Miracle-dz/UniFAR-FTUs huggingface dataset:
Exposure to environmental bisphenol A inhibits HTR-8/SVneo cell migration and invasion

Environmental pollutants, such as bisphenol A (BPA) have recently been implicated in the development of adverse birth outcomes. However, the underlying teratogenic mechanisms remain unclear. We investigated the effects of BPA on the migration and invasion of human primary extravillous trophoblast HTR-8/SVneo cells. Our results indicated that BPA reduced cell migration and invasion. Moreover, it altered the ratio of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) by downregulating MMP-2 and MMP-9, and upregulating TIMP-1 and TIMP-2. Furthermore, BPA suppressed integrin β1, integrin α5, and vimentin. Interestingly, BPA-induced invasion was partially restored by G15, a membrane G-protein-coupled estrogen receptor 30 antagonist. We further revealed that 42 proteins were differentially expressed by mass spectrometry analysis, which could be divided into three categories based on gene ontology including biological process, cellular component, and molecular function. These results suggest that BPA reduces HTR-8/SVneo cell migration and invasion by downregulating MMP-2 and MMP-9, up-regulating TIMP-1 and TIMP-2, and suppressing adhesion molecules.
Depression and polypharmacy are risk factors for activity limitation in type 2 diabetes

Objectives Individuals with type 2 diabetes are at risk for deficits in instrumental activities of daily living (IADL: e.g., managing medication, preparing healthy meals). Understanding which demographic and health factors contribute to decreased frequency of IADL performance is especially important to prevent age-related functional decline in type 2 diabetes. This study examined the relationships among demographic and health variables and frequency of IADL performance in adults with type 2 diabetes. Methods For this cross-sectional study, we collected health, demographic, and activity information via self-report and electronic health record review from safety-net primary care patients with type 2 diabetes (n = 93). We used regression models to determine which factors were associated with IADL performance in three subscales (domestic, leisure/work, outdoor) and the overall score. Results Depression was associated with decreased frequency of IADL performance for domestic, leisure/work, and outdoor activities. Each additional daily medication was associated with decreased frequency of domestic and outdoor IADL performance. Discussion Depression and taking more daily medication were predictors of decreased IADL performance for adults with type 2 diabetes. Our findings suggest regular screening for depression and polypharmacy is important in the primary care of diabetes; especially as these are potentially modifiable risk factors.
Effects of quadriceps angle on patellofemoral contact pressure

Background An inappropriate Q angle may affect the biomechanics of the canine patellofemoral joint. Objectives The purpose of this study was to evaluate the effects of changes in quadriceps angle (Q angle) on patellofemoral joint pressure distribution in dogs. Methods Eight stifles were positioned at 45, 60, 75, 90, 105, and 120° of flexion in vitro, and 30% body weight was applied through the quadriceps. Patellofemoral contact pressure distribution was mapped and quantified using pressure-sensitive film. For the pressure area, mean pressure, peak pressure, medial peak pressure, and lateral peak pressure, differences between groups according to conditions for changing the Q angle were statistically compared. Results Increases of 10° of the Q angle result in increases in the pressure area (P = 0.04), mean pressure (P = 0.003), peak pressure, and medial peak pressure (P ≤ 0.01). Increasing the Q angle by 20° increases the pressure area (P = 0.021), mean pressure (P ≤ 0.001), peak pressure (P ≤ 0.01), and medial peak pressure (P ≤ 0.01) significantly, and shows higher mean (P ≤ 0.001) and peak pressures than increasing by 10°. Decreasing the Q angle increases the mean pressure (P = 0.013), peak pressure, and lateral peak pressure (P ≤ 0.001). Conclusions Both increases and decreases in the Q angle were associated with increased peak patellofemoral pressure, which could contribute to the overloading of the cartilage. Therefore, the abnormal Q angle should be corrected to the physiologically normal value during patellar luxation repair and overcorrection should be avoided.
Single-Cell RNA-seq of Human Myeloid-Derived Suppressor Cells in Late Sepsis Reveals Multiple Subsets With Unique Transcriptional Responses: A Pilot Study

Supplemental Digital Content is available in the text ABSTRACT Background: Increased circulating myeloid-derived suppressor cells (MDSCs) are independently associated with poor long-term clinical outcomes in sepsis. Studies implicate subsets of MDSCs having unique roles in lymphocyte suppression; however, characterization of these cells after sepsis remains incomplete. We performed a pilot study to determine the transcriptomic landscape in MDSC subsets in sepsis using single-cell RNAseq (scRNA-seq). Methods: A mixture of whole blood myeloid-enriched and Ficoll-enriched PBMCs from two late septic patients on post-sepsis day 21 and two control subjects underwent Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq). Results: We successfully identified the three MDSC subset clusters—granulocytic (G-), monocytic (M-), and early (E-) MDSCs. Sepsis was associated with a greater relative expansion of G-MDSCs versus M-MDSCs at 21 days as compared to control subjects. Genomic analysis between septic patients and control subjects revealed cell-specific and common differential expression of genes in both G-MDSC and M-MDSC subsets. Many of the common genes have previously been associated with MDSC proliferation and immunosuppressive function. Interestingly, there was no differential expression of several genes demonstrated in the literature to be vital to immunosuppression in cancer-induced MDSC. Conclusion: This pilot study successfully demonstrated that MDSCs maintain a transcriptomic profile that is immunosuppressive in late sepsis. Interestingly, the landscape in chronic critical illness is partially dependent on the original septic insult. Preliminary data would also indicate immunosuppressive MDSCs from late sepsis patients appear to have a somewhat unique transcriptome from cancer and/or other inflammatory diseases.
Contact patterns in the ankle joint after lateral ligamentous injury during internal rotation: A computational study

Lateral ankle instability, resulting from the inability of ankle ligaments to heal after injury, is believed to cause a change in the articular contact mechanics that may promote cartilage degeneration. Considering that lateral ligaments’ insufficiency has been related to rotational instability of the talus, and that few studies have addressed the contact mechanics under this condition, the aim of this work was to evaluate if a purely rotational ankle instability could cause non-physiological changes in contact pressures in the ankle joint cartilages using the ﬁnite element method. A finite element model of a healthy ankle joint, including bones, cartilages and nine ligaments, was developed. Pure internal talus rotations of 3.67°, 9.6° and 13.43°, measured experimentally for three ligamentous configurations, were applied. The ligamentous configurations consisted in a healthy condition, an injured condition in which the anterior talofibular ligament was cut, and an injured condition in which the anterior talofibular and calcaneofibular ligaments were cut. For all simulations, the contact areas and maximum contact pressures were evaluated for each cartilage. The results showed not only an increase of the maximum contact pressures in the ankle cartilages, but also novel contact regions at the anteromedial and posterolateral sections of the talar cartilage with increasing internal rotation. The anteromedial and posterolateral contact regions observed due to pathological internal rotations of the talus are a computational evidence that supports the link between a pure rotational instability and the pattern of pathological cartilaginous load seen in patients with long-term lateral chronic ankle instability.
Nucleo‐plastidic PAP8/pTAC6 couples chloroplast formation with photomorphogenesis

The initial greening of angiosperms involves light activation of photoreceptors that trigger photomorphogenesis, followed by the development of chloroplasts. In these semi‐autonomous organelles, construction of the photosynthetic apparatus depends on the coordination of nuclear and plastid gene expression. Here, we show that the expression of PAP8, an essential subunit of the plastid‐encoded RNA polymerase (PEP) in Arabidopsis thaliana, is under the control of a regulatory element recognized by the photomorphogenic factor HY5. PAP8 protein is localized and active in both plastids and the nucleus, and particularly required for the formation of late photobodies. In the pap8 albino mutant, phytochrome‐mediated signalling is altered, degradation of the chloroplast development repressors PIF1/PIF3 is disrupted, HY5 is not stabilized, and the expression of the photomorphogenesis regulator GLK1 is impaired. PAP8 translocates into plastids via its targeting pre‐sequence, interacts with the PEP and eventually reaches the nucleus, where it can interact with another PEP subunit pTAC12/HMR/PAP5. Since PAP8 is required for the phytochrome B‐mediated signalling cascade and the reshaping of the PEP activity, it may coordinate nuclear gene expression with PEP‐driven chloroplastic gene expression during chloroplast biogenesis.
The Influence of Colour Features on Seed Identification Using Machine Vision

Little studies have been done on morphology of medicinal plants seeds. This paper presents an automatic system for medicinal plant seed identification and evaluates the influence of colour features on seed identification. Six colour features (means of red, green and blue colours of the seed surface, as well as means of hue, intensity and saturation) were extracted by algorithm and applied as network input. Different combinations of colour features (one, two three, four, five and six colour features) were used to find out the most accurate combination for seed identification. Results showed that the six colour feature was the most accurate combination for seed identification (99.184% and 87.719% for training and test of neural network respectively). One colour feature had the lowest average accuracy values for seed identification (3.120% and 2.771%). In general, increasing the number of colour features increased the total average of accuracy values.
Food habits of red drum and spotted seatrout in a restored mangrove impoundment

Benthic resource utilization by, red drum (Sciaenops ocellatus) and spotted seatrout (Cynoscion nebulosus) was studied in a restored, mangrove-rimmed impoundment (Cabbagehead Bayou) of Upper Tampa Bay, Florida, and in a nearby, natural site of unaltered tidal regime (Double Branch Bay). Diets of fish captured from August 1990 to May 1992 were determined from stomach content analysis. Simultaneously, food availability was evaluated by sampling benthic macroinvertebrates, mobile decapods, and small fish. Red drum and spotted seatrout utilized the restored habitat 1 yr after it was opened to tidal influence. Both species also were collected in the natural mangrove. Although there were noted differences in benthic assemblages between the two sites, red drum and spotted seatrout exhibited flexibility in diet, feeding on abundant and accessible prey. The high abundance of microcrustacea, such as amphipods, on detritus accumulated in the restored habitat constituted a main food resource for both fish species. Major food items in the diet of small (<200 mm) red drum were amphipods, mysids, and nereid and arenicolid polychaetes. Large (200–590 mm) red drum fed on polychaetes, xanthid crabs, palaemonid shrimp, and small fishes. Spotted seatrout preyed primarily upon mysids, shrimp, and small fishes, and to a lesser extent, upon a nereid polychaete. Our findings on fish feeding in a restored mangrove impoundment indicated that the detrital-associated benthic community is utilized by reinvading fish within a short time period, suggesting that not only habitat but food resources were augmented by the reopening of this wetland.
A portable primary radar for general aviation

A detailed situation awareness of the local environment is essential for safe flight in General Aviation. When operating under Visual Flight Rules, eyesight is crucial for maintaining situation awareness and objects may be overlooked. Technical solutions such as Flarm have been sought, but they only work on a basis of co-operation: obstacles without the proper equipment are invisible. Recent developments in the field of radar technology, partly empowered by the demand for sensors for autonomous cars, have improved the size and power consumption of available hardware. Today, the hardware exists to build a portable primary radar system for situation awareness. In this paper the results are presented of efforts to build the first portable primary radar for general, which has to be lightweight, cheap and have a low power consumption. The focus in this paper is on the software design of such a radar system. The physical principles of radar sensing are described, as well as the scientific steps needed to provide situation awareness. The hardware and software for the radar are both built and tested, and the results of these tests are presented. A flight experiment is performed with a small aircraft flying past a stationary radar on a small hill. It is found that the radar is capable of detecting the aircraft up to a distance of at least 3 kilometers. 3D localization is performed and the location determined by the radar was on average 46 meters away from the aircraft position as measured by satellite navigation, relative to a total distance of about 1000 meters from the radar. A low-pass filter can be applied on the raw results in order to improve the location estimation further. Future research will focus on bringing the portable radar in motion while operating.
Scaffold-Mediated Gene Delivery for Osteochondral Repair

Osteochondral defects involve both the articular cartilage and the underlying subchondral bone. If left untreated, they may lead to osteoarthritis. Advanced biomaterial-guided delivery of gene vectors has recently emerged as an attractive therapeutic concept for osteochondral repair. The goal of this review is to provide an overview of the variety of biomaterials employed as nonviral or viral gene carriers for osteochondral repair approaches both in vitro and in vivo, including hydrogels, solid scaffolds, and hybrid materials. The data show that a site-specific delivery of therapeutic gene vectors in the context of acellular or cellular strategies allows for a spatial and temporal control of osteochondral neotissue composition in vitro. In vivo, implantation of acellular hydrogels loaded with nonviral or viral vectors has been reported to significantly improve osteochondral repair in translational defect models. These advances support the concept of scaffold-mediated gene delivery for osteochondral repair.
Impact of thermal annealing in forming gas on the optical and electrical properties of MoS2 monolayer

Technological applications involving 2D MoS2 require transfer of chemical vapor deposition (CVD) grown material from its original substrate and subsequent lithographic processes. Inevitably, those steps contaminate the surface of the 2D material with polymeric residues affecting the electronic and optical properties of the MoS2. Annealing in forming gas is considered an efficient treatment to partially remove such residues. However, hydrogen also interacts with MoS2 creating or saturating sulfur vacancies. Sulfur vacancies are known to be at the origin of n-doping evident in the majority of as-grown MoS2 samples. In this context, investigating the impact of thermal annealing in forming gas on the electronic and optical properties of MoS2 monolayer is technologically important. In order to address this topic, we have systematically studied the evolution of CVD grown MoS2 monolayer using Raman spectroscopy, photoluminescence, x-ray photoelectron spectroscopy and transport measurements through a series of thermal annealing in forming gas at temperatures up to 500 °C. Efficient removal of the polymeric residues is demonstrated at temperatures as low as 200 °C. Above this value, carrier density modulation is identified by photoluminescence, x-ray photoelectron spectroscopy and electrical characterization and is correlated to the creation of sulfur vacancies. Finally, the degradation of the MoS2 single layer is verified with annealing at or above 350 °C through Raman and photocurrent measurements.
Radioimmunoassay for progesterone, testosterone and estradiol-17.BETA. using 125I-iodohistamine radioligands.

125I標識ステロイドホルモン(Amersham社製)を用いてP,T, E2のRIA法の設定を試みた結果,簡便で感度,精度,特異性の優れた二抗体法による方法を確立するに至った。 具体的な操作は以下の通りである。 1) 緩衝液:0.01MPBS(第二抗体用),1%BSA•PBS(標識ホルモン用),1%NRSあるいはNSS•0.05MEDTA•PBS(第一抗体用)。 2) ステロイドホルモンの抽出:抽出はエチルエーテルによる一回抽出法を用い,カラム等による精製操作は省略した。 未知試料(ラットの末梢血の場合は,P用として0.5~5μ1,T用として2.5~50μ1, E2用として200~300μ1)を抽出用試験管(13×100mm)に取り,純水を加えて全量を500μ1とする。 これにエチルエーテルを2~3m1加えて約20秒間攪拌し,ステロイドホルモンを抽出する。 約5分間静置後,ドライアイス•エタノール槽に浸して水層部分を凍結し,エーテル層をデカンテーションにより測定用試験管(12×75mm)に移す。 抽出物の入った測定用試験管を加温,減圧,振動して乾固する。 標準溶液は,保存溶液から純水を用いて段階稀釈(0.625~640Pg/0.5ml)して抽出用試験管に取り,未知試料と同様の方法で抽出操作を行う。 3) ラジオイムノアッセイ:乾固した測定用試験管に125I標識ステロイド(4,000~5,000cpm/100μ1)と第一抗体(100μ1)を加えて約20秒間攪拌し,4°Cにて約24時間イソキュベート後,第二抗体(200μ1)を加えてさらに4°Cにて24時間インキュベートし,遠心分離(1,7009,30分間)して沈渣の放射活性を計測する。
Microstructure of Human Corpus Callosum across the Lifespan: Regional Variations in Axon Caliber, Density, and Myelin Content.

The myeloarchitecture of the corpus callosum (CC) is characterized as a mosaic of distinct differences in fiber density of small- and large-diameter axons along the anterior-posterior axis; however, regional and age differences across the lifespan are not fully understood. Using multiecho T2 magnetic resonance imaging combined with multi-T2 fitting, the myelin water fraction (MWF) and geometric-mean of the intra-/extracellular water T2 (geomT2IEW) in 395 individuals (7-85 years; 41% males) were examined. The approach was validated where regional patterns along the CC closely resembled the histology; MWF matched mean axon diameter and geomT2IEW mirrored the density of large-caliber axons. Across the lifespan, MWF exhibited a quadratic association with age in all 10 CC regions with evidence of a positive linear MWF-age relationship among younger participants and minimal age differences in the remainder of the lifespan. Regarding geomT2IEW, a significant linear age × region interaction reflected positive linear age dependence mostly prominent in the regions with the highest density of small-caliber fibers-genu and splenium. In all, these two indicators characterize distinct attributes that are consistent with histology, which is a first. In addition, these results conform to rapid developmental progression of CC myelination leveling in middle age as well as age-related degradation of axon sheaths in older adults.
The Differential Composition of Whey Proteomes in Hu Sheep Colostrum and Milk during Different Lactation Periods

Simple Summary To reveal the temporal variation of ovine whey protein after lambing and provide basic data for lamb feeding and feed product development, the differential proteomes of whey during the transition from colostrum to mature milk in Hu sheep were studied. A total of 52 differentially expressed protein spots were detected among milk samples from six time points after lambing, identifying 25 differentially expressed proteins. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed the differentially expressed proteins involved in multiple biological functions, especially in immunity. Most of the differential proteins were highly expressed in the first 7 d after lambing, and the expression level decreased to a minimum value at 56 d. Abstract Colostrum and milk proteins are essential resources for the growth and development of the newborns, while their kinds and amounts vary greatly during the lactation period. This study was conducted to better understand whey proteome and its changes at six lactation time points (0 d, 3 d, 7 d, 14 d, 28 d, and 56 d after lambing) in Hu sheep. Using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) technologies, a total of 52 differentially expressed protein spots (DEPS), corresponding to 25 differentially expressed proteins (DEPs), were obtained. The protein spots abundance analysis revealed that the proteins are the most abundant at 0 d after lambing. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to explore the biological functions of the DEPs. The biological process was mainly involved in localization, the single-organism process, the cellular process, and a series of immune processes. The cellular components engaged in the extracellular region were the cell, organelle, and membrane. The most prevalent molecular function was binding activity. In addition, the DEPs were involved in nine significant pathways, including the Hippo signaling pathway and Complement and coagulation cascades. These results intuitively presented the changes in Hu sheep whey proteins during a 56-d lactation period, and revealed potential biological functions of the DEPs, providing a scientific basis for early weaning.
Vaccinations and Chronic Diseases: Knowledge, Attitudes, and Self-Reported Adherence among Patients in Italy

The aims of this cross-sectional survey were to evaluate the knowledge, attitudes, and self-reported adherence to recommended vaccinations among a random sample of patients with chronic conditions presenting for a medical visit in out-patient clinics in Italy. Patients who were healthcare workers (HCWs), those with diabetes, those who had received information by Internet, physicians, and friends/relatives, and those who needed more information were more likely to know that the influenza vaccine is recommended for patients with chronic diseases. More than half (58.2%) and 8.9% self-reported to have received at least one recommended vaccination and more than one, respectively. Patients who believed that vaccine-preventable diseases (VPDs) were dangerous for them, those who had received information by physicians, and those who needed information were more likely to have received at least one recommended vaccination. This behavior was less likely in married patients, those who were worried about the side effects of the vaccines, and those who suffered from renal failure. The results highlight the need to implement effective vaccination programs in order to decrease the complication of VPDs in at-risk population.