Miracle-dz/UniFAR-FTUs huggingface dataset:
Rearing method for the endangered species Dineutus mellyi mellyi Régimbart, 1882 (Coleoptera: Gyrinidae)

Abstract Rearing research is being conducted to conserve many endangered aquatic Coleoptera species; however, studies on Gyrinidae species are scarce. Herein, we present an efficient rearing method for the endangered species Dineutus mellyi mellyi Régimbart, 1882 (Coleoptera: Gyrinidae), practiced at the Ishikawa Insect Museum. The survival rate of the larvae reared using this method at different developmental stages was as follows: first-instar larvae, 100% (n = 26); second-instar larvae, 100% (n = 26); third-instar larvae, 92% (n = 24); landing to pupate, 73% (n = 19); pupa, 73% (n = 19); and emergence to escape from pupal chamber, 69% (n = 18). Therefore, we recommend this method for sustainable ex situ conservation of this species. This method can be generalised to other Gyrinidae and aquatic Coleoptera species.
NASA Satellite Measurements Show Global‐Scale Reductions in Free Tropospheric Ozone in 2020 and Again in 2021 During COVID‐19

NASA satellite measurements show that ozone reductions throughout the Northern Hemisphere (NH) free troposphere reported for spring‐summer 2020 during the COronaVIrus Disease 2019 pandemic have occurred again in spring‐summer 2021. The satellite measurements show that tropospheric column ozone (TCO) (mostly representative of the free troposphere) for 20°N–60°N during spring‐summer for both 2020 and 2021 averaged ∼3 Dobson Units (DU) (or ∼7%–8%) below normal. These ozone reductions in 2020 and 2021 were the lowest in the 2005–2021 record. We also include satellite measurements of tropospheric NO2 that exhibit reductions of ∼10%–20% in the NH in early spring‐to‐summer 2020 and 2021, suggesting that reduced pollution was the main cause for the low anomalies in NH TCO in 2020 and 2021. Reductions of TCO ∼2 DU (7%) are also measured in the Southern Hemisphere in austral summer but are not associated with reduced NO2.
BRCA Mutations in Ovarian and Prostate Cancer: Bench to Bedside

Simple Summary DNA damage is one of the hallmarks of cancer. Epithelial ovarian cancer (EOC) —especially the high-grade serous subtype—harbors a defect in at least one DNA damage response (DDR) pathway. Defective DDR results from a variety of lesions affecting homologous recombination (HR) and nonhomologous end joining (NHEJ) for double strand breaks, base excision repair (BER), and nucleotide excision repair (NER) for single strand breaks and mismatch repair (MMR). Apart from the EOC, mutations in the DDR genes, such as BRCA1 and BRCA2, are common in prostate cancer as well. Among them, BRCA2 lesions are found in 12% of metastatic castration-resistant prostate cancers, but very rarely in primary prostate cancer. Better understanding of the DDR pathways is essential in order to optimize the therapeutic choices, and has led to the design of biomarker-driven clinical trials. Poly(ADP-ribose) polymerase (PARP) inhibitors are now a standard therapy for EOC patients, and more recently have been approved for the metastatic castration-resistant prostate cancer with alterations in DDR genes. They are particularly effective in tumours with HR deficiency. Abstract DNA damage repair (DDR) defects are common in different cancer types, and these alterations can be exploited therapeutically. Epithelial ovarian cancer (EOC) is among the tumours with the highest percentage of hereditary cases. BRCA1 and BRCA2 predisposing pathogenic variants (PVs) were the first to be associated with EOC, whereas additional genes comprising the homologous recombination (HR) pathway have been discovered with DNA sequencing technologies. The incidence of DDR alterations among patients with metastatic prostate cancer is much higher compared to those with localized disease. Genetic testing is playing an increasingly important role in the treatment of patients with ovarian and prostate cancer. The development of poly (ADP-ribose) polymerase (PARP) inhibitors offers a therapeutic strategy for patients with EOC. One of the mechanisms of PARP inhibitors exploits the concept of synthetic lethality. Tumours with BRCA1 or BRCA2 mutations are highly sensitive to PARP inhibitors. Moreover, the synthetic lethal interaction may be exploited beyond germline BRCA mutations in the context of HR deficiency, and this is an area of ongoing research. PARP inhibitors are in advanced stages of development as a treatment for metastatic castration-resistant prostate cancer. However, there is a major concern regarding the need to identify reliable biomarkers predictive of treatment response. In this review, we explore the mechanisms of DDR, the potential for genomic analysis of ovarian and prostate cancer, and therapeutics of PARP inhibitors, along with predictive biomarkers.
Molecular Identification of Sarcocystis Species in Sheep from Lithuania

Simple Summary Members of the genus Sarcocystis (Apicomplexa: Sarcocystidae) are protozoans that have a two-host, prey–predator life cycle. These parasites are most thoroughly examined in economically important animals. Several Sarcocystis species are known to form macroscopic and microscopic sarcocysts in the muscle tissues of sheep. A previous study carried out in Lithuania has shown a maximum Sarcocystis infection prevalence in sheep and a high parasite burden; however, species were not identified. In the present study, diaphragm, oesophagus, and heart samples of 69 sheep raised in Lithuania were examined. No macrocysts were detected in the sheep muscles, while the microcysts found corresponded to two morphological types. By molecular methods, two Sarcocystis species, S. arieticanis and S. tenella, using canids as their definitive hosts, were identified for the first time in sheep from Lithuania. Both species were detected in all the studied animals. In summary, very high detection rates of S. arieticanis and S. tenella ranging from 82.61% to 100% were established in the examined diaphragm, oesophagus, and heart muscle samples of sheep. Abstract Data on the distribution of different Sarcocystis species in various muscles of sheep are scarce. In the present study, 190 diaphragm, oesophagus, and heart muscle samples of 69 sheep raised in Lithuania were examined for the presence of Sarcocystis spp. Under a light microscope, two morphological types of microcysts corresponding to S. arieticanis and S. tenella were detected. Eight and 12 sarcocysts of S. arieticanis and S. tenella, respectively, were isolated and characterised by the sequencing of a portion of cox1. The sequence comparisons revealed the highest similarity between European and Asian isolates of S. arieticanis and S. tenella obtained from domestic sheep and other wild Caprinae hosts. Based on peptic digestion, nested PCR targeting cox1, and sequencing, a 100% infection prevalence of S. arieticanis and S. tenella was observed in the 69 studied animals. The occurrence of S. tenella was significantly higher in the diaphragm than in the oesophagus (χ2 = 13.14, p < 0.001), whereas differences in the prevalence of S. arieticanis in the studied muscle types were insignificant (χ2 = 1.28, p > 0.05). Further molecularly based epidemiological studies are needed to compare the prevalence of Sarcocystis species in various muscles of sheep raised in different geographic regions.
Event-triggered control of positive switched systems with randomly occurring state saturation

This paper is concerned with the event-triggered control of positive switched systems with randomly occurring state saturation. First, a vector-based event-triggering condition is established. Thus, interval and polytope approaches are used to deal with the positivity and stability of the systems and the saturation, respectively. An event-triggered controller and the corresponding domain of attraction are designed by decomposing gain matrix into the sum of non-negative and non-positive components. The proposed design approach is developed for the systems with input saturation. Finally, two examples are given to illustrate the effectiveness of the proposed design.
Inclusion of IoT technology in additive manufacturing: Machine learning-based adaptive bead modeling and path planning for sustainable wire arc additive manufacturing and process optimization

Industrial civilization transforms current cutting edge technologies and the evolution of Industry 5.0 is more aggressive with the use of IoT-enabled smart machines and robots in the manufacturing sector today. IoT technology deals with digital data as in additive manufacturing (AM). The potential and progressive aspects of AM embarks for functional part development instead of initial prototyping. AM is one of large-scale production with less buy-to-fly (BTF) ratio. In the present work, a novel framework has been proposed and utilized to attain adaptive bead modeling and an appropriate path plan for enhanced deposition and surface quality of weld beads. Further, the influence of input process parameters toward sustainable wire arc additive manufacturing (WAAM) is also focused. Machine learning-based hybrid-TLBO (h-TLBO) and support vector machine (SVM) is deployed for the optimization process. With the aid of graph theory, weights are estimated for h-TLBO. The overall process parameters and entire data module is handled with IoT technology and can be accessed for processing. The simulated post-processing results are validated experimental test results and found to be in good concurrence.
Ethical Statistics and Statistical Ethics: Making an Interdisciplinary Module

This article describes an innovative curriculum module the first author created on the two-way exchange between statistics and applied ethics. The module, having no particular mathematical prerequisites beyond high school algebra, is part of an undergraduate interdisciplinary ethics course which begins with a 3-week introduction to basic applied ethics taught by a philosophy professor (the second author), and continues with 3-week modules from professors in various other disciplines. The first author's module's emphasis on conceptual and critical thinking makes it easily adaptable to service-level courses as well as readily expandable for more mathematically sophisticated audiences. Through in-class explorations and discussions, the module made connections to contemporary topics such as the death penalty, equal pay for equal work, and profiling. This article shares examples, resources, strategies and lessons learned for instructors wishing to develop their own modules of various lengths.
The Provenance of Ancient Cotton and Wool Textiles from Nubia: Insights from Technical Textile Analysis and Strontium Isotopes

Late antique and medieval cotton and wool textiles found in the middle Nile Valley (Nubia, northern Sudan) were analysed for their technical characteristics and strontium (Sr) isotope composition. All wool textiles exhibit Sr isotope signatures consistent with the isotopic background of the region studied and are considered to be of local origin. However, a medieval wool kilim from Meinarti shows technical and aesthetic features suggesting its foreign Maghreb provenance. As this fabric dates back to the occupation of Meinarti by the Beni Ikrima tribe, it is suggested that the kilim was woven by the Beni Ikrima people from local Nubian raw material. The cotton samples tested come from abroad and document trade with the oases of the Egyptian Western Desert, the west coast of India, and perhaps also with the Arabian Peninsula or Pakistan.
The “Infernaccio” Gorges: Microbial Diversity of Black Deposits and Isolation of Manganese-Solubilizing Bacteria

Simple Summary “Infernaccio” gorges are one of the Earth’s hidden habitats in Central Italy. Beyond the deep incisions and high slopes, these gorges are characterized by black deposits in gorge walls and covering rock surfaces. Several geological events have shaped these unique geological formations and their microbiota. This study investigated microbial contribution to black deposit formation and isolating Mn-oxide-solubilizing bacteria. Our results provided evidence of the putative role of Bacteria and Archaea in forming manganese oxide deposits. Findings also showed that these deposits are a source of valuable strains with manganese oxide bioleaching properties, essential for bioremediation and metal recovery. Abstract The present study explored the microbial diversity of black deposits found in the “Infernaccio” gorge. X-ray Powdered Diffraction (XRPD) was used to investigate the crystallinity of the samples and to identify the minerals. Scanning electron microscope and energy-dispersive X-ray spectroscopy (SEM-EDS) were used to detect the bacterial imprints, analyze microbe–mineral interactions, and highlight the chemical element distribution in the black deposits. 16S rRNA gene metabarcoding allowed the study of Archaea and Bacteria communities. Mn-oxide-solubilizing isolates were also obtained and characterized by culturable and molecular approaches. The multidisciplinary approach showed the occurrence of deposits composed of birnessite, diopside, halloysite, and leucite. Numerous bacterial imprints confirmed the role of microorganisms in forming these deposits. The Bacteria and Archaea communities associated with these deposits and runoff waters are dynamic and shaped by seasonal changes. The uncultured and unknown taxa are the most common and abundant. These amplicon sequence variants (ASVs) were mainly assigned to Proteobacteria and Bacteroidetes phyla. Six isolates showed interesting Mn solubilization abilities under microaerophilic conditions. Molecular characterization associated isolates to Brevibacterium, Bacillus, Neobacillus, and Rhodococcus genera. The findings enriched our knowledge of geomicrobiological aspects of one of the Earth’s hidden habitats. The study also unveiled the potential of this environment as an isolation source of biotechnologically relevant bacteria.
Creatine kinase and alpha-fetoprotein as biochemical markers in diagnosis of placenta increta and percreta

Background: placenta accreta is a life-threatening pregnancy complication which usually accompanied with placenta previa. It is important to make the diagnosis before delivery therefore a preoperative planning will decrease complications. The aim of this study: was to compare between creatine kinase and α-fetoprotein as biological markers with ultrasound & Doppler findings for the sake of prenatal detection of morbid placentation in anterior placenta on scar of previous cesarean section. Patients and Methods: The present study was Prospective cohort observational study carried out at Beni Suef University Maternity Hospital and MUST Teaching Hospital. Women were recruited from the outpatient obstetrics clinic or emergency room and admitted to antepartum inpatient high risk service. Results: This study showed a significant increase in both CK (p value =0.014) and msAFP (P value=0.028) levels in cases compared to the control group.
Influence of Mesoporous Iron Based Nanoparticles on Chlorella Sorokiniana Metabolism During Photosynthetic Biogas Upgrading

Three different mesoporous iron based nanoparticles (NPs) were added to Chlorella sorokiniana batch cultures devoted to photosynthetic biogas upgrading to enhance CO 2 biofixation: Fe 2 O 3 , carbon coated zero valent iron NPs containing 7.26 % (wt%) of iron (CALPECH NPs) and carbon coated zero valent iron NPs containing 31.38 % (wt%) of iron (SMALLOPS NPS). The three types of NPs enhanced CO 2 adsorption and therefore biogas upgrading in tests conducted in 1.2 L enclosed photobioreactors. However, the addition of 70 mg L (cid:0) 1 of CALPECH NPs resulted in a 2-fold enhancement in the microalgae productivity, and a carbohydrate and lipid content increase by 56 % and 25 %, respectively compared to the control assay. Under UV and visible light irradiation the addition of Fe 2 O 3 NPs resulted in a 5.3-times fold enhancement in carbohydrate content when 70 mg L (cid:0) 1 was added. Similarly, the addition of 20 mg L (cid:0) 1 of SMALLOPS NPs increased the lipid content by 11 % under UV irradiation. Interestingly, CALPECH NPs did not significantly influence C. sorokiniana composition , suggesting that these particular NPs can act as UV scavengers. Therefore, the addition of mesoporous nanoparticles could improve CO 2 fixation and microalgae productivity during photosynthetic biogas upgrading.
The nomogram for the prediction of overall survival in patients with metastatic lung adenocarcinoma undergoing primary site surgery: A retrospective population-based study

Background Lung adenocarcinoma (LUAD) is the most common type of Non-small-cell lung cancer (NSCLC). Distant metastasis of lung adenocarcinoma reduces the survival rate. we aim to develop a nomogram in order to predict the survival of patients with metastatic lung adenocarcinoma. Methods We retrospectively collected patients who were initially diagnosed as metastatic LUAD from 2010 to 2015 from SEER database. Based on the multivariate and univariate Cox regression analysis of the training cohorts, independent prognostic factors were assessed. The nomogram prediction model was then constructed based on these prognostic factors to predict the overall survival at 12, 24 and 36 months after surgery. Nomogram were identified and calibrated by c-index, time-dependent receiver operating characteristic curve (time-dependent AUC) and calibration curve. Decision curve analysis (DCA) was used to quantify the net benefit of the nomogram at different threshold probabilities, and to better compare with the TNM staging system, we calculated the c-index of this nomogram as well as the net reclassification improvement (NRI) and the integrated discrimination improvement (IDI). Result A total of 1102 patients with metastatic LUAD who met the requirements were included for analysis. They were randomly divided into 774 in the training cohorts and 328 in the validation cohorts. As can be seen from the calibration plots, the predicted nomogram and the actual observations in both of the training and validation cohorts were generally consistent. The time dependent AUC values of 12 months, 24 months and 36 months were 0.707, 0.674 and 0.686 in the training cohorts and 0.690, 0.680 and 0.688 in the verification cohorts, respectively. C-indexes for the training and validation cohorts were 0.653 (95%CI 0.626-0.68)and 0.663 (95%CI 0.626-1), respectively. NRI and IDI show that the model is more clinical applicable than the existing staging system. In addition, our risk scoring system based on Kaplan Meier (K-M) survival curve can accurately divide patients into three hierarchy risk groups. Conclusion This has led to the development and validation of a prognostic nomogram to assist clinicians in determining the prognosis of patients with metastatic lung adenocarcinoma after primary site surgery.
Fluidic rolling robot using voltage-driven oscillating liquid

Rolling motions have been observed in many animals and insects. In the previous fluidic rolling system, a deformed chamber and long cables were imperative to drive the soft rolling actuators, which required high pressure and a sophisticated controlling strategy. In this study, we propose a soft fluidic roller using a simple structure composed of a bendable and twistable electrohydrodynamic (EHD) pump and a layer of natural latex. To realize the rolling motion, we first optimized the electrode and channel height of the EHD pumps using different patterns and designs. We also examined the output power, efficiency, pressure loss, bending, and twisting performance. Subsequently, the optimized electrodes and channel height were selected to design the power source of the EHD roller. This roller was lightweight (0.7 g) with an amount of liquid (0.6 g). This EHD robot can roll as the EHD liquid oscillates under a duty-controlled voltage realized using a high-voltage circuit. Next,we investigated the influence of frictional forces on rolling performance. Finally, the rolling motion in the liquid was demonstrated. This study extends the EHD pumps to independent soft actuators integrated with a soft power source.
Bergenin attenuates TNF-α-induced oxidative stress and inflammation in HaCaT cells by activating Nrf2 pathway and inhibiting NF-κB pathway

Purpose: To investigate the effect of bergenin in TNF-α-induced human immortalized keratinocytes.Methods: Human immortal keratinocyte cells (HaCaT) were incubated with tumor necrosis factor-α (TNF-α), and then treated with increasing concentrations of bergenin. Cell viability was determined using Cell Counting Kit-8 (CCK8), while oxidative stress and inflammation were evaluated using enzyme linked immunosorbent assay (ELISA). Results: Incubation with increasing concentrations of bergenin showed no significant effect on cell viability of HaCaT (p < 0.05). Tumor necrosis factor-α significantly induced up-regulation of interleukin (IL)-6 and IL-8 in HaCaT (p < 0.001), while bergenin significantly reduced the levels of IL-6 and IL-8 (p < 0.001). Bergenin also attenuated TNF-α-induced decrease in superoxide dismutase (SOD), as well as increase in malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS). Protein expression of IκBα in HaCaT was decreased, while p-p65 and p-IκBα were increased by treatment with TNF-α. However, bergenin increased IκBα but decreased p-p65 and p-IκBα in TNF-α-induced HaCaT. Moreover, bergenin reduced Kelch-like ECH-associated protein 1 (Keap1), while enhancing transcription factor NF-E2 p45-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in TNF-α-induced HaCaT.Conclusion: Bergenin exerts antioxidant and anti-inflammatory effects on TNF-α-induced HaCaT by activating Nrf2 pathway and inactivating NF-κB pathway. Therefore, bergenin is a potential agent for the treatment of psoriasis.
Drug Awareness Campaign: A Preliminary Study on The Effectiveness of Electronic Media Strategy among Youth in Penang

A Preliminary Study on The Effectiveness of Electronic Abstract The evolution of media and communications makes information delivery faster. Media and communications are utilized for different purposes including for public health awareness campaign. However, studies evaluating the effectiveness of media and communications in public health awareness campaigns, particularly those that are related to the illicit substance usage awareness are still limited. Hence, this preliminary study aims to evaluate the effectiveness of the usage of electronic media in Drug Awareness Campaign. A cross-sectional survey was conducted involving a total of 50 participants consisting of youths aged 18 to 29. Participants were recruited through convenience sampling method. The findings of the study indicated that the effectiveness of the message strategies, language strategies and media channels strategies are at high level. Meanwhile, the effectiveness of the time strategy and presentation strategy reached a moderately-high level. It is vital that future studies attempt to identify innovation and transformation approaches that can be undertaken to enhance the effectiveness of media and communication strategies towards the Drug Awareness Campaign.