Miracle-dz/UniFAR-FTUs huggingface dataset:
Tumor Necrosis Factor-Like Weak Inducer of Apoptosis (TWEAK) Enhances Activation of STAT3/NLRC4 Inflammasome Signaling Axis through PKCδ in Astrocytes: Implications for Parkinson’s Disease

Astrocytic dysfunction has been implicated in Parkinson’s disease (PD) pathogenesis. While the Tumor necrosis factor-like weak inducer of apoptosis (TWEAK)/Fn14 signaling axis is known to play a role in PD-like neuropathology, the molecular mechanisms that govern this process remain poorly understood. Herein, we show that TWEAK levels are elevated in PD serum compared to controls. Moreover, using both U373 human astrocyte cells and primary mouse astrocytes, we demonstrate that TWEAK induces mitochondrial oxidative stress as well as protein kinase C delta (PKCδ) and signal transducer and activator of transcription 3 (STAT3) activation, accompanied by NLRC4 inflammasome activation and upregulation and release of proinflammatory cytokines, including IL-1β, TNF-α, and IL-18. Mechanistically, TWEAK-induced PKCδ activation enhances the STAT3/NLRC4 signaling pathway and other proinflammatory mediators through a mitochondrial oxidative stress-dependent mechanism. We further show that PKCδ knockdown and mito-apocynin, a mitochondrial antioxidant, suppress TWEAK-induced proinflammatory NLRC4/STAT3 signaling and cellular oxidative stress response. Notably, we validated our in vitro findings in an MPTP mouse model of PD and in mice receiving intrastriatal administration of TWEAK. These results indicate that TWEAK is a key regulator of astroglial reactivity and illustrate a novel mechanism by which mitochondrial oxidative stress may influence dopaminergic neuronal survival in PD.
Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Research on biologically active compounds has been increased in order to improve plant protection against various environmental stresses. Among natural sources, plants are the fundamental material for studying these bioactive compounds as their immune system consists of many peptides, proteins, and hormones. Osmotin is a multifunctional stress-responsive protein belonging to pathogenesis-related 5 (PR-5) defense-related protein family, which is involved in inducing osmo-tolerance in plants. In this scenario, the accumulation of osmotin initiates abiotic and biotic signal transductions. These proteins work as antifungal agents against a broad range of fungal species by increasing plasma membrane permeability and dissipating the membrane potential of infecting fungi. Therefore, overexpression of tobacco osmotin protein in transgenic plants protects them from different stresses by reducing reactive oxygen species (ROS) production, limiting lipid peroxidation, initiating programmed cell death (PCD), and increasing proline content and scavenging enzyme activity. Other than osmotin, its homologous proteins, osmotin-like proteins (OLPs), also have dual function in plant defense against osmotic stress and have strong antifungal activity.
Effect of Age on the Performance of Laying Hens During the First Year of Production

Abstract Two hundred and seventy White Leghorn hens were used in this study. The experimental period commenced at 23 weeks of age and continued for 48 weeks. A daily light period of 14 hours was consistent for the whole experimental period. The individual hens were fed ad libitum with one of two isocaloric diets, containing 15 or 17% protein. Each feeding space provided the same level of environmental complexity and the feeder outline intentionally did not prevent visual contact across adjacent cages or cage rows and therefore, did not prevent the social facilitation of feeding responses. The effect of age during the laying season significantly influenced feed conversion, feed consumption and the weights of fresh and freeze dried egg components. Moisture contents of fresh yolk and fresh albumen did not exhibit parallel changes with age. Diets significantly affected egg production (73.2% vs. 71.9%), average daily egg mass production (46.65 g. vs. 46.30 g.), and the weight of freeze dried albumen (4.30 g. vs. 4.37 g.) for the 15 and 17% protein diets, respectively. The only significant interaction between diets and age were detected for egg mass. The distribution of individual feed conversion ratios and egg shell deformation deviated from normality both being positively skewed and kurtotic.
The protective effect of rutin against busulfan-induced testicular damage in adult rats

Abstract Here, we studied the protective effect of rutin (RUT) against testicular damage caused by busulfan (BUS) in rats. Adult male Wistar rats were intraperitoneally injected with BUS (4 mg/kg body weight at day 7 and 14), and then treated with RUT (30 mg/kg body weight) by gavage thrice weekly for 60 days. The results showed that BUS-induced increase in 3β-hydroxysteroid dehydrogenase (3β-HSD) was significantly decreased by RUT, whereas 17β-HSD activity and plasma testosterone concentration remained unaffected (p > 0.05). It was also observed that RUT inhibited BUS-induced increase in nitrite concentrations and myeloperoxidase enzyme activities in the plasma and testes (p < 0.05). Similarly, BUS-induced decrease in glutathione and increase in malondialdehyde concentrations in the testes were significantly normalized to control values by RUT. Finally, RUT administration showed some tendency to improve the architecture of the seminiferous epithelium of the rat’s testes after BUS treatment. Overall, RUT inhibited BUS-induced oxidative damage and inflammation in the testis of an experimental rat model.
Discriminating the Role of Photosynthetic and Heterotrophic Microbes Triggering Low-Mg Calcite Precipitation in Freshwater Biofilms (Lake Geneva, Switzerland)

Field and laboratory experiments in freshwater Lake Geneva (Switzerland) have recently demonstrated a substantial role of biofilms triggering the formation of ooids. However, the precise function and specificity of the microbes involved in this process remained elusive. In this study we compared biofilms lining ooid depressions in the natural environment with those grown under laboratory conditions in a BG11 media. Confocal microscopy, SEM and TEM as well as PCR-DGGE results have been used to accomplish this comparison at both microscopical and geochemical levels, respectively. Carbonate precipitates in the natural environment show that cyanobacteria dominate the microbial community in association with green algae and diatoms all embedded within the EPS. Analogously, low-Mg calcite crystals were formed in the laboratory experiments under light conditions. These crystals are found within the EPS and always associated with photosynthetic microorganisms. The latter in concert with the lack of evidence for the presence of sulfate-reducers, allow us to postulate that photosynthetic activity through increasing pH in EPS is the main factor triggering low-Mg calcite precipitation in Lake Geneva.
Optimization of callus induction and regeneration system for Pakistani wheat cultivars Kohsar and Khyber-87

Wheat is a member of family Poaceae. It is the major staple food of Pakistan. The present study was done to improve the regeneration of two commercially grown wheat varieties Kohsar and Khyber-87. Mature embryos were used as explants. Five different concentrations of 2,4-D; 2, 2.5, 3, 3.5 and 4 mg/L were used for callus induction. For regeneration, initially different concentrations (0.1 to 0.2) of IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine) were experimented. The best combination of these hormones that is, 0.1 mg/L IAA and 0.5 mg/L BAP were further subjected to experimentation along with different concentrations of kinetin; 0.1, 0.2, 0.3, 0.4, 0.5 and 1 mg/L. Maximum calli of Kohsar (83.3%) was obtained at 3 mg/L 2,4-D whereas for Khyber-87 maximum callus induction (71.70%) was obtained at 3.5 mg/l 2,4-D. The maximum regeneration of both Kohsar and Khyber-87 (80.5 and 62.2%, respectively) were obtained at the combinations of 0.1 mg/L IAA, 0.5 mg/L BAP and 0.5 mg/L kinetin.
A stochastic model for error correction of kinetochore-microtubule attachments in budding yeast

To divide replicated chromosomes equally between daughter cells, kinetochores must attach to microtubules emanating from opposite poles of the mitotic spindle (biorientation). An error correction mechanism facilitates this process by destabilizing erroneous kinetochore-microtubule attachments. Here we present a stochastic model of kinetochore-microtubule attachments, via an essential protein Ndc80 in budding yeast, Saccharomyces cerevisiae. Using the model, we calculate the stochastic dynamics of a pair of sister kinetochores as they transition among different attachment states. First of all, we determine the kinase-to-phosphatase balance point that maximizes the probability of biorientation, while starting from an erroneous attachment state. We find that the balance point is sensitive to the rates of microtubule-Ndc80 dissociation and derive an approximate analytical formula that defines the balance point. Secondly, we determine the probability of transition from low-tension amphitelic to monotelic attachment and find that, despite this probability being approximately 33%, biorientation can be achieved with high probability. Thirdly, we calculate the contribution of the geometrical orientation of sister kinetochores to the probability of biorientation and show that, in the absence of geometrical orientation, the biorientation error rate is much larger than that observed in experiments. Finally, we study the coupling of the error correction mechanism to the spindle assembly checkpoint by calculating the average binding of checkpoint-related proteins to the kinetochore during the error correction process.
Impact of Ultrasound Treatment and pH-Shifting on Physicochemical Properties of Protein-Enriched Barley Fraction and Barley Protein Isolate

Ultrasonication alone or in combination with a pH-shifting method could be applied as means for improving the techno-functional properties and performance of barley protein ingredients in liquid food matrix. Ultrasound technology was utilised with and without pH-shifting to 3, 7 and 9 aiming at investigating their impact on primary protein structure, protein solubility, particle size and colloidal stability of an air-classified protein-enriched barley fraction and a barley protein isolate. Shifting the pH of sample dispersion to 9 followed by neutralisation to pH 7 improved protein solubility and colloidal stability of the isolate whereas it had less impact on the protein-enriched fraction. Ultrasound treatment improved both protein solubility and colloidal stability of the protein-enriched fraction at alkaline pH and particle size reduction by ultrasonication was observed at all the studied pH-values. For protein isolate, ultrasonication improved protein solubility at all pH-values and colloidal stability was improved at acidic and neutral pH whereas the sample was inherently stable at alkaline pH. The protein profiles of both ingredients remained unaffected by ultrasound treatment. The results suggest adopting ultrasonication as a promising tool for improving applicability of barley protein ingredients in liquid food systems.
ELECTRON MICROSCOPE STUDIES OF SPERMATOZOA OF RHYNCHOSCIARA SP

The flagellar complex of the unusual motile spermatozoon of the fungus gnat, Rhynchosciara sp, does not conform to the usual "9 + 2" filament pattern but rather consists of over 350 pairs of filaments (doublet microtubules) distributed in a spiral array. Experiments were designed to disrupt and extract flagellar microtubular components from spermatozoa of the fungus gnat. Pepsin, chymotrypsin, potassium iodide, urea, and heat were used to extract specific portions of microtubule walls Such experiments provide information on the composition of the wall and the existence of wall sites selectively sensitive to various treatments Results obtained include: (a) doublet microtubules are comprised at least in part of protein, and all subunits are probably not identical; (b) a portion of the B subfiber is apparently more sensitive to disruption than other portions of the doublet microtubule; and (c) the ac cessory singlet microtubules may be chemically different from the doublet microtubules
Adaptation and transcultural translation into Spanish of the Patient‐Rated Tennis Elbow Evaluation Questionnaire

The purpose of this study was to perform the translation and cross‐cultural adaptation of the Patient‐Rated Tennis Elbow Evaluation Questionnaire to Spanish language and evaluate its reliability and validity. The translation and cultural adaptation into Spanish was done in accordance with the published guidelines. One‐hundred fifty Spanish‐speaking patients with unilateral chronic lateral epicondylalgia competed the questionnaire. Test‐retest reliability was established by the intraclass correlation coefficient. Internal consistency was established with Cronbach's α. To establish convergent validity, we used the Disabilities of the Arm, Shoulder, and Hand Questionnaire using the Spearman's correlation coefficient. Error estimation in the measurements was calculated with the standard error of measurement. Our results showed a high internal consistency (Cronbach's α = .96) and high test‐retest reliability (intraclass coefficient = .9; .89‐.94; P < .001). The Spearman's correlation coefficient (r = .765; P < .001) showed a good relationship between the Spanish version of the Patient‐Rated Tennis Elbow Evaluation Questionnaire and the Disabilities of the Arm, Shoulder, and Hand Questionnaire. The standard error of measurement (11.9%) showed little variability of measurements. In conclusion, the Spanish version of the Patient‐Rated Tennis Elbow Evaluation Questionnaire is a valid and reliable tool that can be used to assess lateral epicondylalgia in Spanish‐speaking individuals in order to implement the best treatment and reduce time with pain and disability.
FOOD-PLANTS AND DISTRIBUTION OF THE SPECIES OF CALLIGRAPHA IN CANADA, WITH DESCRIPTIONS OF NEW SPECIES (COLEOPTERA, CHRYSOMELIDAE)

Twenty-nine species and named subspecies of Calligrapha Chev. , including eight species previously undescribed, are listed below with notes on food-plant, distribution, and variability. New names are offered for the hamonyms elegans (Olivier) and similis (Rogers). The records and notes, except those credited to authors, are based almost entirely on material in the Canadian National Collection.
Prognostic significance of body mass index‐adjusted criteria for left ventricular hypertrophy

Electrocardiographic left ventricular hypertrophy (ECG‐LVH) is associated with both cardiovascular and all‐cause mortality. Obesity attenuates the sensitivity of several ECG‐LVH criteria, so body mass index (BMI) adjusted criteria have been developed. However, the prognostic significance of BMI‐adjusted ECG‐LVH criteria is not known. This analysis included 7812 participants (59.8 ± 13.4 years, 53% women, 50% non‐Hispanic‐whites) from the Third National Health and Nutrition Examination Survey. The Cornell criteria (R in aVL + S in V3 ≥ 2800 µV in men or ≥2200 µV in women) and Sokolow‐Lyon criteria (S in V1 + R in V5 or R in V6 ≥ 3500 µV) criteria were used for LVH. To account for the effects of obesity, the BMI‐adjusted Cornell criteria (product of R in aVL + S in V3 and BMI > 60 400 µV kg m−2) and the BMI‐adjusted Sokolow‐Lyon criteria (add 400 µV if overweight, add 800 µV if obese) were used. Compared to traditional ECG‐LVH criteria, more participants met criteria for ECG‐LVH with BMI‐adjusted Cornell voltage (9.9% vs 2.9%) and BMI‐adjusted Sokolow‐Lyon (13.1% vs 6.4%) criteria. In multivariable‐adjusted Cox proportional hazards models, the BMI‐adjusted Sokolow‐Lyon criteria performed no better than traditional criteria (HR 1.18, 95% CI 1.06‐1.32 for all‐cause, HR 1.38, 95% CI 1.17‐1.62 for cardiovascular mortality) and the BMI‐adjusted Cornell voltage criteria attenuated the association with all‐cause (HR 1.16, 95% CI 1.03‐1.32) and cardiovascular mortality (HR 1.34, 95% CI 1.13‐1.60). Despite potential improvements in the detection of LVH using BMI‐adjusted ECG‐LVH criteria, adjusting for BMI may result in the loss of prognostic information.
Gaze deflection reveals how gaze cueing is tuned to extract the mind behind the eyes

Significance We report an empirical study of gaze deflection—a common experience in which you turn to look in a different direction when someone “catches” you staring at them. We show that gaze cueing (the automatic orienting of attention to locations at which others are looking) is far weaker for such displays, even when the actual eye and head movements are identical to more typical intentional gazes. This demonstrates how gaze cueing is driven by the perception of minds, not eyes, and it serves as a case study of both how social dynamics can shape visual attention in a sophisticated manner and how vision science can contribute to our understanding of common social phenomena. Suppose you are surreptitiously looking at someone, and then when they catch you staring at them, you immediately turn away. This is a social phenomenon that almost everyone experiences occasionally. In such experiences—which we will call gaze deflection—the “deflected” gaze is not directed at anything in particular but simply away from the other person. As such, this is a rare instance where we may turn to look in a direction without intending to look there specifically. Here we show that gaze cues are markedly less effective at orienting an observer’s attention when they are seen as deflected in this way—even controlling for low-level visual properties. We conclude that gaze cueing is a sophisticated mental phenomenon: It is not merely driven by perceived eye or head motions but is rather well tuned to extract the “mind” behind the eyes.
Liver cancer prediction in a viral hepatitis cohort: A deep learning approach

Viral hepatitis is the primary cause of liver diseases, among which liver cancer is the leading cause of death from cancer. However, this cancer is often diagnosed in the later stages, which makes treatment difficult or even impossible. This study applied deep learning (DL) models for the early prediction of liver cancer in a hepatitis cohort. In this study, we surveyed 1 million random samples from the National Health Insurance Research Database (NHIRD) to analyze viral hepatitis patients from 2002 to 2010. Then, we used DL models to predict liver cancer cases based on the history of diseases of the hepatitis cohort. Our results revealed the annual prevalence of hepatitis in Taiwan increased from 2002 to 2010, with an average annual percentage change (AAPC) of 5.8% (95% CI: 4.2‐7.4). However, young people (aged 16‐30 years) exhibited a decreasing trend, with an AAPC of −5.6 (95% CI: −8.1 to −2.9). The results of applying DL models showed that the convolution neural network (CNN) model yielded the best performance in terms of predicting liver cancer cases, with an accuracy of 0.980 (AUC: 0.886). In conclusion, this study showed an increasing trend in the annual prevalence of hepatitis, but a decreasing trend in young people from 2002 to 2010 in Taiwan. The CNN model may be applied to predict liver cancer in a hepatitis cohort with high accuracy.
Phosphorus storage in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta) cells and its dependence on phosphate supply

S. Eixler, U. Karsten and U. Selig. 2006. Phosphorus storage in Chlorella vulgaris (Trebouxiophyceae, Chlorophyta) cells and its dependence on phosphate supply. Phycologia 45: 53–60. DOI: 10.2216/04-79.1 It has been postulated that the content of polyphosphates in microalgae can be used as a biological indicator for eutrophication. Therefore the total cellular phosphorus (TCP) and polyphosphate concentration in the green alga Chlorella vulgaris were investigated in relation to the amount of available inorganic phosphorus in the medium. Phosphorus starvation was induced by diluting the cultures with P-free medium to study the impact of this stress situation on phosphorus storage after phosphorus resupply. Increased activity of alkaline phosphatases was used as an indicator for actual phosphorus starvation of algal cells. After a P-starvation phase a significant increase in polyphosphate content could be observed. This effect was detected by using an extraction method and microscopic analysis. The variation in phosphate concentration in the medium of a well-supplied culture had much less effect on the degree of polyphosphate storage than a preceding P-starvation phase. Therefore, the amount of polyphosphate granules in algal cells is not suitable as an indicator for eutrophication (P-loading) of surface waters. The influence of P-starvation on subsequent P-uptake varied between experiments.
Sex Differences in the Inflammatory Consequences of Stress: Implications for Pharmacotherapy

Women are at significantly greater risk of developing stress-related disorders such as depression. The increased risk begins during puberty and continues throughout life until menopause, suggesting a role for ovarian hormones in this increased susceptibility. Importantly, inflammation has been gaining momentum in its role in the pathogenesis of depression. Herein, clinical and preclinical studies have been reviewed to better understand how sex differences within the immune system may contribute to exaggerated risk of depression in females. First, studies that investigate the ability of psychologic stress episodes to engage the inflammatory systems both in the brain and periphery are reviewed with a special focus on sex-specific effects. Moreover, studies are discussed that identify whether imbalanced inflammatory milieu contributes to the development of depression in males versus females and whether these effects are regulated by estradiol. Importantly, we propose a locus coeruleus–norepinephrine–cytokine circuit as a conduit through which stress could increase stress susceptibly in females. Finally, the anti-inflammatory capacity of traditional and nontraditional antidepressants is investigated, with the goal of providing a better understanding of pharmacotherapeutics to enhance strategies to personalize antidepressant treatments between the sexes. The studies reviewed herein strongly support the need for further studies to elucidate whether females are especially sensitive to anti-inflammatory compounds as adjuvants to traditional therapies. SIGNIFICANCE STATEMENT Women have hve an increased risk of developing stress-related disorders such as depression. In this review, literature from clinical and preclinical studies are integrated to define sex differences in stress-induced inflammatory responses as a potential source for the etiology of sex differences in depressive disorders. Moreover, the anti-inflammatory capacity of traditional and nontraditional antidepressants is reviewed to inform on potential pharmacotherapeutic strategies to personalize antidepressant therapy in a sex-dependent manner.