Miracle-dz/UniFAR-FTUs huggingface dataset:
The contraceptive potential of Carica papaya seed on oestrus cycle, progesterone, and histomorphology of the Utero-ovarian tissue of adult wistar rats

Objective: The study’s goal was to ascertain the contraceptive effects of Aqueous extract of Carica papaya on female rats by assessing changes in the body weight, estrous cycle, serum progesterone level and the cyto-architecture of the Utero-ovarian tissue. Methods: We used twenty (20) healthy young Adult Female Albino rats. The study ran for 7 and 21 days, respectively. Each study group has their Experimental (treated 200mg/kg aqueous extract of Carica papaya seed extract) and Control group (n=5). We determined daily the phases and frequencies of the estrous cycles of the rats during the administration of the extract. We processed the utero-ovarian tissue for histological analysis, and we assessed serum progesterone level and the oestrus cycle pattern. Results: There was a significant increase in body and Ovarian weights after 21 days of treatment when compared to controls and those treated for 7 days. However, uterine weight reduced significantly (p<0.05), serum progesterone level decreased (p<0.05) in the treated rats, mostly in those submitted to 21 day-treatments; the ovary showed marked degeneration of the theca cells, granulosa and corpus luteum, and loss of mucin granules in the uterine tissues. Carica papaya administered for 7 and 21 days caused the animals to have more proestrus and diestrus phases as compared to the control animals. The estrous cycle became irregular, with prolonged diestrous and proestrus phase. Conclusion: The aqueous extract of Carica papaya seeds caused antifertility, anti-implantation, by a reduction in progesterone level, disruption of oestrus pattern and histological alteration of utero-ovarian tissue.
Current Perspective of Antiviral Strategies against COVID-19

COVID-19 was declared a pandemic by the World Health Organization on March 11, 2020. This novel coronavirus disease, caused by the SARS-CoV-2 virus, has resulted in severe and unprecedented social and economic disruptions globally. Since the discovery of COVID-19 in December 2019, numerous antivirals have been tested for efficacy against SARS-CoV-2 in vitro and also clinically to treat this disease. This review article discusses the main antiviral strategies currently employed and summarizes reported in vitro and in vivo efficacies of key antiviral compounds in use.
Early Detection and Prediction of Infection using Infrared Thermography

Helicobacter pylori infection causes gastritis and gastric ulcers and associated gastric cancer. Approximately 50% of the world's population H pylori infection. Developing countries with high prevalence rates. In most individuals, low socio economic means and infections are acquired during childhood. Affected family members at higher risk for childhood acquisi tion. The definitive routes of transmission are not clear evidence oral-oral, gastro-oral and fecal-oral routes of transmission. If left untreated, H pylori infection can last a lifetime. The disease is usually clinical and often occurs decades after the initial infection. H pylori infection can cause gastritis in children. Lesions, mucus-associated lymphoid lymphoma, and rarely, intes tinal metaplasia with/without gastric dysplasia.Synovial fluid aspirates were analyzed total knee arthroplasty by polymerase chain reaction in 50 patients with symptoms. Its presence indicates a bacterial infection called deoxyribonucleic acid. Syn ovial fluid samples were processed using a rapid bacterial degradation and extracts are amplified using protocol polymerase chain reaction and products analyzed including universal bacterial primers polymerase chain reaction deoxyribonucleic acid compound. A total of 32 samples were positive for bacterial infection with pre-polymerase chain reaction aspirates. We ana lyzed antimicrobial use in 509 episodes with microbiology laboratory report implications. Antibiotic stewardship. Most the rapeutic interventions occurred during phlebotomy and results by telephone after reporting gram stain release antimicrobial susceptibility data were limited impact on microbial management.A significant cause of hospitalization is leading to infection North America the second leading cause of death children and under after 1 year of malaria worldwide. Because of its global and human health impact, relatively few treatments exist. Paradoxically, there is a huge amount of information available on RSV replication during purification, RSV-induced mechanisms and social spread. RSV adds to the high burden of infection with existing chronic respiratory diseases.A major concern in clinical practice is vancomycin-resistant enterococci (VRE). Increased prevalence and their potential other bacteria to develop resistance to vancomycin (including methicillin-resistant Staphylococcus aureus) subjected them to closer scrutiny and intensive investigation. Colonialism was generally acquired high colonization rate of VRE in patients surrounding susceptible hosts.
Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment

Superparamagnetic iron oxide nanoparticles (SPIONs) are promising drug delivery carriers and hyperthermia agents for the treatment of cancer. However, to ensure their safety in vivo, SPIONs must be modified in order to prevent unwanted iron release. Thus, SPIONs were coated with silica layers of different morphologies: non-porous (@SiO2), mesoporous (@mSiO2) or with a combination of non-porous and mesoporous layers (@SiO2@mSiO2) deposited via a sol–gel method. The presence of SiO2 drastically changed the surface properties of the nanoparticles. The zeta potential changed from 19.6 ± 0.8 mV for SPIONs to −26.1 ± 0.1 mV for SPION@mSiO2. The Brunauer–Emmett–Teller (BET) surface area increased from 7.54 ± 0.02 m2/g for SPIONs to 101.3 ± 2.8 m2/g for SPION@mSiO2. All types of coatings significantly decreased iron release (at least 10 fold as compared to unmodified SPIONs). SPIONs and SPION@mSiO2 were tested in vitro in contact with human lung epithelial cells (A549 and BEAS-2B). Both nanoparticle types were cytocompatible, although some delay in proliferation was observed for BEAS-2B cells as compared to A549 cells, which was correlated with increased cell velocity and nanoparticles uptake.
Coordinated Activation of ARF1 GTPases by ARF-GEF GNOM Dimers Is Essential for Vesicle Trafficking in Arabidopsis

ARF-GEF GNOM of Arabidopsis occurs as a dimer and mediates coordinated activation of ARF1 GTPases required for membrane vesicle formation. Membrane trafficking maintains the organization of the eukaryotic cell and delivers cargo proteins to their subcellular destinations, such as sites of action or degradation. The formation of membrane vesicles requires the activation of the ADP-ribosylation factor ARF GTPase by the SEC7 domain of ARF guanine-nucleotide exchange factors (ARF-GEFs), resulting in the recruitment of coat proteins by GTP-bound ARFs. In vitro exchange assays were done with monomeric proteins, although ARF-GEFs form dimers in vivo. This feature is conserved across eukaryotes, although its biological significance is unknown. Here, we demonstrate the proximity of ARF1•GTPs in vivo by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy, mediated through coordinated activation by dimers of Arabidopsis (Arabidopsis thaliana) ARF-GEF GNOM, which is involved in polar recycling of the auxin transporter PIN-FORMED1. Mutational disruption of ARF1 spacing interfered with ARF1-dependent trafficking but not with coat protein recruitment. A mutation impairing the interaction of one of the two SEC7 domains of the GNOM ARF-GEF dimer with its ARF1 substrate reduced the efficiency of coordinated ARF1 activation. Our results suggest a model of coordinated activation-dependent membrane insertion of ARF1•GTP molecules required for coated membrane vesicle formation. Considering the evolutionary conservation of ARFs and ARF-GEFs, this initial regulatory step of membrane trafficking might well occur in eukaryotes in general.
Clicker Training Accelerates Learning of Complex Behaviors but Reduces Discriminative Abilities of Yucatan Miniature Pigs

Simple Summary Animal training is intended to teach specific behavioral responses to specific requests. Clicker Training (CT) is a method to train animals based on the use of a device that emits a sound to be associated as a marker that predicts the delivery of something wanted (food). It is believed that CT decreases training time compared to other types of training that use different markers, such as voice. Herein, we used two-month-old miniature piglets to assess whether CT decreased the number of repeats required to learn complex behaviors compared to voice-trained animals. Furthermore, we compared the number of correct choices of animals from both groups when tested for the discrimination of objects. The results indicated that CT decreased the number of repetitions required to learn to fetch an object but reduced the animals’ ability to make correct decisions during discriminatory trials compared to voice-trained animals. This suggests that CT is more efficient than voice in teaching complex behaviors but reduces the ability of animals to use the cognitive processes necessary to discriminate and select objects associated with reward. Animal trainers might consider our results to decide which marker is to be implemented based on the aim and purpose of the training. Abstract Animal training is meant to teach specific behavioral responses to specific cues. Clicker training (CT) is a popular training method based on the use of a device that emits a sound of double-click to be associated as a first-order conditioned stimulus in contingency with positive reinforcements. After some repetitions, the clicker sound gains some incentive value and can be paired with the desired behavior. Animal trainers believed that CT can decrease training time compared to other types of training. Herein, we used two-month old miniature piglets to evaluate whether CT decreased the number of repetitions required to learn complex behaviors as compared with animals trained with voice instead of the clicker. In addition, we compared the number of correct choices of animals from both groups when exposed to object discriminative tests. Results indicated that CT decreased the number of repetitions required for pigs to learn to fetch an object but reduced the ability of animals to make correct choices during the discriminate trials. This suggests that CT is more efficient than voice to teach complex behaviors but reduces the ability of animals to use cognitive processes required to discriminate and select objects associated with reward.
A Reliable Fault Diagnosis Method for a Gearbox System with Varying Rotational Speeds

The vibration signals of gearbox gear fault signatures are informative components that can be used for gearbox fault diagnosis and early fault detection. However, the vibration signals are normally non-linear and non-stationary, and they contain background noise caused by data acquisition systems and the interference of other machine elements. Especially in conditions with varying rotational speeds, the informative components are blended with complex, unwanted components inside the vibration signal. Thus, to use the informative components from a vibration signal for gearbox fault diagnosis, the noise needs to be properly distilled from the informational signal as much as possible before analysis. This paper proposes a novel gearbox fault diagnosis method based on an adaptive noise reducer–based Gaussian reference signal (ANR-GRS) technique that can significantly reduce noise and improve classification from a one-against-one, multiclass support vector machine (OAOMCSVM) for the fault types of a gearbox. The ANR-GRS processes the shaft rotation speed to access and remove noise components in the narrowbands between two consecutive sideband frequencies along the frequency spectrum of a vibration signal, enabling the removal of enormous noise components with minimal distortion to the informative signal. The optimal output signal from the ANR-GRS is then extracted into many signal feature vectors to generate a qualified classification dataset. Finally, the OAOMCSVM classifies the health states of an experimental gearbox using the dataset of extracted features. The signal processing and classification paths are generated using the experimental testbed. The results indicate that the proposed method is reliable for fault diagnosis in a varying rotational speed gearbox system.
Cytoprotective Role of Omentin Against Oxidative Stress-Induced Vascular Endothelial Cells Injury

Endothelial cell injury caused by reactive oxygen species (ROS) plays a critical role in the pathogenesis of cardiovascular diseases. Omentin, an adipocytokine that is abundantly expressed in visceral fat tissue, has been reported to possess anti-inflammatory and antidiabetic properties. However, endothelial protective effects of omentin against oxidative stress remain unclear. This study aimed to evaluate the protective effect of omentin against hydrogen peroxide (H2O2)-induced cell injury in human umbilical vein endothelial cells (HUVECs). Cytotoxicity and cytoprotective effects of omentin were evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptotic activity of HUVECs was detected using Annexin-V/PI and Hoechst 33258 staining methods. Antioxidant activity of omentin was evaluated by measuring both reactive oxygen species (ROS) levels and glutathione peroxidase (GPx) activity. No cytotoxicity effect was observed in HUVECs treated with omentin alone at concentrations of 150 to 450 ng/ml. MTT assay showed that omentin significantly prevented the cell death induced by H2O2 (p < 0.001). Hoechst staining and flow cytometry also revealed that omentin markedly prevented H2O2-induced apoptosis. Moreover, omentin not only significantly inhibited ROS production (p < 0.01) but also significantly (p < 0.01) increased GPx activity in HUVECs. In conclusion, our data suggest that omentin may protect HUVECs from injury induced by H2O2.
Cognitive Training Attenuates Decline in Physical Function across 10 Years.

OBJECTIVES Poor physical function is associated with negative health and cognitive outcomes. Although nine studies demonstrate that cognitive training reduces age-related declines in physical function, only one has examined effects beyond immediate posttest changes. The current study assessed the impact of three cognitive training programs on physical function measures across 10 years. A second aim examined whether baseline cognitive self-efficacy or depressive symptoms moderated training effects. METHODS Using data from the Advanced Cognitive Training for Independent and Vital Elderly (ACTIVE) randomized controlled trial, older adults in a no-contact control condition (n = 698) were compared to those receiving processing speed (n = 702), memory (n = 703), or reasoning (n = 694) training. Intention-to-treat (ITT) and dosage analyses were conducted for grip strength and Turn 360. Participants were followed up to 10 years posttest. RESULTS There were no significant ITT effects of processing speed, memory, or reasoning training assignment to any physical function outcome (p > .05). Dosage models indicated that there were small age-related attenuations effects in Turn 360 decline with more processing speed training (b = -.011, p < .001), memory training (b = -.011, p < .001), and reasoning training (b = -.012, p < .001). There was no significant transfer to grip strength. These training gains were greater for those with more baseline depressive symptoms who received more processing speed training (b = -.001, p < .001). DISCUSSION This is the first study to demonstrate effects of cognitive training to complex physical function across 10 years.
Functional Characterization of POFUT1 Variants Associated with Colorectal Cancer

Background: Protein O-fucosyltransferase 1 (POFUT1) overexpression, which is observed in many cancers such as colorectal cancer (CRC), leads to a NOTCH signaling dysregulation associated with the tumoral process. In rare CRC cases, with no POFUT1 overexpression, seven missense mutations were found in human POFUT1. Methods: Recombinant secreted forms of human WT POFUT1 and its seven mutated counterparts were produced and purified. Their O-fucosyltransferase activities were assayed in vitro using a chemo-enzymatic approach with azido-labeled GDP-fucose as a donor substrate and NOTCH1 EGF-LD26, produced in E. coli periplasm, as a relevant acceptor substrate. Targeted mass spectrometry (MS) was carried out to quantify the O-fucosyltransferase ability of all POFUT1 proteins. Findings: MS analyses showed a significantly higher O-fucosyltransferase activity of six POFUT1 variants (R43H, Y73C, T115A, I343V, D348N, and R364W) compared to WT POFUT1. Interpretation: This study provides insights on the possible involvement of these seven missense mutations in colorectal tumors. The hyperactive forms could lead to an increased O-fucosylation of POFUT1 protein targets such as NOTCH receptors in CRC patients, thereby leading to a NOTCH signaling dysregulation. It is the first demonstration of gain-of-function mutations for this crucial glycosyltransferase, modulating NOTCH activity, as well as that of other potential glycoproteins.
Níveis dietéticos de lisina para frangos de corte de 1 a 21 e 22 a 40 dias de idade

Two experiments were carried out to determine the nutritional requirement of total lysine for Ross broiler chickens from one to 21 and 22 to 40 days of age, of both sexes. The parameters evaluated were weight gain, feed intake, feed conversion and carcass evaluation. In each experiment, a completely randomized experimental design, in a 2 x 6 (two sexes and six dietary lysine levels) factorial arrangement with six replicates, using a total of 1440 birds, was used. The basal diets with 1.03% total lysine, 22.0% CP and 3000 kcal ME/kg in the first experiment and 0.92% total lysine, 20.0% CP and 3150 kcal ME/kg in the second experiment, suplemented with six levels pure lysine (0.0; 0.06; 0.12; 0.18; 0.24 and 0.30%), were used. Considering weigth gain and feed conversion, the dietary requirement estimates for the experiment from 1 to 21 days were 1.303 and 1.249% total lysine and 1.183 and 1.129% digestible lysine for males and females, respectively. For the experiment from 22 to 40 days, the performance and evaluation carcass values were 1.164 and 1.143% total lysine and 1.044 and 1.023% digestible lysine for males and females, respectively.
The expression of the nodD and nodABC genes of Rhizobium leguminosarum is not regulated in response to combined nitrogen

Using a Rhizobium leguminosarum bv. viciae strain harboring nodD :: lacZ or nodC :: lacZ translational fusions, grown in minimal media containing different concentrations of nitrate and/or ammonium salts, lacZ expression was monitored. Based on these experiments it is shown that the induction of Rhizobium leguminosarum bv. viciae nodD and nodABC operons by the flavanone naringenin is not regulated in response to nitrate and/or ammonium salts.
Measurement of aphid feeding rates on artificial diets using 3 H‐inulin

The radioactivity of the honeydew droplets excreted by young apterous adults of the green peach aphid, Myzus persicae, fed on an artificial diet containing 3H‐inulin was a reliable measure of the volume of food ingested by the insects, since almost none of the ingested inulin was absorbed and retained by the insects.
School-based Sexuality Education Experiences across Three Generations of Sexual Minority People

ABSTRACT Sexual minority people face greater risk for compromised sexual health than their heterosexual peers, yet school-based sexuality education often excludes them. Little is known about whether or how sexual minority people’s sexuality education experiences have varied across sociohistorical contexts of rapid social change in both sexuality education and sexual minority visibility. Semi-structured qualitative interviews were conducted among 191 sexual minority people from three distinct sociohistorical generations (ages 18–25, 34–41, and 52–59, respectively) and four geographic regions of the United States. Data were analyzed using thematic content analysis following a consensual qualitative protocol. Fifty-one participants (i.e., 27%) discussed school-based sexuality education experiences despite the lack of an explicit question in the interview protocol prompting them to do so. Four distinct yet overlapping themes emerged in participants’ experiences of sexuality education: 1) Silence; 2) The profound influence of HIV/AIDS; 3) Stigma manifest through fear, shame, and prejudice; and, 4) Comparing school-based experiences to sexuality education outside of school. The presence of themes varied across groups defined by sociohistorical generation. The implications of sexuality education experiences for the wellbeing of sexual minority people are discussed.
VLSI Structures for DNA Sequencing—A Survey

DNA sequencing is a critical functionality in biomedical research, and technical advances that improve it have important implications for human health. Novel methods by which sequencing can be accomplished in more accurate, high-throughput, and faster ways are in development. Here, we review VLSI biosensors for nucleotide detection and DNA sequencing. Implementation strategies are discussed and split into function-specific architectures that are presented for reported design examples from the literature. Lastly, we briefly introduce a new approach to sequencing using Gate All-Around (GAA) nanowire Metal Oxide Semiconductor Field Effect Transistors (MOSFETs) that has significant implications for the field.
Efficacy and Safety of Pulsed Radiofrequency as a Method of Dorsal Root Ganglia Stimulation in Patients with Neuropathic Pain: A Systematic Review.

OBJECTIVE Pulsed radiofrequency (PRF) is a nonablative pain treatment that uses radiofrequency current in short high-voltage bursts, resulting in interruption of nociceptive afferent pathways. We conducted a systematic review with the aim to create a synthesis of evidence about the efficacy and safety of PRF applied to the dorsal root ganglion (DRG) for the treatment of neuropathic pain. METHODS We searched MEDLINE, CINAHL, Embase, and PsycINFO through January 8, 2019, as well as ClinicalTrials.gov and the clinical trial register of the World Health Organization. All study designs were eligible. We assessed risk of bias using the Cochrane tool for randomized controlled trials and the Risk Of Bias In Non-Randomized Studies of Interventions (ROBINS-I). We assessed level of evidence using the Oxford tool and quality of evidence with GRADE. RESULTS We included 28 studies with participants suffering from lumbosacral, cervical, or thoracic radicular pain, post-herpetic neuralgia, neuropathicbone pain in cancer patients, or carpal tunnel syndrome. Only five studies were randomized controlled trials (RCTs), while others were of nonrandomized designs, predominantly before and after comparisons. A total of 991 participants were included, with a median number (range) of 31 (1-101) participants. Only 204 participants were included in the RCTs, with a median number (range) of 38 (23-62) participants. The overall quality of evidence was low, as the majority of the included studies were rated as evidence level 4 or 5. The quality of evidence was very low. CONCLUSIONS Evidence about the efficacy and safety of PRF of the DRG for the treatment of neuropathic pain is based mainly on results from very small studies with low evidence quality. Current research results about the benefits of PRF of the DRG for the treatment of neuropathic pain should be considered preliminary and confirmed in high-quality RCTs with sufficient numbers of participants.