Miracle-dz/UniFAR-FTUs huggingface dataset:
Factors Related to Internet Adoption and Its Use to Seek Health Information in Mexico

ABSTRACT The Internet has the potential to be a valuable resource for the dissemination of health promotion information to the general population, mainly in conditions with well-developed health and digital literacy. Few studies have been undertaken on the adoption of the Internet by the Mexican population and its use to seek health information. The aim of this paper was to identify the factors that determine Internet adoption and its use by the heads of Mexican households for obtaining health information. This study used data taken from a probabilistic and cross-sectional national survey (National Survey on the Availability and Use of Information Technologies in Households, or ENDUTIH), applying an economic approach based on utility maximization theory. We estimated a univariate probit model for Internet adoption and a consecutive bivariate probit model with sample selection for the use of the Internet to seek health information. The software package was used to adjust and estimate the proposed models. The first model (Internet adoption) identified several factors related to digital divides in the country, while the second (Internet use to seek health information) identified various factors influencing online searches for health information, such as the following: being a woman; being an adult; having a higher level of education; having a higher income; having superior digital skills; and, living in an urban area. This study highlights the need to strengthen digital policy in order to improve access to and the adoption and efficient use of the Internet, particularly in terms of improving an individual’s engagement with their own health.
OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease

The hepatitis C virus (HCV) nonstructural protein 3-4A (NS3-4A) protease is a key component of the viral replication complex and the target of protease inhibitors used in current clinical practice. By cleaving and thereby inactivating selected host factors it also plays a role in the persistence and pathogenesis of hepatitis C. Here, we describe ovarian cancer immunoreactive antigen domain containing protein 1 (OCIAD1) as a novel cellular substrate of the HCV NS3-4A protease. OCIAD1 was identified by quantitative proteomics involving stable isotopic labeling using amino acids in cell culture coupled with mass spectrometry. It is a poorly characterized membrane protein believed to be involved in cancer development. OCIAD1 is cleaved by the NS3-4A protease at Cys 38, close to a predicted transmembrane segment. Cleavage was observed in heterologous expression systems, the replicon and cell culture-derived HCV systems, as well as in liver biopsies from patients with chronic hepatitis C. NS3-4A proteases from diverse hepacivirus species efficiently cleaved OCIAD1. The subcellular localization of OCIAD1 on mitochondria was not altered by NS3-4A-mediated cleavage. Interestingly, OCIAD2, a homolog of OCIAD1 with a cysteine residue in a similar position and identical subcellular localization, was not cleaved by NS3-4A. Domain swapping experiments revealed that the sequence surrounding the cleavage site as well as the predicted transmembrane segment contribute to substrate selectivity. Overexpression as well as knock down and rescue experiments did not affect the HCV life cycle in vitro, raising the possibility that OCIAD1 may be involved in the pathogenesis of hepatitis C in vivo.
Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding

Simple Summary Hot-iron disbudding in calves and goat kids is a widespread husbandry procedure involving pain and, especially in goats, the risk of brain damage. The injection of clove oil under the horn bud is a potential alternative disbudding method. Clove oil, with its active substance eugenol, is cytotoxic but has anaesthetic effects, and the injection of clove oil, or the pure substance isoeugenol, under the horn bud to stop horn growth may have potential welfare benefits compared to hot-iron disbudding. We compared the injection of clove oil with the injection of isoeugenol under the horn bud with respect to pain sensitivity in this area in the first 24 h after injection. Compared to before injection, the pain sensitivity of goats kids was higher 24 h after injection of clove oil and up to at least 6 h after isoeugenol injection, while, in calves, pain sensitivity was highest after 9 h. Future studies should compare the welfare effects of isoeugenol injection with hot-iron disbudding. Abstract In this preliminary study, we compared changes in mechanical nociceptive thresholds (MNT) of calves and goat kids injected with clove oil or isoeugenol under the horn bud as a potential, more welfare-friendly alternative to hot-iron disbudding. Twenty male calves and goat kids were randomly allocated to clove oil (n = 10 per species) or isoeugenol (n = 10 per species) injection under the horn buds. MNT was measured via a pressure algometer in calves and kids at several locations around the horn buds at several time points before and up to 24 h after injection. In kids, von Frey filaments were used additionally at the same time points. In calves, linear mixed models revealed an effect on MNT of time point (p = 0.010) and side (p = 0.007), but not of injection (p = 0.298), nor of the interaction ‘injection*time point’, MNT waslowest 9 h post-injection. In goats, there was an effect of injection depending on time point (interaction injection*time point, p = 0.03) with MNT being lowest 24 h post-injection for clove oil, while MNT was similar to pre-injection in isoeugenol. In both species, variation in the individual response post-injection was very high. Our results suggest that clove oil and isoeugenol induced hypersensitivity, which was higher for clove oil, in goat kids, but they also suggest a transient anaesthetic effect in some animals and locations.
Estimation of Microbial Viability Using Flow Cytometry

For microorganisms in particular, viability is a term that is difficult to define and a state consequently difficult to measure. The traditional (and gold‐standard) usage equates viability and culturability (i.e., the ability to multiply), but the process of determining culturability is often too slow. Flow cytometry provides the opportunity to make rapid and quantitative measurements of dye uptake in large numbers of cells, and we can therefore exploit the flow cytometric approach to evaluate so‐called viability stains and to develop protocols for more routine assessments of microbial viability. This unit is primarily commentary, but several basic protocols have been included to ensure that users have a firm basis for attempting these reasonably difficult assays on traditional flow cytometer instruments. What is clear is that each assay must be carefully validated with the particular microorganism of interest before being applied in any research, clinical, or service form.
Influence of FTO (Fat mass and obesity) gene and parental obesity on Brazilian children and adolescents adiposity

Abstract Objectives Evaluate the influence of the genetic variant rs9939609 of the FTO gene on anthropometric characteristics and whether parental obesity is related to children and adolescents being overweight. Methods A total of 2,364 children and adolescents between 6 and 17 years old were genotyped and the lipid profile, plasma glucose level, and anthropometric characteristics were measured to assess adiposity. Results The AA genotype (risk) was associated with higher body mass index (BMI Z-score; p = 0.006), waist circumference (WC; p = 0.001), and triglycerides (p = 0.033). The association of the participants’ adiposity characteristics with the parents’ BMI and FTO genotypes showed an association of the BMI Z-score when either the mother or father was overweight or obese (p = 0.028 and p = 0.029). In the overweight or obese father/eutrophic mother, we also observe an association of FTO rs9939609 with WC (p = 0.039). The effect of these variables on the risk of obesity was also tested: overweight or obese mother (OR = 1.82, p = 0.041), overweight and obese parents (OR = 3.09, p < 0.0001), and FTO rs9939609 AA genotype (OR = 2.08, p = 0.0004) were associated. With regard to altered WC and high body fat percentage (BF%), either overweight or obese parents (OR = 2.39, p < 0.0001; OR = 1.92, p < 0.002) showed an association. The FTO rs9939609 AA genotype (OR = 1.99, p = 0.0002) was associated with altered WC. Conclusions The results show that parental weight also contributes to obesity and may interact with the FTO genetic make-up.
A new genomic taxonomy system for the Synechococcus collective.

Cyanobacteria of the genus Synechococcus are major contributors to global primary productivity and are found in a wide range of aquatic ecosystems. This Synechococcus collective (SC) is metabolically diverse, with some lineages thriving in polar and nutrient-rich locations and others in tropical or riverine waters. Although many studies have discussed the ecology and evolution of the SC, there is a paucity of knowledge on its taxonomic structure. Thus, we present a new taxonomic classification framework for the SC based on recent advances in microbial genomic taxonomy. Phylogenomic analyses of 1085 cyanobacterial genomes demonstrate that organisms classified as Synechococcus are polyphyletic at the order rank. The SC is classified into 15 genera, which are placed into five distinct orders within the phylum Cyanobacteria: i. Synechococcales (Cyanobium, Inmanicoccus, Lacustricoccus gen. nov., Parasynechococcus, Pseudosynechococcus, Regnicoccus, Synechospongium gen. nov., Synechococcus, and Vulcanococcus); ii. Cyanobacteriales (Limnothrix); iii. Leptococcales (Brevicoccus and Leptococcus); iv. Thermosynechococcales (Stenotopis and Thermosynechococcus), and v. Neosynechococcales (Neosynechococcus). The newly proposed classification is consistent with habitat distribution patterns (seawater, freshwater, brackish, and thermal environments) and reflects the ecological and evolutionary relationships of the SC. This article is protected by copyright. All rights reserved.
Cutting Edge Molecular Therapy for Acute Myeloid Leukemia

Recently, whole exome sequencing for acute myeloid leukemia (AML) has been performed by a next-generation sequencer in several studies. It has been revealed that a few gene mutations are identified per AML patient. Some of these mutations are actionable mutations that affect the response to an approved targeted treatment that is available for off-label treatment or that is available in clinical trials. The era of precision medicine for AML has arrived, and it is extremely important to detect actionable mutations relevant to treatment decision-making. However, the percentage of actionable mutations found in AML is about 50% at present, and therapeutic development is also needed for AML patients without actionable mutations. In contrast, the newly approved drugs are less toxic than conventional intensive chemotherapy and can be combined with low-intensity treatments. These combination therapies can contribute to the improvement of prognosis, especially in elderly AML patients who account for more than half of all AML patients. Thus, the treatment strategy for leukemia is changing drastically and showing rapid progress. In this review, we present the latest information regarding the recent development of treatment for AML.
Development and Characterization of Potential Ocular Mucoadhesive Nano Lipid Carriers Using Full Factorial Design

Generally, topically applied eye drops have low bioavailability due to short residence time and low penetration of the drug. The aim of the present study was to incorporate dexamethasone (DXM) into nano lipid carriers (NLC), which contain mucoadhesive polymer, in order to increase the bioavailability of the drug. A 23 factorial experimental design was applied, in which the three factors were the polymer, the DXM, and the emulsifier concentrations. The samples were analyzed for particle size, zeta potential, polydispersity index, and Span value. The significant factors were identified. The biocompatibility of the formulations was evaluated with human corneal toxicity tests and immunoassay analysis. The possible increase in bioavailability was analyzed by means of mucoadhesivity, in vitro drug diffusion, and different penetration tests, such as in vitro cornea PAMPA model, human corneal cell penetration, and ex vivo porcine corneal penetration using Raman mapping. The results indicated that DXM can be incorporated in stable mucoadhesive NLC systems, which are non-toxic and do not have any harmful effect on cell junctions. Mucoadhesive NLCs can create a depot on the surface of the cornea, which can predict improved bioavailability.
Estimating global biodiversity: tropical beetles and wasps send different signals

New Perspectives is intended to allow the communication of comments, viewpoints, and speculative interpretation of issues in ecology pertinent to entomology. Comments, viewpoints, or suggestions arising from published papers intended to fuel discussion and debate are also welcome. Contributions should be as concise as possible, normally not exceeding 2000 words. Formal research reports will not be acceptable, but summarized novel data, suitably supported by statistics, may be allowed.
Evaluation of LoRa Technology in Flooding Prevention Scenarios

Global climate change originates frequent floods that may cause severe damage, justifying the need for real-time remote monitoring and alerting systems. Several works deal with LoRa (Long Range) communications over land and in the presence of obstacles, but little is known about LoRa communication reliability over water, as it may happen in real flooding scenarios. One aspect that is known to influence the communication quality is the height at which nodes are placed. However, its impact in water environments is unknown. This is an important aspect that may influence the location of sensor nodes and the network topology. To fill this gap, we conducted several experiments using a real LoRa deployment to evaluate several features related to data communication. We considered two deployment scenarios corresponding to countryside and estuary environments. The nodes were placed at low heights, communicating, respectively, over the ground and over the water. Measurements for packet loss, received signal strength indicator (RSSI), signal-to-noise ratio (SNR) and round-trip time (RTT) were collected during a period of several weeks. Results for both scenarios are presented and compared in this paper. One important conclusion is that the communication distance and reliability are significantly affected by tides when the communication is done over the water and nodes are placed at low heights. Based on the RTT measurements and on the characteristics of the hardware, we also derive a battery lifetime estimation model that may be helpful for the definition of an adequate maintenance plan.
A cost-effective and sensitive TLC-densitometric identification of meloxicam

Abstract The influence of different chromatographic conditions and the process of spot visualization on determining the limit of detection as well as quantification (LOD and LOQ) of meloxicam by TLC-densitometric technique was estimated. Of all chromatographic conditions tested, the lowest limiting values, thus the best sensitivity, in the NP-TLC system was achieved on silica gel 60F254 and neutral aluminum oxide plates developed with the mobile phase consisting of ethyl acetate/toluene/n-butylamine (2:2:1, V/V/V). In the case of the RP-TLC method, a mixture of methanol/water (8:2, V/V) enabled densitometric detection of meloxicam at the lowest concentration level on RP-8F254 and RP-18F254 plates. Additionally, the smallest LOD value of meloxicam ensured crystalline violet and gentian violet as visualization agents on silica gel 60F254 and neutral aluminum oxide 150F254 plates, resp. Comparison of the densitometrically obtained spectra of meloxicam drug and its standard after the use of appropriate visualization agents could be a good and cheap alternative tool for the identification of meloxicam as an active pharmaceutical ingredient.
A Multilayer and Multimodal-Fusion Architecture for Simultaneous Recognition of Endovascular Manipulations and Assessment of Technical Skills

The clinical success of the percutaneous coronary intervention (PCI) is highly dependent on endovascular manipulation skills and dexterous manipulation strategies of interventionalists. However, the analysis of endovascular manipulations and related discussion for technical skill assessment are limited. In this study, a multilayer and multimodal-fusion architecture is proposed to recognize six typical endovascular manipulations. The synchronously acquired multimodal motion signals from ten subjects are used as the inputs of the architecture independently. Six classification-based and two rule-based fusion algorithms are evaluated for performance comparisons. The recognition metrics under the determined architecture are further used to assess technical skills. The experimental results indicate that the proposed architecture can achieve the overall accuracy of 96.41%, much higher than that of a single-layer recognition architecture (92.85%). In addition, the multimodal fusion brings significant performance improvement in comparison with single-modal schemes. Furthermore, the $K$ -means-based skill assessment can obtain an accuracy of 95% to cluster the attempts made by different skill-level groups. These hopeful results indicate the great possibility of the architecture to facilitate clinical skill assessment and skill learning.
Hippocampal circuits underlie improvements in self‐reported anxiety following mindfulness training

Mindfulness meditation has successfully been applied to cultivate skills in self‐regulation of emotion, as it employs the unbiased present moment awareness of experience. This heightened attention to and awareness of sensory experience has been postulated to create an optimal therapeutic exposure condition and thereby improve extinction learning. We recently demonstrated increased connectivity in hippocampal circuits during the contextual retrieval of extinction memory following mindfulness training.
Blood vessels of the dental pulp

The dental pulp occupies a location different from all other soft tissues in the body. Almost entirely enclosed in unyielding walls of dentin, its only connection with other tissues is by minute openings for vessels and nerves. Because of this, its blood supply is of special interest and offers special problems. Anatomical literature gives very little infomiation concerning the mode of entry arid clisti*ihtion of vessels and even less concerning the pliysiology of circulation within this rigid box. The present study was utidertakcn after attempts to explain some of the phenomena of the pulp associated with vascular reaction had failed because of lack of inforination on the distribution of vessels. It was desired to know how many vessels entered and left the pulp, their means of ingress or egress and whether or not there were anastomoses of arteries within the pulp, especially in multirooted teeth.
Patients' perspectives on a new delivery model in primary care: A propensity score matched analysis of patient‐reported outcomes in a Dutch cohort study

Abstract Rationale, aims and objective Primary Care Plus (PC+) focuses on the substitution of hospital‐based medical care to the primary care setting without moving hospital facilities. The aim of this study was to examine whether population health and experience of care in PC+ could be maintained. Therefore, health‐related quality of life (HRQoL) and experienced quality of care from a patient perspective were compared between patients referred to PC+ and to hospital‐based outpatient care (HBOC). Methods This cohort study included patients from a Dutch region, visiting PC+ or HBOC between December 2014 and April 2018. With patient questionnaires (T0, T1 and T2), the HRQoL and experience of care were measured. One‐to‐two nearest neighbour calliper propensity score matching (PSM) was used to control for potential selection bias. Outcomes were compared using marginal linear models and Pearson chi‐square tests. Results One thousand one hundred thirteen PC+ patients were matched to 606 HBOC patients with well‐balanced baseline characteristics (SMDs <0.1). Regarding HRQoL outcomes, no significant interaction terms between time and group were found (P > .05), indicating no difference in HRQoL development between the groups over time. Regarding experienced quality of care, no differences were found between PC+ and HBOC patients. Only travel time was significantly shorter in the HBOC group (P ≤ .001). Conclusion Results show equal effects on HRQoL outcomes over time between the groups. Regarding experienced quality of care, only differences in travel time were found. Taken as a whole, population health and quality of care were maintained with PC+ and future research should focus more on cost‐related outcomes.
A novel single nucleotide polymorphism in exon 4 of insulin-like growth factor-1 associated with production traits in Bali cattle.

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism ( SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa 1 restriction enzyme. Genotypic polymorphism of IGF-1/ Rsa 1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/ Rsa 1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain.
More Vulnerable? The Life Story Approach Highlights Older People’s Potential for Strength During the Pandemic

Abstract Objectives Older adults have repeatedly been referred to as more physically vulnerable during the COVID-19 pandemic. The pandemic, however, is not only about becoming physically ill. It has many psychosocial aspects: people are exposed to myriad life challenges. The life story approach does not ignore physical status but also emphasizes psychosocial strengths. It highlights that older people are likely to have developed resilience through experiencing life challenges and living across history. Method We used the narrative method to review research on three strengths: tendency toward life reflection, adaptive use of personal memory, and temporal focus encouraging generativity. Results For each, we (a) present evidence that this strength manifests in the second half of life, and (b) identify how it may specifically be applied in dealing with the challenges of the pandemic. In considering their life stories, the picture that emerges is one of older adults as having the potential to show considerable psychosocial strength despite the adversities of the pandemic. Discussion We conclude that during this period of sweeping change in the lives of individuals of all ages, our older citizens may act as valuable societal anchors.