Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessing Histology Structures by Ex Vivo MR Microscopy and Exploring the Link Between MRM-Derived Radiomic Features and Histopathology in Ovarian Cancer

The value of MR radiomic features at a microscopic scale has not been explored in ovarian cancer. The objective of this study was to probe the associations of MR microscopy (MRM) images and MRM-derived radiomic maps with histopathology in high-grade serous ovarian cancer (HGSOC). Nine peritoneal implants from 9 patients with HGSOC were imaged ex vivo with MRM using a 9.4-T MR scanner. All MRM images and computed pixel-wise radiomics maps were correlated with the slice-matched stroma and tumor proportion maps derived from whole histopathologic slide images (WHSI) of corresponding peritoneal implants. Automated MRM-derived segmentation maps of tumor and stroma were constructed using holdout test data and validated against the histopathologic gold standard. Excellent correlation between MRM images and WHSI was observed (Dice index = 0.77). Entropy, correlation, difference entropy, and sum entropy radiomic features were positively associated with high stromal proportion (r = 0.97,0.88, 0.81, and 0.96 respectively, p < 0.05). MR signal intensity, energy, homogeneity, auto correlation, difference variance, and sum average were negatively associated with low stromal proportion (r = –0.91, –0.93, –0.94, –0.9, –0.89, –0.89, respectively, p < 0.05). Using the automated model, MRM predicted stromal proportion with an accuracy ranging from 61.4% to 71.9%. In this hypothesis-generating study, we showed that it is feasible to resolve histologic structures in HGSOC using ex vivo MRM at 9.4 T and radiomics.
L-Type Calcium Channels Contribute to Ethanol-Induced Aberrant Tangential Migration of Primordial Cortical GABAergic Interneurons in the Embryonic Medial Prefrontal Cortex

Abstract Exposure of the fetus to alcohol (ethanol) via maternal consumption during pregnancy can result in fetal alcohol spectrum disorders (FASD), hallmarked by long-term physical, behavioral, and intellectual abnormalities. In our preclinical mouse model of FASD, prenatal ethanol exposure disrupts tangential migration of corticopetal GABAergic interneurons (GINs) in the embryonic medial prefrontal cortex (mPFC). We postulated that ethanol perturbed the normal pattern of tangential migration via enhancing GABAA receptor-mediated membrane depolarization that prevails during embryonic development in GABAergic cortical interneurons. However, beyond this, our understanding of the underlying mechanisms is incomplete. Here, we tested the hypothesis that the ethanol-enhanced depolarization triggers downstream an increase in high-voltage-activated nifedipine-sensitive L-type calcium channel (LTCC) activity and provide evidence implicating calcium dynamics in the signaling scheme underlying the migration of embryonic GINs and its aberrance. Tangentially migrating Nkx2.1+ GINs expressed immunoreactivity to Cav1.2, the canonical neuronal isoform of the L-type calcium channel. Prenatal ethanol exposure did not alter its protein expression profile in the embryonic mPFC. However, exposing ethanol concomitantly with the LTCC blocker nifedipine prevented the ethanol-induced aberrant migration both in vitro and in vivo. In addition, whole-cell patch clamp recording of LTCCs in GINs migrating in embryonic mPFC slices revealed that acutely applied ethanol potentiated LTCC activity in migrating GINs. Based on evidence reported in the present study, we conclude that calcium is an important intracellular intermediary downstream of GABAA receptor-mediated depolarization in the mechanistic scheme of an ethanol-induced aberrant tangential migration of embryonic GABAergic cortical interneurons.
A power system fault diagnosis method based on DS evidence theory

A power system fault diagnosis method based on DS evidence theory is proposed, which can deal with uncertainty brought by unknown. After constructing frame of discernment, DS evidences are expressed based on Bayesian method, and a combined belief function is obtained in terms of Dempster Rule, in accordance with which, the fault elements are recognized. The algorithm is realized by programming based on Matlab, and three typical fault conditions which are single fault with protection and circuit breaker working normally, single fault with misoperation of protection and refuse of circuit breaker, multiple faults with misoperation and refuse of protection and circuit breaker are analyzed. The testing results demonstrate that this method can recognize the fault elements efficiently.
Nandrolone supplementation does not improve functional recovery in an aged animal model of volumetric muscle loss injury

Aging hinders the effectiveness of regenerative medicine strategies targeting the repair of volumetric muscle loss (VML) injury. Anabolic steroids have been shown to improve several factors which contribute to the age‐related decline in muscle's regenerative capacity. In this study, the impact of exogenous nandrolone decanoate (ND) administration on the effectiveness of a VML regenerative repair strategy was explored using an aged animal model. Unilateral tibialis anterior VML injuries were repaired in 18‐month‐aged animal models (male Fischer 344 rat) using decellularized human skeletal muscle scaffolds supplemented with autologous minced muscle. The contralateral limb was left untreated/uninjured. Following repair, ND(+) or a carrier control (ND−) was delivered via weekly injection for a period of 8 weeks. At 8 weeks, muscle isometric torque, gene expression, and tissue structure were assessed. ND(+) treatment did not improve contractile torque recovery following VML repair when compared to carrier only ND(−) injection controls. Peak isometric torque in the ND(+) VML repair group remained significantly below contralateral uninjured control values (4.69 ± 1.18vs. 7.46 ± 1.53 N mm/kg) and was statistically indistinguishable from carrier only ND(−) VML repair controls (4.47 ± 1.18 N mm/kg). Gene expression for key myogenic genes (Pax7, MyoD, MyoG, IGF‐1) were not significantly elevated in response to ND injection, suggesting continued age related myogenic impairment even in the presence of ND(+) treatment. ND injection did reduce the histological appearance of fibrosis at the site of VML repair, and increased expression of the collagen III gene, suggesting some positive effects on repair site matrix regulation. Overall, the results presented in this study suggest that a decline in regenerative capacity with aging may present an obstacle to regenerative medicine strategies targeting VML injury and that the delivery of anabolic stimuli via ND administration was unable to overcome this decline.
Prdm6 controls heart development by regulating neural crest cell differentiation and migration

The molecular mechanisms that drive the acquisition of distinct neural crest cell (NCC) fates is still poorly understood. Here, we identified Prdm6 as an epigenetic modifier that temporally and spatially regulates the expression of NCC specifiers and determines the fate of a subset of migrating cardiac NCCs (CNCCs). Using transcriptomic analysis and genetic and fate mapping approaches in transgenic mice, we showed that disruption of Prdm6 was associated with impaired CNCC differentiation, delamination, and migration and led to patent ductus arteriosus (DA) and ventricular noncompaction. Bulk and single-cell RNA-Seq analyses of the DA and CNCCs identified Prdm6 as a regulator of a network of CNCC specification genes, including Wnt1, Tfap2b, and Sox9. Loss of Prdm6 in CNCCs diminished its expression in the pre-epithelial–mesenchymal transition (pre-EMT) cluster, resulting in the retention of NCCs in the dorsal neural tube. This defect was associated with diminished H4K20 monomethylation and G1-S progression and augmented Wnt1 transcript levels in pre-EMT and neural tube clusters, which we showed was the major driver of the impaired CNCC migration. Altogether, these findings revealed Prdm6 as a key regulator of CNCC differentiation and migration and identified Prdm6 and its regulated network as potential targets for the treatment of congenital heart diseases.
Transgender Parents: Negotiating “Coming Out” and Gender Affirmation with Children and Co-Parents

ABSTRACT This study investigated how transgender parents negotiate “coming out” and pursuit of gender affirmation (GA) with their children and co-parents. Sixty-six open-ended survey responses and 38 one-on-one interviews conducted with Australian trans parents, aged 24–67 years, were analyzed using thematic analysis. The main themes were: (i) Anticipating their Response: “Coming Out” to co-parents and children; (ii) “Having that talk”—Negotiating disclosure of trans identity with co-parents and children; and (iii) Negotiating Gender Affirmation alongside Parenting. “Coming out” was experienced as both vital and a point of vulnerability, with GA necessitating communication and negotiation alongside parenting. Many participants reported significant anxiety before “coming out” to co-parents and children. Children’s age was an influential factor in reaction to changes in parental gender identity, with younger and adult children reportedly being the most receptive. Results are discussed in terms of the reported benefits of pursuing GA for trans parents.
Liquid-amplified zipping actuators for micro-air vehicles with transmission-free flapping

Flapping micro-air vehicles (MAVs) can access a wide range of locations, including confined spaces such as the inside of industrial plants and collapsed buildings, and offer high maneuverability and tolerance to disturbances. However, current flapping MAVs require transmission systems between their actuators and wings, which introduce energetic losses and additional mass, hindering performance. Here, we introduce a high-performance electrostatic flapping actuation system, the liquid-amplified zipping actuator (LAZA), which induces wing movement by direct application of liquid-amplified electrostatic forces at the wing root, eliminating the requirement of any transmission system and their associated downsides. The LAZA allows for accurate control of flapping frequency and amplitude, exhibits no variation in performance over more than 1 million actuation cycles, and delivers peak and average specific powers of 200 and 124 watts per kilogram, respectively, exceeding mammalian and insect flight muscle and on par with modern flapping MAV actuation systems. The inclusion of 50-millimeter-long passively pitching wings in a dragonfly-sized LAZA flapping system allowed the rectification of net directional thrust up to 5.73 millinewtons. This thrust was achieved while consuming only 243 milliwatts of electrical power, implying a thrust-to-power ratio of 23.6 newtons per kilowatt, similar to state-of-the-art flapping MAVs, helicopter rotors, and commercial drone motors. Last, a horizontally moving LAZA flapping system supported by a taut nylon wire was able to accelerate from at-rest and travel at speeds up to 0.71 meters per second. The LAZA enables lightweight, high-performance transmission-free flapping MAVs for long-term remote exploration and search-and-rescue missions. Description Liquid-amplified zipping enables transmission-free flapping for high-performance lightweight micro-air vehicles.
Psychological first aid training in disaster preparedness for nurses working with emergencies and traumas.

AIM This study aimed at evaluating the effects of a modified psychological first aid (PFA) training program on nurses' psychological preparedness for emergencies and disasters. BACKGROUND Nurses need to be psychologically prepared in order to mitigate the impact of conflicts and disasters. The PFA training could ensure their psychological preparedness in responding to different emergencies and disasters. DESIGN A nonequivalent controlled group trial was conducted between July 2019 and August 2019. METHODS Nurses working in the trauma units from five hospitals in Palestine were allocated into two arms: a control arm and an intervention arm incorporating a 9-hour modified PFA training program. Both groups were assessed at start (pretest) and upon completion of the program (post test) using a number of validated self-reports. RESULTS Eighty-four nurses were allocated to each arm, and 75 in each arm completed baseline data (n = 150). The results of generalized estimating equation estimates revealed that PFA training imposed a significant group-by-time effect on psychological preparedness (p = 0.013). The mean scores of psychological preparedness showed greater improvement in the intervention group than in the control group at posttest (37.81 vs. 32.64). Improved group-by-time effect was further identified in relation to optimism (p = 0.009), self-esteem (p = 0.008), and self-efficacy (p = 0.033). The intervention effect was large (Cohen d = 1.41). CONCLUSION PFA training may help to build nurses' capacity in being better prepared to respond to psychological issues during and after emergencies and disasters. IMPLICATIONS FOR NURSING AND HEALTH POLICY Nurses should be adequately equipped with knowledge and skills to deal with different emergencies and disasters. Health policy needs to address psychological preparedness for emergencies and disasters of all healthcare workers. Healthcare policymakers and educators could adopt PFA training on a regular basis to consolidate psychological preparedness of nurses.
Three-phase grid-connected inverters with improved DPC-SVM

As dynamic response of the traditional direct power control using space vector modulation (DPC-SVM) is slow, an improved DPC-SVM method for three-phase grid-connected inverters is proposed. The proposed control method is deduced based on the decoupled control idea of the traditional space vector pulse width modulation (SVPWM). The reference power feedforward is used to improve the dynamic response. The operating principles of the traditional and improved DPC-SVM methods are illustrated. Simulation and experimental results of a 15 kVA three-phase grid-connected inverter with traditional and improved control methods verify the theoretical analysis. Compared with the traditional control method, the grid-connected inverter with the improved control method has fast dynamic response.
Comparing muscle VO2 from near-infrared spectroscopy desaturation rate to pulmonary VO2 during cycling below, at and above the maximal lactate steady state.

Muscle oxygen uptake (V̇O2m) evaluated from changes in the near-infrared spectroscopy oxygen desaturation slope during a 5-s arterial blood flow occlusion has been proposed as an estimation of the actual V̇O2m. However, its correspondence with pulmonary oxygen uptake (V̇O2p) during exercise remains unknown. PURPOSE to investigate the V̇O2m and V̇O2p relationship in females and males in response to prolonged constant-load cycling exercise at different intensities. METHODS Eighteen participants (8 females) visited the laboratory on six occasions: 1) ramp incremental test; 2-3) 30-min constant power output (constant-PO) exercise bout to determine the maximal lactate steady state (MLSS); 4-6) constant-PO exercise bouts to task failure at (i) 15% below MLSS (MLSS-15%); (ii) MLSS; (iii) 15% above MLSS (MLSS+15%). V̇O2m was estimated at baseline, at min 5, 10, 20, 30, and at task failure. V̇O2p was continuously recorded during the constant-PO bouts. RESULTS V̇O2pand V̇O2m significantly increased from min 5 to min 30 in MLSS condition (all p < 0.05) and from min 5 to min 10 in MLSS+15% condition (all p < 0.05). V̇O2pand V̇O2m were correlated (r2 adj range of 0.70-0.98, all p < 0.001) amongst exercise intensities in both females and males. Additionally, both variables were also correlated when expressed as percent (r2 adj range of 0.52-0.77, all p < 0.001). CONCLUSION V̇O2p and V̇O2m responses were similar when exercising below, at, and above the MLSS independently of sex. Most importantly, V̇O2p andV̇O2m were correlated regardless the exercise intensity and sex of the participants.
Automatic control system in regenerative aluminum melting furnace

In order to realize the safety,energy saving,continuously run of regenerative aluminum melting furnace,the modern automatic control system was integrated.Based on the analysis of the process characteristics of regenerative aluminum melting furnace,the relationship between the production process and the automatic control system was established,and the appropriate control system and control strategies were established.through the application of the case,the relevant process control strategies are fully implemented and embodied.The result shows that the automatic control system meets the regenerative melting furnace production process requirements.
Progression of Age-Related Macular Degeneration Among Individuals Homozygous for Risk Alleles on Chromosome 1 (CFH-CFHR5) or Chromosome 10 (ARMS2/HTRA1) or Both

Key Points Question What are the independent and combined associations of the 2 most common genetic risk loci for age-related macular degeneration (AMD)—chromosomes 1 (CFH-CFHR5) and 10 (ARMS2/HTRA1)—with disease progression? Findings This case series study of 502 individuals found that, after adjusting for age and baseline AMD severity, compared with individuals with 2 risk alleles exclusively at CFH-CFHR5, the time to conversion to late-stage disease was shortest among carriers of 2 risk alleles at each of the ARMS2/HTRA1 and CFH-CFHR5 loci, followed by individuals with 2 risk alleles exclusively at ARMS2/HTRA1. Meaning These results suggest that the differential association of the 2 major genetic risk loci with disease progression may be substantial and may warrant consideration in AMD clinical research.
Are the nerves supplying the anterior sacroiliac joint nociceptive?

BACKGROUND Sacroiliac joint (SIJ) pain is often difficult to diagnose. Moreover, while its anatomical characteristics have been well studied, its innervation and the contributions of particular nerves remain controversial, especially in relation to posterior joint innervation. To our knowledge, previous studies have not investigated the presence of nociceptive fibers in the nerves innervating the anterior sacroiliac joint. MATERIALS AND METHODS Eight adult cadaveric sides underwent dissection of the anterior sacroiliac joints. Adjacent anterior rami were examined for branches to the anterior sacroiliac joint. Any branches contributing to the anterior sacroiliac joint were measured and then resected. These samples were fixed in formalin and substance P was identified immunohistologically. RESULTS On all sides, 1-2 small branches (mean diameter of 0.33 mm) arose from the posterior aspect of the L4 anterior ramus (12.5%), the L5 anterior ramus (62.5%), or simultaneously from both the L4 and L5 anterior rami (25%). These branches had a mean length of 13.5 mm. All histological samples contained nerve tissue. All samples of nerve fibers traveling to the anterior SIJ were positive for diffuse substance P reactivity. There were no histological differences between sides or sex. Each of the branches identified as traveling to the SIJ exhibited similar positivity for Substance P. CONCLUSIONS This cadaveric study demonstrates that the anterior SIJ nerve fibers carry pain fibers. This new knowledge has application to patients with SIJ syndrome and to its various treatments including interventional approaches to SIJ pain.
Atrial fibrillation detection using a automated electrocardiographic monitoring in a transient ischaemic attack service

Background The vast majority of the transient ischaemic attacks (TIA) services in UK reported significant delays in the initiation of the routine cardiac monitoring that may result in a significant number of missed atrial fibrillation (AF) paroxysms and increased long-term risk of recurrent stroke. Automated continuous ECG monitoring (ACEM) system has shown promising results in terms of AF detection but it is unclear if ACEM improves AF detection in a rapid outpatient TIA service. Objectives We assessed ACEM in patients with TIA with the aim to significantly reduce the delay to initiate the cardiac monitoring and to enhance the yield of AF detection in these patients. We also aimed to determine the impact of a more rapid initiation of ACEM on the 6-month risk of recurrent stroke/TIA. Methods This is an observational, prospective before (phase 1: 1 July to 31 December 2018) versus after (phase 2: 1 January to 30 June 2019) study of the effect of ACEM, compared with routine initiation of 24h-Holter ECG, in patients with TIA assessed in our service. Results The phase 1 (n=136) and phase 2 (n=105) cohorts did not differ with regards to age, risk factors, duration of cardiac monitoring. The rate of newly detected AF was significantly higher in phase 2 compared with phase 1 (9.52% vs 2.21%, p<0.001). The 6-month risk of recurrent stroke/TIA was significantly lower in phase 2 compared with phase 1 (7.4% vs 1%, p=0.018). Conclusions Early initiation of ACEM improves AF detection after TIA in a rapid TIA service and is associated with a reduced risk of recurrent TIA/stroke.
Phase Ib Trial of the Combination of Imatinib and Binimetinib in Patients with Advanced Gastrointestinal Stromal Tumors

Abstract Purpose: This phase Ib trial was designed to evaluate the safety and early efficacy signal of the combination of imatinib and binimetinib in patients with imatinib-resistant advanced gastrointestinal stromal tumors (GISTs). Patients and Methods: This trial used a standard 3 + 3 design to determine the recommended phase II dose (RP2D). Additional patients were enrolled on an expansion cohort at the RP2D enriching for succinate dehydrogenase (SDH)-deficient GISTs to explore potential efficacy. Results: The trial enrolled nine patients in the dose-escalation cohort and 14 in the dose-expansion cohort including six with SDH-deficient GISTs. Imatinib 400 mg daily with binimetinib 45 mg twice daily was established as the RP2D. Dose-limiting toxicity (DLT) was asymptomatic grade 4 creatinine phosphokinase (CPK) elevation. The most common non-DLT grade 3/4 toxicity was asymptomatic CPK elevation (69.6%). Other common ≥grade 2 toxicities included peripheral edema (17.4%), acneiform rash (21.7%), anemia (30.4%), hypophosphatemia (39.1%), and aspartate aminotransferase (AST) increase (17.4%). Two serious adverse events occurred (grade 2 dropped head syndrome and grade 3 central retinal vein occlusion). No unexpected toxicities were observed. Limited clinical activity was observed in KIT-mutant GIST. For SDH-deficient GISTs, one of five had confirmed RECIST1.1 partial response (PR). The median progression-free survival (mPFS) in patients with SDH-deficient GIST was 45.1 months [95% confidence interval (CI), 15.8–not estimable (NE)]; the median overall survival (mOS) was not reached (95% CI, 31.6 months–NE). One patient with a refractory metastatic SDH-deficient GIST had an exceptional pathologic response and durable clinical benefit. Conclusions: The combination of imatinib and binimetinib is safe with manageable toxicity and has encouraging activity in SDH-deficient but not imatinib-refractory KIT/PDGFRA-mutant GISTs. The observed clinical benefits provide a motivation for a larger trial of the combination strategy in SDH-deficient GISTs.