Developmental hematopoiesis : methods and protocols

Part I. Genetic Approaches Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse Hanna K. A. Mikkola and Stuart H. Orkin Inducible Transgene Expression in Mouse ES Cells David T. Ting, Michael Kyba, and George Q. Daley Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells Hans-Willem Snoeck Part II. Analysis of Cell Fate Outcomes in Transplantation Models Flow Cytometric Analysis of Hematopoietic Development Edward F. Srour and Mervin C. Yoder Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flake Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice Scott A. Johnson and Mervin C. Yoder Part III. Animal Models Drosophila Hemocyte Development During Drosophila Embryogenesis Robert A. Schulz and Nancy Fossett Xenopus Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos Maggie Walmsley, Aldo Ciau-Uitz, and Roger Patient Fate Mapping Hematopoietic Lineages in the Xenopus Embryo Mary Constance Lane and Michael D. Sheets Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain) Yumi Izutsu and Mitsugu Maeno Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo James B. Turpen Zebrafish Analysis of Hematopoietic Development in the Zebrafish Noelle N. Paffett-Lugassy and Leonard I. Zon Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos Philippe Herbomel and Jean-Pierre Levraud. Chick In Vivo Methods to Analyze CellOrigins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo Francoise Dieterlen-Lievre, Sophie Creuzet, and Josselyne Salaun Mouse Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development Margaret H. Baron and Deanna Mohn Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo Catherine Robin and Elaine Dzierzak Hematopoietic Stem Cell Development During Mouse Embryogenesis Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godin Analysis of Hematopoietic Progenitors in the Mouse Embryo James Palis and Anne Koniski In Vivo and In Vitro Assays of Thymic Organogenesis Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburn Retroviral Transduction in Fetal Thymic Organ Culture Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors Helmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Mullner, and Hartmut Beug A New Clonal Assay System for Lymphoid and Myeloid Lineages Hiroshi Kawamoto and Yoshimoto Katsura Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang Analysis of the Vascular Potential of Hematopoietic Stem Cells Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scott Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture Elizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinson Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation Matthias