Miracle-dz/UniFAR-FTUs huggingface dataset:
Phyto-ecological analysis of Phytolacca acinosa Roxb. assemblages in Kashmir Himalaya, India

Phyto-ecological studies are essential in understanding community structure, organization and their response to changes in other environmental factors. In this study we analyzed the phytosociological and soil characteristics of Phytolacca acinosa communities and their correlation. 110 quadrats were laid across ten randomly selected sites in Kashmir Himalaya, India. Soil analysis was done using standard protocols. Overall, 161 species were recorded, belonging to 128 genera and 49 families. The species richness (SR) ranges from 27 to 83. Highest IVI was recorded for Poa angustifolia (60.06) and least for Berberis lycium, Abies pindrow, Plectranthus ragosus, and Ailanthus altissima (0.37 each). P. acinosa showed 100% random associations with other plant species. Soil properties varied significantly across the selected sites. Significant positive correlation was found between species richness (SR), Organic matter (OM) (r = 0.79), Organic carbon (OC) (r = 0.79) and Shannon–Wiener index (H) (r = 0.92). Nitrogen content also showed positive correlation with SR and H. Floristic composition of P. acinosa assemblages was governed by soil properties and habitat characteristics of sampling sites. Areas with highest floral diversity had high soil fertility while areas with low soil fertility possess lower diversity and need restoration. The knowledge may prove helpful in management of these habitats, boost conservation and mitigate the effects of changing climate.
Preclinical models of congestive heart failure, advantages, and limitations for application in clinical practice

Congestive heart failure (CHF) has increased over the years, in part because of recent progress in the management of chronic diseases, thus contributing to the maintenance of an increasingly aging population. CHF represents an unresolved health problem and therefore the establishment of animal models that recapitulates the complexity of CHF will become a critical element to be addressed, representing a serious challenge given the complexity of the pathogenesis of CHF itself, which is further compounded by methodological biases that depend on the animal species in use. Animal models of CHF have been developed in many different species, with different surgical procedures, all with promising results but, for the moment, unable to fully recapitulate the human disease. Large animal models often provide a more promising reality, with all the difficulties that their use entails, and which limit their performance to fewer laboratories, the costly of animal housing, animal handling, specialized facilities, skilled methodological training, and reproducibility as another important limiting factor when considering a valid animal model versus potentially better performing alternatives. In this review we will discuss the different animal models of CHF, their advantages and, above all, the limitations of each procedure with respect to effectiveness of results in terms of clinical application.
Quaternions and Cayley Numbers: Algebra and Applications

1 Fundamentals of Linear Algebra. - 1.1 Integers, Rationals and Real Numbers. - 1.2 Real Numbers and Displacements. - 1.3 Groups. - 1.4 Rings and Fields. - 1.5 Linear Spaces. - 1.6 Inner Product Spaces. - 1.7 Algebras. - 1.8 Complex Numbers. - 2 Quaternions. - 2.1 Inventing Quaternions. - 2.2 Quaternion Algebra. - 2.3 The Exponential Form and Root Extraction. - 2.4 Frobenius' Theorem. - 2.5 Inner Product for Quaternions. - 2.6 Quaternions and Rotations in 3- and 4-Dimensions. - 2.7 Relation to the Rotation Matrix. - 2.8 Matrix Formulation of Quaternions. - 2.9 Applications to Spherical Trigonometry. - 2.10 Rotating Axes in Mechanics. - 3 Complexified Quaternions. - 3.1 Scalars, Pseudoscalars, Vectors and Pseudovectors. - 3.2 Complexified Quaternions: Euclidean Metric. - 3.3 Complexified Quaternions: Minkowski Metric. - 3.4 Application of Complexified Quaternions to Space-Time. - 3.5 Quaternions and Electromagnet ism. - 3.6 Quaternionic Representation of Bivectors. - 3.7 Null Tetrad for Space-time. - 3.8 Classification of Complex Bivectors and of the Weyl Tensor. - 4 Cayley Numbers. - 4.1 A Common Notation for Numbers. - 4.2 Cayley Numbers. - 4.3 Angles and Cayley Numbers. - 4.4 Cayley Number Identities. - 4.5 Normed Algebras and the Hurwitz Theorem. - 4.6 Rotations in 7-and 8-Dimensional Euclidean Space. - 4.7 Basis Elements for Cayley Numbers. - 4.8 Geometry of 8-Dimensional Rotations. - Appendix 1 Clifford Algebras. - Appendix 2 Computer Algebra and Cayley Numbers. - References.
Cell-free fetal DNA as a non-invasive method using pyrosequencing in detecting beta-globin gene mutation: A pilot study from area with limited facilities in Indonesia

Background Thalassemia is a monogenic, autosomal recessive, inherited disorder of the red blood cells caused by mutations or deletions in the globin gene. Approximately 6–10% of the Indonesian population carries the β-globin gene mutation; however, premarital screening is rarely conducted, and antenatal screening is optional. We explored the use of cell-free fetal DNA (cffDNA) as a potential non-invasive method of detecting the fetal β-globin gene mutation prenatally in pregnant women. Materials and methods Pregnant mothers (n = 10), who were known carriers of thalassemia and who had a history of having borne a baby with thalassemia major, and their carrier husbands (n = 4) were recruited after providing consent. EDTA blood was drawn, and maternal DNA, including cffDNA, and paternal DNA were isolated. Maternal contamination tests were conducted using the variable number tandem repeat test for ApoB and D1S80 loci. Allele quantification was performed by pyrosequencing. Known mutations from the bio-archived DNA of patients with thalassemia major (n = 16) were run alongside as a control. Results In total, 7 out of 10 cffDNA successfully passed the maternal contamination test. The results of the allele quantification showed that six fetuses were predictive carriers of IVS1nt5 and one was predictive normal, in line with the allele quantification for the bio-archived DNA from patients with thalassemia major. The minimum threshold percentage for mutant A allele at cd26 was 32%, mutant T allele at IVS1nt1 was 23%, and mutant C allele at IVS1nt5 was 39%. Conclusion Taking cffDNA from the mother’s blood proved useful as a non-invasive means of detecting the β-globin gene mutation using pyrosequencing allele quantification. This non-invasive method is of great interest for prenatal diagnosis in settings with limited facilities, as it minimizes the risk of abortion. Further study of other mutations of the β-globin gene is needed.
Multi-epitope chimeric vaccine designing and novel drug targets prioritization against multi-drug resistant Staphylococcus pseudintermedius

Biofilm synthesizing multi-drug resistant Staphylococcus pseudintermedius bacteria has been recognized as the human infectious agent. It has been detected in the diseases of skin, ear, and postoperative infections. Its infections are becoming a major health problem due to its multi-drug resistance capabilities. However, no commercial vaccine for the treatment of its infections is currently available in the market. Here we employed the subtractive proteomics and reverse vaccinology approach to determine the potential novel drug and vaccine targets against S. pseudintermedius infections in humans. After screening the core-proteome of the 39 complete genomes of S. pseudintermedius, 2 metabolic pathways dependent and 34 independent proteins were determined as novel potential drug targets. Two proteins were found and used as potential candidates for designing the chimeric vaccine constructs. Depending on the properties such as antigenicity, toxicity and solubility, multi-epitope based vaccines constructs were designed. For immunogenicity enhancement, different specific sequences like linkers, PADRE sequences and molecular adjuvants were added. Molecular docking and molecular dynamic simulation analyses were performed to evaluate the prioritized vaccine construct’s interactions with human immune cells HLA and TLR4. Finally, the cloning and expression ability of the vaccine construct was determined in the bacterial cloning system and human body immune response was predicted through immune simulation analysis. In conclusion, this study proposed the potential drug and vaccine targets and also designed a chimera vaccine to be tested and validated against infectious S. pseudintermedius species.
Ribosome profiling enhances understanding of mycobacterial translation

A recent addition to the -omics toolkit, ribosome profiling, enables researchers to gain insight into the process and regulation of translation by mapping fragments of mRNA protected from nuclease digestion by ribosome binding. In this review, we discuss how ribosome profiling applied to mycobacteria has led to discoveries about translational regulation. Using case studies, we show that the traditional view of “canonical” translation mechanisms needs expanding to encompass features of mycobacterial translation that are more widespread than previously recognized. We also discuss the limitations of the method and potential future developments that could yield further insight into the fundamental biology of this important human pathogen.
Psychedelic integration: An analysis of the concept and its practice

The concept of integration has garnered increased attention in the past few years, despite a long history of only brief mention. Integration services are offered by therapists, coaches, and other practitioners, or may be self-guided. There are many definitions of psychedelic integration, and the term encompasses a range of practices and techniques. This seems to have led to confusion about what integration is and how it is best practiced. The primary focus of this manuscript is the presentation of the first extensive review and concept analysis of definitions, practices, and models of psychedelic integration. We provide a synthesized definition of integration, synthesized model of integration, and comprehensive summary of integration practices to bring clarity to the subject.
Severe Nuclear Accidents and Learning Effects Severe Nuclear Accidents and Learning Effects

Nuclear accidents with core melting as the ones in Fukushima and Chernobyl play an important role in discussing the risks and chances of nuclear energy. They seem to be more frequent than anticipated. So, we analyse the probability of severe nuclear acci- dents related to power generation. In order to see learning effects of reactor operators, we analyse the number of all known accidents in time. We discuss problems of data acquisition, statistical independence of accidents at the same site and whether the known accidents form a random sample. We analyse core melt accidents with Poisson statistics and derive future accident probabilities. The main part of the chapter is the investigation of the learning effects using generalised linear models with a frequentist and a Bayesian approach and the comparison of the results.
Carcinoembryonic Antigen Interacts with TGF- β Receptor and Inhibits TGF- β Signaling in Colorectal Cancers

As a tumor marker for colorectal cancers, carcinoembryonic antigen (CEA) enhances the metastatic potential of cancer cells. CEA functions as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers. However, the molecular mechanisms by which CEA mediates metastasis remain to be under-stood. Transforming growth factor- β (TGF- β ) signaling regulates both tumor suppression and metastasis, and also contributes to the stimulation of CEA transcription and secretion in colorectal cancer cells. However, it remains unknown whether CEA, in turn, influences TGF- β functions and if a regulatory cross-talk exists between CEA and the TGF- β signaling pathway. Here, we report that CEA directly interacts with TGF- β receptor and inhibits TGF- β signaling. Targeting CEA with either CEA-specific antibody or siRNA rescues TGF- β response in colorectal cancer cell lines with elevated CEA, thereby restoring the inhibitory effects of TGF- β signaling on proliferation. CEA also enhances the survival of colorectal cancer cells in both local colonization and liver metastasis in animal study. Our study provides novel insights into the interaction between CEA and TGF- β signaling pathway and establishes a negative feedback loop in amplifying the progression of colon cancer cells to more invasive phenotypes. These findings offer new therapeutic opportunities to inhibit colorectal cancer cell proliferation by cotargeting CEA in promoting tumor-inhibitory action of the TGF- β pathway. Cancer Res; 70(20); 8159 ©2010 TBRI with Smad3 was attenuated in the presence of CEA compared with in the absence of CEA. We then sought to determine if Smad3 phosphorylation was modified by CEA. 293T cells were transfected with or without CEA for 24 hours, and then stimulated with TGF- β for different time periods. Cells were harvested and the levels of p-Smad3 and Smad3 proteins were evaluated by Western blotting.
Mixing of a Turbulent Wall-Jet into a Free-Stream

Turbulent diffusion of 2-dimensional, plane wall-jet into moving, uniform free-stream with pressure gradient of zero is investigated; in order to check similarity conditions far downstream, investigation was conducted to include 465 slot widths downstream of slot; method is given for reducing results of this investigation to those of previous investigators who studied mixing of wall-jet in stationary free-stream.
Optical frequency transfer over submarine ﬁber links: supplementary material

This document provides supplementary information to "Optical frequency transfer over submarine fiber links," https://doi.org/10.1364/OPTICA.5. 000893. Here we provide additional details on the realization of the experiment. We describe the ultra-stable laser source used for the experiment, describe the interaction of optical and electromagnetic field s in a high-voltage cable, and derive the phase noise of a two-way fiber-based clock s comparison where a fiber pair is used instead of a single fiber.
Regulation of Endothelin-1 Expression in the Bovine Corpus Luteum: Elevation by Prostaglandin FSa

Prostaglandin F,, (PGF,,) has been recognized as the physiolog- ical luteolysin in ruminants and other species for more than three decades; however, the mechanisms involved in its action are poorly understood. We previously have shown that endothelin-1 (ET-l) me- diates, at least in part, the action of PGF,,, and the current study examines the effect of PGF,, on the expression of ET-l in bovine corpus luteum (CL). Endothelins (ETs) were extracted from CL, col- lected at various times of the estrous cycle, and highest levels were found during luteolysis. The expression of prepro-ET-l was also high- est in regressing CL, suggesting that PGF,, may have elevated ET-l expression. This was confirmed by demonstrating that administra- tion of PGF,,
Sequence Proﬁle-based detection of microRNA precursors in animal genomes

Motivation: MicroRNAs (miRNA) are essential 21–22 nt regulatory RNAs produced from larger hairpin-like precursors. Local sequence alignment tools such as BLAST are able to identify new members of known miRNA families, but not all of them. We set out to estimate how many new miRNAs could be recovered using a proﬁle-based strategy such as that implemented in the ERPIN program. Results: We constructed alignments for 18 miRNA families and performed ERPIN searches on animal genomes. Results were compared to those of a WU-BLAST search at the same E -value cutoff. The two combined approaches produced 265 new miRNA candidates that were not found in miRNA databases. About 17% of hits were ERPIN speciﬁc. They showed better structural characteristics than BLAST-speciﬁc hits and included interesting candidates such as members of the miR-17 cluster in Tetraodon . Proﬁle-based RNA detection will be an important complement of similarity search programs in the completion of miRNA collections.
The Information Technologies Outcome on Total Quality Management as the Most Important Study

— Both of Total Quality Management (TQM) and Information Technology (IT) have considerably impacted on most organizations and each one has been generally researched. on the other hand, there is little well-founded practical research on the relationship between the two, particularly on the way in which TQM is influenced by IT. There is little doubt that applications of IT influence all parts and functions of a company, therefore, it is argued that IT also must affect Total Quality Management (TQM). This paper propose a study of such relationships through an analysis of industrial companies based in Libya. The data will be got will show that the most concentrated users of IT identify a bigger impact on their TQM scope.
Iterative blind deconvolution method using Lucy's algorithm

Lucy's algorithm is iteratively applied in a blind deconvolution method. This new approach permits the reconstruction of a greatly extended object from one speckle frame. Speckle images of extended object may lie partly outside the active region of the detector. Likewise, objects close to the boundary of the detector, but outside of the field of view, may contaminate the image. The blind deconvolution method is adapted to deal with these situations. Repeated use of the blind deconvolution method is also proposed. Computer simulations are conducted and the effectiveness of the proposed method is demonstrated. The method is applied to an image of the solar photosphere
Virus-Like Particles Presenting the FGF-2 Protein or Identi ﬁ ed Antigenic Peptides Promoted Antitumor Immune Responses in Mice

Background: Fibroblast growth factor (FGF)-2 is overexpressed in various tumor tissues. It affects tumor cell proliferation, invasion and survival, promotes tumor angiogenesis and is tightly involved in the development of systemic and local immunosuppressive tumor mechanisms. Purpose: This study aimed to develop an effective vaccine against FGF-2 and to investigate the effects of anti-FGF-2 immunization on tumor growth and antitumor immune responses. Methods: A set of thirteen synthesized overlapping peptides covering all possible linear B-cell epitopes of murine FGF-2 and a recombinant FGF-2 protein were conjugated to virus-like particles (VLPs) of recombinant hepatitis B core antigen (HBcAg). The VLPs were immunized through a preventive or therapeutic strategy in a TC-1 or 4T1 grafted tumor model. Results: Immunization with FGF-2 peptides or full-length protein-coupled VLPs produced FGF-2-speci ﬁ c antibodies with a high titer. Peptide 12, which is located in the heparin-binding site of FGF-2, or protein-conjugated VLPs presented the most signi ﬁ cant effects on the suppression of TC-1 tumor growth. The levels of IFN- γ -expressing splenocytes and serum IFN- γ were signi ﬁ cantly elevated; further, the immune effector cells CD8 + IFN- γ + cytotoxic T lymphocytes (CTLs) and CD4 + IFN- γ + Th1 cells were signi ﬁ cantly increased, whereas the immunosuppressive cells CD4 + CD25 + FOXP3 + Treg cells and Gr-1 + CD11b + myeloid-derived suppressor cells (MDSCs) were decreased in the immunized mice. In addition, VLP immunization signi ﬁ cantly suppressed tumor vascularization and promoted tumor cell apoptosis. In mice bearing 4T1 breast tumor, preventive immunization with FGF-2-conjugated VLPs suppressed tumor growth and lung metastasis, and increased effector cell responses. Conclusion: Active immunization against FGF-2 is a new possible strategy for tumor immunotherapy.
Effects of H-NS and Potassium Glutamate on (cid:115) S - and (cid:115) 70 -Directed Transcription In Vitro from Osmotically Regulated P1 and P2 Promoters of proU in Escherichia coli

We have used supercoiled DNA templates in this study to demonstrate that transcription in vitro from the P1 and P2 promoters of the osmoresponsive proU operon of Escherichia coli is preferentially mediated by the (cid:115) S - and (cid:115) 70 -bearing RNA polymerase holoenzymes, respectively. Addition of potassium glutamate resulted in the activation of transcription from both P1 and P2 and also led to a pronounced enhancement of (cid:115) S selectivity at the P1 promoter. Transcription from P2, and to a lesser extent from P1, was inhibited by the nucleoid protein H-NS but only in the absence of potassium glutamate. This study validates the existence of dual promoters with dual speciﬁcities for proU transcription. Our results also support the proposals that potassium, which is known to accumulate in cells grown at high osmolarity, is at least partially responsible for effecting the in vivo induction of proU transcription and that it does so through two mechanisms, directly by the activation of RNA polymerase and indirectly by the relief of repression imposed by H-NS. The
SURVEY OF AMERICAN AND EUROPEAN FOULBROOD DISEASE ON HONEY BEE COLONIES IN DAKAHLIA GOVERNORATE.

It is essential that beekeepers are able to recognize the diseses and distinguish them from foul brood. Survey was carried out in four districts of Dakahlia governorate (Mansura, Metghamer, AAll--MMeett ssaallssiill and Bilqas) from Sept 2009 to Aug2011. Two bacterial diseases of the honey bee larvae were widespread in all districts which were American and European foulbrood diseases (AFB and EFB). AFB disease is one of the most destructive diseases of the honey bee in all apiaries during autumn and winter however; it is spread sometimes during spring. EFB disease is a bacterial disease that effects honey bee larvae before the capped stage, it spread in all districts during spring and summer.