Miracle-dz/UniFAR-FTUs huggingface dataset:
Real-Time Queue Length Detection with Roadside LiDAR Data

Real-time queue length information is an important input for many traffic applications. This paper presents a novel method for real-time queue length detection with roadside LiDAR data. Vehicles on the road were continuously tracked with the LiDAR data processing procedures (including background filtering, point clustering, object classification, lane identification and object association). A detailed method to identify the vehicle at the end of the queue considering the occlusion issue and package loss issue was documented in this study. The proposed method can provide real-time queue length information. The performance of the proposed queue length detection method was evaluated with the ground-truth data collected from three sites in Reno, Nevada. Results show the proposed method can achieve an average of 98% accuracy at the six investigated sites. The errors in the queue length detection were also diagnosed.
Eco-Physiological Traits Related to Recovery from Complete Submergence in the Model Legume Lotus japonicus

Submergence is a severe form of stress for most plants. Lotus japonicus is a model legume with potential use in assisting breeding programs of closely related forage Lotus species. Twelve L. japonicus genotypes (10 recombinant inbred lines (RILs) and 2 parental accessions) with different constitutive shoot to root dry mass ratios (S:R) were subjected to 7 days of submergence in clear water and allowed to recover for two weeks post-submergence; a set of non-submerged plants served as controls. Relative growth rate (RGR) was used to indicate the recovery ability of the plants. Leaf relative water content (RWC), stomatal conductance (gs), greenness of basal and apical leaves, and chlorophyll fluorescence (Fv/Fm, as a measure of photoinhibition) were monitored during recovery, and relationships among these variables and RGR were explored across genotypes. The main results showed (i) variation in recovery ability (RGR) from short-term complete submergence among genotypes, (ii) a trade-off between growth during vs. after the stress indicated by a negative correlation between RGR during submergence and RGR post-submergence, (iii) an inverse relationship between RGR during recovery and S:R upon de-submergence, (iv) positive relationships between RGR at early recovery and RWC and gs, which were negatively related to S:R, suggesting this parameter as a good estimator of plant water balance post-submergence, (v) chlorophyll retention allowed fast recovery as revealed by the positive relationship between greenness of basal and apical leaves and RGR during the first recovery week, and (vi) full repair of the submergence-damaged photosynthetic apparatus occurred more slowly (second recovery week) than full recovery of plant water relations. The inclusion of these traits contributing to submergence recovery in L. japonicus should be considered to speed up the breeding process of the closely related forage Lotus spp. used in current agriculture.
Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation

The rare autosomal dominant Charcot-Marie-Tooth type 2B (CMT2B) is associated with mutations in the RAB7A gene, involved in the late endocytic pathway. CMT2B is characterized by predominant sensory loss, ulceromutilating features, with lesser-to-absent motor deficits. We characterized clinically and genetically a family harboring a novel pathogenic RAB7A variant and performed structural and functional analysis of the mutant protein. A 39-year-old woman presented with early-onset walking difficulties, progressive distal muscle wasting and weakness in lower limbs and only mild sensory signs. Electrophysiology demonstrated an axonal sensorimotor neuropathy. Nerve biopsy showed a chronic axonal neuropathy with moderate loss of all caliber myelinated fibers. Next-generation sequencing (NGS) technology revealed in the proband and in her similarly affected father the novel c.377A>G (p.K126R) heterozygous variant predicted to be deleterious. The mutation affects the biochemical properties of RAB7 GTPase, causes altered interaction with peripherin, and inhibition of neurite outgrowth, as for previously reported CMT2B mutants. However, it also shows differences, particularly in the epidermal growth factor receptor degradation process. Altogether, our findings indicate that this RAB7A variant is pathogenic and widens the phenotypic spectrum of CMT2B to include predominantly motor CMT2. Alteration of the receptor degradation process might explain the different clinical presentations in this family.
Association between traumatic brain injury and prison charges: a population-based cohort study

ABSTRACT Background Traumatic Brain Injury (TBI) is a serious hidden health issue disproportionately affecting people who experience incarceration. Objective We examined the association between TBI and serious disciplinary charges among men and women sentenced by the courts to terms of two or more years. Methods The study originated in Ontario, Canada and used linked administrative health and correctional data. The cohort included adults experiencing their first federal sentence between 1998 and 2011 (N = 12,038). We examined disciplinary charges incurred 2 years post-sentence commencement. TBI was defined using the International Classification of Diseases (ICD-9 and ICD-10) diagnostic codes. Robust Poisson regression was conducted to assess the association between TBI and disciplinary charges. Findings The prevalence of TBI for the full sample was 13.2%. One-third of adults with a recent TBI had a serious disciplinary charge. The unadjusted risk of incurring a serious charge for those with a history of TBI was 39% higher than those with no history of TBI (CI: 1.29–1.49). The adjusted risk was 1.14 (CI: 1.06–1.22). Conclusions TBI is a serious health concern that makes it difficult for incarcerants to adjust to prison. Additional support/resources are needed to support those with histories of TBI.
The Pathways and Processes Underlying Spinal Transmission of Low Back Pain: Observations From Dorsal Root Ganglion Stimulation Treatment

Dorsal root ganglion stimulation (DRG‐S) is a novel approach to treat chronic pain. Lead placement at L2 has been reported to be an effective treatment for axial low back pain (LBP) primarily of discogenic etiology. We have recently shown, in a diverse cohort including cases of multilevel instrumentation following extensive prior back surgeries, that DRG‐S lead placement at T12 is another promising target. Local effects at the T12 DRG, alone, are insufficient to explain these results.
Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble

In eukaryotic cilia and flagella, various types of axonemal dyneins orchestrate their distinct functions to generate oscillatory bending of axonemes. The force-generating mechanism of dyneins has recently been well elucidated, mainly in cytoplasmic dyneins, thanks to progress in single-molecule measurements, X-ray crystallography, and advanced electron microscopy. These techniques have shed light on several important questions concerning what conformational changes accompany ATP hydrolysis and whether multiple motor domains are coordinated in the movements of dynein. However, due to the lack of a proper expression system for axonemal dyneins, no atomic coordinates of the entire motor domain of axonemal dynein have been reported. Therefore, a substantial amount of knowledge on the molecular architecture of axonemal dynein has been derived from electron microscopic observations on dynein arms in axonemes or on isolated axonemal dynein molecules. This review describes our current knowledge and perspectives of the force-generating mechanism of axonemal dyneins in solo and in ensemble.
Correction Algorithm for the Navigation System of an Autonomous Unmanned Underwater Vehicle

More accurate navigation systems are always required for autonomous unmanned underwater vehicles (AUUV)s under various circumstances. In this paper, a measuring complex of a heavy unmanned underwater vehicle (UUV) was investigated. The measuring complex consists of an inertial navigation platform system, a Doppler lag (DL) and an estimation algorithm. During a relatively long-term voyage of an UUV without surfacing and correction from buoys and stationary stations, errors of the measuring complex will increase over time. The increase in errors is caused by an increase in the deviation angles of the gyro platform relative to the accompanying trihedron of the selected coordinate system. To reduce these angles, correction is used in the structure of the inertial navigation system (INS) using a linear regulator. To increase accuracy, it is proposed to take into account the nonlinear features of INS errors; an adaptive nonlinear Kalman filter and a nonlinear controller were used in the correction scheme. Considering that, a modified nonlinear Kalman filter and a regulator in the measuring complex are proposed to improve the accuracy of the measurement information, and modification of the nonlinear Kalman filter was performed through a genetic algorithm, in which the regulator was developed by the State Dependent Coefficient (SDC) method of the formulated model. Modeling combined with a semi-natural experiment with a real inertial navigation system for the UUV demonstrated the efficiency and effectiveness of the proposed algorithms.
CDKN3 regulates cisplatin resistance to colorectal cancer through TIPE1.

OBJECTIVE The aim of this study was to investigate the level of cyclin-dependent kinase inhibitor 3 (CDKN3) in colorectal cancer (CRC), to explore the underlying mechanism of CDKN3 in modulating cisplatin resistance and promoting the malignant progression of CRC. PATIENTS AND METHODS 43 pairs of CRC tissues and para-cancerous tissues were collected from CRC patients. CDKN3 expression was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between CDKN3 expression and the prognosis of CRC patients was analyzed. Meanwhile, qRT-PCR was performed to verify CDKN3 level in CRC cell lines. Next, CDKN3 knockdown model was constructed in CRC cisplatin-resistant cell lines. The influence of CDKN3 on the biological function of CRC cells was analyzed by Cell Counting Kit-8 (CCK-8) and plate cloning assays. Furthermore, the mechanism of its regulation of TIPE1 affecting cisplatin resistance to CRC was explored. RESULTS QRT-PCR results showed that CDKN3 level in CRC tissues was remarkably higher than that of the adjacent tissues (p<0.05). Compared with patients with low expression of CDKN3, the prognosis of patients with high expression of CDKN3 was significantly worse (p<0.05). Similarly, the proliferation and colony formation ability of cells in CDKN3 knockdown group remarkably decreased when compared with the sh-NC group (p<0.05). In addition, CDKN3 level was remarkably elevated in CRC patients with cisplatin resistance. In cisplatin-resistant cell lines (including HT28 and HCT-116), the knockdown of CDKN3 remarkably reduced cell viability (p<0.05). Furthermore, TIPE1 expression was remarkably downregulated in CRC tissues (p<0.05). A negative correlation was observed between the expressions of TIPE1 and CDKN3. Cell reverse experiment demonstrated that TIPE1 could reverse the promoting effect of CDKN3 on the malignant progression of CRC. All these findings suggested that there might exist a mutual regulation between CDKN3 and TIPE1. CONCLUSIONS CDKN3 was highly expressed in CRC, which might be closely correlated with poor prognosis of CRC patients. In addition, CDKN3 regulated cisplatin resistance to CRC by modulating TIPE1, thereby promoting the proliferation of CRC.
An Analysis of Public Opinions Regarding Take-Away Food Safety: A 2015–2018 Case Study on Sina Weibo

Take-away food (also referred to as “take-out” food in different regions of the world) is a very convenient and popular dining choice for millions of people. In this article, we collect online textual data regarding “take-away food safety” from Sina Weibo between 2015 and 2018 using the Octopus Collector. After the posts from Sina Weibo were preprocessed, users’ emotions and opinions were analyzed using natural language processing. To our knowledge, little work has studied public opinions regarding take-away food safety. This paper fills this gap by using latent Dirichlet allocation (LDA) and k-means to extract and cluster topics from the posts, allowing for the users’ emotions and related opinions to be mined and analyzed. The results of this research are as follows: (1) data analysis showed that the degree of topics have increased over the years, and there are a variety of topics about take-away food safety; (2) emotional analysis showed that 93.8% of the posts were positive; and (3) topic analysis showed that the topic of public discussion is diverse and rich. Our analysis of public opinion on take-away food safety generates insights for government and industry stakeholders to promote the healthy and vigorous development of the food industry.
Disruption of the cpsE and endA Genes Attenuates Streptococcus pneumoniae Virulence: Towards the Development of a Live Attenuated Vaccine Candidate

The majority of deaths due to Streptococcus pneumoniae infections are in developing countries. Although polysaccharide-based pneumococcal vaccines are available, newer types of vaccines are needed to increase vaccine affordability, particularly in developing countries, and to provide broader protection across all pneumococcal serotypes. To attenuate pneumococcal virulence with the aim of engineering candidate live attenuated vaccines (LAVs), we constructed knockouts in S. pneumoniae D39 of one of the capsular biosynthetic genes, cpsE that encodes glycosyltransferase, and the endonuclease gene, endA, that had been implicated in the uptake of DNA from the environment as well as bacterial escape from neutrophil-mediated killing. The cpsE gene knockout significantly lowered peak bacterial density, BALB/c mice nasopharyngeal (NP) colonisation but increased biofilm formation when compared to the wild-type D39 strain as well as the endA gene knockout mutant. All constructed mutant strains were able to induce significantly high serum and mucosal antibody response in BALB/c mice. However, the cpsE-endA double mutant strain, designated SPEC, was able to protect mice from high dose mucosal challenge of the D39 wild-type. Furthermore, SPEC showed 23-fold attenuation of virulence compared to the wild-type. Thus, the cpsE-endA double-mutant strain could be a promising candidate for further development of a LAV for S. pneumoniae.
Key Vitamin D Target Genes with Functions in the Immune System

The biologically active form of vitamin D3, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), modulates innate and adaptive immunity via genes regulated by the transcription factor vitamin D receptor (VDR). In order to identify the key vitamin D target genes involved in these processes, transcriptome-wide datasets were compared, which were obtained from a human monocytic cell line (THP-1) and peripheral blood mononuclear cells (PBMCs) treated in vitro by 1,25(OH)2D3, filtered using different approaches, as well as from PBMCs of individuals supplemented with a vitamin D3 bolus. The led to the genes ACVRL1, CAMP, CD14, CD93, CEBPB, FN1, MAPK13, NINJ1, LILRB4, LRRC25, SEMA6B, SRGN, THBD, THEMIS2 and TREM1. Public epigenome- and transcriptome-wide data from THP-1 cells were used to characterize these genes based on the level of their VDR-driven enhancers as well as the level of the dynamics of their mRNA production. Both types of datasets allowed the categorization of the vitamin D target genes into three groups according to their role in (i) acute response to infection, (ii) infection in general and (iii) autoimmunity. In conclusion, 15 genes were identified as major mediators of the action of vitamin D in innate and adaptive immunity and their individual functions are explained based on different gene regulatory scenarios.
Building a Machine-Learning Framework to Remotely Assess Parkinson's Disease Using Smartphones

Objective: Parkinson's disease (PD) is a neurodegenerative disorder that affects multiple neurological systems. Traditional PD assessment is conducted by a physician during infrequent clinic visits. Using smartphones, remote patient monitoring has the potential to obtain objective behavioral data semi-continuously, track disease fluctuations, and avoid rater dependency. Methods: Smartphones collect sensor data during various active tests and passive monitoring, including balance (postural instability), dexterity (skill in performing tasks using hands), gait (the pattern of walking), tremor (involuntary muscle contraction and relaxation), and voice. Some of the features extracted from smartphone data are potentially associated with specific PD symptoms identified by physicians. To leverage large-scale cross-modality smartphone features, we propose a machine-learning framework for performing automated disease assessment. The framework consists of a two-step feature selection procedure and a generic model based on the elastic-net regularization. Results: Using this framework, we map the PD-specific architecture of behaviors using data obtained from both PD participants and healthy controls (HCs). Utilizing these atlases of features, the framework shows promises to (a) discriminate PD participants from HCs, and (b) estimate the disease severity of individuals with PD. Significance: Data analysis results from 437 behavioral features obtained from 72 subjects (37 PD and 35 HC) sampled from 17 separate days during a period of up to six months suggest that this framework is potentially useful for the analysis of remotely collected smartphone sensor data in individuals with PD.
Sexual Recognition in Adult Erythemis Simplicicollis (Odonata: Anisoptera)

This research was conducted primarily to determine if male dragonflies of the dimorphic species Erythemis simplicicollis reacted differently towards the different sexes, and, if so, to find out what sexual differences served as stimuli for the various behavioral patterns. Altogether thirty-five experiments were conducted in the marsh of Ebaugh Pond, Denison University, utilizing St. Quentin's fishing-line technique (1934) to simulate natural conditions. The experimentation involved altering the physical appearance of both live and dead dragonflies of both sexes and observing the reaction of free males towards these specimens. It was concluded that the visual recognition in this dimorphic species involved stimulation by perception of body coloration, and did not include any recognition of variation in flight pattern or any other subtle difference in behavior. '
Atypical Multibacterial Granulomatous Myositis in a Horse: First Report in Italy

Infectious causes of myositis are reported relatively uncommonly in horses. Among them, bacterial causes include Streptococcus equi subsp. zooepidemicus, Actinobacillus equuli, Fusobacterium spp. Staphylococcus spp, and Corynebacterium pseudotuberculosis. Infection can be spread to muscles via haematogenous or extension from skin lesions. Parasitic myositis has also been documented. In this report, a 12 year-old Italian Quarter Horse mare presented with diffuse subcutaneous nodules and masses ranging from 2 × 3 to 5 × 20 cm in size, and adherent to subcutis and muscles that were first macroscopically and cytologically diagnosed as pyogranulomas. Subsequently, histological, molecular, bacteriological, and biochemical investigations were performed. All the data obtained allowed to diagnose a severe and diffuse multibacterial granulomatous myositis caused by Corynebacterium pseudotuberculosis and Corynebacterium amycolatum. Following the therapy and an initial disappearance of most of the lesions together with a general improvement of the mare, the clinical condition deteriorated, and new nodules appeared. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and PCR techniques revealed the presence of bacteria as Glutamicibacter creatinolyticus and Dietzia spp. To the authors’ knowledge, this case report represents the first description of multibacterial granulomatous myositis due to Corynebacterium pseudotuberculosis, Corynebacterium amycolatum, Glutamicibacter creatinolyticus, and Dietzia spp. in a horse reared in Italy.
Mental and substance use disorders and food insecurity among homeless adults participating in the At Home/Chez Soi study

Background Few studies have examined how food insecurity changes over time when living with severe mental disorders or substance use disorders. This study identifies food insecurity trajectories of homeless adults participating in a trial of a housing intervention and examines whether receiving the intervention and having specific mental and substance disorders predict food insecurity trajectories. Materials and methods We studied 520 participants in the Toronto site of the At Home/Chez-Soi project. Food insecurity data were collected at seven times during a follow-up period of up to 5.5 years. Mental and substance use disorders were assessed at baseline. Food insecurity trajectories were identified using group based-trajectory modeling. Multinomial logistic regression was used to examine the effects of the intervention and mental and substance use disorders on food insecurity trajectories. Results Four food insecurity trajectories were identified: persistently high food insecurity, increasing food insecurity, decreasing food insecurity, and consistently low food insecurity. Receiving the intervention was not a predictor of membership in any specific food insecurity trajectory group. Individuals with major depressive episode, mood disorder with psychotic features, substance disorder, and co-occurring disorder (defined as having at least one alcohol or other substance use disorder and at least one non-substance related mental disorder] were more likely to remain in the persistently high food insecurity group than the consistently low food insecurity group. Conclusion A persistently high level of food insecurity is common among individuals with mental illness who have experienced homelessness, and the presence of certain mental health disorders increases this risk. Mental health services combined with access to resources for basic needs, and re-adaptation training are required to enhance the health and well-being of this population.