Miracle-dz/UniFAR-FTUs huggingface dataset:
Heart Rate Change during Aquatic Exercise in Small, Medium and Large Healthy Dogs

บทคดยอ ทำการศกษาการเปลยนแปลงอตราการเตนของหวใจของสนขทสขภาพดในขณะวายนำ โดยแบงการศกษาเปน 2 การทดลอง การทดลองท 1 เกบขอมลจาก สนขเพศผทสขภาพด จำนวน 21 ตว ทใหวายนำทกๆ 2 วน ตดตอกน 8 ครงๆ ละ 10 นาท และทำการวดอตราการเตนของหวใจขณะวายนำทกนาท การทดลองท 2 เกบขอมลจาก สนขสขภาพด จำนวน 134 ตว ซงแบงเปน 3 กลม ไดแก สนขขนาดเลก(41 ตว) ขนาดกลาง (51 ตว) และขนาดใหญ (42 ตว) ทำการวดอตราการเตนของหวใจทกนาท ตดตอกน 34 นาท หลงการวายนำครงท 5การทดลองท 1 พบวา อตราการเตนของหวใจของสนขขณะวายนำ ใน 4 ครงแรกสงกวาในครงท 5 ถง 8 (p<0.05) การทดลองท 2 พบวาอตราการเตนของหวใจของสนขขนาดเลก กลาง และใหญ มความแตกตางกนทางสถต (p<0.05) และไมพบความสมพนธระหวางนำหนกรางกาย และอายกบอตราการเตนของหวใจ นอกจากนคาอตราการเตนของหวใจทบนทกไดจากการทดลอง สามารถนำมาสรางเปนสมการหาอตราการเตนของหวใจของสนขแตละขนาด (เลก กลาง และใหญ) ขณะวายนำ จากการศกษา แนะนำใหสนขวายนำตดตอกน 15 ถง 30นาท ขนอยกบขนาดของสนขและผควบคมการออกกำลงกายตองคอยสงเกตอาการของสนขแตละตวเพอปองกนการออกกำลงกายมากเกนความสามารถ คำสำคญ: สนข อตราการเตนของหวใจ วายนำ Abstract This study was divided into two experiments. For the first experiment, heart rates during exercise of 21healthy male dogs were recorded. The animals were brought to swim every 2 days, 8 times in all, for 10 minutes.Heart rates were measured every minute using a pulse watch. For the second experiment, 134 healthy adult dogswere categorized into three groups: small (41), medium (51) and large breed (42). Their heart rates were measuredevery minute for 34 minutes after the 5th swimming. In the first experiment, the heart rates during the 1st–4thswimming were significantly higher (p<0.05) than during the 5th 8th swimming. In the second experiment, the heartrates were significantly different (p<0.05) between small, medium and large dogs. No correlations were foundbetween weight, age, and heart rate. From the results of the second experiment, we were able to formulate anequation for predictable heart rate of each group of dogs (small, medium and large dogs). From the results, werecommend that the limits on the length of time spent for aquatic exercise should be 15–30 minutes, depending on thebreed (size) of dog. Moreover, there should be trainers observing each dog to prevent over-exercise. Keywords: dog, heart rate, swimming
Association of Personal Protective Equipment Use with Successful Protection Against COVID-19 Infection Among Health Care Workers

The coronavirus disease 2019 (COVID-19) pandemic has posed a major challenge for protecting health care workers (HCWs) against the infection. Use of personal protective equipment (PPE) in health care workplace is recommended as a high priority. In order to investigate the relationship between PPE use and the number of COVID-19 cases among HCWs, we conducted a molecular epidemiological study among 142 HCWs who were dispatched from Hefei to work in Wuhan and 284 HCWs who remained in Hefei, China; both provided care for patients with COVID-19. Nucleic acid testing and SARS-CoV-2 specific antibody (IgM, IgG, IgA) detection were performed to confirm SARS-CoV-2 infection among those HCWs. We also extracted publicly released data on daily number of COVID-19 cases among HCWs, daily number of HCWs who were dispatched to Hubei province since January 24, and daily production of PPE in China and daily demand and supply of PPE in Hubei province. Our laboratory testing confirmed that none of the 142 HCWs who were dispatched to work in Wuhan and 284 HCWs who remained in Hefei were infected by SARS-CoV-2. Consistent with these findings, as of April 15, 2020, none of the 42,600 HCWs who were successively dispatched to Hubei province since January 24, 2020 was reported to have COVID-19. These HCWs were provided with adequate supply of PPE as committed by their original institutions or provinces. In contrast, during the early phase of COVID-19 epidemic in Hubei province, a substantial shortage of PPE and an increasing number of COVID-19 infection among HCWs were reported. With the continuing increase in domestic production of PPE in China, the PPE supply started to meet and then exceed the demand. This coincided with a subsequent reduction in the number of reported COVID-19 cases among HCWs. In conclusion, our findings indicate that COVID-19 infection among HCWs could be completely prevented. Appropriate and adequate PPE might play a crucial role in protecting HCWs against COVID-19 infection.
Particle Based Model for Airborne Disease Transmission

Prior literature documents cases of airborne infectious disease transmission at distances ranging from [≥] 2 m to inter-continental in scale. Physics- and biology- based models describe the key aspects of these airborne disease transmission events, but important gaps remain. This report extends current approaches by developing a new, single-particle based theory that (a) assesses the likelihood of rare airborne infections (where individuals inhale either one or no infectious particles) and (b) explicitly accounts for the variability in airborne exposures and population susceptibilities within a geographic region of interest. For these hazards, airborne particle fate and transport is independent of particulate concentration, and so results for complex releases can be determined from the results of many single-particle releases. This work is intended to provide context for both (a) the initial stages of a disease outbreak and (b) larger scale ([≥] 2 m) disease spread, including distant disease "sparks" (low probability, unexpected disease transmission events that infect remote, susceptible populations). The physics of airborne particulate dispersion inherently constrains airborne disease transmission. As such, this work suggests results that, a priori, may be applicable to many airborne diseases. Model Predictions: i. Modeling predictions of the single-particle transmission kernel suggest that outdoor airborne disease transmission events may occur episodically as the infection probabilities can vary over many orders of magnitude depending on the distance downwind; specific virus, prion, or microorganism; and meteorological conditions. ii. Model results suggest that, under the right conditions, an indoor infected person could spread disease to a similar, or greater, number of people downwind than in the building they occupy. However, the downwind, per-person infection probability is predicted to be lower than the within-building, per-person infection probability. This finding is limited to airborne transmission considerations. iii. This work suggests a new relative disease probability metric for airborne transmitted diseases. This metric, which is distinct from the traditional relative risk metric, is applicable when the rate at which the infectious agent losses infectivity in the atmosphere is [lsim] 1 h-1.
Rumen Protozoa Play a Significant Role in Fungal Predation and Plant Carbohydrate Breakdown

The rumen protozoa, alongside fungi, comprise the eukaryotic portion of the rumen microbiome. Rumen protozoa may account for up to 50% of biomass, yet their role in this ecosystem remains unclear. Early experiments inferred a role in carbohydrate and protein metabolism, but due to their close association with bacteria, definitively attributing these functions to the protozoa was challenging. The advent of ‘omic technologies has created opportunities to broaden our understanding of the rumen protozoa. This study aimed to utilize these methods to further our understanding of the role that protozoa play in the rumen in terms of their metabolic capacities, and in doing so, contribute valuable sequence data to reduce the chance of mis or under-representation of the rumen protozoa in meta’omic datasets. Rumen protozoa were isolated and purified using glucose-based sedimentation and differential centrifugation, extracted RNA was Poly(A) fraction enriched and DNase treated before use in a phage-based, cDNA metatranscriptomic library. Biochemical activity testing of the phage library showed 6 putatively positive plaques in response to carboxymethyl cellulose agar (indicative of cellulose activity), and no positive results for tributyrin (indicative of esterase/lipase activity) or egg yolk agar (indicative of proteolysis). Direct sequencing of the cDNA was also conducted using the Illumina HiSeq 2500. The metatranscriptome identified a wealth of carbohydrate-active enzymes which accounted for 8% of total reads. The most highly expressed carbohydrate-active enzymes were glycosyl hydrolases 5 and 11, polysaccharide lyases and deacetylases, xylanases and enzymes active against pectin, mannan and chitin; the latter likely used to digest rumen fungi which contain a chitin-rich cell membrane. Codon usage analysis of expressed genes also showed evidence of horizontal gene transfer, suggesting that many of these enzymes were acquired from the rumen bacteria in an evolutionary response to the carbohydrate-rich environment of the rumen. This study provides evidence of the significant contribution that the protozoa make to carbohydrate breakdown in the rumen, potentially using horizontally acquired genes, and highlights their predatory capacity.
Delineation and Modulation of the Natural Killer Cell Transcriptome in Rhesus Macaques During ZIKV and SIV Infections

Natural killer (NK) cells are crucial regulators of antiviral and anti-tumor immune responses. Although in humans some NK cell transcriptional programs are relatively well-established, NK cell transcriptional networks in non-human primates (NHP) remain poorly delineated. Here we performed RNA-Seq experiments using purified NK cells from experimentally naïve rhesus macaques, providing the first transcriptional characterization of pure NK cells in any NHP species. This novel NK cell transcriptomic signature (NK RMtsig) overlaps with published human NK signatures, allowing us to identify new key signaling and transcription factor networks underlying NK cell function. Finally, we show that applying NK RMtsig to an unrelated rhesus macaque cohort infected with SIVmac251 or ZIKV can sensitively detect NK cell repertoire perturbations, thus confirming applicability of this approach. In sum, we propose this NHP NK cell signature will serve as a useful resource for future studies involving infection, disease or treatment modalities in NHP.
Network Analysis of Cattle Movement in Mato Grosso Do Sul (Brazil) and Implications for Foot-and-Mouth Disease

Foot-and mouth disease (FMD) is an animal disease that generates many economic impacts and sanctions on the international market. In 2018, Brazil, the world's largest beef exporter, had the recognition by World Organization for Animal Health (OIE) as a country free of FMD with vaccination and proposed to withdraw FMD vaccination throughout the country, based on a 10-year schedule, beginning in 2019. Therefore, Brazil needs studies to help the decision-making process, particularly regarding the availability of resources for strengthening of official animal health services. The state of Mato Grosso do Sul (MS) was chosen to be analyzed for three reasons: the size of its herd, the economic importance of its livestock and its location—which lies on the border with Paraguay and Bolivia. The current study adopted the Social Network Analysis and performed an exploratory analysis of cattle movement in MS. The most central municipalities in the networks were identified and they can be seen as crucial in strategies to monitor animal movement and to control outbreaks. The cattle movement networks demonstrated to be strongly connected, implying a high-speed potential FMD diffusion, in case of reintroduction. In a second stage, we performed an exploratory analysis of animal movement within the state, assuming distinct points in time for the identification of animal origin. The results of the analysis underlined the need and relevance of investing in animal control, sanitary education for producers and equipment and technologies to assist in the early detection, diagnosis, and eradication of outbreaks in a fast and efficient manner, preventing a possible outbreak from spreading to other regions.
RAGE Silencing Ameliorates Neuroinflammation by Inhibition of p38-NF-κB Signaling Pathway in Mouse Model of Parkinson’s Disease

Accumulating evidence suggested that neuroinflammation played a crucial role in dopaminergic neuronal death in Parkinson’s disease (PD). The receptor for advanced glycation end products (RAGE), a multi-ligand receptor of the immunoglobulin superfamily, has been proposed as a key molecule in the onset and sustainment of the inflammatory response. Engagement of RAGE contributed to neuroinflammation by upregulating nuclear factor-κB (NF-κB) as well as cytokines. The aim of the present study was to investigate the expression of RAGE in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice and elucidate the RAGE signal pathway involved in the inflammation. Results showed that RAGE protein and pro-inflammatory cytokines cyclooxygenase type 2 (COX-2) were upregulated in MPTP-treated mice. Further experiments showed that RAGE ablation inhibited phosphorylation of IκB and p38 and protected nigral dopaminergic neurons against cell death in the substantia nigra (SN). These results suggested that RAGE participated in the pathogenesis of PD by neuroinflammation and p38MAPK-NFκB signal pathway may be involved in the process. Moreover, interfering with RAGE signaling pathway may be a reasonable therapeutic option in slowing PD development and progression.
Exit Option Induced by Win-Stay-Lose-Leave Rule Provides Another Route to Solve the Social Dilemma in Structured Populations

Theoretical studies have shown that cooperation can be promoted by the so-called “network reciprocity,” where cooperation can coexist with defection via the compact cooperative clusters. However, such studies often assume that players have no chance to exit from the game even if the situation is extremely bad for them, which is in sharp contrast with the real-life situations. Here, we relax this assumption by giving players the right to choose between one of two states, active (participate in the game) and inactive (exit from the game). We define this assumption as win-stay-lose-leave rule. This new rule motivates a winner whose payoff is larger than the average payoff of its neighbors to stay in its current state, thus retains its current advantage compared to its neighbors. Conversely, a loser is pushed to leave from its current state which in turn increase its chance to obtain a higher payoff while in inactive state. Specifically, we incorporate exit cost into consideration by assuming that anyone who decides to exit from the game must pay a cost γ. Extensive numerical simulation show that if the exit cost is intermediate (neither too high or too small), a full cooperation plateau is achieved, where cooperation evolves with the support of enhanced network reciprocity. In fact, inactive players can only exist at the boundary of cooperative clusters, which creates a crucial buffer area for the endangered cooperators. As a consequence, the joint effect of this protective film composed of inactive players and cooperative clusters forms the foundation of enhanced network reciprocity.
“Do Good, Have Good”: A Serial Mediation Analysis of CSR with Customers’ Outcomes

Corporate social responsibility (CSR) is envisaged to offer several outcomes and while customer-specific consequences are unclear or have not obtained due attention, there is a dearth of literature that focuses on perceptual, attitudinal and behavioral outcomes in tandem. Against this backdrop, this study entails the investigation of perceptual (service quality), attitudinal (affective commitment) and behavioral (customer citizenship behavior) outcomes of CSR through a serial mediation mechanism. A total of 994 responses obtained from fast-food restaurants, highlight the fact that CSR influences service quality, affective commitment, and customer citizenship behavior. Moreover, it is witnessed that CSR influences customer citizenship behavior indirectly as well, as the serial mediation mechanism is also proved. The practical and theoretical usefulness of the study is also provided at the end.
Light-Dependent Activation of the GCN2 Kinase Under Cold and Salt Stress Is Mediated by the Photosynthetic Status of the Chloroplast

Regulation of cytosolic mRNA translation is a key node for rapid adaptation to environmental stress conditions. In yeast and animals, phosphorylation of the α-subunit of eukaryotic translation initiation factor eIF2 is the most thoroughly characterized event in regulating global translation under stress. In plants, the GCN2 kinase (General Control Non-derepressible-2) is the only known kinase for eIF2α. GCN2 is activated under a variety of stresses including reactive oxygen species. Here we provide new evidence that the GCN2 kinase in Arabidopsis is also activated rapidly and in a light dependent manner by cold and salt treatments. These treatments alone did not repress global mRNA ribosome loading in a major way. The activation of GCN2 was attenuated by inhibitors of photosynthesis and antioxidants, suggesting that it is gated by the redox poise or the reactive oxygen status of the chloroplast. In keeping with these results, gcn2 mutant seedlings were more sensitive than wild type to both cold and salt in a root elongation assay. These data suggest that cold and salt stress may both affect the status of the cytosolic translation apparatus via the conserved GCN2-eIF2α module. The potential role of the GCN2 kinase pathway in the global repression of translation under abiotic stress will be discussed.
The Potential Role of Marine Protein Hydrolyzates in Elevating Nutritive Values of Diets for Largemouth Bass, Micropterus salmoides

The study was conducted to explore the improvement function of marine protein hydrolyzates on nutritive values of diets for largemouth bass. The diet with no inclusion of marine protein hydrolyzate was considered as the control (NC), and four other diets were formulated with fish soluble (FS), squid paste (SqP), shrimp paste (ShP), or a mixture of FS, SqP, and ShP (Mix). Triplicate tanks of fish were fed to apparent satiation twice daily for 81 days. Results showed that the growth performance was elevated due to the inclusion of marine protein hydrolyzates along with significantly elevated feed intake and protein digestibility. Thereinto, the inclusion of FS and ShP significantly improved the growth performance compared to NC. The supplementation of marine protein hydrolyzates elevated the protein content and lysozyme activity of serum, but significantly decreased the activity of liver alanine transaminase and aspartate transaminase. The gene expression analysis revealed that marine protein hydrolyzate inclusion up-regulated the expression of insulin-like growth factor I (IGF-I) with increased expression of TOR pathway-related genes including protein kinase B (AKT) 1 and ribosomal protein S6. However, the expression of activating transcription factor 4 (ATF4) and regulated in development and DNA damage responses 1 (REDD1) involved in the amino acids response (AAR) pathway was depressed with the addition of marine protein hydrolyzates. The activation of the TOR pathway and depression of the AAR pathway may be beneficial for the improved performance of fish. In the above, the marine protein hydrolyzate, especially FS and ShP, can elevate nutritive values of diets for largemouth bass.
Comparative Transcriptome and Proteome Analysis of Heat Acclimation in Predatory Mite Neoseiulus barkeri

In our previous study, we reported a high temperature adapted strain (HTAS) of the predatory mite Neoseiulus barkeri was artificially selected via a long-term heat acclimation (35°C) and frequent heat hardenings. To understand the molecular basis of heat acclimation, ‘omics’ analyses were performed to compare the differences between HTAS female adults to conventional strain (CS) at transcriptional and translational levels. We obtained a total of 5,374 differentially expressed genes and 500 differentially expressed proteins. Among them, 119 transcripts had concurrent transcription and translation profiles. It’s conserved that some processes, such as high expression of heat shock protein (HSP) genes, involved in heat tolerance of transcriptome analyses, while many protective enzymes including glutathione S-transferase, superoxide dismutase, peroxidase, and cytochrome P450 displayed down-regulated expression. KEGG analysis mapped 4,979 and 348 differentially expressed genes and proteins, to 299 and 253 pathways, respectively. The mitogen-activated protein kinases (MAPK) signaling pathway may provide new insights for the investigation of the molecular mechanisms of heat tolerance. Correlation enriched pathways indicated that there were four pathways associated with heat acclimation involving in energy metabolism and immunity. In addition, the expression patterns of ten randomly selected genes including HSP were consistent with the transcriptome results obtained through quantitative real-time PCR. Comparisons between transcriptome and proteome results indicated the upregulation of HSPs and genes participated in ATP production, immunity and energy metabolism process. A majority of antioxidant-related genes and detoxication-related genes were down-regulated suggesting a fitness cost of heat acclimation. Our results demonstrated that heat tolerance during a long-time acclimation of N. barkeri is a fairly complicated process of physiological regulations. These findings also contribute to a better understanding of the mechanisms of thermal responses of phytoseiid mites which could provide useful information for biological control through natural enemies.
Gender Agreement Attraction in Greek Comprehension

This work explores gender agreement attraction in comprehension. Attraction occurs when an agreement error (such as, “the key to the cabinets are rusty”) goes unnoticed, leading to the illusion of grammaticality due to a mismatch between the value of the head and the value of a local intervening phase (attractor). According to retrieval accounts, these errors occur during cue retrieval from memory and predict illusions of grammaticality. Alternatively, representational accounts predict that the errors occur due to the faulty representation of certain features, thus, illusions of ungrammaticality are also expected. In four experiments we explore: (a) whether gender agreement attraction occurs in Greek and the strategy/-ies employed, (b) the role of the agreement target, (c) the timing of gender agreement attraction, (d) the role of phonological matching between the nominal inflectional morphemes of the attractor and the agreement target, and (e) participants’ sensitivity to agreement when there is no conflict from the attractor. In all four experiments, the grammaticality of the sentence and the attractor value (match or mismatch with the head) and also the phonological matching between the attractor and the agreement target in ungrammatical sentences were manipulated. The agreement target was either an adjectival predicate or an object-clitic and the gender value of the head was feminine or neuter. Attraction was found in all measures during the time-course of adjectival predicates (Experiment 1) and object-clitics (Experiment 2), and in timed (Experiment 3), and untimed (Experiment 4) judgments. Even more, both gender values showed attraction and the results mainly suggest that participants experience illusions of grammaticality, confirming retrieval accounts. Phonological matching did not modulate attraction in any of the experiments, suggesting that the similarity in the morphophonological realization between the agreement target and the attractor does not increase attraction. Furthermore, participants were sensitive to gender agreement violations in the absence of gender mismatch between the head and the attractor, suggesting that they respect agreement rules and have both neuter and feminine available in their feature content repertoire, although with some tendency in favor of neuter in feminine agreement contexts. The impact of these findings is discussed within the concept of attraction and sensitivity to agreement violations.