Miracle-dz/UniFAR-FTUs huggingface dataset:
The Influence of BPJS Health Payment and Service Method for Satisfaction of BPJS Health Members in South Tangerang

The research aims to find out: Does the payment method set by BPJS in South Tangerang attract public interest in paying BPSJ compulsory health contributions. Does maximum service at South Tangerang BPJS Health affect the satisfaction of BPJS Health members. Do the right payment methods and services together affect the satisfaction of members of the Health BPJS in South Tangerang. The research method is a quantitative method that is a method that emphasizes more on the aspect of measuring objectively the social phenomenon that is the user community of BPJS health services in South Tangerang. Data collection methods use primary data. The analysis used in this study is multiple linear regression analysis, namely by calculating the SPSS through Validity Testing, reliability testing, testing traditional assumptions, and multiple linear regression analysis. The results of this study indicate that the Payment Method has a positive effect on the satisfaction of members of the Health BPJS, the Health Service BPJS has a positive effect on the satisfaction of the Health BPJS members, the payment method and the Health BPJS Services jointly affect the satisfaction of the Health BPJS members.
Improvement of High-throughput Genotype Analysis After Implementation of a Dual-curve Sybr Green I-based Quantification and Normalization Procedure

The ability to rapidly genotype a large number of individuals is the key to any successful marker-assisted plant breeding program. One of the primary bottlenecks in high-throughput screening is the preparation of DNA samples, particularly the quantification and normalization of samples for downstream processing. A rapid and simple Sybr Green I-based quantification procedure that can be performed in a 96-well format is outlined. In this procedure, a dual standard curve method is used to allow better resolution of dilute samples and to reduce fluorescence value variation between samplings. A method to quickly normalize samples, and the importance of normalization, is also explored. We demonstrate that successful fragment amplification of a Theobroma grandiflorum (Willd ex Spreng) Schum. population is increased from 70% to 98% when each DNA extract is quantified and normalized as opposed to quantifying only a subset and normalizing all the samples based on the average of that subset. Improved microsatellite amplification was also observed among individuals in the monocot genus Phaedranassa Herb. ssp. Additionally, when our normalization method is applied to a Persea americana Mill. population, 97% of the samples normalized to 4 ng- μL 1 amplify at least three of six microsatellite regions, whereas only 30% of the samples below 4 ng μL 1 (i.e., samples that could not be normalized) amplify at least three regions. We describe an undemanding method to quantify and normalize a large number of samples, which can be done manually or can be automated.
Molecular Cloning: A Laboratory Manual

Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Mol
Salt stress induced glycine-betaine accumulation with amino and fatty acid changes in cyanobacterium Aphanothece halophytica.

A halotolerant cyanobacterium Aphanothece halophytica was grown under various NaCl concentrations from 0.5 – 3.0 M and pH from 6.5 – 10.5. The optimal condition for cell growth was 0.5 M NaCl and pH 9.5. The relationship of cell growth, cell morphology, intracellular solute, and amino acid composition was investigated. Under salt stress conditions, cell growth was reduced when NaCl was increased and cell size was increased with high glycine-betaine accumulation. Ion contents of Na, K, NH4, and NO3 were not significantly different. Under both non-stress and salt stress conditions, glutamine was the major amount of amino acid. However, glutamine, aspartate, proline and glutamate were increased under salt stress conditions. Exposure of A. halophytica cells to salt stress conditions significantly enhanced saturated fatty acid composition.
Bioenergetics Modeling: Progress since the 1992 Symposium

Abstract A symposium held at the annual meeting of the American Fisheries Society in Rapid City, South Dakota, in 1992 provided an overview of bioenergetics modeling and led to the publication of a series of papers in the Transactions of the American Fisheries Society the next year. Since then, Wisconsin Sea Grant has published a new version of its bioenergetics software (Fish Bioenergetics 3.0) and a manual to facilitate use of that model. Review of the more recent literature reveals continuing development and application of this modeling approach. A symposium held at the annual meeting in Madison, Wisconsin, in 2004 provided an opportunity for reporting progress and concerns about the “Wisconsin model. ” This special section stems from the papers presented at that meeting.
Effect of Planting System on Productivity, Dry-matter Partitioning and Carbohydrate Content in Above- ground Components of 'Flordaprince' Peach Trees

Vegetative growth, fruit yields, and dry matter partitioning within above-ground components were assessed during three growing seasons for trees of an early ripening peach ( Prunus persica L. Batsch 'Flordaprince' on GF 677 rootstock) trained either to a free standing central leader (930 trees/ha) or to Y shape (1850 trees/ha). Individual trees trained to central leader gave higher fruit yield, had a significantly greater leaf area and accumulated more dry mass in above-ground components per tree than Y shape trees. The training systems did not differ in terms of yield efficiency (yield per trunk cross-sectional area) and leaf area index (LAI), but Y shape trees had a higher harvest index and fruit dry mass per ground area than central leader. Four years after planting, Y shape had 35% higher yield per hectare than central leader. The relative contribution of 1-year-old wood, shoot and leaf to the dry mass of the tree decreased with tree age. Four years after planting the dry matter partitioned to the >1-year-old wood components represented 60% of the total tree mass (excluding fruit) in both the training systems. Central leader trees had the highest relative vegetative growth rate during stage III of fruit development. Most starch depletion occurred from dormancy to pit hardening from the canopy main storage pools (>1-year-old wood), and was higher for central leader than Y shape trees. For the ease of management and the high crop efficiency, the Y shape can be successfully used for peach high density planting systems.
Comparative Genomic Analysis and Evolutionary Study of CD4 Antigen

CD4 antig en is important to act as th e prim ary receptor for the HIV. In order to understand th e human CD4 gene and to determine its evolutionary aspects, we characterized this gene in detail in six different organisms. A comparative study was made of nucleotide length variations, intron and exon sizes and number variations, differential compositions of coding to non-coding bases, etc., to look for similarities/dissimilarities in the CD4 gene across all six taxa. Phylogenetic analysis showed the pattern found in other genes, as Homo sapiens and Pan troglodytes were placed in a single clade, and Rattus norvegicus and Mus musculus in another. We further focused on the two primates and aligned the amino acid sequences; there were small differences between humans and chimpanzees; both were more different from the other organisms.
A MethodologicalApproachto the Predictionof AnticancerDrugEffect in Humans1

Tumor cells from animals and humans were treated with drugs under tissue culture conditions. Tumor cells from the sensitive L1210 model were studied first. A dose-response curve was derived between drug exposure and subsequent cytotoxicity in L1210. The concentration of drug and dura tion of exposure were factors critical to the subsequent development of in vitro cytotoxicity. The in vitro dosage which effected 50% leukemic cell death in L1210 cells correlated with reported in vivo drug levels. Other tumor models and human neoplastic cells were studied at this dosage level. A good correlation was noted in these studies between the in vivo responsiveness and the in vitro chemo therapy results in both animals and humans. It was sug gested by these results that it may be possible to predict cancenicidal drug activity for individual neoplasms by as saying the tumor cells in vitro for drug sensitivity.
LABORATORY EVALUATION OF SOME BOTANICALS AGAINST DIAMOND BACK MOTH, Plutella xylostella L. (LEPIDOPTERA: PLUTELLIDAE)

The present study explores certain plants of Euphorbiaceae family for their insecticidal property against Plutella xylostella L. by using leaf dip method as bioassay technique. Of the 15 plants tested, nine had potential insecticidal property. This study could bring forth some plant species, which are potentially insecticidal against P. xylostella viz., Euphorbia antiquorum, E. pulcherrima, Croton bonplandianum, Jatropa curcas , J. gossypifolia, Breynia officinalis and Ricinus communis.
Improving statistical practice in psychological research: Sequential tests of composite hypotheses

Statistical hypothesis testing is an integral part of the scientific process. When employed to make decisions about hypotheses, it is important that statistical tests control the probabilities of decision errors. Conventional procedures that allow for error-probability control have limitations, however: They often require extremely large sample sizes, are bound to tests of point hypotheses, and typically require explicit assumptions about unknown nuisance parameters. As a consequence, the issue of proper error-probability control has frequently been neglected in statistical practice, resulting in a widespread reliance on questionable statistical rituals. In this thesis, I promote an alternative statistical procedure: the sequential probability ratio test (SPRT). In three articles, I implement, further develop, and examine three extensions of the SPRT to common hypothesis-testing situations in psychological research. In the first project, I show that the SPRT substantially reduces required sample sizes while reliably controlling error probabilities in the context of the common t-test situation. In a subsequent project, I seize on the SPRT to develop a simple procedure that allows for statistical decisions with controlled error probabilities in the context of Bayesian t tests. Thus, it allows for tests of distributional hypotheses and combines the advantages of frequentist and Bayesian hypothesis tests. Finally, I apply a procedure for sequential hypothesis tests without explicit assumptions about unknown nuisance parameters to a popular class of stochastic measurement models, namely, multinomial processing tree models. With that, I demonstrate how sequential analysis can improve the applicability of these models in substantive research. The procedures promoted herein do not only extend the SPRT to common hypothesis-testing situations, they also remedy a number of limitations of conventional hypothesis tests. With my dissertation, I aim to make these procedures available to psychologists, thus bridging the gap between the fields of statistical methods and substantive research. Thereby, I hope to contribute to the improvement of statistical practice in psychology and help restore public trust in the reliability of psychological research.
Wireless Powering of Next-Generation Left Ventricular Assist Devices (LVADs) Without Percutaneous Cable Driveline

This article deals with the design of a wireless powering system for a left ventricular assist device (LVAD). The modern LVAD is mainly a miniaturized blood pump attached to the heart and is driven by an electric motor powered by a cable driveline that exits the patient’s body through the skin to be connected with an external battery. The driveline is a portal to the exterior environment and this can cause frequent and severe infections. The goal of this article is the elimination of the cable link between the interior and the exterior of the human body. To this aim, an innovative powering design of the LVAD is proposed adopting the wireless power transfer (WPT) technology based on magnetic resonant coupling. The main challenges in the design of a WPT system for LVAD application are the considerable implantation depth (5–10 cm) and the relatively high power ( $\approx 5$ W) required by the device to operate continuously. Two different design solutions are proposed: 1) a subcutaneous receiving coil connected to the LVAD by a short driveline completely inside the human body and 2) an LVAD with a receiving circuit and coil, both integrated on it.
Strengthening Public Value Through Reinforcing Entrepreneurial Orientation and Organization: Preliminary Step Toward Entrepreneurial Universities

Higher education institutions as public sectors are considered by some to be unable to provide optimal impact in meeting the needs of industries and businesses. This condition raises demands to operate entrepreneurially, leading to commercialization of research result to encourage the acceleration of innovation. Higher education institutions must be encouraged towards an entrepreneurial state of mind. In order to take a preliminary stage, this paper proposed model using 4 variables are: organization, learning organization, entrepreneurial orientation and public value. Respondents participate in this research 146 people from public and private universities. Almost all of hypotheses testing are proven to have significant influence using PLS (Partial Least Square). This study successfully proved that organizations can improve learning orientation and entrepreneurial orientation among middle manager in higher education institutions. This research also has succeeded in proving that entrepreneurial orientation and commitment to learning have been proven to improve public value (performance). In the future, this modelling can be used as a reference for conducting field studies, so that concrete facts in the field on the “possibility to achieve entrepreneurial university in Indonesia” can be obtained.
Modelling fuel consumption of fishing vessels for predictive use

This is an author produced pre-print pdf. The article has been accepted for publication in ICES Journal of Marine Science, published by Oxford University Press. Available at: ICES J. Mar. Sci. (January/February 2015) 72(2):708-719. doi:10.1093/icesjms/fsu084. First published online: June 10, 2014
Validation of the Korean version of Feedback Environment Scale for
 Students(K-FESS)

T he main p ur p ose of this study was to validate the Korean version of the Feedback Environment Scale for Students(K-FESS). T he feedback environment is a contextual as p ect of the feedback p rocess and the feedback-oriented culture influences considerably the learner ' s acce p tance of feedback and whether they reflect this in their behavior. N evertheless, there are few research related to the effects of the feedback environment in the classroom assessment of Korea. T herefore, in this study, we utili z ed the Feedback Environment Scale for Students(Steelman et al., 2004), and validated it for use with elementary, middle, and high school students in Korea. We also investigated construct e q uivalence of this scale across the school levels. For this study, reliability analysis, confirmatory factor analysis ( C FA), correlation analysis, and multi- G rou p C FA were a pp lied to check the p sychometric p ro p erties of the K-FESS. First, the K-FESS with 23 items was acce p ted that only one item had been excluded from the original version through translation and content validation p rocedure. Second, the five-factor solution was p roduced by C FA and it exhibited good reliability and validity. T hird, the construct e q uivalence of the K-FESS across the school levels was confirmed. T his means that the K-FESS can be used as a common scale for all school levels. Fourth, the latent mean analyses revealed that the elementary school students had significantly higher feedback environment p erce p tions than those in other school levels. H owever the latent mean differences between middle and high school students were inconsistent across the subscales. Finally, im p lications for the a pp lied use of the K-FESS in Korea are discussed.
Prominent Local Representation for Dynamic Textures Based on High-Order Gaussian-Gradients

Understanding dynamic textures (DTs) is a challenge in various computer vision applications due to the negative impacts of noise, changes of environment, illumination, and scales on capturing turbulent characteristics. In this work, we propose an efficient shallow framework for DT representation by addressing the following novel concepts. First, it is the first time in DT analysis that 2D/3D Gaussian-gradient filterings are taken into account as a pre-processing step to point out robust components against those influences in effect. Second, high-order partial derivatives of the Gaussian kernels and their informative magnitudes are exploited to forcefully capture multi-order Gaussian-gradient features. Third, these gradient kernels are investigated in multi-scale analysis of different orders and standard deviations in order to enrich more useful scale-gradient information. Finally, the obtained complementary components are shallowly encoded using a simple local operator to construct robust descriptors of High-order 2D/3D Gaussian-gradient-based Features ($\mathrm{HoGF}^{2D/3D}$) against the well-known issues of DT description. Experiments for DT classification on various benchmarks have validated the interest of our approach since its performance is comparable to state-of-the-art results, including that of deep-learning methods, while it only has a small dimension.
Na + ,K + ,2Cl - -cotransporter: A Novel Marker for Identifying Freshwater- and Seawater-type Mitochondria-rich Cells in Gills of the Euryhaline Tilapia, Oreochromis mossambicus

The abundance and location of the branchial Na + ,K + ,2Cl - -cotransporter (NKCC) were examined in freshwater- and seawater-adapted tilapia. Immunoblots revealed 4 bands with molecular masses centered at 282, 208, 122, and 105 kDa, respectively. Gill NKCC in tilapia is upregulated after seawater adaptation. Confocal laser scanning micrographs showed that in seawater-adapted tilapia, NKCC as well as Na,K-ATPase exhibited identical diffuse distribution confined to the basal portion of branchial mitochondria-rich cells. However, in freshwater-adapted tilapia, NKCC was displayed only in the apical region of Na,K-ATPase-immunoreactive cells. Polarized distribution of NKCC makes it a novel marker for recognizing freshwater- or seawater-type MR cells in euryhaline tilapia.
FOOD CONSUMED BY GREAT HORNBILL AND RHINOCEROS HORNBILL IN TROPICAL RAINFOREST, BUDO SU-NGAI PADI NATIONAL PARK, THAILAND

Great and Rhinoceros Hornbills are omnivorous. Food consumed by these two species was studied during the breeding season (January to July of 2003 and 2004) in tropical rainforest, Budo Su-Ngai Padi National Park. The results showed that the Great and the Rhinoceros Hornbills had similar duration of every phase in the breeding cycle, i. e. nest visiting, sealing, incubation, early nestling and late nestling. However, considering the entire breeding cycle, Great Hornbill had signifi cantly shorter cycle (110.6±6.7 days) than that of the Rhinoceros Hornbill (121.7±10.4 days). Total food consumption rate of Great Hornbill was signifi cantly less than (47.0 g/Obs.h.) that of the Rhinoceros Hornbill (62.5 g/Obs.h.), and the diet was comprised of fi non-fi g (41.5%) and animal (4.9%). Proportion of fi the diet of Rhinoceros Hornbill was also signifi cantly higher (72.3%), but similar proportion of non-fi g (23.5 %) and animal (4.2%). Of the total 21 species of non-fi g diet of both Great and Rhinoceros Hornbills in this study, Polyalthia sp. (family Annonaceae) and Aglaia spectabilis (family Meliaceae) dominated fruit food by weight, while millipede dominated animal food. There was no difference in diversity of non-fi g fruit species consumed during all phases between both hornbills except during late nestling. In general, the diet of Great Hornbill has signifi cantly higher diversity than that of Rhinoceros Hornbill. From similarity coeffi cient, it was indicated that they used the same food resources which were mainly available during the study period. This paper was presented at the 5 th International Hornbill Conference jointly organised by the National Parks Board (Singapore) and the Hornbill Research Foundation (Thailand), in Singapore on 22 nd -25 th March 2009.
EVALUATING INTERNAL VERSUS EXTERNAL CHARACTERS: PHYLOGENETIC ANALYSES OF THE ECHINOCONCHIDAE, BUXTONIINAE, AND JURESANIINAE (PHYLUM BRACHIOPODA)

Abstract Evolutionary relationships between the Echinoconchidae, Productidae, Buxtoniinae, and Juresaniinae (Phylum Brachiopoda, Order Productida) have been the subject of debate for the better part of a century. The original (Muir-Wood and Williams, 1965) and revised (Brunton et al., 2000) Brachiopoda volumes of the Treatise on Invertebrate Paleontology use markedly different classifications and emphasize different characters. The 1965 Treatise classification for these taxa primarily was based on internal features, especially the cardinal process; the revised Treatise (2000) relied on external ornament and shell shape. Multiple phylogenetic analyses (global parsimony, stratocladistics, nearest-neighbor) of 14 genera, representing all of the relevant subfamilies and outgroups, are in strong agreement that 1) the subfamily Buxtoniinae belongs in the family Echinoconchidae, not to the Productidae; 2) the subfamily Juresaniinae is more closely related to the Echinoconchinae than to the Buxtoniinae; and 3) that internal characters, such as a shafted cardinal process and anterio-medial position of the brachial valve adductor field, provide the best phylogenetic signal, and are synapomorphic for the Echinoconchidae. Jackknifing and Bremer Support corroborate these results.