Miracle-dz/UniFAR-FTUs huggingface dataset:
First novel phages from rodents with lytic activity on clinical Enterobacteriaceae strains: Initiation for phage therapy in West Africa

Bacteriophages (phages) are viruses that infect bacteria. The emergence of resistant bacteria has been reported worldwide. The use of phages to treat bacterial infection or to reduce bacteria load has become a new area of interest. The viral colonization of rodents represents an important source for a specific biotope. The objective of this study is to characterize the effect of novel phages isolated from rodents (Mastomys) using clinical Enterobacteria strains. Three novel phages were isolated from the intestinal tract of rodents. The phages belong to Myoviridae family. By using three Random Polymerase Chain Reactions (PCR), genomic characterization of the phages shows a high diversity that reveals several DNA bands. 3 virulent phages exhibited different patterns, indicating that the rodents’ phages are genetically unique and could be distinguished by typing-PCR. The virulence spectrum of the phages shows phages Mα3 and Mα7 have 50% (7/15) lytic activity on enteropathogenic and enterohemorrhagic Escherichia coli strains while phage Mα11 has 26.6% (4/15) lytic activity. These phages have no lytic activity on Salmonella strains. This study is the first to isolate novel phages from rodents with lytic activity. The candidate rodents’ phages (Mα3, Mα7, Mα11) can be used to control virulent E. coli in West Africa. Keys words: Lytic phages, rodents, Escherichia coli strains, West Africa, enteropathogenic, enterohemorrhagic.
DEVELOPMENT OF A GREEN STABILITY-INDICATING HPLC-DAD METHOD FOR THE ANALYSIS OF TILDIPIROSIN IN PHARMACEUTICAL FORMULATION

This study aimed to develop a simple stability-indicating HPLC-DAD method for the assay of Tildipirosin (TPS), which is a 16-member ring semi-synthetic macrolide, in an injectable solution. TPS is commonly used in the USA for the treatment of animalís respiratory infection. The isocratic elution was set up using the Inertsil-ODS column with the flow rate set at 0.9 mL/min and the detection wavelength of 285 nm. The column temperature was ambient and the overall runtime was 5 min. Additionally, the mobile phase was water: acetonitrile: trifluoroacetic acid (53.45 : 46.45 : 0.10, by volume). The HPLC-forced degradation stability method for TPS in Egydipiro Injectable Solution was performed in different stress conditions, i.e. light/UV, heat, acid, base, and oxidation. The results showed the linearity range was 36.00 to 108.00 μg/mL for TPS. The correlation coefficient was higher than 0.999 and the accuracy was 98.78%. The degradation percentages were presented ranging from 31.54% in the photodegradation condition to 50.10% in the base degradation. The Ecoscale score was about 82 points indicating an excellent green chromatographic technique. Therefore, the novel stability method was unique in terms of simplicity and environment-friendly aspects. The new HPLCñDAD method was the first, green, stability-indicating chromatographic method for a TPS analysis with its degradation products. However, the major drawbacks of the new chromatographic method are the incomplete identification of degradation products and the inability to propose an appropriate mechanism for drug degradation. Overall, the method can be employed to evaluate TPS stability under various storage conditions.
In vitro regeneration of Anacardium occidentale from shoot tip and basal part

The culture of cashew (Anacardiumoccidentale L.) is the main source of income for populations in northern Cote d'Ivoire, with an estimated production of 725 000 tonnes in 2017, but the average yield remains low likely due to the lack of elite planting material and hence use of unselected plant material by mostfarmers. For mass propagation of such a material, in vitro methods are necessary. Unfortunately, it is difficult to obtain surviving explants from mature plants grown in the field, whereby explants from seedlings obtained by in vitro germination are the most suitable for micropropagation of cashew.The objective of this study was to propagate under in vitroconditionselite plants of Anacardiumoccidentaleto be used as planting material. In Nangui Abrogoua University laboratory,shoot tip and basal part explants derived from vitroplants of 16-day-old were transferred onto Murashige and Skoog (MS) medium containing different concentrations of cytokinins.After one month of culture, the induced shoots were placed onto different strengths of MS medium withvarious concentrations of sucrose and auxin. The highest number of buds (9) was recorded with the basal explants on medium supplemented withThidiazuron (TDZ) at 0.01 mg/l. The highest shoots (3 cm) were obtained with these sameexplantson a medium without growth regulators. A½ MS with 60 g/l of sucrose and 5 mg/l of IBA induced the highest rooting percentage (72%) and number of roots (4 roots) in a short time (16 days).
CAF Technique in Position Recognition for Wireless Application

In military and commercial applications, locating radio frequency transmitters accurately includes global positioning, locating hostile radar systems precisely and finding the location of beacon which is sending emergency signals. Many geo location methods use Arrival Time Difference (ATD) and Arrival Frequency Difference (AFD) between two collectors which collects the signal from transmitters’ dissimilarity in two Doppler’s is same indicates that AFD is same among collectors, the ATD can be found using simple cross correlation computations. The AFD has cardinal value which prevents the use of cross correlation techniques when there is movement of transmitters with respect to collectors. To achieve Cross Ambiguity Function (CAF) parameter, an ATD and AFD must be calculated collaboratively. We adapted advanced computations approach to find out CAF efficiently. Using advanced computations, the modified CAF mapping is projected and also shown geo location of the beacon emitting signals can be done by using CAF mapping method. Analysis results discussion is provided to verify simulation.
A Multi-Featured Actor-Critic Relay Selection Scheme for Large-Scale Energy Harvesting WSNs

In this letter, we focus on the design of a relay selection scheme in large-scale energy-harvesting wireless sensor networks. Considering the dynamic nature required for practical networks, multiple features, including queuing state, energy level, channel quality, and location, are involved in modeling. By introducing reinforcement learning techniques, a novel relay selection scheme based on an actor-critic algorithm with linear function approximation is proposed to improve network reliability while also taking into account the transmission delay and energy efficiency. The proposed scheme can be implemented independently in each source to maximize the data delivery ratio. Such a distributed scheme is more stable and scalable than centralized structures. The Lagrangian formula is applied to satisfy the constraints on hops and energy efficiency. Compared with traditional timer-based and Q-learning-based schemes, our simulation results show that our proposed scheme achieves good performance in terms of network reliability and obtains higher energy efficiency and lower transmission delay.
Solubility phase transition behavior of gold nanoparticles in colloidal solution

Nano-size materials are new materials in an intermediate state between the bulk and atomic or molecular states. Nanoparticles in colloidal solution and their assemblies have the great attention of researchers to investigate the novel fundamental properties and numerous applications. In this dissertation, we investigated the solubility phase transition behavior of gold nanoparticles in colloidal solution. We used the nearly monodisperse gold nanoparticles synthesized by either the inverse micelle or the solvated metal atom dispersion methods followed by digestive ripening. The gold nanoparticles were ligated with alkyl chains, which were dodecanethiol, decanethiol, or octanethiol for individual samples. They dispersed in toluene or t-butyl toluene like large molecules at room temperature. In analogy to molecular solutions, the colloidal solution had thermally reversible phase transitions between a dissolved phase of dispersed single nanoparticles and dispersed-aggregation co-existing phase. A more polar solvent, 2-butanone, was added to the colloidal solution for changing the solubility of gold nanoparticles and adjusting the phase transition temperatures to accessible temperatures. Superclusters formed by the nanoparticles when the colloidal solutions were quenched from a one-phase regime at high temperature to a two-phase regime at low temperature. Solubility phase diagrams were obtained for gold nanoparticles with different ligands in the mixtures of different ratios of 2-butanone and toluene or t-butyl toluene. The explanation from classical ideal solution theory gave the fusion enthalpy of superclusters. Temperature quenches from the one-phase to the two-phase regime yielded superclusters of the nanoparticle solid phase with sizes that depended on the quench depth. Classical nucleation theory was used to describe these sizes using a relative small value of the surface tension for the nanoparticle solid phase. This value is consistent with molecule size scaling of the surface tension. In total these results show that the solubility behavior of nanoparticles in colloidal solution is similar to the behavior in molecular solutions. SOLUBILITY PHASE TRANSITION BEHAVIOR OF GOLD NANOPARTICLES IN COLLOIDAL SOLUTION by
Can MED-RT Summarization Support Missing Adverse Drug Reactions Discovery?

Adverse drug reactions (ADRs) cause many hospitalizations and cases of mortality every year. A comprehensive list of potential ADRs for each drug is essential for assessing patients’ risks of ADRs when prescribing a medication. The Medication Reference Terminology (MED-RT) is a new drug terminology developed by the Veterans Health Administration. In this study, we examine whether the summarization of the chemical ingredients in MED-RT can support identifying knowledge gaps in lists of potential ADRs. MED-RT is a virtual terminology, which does not maintain drug and chemical ingredient concepts. Instead, it refers to external standard terminologies. In this study, we develop a new Summarization Network called Aggregate Virtual Ingredient Summarization Network (AVISN) for summarization of the MED-RT virtual Chemical Ingredients hierarchy. We demonstrate the use of the AVISN for discovering potential gaps for conditions associated with ADRs in First Databank’s Drug-Disease Contraindications knowledge base.
ANTIOXIDANT ACTIVITY AND TOTAL PHENOL CONTENT OF DIFFERENT PLANT PARTS OF LEBANESE ANNONA SQUAMOSA LINN.

Objective: The aim of this work was to characterize the antioxidant properties and to evaluate the total phenol content of leaves, bark, pericarp, and pulp extracts of Lebanese Annona squamosa Linn. (A. squamosa),, as well as a total screening of secondary metabolites present in the various plant parts studied. Methods: Two solvent systems were used for extraction: ethanol 80 % and methanol 80 %. The antioxidant activity of different extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The Total Phenol Content (TPC) of the different plant parts are determined and compared via Folin-Ciocalteu method. The results were presented as the mean of three separate experiments and error bars were used to illustrate standard deviation. Results: The phenolic content was found to be highest in the A. squamosa leaves methanolic and ethanolic extracts (117.2 mg and 112.92 gallic acid extract/g, respectively). The results showed that A. squamosa leaves methanolic and ethanolic extracts display the highest antioxidant activities than the bark, pulp and pericarp extracts, with half-maximal inhibitory concentration values 13.61 and 15.97 μg. ml-1 respectively. Ethanol 80 % and methanol 80 % were found to be efficient for the extraction of phenolic compounds. Conclusion: Results of this study indicate the presence of promising compounds in Lebanese A. squamosa that are able to act as antioxidants and free radical scavengers.
Sociological analysis of Montenegrin teachers’ digital capital

Partindo do conceito de capital digital nas Ciencias Sociais, este artigo apresenta as principais conclusoes do inquerito nacionalmente representativo do “Global Kids Online” sobre as competencias e praticas digitais de professores do ensino basico e secundario, realizado em Montenegro com o apoio da Unicef, em 2018. O capital digital, como qualquer outra forma de capital na perspetiva de Bourdieu, apenas tem validade sociologica em correlacao com outras formas de capital, como a economica, cultural e social, num contexto limitado e de acordo com uma abordagem multidimensional que vai de uma perspetiva macro a uma microssocial (Pandolfini, 2016). Este artigo identifica e discute tres perspetivas do capital digital – macro, meso e micro social – e os seus recursos materiais (tecnologias, servicos digitais e experiencias escolares com dispositivos) e nao materiais (competencias digitais). A analise dos dados do estudo montenegrino, de acordo com esta perspetiva, mostra que a pratica diaria de utilizacao da tecnologia digital nas salas de aula parece ser marginal, embora a maioria dos professores tenha acesso a internet nas escolas. Atualmente, a maioria dos professores utiliza a internet na escola principalmente para verificar informacao online. Geralmente, as suas competencias digitais nao sao avancadas: em media, as competencias sociais e operacionais sao as mais desenvolvidas, enquanto as competencias criativas estao menos desenvolvidas. Portanto, para apoiar o desenvolvimento da literacia mediatica nas criancas atraves da educacao formal, e necessario realizar mais investimentos para reforcar as competencias digitais dos professores. O estudo mostra ainda que a procura por cursos de pedagogia digital ja existe entre a maioria dos professores. Por outras palavras, o estudo montenegrino aponta para a necessidade de investir mais na educacao e na experimentacao associadas as perspetivas meso e microssocial do capital digital.
Lithology‐dependent seismic anisotropic amplitude variation with offset correction in transversely isotropic media

Analysis of amplitude variation with offset is an essential step for reservoir characterization. For an accurate reservoir characterization, the amplitude obtained with an isotropic assumption of the reservoir must be corrected for the anisotropic effects. The objective is seismic anisotropic amplitude correction in an effective medium, and, to this end, values and signs of anisotropic parameter differences (Δδ and Δε) across the reflection interfaces are needed. These parameters can be identified by seismic and well log data. A new technique for anisotropic amplitude correction was developed to modify amplitude changes in seismic data in transversely isotropic media with a vertical axis of symmetry. The results show that characteristics of pre‐stack seismic data, that is, amplitude variation with offset gradient, can be potentially related to the sign of anisotropic parameter differences (Δδ and Δε) between two layers of the reflection boundary. The proposed methodology is designed to attain a proper fit between modelled and observed amplitude variation with offset responses, after anisotropic correction, for all possible lithofacies at the reservoir boundary. We first estimate anisotropic parameters, that is, δ and ε, away from the wells through Backus averaging of elastic properties resulted from the first pass of isotropic pre‐stack seismic inversion, on input data with no amplitude correction. Next, we estimate the anisotropic parameter differences at reflection interfaces (values and signs of Δδ and Δε). We then generate seismic angle gather data after anisotropic amplitude correction using Rüger's equation for the P‐P reflection coefficient. The second pass of isotropic pre‐stack seismic inversion is then performed on the amplitude‐corrected data, and elastic properties are estimated. Final outcome demonstrates how introduced methodology helps to reduce the uncertainty of elastic property prediction. Pre‐stack seismic inversion on amplitude‐corrected seismic data results in more accurate elastic property prediction than what can be obtained from non‐corrected data. Moreover, a new anisotropy attribute (ν) is presented for improvement of lithology identification.
An Enhanced Adaptive Perturb and Observe Technique for Efficient Maximum Power Point Tracking Under Partial Shading Conditions

In this paper, we propose enhanced adaptive step size Perturb and Observe (P&O) maximum power point tracking (MPPT) with properly organized comparison sequences which lead to achieving the actual maximum power point (MPP) effectively in the presence of partial shading conditions, taking into account the optimization of all aspects of high-performance MPPT to be novel, simpler, fast, and accurate, with the best efficiency reaching up to almost 100%. In this study, the proposed algorithm, along with a boost converter, was designed and simulated in MATLAB/Simulink to validate the performance of the suggested technique. Four different levels of partial shading conditions were considered for system examination: weak, moderate, and two different levels of strong shading. Each case was applied separately first and then combined in a sequence arrangement to provide robust and comprehensive testing which can provide a guaranteed assessment of the proposed algorithm. The performance of the suggested technique is discussed and compared with that of conventional P&O and conventional incremental conductance (IC) MPPT techniques. The failure of the conventional techniques to work efficiently in the presence of partial shading conditions was observed from the simulation results. Meanwhile, the success of the proposed technique and its high performance were clearly confirmed under partial shading conditions with no increase in complexity or convergence time.
EVALUATION AND TAILORING OF GLOBAL GEOPOTENTIAL MODELS IN THE DETERMINATION OF GRAVITY FIELD IN SERBIA

Within this paper, we evaluated the quality of three Global Geopotential Models entitled: EGM96,EGM2008, and GOCO05c. The models were evaluated by using 1001 terrestrial discrete values ofheight anomalies determined by Global Navigation Satellite Systems and normal heights, which weconsidered to be true values within this research. In addition to the quality evaluation, we tailoredthe models by using more than 80000 free air anomalies. The results obtained from the evaluationand tailoring indicate that by using the GOCO05c it is possible to determine a set of anomaly heightsacross Serbia, which are in agreement with terrestrial values with an average value of -7 cm, thestandard deviation of ±9 cm and with the range of 44 cm.
Multi‐metal doped high capacity and stable Prussian blue analogue for sodium ion batteries

Prussian blue analogues (PBA) are attractive positive electrode for sodium ion batteries due to their large interstitial sites and three‐dimensional porous framework. However, they often suffer from irreversible phase transition upon charging and discharging, leading to fast capacity decay. In consideration that redox couples of Co2+/Co3+, Ni2+/Ni3+ and Fe2+/Fe3+ guarantee high capacity (~150 mA h g−1), and Cu serves as a pillar to hold the PBA framework, here we synthesized a series of Cu, Co and Ni co‐doped PBA, displaying high capacity and stable cycling performance with >98% capacity retention after 50 cycles.
Less Sex, but More Sexual Diversity: Changes in Sexual Behavior during the COVID-19 Coronavirus Pandemic

Abstract Recreational sex is a popular form of leisure that has been redefined by the COVID-19 (coronavirus) pandemic. “Social distancing” rules have imposed limits on sex for leisure while also creating new opportunities. We discuss results from an online survey of 1,559 adults who were asked about the pandemic’s impact on their intimate lives. While nearly half of the sample reported a decline in their sex life, one in five participants reported expanding their sexual repertoire by incorporating new activities. Common additions included sexting, trying new sexual positions, and sharing sexual fantasies. Being younger, living alone, and feeling stressed and lonely were linked to trying new things. Participants making new additions were three times more likely to report improvements in their sex life. Even in the face of drastic changes to daily life, many adults are adapting their sexual lives in creative ways.
Presence of aprX gene in Pseudomonas spp. from refrigerated raw milk and their proteolytic ability

This study aimed to determine the frequency of Pseudomonas fluorescens, P. putida, and P. aeruginosa in refrigerated raw milk; their proteolytic potential; and your association with the aprX gene. Of the 173 isolates confirmed as belonging to Pseudomonas spp. , 37% were P. fluorescens, 25.4% P. putida, and none belongs to P. aeruginosa. The aprX gene was distributed proportionally between P. putida (68%) and P. fluorescens (75%), but it was not associated with low or high proteolytic potential in both species. P. putida (16) and P. fluorescens (14) isolates with no aprX gene identified also had proteolytic potential. Considering the synthesis of proteases other than AprX by the isolates under study, we concluded that P. fluorescens and P. putida represented 62.4% of the Pseudomonas genus, with high probability of having the aprX gene and proteolytic potential. However, there was no association between the deteriorating potential with the presence of the aprX gene.
Development of a Noise Generation Device with Polarization Control

The development of polarization processing methods in radar and radio communication systems has led to the need of creation special noise generators with control of the polarization of the generated signal to research radio engineering systems (RES) with full polarization processing and to block the operation of the RES at high-security facilities. In this article, various schemes of noise generation devices with control of the degree of polarization and orientation angle are proposed. The influence of non-identity of radio paths on the generated signal is estimated. Methods for calculating the parameters of the generated electromagnetic field and experimental evaluation of the parameters are described.
The effectiveness method (ε-NTU) to analyze the thermal performance of the flat tube multi-louvered finned radiator with silver nanoparticles suspension in ethylene Glycol

The analytical efficacy method (ε-NTU) is applied to analyze the thermal performance of the radiator with MultiLouvered flat-tube fins. The nanofluid is composed of a suspension of silver nanoparticles in ethylene glycol. The rate of heat transfer, efficiency, and outlet temperatures of the nanofluid and air are determined and presented in graphical form. The volumetric fraction of the nanoparticles suspended in ethylene glycol, the variations in the airflow rate and the nanofluid flow rate is the main parameters used in the analysis. The analysis showed promising results since it is possible to save on the use of the compact heat exchanger considered, reducing costs and storage space for the refrigerant.