Miracle-dz/UniFAR-FTUs huggingface dataset:
Improved clinical outcomes and physical activity in patients with rheumatoid arthritis treated with originator adalimumab in routine clinical practice

Background/Objective: We aimed at evaluating changes in habitual physical activity, disease activity and physical function following treatment with originator adalimumab (ADAORIG) in patients with Rheumatoid Arthritis (RA) in routine clinical practice. Methods: This observational study followed RA patients for 12 months, using the DAS28, HAQ-DI and Short QUestionnaire to ASsess Health-enhancing physical activity (SQUASH) instruments to measure disease activity, physical function and habitual physical activity, respectively. Associations of SQUASH scores with DAS28, HAQ-DI and socio-demographic factors were evaluated by correlation coefficients and multivariable regression analyses. Results: From baseline to final visit, the mean SQUASH total score increased from 4772 ± 4132 to 6104 ± 4921 (n=460), representing a 28% improvement in overall physical activity. Largest improvements were observed in the domains of leisure time and sport (42%), and activities at work and school (35%). Mean DAS28 and HAQ-DI scores decreased by 2.8 and 0.7 points, respectively, but a sizeable negative correlation was found only between baseline SQUASH total score and HAQ-DI score (–0.30; p<0.0001). Advancing age was negatively associated with the final SQUASH total score (effect estimate of –46.2, p<0.05). Conclusion: Improvements in habitual physical activity, disease activity, and physical function were observed in RA patients after 12 months of ADAORIG therapy.
Sobre el endemismo cantábrico Hieracium lainzii De Retz (Compositae) y especies afines = Cantabrian endemic Hieracium lainzii De Retz (Compositae) and related species

MATEO SANZ, G. (1996). Cantabrian endemic Hieracium lainzii De Retz (Compositae) and related species. Anales Jard. Bot. Madrid 54: 364-369 (in Spanish). Several species belonging to the Hieracium mixtum Froel, group growing in the Cordillera Cantabrica (N Spain) are discussed. A new species H. murlainzii Mateo is described and two new nomenclatural combinations, H. cantabrimontanum (De Retz) Mateo and H. pseudodulacianum (De Retz) Mateo, are proposed.
Influence of chlorpyrifos stress on protein metabolism of edible crab Barytelphusa guerini, and its recovery

The sublethal stress of chlorpyrifos on important metabolites and enzymes of protein metabolism was investigated in most important tissues (gills, muscle, hepatopancreas and nervous tissue) of freshwater edible crab, Barytelphusa guerini. The crabs were exposed to 0.07 mg/l (1/3 of LC 50 ) for 7, 14, 21 and 28 days. Subsequently, crabs were released into fresh water and kept in the same for 18 days in order to recovery study (3 days interval). Total protein content decreased whereas amino acids and ammonia increased in CPF exposed and maximum recovery was shown in cessation of intoxication. After cessation of intoxication recovery was observed. Urea content was decrease in all tissues and glutamine exhibited mixed response and recovery was highest in muscle. The activities of protease and aminotransferases were elevated in tissues for 28 days. Recovery of these enzymes activities was noticed during depuration. Acid phosphatase activity was inhibited in hepatopancreas and nervous ganglion and induced in gills and muscle. Alkaline phosphatase activity was enhanced in gills and hepatopancreas and inhibited in muscle and nervous ganglion. All these enzymes showed recovery after released of crabs into fresh water.
MARINE TROPHIC INTERACTIONS BY DYNAMIC SIMULATION OF FISH SPECIES

A mathematical model was developed for performing dynamic simulations of groups of interacting animal species. The energy balance of the individual animal was modeled so that growth and reproduction respond to food consumption after metabolic expenses are met. Populations change in response to recruitment (based on parental spawning) and mortality from natural causes, predation, starvation, and (where applicable) human exploitation. The forms of the various component mathema­ tical functions were derived from the available ecological sources. Functions and parameters are especially applicable to marine fish species. Trophic webs of any size or form can be constructed using this basic species model. Computer solution of the essentially continuous differential model gives a time history of trophic and population variables for all species in the web. Models of trophic webs of 2, 3, and 4 levels were constructed and exercised. These were used to examine effects of age class structure, reproductive time lag, and population regulation by starvation mortality and fecundity control. Competition between species and the effects of a top predator on competitors, with and without human exploitation, were studied. Thus far in the history of trophic ecology there has been little effort to bring together the important results of the diverse studies which provide the components of the total trophic system into a con­ struct that will permit analyzing the effects of metabolism, food consumption, reproductive ef­ fort, and the structure of the trophic' web upon the weight, population, and biomass of the various species involved. Perhaps the most complete and useful approaches in the literature are those of Menshutkin and Kislyakov (1967, 1968), Menshutkin (1968), Menshutkin and Prikhodko (1968, 1969, 1970), Karpov et al. (1969), Krogius et al. (1969), Menshutkin and Umnov (1970), Lassiter and Hayne (1971). The present work is an attempt to create a complete model for fish in the natural environment and to employ it for the stated type of total trophic analysis. The mathematical "trophic anatomy" of the generalized species modeled contains certain functions which represent trophic interactions with other species. The trophic web consists of an arbitrary number of such interacting species, coupled in this way into any arbitrary design; e.g., with any number of trophic "levels" (or couple~ across levels), any number of species at each level, any number of predator species on a single prey species, etc. The trophic properties of the
O mutante pso9-1 de Saccharomyces cerevisiae, sensível a psoralenos fotoativados, contém um alelo mutante do gene MEC3 envolvido em checkpoint

Complementation analysis of a pso9-1 yeast mutant sensitive to photoactivated monoand bifunctional psoralens, UV-light 254nm, and nitrosoguanidine, with pso1 to pso8 mutants, confirmed that it contains a novel pso mutation. Molecular cloning from a yeast genomic library suggested pso9-1 to be a mutant allele of the DNA damage checkpoint control gene MEC3. Noncomplementation of several sensitivity phenotypes in pso9-1/mec3∆ diploids verified allelism of both mutants. Sensitivity to calcofluor white and to caffeine was not increased in mec3∆ and pso9-1 backgrounds, in relation to their respective WTs, suggesting that pso9-1 does not carry a promoter mutation. The mutant phenotype of mec3∆ was slightly more pronounced for sensitivity to 8-MOP+UVA and UVC and for supression of UVC-induced forward mutation than that exhibited by the pso9-1 mutant, suggesting some residual function for the protein produced by this mutant allele.
Walking performances and muscle oxygen desaturation are increased after supervised exercise training in Takayasu arteritis: a case report and a review of the literature

Abstract Background Takayasu arteritis (TAK) is a rare chronic inflammatory vasculitis predominantly affecting the aorta and its main branches. Takayasu arteritis has been shown to increase cardiovascular risk. Supervised exercise training (SET) is a well-recognized and effective therapeutic tool improving walking performances in patients with chronic atherosclerotic disease; however, the effects of SET, and the underlying mechanisms, remain poorly documented in TAK patients. Case summary We reviewed the literature and investigated the effects of a 12-week SET programme on walking performances, physical function, and calf muscle oxygen saturation (StO2; assessed by near-infrared spectroscopy) during exercise in a 28-year-old man with TAK and symptoms of arterial lower limb claudication. The literature review evidences only two recent publications suggesting that exercise training is effective and well-tolerated in patients with arteritis. The treadmill pain-free (+22%) and maximal (+273%) walking distance, 6-min walking distance (+66%), and physical function of lower extremities (+20%) following SET were significantly improved in our patient. Moreover, we observed a greater muscle oxygen desaturation (increased oxygen extraction) during exercise. Discussion Following SET, the increased oxygen extraction may be related to improved microvascular milieu leading to a better match between muscle oxygen supply and demand during exercise. These new results may contribute to mechanistic insights in peripheral adaptations following exercise training in TAK patients and may help to explain, at least partly, the increased walking performances. Although more studies are needed to better explore the impact of exercise training, these results suggest that exercise should be recommended in TAK patients.
Heterologous overexpression, purification and mass spectrometric analysis of the human tachykinin NK2 receptor and expression of the human dopamine D2 receptor

103 INTRODUCTION 104 MATERIALS AND METHODS 106 Materials 106 Construction of the recombinant expression vector 106 Exchange of the rare codons present in the hNK2R for expression in E.coli 107 Expression of the MBP-hNK2R in E.coli 110 Analysis of the expression of the MBP-hNK2R fusion protein in E.coli 111 Purification of the MBP-hNK2R fusion protein 111 Cleavage of the MBP-hNK2R fusion protein and isolation of the hNK2R 112
Characterization of Ribosomal Protein L2 and Analysis of Missense Codon Discrimination in the Yeast Saccharomyces cerevisiae

Title of Document: CHARACTERIZATION OF RIBOSOMAL PROTEIN L2 AND ANALYSIS OF MISSENSE CODON DISCRIMINATION IN THE YEAST SACCHAROMYCES CEREVISIAE Johnathan Robert Russ, Ph.D., 2007 Directed By: Associate Professor Dr. Jonathan D. Dinman, Department of Cell Biology and Molecular Genetics Translational fidelity is of vital importance to the ribosome as it strikes an optimal balance between speed and accuracy. The study of factors contributing to the structure and function of the ribosome by assaying effects on fidelity yields important insights into the mechanism of the ribosome. Ribosomal protein L2 is vital to the structure and function of the ribosome with importance in RNA interaction and positioning, ribosomal subunit binding, and peptidyl transfer. Sixteen unique alleles of the RPL2A gene were studied for their effects on translational fidelity and general ribosome function. Mutations at V48D and L125Q of the highly structured globular domain proved generally deleterious to growth, but resistant to anisomycin, and markedly increased -1 programmed ribosomal frameshifting. H215Y, a mutation at the site of interaction with H93 of the peptidyl transferase center (PTC), was both sparsomycin and anisomycin resistant, indicating a functional impact on the PTC structure. Mutations at F185L and W195C border conserved rRNA binding regions and proved hyperaccurate in misincorporation studies. Understanding of fidelity and proper tRNA selection in translation requires analysis of how the ribosome discriminates between cognate, nearcognate, and non-cognate tRNAs. Measuring misreading of several codon substitutions in the context of paromomycin as well as mutations and deletions of contributing elongation factors and ribosome structural elements allowed further understanding of interactions governing tRNA selection and tRNA misincorporation events. A mutant of eEF1Bα, the nucleotide exchange factor for eEF1A, promoted a general increase in fidelity. Interestingly, a mutated ribosomal protein L5, though distant from the decoding center, also stimulated hyperfidelity in both nearand non-cognate cases. Distinction between nearand non-cognate tRNA interactions on the ribosome was observed in tests using paromomycin, and in studying mutations of eEF1Bγ with both demonstrating increased misreading of near-cognate codons but hyperaccurate decoding of non-cognate codons. CHARACTERIZATION OF RIBOSOMAL PROTEIN L2 AND ANALYSIS OF MISSENSE CODON DISCRIMINATION IN THE YEAST SACCHAROMYCES CEREVEVISIAE
Filament structure and phosphatase activity in the Rivulariaceae

A study was carried out on phosphatase activity, phosphate uptake and its relationship to hair formation in the Rivulariaceae. The Rivulariaceae was chosen as it is a widespread taxon, where hair formation is a common occurrence, and previous studies indicated that they originate from environments where a large proportion of the phosphorus (P) is present as organic P. It seems possible that hair-forming Rivulariaceae are especially well adapted to utilize organic P. Initially 51 axenic cyanobacterial strains, from 10 genera, were screened for yields using organic P sources and for cell-bound and extracellular phosphomonoesterase (PMEase) and phosphodiesterase (PDEase) activities. All strains exhibited detectable inducible PMEase activities, and highest cell-bound PMEase activities were in hair-forming Rivulariaceae. Synechococcus had significantly low cell-bound phosphatase activities and five strains were unable to hydrolyze phytic acid. PDEase activities were lower compared to PMEase activities in all strains. Strains isolated from deepwater rice habitats had significantly higher levels of PDEase activity. In the three Calothrix strains tested, Calothrix 202, 550 and 603, inducible phosphatase activities were similar whether the P source was inorganic or organic. PMEase synthesis in these strains began when cellular P (% dry wt) values were in the range 0.60 - 1.0%. Differences in the influence of environmental variables on cell-bound and extracellular PMEase activities in hair-forming Calothrix 550 were slight, suggesting that PMEases in the two fractions had a common origin. Of the eleven ions tested Ca had the most pronounced stimulatory effect on PMEase activity. Localization of enzyme activity in Calothrix 550 suggested that the enzyme was bound to a surface. Partial purification of an extracellular PMEase fraction detected four bands of PMEase activity on a non-denaturing polyacrylamide gel. Three of the four bands were associated with carbohydrate and the bands were not extractable by mechanical means. Localization of PMEase activity in hair-forming strains by azo dye (naphthol AS-MX) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) showed that PMEase activity was associated with hair cells. Phosphate uptake experiments with Calothrix 253 and 550 suggested that uptake at high external phosphate concentrations was located in hair cells. NaCl, above 67.5 mM, inhibited hair formation and subsequently phosphatase activity in Calothrix 253 and 690. Addition of mannitol or sorbitol had no effect on hair formation, suggesting inhibition of hair formation was not an osmotic effect. Removal of P-deficient cultures from saline to freshwater media led to a marked synchronization of hair formation (in 90% of trichomes) and increase in cell-bound PMEase activity. Localization of cell-bound PMEase activity by light microscopy, using naphthol AS-MX, detected activity in the hair cells.
BINARY LOGISTIC MODELING BACKWARD METHODS TO DETERMINE THE RISK FACTORS THAT AFFECT CERVICAL CANCER CASES

Background: Cervical cancer is second place as a killer of women in the world. Cervical cancer still be a problem of women's reproductive health. The incidence of both cervical cancer cases and deaths showed a trend that is increasing. Several factors are considered to influence the occurrence of cervical cancer are age, first marriage age, number of children, type of contraception, and length of contraception usage. This study aimed to analyze the influence of risk factors for cervical cancer to the cervical cancer cases in Wisnuwardhana’s Cancer Foundation in 2017 use binary logistic regression method. Materials and Methods: Based on the calculation of the sample size obtained case and control samples each of 40, so the total sample in this study as many as 80 samples. The method of determining and sampling is done by using simple random sampling method. The results were analysed using multivariate binary logistic regression modelling backward method. Result: The results of multivariate binary logistic regression showed five predictor variables were suspected of having links with cervical cancer cases, there are three variables, they are age (Pvalue = 0.010), first marriage age (Pvalue = 0.044) and type of contraception (Pvalue = 0.000) that significantly affect the cases of cervical cancer. Conclusion: The study revealed that age, first marriage age, and type of contraception are the significant risk factors associated with cervical cancer at Wisnuwardhana Cancer Foundation Surabaya.
Movement mysteries unveiled: spatial ecology of juvenile green sea turtles

Locations of important foraging areas are not well defined for many marine species. Unraveling these mysteries is vital to develop conservation strategies for these species, many of which are threatened or endangered. Satellite-tracking is a tool that can reveal movement patterns at both broad and fine spatial scales, in all marine environments. This chapter presents records of the longest duration track of an individual juvenile green turtle (434 days) and highest number of tracking days in any juvenile green turtle study (5483 tracking days) published to date. In this chapter, we use spatial modeling techniques to describe movements and identify foraging areas for juvenile green turtles (Chelonia mydas) captured in a developmental habitat in south Texas, USA. Some green turtles established residency in the vicinity of their capture and release site, but most used a specific habitat feature (i.e., a jettied pass) to travel between the Gulf of Mexico and a nearby bay. Still others moved southward within the Gulf of Mexico into Mexican coastal waters, likely in response to decreasing water temperatures. These movements to waters off the coast of Mexico highlight the importance of international cooperation in restoration efforts undertaken on behalf of this imperiled species.
Tree genetics and improvement

Tree improvement is a complex science and a multifaceted art. Tree improvement is most valuable when integrated within land-management programs. Major efforts are currently geared to developing improved seed for reforestation programs. Progeny testing is now a routine part of tree-improvement programs. Topics such as advanced-generation breeding, shortened generations, resistance breeding, and control of cone and seed insects are being given priority. Activity continues in basic research, involving genetic engineering, tissue culture, and population theory.
Two New Species of Vitronura (Collembola: Neanuridae) from Shanghai, Eastern China, with Dna Barcodes

ABSTRACT Vitronura setaebarbulata sp. nov. and V. quartadecima sp. nov. are described from Shanghai, China. V. setaebarbulata sp. nov. is characterized by its heavy barbulate macrosetae, very granulated tubercles, dorsointernal tubercles on head coalescent and very close each other, mandible with three big teeth, apical one with denticules and maxilla with one of the 2 lamellae trifurcated. V. quartadecima sp. nov. is characterized by having 14 tubercles on head, well isolated each other, smooth body setae and mandible with three big teeth, apical one with denticules. The COI barcode sequences were sequenced for four individuals of V. setaebarbulata sp. nov. and twelve individuals of V. quartadecima sp. nov. respectively. No genetic divergence between individuals of same species was found, and the divergence of nucleotides between the two new species is 18.54%. An updated key to world species of Vitronura is also provided.
Outpatients’ Satisfaction with the Provision of Physiotherapy Services

Background: Patient satisfaction is one of the indicators of the quality of care received by patients, which can be used as a benchmark to ensure quality of physiotherapy services. However, there is a dearth of data in Nigeria. Objectives: The current study aimed at examining the level of patients’ satisfaction with the physiotherapy services in Maiduguri, Nigeria. Methods: The current cross sectional study was conducted on a total of 300 participants (158 males and 142 females) randomly recruited from the physiotherapy outpatient unit of three hospitals. The physical therapy satisfaction questionnaire was administered to assess the participants’ level of satisfaction with the rendered physiotherapy services. Results: The overall patient satisfaction score was 82.95 ± 14.71. Privacy was the highest satisfactory subscale reported by the participants (83.50 ± 11.21), while facility locations/proximity (68.51 ± 13.20) and bills/cost (70.24 ± 19.40) were the least satisfactory subscales among the others. The result shows that female and old adult participants were significantly more satisfied with the received physiotherapy services compared with the male and young adult participants (P = 0.03 and 0.001, respectively). However, participants did not differ significantly in the level of satisfaction with provided physiotherapy services in terms of their conditions/ailments (P = 0.056). Conclusions: Overall, patient satisfaction with physiotherapy services in the current study was high. Females, older adults, as well as employed participants were more satisfied. Also, higher satisfaction was observed among participants with primary school education and the ones receiving multiple treatment modalities.
Supportive care needs of people with pancreatic cancer: a literature review

Quality of life is crucial to people with pancreatic cancer due to poor survival rates and high symptom burden. Timely and person-centred supportive and palliative care can help people maintain a good quality of life. This article reports findings of a review that highlights the physical and psychological needs of people with pancreatic cancer and interventions that may help meet those needs. Information, supportive and palliative care needs should be assessed, and appropriate interventions implemented early in the illness and on an ongoing basis. The article also highlights the role of a multidisciplinary team, including clinical nurse specialists, oncologists, palliative care specialists, dietitians and mental health practitioners, in implementing personalised care.
Response of predominant soil bacteria to grassland succession

The activity of uncultured bacteria, represented by five cloned ribotypes of the Holophaga/Acidobacterium-cluster, was monitored in Dutch grassland soils by quantifying the numbers of their ribosomes g" soil. The importance of these bacteria was indicated by the relative abundance of 16S rRNA RT-PCR products in temperature gradient gel electrophoresis fingerprints. The levels of rRNA in soil samples were quantified with multiple competitive RT-PCR along a 1.5 km transect through the grassland. In total, five members of the Holophaga/Acidobacterium-cluster were estimated to contribute 4 x 10 1 x 10 ribosomes gsoil, representing 7 14% of all bacterial ribosomes. These results indicate that ribosomes from Holophaga/Acidobacterium-cluster are homogeneously distributed in soil and might contribute significantly to microbial activity in soil.
The Role of Erythrocytic miRNA in the lifecycle of Plasmodium falciparum

The Role of Erythrocytic miRNA in the lifecycle of Plasmodium falciparum by Gregory Michael LaMonte University Program in Genetics and Genomics Duke University Date:_______________________ Approved: ___________________________ Jen-Tsan Ashley Chi, Supervisor ___________________________ Bryan Cullen ___________________________ Jack Keene ___________________________ Chris Nicchitta ___________________________ J. Brice Weinberg An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the University Program in Genetics and Genomics in the Graduate School of Duke University 2012 Copyright by Gregory Michael LaMonte 2012