Miracle-dz/UniFAR-FTUs huggingface dataset:
Improvement in prefrontal thalamic connectivity during the early course of the illness in recent-onset psychosis: a 12-month longitudinal follow-up resting-state fMRI study

Abstract Background Previous research in resting-state functional magnetic resonance imaging (rs-fMRI) has shown a mixed pattern of disrupted thalamocortical connectivity in psychosis. The clinical meaning of these findings and their stability over time remains unclear. We aimed to study thalamocortical connectivity longitudinally over a 1-year period in participants with recent-onset psychosis. Methods To this purpose, 129 individuals with recent-onset psychosis and 87 controls were clinically evaluated and scanned using rs-fMRI. Among them, 43 patients and 40 controls were re-scanned and re-evaluated 12 months later. Functional connectivity between the thalamus and the rest of the brain was calculated using a seed to voxel approach, and then compared between groups and correlated with clinical features cross-sectionally and longitudinally. Results At baseline, participants with recent-onset psychosis showed increased connectivity (compared to controls) between the thalamus and somatosensory and temporal regions (k = 653, T = 5.712), as well as decreased connectivity between the thalamus and left cerebellum and right prefrontal cortex (PFC; k = 201, T = −4.700). Longitudinal analyses revealed increased connectivity over time in recent-onset psychosis (relative to controls) in the right middle frontal gyrus. Conclusions Our results support the concept of abnormal thalamic connectivity as a core feature in psychosis. In agreement with a non-degenerative model of illness in which functional changes occur early in development and do not deteriorate over time, no evidence of progressive deterioration of connectivity during early psychosis was observed. Indeed, regionally increased connectivity between thalamus and PFC was observed.
Measurement of Cation Exchange Capacity of Organic‐Matter Fractions from Soils using a Modified Compulsive Exchange Method

Abstract This article describes a modified compulsive exchange method suitable for the determination of cation exchange capacity (CEC) over a pH range on solid, separated organic‐matter fractions. The method is demonstrated to be a reproducible and relatively simple means of measuring CEC on fractions separated by physical means in conjunction with hydrofluoric acid (HF) treatment. By including a modification for measuring the residual magnesium (Mg2+) content by dilution and conductivity, the method is simplified to measure CEC versus pH using a single sample; the only laboratory instruments required are pH and conductivity meters and a balance. The lack of agreement between this method and the silver thiourea (AgTU) method for some fractions at low pH is attributed to the uncontrolled variation in solution ionic strength when acid is introduced to effect solution pH changes in the AgTU method.
Physiologically Based Pharmacokinetic Modeling of Metoprolol Enantiomers and α-Hydroxymetoprolol to Describe CYP2D6 Drug-Gene Interactions

The beta-blocker metoprolol (the sixth most commonly prescribed drug in the USA in 2017) is subject to considerable drug–gene interaction (DGI) effects caused by genetic variations of the CYP2D6 gene. CYP2D6 poor metabolizers (5.7% of US population) show approximately five-fold higher metoprolol exposure compared to CYP2D6 normal metabolizers. This study aimed to develop a whole-body physiologically based pharmacokinetic (PBPK) model to predict CYP2D6 DGIs with metoprolol. The metoprolol (R)- and (S)-enantiomers as well as the active metabolite α-hydroxymetoprolol were implemented as model compounds, employing data of 48 different clinical studies (dosing range 5–200 mg). To mechanistically describe the effect of CYP2D6 polymorphisms, two separate metabolic CYP2D6 pathways (α-hydroxylation and O-demethylation) were incorporated for both metoprolol enantiomers. The good model performance is demonstrated in predicted plasma concentration–time profiles compared to observed data, goodness-of-fit plots, and low geometric mean fold errors of the predicted AUClast (1.27) and Cmax values (1.23) over all studies. For DGI predictions, 18 out of 18 DGI AUClast ratios and 18 out of 18 DGI Cmax ratios were within two-fold of the observed ratios. The newly developed and carefully validated model was applied to calculate dose recommendations for CYP2D6 polymorphic patients and will be freely available in the Open Systems Pharmacology repository.
Toward a better definition of hematopoietic progenitors suitable for B cell differentiation

The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B cells exert roles as effector and as regulator of immune responses in different clinical settings, we were interested in generating B cells from hIPSC. We differentiated human embryonic stem cells (hESC) and hIPSC into B cells onto OP9 and MS-5 stromal cells successively. We overcame issues in generating CD34+CD43+ hematopoietic progenitors with appropriate cytokine conditions and emphasized the difficulties to generate proper hematopoietic progenitors. We highlight CD31intCD45int phenotype as a possible marker of hematopoietic progenitors suitable for B cell differentiation. Defining precisely proper lymphoid progenitors will improve the study of their lineage commitment and the signals needed during the in vitro process.
Membrane properties of porous and expanded poly(tetrafluoroethylene) films grafted with hydrophilic monomers and their permeation behavior

Porous and expanded poly(tetrafluoroethylene) (pPTFE and ePTFE) films grafted with methacrylic acid (MAA) and 2-(dimethylamino)ethyl methacrylate (DMA) were prepared by the combined use of the plasma treatment and photografting. Their membrane properties and permeation behavior to saccharides and dextrans were investigated. Water absorptivity and electrical conductivity considerably increased around the grafted amounts at which the individual grafted polymer chains reached the center of each PTFE film. The glucose permeability of all four types of grafted pPTFE and ePTFE films showed the maximum values at pH 8, and grafted ePTFE films had higher permeabilities than grafted pPTFE films. It was made clear from the viscometric measurements of the aqueous solutions of polyMAA (PMAA) and polyDMA (PDMA) that the glucose permeability of MAA-grafted pPTFE (MAA-g-pPTFE) and MAA-grafted ePTFE (MAA-g-ePTFE) films increased more as grafted PMAA chains expanded. Those of DMA-grafted pPTFE (DMA-g-pPTFE) and DMA-grafted ePTFE (DMA-g-ePTFE) films increased more as grafted PDMA chains contracted. These results explain that the glucose permeability of the grafted pPTFE and ePTFE films depend not only on the grafted amount but also on the distribution of the corresponding grafted polymer chains in the direction of depth inside both PTFE films. The on-off regulation of permeation for DMA-g-pPTFE and DMA-g-ePTFE films could be repeated in response to the alternate temperature change and their on-off regulation patterns were completely opposite to those of many other hydrogel samples consisting of thermosensitive polymers with lower critical solution temperatures. In addition, it was found from permeation experiments, using three types of saccharides and dextrans with different molecular weights as a permeant, that permeation flux of DMA-g-ePTFE films decreased with an increase in molecular weight of the permeant. Separation factors showed the maximum value at the grafted amount of 0.8 mmol/g. The tensile strength measurements showed the grafted pPTFE and ePTFE films possessed adequate strength in the waterswollen state to be of use for a functional membrane. DMA-g-ePTFE films are most suitable for a separation membrane of polymeric species of four grafted PTFE films. © 1996 John Wiley & Sons, Inc.
Wireless Transmission Method for Large Data Based on Hierarchical Compressed Sensing and Sparse Decomposition

With the widespread application of wireless sensor networks, large-scale systems with high sampling rates are becoming more and more common. The amount of original data generated by the wireless sensor network is very large, and transmitting all the original data back to the host wastes network bandwidth and energy. This paper proposes a wireless transmission method for large data based on hierarchical compressed sensing and sparse decomposition. This method includes a hierarchical signal decomposition method based on the same sparse basis and different sparse basis hierarchical compressed sensing method with a mask. Compared with the traditional compressed sensing method, this method reduces the error of signal reconstruction, reduces the amount of calculation during signal reconstruction, and reduces the occupation of hardware resources. We designed comparison experiments between the traditional compressed sensing algorithm and the method proposed in this article. In addition, the experiments’ results prove that our proposed method reduces the execution time, as well as the reconstruction error, compared with the traditional compressed sensing algorithm, and it can achieve better reconstruction at a relatively low compression ratio.
Short-Term Outcomes of Polycarbophil and Propionibacterium acnes Lysate Gel after Open Hemorrhoidectomy: A Prospective Cohort Study

Background: Pain is the most common complication after open excisional hemorrhoidectomy (OEH). We assessed the effectiveness of polycarbophil and Propionibacterium acnes lysate gel (Emorsan®Gel) on pain control after OEH. Research design and methods: Fifty consecutive patients undergoing OEH were included. All patients received stool softeners and oral analgesia in the post-operative period. Emorsan®Gel was also used topically by the last 25 patients (Emorsan®Gel group (EG)) until Post-Operative Day 20 (POD 20). The primary outcome was the effectiveness of Emorsan®Gel on pain relief using an 11-point visual analogue scale (VAS). Morbidity, wound healing (WH), and time to work were documented at POD 1, POD 10, POD 20, and POD 40. Results: Of the 50 patients enrolled, twenty-eight (56%) were males; median age, 49 (range, 28–73) years. The VAS score decreased over time in all patients, with significantly lower scores at POD 20 in the EG (1.44 (SD, 1.16) vs. 2.12 (0.93) in the control group (CG); p = 0.045). All patients in the EG achieved complete WH at last follow-up, compared to only 17 (68%) in the CG (p = 0.004). The likelihood of WH was 66% higher in the EG (OR, 1.66 [95%CI, 0.80–3.44; p = 0.172). Conclusions: Emorsan®Gel is safe and effective at reducing pain after EOH, promoting earlier WH compared to standard care treatment.
Chlorogenic Acid Supplementation Benefits Zebrafish Embryos Exposed to Auranofin

Antioxidant supplementation may potentially be beneficial for embryonic development to reduce complications associated with increased levels of oxidative stress. Chlorogenic acid, one of the key polyphenolic antioxidants in S. oleraceus, was evaluated for potential protective effects during embryonic development of zebrafish exposed to the teratogen auranofin. Zebrafish embryos were transiently exposed to auranofin to induce developmental abnormalities. Phenotypic abnormalities were scored based on their severity at day 5 post-fertilization. The embryos supplemented with 250 µM chlorogenic acid showed a significantly lower score in phenotypic abnormalities compared to non-supplemented embryos after auranofin exposure. Therefore, supplementation with a low dose of chlorogenic acid showed a protective effect from auranofin-induced deformities and encouraged normal growth in zebrafish embryos. This study provides further support for the potential of using antioxidant supplementation during embryonic development for protection against malformation.
The protein tyrosine phosphatase PTP-PEST mediates hypoxia-induced endothelial autophagy and angiogenesis via AMPK activation

ABSTRACT Global and endothelial loss of PTP-PEST (also known as PTPN12) is associated with impaired cardiovascular development and embryonic lethality. Although hypoxia is implicated in vascular remodelling and angiogenesis, its effect on PTP-PEST remains unexplored. Here we report that hypoxia (1% oxygen) increases protein levels and catalytic activity of PTP-PEST in primary endothelial cells. Immunoprecipitation followed by mass spectrometry revealed that α subunits of AMPK (α1 and α2, encoded by PRKAA1 and PRKAA2, respectively) interact with PTP-PEST under normoxia but not in hypoxia. Co-immunoprecipitation experiments confirmed this observation and determined that AMPK α subunits interact with the catalytic domain of PTP-PEST. Knockdown of PTP-PEST abrogated hypoxia-mediated tyrosine dephosphorylation and activation of AMPK (Thr172 phosphorylation). Absence of PTP-PEST also blocked hypoxia-induced autophagy (LC3 degradation and puncta formation), which was rescued by the AMPK activator metformin (500 µM). Because endothelial autophagy is a prerequisite for angiogenesis, knockdown of PTP-PEST also attenuated endothelial cell migration and capillary tube formation, with autophagy inducer rapamycin (200 nM) rescuing angiogenesis. In conclusion, this work identifies for the first time that PTP-PEST is a regulator of hypoxia-induced AMPK activation and endothelial autophagy to promote angiogenesis. Highlighted Article: The protein tyrosine phosphatase PTP-PEST is identified as a major regulator of hypoxia-induced AMPK activation in endothelial cells, which consequently leads to autophagy and angiogenesis.
Development and Characterization of an Orodispersible Film for Vitamin D3 Supplementation

Vitamin D plays a crucial and very well-known role in regulation of calcium homeostasis and bone metabolism and mineralization. However, a huge and more recent body of evidence supports the positive influence of vitamin D on the regulation of immune response, ranging from protection against respiratory tract infections to prevention and management of asthma. Nevertheless, vitamin D deficiency is a very common condition and there is an increasing need for suitable products for proper supplementation, allowing good compliance also in specific populations. Orally disintegrating tablets (ODT) were first developed to overcome the difficulty experienced by pediatric and geriatric patients of swallowing traditional oral dosage forms and, recently, orodispersible films (ODF) are gaining popularity as novel dosage form for assuming active pharmaceutical ingredients, vitamins, and ingredients for food supplements. This study describes a 2000 IU Vitamin D3 ODF for daily intake, consisting of hydrophilic polymers and suitable excipients, manufactured by film-casting process. Elongation-at-break (E%), Young’s modulus (Y), and tensile strength (TS) were investigated using a dynamometer. Chemical stability was evaluated assaying the vitamin D3 in the films stored at different environmental conditions. In addition, in vitro disintegration and dissolution studies were performed. Correlation existed between the mechanical properties of the film and the residual water, acting as plasticizer. The stability study showed that vitamin D3 assay was ≥90% also after 3 months at 40 °C. The film disintegrated in less than 1 min and the vitamin D3 released was ≥75% after 15 min. An ODF with suitable properties can be manufactured and used as innovative dosage form for vitamin D3 food supplements.
Silicon quantum dots in diamond matrix: a new synthesis route†

Light-emitting layers of silicon quantum dots embedded in a diamond matrix have been prepared by using a new hybrid chemical vapour deposition-powder flowing technique. The polycrystalline diamond matrix is grown by thermal activation of CH 4 -H 2 mixtures. The silicon nanoparticles used for the synthesis of the diamond-based composites were prepared by CO 2 laser pyrolysis of SiH 4 -He mixtures in a gas flow reactor with controlled atmosphere. During the matrix deposition, the powder-flowing apparatus drives the silicon nanoparticles across the active area of the substrate, where diamond is growing. A strong room temperature emission is observed from composite films with characteristics dependent on the size distribution of the inserted silicon particles. We report details of the synthesis procedure and emission features of composite diamond films, whose properties promise high technological impact.
Involvement of the FAK Network in Pathologies Related to Altered Mechanotransduction

Mechanotransduction is a physiological process in which external mechanical stimulations are perceived, interpreted, and translated by cells into biochemical signals. Mechanical stimulations exerted by extracellular matrix stiffness and cell–cell contacts are continuously applied to living cells, thus representing a key pivotal trigger for cell homeostasis, survival, and function, as well as an essential factor for proper organ development and metabolism. Indeed, a deregulation of the mechanotransduction process consequent to gene mutations or altered functions of proteins involved in perceiving cellular and extracellular mechanics can lead to a broad range of diseases, from muscular dystrophies and cardiomyopathies to cancer development and metastatization. Here, we recapitulate the involvement of focal adhesion kinase (FAK) in the cellular conditions deriving from altered mechanotransduction processes.
DFT Study on the Mechanism of Iron-Catalyzed Diazocarbonylation

The mechanism of the carbonylation of diazomethane in the presence of iron–carbonyl–phosphine catalysts has been investigated by means of DFT calculations at the M06/def-TZVP//B97D3/def2-TZVP level of theory, in combination with the SMD solvation method. The reaction rate is determined by the formation of the coordinatively unsaturated doublet-state Fe(CO)3(P) precursor followed by the diazoalkane coordination and the N2 extrusion. The free energy of activation is predicted to be 18.5 and 28.2 kcal/mol for the PF3 and PPh3 containing systems, respectively. Thus, in the presence of less basic P-donor ligands with stronger π-acceptor properties, a significant increase in the reaction rate can be expected. According to energy decomposition analysis combined with natural orbitals of chemical valence (EDA–NOCV) calculations, diazomethane in the Fe(CO)3(phosphine)(η1-CH2N2) adduct reveals a π-donor–π-acceptor type of coordination.
What Is the Impact of a Context-Specific Training Program for Home-Based Carers? An Evaluation Study

Introduction: In South Africa (SA), home-based carers (HBCs) play a crucial role at the community level for non-communicable diseases (NCDs) including diabetes mellitus (DM) public health care. The work of HBCs requires them to be knowledgeable about diabetes, and lack of knowledge has implications on their roles for the provision of health information and dietary advice. HBCs need to be provided with specific training to develop their knowledge and skills necessary to enable them to provide care to people with diabetes (PWD) because organizations need to benefit from a pool of well-trained HBCs. Therefore, a training program was developed to improve care for chronic conditions based on local needs. Aim: To implement and evaluate the training program for the HBCs for PWD in Ga-Dikgale village. Methods: HBCs working at Ga- Dikgale villages in four clinics—namely, Dikgale, Seobi Dikgale, Sebayeng, and Makotopong—participated voluntarily. Fifty-five (55) HBCs who attended the training program completed satisfaction survey tools, and furthermore, 45 HBCs completed both pre-training and post-training knowledge questionnaires. Training divided into two sessions which each lasted for two days was conducted. Satisfaction with the training, improvement of knowledge, and perceived impact on daily practice were evaluated using both qualitative and quantitative approaches. Results: Quantitative results indicate that 72% had poor knowledge of pre-training and only 9% post-training. They scored more in a post-test with the following differences: Post-test (mean = 6.00, SD = 1.26); pre-test (mean = 3.31, SD = 1.77). The t-test results indicated the difference to be significant, t = −9.241, p = 0.000. From the qualitative results, the themes that emerged during data analysis from group discussions were HBCs’ achievements from the training, challenges related to diabetes and diet, and suggestions for further training. Conclusions: A context-specific training increased diabetes knowledge among the HBCs for PWD. The results highlighted the importance of training in improving the knowledge of HBCs about the care of PWD. The improvement in diabetes knowledge among HBCs needs to be maintained and sustained to achieve major health benefits for PWD.
Current Advances in 3D Bioprinting Technology and Its Applications for Tissue Engineering

Three-dimensional (3D) bioprinting technology has emerged as a powerful biofabrication platform for tissue engineering because of its ability to engineer living cells and biomaterial-based 3D objects. Over the last few decades, droplet-based, extrusion-based, and laser-assisted bioprinters have been developed to fulfill certain requirements in terms of resolution, cell viability, cell density, etc. Simultaneously, various bio-inks based on natural–synthetic biomaterials have been developed and applied for successful tissue regeneration. To engineer more realistic artificial tissues/organs, mixtures of bio-inks with various recipes have also been developed. Taken together, this review describes the fundamental characteristics of the existing bioprinters and bio-inks that have been currently developed, followed by their advantages and disadvantages. Finally, various tissue engineering applications using 3D bioprinting are briefly introduced.
Study on Electrical Performance of a U-Type Microfluidic Acceleration Switch Using Salt Solution as the Sensitive Electrode

The threshold of microfluidic inertial switch is excessively dependent on the size of the passive valve structure and the gas–liquid surface energy of working liquid. How to achieve high threshold and anti-high overload using liquid with low viscosity and low surface tension is a challenging work. Based on the designed U-type microfluidic inertial switch, the electrical characteristic of salt solution at microscale as well as the threshold and dynamic electrical performance of switch were studied. The VOF and CSD modules in CFD software were employed to analyze the dynamic flow process, and then the air–liquid surface moving displacement curve was compared by the theoretical model. A self-designed acceleration test platform was utilized to measure the static threshold, dynamic threshold, and anti-high overload of the inertial switch. The results show that the U-type microfluidics inertial switch using salt solution as sensitive electrode has better performance in power connection and anti-high overload. In particular, it also has the ability to achieve a range of dynamic threshold by changing the placement of the contact electrode, which can achieve rapid power on and off.