Estimation of Primary Metabolites and Antioxidant Activity of Tylophora Indica l . and nyctanthes-arbor-tritis

The present study was aimed for isolation and quantification of Primary Metabolites from selected plant species. Antioxidant activity of plants was also carried out by using FRAP, Catalase, Lipid Peroxidation and Peroxidase assay. The maximum amount of primary metabolite was protein and the minimum was total soluble sugars in leaves of Tylophora indica whereas in the case of Nyctanthes arbor tritis. Maximum amount of primary metabolite was protein in roots and the minimum starch was found in stem. Thus leaves of Tylophora indica. and the roots of Nyctanthes arbor tritis were the rich source of protein. Stem of Tylophora indica was found to be better antioxidant as compared to leaves when observed by various antioxidant assays. In the case of Nyctanthes-arbortritis different plant parts showed potent activity when assayed by different methods. The present results showed potential of this medicinal plant which can be used as herbal drug as therapeutic ventures in future aspects. © 2017 Elixir All rights reserved. Elixir Appl. Botany 111 (2017) 48642-48645 Applied Botany Available online at www.elixirpublishers. com (Elixir International Journal) Deepika Harit and R.A. Shrama / Elixir Appl. Botany 111 (2017) 48642-48645 48643 other soluble in chloroform. Its roots are composed of alkaloids, tannins and glucosides (Suresh et al., 2011). The flowers are used in Asian continent to provoke menstruation and also used as sedatives. Flowers are also used to treat ulcers (Sasmal et al., 2007). Oral administration of decoction of flowers ward off wind in the stomach, stimulate gastric secretions and improve expectoration from the lungs (Suresh et al., 2011). The decoction is also used in treatment of gout. The inflorescence is used in scabies and skin diseases. Flower juice is used to combat hairloss and baldness (Tuntiwachwuttiku et al., 2003). Leaves are used as diuretic, laxative and diaphoretic. Leaf juice also prevents children who are infected with roundworms and threadworms. These are also used in treatment of appetite, piles, liver disorders, chronic and malarial fever and rheumatism. Leaves are also recommended in dry cough and skin infections and also used in gynecological disorders (Nawaz et al., 2009). Seeds are used in treatment of scurvy and anthelminthics. The bark is used for treatment of bronchitis and snakebite. Its roots are used as anthelminthics (Narendhirakannan and Smeera, 2010). Materials and Methods Collection of plant Material Plant parts of Tylophora indica (leaves and stem) and Nyctanthes arbortristis L. (leaves , roots, flowers and stem) were collected from the fields at Jaipur and authenticated. The voucher (RUBL* No. 20304 for Tylophora indica L. and RUBL* No. 20864 for Nyctanthes arbortristis L. of experimental plant was deposited in the Herbarium of Department of Botany, University of Rajasthan, Jaipur. The plant parts were separated and washed thoroughly 2-3 times with running tap water and then air dried under shade after complete shade drying the plant material was powdered and used for phytochemical analysis. Quantification of Primary Metabolites Quantification of Primary Metabolites were carried out by using following methods of carbohydrates, proteins, lipids and phenols.