Miracle-dz/UniFAR-FTUs huggingface dataset:
Capital as an Anchor of Economic Activity: Evidence from the 1975 Frost

Does physical capital anchor the spatial distribution of economic activity? If so, how does capital destruction affect local economic activity in the short and the long term? I investigate these questions by examining the 1975 frost that damaged coffee trees in the Brazilian state of Paraná. I find that capital destruction can have persistent effects on the spatial distribution of labor. I identify the effects of capital destruction by comparing the evolution of local economies that had different coffee tree densities before the frost. The frost resulted in a large and persistent displacement of agricultural workers. There was a permanent decline of the local coffee industry after the one-time shock, which reduced labor demand as coffee requires more labor than alternative land uses. The results are consistent with a model in which agglomeration economies within the coffee sector lead to multiple spatial equilibria and in which persistent capital investments keep the spatial distribution of the coffee industry from changing.
On the convergence of two types of estimators of quadratic variation

Recently, two types of innovative estimators of the quadratic variation (QV), namely, RVB and RVQ estimators, are established in Yu (2020, doi.org/10.1002/mma.6863) for an asset's log‐price process permitting time‐varying spot volatility. Both estimators utilize either higher order or cross‐terms of log‐return and they hence have been demonstrated having better convergence property than existing ones (e.g., the widely known realized volatility). However, the proof of the convergence property of RVB/RVQ is rather cumbersome and algebraically complicated. Besides, imposing the spot volatility to be deterministic function of time in the log‐price process seems to have deviated from reality. This article devotes attention to (1) relaxing the volatility restriction on the semimartingale process of asset's price and (2) substantially shortening the original proofs by alternatively employing two useful lemmas (called high‐order Itô isometry and adjoint Itô identity) developed in this study.
A Social Policy Theory of Everyday Borrowing. On the Role of Welfare States and Credit Regimes

Debt has become an essential part of many people's daily lives. This paper develops a new comparative political economy perspective on the relationship between welfare states and household borrowing. I argue that the ways in which welfare states distribute benefits and credit regimes provide access to credit affect how individuals address social risks and, as a consequence, shape patterns of indebtedness. Permissive credit regimes substitute for social policies in limited welfare states, pushing economically disadvantaged groups into debt. Alternatively, credit markets complement social policies in the provision of financial liquidity in comprehensive welfare states, protecting vulnerable groups through government benefits while allowing less-protected affluent groups to borrow money. In restrictive regimes, people instead rely on savings, expenditure cuts, and family support. I test these arguments using an original measure of credit regime permissiveness, cross-national survey data, and full-population administrative records from Denmark and panel data from the United States.
Detrimental effects of lipopolysaccharide on the attachment and outgrowth of various trophoblastic spheroids on human endometrial epithelial cells

Objective Lipopolysaccharide (LPS) from Gram-negative bacteria causes poor uterine receptivity by inducing excessive inflammation at the maternal-fetal interface. This study aimed to investigate the detrimental effects of LPS on the attachment and outgrowth of various types of trophoblastic spheroids on endometrial epithelial cells (ECC-1 cells) in an in vitro model of implantation. Methods Three types of spheroids with JAr, JEG-3, and JAr mixed JEG-3 (JmJ) cells were used to evaluate the effect of LPS on early implantation events. ECC-1 cells were treated with LPS to mimic endometrial infection, and the expression of inflammatory cytokines and adhesion molecules was analyzed by quantitative real-time polymerase chain reaction and western blotting. The attachment rates and outgrowth areas were evaluated in the various trophoblastic spheroids and ECC-1 cells treated with LPS. Results LPS treatment significantly increased the mRNA expression of inflammatory cytokines (CXCL1, IL-8, and IL-33) and decreased the protein expression of adhesion molecules (ITGβ3 and ITGβ5) in ECC-1 cells. The attachment rates of JAr and JmJ spheroids on ECC-1 cells significantly decreased after treating the ECC-1 cells with 1 and 10 μg/mL LPS. In the outgrowth assay, JAr spheroids did not show any outgrowth areas. However, the outgrowth areas of JEG-3 spheroids were similar regardless of LPS treatment. LPS treatment of JmJ spheroids significantly decreased the outgrowth area after 72 hours of coincubation. Conclusion An in vitro implantation model using novel JmJ spheroids was established, and the inhibitory effects of LPS on ECC-1 endometrial epithelial cells were confirmed in the early implantation process.
Ginkgolide B-mediated therapeutic effects on perioperative neurocognitive dysfunction are associated with the inhibition of iNOS-mediated production of NO

Perioperative neurocognitive dysfunction (PND) is a prevalent neurological complication after anesthesia and surgery. Ginkgolide B (GB) has been suggested to improve lipopolysaccharide-induced learning and memory impairment. The present study aimed to investigate whether GB serves a protective role against PND by inhibiting inducible nitric oxide synthase (iNOS) and nitric oxide (NO). Abdominal surgery was performed on 10- to 12-week-old male C57BL/6 mice under isoflurane anesthesia. Prior to surgery, 1400W (a specific iNOS inhibitor) and GB were administered via intraperitoneal injection. Open field and fear conditioning tests were conducted to assess cognitive function on postoperative days 1 and 3. Biochemical assays were performed to evaluate alterations in NO, malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Western blotting was performed to measure iNOS expression in the hippocampus on postoperative day 1. In addition, hematoxylin and eosin staining was performed to detect the neuronal morphology in the hippocampus. Following treatment with 1400W or GB, surgery-induced cognitive dysfunction was improved. Compared with the control group, the surgery group exhibited significant overproduction of iNOS and MDA in the hippocampus on postoperative day 1. Higher levels of NO were also detected in the hippocampus and prefrontal cortex of the surgery group on postoperative day 1. Furthermore, pretreatment with 1400W or GB significantly inhibited the surgery-induced elevation of NO and MDA in brain tissues. Moreover, GB pretreatment significantly inhibited surgery-induced downregulation of SOD and upregulation of iNOS. Surgery-induced increases in neuronal loss and the Bax/Bcl-2 ratio in the hippocampus were significantly inhibited by pretreatment with GB. Collectively, the results of the present study demonstrated that the therapeutic effects of GB on PND were associated with inhibition of iNOS-induced NO production, increased SOD, and the alleviation of neuronal loss and apoptosis.
The Nature of the Silicon–Oxygen Bond

Even though siloxanes form the basis for the entire worldwide silicone industry, the nature of silicon–oxygen bonding has not been well understood. In the present work we employ correlated and highly polarized basis levels and natural bond orbital techniques to analyze the anomalous structural and basicity properties of inorganic Si–O–Si linkages compared to organic C–O–C congeners. We find that vicinal hyperconjugative interactions of nO → σ*XH type are primarily responsible for promoting the increased torsional flexibility and reduced basicity that strongly distinguish disiloxanes from ethers. Paradoxically, the key to enhanced siloxane hyperconjugation lies in the inherently larger X–O–X bond angle for X = Si (consistent with electronegativity and hybridization variations predicted by Bent’s rule), even though such resonance-type corrections are generally stronger in ethers (X = C) at any particular X–O–X angle. These findings have important implications for many aspects of silicone chemistry as well a.
Impact of ecotourism on abundance, diversity and activity patterns of medium-large terrestrial mammals at Brownsberg Nature Park, Suriname

The impacts of ecotourism on biodiversity are poorly understood and the outcome of this type of research is often contradictory. On the one hand ecotourism could impact the occurrence, survival or behavior of species, on the other hand ecotourism is often mentioned as providing a “human shield” by deterring negative practices like gold mining, logging and hunting. Brownsberg Nature Park is easily the most visited protected area of Suriname, with a high number of ecotourists visiting from abroad. A four-year study on the impact of ecotourism on medium-large terrestrial mammals was carried out between 2013 and 2016 using 16 camera trap stations. The area has a clear gradient of tourism pressure, with the pressure decreasing further away from the lodging facilities. Evidently, the impacts of human presence on the mammal communities were more significant in the busiest areas. Most species avoided areas with many hikers or switched to a more nocturnal activity pattern. In these areas the impact was not reflected in species numbers, however it was causing a significant decrease in the diversity of mammals. On the other hand, vehicles had little impact on species avoidance or diversity, but did increase nocturnality, even more than hikers. A few species seemed to be "attracted" by hikers and/or traffic. Giant armadillos (Priodontes maximus) and spotted pacas (Cuniculus paca) used the pools in the road created by traffic. Ocelots (Leopardus pardalis), margays (Leopardus wiedii) and red-rumped agoutis (Dasyprocta leporina) seemed to favor human disturbance probably because of predator release. Some of the most impacted species were the jaguar (Panthera onca), puma (Puma concolor) and lowland tapir (Tapirus terrestris), all three species with significant contribution to ecosystem balance. Management measures should focus on lowering the number of hikers in popular places and limiting the number of vehicles in recreational areas.
Numerical abundance and biomass reveal different temporal trends of functional diversity change in tropical fish assemblages.

Understanding how the biodiversity of freshwater fish assemblages changes over time is an important challenge. Until recently most emphasis has been on taxonomic diversity but it is now clear that measures of functional diversity can shed new light on the mechanisms that underpin this temporal change. Fish biologists use different currencies, such as numerical abundance and biomass, to measure the abundance of fish species. However, because they are not necessarily equivalent, these alternative currencies have the potential to reveal different insights into trends of functional diversity in natural assemblages. Here we asked how conclusions about temporal trends in functional diversity are influenced by the way in which the abundance of species has been quantified. To do this we computed two informative metrics, for each currency, for 16 freshwater fish assemblages in Trinidad's Northern Range that had been surveyed repeatedly over five years. We found that numerical abundance and biomass uncover different directional trends in these assemblages for each facet of functional diversity, and as such inform hypotheses about the ways in which these systems are being restructured. On the basis of these results we concluded that a combined approach, in which both currencies are employed, contributes to our understanding of the ecological processes that are involved in biodiversity change in freshwater fish assemblages. This article is protected by copyright. All rights reserved.
Effects of a period without mandatory physical training on maximum oxygen uptake and anthropometric parameters in naval cadets

The effects of a period without physical training on the civilian population are well established. However, no studies show the effects of a period without mandatory physical training on maximum oxygen uptake (VO2 max) and anthropometric parameters in naval cadets. This study aimed to investigate changes in VO2 max and anthropometric parameters after 12 weeks without mandatory physical training in naval cadets. The sample was 38 healthy and physically active naval cadets. The measured variables, including VO2 max and anthropometric parameters, were evaluated through the 12-minute race test (12MRT) and the somatotype. Both variables had a separation of 12 weeks without mandatory physical training. A t-test for related samples was used to evidence changes between the test and post-test; effect size was calculated through Cohen’s d-test. Distance in 12MRT and VO2 max showed significant decreases at the end of 12 weeks without mandatory physical training (p < 0.001). Likewise, the tricipital skinfold thickness and the endomorphic component showed significant increases (p < 0.05). 12 weeks without mandatory physical training significantly reduces the VO2 max in naval cadets. Simultaneously, the same period without physical training increases both the tricipital skinfold thickness and the endomorphic component in this population.
Evaluation of self-report screening measures in the detection of depressive and anxiety disorders among children and adolescents with systemic lupus erythematosus

Background There are no validated screening measures for depressive or anxiety disorders in childhood Systemic Lupus Erythematosus (cSLE). We investigated cross-sectionally (1) the prevalence of depressive and anxiety disorder in cSLE. (2) the validity of the Centre for Epidemiologic Studies Depression Scale for Children (CES-DC) and the Screen for Childhood Anxiety and Related Disorders (SCARED) measures in identifyingthese disorders. Methods Participants 8-18 years with cSLE/incipient cSLE completed CES-DC, SCARED, and Quality OfMy Life (QOML) measures. Parents completed the SCARED-Parent measure. Diagnosis was by gold-standard psychiatric interview and determined prevalence of psychiatric disorder. Receiver Operating Characteristics Area under the Curve (ROCAUC) evaluated screening measure diagnostic performance. Results Ofseventy-two parent-child dyads, 56 interviews were completed. Mean screen scores were: CES-DC = 15 (range 1-49, SD 12), SCARED-C = 22 (range 2-61, SD 14), SCARED-P = 13 (range 0-36, SD 8). Depressive disorder screen positivity (CES-DC ≥ 15) was 35% (vs. prevalence 5%). Anxiety disorder screen positivity (SCARED ≥ 25) was 39% (vs. prevalence 16%). CES-DC ROCAUC = 0.98 and SCARED-C ROCAUC = 0.7 (cut-points 38 and 32 respectively). Conclusions Diagnostic thresholds for depressive and anxiety disorderscreening measures are high for both CES-DC and SCARED-C in cSLE. Brief focused interview should follow to determine whether psychiatric evaluation is warranted.
Glucosylceramide and galactosylceramide, small glycosphingolipids with significant impact on health and disease

Abstract Numerous clinical observations and exploitation of cellular and animal models indicate that glucosylceramide (GlcCer) and galactosylceramide (GalCer) are involved in many physiological and pathological phenomena. In many cases, the biological importance of these monohexosylcermides has been shown indirectly as the result of studies on enzymes involved in their synthesis and degradation. Under physiological conditions, GalCer plays a key role in the maintenance of proper structure and stability of myelin and differentiation of oligodendrocytes. On the other hand, GlcCer is necessary for the proper functions of epidermis. Such an important lysosomal storage disease as Gaucher disease (GD) and a neurodegenerative disorder as Parkinson’s disease are characterized by mutations in the GBA1 gene, decreased activity of lysosomal GBA1 glucosylceramidase and accumulation of GlcCer. In contrast, another lysosomal disease, Krabbe disease, is associated with mutations in the GALC gene, resulting in deficiency or decreased activity of lysosomal galactosylceramidase and accumulation of GalCer and galactosylsphingosine. Little is known about the role of both monohexosylceramides in tumor progression; however, numerous studies indicate that GlcCer and GalCer play important roles in the development of multidrug-resistance by cancer cells. It was shown that GlcCer is able to provoke immune reaction and acts as a self-antigen in GD. On the other hand, GalCer was recognized as an important cellular receptor for HIV-1. Altogether, these two molecules are excellent examples of how slight differences in chemical composition and molecular conformation contribute to profound differences in their physicochemical properties and biological functions.
Efficacy and safety of azithromycin in Covid‐19 patients: A systematic review and meta‐analysis of randomized clinical trials

Azithromycin (AZM) is commonly used in Covid‐19 patients based on low‐quality evidence, increasing the risk of developing adverse events and antimicrobial resistance. The current systematic review and meta‐analysis investigated the safety and efficacy of AZM in treating Covid‐19 patients using published randomized controlled trials. Google Scholar, PubMed, Scopus, Cochrane Library, Clinical Trials.gov, MEDLINE, bioRxiv and medRxiv were searched for relevant studies. The random‐effects model was used to pool estimates using the Paule–Mandel estimate for heterogeneity. The odds ratio and raw difference in medians were used for dichotomous and continuous outcomes, respectively. The analysis included seven studies with 8822 patients (median age, 55.8 years; 61% males). The risk of bias was assessed as ‘low’ for five of the seven mortality results and as ‘some concerns’ and ‘high’ in one trial each. There were 657/3100 (21.2%) and 1244/5654 (22%) deaths among patients randomized to AZM and standard of care, respectively. The use of AZM was not associated with mortality in Covid‐19 patients (OR = 0.96, 95% CI 0.88–1.05, p = 0.317 based on the random‐effect meta‐analysis). The use of AZM was not associated with need for invasive mechanical ventilation (OR = 0.96, 95% CI 0.49–1.87, p = 0.85) and length of stay (Δ = 1.11, 95% CI −2.08 to 4.31, p = 0.49). The results show that using AZM as routine therapy in Covid‐19 patients is not justified due to lack of efficacy and potential risk of bacterial resistance that is not met by an increased clinical benefit.
Comment on Trophic strategy and bleaching resistance in reef-building corals

We show how to foresee and account for risks in applying the SIBER framework to coral trophic ecology. In an era of major environmental changes, understanding corals’ resistance to bleaching is as crucial as it is challenging. A promising framework for inferring corals’ trophic strategies from Stable Isotope Bayesian Ellipses has been recently proposed to this end. As a contribution to this framework, we quantify a risk of bias inherent in its application and propose three alternative adjustments.
Directional atherectomy before paclitaxel coated balloon angioplasty in complex femoropopliteal disease: The VIVA REALITY study

Drug coated balloon (DCB) angioplasty significantly reduces reintervention rates in patients with symptomatic femoropopliteal peripheral artery disease (PAD). However, stand‐alone DCB use in long, severely calcified lesions is frequently associated with vessel recoil and/or high‐grade dissections necessitating provisional stent implantation.
A novel substrate for arrhythmias in Chagas disease

Background Chagas disease (CD) is a neglected disease that induces heart failure and arrhythmias in approximately 30% of patients during the chronic phase of the disease. Despite major efforts to understand the cellular pathophysiology of CD there are still relevant open questions to be addressed. In the present investigation we aimed to evaluate the contribution of the Na+/Ca2+ exchanger (NCX) in the electrical remodeling of isolated cardiomyocytes from an experimental murine model of chronic CD. Methodology/Principal findings Male C57BL/6 mice were infected with Colombian strain of Trypanosoma cruzi. Experiments were conducted in isolated left ventricular cardiomyocytes from mice 180–200 days post-infection and with age-matched controls. Whole-cell patch-clamp technique was used to measure cellular excitability and Real-time PCR for parasite detection. In current-clamp experiments, we found that action potential (AP) repolarization was prolonged in cardiomyocytes from chagasic mice paced at 0.2 and 1 Hz. After-depolarizations, both subthreshold and with spontaneous APs events, were more evident in the chronic phase of experimental CD. In voltage-clamp experiments, pause-induced spontaneous activity with the presence of diastolic transient inward current was enhanced in chagasic cardiomyocytes. AP waveform disturbances and diastolic transient inward current were largely attenuated in chagasic cardiomyocytes exposed to Ni2+ or SEA0400. Conclusions/Significance The present study is the first to describe NCX as a cellular arrhythmogenic substrate in chagasic cardiomyocytes. Our data suggest that NCX could be relevant to further understanding of arrhythmogenesis in the chronic phase of experimental CD and blocking NCX may be a new therapeutic strategy to treat arrhythmias in this condition.
Sexual Role Identity and Anal Sex Positioning among Brazilian, Colombian, and Dominican Immigrant Sexual Minority Men

ABSTRACT Sexual role identities for anal sex and anal sex positionings are often conflated in research. This study aimed to identify profiles of sexual minority men based on sexual role identity and anal sex positioning and determine correlates of these profiles, including individual (i.e., HIV-status identity, condom use, partner type, sexual orientation) and cultural (i.e., same-race partnering, country of birth, acculturation) factors. A sample of Brazilian, Colombian, and Dominican immigrant sexual minority men (n = 480) living in New York City were surveyed about identity and positioning at the two most recent encounters. Individual and cultural factors were examined as correlates of profiles identified through latent class analysis. Four latent classes were identified: No Labels (12%), Receptive Bottoms (16%), Insertive Tops (23%), Insertive and Receptive Versatiles (49%). We found a concordance rate of ~70% between identity and behavior. A proportion (12%) of behaviorally versatile men chose not to use sexual role labels. Living with HIV was associated with receptive and versatile classes, sex with main partners was associated with versatility, and sexual orientation differed across classes. Identity and behavior should not be used interchangeably in research or applied domains, particularly given the complexities of versatility and men who prefer to not use labels.