Miracle-dz/UniFAR-FTUs huggingface dataset:
Designing Novel Subunit Vaccines against Herpes Simplex Virus-1 using Reverse Vaccinology Approach

Herpes Simplex Virus (HSV) is an infectious virus that is responsible for various types of orofacial and genital infections. Two types of HSV viruses, HSV-1 and HSV-2, are the most dangerous HSV viruses. Every year, millions of people get infected with this menacing virus, however, no satisfactory treatment or vaccine has not yet been discovered to fight against HSV. Although there are some anti-viral therapies, however, studies have showed that such anti-viral therapies may also fail to provide good impact. In this study, a possible subunit vaccine against HSV-1, strain-17, was designed using the tools and reverse vaccinology and bioinformatics. Three potential antigenic envelope glycoproteins were selected from nine envelope glycoproteins, for possible vaccine construction. Potential epitopes capable of inducing high immunogenic response and at the same time have non-allergenicity and conservancy across other strains and species, were selected by some robust analysis, for vaccine construction. Finally, three possible vaccines were designed. Each of the vaccine construct differ from each other only in their adjuvant sequences and based on molecular docking analysis, one best vaccine construct was selected for molecular dynamics simulation study and in silico codon adaptation. The experiment showed that the selected best vaccine should be good candidate against HPV-1, strain-17. However, wet lab study should be conducted on the suggested vaccine(s) for confirming their potentiality, safety and efficacy.
Aac Strategies For Individuals With Moderate To Severe Disabilities

Thank you very much for reading aac strategies for individuals with moderate to severe disabilities. As you may know, people have search numerous times for their favorite novels like this aac strategies for individuals with moderate to severe disabilities, but end up in infectious downloads. Rather than reading a good book with a cup of coffee in the afternoon, instead they cope with some infectious bugs inside their laptop.
Smart therapeutic-polymer-loaded silica nanomaterials. Nanomedicine

ABSTRACT Need for materials with high biocompatible properties have led to the development of porous silica nanoparticles. The structure of present nanostructures consists of a core and a silica shell or the silica core is coated with organic shell. The composite nanoparticles coated by organic shells with functional groups were considered to govern the covalent immobilization of biomolecules. The nanoparticles with unique chemical and physical properties may be useful as biosensors in living whole cells. The enhanced cellular drug delivery to cancer cell lines via nanoconjugates revealed that smart nanoparticles are an effective tool for transporting and delivering drugs. Graphical abstract Model for synthesis of silica nanoparticles by a reverse phase microemulsion.
Clinical and molecular epidemiology of invasive Staphylococcus aureus infection in Utah children; continued dominance of MSSA over MRSA

Background Invasive Staphylococcus aureus infections are a common cause of morbidity and mortality in children. In the early 2000’s the proportion of infections due the methicillin-resistant S. aureus (MRSA) increased rapidly. We described the clinical and molecular epidemiology of invasive S. aureus disease in a pediatric population. Methods We prospectively identified children in Utah with invasive S. aureus infections. Medical records were reviewed to determine diagnosis and clinical characteristics. Isolates were genotyped using multi-locus sequence typing. The presence of genes encoding the Panton-Valentine leukocidin (PVL) was determined using polymerase chain reaction. Results Over a 4-year period between January 2009 and December 2012, we identified 357 children, hospitalized at Primary Children’s Hospital, with invasive S. aureus infections and isolates available for the study. Methicillin-susceptible S. aureus (MSSA) caused 79% of disease, while MRSA caused only 21% of disease. Mortality associated with invasive S. aureus infection was 3.6%. The most common diagnoses were osteoarticular infections (38%) followed by central line associated blood stream infections (19%) and pneumonia (12%). We identified 41 multi-locus sequence types. The majority of isolates belonged to 6 predominant clonal complexes (CC5, CC8, CC15, CC30, CC45, CC59). PVL was present in a minority (16%) of isolates, of which most were ST8 MRSA. Conclusions MSSA was the primary cause of invasive S. aureus infections at our institution throughout the study period. A limited number of predominant strains accounted for the majority of invasive disease. The classic virulence factor PVL was uncommon in MSSA isolates. Further study is needed to improve our understanding of S. aureus virulence and disease pathogenesis.
Natural Variability in a Changing Ocean: Emergence and Impacts

Anthropogenically-forced changes in the ocean are underway and critical for the ocean’s role as a carbon sink and marine habitat. Detecting such changes will require quantification of not only the magnitude of the change (anthropogenic signal) but also the natural variability inherent to the climate system (noise). This work uses Earth System Models (ESMs) to (1) evaluate timescales over which anthropogenic signals in the contemporary ocean emerge from natural climate variability and (2) interpret observed variability in the Pacific Basin. We apply time-of-emergence (ToE) diagnostics to a Large Ensemble experiment of an ESM, providing both a conceptual framework for interpreting the detectability of anthropogenic impacts on the ocean carbon cycle and observational sampling strategies required to achieve detection. We find ToEs for di↵erent components of the ocean carbon cycle range from under a decade to over a century, a consequence of the time-lag between chemical and radiative impacts of rising atmospheric CO2 on the ocean. Processes sensitive to chemical changes emerge rapidly, such as impacts of acidification on the calcium-carbonate pump (10-20 years), and the invasion of anthropogenic CO2 into the ocean (20-30 years). Processes sensitive to the ocean’s physical state, such as the soft-tissue pump, which depends on nutrients supplied through circulation, emerge decades later (20-80+ years). Next, we evaluate the modeland scenario-sensitivity of ToEs through comparing Large Ensembles from four ESMs. We find ToEs are robust across models for variables that are tied directly with the rise in atmospheric CO2 namely rising sea surface temperature and the invasion of anthropogenic CO2 into the ocean (ToE 2030 years). For the soft-tissue pump, ocean color, and sea surface salinity, ToEs are longer (50+ years), less robust across the ESMs, and more sensitive to the forcing scenario considered.
PLAGIARISM IN ACADEMIC WRITING AMONG STUDENTS OF HIGHER LEARNING INSTITUTIONS IN MALAYSIA: AN ISLAMIC PERSPECTIVE

With the emergence of cyber technology used as a learning tool, be it in the form of e-learning or as a means for finding and sharing information, many academic institutions are faced with unethical behaviors and misuse of technology, which has been an area of concern for a considerable number of years. Plagiarism and academic dishonesty have been discussed through various perspectives among educators, researchers, educational policy-makers, as well as media reporters. Yet, the problem remains unsolved indicating that either the issue has not been fully understood to the extent that can lead to an appropriate approach, or that the strategies developed have not been appropriately and sufficiently implemented. This paper engaged a mixed method of quantitative and qualitative enquiries to triangulate the findings in the attempt to investigate the factors that influence plagiarism and develop strategies to minimize this behavior, u sing Ajzen’s Theory of Planned Behavior (TPB ), this time viewed from an Islamic perspective. The questionnaire survey was administered in four selected universities in Malaysia and involved a sample of 400 students, while the qualitative procedures included semi-structured interviews with five post graduate students and an open-ended survey. The analysis revealed that besides the original elements of the TPB there were other newly emerging elements, among which the element of Religiosity was visualized as an umbrella and had its influence on every other aspect of the issue. The revised model is provided with the hope that it offers strong implications for educational policy makers, academics as well as students in their quest to minimize plagiarism and academic dishonesty, and to also accomplish the aspiration for quality writing in higher learning institutions .
All-digital half-rate referenceless CDR with single direction frequency sweep scheme using asymmetric binary phase detector

This paper presents an all-digital half-rate referenceless CDR with a single direction frequency acquisition achieved. Thanks to asymmetric binary phase detector (ABPD), compared with existed inverse alexander phase detector (IAPD), we changed the input-output characteristic to enable accumulated phase error point in the same direction when frequency error happens while the output of IAPD has no directivity. Once the frequency of the digital controlled oscillator (DCO) enters the pull-in range of the CDR loop, the phase is automatically locked. To improve jitter tolerance (JTOL) performance further, IAPD logic is enabled, and ABPD is disabled after locking. The prototype was implemented in a 28nm low power CMOS process with a data rate tracking range of 9 – 11 Gb / s. The measured JTOL is 0.35 UI at high frequency with PRBS31 input data pattern. Keywords: clock and data recovery (CDR), frequency acquisition, refer-enceless, jitter tolerance (JTOL), referenceless CDR, digitally controlled oscillator (DCO)
Social Capital along Wine Trails: Spilling the Wine to Residents?

Social capital is a vital element of tourism development and sustainability, and has thus drawn significant attention during the past decade. Yet, this topic is still under-researched in the context of niche tourism, especially along linear tourism settings such as wine trails. As a major component of wine tourism, wine trails have been growing rapidly worldwide to further regional tourism development. To examine the level of social capital related with wine tourism, communities surrounding two wine trails in North Carolina (U.S.) were surveyed regarding the Trust, Information Sharing, Collective Action, Bonding and Bridging dimensions of social capital. Results indicated the Piedmont region has not yet fully developed the social capital associated with local wine trails, although residents perceived somewhat strong Collective Action derived from this growing tourism niche. Visitation frequency to wine trails was found to be significantly associated with all dimensions of wine tourism social capital. This study advances the wine tourism scholarship concerning social capital along wine trails (e.g., integrating main dimensions into one scale). It also sheds light on wine trail development and management, suggesting local wineries and wine trails invest greater effort in forging community bonds, especially among older residents, and bridging with local businesses.
Characteristics Of "mobile" And "stable" Occupational Educators By Specialty And By Type Of School

AUTHOR Wiens, A. Emerson TITLE The Characteristics of "Mobile" and "Stable" Occupational Educators by Specialty and by Type of School. INSTITUTION Illinois Univ., Urbana. Bureau of Educational Research. -SPONS AGENCY Illinois State Board of Vocational Education and Rehabilitation, Springfield. Div. of Vocational and Technical Education. ; Office of Education (DHEW), Washington, D.C. PUB DATE Nov 73 CONTRACT OEC-PDT-A3-063 NOTE 368p.
Identifikasi Etnomatematika Pada Masjid Mataram Kotagede Yogyakarta

Abstract Indonesia is a nation that is rich in cultural diversity, but the development of globalization has become a major challenge in maintaining its sustainability. Culture-based learning is one solution to this problem. One of the studies of learning mathematics based on culture is ethnomatematics. The purpose of this study is to identify ethnomatematics of historical sites or cultural heritage buildings in the Yogyakarta Kotagede Mataram Mosque which can be used as a medium of mathematics learning, especially in learning Geometry. This research is a descriptive study with ethnographic approach. Based on observations obtained several mathematical concepts contained in the parts of the Mataram Kotagede Yogyakarta mosque which are displayed in the form of a matrix. The results showed that the parts of the Yogyakarta Kotagede Mataram Mosque related to mathematical concepts include carvings (ornaments) on walls, pavilion buildings, roofs of mosques, drum which closely relates to geometrical concepts including flat building and building. Keywords : ethnomatematics, culture, mathematics Abstrak Indonesia merupakan bangsa yang kaya akan keanekaragaman budaya, namun perkembangan arus globalisasi menjadi tantangan utama dalam menjaga kelestariannya. Pembelajaran berbasis budaya menjadi salah satu solusi permasalahan ini. Salah satu kajian pembelajaran matematika berbasis budaya adalah etnomatematika. Tujuan dari penelitian ini untuk mengidentifikasi etnomatematika situs-situs sejarah atau bangunan cagar budaya yang terdapat di Masjid Mataram Kotagede Yogyakarta yang dapat dijadikan sebagai media pembelajaran matematika khususnya dalam pembelajaran Geometri. Penelitian ini merupakan penelitian deskriptif dengan pendekatan etnografi. Berdasarkan hasil observasi di peroleh beberapa konsep matematika yang terdapat pada bagian-bagian bangunan masjid Mataram Kotagede Yogyakarta yang ditampilkan dalam bentuk matriks. Hasil Penelitian menunjukkan bahwa bagian-bagian pada Masjid Masjid Mataram Kotagede Yogyakarta yang berkaitan konsep matematika antara lain ukiran-ukiran (ornamen) pada dinding, bangunan pendopo, atap masjid, beduk yang erat kaitanya dengan konsep geometri diantaranya bagun datar dan bangun ruang. Kata Kunci : etnomatematika, budaya, matematika
A pragmatic randomized controlled trial to compare a novel group physiotherapy programme with a standard group exercise programme for managing chronic low back pain in primary care

Objectives: To compare a novel group physiotherapy exercise programme with a standard programme for managing chronic low back pain (CLBP) in primary care. Introduction: Group exercise programmes used in physiotherapy practice are a cost effective treatment for managing CLBP but currently lack a combination of individualized specific exercises, one-to-one education and manual therapy. A novel group exercise programme was designed to address these limitations. Methods: The novel group exercise programme (Group A) was compared to a standard group exercise programme used in clinical practice (Group B) in a pragmatic randomized controlled trial with focus groups. Participants with CLBP were allocated to the two programme groups by block randomization. Participants in both groups attended six one-hour programme sessions over a 3-month period. Outcomes measuring function (Functional Rating Index (FRI)), pain (Numerical Pain Rating Scale (NPRS)), quality of life (EQ-5D) and satisfaction with treatment were used to evaluate the effectiveness of the programmes pre- and post-programme attendance and at 6 months. Focus groups were used to explore patients’ experiences regarding their treatment in the two group programmes. Results: Eighty-one participants were randomized to the two groups (41 in Group A; 40 in Group B). Both groups showed improvements to function, quality of life, and pain post treatment. There were no significant between-group differences in any outcomes post treatment and small effect sizes were found. Quality of life deteriorated in Group B at 6 months but continued to improve in the novel group. The within group analysis revealed significantly lower FRI and NPRS scores post-programme compared to pre-programme in both groups but these improvements were greater in the novel programme. EQ-5D scores were significantly higher in the novel programme post treatment but not in Group B. The focus group interviews showed that these group programmes provide social support but participants would like further guidance post treatment. Patients prefer individualized exercises as well as one-to-one education which are components of the novel programme. Conclusion: This novel programme may provide a suitable addition to existing programmes available for managing CLBP. It is recommended that this programme be further evaluated in clinical practice.
Hierarchical assembly of the MLL1 core complex within a biomolecular condensate regulates H3K4 methylation

The enzymes that regulate histone H3 lysine 4 (H3K4) methylation are required for cellular differentiation and development and are often mutated in human disease. Mixed Lineage Leukemia protein-1 (MLL1) is a member of the SET1 family of histone H3 lysine 4 methyltransferases, which require interaction with a conserved sub-complex consisting of WDR5, RbBP5, Ash2L and DPY30 (WRAD2) for maximal activity. It is currently unclear how assembly of SET1 family complexes is involved in the spatiotemporal control of H3K4 methylation in eukaryotic genomes. In this investigation, we systematically characterized the hydrodynamic and kinetic properties of a reconstituted human MLL1 core complex and found that its assembly is highly concentration and temperature dependent. Consistent with a hierarchical assembly pathway, we found that the holo-complex assembles through interactions between the MW and RAD2 sub-complexes, which is correlated with enzymatic activity. Surprisingly, we found that the disassembled state is favored at physiological temperatures, and that this thermodynamic barrier can be overcome under conditions that induce high-local concentrations of subunits in phase separated compartments. Combining this data with the observation that MLL1 primary sequence contains large regions of intrinsic disorder, we propose a “swinging-domain” model in which the interaction between a tethered MW subcomplex and multiple nucleosome-RAD2 complexes is regulated by the rapid formation or dissolution of biomolecular condensates, such as occurs in transcription factories. This model provides an elegant “switch-like” mechanism for spatiotemporal control of H3K4 methylation within eukaryotic genomes.
Polarization of Tastes: Stated Preference Stability in Sequential Discrete Choices

Purpose: The aim of this study is to assess stated preference stability in long-format discrete choice experiments. As the number of choice situations increases, data reveal more precise information regarding preferences. However, there are many doubts concerning the incentives compatibility of long designs. Psychological effects such as respondents’ learning, fatigue and decreasing concentration in successive choice situations can result in biased estimators of parameters of utility functions. It is, not clear which group of successive choices reveal the most trustworthy information: the initial choices (undistorted but potentially not robust) or a later set (consciously formed preferences but potentially under conditions of fatigue). Design/Methodology/Approach: With the long-format (144 choice tasks) data concerning employment options, we estimated utility function parameters were estimated using MNL and MMNL models.To conduct inter- intra- respondent analysis we used imputed individual-level parameters of utility function. Findings: We show that preferences are formulated at the intra-respondent level according to a specific pattern and, at the same time, the preferences of single respondents show lower variance across choice tasks than across populations. An increase in the standard deviation of parameters across the sample does not necessarily mean an inconsistency of preferences in this type of study. This can result from polarization of preferences in the population with simultaneous intra-respondent preference consistency. Practical Implications: Long-format DCEs can reveal some of the behavioural mechanisms behind the decision-making process. We show that, using this kind of study, it is possible to observe preference formulation. In some specific cases obtaining accurate information, or even teaching respondents their preferences, can be of a substantial significance. Originality/Value: An increase in the standard deviation of parameters across the population does not necessarily mean inconsistency resulting from the 'negative' consequences of ordering effects, contrary to the findings of Swait and Adamowicz (2001). Instead, this can result from the polarization of preferences in the population alongside the intra-respondent consistency.
Tracing Evolutionary Ages of Cancer-Driving Sites by Cancer Somatic Mutations

Evolutionary understanding of cancer genes may provide insights on the nature and evolution of complex life and the origin of multicellularity. In this study, we focus on the evolutionary ages of cancer-driving sites, and try to explore to what extent the amino acids of cancer-driving sites can be traced back to the most recent common ancestor (MRCA) of the gene. According to gene phylostraigraphy analysis, we use the definition of gene age (tg) by the most ancient phylogenetic position that can be traced back, in most cases based on the large-scale homology search of protein sequences. Our results are shown that the site-age profile of cancer-driving sites of TP53 is correlated with the number of cancer types the somatic mutations may affect. In general, those amino acid sites mutated in most cancer types are much ancient. These sites frequently mutated in cancerous cells are possibly responsible for carcinogenesis; some may be very important for basic growth of single-cell organisms, and others may contribute to complex cell regulation of multicellular organisms. The further cancer genomics analysis also indicates that ages of cancer-driving sites are ancient but may have a broad range in early stages of metazoans.