Miracle-dz/UniFAR-FTUs huggingface dataset:
MORPHOLOGICAL, MOLECULAR AND PATHOGENICITY CHARACTERIZATION OF PYTHIUM SPP. ON ZUCCHINI ISOLATED FROM SOIL AND DISEASED PLANTS IN IRAQ

The study was conducted at Plant Protection Dept., College of Agricultural Engineering Sciences / University of Baghdad during 2018-2019. Twenty four isolates of Pythium spp. were isolate from different regions from diseased seedling and soil samples. Fourteen isolates recovered from seven regions in Baghdad and ten isolates from three regions in Al-Anbar. Pythium spp. were widespread in most of the cucurbits fields in the two provinces. Four media type (V8A, PDA, CMA and CA) were capable of growing. The highest growth rate was on V8-A and the lowest growth rate was on CA media. All isolates were producing asexual and sexual structures. Sporangia were produce on CMA media within seven days at 25°C. Oospores were produce on V8-A and CA media after 14 day. The optimum temperature for all isolates growth was 30 and 25°C. The results of morphological characteristics show that S2 and S3 isolates were belong to Pythium deliense and this was the first report in Iraq. The other isolates were identified as P. aphanidermatum. Identification was confirmed by molecular characterization of isolates based on ITS region using ITS1&ITS4 primers. All 24 isolates sequences were deposited in the NCBI GeneBank database under the accession numbers (MN365079 MN365100) and (MN365124 & MN365125). The phylogenetic tree of the 24 isolates in this study and other reference species from GeneBank were designed based on the distance method (neighbor-joining) using MEGAX program. Variations among the isolates were found. Also Iraqi isolates were differed from GeneBank isolates. Pathogenicity test for 24 isolate show that the two species were pathogenic on Zucchini and all the isolates were differed in pathogenicity causing pre and post emergence damping – off. Pre emergence was 10 – 52% and post emergence was 11 – 41%. The pathogenicity of P. deliense and P. aphanidermatum isolates were confirm for the first time on Zucchini.
A new species of rainbowfish (Melanotaeniidae: Melanotaenia), from Batanta Island, western New Guinea

A new species of rainbowfish, Melanotaenia synergos, is described on the basis of 45 specimens, 24.1-63.3 mm SL, collected in 1998 at Batanta Island, western New Guinea (Papua Barat Province, Indonesia). It is closely allied to M. catherinae from the nearby island of Waigeo in the Raja Ampat Group. The two species share similar meristic and morphological features as well as general colour pattern sim- ilarities. However, they differ in modal counts for pectoral- fin rays and lateral scales. They also exhibit slight colour pat- tern differences related to the width of the dark midlateral stripe, which is generally narrower in M. synergos, covering one and a half scale rows for most of its length versus 2 to 3 scale rows for M. catherinae. Analysis of genetic relationships based on cytochrome b sequences indicates a close relation- ship between the two species. Of four species that were analysed (M. synergos, M. catherinae, M. batanta, and M. affinis) the mean Kimura 2-parameter genetic divergences between species varied from 1.3 to 17.1%. The new species differed from M. catherinae by between 2.4 and 2.7%. Zusammenfassung Eine neue Art der Regenbogenfische: Melanotaenia synergos,
Evaluation of Tensile Bond Strength of Heat‑Polymerized Acrylic Soft Liners with Various Surface Pre‑Treatment of Denture Bases: An In Vitro Study

Background: Resilient liners are prone to bond failure with acrylic resin base, creating an environment for bacterial growth and accelerated break down of soft liner, resulting in a deteriorating prosthesis. Aims and Objectives: The purpose of the present study is to evaluate the efficacy of mechanical, chemical, and mechanochemical pre-treatment of denture base resin on the tensile bond strength of heat-cured permanent resilient liners. Materials and Methods: A total of 320 cuboidal blocks (40 mm ×x 10 mm x 10 mm) of heat-polymerized acrylic resin (Trevalon) were made which were equally grouped into Permasoft (Group 1) and Acrasoft (Group 2). Each group was further subdivided into four subgroups based on surface pre-treatment of denture base, namely: Control, chemical (methyl methacrylate [MMA]), mechanical (sandblasting with 250 μ aluminum oxide particles), and mechanochemical (sandblasting followed by MMA). A 3-mm thick resilient denture liner was packed between two resin blocks. Then, each subgroup was divided into two test groups (n = 10) for testing tensile bond strength (using universal testing machine) after 24 h and 30 days post-immersion in artificial saliva. One-way ANOVA and Tukey test were used for data analysis. Results: The tests resulted in greater tensile bond strength for Acrasoft group compared to Permasoft at both 24 h and 1 month. Between the two time periods, it was more for 24 h group. Among all methods, mechanical method resulted in superior tensile bond strength. Conclusion: This in vitro study concluded that all the tested pre-treatment methods showed better tensile bond strength than control group, maximum with mechanical group.
Analysis of Bacterial Communities in Aerobic Solid-Fermentation Culture of Zhenjiang Hengshun Vinegar

The constitution of the bacterial community in Zhenjiang Hengshun vinegar acetic fermentation was analyzed by culture-independent approach. Total DNA was extracted and amplified using PCR reaction to construct 16S rDNA library, which includes 96 positive clones. Through sequencing of those clones, the phylogenetic tree is illustrated by representative sequences. Based on phylogenetic analyzing, 16 OUTs was obtained, and the composition of microorganisms in Hengshun vinegar fementation was concluded as below: five OUTs belong to genus Lactobacillus, two are genus Acetobacter, one genus Gluconacetobacter, two genus Staphylococcus, two genus Enterobacter, two genus Pseudomonas, one genus Flavobacterium, and one genus Sinorhizobium, respectively.
Assessing face image quality with LSTMs

Biometric authentication using ngerprints or face recognition is making its way intothe mainstream, and there is an urgent need to make these authentication methods assecure and reliable as possible. One way to achieve better performance with a biometricauthentication method, is to introduce a quality estimation step early in the pipeline, sothat unsuitable, or low-quality samples can be rejected.While existing work predominantly focuses on algorithms for detecting specic propertiesof the face images, we investigate whether machine learning techniques can provide ageneral way to estimate overall face image quality.We train a selection of neural network types, and discover that a type of RecurrentNeural Network (RNN) called Long Short-Term Memory (LSTM) can reliably estimateface image quality, with better performance than the bespoke algorithms.
A new sclerite-free genus and species of Clavulariidae (Coelenterata: Octocorallia)

This short communication describing only a single new taxon is necessary to facilitate further research publications by Neil Blackstone & Austin Parrin (Northern Illinois University) and colleagues. Neil and Austin have found the new species to be an ideal experimental animal that is fast-growing and extremely easy to maintain in laboratory aquaria (Parrin et al. 2010). [NTM = Museum & Art Gallery of the Northern Territory, PO Box 4646, Darwin, NT 0801, Australia].
Multiple Shoot Regeneration from Nodal Explants of Gynura procumbens (Lour.) Merr

Gynura procumbens (Lour.) Merr. is an important medicinal plants and used to treat various ailments such as diabetes, hypertension and urinary tract infection. Multiple shoot formation was induced from nodal segment of G. procumbens cultured on Murashige and Skoog (MS) medium containing various concentrations 6-benzylaminopurine (BAP). The MS medium with 3 mg/l BAP gave the highest number of shoots per explant. All the micro-shoots produced normal roots within two weeks of culture on basic MS medium without any plant growth regulators. Regenerated plants were aclimatised before transferred to field condition and showed similar morphology to field grown plants.
NAD(P)H:quinone oxidoreductase gene polymorphism and susceptibility of lung cancer

Objectives:To investigate whether lung cancer is associated with genetic polymorphism of NQO1 gene (NAD(P) H:quinone oxidoreductase gene)caused by the point mutation of cDNA 609 C to T Methods:The polymorphism of NQO1 gene was analyzed in 95 patients with lung cancer and 136 unrelated healthy adult individuals by utilizing polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) assay Results:In this study, the frequencies of T allele were 54%and 43%in the lung cancer group and the health control group respectively, which was significantly different between the two groups(P=0 024) The allelic frequency of the mutant T allele was significantly higher in the lung cancer patients as compared to controls There was a significant difference in the overall genotypical distribution between the patients and the controls (P=0 008) The risk of suffering from lung cancer was increased 2 949 times in the individuals with T/C genotype (95%CI:1 426～6 096) and 3 01 times in the individuals of T/T genotype of NQO1 gene (95%CI:1 284～9 514) Conclusion:This study suggested that the cDNA609 T allele of NQO1 gene might be the risk factor of lung cancer, which could be associated with genetic susceptibility of lung cancer
Expressing and bioactivity analysis of shark hepatic stimulate substance analogue in Escherichia coli

After being induced with1mmol/LIPTG,the recombinational protein was purified by Gel chromotograˉbing and the activity analyzed with MTT,the prokargotic expression plasmid of sharks hepatic stimulant analogues gene was constructed and transformed into Escherichia coli BLZL line. The results showthat the quantities of recombinant protein to the about38%of the total soluble proteins of BL21under the induction of IPTG,its molecular weight is about17500u analyzed by SDS-PAGE electrophoresis.The purified products can improve the proliferation of SMMC7721cell under the concentration of50ng/L,but will inhibit the cell's proliferation up to100ng/L.Therefore,the recombinant product of shark hepatic stimulant substance analogue gene has the similar activities as the natural form purified from shark lives before.
Comparative study on the determination of reducing sugar

Glucose,Xylose,Fucose,Arabinose,N-acetylglucosamine and Glucosamine were determined by the methods of 3-Methyl-2-benzothiazolinonehydrazone(MBTH),3,5-dinitrosalicylic acid(DNS) and potassium ferricyanide.The results indicated that all the methods were with high precision and recovery rate,the MBTH method had the highest sensitivity,followed by the potassium ferricyanide method,the lowest was the DNS method.So the MBTH method was particularly suitable to the micro-determination of reducing sugar.
Migraine in patients with Patent Foramen Ovale: is there a link?

Background: PFO is a hole between right and left atrium, that is normally present in human fetus, but that sometimes persists in adulthood. Migraine is a neurological disorder frequent in adult patients having Patent Foramen Ovale (PFO). Methods and Results: A variety of clinical manifestations, such as migraine (with or without aura), TIA or cryptogenetic stroke can be present in adult patients with PFO persistence. Echographic findings for diagnosis and percutaneous closure with an Amplazer or other devices for migraine improvement were described. Nevertheless, while some studies report a significant improvement of migraine after PFO closure, other trials refer uncertain or negative results on migraine symptoms after the placement of Amplazer or other devices inducing PFO closure. Conclusion: These uncertain results indicate the relationship between PFO and migraine not is clear and the evidences obtained till now testify both for causal or casual account between two events.
Endogenous Interferon-γ, Macrophage Activation, and Murine Host Defense against Acute Infection with Trypanosoma cruzi

: Parenteral interferon-gamma (IFN-gamma) activates murine macrophages to inhibit Trypanosoma cruzi multiplication and diminishes parasitemia and mortality in acute infection. To investigate the role of endogenous IFN-gamma in acute infection, monoclonal antibody to IFN-gamma was injected intraperitoneally into mice. The 6250 neutralizing units given 24 and 96 h after infection reproducibly increased mortality (P less than .05). Histology sections showed markedly more nests of T. cruzi in treated mice. BALB/c, Swiss Webster, C57Bl/6, and C3H/HEN mice were susceptible to the effects of anti-IFN-gamma. Peritoneal macrophages from mice 4 days after infection and a single dose of 6250 units of anti-IFN-gamma had significantly reduced ability to inhibit T. cruzi multiplication. Multiple doses of anti-IFN-gamma delayed but did not prevent macrophage activation. These results indicate the critical role of endogenous IFN-gamma for macrophage activation and host defense against acute T. cruzi infection in mice.
Circulating Tumor Cells in Renal Cell Carcinoma: Recent Findings and Future Challenges

Oncology Unit, Macerata Hospital, Macerata, Italy, 2 Section of Pathological Anatomy, School of Medicine, United Hospitals, Polytechnic University of the Marche Region, Ancona, Italy, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, United States, Department of Pathology and Surgery, Faculty of Medicine, Córdoba, Spain, Division of Oncology, S. Orsola-Malpighi Hospital, Bologna, Italy, Department of Urology, School of Medicine, United Hospitals, Marche Polytechnic University, Ancona, Italy, Medical Oncology, Department of Oncology,
Novel lineage of a green alga and Acremonium stroudii (Ascomycota) sp. nov. reported from Ascension Island

Environmental specimens lining seawater blowholes of Whale Point, Ascension Island in the South Atlantic Ocean were collected (August 2012) and investigated by morphological and molecular techniques. Reported here, Acremonium stroudii (Ascomycota) sp. nov., a filamentous conidia-forming fungus, was the only fungus isolated from the samples collected. Molecular analysis of the material also indicates the presence of a novel species of green algae being present, however, isolation of this alga has not been possible. Instead it appears that this specimen, which belongs to a novel lineage within the Ulvales, sister to the Dilabifilum species, encourages fungal growth in culture and has been shown to form a symbiotic relationship on low nutrition agar plates, supported by investigation through electron microscopy. As no holotype of this species could be isolated in an axenic culture it was not suitable at this point to try to define this alga, especially as no established genus could be attributed. We recommend trying to further sample areas of Ascension Island looking for other members of this green algal lineage, both investigating free-living green algae and those which are found as lichenized photobionts.
The effect of agitation on the control of secondary metabolism in liquid cultures of penicillium expansum

The effect of agitation on secondary metabolism in Penicillium expansum has been investigated by studying primary metabolic enzyme activities, mycelial lipid accumulation, and the production of the polyketides patulin and citrinin. The results presented here clearly show that agitation directed the flux of acetyl CoA into lipid accumulation rather than polyketide biosynthesis, particularly in the malt extract medium used in these studies. This would be a result of citrate accumulation inducing fatty acid synthesis through the increased activity of the Tricarboxylic Acid (TCA) cycle through increased O2 concentrations. High glucose concentrations in the Yeast Extract Glucose Buffer (YEGB) medium would result in catabolite repression of this activity, and O2 diffusion to the mycelium would have been reduced through the thick mycelial suspensions that formed in the shake cultures. The supply of acetyl CoA to polyketide synthesis, controlled through the availability of O2 to respiratory processes, is considered a major regulatory factor in this organism. The availability of reducing power in NADPH was also a significant factor favouring lipid synthesis over polyketides in shake cultures. The pentose phosphate pathway was more active in shake cultures than static, as were the other potential sources of NADPH in the cytoplasm malic enzyme and the NADP+-utilising form of isocitrate dehydrogenase. The ammonium concentration was not likely to repress polyketide synthesis in either medium. There was some evidence to suggest that patulin synthesis was affected by feedback inhibition of late pathway metabolites or by intracellular proteas~ activity. Finally, the presence of O2 also affected the route taken by intermediates of patulin biosynthesis. The presence of toluquinol in the shake culture filtrates was possibly due to O2 activation of a mixed function oxidase competing for m-cresol with another producing m-hydroxybenzyl alcohol, normally present in static cultures.
Factors associated with unfavorable outcome in minor ischemic stroke

Objective To investigate the risk factors for unfavorable outcome in patients with minor ischemic stroke. Methods Patients with minor ischemic stroke were enrolled prospectively. The modified Rankin Scale (mRS) was used to assess the clinical outcome at day 90 after onset, and mRS 0-2 was defined as favorable outcome. The demographic data, vascular risk factors, clinical data, imaging data, stroke etiologic subtypes, laboratory test results, and treatment methods in the favorable outcome group and unfavorable outcome group were compared. Multivariate logistic regression analysis was used to identify the independent risk factors for early poor outcome in patients with minor ischemic stroke. Results A total of 516 patients with minor ischemic stroke were enrolled. At day 90 after onset, 90 patients (17.44%) had unfavorable outcome and 426 (82.56%) had favorable outcome. Multivariate logistic regression analysis showed that age (odds ratio [OR] 1.045, 95% confidence interval [CI] 1.017-1.074; P=0.002), heart diseases (OR 2.021, 95% CI 1.063-3.841; P=0.032), baseline National Institutes of Health Stroke Scale (NIHSS) score (OR 1.662, 95% CI 1.177-2.347; P=0.004), limb movement disorder (OR 2.430, 95% CI 1.010-5.850; P=0.048), ataxia (OR 2.929, 95% CI 1.188-7.221; P=0.020), early neurological deterioration (OR 50.994, 95% CI 17.659-147.258; P<0.001), infarct diameter (OR 1.279, 95% CI 1.075-1.521; P=0.005), non-responsible vascular stenosis (OR 2.518, 95% CI 1.145-5.536; P=0.022), and large artery atherosclerotic stroke (OR 2.010, 95% CI 1.009-4.003; P=0.047) were the independent risk factors for unfavorable outcome in minor ischemic stroke. Conclusions The early poor outcome of minor ischemic stroke is closely associated with age, heart diseases, baseline NIHSS score, limb movement disorder, ataxia, early neurological deterioration, infarct diameter, non-responsible vascular stenosis, and large artery atherosclerotic stroke. The relevant examinations need to be improved early, the etiologic subtype should be identified, and the correct clinical treatment should be guided. Key words: Stroke; Brain Ischemia; Severity of Illness Index; Magnetic Resonance Imaging; Treatment Outcome; Risk Factors
Life of Photosynthetic Complexes in the Cyanobacterium Synechocystis sp. PCC 6803

The cyanobacterial SCPs consist of one-helix proteins that resemble transmembrane regions of the light-harvesting proteins of plants. To determine whether these proteins are associated with protein complexes in the thylakoid membrane, an abundant member of the SCP family, ScpD, was marked with a His tag, and proteins co-isolating with Histagged ScpD were identified. These proteins included the major PSII components as well as FtsH, which is involved in degradation of the PSII complex. To ascertain specific interaction between ScpD and the PSII complex, the His-tagged protein fraction was subjected to two-dimensional blue native/SDS-PAGE. Again, PSII components were coisolated with ScpD-His, and ScpD-His was found to interact most strongly with CP47. ScpD association was most prominent with the monomeric form of PSII, suggesting ScpD association with PSII that is being repaired. Using antibodies that recognize both ScpC and ScpD, we found the ScpC protein, which is very similar in primary structure to ScpD, to also co-isolate with the PSII complex. In contrast, ScpE did not co-isolate with a major protein complex in thylakoids. A fourth member of the SCP family, ScpB, could not be immunodetected, but was found by mass spectrometry in samples co-isolating with ScpD-His. Therefore, ScpB may be associated with ScpD as well. No association between SCPs and PSI could be demonstrated. On the basis of these and other data presented, we suggest that members of the SCP family can associate with damaged PSII and can serve as a temporary pigment reservoir while PSII components are being replaced. (Published in J. Biol. Chem. 282, 267-276, 2007)
Effectiveness of oyster spat stick communities in removing pharmaceutical contaminants from tidal creeks

Pharmaceuticals are biologically active molecules, designed to affect biological processes at very low doses. Exposure to these compounds poses risks to both aquatic organisms and human health. Faulty septic tanks in coastal areas may release these chemicals into aquatic environments. Field and controlled laboratory studies explored the accumulation of five pharmaceuticals by oysters (Crassostrea virginica). Three sites in Coastal Georgia showed significant (p < 0.05) seasonal variation of pharmaceutical and lipid concentrations in oyster tissue. Controlled laboratory studies indicated a significant (p < 0.05) increase in uptake over 96 h exposure, as well as microbial interactions. The presence of an algal food source significantly (p < 0.05) decreased ibuprofen concentrations in oysters. This study confirms the ability of oysters to assimilate pharmaceuticals associated with human sewage pollution and may vary by seasonal fluctuations in lipid content. INDEX WORDS: Pharmaceuticals, Crassostrea virginica, Septic tanks, Ibuprofen, Diphenhydramine EFFECTIVENESS OF OYSTER SPAT STICK COMMUNITIES IN REMOVING PHARMACEUTICAL CONTAMINANTS FROM TIDAL CREEKS