Miracle-dz/UniFAR-FTUs huggingface dataset:
An early Miocene dugongine (Sirenia: Dugongidae) from Panama

ABSTRACT Herein, we describe a new early Miocene dugongine from marine deposits of the Culebra Cut (Gaillard Cut) of the Panama Canal. The new taxon, Culebratherium alemani, gen. et sp. nov., represents one of the few records of late Aquitanian–early Burdigalian sirenians and the oldest sirenian from Central America. A phylogenetic analysis places Culebratherium in a clade with Dioplotherium cf. D. allisoni (Miocene of Brazil), Dioplotherium allisoni (Miocene of Baja California Sur, Mexico, and California, U.S.A.), and Dioplotherium sp. (Pliocene of Yucatan, Mexico). Similar to these taxa, Culebratherium is characterized by the presence of large incisor tusks, a premaxillary symphysis without a boss, a premaxilla-frontal suture forming a butt joint, and a moderately downturned rostrum. In addition, Culebratherium exhibits prominent occipital-cervical attachment sites for enlarged neck musculature. These features taken together are interpreted as adaptations for uprooting large, deeply buried seagrass rhizomes. Other dugongines with similar, yet convergent, dental and facial adaptations are known from earlier or coeval deposits in Puerto Rico, Florida, South Carolina, California, Baja California Sur, Brazil, and India and were constituents of sympatric paleocommunities of sirenians. Only fragmentary evidence of a second smaller and unidentifiable sirenian species is known from the Culebra Formation, but future discoveries may reveal a similar sympatric paleocommunity during the early Miocene of Panama. Finally, we used the results of the phylogenetic analysis to propose the new clade Pan-Sirenia as the most inclusive group consisting of stem and crown groups and redefine the Sirenia, Dugongidae, and Dugonginae clades.
Tracking genetic invasions: Genome‐wide single nucleotide polymorphisms reveal the source of pyrethroid‐resistant Aedes aegypti (yellow fever mosquito) incursions at international ports

Biological invasions are increasing globally in number and extent despite efforts to restrict their spread. Knowledge of incursion pathways is necessary to prevent new invasions and to design effective biosecurity protocols at source and recipient locations. This study uses genome‐wide single nucleotide polymorphisms (SNPs) to determine the origin of 115 incursive Aedes aegypti(yellow fever mosquito) detected at international ports in Australia and New Zealand. We also genotyped mosquitoes at three point mutations in the voltage‐sensitive sodium channel (Vssc) gene: V1016G, F1534C and S989P. These mutations confer knockdown resistance to synthetic pyrethroid insecticides, widely used for controlling invertebrate pests. We first delineated reference populations using Ae. aegypti sampled from 15 locations in Asia, South America, Australia and the Pacific Islands. Incursives were assigned to these populations using discriminant analysis of principal components (DAPC) and an assignment test with a support vector machine predictive model. Bali, Indonesia, was the most common origin of Ae. aegypti detected in Australia, while Ae. aegypti detected in New Zealand originated from Pacific Islands such as Fiji. Most incursives had the same allelic genotype across the three Vsscgene point mutations, which confers strong resistance to synthetic pyrethroids, the only insecticide class used in current, widely implemented aircraft disinsection protocols endorsed by the World Health Organization (WHO). Additionally, all internationally assigned Ae. aegypti had Vssc point mutations linked to pyrethroid resistance that are not found in Australian populations. These findings demonstrate that protocols for preventing introductions of invertebrates must consider insecticide resistance, and highlight the usefulness of genomic data sets for managing global biosecurity objectives.
Effect of different chelating agents on the bond strength of a silicone-based root canal sealer to root dentin

Aim: The purpose of this study was to evaluate the impact of 7% maleic acid (MA), 17% ethylenediaminetetraacetic acid (EDTA) solution, 10% citric acid (CA), or 2.25% peracetic acid (PAA) on the dislodgment resistance of a silicone-based root canal sealer. Materials and Methods: Ninety-five mandibular incisors were shaped to size R50. The specimens were randomly assigned to the following five groups (n = 15) based on the final irrigation solution: 5% sodium hypochlorite (NaOCl), 17% EDTA, 7% MA, 10% CA, and 2.25% PAA. Four specimens from each group were examined under scanning electron microscope. All the remaining canals were obturated with GuttaFlow® Bioseal. Bond strength was assessed using the push-out test. The data were analyzed statistically by two-way analysis of variance and Bonferroni tests at the significance level of P = 0.05. Results: The roots irrigated with chelators showed statistically significantly higher bond strength than the roots irrigated with NaOCl (P < 0.05). Overall, MA showed the highest bond strength, but there was no statistically significant difference among the other chelating solutions (P > 0.05). Conclusions: The bond strength of the GuttaFlow Bioseal to the root canal dentin may increase by the removal of smear layer. Final irrigation with the investigated chelators resulted in similar bond strength of the GuttaFlow Bioseal.
Evaluation of apical extrusion and cone-beam computed tomography assessment of irrigant penetration in oval-shaped canals, using XP Endo Finisher and EndoActivator

Background: Thorough cleaning of the pulp space is a challenging task. The mechanical instrumentation alone is usually not sufficient to completely debride the canals, and therefore, it requires the chemical action of irrigants also to disinfect the difficult to reach areas. Aim: The purpose of this study was to determine apical extrusion and assess irrigant penetration through cone-beam computed tomography (CBCT) for EndoActivator (EA) and XP Endo Finisher (XP). Materials and Methods: Sixty single-rooted mandibular premolars with oval-shaped canals were equally divided into three groups after instrumentation, based on the final irrigation: Group-1 syringe needle (30G Max-I-probe), Group-2 EA, and Group-3 XP. After the final irrigation, the weight of the extruded sodium hypochlorite was calculated. The prepared canals were then irrigated with a radiopaque contrast medium, which was activated according to the group of the sample (Group-1, 2, or 3). The volume of irrigant filled in the canal, especially in the apical third was determined through special tools in CBCT imaging. Statistics: One-way ANOVA test was used to compare the different groups. Results and Conclusion: Significantly more apical extrusion was seen in XP (P < 0.001). Both XP and EA have shown complete penetration of irrigant in the canal (100%).
Pulpal-anesthesia of a mandibular first molar with irreversible pulpitis by inferior alveolar nerve block plus buccal infiltration using articaine or lignocaine

Introduction: This study aims to compare the efficacy of a combination of an inferior alveolar nerve block (IANB) plus buccal infiltration using 4% articaine versus 2% lignocaine in achieving anesthesia of lower first molar teeth with irreversible pulpitis. Materials and Methods: Seventy adult patients were selected. A random sequence list was employed to administer IANB plus buccal infiltration. After the onset of lip numbness, cold test and electric pulp testing were performed. Five patients, four missed blocks and one no bleeding, were excluded. Heft Parker Visual Analog Scale scores during pulp extirpation were recorded. The data of sixty-five patients were statistically analyzed using Chi-square and Mann–Whitney U-test. Results: The success rate after lip-numbness for articaine is 91.42% and for lignocaine is 94.28%. The difference is statistically, not significant (P = 0.6425). During access, the success rate for lignocaine is 96.87%, whereas 96.96% for articaine. This difference is also not significant (P = 0.982366). Conclusion: IANB plus buccal infiltration using articaine or lignocaine is equally effective in anesthetizing mandibular first molar with irreversible pulpitis.
IgG Subclasses Shape Cytokine Responses by Human Myeloid Immune Cells through Differential Metabolic Reprogramming

Key Points Human IgG subclasses elicit distinct cytokine profiles by myeloid immune cells. Subclass-specific effects are mediated by distinct metabolic reprogramming by FcγRs. IgG subclasses provide pathogen- and cell type–specific immunity. IgG Abs are crucial for various immune functions, including neutralization, phagocytosis, and Ab-dependent cellular cytotoxicity. In this study, we identified another function of IgG by showing that IgG immune complexes elicit distinct cytokine profiles by human myeloid immune cells, which are dependent on FcγR activation by the different IgG subclasses. Using monoclonal IgG subclasses with identical Ag specificity, our data demonstrate that the production of Th17-inducing cytokines, such as TNF, IL-1β, and IL-23, is particularly dependent on IgG2, whereas type I IFN responses are controlled by IgG3, and IgG1 is able to regulate both. In addition, we identified that subclass-specific cytokine production is orchestrated at the posttranscriptional level through distinct glycolytic reprogramming of human myeloid immune cells. Combined, these data identify that IgG subclasses provide pathogen- and cell type–specific immunity through differential metabolic reprogramming by FcγRs. These findings may be relevant for future design of Ab-related therapies in the context of infectious diseases, chronic inflammation, and cancer.
The Interplay between Fe3O4 Superparamagnetic Nanoparticles, Sodium Butyrate, and Folic Acid for Intracellular Transport

Combined treatments which use nanoparticles and drugs could be a synergistic strategy for the treatment of a variety of cancers to overcome drug resistance, low efficacy, and high-dose-induced systemic toxicity. In this study, the effects on human colon adenocarcinoma cells of surface modified Fe3O4 magnetic nanoparticles (MNPs) in combination with sodium butyrate (NaBu), added as a free formulation, were examined demonstrating that the co-delivery produced a cytotoxic effect on malignant cells. Two different MNP coatings were investigated: a simple polyethylene glycol (PEG) layer and a mixed folic acid (FA) and PEG layer. Our results demonstrated that MNPs with FA (FA-PEG@MNPs) have a better cellular uptake than the ones without FA (PEG@MNPs), probably due to the presence of folate that acts as an activator of folate receptors (FRs) expression. However, in the presence of NaBu, the difference between the two types of MNPs was reduced. These similar behaviors for both MNPs likely occurred because of the differentiation induced by butyrate that increases the uptake of ferromagnetic nanoparticles. Moreover, we observed a strong decrease of cell viability in a NaBu dose-dependent manner. Taking into account these results, the cooperation of multifunctional MNPs with NaBu, taking into consideration the particular cancer-cell properties, can be a valuable tool for future cancer treatment.
Comparative evaluation of clinical performance of two self-etch adhesive systems with total-etch adhesive system in noncarious cervical lesions: An in vivo study

Background: The growing demands for esthetic restorations have stimulated intensive research in the field of adhesive dentistry. Dental adhesive systems are used to promote adhesion between composite resins and dental structure. In the fundamental principles of adhesion, the primary mechanism contributing to the formation of adhesion is micromechanical bonding between the restoration and the tooth. The bond strength of self-etching adhesives to dentin was found to be almost equal to that of total-etch adhesives. The aim of the present prospective, double-blind, randomized controlled clinical trial was to evaluate and compare the clinical performance of two self-etch adhesive systems with total-etch adhesive system in noncarious cervical lesions (NCCLs). Materials and Methods: In each patient, three teeth were randomly assigned according to the adhesive system used to Group A (total-etch adhesive system), Group B (two-bottle self-etch adhesive system), and Group C (one-bottle self-etch adhesive system). The clinical efficacy of these adhesive systems was determined by evaluating the retention rate, marginal integrity, and postoperative sensitivity at the following three levels: baseline, 6 months, and 18 months by following the Modified USPHS criteria introduced by Vanherle et al. Results: In the present study, the retention rate at 18 month in Group A, Group B, and Group C of 96%, 92%, and 92% was observed, respectively. A marginal integrity at 18 months was 88%, 80%, and 84% for Group A, Group B, and Group C, respectively. Postoperative sensitivity at 18 months was 16%, 12%, and 12% for Group A, Group B, and Group C, respectively. Conclusion: The clinical performance of total-etch and self-etch adhesive systems in NCCLs did not differ significantly with regard to the evaluated parameters – retention, marginal integrity, and postoperative sensitivity.
Forest Concessions and the United Nations Sustainable Development Goals: Potentials, Challenges and Ways Forward

The Agenda 2030 and its Sustainable Development Goals (SDGs) have directed increased political attention to forests and their sustainable management globally. Forest concessions are a predominant instrument for the sustainable management of public production natural forests in the tropics, but the relationship between the SDGs and forest concessions is poorly explored. Knowledge of this relationship could facilitate aligning tropical forest concession regimes with the SDGs. This research was conducted by means of an online survey, expert interviews and four regional stakeholder workshops to examine (i) how forest concessions can support the implementation of the SDGs; and (ii) what are the key barriers hindering the potential contributions of forest concessions to the SDG. The findings revealed three broad pathways through which forest concessions can support the implementation of the SDGs: (i) sustainable use and management of ecosystem goods and services as the core business; (ii) provision of public goods for socioeconomic development; and (iii) contribution to (sub) national economies through income, employment and fiscal obligations. The paper identifies region-specific (Africa, Latin America and Southeast Asia) technical, legal, governance and institutional barriers limiting the potential contributions. Among these, the key barriers are unclear and conflicting tenure, and the lack of available technical and qualified personnel. The paper concludes that the contributions of forest concessions to the SDGs depend on governance context and the clear use of the instrument to deliver such objectives as better planned and implemented concessions and binding concession contracts. The paper also provides recommendations for aligning forest concessions with the SDGs.
Familial tendency for hypertension is associated with increased vascular stiffness.

OBJECTIVE Hypertension is the leading cause of cardiovascular disease and premature death. New methods for early detection of hypertension and its consequences can reduce complications arising from uncontrolled hypertension. Pulse-wave velocity (PWV), a measure of arterial stiffness, has been recognized as a valuable tool in assessing risk for cardiovascular complications, although its use in clinical practice is currently limited. Here we examine whether brachial--ankle PWV (baPWV) and femoral--ankle PWV (faPWV) are elevated in nonhypertensive volunteers, with and without a history of familial hypertension. METHODS Volunteers were recruited and questioned as to their medical background and family history. Participants were divided into two groups based on history of familial hypertension and were measured for baPWV and faPWV. Carotid--femoral PWV was computed from these measurements. RESULTS A total of 82 healthy nonhypertensive volunteers (mean age 31.4 ± 9.6) were recruited. Among the study cohort, 43.7% had a history of familial hypertension. There were no between-group differences in any other clinical or demographic characteristics. Both baPWV and faPWV were significantly elevated in volunteers with a history of familial hypertension (10.86 ± 1.69 vs. 9.68 ± 1.52 m/s, P < 0.004, and 7.01 ± 1.65 vs. 6.28 ± 1.26 m/s, P < 0.028, respectively). CONCLUSION Volunteers with a history of familial hypertension present with elevated baPWV and faPWV. This is suggestive of increased central and peripheral arterial stiffness in susceptible individuals before the onset of hypertension. Routine measurement of these parameters may allow for early intervention and risk stratification, especially in persons with a history of familial hypertension.
A primer on single-cell genomics in myeloid biology

Purpose of review Understanding the fast-moving field of single-cell technologies, as applied to myeloid biology, requires an appreciation of basic molecular, informatics, and biological concepts. Here, we highlight both key and recent articles to illustrate basic concepts for those new to molecular single-cell analyses in myeloid hematology. Recent findings Recent studies apply single-cell omics to discover novel cell populations, construct relationships between cell populations, reconfigure the organization of hematopoiesis, and study hematopoietic lineage tree and fate choices. Accompanying development of technologies, new informatic tools have emerged, providing exciting new insights. Summary Hematopoietic stem and progenitor cells are regulated by complex intrinsic and extrinsic factors to produce blood cell types. In this review, we discuss recent advances in single-cell omics to profile these cells, methods to infer cell type identify, and trajectories from molecular omics data to ultimately derive new insights into hematopoietic stem and progenitor cell biology. We further discuss future applications of these technologies to understand hematopoietic cell interactions, function, and development. The goal is to offer a comprehensive overview of current single-cell technologies and their impact on our understanding of myeloid cell development for those new to single-cell analyses.
Coenzyme Q10 Efficacy Test for Human Skin Equivalents Using a Pumpless Skin-On-A-Chip System

A human skin equivalent (HSE) composed of the epidermis and dermis is cultured using a pumpless skin-on-a-chip system to supply cultures the desired flow rate using gravity flow without a pump or an external tube connection. Coenzyme Q10 efficacy is tested by adjusting its concentration, as it is known to have anti-aging and antioxidant effects in culture solutions. The relationship between the contraction rate of a full-thickness human skin equivalent and secreted transforming growth factor (TGF) β-1 is analyzed via enzyme-linked immunosorbent assay (ELISA). Following hematoxylin and eosin (H&E) staining, an image of the skin equivalent is analyzed to measure the epidermal layer’s thickness. The cell density and differentiation of the dermis layer are investigated. Gene and protein expression in the dermal and epidermal layers are quantitatively analyzed using quantitative real time polymerase chain reaction (qPCR) and immunohistochemical staining. As the coenzyme Q10 treatment concentration increased, the number of cells per unit area and the thickness of the epidermal layer increased, the expression level of filaggrin increased, and the contraction rate of full-thickness HSE was proportional to the amount of TGF β-1 secreted.
Summarizing Online Conversations: A Machine Learning Approach

Summarization has emerged as an increasingly useful approach to tackle the problem of information overload. Ex-tracting information from online conversations can be of very good commercial and educational value. But majority of this information is present as noisy unstructured text making traditional document summarization techniques dif-(cid:12)cult to apply. In this paper, we propose a novel approach to address the problem of conversation summarization. We develop an automatic text summarizer which extracts sentences from the conversation to form a summary. Our approach consists of three phases. In the (cid:12)rst phase, we prepare the dataset for usage by correcting spellings and segmenting the text. In the second phase, we represent each sentence by a set of prede(cid:12)ned features. These features capture the statistical, linguistic and sentimental aspects along with the dialogue structure of the conversation. Finally, in the third phase we use a machine learning algorithm to train the summarizer on the set of feature vectors. Experiments performed on conversations taken from the technical domain show that our system signi(cid:12)cantly outperforms the baselines on ROUGE F-scores.
Etude du transfert de l'ozone dans les colonnes à bulles : Modélisation, applications au traitement des eaux potables et à la désodorisation de gaz soufrés

Notre travail apporte une contribution a la mise en uvre des techniques d'ozonation actuellement tres employees pour le traitement des fluides. L'ecoulement des phases et le transfert de l'ozone dans une colonne a bulles sont etudies a partir d'experiences effectuees sur une unite pilote fonctionnant a contre-courant. Une modelisation des phenomenes est egalement proposee. La determination de la distribution des temps de sejour en fonction des debits conduit a compartimenter la colonne en trois zones d'agitation variable. La mesure des profils d'ozone dissous et de l'efficacite de transfert confirme ce resultat et permet d'etablir une relation donnant k#la en fonction de la vitesse superficielle du gaz et du regime d'ecoulement du liquide. Plusieurs logiciels sont elabores afin de modeliser les profils d'ozone dans l'air et dans l'eau en fonction du type d'ecoulement et des caracteristiques de l'eau a traiter. Seul le modele base sur trois zones d'agitation variable donne des profils en accord avec les mesures experimentales. Un procede de traitement d'atmosphere chargee en composes soufres est egalement etudie. Il met en uvre l'absorption des polluants par une solution aqueuse basique sur une tour garnie, et sa regeneration en continu par oxydation a l'ozone des composes absorbes, sur une colonne a bulles. De bons rendements d'elimination sont obtenus. Les parametres influant le procede sont determines et optimises en vue du dimensionnement d'une installation industrielle. La consommation de solution de lavage est faible, les effluents produits sont bien oxydes et donc traitables dans une filiere d'epuration classique. La faible dose d'ozone necessaire a l'oxydation des polluants rend le procede interessant au niveau economique
Food preference of insects in capoeira area in east of state of Maranhão,
 Brazil

In the relation between plants and herbivores, both organisms developed along their evolutionary histories mediating mechanisms for these relationships. Considering that the present work had the objective to evaluate the herbivory food preference of plants by insects in an area of capoeira phytophysiognomy and the hypothesis tested was that insects prefer plants with lower leaf tenacity. The study was carried out in Caxias, Maranhao, Brazil, in November 2016, in which 600 leaves were analyzed with half of tenacious leaves and one of soft leaves. The chi-square test indicated an association between the variables tested pointing an occurrence of herbivory preference for plants with tenacious leaves.
Modern Organic Synthesis: An Introduction

Synthetic Design - Stereochemical Considerations in Planning Synthesis - The Concept of Protecting Functional Groups - Functional Group Transformations I: Oxidation and Reduction - Functional Group Transformations II: Reactions of Carbon-Carbon p-Bonds - Formations of C-C Single Bonds via Encolate Anions - Formation of C-C Bonds via Organomettalics - Formation of Carbon-Carbon p-Bonds - Synthesis of Carbocyclic Systems