Miracle-dz/UniFAR-FTUs huggingface dataset:
Automated Manufacture of Autologous CD19 CAR-T Cells for Treatment of Non-hodgkin Lymphoma

Chimeric antigen receptor T cells (CAR-T cell) targeting CD19 are effective against several subtypes of CD19-expressing hematologic malignancies. Centralized manufacturing has allowed rapid expansion of this cellular therapy, but it may be associated with treatment delays due to the required logistics. We hypothesized that point of care manufacturing of CAR-T cells on the automated CliniMACS Prodigy® device allows reproducible and fast delivery of cells for the treatment of patients with non-Hodgkin lymphoma. Here we describe cell manufacturing results and characterize the phenotype and effector function of CAR-T cells used in a phase I/II study. We utilized a lentiviral vector delivering a second-generation CD19 CAR construct with 4-1BB costimulatory domain and TNFRSF19 transmembrane domain. Our data highlight the successful generation of CAR-T cells at numbers sufficient for all patients treated, a shortened duration of production from 12 to 8 days followed by fresh infusion into patients, and the detection of CAR-T cells in patient circulation up to 1-year post-infusion.
Simple Flow Tanks for Research and Teaching

Whether from fashion or force of habit, few biologists devote much attention to the characteristics and consequences of fluids in motion. Few laboratories consider a flow tank an essential item in their experimental armamentarium. Yet most organisms both live in and contain moving fluids, and many of their features reflect adaptation to local fluid motion. The fluids of common concern are, of course, air and water; they are moved for experimental purposes with wind tunnels and flow tanks. Present attention will focus on the latter, because wind tunnels have been discussed elsewhere (Vogel 1969). Our general points are, first, that flow tanks may be constructed without great expenditure of time or money and, second, that they are exceedingly useful in a wide variety of investigations, demonstrations, and student projects in several different areas of biology. In particular, we wish to suggest a specific, highly flexible design for flow tanks.
Mindfulness Meditation Biases Visual Temporal Order Discrimination but Not Under Conditions of Temporal Ventriloquism

This study examined how cognitive plasticity acquired from a long (8 weeks) course of mindfulness training can modulate the perceptual processing of temporal order judgment (TOJ) on a sub-second scale. Observers carried out a TOJ on two visual disks, with or without concurrent paired beeps. A temporal ventriloquism paradigm was used in which the sound beeps either were synchronized with the two disks or bracketed the visual stimuli by leading the first disk by 50 ms and lagging the other by 50 ms. A left-to-right bias in TOJ was found under the visual-only condition after mindfulness training. This bias was positively correlated with “acting with awareness,” a factor in the Five Facet Mindfulness Questionnaire, showing that awareness of every moment and enhanced attention focus magnify the left-to-right bias. However, the effect of mindfulness training may be short-lived and was not present when attention was diverted by auditory events in the cross-modal temporal ventriloquism illusion.
SLC20A1 Is Involved in Urinary Tract and Urorectal Development

Previous studies in developing Xenopus and zebrafish reported that the phosphate transporter slc20a1a is expressed in pronephric kidneys. The recent identification of SLC20A1 as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role of SLC20A1 in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish ortholog slc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed immunohistochemistry of an unaffected 6-week-old human embryo and detected SLC20A1 in the urinary tract and the abdominal midline, structures implicated in the pathogenesis of cloacal exstrophy. Additionally, we resequenced SLC20A1 in 690 individuals with bladder exstrophy-epispadias complex (BEEC) including 84 individuals with cloacal exstrophy. We identified two additional monoallelic de novo variants. One was identified in a case-parent trio with classic bladder exstrophy, and one additional novel de novo variant was detected in an affected mother who transmitted this variant to her affected son. To study the potential cellular impact of SLC20A1 variants, we expressed them in HEK293 cells. Here, phosphate transport was not compromised, suggesting that it is not a disease mechanism. However, there was a tendency for lower levels of cleaved caspase-3, perhaps implicating apoptosis pathways in the disease. Our results suggest SLC20A1 is involved in urinary tract and urorectal development and implicate SLC20A1 as a disease-gene for BEEC.
Older Adults' Physical Activity and the Relevance of Distances to Neighborhood Destinations and Barriers to Outdoor Mobility

Aim: To determine the relevance of features located close to home and further away, our aim was to study associations between older adults' physical activity and self-reported neighborhood destinations and barriers to outdoor mobility categorized by presence and maximal distance from home. Methods: Cross-sectional analyses comprising men and women 79–94 years old (57%) living independently in Central Finland (n = 185). Self-reported physical activity was categorized into lower (≤3 h moderate activity a week) and higher (≥4 h moderate or intense activity a week) activity. Assisted by interviewers, participants located on an interactive map destinations perceived to facilitate and barriers perceived to hinder outdoor mobility in their neighborhood. Participants' home addresses were geolocated. Euclidean distances between home and reported locations were computed, and the maximal distance from home to neighborhood destinations and barriers, respectively, was categorized based using four common buffer distances, i.e., 250 m, 500 m, 750 m, and 1 km. Participants reporting destinations or barriers within and beyond the respective distance were compared with those reporting none. Results: About 80% of participants reported neighborhood destinations and 55% neighborhood barriers to outdoor mobility. Barriers were generally located closer to home than destinations [median 166 m (range 25 m−6.10 km) vs. 492 m (5 m−2.7 km)]. Logistic regression analyses adjusted for age, sex, and physical performance showed that neighborhood destinations increased the odds for higher physical activity when located beyond 500 m from home [OR 2.95, 95% confidence interval (CI) 1.02–8.54], but not when located solely within 500 m (OR 1.70, 95% CI 0.30–9.61), in comparison with when reporting no destinations. In contrast, neighborhood barriers decreased the odds for higher physical activity when solely located within 500 m (OR 0.31, 95% CI 0.14–0.72), but not when any barrier was located beyond 500 m (OR 0.96, 95% CI 0.23–3.99), compared with when reporting no barriers. Associations were similar for 250-m buffer distances, but not robust for 750-m and 1,000-m buffers because of lower prevalence. Conclusion: Neighborhood barriers to outdoor mobility located close to home were associated with lower physical activity of older adults, whereas barriers further away were not. Attractive destinations for outdoor mobility located further away from home correlated with higher physical activity, potentially by motivating one to go out and be physically active. Temporal relationships warrant further study.
A method for assessing the field susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica)

SUMMARY In experiments to develop a method for assessing the field susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica) seed tubers were stab-inoculated near the stolon (attachment end), with a suspension of the bacterium, or with water, before planting. Disease symptoms were recorded in three years (1980–1982) and plant growth and yield in 1982. Estima and Maris Bard were the most susceptible cultivars with many plants failing to emerge and most of those that did showing disease symptoms. Pentland Crown was the most resistant: few plants failed to emerge and few showed blackleg. Nevertheless compared with water-inoculated plants bacterial inoculation of the seed tubers of this cultivar caused loss of yield and differences in tuber size distribution. Cara, Wilja and King Edward showed intermediate reactions.
Childhood Maltreatment Alters the Neural Processing of Chemosensory Stress Signals

Accumulating evidence suggests that childhood maltreatment (CM) confers risk for psychopathology later in life by inducing hypervigilance to social threat cues such as fearful faces. However, it remains unclear whether the modulatory impact of CM extents to the olfactory domain of social communication in humans. To address this question, we examined whether CM modulates the neural processing of chemosensory threat signals in sweat and whether CM affects the stress-reducing effects of oxytocin (OXT) in this context. In a randomized, double-blind within-subject functional MRI study design, 58 healthy participants (30 females) received intranasal OXT (40 IU) or placebo (PLC) and completed a forced-choice emotion recognition task with faces of varying emotion intensities (neutral to fearful) while exposed to sweat stimuli and a non-social control odor. Axillary sweat samples were collected from 30 healthy male donors undergoing an acute psychosocial stressor (stress) and ergometer training (sport) as control in a pre-study. CM was assessed by the 25-item Childhood Trauma Questionnaire (CTQ). The final fMRI analysis included 50 healthy participants (26 females). Regression analysis showed a stress-specific association of CTQ scores with amygdala hyperreactivity, hippocampal deactivation, and increased functional connectivity between the amygdala and the hippocampus, medial orbitofrontal cortex, and the anterior cingulate cortex (ACC) under PLC. Furthermore, we observed a positive association of CTQ scores and the dampening effects of OXT on stress-related amygdala responses. Our findings suggest that CM may induce hypervigilance to chemosensory threat cues in a healthy sample due to inefficient frontolimbic inhibition of amygdala activation. Future studies should investigate whether increased recruitment of the intralimbic amygdala-hippocampus complex reflects a compensatory mechanism that prevents the development of psychopathology in those who have experienced CM. Furthermore, the results reveal that the stress-specific effects of OXT in the olfactory domain are more pronounced in participants with increasing levels of CM exposure.
High biodiversity in Polychelida crustaceans from the Jurassic La Voulte-sur-Rhône Lagerstätte

ABSTRACT The Middle Jurassic La Voulte-sur-Rhône Lagerstätte preserves with remarkable details a highly diversified bathyal palaeocommunity dominated by arthropods. Polychelida Scholtz & Richter, 1995 are particularly abundant and are currently represented by Eryon ellipticus Van Straelen, 1923, Proeryon giganteus (Van Straelen, 1923), Hellerocaris falloti (Van Straelen, 1923), and Willemoesiocaris ovalis (Van Straelen, 1923). This new investigation reveals the presence of two new genera and three new species in this crustacean community: Voulteryon parvulus n. gen., n. sp. , Cycleryon romani n. sp. , which is the oldest occurrence of the genus, and Adamanteryon fourneti n. gen., n. sp. , an enigmatic polychelidan of uncertain affinities. The genus Proeryon Beurlen, 1928 is also revised to solve problems of homonymy, and as a consequence we here propose the replacement name Proeryon zehentbaueri pro Proeryon giganteus Beurlen, 1930, non Proeryon giganteus (Van Straelen, 1923). These new results place the La Voulte polychelidan lobster community at the first rank in terms of familial and generic diversity (three families, seven genera) and at the second rank in terms of species diversity (seven species) just after the Late Jurassic Eichstätt Lagerstätte (eight species). Thanks to modern techniques, such as X-ray tomography, this study also documents structures never observed before such as thoracic appendages of Proeryon giganteus and Hellerocaris falloti. In the case of Hellerocaris falloti, these new observations suggest it is one of the closest fossil relatives to extant polychelids.
Identification of a Metagenome-Assembled Genome of an Uncultured Methyloceanibacter sp. Strain Acquired from an Activated Sludge System Used for Landfill Leachate Treatment

Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used for landfill leachate treatment at a closed landfill site. The total size and encoded sequences are 3,407,434 bp and 3,280 genes, respectively. ABSTRACT Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used for landfill leachate treatment at a closed landfill site. The total size and encoded sequences are 3,407,434 bp and 3,280 genes, respectively.
Hepatoprotective efficacy of Hypnea muciformis ethanolic extract on CCl4 induced toxicity in rats

The ethanolic extract of Hypnea muciformis (red algae) was tested for hepatoprotective activity against experimentally induced liver damage by Carbon tetrachloride (CCl4) in male albino rats. The levels of serum enzymatic and biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), lactate dehydrogenase, 5' nucleotidase, bilirubin, creatinine, urea, triglycerides, lipid peroxides and albumin were determined. The CCI4 induced lesions in the liver significant increased the levels of serum marker enzymes SGPT and SGOT, bilirubin, creatinine and decreased urea. The oral treatment with ethanolic extract of H. muciformis exhibited significant hepatoprotective activity by reducing the CCL4 caused changes in the biochemical parameters such as total protein, total bilirubin, total cholesterol, triglycerides, and urea. These parameters were restored towards the normal levels as shown by the enzymatic tests. In addition, H. muciformis significantly decreased the liver weight of CCl4 intoxicated rats. Apparently the H. muciformis extract interfered with the free radical formation, which resulted in hepatoprotective activity. Acute toxicity studies revealed that the LD50 value was more than 3 g/kg body weight. These results clearly indicated that this seaweed contained some active principles in its ethanolic extract which acted as an antidote against the hepatotoxicity induced by CCl4.
eXtream: a System for Real-time Monitoring of Dynamic Web Sources

In this work, we introduce eXtream, a Big Data platform whose main goal is to deploy modular and customisable processing topologies for massive analysis of web data in real time. The system offers a reduced group of pre-installed modules that can be easily combined in a visual way. Additionally, an advanced user can upload new modules and extend an existing topology. This tool facilitates the development of many Information Retrieval and Big Data applications, such as query-based real-time filtering or topic analysis services on Social Media data. To demonstrate it, we have also developed an initial web-based demonstrator.
Close coupling between phytoplankton growth and microzooplankton grazing in the western South China Sea

We conducted 28 dilution experiments during August‐September 2007 to investigate the coupling of growth and microzooplankton grazing rates among ultraphytoplankton populations and the phytoplankton community and their responses to habitat variability (open‐ocean oligotrophy, eddy‐induced upwelling, and the Mekong River plume) in the western South China Sea. At the community level, standing stocks, growth, and grazing rates were strongly and positively correlated, and were related to the higher abundance of larger phytoplankton cells (diatoms) at stations with elevated chlorophyll concentration. Phytoplankton growth rates were highest (>2 d−1) within an eastward offshore jet at 13°N and at a station influenced by the river plume. Among ultraphytoplankton populations, Prochlorococcus dominated the more oceanic and oligotrophic stations characterized by generally lower biomass and phytoplankton community growth, whereas Synechococcus became more important in mesotrophic areas (eddies, offshore jet, and river plume). The shift to Synechococcus dominance reflected, in part, its higher growth rates (0.87 ± 0.45 d−1) compared to Prochlorococcus (0.65 ± 0.29 d−1) or picophytoeukaryotes (0.54 ± 0.50 d−1). However, close coupling of microbial mortality rates via common predators is seen to play a major role in driving the dominance transition as a replacement of Prochlorococcus, rather than an overprinting of its steady‐state standing stock.
Effect of physiological integration in self/non-self genotype recognition on the clonal invader Carpobrotus edulis

Aims Biological invasions represent one of the most important threats to the conservation of biodiversity; however, the mechanisms underlying successful invaders remain unsolved. Many of the most aggressive invaders show clonal growth, and capacity for clonal integration has been pointed out recently as an important trait explaining the success of invasive plants. We aim to determine the role of physiological integration in the capacity for self/non-self genotype recognition in the clonal invader Carpobrotus edulis and the implications of this capacity for the expansion of this aggressive invader.
Locational (In-)Efficiency of Renewable Power Generation Feeding in the Electricity Grid: A Spatial Regression Analysis

This paper analyzes the negative external effects caused by the introduction of variable renewable energy sources into an inflexible power system. We investigate the costs that arise due to the need for temporarily reducing their output to relief grid overstress in the case of high electricity feed-in. The responsible system operator has to remunerate the power plant operator for this lost output. The resulting costs for the system operator, the so-called feed-in management costs, are passed on to the end-consumers in the respective region via the grid use tariff scheme. In this paper, we develop a two-part regression model that explains (i) the occurrence of feed-in management and (ii) the regional variation of feed-in management costs. In the first part, we use a logit model to explain why some regions experienced feed-in management in recent years and others did not. The second part covers an augmented spatial econometric model that investigates the interregional variability of feed-in management costs. The estimates of both models show that especially the installed capacity of wind energy connected to the medium and high voltage level have a negative impact on feed-in management and that high load in a region reduces the need for feed-in management measures. The augmented spatial model indicates for the case of four major DSOs in Germany that an increase by 1 MW of wind energy capacity at the medium and high voltage level lead to an increase in feed-in management costs by 1.9% and 0.9% in regions that already experienced feed-in management, respectively. The policy implication of this finding is that price signals for the reinforcement of the grid infrastructure or for the siting of VRES should be given.
On the design of quasi-orthogonal constellations: filling the empty threads

A systematic approach for constructing full diversity quasi-orthogonal constellation designs with rate 1 symbol per channel use is studied. The approach complements orthogonal constellation matrices to have rate one symbol per channel use while preserving the full diversity property of these matrices. A design example based on 4 transmit antennas, illustrates filling the empty thread in the orthogonal constellation matrix of rate 3/4 in order to obtain a rate one full diversity quasi-orthogonal constellation. The proposed approach is general for all orthogonal constellations having a threaded architecture, which includes most of the orthogonal constellations documented in the literature.
Knowledge Based Transformer Model for Information Retrieval

Vocabulary mismatch is a frequent problem in information retrieval (IR). It can occur when the query is short and/or ambiguous but also in specialized domains where queries are made by non-specialists and documents are written by experts. Recently, vocabulary mismatch has been addressed with neural learning-to-rank (NLTR) models and word embeddings to avoid relying only on the exact matching of terms for retrieval. Another approach to vocabulary mismatch is to use knowledge bases (KB) that can associate different terms to the same concept. Given the recent success of transformer encoders for NLP, we propose KTRel: a NLTR model that uses word embeddings, Knowledge bases and Transformer encoders for IR.
A rapid detection of organophosphorus resistance with insensitive acetylcholinesterase in spider mites Tetranychus urticae Koch on cotton

Resistance management strategy requires simple detection methods by which resistant pests can be detected in the field and in early stages of resistance development. Because of the insensitivity of acetylcholinesterase is the most common cause of organophosphorus resistance in spider mites T. urticae, the activity of acetylcholinesterase and its inhibition with paraoxon were measured in spider mites susceptible strain S, resistant strain R and in two field strains collected from cotton fields in Kafr El‐Sheikh province KR and in Beheira province BR in Egypt. The resistant strain was 102‐fold resistant to parathion, while the two field strains KR and BR were 36‐fold and 17‐fold resistant to parathion, respectively. The activity of acetylcholinesterase in R strain and in the two field strains KR and BR are significantly lower than in the S strain, where the percent of activity in relation to that of S strain were 60%, 72% and 75% for R, KR and BR strains, respectively. The reduction in rate of acetylcholinesterase inhibition was 30 times, 27.5 times and 13 times in R, KR and BR strains for paraoxon, respectively. The broken curves for the two field strains showed heterogeneity in the spider mites population, where the resistant portion was 82% and 48% for KR strain and BR strain. This quick and simple technique can be directly performed for the field strains and provides us with reproducible data about the development of resistance in the field.
ntroduction

Although male heads of state wear suits at summit meetings, male job appli-cants wear them to interviews, and men accused of rape and murder wear them in court. the pants-jacket-shirt-and-tie costume, formal or informal, is often called boring or worse. but men’s suits are neither post-modern nor minimalist, multicultural nor confessional – they are relentlessly modern, in the best classical sense.
Isolation and characterization of ten microsatellite loci for Senegal sole (Solea senegalensis Kaup)

Senegal sole ( Solea senegalensis Kaup) is a high-value marine flatfish exploited in both fisheries and aquaculture. Here we describe the isolation of seven tetranucleotide and three dinucleotide polymorphic microsatellite loci. Characteristics of a captive broodstock and a wild population are described. Three loci displayed a significant departure from Hardy- Weinberg expectations in both populations, suggesting a substantial frequency of null alleles. The remaining seven loci were found to be in equilibrium in the wild population, whereas only four of them were in the cultured population. Cross-species amplification was successful for three loci in other five flatfishes.