Miracle-dz/UniFAR-FTUs huggingface dataset:
THE EFFECT OF THE PCOMS IN STUDENT COUNSELING

The aims of the present study were to investigate the effect of implementing the Partners for Change Outcome Management System (PCOMS) in the Danish Student Counseling Service and to explore both between-condition moderators and within-condition predictors of outcomes. The study was a non-randomized controlled study, comparing the outcome of individual and group student counseling for 634 PCOMS clients to that of 740 clients having started treatment as usual (TAU) two years before the PCOMS data collection began. The primary outcome measure was the Global Severity Index on the Symptom Checklist 90Revised. Main analyses were conducted with multilevel models on the intention-to-treat sample. The results demonstrated no effect of the PCOMS compared to the TAU condition, neither for the primary outcome nor for the number of dropouts or clients experiencing deterioration. The PCOMS effect was not predicted by the counselors’ adherence to the PCOMS protocol. Because the counselor level explained less than 1% of the variance in outcome, the counselor factors (i.e., attitude to feedback, reactions to negative feedback, and deliberate practice) were not analyzed as predictors. In conclusion, this study does not align with previous studies finding a positive effect of the PCOMS in counseling settings. However, all previous studies relied on the PCOMS Outcome Rating Scale as the only measure of outcome, maybe indicating a measure-specific effect.
Sparse Group Match for Point Cloud Registration

This paper proposes a point groups-based algorithm for point cloud registration. Most of the existing algorithms align two point clouds globally; however, they are unsuitable when the overlapping ratio is low or the inputs do not have strong features. The high accuracy of matched points is conducive for a rigid transformation of point clouds. This study aims to determine the exact matching points to register point clouds. The proposed method is based on point groups that are resampled point clouds. Subsequently, we calculate the multiple average probability (MAP) for each point group and match them by a sparse representation. Finally, the coherent point drift (CPD) algorithm is used to register the matched point groups, and the same transformation is applied to register the point clouds. The experimental results show that in terms of robustness to noise and outliers, our algorithm can register point clouds with a low overlapping ratio.
Diagnosis of Hodgkin's Lymphoma Using Endobronchial Ultrasound-Guided Transbronchial Needle

Endobronchial ultrasound-guided transbronchial biopsy has emerged as an excellent tool in diagnosing lung cancer. However, its use to diagnose lymphoma has been questioned, since the gold standard for diagnosing lymphomas is an excisional biopsy of involved lymph nodes. However, the procedure is sometimes risky or difficult. Recent studies have been showing great results using endobronchial ultrasound-guided transbronchial needle aspiration when accompanied by immunohistochemistry and cytology. Here, we present a case of Hodgkin's lymphoma patient that was accurately diagnosed using endobronchial ultrasound-guided transbronchial needle aspiration.
Quantifying the Financial Value of Clinical Specialty Choice and Its Association With Competitiveness of Admissions

Background The factors influencing medical student clinical specialty choice have important implications for the future composition of the US physician workforce. The objective of this study was to determine the career net present values (NPVs) of US medical students’ clinical specialty choices and identify any relationships between a specialty’s NPV and competitiveness of admissions as measured by the US Medical Licensing Examination (USMLE) Step 1 scores. Methodology NPVs were calculated using the results of the 2019 Doximity Physician Compensation report, a survey of 90,000 physicians. Mean USMLE Step 1 scores for matched US allopathic seniors in the 2018 National Resident Matching Program were used as a measure of clinical specialties’ competitiveness of admissions. We calculated a composite measure of NPV and annual work-hours by dividing each specialty’s NPV by the reported average number of hours worked per year. Results In our analysis, orthopedic surgery had the highest NPV ($10,308,868), whereas family medicine had the lowest NPV ($5,274,546). Dermatology and plastic surgery had the highest mean USMLE Step 1 scores (249 for both), whereas family medicine had the lowest (220). Clinical specialties’ NPVs were positively associated with mean USMLE Step 1 scores (Pearson’s r = 0.82; p < 0.001). Conclusions In this study, we describe associations suggesting that medical students respond to financial incentives in choosing clinical specialties and that these decisions are mediated by USMLE Step 1 scores. This underscores the importance of titrating and aligning incentives to improve the allocation of medical students into clinical specialties.
The ‘magic’ of magic camp from the perspective of children with hemiparesis

Previous studies have examined the quantitative results of using an organized approach of teaching and learning magic tricks—a therapeutic magic camp—but set aside the qualitative aspect. The purpose of this qualitative study is to explore the interpretation of the lived experience of children with hemiparesis after they have participated in a therapeutic magic camp. Six children, aged between 11 and 14 years old, with hemiparesis who completed the magic camp program 3 months prior were invited to participate in an in-person individual semistructured interview. The interviews were audio-recorded and transcribed verbatim for content analysis. Results from the initial qualitative analysis yielded categories of enjoyment, positive social and learning experience from participation in the camp, increased confidence, and self-motivation to use the affected upper limb. These categories were grouped into two themes: “having fun with others while learning” and “helping my arm perform. ” Having fun with others while learning was an amalgamation of fun, and positive social and learning experience. Helping my arm perform reflected a sense of increased competence and self-belief, and a willingness and motivation of the children to attempt challenging daily activities. The lived experiences of children with hemiplegia who participated in an organized delivery of learning to perform magic tricks yield-ed positive outcomes. These themes are consistent with the limited literature that is available suggesting that further research is needed to confirm the efficacy of the therapeutic magic camp intervention.
Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome

Antibiotic resistance is an emerging threat to global health. Current treatment regimens for these types of bacterial infections are becoming increasingly inadequate. Thus, new innovative technologies are needed to help identify and characterize novel drugs and drug targets which are critical in order to combat multidrug-resistant bacterial strains. Bacterial efflux systems have emerged as an attractive target for drug design, as blocking their export function significantly increases the potency of administered antibiotics. However, in order to develop potent and tolerable efflux pump inhibitors with high efficacy, detailed structural information is required for both the apo- and substrate-bound forms of these membrane proteins. The emergence of cryo-electron microscopy (cryo-EM) has greatly advanced the field of membrane protein structural biology. It has significantly enhanced the ability to solve large multi-protein complexes as well as extract meaningful data from a heterogeneous sample, such as identification of several assembly states of the bacterial ribosome, from a single data set. This technique can be expanded to solve the structures of substrate-bound efflux pumps and entire efflux systems from previously unusable membrane protein sample preparations. Subsequently, cryo-EM combined with other biophysical techniques has the potential to markedly advance the field of membrane protein structural biology. The ability to discern complete transport machineries, enzymatic signal transduction pathways, and other membrane-associated complexes will help us fully understand the complexities of the membrane proteome.
Maniraptoran pelvic musculature highlights evolutionary patterns in theropod locomotion on the line to birds

Locomotion is a fundamental aspect of palaeobiology and often investigated by comparing osteological structures and proportions. Previous studies document a stepwise accumulation of avian-like features in theropod dinosaurs that accelerates in the clade Maniraptora. However, the soft tissues that influenced the skeleton offer another perspective on locomotory adaptations. Examination of the pelvis for osteological correlates of hind limb and tail musculature allowed reconstruction of primary locomotory muscles across theropods and their closest extant relatives. Additionally, the areas of pelvic muscle origins were quantified to measure relative differences within and between taxa, to compare morphological features associated with cursoriality, and offer insight into the evolution of locomotor modules. Locomotory inferences based on myology often corroborate those based on osteology, although they occasionally conflict and indicate greater complexity than previously appreciated. Maniraptoran pelvic musculature underscores previous studies noting the multifaceted nature of cursoriality and suggests that a more punctuated step in caudal decoupling occurred at or near the base of Maniraptora.
Analysis of Immune Gene Expression Subtypes Reveals Osteosarcoma Immune Heterogeneity

Background Osteosarcoma (OS) patients have a poor response to immunotherapy due to the sheer complexity of the immune system and the nuances of the tumor-immune microenvironment. Methodology. To gain insights into the immune heterogeneity of OS, we identified robust clusters of patients based on the immune gene expression profiles of OS patients in the TARGET database and assessed their reproducibility in an independent cohort collected from the GEO database. The association of comprehensive molecular characterization with reproducible immune subtypes was accessed with ANOVA. Furthermore, we visualized the distribution of individual patients in a tree structure by the graph structure learning-based dimensionality reduction algorithm. Results We found that 87 OS samples can be divided into 5 immune subtypes, and each of them was associated with distinct clinical outcomes. The immune subtypes also demonstrated widely different patterns in tumor genetic aberrations, tumor-infiltrating, immune cell composition, and cytokine profiles. The immune landscape of OS uncovered the significant intracluster heterogeneity within each subtype and depicted a continuous immune spectrum across patients. Conclusion The established five immune subtypes in our study suggested immune heterogeneity in OS patients and may provide optimal individual immunotherapy for patients exhibiting OS.
Planarization of Substrate Topography by Spin Coating

The results of a study of topographic substrate planarization with films applied by a spin‐coating process are reported. It is shown that spin coating produces conformal film profiles over topographic gaps on the substrate that are wider than about 50 μm and that leveling of these gaps can only occur after spinning ceases if the film is able to flow over large distances. A comparison of the major forces acting on the film leads to the conclusion that the flow is driven primarily by capillarity when the width of the gap is less than 5000 μm. A theory is developed that relates the time required to level the gaps to their width and to the thickness and viscosity of the film. The results of experiments performed to test the theory are presented and discussed.
Computer modelling of the 4-n-alkyl-4′-cyanobiphenyls adsorbed on graphite: energy minimizations and molecular dynamics of periodic systems

The structures adopted within adsorbed monolayers of 4-n-octyl-4′-cyanobiphenyl (8-CB) molecules have been investigated using energy minimizations and molecular dynamics simulations of periodic systems. Using a smooth substrate potential, the most favourable energy of adsorption is found for a system with an eight-molecule unit-cell structure. This result is entirely consistent with scanning tunnelling microscopy studies of such systems, and differs from previous results using simulations of short strips which suggested a four-molecule unit cell. Molecular dynamics simulations of this 8-CB monolayer show that while the system exhibits smectic ordering at 150 K, the detailed eight-molecule unit-cell structure is lost. Simulations performed on a bilayer system indicate that the presence of a second molecular layer stabilizes the unit cell structure, except in the regions where there is partial penetration by the second layer molecules into the first layer. A third set of molecular dynamics simulations where.
A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations

A simple, fast, and validated HPLC method was developed for the simultaneous quantization of five cardiovascular agents: dopamine (DPM), dobutamine (DBM), phentolamine (PTM), furosemide (FSM), and aminophylline (APL) either in infusion samples or in an injection dosage form. The proposed method was achieved with a 150 mm × 4.6 mm, 5.0 μm C18 column, by using a simple linear gradient. Mobile phase A was buffer (50 mM KH2PO4) and mobile Phase B was acetonitrile at a flow rate of 1.0 mL/min. The column temperature was kept at 30°C, and the injection volume was 20 μL. All analytes were separated simultaneously at a retention time (tr) of 3.93, 5.84, 7.06, 8.76, and 9.67 min for DPM, DBM, PTM, FSM, and APL, respectively, with a total run time of less than 15.0 min. The proposed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. Linearity was obtained over a concentration range of 12.0–240.0, 12.0–240.0, 20.0–200.0, 6.0–240.0, and 10.0–200.0 μg/mL DPM, DBM, PTM, FSM, and APL, respectively. Interday and intraday accuracy and precision data were recorded in the acceptable limits. The new method has successfully been applied for quantification of all five drugs in their injection dosage form, infusion samples, and for evaluation of the stability of investigated drugs in mixtures for endovenous use. The results of the stability study showed that mixtures of DPM, DBM, PTM, FSM, and APL in 5% glucose or 0.9% sodium chloride injection were stable for 48 hours when stored in polypropylene syringes at 25°C.
Leaf litter identity rather than diversity shapes microbial functions and microarthropod abundance in tropical montane rainforests

Abstract In tropical forest ecosystems leaf litter from a large variety of species enters the decomposer system, however, the impact of leaf litter diversity on the abundance and activity of soil organisms during decomposition is little known. We investigated the effect of leaf litter diversity and identity on microbial functions and the abundance of microarthropods in Ecuadorian tropical montane rainforests. We used litterbags filled with leaves of six native tree species (Cecropia andina, Dictyocaryum lamarckianum, Myrcia pubescens, Cavendishia zamorensis, Graffenrieda emarginata, and Clusia spp.) and incubated monocultures and all possible two‐ and four‐species combinations in the field for 6 and 12 months. Mass loss, microbial biomass, basal respiration, metabolic quotient, and the slope of microbial growth after glucose addition, as well as the abundance of microarthropods (Acari and Collembola), were measured at both sampling dates. Leaf litter diversity significantly increased mass loss after 6 months of exposure, but reduced microbial biomass after 12 months of exposure. Leaf litter species identity significantly changed both microbial activity and microarthropod abundance with species of high quality (low C‐to‐N ratio), such as C. andina, improving resource quality as indicated by lower metabolic quotient and higher abundance of microarthropods. Nonetheless, species of low quality, such as Clusia spp. , also increased the abundance of Oribatida suggesting that leaf litter chemical composition alone is insufficient to explain variation in the abundances of soil microarthropods. Overall, the results provide evidence that decomposition and microbial biomass in litter respond to leaf litter diversity as well as litter identity (chemical and physical characteristics), while microarthropods respond only to litter identity but not litter diversity.
Second ACL Injury Rates in Younger Athletes Who Were Advised to Delay Return to Sport Until 12 Months After ACL Reconstruction

Background: Younger patients are at increased risk for anterior cruciate ligament (ACL) graft rupture and contralateral injury after ACL reconstruction (ACLR). Increasing the amount of time between surgery and the resumption of competitive sport may reduce this risk. Purpose: To determine the rates of graft rupture and injury to the contralateral native ACL at 3- to 5-year follow-up in younger patients who were advised to delay a return to competitive sport until 12 months after surgery and compare this with a nondelayed cohort. Study Design: Cohort study; Level of evidence, 3. Methods: The primary study cohort consisted of 142 eligible patients aged <20 years when they underwent their first primary ACLR. All were informed about the risk of further injury and advised not to return to competitive sport before 12 months postoperatively. Return-to-sport status and the number of subsequent ACL injuries (graft rupture or a contralateral injury to the native ACL) were determined at 3- to 5-year follow-up and compared with a historic cohort of 299 patients with ACLR who were not advised to delay their return to sport for a set period. The cohorts were then combined, and injury rates were compared between those who returned to sport before and after 12 months postoperatively. Results: The follow-up rate was 91% (129/142). In the delayed cohort, 63% returned to competitive sport after 12 months (mean, 14 months). Few patients (n = 10) returned before 9 months. In the delayed group, 33% had a subsequent ACL injury; this was not significantly different when compared with the nondelayed group (31% rate), in which a majority (58%) returned to competitive sport before 12 months. Subsequent ACL injury rates were also similar when compared between patients who returned before and after 12 months postoperatively (33% vs 32%, respectively). Conclusion: At midterm follow-up, the overall rates of subsequent ACL injury were high, even for patients who delayed their return until 12 months after surgery. More research is required to identify strategies to reduce the high reinjury rate in younger athletes.
Prophylactic Antibiotics for Urinary Tract Infections after Urodynamic Studies: A Meta-Analysis

Aim We aimed to perform a meta-analysis to determine whether antibiotic prophylaxis reduces the incidence of urinary tract infections (UTIs) after urodynamic studies (UDS). Methods We conducted a systematic search of PubMed, Web of Science, Ovid, Elsevier, ClinicalKey, Embase, Cochrane Library, Medline, and Wiley Online Library. Randomized controlled trials (RCTs) comparing the effectiveness of prophylactic antibiotics with placebo or no treatment in preventing UTI after UDS were included. Two reviewers extracted data independently, and RevMan 5.3 software was used to analyze relative risk (RR) with 95% confidence intervals (CI). Heterogeneity was assessed by the Q test and I2 test. Results The final meta-analysis included 1829 patients in 13 RCTs. Compared with the placebo or no treatment group, prophylactic antibiotics could significantly reduce the risk of bacteriuria (RR = 0.42, 95% CI: 0.30-0.60) and the risk of symptomatic UTI (RR = 0.65, 95% CI: 0.48-0.88). In addition, there was no statistically significant difference in the risk of adverse events (RR = 4.93, 95% CI: 0.61-40.05). No significant heterogeneity or publication bias was found in this study. Conclusions Current evidence showed that prophylactic antibiotics could reduce the risk of asymptomatic bacteriuria and symptomatic UTI after UDS without increasing the incidence of adverse events.
Antimicrobial Potential and Phytochemical Screening of Clathria sp. 1 and Tedania (Tedania) stylonychaeta Sponge Crude Extracts Obtained from the South East Coast of South Africa

Scientists have continuously searched for novel bioactive compounds to overcome the inherent problems associated with drug resistance, the evolution of unknown diseases, and the toxicity of currently used compounds. The ocean has been considered a rich source of compounds that possess unique chemical structures and novel biological capabilities. Biologically active molecules isolated from marine flora and fauna have shown significant advancement over the past century in the pharmaceutical industry. Marine natural products (MNPs) have been used as nanomedicine, cosmetics, wound healing, antimicrobial agents, anticancer agents, and anti-inflammatory agents. The physicochemical parameters of the collection site were also recorded. This study's marine sponge species were collected from Phillip's Reef, South Africa, at 12 m during the spring season. Ethyl acetate (EA) and dichloromethane : methanol (DCM : ME, 1 : 1) were used as extraction solvents. Crude extracts of the marine sponges were tested against MRSA, P. aeruginosa, C. difficile, A. fumigatus, and C. albicans. Phytochemical screening was conducted to identify seven critical phytochemical groups. A pH reading of 8.01 and a temperature of 15.45°C were recorded at the sampling site. Clathria sp. 1 and Tedania (Tedania) stylonychaeta EA crude extracts showed bioactivity against all five test pathogens. The DCM : ME crude extract of Clathria sp. 1 was the only bioactive crude extract from DCM : ME extracts. This crude extract was only bioactive against C. albicans as no activity was observed against the other four pathogens. EA crude extracts of Clathria sp. 1 yielded more significant inhibition zones against both fungal pathogens. These EA crude extracts performed better than fluconazole as inhibition zones of 35 ± 0 mm at 24 mg/ml, 31 ± 0 mm at 19 mg/ml, 31 ± 0 mm at 14.4 mg/ml, 30 ± 0 mm at 9.6 mg/ml, and 25 ± 0 mm at 7.2 mg/ml were recorded. Clathria sp. 1 crude extracts exhibited higher inhibition zones compared to Tedania (Tedania) stylonychaeta. The antibiotic imipenem (26 ± 0.7 mm at 10 μg) and ciprofloxacin (30 ± 0.3 mm at 5 μg) exhibited higher zones of inhibition than EA crude extracts of Tedania (Tedania) stylonychaeta at all test concentrations. In this study, Clathria sp. 1 was observed to have broad-spectrum bioactivity as EA crude extracts were bioactive against MRSA, P. aeruginosa, C. difficile, A. fumigatus, and C. albicans. In addition to this, the EA crude extract of Clathria sp. 1 was bacteriostatic (9.6 mg/ml). Clathria sp. 1 DCM : ME crude extract only tested positive for the presence of terpenoids. In contrast, EA crude extracts did not test positive for the existence of any of the seven phytochemicals. Our study has revealed that Tedania (Tedania) stylonychaeta and Clathria sp. 1 sponge species collected from Phillip's Reef in South Africa can produce bioactive compounds useful against bacterial and fungal species.