Miracle-dz/UniFAR-FTUs huggingface dataset:
Structure and expression of c-rel, the cellular homolog to the oncogene of reticuloendotheliosis virus strain T

The cellular homolog of the onc sequences in the avian retrovirus reticuloendotheliosis virus strain T (v-rel) was studied by molecular cloning and nucleic acid hybridization. In contrast to v-rel sequences, which are 1.4 kilobase pairs long, the cellular homolog, c-rel, from line 15B chickens is at least 25 kilobase pairs long, with multiple apparent introns. A 4.0-kilobase polyadenylic acid-containing RNA transcript is the primary species of c-rel RNA present in uninfected chicken cells. Sequences at the 3' end of this c-rel RNA are not present in v-rel.
Effect of radio link and network layer parameters on performance of Zolertia Z1 motes based 6LoWPAN

Unbeknownst the characteristics of radio link parameters and their effect on network performance it becomes difficult to work around the various constraints in low power lossy networks also known as IPv6 over Low Power Wireless Personal Area Networks (6LoWPAN). Based on thorough experiments carried out on the test bed of real zolertia Z1 motes enabled with contiki operating system, the effect of link and network layer parameters on 6LoWPAN performance is analyzed. The performance of 6LoWPAN depends firstly on link metrics viz; radio signal strength indication (RSSI), packet reception ratio (PRR), packet error rate (PER) and link quality indication (LQI). Secondly on node metrics viz; energy consumption, memory occupancy and thirdly on network level metric viz; routing protocol convergence time, control overhead. It is found that the RPL protocol convergence time depends on both the link metrics RSSI and the channel check rate (CCR). Most importantly the node metric; energy consumption is found to be inversely proportional to the link metric RSSI. It is observed that the energy consumption in radio listening state is higher on an average by the factors of 11.64 and 24.66 than in radio transmitting and processing states respectively.
The ALMA Band 9 receiver : Design, construction, characterization, and first light

Aims. We describe the design, construction, and characterization of the Band 9 heterodyne receivers (600–720 GHz) for the Atacama Large Millimeter/submillimeter Array (ALMA). First-light Band 9 data, obtained during ALMA commissioning and science verification phases, are presented as well. Methods. The ALMA Band 9 receiver units (so-called “cartridges”), which are installed in the telescope’s front end, have been designed to detect and down-convert two orthogonal linear polarization components of the light collected by the ALMA antennas. The light entering the front end is refocused with a compact arrangement of mirrors, which is fully contained within the cartridge. The arrangement contains a grid to separate the polarizations and two beam splitters to combine each resulting beam with a local oscillator signal. The combined beams are fed into independent double-sideband mixers, each with a corrugated feedhorn coupling the radiation by way of a waveguide with backshort cavity into an impedance-tuned superconductor-insulator-superconductor (SIS) junction that performs the heterodyne down-conversion. Finally, the generated intermediate frequency (IF) signals are amplified by cryogenic and room-temperature HEMT amplifiers and exported to the telescope’s IF back end for further processing and, finally, correlation. Results. The receivers have been constructed and tested in the laboratory and they show an excellent performance, complying with ALMA requirements. Performance statistics on all 73 Band 9 receivers are reported. Importantly, two di_erent tunnel-barrier technologies (necessitating di_erent tuning circuits) for the SIS junctions have been used, namely conventional AlOx barriers and the more recent high-current-density AlN barriers. On-sky characterization and tests of the performance of the Band 9 cartridges are presented using commissioning data. Continuum and line images of the low-mass protobinary IRAS 16293-2422 are presented which were obtained as part of the ALMA science verification program. An 8 GHz wide Band 9 spectrum extracted over a 0:300 _0:300 region near source B, containing more than 100 emission lines, illustrates the quality of the data.
Impaired Insulin Signaling in Skeletal Muscles from Transgenic Mice Expressing Kinase-deficient Insulin Receptors (*)

Transgenic mice which overexpress kinase-deficient human insulin receptors in muscle were used to study the relationship between insulin receptor tyrosine kinase and the in vivo activation of several downstream signaling pathways. Intravenous insulin stimulated insulin receptor tyrosine kinase activity by 7-fold in control muscle versus ≤1.5-fold in muscle from transgenic mice. Similarly, insulin failed to stimulate tyrosyl phosphorylation of receptor β-subunits or insulin receptor substrate 1 (IRS-1) in transgenic muscle. Insulin substantially stimulated IRS-1-associated phosphatidylinositol (PI) 3-kinase in control versus absent stimulation in transgenic muscles. In contrast, insulin-like growth factor 1 modestly stimulated PI 3-kinase in both control and transgenic muscle. The effects of insulin to stimulate p42 mitogen-activated protein kinase and c-fos mRNA expression were also markedly impaired in transgenic muscle. Specific immunoprecipitation of human receptors followed by measurement of residual insulin receptors suggested the presence of hybrid mouse-human heterodimers. In contrast, negligible hybrid formation involving insulin-like growth factor 1 receptors was evident. We conclude that (i) transgenic expression of kinase-defective insulin receptors exerts dominant-negative effects at the level of receptor autophosphorylation and kinase activation; (ii) insulin receptor tyrosine kinase activity is required for in vivo insulin-stimulated IRS-1 phosphorylation, IRS-1-associated PI 3-kinase activation, phosphorylation of mitogen-activated protein kinase, and c-fos gene induction in skeletal muscle; (iii) hybrid receptor formation is likely to contribute to the in vivo dominant-negative effects of kinase-defective receptor expression.
Oxygen accelerates the accumulation of mutations during the senescence and immortalization of murine cells in culture

Oxidative damage is a causal factor in aging and cancer, but it is still not clear how DNA damage, the cellular responses to such damage and its conversion to mutations by misrepair or misreplication contribute to these processes. Using transgenic mice carrying a lacZ mutation reporter, we have previously shown that mutations increase with age in most organs and tissues in vivo. It has also been previously shown that mouse cells respond to oxidative stress, typical of standard culture conditions, by undergoing cellular senescence. To understand better the consequences of oxidative stress, we cultured mouse embryo fibroblasts (MEFs) from lacZ mice under physiological oxygen tension (3%) or the high oxygen tension (20%) associated with standard culture, and determined the frequency and spectrum of mutations. Upon primary culture, the mutation frequency was found to increase approximately three‐fold relative to the embryo. The majority of mutations were genome rearrangements. Subsequent culture in 20% oxygen resulted in senescence, followed by spontaneous immortalization. Immortalization was accompanied by an additional three‐fold increase in mutations, most of which were G:C to T:A transversions, a signature mutation of oxidative DNA damage. In 3% oxygen, by contrast, MEFs did not senesce and the mutation frequency and spectrum remained similar to primary cultures. These findings demonstrate for the first time the impact of oxidative stress on the genomic integrity of murine cells during senescence and immortalization.
Incidence, recurrence, and long-term survival of ischemic stroke subtypes: A population-based study in the Middle East

Background Incidence, risk factors, case fatality and survival rates of ischemic stroke subtypes are unknown in the Middle East due to the lack of community-based incidence stroke studies in this region. Aim To characterize ischemic stroke subtypes in a Middle Eastern population. Methods The Mashad Stroke Incidence Study is a community-based study that prospectively ascertained all cases of stroke among the 450,229 inhabitants of Mashhad, Iran between 2006 and 2007. We identified 512 cases of first-ever ischemic stroke [264 men (mean age 65.5 ± 14.4) and 248 women (mean age 64.14 ± 14.5)]. Subtypes of ischemic stroke were classified according to the TOAST criteria. Incidence rates were age standardized to the WHO and European populations. Results The proportion of stroke subtypes was distributed as follows: 14.1% large artery disease, 15% cardioembolic, 22.5% small artery disease, 43.9% undetermined and 4.5% other. The greatest overall incidence rates were attributed to undetermined infarction (49.97/100,000) followed by small artery disease (25.54/100,000). Prevalence of hypertension, diabetes and atrial fibrillation differed among ischemic stroke subtypes. Overall, there were 268 (52.34%) deaths and 73 (14.25%) recurrent strokes at five years after incident ischemic stroke, with the greatest risk of recurrence seen in the large artery disease (35.6%) and cardioembolic (35.5%) subgroups. Survival was similar in men and women for each stroke subtype. Conclusions We observed markedly greater incidence rates of ischemic stroke subtypes than in other countries within the Mashad Stroke Incidence Study after age standardization. Our findings should be considered when planning prevention and stroke care services in this region.
High frame rate ultrasound imaging of human carotid artery dynamics

The carotid artery (CA) is one of the main arteries of interest to cardiovascular research, because of the clinical relevance of CA plaques as culprits of stroke and the accessibility of the CA for cardiovascular health screening. Viscoelastic properties of the arterial wall and of possible lesions within are key components for clinical evaluation of the CA. These viscoelastic properties can be assessed by monitoring the interaction of the wall with a passing arterial pulse wave using ultrasound (US) imaging. The local wall motion (position, velocity, and acceleration) may contain high frequency components and hence a full understanding of tissue mechanics in the CA requires very high frame rate imaging. In this paper we present a high density motion analysis of the CA based on an arterial pulse wave imaged with 2.5 up to 35 kHz frame rate US imaging.
A Dynamic View on the Financing of Extreme Growth Companies

This paper researches the determinants of financing decisions of extreme growth companies. For this purpose, we use a longitudinal dataset, free of survivorship bias, covering the financing events of extreme growth companies for up to eight years. Results are generally consistent with the extended pecking order theory. Profitable companies are more likely to use internal finance, while they have unused debt capacity, which is contrary to the predictions of the static trade-off theory. External equity is used as a last resort. Nearly 20% of the firms issuing external equity have a negative shareholders' equity in the previous year. Finally, consistent with the static trade-off theory, companies with a higher probability and costs of financial distress and higher agency costs are more likely to issue external equity financing.
Breakthrough Technologies New Generation of Arti fi cial MicroRNA and Synthetic TransActing Small Interfering RNA Vectors for Ef fi cient Gene Silencing in Arabidopsis 1

Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are used for small RNA-based, specific gene silencing or knockdown in plants. Current methods to generate amiRNA or syn-tasiRNA constructs are not well adapted for cost-effective, large-scale production or for multiplexing to specifically suppress multiple targets. Here, we describe simple, fast, and cost-effective methods with high-throughput capability to generate amiRNA and multiplexed syn-tasiRNA constructs for efficient gene silencing in Arabidopsis (Arabidopsis thaliana) and other plant species. amiRNA or syn-tasiRNA inserts resulting from the annealing of two overlapping and partially complementary oligonucleotides are ligated directionally into a zero background BsaI/ ccdB-based expression vector. BsaI/ccdB vectors for amiRNA or syn-tasiRNA cloning and expression contain a modified version of Arabidopsis MIR390a or TAS1c precursors, respectively, in which a fragment of the endogenous sequence was substituted by a ccdB cassette flanked by two BsaI sites. Several amiRNA and syn-tasiRNA sequences designed to target one or more endogenous genes were validated in transgenic plants that (1) exhibited the expected phenotypes predicted by loss of target gene function, (2) accumulated high levels of accurately processed amiRNAs or syn-tasiRNAs, and (3) had reduced levels of the corresponding target RNAs.
Compact UWB MIMO antenna with WiMAX and WLAN rejection

An Ultrawideband (UWB) MIMO antenna capable of covering 3.1 to 10.6 GHz frequency band having compact size is presented. To avoid severe electromagnetic interference from the narrow band services, band rejection characteristics is provided at 3.3 to 3.7 GHz WiMAX band and 5.15 to 5.85 GHz WLAN band. The proposed antenna gives good isolation of less than -20 dB at all frequencies. Results show that the H plane radiation pattern is omni-directional and antenna gain is almost stable in the entire operating frequency range. Envelope correlation coefficient less than 0.008 ensures better diversity performance.
Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization.

We have investigated the excision of a variety of modified bases from DNA by the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase) [Boiteux, S. , O'Connor, T. R. , Lederer, F. , Gouyette, A. , & Laval, J. (1990) J. Biol. Chem. 265, 3916-3922]. DNA used as a substrate was modified either by exposure to ionizing radiation or by photosensitization using visible light in the presence of methylene blue (MB). The technique of gas chromatography/mass spectrometry, which can unambiguously identify and quantitate pyrimidine- and purine-derived lesions in DNA, was used for analysis of hydrolyzed and derivatized DNA samples. Thirteen products resulting from pyrimidines and purines were detected in gamma-irradiated DNA, whereas only the formation of 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) and 8-hydroxyguanine (8-OH-Gua) was observed in visible light/MB-treated DNA. Analysis of gamma-irradiated DNA after incubation with the Fpg protein followed by precipitation revealed that the Fpg protein significantly excised 4,6-diamino-5-formamidopyrimidine (FapyAde), FapyGua, and 8-OH-Gua. The excision of a small but detectable amount of 8-hydroxyadenine was also observed. The detection of these products in the supernatant fractions of the same samples confirmed their excision by the enzyme. Nine pyrimidine-derived lesions were not excised. The Fpg protein also excised FapyGua and 8-OH-Gua from visible light/MB-treated DNA. The presence of these products in the supernatant fractions confirmed their excision. (ABSTRACT TRUNCATED AT 250 WORDS)
Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences.

The 5.8S gene and flanking internal transcribed spacers (ITS1 and ITS2) of the rDNA were amplified from total DNA extracted from frond tissues of Livistona chinensis with universal and fungal-specific primers. These amplified fragments were cloned and sequenced. Phylogenetic analysis based on the 5.8S gene sequences indicated that the six clone sequences obtained were of different origins. Five sequences, P1-9, P2-6, P4-4, P4-5, and P4-7, belonged to the fungi and one sequence, P3-2, belonged to the plants. P1-9 was inferred to belong to the Basidiomycota based on the phylogenetic analysis of the 5.8S gene sequences but could not be identified to lower taxonomic levels. Further identification of the other four fungal clones to lower taxonomic levels was attempted based on phylogenetic analysis and sequence comparison of both the conserved 5.8S gene and the variable ITS regions. The origin of P2-6 was identified to be Glomerella and its anamorph Colletotrichum, the origins of P4-5 and P4-7 were Mycosphaerella and its anamorph Cladosporium, and the origin of P4-4 was the Herpotrichiellaceae. The direct approach to detection and taxonomic placement of endophytic fungi within host tissue without the need for conventional in vitro culturing is discussed.
Precision Nanomedicine Using Dual Positron Emission Tomography and Magnetic Resonance Temperature Imaging – Guided Photothermal Therapy

Precision Nanomedicine Using Dual Positron Emission Tomography and Magnetic Resonance Temperature Imaging–Guided Photothermal Therapy Min Zhou, Marites Melancon, R. Jason Stafford, Junjie Li, Alpa M. Nick, Mei Tian, Anil K. Sood, and Chun Li* Departments of Cancer Systems Imaging, Interventional Radiology, Imaging Physics, Gynecologic Oncology and Reproductive Medicine, and Center for RNAi and NonCoding RNA, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, United States
INVESTIGATION OF SURVIVAL, GROWTH AND BIOCHEMICAL BLOOD PARAMETERSOF COMMON CARP (CYPRINUS CARPIO L.) LARVAE FED BY ARTIFICIAL DIETS

Culture of fish larvae is one of the most sensitive stages in production of most fish species. The most important problem is providing suitable and high quality feed which is acceptable, digestible, causes lower mortality and higher growth. In this study three diets (SFK, industrial granules feed), (SFK+ Enzyme (Lipase, Pepsin, Trypsin)) and Gammarus were examined. Each experiment carried out in triplicate with duration of 30 days. The 2400 larvae of common carp with average weight of 5±0.5mg were used for each treatment. Biometry of fishes was conducted every 6 days and data of weight, length, food conversion ratio, specific growth rate and survival were recorded. At the end of the trial, blood sampling was conducted with incision of tail peduncle for analyzing of biochemical blood parameters. Results showed that the larvae fed with SFK+Enzyme had better and significant growth than others (p 0.05).
Roboconf : an Autonomic Platform Supporting Multi-level Fine-grained Elasticity of Complex Applications on the Cloud. (Roboconf : une plateforme autonomique pour l'élasticité multi-niveau, multi-granularité pour les applications complexes dans le cloud)

Software applications are becoming more diverse and complex. With the stormy development of Cloud Computing and its applications, software applications become even more complex than ever. The complex Cloud applications may contain a lot of software components that require and consume a large amount of resources (hardware or other software components) distributed into multiple levels based on granularity of these resources. Moreover these software components might be located on different clouds. The software components and their required resources of a Cloud application have complex relationships which some could be resolved at design time but some are required to tackle at run time. The complexity of software and heterogeneity of Cloud environment become challenges that current elasticity solutions need to find appropriate answers to resolve. Elasticity is one of benefits of Cloud computing, which is capability of a Cloud system to adapt to workload changes by provisioning and deprovisioning resources in an autonomic manner. Hence, the available resources fit the current demand as closely as possible at each point in time. To have an efficient elasticity solution which not only reflects the complexity of Cloud applications but also deploy and manage them in an autonomic manner, we propose a novel elasticity approach. It is called multi-level fine-grained elasticity which includes two aspects of application’s complexity: multiple software components and the granularity of resources. The multi-level fine-grained elasticity concerns objects impacted by elasticity actions and granularity of these actions. In this thesis, we introduce Roboconf platform an autonomic Cloud computing system (ACCS) to install and reconfigure the complex applications as well as support the multi-level fine-grained elasticity. To this end, Roboconf is also an autonomic elasticity manager. Thanks to this platform, we can abstract the complex Cloud applications and automate their installation and reconfiguration that can be up to several hundred hours of labour. We also use Roboconf to implement the algorithms of multi-level fine-grained elasticity on these applications. The conducted experiments not only indicate efficiency of the multi-level fine-grained elasticity but also validate features supporting this approach of Roboconf platform.
Teletutoring over the BETEL network

We will describe the multimedia teletutoring prototype jointly developed by EPFL and Eurecom in the context of BETEL, using the state of the art technology available in 1993. With the aide of high quality videoconferencing and shared workspace tools, the actual experiment was successfully carried out at the end of the year over one of the first 34 Mbps Trans-European ATM networks interconnecting sites in France and Switzerland. This network was called the Broadband Exchange over Trans-European Links (BETEL). The aim of this paper is to describe the BETEL teletutoring platform and scenarios, together with limitations and future enhancements of this prototype. Focus is placed on the interactive audio and video communications part of the application.
DNA relatedness and serotyping of Leptospira strains.

BACKGROUND In a previous study (5), the constructed phylogenetic tree for leptospires belonging to 12 different serovars common in Central Europe made the prediction of serovar from knowing the genotype and vice versa possible. OBJECTIVE The study was aimed at investigation of the usefulness of such procedure to distinguish in between at present to us available and worldwide accepted reference strains of pathogenic Leptospira serovars. MATERIAL AND METHODS One hundred and seventy seven Leptospira strains representing different serovars were tested. DNA fingerprints of these strains were performed, digitally captured and as described earlier of those phylogenetic tree using different fingerprinting software was constructed using UPGMA clustering method with band matching by the Dice coefficient (5). RESULTS At this tree, 145 of 177 Leptospira strains tested each took a unique position, and the remaining 32 strains were distributed at 15 different positions (each of 14 positions taken by two different strains and one position taken by four strains). CONCLUSION The constructed phylogenetic tree likely to be very useful in prediction of Leptospira serovar in most cases of an infection so the saving time and being helpful in serovar identification of the pathogenic agent (Fig. 1, Ref. 9).