Miracle-dz/UniFAR-FTUs huggingface dataset:
Time-course Comparison of Xenobiotic Activators of CAR and PPARα in Mouse Liver

Constitutive androstane receptor (CAR) and peroxisome proliferator activated receptor (PPAR)α are transcription factors known to be primary mediators of liver effects, including carcinogenesis, by phenobarbital-like compounds and peroxisome proliferators, respectively, in rodents. Many similarities exist in the phenotypes elicited by these two classes of agents in rodent liver, and we hypothesized that the initial transcriptional responses to the xenobiotic activators of CAR and PPARα will exhibit distinct patterns, but at later time-points these biological pathways will converge. In order to capture the global transcriptional changes that result from activation of these nuclear receptors over a time course in the mouse liver, microarray technology was used. First, differences in basal expression of liver genes between C57Bl/6J wild-type and Car-null mice were examined and 14 significantly differentially expressed genes were identified. Next, mice were treated with phenobarbital (100 mg/kg by gavage for 24 hrs, or 0.085% w/w diet for 7 or 28 days), and liver gene expression changes with regards to both time and treatment were identified. While several pathways related to cellular proliferation and metabolism were affected by phenobarbital in wild-type mice, no significant changes in gene expression were found over time in the Car-nulls. Next, we determined commonalities and differences in the temporal response to phenobarbital and WY-14,643, a prototypical activator of PPARα. Gene expression signatures from livers of wild-type mice C57Bl6/ J mice treated with PB or WY-14,643 were compared. Similar pathways were affected by both compounds; however, considerable time-related differences were present. This study establishes common gene expression fingerprints of exposure to activators of CAR and PPARα in rodent liver and demonstrates that despite similar phenotypic changes, molecular pathways differ between classes of chemical carcinogens.
This information is current as Mononuclear Cells Normal Human Peripheral Blood Myeloid-Derived Suppressor Cells from Characterization of Cytokine-Induced

Tumor immune tolerance can derive from the recruitment of suppressor cell populations, including myeloid-derived suppressor cells (MDSCs). In cancer patients, increased MDSCs correlate with more aggressive disease and a poor prognosis. Expression of 15 immune factors (TGF b , IL-1 b , IL-4, IL-6, IL-10, GM-CSF, M-CSF, IDO, fms -related tyrosine kinase 3 ligand, c- kit ligand, inducible NO synthase, arginase-1, TNF- a , cyclo-oxygenase 2, vascular endothelial growth factor [VEGF]) by MDSC-inducing human solid tumor cell lines was evaluated by RT-PCR. Based upon these data, cytokine mixtures were then tested for their ability to generate suppressive CD33 + cells from healthy donor PBMCs in vitro by measuring their ability to inhibit the proliferation of, and IFN- g production by, fresh autologous human T cells after CD3/CD28 stimulation. Induced MDSCs were characterized with respect to their morphology, surface phenotype, and gene expression proﬁle. MDSC-inducing cancer cell lines demonstrated multiple pathways for MDSC generation, including overexpression of IL-6, IL-1 b , cyclo-oxygenase 2, M-CSF, and IDO. CD33 + cells with potent suppressive capacity were best generated in vitro by GM-CSF and IL-6, and secondarily by GM-CSF + IL-1 b , PGE 2 , TNF- a , or VEGF. Characterization studies of cytokine-induced suppressive cells revealed CD33 + CD11b + CD66b + HLA-DR low IL-13R a 2 int large mononuclear cells with abundant basophilic cytoplasm. Expression of inducible NO synthase, TGF b , NADPH oxidase, VEGF, and/or arginase-1 was also upregulated, and Transwell studies showed suppression of autologous T cells to be contact dependent. Suppressive CD33 + cells generated from PBMCs by GM-CSF and IL-6 were consistent with human MDSCs. This study suggests that these cytokines are potential therapeutic targets for the inhibition of MDSC induction in cancer patients. The Journal of Immunology , 2010, 185: 2273–2284. CD33 + MDSCs generated in the absence of T cells demonstrated a comparable suppressive capacity to those generated from whole PBMCs for most cytokine mixtures examined. However, for cytokine mixtures GM-CSF alone, GM-CSF + TNF- a , and GM-CSF + VEGF, CD33 + generated in the absence of T cells were more suppressive than those generated from whole PBMCs ( p , 0.05). C and D , To shed light on possible sources of GM-CSF for the induction of suppressor cells in tumor–PBMC cocultures, gene expression of GM-CSF in PBMCs following direct coculture with inducing tumor cell lines was measured by qRT-PCR techniques and compared with cells cultured in medium alone (mean shown + SD). GM-CSF expression by PBMCs was strongly upregulated following tumor cell line coculture. Analysis of GM-CSF expression in the CD33 + cell fraction suggests that the increase in GM-CSF observed is not localized to this subpopulation.
DEOXYRIBOZYMES IN DETECTION OF PATHOGENIC BACTERIA

The emergence of pathogen outbreaks imposes a significant threat to humankind and is responsible for global deaths, hospitalizations, and economic down growth. Therefore, early detection and prevention of these outbreaks areessential. Detecting and preventing pathogen outbreaks at the earliest possible stage requires highly sensitive, specific, and rapid analytical methods. Conventional detection methods such as cell cultures, enzyme-linked immunosorbent assay (ELISA) [1], and polymerase chain reactions (PCR) [2] are available to detect pathogens. Still, these methods are associated with drawbacks such as high time consumption, the requirement of expert knowledge, and highly sophisticated laboratories. Furthermore, in the case of severe pathogen outbreaks, these techniques cannot be used to detect and prevent the spreading since the detection process requires a longer time to analyze the samples [3]. PCR-based detection methods are capable of detecting the pathogen bacteria in less time compared to the other methods. But it requires multiple sample preparation steps such as extraction, isolations, and purification of the bacterial DNA samples. The use of thermocycler for the PCR amplification makes it inconvenient to use it effectively. The detection of pathogen bacteria by ELISA is based on the specific antigen-antibody interactions and it provides a wide range of pathogen bacteria detection. Even though, ELISA contains drawbacks such as expensiveness in synthesizing antibodies and less sensitivity due to the cross contaminations. Therefore, compared to the conventional detection methods, effective analytical methods are required to detect and UDC 576.522; 576.523; 576.385. 5; 571.27; 616-006 https://doi.org/10.15407/biotech14.05. 005
NDT_A_266136 1929..1941

1Department of Neurotrauma, Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, People’s Republic of China; 2Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, People’s Republic of China; 3Department of Acupuncture, Lianyungang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Lianyungang City 222000, Jiangsu Province, People’s Republic of China; 4Department of Neuropharmacology, Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Capital Medical University, Beijing 100070, People’s Republic of China
EFFECT OF CERTAIN DIETARY VITAMINS SUPPLEMENTATION ON SOME PRODUCTIVE, REPRODUCTIVE AND HATCHING PERFORMANCE OF JAPANESE QUAIL UNDER EGYPTIAN SUMMER CONDITIONS

This work was conducted to study the effect of dietary supplementation of vitamins A, E, C, BR6R and BR12R on some productive and reproductive traits of mature Japanese quails during hot summer season under Egyptian environmental conditions. A total number of 180 Japanese quail breeders (126 females and 54 males), 8 weeks old with nearly equal body weight (215 to 250 g) were randomly divided into six treatment groups (21 females and 9 males in each group). Birds in the first experimental group received the basal diet (without supplementation) used as control, while birds in 2 PP, 3PP, 4PP, 5P Pand 6 PP groups, were received the basal diet supplemented with one of the following vitamins, A (5 mg /kg diet), E (150 mg /kg diet), C (250 mg /kg diet), BR6R (3 mg /kg diet) or BR12R (2 mg /kg diet), respectively. Egg production, egg quality, semen characteristics as well as hatchability (%) and hatched chick performance were determined. Results indicated that the addition of vitamin C or BR6R in the diets of Japanese quail layers, significantly (P ≤ 0.01) improved egg number and feed conversion ratio (FCR) comparatively with those unsupplemented treatments, while egg mass values were improved significantly (P ≤ 0.05) in layers fed diet supplemented with vitamin E, C, or BR6R when compared with layers fed basal diet. The highest egg length value was obtained for hens fed diets supplemented with 150 mg vitam. E /kg diet, while the lowest value was obtained with those fed diets supplemented with 3 mg vitam. BR6R /kg diet when compared with control. Birds fed diet supplemented with 150 mg vitam. E/kg diet recorded the lowest value of egg shape index comparatively with the control and other treatment groups. Japanese quail layers given BR6R or BR12R had significantly (P ≤ 0.01) higher hatchablility values than those unsupplemented with vitamins. Semen characteristics studied [mass motility (%), individual motility (%) and dead sperm (%)] were significantly (P ≤ 0.01) improved male Japanese quail fed diets supplemented with A, E, C and BR6R in comparison with these supplemented with vitam. BR12 Rand the control group. It could be concluded that supplementing quail breeder diet with vitamins BR6R, E and C, improved the productive performance, while vitam. BR6R gave the higher hatchability percentage. The best semen characteristics were obtained by vitam. E supplementation.
MEASUREMENTS IN SEPARATED AND TRANSITIONAL BOUNDARY LAYERS UNDER LOW-PRESSURE TURBINE AIRFOIL CONDITIONS

Detailed velocity measurements were made along a flat plate subject to the same dimensionless pressure gradient as the suction side of a modern low-pressure turbine airfoil. Reynolds numbers based on wetted plate length and nominal exit velocity were varied from 50,000 to 300,000, covering cruise to takeoff conditions. Low and high inlet free-stream turbulence intensities (0.2% and 7%) were set using passive grids. The location of boundary-layer separation does not depend strongly on the free-stream turbulence level or Reynolds number, as long as the boundary layer remains non-turbulent prior to separation. Strong acceleration prevents transition on the upstream part of the plate in all cases. Both free-stream turbulence and Reynolds number have strong effects on transition in the adverse pressure gradient region. Under low free-stream turbulence conditions transition is induced by instability waves in the shear layer of the separation bubble. Reattachment generally occurs at the transition start. At Re = 50, 000 the separation bubble does not close before the trailing edge of the modeled airfoil. At higher Re, transition moves upstream, and the boundary layer reattaches. With high free-stream turbulence levels, transition appears to occur in a bypass mode, similar to that in attached boundary layers. Transition moves upstream, resulting in shorter separation regions. At Re above 200, 000, transition begins before separation. Mean velocity, turbulence and intermittency profiles are presented.
Machine and Deep Learning-based Beam Selection for Hybrid Beamforming with Partial CSI

—This paper investigates the applicability of deep and machine learning techniques to perform beam selection in the uplink of a mmWave communication system. Speciﬁcally, we consider a hybrid beamforming setup comprising an analog beamforming (ABF) network followed by a zero-forcing baseband processing block. The goal is to select the optimal conﬁguration for the ABF network based on partial Channel State Information (CSI), namely, the estimated angles-of-arrival (AoAs) and the received power of the various users. To that aim, we consider (i) two supervised machine-learning approaches: k-nearest neighbors (kNN) and support vector classiﬁers (SVC); and (ii) a feed-forward deep neural network: the multilayer perceptron (MLP). Computer simulations reveal that, for a speciﬁcally-designed codebook of analog beamformers, this task can be effectively accomplished by such data-driven schemes. Performance, in terms of classiﬁcation accuracy and sum-rate, is very close to that achievable via exhaustive search.
for cervical

diagnosed in adults, and 4 cases (6%) of TB were diagnosed in children. (cid:36)(cid:80)(cid:79)(cid:68)(cid:77)(cid:86)(cid:84)(cid:74)(cid:80)(cid:79) Our study shows more malignancy and much less TB than a prior study of cervical lymphadenopathy at KCH. With the successful initiaion of the KCH Pathology Laboratory in 2011, we recommend biopsy or FNA early in the workup of cervical lymphadenopathy to prevent long delays in diagnosis and treatment of curable cancers.
Methodology for measuring oxidative capacity of isolated peroxisomes in the Seahorse assay

The regulation of cellular energetics is a complex process that requires the coordinated function of multiple organelles. Historically, studies focused on understanding cellular energy utilization and production have been overwhelmingly concentrated on the mitochondria. While mitochondria account for the majority of intracellular energy production, they alone are incapable of maintaining the variable energetic demands of the cell. The peroxisome has recently emerged as a secondary metabolic organelle that complements and improves mitochondrial performance. Although mitochondria and peroxisomes are structurally distinct organelles, they share key functional similarities that allows for the potential to repurpose readily available tools initially developed for mitochondrial assessment to interrogate peroxisomal metabolic function in a novel manner. To this end, we report here on procedures for the isolation, purification and real-time metabolic assessment of peroxisomal β-oxidation using the Agilent Seahorse® system. When used together, these protocols provide a straightforward, reproducible and highly quantifiable method for measuring the contributions of peroxisomes to cellular and organismal metabolism.
International Conference on Psychology and Education THE DARK TRIAD OF PERSONALITY AND WORK EFFICIENCY OF KINDERGARTEN TEACHERS

The current study investigates the association between work efficiency indicators and the Dark Triad of personality traits (Machiavellianism, narcissism, and psychopathy) within the professional group of kindergarten teachers. The sample included 129 female Russian kindergarten teachers who completed a Russian-language adaptation of the Short Dark Triad questionnaire (SD3). The work efficiency included 5 indicators evaluated by experts and the total efficiency score (the average of the five indicators). The results obtained with one-way ANOVA showed significant (p < .05) association between the Dark Triad traits and some work efficiency indicators. The specificity of these associations for different traits of the Dark Triad was found to be manifested in two aspects: (1) sets of vulnerable efficiency indicators, and (2) the type of association in relation to the level of a trait. Thus, high Machiavellianism was associated with lower scores for the efficiency indicator "Professional competence", whereas both high and low psychopathy were associated with reduced scores for the indicator "Functional completeness". Finally, unlike both of these cases, narcissism had an ambiguous effect: all four efficiency indicators were sensitive to its effects (functional completeness, labor creativity, professional motivation, total efficiency), decreasing with both low and high narcissism. This result is consistent with an unfolding discussion about the special role of narcissism within the Dark Triad. The results of the study suggest certain requirements to a kindergarten teacher’s personality, namely low or medium Machiavellianism, medium narcissism and low psychopathy. © 2017 Published by Future Academy www.FutureAcademy. org.UK
ShopMiner: Mining customer shopping behavior in physical clothing stores with passive RFIDs

Shopping behavior data are of great importance to understand the effectiveness of marketing and merchandising efforts. Online clothing stores are capable capturing customer shopping behavior by analyzing the click stream and customer shopping carts. Retailers with physical clothing stores, however, still lack effective methods to identify comprehensive shopping behaviors. In this paper, we show that backscatter signals of passive RFID tags can be exploited to detect and record how customers browse stores, which items of clothes they pay attention to, and which items of clothes they usually match with. The intuition is that the phase readings of tags attached on desired items will demonstrate distinct yet stable patterns in the time-series when customers look at, pick up or turn over desired items. We design ShopMiner, a framework that harnesses these unique spatial-temporal correlations of time-series phase readings to detect comprehensive shopping behaviors. We have implemented a prototype of ShopMiner with a COTS RFID reader and four antennas, and tested its effectiveness in two typical indoor environments. Empirical studies from twoweek shopping-like data show that ShopMiner could achieve high accuracy and efficiency in customer shopping behavior identification.
Clustered approach to Web Search Using SVM as Load Balancing Module

: The rapid growth of the Internet has made the Web a popular place for collecting information. Today, Internet user access billions of web pages online using search engines. Information in the Web comes from many sources, including websites of companies, organizations, communications etc. Effective representation of Web search results remains an open problem in the Information Retrieval community. To overcome this, the relevant Web pages are often located close to each other in the Web graph of hyperlinks. It presents a graphical approach for entity resolution & complements the traditional methodology with the analysis of the entity-relationship (ER) graph constructed for the dataset being analyzed. It can significantly improve the quality of entity resolution. Using Support Vector Machines (SVMs) as supervised learning methods distributes the workload over the network by assigning the capacity to handle the number of requests at a time. Hence provide the stable system with quality results.
EAAE 2008 Congress– Market Driven Entrepreneurship: The Convergence of Market Orientation and the Resource Based View

The dramatically changing marketing structure for agricultural products is forcing agricultural producers to become more entrepreneurial, but what does this mean? How do agricultural producers produce sustainable competitive advantage within a commodity market characterised by homogeneous producers? The convergence of two academic literatures Market orientation from market strategy and Resource Based View from strategic management – provide a number of insights. In this paper we lay the foundation for research into the market orientation–performance link in terms of production agriculture. Building on the previous market orientation literature, we examine its ability to create a sustainable competitive advantage using a resource based view (RBV). Focusing primarily on the U.S. beef industry, we examine these two distinct literatures in tandem and show that the concept of a market orientation meets all the necessary conditions laid out in the RBV literature to create a sustainable competitive advantage, even in a commodity marketplace such as agriculture. This convergence between these two literatures has important implications and direction for future research as we attempt to analyze and evaluate the heterogeneous responses of firm the new global agri-food business environment. Keywords—Market Orientation, Resource Based View, Production Agriculture
Response of littoral chironomid community and organic matter to late glacial lake level and environmental changes at Lago dell'Accesa (Tuscany, Italy)

This study focuses on the response of lacustrine littoral chironomid communities to late glacial changes in limnological, environmental and climate conditions in the Mediterranean context. Late glacial chironomid (Diptera: Chironomidae) assemblages, organic petrography and geochemistry were analysed in a sediment core from the littoral zone of Lago dell’Accesa (Tuscany, Italy), where the lake-level fluctuations and the vegetation history have been previously reconstructed. Comparison of the chironomid stratigraphy to other proxies (pollen assemblages, organic petrography and geochemistry, lake-level) and regional climate reconstruction suggested the predominant influence of lake-level changes on the littoral chironomid fauna. The main lowering events that occurred during the Oldest and the Younger Dryas were followed by higher proportions of taxa typical of littoral habitats. A complementary study of organic matter suggested the indirect impact of lake-level on the chironomids through changes in humic status and habitat characteristics, such as the type of substrate and aquatic macrophyte development. Several chironomid taxa, such as Glyptotendipes, Microtendipes and Cricotopus type patens, were identified as possible indicators of low lake-level in the late glacial records. Nevertheless, this study suggested that parallel analyses of organic matter and chironomid assemblages may be needed to circumvent misinterpretation of littoral chironomid assemblage stratigraphy. There was a weak response of the chironomid assemblages to small lake-level lowerings that corresponded to the Older Dryas and Preboreal oscillations. A higher level of determination, e.g. to the species group level, may be necessary to increase the sensibility of the indicators to lake-level changes.
The Ubiquitin-Speciﬁc Protease Subfamily UBP3/UBP4 Is Essential for Pollen Development and Transmission in Arabidopsis 1[W][OA]

Deubiquitinating enzymes are essential to the ubiquitin (Ub)/26S proteasome system where they release Ub monomers from the primary translation products of poly-Ub and Ub extension genes, recycle Ubs from polyubiquitinated proteins, and reverse the effects of ubiquitination by releasing bound Ubs from individual targets. The Ub-speciﬁc proteases (UBPs) are one large family of deubiquitinating enzymes that bear signature cysteine and histidine motifs. Here, we genetically characterize a UBP subfamily in Arabidopsis ( Arabidopsis thaliana ) encoded by paralogous UBP3 and UBP4 genes. Whereas homozygous ubp3 and ubp4 single mutants do not display obvious phenotypic abnormalities, double-homozygous mutant individuals could not be created due to a defect in pollen development and/or transmission. This pollen defect was rescued with a transgene encoding wild-type UBP3 or UBP4, but not with a transgene encoding an active-site mutant of UBP3, indicating that deubiquitination activity of UBP3/UBP4 is required. Nuclear DNA staining revealed that ubp3 ubp4 pollen often fail to undergo mitosis II, which generates the two sperm cells needed for double fertilization. Substantial changes in vacuolar morphology were also evident in mutant grains at the time of pollen dehiscence, suggesting defects in vacuole and endomembrane organization. Even though some ubp3 ubp4 pollen could germinate in vitro, they failed to fertilize wild-type ovules even in the absence of competing wild-type pollen. These studies provide additional evidence that the Ub/26S proteasome system is important for male gameto- genesis in plants and suggest that deubiquitination of one or more targets by UBP3/UBP4 is critical for the development of functional pollen.