Miracle-dz/UniFAR-FTUs huggingface dataset:
Emotion Recognition Using Spectral Feature from Facial Electromygraphy Signals for Human-Machine Interface

Recognition of the emotions demonstrated by human beings plays a crucial role in healthcare and human-machine interface. This paper reports an attempt to classify emotions using a spectral feature from facial electromyography (facial EMG) signals in the valence affective dimension. For this purpose, the facial EMG signals are obtained from the DEAP dataset. The signals are subjected to Short-Time Fourier Transform, and the peak frequency values are extracted from the signal in intervals of one second. Support vector machine (SVM) classifier is used for the classification of the features extracted. The extracted feature can classify the signals in the valence dimension with an accuracy of 61.37%. The proposed feature could be used as an added feature for emotion recognition, and this method of analysis could be extended to myoelectric control applications.
Draft Genome Sequences of Five Fungal Strains Isolated from Kefir

We present the annotated draft genome sequences of five fungal strains isolated from kefir grains. These isolates included three ascomycetous (Candida californica, Kazachstania exigua, and Kazachstania unispora) and one basidiomycetous (Rhodotorula mucilaginosa) species. The results revealed a detailed overview of the metabolic features of kefir fungi that will be potentially useful in biotechnological applications. ABSTRACT We present the annotated draft genome sequences of five fungal strains isolated from kefir grains. These isolates included three ascomycetous (Candida californica, Kazachstania exigua, and Kazachstania unispora) and one basidiomycetous (Rhodotorula mucilaginosa) species. The results revealed a detailed overview of the metabolic features of kefir fungi that will be potentially useful in biotechnological applications.
Genomic GC content drifts downward in most bacterial genomes

In every kingdom of life, GC->AT transitions occur more frequently than any other type of mutation due to the spontaneous deamination of cytidine. In eukaryotic genomes, this slow loss of GC base pairs is counteracted by biased gene conversion which increases genomic GC content as part of the recombination process. However, this type of biased gene conversion has not been observed in bacterial genomes, so we hypothesized that GC->AT transitions cause a reduction of genomic GC content in prokaryotic genomes on an evolutionary time scale. To test this hypothesis, we used a phylogenetic approach to analyze triplets of closely related genomes representing a wide range of the bacterial kingdom. The resulting data indicate that genomic GC content is drifting downward in bacterial genomes where GC base pairs comprise 40% or more of the total genome. In contrast, genomes containing less than 40% GC base pairs have fewer opportunities for GC->AT transitions to occur so genomic GC content is relatively stable or actually increasing. It should be noted that this observed change in genomic GC content is the net change in shared parts of the genome and does not apply to parts of the genome that have been lost or acquired since the genomes being compared shared common ancestor. However, a more detailed analysis of two Caulobacter genomes revealed that the acquisition of mobile elements by the two genomes actually reduced the total genomic GC content as well.
The Application of a Novel, Context Specific, Remote, Usability Assessment Tool to Conduct a Pre-Redesign and Post-Redesign Usability Comparison of a Telemedicine Website

The usability of telemedicine provider websites is an essential component of increasing the public's awareness of, understanding of, and safe and effective use of telemedicine. A newly developed usability data collection tool was used to conduct a usability assessment of the Teladoc website pre- and post- a redesign. The results suggest that the Teladoc website had better usability prior to the redesign. The Teladoc Website Usability Survey (TWUS) developed for the Teladoc website usability assessment can be modified and used in future usability studies of telemedicine provider interfaces. Usability research that takes place in specific context of use settings is more valuable to identify usability problems.
Nutrients and micronutrients at risk during renal replacement therapy: a scoping review

Supplemental Digital Content is available in the text Purpose of review Malnutrition is frequent in patients with acute kidney injury. Nutrient clearance during renal replacement therapy (RRT) potentially contributes to this complication. Although losses of amino acid, trace elements and vitamins have been described, there is no clear guidance regarding the role of micronutrient supplementation. Recent findings A scoping review was conducted with the aim to review the existing literature on micronutrients status during RRT: 35 publications including data on effluent losses and blood concentrations were considered relevant and analysed. For completeness, we also included data on amino acids. Among trace elements, negative balances have been shown for copper and selenium: low blood levels seem to indicate potential deficiency. Smaller size water soluble vitamins were found in the effluent, but not larger size liposoluble vitamins. Low blood values were frequently reported for thiamine, folate and vitamin C, as well as for carnitine. All amino acids were detectable in effluent fluid. Duration of RRT was associated with decreasing blood values. Summary Losses of several micronutrients and amino acids associated with low blood levels represent a real risk of deficiency for vitamins B1 and C, copper and selenium: they should be monitored in prolonged RRT. Further Research is urgently required as the data are insufficient to generate strong conclusions and prescription recommendations for clinical practice.
Contraceptive progestins with androgenic properties stimulate breast epithelial cell proliferation

Hormonal contraception exposes women to synthetic progesterone receptor (PR) agonists, progestins, and transiently increases breast cancer risk. How progesterone and progestins affect the breast epithelium is poorly understood because we lack adequate models to study this. We hypothesized that individual progestins differentially affect breast epithelial cell proliferation and hence breast cancer risk. Using mouse mammary tissue ex vivo, we show that testosterone‐related progestins induce the PR target and mediator of PR signaling‐induced cell proliferation receptor activator of NF‐κB ligand (Rankl), whereas progestins with anti‐androgenic properties in reporter assays do not. We develop intraductal xenografts of human breast epithelial cells from 36 women, show they remain hormone‐responsive and that progesterone and the androgenic progestins, desogestrel, gestodene, and levonorgestrel, promote proliferation but the anti‐androgenic, chlormadinone, and cyproterone acetate, do not. Prolonged exposure to androgenic progestins elicits hyperproliferation with cytologic changes. Androgen receptor inhibition interferes with PR agonist‐ and levonorgestrel‐induced RANKL expression and reduces levonorgestrel‐driven cell proliferation. Thus, different progestins have distinct biological activities in the breast epithelium to be considered for more informed choices in hormonal contraception.
TRPA1 and TRPV1, do we hold you in our heart?

Negative results contribute to the advancement of knowledge, at least as an argument of debate. A good example is given in this issue of Acta Physiologica by Hoebart et al. 1 who found no sign of life of the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel on cardiomyocytes despite a wide range of experimental approaches. Going fishing is always an exciting adventure, but coming home with the empty net is a bitter experience. Especially since you have to convince your entourage that you did your best, but that the sought-after fish was really absent from this lake, even if others have already reported traces of it. On the other hand, finding no traces of the "Loch Ness Monster", can, in some circumstances, provide a feeling of relief.
Mechanistic understanding and potential application of electrospraying in food processing: a review

Abstract Electrospraying (ESPR) is a cost effective, flexible, and facile method that has been used in the pharmaceutical industry, and thanks to its wide variety of uses such as bioactive compound encapsulation, micronization, and food product coating, which have received a great attention in the food market. It uses a jet of polymer solution for processing food and food-derived products. Droplet size can be extremely small up to nanometers and can be regulated by altering applied voltage and flow rate. Compared to conventional techniques, it is simple, cost effective, uses less solvent and products are obtained in one step with a very high encapsulation efficiency (EE). Encapsulation provided using it protects bioactives from moisture, thermal, oxidative, and mechanical stresses, and thus provides them a good storage stability which will help in increasing the application of these ingredients in food formulation. This technique has an enormous potential for increasing the shelf life of fruit and vegetables through coating and improvement of eating quality. This study is aimed at overviewing the operating principles of ESPR, working parameters, applications, and advantages in the food sector. The article also covers new ESPR techniques like supercritical assisted ESPR and ESPR assisted by pressurized gas (EAPG) which have high yield as compared to conventional ESPR. This article is enriched with good information for research and development in ESPR techniques for development of novel foods.
The impact of temporary contracts on suicide rates

The number of suicides has increased in the last decades in several developed countries. For instance, Spain has experienced a gradual but steady increase in suicides since the 80’s and it is currently the leading external cause of death in the country. At the same time, the dualisation of the labor market, with a strong and persistent incidence of temporary contracts, has increased the instability of employment conditions. Both developments have a stronger incidence for individuals with lower levels of education. Therefore, in this paper we use rich administrative data in order to estimate the impact of the wide spread use of temporary contracts on suicides. In order to do that we exploit a reform that liberalised the use of fix-term contracts in Spain in 1984. Our results show strong long-term effects of the reform, which increased the suicide mortality rate of affected cohorts (those entering the labor market just after the liberalisation) by at least 25.3%. We believe that this result has important policy implications and should be taken into account in the design of the national suicide prevention plans.
Anticentromere Antibody Levels and Isotypes and the Development of Systemic Sclerosis

Little is known on the disease course of very early systemic sclerosis (SSc). Among the information yet to be elucidated is whether anticentromere antibody (ACA) isotype levels can serve as biomarkers for future SSc development and for organ involvement. This study was undertaken to evaluate whether IgG, IgM, and IgA ACA levels in IgG ACA–positive patients are associated with disease severity and/or progression from very early SSc to definite SSc.
Merging Genomics Public Datasets with Clinical Cancer Registry Data - Lessons Learned

Publicly available datasets - for example via cBioPortal for Cancer Genomics - could be a valuable source for benchmarks and comparisons with local patient records. However, such an approach is only valid if patient cohorts are comparable to each other and if the documentation is complete and sufficient. In this paper, records from exocrine pancreatic cancer patients documented in a local cancer registry are compared with two public datasets to calculate overall survival. Several data preprocessing steps were necessary to ensure comparability of the different datasets and a common database schema was created. Our assumption that the public datasets could be used to augment the data of the local cancer registry could not be validated, since the analysis on overall survival showed a significant difference. We discuss several reasons and explanations for this finding. So far, comparing different datasets with each other and drawing medical conclusions on such comparisons should be conducted with great caution.
Genome Sequencing Identified a SARS-CoV-2 Lineage B.1.1.7 Strain with a High Number of Mutations from Dhaka, Bangladesh

We report a coding-complete genome sequence of the SARS-CoV-2 strain SARS-CoV-2/BGD/GC001, isolated from a Bangladeshi patient with respiratory symptoms. Phylogenetic analysis assigned this strain to lineage B.1. 1.7, which presented a total of 36 mutations in the spike and other genomic regions compared to strain Wuhan Hu-1 (GenBank accession number NC_045512.2). ABSTRACT We report a coding-complete genome sequence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain SARS-CoV-2/BGD/GC001, isolated from a Bangladeshi patient with respiratory symptoms. Phylogenetic analysis assigned this strain to lineage B.1. 1.7, which presented a total of 36 mutations in the spike and other genomic regions compared to strain Wuhan Hu-1 (GenBank accession number NC_045512.2).
Repurposing Case-Based Learning to a Conversational Agent for Healthcare Cybersecurity

A crucial factor for successful cybersecurity education is how information is communicated to learners. Case-based learning of common cybersecurity issues has been shown to improve human behaviour for prevention. However, some delivery methods prevent realistic critical appraisal and reflection of awareness. Conversational agents can scaffold healthcare workers' understanding and promote deterrence strategies. The challenges of repurposing material to create a case-based agent were explored, and the ASPIRE process was modified. Heuristic evaluation from 10 experts in innovative educational technology resulted in the desired outcomes of usability, however Natural Language Understanding improvements were needed. Discussion of best practice when repurposing into conversational agents suggested modification of the ASPIRE process is feasible for future use.
Comparing SDTM and FHIR® for Real World Data from Electronic Health Records for Clinical Trial Submissions

Real world data for use in clinical trials is promising. We compared the SDTM for clinical trial data submission with FHIR® for routine documentation. After categorization of variables by relevance, clinically relevant SDTM items were mapped to FHIR®. About 30% in both were seen as clinically relevant. The majority of these SDTM items were mappable to FHIR® Observation resource.
Growth Kinetics and Electrical Properties of Ultrathin Silicon Dioxide Layers

In this paper, the growth kinetics and electrical properties of ultrathin silicon-dioxide layers are reviewed. Topics discussed here include the onset of oxide growth, the effects of temperature, substrate orientation, dopant type and concentration, and stress on oxide growth kinetics. The use of in situ real-time ellipsometry to control the rapid-thermal oxidation of silicon will be presented. The electrical properties of the ultrathin silicon-dioxide layers will be discussed, especially the contact potential difference in MOS devices and its dependence on the processing history and substrate orientation which will be explained in terms of the role of partial-charge-transfer dipoles at the oxide/silicon interface. Finally, gate tunneling considerations in MOSFET design, modeling, characterization, and circuit performance will be briefly presented.
Blood–Brain Barrier Breakdown in Relationship to Alzheimer and Vascular Disease

Blood–brain barrier (BBB) breakdown has been suggested to be an early biomarker in human cognitive impairment. However, the relationship between BBB breakdown and brain pathology, most commonly Alzheimer disease (AD) and vascular disease, is still poorly understood. The present study measured human BBB function in mild cognitive impairment (MCI) patients on 2 molecular scales, specifically BBB's permeability to water and albumin molecules.
Digital Values for Alpha Acuities.

PURPOSE The lack of consistent data formatting for visual acuity in electronic medical records has led to inefficient data extraction and analysis. METHODS Prior acuity efforts were reviewed and analyzed relative to alpha-designated visual acuity. Prominent electronic medical record formatting was reviewed. RESULTS A table was generated facilitating the assignment of digital values to alpha acuities for metric and United States designation including centered steady maintained for infants and counting fingers, hand motions, light perception, and no light perception for patients with low vision. DISCUSSION Consistent and usable visual acuity data should lead to better research and eventually improve physician burn-out. [J Pediatr Ophthalmol Strabismus. 2021;58(2):132-135. ].
Draft Genome Sequences of 40 Dermatophilus congolensis Isolates from Bovine Dermatophilosis Cases in St. Kitts and Nevis

Dermatophilus congolensis causes dermatophilosis in cattle, mainly in tropical climates. Despite the economic losses caused by this bacterium, its pathogenic factors are less well understood. We report draft genomes of D. congolensis strains isolated during a dermatophilosis outbreak in cattle in St. Kitts and Nevis. Some isolates contain tet(Z), which is responsible for resistance to tetracyclines. ABSTRACT Dermatophilus congolensis causes dermatophilosis in cattle, mainly in tropical climates. Despite the economic losses caused by this bacterium, its pathogenic factors are less well understood. We report draft genomes of D. congolensis strains isolated during a dermatophilosis outbreak in cattle in St. Kitts and Nevis. Some isolates contain tet(Z), which is responsible for resistance to tetracyclines.
The long‐term incidence of dysplasia and colorectal cancer in a Crohn’s colitis population‐based cohort

The risk of developing colorectal cancer (CRC) in Crohn's disease (CD) has been variably reported. Chronic inflammation is associated with an increased risk of neoplasia; variable outcomes in CD possibly reflect the heterogeneous nature of the disease. The aim of this work was to characterize the risk of CRC in a New Zealand population‐based cohort of CD patients with colonic inflammation.
The effect of species, size, and fire intensity on tree mortality within a catastrophic bushfire complex.

Infrequent, high-intensity disturbances can have profound impacts on forested landscapes, changing forest structure and altering relative species abundance. However, due to their rarity and the logistical challenges of directly observing such extreme events, both the spatial variability of disturbance intensity and the species-specific responses to this variability are poorly understood. We used observed patterns of mortality across a fire severity gradient following the 2009 Black Saturday fires in southeastern Australia to simultaneously estimate 1) species- and size-specific susceptibility to fire-induced mortality and 2) fire intensity. We found broad variation in patterns of fire susceptibility among the 10 tree species (five eucalypts and five non-eucalypts) sufficiently abundant for analysis. Among the eucalypts, Eucalyptus obliqua was the most resistant to fire-induced mortality, with trees of ~25 cm DBH having a 50% probability of surviving even the most intense fires. In contrast, E. regnans had 100% mortality across all size classes when subjected to high intensity fire. Basal resprouting occurred in six of the study species and, when accounted for, fundamentally changed the mortality profile of these species, highlighting the importance of resprouting as an adaptation to fire in these landscapes. In particular, the two iconic cool temperate rainforest species (Nothofagus cunninghami and Atherosperma moschatum) were strong resprouters (~45% of individuals were able to resprout after being top-killed by fire). We also found evidence for compositional shifts in regeneration above threshold values of fire intensity in cool temperate rainforest and mixed forest sites, both of which have important conservation values within these landscapes. The observed patterns of species- and size-specific susceptibility to fire-induced mortality may be used to anticipate changes in forest structure and composition in the future. In addition, they may also help guide forest management strategies that reduce the length of time individual trees are exposed to potentially lethal fires, thereby increasing the resilience of these forests to future fires.
Medical Students Learning on the COVID-19 Front Line

In this viewpoint, we share our perspectives, as medical students at Imperial College London, on our experiences during our Infectious Diseases placement at Northwick Park Hospital, touching upon other students’ experiences at other sites as well. We highlight some of the main drivers of and barriers to medical students seeing patients with COVID-19.
Transient receptor potential polycystin‐2 (TRPP2) regulates motility and intracellular calcium of porcine sperm

Polycystin‐2, also known as transient receptor potential polycystin‐2 (TRPP2), is a membrane protein that regulates calcium homeostasis in renal epithelial cells. Mutations in PKD2, the gene encoding human TRPP2, cause enlarged cystic kidneys and contribute to polycystic kidney disease (PKD). Male Drosophila melanogaster with mutations in amo, the homolog of PKD2, display a mild decrease in sperm motility but have a drastic reduction in fertility due to failed sperm migration and storage within the female tract. Although TRPP2 has critical roles for Drosophila sperm function, the protein has not been described in mammalian sperm. Herein, we report the localization of TRPP2 in porcine sperm and identify functions of TRPP2 in regulating intracellular Ca2+ and motility. Porcine sperm treated with an antibody to TRPP2 in capacitating medium had reduced average path velocity and curvilinear velocity (p < .05). Blocking TRPP2 also increased sperm tail beat‐cross frequency (p < .05). After 90 min of capacitation, sperm incubated with TRPP2 antibody had decreased intracellular Ca2+ concentration compared to controls (p < .05), consistent with TRPP2 function as a plasma membrane cation channel. This is the first report that mammalian sperm contain TRPP2, which appears to regulate intracellular Ca2+ and motility patterns in porcine sperm.
Use of contrast enhanced ultrasound in testicular diseases: A comprehensive review

Contrast‐enhanced ultrasound (CEUS) is a sonographic technique that increases the diagnostic accuracy of ultrasound and color Doppler ultrasound (CDUS) when studying testicular abnormalities. However, its role in clinical practice is still debatable because there are no accepted standards regarding how and when this technique should be used for patients with testicular disease.