Miracle-dz/UniFAR-FTUs huggingface dataset:
Reprogramming of Plant Central Metabolism in Response to Abiotic Stresses: A Metabolomics View

Abiotic stresses rewire plant central metabolism to maintain metabolic and energy homeostasis. Metabolites involved in the plant central metabolic network serve as a hub for regulating carbon and energy metabolism under various stress conditions. In this review, we introduce recent metabolomics techniques used to investigate the dynamics of metabolic responses to abiotic stresses and analyze the trend of publications in this field. We provide an updated overview of the changing patterns in central metabolic pathways related to the metabolic responses to common stresses, including flooding, drought, cold, heat, and salinity. We extensively review the common and unique metabolic changes in central metabolism in response to major abiotic stresses. Finally, we discuss the challenges and some emerging insights in the future application of metabolomics to study plant responses to abiotic stresses.
Proinflammatory Endothelial Phenotype in Very Preterm Infants: A Pilot Study

Very preterm infants are exposed to prenatal inflammatory processes and early postnatal hemodynamic and respiratory complications, but limited data are available about the endothelial effect of these conditions. The present pilot study investigates the perinatal endothelial phenotype in very preterm infants (VPIs) and explores its predictive value on neonatal mortality and hemodynamic and respiratory complications. Angiopoietin 1 (Ang-1), Ang-2, E-selectin, vascular adhesion molecule 1 (VCAM-1), tissue factor (TF), and endothelin 1 (ET-1) concentrations were tested in first (T1), 3rd (T2), and 7–10th (T3) day of life in 20 VPIs using Luminex technology and compared with 14 healthy full-term infants (FTIs). Compared to FTIs, VPIs had lower Ang-1 at T1 and T2; higher Ang-2 at T1, T2, and T3; higher Ang-2/Ang-1 ratio at T1, T2, and T3; lower E-selectin at T1, T2, and T3; higher VCAM-1 at T1; higher TF at T2. No differences in concentrations were found in neonatal deaths. VPIs with hemodynamic or respiratory complications had higher Ang-2 at T3. Perinatal low Ang-1 and high Ang-2 associated with high VCAM-1 and TF in VPIs suggest a proinflammatory endothelial phenotype, resulting from the synergy of a pathological prenatal inheritance and a premature extrauterine transition.
Diagnostic Value of hTERT mRNA and in Combination With AFP, AFP-L3%, Des-γ-carboxyprothrombin for Screening of Hepatocellular Carcinoma in Liver Cirrhosis Patients HBV or HCV-Related

Diagnosis of hepatocellular carcinoma (HCC) in early-stage, to give an effective treatment option and improve quality of life for cancer patients, is an important medical mission globally. Combination of AFP with some biomarkers may be more supportive in both diagnosis and screening of HCC, but the range value of these markers can be applied as daily markers were unclearly. In some studies, human telomerase reverse transcriptase (hTERT mRNA) was reported as an advantage marker to diagnose cancer. The present study identified serum of 340 patients that were infected chronic hepatitis B virus or hepatitis C virus and divided in 2 groups including Hepatocellular carcinoma (HCC) and liver cirrhosis (LC) to measure their values of hTERT mRNA, AFP, AFP-L3%, and DCP, as well as combination of them. As a result, the concentration of hTERT mRNA, AFP, AFP-L3%, and DCP in HCC groups were significantly higher than that in LC group (P < .01). For detecting HCC, hTERT mRNA had sensitivity of 88% and specificity of 96% (at the cutoff value of 31.5 copies/mL), AFP sensitivity of 73% and specificity of 92% (at the cutoff value of 5.1 ng/mL), AFP-L3% sensitivity of 69% and specificity of 90% (at the cutoff value of 1.05%), DCP sensitivity of 82% and specificity of 92% (at the cutoff value of 29.01 mAU/mL). The largest area under the curve (AUC) of combination hTERT mRNA with DCP was 0.932 (sensitivity of 98.2% and specificity of 88.2%). New combination of DCP with hTERT mRNA gave a useful choice for screening of HCC in chronic HBV or HCV patients associated liver cirrhosis.
Sexual Dimorphism of Dexamethasone as a Prophylactic Treatment in Pathologies Associated With Acute Hypobaric Hypoxia Exposure

Dexamethasone can be taken prophylactically to prevent hypobaric hypoxia-associated disorders of high-altitude. While dexamethasone-mediated protection against high-altitude disorders has been clinically evaluated, detailed sex-based mechanistic insights have not been explored. As part of our India-Leh-Dexamethasone-expedition-2020 (INDEX 2020) programme, we examined the phenotype of control (n = 14) and dexamethasone (n = 13) groups, which were airlifted from Delhi (∼225 m elevation) to Leh, Ladakh (∼3,500 m), India, for 3 days. Dexamethasone 4 mg twice daily significantly attenuated the rise in blood pressure, heart rate, pulmonary pressure, and drop in SaO2 resulting from high-altitude exposure compared to control-treated subjects. Of note, the effect of dexamethasone was substantially greater in women than in men, in whom the drug had relatively little effect. Thus, for the first time, this study shows a sex-biased regulation by dexamethasone of physiologic parameters resulting from the hypoxic environment of high-altitude, which impacts the development of high-altitude pulmonary hypertension and acute mountain sickness. Future studies of cellular contributions toward sex-specific regulation may provide further insights and preventive measures in managing sex-specific, high-altitude–related disorders.
Interacting water, nutrients, and shrub age control steppe grass‐on‐shrub competition: Implications for restoration

Understanding the factors that control grass – shrub interactions and coexistence is critical to the design of dryland management and restoration strategies. Using Stipa tenacissima tussocks as adult neighbor and Olea europaea var. sylvestris as target woody species, we performed a 5-year-long, large-scale manipulative experiment to investigate the independent and combined effects of water and nutrient availability, and the modulating effects of O. europaea ontogeny on the net outcome of grass – shrub interaction. At two contrasting dryland sites, we conducted experimental plantings of Olea seedlings on two microsites: contiguous to a Stipa tussock ( Stipa microsite) and on the inter-tussock bare-soil areas (Open microsite), and manipulated resource availability by combining nutrient and water addition. The experiment followed a full factorial design, with all treatment combinations applied at each site. We monitored survival, height, and stem basal diameter of Olea individuals over a study period of 5 years. All across the wide range of conditions
MARKETING MIX STRATEGY FORMULATION AND CREATING VALUE CO-CREATION IN SCHOOL EDUCATION

This study aims to describe internal and external factors with the 7P marketing mix concept at SMA Bogor Raya, define the gap between input and output from internal and external factors in the marketing strategy of SMA Bogor Raya, evaluate the 7P services marketing mix concept applied by the marketing unit of SMA Bogor Raya. This approach to study requires extensive relevant searches, business management, and educational databases on value-based marketing and co-creation. This study uses a qualitative research approach. The type of research used in this research is a case study. Subjects in this study were vital informants with a total of 7 informants. The research field of educational marketing, value, and co-creation is still at a relative pioneer stage. Much research is still to be carried out, both from problem identification and a strategic perspective. To increase public interest in school education services, a mixed market strategy can be applied by combining 7Ps consisting of product, price, place, promotion, people, process, and physical evidence. Although there is no master plan for implementing the marketing mix at SMA Bogor Raya, the strategy has been implemented.BAHASA INDONESIA ABSTRACTPenelitian ini bertujuan untuk mendeskripsikan faktor internal dan eksternal dengan konsep bauran pemasaran 7P di SMA Bogor Raya, menentukan gap antara input dan output dari faktor internal dan eksternal dalam strategi pemasaran SMA Bogor Raya, mengevaluasi konsep bauran pemasaran jasa 7P yang diterapkan oleh unit pemasaran SMA Bogor Raya. Pendekatan studi ini membutuhkan pencarian relevan yang ekstensif, manajemen bisnis, dan basis data pendidikan tentang pemasaran dan kreasi bersama berbasis nilai. Penelitian ini menggunakan pendekatan penelitian kualitatif. Jenis penelitian yang digunakan dalam penelitian ini adalah studi kasus. Subjek dalam penelitian ini adalah informan vital dengan jumlah informan sebanyak 7 orang. Bidang penelitian pendidikan pemasaran, nilai, dan co-creation masih pada tahap perintis relatif. Banyak penelitian yang masih harus dilakukan, baik dari identifikasi masalah maupun perspektif strategis. Untuk meningkatkan minat masyarakat terhadap layanan pendidikan sekolah, strategi pasar campuran dapat diterapkan dengan menggabungkan 7P yang terdiri dari produk, harga, tempat, promosi, orang, proses, dan bukti fisik. Meski belum ada masterplan penerapan bauran pemasaran di SMA Bogor Raya, namun strategi sudah diterapkan.
Enjoying the fruit of development? Working conditions and the earnings of low-skilled internal migrants in China across two decades (1993–2015)

ABSTRACT China’s unprecedented economic growth in recent decades can largely be attributed to millions of migrant labourers that made China the ‘world’s factory’. It is well documented, however, that migrant labourers experience discrimination in the labour market. Despite China’s phenomenal economic growth, few studies have investigated whether the working conditions of low-skilled migrants have improved. In this study, we ask: in the past two decades, have disparities in working conditions and earnings between low-skilled rural-to-urban migrant workers and their urban counterparts decreased? We contrast the working conditions and earnings between migrants and urban residents who lack a high school degree and age 18–55 years using panel data, the China Health and Nutrition Survey (1993–2015), and individual fixed effects models to account for selection problems. Findings indicate that over a span of twenty years migrants and urban residents had similar trends in employment and earned incomes, but migrants’ weekly working hours and tendency to work more than legal work hours increased more than urban residents’. Working longer without earning more suggests persistent social inequality in Chinese society in general and deeper injustice among migrant workers.
Full-field optical analysis of 3D printing composite for defect detection

Nowadays, due to the competitive economic scenario, industries ever more need to focus on manufacturing speed, increasing efficiency and steady quality. More industrial sectors see additive manufacturing (AM) as a possible way to enhance their processes and increase production efficiency. Thanks to its versatility the Fused Deposition Modelling (FDM), also known as Fused Filament Fabrication (FFF) technique, is one the most attractive processes in Industry 4.0 paradigm. This technique, thanks to its low-cost, is spreading widely in industrial sectors, from biomedical to aerospace to cite some. In this frame a valid solution is to use composite materials Among many, particular attention is paid to thermoplastic systems based on polyether-ether-ketone (PEEK) reinforced with short carbon fibre (CF). The PEEK is a high-performance semi-crystalline thermoplastic polymer that belongs to the polyaryl-ether-ketone family (PAEK). 3D printing, being a novel technology, it must be validated by understanding the behaviours of components and structures. At this purpose, we used different optical techniques for analysing advanced short fibre composites realized by 3D printing. Different CFR-PEEK samples with short carbon fibre at 10% by weight were realized by the FFF technique and characterized in terms of failure mode and mechanical behaviour. Optical tools have been used to retrieve full-field data and expand information about the mechanical behaviour of the investigated material, i.e. 2D Digital Image Correlation (2D-DIC) and Electronic Speckle Pattern Interferometry (ESPI), Optical Microscopy (OM) and Scanning Electron Microscopy (SEM).
Robust increase in population exposure to heat stress with increasing global warming

Extreme heat, particularly if combined with humidity, poses a severe risk to human health. To estimate future global risk of extreme heat with humidity on health, we calculate indicators of heat stress that have been commonly used: the Heat Index, the Wet-Bulb Globe Temperature and the Wet-Bulb Temperature, from the latest Climate Model Intercomparison Project (CMIP6) projections. We analyse how and where different levels of heat stress hazards will change, from severe to deadly, and how results are sensitive to the choice of the index used. We evaluate this risk at country-level and use population and GDP | PPP growth scenario to estimate the vulnerability of each nation. Consistent with previous studies, we find that South and East Asia, and the Middle-East, are highly exposed to heat stress hazards, and that this exposure increases by 20%–60% with global mean temperature change from 1.5 to 3 ∘C. However, we also find substantial increases in heat health risk for some vulnerable countries with less adaptive capacity, such as West Africa, and Central and South America. For these regions, about 20 to more than 50% of the population could be exposed to severe heat stress each year on average, independent of the index used. For global warming of 3∘, European countries and the USA will also be exposed several times per year to conditions with daily mean heat stress level equal to the maximum heat stress of the 2003 heat wave.
Synthesis and Characterization of a Cationic Thiomer Based on Ethyl Cellulose for Realization of Mucoadhesive Tablets and Nanoparticles

Purpose Ethyl cellulose (EC) based nanoparticles are being extensively studied for their ability to achieve prolonged drug release and improve drug stability. Within this study, the thiolation of unmodified EC using cysteamine as a ligand was carried out to design nanoparticles with mucoadhesive properties and comparatively strong lipophilic properties. Methods The thiolation was performed via oxidation and reductive amination, whereas the nanoparticles were generated via the ionic gelation followed by the precipitation method. Results The number of free thiol groups on EC-cysteamine was in the range of 210–261 µmol per gram of polymer. Tablets based on EC-cysteamine demonstrated mucoadhesive properties 16.7-fold improved compared with those comprising unmodified EC. The mean diameter of the particles was in the range of 94–123 nm and the zeta potential was determined to be −7.97 to −14.70 mV. The nanoparticles remained attached to porcine intestinal mucosa for up to 36% after 3 h of incubation. The formation of nanoparticles improved the stability of EC-cysteamine conjugate against cellulase and provided a zero-order release. Moreover, both EC-cysteamine and the nanoparticles did not show any pronounced cytotoxicity. Conclusion Accordingly, EC-cysteamine nanoparticles could be a specific type of nanoparticulate delivery system with mucoadhesive properties. The amount of free thiol groups within EC-cysteamine nanoparticles together with their lipophilic properties could be further modified and modulated for a desired release behavior.
Vaginal cytology in mares in the different stages of the estral cycle associated with the seasons of the year

The reproductive biotechnologies directly assist in the development of different methods of reproductive evaluation. The objective of this study was to develop a new method to determine the estrous cycle phase in equine females through vaginal cytology throughout the seasons, as well as to establish an estimate of inflammatory cells present in the vagina. Six mares were evaluated at different ages without a defined breed and reproductive activity and were subjected to ultrasound evaluation of the reproductive system and cytological analysis of the vaginal region. In the summer, there was a predominance of keratinized epithelial cells in the estrus and intermediate in the diestrus. In the autumn, there was a predominance of keratinized cells in estrus and parabasal cells in estrus and diestrus. In anestrus (winter), a greater number of parabasal cells was identified in relation to the other cell types. In estrus (spring), there was a predominance of parabasal and intermediate cells. Conversely, in diestrus, parabasals were found in greater numbers. The evaluation of inflammatory cells of the vaginal epithelium of mares showed greater activity in the summer; however, there are no reference values for healthy animals in the literature, and it is necessary to conduct studies on the subject. It is concluded that there is an influence of cyclicity on the vaginal epithelium of the equine species, varying according to the season. Additionally, vaginal cytological evaluation is an important complementary tool in the diagnosis of vaginitis in mares, requiring further research on the subject.
Establishment of a Landscape of UPL5-Ubiquitinated on Multiple Subcellular Components of Leaf Senescence Cell in Arabidopsis

Catabolism of macromolecules is a major event in senescent cells, especially involving proteolysis of organelles and abnormally aggregated proteins, circulation of nutrients, and precise control of intracellular environmental balance. Proteasomes are distributed in the nucleus and cytoplasm; however, proteasomes in organelles are limited. In this study, multi-omics proteomic analyses of ubiquitinated proteins enriched by using antibody against “di-Gly-Lys” via a free labeling were used to investigate the global changes of protein levels and ubiquitination modification levels of upl5 mutant relative to wild-type plant; subcellular localization analysis of UPL5 was found to be located in the nucleus, cytoplasm, and plastid within the cell; and the direct lysine site patterns of UPL5 were screened by the H89R substitution in the tagged ubiquitinated assay. It suggests that UPL5 acting as a candidate of organelle E3 ligase either in the nucleus or cytoplasm or plastid modifies numerous targets related to nuclear transcription and plastid photosynthesis involving in Ca2+ and hormone signaling pathway in plant senescence and in response to (a)biotic stress protection.
Fatigue Analysis of a Light Truck Rear Axle Based on Virtual Iteration Method

Firstly, the MBD (multi-body dynamics) model of the whole vehicle is built according to the measured vehicle parameters. Then, the road spectra acquisition test is carried out. Under the test road conditions, the acceleration, displacement, and force signals of multiple positions of the whole vehicle are collected. Taking the road test signals as the iterative target and the vehicle MBD model as the iterative carrier, the vehicle equivalent excitation is obtained by using the VIM (virtual iteration method). By applying the equivalent excitation obtained by VIM to the vehicle MBD model, the load spectra of key points of the driving rear axle are obtained. The accuracy of the model is verified by comparing the measured signals with the iterative signals, and the reliability of the key points load spectra is enhanced. Secondly, the FEA (finite element analysis) model of the rear axle is built, and the static analysis results of each key point under unit load are obtained with the help of FEA software. Thirdly, the FEA results, key points load spectra, and material fatigue characteristic curve of the rear axle are input into the fatigue software to conduct the fatigue simulation of the rear axle. According to the fatigue analysis results, the position where the fatigue life does not meet the set goal is accurately located. Finally, the structure is optimized based on the above results. The fatigue life evaluation of the optimized rear axle shows that its durability has been significantly improved.
Land use/land cover changes in the central part of the Chitwan Annapurna Landscape, Nepal

Background Land use/land cover assessment and monitoring of the land cover dynamics are essential to know the ecological, physical and anthropogenic processes in the landscape. Previous studies have indicated changes in the landscape of mid-hills of Nepal in the past few decades. But there is a lack of study in the Chitwan Annapurna Landscape; hence, this study was carried out to fill in study gap that existed in the area. Methods This study evaluates land use/land cover dynamics between 2000 to 2020 in the central part of the Chitwan Annapurna Landscape, Nepal by using Landsat images. The Landsat images were classified into eight different classes using remote sensing and geographic information system (GIS). The accuracy assessment of classified images was evaluated by calculating actual accuracy, producer’s accuracy, user’s accuracy and kappa coefficient based on the ground-truthing points for 2020 and Google Earth and topographic maps for images of 2010 and 2000. Results The results of land use/land cover analysis of Landsat image 2020 showed that the study area was composed of grassland (1.73%), barren area (1.76%), riverine forest (1.93%), water body (1.97%), developed area (4.13%), Sal dominated forest (15.4%), cropland (28.13%) and mixed forest (44.95%). The results of land cover change between 2000 to 2020 indicated an overall increase in Sal dominated forest (7.6%), developed area (31.34%), mixed forest (37.46%) and decrease in riverine forest (11.29%), barren area (20.03%), croplands (29.87%) and grasslands (49.71%). The classification of the images of 2000, 2010 and 2020 had 81%, 81.6% and 84.77% overall accuracy, respectively. This finding can be used as a baseline information for the development of a proper management plan to protect wildlife habitats and forecasting possible future changes, if needed.
PENGEMBANGAN MEDIA KOMIK UNTUK MENINGKATKAN HASIL BELAJAR SISWA KELAS IV

Latar belakang dari penelitian ini adalah kreativitas guru kurang dalam menggunakan media pembelajaran dan belum tersedianya media pembelajaran. Jenis penelitian yang digunakan adalah Research and development (RnD) dan menggunakan model ADDIE (Analysis, Design, Development, and Evaluation). Tujuan dari penelitian ini adalah untuk mengukur peningkatan pada siswa setelah menggunakan media komik berbasis media cetak. Penelitian menggunakan instrument berupa wawancara, observasi, lembar angket validasi ahli materi, lembar angket validasi ahli media, lembar angket validasi praktisi, angket respon siswa, dan soal test untuk mengukur pengetahuan siswa. Penelitian ini dilakukan di SDN 5 Siki, dilihat dari tingkat kelayakan media komik berbasis media cetak ini pada penelitian ini didasarkan pada validasi yang dilakukan oleh ahli materi, ahli bahasa,ahli media dan ahli praktisi. Yang pertama dilihat dari aspek angket validasi ahli materi pembelajaran memperoleh nilai 91,66%,kriteria kevalidan jika dicocokkan yaitu sangat valid sehingga media dapat digunakan dalam proses pembelajaran. Selanjutnya dari aspek angket ahli media pembelajaran memperoleh nilai 80,76%, kriteria kevalidan jika dicocokkan yaitu valid, selanjutnya dari aspek angket ahli bahasa memperoleh nilai 87,5%, kreteria kevalidan jika dicocokan yaitu valid sehingga media dapat digunakan dalam proses pembelajaran dan yang terakhir yaitu ditinjau dari aspek angket ahli praktisi guru kelas IV SDN 5 Siki memperoleh nilai 94,4%, kriteria kevalidan jika dicocokkan yaitu sangat valid, sehingga media dapat digunakan dalam proses pembelajaran. Sedangkan berdasarkan nilai pretest dan posttest digunakan untuk mengukur peningkatan pengetahuan siswa tentang gunung meletus. Nilai pretest memperoleh rata-rata 50,85% dan nilai post test memperoleh nilai 90,71% . Dari perbandingan nilai tersebut dapat diartikan bahwa siswa mengalami peningkatan sebanyak 39,86%. Nilai pree test dan post test jika dihitung menggunakan N-Gain nilainya meningkat sebanyak 0,80 termasuk kedalam nilai tinggi.