Miracle-dz/UniFAR-FTUs huggingface dataset:
Involvement of Innate Immune Receptors in the Resolution of Acute Hepatitis B in Woodchucks

The antiviral property of small agonist compounds activating pattern recognition receptors (PRRs), including toll-like and RIG-I receptors, have been preclinically evaluated and are currently tested in clinical trials against chronic hepatitis B (CHB). The involvement of other PRRs in modulating hepatitis B virus infection is less known. Thus, woodchucks with resolving acute hepatitis B (AHB) after infection with woodchuck hepatitis virus (WHV) were characterized as animals with normal or delayed resolution based on their kinetics of viremia and antigenemia, and the presence and expression of various PRRs were determined in both outcomes. While PRR expression was unchanged immediately after infection, most receptors were strongly upregulated during resolution in liver but not in blood. Besides well-known PRRs, including TLR7/8/9 and RIG-I, other less-characterized receptors, such as IFI16, ZBP1/DAI, AIM2, and NLRP3, displayed comparable or even higher expression. Compared to normal resolution, a 3–4-week lag in peak receptor expression and WHV-specific B- and T-cell responses were noted during delayed resolution. This suggested that PRR upregulation in woodchuck liver occurs when the mounting WHV replication reaches a certain level, and that multiple receptors are involved in the subsequent induction of antiviral immune responses. Liver enzyme elevations occurred early during normal resolution, indicating a faster induction of cytolytic mechanisms than in delayed resolution, and correlated with an increased expression of NK-cell and CD8 markers and cytolytic effector molecules. The peak liver enzyme level, however, was lower during delayed resolution, but hepatic inflammation was more pronounced and associated with a higher expression of cytolytic markers. Further comparison of PRR expression revealed that most receptors were significantly reduced in woodchucks with established and progressing CHB, and several RNA sensors more so than DNA sensors. This correlated with a lower expression of receptor adaptor and effector molecules, suggesting that persistent, high-level WHV replication interferes with PRR activation and is associated with a diminished antiviral immunity based on the reduced expression of immune cell markers, and absent WHV-specific B- and T-cell responses. Overall, the differential expression of PRRs during resolution and persistence of WHV infection emphasizes their importance in the ultimate viral control during AHB that is impaired during CHB.
A Comparative Overview of Epigenomic Profiling Methods

In the past decade, assays that profile different aspects of the epigenome have grown exponentially in number and variation. However, standard guidelines for researchers to choose between available tools depending on their needs are lacking. Here, we introduce a comprehensive collection of the most commonly used bulk and single-cell epigenomic assays and compare and contrast their strengths and weaknesses. We summarize some of the most important technical and experimental parameters that should be considered for making an appropriate decision when designing epigenomic experiments.
Nitric Oxide Functions as a Downstream Signal for Melatonin-Induced Cold Tolerance in Cucumber Seedlings

Melatonin (MT) and nitric oxide (NO) are two multifunctional signaling molecules that are involved in the response of plants to abiotic stresses. However, how MT and NO synergize in response to cold stress affecting plants is still not clear. In this study, we found that endogenous MT accumulation under cold stress was positively correlated with cold tolerance in different varieties of cucumber seedlings. The data presented here also provide evidence that endogenous NO is involved in the response to cold stress. About 100 μM MT significantly increased the nitrate reductase (NR) activity, NR-relative messenger RNA (mRNA) expression, and endogenous NO accumulation in cucumber seedlings. However, 75 μM sodium nitroprusside (SNP, a NO donor) showed no significant effect on the relative mRNA expression of tryptophan decarboxylase (TDC), tryptamine-5-hydroxylase (T5H), serotonin-N-acetyltransferase (SNAT), or acetylserotonin O-methyltransferase (ASMT), the key genes for MT synthesis and endogenous MT levels. Compared with H2O treatment, both MT and SNP decreased electrolyte leakage (EL), malondialdehyde (MDA), and reactive oxygen species (ROS) accumulation by activating the antioxidant system and consequently mitigated cold damage in cucumber seedlings. MT and SNP also enhanced photosynthetic carbon assimilation, which was mainly attributed to an increase in the activity and mRNA expression of the key enzymes in the Calvin–Benson cycle. Simultaneously, MT- and SNP-induced photoprotection for both photosystem II (PSII) and photosystem I (PSI) in cucumber seedlings, by stimulating the PsbA (D1) protein repair pathway and ferredoxin-mediated NADP+ photoreduction, respectively. Moreover, exogenous MT and SNP markedly upregulated the expression of chilling response genes, such as inducer of CBF expression (ICE1), C-repeat-binding factor (CBF1), and cold-responsive (COR47). MT-induced cold tolerance was suppressed by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, a specific scavenger of NO). However, p-chlorophenylalanine (p-CPA, a MT synthesis inhibitor) did not affect NO-induced cold tolerance. Thus, novel results suggest that NO acts as a downstream signal in the MT-induced plant tolerance to cold stress.
Parent and Teacher-Reported Child Outcomes Seven Years After Mild Traumatic Brain Injury: A Nested Case Control Study

Background: Increasing evidence suggests potential lifetime effects following mild traumatic brain injury (TBI) in childhood. Few studies have examined medium-term outcomes among hospitalized and non-hospitalized samples. Study aims were to describe children's behavioral and emotional adjustment, executive function (EF), quality of life, and participation at 7-years following mild TBI using parents' and teachers' reports. Methods: Nested case control study of 86 children (68% male, mean age at assessment = 11.27 years; range 7–17 years) who sustained a mild TBI 7-years previously, identified from a prospective, population-based study. They were compared to 69 children free from TBI (61% male, mean age at assessment = 11.12 years; range 5–17 years). In addition to parent-reported socio-demographic details, parents (mild TBI n = 86, non-TBI n = 69) completed age-appropriate standardized questionnaires about children's health-related quality of life, behavioral and emotional adjustment, EF, and social participation. Parents own mood was assessed using the Hospital Anxiety and Depression Scale. Teachers (mild TBI n = 53, non-TBI n = 42) completed questionnaires about children's behavioral and emotional adjustment, and EF. Results: Parent reports showed median group-level scores for cases were statistically significantly greater than controls for emotional symptoms, conduct problems, hyperactivity/inattention, total behavioral difficulties, inhibitory control, shifting, planning/organizing, and Global Executive Composite (total) EF difficulties (p-values 0.001–0.029). Parent reports of child quality of life and social participation were similar, as were teacher reports of child behavioral and emotional adjustment, and EF (p > 0.05). When examining clinical cut-offs, compared to controls, cases had a higher risk of parent-reported total EF difficulties (odds ratio = 3.00) and, to a lesser extent, total behavior problems (odds ratio = 2.51). Conclusions: As a group, children with a history of mild TBI may be at elevated risk for clinically significant everyday EF difficulties in the medium-term compared to non-TBI controls, as judged by their parents. Further multi-informant longitudinal research is required, following larger samples. Aspects requiring particular attention include pre-injury characteristics, such as sleep disturbances and comorbidities (e.g., headaches), that may act as potential confounders influencing the association between mild TBI and child behavioral problems.
“β-Cyclodextrin nano-reactor”-catalyzed synthesis of 2H-chromene-2,3-dicarboxylates from in-situ-generated stabilized phosphorus ylides via intramolecular Wittig reaction in water

GRAPHICAL ABSTRACT Abstract A convenient one-pot synthesis of chromene derivatives from the three-component reaction of triphenylphosphine and dialkyl acetylenedicarboxylate and 2-hydroxybenzaldehyde derivatives in the presence of ß-cyclodextrin as a nano-catalyst is reported. In this reaction, ß-cyclodextrin (4.5 mole%) acts as a nano-catalyst as well as nano-reactor. This methodology is of interest due to the use of water in the presence of “ß-cyclodextrin nano-reactor” as a green reaction system.
The Influence of Combined Turbulators on the Hydraulic-Thermal Performance and Exergy Efficiency of MWCNT-Cu/Water Nanofluid in a Parabolic Solar Collector: A Numerical Approach

The present research benefits from the finite volume method in investigating the influence of combined turbulators on the thermal and hydraulic exergy of a parabolic solar collector with two-phase hybrid MWCNT-Cu/water nanofluid. All parabolic geometries are produced using DesignModeler software. Furthermore, FLUENT software, equipped with a SIMPLER algorithm, is applied for analyzing the performance of thermal and hydraulic, and exergy efficiency. The Eulerian–Eulerian multiphase model and k-ε were opted for simulating the two-phase hybrid MWCNT-Cu/water nanofluid and turbulence model in the collector. The research was analyzed in torsion ratios from 1 to 4, Re numbers from 6,000 to 18,000 (turbulent flow), and the nanofluid volume fraction of 3%. The numerical outcomes confirm that the heat transfer and lowest pressure drop are relevant to the Re number of 18,000, nanofluid volume fraction of 3%, and torsion ratio of 4. Furthermore, in all torsion ratios, rising Re numbers and volume fraction lead to more exergy efficiency. The maximum value of 26.32% in the exergy efficiency was obtained at a volume fraction of 3% and a torsion ratio of 3, as the Re number goes from 60,000 to 18,000.
Tool-assisted Threat Modeling for Smart Grid Cyber Security

Threat modeling is about identifying architectural flaws and weaknesses in a system in order to mitigate them and avoid unwanted incidents caused by an attacker. Tool-assisted threat modeling has seen limited use in complex cyber-physical systems involving both Information Technology (IT) and Operational Technology (OT) systems. In this paper, we investigate the applicability of tool-assisted threat modeling to the complex cyber-physical system that is the smart grid, and present a new Smart Grid template for the Microsoft Threat Modeling Tool. We demonstrate benefits of our smart grid threat modeling template on a use-case, and discuss limitations.
Rural Tourism and the Sustainable Development Goals. A Study of the Variables That Most Influence the Behavior of the Tourist

Tourism is an activity that contributes directly and indirectly to the development of rural areas. But this development needs to be sustainable. To do this, appropriate policies that positively influence these areas from an economic, social and cultural point of view must be implemented. All this in accordance with the Sustainable Development Goals. This study will analyze the contribution of rural tourism to develop and implement policies to promote sustainable tourism that creates jobs and promotes local culture and products. The variables that most influence the tourist behavior, motivation, the destination image, and the satisfaction obtained by the tourist will be analyzed. After an exhaustive review of the literature, an empirical investigation was carried out with 1,658 valid surveys among rural tourists in Soria, a Spanish province with one of the highest levels of depopulation. A structural equation model was drawn up to discover the relationships between the variables. The results demonstrated the importance of the motivation in the formation of the destination image, as well as satisfaction with the trip. In the same way, we will verify which component of the image of the destination (affective or cognitive) has the most influence on their formation, and how the image of the destination, like motivation, influences tourist satisfaction. The proposed model could be used in many studies that analyze the different variables that influence consumer behavior since its reliability and predictive capacity have been proven. The results of the study can also be used by the authorities to design or modify the most appropriate strategies that influence rural tourism, specially promoting the destination image as a variable that positively influences tourist satisfaction.
Specific White Matter Tracts and Diffusion Properties Predict Conversion From Mild Cognitive Impairment to Alzheimer’s Disease

Identifying biomarkers that can assess the risk of developing Alzheimer’s Disease (AD) remains a significant challenge. In this study, we investigated the integrity levels of brain white matter in 34 patients with mild cognitive impairment (MCI) who later converted to AD and 53 stable MCI patients. We used diffusion tensor imaging (DTI) and automated fiber quantification to obtain the diffusion properties of 20 major white matter tracts. To identify which tracts and diffusion measures are most relevant to AD conversion, we used support vector machines (SVMs) to classify the AD conversion and non-conversion MCI patients based on the diffusion properties of each tract individually. We found that diffusivity measures from seven white matter tracts were predictive of AD conversion with axial diffusivity being the most predictive diffusion measure. Additional analyses revealed that white matter changes in the central and parahippocampal terminal regions of the right cingulate hippocampal bundle, central regions of the right inferior frontal occipital fasciculus, and posterior and anterior regions of the left inferior longitudinal fasciculus were the best predictors of conversion from MCI to AD. An SVM based on these white matter tract regions achieved an accuracy of 0.75. These findings provide additional potential biomarkers of AD risk in MCI patients.
Global Overview of Youth Development: Comparison of the 5 Cs and Developmental Assets Across Six Countries

Positive Youth Development (PYD) frameworks which describe young people's strengths and their relation to thriving and risk outcomes have gained significance among developmental researchers globally. As these models are being increasingly adopted, concerns remain about their generalizability outside of North America. It has been observed that the distribution and salience of assets differ for young people based on their cultural context. To better understand these varying developmental patterns, this paper studies the distribution of developmental assets and 5 Cs (Competence, Confidence, Character, Caring, and Connection) in youth from various countries and contrasting backgrounds. The total sample consisted of 4,175 students (62.5% females) with age ranging from 15 to 25 years (M = 18.95, SD = 2.49). 981 students were from Ghana (52.5% females), 900 students from Kosovo (66.7% females), 425 students from Norway (73.5% females), 247 students from Portugal (42.1% females), 648 students from Slovenia (63.4% females,), and 974 students from Turkey (68.7% females). Before comparisons of the countries, partial scalar invariance was confirmed. Analyses revealed that all countries differed in at least some internal or external developmental assets and at least in one of the 5 Cs. When considering internal assets, participants from Ghana seemed to have higher levels of internal assets together with participants from Norway who have the highest commitment to learning. Slovenian youth reported the highest levels of external assets of support and empowerment. Regarding the 5 Cs, Ghanaian youth reported having the highest confidence and character, and youth from Ghana, Kosovo, and Turkey are more caring and connected to others. The results uncovered unique patterns of PYD for each included country which are discussed through the lens of its political and social context. Through this focus on cross-national PYD patterns, this study advanced knowledge about the experiences of youth from a wide range of backgrounds and put forth suggestions for better policy measures and more culturally relevant interventions for optimal development of youth embedded in different cultures and countries.
Microvolt T-Wave Alternans Is Modulated by Acute Low-Level Tragus Stimulation in Patients With Ischemic Cardiomyopathy and Heart Failure

Aims: Microvolt T-wave alternans (TWA), an oscillation in T-wave morphology of the electrocardiogram (ECG), has been associated with increased susceptibility to ventricular tachy-arrhythmias, while vagus nerve stimulation has shown promising anti-arrhythmic effects in in vivo and ex vivo animal studies. We aimed to examine the effect of non-invasive, acute low-level tragus stimulation (LLTS) on TWA in patients with ischemic cardiomyopathy and heart failure. Methods: 26 patients with ischemic cardiomyopathy (left ventricular ejection fraction <35%) and chronic stable heart failure, previously implanted with an automatic implantable cardioverter defibrillator (ICD) device with an atrial lead (dual chamber ICD or cardiac resynchronization therapy defibrillator), were enrolled in the study. Each patient sequentially received, (1) Sham LLTS (electrode on tragus, but no stimulation delivered) for 5 min; (2) Active LLTS at two different frequencies (5 and 20 Hz, 15 min each); and (3) Active LLTS, during concomitant atrial pacing at 100 bpm at two different frequencies (5 and 20 Hz, 15 min each). LLTS was delivered through a transcutaneous electrical nerve stimulation device (pulse width 200 μs, frequency 5/20 Hz, amplitude 1 mA lower than the discomfort threshold). TWA burden was assessed using continuous ECG monitoring during sham and active LLTS in sinus rhythm, as well as during atrial pacing. Results: Right atrial pacing at 100 bpm led to significantly heightened TWA burden compared to sinus rhythm, with or without LLTS. Acute LLTS at both 5 and 20 Hz, during sinus rhythm led to a significant rise in TWA burden in the precordial leads (p < 0.05). Conclusion: Acute LLTS results in a heart-rate dependent increase in TWA burden.
Comparison of the Mechanical Properties Between the Convex and Concave Inner/Apical Surfaces of the Developing Cerebrum

The inner/apical surface of the embryonic brain wall is important as a major site for cell production by neural progenitor cells (NPCs). We compared the mechanical properties of the apical surfaces of two neighboring but morphologically distinct cerebral wall regions in mice from embryonic day (E) E12–E14. Through indentation measurement using atomic force microscopy (AFM), we first found that Young’s modulus was higher at a concave-shaped apical surface of the pallium than at a convex-shaped apical surface of the ganglionic eminence (GE). Further AFM analysis suggested that contribution of actomyosin as revealed with apical surface softening by blebbistatin and stiffness of dissociated NPCs were both comparable between pallium and GE, not accounting for the differential apical surface stiffness. We then found that the density of apices of NPCs was greater, with denser F-actin meshwork, in the apically stiffer pallium than in GE. A similar correlation was found between the decreasing density between E12 and E14 of NPC apices and the declining apical surface stiffness in the same period in both the pallium and the GE. Thus, one plausible explanation for the observed difference (pallium > GE) in apical surface stiffness may be differential densification of NPC apices. In laser ablation onto the apical surface, the convex-shaped GE apical surface showed quicker recoils of edges than the pallial apical surface did, with a milder inhibition of recoiling by blebbistatin than in pallium. This greater pre-stress in GE may provide an indication of how the initially apically concave wall then becomes an apically convex “eminence. ”
The Maximum Duo-Preservation String Mapping Problem with Bounded Alphabet

Given two strings A and B such that B is a permutation of A , the max duo-preservation string mapping (MPSM) problem asks to find a mapping π between them so as to preserve a maximum number of duos. A duo is any pair of consecutive characters in a string and it is preserved by π if its two consecutive characters in A are mapped to same two consecutive characters in B . This problem has received a growing attention in recent years, partly as an alternative way to produce approximation algorithms for its minimization counterpart, min common string partition , a widely studied problem due its applications in comparative genomics. Considering this favored field of application with short alphabet, it is surprising that MPSM ℓ , the variant of MPSM with bounded alphabet, has received so little attention, with a single yet impressive work that provides a 2 . 67-approximation achieved in O ( n ) [5], where n = | A | = | B | . Our work focuses on MPSM ℓ , and our main contribution is the demonstration that this problem admits a Polynomial Time Approximation Scheme (PTAS) when ℓ = O (1). We also provide an alternate, somewhat simpler, proof of NP-hardness for this problem compared with the NP-hardness proof presented in [16].
Modeling Whale Deaths From Vessel Strikes to Reduce the Risk of Fatality to Endangered Whales

Vessel strikes have been documented around the world and frequently figure as a top human cause of large whale mortality. The shipping lanes in the Santa Barbara Channel, California and nearby waters have some of the highest predicted whale mortality from vessel strikes in the United States waters of the eastern Pacific. Beginning in 2007, National Oceanographic and Atmospheric Administration requested voluntary vessel speed reductions (VSRs) for vessels greater than 300 GT traveling in the Santa Barbara Channel shipping routes to decrease whale mortality from ship strikes. We employed a ship strike model using whale density data and automatic identification system (AIS) vessel data to estimate mortality under several management scenarios. To assess the effect of the VSR on strike mortality, we bootstrapped speeds from vessels greater than 19 m long that transited when no VSR was in place. Finally, we calculated the predicted mortality for hypothetical cooperation scenarios by artificially adding speed caps post-hoc to real vessel transits. For 2012–2018, we estimated that in our study area on average during summer/fall (June–November) 8.9 blue, 4.6 humpback, and 9.7 fin whales were killed from ship strikes each year (13–26% greater than previously estimated). We evaluated winter/spring (January–April) humpback mortality for the first time, resulting in an estimate of 5.7 deaths on average per year. Poor cooperation with the VSR led to low (5% maximum) to no reductions in the estimated number of strike mortalities. Evaluating potential scenarios showed that if 95% cooperation occurred in the lanes, whale deaths there would decrease by 22–26%. Adding VSRs with similar cooperation levels at the northern end of the Santa Barbara Channel and south of Channel Islands National Marine Sanctuary could decrease estimated strike mortalities in those areas by 30%. If VSRs were added and cooperation reached 95% there and in the lanes, we estimate a 21–29% decrease in vessel strike mortalities could be attained relative to estimated mortality in the entire study area. To decrease the vessel strike related whale mortalities in this region, we recommend expanding the VSR areas and increasing cooperation voluntarily, or considering mandatory speed limits if desired cooperation levels cannot otherwise be met.