Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell migration inducing hyaluronidase 1 promotes growth and metastasis of papillary thyroid carcinoma

ABSTRACT Cell migration inducing hyaluronidase 1 (CEMIP) mediates catabolism of hyaluronan, and participates in the cell metastasis, invasion, and motility. Dysregulated CEMIP expression was associated with progression and prognosis of tumors. The role of CEMIP in papillary thyroid carcinoma (PTC) remains unknown. Our study showed that CEMIP was upregulated in both tissues and cells of PTC. Silencing of CEMIP reduced cell proliferation and suppressed migration and invasion of PTC. Protein expression of phosphorylated STAT3 (Signal Transducer And Activator Of Transcription 3) (p-STAT3), AKT (p-AKT) and p65 (p-p65) were decreased by CEMIP silencing in PTC cells. Pyruvate dehydrogenase kinase 4 (PDK4) over-expression attenuated CEMIP silencing-induced decrease in p-STAT3, p-AKT and p-p65. Silencing of CEMIP-induced decrease in cell proliferation and metastasis in PTC were restored by over-expression of STAT3. CEMIP functioned as an oncogenic gene in PTC through PDK4-mediated activation of STAT3/AKT/NF-κB pathway. Graphical abstract
A Comparison of the Timing of Hand Expressing of Human Milk With Breast Massage to Standard Care for Mothers of Preterm Infants: An Exploratory Pilot Using a Randomized Controlled Design

Background: Globally, 10% of all births are preterm. Access to human milk via manual breast expression is required to reduce the incidence of adverse outcomes related to prematurity. However, there is little evidence to recommend optimum timing to commence breast expression in mothers of preterm infants or the most effective method. Research Aims: (1) To test feasibility of recruitment and compliance to the protocol and (2) to determine influence of using hand expressing and breast massage on milk production, engorgement, mastitis, and breastfeeding status at 3 months. Methods: This study was an exploratory parallel two-group, pilot randomized controlled trial. Mothers of preterm infants at a metropolitan maternity hospital in Queensland Australia (N = 31) were randomized to receive either hand expressing and breast massage within the 1st hr of birth or standard care, hand expressing within 6 hr of birth, to determine the influence on milk production, engorgement, mastitis, and breastfeeding status at 3 months. Results: Feasibility targets were not met; however, valuable learning from this trial uncovered barriers facing midwives in the birth suite to commencing expressing in the 1st hr of birth. There was no difference in occurrence of secondary outcomes, although trends support future study. Conclusions: Overall, unpredictability of preterm birth influenced our ability to recruit participants. Important directions for future study design would benefit from incorporating expressing times up to 6 hr with a replicable breast massage.
In silico models to predict tubular secretion or reabsorption clearance pathway using physicochemical properties and structural characteristics

Abstract Renal clearance is one of the main pathways for a drug to be cleared from plasma. The aim of this study is to develop in-silico models to find out the relationship between the type of renal clearance, and structural parameters. Literature data were used to categorise the drugs into those that undergo tubular secretion and those that undergo reabsorption. Different structural descriptors (VolSurf descriptors, Abraham solvation parameters, data warrior descriptors, logarithm of distribution coefficient at pH = 7.4 (logD7.4)) were applied to develop a mechanistic model for estimating renal clearance class whether its secretion or reabsorption. The results of this study show that logD7.4 and the number of hydrogen bond donors, as well as available uncharged species (AUS7.4), are the most effective descriptors to establish mechanistic models for predicting renal clearance class. The classification models were established with a level of accuracy of more than 75%. Developed models of this study can be helpful to predict renal clearance class for new drug candidates with an acceptable error. Hydrophilicity and hydrogen bond formation ability of drugs are among the main descriptors.
Multi‐information source Bayesian optimization of culture media for cellular agriculture

Culture media used in industrial bioprocessing and the emerging field of cellular agriculture is difficult to optimize due to the lack of rigorous mathematical models of cell growth and culture conditions, as well as the complexity of the design space. Rapid growth assays are inaccurate yet convenient, while robust measures of cell number can be time‐consuming to the point of limiting experimentation. In this study, we optimized a cell culture media with 14 components using a multi‐information source Bayesian optimization algorithm that locates optimal media conditions based on an iterative refinement of an uncertainty‐weighted desirability function. As a model system, we utilized murine C2C12 cells, using AlamarBlue, LIVE stain, and trypan blue exclusion cell counting assays to determine cell number. Using this experimental optimization algorithm, we were able to design media with 181% more cells than a common commercial variant with a similar economic cost, while doing so in 38% fewer experiments than an efficient design‐of‐experiments method. The optimal medium generalized well to long‐term growth up to four passages of C2C12 cells, indicating the multi‐information source assay improved measurement robustness relative to rapid growth assays alone.
Prediction of binding affinity of 1,2-diphenyline ketone analogues at adenosine triphosphate binding site of glycogen synthase kinase-3β: a molecular docking and dynamic simulation study

Abstract Glycogen synthase kinase (GSK)-3β is one of the downstream signalling molecules involved in phosphorylation of glycogen synthase, a key enzyme involved in the synthesis of glycogen from glucose. GSK-3β regulate some of the critical processes underlying structural and functional synaptic plasticity of neurons. Down regulation or inhibition of GSK-3β enhances long-term potentiation and cognitive functions in animal models of Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. A number of compounds are available to inhibit GSK-3β, however none of them are in clinical practice to treat neurodegenerative diseases. The aim of our study was to predict the molecular interaction and dynamic behaviour of naturally occurring 1,2-diphenyline ketone analogues at the adenosine triphosphate binding site of glycogen synthase kinase (GSK)-3β through simulation studies. Out of all 1,2-diphenyline ketone analogues,1, 3, 5, 6-Tetrahydroxyxanthone (Rank = 1), Secalonic acid F (Rank = 2), and Trihydroxy-2-(2,3-dihydroxy-3-methylbutyl)-7-methoxy-8-(3-methyl-2-butenyl) xanthone (Rank = 3) were found to exhibit lowest docking score of −12.07, −11.49, and −11.24 kcal/mol with dissociation constant of 1.37, 3.84, and 5.99 nM, respectively. The molecular dynamic simulation of rank 1 and rank 3 ligands indicated stable interaction throughout the simulation and interaction analyses has shown that the presence of hydroxyl groups at C1, C3, C5, and C6 around 1,2 diphenyline ketone nucleus to influence their binding affinity at the ATP-binding site of GSK-3β. We predicted that 1,3,5,6-Tetrahydroxyxanthone and 1, 3, 6-Trihydroxy-2-(2,3-dihydroxy-3-methylbutyl)-7-methoxy-8-(3-methyl-2-butenyl) xanthone may act as a potential ligand or lead compound to inhibit GSK-3β and also may play an important role in alleviating neurodegenerative diseases. Communicated by Ramaswamy H. Sarma
CASCA E COCO DE MACAÚBA ADICIONADOS AO CONCENTRADO PARA VACAS MESTIÇAS LACTANTES EM DIETAS À BASE DE SILAGEM DE MILHO

The possibility of use of waste generated in the production of biodiesel can be valuable in feeding ruminants, as the quantity of waste is great; Thus, the final disposal must be appropriately. It was tested the feasibility of using coco and coco bark residue replacing corn and soybean meal in the concentrate in 0, 0.4; 0.8 and 1.2 kg in a total of 2.5 kg/cow/day. The concentrate was given to dairy cows at milking time, twice a day, and the silage in the morning. Eight Hosltein-Gyr crossbred cows were used, being distributed in two 4 x 4 Latin squares, with an average of 11 kg of milk/day. The chemical analyses of milk were made in Embrapa Gado de Leite in Juiz de Fora-MG. Milk production and body weight were measured at the end of experimental period of 10 days. Analysis of variables was made in Latin square design. The statistical model included effects of treatment (replacement levels), Latin square (QL), animal/QL and period/QL at 5% probability. There was no difference in milk production. The levels of lactose, dry extract and degreased dried extract were significantly different, however the daily production in kg/day were not different. The bark and coconut of Macaw Palm can be used until 1.2 kg, out of a total of 2.5 kg of the concentrate / cow / day without affecting the performance of crossbred cows producing approximately 11 kg of milk per day in corn silage based diet.
Effects of fire on CO2 , CH4 , and N2O exchange in a well‐drained Arctic heath ecosystem

Wildfire frequency and expanse in the Arctic have increased in recent years and are projected to increase further with changes in climatic conditions due to warmer and drier summers. Yet, there is a lack of knowledge about the impacts such events may have on the net greenhouse gas (GHG) balances in Arctic ecosystems. We investigated in situ effects of an experimental fire in 2017 on carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) surface fluxes in the most abundant tundra ecosystem in West Greenland in ambient and warmer conditions. Measurements from the growing seasons 2017 to 2019 showed that burnt areas became significant net CO2 sources for the entire study period, driven by increased ecosystem respiration (ER) immediately after the fire and decreased gross ecosystem production (GEP). Warming by open‐top chambers significantly increased both ER and GEP in control, but not in burnt plots. In contrast to CO2, measurements suggest that the overall sink capacity of atmospheric CH4, as well as net N2O emissions, were not affected by fire in the short term, but only immediately after the fire. The minor effects on CH4 and N2O, which was surprising given the significantly higher nitrate availability observed in burnt plots. However, the minor effects are aligned with the lack of significant effects of fire on soil moisture and soil temperature. Net uptake and emissions of all three GHG from burnt soils were less temperature‐sensitive than in the undisturbed control plots. Overall, this study highlights that wildfires in a typical tundra ecosystem in Greenland may not lead to markedly increased net GHG emissions other than CO2. Additional investigations are needed to assess the consequences of more severe fires.
ARCHITECTURAL, ARCHAEOLOGIC AND ENVIRONMENTAL RESTORATION PLANNING METHODOLOGY: HISTORIC RESEARCHES AND TECHNIQUES OF SURVEY AIMING TO CONSERVATION

In the modern culture of conservation, the historic authenticity of cultural property arises as a prioritary value, asserting that restoration is never based on aleatory hypothesis. Being understood that for each monument and site to restore we have to realize a specific and singular study from which a peculiar planning solution can spring, it is moreover possible to identify a research process for the restoration based on three essential moments: a) the “connotation”, allowing us to know the object with the necessary critical comprehension; b) the “diagnosis”, where we effect the reading and the recognizing of the state of decay and we identify all the available means to guarantee the physical conservation of the work; c) the definition of the “ plan”, through which the utilization of the monument is realized, for the aims proposed and within the bounds allowed by the integrated conservation. Therefore, before operating any intervention, the restorer must reach the deep and complete knowledge of the cultural property, through a deepened bibliographic and archival research, the gathering of the detailed iconographic documentation even through prints, drawings, ancient photographs, with the double aim to documentate, on one side, the existing situation before the restoration, and on the other side, to control the exactness of the survey we are realizing, in order to supply with documentary evidence, not only the history of construction, but also all the existing deformations and anomalies, the analysis of the decay, the evaluation of damages, the analysis of the fissuring panel, the knowledge of which is necessary to fully understand the stability conditions. From here the exigency of achieving the physical knowledge of the restoration objects, through a high quality level survey, not leading to the representation of a generic, approximate or conjectural shape, but giving instead the «effective shape» that is to say «perfectly objective, total, with all its irregularities, wanted or not, relevant or not, having an aesthetic, technical or historical interest» (Hans
A comprehensive evaluation of factors affecting nurse leaders’ work-related well-being

Purpose This study aims to describe nurse leaders’ experiences of work-related well-being and its association with background variables, working conditions, work engagement, sense of coherence and burnout. Design/methodology/approach An electronic survey design was used. Data was collected between December 2015 and May 2016 with an instrument that included demographic questions and four internationally validated scales: the Utrecht Work Engagement Scale, QPS Nordic 34+, the shortened Sense of Coherence scale and the Maslach Burnout Inventory. Data was analysed using statistical methods. Findings A total of 155 nurse leaders completed the questionnaire, giving a 44% response rate. Most of them worked as nurse managers (89%). Participants’ work-related well-being scores ranged from 8 to 10. Statistically significant relationships were found between participants’ work-related well-being and their leadership skills, current position, sense of coherence and levels of burnout. In addition, there were statistically significant relationships between work-related well-being and all dimensions of working conditions. Originality/value This study underlines the fact that work-related well-being should not be evaluated based on a single factor. The participants’ perceived work-related well-being was high, although almost half of them reported always or often experiencing stress. The results suggest that nurse leaders may have resources such as good leadership and problem-solving skills, supportive working conditions and a high sense of coherence that prevent the experienced stress from adversely affecting their work-related well-being.
Exploring job demands and resources in psychotherapists treating displaced people—A scoping review

Abstract Objective: Little is known about the occupational health of psychotherapists treating displaced people. Therefore, the literature was explored regarding job demands, job resources, as well as work- and health-related outcomes. The Job Demands-Job Resources model (JD-R model) according to Bakker and Demerouti served as theoretical framework. Method: This scoping review followed the methodological framework provided by the Joanna Briggs Institute (JBI). Six databases were searched for qualitative, quantitative and mixed methods studies. Results: Based on 15 included articles, the emotional impact of work, cultural or language barriers, and administrative obstacles were identified as major job demands. Important job resources were related to a politicized conceptualization of the professional identity. Health-related outcomes could mainly be ascribed to empathy-based strain, while work-related outcomes were associated with discrepancies between personal values and asylum regulations. Conclusion: Despite the emotional impact of work, psychotherapists seem to benefit from work engagement arising out of socio-political conviction and the ability to help suffering people. Practitioners need more environmental support like professional networks. Further research is required, as current findings are predominantly based on qualitative and cross-sectional studies.
Spatial analysis of acoustic noise transfer function with a human-body phantom in a clinical MRI scanner

Background The acoustic noise in magnetic resonance imaging (MRI) potentially depends on the measurement position and presence of a patient inside the scanner bore. Purpose To analyze the spatial characteristics of the acoustic noise by using the gradient-pulse-to-acoustic-noise transfer function (GPAN-TF) with and without a human-body phantom on the examination table. Material and Methods Acoustic noise waveforms were acquired at 80 and 110 measurement positions with and without a phantom. The GPAN-TFs µPa/(mT/m) in the coils were calculated by deconvolution. The phantom effect on the spatial distribution of the acoustic noise was assessed using the peak sound pressure levels (SPLs), mean values, peak values, and peak frequencies of the GPAN-TFs. Results The peak SPLs in all positions for the X-, Y-, and Z-gradient coils were increased by 11.1 dB, 1.4 dB, and 6.1 dB, respectively, compared with the peak SPL of the magnetic isocenter. The maximum peak SPLs among all positions of the X-, Y-, and Z-gradient coils with the phantom were increased by 4.9 dB, 7.4 dB, and 6.9 dB, respectively, relative to those without the phantom. However, the peak SPLs decreased at some positions with the phantom placed on the table (X-gradient coil = 4.6 dB, Y-gradient coil = 5.0 dB, Z-gradient coil = 8.4 dB). The most common peak frequencies were in the range of 2000–3000 Hz. Conclusion “Hotspot” areas with and without the phantom were associated with acoustic noise sources in the clinical MRI scanner and were enhanced by the phantom's presence.
Ethosomal gel for rectal transmucosal delivery of domperidone: design of experiment, in vitro, and in vivo evaluation

Abstract Despite high efficiency of domperidone (DOM) in prophylaxis of emesis accompanied with radiotherapy and chemotherapy, it still can bother cancer patients by its powerful side effects and difficulty of its oral administration. The study was designed to develop and optimize DOM loaded ethosomal gel for rectal transmucosal delivery. Ethosomal formulations were prepared using a 21, 51 full-factorial design where the impact of lecithin concentration and additives were investigated. The optimum ethosomal vesicles were subsequently incorporated in Carbopol gel base where rheological behavior, spreadability, mucoadhesion, and in vivo pharmacokinetic parameters were studied. Based on Design Expert® software (Stat Ease, Inc., Minneapolis, MN), the optimum formulation illustrated entrapment efficiency of 70.02%±5.52%, and vesicular size of 112 ± 3.3 nm, polydispersity index of 0.32 ± 0.01, zeta potential of −59 ± 0.28 mV, and % drug released after 6 h of 76.30%±2.45%. Moreover, ex vivo permeation through rabbit intestinal mucosa increased four times compared to free DOM suspension. The gel loaded with ethosomes showed excellent mucoadhesion to rectal mucosa. DOM ethosomal gel showed a raise in C max and AUC0–48 of DOM by twofolds compared to free DOM gel. The study suggested that ethosomes incorporated in gels could be an efficient candidate for rectal transmucosal delivery of DOM.
Exploration of rehabilitation through the use of virtual reality interventions for patients with hand conditions: a scoping review

The use of virtual reality to aid the rehabilitation of hand and upper-limb conditions has been an emerging field over the past decade. The majority of research in this field has been focused on neurological conditions for example post-stroke, with major advances being made to improve hand function through various robotic and digital techniques. In spite of the technological advancements in virtual reality rehabilitation, it is not readily used in current practice for hand or upper-limb conditions. The recent crisis of COVID-19, and the requirements and/or preferences of some people to receive rehabilitation at home have highlighted the importance of developing this technology so that it could become part of standard practice. This scoping review was conducted to explore research addressing immersive virtual reality in hand rehabilitation. The objectives were to identify the strengths and weaknesses of current technology and highlight any gaps in knowledge which could inform future research.
A Retrospective Analysis of Clinical Characteristics, Treatment Modalities and Outcome of the Patients With Infantile Hepatic Hemangiomas: Single-center Experience From Turkey

Objective: Hepatic hemangiomas (HH) are the most common vascular tumors of the liver. It is important to distinguish hemangiomas from malignant liver tumors. Materials and Methods: The patients 0 to 1 years old, were diagnosed with HH and followed up in the oncology outpatient clinic between 2009 and 2020 were included in the study. Results: A total of 127 patients with the diagnosis of HH were included in the study. Of the patients, 99 (78%) had focal, 20 (15.7%) had multifocal, and 8 (6.3%) had diffuse HHs. Surgery was performed and the diagnosis was confirmed histopathologically in 6 patients (4.7%). During the follow-up, 16 (12.5%) patients received medical treatment. Thirteen (10.2%) were treated with propranolol, 2 (1.5%) with corticosteroids, and 1 (0.8%) with propranolol and corticosteroids. Complete response was obtained in 9 (9/16) patients and partial response was obtained in 6 (6/16) patients with medical treatment. Conclusion: Although HH is a benign tumor, it is important to make its differential diagnosis with malignant tumors of the liver. Over the years, the need for histopathologic examination for diagnosis has decreased. The success rate of propranolol is high, and the need for other treatment options with a high side-effect profile has decreased significantly since 2008.
Development of Servo-Units for a Compliantly Actuated Quadruped Prototype

This master thesis deals with the development of servo-motor-units as part of the quadruped project at the Institute of Robotics and Mechatronics, German Aerospace Center (DLR). To be able to perform highly dynamic motions such as jumping, the servo-motor-units of a quadruped robot require to produce high torque and velocity peaks on the one hand while being of compact size and light weight on the other hand. This is achieved by means of taking an off the shelf servo-motor-unit and replacing the electronics by a custom electronics which requests the maximum power of the motors. The developed servo-motor-unit provides a compact powerful motor with a good position sensor and a real-time USB link. The firmware enables to command the motor with a frequency of 1 kHz. The frequency of control loop and the communication can be set independently from each other. In a second part the development of an automated test system for motors is described. The measurements showed that performance factors and the efficiency of the developed servo-motor-unit are almost on the same level of a high-end motor, specially designed for application in robotic systems. It is interesting that the servo-motor-unit is almost as efficient as a high-end robot motor, but is about factor 10 cheaper.