Miracle-dz/UniFAR-FTUs huggingface dataset:
Super-Resolution Imaging of Fas/CD95 Reorganization Induced by Membrane-Bound Fas Ligand Reveals Nanoscale Clustering Upstream of FADD Recruitment

Signaling through the TNF-family receptor Fas/CD95 can trigger apoptosis or non-apoptotic cellular responses and is essential for protection from autoimmunity. Receptor clustering has been observed following interaction with Fas ligand (FasL), but the stoichiometry of Fas, particularly when triggered by membrane-bound FasL, the only form of FasL competent at inducing programmed cell death, is not known. Here we used super-resolution microscopy to study the behavior of single molecules of Fas/CD95 on the plasma membrane after interaction of Fas with FasL on planar lipid bilayers. We observed rapid formation of Fas protein superclusters containing more than 20 receptors after interactions with membrane-bound FasL. Fluorescence correlation imaging demonstrated recruitment of FADD dependent on an intact Fas death domain, with lipid raft association playing a secondary role. Flow-cytometric FRET analysis confirmed these results, and also showed that some Fas clustering can occur in the absence of FADD and caspase-8. Point mutations in the Fas death domain associated with autoimmune lymphoproliferative syndrome (ALPS) completely disrupted Fas reorganization and FADD recruitment, confirming structure-based predictions of the critical role that these residues play in Fas–Fas and Fas–FADD interactions. Finally, we showed that induction of apoptosis correlated with the ability to form superclusters and recruit FADD.
Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement

Advances in molecular technologies over the past few decades, such as high-throughput DNA marker genotyping, have provided more powerful plant breeding approaches, including marker-assisted selection and genomic selection. At the same time, massive investments in plant genetics and genomics, led by whole genome sequencing, have led to greater knowledge of genes and genetic pathways across plant genomes. However, there remains a gap between approaches focused on forward genetics, which start with a phenotype to map a mutant locus or QTL with the goal of cloning the causal gene, and approaches using reverse genetics, which start with large-scale sequence data and work back to the gene function. The recent establishment of efficient CRISPR-Cas-based gene editing promises to bridge this gap and provide a rapid method to functionally validate genes and alleles identified through studies of natural variation. CRISPR-Cas techniques can be used to knock out single or multiple genes, precisely modify genes through base and prime editing, and replace alleles. Moreover, technologies such as protoplast isolation, in planta transformation, and the use of developmental regulatory genes promise to enable high-throughput gene editing to accelerate crop improvement.
A worker-centered-model to understand the factors influencing knowledge application

Individuals represent the link between knowledge management initiatives and firm performance because the decision of applying knowledge depends ultimately on them. However, most of the studies describing barriers in knowledge management do not explicitly analyze the barriers that individuals face during the process of knowledge application. In this paper, we present a model of knowledge processes for the knowledge worker, the worker-centered model (WCM). The WCM represents the factors influencing knowledge application during knowledge intensive activities from the point of view of the knowledge worker. Three main knowledge processes are identified and the influent factors are allocated one to one to them. This transparent representation of the factors-process relations simplifies the analysis of failures in knowledge application. The identification of the failure factors from the individuals’ perspective is key to understand the requirements for improved methods of knowledge management that support more extensive and efficient knowledge application.
Pelaksanaan Program e KTP Dalam Rangka Tertib Administrasi Kependudukan

Dengan berlakunya Undang – undang Nomor 23 Tahun 2006 tentang Administasi Kependudukan dengan tujuan untuk mewujudnya tertib administrasi kependudukan dalam sekala Nasional dan menjamin kepastian hukum hak sipil penduduk, juga untuk kepentingan program – program pembangunan Nasional, sehingga diperlukan pemutakhiran data kependudukan, penerbitan Nomor Induk Kependudukan (NIK) dan penerapan e-KTP. KTP Elektronik adalah dokumen kependudukan yang memuat sistem keamanan / pengendalian baik dari sisi administrasi ataupun teknologi informasi dengan berbasis pada database kependudukan nasional. Dengan tujuan untuk mewujudkan kepemilikan satu identitas (KTP) untuk satu penduduk yang memiliki kode keamanan dan rekaman elektronik data kependudukan berbasis NIK secara Nasional. (biodata,foto,sidik jari, iris mata dan tanda tangan) yang tersimpan dalam fisik KTP Elektronik (KTP Elektronik). Kendala-kendala yang timbul pada pelaksanaan Program e-KTP yaitu datangnya alat perekam data yang tidak bisa tepat waktu karena alatnya yang datang tidak bisa serentak dan alatnya juga ada yang tidak lengkap serta tidak bisa dioperasionalkan juga belum dapat diserahkannya e-KTP kepada penduduk tepat waktu karena belum seluruhnya e-KTP selesai dicetak karena pencetakannya dilaksanakan oleh konsursium yang berada di Jakatra sehingga perlu adanya perbaikan-perbaikan pelayanan yang diberikan oleh pemerintah, agar masyarakat mendapatkan pelayanan yang maksimal dalam pembuatan e-KTP Kata Kunci: UU no 23 Tahun 2006, e-KTP
Granular discrete element modelling : packing of non-spherical granular particles

• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers.
Toward a Systems Framework Coupling Safety Culture, Risk Perception, and Hazard Recognition for the Mining Industry

The United States mining industry has made steady progress to improve worker safety and reduce injuries. Despite these gains, the industry remains largely reactive in its approach to health and safety. There remains a primary focus on lagging indicators, such as the numbers of injuries, hours lost, and hazards found at the worksite. To facilitate a more proactive approach, new methods are needed to evaluate hazardous conditions and unsafe behaviors. This work explores the relationships among mine workers’ hazard recognition abilities, the individual’s perception of risk, and the safety culture of the mining workplace. We have conducted a literature review to identify key factors and analytical models in industries where health and safety are a major consideration, including construction, manufacturing, mining, and transportation. Our analysis considered both process-oriented frameworks, such as Systems Thinking approaches, and statistical methods, including Structural Equation Modeling (SEM). A meta-model was then developed to aggregate and examine key factors and potential causal relationships. We discuss the creation of this meta-model, identifying notable structural characteristics and hypotheses for future confirmatory analysis. Use cases are then outlined, including descriptive, evaluative, and generative applications.
Ethics Education in Business Schools: Emerging Issues and Challenges

Ethical education is a perpetual learning process, lasting from childhood until the end of life. Human beings continually learn about ethical values, behavioural justification, and moral conscience, whether it is from the family, which is the basic and fundamental cell of society in its entirety, or from politics or one’s workplace. University students who prepare for an occupation with a high level of social responsibility have a chance to acquire knowledge about morality, ethics, and values. Managers and those studying management also need to consider ethics. Ethics education proves to be vital in various professions, and the learning process in this field could be more effective in the training programmes of those professions. Universities which offer management programmes include ethics education and teach the knowledge and skills of ethical behaviour, which students go on to practice in their respective workplaces. The question, however, is, “What does this school offer in ethics education? ” The main goal of this article is to explore which areas of ethics in tertiary education in the Czech Republic are offered in management degrees, and how this is connected with true ethical leadership. Keywords: Education, ethics, ethical leadership, management
Environmental Management Accounting (EMA) practices and plastic pollution control in selected food and beverage firms

Abstract This study investigated the relationship between EMA and plastic pollution control. The study concentrated on Food and Beverage manufacturing companies since they use plastic goods to package their products. Plastic products are discarded once they have served their purpose, which pollutes the environment. A quantitative approach was adopted, with 124 self-completed questionnaires being administered to financial managers, management accountants, factory accountants and chief accountants of 38 selected food and beverage manufacturing firms in Durban. The findings revealed a significant relationship between environmental information and environmentally-related activities. Environmental information also had a positive relationship with corporate environmental strategy. Moreover, a strong positive relationship was discovered between environmental information and plastic pollution control. An analysis of the link between environmentally-related activities and plastic pollution control revealed an insignificant relationship. An insignificant relationship was also discovered between corporate environmental strategy and plastic pollution control. This study suggests that companies within the Food and Beverage industry must put in place sustainable environmental strategies, whereby eco-friendly disposal initiatives must be implemented for plastic waste management.
Regulation of the Intestinal Extra-Adrenal Steroidogenic Pathway Component LRH-1 by Glucocorticoids in Ulcerative Colitis

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) and can be treated with glucocorticoids (GC), although some patients are unresponsive to this therapy. The transcription factor LRH-1/NR5A2 is critical to intestinal cortisol production (intestinal steroidogenesis), being reduced in UC patients. However, the relationship between LRH-1 expression and distribution with altered corticosteroid responses is unknown. To address this, we categorized UC patients by their steroid response. Here, we found that steroid-dependent and refractory patients presented reduced glucocorticoid receptor (GR)-mediated intestinal steroidogenesis compared to healthy individuals and responder patients, possibly related to increased colonic mucosa GR isoform beta (GRβ) content and cytoplasmic LRH-1 levels in epithelial and lamina propria cells. Interestingly, an intestinal epithelium-specific GR-induced knockout (GRiKO) dextran sodium sulfate (DSS)-colitis mice model presented decreased epithelial LRH-1 expression, whilst it increased in the lamina propria compared to DSS-treated control mice. Mechanistically, GR directly induced NR5A2 gene expression in CCD841CoN cells and human colonic organoids. Furthermore, GR bound to two glucocorticoid-response elements within the NR5A2 promoter in dexamethasone-stimulated CCD841CoN cells. We conclude that GR contributes to intestinal steroidogenesis by inducing LRH-1 in epithelial cells, suggesting LRH-1 as a potential marker for glucocorticoid-impaired response in UC. However, further studies with a larger patient cohort will be necessary to confirm role of LRH-1 as a therapeutic biomarker.
Feasibility of spent macroalgae biochar for removal of Acid Red 88 (AR) dye from its aqueous solution

Biochar was synthesized from Kappaphycus alvarezii reject (KR) remove the anionic azo dye, Acid Red 88 from wastewater. In this study, biochar was experimented in both batch and column adsorption studies. Batch adsorption studies were performed at varying temperature (25 – 40 °C), dosage (1 – 10 g), pH (4 – 10), time (30 – 90 min) and dye concentration (25 – 125 mg/L) respectively. From the batch adsorption studies, it was seen that maximum removal efficiency was 87.64% at 75ppm, dosage of 4 g/L, time of 60 min and pH 7 respectively. The batch adsorption experiment best fitted to Freundlich isotherm model with Pseudo-second-order kinetics indicating the adsorption to follow chemisorption mechanism process along with multilayer adsorption onto biochar’s uneven adsorption sites. It was clear from this study, that Kappaphycus alvarezii reject has the potential to serve as economical biochar as it can be utilized as an adsorbent for the efficient removal of colorants from wastewater.
Use of the Manufacturing Execution System in Practice: Understanding the end Users and the Acceptance of the System

Purpose – this paper aims to examine the factors that affect the acceptance and use of the Manufacturing Execution System from the point of view of end users, using the Unified Theory of Acceptance and Use of Technology (UTAUT) model. Theoretical framework - The technology acceptance model is based on principles adopted in the attitude paradigm of psychology, which specifies how to measure the relevant attitudinal components of behavior; distinguishes beliefs and attitudes and specific as external stimuli, the objective characteristics of an attitude object, are causally linked to beliefs, attitudes and behavior. Design/methodology/approach - Data were collected by the survey method, using the seven-point Likert scale. The approach was quantitative, using the Structural Equation Modeling technique, using SPSS V.25 software. Findings - The findings of this study showed that the Facilitating Conditions influence the Usage Behavior of users of the Manufacturing Execution System. The application of multi-group comparison analysis (MGA) showed that Effort Expectancy positively influences Behavioral Intention and Social Influence also positively affects Behavioral Intention. Research, Practical & Social implications - The study brings relevant contributions in relation to aspects of understanding about the level of acceptance of MES end users in the operation phase. Originality/value - This paper makes a contribution to the literature due to the pioneering nature of the research, since no research was found in academic bases that address the use of UTAUT with the MES system. Another important contribution is that this study proposes a new model, based on UTAUT using the application of MGA. Keywords - Acceptance and Use. Technology. Manufacturing Execution System.
Thiamine Level in Out-of-hospital Cardiac Arrest Patients

BACKGROUND: Thiamine deficiency is more common in critically ill patients. Administration of thiamine in cardiac arrest mice has improved survival and neurological outcomes. Evidence for thiamine deficiency in cardiac arrest humans is insufficient to support routine use of thiamine in cardiac arrest patients. AIM: This study aimed to determine thiamine blood levels in cardiac arrest patients to understand whether the presence of thiamine deficiency is common in cardiac arrest patients. METHODS: A prospective descriptive study from April 2017 to March 2018, on 24 adult out-of-hospital cardiac arrest patients. We used the high-performance liquid chromatography technique to determine whole blood thiamine pyrophosphate levels in cardiac arrest patients who arrived at the emergency department within 1 h of the onset of a cardiac arrest. RESULTS: The mean thiamine pyrophosphate level within 1 h of the onset of a cardiac arrest was 170.9 ± 56.7 nmol/L. Only one participant had thiamine deficiency according to the cut-off level for thiamine pyrophosphate in whole blood of <70 nmol/L. Fourteen patients had spontaneous return of circulation. Thiamine pyrophosphate levels were not different between the two groups of patients who had and did not have the return of spontaneous circulation at the emergency department. CONCLUSION: Little evidence was found to support thiamine deficiency as a feature among our cardiac arrest patients. A study with a larger population is required for more meaningful statistical analysis. As there is no consensus on cut-off level for thiamine deficiency diagnosis, the level of thiamine pyrophosphate in specific populations should be evaluated to establish reference values.
Formation and Thermal Stability of the ω-Phase in Ti–Nb and Ti–Mo Alloys Subjected to HPT

This paper discusses the features of ω-phase formation and its thermal stability depending on the phase composition, alloying element and the grain size of the initial microstructure of Ti–Nb and Ti–Mo alloys subjected to high-pressure torsion (HPT) deformation. In the case of two-phase Ti–3wt. % Nb and Ti–20wt. % Nb alloys with different volume fractions of α- and β-phases, a complete β→ω phase transformation and partial α→ω transformation were found. The dependence of the α→ω transformation on the concentration of the alloying element was determined: the greater content of Nb in the α-phase, the lower the amount of ω-phase that was formed from it. In the case of single-phase Ti–Mo alloys, it was found that the amount of ω-phase formed from the coarse-grained β-phase of the Ti–18wt. % Mo alloy was less than the amount of the ω-phase formed from the fine α′-martensite of the Ti–2wt. % Mo alloy. This was despite the fact that the ω-phase is easier to form from the β-phase than from the α- or α′-phase. It is possible that the grain size of the microstructure also affected the phase transformation, namely, the fine martensitic plates more easily gain deformation and overcome the critical shear stresses necessary for the phase transformation. It was also found that the thermal stability of the ω-phase in the Ti–Nb and Ti–Mo alloys increased with the increasing concentration of Nb or Mo.
Genetic Variability and Performance of MPOB-Nigeria Dura x AVROS Pisifera Planting Materials

A long-term evaluation on fourteen D x P progenies of introgressed MPOB-Nigeria dura x AVROS pisifera were laid down in a randomized complete block design in two replicates with 371 palms in 2002. The aim was to evaluate the performance of fresh fruit bunch yield, bunch quality and vegetative traits among progenies. Analysis of variance revealed highly significant difference for all traits, indicating the existence of substantial variability within these population. In all cases, the phenotypic coefficient of variance was higher than the genotypic coefficients. Broad-sense heritability values estimated ranged from 8.85% to 100% for all the traits studied. The fresh fruit bunch also was found to be positively and highly correlated with bunch number, average bunch weight, oil yield ratio and kernel yield ratio. PK 3248 and PK 3166 were preferred as potential parental lines in breeding programmes and to be included in introgression with advanced breeding populations such as Deli dura.
Pedagogical foundations of the problem of forming communicative competence of future elementary school teachers

The article is devoted to the problem of forming communicative competence of future elementary school teachers. The objectives of the study were to highlight the conceptual pedagogical foundations and components on which the process of forming communicative competence in future elementary school teachers is based. The task of scientific exploration was to conduct a pilot study to identify the level of understanding of the future elementary school teachers of the phenomenon of “communicative competence” and its importance for further professional activity. The organized research was based on theoretical (study, analysis, systematization, and generalization of scientific-pedagogical, psychological, and methodological literature on the problem under study) and empirical (diagnostic evaluation, questioning, monitoring and educational experiment) methods. The concepts of “competence”, “competence”, “communicative competence”, “communicative competence of a future elementary school teacher” were revealed. The problem of development of communicative competence as a professional value of a modern elementary school teacher is outlined. The results of the survey of students-future teachers of primary classes on the understanding of the essence and necessity of formation of communicative competence during professional training in higher educational institutions of Ukraine are presented.
Nanostructured Layer of Silver for Detection of Small Biomolecules in Surface-Assisted Laser Desorption Ionization Mass Spectrometry

A facile approach for the synthesis of a silver nanostructured layer for application in surface-assisted laser desorption/ionization mass spectrometry of low-molecular-weight biomolecules was developed using electrochemical deposition. The deposition was carried out using the following silver salts: trifluoroacetate, acetate and nitrate, varying the voltage and time. The plate based on trifluoroacetate at 10 V for 15 min showed intense SALDI-MS responses for standards of various classes of compounds: fatty acids, cyclitols, saccharides and lipids at a concentration of 1 nmol/spot, with values of the signal-to-noise ratio ≥50. The values of the limit of detection were 0.71 µM for adonitol, 2.08 µM for glucose and 0.39 µM for palmitic acid per spot. SEM analysis of the plate showed anisotropic flower-like microstructures with nanostructures on their surface. The reduced chemical background in the low-mass region can probably be explained by the absence of stabilizers and reducing agents during the synthesis. The plate synthesized with the developed approach showed potential for future use in the analysis of low-molecular-weight compounds of biological relevance. The absence of the need for the utilization of sophisticated equipment and the synthesis time (10 min) may benefit large-scale applications of the layer for the detection of various types of small biomolecules.