Miracle-dz/UniFAR-FTUs huggingface dataset:
Construction of a recombinant AAV vector encoding human alpha-synuclein gene with myelin basic protein Promoter

Objective: To construct recombinant adeno-associated virus carrying human α-synuclein gene under the control of cytomegalovirus (CMV) or murine myelin basic protein (MBP) promoter and to measure the virus titer and verify the recombination. Methods: Human α-Synuclein and MBP cDNA were amplified by polymerase chain reaction (PCR), digested and subcloned into shuttle plasmid pSNAV2.0 to obtain recombinant plasmid pSNAV2-CMV-α-Synuclein and pSNAV2-MBP-α-Synuclein. The resulting recombinant plasmids were co-transfected into the 293T cells by calcium-phosphate precipitation method to complete rAAV2-α-Synuclein packaging. The titer of the recombinant rAAV2-α-Synuclein was determined by dot-blot assay. The recombinant rAAV-α-Synuclein was verified by PCR of the exogenous interest genes. Results: The recombinant AAV vector containing α-Synuclein genes under the control of CMV or MBP promoter were successfully constructed. The physical particle titer of rAAV2-CMV-α-Synuclein and rAAV2-MBP-α-Synuclein were respectively 5.6×10 11 vg/ml and 3.1×10 11 vg/ml. The recombinant virus was confirmed by PCR of exogenous α-Synuclein and MBP promoter gene. Conclusion: rAAV2-α-Synuclein was successfully constructed with a high virus titer, which may offer foundation for in vitro and in vivo experiments and provide a new method for the study of pathogenetic mechanisms and exploration of new therapeutic targets of particular relevance to human neurodegenerative disorders. (Tian Huijun, Wang Xiangqing, Lang Senyang. Construction of a recombinant AAV vector encoding human alpha-synuclein gene with myelin basic protein Promoter. Life Sci J 2014;11(3):214-219). (ISSN:1097-8135). http://www.lifesciencesite. com . 30
Determination of minor actinides in irradiated fuel rod components

This study is sequential to the work presented in the AMNT meeting in 2016 concerning the measurement and simulation of several isotopes, in particular C-14 and Cs-137 in components of an irradiated PWR fuel rod segment. The current research deals with minor actinides (MA) inventory within the Zry-4 cladding sampled from the of the fuel rod segment. The relevance of MA for the fuel waste is evident; being important long lived radio-toxic contributors besides long lived fission and activation products such as I-129 and C-14. The current study shows that the amount of minor actinides is only partially due to traces of Uranium entering, during the manufacturing process, to the cladding. The major concentration of MA is found on the inner surface of the cladding and is corollary to friction between pellets and the inner surface of the cladding during the fabrication of fuel rods. Moreover, the comparison between numerical simulations and measurements lead in addition to identify the mobility strength of some isotopes like Cs-137 based on the knowledge gained from the MAs. In particular, it was shown that more than half of the Cs-137 which was found within the Zry-4 comes, most probably, from the adjacent pellets. This fact emphasizes the likelihood of Cs-137 to be released, also to the environment, under unforeseen incidents.
Assessing the Extent that the Gender and STEM Practice- Oriented Literature is Evidence-Based

One principal way to judge if the activity or program described in a practice-oriented publication is worth replicating is to consider the strength of its foundational component. A strong foundational component supports the credibility of an initiative by demonstrating that it was designed with an understanding of other similar initiatives and with evidence that there was a reasonable promise that the desired outcomes were realistically achievable. This paper reports on the findings of a mixed methods content analysis of 142 practice-oriented articles published in three different refereed venues between 1995 and 2009. A scale to measure the quality of the foundational component of the practice-oriented publications was used in the quantitative phases to test if there had been a change in the relative number and quality of practice-oriented publications over time. The validity of the scale was tested in subsequent qualitative phases of the project by an inductive examination of the characteristics of top-scoring articles. Results of the quantitative analysis indicated that the average quality rating of the foundational element of the articles was relatively low (3.37 out of a possible 6 points). The scale proved effective in distinguishing articles that were exemplary in other ways as well. The low overall quality score on the foundational component of practice-oriented publications either means that the authors of these publications are failing to provide ample documentation that these initiatives were evidence-based or the content analysis reveals a long-standing trend toward the adoption of non-evidence based practices.
Influence of Ownership Structure on Earning Quality in the Listed Firms of Tehran Stock Exchange

The main goal of this study is to analyze the relation between ownership centralization and institutional ownership with the earning quality. Studying factors which influence the earning quality can lead to better decision making by investors and it is finally arise by public awareness and also leads to proper resource allocation. Different ownership structures influence differently performance of company, earning quality, degree and manner of management control and so on. Research sample consists of the listed firms in Tehran stock exchange during 2006-2010. Independent variable in this study is ownership centralization and institutional ownership. Dependent variables (the six criteria for evaluating the earning quality) areas follows: operating cash ratio to operating earnings, accruals volume, persistent growth rate of earning, gross earnings ratio, earning persistence, receivable accounts quality. Linear regression and correlation analysis, Fisher test and t-testate used for examining the research hypotheses. Subsidiary hypotheses tests show that there is a significant relation between ownership centralization with persistent growth rate of earning and earning consistent and institutional ownership with gross earnings ratio and earning persistence, but there is no significant relation between ownership centralization and institutional ownership with the other criteria for evaluating earning quality. In general, results show that there is a positive (direct) relation between ownership centralization and institutional ownership with earning quality index, but it is not so significant.
The ecological impact of the Deepwater Horizon oil spill on Vibrio parahaemolyticus type III secretion system and the vibrio community

ix CHAPTER 1: GENERAL INTRODUCTION 1 1.1 Vibrio Background 1 1.2 Pathogenicity Factors 3 1.3 Deepwater Horizon Oil Spill 5 1.4 Vibrio parahaemolyticus and Oil 5 CHAPTER 2: IMPACT OF THE DEEPWATER HORIZON OIL SPILL ON THE VIBRIO PARAHAEMOLYTICUS TYPE THREE SECRETION SYSTEM 8 2.1 Purpose and Hypotheses 8 2.2 Materials and Methods 8 2.2.1 Sample Collection 8 2.2.1.1 Sample Locations 9 2.2.2 Sample Preparation and Plating 10 2.2.2.1 Oyster Preparation and Plating 10 2.2.2.2 Water Preparation and Plating 11 2.2.2.3 Sediment Preparation and Plating 12 2.2.3 Colony Lifting and Hybridization 13 2.2.3.1 Proteinase-K (Pro-K) 14 2.2.3.2 Hybridization 14 2.2.4 Pathogenic Vp Isolation 16 2.2. 5 DNA Extraction 17 2.2. 6 Multiplex Polymerase Chain Reaction (PCR) Screening 18 2.2. 7 Agarose Gel Electrophoresis 19 2.2. 8 Statistical Analysis 20 2.3 Results 20 2.3.1 EID Data 20 2.3.2 Presence/Absence TTSS Chi-Square Analysis 22 2.4 Discussion and Future Research 23 2.4. 1 Discussion 23 2.4. 2 Future Research 28 CHAPTER 3: IMPACT OF THE DEEPWATER HORIZON OIL SPILL ON THE VIBRIO COMMUNITY 30 3.1 Purpose and Hypotheses 30 3.2 Materials and Methods 30 3.2.1 Water Collection 30
A Critique of the Political Economy of Algorithms: A Brief History of Google’s Technological Rationality

In this article, I argue that the debate about the irrational consequences of rationality, discussed within the tradition of the Frankfurt School, and applied to technology and machinery in the concept of technological rationality (Marcuse 1941 ; 1960 ; 2007/1964 ; 2009/1965), can help us better understand and criticise contemporary algorithmic capitalism. In particular, the dialectical relation between technics and technology proposed by Marcuse (1941) can help us better understand the contexts of building digital technologies as tools for control and domi-nance. I analyse Alphabet Inc.’s (Google) documents, such as the Securities and Exchange Filing (SEC) Form 10-Ks in the period between 2004 and 2016, as well as Search Quality Rating Guidelines (SQRG) between 2016 and 2017. Based on recorded corporate growth, I argue that the company developed on the foundation of three interconnected technological rationalities: organisational rationality of flexible management values and labour utilisation ; in-formational rationality of generating value from advertising and audience labour ; and rationality of surplus value accumulation based on reification of labour and consciousness. The company produces two main types of commodities: audience commodity and algorithmic commodity, each solidifying the company’s control and dominance over Internet usage habits.
Surface Wind Regionalization in Complex Terrain

Abstract Daily wind variability in the Comunidad Foral de Navarra in northern Spain was studied using wind observations at 35 locations to derive subregions with homogeneous temporal variability. Two different methodologies based on principal component analysis were used to regionalize: 1) cluster analysis and 2) the rotation of the selected principal components. Both methodologies produce similar results and lead to regions that are in general agreement with the topographic features of the terrain. The meridional wind variability is similar in all subregions, whereas zonal wind variability is responsible for differences between them. The spectral analysis of wind variability within each subregion reveals a dominant annual cycle and the varying presence of higher-frequency contributions in the subregions. The valley subregions tend to present more variability at high frequencies than do higher-altitude sites. Last, the influence of large-scale dynamics on regional wind variability is explored by studying .
Mass and free energy of Lovelock black holes

An explicit formula for the ADM mass of an asymptotically AdS black hole in a generic Lovelock gravity theory is presented, identical in form to that in Einstein gravity, but multiplied by a function of the Lovelock coupling constants and the AdS curvature radius. A Gauss' law-type formula relates the mass, which is an integral at infinity, to an expression depending instead on the horizon radius. This and other thermodynamic quantities, such as the free energy, are then analyzed in the limits of small and large horizon radius, yielding results that are independent of the detailed choice of Lovelock couplings. In even dimensions, the temperature diverges in both limits, implying the existence of a minimum temperature for black holes. The negative free energy of sufficiently large black holes implies the existence of a Hawking–Page transition. In odd dimensions, the temperature still diverges for large black holes, which again have negative free energy. However, the temperature vanishes as the horizon radius tends to zero and sufficiently small black holes have positive specific heat.
Individual behavior towards mobile services acceptance in the airline sector: A survey in Saudi Arabia

Mobile commerce, concept commonly referred to m-commerce, has been defined by Balasubramanian et al. (2002) as any mobile electronic business that takes place, anywhere and anytime. Others, such as Kalakota and Robinson (2002), define mobile commerce as the use of wireless devices (mostly mobile phones) to perform electronic business transactions, such as product orders, money transfers, and ticket purchases. Abu Bakar and Osman (2005) defined m-commerce as the exchange of goods and services via wireless mobile phones. Varshney and Vetter (2002) have viewed m-commerce as an ecommerce using wireless devices. Khalifa and Shen (2008) argued that m-commerce is likely to experience a significant growth for a number of reasons, including the wide growth in mobile device adoption and the clear advantages of ‘anytime, anywhere’ connectivity. Moreover, the positive advantage of m-commerce is driven by its unique features and characteristics which can provide customers with added value (Siau et al, 2001; Sharma and Deng, 2002; Tang and Veijalainen, 2001) that do not exist in traditional e-commerce. These features include ubiquity, ‘anytime, anywhere’ access, personalization, flexibility, localization, and the ability to access desired information . Despite the widespread growth of mobile technologies and applications which have been developed for mobile commerce, poor user acceptance of new information technology seems to be a barrier to the successful adoption of such new technologies (Wu and Wang, 2005). Previous studies conducted to explore the constructs which affect m-commerce adoption were mostly tested in USA, China, and Taiwan (Wei et al, 2009). Thus, the purpose of this paper is to fill the present research gap in the middle east region by practically proposing and testing a model to clarify the constructs that cause customers to adopt mobile services in Saudi Arabia and especially in the airline sector. As Wei et al (2009) claimed, there is a general lack of empirical research into mobile commerce acceptance and adoption by customers .
Psychopathological symptoms in individuals at risk of Internet addiction in the context of selected demographic factors.

INTRODUCTION Researchers who study the problems of Internet addiction point out that this dependence is often co-morbid with symptoms of a variety of pathological disorders, including anxiety, depressive, somatization, and obsessive-compulsive disorders. The goal of this study was to compare the severity of psychopathological symptoms in individuals at risk of Internet addiction (according to Young's criteria) and those not at risk of developing this addiction with respect to gender and place of residence (urban vs. rural). MATERIAL AND METHODS The study included a group of 692 respondents (485 females and 207 males). The average age of the participants was 20.8 years. 56.06% of them lived in urban areas and 43.94% in rural areas. The following instruments were used: a sociodemographic questionnaire designed by the authors, Young's 20-item Internet Addiction Test (IAT, Polish translation by Majchrzak and Ogińska-Bulik), and the "O" Symptom Checklist (Kwestionariusz Objawowy "O", in Polish) by Aleksandrowicz. RESULTS Individuals at risk of Internet addiction showed significantly more severe pathological symptoms than the individuals who were not at risk of this addiction. There were differences in the severity of psychopathological symptoms between people at risk of Internet dependence living in urban and rural areas. CONCLUSIONS Individuals at risk of Internet addiction were found to be characterized by a significantly higher severity of obsessive-compulsive, conversion, anxiety, and depressive symptoms. Persons at risk of Internet addiction who lived in rural areas had significantly more severe psychopathological symptoms, mainly obsessive-compulsive, hypochondriac and phobic, compared to their urban peers.
A Brief History of Fuzzy Logic

The problems of uncertainty, imprecision and vagueness have been discussed for many years. These problems have been major topics in philosophical circles with much debate, in particular, about the nature of vagueness and the ability of traditional Boolean logic to cope with concepts and perceptions that are imprecise or vague. The Fuzzy Logic (which is usually translated into Castilian by “Logica Borrosa”, or “Logica Difusa”, but also by “Logica Heuristica”) can be considered a bypass-valued logics (Multi-valued Logic, MVL, its acronym in English). It is founded on, and is closely related to-Fuzzy Sets Theory, and successfully applied on Fuzzy Systems. You might think that fuzzy logic is quite recent and what has worked for a short time, but its origins date back at least to the Greek philosophers and especially Plato (428-347 B.C.). It even seems plausible to trace their origins in China and India. Because it seems that they were the first to consider that all things need not be of a certain type or quit, but there are a stopover between. That is, be the pioneers in considering that there may be varying degrees of truth and falsehood. In case of colors, for example, between white and black there is a whole infinite scale: the shades of gray. Some recent theorems show that in principle fuzzy logic can be used to model any continuous system, be it based in AI, or physics, or biology, or economics, etc. Investigators in many fields may find that fuzzy, commonsense models are more useful, and many more accurate than are standard mathematical ones. We analyze here the history and development of this problem: Fuzziness, or “Borrosidad” (in Castilian), essential to work with Uncertainty.
Effects of temperature and organic pollution on nutrient cycling in marine sediments

Abstract. Increasing ocean temperature due to climate change is an important anthropogenic driver of ecological change in coastal systems. In these systems sediments play a major role in nutrient cycling. Our ability to predict ecological consequences of climate change is enhanced by simulating real scenarios. Based on predicted climate change scenarios, we tested the effect of temperature and organic pollution on nutrient release from coastal sediments to the water column in a mesocosm experiment. PO43− release rates from sediments followed the same trends as organic matter mineralization rates, increased linearly with temperature and were significantly higher under organic pollution than under nonpolluted conditions. NH4+ release only increased significantly when the temperature rise was above 6 °C, and it was significantly higher in organic polluted compared to nonpolluted sediments. Nutrient release to the water column was only a fraction from the mineralized organic matter, suggesting PO43− retention and NH4+ oxidation in the sediment. Bioturbation and bioirrigation appeared to be key processes responsible for this behavior. Considering that the primary production of most marine basins is N-limited, the excess release of NH4+ at a temperature rise > 6 °C could enhance water column primary productivity, which may lead to the deterioration of the environmental quality. Climate change effects are expected to be accelerated in areas affected by organic pollution.
Fluid Dynamic Optimization of Flat Sheet Membrane Modules – Movement of Bubbles in Vertical Channels

Fouling is one of the key challenges in the operation of membrane bioreactors. Aeration is a simple but cost-intensive measure to clean the membranes. The aim of this project is to deepen the understanding of the behavior of the bubbles in such geometries to be able to optimize the cleaning of membrane systems and make them more cost-effective. Therefore, with respect to flat sheet membrane modules, the rise of a single bubble in a rectangular channel was investigated experimentally and numerically. The experimental part was performed with a high speed camera and the images were analyzed with the help of NI LabView, Vision Development Module and MS Excel. The software was used to determine the centre of mass of the bubble on each image which provides the rising path when combined. This rising path is determined for different parameter combinations varied bubble sizes and superimposed liquid velocities. The same parameter combinations were investigated numerically with the help of Ansys Fluent. Based on the rising paths, a comparison of the simulation and experiments is possible. Specifically the terminal rise velocity, the oscillation frequency and the amplitude are used for comparison.
A Survey on different techniques of steganography

Steganography is important due to the exponential development and secret communication of potential computer users over the internet. Steganography is the art of invisible communication to keep secret information inside other information. Steganalysis is the technology that attempts to ruin the Steganography by detecting the hidden information and extracting.Steganography is the process of Data embedding in the images, text/documented, audio and video files. The paper also highlights the security improved by applying various techniques of video steganography.
Three phase inverter for induction motor control using fuzzy-pi controller with Arduino

In this project, the main focus is the development of the three phase inverter for induction motor using Fuzzy-PI controller with Arduino. The Fuzzy-PI controller is designed using the Matlab’s Simulink program software. The purpose of the controller is to control the current fed to the induction motor during motor operation. Using this Fuzzy-PI control algorithm, the speed of the running motor can be adjust depend on the DC voltage input which also effect the current flow into the induction motor. There are two situations being test in this project, which is open loop and close loop control scheme. Both situations are covered in both simulation and real hardware testing. The result of the open loop testing and the close loop testing are being compared as the performance indicator. The close loop control scheme show a good ability and responsive in current control for induction motor operation as compared to open loop control.
An evaluation of the impact of industrial restructuring on individual human capital accumulation in France (1956-1993)

This article evaluates the effect of French industrial restructuring during 1956-1993, on individual human capital accumulation. We use data from the French Training and Occupational Skills survey and the Population Census (INSEE). We estimate a human capital production function using two econometric strategies (controlling for covariates; instrumental variables). We show that industrial restructuring has a negative impact on individual human capital accumulation for the children of blue-collar workers.
Writing Activities Embedded in Bioscience Laboratory Courses to Change Students' Attitudes and Enhance Their Scientific Writing.

We introduced writing activities into a project style third year undergraduate biomolecular science laboratory to assist the students to produce a final report in the form of a journal article. To encourage writing while the experimental work was proceeding, the embedded writing activities required ongoing analysis of experimental data. After formative feedback from peers and teaching staff, the revised work was incorporated directly into the prepared journal framework. Surveys of student attitudes showed significant improvements in confidence in their ability to write an introduction, produce figure legends, distinguish results from discussion and generate a logical flow of information. Attitudes towards report writing became significantly more positive and the training was highly rated. Key factors that contributed to the perceived benefits were use of the student’s own data, the chance to incorporate revised work directly into their reports and the realisation that they were performing tasks of direct relevance to their future careers.