Miracle-dz/UniFAR-FTUs huggingface dataset:
Testing small‐scale ecological gradients and intraspecific differentiation for hundreds of kelp forest species using haplotypes from metabarcoding

DNA metabarcoding has been increasingly used to detail distributions of hundreds of species. Most analyses focus on creating molecular operational taxonomic units (MOTUs) from complex mixtures of DNA sequences, but much less common is use of the sequence diversity within these MOTUs. Here we use the diversity of COI haplotypes within MOTUs from a California kelp forest to infer patterns of population abundance, dispersal and population history from 527 species of animals and algae from 106 samples of benthic habitats in Monterey Bay. Using haplotypes as a unit we show fine‐grained differences of abundance across locations for 15 species, and marked aggregation from sample to sample for most of the common species of plants and animals. Previous analyses could not distinguish these patterns from artefacts of amplification or sequence bias. Our haplotype data also reveal strong population genetic differentiation over small spatial scales for 48 species of red algae, sponges and Bryozoa. Last, phylogenetic analysis of mismatch frequencies among haplotypes show a wide variety of demographic histories from recent expansions to long, stable population sizes. These analyses show that abundant, small‐bodied marine species that are often overlooked in ecological surveys can have strikingly different patterns of ecological and genetic structure leading to population, ecological and perhaps adaptive differences between habitats. MOTU diversity data from the same sequencing efforts that generate species‐level analyses can greatly increase the scope and value of metabarcoding studies.
Water‐Soluble Ligands, Metal Complexes, and Catalysts: Synergism of Homogeneous and Heterogeneous Catalysis

Rapid developments in the field of catalysis are leading to an increased demand for tailor-made catalysts. Water-soluble complex catalysts, which are being intensively investigated at the present time, combine the advantages of homogeneous and heterogeneous catalysis: simple and complete separation of the product from the catalyst, high activity, and high selectivity. From the large number of available water-soluble ligands, the appropriate catalysts can be developed for many reactions. The industrial applications in the fields of hydrogenation and hydroformylation have already indicated the wide scope of this type of catalyst. In addition, the annual production of 300 000 tons of butyraldehyde through application of water-soluble rhodium complexes at Hoechst AG in Oberhausen, Germany, has demonstrated the industrial importance of the concept of complex-catalyzed reactions in aqueous two-phase systems. The efficient operation of catalytic processes increasingly requires the loss-free recycling of the noble metal catalyst, and this can be simply and economically realized in two-phase systems. Special applications in biochemical problems open up developments in the field of water-soluble transition metal complexes that far transcend the familiar kinds of homogeneous catalysis. In the near future, the investigation and application of metal complex catalysts that are compatible with the physiological, cheap, and environmentally friendly solvent, water, is likely to become a high priority in catalysis research.
A comprehensive in silico and in vitro studies on quinizarin: a promising phytochemical derived from Rhizophora mucronata Lam

Abstract Mangrove plants are a great source of phytomedicines, since from the beginning of human civilization and the origin of traditional medicines. In the present study, ten different mangrove leaf methanolic extracts were screened for the type of phytochemicals followed by assessing antimicrobial, anti-oxidant and anti-cancer activities. The efficient methanolic crude extract of Rhizospora mucornata was further purified and characterized for the presence of the bioactive compound. Based on UV-visible spectroscopy, FTIR, NMR and HRMS analysis, the bioactive compound was 1,4-dihydroanthraquinone; also termed as Quinizarin. This identified compound was potential in exhibiting antimicrobial, antioxidant, and cytotoxic activity. Quinizarin inhibited the growth of Bacillus cereus and Klebsiella aerogenes with minimum inhibitory concentration (MIC) of 0.78 and 1.5 mg/ml. The DPPH free radical scavenging assay revealed the maximum activity of 99.8% at the concentration of 200 µg/ml with an IC50 value of 12.67 ± 0.41 µg/ml. Cytotoxic assay against HeLa (cervical) and MDA-MB231(breast) cancer cell lines revealed IC50 values to be 4.60 ± 0.26 and 3.89 ± 0.15 µg/ml. Together the results of molecular docking and molecular dynamics simulation studies explained that Quinizarin molecule showed stronger binding affinity (−6.2 kcal/mol) and significant structural stability towards anti-apoptotic Bcl-2 protein. Thus, the study put forth the promising role of the natural molecule - Quinizarin isolated from R. mucornata in the formulation of therapeutic drugs against bacterial infections and cancer. Communicated by Ramaswamy H. Sarma
Circulating miR-145 as a marker of therapeutic response to anti-TNF therapy in patients with ankylosing spondylitis.

Circulating miRNAs appear promising therapeutic and prognostic biomarkers. We aimed to investigate the predictive value of circulating miRNAs on the disease outcome following anti-TNF therapy in patients with ankylosing spondylitis (AS). Our study included 19 AS patients assessed at baseline (M0), after three (M3) and twelve months (M12) of therapy. Total RNA was isolated from plasma. A comprehensive analysis of 380 miRNAs using TaqMan Low Density Array (TLDA) was followed by a single assay validation of selected miRNAs. All AS patients had high baseline disease activity and an excellent response to anti-TNF therapy at M3 and M12. TLDA analysis revealed the dysregulation of 17 circulating miRNAs, including miR-145. Single assay validation confirmed that miR-145 is significantly downregulated at M3 compared to baseline. The decrease in the levels of miR-145 from M0 to M3 negatively correlated with the change in BASDAI from M0 to M3; however and positively correlated with disease activity improvement from M3 to M12 as per BASDAI and ASDAS. The predictive value of the early change in miR-145 and levels of miR-145 at M3 was further validated by Receiver operating curves analysis. We show that the early change in circulating miR-145 may be a predictor for the future outcome of AS patients treated with TNF inhibitors. Patients with a more significant decrease in miR-145 levels may show further significant improvement of disease activity after 12 months. Monitoring the expression of miR-145 in plasma in AS patients may, therefore, influence our therapeutic decision-making.
Characteristics of Women Presenting at the Emergency Department Who Choose Not to Disclose Being Subjected to Intimate Partner Violence

Literature suggests that some women refrain from disclosing being subjected to intimate partner violence (IPV) upon their contact with the health care system. Such disclosure becomes critical when the violence compels women to seek urgent medical care. The purpose of the present study was to compare characteristics of women who disclose and women who do not disclose being subjected to domestic violence, when presenting at the Emergency Department (ED). In this chart review study, characteristics of 56 randomly sampled women who disclosed and 50 who did not disclose being subjected to domestic violence, when presenting at the ED in a medical center located in central-northern Israel between 2015 and 2018, were compared. It was found that women who did not disclose were more likely to be pregnant, legally defined as helpless, and dependent on others to some extent. In addition, they were more likely to be hospitalized, which may indicate a more severe injury, and were more likely to have been subjected to psychological abuse or neglect alone. A trauma informed approach should guide specific interventions with a focus on women with these characteristics in the ED, in order to facilitate their disclosure of IPV.
Extracellular Vesicles: Novel Roles in Neurological Disorders

Exosomes are small extracellular vesicles (EVs) secreted by almost all cells, which have been recognized as a novel platform for intercellular communication in the central nervous system (CNS). Exosomes are capable of transferring proteins, nucleic acids, lipids, and metabolites between neurons and glial cells, contributing to CNS development and maintenance of homeostasis. Evidence shows that exosomes originating from CNS cells act as suppressors or promoters in the initiation and progression of neurological disorders. Moreover, these exosomes have been shown to transfer molecules associated with diseases through the blood-brain barrier (BBB) and thus can be detected in blood. This unique feature enables exosomes to act as potential diagnostic biomarkers for neurological disorders. In addition, a substantial number of researches have indicated that exosomes derived from mesenchymal stem cells (MSCs) have repair effects on neurological disorders. Herein, we briefly introduce the roles of exosomes under physiological and pathological conditions. In particular, novel roles of exosomes as potential diagnostic biomarkers and therapeutic tools for neurological disorders are highlighted.
Effect of a Compassion Cultivation Training Program for Caregivers of People With Mental Illness in Denmark

Key Points Question Is a compassion cultivation training (CCT) intervention effective in decreasing psychological distress in informal caregivers of people with mental illness? Findings In this randomized clinical trial including 161 caregivers randomized to a CCT program or waitlist group, caregivers who received CCT experienced significant improvements in depression, anxiety, and stress, and the improvements were maintained at 6-month follow-up. Meaning Theses findings suggest that the CCT intervention was effective in decreasing psychological distress in caregivers of people with mental illness.
Chemovariations in Hypenia essential oils under infrageneric and phenological influences

Abstract The essential oils (EOs) of six Hypenia species that are endemic in the central Brazilian Cerrado were assessed by GC/MS and chemometric analysis. EO variations according to two taxonomic sections, combined with two phenophases (vegetative and reproductive), showed that 23.2% of total variance was explained by such predictors. Variation partitioning showed that the largest pure contribution was attributed to section (43.7%), followed by phenophase (39.3%), with 17% of joint influence. A multivariate regression tree (MRT) indicated that 13 EO constituents may be potential chemomarkers for distinguishing sections and two developmental stages in the genus. Graphical Abstract
Soil priming effect and its responses to nutrient addition along a tropical forest elevation gradient

Priming plays important roles in terrestrial carbon cycling, but the patterns and drivers of priming and its responses to nutrient addition in tropical forests remain unclear. By collecting soils along a tropical forest elevation gradient, we conducted an incubation experiment with 13C‐labeled glucose and nutrient (N and/or P) additions. Results showed that priming effects increased soil organic matter decomposition by 44 ± 12% across elevations, and priming intensity decreased significantly with elevation. Among soil and microbial properties, soil organic carbon (SOC) content and pH were two key factors negatively and positively regulating priming, respectively. Across elevations, the additions of N, P, or both of them (NP) did not significantly change priming. However, the variations in the effects of nutrient (N and/or P) addition on priming significantly correlated with initial soil nutrient (N or P) availability. The intensity for the effects of N addition on priming decreased significantly with initial soil N availability, and that for the effects of P and NP addition on priming decreased with initial soil P availability. Based on these relationships, we proposed a conceptual framework linking stoichiometric decomposition and nutrient mining hypotheses, in which the former dominates in low‐nutrient availability soils and the latter dominates in high‐nutrient availability soils. This conceptual framework can help to explain the contrasting effects of nutrient addition on priming. Collectively, our findings highlight the roles of SOC content and soil pH in regulating priming intensity, and the role of initial soil nutrient availability in regulating the effects of nutrient addition on priming.
Identity cues influence sexual minorities’ anticipated treatment and disclosure intentions in healthcare settings: Exploring a multiple pathway model

The present work experimentally examines how identity cues that signal minority inclusion contribute to sexual minorities’ (SM) healthcare visit expectations. We find that minority representation cues reduced SM’s (N = 188) expectations of a healthcare provider’s bias and increased perceived provider cultural competency which was, in turn, associated with lower anticipated identity-based devaluation and greater sexual orientation disclosure comfort. Providers’ diversity-valuing statements had mixed effects highlighting the importance of more concrete indicators of inclusion in this context. This work suggests that a lack of identity safety cues in healthcare settings may contribute to disparate health outcomes for sexual minority populations.
Improved extraction of vanadium from a Chinese vanadium‐bearing stone coal using a modified roast‐leach process

Vanadium is a rare metal mainly used in the steel industry. Vanadium-bearing stone coal is an important source of vanadium in China. The common process used to extract vanadium from the stone coals is roast-leach, which has the disadvantages of low vanadium recovery and significant pollution. This work presents a modified roast-leach process in order to improve the efficiency of the extraction. The modifications include a two-step leaching process: first water leach, followed by an acid leach of the water-leaching residue in a dilute HCl solution. In addition, the precipitate was recycled from the acid-leaching solution to the oxidation roast for use as a roasting agent, and low dosages of sodium salts (Na2CO3 and Na2SO4) were included in the oxidation roast to reduce the volume of HCl and Cl2 exhaust gases and environmental pollution. The study was performed on a typical vanadium-bearing stone coal from the Xiushui mine in Jiangxi province, China. The results show that the modified process achieved a vanadium recovery of 85% from the stone coal, much higher than that from a one-step leach process (water leach or acid leach alone). Also, it has been found that optimum values of the roast temperature and time in the preroast (750 °C and 30 min) and oxidation roast (780 °C and 90 min) were required in order to obtain the maximum vanadium recovery from stone coal. It is shown that the modified roast-leach process is an economic and effective process for the recovery of vanadium from stone coals. Copyright © 2009 Curtin University of Technology and John Wiley & Sons, Ltd.
Coarse Aerosol Concentration Over the North Polar Waters of the Atlantic

This paper presents the data obtained during the polar research cruises of the r/v Oceania in 1995–2001. The aerosol size distributions were measured using the laser particle counter CSASP-100-HV-SP. The size spectral density between diameters 0.5–15 μ m was found to fit the following form: dN/dr = n(2 r) = exp (a2 r + b) , where: a = − 0.29 and b are functions of wind speed. Additionally, poor precision of the sea spray emission determination using only the aerosol concentration data measured at one altitude was confirmed.
Psychometric analysis of the behavioral assessment screening tool (BAST) in adults with stroke

ABSTRACT Purpose The purpose of this study was to examine the initial psychometric properties of the Behavioral Assessment Screening Tool (BAST), a self-reported measure of neurobehavioral symptoms, in adults with stroke. Methods We assessed subscale and item-level reliabilities of the five BAST subscales in 75 community-dwelling adults with stroke. We further assessed the known-groups validity of the BAST to differentiate individuals with and without self-reported lifetime stroke history (n = 47 with stroke and n = 1843 neurologically healthy controls). Results Cronbach’s alpha coefficients of all subscales were >0.7, demonstrating acceptable to good internal consistency reliabilities, and corrected item-total correlations were all >. 30 demonstrating good item-level reliabilities. ROC curves demonstrated strong known-groups validity of the negative affect, executive function, and fatigue subscales for classifying stroke versus healthy controls (AUC = .669-.758, p < .001). Conclusion The BAST demonstrates good initial psychometric properties as a screening tool to identify neurobehavioral symptoms in community-dwelling adults with stroke. Future work will add stroke-specific items, further assess the validity of the BAST, and employ item response theory or Rasch analyses to identify highly discriminative items for potential smart device-based ecological momentary assessments.
Comparisons of atomic thermal motions for graphite at 300 K based on X-ray, neutron, and phonon-spectrum data

The mean-square amplitudes of vibration in graphite based on an X-ray charge-density analysis are 0.0032 (2) and 0.0140 (3) A2 parallel to and perpendicular to the basal plane, respectively. Values for the parallel vibrations of 0.0031 (6) and 0.0032 A2 were derived from temperature-dependent neutron measurements and a calculated phonon spectrum. The neutron measurements and the phonon spectrum both predict lower values [0.0090 (20) and 0.0098 A2] for the out-of-plane vibrations. This small discrepancy may be caused by small changes in the core atomic scattering factors from the free-atom values or by a deficiency in the phonon-spectrum model.
Association of Umbilical Cord Management Strategies With Outcomes of Preterm Infants: A Systematic Review and Network Meta-analysis.

Importance It is unclear which umbilical cord management strategy is the best for preventing mortality and morbidities in preterm infants. Objective To systematically review and conduct a network meta-analysis comparing 4 umbilical cord management strategies for preterm infants: immediate umbilical cord clamping (ICC), delayed umbilical cord clamping (DCC), umbilical cord milking (UCM), and UCM and DCC. Data Sources PubMed, Embase, CINAHL, and Cochrane CENTRAL databases were searched from inception until September 11, 2020. Study Selection Randomized clinical trials comparing different umbilical cord management strategies for preterm infants were included. Data Extraction and Synthesis Data were extracted for bayesian random-effects meta-analysis to estimate the relative treatment effects (odds ratios [OR] and 95% credible intervals [CrI]) and surface under the cumulative ranking curve values. Main Outcomes and Measures The primary outcome was predischarge mortality. The secondary outcomes were intraventricular hemorrhage, severe intraventricular hemorrhage, need for packed red blood cell transfusion, and other neonatal morbidities. Confidence in network meta-analysis software was used to assess the quality of evidence and grade outcomes. Results Fifty-six studies enrolled 6852 preterm infants. Compared with ICC, DCC was associated with lower odds of mortality (22 trials, 3083 participants; 7.6% vs 5.0%; OR, 0.64; 95% CrI, 0.39-0.99), intraventricular hemorrhage (25 trials, 3316 participants; 17.8% vs 15.4%; OR, 0.73; 95% CrI, 0.54-0.97), and need for packed red blood cell transfusion (18 trials, 2904 participants; 46.9% vs 38.3%; OR, 0.48; 95% CrI, 0.32-0.66). Compared with ICC, UCM was associated with lower odds of intraventricular hemorrhage (10 trials, 645 participants; 22.5% vs 16.2%; OR, 0.58; 95% CrI, 0.38-0.84) and need for packed red blood cell transfusion (9 trials, 688 participants; 47.3% vs 32.3%; OR, 0.36; 95% CrI, 0.23-0.53), with no significant differences for other secondary outcomes. There was no significant difference between UCM and DCC for any outcome. Conclusions and Relevance Compared with ICC, DCC was associated with the lower odds of mortality in preterm infants. Compared with ICC, DCC and UCM were associated with reductions in intraventricular hemorrhage and need for packed red cell transfusion. There was no significant difference between UCM and DCC for any outcome. Further studies directly comparing DCC and UCM are needed.
Using deep learning for pixel-defect corrections in flat-panel radiography imaging

Abstract. Purpose: Flat-panel radiography detectors employ thin-film transistor (TFT) panels to acquire high-quality x-ray images. Pixel defects occur due to circuit shorts or opens in the TFT panel. The defects may degrade the image quality, as well as lower the production yield, and eventually raise the production cost. Hence, it is important to develop an appropriate defect correction algorithm for acquired images. Traditional correction algorithms are based on a complicated adaptive filtering technique, which exploits neighbor pixels, to faithfully preserve the edge components. Because of the complexity of the traditional sophisticated approaches, optimizing their correction performances is difficult. Approach: We considered various pixel-defect correction algorithms based on different deep learning models, such as the artificial neural network (ANN), convolutional neural network (CNN), concatenate CNN, and generative adversarial networks (GAN). We considered two cases of maximal defect sizes, 3 × 3 and 5 × 5 pixels, and conducted extensive learning experiments to find the best structures of the learning models using the mean square error (MSE) as the loss function. Results: To conduct experiments, practical chest x-ray images were acquired from a general radiography detector. The MSE values of the correction results from ANN, CNN, concatenate CNN, and GAN were 69.40, 75.13, 68.21, and 73.77, respectively, and were much smaller than that of the conventional template match correction method. Conclusions: A concatenate CNN showed the best defect-correction performance. However, ANN could achieve a similar correction performance with much smaller encoding complexity. Therefore, the single-layer ANN can efficiently conduct defect corrections in terms of both correction and complexity.
ELECTRONIC CORRELATIONS IN NI 2P AND 3P MAGNETIC-X-RAY DICHROISM AND X-RAY PHOTOEMISSION OF FERROMAGNETIC NICKEL

The magnetic X-ray dichroism (MXD) in X-ray absorption together with the photoemission of the Ni 2p and 3p levels in ferromagnetic nickel have been analysed using an Anderson impurity model taking into account multiplet splitting. Good agreement with experimental results was obtained for a ground state of 18% d8, 49% d9 and 33% d10 character. In the Ni 2p MXD the branching ratio and total intensity are determined by the spin-orbit interaction and orbital momentum of the ground state, and the satellite structure results from transitions to a pd9 final state.
RESORCINOL-FORMALDEHYDE ION EXCHANGE RESIN CHEMISTRY FOR HIGH LEVEL WASTE TREATMENT

A principal goal at the Savannah River Site is to safely dispose of the large volume of liquid nuclear waste held in many storage tanks. In-tank ion exchange technology is being considered for cesium removal using a polymer resin made of resorcinol formaldehyde that has been engineered into microspheres. The waste under study is generally lower in potassium and organic components than Hanford waste; therefore, the resin performance was evaluated with actual dissolved salt waste. The ion exchange performance and resin chemistry results are discussed.