Miracle-dz/UniFAR-FTUs huggingface dataset:
PPRV-Induced Autophagy Facilitates Infectious Virus Transmission by the Exosomal Pathway

Autophagy plays an important role in PPRV pathogenesis. The role of exosomes in viral infections is beginning to be appreciated. ABSTRACT Peste des petits ruminants virus (PPRV) is an important pathogen that seriously influences the productivity of small ruminants worldwide. We showed previously that PPRV induced sustained autophagy for their replication in host cells. Many studies have shown that exosomes released from virus-infected cells contain a variety of viral and host cellular factors that are able to modulate the recipient’s cellular response and result in productive infection of the recipient host. Here, we show that PPRV infection results in packaging of the viral genomic RNA and partial viral proteins into exosomes of Vero cells and upregulates exosome secretion. We provide evidence showing that the exosomal viral cargo can be transferred to and establish productive infection in a new target cell. Importantly, our study reveals that PPRV-induced autophagy enhances exosome secretion and exosome-mediated virus transmission. Additionally, our data show that TSG101 may be involved in the sorting of the infectious PPRV RNA into exosomes to facilitate the release of PPRV through the exosomal pathway. Taken together, our results suggest a novel mechanism involving autophagy and exosome-mediated PPRV intercellular transmission. IMPORTANCE Autophagy plays an important role in PPRV pathogenesis. The role of exosomes in viral infections is beginning to be appreciated. The present study examined the role of autophagy in secretion of infectious PPRV from Vero cells. Our data provided the first direct evidence that ATG7-mediated autophagy enhances exosome secretion and exosome-mediated PPRV transmission. TSG101 may be involved in the sorting of the infectious PPRV RNA genomes into exosomes to facilitate the release of PPRV through the exosomal pathway. Inhibition of PPRV-induced autophagy or TSG101 expression could be used as a strategy to block exosome-mediated virus transmission.
Comment on "Soil CO 2 , CH 4 and N 2 O fluxes from an afforested lowland raised peat bog in Scotland: implications for drainage and restoration" by Yamulki et al. (2013)

Yamulki and co-authors address in their recent publication the important issue of net emissions of greenhouse gases (GHGs) from peatlands where land use conversion has taken place. In their case, they studied conversion to forestry versus peatland restoration after a first rotation of plantation forestry. They monitored soil-derived fluxes of carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O) using opaque chamber measurements on planted and unplanted control treatments (with or without drainage), and an unplanted plot within a restored (felled) block on former lowland raised bog. They propose that their measurements of greenhouse gas (GHG) emissions at these sites suggest that the total net GHG emissions, in 100 yr carbon dioxide equivalents, of the restored peat bog would be higher than that of the peat bog with trees. We believe there are a number of issues with the measurement, calculation and comparison of these greenhouse budgets that may invalidate this conclusion.
Mechanistic study on the ferric chloride-based rapid cultivation and enhancement of aerobic granular sludge

ABSTRACT Aerobic granular sludge (AGS) can achieve simultaneous carbon, nitrogen and phosphorus removal owing to its three-dimensional oxygen gradient structure. However, long start-up period and poor operational stability restrict its application and promotion. A novel rapid granulation strategy, viz., the short-term (7 days) addition of ferric chloride at the commissioning stage, was developed and verified in this study. The granulation period was shortened by 9 days, and the formed granules were compact and dense with an Fe3+ concentration of 250 mg L−1. The addition of flocculant not only maintained a high sludge concentration during the initial stages of granulation (5.3 g L−1), but also stimulated the secretion of TB-EPS and increased protein and polysaccharide contents, thereby expediting granule formation. Additionally, ferric chloride induced a diverse microbial community in granules, resulting in the emergence of new genera, such as Thaurea, Brevundimonas and Kinneretia, which improved pollutant removal performance and flocculent aggregation. The removal efficiencies of COD, PO4 3--P, and NH4 +-N stabilized at 94.2, 62.4, and 71.3%, respectively. Therefore, it has been demonstrated that short-term ferric chloride dosing has a synergistic effect on aerobic granulation. GRAPHICAL ABSTRACT
Near-Complete Proviral Genome Sequence of Reticuloendotheliosis Virus Isolated from an Attwater’s Prairie Chicken (Tympanuchus cupido attwateri)

We report the near-complete proviral genome sequence of a reticuloendotheliosis virus isolated and propagated from an endangered Attwater’s prairie chicken (Tympanuchus cupido attwateri) during a 2016–2017 outbreak at a captive breeding facility. ABSTRACT We report the near-complete proviral genome sequence of a reticuloendotheliosis virus isolated and propagated from an endangered Attwater’s prairie chicken (Tympanuchus cupido attwateri) during a 2016–2017 outbreak at a captive breeding facility.
COVID-19 Mitigation Among College Students: Social Influences, Behavioral Spillover, and Antibody Results

ABSTRACT By fall 2020, students returning to U.S. university campuses were mandated to engage in COVID-19 mitigation behaviors, including masking, which was a relatively novel prevention behavior in the U.S. Masking became a target of university mandates and campaigns, and it became politicized. Critical questions are whether the influences of injunctive norms and response efficacy on one behavior (i.e. masking) spill over to other mitigation behaviors (e.g. hand-washing), and how patterns of mitigation behaviors are associated with clinical outcomes. We conducted a cross-sectional survey of college students who returned to campus (N = 837) to explore these questions, and conducted COVID-19 antibody testing on a subset of participants to identify correlations between behaviors and disease burden. The results showed that college students were more likely to intend to wear face masks as they experienced more positive injunctive norms, liberal political views, stronger response efficacy for masks, and less pessimism. Latent class analysis revealed four mitigation classes: Adherents who intended to wear face masks and engage in the other COVID-19 mitigation behaviors; Hygiene Stewards and Masked Symptom Managers who intended to wear masks but only some other behaviors, and Refusers who intended to engage in no mitigation behaviors. Importantly, the Hygiene Stewards and Refusers had the highest likelihood of positive antibodies; these two classes differed in their masking intentions, but shared very low likelihoods of physical distancing from others and avoiding crowds or mass gatherings. The implications for theories of normative influences on novel behaviors, spillover effects, and future messaging are discussed.
Electronic and optical properties of Janus black arsenic-phosphorus AsP quantum dots under magnetic field

By utilizing the tight-binding method, the electronic spectrum and states distribution of square Janus monolayer black arsenic phosphorus (b-AsP) quantum dots (QDs) in the presence of a perpendicular magnetic field are explored. Strong in-gap states of b-AsP QDs, whose probability densities are distributed on the armchair boundary (armchair edge states) appear in the energy gap of host perfect two-dimensional b-AsP. The corresponding energy levels of the armchair edge states can degenerate to the Landu energy levels upon applying a perpendicular magnetic field. When an in-plane polarized light is introduced, due to the presence of armchair edge states, the edge-to-edge transitions are mainly induced from the armchair edge (hole) states to zigzag edge (electron) states. The optical absorption undergoes blue shift as a function of the magnetic field. Our work suggests tunable optical properties via modulating the armchair edge states of a b-AsP QD and provides a theoretical basis for the design of b-AsP-based optoelectronic devices.
Reverse Genetics System for Heartland Bandavirus: NSs Protein Contributes to Heartland Bandavirus Virulence

Heartland bandavirus (HRTV) is a tick-borne virus identified in the United States in 2009. HRTV causes fever, fatigue, decreased appetite, headache, nausea, diarrhea, and muscle or joint pain in humans. ABSTRACT Heartland bandavirus (HRTV), which is an emerging tick-borne virus first identified in Missouri in 2009, causes fever, fatigue, decreased appetite, headache, nausea, diarrhea, and muscle or joint pain in humans. HRTV is genetically close to Dabie bandavirus, which is the causative agent of severe fever with thrombocytopenia syndrome (SFTS) in humans and is known as SFTS virus (SFTSV). The generation of infectious HRTV entirely from cloned cDNAs has not yet been reported. The absence of a reverse genetics system for HRTV has delayed efforts to understand its pathogenesis and to generate vaccines and antiviral drugs. Here, we developed a reverse genetics system for HRTV, which employs an RNA polymerase I-mediated expression system. A recombinant nonstructural protein (NSs)-knockout HRTV (rHRTV-NSsKO) was generated. We found that NSs interrupted signaling associated with innate immunity in HRTV-infected cells. The rHRTV-NSsKO was highly attenuated, indicated by the apparent absence of symptoms in a mouse model of HRTV infection. Moreover, mice immunized with rHRTV-NSsKO survived a lethal dose of HRTV. These findings suggest that NSs is a virulence factor of HRTV and that rHRTV-NSsKO could be a vaccine candidate for HRTV. IMPORTANCE Heartland bandavirus (HRTV) is a tick-borne virus identified in the United States in 2009. HRTV causes fever, fatigue, decreased appetite, headache, nausea, diarrhea, and muscle or joint pain in humans. FDA-approved vaccines and antiviral drugs are unavailable. The lack of a reverse genetics system hampers efforts to develop such antiviral therapeutics. Here, we developed a reverse genetics system for HRTV that led to the generation of a recombinant nonstructural protein (NSs)-knockout HRTV (rHRTV-NSsKO). We found that NSs interrupted signaling associated with innate immunity in HRTV-infected cells. Furthermore, rHRTV-NSsKO was highly attenuated and immunogenic in a mouse model. These findings suggest that NSs is a virulence factor of HRTV and that rHRTV-NSsKO could be a vaccine candidate for HRTV.
IconIntent: Automatic Identification of Sensitive UI Widgets Based on Icon Classification for Android Apps

Many mobile applications (i.e., apps) include UI widgets to use or collect users' sensitive data. Thus, to identify suspicious sensitive data usage such as UI-permission mismatch, it is crucial to understand the intentions of UI widgets. However, many UI widgets leverage icons of specific shapes (object icons) and icons embedded with text (text icons) to express their intentions, posing challenges for existing detection techniques that analyze only textual data to identify sensitive UI widgets. In this work, we propose a novel app analysis framework, ICONINTENT, that synergistically combines program analysis and icon classification to identify sensitive UI widgets in Android apps. ICONINTENT automatically associates UI widgets and icons via static analysis on app's UI layout files and code, and then adapts computer vision techniques to classify the associated icons into eight categories of sensitive data. Our evaluations of ICONINTENT on 150 apps from Google Play show that ICONINTENT can detect 248 sensitive UI widgets in 97 apps, achieving a precision of 82.4%. When combined with SUPOR, the state-of-the-art sensitive UI widget identification technique based on text analysis, SUPOR +ICONINTENT can detect 487 sensitive UI widgets (101.2% improvement over SUPOR only), and reduces suspicious permissions to be inspected by 50.7% (129.4% improvement over SUPOR only).
High Performance Vector Control of 3-Phase IM Drives under Open-Phase Fault Based on EKF for Rotor Flux Estimation

This paper proposes a novel flux observer based on Extended Kalman Filter (EKF) for high performance vector control of 3-phase Induction Motor (IM) drives under stator winding open-phase fault. The presented flux estimation combines the Indirect Rotor Field-Oriented Control (IRFOC) method. The rotor flux is obtained from two modified EKF with two different stator currents (forward and backward stator currents). The proposed technique can significantly reduce the DC-offset problem on the pure integrator associated with the basic IRFOC method. The Matlab simulation results confirm the validity of the proposed strategy.
Gender bias in the evaluation of interns in different medical specialties: An archival study

Abstract Introduction The field of medicine is characterized by within-field gender segregation: Gender ratios vary systematically by subdisciplines. This segregation might be, in part, due to gender bias in the assessment of women and men medical doctors. Methods We examined whether the assessments, i.e. overall score, department scores and skills scores, interns receive by their superiors during their internship year, vary as a function of their gender and the representation of women in the field. We analyzed an archival data set from a large hospital in Israel which included 3326 assessments that were given to all interns who completed their internship year between 2015 and 2019. Results Women received lower department scores and skills scores in fields with a low (versus high) representation of women. Men received higher scores in fields with a high (versus low) representation of men, yet there was no difference in their skills scores. Conclusions Women are evaluated more negatively in fields with a low representation of women doctors. Similarly, men are evaluated more negatively in fields with a low representation of men, yet this cannot be explained by their skills. This pattern of results might point to a gender bias in assessments. A better understanding of these differences is important as assessments affect interns’ career choices and options.
Development of an interactive anonymous real-time formative feedback system

This paper is focusing IT-supported real-time formative feedback in a classroom context. The development of a Student and Teacher Response System (STRS) is described. Since there are a number of obstacles for effective interaction in large classes IT can be used to support the teachers aim to find out if students understand the lecture and accordingly adjust the content and design of the lecture. The system can be used for formative assessment before, during, and after a lecture. It is also possible for students to initiate interaction during lectures by posing questions anonymously. The main contributions of the paper are a) the description of the interactive real-time system and b) the development process
ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peripartum cardiomyopathy in mice

Pregnancy is associated with substantial physiological changes of the heart, and disruptions in these processes can lead to peripartum cardiomyopathy (PPCM). The molecular processes that cause physiological and pathological changes in the heart during pregnancy are not well characterized. Here, we show that mTORc1 was activated in pregnancy to facilitate cardiac enlargement that was reversed after delivery in mice. mTORc1 activation in pregnancy was negatively regulated by the mRNA-destabilizing protein ZFP36L2 through its degradation of Mdm2 mRNA and P53 stabilization, leading to increased SESN2 and REDD1 expression. This pathway impeded uncontrolled cardiomyocyte hypertrophy during pregnancy, and mice with cardiac-specific Zfp36l2 deletion developed rapid cardiac dysfunction after delivery, while prenatal treatment of these mice with rapamycin improved postpartum cardiac function. Collectively, these data provide what we believe to be a novel pathway for the regulation of mTORc1 through mRNA stabilization of a P53 ubiquitin ligase. This pathway was critical for normal cardiac growth during pregnancy, and its reduction led to PPCM-like adverse remodeling in mice.
Dust temperature and mid-to-total infrared color distributions for star-forming galaxies at 0

We present a new, publicly available library of dust spectral energy distributions (SEDs). These SEDs are characterized by only three parameters: the dust mass (Mdust), the dust temperature (Tdust), and the mid-to-total infrared color (IR8=LIR/L8). The latter measures the relative contribution of PAH molecules to the LIR. We used this library to model star-forming galaxies at 0.5 1, 3.2, 3.8, and 5.7, respectively. Starburst galaxies above the MS have LIR significantly underestimated and Mdust overestimated. These results can be used immediately to interpret more accurately the large amount of archival data from Spitzer, Herschel and ALMA. [abridged]
1 IMPLICIT AND EXPLICIT STANCES IN LOGIC

We identify a pervasive contrast between implicit and explicit stances in logical analysis and system design. Implicit systems change received meanings of logical constants and sometimes also the notion of consequence, while explicit systems conservatively extend classical systems with new vocabulary. We illustrate the contrast for intuitionistic and epistemic logic, then take it further to information dynamics, default reasoning, and other areas, to show its wide scope. This gives a working understanding of the contrast, though we stop short of a formal definition, and acknowledge limitations and borderline cases. Throughout we show how awareness of the two stances suggests new logical systems and new issues about translations between implicit and explicit systems, linking up with foundational concerns about identity of logical systems. But we also show how a practical facility with these complementary working styles has philosophical consequences, as it throws doubt on strong philosophical claims made by just taking one design stance and ignoring alternative ones. We will illustrate the latter benefit for the case of logical pluralism and hyper-intensional semantics.
The Impact of a Rural Road Development Project on Multidimensional Poverty in Nepal

Although the effect of rural road development projects on income poverty has been well studied, little research has been undertaken on the impact on the multiple dimensions of poverty. In this study, we examine the effect of the improvement and construction of rural roads in rural Nepal on household deprivation of health, education, and living standards. We use data from two rounds of the Nepal Demographic and Health Survey (2001, 2011) and a difference-in-differences approach to estimate the average treatment effect on multidimensional poverty. Our study finds evidence of reductions in household deprivation, mainly driven by improvements in asset ownership and dwelling infrastructure. We fail to observe significant effects on the health and education indicators. We test these findings by using propensity-score matching and inverse-probability weighting methods as robustness checks, and generally find similar estimates. In line with the literature in the field, we find heterogeneity in the results across socioeconomic groups and poverty dimensions. Further exploration suggests that household land ownership and economic activity might be driving this heterogeneity. Our work highlights the importance of using multidimensional measures to assess poverty and to empirically evaluate the impact of infrastructure projects on the development of countries, especially their rural regions.
Investigate the Neuro Mechanisms of Stereoscopic Visual Fatigue

Stereoscopic visual fatigue (SVF) due to prolonged immersion in the virtual environment can lead to negative user experience, thus hindering the development of virtual reality (VR) industry. Previous studies have focused on investigating the evaluation indicators associated with SVF, while few studies have been conducted to reveal the underlying neural mechanism, especially in VR applications. In this paper, a modified Go/NoGo paradigm was adopted to induce SVF in VR environment with Go trials for maintaining participants’ attention and NoGo trials for investigating the neural effects under SVF. Random dot stereograms (RDSs) with 11 disparities were presented to evoke the depth-related visual evoked potentials (DVEPs) during 64-channel EEG recordings. EEG datasets collected from 15 participants in NoGo trials were selected to conduct individual processing and group analysis, in which the characteristics of the DVEPs components for various fatigue degrees were compared and independent components were clustered to explore the original cortex areas related to SVF. Point-by-point permutation statistics revealed that DVEPs sample points from 230 ms to 280 ms (component P2) in most brain areas changed significantly when SVF increased. Additionally, independent component analysis (ICA) identified that component P2 which originated from posterior cingulate cortex and precuneus, was associated statistically with SVF. We believe that SVF is rather a conscious status concerning the changes of self-awareness or self-location awareness than the performance reduction of retinal image processing. Moreover, we suggest that indicators representing higher conscious state may be a better indicator for SVF evaluation in VR environments.
How Relevant Is the Information Technology Gender Gap

This paper describes research-in-progress investigating gender based wage differentials in I/T professions. The existence of these differentials is undisputed, but the significance of the “gender gap” is an area of considerable debate. The applicability of organizational and behavioral research in other areas to I/T is frequently predicated on the assumption that I/T professions are similar to other specific professions or to the economy at large. This paper describes a quantitative investigation of this assumption.
Validation and beyond: Next generation sequencing of forensic casework samples including challenging tissue samples from altered human corpses using the MiSeq FGx system

The proceeding developments in next generation sequencing (NGS) technologies enable increasing discrimination power for short tandem repeat (STR) analyses and provide new possibilities for human identification. Therefore, the growing relevance and demand in forensic casework display the need for reliable validation studies and experiences with challenging DNA samples. The presented validation of the MiSeq FGx system and the ForenSeq™ DNA Signature Prep Kit (1) investigated sensitivity, repeatability, reproducibility, concordance, pooling variations, DNA extraction method variances, DNA mixtures, degraded, and casework samples and (2) optimized the sequencing workflow for challenging samples from human corpses by testing additional PCR purification, pooling adjustments, and adapter volume reductions. Overall results indicate the system's reliability in concordance to traditional capillary electrophoresis (CE)‐based genotyping and reproducibility of sequencing data. Genotyping success rates of 100% were obtained down to 62.5 pg DNA input concentrations. Autosomal STR (aSTR) profiles of artificially degraded samples revealed significantly lower numbers of locus and allelic dropouts than CE. However, it was observed that the system still exposed drawbacks when sequencing highly degraded and inhibited samples from human remains. Due to the lack of studies evaluating the sequencing success of samples from decomposed or skeletonised corpses, the presented optimisation studies provide valuable recommendations such as an additional PCR purification, an increase in library pooling volumes, and a reduction of adapter volumes for samples with concentrations ≥31.2 pg. Thus, this research highlights the importance of all‐encompassing validation studies for implementing novel technologies in forensic casework and presents recommendations for challenging samples.