Miracle-dz/UniFAR-FTUs huggingface dataset:
Corrosion Protection Oxide Scale Formed on Surface of Fe-Ni-M (M = Al, Cr, Cu) Inert Anode for Molten Salt Electrolysis

An oxide scale formed on the surface of metal anodes is crucial for determining the overall quality of molten salt electrolysis (MSE), particularly for the durability of the anode materials. However, the material properties of oxide scales are yet to be revealed, particularly in ternary spinel oxide phases. Therefore, we investigate the mechanical and thermal properties of spinel oxides via first-principles calculations. The oxides are calculated using the models of normal (cubic) and inverse (orthorhombic) spinel compounds. The d-orbital exchange correlation potential of transition metal oxides is addressed using the generalized gradient approximation plus Hubbard U. The lattice constant, formation energy, cohesive energy, elastic modulus, Poisson’s ratio, universal anisotropy index, hardness, minimal thermal conductivity, and thermal expansion coefficient are calculated. Based on the calculated mechanical and thermal properties of the spinel compound, the Fe–Ni–Al inert anode is expected to be the most suitable oxide scale for MSE applications among the materials investigated in our study.
Silencing CDCA8 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by blocking the Akt pathway

Purpose: To investigate the effect of cell division cycle associated 8 (CDCA8) on malignant progression of gastric cancer (GC) cells. Methods: Short hairpin RNAs (shRNA) were transfected into two gastric cell lines to knock down expression of CDCA8. Transfection efficiency was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Then, Cell Counting Kit 8 and colony formation and Transwell assays were utilized to explore the effect of CDCA8 knockdown on the proliferation, invasion, and migration of GC cells. Flow cytometry was conducted to analyze the effect of CDCA8 knockdown on cell cycle progression and apoptosis. The relationship between CDCA8, epithelial-mesenchymal transition (EMT), and Akt pathway activity was determined by western blot analysis. Results: Proliferation, invasion, and migration of GC cells were significantly inhibited by CDCA8 knockdown. Knockdown of CDCA8 induced cell cycle arrest in G1 phase and apoptosis, and inhibited EMT and Akt pathway activity. Conclusion: Knockdown of CDCA8 inhibits GC growth and metastasis in vitro by reducing Akt pathway activity. Thus, this molecule presents a potential strategy for the management of GC.
Comparison of Recruitment Patterns of Sessile Marine Invertebrates According to Substrate Characteristics

A community of benthic invertebrates, including sessile adult-stage invertebrates, can negatively effect corrosion, deformation, and increased fuel consumption by attaching to artificial structures, a phenomenon known as marine biofouling. Investigating the relationship between benthic communities and artificial structures or substrates (to which the organisms attach) can help clarify the factors influencing marine biofouling. Therefore, in our study, natural (stone) and artificial (rubber, tarpaulin, and iron) substrates were installed in three harbors (Mokpo, Tongyeong, and Busan), and the structures of the communities attached to each substrate were compared. The total study period was 15 months (September 2016 to December 2017), and field surveys were performed at 3-month intervals. The three survey sites had significant differences in the structure of the sessile community present. In particular, Tongyeong was significantly different from Mokpo and Busan due to the continuous dominance of Cirripedia. When comparing natural and artificial substrate by sites, significant differences were observed in the community structure in all three surveyed sites. In Mokpo and Busan, colonial ascidians were dominant on natural substrate rather than artificial substrates; post-summer, Cirripedia coverage was higher on artificial substrates than natural substrate due to corrosion. Tongyeong showed a different pattern from that of Mokpo and Busan. After the summer, Bivalvia dominated on natural substrate over artificial substrates, affecting the differences between natural and artificial substrates. Our results demonstrate the recruitment patterns of sessile marine invertebrates according to substrate characteristics and can be used as basic information for biofouling management in marine environment.
Study of the EDM Process of Bimetallic Materials Using a Composite Electrode Tool

New types of profile products make complex use of bimetals. These materials possess a set of properties such as strength, corrosion resistance, thermal conductivity, heat resistance, wear resistance. For the processing of such products, it is advisable to use electrophysical processing methods, one of which is the technology of copy-piercing electrical discharge machining (EDM). Currently, EDM is one of the most common methods for processing products from modern bimetal materials. An urgent task is to study the EDM process of bimetallic materials. The aim of the work was to increase the efficiency and accuracy of the EDM process of bimetallic products using electrode-tools with different physical and mechanical properties. Bimetal—weld coated steel backing, base material—09G2S steel, surfacing material—M1 copper were used. The processing of the bimetallic workpiece was carried out on an Electronica Smart CNC copy-piercing EDM machine. EI used graphite, copper, and composite. A theoretical model was developed that allows calculation of the amount of removal of bimetallic material of steel–copper depending on the EDM modes and the ET (electrode tool) material. During the processing of the steel layer, regardless of the EI material, microcracks were formed along the grain boundaries, and during the processing of the copper layer, enlarged holes resulted.
Colorimetric and Electrochemical Methods for the Detection of SARS-CoV-2 Main Protease by Peptide-Triggered Assembly of Gold Nanoparticles

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease (Mpro) has been regarded as one of the ideal targets for the development of antiviral drugs. The currently used methods for the probing of Mpro activity and the screening of its inhibitors require the use of a double-labeled peptide substrate. In this work, we suggested that the label-free peptide substrate could induce the aggregation of AuNPs through the electrostatic interactions, and the cleavage of the peptide by the Mpro inhibited the aggregation of AuNPs. This fact allowed for the visual analysis of Mpro activity by observing the color change of the AuNPs suspension. Furthermore, the co-assembly of AuNPs and peptide was achieved on the peptide-covered electrode surface. Cleavage of the peptide substrate by the Mpro limited the formation of AuNPs/peptide assembles, thus allowing for the development of a simple and sensitive electrochemical method for Mpro detection in serum samples. The change of the electrochemical signal was easily monitored by electrochemical impedance spectroscopy (EIS). The detection limits of the colorimetric and electrochemical methods are 10 and 0.1 pM, respectively. This work should be valuable for the development of effective antiviral drugs and the design of novel optical and electrical biosensors.
Smart Electric Meter Deployment in Tanzania: A Survey

Using information and communication technologies (ICT) to make the electrical power network intelligent and smarter (smart grid) has been the focal point in transforming electrical power industry. The idea behind smart grid is to transform the Tanzanian power sector into a secure, adaptive, sustainable, and digitally enabled ecosystem that provides reliable and quality energy for all with active participation of stakeholders. Smart metering is a central segment in realizing smart grid. However, a big question is whether Tanzanian power stakeholders are ready for smart metering technology investments for household applications. Operation and maintenance of a smart metering solution is a relatively new business in Tanzania and requires investment in resources and capacity building. A case study was conducted at the utility company in Dar es Salaam offices, to investigate the deployment status and services offered. Fixed tariff rates, high cost, low rates on returns of investment and non-customization of the features, were some of the shortcomings identified by the study in terms of non-deployment in residential homes. Further, the authors, propose development of standardization document for smart metering technologies and the adoption of software based smart meter for residential applications using Internet of Things platform. Its low cost of development and ease installation would be ideal for residential applications. Keywords: Smart grid, Utility Company, Smart meter, Advanced Metering Infrastructure, Deployment Status.
Improved Performance of Solid Polymer Electrolyte for Lithium-Metal Batteries via Hot Press Rolling

Solid-state batteries (SSBs) are gaining attention as they promise to provide better safety and a higher energy density than conventional liquid electrolyte batteries. Solid polymer electrolytes (SPEs) are promising candidates due to their flexibility providing better interfacial contact between electrodes and the electrolyte. However, SPEs exhibit very low ionic conductivity at ambient temperatures, which prevents their practical use in batteries. Herein, a simple and effective technique of hot press rolling is demonstrated to improve ionic conductivity and, hence, the performance of polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP)-based solid polymer electrolyte. Applying hot press rolling to the electrolyte membrane induced structural changes in the grain boundaries, which resulted in a reduction in the crystallinity of the material and, hence, an increase in the amorphous phase of the material, which eased the movement of the lithium ions within the material. This technique also improved the surface of the membrane, making it homogeneous and smoother, which resulted in better interfacial contact between the electrodes and electrolyte. Electrochemical tests were carried out on electrolyte membranes treated with and without hot press rolling to evaluate the effect of the treatment. The hot pressed electrolyte membrane showed significant improvements in its ionic conductivity and transference number. The cycling performance of the LFP/Li batteries using a hot press rolled electrolyte was also evaluated, which gave a specific discharge capacity of 134 mAh/g at 0.1 C. These results demonstrate that hot press rolling can have a significant effect on the electrochemical performance of solid polymer electrolytes.
A Study for an Ideal Password Management System

Abstract: The growing number of online services needs users to have control over their password management system (generation, storage, recall). But the demand for total randomness and exclusivity of passwords is impractical in day-to-day life. Each component of a password management system comes with its cognitive burden on a user. There are many password management solutions available for users but every one of them has some drawbacks. Password managers have the ability to help users manage their passwords more successfully while also addressing many of the problems about password-based authentication. In this study, We're analyzing various previous studies regarding the effectiveness, usability, and security of password managers of all categories. Also, we're trying to come up with an ideal set of parameters to build the best possible password management system in 2022. This study will help to understand the key parameters and algorithms that we can use while building the ideal password generation, storage, and recall system for the user.
Difference in Pandemic-Related Experiences and Factors Associated with Sickness Absence among Nurses Working in COVID-19 and Non-COVID-19 Departments

Background: The aim of this study is to determine the main variables associated with nurses’ sickness absence (SA) and to improve the prediction of SA based on pandemic-related experiences. The second aim is to examine the differences between COVID-19 (CoV) and non-COVID-19 (non-CoV) nurses in levels of post-traumatic stress disorder (PTSD) symptoms, personality traits, coping strategies and professional stressors experienced. Methods: This historical prospective study enrolled 1305 nurses from the University Hospital of Split, Croatia. A total of 380 subjects participated in the study, 163 non-CoV and 217 CoV subjects. Nurses’ pandemic-related experience questionnaires, Big Five Inventory (BFI), Post-traumatic Stress Disorder Checklist (PCL-5), Coping Inventory for Stressful Situations (CISS) and Occupational Stress Questionnaire, were used for evaluation. Results: Non-CoV nurses felt more fear of infection, were more socially distanced, had more PTSD symptoms and neuroticism and felt more stress due to public criticism and job requirements compared to CoV nurses; p < 0.001. The groups of SA users and non-SA users could be distinguished based on predictor variables in CoV and non-CoV nurses, with a correct classification of 84.8% vs. 79.1%. Conclusions: It was possible to predict the probability of using SA among nurses due to pandemic professional experience, personality traits and coping strategies.
Linezolid Metabolism Is Catalyzed by Cytochrome P450 2J2, 4F2, and 1B1

The oxazolidinone antibacterial linezolid has been in clinical use for over 20 years, yet knowledge of the contributions of specific cytochrome (CYP) 450 enzymes to the metabolic clearance of this drug were mostly unknown. In this investigation, it was revealed that three P450 enzymes that had not been previously explored in linezolid metabolism, CYP2J2, CYP4F2, and CYP1B1, catalyzed the 2-hydroxylation and de-ethyleneation of the morpholine moiety of linezolid. The intrinsic clearance for linezolid metabolism in pooled human liver microsomes was low at 0.51 μL/min/mg protein, consistent with its in vivo clearance in humans, and the KM was high (>200 μM). In recombinant human P450 enzymes, a rank order of intrinsic clearance values for linezolid 2-hydroxylation were CYP2J2 ≫ CYP4F2 > CYP2C8 > CYP1B1 ≈ CYP2D6 ≈ CYP3A4 > CYP1A1 > CYP3A5, with nine other P450 enzymes showing no linezolid metabolism. The effect of selective inhibitors for these eight P450 enzymes on linezolid metabolism in pooled human liver microsomes was evaluated to provide estimates of the relative fractional contributions of these enzymes to linezolid metabolism. These experiments suggest that CYP2J2 and CYP4F2 contribute about 50% each to linezolid hepatic metabolism. It is proposed that the oxidative metabolic clearance of linezolid is primarily catalyzed by these two unusual P450 enzymes and that this explains the lack of observation of meaningful effects of common perpetrators of drug interactions on linezolid pharmacokinetics. SIGNIFICANCE STATEMENT Linezolid is an important antibacterial drug, but the enzymes involved in its oxidative metabolism were unknown. In this study, evidence is shown that supports an important role for two enzymes not frequently associated with the metabolism of drugs: cytochrome P450 2J2 and cytochrome P450 4F2. These observations offer insight to understand the results of clinical drug-drug interaction studies conducted on linezolid.
Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species

The investigation into promising botanical materials for natural cosmetics is expanding due to environmental and health awareness. Here, we aimed to evaluate the phytochemical substances and the potential skin-related pharmacological activities of four Mucuna seeds, namely M. gigantea (Willd.) DC. (MGG), M. interrupta Gagnep. (MIT), M. monosperma Wight (MMM), and M. pruriens (L.) DC. (MPR), belonging to the Fabaceae family. In methodology, the Mucuna seeds were authenticated using morphological and molecular approaches. L-DOPA, phenolics, and flavonoid content, incorporated with HPLC and GC–MS fingerprinting analyses, were determined. Then, skin-related antimicrobial, antioxidant, and antiaging activities were determined. The results revealed that MPR showed the highest L-DOPA content (75.94 mg/100 mg extract), whereas MGG exhibited the highest phenolic and flavonoid content (56.73 ± 0.62 mg gallic/g extract and 1030.11 ± 3.97 mg quercetin/g extract, respectively). Only MMM and MPR could inhibit all of S. aureus, S. epidermidis, and C. albicans, but no sample could inhibit C. acnes. Furthermore, all samples demonstrated antioxidant activity. Interestingly, all Mucuna samples exhibited strong collagenase, elastase, and hyaluronidase inhibitory activities. We conclude that the ethanolic extracts of four Mucuna seeds are probably advantageous in the development of skincare cosmeceutical products.
Normative values of the internal genital organs of the female pelvis in transvaginal and transabdominal ultrasound.

AIM To conduct a systemic review of published data on reference values for both transabdominal and transvaginal ultrasound in gynecology. MATERIALS AND METHODS Literature from 1970 to 2020 of reference values for the female pelvis in healthy subjects was reviewed. According to the determination of reference intervals for laboratory values reference values are generally determined using 95%-reference intervals and their associated 90%-confidence intervals. The list of articles was supplemented with extensive crosschecking of the reference lists of all retrieved articles. RESULTS A total of 33 studies were included and analyzed. The diagnostic performance of transvaginal ultrasound (TVUS) has a higher sensitivity and specificity than transabdominal ultrasound (TAUS) for high quality imaging of the uterus and the bilateral adnexa. The length of normal uterus is about 50-80 mm in fertile age. There is no consensus about the cut off value of the thickness of the endometrium in asymptomatic postmenopausal women, while a measurement of >5 mm and postmenopausal bleeding is suspect and requires further examination. The distribution of normal ovarian volumes is narrow with small volumes in postmenopausal women. CONCLUSION Normal values are helpful in delimiting the pathological changes in the female pelvis. While sonomorphologic criteria are more important than the ovarian size for the assessment of ovarian masses and reference values of the uterus in adults have little impact on routine practice, normative values in pediatric patients are important for the detection of pathologies. Normative values of the internal genital organs in females are sufficiently validated; still further research is required to assess the role of normative values in routine clinical practice and in sonographic screening for endometrial and ovarian cancer.
How Glutamate Promotes Liquid-liquid Phase Separation and DNA Binding Cooperativity of E. coli SSB Protein

E. coli single-stranded-DNA binding protein (EcSSB) displays nearest-neighbor (NN) and non-nearest-neighbor (NNN)) cooperativity in binding ssDNA during genome maintenance. NNN cooperativity requires the intrinsically-disordered linkers (IDL) of the C-terminal tails. Potassium glutamate (KGlu), the primary E. coli salt, promotes NNN-cooperativity, while KCl inhibits it. We find that KGlu promotes compaction of a single polymeric SSB-coated ssDNA beyond what occurs in KCl, indicating a link of compaction to NNN-cooperativity. EcSSB also undergoes liquid-liquid phase separation (LLPS), inhibited by ssDNA binding. We find that LLPS, like NNN-cooperativity, is promoted by increasing [KGlu] in the physiological range, while increasing [KCl] and/or deletion of the IDL eliminate LLPS, indicating similar interactions in both processes. From quantitative determinations of interactions of KGlu and KCl with protein model compounds, we deduce that the opposing effects of KGlu and KCl on SSB LLPS and cooperativity arise from their opposite interactions with amide groups. KGlu interacts unfavorably with the backbone (especially Gly) and side chain amide groups of the IDL and therefore promotes amide-amide interactions in LLPS and NNN-cooperativity. By contrast, KCl interacts favorably with these amide groups and therefore inhibits LLPS and NNN-cooperativity. These results highlight the importance of salt interactions in regulating the propensity of proteins to undergo LLPS.
Utilizing a Baculovirus/Insect Cell Expression System and Expressed Protein Ligation (EPL) for Protein Semisynthesis

Protein semisynthesis has been used for the chemoselective linking of synthetic peptides and recombinant protein fragments to generate complete native proteins in good yield. The ability to site‐selectively incorporate multiple post‐translational chemical modifications (PTMs) into proteins via this approach shows great potential for enhancing understanding of the molecular basis of protein function and regulation. Protein semisynthesis, however, often requires high expression efficiency of the recombinant protein fragments (i.e., high expression yield and ability to preserve protein biological functions), which can be hard to achieve for some human enzymes when using bacterial expression systems. Here, we describe how to use a baculovirus/insect cell expression system and a protein semisynthesis strategy known as expressed protein ligation (EPL) to produce workable levels of proteins of interest containing site‐specific chemical modifications. The protocol provides detailed guidance for generating protein C‐terminal thioesters for use with the EPL reaction, performing the EPL reaction, and purifying the protein ligation product. We exemplify the protocols by generating protein kinase Akt1 with site‐specific phosphorylations installed into its C‐terminal tail, for kinetic kinase assays. We hope these methods will help increase the use of protein semisynthesis for elucidating the post‐translational regulation of human enzymes involved in cell signaling. © 2022 Wiley Periodicals LLC
Inquest of Current Situation in Afghanistan Under Taliban Rule Using Sentiment Analysis and Volume Analysis

Microblogging websites and social media platforms serve as a potential source for mining public opinions and sentiments on a variety of subjects including the prevailing situations in war-afflicted countries. In particular, Twitter has a large number of geotagged tweets that make the analysis of sentiments across time and space possible. This study performs volume analysis and sentiment analysis using LDA (Latent Dirichlet Allocation) and text mining over two datasets collected for different periods. To increase the adequacy and efficacy of the sentiment analysis, a hybrid feature engineering approach is proposed that elevates the performance of machine learning models. Geotagged tweets are used for volume analysis indicating that the highest number of tweets is originated from India, the US, the UK, Pakistan, and Afghanistan. Analysis of positive and negative tweets reveals that negative tweets are mostly originated from India and the US. On the contrary, positive tweets belong to Pakistan and Afghanistan. LDA is used for topic modeling on two datasets containing tweets about the current situation after the Taliban take control of Afghanistan. Topics extracted through LDA suggest that majority of the Afghanistan people seem satisfied with the Taliban’s takeover while the topics from negative tweets reveal that issues discussed in negative tweets are related to the US concerns in Afghanistan. Sentiment analysis over two different datasets indicates that the trend of the sentiments has been shifted positively over three weeks.