Miracle-dz/UniFAR-FTUs huggingface dataset:
A qualitative study examining prosthesis use in everyday life in individuals with lower limb amputations

Background: Lower limb amputation (LLA) is a life-changing event that affects functional mobility and participation in everyday life. Prostheses provide individuals with LLA the opportunity to improve mobility and quality of life; however, existing literature suggests that there is underuse of prostheses. Objectives: The purpose of this study is to describe how individuals with major LLA use their prosthesis in everyday life and describe barriers and facilitators that influence prosthesis use. Study Design: A qualitative descriptive methodology was used. Methods: Ten community dwelling adults (>18 years) with unilateral major LLAs (transtibial, transfemoral, and knee disarticulation) attending outpatient follow-up at a specialized rehabilitation center were recruited. Participants were purposively sampled for variation in prosthesis use and level of amputation. One-on-one semistructured in-depth interviews were conducted in person or by telephone. The interviews were audio recorded, transcribed verbatim, and analyzed inductively using thematic analysis. Results: The experiences of individuals with major LLA were organized into three overarching themes: everyday experiences using a prosthesis, extrinsic factors influencing prosthesis use, and intrinsic factors influencing prosthesis use. These themes, and their associated subthemes, highlight how individuals use their prosthesis and the various factors perceived to act as barriers and facilitators to prosthesis use in everyday life. Conclusions: These findings provide valuable insight into the everyday experiences of individuals with LLA and can be used to implement strategies to optimize prosthesis use.
Collision risk of bats with small wind turbines: Worst-case scenarios near roosts, commuting and hunting structures

Small wind turbines (SWTs) have become increasingly common within the last decade, but their impact on wildlife, especially bats, is largely unknown. We conducted an operational experiment by sequentially placing a mobile SWT with five different operational modes at six sites of high bat activity, including roosts, commuting structures, and highly frequented hunting areas. Bat flight trajectories around the SWT were documented at each site during five consecutive nights using a specifically designed high-spatial-resolution 3D camera. The recordings showed high bat activity levels close to the SWT (7,065 flight trajectories within a 10-m radius). The minimum distance to the rotor of each trajectory varied between 0 and 18 m, with a mean of 4.6 m across all sites. Linear mixed models created to account for site differences showed that, compared to a reference pole without a SWT, bats flew 0.4 m closer to the rotor (95% CI 0.3–0.6 m) if it was out of operation and 0.3 m closer (95% CI 0.1–0.4 m) if it was moving slowly. Exploratory behavior was frequently observed, with many bats deviating from their original flight trajectory to approach the rotor. Among 7,850 documented trajectories, 176 crossed the rotor, including 65 while it was in motion. The collision of one P. pygmaeus individual occurred during the experiment. These results demonstrate that, despite the generally strong ability of bats to evade moving rotor blades, bat casualties at SWTs placed at sites of high bat activity can reach or exceed the current threshold levels set for large wind turbines. As SWTs provide less energy than large turbines, their negative impact on bats should be minimized by avoidance measures such as a bat-friendly site selection or curtailment algorithms.
Development and validation of a pocket guide for the prevention of diabetic foot ulcers.

OBJECTIVE Diabetic foot ulcers can have serious consequences, including amputation. This project aimed to develop and validate a diabetes care management model-a pocket guide on the prevention of foot ulceration to assist health professionals and scientific societies. METHODS An adaptation of the Iowa method of evidence-based practice to promote high-quality care was employed. After problems are identified, the Iowa method supports the development of an action plan for addressing them. An evidence-based protocol based on the five cornerstones of the 2015 guidance on the diabetic foot by the International Working Group on the Diabetic Foot was developed in two phases and validated using the Delphi technique. RESULTS A model was developed to promote these five cornerstones, which are the main recommendations for managing the diabetic foot. These are: foot examination; risk assessment for ulceration; education in diabetes; appropriate footwear; and treatment of pre-ulcerative lesions. To adapt this into a health information document, the management model was synthesised and designed as a pocket guide. The model's individual and global content validity indices surpass 0.78 and 0.90 respectively. CONCLUSION A management model was created and validated, and produced as a pocket guide to deliver instructions on the care and prevention of diabetic foot problems in people with diabetes.
Risk perceptions and preventive practices of COVID-19 among healthcare professionals in public hospitals in Addis Ababa, Ethiopia

Healthcare professionals are at higher risk of contracting the new coronavirus disease 2019 (COVID-19). Although appropriate preventive measures are the most important interventions to prevent coronavirus infection among healthcare workers, they are also highly concerned about the consequences of the pandemic. The aim of this cross-sectional study was to assess preventive practices, perceived risk and worry about COVID-19 crisis among healthcare professionals at six public hospitals in Addis Ababa, Ethiopia. A systematic random sampling technique was used to select 1,134 respondents (52.6% females). Data were collected between 9th and 20th June 2020 using self-administered questionnaires. Descriptive statistics were used to summarize the data. A multiple linear regression analysis was performed to identify factors associated with worry about COVID-19 crisis. The highest percentage of respondents were nurses (39.3%) and physicians (22.2%), followed by interns (10.8%) and midwives (10.3%). Wearing facemask (93%) and frequent hand washing (93%) were the commonly reported preventive practices. Perceived risk of becoming infected with coronavirus (88%) and the potential risk of infection to their family (91%) were very high. The mean (median) worry score about COVID-19 crisis was 2.37 (3.0), on 1 to 3 scale, with 1 (not worried) and 3 (highly worried). The majority worried a lot about the health system being overwhelmed by COVID-19 patients (92%), the health of their loved ones (90%) and losing someone due to COVID-19 (89%). Respondents who had previously provided clinical care to Ebola, SARS and cholera patients had significantly lower levels of worry about COVID-19 crisis than participants who had not (β = -1.38, P<0.001). Our findings reveal respondents’ widespread practice of preventive measures, highest levels of perceived risk and worry about the COVID-19 crisis. Increased perceived risk and worry about COVID-19 might enable healthcare workers to adopt appropriate preventive measures more effectively against the disease.
T-Cell Receptor Mimic Antibodies for Cancer Immunotherapy

Antibody-based immunotherapies show clinical effectiveness in various cancer types. However, the target repertoire is limited to surface or soluble antigens, which are a relatively small percentage of the cancer proteome. Most proteins of the human proteome are intracellular. Short peptides from intracellular targets can be presented by MHC class I (MHC-I) molecules on cell surface, making them potential targets for cancer immunotherapy. Antibodies can be developed to target these peptide/MHC complexes, similar to the recognition of such complexes by the T-cell receptor (TCR). These antibodies are referred to as T-cell receptor mimic (TCRm) or TCR-like antibodies. Ongoing preclinical and clinical studies will help us understand their mechanisms of action and selection of target epitopes for immunotherapy. The present review will summarize and discuss the selection of intracellular antigens, production of the peptide/MHC complexes, isolation of TCRm antibodies for therapeutic applications, limitations of TCRm antibodies, and possible ways to advance TCRm antibody-based approaches into the clinic.
Intraoperative assessment of canine soft tissue sarcoma by deep learning enhanced optical coherence tomography.

Soft tissue sarcoma (STS) is a locally aggressive and infiltrative tumor in dogs. Surgical resection is the treatment of choice for local tumor control. Currently, post-operative pathology is performed for surgical margin assessment. Spectral-domain optical coherence tomography (OCT) has recently been evaluated for its value for surgical margin assessment in some tumor types in dogs. The purpose of this study was to develop an automatic diagnosis system that can assist clinicians in real-time for OCT image interpretation of tissues at surgical margins. We utilized a ResNet-50 network to classify healthy and cancerous tissues. A patch-based approach was adopted to achieve accurate classification with limited training data (80 cancer images, 80 normal images) and the validation set (20 cancer images, 20 normal images). The proposed method achieved an average accuracy of 97.1% with an excellent sensitivity of 94.3% on the validation set; the quadratic weighted κ was 0.94 for the STS diagnosis. In an independent test data set of 20 OCT images (10 cancer images, 10 normal images), the proposed method correctly differentiated all the STS images. Furthermore, we proposed a diagnostic curve, which could be evaluated in real-time to assist clinicians in detecting the specific location of a lesion. In short, the proposed method is accurate, operates in real-time, and is non-invasive, which could be helpful for future surgical guidance. This article is protected by copyright. All rights reserved.
Should I include studies from "predatory" journals in a systematic review? Interim guidance for systematic reviewers.

ABSTRACT A systematic review involves the identification, evaluation, and synthesis of the best-available evidence to provide an answer to a specific question. The "best-available evidence" is, in many cases, a peer-reviewed scientific article published in an academic journal that details the conduct and results of a scientific study. Any potential threat to the validity of these individual studies (and hence the resultant synthesis) must be evaluated and critiqued.In science, predatory journals continue to rise. Studies published in predatory journals may be of lower quality and more likely to be impacted by fraud and error compared to studies published in traditional journals. This poses a threat to the validity of systematic reviews that include these studies and, therefore, the translation of evidence into guidance for policy and practice. Despite the challenges predatory journals present to systematic reviewers, there is currently little guidance regarding how they should be managed.In 2020, a subgroup of the JBI Scientific Committee was formed to investigate this issue. In this overview paper, we introduce predatory journals to systematic reviewers, outline the problems they present, their potential impact on systematic reviews, and some alternative strategies for consideration of studies from predatory journals in systematic reviews. Options for systematic reviewers could include: excluding all studies from suspected predatory journals, applying additional strategies to forensically examine the results of studies published in suspected predatory journals, stringent search limits, and applying analytical techniques (such as subgroup or sensitivity analyses) to investigate the impact of suspected predatory journals in a synthesis.
Hepatic pathological features in naïve patients with chronic hepatitis C who have developed thyroid disorder

Knowing the hepatic pathological features encountered in patients with chronic hepatitis C (CHC) and the fact that extrahepatic manifestations occur only in people with certain characteristics of the immune system, we tried to evaluate, qualitatively and semi-quantitatively, the liver pathological aspects encountered in 96 patients with CHC, previously untreated with Interferon (naïve), who showed or did not show signs of thyroid disorder (TD), hospitalized in the 2nd Medical Clinic of the Emergency County Hospital, Craiova, Romania, within a period of five years (2007–2012). Following hormonal, immunological, and thyroid ultrasound investigations, 14 (14.58%) of the 96 patients showed signs of TD. The main clinical forms of TD in the studied patients with CHC were autoimmune thyroiditis and subclinical hypothyroidism. In the patients with CHC with TD, we found mild chronic hepatitis in 14.28% of cases, the appearance of moderate chronic hepatitis was found in 71.42% patients, and the appearance of severe chronic hepatitis was found in 14.28% patients, while in the patients with CHC without TD we found chronic mild hepatitis in 62.19% of cases, the appearance of moderate chronic hepatitis was met in 32.92% patients, and the appearance of severe chronic hepatitis was found in 4.87% of patients. Mild and moderate fibrosis were found only in CHC patients without TD in a percentage of 25.6% and 65.85%, respectively, while severe fibrosis was found at 12.19% among CHC patients without TD and 92.85% among CHC patients with TD. The pathological aspect of liver cirrhosis was found only in those with TD (7.14%). In conclusion, the pathological features which define the liver necroinflammatory process, as encountered at the pathological examination in CHC patients with TD are the same as in any active chronic hepatitis, the differences being represented by the higher percentage of the periportal and the preseptal necrosis (piecemeal necrosis), as well as by the higher score of portal inflammation. In addition, the severe hepatic fibrosis and the histopathological appearance of the liver cirrhosis have only defined the cases of CHC with TD.
MODULATION OF APOLIPOPROTEIN B-100 DEGRADATION AND SECRETION BY HEPATIC PROTEIN QUALITY CONTROL COMPONENTS

The central protein component of hepatic very low density lipoproteins (VLDL), apolipoprotein B-100 (apoB-100), is unique in that it is removed via endoplasmic reticulum-associated degradation (ERAD) when poorly lipidated. Protein quality control processes that govern apoB-100 production can impact the secretion rate of VLDL. The human hepatoma cell line HepG2 was used to characterize the functional role of several proteins in the apoB-100 ERAD pathway. A network of cellular protein components serves as the machinery to identify ERAD substrates, remove them from the ER and deliver them to the cytosolic proteasome. Here, the retrotranslocation of apoB-100 out of the ER was found to require p97, as the siRNA-mediated knockdown of p97 impaired the release of apoB-100 and polyubiquitinated apoB into the cytosol. Blocking p97dependent retrotranslocation did not increase apoB-100 secretion, suggesting that apoB100 was committed to degradation by the time it associated with p97. In contrast, knockdown of the ER-resident ubiquitin ligase gp78 decreased apoB-100 ubiquitination and increased apoB-100 secretion. Depletion of gp78 reversed the p97-dependent accumulation of apoB-100, suggesting that gp78 acted upstream of p97 during ERAD. Surprisingly, blocking gp78-dependent ubiquitination of apoB-100 allowed the assembly of more buoyant, lipid-rich VLDL. The cytosolic chaperone “holdase” Bag6 was identified by mass spectrometry and immunoblot to associate with apoB-100 following proteasome inhibition. Bag6 associated with newly synthesized, cytosolic apoB-100. Intriguingly, the depletion of Bag6 protein by siRNA reduced apoB-100 secretion efficiency by as much as 50%. Furthermore, the synthesis of apoB-100 was not affected, suggesting that the loss of Bag6 function increased the degradation of apoB-100. This degradation occured at a post-translational step of assembly by an unidentified mechanism. Taken together, these data suggest that ubiquitination is the committing step in the apoB-100 ERAD pathway and that ubiquitination may play a regulatory role in VLDL assembly. Elements of cellular quality control act as modulators of VLDL output, and may be dysregulated in several human diseases which are characterized by increased VLDL production.
Increasing demand for school counselling through a lay counsellor-delivered classroom sensitisation intervention: a stepped-wedge cluster randomised controlled trial in New Delhi, India

Introduction We evaluated a classroom-based sensitisation intervention that was designed to reduce demand-side barriers affecting referrals to a school counselling programme. The sensitisation intervention was offered in the context of a host trial evaluating a low-intensity problem-solving treatment for common adolescent mental health problems. Methods We conducted a stepped-wedge, cluster randomised controlled trial with 70 classes in 6 secondary schools serving low-income communities in New Delhi, India.The classes were randomised to receive a classroom sensitisation session involving a brief video presentation and moderated group discussion, delivered by a lay counsellor over one class period (intervention condition, IC), in two steps of 4 weeks each. The control condition (CC) was whole-school sensitisation (teacher-meetings and whole-school activities such as poster displays). The primary outcome was the proportion of students referred into the host trial. Secondary outcomes were the proportion of students who met mental health caseness criteria and the proportion of self-referred adolescents. Results Between 20 August 2018 and 9 December 2018, 835 students (23.3% of all students) were referred into the host trial. The referred sample included 591 boys (70.8%), and had a mean age of 15.8 years, SD=0.06; 194 students (31.8% of 610 with complete data) met mental health caseness criteria. The proportion of students referred in each trial conditionwas significantly higher in the IC (IC=21.7%, CC=1.5%, OR=111.36, 95% CI 35.56 to 348.77, p<0.001). The proportion of self-referred participants was also higher in the IC (IC=98.1%, CC=89.1%, Pearson χ2 (1)=16.92, p<0.001). Although the proportion of referred students meeting caseness criteria was similar in both conditions (IC=32.0% vs CC=28.1%), the proportion weighted for the total student population was substantially higher in the IC (IC=5.2%, CC=0.3%, OR=52.39, 95% CI 12.49 to 219.66,p<0.001). Conclusion A single, lay counsellor-delivered, classroom sensitisation session increased psychological help-seeking for common mental health problems among secondary school pupils from urban, low-income communities in India. Trial registration number NCT03633916.
Synthesis, Characterization, and Visible-Light Photo-Fenton Catalytic Activity of Hydroxy Fe/Al-Intercalated Montmorillonite

The photochemically assisted Fenton reaction (photo-Fenton) is important because it may be particularly effective for the degradation of harmful organic compounds in the environment using solar light. The purpose of the present study was to determine the effectiveness of hydroxy Fe/Al-intercalated montmorillonites (Fe/Al-Mt) as photo-Fenton catalysts. In particular, different Fe/Al molar ratios were of interest as a means to vary catalytic activity. Intercalation was achieved via an ion-exchange method and brilliant orange X-GN was the test compound for photodegradation by hydrogen peroxide (H2O2) under visible-light irradiation (γ < 420 nm) in the presence of Fe/Al-Mt. The Fe/Al-Mt materials obtained were characterized by powder X-ray diffraction, N2 adsorption/desorption, X-ray fluorescence spectroscopy, X-ray photoelectron spectroscopic analysis, and ultraviolet-visible spectroscopy. The decoloration performance of Fe/Al-Mt was investigated using different experimental parameters, including the synthesis method, the Fe/Al molar ratio of the intercalating solution, the catalyst dosage, the H2O2 dosage, and the pH. The results of photo-Fenton reaction showed that the photocatalytic activity of Fe/Al-Mt was enhanced significantly by the extent of hydroxy Al/Fe intercalation. For optimal reaction conditions, 99.92% degradation efficiency of X-GN was achieved after 140 min of reaction. To obtain further information on the visible-light-assisted photo-Fenton process, a high-performance liquid chromatography-mass spectrometry method was applied to indentify the intermediate products and a degradation pathway was proposed.
Identification of SLC38A7 as a Prognostic Marker and Potential Therapeutic Target of Lung Squamous Cell Carcinoma

Supplemental Digital Content is available in the text Background: No effective molecular targeted therapy has been established for SCC. We conducted a comprehensive study of SCC patients using RNA-sequencing and TCGA dataset to clarify the driver oncogene of SCC. Method: Forty-six samples of 23 patients were totally analyzed with RNA-sequencing. We then searched for candidate-oncogenes of SCC using the TCGA database. To identify candidate oncogenes, we used the following 2 criteria: (1) the genes of interest were overexpressed in tumor tissues of SCC patients in comparison to normal tissues; and (2) using an integrated mRNA expression and DNA copy number profiling analysis using the TCGA dataset, the DNA copy number of the genes was positively correlated with the mRNA expression. Result: We identified 188 candidate-oncogenes. Among those, the high expression of SLC38A7 was a strong prognostic marker that was significantly associated with a poor prognosis in terms of both overall survival (OS) and recurrence-free survival in the TCGA dataset (P < 0.05). Additionally, 202 resected SCC specimens were also subjected to an immunohistochemical analysis. Patients with the high expression of SLC38A7 (alternative name is sodium-coupled amino acid transporters 7) protein showed significantly shorter OS in comparison to those with the low expression of SLC38A7 protein [median OS 3.9 years (95% confidence interval, 2.4–6.4 years) vs 2.2 years (95% confidence interval, 1.9–4.1 years); log rank test: P = 0.0021]. Conclusion: SLC38A7, which is the primary lysosomal glutamine transporter required for the extracellular protein-dependent growth of cancer cells, was identified as a candidate therapeutic target of SCC.
High concentrations of β‐hydroxybutyrate alter the kinetics of bovine spermatozoa

Postpartum cows, mainly with metabolic diseases, such as ketosis, usually experience an increased number of services per conception. During ketosis, high concentrations of β‐hydroxybutyrate (BHBA) in follicular, uterine and oviductal fluid have been considered to cause subfertility in cows. However, the effect of sperm exposure to an environment with high BHBA concentration is not known. This study investigated the influence of high levels of BHBA on kinetics, oxidative status and morphology of bovine spermatozoa. To assess the effect of BHBA after sperm selection, bovine spermatozoa were incubated (180 min) with different BHBA concentrations: 0 (Control), 0.8, 2.4 or 5 mM. Sperm kinetics was evaluated after 30, 60, 120 and 180 min, and oxidative status and morphology were analysed at 180 min. Oxidative status was evaluated through the production of reactive oxidative species (ROS), total antioxidant capacity and lipid peroxidation. High concentrations of BHBA decreased the curvilinear velocity, straight line velocity, mean path velocity, linearity, straightness and hyperactivity of spermatozoa. However, there was no effect of BHBA on oxidative and antioxidant capacity as well as on sperm morphology. In conclusion, exposure of bovine spermatozoa to high levels of BHBA impairs sperm kinetics without altering oxidative and antioxidant mechanisms.