Miracle-dz/UniFAR-FTUs huggingface dataset:
The Fate of PrP GPI‐Anchor Signal Peptide is Modulated by P238S Pathogenic Mutation

Glycosylphosphatidylinositol (GPI)‐anchored proteins are localized to the plasma membrane via a C‐terminally linked GPI anchor. The GPI anchor is added concomitantly to the cleavage of the carboxy‐terminal GPI‐anchor signal sequence, thereby causing the release of a C‐terminal hydrophobic peptide, whose fate has not yet been investigated. Here we followed the fate of the GPI‐attachment signal of the prion protein (PrP), a protein implicated in various types of transmissible neurodegenerative spongiform encephalopathies (TSE). The PrP GPI‐anchor signal sequence shows a remarkable and unusual degree of conservation across the species and contains two point mutations (M232R/T and P238S) that are responsible for genetic forms of prion disorders. We show that the PrP GPI‐anchor signal peptide (SP), but not the one from an unrelated GPI‐anchored protein (folate receptor), undergoes degradation via the proteasome. Moreover, the P238S point mutation partially protects the PrP GPI‐anchor SP from degradation. Our data provide the first attempt to address the fate of a GPI‐anchor SP and identify a role for the P238S mutation, suggesting the possibility that the PrP GPI‐anchor SP could play a role in neurodegenerative prion diseases.
Keep Winning or Stop Losing ? The Effect of Consumption Outcomes on Variety-Seeking in Online Video Games

As consumers engage in experiential products, they may exhibit variety-seeking behavior by switching frequently between different options within the same category, or exhibit inertial behavior by consistently choosing the same option. The prior literature identifies three main reasons why consumers may be variety-seeking: external situations, satiation, and future preference uncertainty. We propose that consumers may also become variety-seeking in response to consumption outcomes, which indicate the quality of the consumer’s experience for a specific consumption occasion. We predict that positive consumption outcomes lead to inertial preferences, while negative consumption outcomes lead to variety-seeking. We test our hypothesis within the novel context of an online video game, in which players choose between different map environments for each round of play and consumption outcomes can be measured objectively by a player’s performance during the round. In our preliminary model, we extend upon classic models of variety-seeking that view the choices made between consumption occasions as a first order Markov process. We allow variety-seeking to be a function of time-varying factors, including satiation and consumption outcomes. We observe the effects of consumption outcomes on variety-seeking to be consistent with our hypothesis, which suggests in our context that firms should place players in a familiar environment in the next round of play if they are performing well, and introduce variety if they are performing poorly.
Large-eddy Simulation of Flow Separation Control by Spanwise Alternatively Distributed Strips Control

An implicit large-eddy simulation (ILES) study is presented for turbulent boundary layer separation from a backward-facing rounded ramp with active wall actuations control. This method, namely spanwise alternatively distributed strips (SADS) control, is imposed onto the flat plate surface upstream of the ramp by alternatively applying out-of-phase control (OPC) and in-phase control (IPC) to the wall-normal velocity component in the lateral direction. As a result, the local turbulence is alternatively suppressed by OPC strips and enhanced by IPC strips, leading to the creation of a vertical shear layer, which is responsible for the generation of the large-scale streamwise vortices (LSSVs). These LSSVs, thought to be similar to Prandtl’s second kind of secondary flow, can be further sustained by the SADS control, exerting a predominant influence on the suppression of the flow separation. The interaction among the LSSV, the downstream recirculation zone and free-shear layer is studied by examining flow statistics, including skin-friction, wall pressure coefficients, skin friction lines, mean streamwise velocity, turbulent kinetic energy and Reynolds stress. It is found that the flow separation is delayed by the SADS control and the size of the mean recirculation zone is reduced.
DELIVERABLE D 8 . 5 : Public report on final conclusions

Project Acronym: NanoHybrids Project Name: New generation of nanoporous organic and hybrid aerogels for industrial applications: from laboratory to pilot scale production Call: Manufacturing and control of nanoporous materials (H2020NMP-PILOTS-2015) Project Type: Innovation actions Grant Agreement No.: 685648 – NanoHybrids – H2020-NMP-2014-2015/H2020NMPPILOTS-2015 Project Start Date: 1 November 2015 Project Duration: 42-Months Due date of Deliverable: Month 42, 30/04 /2019 Final Version Date: Month 42, 30/04 /2019 Name of Lead Beneficiary for this Deliverable: TUHH, Germany
Laceration of the eye with a fishing hook

Editor,—We report a case of late onset of Leber’s hereditary optic neuropathy (LHON) in a 59 year old patient with HIV infection being treated long term since 1991 with zidovudine. The onset and course of the patient’s eye disease as well as the diagnostic process were analysed. Molecular genetic testing revealed the mitochondrial DNA (mtDNA) mutation in nucleotide position 11778 confirming the diagnosis of LHON. In our patient late disease expression may be interpreted as a result of the rare combination of the 11778 mutation with HIV infection and long term zidovudine treatment. This is the first report on a patient with LHON suffering from additional HIV infection. ### CASE REPORT A 59 year old white man was admitted to our outpatient department in July 1997 with acute onset of vision loss in his left eye within the previous 2 weeks. The …
In vitro study of injury on human bronchial epithelial cells caused by gunpowder smog

Abstract Smog inhalation is associated with acute respiratory symptoms in exposed victims. However, despite the evidence from cell injury caused by smog, a stable and practical apparatus used to treat cells with smog is necessary. The aim of this study is to develop a cell research platform of smoke inhalation injury. In the smog-generation device, a wireless electromagnetic heater was used to ignite gunpowder and generate smog. The quality of black powder was checked by the black powder burn rate, and experimental smog was indirectly checked by the amount of cell damage. The temperature and humidity were set at 37 °C ± 1 °C and ≥95% in the smog-cells reaction chamber, respectively. Factors including gunpowder dosages, smog-exposure time, the cell density, modes of exposure, volumes of smog, test durations, volumes of the cell culture medium and combustion velocity were measured. Coefficient variation of different batches of gunpowder and smog were less than 4% and 9%, respectively. With larger gunpowder dosage and longer exposure time, cell injury appeared to increase. When cells were cultured in 4 × 104/well density in culture medium (1 mL/well), exposed to more than 10 L smog with filter screens above plates, detected after 24 h culture in cell incubator and gunpowder burned out within 5 s, smog had the best effect on cell injury. In conclusion, the experimental device can produce test smog stably and safely. The apparatus treating cells with smog can induce cell injury effectively, and the injury is positively correlated with smog concentration and exposure time.
Implementation of Artistic Curly Hair Dynamics in Houdini

This is an implementation of artistic curly hair dynamics according to the method introduced by Pixar to achieve the artistic hair introduced in the movie Brave. This implementation is done using Houidini, to give Houdini users the ability to apply these dynamics to their hair easily. The implementation is divided into two major sections, the dynamics of a single strain of hair, and optimizing the collision between diﬀerent hair strains. For the single strain, a combination of three strings is introduced, Stretching string, Bending string, and Core string. For collisions, the operation is optimized by pruning the close particles in the one strain to reduce the amount of particles processed for a single strain from a side, and then from the other side, neighboring hair strains are pruned when possible to reduce the amount of strains to process.
AN EFFICIENT IN-VITRO AGROBACTERIUM-MEDIATED TRANSFORMATION PROTOCOL FOR RAISING SALINITY TOLERANT TRANSGENIC PLANTS IN FINGERMILLET [ ELEUSINE CORACANA ( L . ) GAERTN . ]

An efficient reproducible protocol has been established for raising salinity tolerant fingermillet through in vitro Agrobacterium mediated transformation. To enhance the salinity tolerance in fingermillet a double gene construct of PgNHX1 (from Pennisetum glaucum) and AVP1 (from Arabidopsis thaliana) was developed using the plant binary expression vector pCAMBIA 1301. This was then mobilized into electro competent Agrobacterium tumefaciens strain EHA105 and used for transforming fingermillet var. GPU28. GUS histochemical assay was monitored for confirmation of callus transformation. The putative transformants were acclimatized in incubation chamber for two weeks and then transplanted in greenhouse for further acclimatization. Putative transgenics were confirmed by physiological analysis and PCR amplification of genomic DNA using primers. The putative transgenic plants showed higher salt tolerance of 300mM compared to treated wild type plants. This is the first study in fingermillet reporting the expression of double gene construct of PgNHX1 and AVP1 for salinity tolerance.
Audio-Visual Speech Activity Detector

Cílem této práce je vytvo it audio-vizuální detektor e i, t.j. algoritmus, kter ̋ automaticky identifikuje, zda osoba ve videozáznamu v danou chvíli mluví. Tato úloha je d leûitá pro tzv. audio-vizuální diarizaci nebo audio-vizuální rozpoznávání a u ení identit. Navrûen ̋ detektor e i má dv fáze. V první fázi je e detekována pouze na základ vizuální informace. V druhé ásti jsou jiû detekované ásti videa testovány na synchronnost s audio signálem. Z lokalizovan ̋ch v ̋znamn ̋ch bod na detekované tvá i jsou extrahovány geometrické video p íznaky. Mel-frequency cepstral coe cients jsou pouûity jako audio p íznaky. Synchronnost audio a video p íznak je testována kanonickou korela ní anal ̋zou s fixními projek ními koeficienty. Tento algoritmus je schopen vizuáln detekovat e a spolehliv ov it synchronnost na dostate n dlouhé ásti videozáznamu, podle experiment alespo 8 sekund.
PREDICTIVE URBAN ANALYTICS Exploring Choice Modelling and Revealed Preferences for Urban Design

Since the rise of generative design, a morphogenetic process of designing has emerged where algorithms are used to explore potential permutations of a solution to find the best design option. Yet, on a subjective level, identifying what option is considered best has often proven to be difficult. Hence, the paper discusses a foundation research to investigate prototypically subjective judgments concern matters of value and preference defined by end users in generativemodelled urban design outcome. To do so the paper will introduce and outline research findings in the field of Micro-Economics, in particular its subcategories ‘Choice Modelling’ as a method and ‘Revealed Preferences’ as a methodology to assess whether user preferences can be identified and engaged as ‘preferred’ design options. In the paper the research will outline in greater depth the theories behind Choice Modelling and Revealed Preferences, a field that studies the behaviour of individuals, and its relevance for urban design, in particular Computational Urbanism. The paper discusses how Choice Modelling can analyse design outcomes and conclude and speculate about its use in an applied context.
The blitz, civilian morale and the city: mass-observation and working-class culture in Britain, 1940–41

One of the most difficult concepts in both contemporary and academic accounts of the Second World War is that of civilian morale. This paper uses evidence from the Mass-Observation Archive to argue that understanding fluctuations in morale can only be understood through an exploration of working-class culture during the 1930s and 1940s. The paper examines difficulties of defining ‘morale’ and goes on to argue that the pattern of bombing in urban centres and the continuity of working-class institutions helped shape and maintain morale during the critical period of 1940–41
Bulk acoustic wave resonators and filters - 2d modelling and industrialization aspects

A two dimensional model for Bulk Acoustic Wave (BAW) resonators and filters is applied to predict electrical behaviour for various configurations. The results are explained in terms of lateral mode excitation at the electrode edge. It is found that both the vertical and lateral particle displacement should be considered to achieve realistic results, especially for the case of thick top electrodes. Furthermore the industrialization of the solidly mounted bulk acoustic wave filters within Philips Semiconductors is discussed. The industrial flow towards a wafer-level chip-scale packaged product for the USPCS standard is described. The characteristics of the first samples are close to the product specifications.
AUTOMATED PROCESSING OF DIGITAL IMAGE DATA IN ARCHITECTURAL SURVEYING

Digital image maps can be produced by assuming an object surface and using the eight-parameter transformation for photo rectification or with knowledge about image orientation and a given object model.The latter is known as an orthophoto where the object model is represented by a digital terrain model. In close-range architectural applications the hidden-area problem has to be solved. A building structure can be recorded by means of multi-image photogrammetry with several photos. Orientation of the images has to be carried out by bundle adjustment. The result provides photo positions and some object points. Additional line measurements define a geometrical frame. This wire-frame model can be automatically transfered into a surface structure. The orthogonal projection of digital image data takes into consideration the hidden parts of the object and is getting the gray values from photos with the best intersection. Furthermore the information about texture orientation for ray-tracing programs can be generated. Algorithms
Enteral nutrition tube placement assisted by ultrasonography in patients with severe acute pancreatitis

Abstract To evaluate the effect of a novel procedure using real-time ultrasonography to assist nasojejunal tube placement at bedside in patients with severe acute pancreatitis (SAP). Single center, prospective descriptive study in a 15-bed surgery intensive care unit of a university hospital. Thirty SAP patients were enrolled. The whole procedure of placing nasojejunal tube was performed by a single physician, who places nasojejunal tube at the bedside and performs ultrasonography to guide the tube positioning. The final nasojejunal tube position was confirmed by abdominal radiograph. The successful rate of the procedure as well as the time it took, the time from the decision of enteral feeding to commencement of feeding, and complications were recorded. Thirty-six intubations were performed in 30 patients by using ultrasonography-assisted method at bedside. Nasojejunal tubes were successful placed in 28 of 30 patients (93.3%). The average time of successful placement was 22.07 ± 5.78 minutes. The median time between physician's decision for tube placement and feeding initiation was 5.5 (2, 24) hours. No adverse events occurred in all of patients. This novel method of nasojejunal tube placement under ultrasound guidance is practical, less time consuming and reliable.
MORTAR DURABILITY – DEVELOPMENT AND STANDARDIZATION OF TEST METHODS

Many factors influence mortar durability, some of which are related to environmental and in-service conditions, which should be designed for, and some of which are related to the properties of the masonry units, the cement mortar and their interactions. Because of the large number of factors influencing durability, it has been difficult in the past to develop test methods. There have therefore been no standard performance criteria for durability of mortar. This paper briefly reviews mechanisms of deterioration of masonry mortars and describes work carried out to develop laboratory and field test methods to assess the service life of masonry mortars subjected to salt crystallisation, abrasion and wind action. The paper describes the adoption of the field test into the current Australian masonry standard and the performance criteria adopted.
Optimal Prediction Intervals of Wind Power Generation

Accurate and reliable wind power forecasting is essential to power system operation. Given significant uncertainties involved in wind generation, probabilistic interval forecasting provides a unique solution to estimate and quantify the potential impacts and risks facing system operation with wind penetration beforehand. This paper proposes a novel hybrid intelligent algorithm approach to directly formulate optimal prediction intervals of wind power generation based on extreme learning machine and particle swarm optimization. Prediction intervals with associated confidence levels are generated through direct optimization of both the coverage probability and sharpness to ensure the quality. The proposed method does not involve the statistical inference or distribution assumption of forecasting errors needed in most existing methods. Case studies using real wind farm data from Australia have been conducted. Comparing with benchmarks applied, experimental results demonstrate the high efficiency and reliability of the developed approach. It is therefore convinced that the proposed method provides a new generalized framework for probabilistic wind power forecasting with high reliability and flexibility and has a high potential of practical applications in power systems.
Chemical Composition and Antinociceptive Potential of Campomanesia reitziana Fruits.

The methanolic extract from Campomanesia reitziana fruits and the main active principle, identified as 4',6'-dihydroxy-3',5'-dimethyl-2'-methoxy chalcone or dimethyl cardamonin (1), exhibited pronounced antinociceptive effects against two models of pain in mice. Compound 1 caused dose-dependent inhibition of abdominal constrictions, with a calculated ID50 value of 8.1 (6.5-10.1) μmol/kg (i.p.), being about 16-fold more potent than two reference analgesic drugs. Methanolic extract and 1 were also effective against the formalin model, inhibiting both phases of pain, causing reductions of 39.9% and 26.8% (extract, 10 mg/kg) and 52.9% and 57.6% (compound 1, 5 mg/kg) for the first and second phases, respectively.
Noninvasive Ultrasound Monitoring of Embryonic and Fetal Development in Chinchilla lanigera to Predict Gestational Age: Preliminary Evaluation of This Species as a Novel Animal Model of Human Pregnancy

Ultrasound is a noninvasive routine method that allows real-time monitoring of fetal development in utero to determine gestational age and to detect congenital anomalies and multiple pregnancies. To date, the developmental biology of Chinchilla lanigera has not yet been characterized. This species has been found to undergo placentation, long gestation, and fetal dimensions similar to those in humans. The aim of this study was to assess the use of high-frequency ultrasound (HFUS) and clinical ultrasound (US) to predict gestational age in chinchillas and evaluate the possibility of this species as a new animal model for the study of human pregnancy. In this study, 35 pregnant females and a total of 74 embryos and fetuses were monitored. Ultrasound examination was feasible in almost all chinchilla subjects. It was possible to monitor the chinchilla embryo with HFUS from embryonic day (E) 15 to 60 and with US from E15 to E115 due to fetus dimensions. The placenta could be visualized and measured with HFUS from E15, but not with US until E30. From E30, the heartbeat became detectable and it was possible to measure fetal biometrics. In the late stages of pregnancy, stomach, eyes, and lenses became visible. Our study demonstrated the importance of employing both techniques while monitoring embryonic and fetal development to obtain an overall and detailed view of all structures and to recognize any malformation at an early stage. Pregnancy in chinchillas can be confirmed as early as the 15th day postmating, and sonographic changes and gestational age are well correlated. The quantitative measurements of fetal and placental growth performed in this study could be useful in setting up a database for comparison with human fetal ultrasounds. We speculate that, in the future, the chinchilla could be used as an animal model for the study of US in human pregnancy.
Horned Grebe Podiceps auritus and Red-necked Grebe Podiceps grisegena in Sweden 2011 – results from a national survey

For conservation purposes it is important to track the status and trend of a given population. Trends can be estimated when the size of the population is regularly estimated (Sutherland 2006). A common approach for bird population monitoring is to take repeatable sample measurements, either in the breeding areas (e.g. line transects or point counts), along the migratory pathway (e.g. standardized ringing or observation schemes), or during winter for birds forming dense flocks (e.g. counting numbers: Bibby et al. 2000). Breeding bird censuses are per definition spatially linked to a certain region, which is a great advantage when monitoring a local population. But surveying a breeding species over a vast area is often demanding and costly. Observation schemes along the migratory pathway are relatively cheap and can therefore be carried out regularly, and a relative measure of the amount of birds passing can be summarized on a yearly basis. The apparent weakness with this method is that the population size and the true distribution of the birds counted rarely are known. Two sparsely occurring bird species in Sweden are the Horned Grebe Podiceps auritus and the Horned Grebe Podiceps auritus and Red-necked Grebe Podiceps grisegena in Sweden 2011 – results from a national survey
The Effects Of Planting Density , Cutting Stage and Cutting Number On Yield and Quality Of Stevia rebaudiana

To avoid harmful health effects of synthetic sugar and also due to human attention to supply sugar from natural sources, the interests of the sugar plants cultivation, especially Stevia rebaudiana have been increased. A field experiment was conducted to study the effect of planting density, cutting stage and cutting number on Stevia. In Çukurova region, which is classified as Mediterranean climate. The experiment conducted in a split plot design consisted of three planting density, 30 × 60, 45 × 60 and 60 × 60 cm in the main plot and two cutting time in various growth stages in subplots with three replications. Since there were two cuttings in the season, the differences between cuttings, statistical analysis was valued by using split-split plot design in time. Planting density significantly affected the dry leaf yield and the higher yield (110.9 kg da1) was obtained from the plants, spaced 30 × 60 cm. Plants cutting at flowering stage had high dry leaf weight (20.55 g plant-1) and yield (84.70 kg da-1). Also, more dry leaf weight and yield were obtained from first cuttings. Leaves of closer plant densities produced high content of stevioside. Pre-flowering stage had significant effect on stevioside in the affirmative way.