Miracle-dz/UniFAR-FTUs huggingface dataset:
Multiwavelength Variability Power Spectrum Analysis of the Blazars 3C 279 and PKS 1510–089 on Multiple Timescales

We present the results of variability power spectral density (PSD) analysis using multiwavelength radio to GeV γ-ray light curves covering timescales of decades/years to days/minutes for the blazars 3C 279 and PKS 1510−089. The PSDs are modeled as single power laws, and the best-fit spectral shape is derived using the “power spectral response” method. With more than 10 yr of data obtained with weekly/daily sampling intervals, most of the PSDs cover ∼2–4 decades in temporal frequency; moreover, in the optical band, the PSDs cover ∼6 decades for 3C 279 due to the availability of intranight light curves. Our main results are the following: (1) on timescales ranging from decades to days, the synchrotron and the inverse-Compton spectral components, in general, exhibit red-noise (slope ∼2) and flicker-noise (slope ∼1) type variability, respectively; (2) the slopes of γ-ray variability PSDs obtained using a 3 hr integration bin and 3 weeks total duration exhibit a range between ∼1.4 and ∼2.0 (mean slope = 1.60 ± 0.70), consistent within errors with the slope on longer timescales; (3) comparisons of fractional variability indicate more power on timescales ≤100 days at γ-ray frequencies compared to longer wavelengths, in general (except between the γ-ray and optical wavelengths for PKS 1510−089); (4) the normalization of intranight optical PSDs for 3C 279 appears to be a simple extrapolation from longer timescales, indicating a continuous (single) process driving the variability at optical wavelengths; and (5) the emission at optical/infrared wavelengths may involve a combination of disk and jet processes for PKS 1510−089.
A geometric perspective on the scaling limits of critical Ising and $\varphi^4_d$ models

The lecture delivered at the \emph{Current Developments in Mathematics} conference (Harvard-MIT, 2021) focused on the recent proof of the Gaussian structure of the scaling limits of the critical Ising and $\varphi^4$ fields in the marginal case of four dimensions(joint work with Hugo Duminil-Copin). These notes expand on the background of the question addressed by this result, approaching it from two partly overlapping perspectives: one concerning critical phenomena in statistical mechanics and the other functional integrals over Euclidean spaces which could serve as a springboard to quantum field theory. We start by recalling some basic results concerning the models' critical behavior in different dimensions. The analysis is framed in the models' stochastic geometric random current representation. It yields intuitive explanations as well as tools for proving a range of dimension dependent results, including: the emergence in $2D$ of Fermionic degrees of freedom, the non-gaussianity of the scaling limits in two dimensions, and conversely the emergence of Gaussian behavior in four and higher dimensions. To cover the marginal case of $4D$ the tree diagram bound which has sufficed for higher dimensions needed to be supplemented by a singular correction. Its presence was established through multi-scale analysis in a recent joint work with HDC.
Common Trajectories of Highly Effective CD19-Specific CAR T Cells Identified by Endogenous T-cell Receptor Lineages

Current chimeric antigen receptor-modified (CAR) T cell therapy products are evaluated in bulk, without assessment of the possible heterogeneity in effector potential between cells. Conceivably, only a subset of the pre-infusion product differentiates into optimal effectors. We generated a comprehensive single-cell gene expression and T cell receptor (TCR) sequencing dataset using both pre- and post-infusion CD19-CAR T cells from peripheral blood and bone marrow of pediatric patients with B cell acute lymphoblastic leukemia (B-ALL). We identified potent effector post-infusion cells with identical TCRs to a subset of pre-infusion CAR T cells. Effector precursor CAR T cells exhibited a unique transcriptional profile compared to other pre-infusion cells, and the number of effector precursor cells infused correlated with peak CAR T cell expansion. Additionally, we identified an unexpected cell surface phenotype (TIGIT+, CD62Llo, CD27-), conventionally associated with inhibiting effective T cell responses, that we used to successfully enrich for subsequent effector potential. Collectively, these results demonstrate that highly diverse effector potentials are present among cells in pre-infusion cell products, which can be exploited for diagnostic and therapeutic applications. Furthermore, we provide an integrative experimental and analytical framework for elucidating the biological mechanisms underlying effector development in other CAR T cell therapy products.
A high-throughput amplicon screen for somatic UBA1 variants in Cytopenic and Giant Cell Arteritis cohorts

Somatic mutations in the gene encoding the major E1 ubiquitin ligase, UBA1, were recently identified as a cause of VEXAS, a late-onset acquired auto-inflammatory syndrome. Differential diagnoses for patients subsequently found to have VEXAS include relapsing polychondritis, Sweets syndrome, myelodysplastic syndrome (MDS), giant cell arteritis (GCA) and undifferentiated systemic autoinflammatory disease (uSAID). We therefore sought to screen DNA from individuals with a non-diagnostic cytopenia or GCA, for known VEXAS-associated mutations. To this end, we developed a multiplexed UBA1 amplicon sequencing assay, allowing quick screening of large cohorts while also providing sufficient sequencing depth to identify somatic mutations to an allele frequency <1%. Using this assay, we screened genomic DNA from 612 males diagnosed with GCA, and bone marrow DNA from 1,055 cases with an undiagnosed cytopenia. No GCA cases were found to have UBA1 mutations, however 4 different mutations in the cytopenic cohort were identified in 7 individuals. Furthermore, we describe a female case identified in the screen with a UBA1 mutation and all VEXAS-associated phenotypes, but without Monosomy X. Our study suggests that, despite the overlap in clinical features, VEXAS is rarely misdiagnosed as GCA, but identified in 1.0% of males with an undiagnosed cytopenia. The identification of a UBA1 variant in a female case adds further evidence that VEXAS should not be ruled out as a differential diagnosis in females with VEXAS-like symptoms.
Macro-Econometric System Modelling @ 75

We summarize the history of macroeconometric system modelling as having produced four generations of models. Over time the principles underlying the model designs have been extended to incorporate eight major features. Because models often evolve in response to external events we are led to ask what has happened to models used in the policy process since the financial crisis on 2008/9. We find that models have become smaller but that there is still no standard way of capturing the effects of such a crisis.
Development of Smart Missile Fins with Active Spoiler

For conventional missiles, electric or hydraulic actuators are mounted inside the missile fuselage to activate the aerodynamic control surfaces. These internally mounted actuators occupy considerable volume which otherwise can be used for payload or additional fuel. Reducing the size of the internal actuators and hence lowering the total actuator weight may improve the overall performance of missile significantly. The goal of this research is to develop a light-weight, low cost smart missile fin capable of surviving the supersonic operating environment while providing necessary performance comparable to existing missile fins. In this study, in an alternative to facilitate realistic design concepts and to generate enough actuation forces required for missile controls, smart fins with trailing-edge-mounted retractable wedge are considered. The retractable wedge stretches in or out appropriately to decrease the applied pitching moment of the missile fin. For the theoretical calculation, a commercial CFD software package is used to obtain the forces and the moments generated by the fin with retractable wedge. The piezoelectric actuation mechanism that is applicable to the wedge type actuator is also investigated.
Humoral and cellular responses to SARS-CoV-2 vaccination in patients with lymphoid malignancies

Protection against COVID-19 is dependent on vaccination. Antibodies and antigen-specific T-cell responses against the spike domain can be used to measure vaccine immune response. Individuals with lymphoma have defects in humoral and cellular immunity that may compromise vaccine response. In this prospective observational study of 457 participants with lymphoma, 52% of participants vaccinated on treatment had undetectable anti-spike IgG antibodies compared to 9% who were not on treatment. Marked impairment was observed in those receiving anti-CD20 antibody within 12 months where 60% had undetectable antibodies compared to 11% on chemotherapy, which persisted despite three vaccine doses. Overall, 63% had positive T-cell responses irrespective of treatment. Individuals with indolent B-cell lymphoma have impaired antibody and cellular responses that were independent of treatment. Altogether, the significant reduction and heterogeneity in immune responses in these patients emphasise the urgent need for antibody monitoring and alternative prophylactic strategies to protect against COVID-19.
Accelerating Dynamical System Simulations with Contracting and Physics-Projected Neural-Newton Solvers

Recent advances in deep learning have allowed neural networks (NNs) to successfully replace traditional numerical solvers in many applications, thus enabling impressive computing gains. One such application is time domain simulation, which is indispensable for the design, analysis and operation of many engineering systems. Simulating dynamical systems with implicit Newton-based solvers is a computationally heavy task, as it requires the solution of a parameterized system of differential and algebraic equations at each time step. A variety of NN-based methodologies have been shown to successfully approximate the trajectories computed by numerical solvers at a fraction of the time. However, few previous works have used NNs to model the numerical solver itself. For the express purpose of accelerating time domain simulation speeds, this paper proposes and explores two complementary alternatives for modeling numerical solvers. First, we use a NN to mimic the linear transformation provided by the inverse Jacobian in a single Newton step. Using this procedure, we evaluate and project the exact, physics-based residual error onto the NN mapping, thus leaving physics ``in the loop''. The resulting tool, termed the Physics-pRojected Neural-Newton Solver (PRoNNS), is able to achieve an extremely high degree of numerical accuracy at speeds which were observed to be up to 31% faster than a Newton-based solver. In the second approach, we model the Newton solver at the heart of an implicit Runge-Kutta integrator as a contracting map iteratively seeking a fixed point on a time domain trajectory. The associated recurrent NN simulation tool, termed the Contracting Neural-Newton Solver (CoNNS), is embedded with training constraints (via CVXPY Layers) which guarantee the mapping provided by the NN satisfies the Banach fixed-point theorem.
Implementing New Generation Protective Relay Schemes based on IEC61850 Standard for Substation Communication in the Eskom 765kV Transmission Network

Eskom is in the process of strengthening the transmission corridor between the North and South of South Africa by constructing a new 765 kV backbone to meet the challenge of increased electricity demand. Eskom's existing substation layouts are based on double busbar arrangements. The approved new extra high voltage (EHV) substations (765 kV and 400 kV) will be based on a breaker-and-a-half substation design. Associated with this upgrade, new generation protective relay schemes for transmission lines (both line impedance and line differential), transformers, reactors and busbars will be implemented. The paper gives an overview of the new substation layout as well as the protective relay scheme architecture. The advantages of using the IEC61850 based protocol between the bay and substation control as well as GOOSE messaging for control of the breaker-and-a-half schemes will be discussed. The paper also describes the test philosophy and how GOOSE messages have been configured to improve the speed and efficiency of commissioning and testing the various schemes. The test procedures as well as test system to achieve a fully automatic test of the complete scheme will be presented.
Don’t Tate Us! The impediments and drivers of branding museums

This study examines the applicability of brand orientation, and its drivers and impediments in the museum context. A multiple case study approach, with 12 institutions across two countries (United Kingdom and Australia) was carried out. Results suggest that brand orientation provides the cultural platform to retain the distinctiveness of the institution and build the symbolic representation required to remain competitive in the future.
Analysis of drivers' preparatory behaviour before turning at intersections

This study analyses naturalistic driving behaviour before making turns at intersections. Instrumented vehicles recorded drivers' preparatory behaviour, including moving the right foot to cover the brake pedal and activating the turn-signal, while approaching the turning point on public roads. Data were analysed for relations between driver preparations, different traffic conditions (with or without forward and/or following vehicles) and the intersection type and turning direction. The results suggest that braking began closer to the target intersection when driving with a vehicle in front, independent of the number of traffic lanes or the turning direction. In contrast, the onset of braking occurred further before the intersection when drivers had a forward vehicle while approaching intersections after curves, where drivers cannot readily determine the distance between their vehicle and the turning point until just before the intersection. The presentation timing of vehicle navigation systems for each traffic condition is discussed based on the road structures of turning points.
Azotobacter vinelandii scaffold protein NifU transfers iron to NifQ as part of the iron-molybdenum cofactor biosynthesis pathway for nitrogenase

Azotobacter vinelandii molybdenum-dependent nitrogenase obtains molybdenum from NifQ, a monomeric iron-sulfur molybdoprotein. This protein requires of a preexisting [Fe-S] cluster to form a [MoFe3S4] group to serve as specific donor during nitrogenase cofactor biosynthesis. Here, we show biochemical evidence for NifU being the donor of the [Fe-S] cluster. Protein-protein interaction studies using apo-NifQ and as-isolated NifU demonstrated the interaction between both proteins which is only effective when NifQ is unoccupied by its [Fe-S] cluster. The apo-NifQ iron content increased after the incubation with as-isolated NifU, reaching similar levels to holo-NifQ after the interaction between apo-NifQ and NifU with reconstituted transient [Fe4-S4] groups. These results also indicate the necessity of co-expressing NifU together with NifQ in the pathway to provide molybdenum for the biosynthesis of nitrogenase in engineered nitrogen-fixing plants.
Ca2+ Mediates HIF-dependent Upregulation of Aquaporin 1 in Pulmonary Arterial Smooth Muscle Cells

Prolonged exposure to hypoxia causes structural remodeling and sustained contraction of the pulmonary vasculature, resulting in the development of pulmonary hypertension. Both pulmonary arterial smooth muscle cell (PASMC) proliferation and migration contribute to the vascular remodeling. We previously showed that the protein expression of aquaporin 1 (AQP1), a membrane water channel protein, is elevated in PASMCs during following in vivo or in vitro exposure to hypoxia. Studies in other cell types suggest that AQP1 is a direct transcriptional target of hypoxia inducible factor (HIF)-1. Moreover, we and others have shown that an increase in intracellular calcium concentration ([Ca2+]i) is a hallmark of hypoxic exposure in PASMCs. Thus, we wanted to determine whether HIF regulates AQP1 in PASMCs and, if so, whether the process occurred via transcriptional regulation or was Ca2+-dependent. PASMCs were exposed to hypoxia, incubated with DMOG, which inhibits HIFα protein degradation or infected with constitutively active forms of HIF-1α or HIF-2α. Hypoxia, DMOG and HIF1/2α produced a time-dependent increase in AQP1 protein, but not mRNA. Interestingly, incubation with increasing HIF1/2a levels and DMOG increased [Ca2+]i in PASMCs, and this elevation was prevented by the voltage-gated Ca2+ channel inhibitor, verapamil (VER) and nonselective cation channel inhibitor SKF96365 (SKF). VER and SKF also blocked upregulation of AQP1 protein by DMOG or HIF1/2α, but had no effect on expression of GLUT1, a canonical HIF transcriptional target. Silencing of AQP1 abrogated increases in PASMC migration and proliferation induced by HIF1/2α, suggesting induction of AQP1 protein by HIF1/2α has a functional outcome in these cells. Thus, our results show that contrary to reports in other cell types, in PASMCs, AQP1 does not appear to be a direct target for HIF transcriptional regulation. Instead, AQP1 protein may be upregulated by a mechanism involving HIF-dependent increases in [Ca2+]i.
Sexual and developmental variations of ecto-parasitism in damselflies

The prevalence and intensity of parasitism can have different fitness costs between sexes, and across species and developmental stages. This variation could arise because of species specific sexual and developmental differences in body condition, immunity, and resistance. Theory predicts that the prevalence of parasitism will be greater in individuals with poor body condition and the intensity of parasitism will be greater in individuals with larger body size. These predictions have been tested and verified in vertebrates. In insects, however, contradictory evidence has been found in different taxa. Here, we tested these predictions on two species of Agriocnemis (Agriocnemis femina and Agriocnemis pygmaea) damselflies, which are parasitized by Arrenurus water mite ectoparasites. We measured body weight, total body length, abdomen area and thorax area of non-parasitized damselflies and found body condition varied between males and females, between immature females and mature females and between A. femina and A. pygmaea. Then, we calculated the parasite prevalence, i.e., the frequency of parasitism and intensity, i.e., the number of parasites per infected damselfly in eleven natural populations of both species. In line to our predictions, we observed greater prevalence in immature females than mature females but found no difference in parasite prevalence between males and females. Furthermore, we found that parasite load was higher in females than males and in immature females than mature females. Our result also showed that the frequency and intensity of parasitism varied between the two studied species, being higher in A. pygmaea than A. femina. Our study provides evidence that parasitism impacts sexes, developmental stages and species differentially and suggests that variation may occur due to sex, developmental stage, and species-specific resistance and tolerance mechanism.
Microstructure refinement mechanism of undercooled Cu55Ni45 alloys

Abstract Different undercooling degrees of Cu55Ni45 alloy were obtained by the combination of molten glass purification and cyclic superheating, and the maximum undercooling degree reached 284 K. The microstructure of the alloy was observed by metallographic microscope, and the evolution of microstructure was studied systematically. There are two occasions of grain refinement in the solidification structure of the alloy: one occurs in the case of low undercooling, and the other occurs in the case of high undercooling. Electron backscatter diffraction (EBSD) technology was used to analyze the rapid solidification structure under high undercooling. The features of flat polygonal grain boundary, high proportion of twin boundary, and large proportion of large angle grain boundary indicate recrystallization. The change in microhardness of the alloy under different undercooling degrees was studied by microhardness tester. It was found that the average microhardness decreased sharply at high undercooling degrees, which further confirmed the recrystallization of the solidified structure at high undercooling degrees.
Increasing the Efficiency of a Robotic Cell Using Simulation

Abstract The paper deals with the possibilities of increasing the efficiency of a robotic cell. The robot assembly station is a part of a multi-robotic workplace capable of performing solitary operations. The complete production cycle of the cell is subjected to a thorough analysis. Deficiencies, having a direct impact on cell efficiency, are identified. These shortcomings include redundant movements of the robotic arm, the absence of an inspection mechanism for the presence of assembly parts, and inefficient instructions of the production cycle algorithm. The identified deficiencies are eliminated using the CIROS simulation tool. The result of the adjustments is a global 11.4% increase in the efficiency of the robotic cell in terms of time performance.
Assessing 'road-friendliness' : a review

This paper is concerned with assessing the dynamic tyre forces generated by articulated heavy goods vehicles for road damaging potential. Various factors are discussed, including: (a) general testing methodologies; (b) road damage issues such as 'spatial repeatability' of dynamic tyre forces and road damage criteria; (c) vehicle response issues, such as test duration and sampling details, road roughness, testing speed, wheel-base filtering, tractor-trailer interaction, suspension maintenance and 'indirect' testing methods; and (d) implementation issues. It is concluded that the most practical method ofassessment testing would be to use a 'type approval' test to measure the vehicle's peformance when coupled to a standard trailer or tractor unit, combined with anual inspections of hydraulic damper integrity. The type approval procedure should use simple single-axle laboratory tests to estimate the parameters ofa generic mathematical model of each vehicle unit. Numerical simulations of the tractor and standard trailer (or trailer and standard tractor) should be used to determine the vehicle's response to a variety of typical road input conditions. These responses should than be assessed using realistic road damage criteria to determine an 'in-service' road damage index.
Low-order models for fast dynamical simulation of MEMS microstructures

In this paper, are describe how a few simulations of fully meshed dynamical problems can be used to construct efficient low-order models for system-level design of microstructures. We report on the use of this method to capture the measured behaviour of a pressure sensor based on the pull-in time of a beam. Results show that the reduced order model decreases simulation time by at least a factor of 37 while achieving good agreement with experimental data.
Video Games as a Testbed for Open-Ended Phenomena

Understanding and engineering open-endedness, or the indefinite generation of novelty and complexity at arbitrary scales, has long been studied by implementing nature-inspired simulations specifically designed for artificial life studies. This paper argues that video games serve as a complementary domain for research on open-endedness. In support of this claim, experiments in this paper evaluate the effects of age-based and spatial destructive events in two game domains: an interactive Game of Life and the city-building game SimCity. These games are played by a neural-network-controlled gameplay agent trying to maximize reward. Results indicate that experiments with SimCity are more likely to identify statistically significant differences in complexity as a result of applied destructive events, highlighting the utility of this game domain for studying artificial life phenomena.