Miracle-dz/UniFAR-FTUs huggingface dataset:
Rui Yi-end formation in mammalian cell lineages ′ Genome-wide maps of polyadenylation reveal dynamic mRNA 3 Material Supplemental

Post-transcriptional regulation, often mediated by miRNAs and RNA-binding proteins at the 3′ untranslated regions (UTRs) of mRNAs, is implicated in important roles in the output of transcriptome. To decipher this layer of gene regulation, it is essential to measure global mRNA expression quantitatively in a 3′-UTR-specific manner. Here we establish an experimental and bioinformatics pipeline that simultaneously determines 3′-end formation by leveraging local nucleotide composition and quantitatively measures mRNA expression by sequencing polyadenylated transcripts. When applied to purified mouse embryonic skin stem cells and their daughter lineages, we identify 18,060 3′ UTRs representing 12,739 distinct mRNAs that are abundantly expressed in the skin. We determine that ∼78% of UTRs are formed by using canonical A[A/U]UAAA polyadenylation signals, whereas ∼22% of UTRs use alternative signals. By comparing to relative and absolute mRNA abundance determined by qPCR, our RNA-seq approach can precisely measure mRNA fold-change and accurately determine the expression of mRNAs over four orders of magnitude. Surprisingly, only 829 out of 12,739 genes show differential 3′-end usage between embryonic skin stem cells and their immediate daughter cells, whereas the numbers increase to 933 genes when comparing embryonic skin stem cells with the more remotely related hair follicle cells. This suggests an evolving diversity instead of switch-like dynamics in 3′-end formation during development. Finally, core components of the miRNA pathway including Dicer, Dgcr8, Xpo5, and Argonautes show dynamic 3′-UTR formation patterns, indicating a self-regulatory mechanism. Together, our quantitative analysis reveals a dynamic picture of mRNA 3′-end formation in tissue stem cell lineages in vivo.
MS guidelines for the management of multiple sclerosis § Recommandations de la Société libanaise de neurologie pour la prise en charge de la sclérose en plaques

Objective. – The prevalence of multiple sclerosis (MS) in Lebanon is unknown, as there are no available or reliable epidemiological studies to date. The circumstances of Middle East countries are different from those of Europe and North America in terms of differential diagnoses and disease management. The aim of the conference is to establish guidelines for diagnosis, treatment, follow-up and management of patients with MS in Lebanon. Another objective is to discuss and participate in research projects based on epidemiology, clinical trials and more fundamental aspects of the disease in the future. Methods. – Under the authority of the Lebanese society of Neurology (LSN), a group of neurologists took the initiative to participate in this LSN MS committee with the purpose of establishing a consensus for the management of patients with MS, and under the supervision of a Coordinator (A.T.) designed by the LSN board. Results. – Diagnostic and therapeutic, follow-up and research recommendations were proposed with special emphasis on the specific needs and circumstances of Lebanon. The § Official document LSN website: http://www.lsneuro. org/. * Corresponding author. E-mail address : atourbah@chu-reims.fr, ayman.tourbah@psl.aphp. fr (A. Tourbah). 1 for the Lebanese Society of Neurology: LSN Board of administration (2010–2012) – email: lsneurology@hotmail.com – Fadi Abou-Mrad (President), Nabil Mohsen (Vice-President), Rabih Saad (Secretary), Samir Ammar (Treasurer), Members: Nabil Akkawi, Elie Assaf, Michel Available online at www.sciencedirect. com Ferzli, Georges Hanna, Mohammed Kassem. 0035-3787/$ – see front matter # 2013 Elsevier Masson SAS. All rights reserved. doi:10.1016/j.neurol. 2012.12. 002
Designing pedagogical innovation for collaborating teacher teams

Abstract In this design-based research project, teachers co-created and used a new learning design model, the IT-Pedagogical Think Tank Model for Teacher Teams. This continuous-competence-development method enabled teachers to collaborate and develop innovative-learning designs for students in a new hybrid synchronous video-mediated learning environment. The article presents the IT-Pedagogical Think Tank Model and investigates how this new community of practice was supported and cultivated in the educational institution. The study took place at VUC Storstrøm, Denmark, where teachers taught students attending a full-time, two-year, upper-secondary, general-education programme. The findings were that various platforms, tools and social frameworks supported the pedagogical innovative process and established the team as a professional community of practice in the organisation. The team’s identity was strengthened as it added value to the organisation by inviting other communities of practice from the organisation into collaborative competence-development processes. The team members acted as good examples of innovative learning designers and were able to heighten the level of sophistication in the community’s pedagogical discussions. The school administration’s provision of resources and support was found to be a key factor in successful implementation of the new team meetings.
Alcohol-exposed PD Postnatal Day PFC Prefrontal Cortex PLAE : Prenatal and Lactation Alcohol-exposed PLP Myelin Proteolipid Protein ROS Reactive Oxygen Species TLR Toll-like Receptor VLN

Alcohol binge drinking is on the increase in the young adult population, and consumption during pregnancy can be deleterious for foetal development. Maternal alcohol consumption leads to a wide range of long-lasting morphological and behavioural deficiencies known as foetal alcohol spectrum disorders (FASD), associated with neurodevelopmental disabilities. We sought to test the effects of alcohol on neuroimmune system activation and its potential relation to alcohol-induced neurodevelopmental and persistent neurobehavioural effects in offspring after maternal alcohol binge drinking during the prenatal period or in combination with lactation. Pregnant C57BL/6 female mice underwent a procedure for alcohol binge drinking either during gestation or both the gestation and lactation periods. Adult male offspring were assessed for cognitive functions and motor coordination. Early alcohol exposure induced motor coordination impairments in the rotarod test. Object recognition memory was not affected by maternal alcohol binge drinking, but Y-maze performance was impaired in preand early postnatal alcohol-exposed mice. Behavioural effects were associated with an upregulation of pro-inflammatory signalling (Toll-like receptor 4, nuclear factor-kappa B p65, NOD-like receptor protein 3, caspase-1, and interleukin-1b), gliosis, neuronal cell death and a reduction in several structural myelin proteins (myelin-associated glycoprotein, myelin basic protein, myelin proteolipid protein and myelin regulatory factor) in both the prefrontal cortex and hippocampus of adult mice exposed to alcohol. Altogether, our results reveal that maternal binge-like alcohol consumption induces neuroinflammation and myelin damage in the brains of offspring and that such effects may underlie the persistent cognitive and behavioural impairments observed in FASD. © 2017 Elsevier Ltd. All rights reserved.
A study on Trend of Korean Forestry ODA Allocation

Yoon, Jun–Young; Park, Gwan–Soo; Lee, Sang–Jin; Lee, Si–Young; Ohga, Shoji.February, 2014.A study on trend of Korean forestry ODA allocation,Article,[Fukuoka, Japan]Kyushu University
ITE-SPECIFIC REGULATION OF CAV2.2 CHANNELS BY PROTEIN

bstract—Cav2.2 high voltage-gated calcium channels are egulated by phorbol-12-myristae, 13-acetate (PMA) via Ser/ hr protein kinase C (PKC) phosphorylation sites in the I–II inker and C-terminus of the 1 2.2 subunit. Here we show hat PMA enhancement of Cav2.2 currents expressed in Xeopus oocytes can be blocked by inhibitors of PKC II or KC isozymes, as shown previously for Cav2.3 currents, nd that microinjection of PKC II or PKC isozymes in the ocytes expressing the WT Cav2.2 channels increases the asal barium current (IBa). The I–V plot shows a large inrease in current amplitude with PKC II and PKC isozymes ith only a small shift in the peak IBa in the hyperpolarizing irection. The potentiation of Cav2.2 currents by microinjecion of PKC II and PKC isozymes was not altered by the nhibition of G proteins with GDP S. The combination of sozyme specific inhibitors with previously generated Ser/Thr o Ala mutants of 1 2.2 subunit revealed that PKC II or PKC isozymes (but not PKC or ) can provide full enhancement hrough the stimulatory site (Thr-422) in the I–II linker but that KC is better at decreasing channel activity through the nhibitory site Ser-425. The enhancing effect of PKC II or at hr-422 is dominant over the inhibitory effect at Ser-425. njected PKC II also enhances Cav2.2 current when any of he potential stimulatory sites (Ser-1757, Ser-2108 and Ser132) are available in the C-terminus. PKC provides lesser nhancement with C-terminal sites and only with Ser-2108 nd Ser-2132. Sites Ser-1757 and Ser-2132, but not Ser-2108, re dominant over the inhibitory site Ser-425. Collectively, hese results reveal a hierarchy of regulatory sites in Cav2.2 hannels. Site-specific regulation by different PKC isozymes ay allow graded levels of channel activation and susceptiility or resistance to subsequent stimulatory events. © 2009 BRO. Published by Elsevier Ltd. All rights reserved.
Size sensitive phenomena in superconducting wires and single charge devices

Savolainen, Marko Size sensitive phenomena in superconducting wires and single charge devices Jyväskylä: University of Jyväskylä, 2004, 181 p. (Research report/Department of Physics, University of Jyväskylä, ISSN 0075-465X; 4/2004) ISBN 951-39-1944-7 diss. In studying microor nanoscale phenomena, it is important to be able to routinely fabricate nanostructures, and particularly in statistical studies to fabricate a batch of similar structures. The main part of this thesis presents first results obtained by applying a novel ion beam sputtering method developed in this work to decrease dimensions of prefabricated nanostructures. The material is removed from the sample surface by bombarding it with energetic argon ion beam. The applicability of the method was tested with aluminium nanowires and single electron transistors and the promising results are presented in this thesis. An interesting feature of the method is that it can be applied multiple times so that dimensions are reduced gradually. This enables in general measurements of size-sensitive phenomena without fabricating numerous samples. This helps in ruling out circumstantial factors in fabrication. Also transport phenomena in single charge devices are discussed. Single electron transistors were used as electrometers in detecting tunnelling of electrons and Cooper pairs to an isolated piece of metal. For this purpose, special multilayer technique was applied in order to capacitively couple the systems. The effect of externally applied microwave signal to the current through single electron transistor was measured and compared with the theory. Also measurements and theoretical aspects of Cooper pair pumps are discussed.
Comparison of Hba 1 c Levels in Non-Diabetic Healthy Subjects and Subjects With Periodontitis Before and After Non-Surgical Periodontal Therapy

Methods: This comparative study was done on non-diabetic subjects ≥35 to ≤65 years old. Group A consisted of 30 subjects without periodontitis, and Group B consisted of 30 subjects with periodontitis. Body mass indexes (BMI) and clinical parameters, including oral hygiene index-simplified (OHI-S), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), and HbA1c levels, of all subjects were recorded. All subjects received non-surgical, periodontal therapy (scaling and root planning). After three months, all subjects were reexamined and clinical parameters and HbA1c levels were evaluated and compared with the baseline values.
2 ANNUAL REVIEW OF NURSING RESEARCH PTSD and the Veteran ’ s era of service

Posttraumatic stress disorder (PTSD) is an anxiety disorder that develops following exposure to a traumatic event. The prevalence and symptom severity of PTSD is greater in military combat Veterans than the civilian population. Although PTSD is a psychiatric disorder, in Veterans, it is associated with several physical comorbidities, chronic pain, substance abuse, and worse self-reported health status which may predispose them to greater perioperative morbidity and mortality. At present, the effect of surgery on the severity of PTSD is largely unknown. However, the perioperative clinician should consider PTSD a chronic illness associated with the accumulation of risk factors across the life span. POSTTRAUMATIC STRESS DISORDER Posttraumatic stress disorder (PTSD) is an anxiety disorder characterized by unwanted recurrent memories, avoidant behavior, physiological reactivity, emotional numbing, and intrusive thoughts that develop after exposure to a traumatic event and persist for at least 1 month (American Psychiatric Association, 2000). The lifetime prevalence of PTSD in the United States is estimated to be 6.8% (Kessler et al., 2005). The prevalence is greater in military Veterans, with 9%–43% demonstrating the disorder depending on the method used to diagnose ARNRv32_Final_A1_001-024.indd 1 8/29/14 12:23 PM 2 ANNUAL REVIEW OF NURSING RESEARCH PTSD and the Veteran’s era of service (Blake, Keane, et al., 1990; Dohrenwend et al., 2006; Hoge & Castro, 2006; Hoge et al., 2004; Vasterling et al., 2010). There are 20 recognized symptoms of PTSD, divided into four clusters (Table 1.1; American Psychiatric Association, 2013). In order for a patient to be diagnosed with PTSD, he or she must (a) have been exposed to a traumatic event which evoked intense feelings of fear, hopelessness, or horror; (b) demonstrate at least one clinically signifi cant symptom of intrusion, one clinically signifi cant symptom of avoidance, two clinically signifi cant symptoms of negative
Clinical Efficacy of Subgingival Debridement With Adjunctive Er : YAG Laser in Chronic Periodontitis Patients . A Randomised Clinical Trial

Materials and methods: In this 12-month single-masked, parallel group clinical trial, 40 moderate chronic periodontitis patients were selected and randomly assigned to a test treatment (consisting on one session of full-mouth ultrasonic subgingival debridement followed by Er:Yag application in sites with initial probing pocket depths of PD: ≥4.5 mm in another session one week apart), and a control group (with two sessions of ultrasonic debridement within one week). The main outcome variable was the changes in PD and, as secondary outcomes, changes in clinical attachment levels and proportions of bleeding on probing sites, which were assessed at baseline, 3, 6 and 12 months. Data was analysed with an intention to treat analysis, using ANOVA to assess for inter-group differences.
Balance in competition in Dutch soccer

We estimate an ordered probit model for soccer results in the Netherlands. The result of a game is assumed to be determined by home ground advantage and differences in quality between the opposing teams. The parameters of the model are used to assess whether the balance in competition in Dutch professional soccer has changed over time. Contrary to popular belief, we find that the balance has not changed much since the mid-1970s.
ENDINGS - ESPECIALLY REVERSAL ENDINGS

This article deals with different endings and especially with reversal endings. This kind of endings is of especial significance to the understanding of the work as a whole, because they cast it in a new or different light. The article contains three examples of reversal endings: the story of Judah and Tamar (Gen. 38); the book of Judges; and psalm 104. It seems to me that the knowledge that sometimes the ending of a biblical unit – story, poem or an entire book – directs readers to re-examine and consider the whole sequence in the light of its concluding information, contributes to our interpretations and may even lead to novel and possibly contradictory ones.
Varhaiskasvatuslain uudistustyön valmistuttua varhaiskasvatuksen laadun tarkastelu on ajankohtaista osana lain kokonaisvaikutusten arviointia. Lakireformin vaikuttimena on ollut

The recent policy shift in the Finnish early childhood education and care (ECEC) services creates a need to reflect the reform’s effect on quality. The economically driven legislation reform impacts the key assett of the service, the teaching staff. As quality ECEC provision continues to be the government’s priority, it is time to revise on our view of the constructs of quality. This article highlights the concept of ‘quality' with a special focus on the early years teachers' role. It summarizes the recent research findings through a literature review of four international academic journals: Australasian Jounal of Early Childhood, Contemporary Issues in Early Childhood, Early Child Development and Care and Early Childhood Research Quarterly. The review provides an overview of the variety of quality indicators across the international research network. The results indicated that regardless of the perspective into quality, its provision is dependable upon pedagogically competent teachers, who at best offer children everything they need to develop into their best selves and make meaning of the world moment by moment. This task requires specific skills and competences from teachers and hence the results highlight a gap between research and the ECEC policy shift in Finland.
Using QEMU in timing estimation for mobile software development

We present our research on using QEMU as a platform for software development that targets mobile hand-held devices. For such devices, understanding the timing of software execution is essential for energy consumption estimation. Traditional cycle-accurate simulators are orders of magnitude slower than real hardware, and thus unsuitable for software developers. Our research shows that embedding an abstract hardware simulator within QEMU achieves simulation times and accuracy required for software development.
Adherence to a blended smoking cessation treatment

Blended face-to-face and web-based treatment is a promising way to deliver smoking cessation therapy. Little is known about adherence to blended treatment and since adherence have been shown to be a measure for treatment’s acceptability and a determinant for treatment’s effectiveness, this study explores adherence to a blended smoking cessation treatment (BSCT). Patients’ (n=75) data were analyzed to trace adherence to BSCT delivered at an outpatient smoking cessation clinic. In total, 18 patient activities were selected to assess adherence. The minimum degree of adherence to these activities, for patients who reported abstinence at 6 months after treatment initiation, was used as threshold for adherence to BSCT. Finally, predictors for being adherent were studied out of 33 person-, smoking- and health-related baseline characteristics. Patients reporting abstinence, adhered to at least 61% of BSCT activities. Applying a 60% threshold, 18% of the patients were classified as adherent to BSCT. Marital status and social modelling were independent predictors for adherence. Patients having a partner had 11-times higher odds of being adherent (OR=11.3; CI: 1.33-98.99; p=0.03). While for social modeling – graded from 0 (partner and friends are not smoking) to 8 (partner and nearly all friends are smoking) – each unit increase was associated with 28% lower odds of being adherent (OR=0.72; CI: 0.55-0.94; p=0.02). The current study is the first study exploring adherence to a blended face-to-face and web-based smoking cessation treatment (BSCT) based on a substantial group of patients. It revealed a rather low adherence rate to BSCT. Furthermore, adherence was predicted by two baseline characteristics pertaining to patients’ social environment.
The Journal of Toxicological Sciences

Plasma alkaline phosphatase (ALP) activity is frequently measured in toxicity studies. In the present study, we assessed the usefulness of a commercially available polyacrylamide-gel (PAG) disk electrophoresis kit used in humans (AlkPhor System, Jokoh Co., Ltd., Tokyo, Japan) for identifying plasma ALP isoenzymes in mice of the Crlj:CD1 strain (ICR mice), which are commonly used in toxicity studies. We also examined age-related changes in plasma ALP isoenzymes in ICR mice. Electrophoresis was performed according to the manufacturer’s instructions. In order to identify the origin of each ALP isoenzyme, in addition to plasma samples, tissue ALP extracts from the liver, bone and small intestine were treated with neuraminidase, anti-small intestinal ALP antibody, ALP inhibitor levamisole and/or wheat germ agglutinin (WGA). The kit revealed that main plasma ALP isoenzyme in intact ICR mice was bone-derived one, and it tended to decrease with age. On the other hand, liver-derived ALP isoenzyme greatly increased in plasma of cholestasis model mice induced by bile duct ligation. This model mouse had also a large molecular ALP detected in the stacking gel. This ALP was thought to be of intestinal origin because its activity remained even after levamisole inhibition. In addition, a minimum sample volume for sufficient resolution of plasma ALP isoenzymes was only 14 μl. The results of this study suggest that the present method is a useful tool for detecting plasma ALP isoenzymes in mice and that pre-treatment of plasma with neuraminidase and concomitant levamisole inhibition with another gel is applicable for the evaluation of organ toxicity.
An evaluation of commercial banks lending to the productive sectors: Evidence from Namibia

The primary objective of this study is to evaluate the commercial banks on lending to the productive sectors in Namibia. The problem that the study has identified is that the commercial banks are reluctant to lend to the productive sectors of the economy. Most of the deposits that are mobilised locally by the commercial banks are not used to develop the domestic economy. Commercial banks in Namibia do not see it as their business to lend to risky long-term investments which are the driving force of the economy. This study reviews the main barriers to credit allocation to the private sector, small and medium enterprises, and the productive sectors. It also examines the extent to which the expansion of financial institutions offices, and improved links between these and the informal financial sector, could help to fill the credit gap. The quantitative approach analyses the data from bank’s annual reports. Qualitative approach is used through semistructured interviews to get the relevant information required for the study. In addition to the issue of access, the study observed that the issue of collateral played a crucial role in SME access to bank credit. Commercial banks insist on the “strict” form of collateral (insurance, salary/income, investment guarantees) which the SMEs cannot afford. Several actions, policies and recommendations are suggested to address these obstacles. The study suggests that it is necessary to establish the Development Bank of Namibia to avail financial resources to productive sectors of the economy in order to propel the
Ambisonics. Part one: General system description

Ambisonics is a technology for surround-sound which aims specifically at not making four (or any other number) of loudspeakers audible as separate sources of sound. It is designed using appropriate engineering methods and psychoacoustic theory that has shown good predictive value to make best use of available channels of communication (two or more ), and of loudspeakers (a limitation often forgotten), to give stable and uncoloured acoustic images in any position, keeping the physical means of reproducing the sound as unobtrusive as possible. It claims wide freedom of recording methods and of source material, as well as protection of recorded material from obsolescence.