Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of a Mutated DNA Ligase IV Gene in the X-ray-hypersensitive Mutant SX10 of Mouse FM3A Cells*

The mouse carcinoma cell line SX10 is a hypersensitive mutant to x-rays and bleomycin. An earlier complementation test suggests that SX10 would belong to x-ray-cross complementing group (XRCC) 4. However, in this study, a human XRCC4 expression vector failed to complement the SX10 phenotype. Consistent with the previous report, SX10 showed the same level of DNA-dependent protein kinase activity as the wild-type SR-1. We isolated and analyzed hybrids between SX10 and human diploid fibroblast cells and found that human chromosome 13 conferred the x-ray resistance to the hybrids, suggesting that a candidate gene would be located on this chromosome. Polymerase chain reaction analysis with these hybrids and x-ray-resistant transformants obtained by introducing human chromosomes into SX10 indicated that the mutant was likely to be defective in DNA ligase IV. Sequence analysis of the DNA ligase IV gene confirmed that a defect in SX10 was attributed to a transition of G to A at nucleotide position 1413 of the gene, leading to an amino acid substitution from Trp at residue 471 to a stop codon. Revertant clones (Rev1–3) derived from SX10 showed a restored x-ray resistance; Rev1 reverted to the original nucleotide G at position 1413, whereas Rev2 and Rev3 to C. Transfection of a mouse DNA ligase IV cDNA vector into SX10 restored the resistance to both x-rays and bleomycin. SX10 showed a reduced frequency of chromosomal integration of transfected DNA, but the revertants restored the frequency found in the wild-type cells. These results suggest a possible involvement of DNA ligase IV in the integration event of foreign DNA as well as a crucial role in DNA double-strand break repair.
Ground-based Tracking of Geosynchronous Space Objects with a GM-CPHD Filter

This paper presents a multi-target tracker for space objects near geosynchronous orbit using the Gaussian Mixture Cardinalized Probability Hypothesis Density (CPHD) filter. Given limited sensor coverage and more than 1,000 objects near geosynchronous orbit, long times between measurement updates for a single object can yield propagated uncertainties sufficiently large to create ambiguities in observation-to-track association. Recent research considers various methods for tracking space objects via Bayesian multi-target filters, with the CPHD being one such example. The implementation of the CPHD filter presented in this paper includes models consistent with the space-object tracking problem to form a new space-object tracker. This tracker combines parallelization with efficient models and integrators to reduce the run time of Gaussian-component propagation. To allow for instantiating new objects, the proposed filter uses a variation of the probabilistic admissible region that adheres to assumptions in the derivation of the CPHD filter. Finally, to reduce computation time while mitigating the so-called “spooky action at a distance” phenomenon in the CPHD filter, we propose splitting the multi-target state into distinct, non-interacting populations based on the sensor’s field of view. In a scenario with 700 near-geosynchronous objects observed via three ground stations, the tracker maintains custody of initially known objects and instantiates tracks for newly detected ones. The mean filter estimation after a 48 hour observation campaign is comparable to the measurement error statistics.
Energy-aware user association and energy cooperation for smart-grid-powered HetNet

As renewable energy is intermittent and randomly distributed, it is difficult for energy harvesting enabled networks to reliably supply the time-varying mobile traffic. We propose a brand new approach of energy sharing and load shifting to overcome this issue. In this paper, we first investigate the user association (UA) problem with energy cooperation to minimize the grid energy expenditure of heterogeneous networks (HetNets) whose base stations (BSs) are powered by both grid and renewable energy. Then we use greedy decomposition to convert this problem into two sub-problems which are energy cost aware user association and energy cooperation. For the first problem, we implement an optimal offline algorithm by using relaxing, dual decomposition. To solve the second problem, we propose a many-to-many matching algorithm. Finally, our simulation results show the effectiveness of the proposed algorithms and demonstrate that our algorithm is the most on-grid energy saving algorithm compared with existing schemes.
Steady-state model and power flow analysis of grid-connected photovoltaic power system

The power flow analysis of a power grid containing photovoltaic (PV) generating system is the foundation of studying steady-state characteristics of large- scale PV power station integrating into power grid. Generally, PV systems are coupled by power electronic converters. Thus, the steady-state modeling can be based on PV array characteristics and principles of power electronic transforms. The model in this paper can simulate steady-state operations of PV systems in condition of giving meteorological, PV system and power grid parameters. Moreover, the alternative and iterative method is used to obtain the unified solution of the model and power flow equations. Being compared with the actual PV station, analytical results indicate that the simulating error during noon time is very small and errors during morning and evening are relatively great. Also, variations of meteorological and power grid parameters will effect the system operation, but the irradiance is the greater one and the grid voltage variation has little effect. The power flow analysis is verified on IEEE 30-bus system, and PV power station can act as not only a PQ node, but a PV node which can supply reactive power to support voltage profiles.
IPSECvalidate { A Tool to Validate IPSEC Con gurations IPSECvalidate { A Tool to Validate IPSEC Con gurations

This paper describes a tool for validating the proper con guration of the IPSEC protocol suite including IKE. The tool validates that two hosts are able to communicate (normal ping functionality) and that this communication is occurring using the proper authentication/encryption transformations as required by IPSEC. IPSEC con guration is very complex, and administrators are often unable to determine if a machine con guration is o ering the desired protection. IPSEC and IKE operate in a manner transparent to IP applications; an administrator is therefore unable to check the proper operation of an IPSEC "security association" using traditional IP tools.
Supplementary Material ECO : Efficient Convolution Operators for Tracking

This supplementary document of [1] first provides the computational complexity analysis of the learning step in C-COT. Section 2 provides additional details of the numerical optimization procedure used for learning the factorized convolution operator (section 3.1 in the paper). We report relevant hardware specifications in section 3. Lastly, we provide additional results on the VOT2016 and OTB-2015 benchmarks in section 4 and 5, respectively.
Cross-resistance between bacteriophages and colicins in Escherichia coli K-12

Cross-resistance between bacteriophages and colicins was studied using collections of bacteriophage- and colicin-resistant mutants of Escherichia coli K-12. No new examples were found of highly specific one-to-one cross-resistance of the type suggestive of common receptors. However, several groups of mutants showed tolerance to colicins and resistance to bacteriophages. Mutants known to be very defective in lipopolysaccharides composition were found to commonly show tolerance to certain colicins in addition to their bacteriophage resistance. Another group of mutants showed varying patterns of resistance to colicins E2, E3, K, L, A, S4, N, and X and bacteriophages E4, K2, K20, K21, K29, and H+. However, many bacteriophage-resistant mutants were fully colicin sensitive, and most colicin-resistant mutants were fully sensitive to bacteriophages.
Entrapment of Bacteriocin 105B onto Fabric with Titania

Abstract : Current methods to fabricate multifunctional textiles to provide multiple facets of protection to the Warfighter are restrictive and not efficient. To address this challenge, previous work by the FORCE ProTex effort encapsulated a narrow-spectrum antimicrobial, the bacteriocin nisin, in a photocatalytic matrix, titanium dioxide (titania), on fabric to evaluate the creation of a multifunctional textile in unison. This report summarizes preliminary investigations to leverage this work by employing a different bacteriocin, an isolate from Bacillus anthracis termed 105b, to fabricate a multifunctional textile exhibiting an alternative range of antimicrobial activity from that of nisin, by titania encapsulation of 105b onto fabric. The results of these initial studies suggest that both pure preparations and semi-pure preparations of 105b are active when encapsulated in titania in solution. However, when the pure preparation of 105b is titania encapsulated on fabric, antimicrobial activity is not observed. It is hypothesized that the lack of activity of encapsulated pure 105b may be due to the removal of molecular stabilizers during the purification process that are responsible for maintaining the stability of the peptide, rendering it inactive. Further studies are necessary to confirm this mechanism. Additional work is also required to retain the activity of encapsulated pure 105b, ascertain the photocatalytic activity of the titania matrix, as well as elucidate the conditions for gradual release of the bacteriocin.
Standardized Comparison of the Relative Impacts of HIV-1 Reverse Transcriptase (RT) Mutations on Nucleoside RT Inhibitor Susceptibility

ABSTRACT Determining the phenotypic impacts of reverse transcriptase (RT) mutations on individual nucleoside RT inhibitors (NRTIs) has remained a statistical challenge because clinical NRTI-resistant HIV-1 isolates usually contain multiple mutations, often in complex patterns, complicating the task of determining the relative contribution of each mutation to HIV drug resistance. Furthermore, the NRTIs have highly variable dynamic susceptibility ranges, making it difficult to determine the relative effect of an RT mutation on susceptibility to different NRTIs. In this study, we analyzed 1,273 genotyped HIV-1 isolates for which phenotypic results were obtained using the PhenoSense assay (Monogram, South San Francisco, CA). We used a parsimonious feature selection algorithm, LASSO, to assess the possible contributions of 177 mutations that occurred in 10 or more isolates in our data set. We then used least-squares regression to quantify the impact of each LASSO-selected mutation on each NRTI. Our study provides a comprehensive view of the most common NRTI resistance mutations. Because our results were standardized, the study provides the first analysis that quantifies the relative phenotypic effects of NRTI resistance mutations on each of the NRTIs. In addition, the study contains new findings on the relative impacts of thymidine analog mutations (TAMs) on susceptibility to abacavir and tenofovir; the impacts of several known but incompletely characterized mutations, including E40F, V75T, Y115F, and K219R; and a tentative role in reduced NRTI susceptibility for K64H, a novel NRTI resistance mutation.
EVALUATION OF ANTIDIABETIC, ANTIDYSLIPIDEMIC & HEPATOPROTECTIVE ACTIVITY OF HOMALIUM ZEYLANICUM IN ALLOXAN INDUCED DIABETIC RATS

To evaluate the antidiabetic activity of Homalium zeylanicum stem bark extracts against Alloxan induced Diabetes mellitus. Materials and methods: Diabetes was induced in male Wistar rats by intraperitoneal injection of Alloxan (90mg/kg). Ethanol extract of Homalium zeylanicum stem bark were administered to the experimental rats (250mg and 500mg/kg, p.o. for 28 days). The antidiabetic & antidyslipidemic, effects of these extracts was evaluated by the assay of biochemical parameters (Blood glucose and lipid profiles, SGOT, SGPT) and histopathological studies of the liver. Results: In Ethanol extract-treated animals, the hyperglycemia by the Alloxan was controlled significantly by restoration of the levels of serum glucose, lipid profile and liver enzymes as compared to the normal and the standard drug Metformin treated groups. Histology of the liver sections of the animals treated with the extracts showed the presence of normal hepatics, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity. Conclusion: Ethanol extract of the stem bark of Homalium
Contributions of Perceptual and Motor Experience of an Observed Action to Anticipating Its Result

To gain deeper insight into respective contributions of perceptual and motor experience of an observed action to anticipating its result, we examined the perceptual anticipation of players with different action roles in striking sports. Baseball pitchers and batters at both advanced and intermediate levels were asked to make a decision about whether to swing the bat when viewing a series of videos showing incomplete sequences of a model pitcher throwing a strike or a ball. The results revealed that first 100 ms of ball flight could discriminate advanced batters from intermediate pitchers and batters (with no difference between intermediate pitchers and batters). Particularly, advanced batters (perceptual experts with regard to pitching action) were statistically more accurate and less uncertain in making decisions than were intermediate players, whereas advanced pitchers (motor experts) only showed this tendency without reaching a statistically significant level. Moreover, advanced batters demonstrated greater perceptual sensitivity in discriminating when to swing at strikes over balls than all other players. Our findings suggested that when players were above intermediate level, perceptual experience of an observed action facilitated the perceptual anticipation to a greater extent than motor experience of producing it.
EXTRACTING SECONDARY BIO‐EVENT ARGUMENTS WITH EXTRACTION CONSTRAINTS

This paper describes our bio‐event extraction system developed for the BioNLP 2009 Shared Task 2, with focus on its capability of extracting secondary biological event arguments from literature. Shared Task 2 is particularly interesting because when browsing literature, biologists often need to understand conditions surrounding biological events, which are usually expressed by secondary event arguments (e.g., binding sites). To achieve our goal, we take an approach that extracts n‐ary relations from text using event extraction constraints automatically generated from a training corpus. Event constraints consist of sequences of trigger words and semantic roles which we automatically identify using Conditional Random Fields (CRFs). Unlike most other systems participating in this shared task, our system is light‐weight and relies on neither external resources (e.g., Ontologies and dictionaries) nor natural language processing software (e.g., POS taggers and parsers). The official test results show that our approach performed well on extracting secondary arguments in Task 2, yielding the highest precision at 76.62% and the second highest F‐measure at 43.22%.
Rice husk-derived graphene with nano-sized domains and clean edges.

A new synthetic method is demonstrated for transforming rice husks into bulk amounts of graphene through its calcination and chemical activation. The bulk sample consists of crystalline nano-sized graphene and corrugated individual graphene sheets; the material generally contains one, two, or a few layers, and corrugated graphene domains are typically observed in monolayers containing topological defects within the hexagonal lattice and edges. Both types of graphenes exhibit atomically smooth surfaces and edges.
Detection of alloantibodies using a sensitive antiglobulin microcytotoxicity test: identification of low levels of pre-formed antibodies in accelerated allograft rejection.

An antiglobulin test, more sensitive than established direct microcytotoxicity tests, was developed to detect both complement fixing and non-complement fixing alloantibodies. The test has been used to detect antibodies in sera from selected organ allograft recipients. Of 56 sera tested by the antiglobulin method, 52 (93%) were reactive. In contrast, only 13 (23%) were reactive in an established direct microcytotoxicity test and only 28 (50%) were reactive in a modification of the direct test in which the incubation times were doubled. Sera obtained prior to transplantation from 3 patients who experienced either immediate or early rejection of their renal allografts contained antibodies detected by the antiglobulin test but not by direct microcytotoxicity tests against lymphocytes from a panel of unrelated, normal individuals. In addition, the antiglobulin test has been used to demonstrate that the humoral antibody response following transplantation of cardiac and renal allografts is heterogeneous with respect to formation of antibodies of the IgA, IgG and IgM classes.
Modelling thermal phenomena in semiconductor devices and magnetic elements of boost converter using averaged models

This paper is devoted to analyses of the boost converter using electrothermal average models. The modified forms of electrothermal average models of the diode - transistor switch and the inductor dedicated for SPICE are shown. The value of parameters of the considered models are estimated on the basis of the catalogue data for the considered converter components. The computations were performed for boost converter including inductors with cores made of powdered iron or ferrite. The obtained results of computations of the electrothermal average model are compared with the results of measurements and computations made by the selected literature models.
Nuclear and mitochondrial markers reveal distinctiveness of a small population of bottlenose whales (Hyperoodon ampullatus) in the western North Atlantic

Small populations at the edge of a species’ distribution can represent evolutionary relics left behind after range contractions due to climate change or human exploitation. The distinctiveness and genetic diversity of a small population of bottlenose whales in the Gully, a submarine canyon off Nova Scotia, was quantified by comparison to other North Atlantic populations using 10 microsatellites and mitrochondrial DNA (mtDNA) control region sequences (434 bp). Both markers confirmed the distinctiveness of the Gully (n = 34) from the next nearest population, off Labrador (n = 127; microsatellites –FST= 0.0243, P < 0.0001; mtDNA –ΦST = 0.0456, P < 0.05). Maximum likelihood microsatellite estimates suggest that less than two individuals per generation move between these areas, refuting the hypothesis of population links through seasonal migration. Both males and females appear to be philopatric, based on significant differentiation at both genomes and similar levels of structuring among the sexes for microsatellites. mtDNA diversity was very low in all populations (h = 0.51, π = 0.14%), a pattern which may be due to selective sweeps associated with this species’ extreme deep‐diving ecology. Whaling had a substantial impact on bottlenose whale abundance, with over 65 000 animals killed before the hunt ceased in the early 1970s. Genetic diversity was similar among all populations, however, and no signal for bottlenecks was detected, suggesting that the Gully is not a relic of a historically wider distribution. Instead, this unique ecosystem appears to have long provided a stable year‐round habitat for a distinct population of bottlenose whales.
The Arabidopsis Circadian Clock Incorporates a cADPR-Based Feedback Loop

Transcriptional feedback loops are a feature of circadian clocks in both animals and plants. We show that the plant circadian clock also incorporates the cytosolic signaling molecule cyclic adenosine diphosphate ribose (cADPR). cADPR modulates the circadian oscillator's transcriptional feedback loops and drives circadian oscillations of Ca2+ release. The effects of antagonists of cADPR signaling, manipulation of cADPR synthesis, and mathematical simulation of the interaction of cADPR with the circadian clock indicate that cADPR forms a feedback loop within the plant circadian clock.
Carleman estimates for the Zaremba Boundary Condition and Stabilization of Waves

In this paper, we shall prove a Carleman estimate for the so-called Zaremba problem. Using some techniques of interpolation and spectral estimates, we deduce a result of stabilization for the wave equation by means of a linear Neumann feedback on the boundary. This extends previous results from the literature: indeed, our logarithmic decay result is obtained while the part where the feedback is applied contacts the boundary zone driven by an homogeneous Dirichlet condition. We also derive a controllability result for the heat equation with the Zaremba boundary condition.
4 Framing Wicked Problems in Enterprise-Systems Innovation Project Groups

Organizations are increasingly focusing on the design of organizational information systems that incorporate both business process and information technology (IT) change, to achieve alignment between business and IT objectives. These information systems (IS) span functional boundaries and business units, requiring many different disciplinary, organizational, and political interests to be negotiated and multiple, diverse ways of working to be reconciled. Organizational IS design, especially when this spans functional or business unit boundaries, conceptualizes and implements changes to the organization and practices of work, as well as technological change. IS managers thus need to balance the need for actionable, objective defi nitions of IT-related change with a need for radical, boundary-spanning inquiry into what needs to change (Engeström, Engeström, and Karkkainen 1995; Liu, Sun, and Bennett 2002). In the organizational and MIS literature, we have an increasing awareness of the emergent nature of IT-systems defi nition and its embeddedness within wider processes of organizational problem solving and business-process change. The term information system is used here to mean an integrated social system of organizational actors, using information to perform purposeful activity, who may or may not use computerbased technology to facilitate their work and to provide information (Silva and Hirschheim 2007). Following the use of the term in other fi elds, such as architecture, product design, or graphic design, the term design is viewed here as the complete process of conceptualizing, developing, refi ning, and evaluating an artifact-related solution to a problem, rather than as a single stage of the IT systems development life cycle (Lawson, 2000). Increasingly, the critical processes of boundary-spanning design take place “upstream” of the development life cycle waterfall model. Organizational change and problem-solving projects drive business process change and provide “early requirements” for IT systems development. This form of design involves emergent knowledge processes, for which goals emerge during the process of design (Markus et al., 2002). As I will argue below, it involves a dialectic between the processes of inquiry and processes of closure, whereas the typical IS design method focuses exclusively on closure.