Miracle-dz/UniFAR-FTUs huggingface dataset:
The Role of Exosome and the ESCRT Pathway on Enveloped Virus Infection

The endosomal sorting complex required for transport (ESCRT) system consists of peripheral membrane protein complexes ESCRT-0, -I, -II, -III VPS4-VTA1, and ALIX homodimer. This system plays an important role in the degradation of non-essential or dangerous plasma membrane proteins, the biogenesis of lysosomes and yeast vacuoles, the budding of most enveloped viruses, and promoting membrane shedding of cytokinesis. Recent results show that exosomes and the ESCRT pathway play important roles in virus infection. This review mainly focuses on the roles of exosomes and the ESCRT pathway in virus assembly, budding, and infection of enveloped viruses. The elaboration of the mechanism of exosomes and the ESCRT pathway in some enveloped viruses provides important implications for the further study of the infection mechanism of other enveloped viruses.
Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway

Low-magnitude high-frequency vibration (LMHFV) has previously been reported to modulate the acute inflammatory response of ovariectomy-induced osteoporotic fracture healing. However, the underlying mechanisms are not clear. In the present study, we investigated the effect of LMHFV on the inflammatory response and the role of the p38 MAPK mechanical signaling pathway in macrophages during the healing process. A closed femoral fracture SD rat model was used. In vivo results showed that LMHFV enhanced activation of the p38 MAPK pathway at the fracture site. The acute inflammatory response, expression of inflammatory cytokines, and callus formation were suppressed in vivo by p38 MAPK inhibition. However, LMHFV did not show direct in vitro enhancement effects on the polarization of RAW264.7 macrophage from the M1 to M2 phenotype, but instead promoted macrophage enlargement and transformation to dendritic monocytes. The present study demonstrated that p38 MAPK modulated the enhancement effects of mechanical stimulation in vivo only. LMHFV may not have exerted its enhancement effects directly on macrophage, but the exact mechanism may have taken a different pathway that requires further investigation in the various subsets of immune cells.
A Multi-Band Near Perfect Polarization and Angular Insensitive Metamaterial Absorber With a Simple Octagonal Resonator for Visible Wavelength

A multi-band, ultrathin, polarization-insensitive, near-perfect metamaterial absorber (PMA) has been proposed and substantiated numerically for solar thermophotovoltaic (STPV) systems which also can be used in some other applications. This kind of absorber currently drawing massive interest throughout the research of optics. Especially in solar harvesting metamaterial absorbers can give a huge boost in efficiency by intensifying the solar electromagnetic wave. Visible wavelength has been the key focus of the proposed design so that the structure can utilize solar energy proficiently. Aluminum (Al) and Gallium Arsenide (GaAs) have been chosen as materials for their higher electron mobility along with good temperature stability. The PMA is a three-layer metal-dielectric-metal called sandwiched structure. For proper characterization of the PMA absorber, extensive parametric inspections were carried out with underlying physics. The finite integration technique (FIT) in computer simulation technology microwave studio (CST MWS) is used to perform the numerical analysis and for verification finite element method (FEM) in COMSOL Multiphysics has been used along with interference theory model (ITM) for calculating the absorbance. The PMA shows 99.27%, 99.89%, 99.91%, and 99.06% perfect absorption at 454.75nm, 505.53nm, 568.72nm, and 600.85nm resonance wavelength in all three modes of waveguide propagation. The design also exhibits incident wave stability up to 60° for both transverse electric (TE), and transverse magnetic (TM) wave modes. Excellent glucose concentration sensing ability was also observed with the proposed structure. So, the proposed PMA can be implemented in solar energy harvesting devices along with solar sensors or detectors, light trappers, light modulators, or light wavelength detectors.
ZC3HC1 Is a Novel Inherent Component of the Nuclear Basket, Resident in a State of Reciprocal Dependence with TPR

The nuclear basket (NB) scaffold, a fibrillar structure anchored to the nuclear pore complex (NPC), is regarded as constructed of polypeptides of the coiled-coil dominated protein TPR to which other proteins can bind without contributing to the NB’s structural integrity. Here we report vertebrate protein ZC3HC1 as a novel inherent constituent of the NB, common at the nuclear envelopes (NE) of proliferating and non-dividing, terminally differentiated cells of different morphogenetic origin. Formerly described as a protein of other functions, we instead present the NB component ZC3HC1 as a protein required for enabling distinct amounts of TPR to occur NB-appended, with such ZC3HC1-dependency applying to about half the total amount of TPR at the NEs of different somatic cell types. Furthermore, pointing to an NB structure more complex than previously anticipated, we discuss how ZC3HC1 and the ZC3HC1-dependent TPR polypeptides could enlarge the NB’s functional repertoire.
HERV-K Modulates the Immune Response in ALS Patients

Human endogenous retrovirus (HERV)-K env-su glycoprotein has been documented in amyotrophic lateral sclerosis (ALS), where HERV-K env-su 19–37 antibody levels significantly correlated with clinical measures of disease severity. Herein, we investigated further the humoral and cell-mediated immune response against specific antigenic peptides derived from HERV-K in ALS. HERV-K env glycoprotein expression on peripheral blood mononuclear cells (PBMCs) membrane and cytokines and chemokines after stimulation with HERV-K env 19–37 and HERV-K env 109–126 were quantified in patients and healthy controls (HCs). HERV-K env glycoprotein was more expressed in B cells and NK cells of ALS patients compared to HCs, whereas HERV-K env transcripts were similar in ALS and HCs. In ALS patients, specific stimulation with HERV-K env 109–126 peptide showed a higher expression of IL-6 by CD19/B cells. Both peptides, however, were able to induce a great production of IFN-γ by stimulation CD19/B cells, and yielded a higher expression of MIP-1α and a lower expression of MCP-1. HERV-K env 19–37 peptide induced a great production of TNF-α in CD8/T cells. In conclusion, we observed the ability of HERV-K to modulate the immune system, generating mediators mainly involved in proinflammatory response.
The Vector Control Scheme for Amphibious Spherical Robots Based on Reinforcement Learning

Due to variable underwater working conditions and unfavorable environments, it is difficult to design a controller suitable for underwater robots. This paper uses the adaptive ability of reinforcement learning to propose a two-layer network framework based on reinforcement learning to realize the control of amphibious spherical robots. The upper planning layer mainly plans the total torque of the robot at each moment according to the desired position and speed. The lower control layer mainly configures the parameters of the four machine legs according to the planning instructions of the upper planning layer. Through the cooperation of the planning layer and the control layer, the adaptive motion control of the amphibious spherical robot can finally be realized. Finally, the proposed scheme was verified on a simulated amphibious spherical robot.
What Happens When People with Depression Gather Online?

Depression is a common mental disease that impacts people of all ages and backgrounds. To meet needs that cannot otherwise be met, people with depression or who tend to suffer from depression often gather in online depression communities. However, since joining a depression community exposes members to the depression of others, the impact of such communities is not entirely clear. This study therefore explored what happens when people with depression gather in Sina Weibo’s Depression Super Topic online community. Through website crawling, postings from Depression Super Topic were compared with postings from members’ regular timelines with respect to themes, emotions disclosed, activity patterns, and the number of likes and comments. Topics of distilled postings covering support, regulations, emotions and life sharing, and initiating discussions were then coded. From comparison analysis, it was found that postings in the Depression Super Topic community received more comments and disclosed more emotions than regular timelines and that members were more active in the community at night. This study offers a picture of what occurs when people with depression gather online, which helps better understand their issues and therefore provide more targeted support.
Regulation of CTLA-4 and PD-L1 Expression in Relapsing-Remitting Multiple Sclerosis Patients after Treatment with Fingolimod, IFNβ-1α, Glatiramer Acetate, and Dimethyl Fumarate Drugs

Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system (CNS) that is characterized by inflammation which typically results in significant impairment in most patients. Immune checkpoints act as co-stimulatory and co-inhibitory molecules and play a fundamental role in keeping the equilibrium of the immune system. Cytotoxic T-lymphocyte antigen-4 (CTLA-4) and Programmed death-ligand 1 (PD-L1), as inhibitory immune checkpoints, participate in terminating the development of numerous autoimmune diseases, including MS. We assessed the CTLA-4 and PD-L1 gene expression in the different cell types of peripheral blood mononuclear cells of MS patients using single-cell RNA-seq data. Additionally, this study outlines how CTLA-4 and PD-L1 expression was altered in the PBMC samples of relapsing-remitting multiple sclerosis (RRMS) patients compared to the healthy group. Finally, it investigates the impact of various MS-related treatments in the CTLA-4 and PD-L1 expression to restrain autoreactive T cells and stop the development of MS autoimmunity.
Insecticidal Activity of Lemongrass Essential Oil as an Eco-Friendly Agent against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae)

Simple Summary The insect pest, black cutworm, Agrotis ipsilon (Lepidoptera: Noctuidae), attacks the seedling stage of many field crops in several countries around the world. To control this insect pest, in this study, lemongrass (Cymbopogon citratus) essential oil observed potential insecticidal activity against the second-instar larvae of A. ipsilon. Abstract Background: The destructive insect pest Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) is a polyphagous species targeting many economically important plants. The extensive and arbitrary use of insecticides has resulted in the build-up of insecticide resistance and pesticide residues accumulating in food. Therefore, it is becoming evident that alternative pest management tools are needed to reduce risks to humans, the environment, and non-target organisms, and at the same time, they should be used in field application at the lowest cost. Methods: In view of this objective, the present study demonstrates the toxicity of lemongrass (Cymbopogon citratus (DC.) Stapf) essential oil (EO), against the black cutworm A. ipsilon under controlled laboratory conditions in terms of measuring the activity of peroxidase and detoxification enzymes. The chemical components of the EO were analyzed using GC–MS. Results: The results show that after 96 h post treatment, the LC15 and LC50 values were 427.67 and 2623.06 mg/L, respectively, of C. citratus EO on second-instar larvae of A. ipsilon. A slight significance in elongation of the larval duration with LC15 and LC50 value was found with control. By GC–MS analysis, the main compounds identified in the EO were α-citral and β-citral with percentages of 35.91%, and 35%, respectively. The oxidative stress indicates a significant increase in CAT and lipid peroxidase enzyme activity after 96 h post treatment at the LC15 and LC50. Conversely, the detoxification enzyme activity shows an inhibition of CarE and GST enzymes of larvae exposed to LC15 and LC50 values in response to C. citratus EO. Conclusions: The present data show that lemongrass EO has insecticidal activity against the black cutworm, A. ipsilon.
Simulated dose painting of hypoxic sub-volumes in pancreatic cancer stereotactic body radiotherapy

Dose painting of hypoxic tumour sub-volumes using positron-emission tomography (PET) has been shown to improve tumour control in silico in several sites, predominantly head and neck and lung cancers. Pancreatic cancer presents a more stringent challenge, given its proximity to critical gastro-intestinal organs-at-risk (OARs), anatomic motion, and impediments to reliable PET hypoxia quantification. A radiobiological model was developed to estimate clonogen survival fraction (SF), using 18F-fluoroazomycin arabinoside PET (FAZA PET) images from ten patients with unresectable pancreatic ductal adenocarcinoma to quantify oxygen enhancement effects. For each patient, four simulated five-fraction stereotactic body radiotherapy (SBRT) plans were generated: (1) a standard SBRT plan aiming to cover the planning target volume with 40 Gy, (2) dose painting plans delivering escalated doses to a maximum of three FAZA-avid hypoxic sub-volumes, (3) dose painting plans with simulated spacer separating the duodenum and pancreatic head, and (4), plans with integrated boosts to geometric contractions of the gross tumour volume (GTV). All plans saturated at least one OAR dose limit. SF was calculated for each plan and sensitivity of SF to simulated hypoxia quantification errors was evaluated. Dose painting resulted in a 55% reduction in SF as compared to standard SBRT; 78% with spacer. Integrated boosts to hypoxia-blind geometric contractions resulted in a 41% reduction in SF. The reduction in SF for dose-painting plans persisted for all hypoxia quantification parameters studied, including registration and rigid motion errors that resulted in shifts and rotations of the GTV and hypoxic sub-volumes by as much as 1 cm and 10 degrees. Although proximity to OARs ultimately limited dose escalation, with estimated SFs (∼10−5) well above levels required to completely ablate a ∼10 cm3 tumour, dose painting robustly reduced clonogen survival when accounting for expected treatment and imaging uncertainties and thus, may improve local response and associated morbidity.
Anisotropic Strange Star in 5D Einstein-Gauss-Bonnet Gravity

In this paper, we investigated a new anisotropic solution for the strange star model in the context of 5D Einstein-Gauss-Bonnet (EGB) gravity. For this purpose, we used a linear equation of state (EOS), in particular pr=βρ+γ, (where β and γ are constants) together with a well-behaved ansatz for gravitational potential, corresponding to a radial component of spacetime. In this way, we found the other gravitational potential as well as main thermodynamical variables, such as pressures (both radial and tangential) with energy density. The constant parameters of the anisotropic solution were obtained by matching a well-known Boulware-Deser solution at the boundary. The physical viability of the strange star model was also tested in order to describe the realistic models. Moreover, we studied the hydrostatic equilibrium of the stellar system by using a modified TOV equation and the dynamical stability through the critical value of the radial adiabatic index. The mass-radius relationship was also established for determining the compactness and surface redshift of the model, which increases with the Gauss-Bonnet coupling constant α but does not cross the Buchdahal limit.
Impact of Dietary Lipids on the Reverse Cholesterol Transport: What We Learned from Animal Studies

Reverse cholesterol transport (RCT) is a physiological mechanism protecting cells from an excessive accumulation of cholesterol. When this process begins in vascular macrophages, it acquires antiatherogenic properties, as has been widely demonstrated in animal models. Dietary lipids, despite representing a fundamental source of energy and exerting multiple biological functions, may induce detrimental effects on cardiovascular health. In the present review we summarize the current knowledge on the mechanisms of action of the most relevant classes of dietary lipids, such as fatty acids, sterols and liposoluble vitamins, with effects on different steps of RCT. We also provide a critical analysis of data obtained from experimental models which can serve as a valuable tool to clarify the effects of dietary lipids on cardiovascular disease.
Quantitative Tyrosine Phosphoproteome Profiling of AXL Receptor Tyrosine Kinase Signaling Network

Simple Summary AXL is a receptor tyrosine kinase belonging to the TAM (Tyro3, Axl and Mer) family. The AXL protein plays an important role in promoting cancer development, such as proliferation, migration, invasion and survival of cancer cells. In this study, we used mass spectrometry-based proteomics to quantify the cancer signaling regulated by AXL activation. Our study identified more than 1000 phosphotyrosine sites and discovered that activation of AXL can upregulate multiple cancer-promoting and cell migration/invasion-related signaling pathways. We also observed significant crosstalk as evidenced by rapid phosphorylation of multiple receptor tyrosine kinases and protein tyrosine phosphatases, including PTPN11 and PTPRA, upon GAS6 stimulation. These discoveries should serve as a potentially useful resource for studying AXL functions as well as for the development of effective therapeutic options to target AXL. Abstract Overexpression and amplification of AXL receptor tyrosine kinase (RTK) has been found in several hematologic and solid malignancies. Activation of AXL can enhance tumor-promoting processes such as cancer cell proliferation, migration, invasion and survival. Despite the important role of AXL in cancer development, a deep and quantitative mapping of its temporal dynamic signaling transduction has not yet been reported. Here, we used a TMT labeling-based quantitative proteomics approach to characterize the temporal dynamics of the phosphotyrosine proteome induced by AXL activation. We identified >1100 phosphotyrosine sites and observed a widespread upregulation of tyrosine phosphorylation induced by GAS6 stimulation. We also detected several tyrosine sites whose phosphorylation levels were reduced upon AXL activation. Gene set enrichment-based pathway analysis indicated the activation of several cancer-promoting and cell migration/invasion-related signaling pathways, including RAS, EGFR, focal adhesion, VEGFR and cytoskeletal rearrangement pathways. We also observed a rapid induction of phosphorylation of protein tyrosine phosphatases, including PTPN11 and PTPRA, upon GAS6 stimulation. The novel molecules downstream of AXL identified in this study along with the detailed global quantitative map elucidating the temporal dynamics of AXL activation should not only help understand the oncogenic role of AXL, but also aid in developing therapeutic options to effectively target AXL.
Data Center Aggregators’ Optimal Bidding and Benefit Allocation Strategy Considering the Spatiotemporal Transfer Characteristics

The aggregator, an emerging entity in the electricity market, gathers and creates market power for the small flexible resources, which traditionally contain distributed generation, electric storage, and flexible load. Recently, the exploding growth of information technology demand gives rise to the data centers, whose participation in demand response (DR) is becoming increasingly significant for its remarkable performance in spatiotemporal transfer characteristics compared to conventional facilities. The high-quality DR potential of data centers provides a new opportunity for the profit-seeking data center aggregators (DCAs) to optimize their bidding plans in the day-ahead electricity market. Therefore, this article targets the formulation of the optimal bidding strategy of demand response aggregators (DRAs) to achieve the maximization of the joint benefit of both DCA and data centers. A spatiotemporal transfer characteristics-based optimization strategy is formulated to obtain the electricity purchasing scheme and arrangement of data centers. Meanwhile, the Shapley value method is introduced to design the appropriate and equitable DR reward mechanism that each DCA should compensate its customers, which would serve as a guarantee to ensure data centers’ active participation in DR events and lay a solid foundation for the implementation of the DCA's optimal bidding strategy. Numerical tested results demonstrate the effectiveness of the proposed optimal bidding model and the benefit allocation mechanism, which profit both DRA and data centers when participating in DR programs while preserving their daily power demand.
Evaluation of β-Catenin Inhibition of Axitinib and Nitazoxanide in Human Monocyte-Derived Dendritic Cells

Modulation of β-catenin signaling has attractive therapeutic potential in cancer immunotherapy. Several studies have found that β-catenin can mediate immune evasion in cancer and promote anti-inflammatory features of antigen-presenting dendritic cells. Many small molecular compounds that inhibit Wnt/β-catenin signaling are currently in clinical development, but none have entered routine clinical use. New inhibitors of β-catenin signaling are consequently desirable. Here, we have tested, in monocyte-derived dendritic cells, the effects of two small molecular compounds, axitinib and nitazoxanide, that previously have been discovered to inhibit β-catenin signaling in colon cancer cells. Immature and lipopolysaccharide-matured dendritic cells prepared from healthy blood donor buffy coats were stimulated with 6-bromoindirubin-3′-oxime (6-BIO) to boost basal β-catenin activity, and the effects of axitinib and nitazoxanide were compared with the commercial β-catenin inhibitor ICG-001. Assays, including genome-wide RNA-sequencing, indicated that neither axitinib nor nitazoxanide demonstrated considerable β-catenin inhibition. Both compounds were found to be less toxic to monocyte-derived dendritic cells than either 6-BIO or ICG-001. Axitinib stimulated several aspects of dendritic cell function, such as IL12-p70 secretion, and counteracted IL-10 secretion, according to the present study. However, neither axitinib nor nitazoxanide were found to be efficient β-catenin inhibitors in monocyte-derived dendritic cells.
Red Emission Carbon Dots Prepared by 1,4-Diaminonaphthalene for Light-Emitting Diode Application and Metal Ion Detection

Carbon dots (CDs), as the most important type of carbon materials, have been widely used in many fields because of their unique fluorescence characteristics and excellent properties of biocompatibility. In previous studies, the fluorescence of CDs was mainly concentrated in the blue and green, whereas the red fluorescence was relatively less. Herein, we prepared efficient red-emitting CDs from 1,4-diaminonaphthalene using solvothermal methods. We discussed the effects of different solvothermal solvents on CDs. The results show that CDs prepared with octane and acetone as reaction media have the best fluorescence properties. The CDs dispersed in different organic solvents exhibited tunable emission across a wide spectrum from 427 nm to 679 nm. We further demonstrated the application of red light-emitting diode (LED) optoelectronics and fluorescence detection of Fe3+ in aqueous solution.