Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessment of Functional Characterization and Comparability of Biotherapeutics: a Review

Abstract. The development of monoclonal antibody (mAb) biosimilars is a complex process. The key to their successful development and commercialization is an in-depth understanding of the key product attributes that impact safety and efficacy and the strategies to control them. Functional assessment of mAb is a crucial part of the comparability of biopharmaceutical drugs. The development of a relevant and robust functional assay requires an interdisciplinary approach and sufficient flexibility to balance regulatory concerns as well as dynamics and variability during the manufacturing process. Although many advanced tools are available to study and compare the potency and bioactivity of the protein, most of these techniques suffer from major shortcomings that limit their routine use. These include the complexity of the task, establishment of the relevance of the chosen method with the mechanism of action (MOA) of the biosimilar, cost and extended time of analysis, and often the ambiguity in interpretation of the resulting data. To overcome or to address these challenges, the use of multiple orthogonal state-of-the-art techniques is a necessary prerequisite.
Cumulative Effective Dose from Medical Imaging in Inflammatory Bowel Disease

Inflammatory bowel diseases (IBD) are chronic and relapsing disorders usually requiring numerous medical imaging. IBD patients might be exposed to a large dose of radiation. As a cumulative effective dose (CED) ≥ 50 mSv is considered significant for stochastic risks of cancer, it is important to monitor the radiation exposure of IBD patients. In the present work, we aimed to quantify the mean CED in IBD patients and identify factors associated with exposure to high doses of diagnostic radiation. A retrospective chart view of patients with IBD hospitalized between 2015 and 2019 was performed. A total of 65 patients with Crohn’s disease (CD) and 98 patients with ulcerative colitis (UC) were selected. Of all imaging studies performed, 73% were with doses of ionizing radiation. Mean CED (SD) amounted to 19.20 (15.64) millisieverts (mSv) and 6.66 (12.39) mSv, respectively, in patients with CD and UC (p < 0.00001). Only 1.84% of the patients received CED ≥ 50 mSv. We identified three factors associated with CED in the IBD patients: number of surgical procedures, and number and length of hospitalization. CD patients with strictures or penetrating disease and UC patients with extensive colitis were more likely to receive higher radiation doses.
Multi-Plate Shift Clutch Morbidity Index Forecasting Method Study

According to the configuration characteristic of high-power transmissions and working principle of multi-plate shift clutch, the clutch dragging process is analyzed at first in this paper. After introducing the usual monitoring method of clutch dragging process, a portable vibration monitoring method is proposed. And then a GMDH forecast method on the basis of the morbidity index is presented. The practical application of the method can reduce the probability of clutch invalidation, and significantly extend the clutch’s working life.
INVESTIGATING THE STABILITY OF ENGINEERED STRUCTURES USING ACOUSTIC VALIDATION OF NUMERICAL MODELS

Understanding the mechanical stability of rock and concrete structures is of utmost importance to the safe and efficient construction and operation of engineering projects. One of the most highlighted cases in recent years is the feasibility study for disposing of radioactive waste in deep underground repositories. Many international organizations are currently investigating this concept using underground laboratories as a tool to provide development and testing of engineering designs, storage methodologies and monitoring techniques. Acoustic techniques allow fracture growth around underground excavations to be observed and allow a quantification of the extent of damage and how this affects the physical properties of the rock. Current work involves integrating physical measurements from these techniques with numerical models to further understand the mechanics of the damage processes. It is proposed to then use these models in the repository design process to predict instabilities in the engineered structures that may occur over the repository's operational lifetime. Acoustic techniques will then provide remote monitoring capabilities that give a feedback mechanism by which these models can be updated and validated. In this paper we demonstrate the integration of acoustic monitoring and numerical modeling and discuss its future applicability to engineered structures in both rock and concrete.
State-of-the-art aluminum-free 980-nm laser diodes

InGaAs/GaInAsP/GaInP ridge waveguide 980-nm laser diodes for pumping light into erbium doped fiber amplifiers are reviewed. These lasers have very good performance characteristics. They exhibit kink-free, single mode emission up to a power of 250 mW with a slope efficiency of 0.7 to 0.95 W/A, a thermally limited maximum power of 450-500 mW, and the threshold current density of about 150 A/cm/sup 2/. They are relatively stable against temperature variations. A 100 mW power from a fiber-pigtail module has been demonstrated. These lasers withstand severe thermal rollover tests without showing degradation effects. Preliminary lifetime tests indicate that their mean-time-to-failure (MTTF) may be very long, from several hundred thousand to one million hours, if not limited by sudden failure.
Risk Assessment on Suicide Death and Attempt among Chinese Rural Youths Aged 15–34 Years

Background: Although many suicide risk assessment tools are available in the world, their validity is not adequately assessed. In this study, we aimed to develop and evaluate a suicide risk assessment model among Chinese rural youths aged 15–34 years. Method: Subjects were 373 suicide deaths and 507 suicide attempters aged 15–34 years in three Chinese provinces (Shandong, Liaoning, and Hunan). Information about the community residents was also collected as the control groups. Social-demographic, social and psychological variables were examined for the suicides, suicide attempters, and community residents. Logistic regressions based on subjects from Shandong and Liaoning provinces were conducted to establish the suicide risk assessment models. Receiver operating characteristic (ROC) curves were drawn, and area under the ROC curves (AUC) were calculated to show how well the models separated the group being tested into those with and without suicide attempt or suicide. Results: The assessment model for suicide death included education years (OR = 0.773, p < 0.001), agricultural worker (OR = 2.091, p < 0.05), physical health (OR = 0.445, p < 0.05), family suicide history (OR = 6.858, p < 0.001), negative life events (OR = 1.340, p < 0.001), hopelessness (OR = 1.171, p < 0.001), impulsivity (OR = 1.151, p < 0.001), and mental disorder (OR = 8.384, p < 0.001). All these factors were also supported in the assessment model for suicide attempt, with an extension of very poor economic status (OR = 1.941, p < 0.01) and social interaction (OR = 0.855, p < 0.001). The AUC was 0.950 and 0.857 for the sample used to establish the assessment models of suicide death and attempt, respectively. The AUC was 0.967 and 0.942 for the sample used to verify the established assessment models of suicide death and attempt, respectively. Conclusions: Compared with some other assessment tools, the models for suicide death and attempt in the current study performed well among Chinese rural youths aged 15–34 years. A reliable suicide risk assessment approach, which includes multiple risk factors, should be evaluated in various cultures and populations.
β-lactam Resistance in Pseudomonas aeruginosa: Current Status, Future Prospects

Pseudomonas aeruginosa is a major opportunistic pathogen, causing a wide range of acute and chronic infections. β-lactam antibiotics including penicillins, carbapenems, monobactams, and cephalosporins play a key role in the treatment of P. aeruginosa infections. However, a significant number of isolates of these bacteria are resistant to β-lactams, complicating treatment of infections and leading to worse outcomes for patients. In this review, we summarize studies demonstrating the health and economic impacts associated with β-lactam-resistant P. aeruginosa. We then describe how β-lactams bind to and inhibit P. aeruginosa penicillin-binding proteins that are required for synthesis and remodelling of peptidoglycan. Resistance to β-lactams is multifactorial and can involve changes to a key target protein, penicillin-binding protein 3, that is essential for cell division; reduced uptake or increased efflux of β-lactams; degradation of β-lactam antibiotics by increased expression or altered substrate specificity of an AmpC β-lactamase, or by the acquisition of β-lactamases through horizontal gene transfer; and changes to biofilm formation and metabolism. The current understanding of these mechanisms is discussed. Lastly, important knowledge gaps are identified, and possible strategies for enhancing the effectiveness of β-lactam antibiotics in treating P. aeruginosa infections are considered.
Development of an Online Detection Setup for Dissolved Gas in Transformer Insulating Oil

The type and concentration of dissolved gases in transformer insulating oil are used to assess transformer conditions. In this paper, an online detection setup for measuring the concentration of multicomponent gases dissolved in transformer insulating oil is developed, which consists of an oil-gas separation system and an optical system for acquiring the transformer status in real time. The oil-gas separation system uses low pressure, constant temperature, and low-frequency stirring as working conditions for degassing large-volume oil samples based on modified headspace degassing. The optical system uses tunable diode laser absorption spectroscopy (TDLAS) to determine the gas concentration. Six target gases (methane, ethylene, ethane, acetylene, carbon monoxide, and carbon dioxide) were detected by three near-infrared lasers (1569, 1684, and 1532 nm). The stability of the optical system was improved by the common optical path formed by time-division multiplexing (TDM) technology. The calibration experiments show that the second harmonics and the concentrations of the six gases are linear. A comparison experiment with gas chromatography (GC) demonstrates that the error of acetylene reaches the nL/L level, while the other gases reach the μL/L level. The data conforms to the power industry testing standards, and the state of the transformer is analyzed by the detected six characteristic gases. The setup provides an effective basis for the online detection of dissolved gas in transformer insulating oil.
Current and Future Methodology for Quantitation and Site-Specific Mapping the Location of DNA Adducts

Formation of DNA adducts is a key event for a genotoxic mode of action, and their presence is often used as a surrogate for mutation and increased cancer risk. Interest in DNA adducts are twofold: first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. Methods have been established to quantitate DNA adducts with high chemical specificity and to visualize the location of DNA adducts, and elegant bio-analytical methods have been devised utilizing enzymes, various chemistries, and molecular biology methods. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. Herein, we briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technologies to achieve both measures. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.
Economic optimisation for an imperfect production system with rework and scrap rate

This paper developed an integrated model in considering the situations of an imperfect process with imperfect maintenance and shortage, and an imperfect rework process for the determination of economic production quantity. This imperfect process has a general deterioration distribution with increasing hazard rate. Even with periodic preventive maintenance, such a production system cannot be recovered as good as new. As the process produces non-conforming items, a percentage of them are scrapped and the others are reworked. There is a percentage of the reworking items fail the repairing and become scrap items in the imperfect rework process. We use numerical analyses to simulate the effect of changes in the various parameters on the optimal solution. And look at the optimal inspection interval, inspection frequency and production quantity that maximum total profit which the advantages of rework process were explored.
Convergence Variability and Population Sizing in Micro‐Genetic Algorithms

The issues of micro‐genetic algorithm (micro‐GA) convergence, population size, internal variability, and performance are examined. Procedures are developed to determine best population size when micro‐GAs are used for optimization of real‐world problems. Results show that the best population size cannot be determined before convergence has occurred and that convergence may occur without reaching an optimal solution. To determine the best population size, this article proposes the use of consistency of the final fitness values in addition to the fitness value at convergence. Internal variability must be considered in finding the best population size, and that best population size depends on the level of computational resources used. Smaller populations with generous computational resources performed as good as and sometimes better than larger populations. Midsized populations consistently exhibited lower variation in fitness value. For a given population size, the increase in fitness value may not be worth the added computation cost beyond a certain point. Three approaches are proposed to determine the best population size: (1) a random selection approach, (2) a detailed approach, and (3) a simplified approach. We used the detailed approach with our traffic problem and found that, with sufficient computational resources, the micro‐GA performed best with population size around the square root of the string length. We tested this proposition on standard simple and deceptive problems and found it to hold true.
Reliability Method of Cyber-Physical Systems Based on Events Polling and Interrupt Control

This paper proposed the reliability method of cyber-physical systems(CPS) based on events polling and interrupt control, discussed the reliability problem of CPS. According to the characteristic of polling principle and the CPS events model, proposed the polling principle of the CPS based on events. combined the method of the interrupt control, according to the spatio-temporal attributes of different events, set the different interrupt priority to solve the reliability problem of the CPS event model.
Improving Reproducibility of Crowdsourcing Experiments

This paper reports our preliminary investigation into which kinds of metadata are effective in improving the reproducibility of crowdsourcing experiments. We examined the effectiveness of using an ability distribution of workers, regarding their solving of tasks. One issue, however, is that identifying the ability required to solve a set of tasks can be difﬁcult. We applied item response theory (IRT) to ﬁnd said undeﬁned ability, allowing us to calculate the ability distribution of the workers. In our preliminary experiment, the standard deviation of the results quality was reduced to 60%. This implies that we can reduce the number repetitions of the experiment to 32.9%, while still maintaining the same power level.
Defect Analysis of 316 L Stainless Steel Prepared by LPBF Additive Manufacturing Processes

The 316 L stainless-steel samples were prepared by laser powder bed fusion (LPBF). The effects of processing parameters on the density and defects of 316 L stainless steel were studied through an orthogonal experiment. The density of the samples was measured by the Archimedes method, optical microscopy (OM) and X-ray Computed Tomography (XCT). The microstructures and defects under different LPBF parameters were studied by OM and SEM. The results show that the energy density has a significant effect on the defect and density of the structure. When the energy density is lower than 35.19 J/mm3, the density increases significantly with the increase of energy density. However, when the energy density is larger than this value, the density remains relatively stable. The process parameter with the greatest influence on energy density is the hatch distance D, followed by laser power P, scanning speed V and rotation angle θ. In this paper, the optimum parameters consist of P = 260 W, V = 1700 mm, D = 0.05 mm and θ = 67°, in which the density is as high as 98.5%. In addition, the possibility and accuracy of the XCT method in detecting the discontinuity and porosity of 316 L stainless steel were discussed. The results show that XCT can provide the whole size and variation trend of pores in the different producing direction of LPBF.
Diagnosis and Management of Suspected Pediatric Autoimmune Encephalitis: A Comprehensive, Multidisciplinary Approach and Review of Literature

Autoimmune encephalitis is an increasingly recognized entity in children. When treated promptly, favorable outcomes are seen in a majority of pediatric patients. However, recognition of autoimmune encephalitis in young patients is challenging. Once autoimmune encephalitis is suspected, additional difficulties exist regarding timing of treatment initiation and duration of treatment, as evidence to guide management of these patients is emerging. Here, we review available literature regarding pediatric autoimmune encephalitis and present our institution's comprehensive approach to the evaluation and management of the disease. These guidelines were developed through an iterative process involving both pediatric neurologists and rheumatologists.
Experiences with clinker grinding in roller mills

The recent experiences of applying roller mills to the production of finely ground products such as cement and slag are presented and reviewed. The unique process and operating conditions which need to be controlled and their impact on the success of the system are also discussed. The range of mill design features which are being used are shown and compared. The increase in the number and size of the finish grinding roller mills installed relates to their successful field experiences, reduced power requirements, increased confidence in reliability and simplicity of the installations. <<ETX>>
Evaluation of Crossability between Nicotiana benthamiana and Nicotiana excelsior

Wild tobacco species in the Nicotiana section Suaveolentes are promising genetic resources to introduce their disease resistance to cultivated tobacco, Nicotiana tabacum. However, hybrid lethality is observed in hybrid seedlings from crosses between most Suaveolentes species and N. tabacum. In particular, N. benthamiana belonging to the section Suaveolentes produces only viable hybrids after crossing with N. tabacum. In the present study, crossability between N. benthamiana and N. excelsior (section Suaveolentes) was investigated to test the possible usefulness of N. benthamiana as the bridge parent to transfer desirable genes of N. excelsior to N. tabacum via bridge crossing. After reciprocal crosses using three accessions of N. benthamiana and N. excelsior each, several crossing barriers such as cross-incompatibility, seed abortion, and male and female hybrid sterility were observed. Although reciprocal hybrids between N. benthamiana and N. excelsior showed a high degree of chromosome pairing in meiosis, univalents and multivalents, as well as chromosome bridges and lagging chromosomes, were observed. These meiotic abnormalities were thought to cause hybrid sterility. The possible usefulness of reciprocal hybrids between N. benthamiana and N. excelsior is discussed.
Nanoimprinted and Anodized Templates for Large-Scale and Low-Cost Nanopatterning

Nanopatterning to fabricate advanced nanostructured materials is a widely employed technology in a broad spectrum of applications going from spintronics and nanoelectronics to nanophotonics. This work reports on an easy route for nanopatterning making use of ordered porous templates with geometries ranging from straight lines to square, triangular or rhombohedral lattices, to be employed for the designed growth of sputtered materials with engineered properties. The procedure is based on large-scale nanoimprinting using patterned low-cost commercial disks, as 1-D grating stamps, followed by a single electrochemical process that allows one to obtain 1-D ordered porous anodic templates. Multiple imprinting steps at different angles enable more complex 2-D patterned templates. Subsequently, sputtering facilitates the growth of ferromagnetic antidot thin films (e.g., from 20 to 100 nm Co thick layers) with designed symmetries. This technique constitutes a non-expensive method for massive mold production and pattern generation avoiding standard lithographical techniques. In addition, it overcomes current challenges of the two-stage electrochemical porous anodic alumina templates: (i) allowing the patterning of large areas with high ordering and/or complex antidot geometries, and (ii) being less-time consuming.
Characterization of a Novel Thermostable Dye-Linked l-Lactate Dehydrogenase Complex and Its Application in Electrochemical Detection

Flavoenzyme dye-linked l-lactate dehydrogenase (Dye-LDH) is primarily involved in energy generation through electron transfer and exhibits potential utility in electrochemical devices. In this study, a gene encoding a Dye-LDH homolog was identified in a hyperthermophilic archaeon, Sulfurisphaera tokodaii. This gene was part of an operon that consisted of four genes that were tandemly arranged in the Sf. tokodaii genome in the following order: stk_16540, stk_16550 (dye-ldh homolog), stk_16560, and stk_16570. This gene cluster was expressed in an archaeal host, Sulfolobus acidocaldarius, and the produced enzyme was purified to homogeneity and characterized. The purified recombinant enzyme exhibited Dye-LDH activity and consisted of two different subunits (products of stk_16540 (α) and stk_16550 (β)), forming a heterohexameric structure (α3β3) with a molecular mass of approximately 253 kDa. Dye-LDH also exhibited excellent stability, retaining full activity upon incubation at 70 °C for 10 min and up to 80% activity after 30 min at 50 °C and pH 6.5–8.0. A quasi-direct electron transfer (DET)-type Dye-LDH was successfully developed by modification of the recombinant enzyme with an artificial redox mediator, phenazine ethosulfate, through amine groups on the enzyme’s surface. This study is the first report describing the development of a quasi-DET-type enzyme by using thermostable Dye-LDH.