-based nanomaterials) by analyzing their use in the regeneration of neural and glial cells their role in axon outgrowth. Although further studies are needed for their use in humans, the promising results obtained by several groups leads us to suppose that biomaterials represent a potential therapeutic approach for the treatments of neurodegenerative disorders.
Populations of soil organisms under continuous set stocked and high intensity‐short duration rotational grazing practices in the central tablelands of New South Wales (Australia)

Abstract Grazing management practices modify soil structure, which, in turn, alters the behaviour and population dynamics of soil‐inhabiting organisms. Monitoring of changes in abundance and richness of soil organisms is vital in determining the sustainability of grazing regimes. In this paper, populations of soil organisms were compared—using abundance and population trends as an indicator of diversity—in two different grazing practices (a) conventional, set stocked (SS) grazing regime and (b) high intensity‐short duration (HI‐SD) grazing regime) and an ungrazed control (C). A thorough sampling of earthworms and arthropods, and an assay of soil microbial biomass and respiration was made in Spring 2004 (September‐November), after a pilot sampling of earthworms and arthropods in Autumn 2004 (March‐May). Earthworm numbers were found to be unaffected by grazing regimes, however, microarthropod abundance at 0–10 cm soil depth was significantly higher in soil of the HI‐SD grazing regime and in the ungrazed control, when compared with set stocking. Microbial biomass and respiration did not differ across treatments. Overall, our results indicate that arthropod abundance reflects the changes impacting on soil structure as a consequence of grazing practice. Evaluation of earthworm populations was not found to be useful in discriminating between the two grazing regimes, because the earthworm numbers were principally affected by rainfall.
Psychophysiological Responses During a Cycling Test to Exhaustion While Wearing the Elevation Training Mask

Abstract López-Pérez, ME, Romero-Arenas, S, Colomer-Poveda, D, Keller, M, and Márquez, G. Psychophysiological responses during a cycling test to exhaustion while wearing the elevation training mask. J Strength Cond Res 36(5): 1282–1289, 2022—The aim of this study was to investigate the psychophysiological effects of wearing the elevation training mask (ETM). Twelve men performed time-to-exhaustion (TTE) tests at 75% of peak power output with and without wearing the ETM. Heart rate (HR), rating of perceived exertion (RPE), breathing discomfort (BD), and oxygen saturation (SpO2) were measured during the TTE. Prefrontal cortex (PFC) and vastus lateralis oxygenated, deoxygenated, and total hemoglobin were monitored using near-infrared spectroscopy. At the end of each test, blood lactate values (La-) were collected, and subjects completed the Beck Anxiety Inventory (BAI). The mask caused a reduction in the TTE (−37.7%; p < 0.001) and in the SpO2 (−2%; p < 0.001). Beck Anxiety Inventory scores were negatively correlated with the changes observed in the TTE (r = −0.77; p < 0.01). La-, HR, and muscle oxygenation displayed similar results across conditions. In conjunction with an increased hemodynamic response in the PFC, subjects reported higher RPE and BD values in the ETM condition (p < 0.01). Finally, BAI scores were negatively correlated with the changes observed in the TTE (r = −0.77; p < 0.01). This study suggests that wearing the ETM induces psychophysiological alterations affecting the exercise tolerance and limiting the performance.
DXO/Rai1 enzymes remove 5′-end FAD and dephospho-CoA caps on RNAs

Abstract In eukaryotes, the DXO/Rai1 enzymes can eliminate most of the incomplete and non-canonical NAD caps through their decapping, deNADding and pyrophosphohydrolase activities. Here, we report that these enzymes can also remove FAD and dephospho-CoA (dpCoA) non-canonical caps from RNA, and we have named these activities deFADding and deCoAping. The crystal structures of mammalian DXO with 3′-FADP or CoA and fission yeast Rai1 with 3′-FADP provide elegant insight to these activities. FAD and CoA are accommodated in the DXO/Rai1 active site by adopting folded conformations. The flavin of FAD and the pantetheine group of CoA contact the same region at the bottom of the active site tunnel, which undergoes conformational changes to accommodate the different cap moieties. We have developed FAD-capQ to detect and quantify FAD-capped RNAs and determined that FAD caps are present on short RNAs (with less than ∼200 nucleotides) in human cells and that these RNAs are stabilized in the absence of DXO.
Physician networks and potentially inappropriate opioid prescriptions

Abstract Background: Opioid overdose is a national health priority and curbing inappropriate prescribing is critical. In 2016, the Centers for Disease Control and Prevention (CDC) issued appropriate prescribing guidelines. Objectives: Examine associations between care networks defined by shared patients and problematic opioid prescribing. Methods: Analysis was at the provider-year level. Social network analysis (SNA) applied to the Medicaid MarketScan® Research Database for the years 2010–2015 identified care communities, each community’s level of integration (centralization), and each provider’s integration (centrality). Nested multivariable logistic regressions controlling for patient mix and provider specialty simultaneously examined the risk of any (incident) and repeated (prevalent) inappropriate prescribing. Outcomes: Four behaviors defined by the CDC guidelines were examined: (1) more than 90 days continuous supply of high-dose opioid analgesics for chronic pain, (2) overlapping opioid supplies, (3) overlapping opioid and benzodiazepine prescriptions, and (4) prescribing an extended release opioid for an acute pain diagnosis. Results: Provider centrality was associated with reduced incidence of outcome (2) (OR: 0.95) and decreased prevalence of outcomes (1), (2), and (3). However, higher incidence (OR: 1.32) and prevalence (OR: 1.027) of outcome (4) were observed. Conversely, centralization associated with decreased incidence of (1) and (2) and lower prevalence of (1), (2), and (3). Conclusions: Greater provider integration is associated with a lower risk of a provider’s patients repeatedly having potentially inappropriate prescription fills; however, the association with a provider having any potentially problematic prescription is more ambiguous.
A Cytokinin Signaling Type-B Response Regulator Transcription Factor Acting in Early Nodulation1

A Medicago truncatula cytokinin-signaling transcription factor is required for early symbiotic nodulation and to regulate key Nod factor signaling and endoreduplication genes. Nitrogen-fixing root nodulation in legumes challenged with nitrogen-limiting conditions requires infection of the root hairs by soil symbiotic bacteria, collectively referred to as rhizobia, and the initiation of cell divisions in the root cortex. Cytokinin hormones are critical for early nodulation to coordinate root nodule organogenesis and the progression of bacterial infections. Cytokinin signaling involves regulation of the expression of cytokinin primary response genes by type-B response regulator (RRB) transcription factors. RNA interference or mutation of MtRRB3, the RRB-encoding gene most strongly expressed in Medicago truncatula roots and nodules, significantly decreased the number of nodules formed, indicating a function of this RRB in nodulation initiation. Fewer infection events were also observed in rrb3 mutant roots associated with a reduced Nod factor induction of the Early Nodulin 11 (MtENOD11) infection marker, and of the cytokinin-regulated Nodulation Signaling Pathway 2 (MtNSP2) gene. Rhizobial infections correlate with an expansion of the nuclear area, suggesting the activation of endoreduplication cycles linked to the cytokinin-regulated Cell Cycle Switch 52A (MtCCS52A) gene. Although no significant difference in nucleus size and endoreduplication were detected in rhizobia-infected rrb3 mutant roots, expression of the MtCCS52A endoreduplication marker was reduced. As the MtRRB3 expression pattern overlaps with those of MtNSP2 and MtCCS52A in roots and nodule primordia, chromatin immunoprecipitation-quantitative PCR and protoplast trans-activation assays were used to show that MtRRB3 can interact with and trans-activate MtNSP2 and MtCCS52A promoters. Overall, we highlight that the MtRRB3 cytokinin signaling transcription factor coordinates the expression of key early nodulation genes.
Melatonin prevents neuroinflammation and relieves depression by attenuating autophagy impairment through FOXO3a regulation.

Major depressive disorder (MDD) is a life-threatening illness characterized by mood changes and high rates of suicide. Although the role of neuroinflammation in MMD has been studied, the mechanistic interplay between antidepressants, neuroinflammation, and autophagy is yet to be investigated. The present study investigated the effect of melatonin on LPS-induced neuroinflammation, depression, and autophagy impairment. Our results showed that in mice, lipopolysaccharide (LPS) treatment induced depressive-like behaviors and caused autophagy impairment by dysregulating ATG genes. Moreover, LPS-treatment significantly increased the levels of cytokines (TNFα, IL-1β, IL-6), enhanced NF-ᴋB phosphorylation, caused glial (astrocytes and microglia) cell activation, dysregulated FOXO3a expression, increased the levels of redox signaling molecules such as ROS/TBARs, and altered expression of Nrf2, SOD2, and HO-1. Melatonin treatment significantly abolished the effects of LPS, as demonstrated by improved depressive-like behaviors, normalized autophagy-related gene expression, and reduced levels of cytokines. Further, we investigated the role of autophagy in LPS-induced depressive-like behavior and neuroinflammation using autophagy inhibitors 3-MA and Ly294002. Interestingly, inhibitors treatment significantly abolished and reversed the anti-depressive, pro-autophagy and anti-inflammatory effects of melatonin. The present study concludes that the anti-depressive effects of melatonin in LPS-induced depression might be mediated via autophagy modulation through FOXO3a signaling.
Inhibition of SARS-CoV-2 Infection by the Cyclophilin Inhibitor Alisporivir (Debio 025)

Cyclophilins play a key role in the life cycle of coronaviruses. Alisporivir (Debio 025) is a nonimmunosuppressive analogue of cyclosporine with potent cyclophilin inhibition properties. Alisporivir reduced SARS-CoV-2 RNA production in a dose-dependent manner in Vero E6 cells, with a 50% effective concentration (EC50) of 0.46 ± 0.04 μM. Alisporivir inhibited a postentry step of the SARS-CoV-2 life cycle. These results justify rapidly conducting a proof-of-concept phase 2 trial with alisporivir in patients with SARS-CoV-2 infection. ABSTRACT Cyclophilins play a key role in the life cycle of coronaviruses. Alisporivir (Debio 025) is a nonimmunosuppressive analogue of cyclosporine with potent cyclophilin inhibition properties. Alisporivir reduced SARS-CoV-2 RNA production in a dose-dependent manner in Vero E6 cells, with a 50% effective concentration (EC50) of 0.46 ± 0.04 μM. Alisporivir inhibited a postentry step of the SARS-CoV-2 life cycle. These results justify rapidly conducting a proof-of-concept phase 2 trial with alisporivir in patients with SARS-CoV-2 infection.
A Prototype Microwave System for 3D Brain Stroke Imaging

This work focuses on brain stroke imaging via microwave technology. In particular, the open issue of monitoring patients after stroke onset is addressed here in order to provide clinicians with a tool to control the effectiveness of administered therapies during the follow-up period. In this paper, a novel prototype is presented and characterized. The device is based on a low-complexity architecture which makes use of a minimum number of properly positioned and designed antennas placed on a helmet. It exploits a differential imaging approach and provides 3D images of the stroke. Preliminary experiments involving a 3D phantom filled with brain tissue-mimicking liquid confirm the potential of the technology in imaging a spherical target mimicking a stroke of a radius equal to 1.25 cm.
Emerging from the Shadows: Intrinsic and Extrinsic Factors Facing Community Health Workers in Western Cape, South Africa

Community health workers (CHWs) have been identified as a key component of the health workforce in South Africa. However, the efficacy of CHW programs continues to be limited by a poor understanding of facilitators and barriers to CHW engagement. This study explores intrinsic and extrinsic factors that CHWs face. We conducted in-depth interviews with 20 CHWs in order to understand the challenges they may face as they implement their duties linked to the primary health care strategy in the Western Cape, South Africa. All interviews were audiotaped, transcribed verbatim, coded and analyzed using NVivo 12. Drawing on narratives of CHWs, we illustrate the complex issues surrounding CHW outreach in poor rural communities. The CHWs identified five key areas of challenges with respect to personal health, gender issues, poor community understanding of CHWs roles, environmental challenges and lack of patient adherence. These all hinder the ability of CHWs to meet their personal and familial needs, as well as those of the community members they support. There is a need to address the intrinsic needs of CHWs in order to ensure their emotional and physical well-being, as well as a need to create an awareness of the roles of CHWs.
Wing wear is a poor estimate of age in Cerceris fumipennis (Hymenoptera, Crabronidae)

The native, ground nesting, beetle hunting philanthine wasp Cerceris fumipennis Say is in development as a biosurveillance tool to detect Emerald Ash Borer (Agrilus planipennis) and other pest Buprestidae threatening the forests of North America (Marshall et al. 2005; Careless and Marshall 2010; Nalepa and Swink 2011). As part of that effort the North Carolina (NC) Department of Agriculture & Consumer Services has been working to determine the phenology and life history of C. fumipennis in the state, primarily to determine its activity overlap with prey species of interest. An examination of specimens in the NC State University Insect Museum indicated that C. fumipennis had been collected in Wake County, NC, as early as 24 May and as late as 22 September between 1921 and 1982, and Kurczewski and Miller (1984) noted that C. fumipennis probably has a partial second brood in the south. Observations of wing wear of C. fumipennis females in Wake County during 2009 suggested that there may be either an extended emergence of a single generation, or that more than one generation per year occurs in the state. During the third week of July, females with completely intact wings as well as females with severely damaged wing margins were both present in the same nesting aggregation. In 2010 a study was conducted at that aggregation, a baseball diamond in the Raleigh City Parks system, to determine if wing wear in C. fumipennis may be positively correlated with female age. Shortly after nests began appearing at the site (30 May), females were captured and given a unique mark on the thorax using DecoColor opaque paint markers (Fig. 1a). A picture of each marked female’s right wing was taken with a Sony Cybershot JHR 24: 43–46 (2012)
Evidence against a role for phytochelatins in naturally selected increased cadmium tolerance in Silene vulgaris (Moench) Garcke

summary The production of phytochelatins in roots of both cadmium–sensitive and cadmium–tolerant plants of Silene vulgaris (Moench) Garcke in response to Cd supply was recorded over a 7–day growth period. At the same level of Cd, sensitive plants produced more phytochelatins than tolerant ones. In sensitive roots this higher production of PCs was associated with a higher Cd content within one day. From the third day, however, Cd contents were similar, but the difference in PC production persisted. Exposure to buthionine sulphoximine strongly inhibited the production of phytochelatins in roots during cadmium treatment in both sensitive and tolerant plants, but only affected root growth of sensitive plants. Sensitive plants exhibited a higher Cd uptake, which was manifested in a higher Cd accumulation in the shoot. It is concluded that differential cadmium tolerance mechanism in Silene vulgaris is not due to differential production of phytochelatins.