Miracle-dz/UniFAR-FTUs huggingface dataset:
Chemokine Receptor CXCR-2 Initiates Atrial Fibrillation by Triggering Monocyte Mobilization in Mice

Supplemental Digital Content is available in the text. Atrial fibrillation (AF) is frequently associated with increased inflammatory response characterized by infiltration of monocytes/macrophages. The chemokine receptor CXCR-2 is a critical regulator of monocyte mobilization in hypertension and cardiac remodeling, but it is not known whether CXCR-2 is involved in the development of hypertensive AF. AF was induced by infusion of Ang II (angiotensin II; 2000 ng/kg per minute) for 3 weeks in male C57BL/6 wild-type mice, CXCR-2 knockout mice, bone marrow-reconstituted chimeric mice, and mice treated with the CXCR-2 inhibitor SB225002. Microarray analysis revealed that 4 chemokine ligands of CXCR-2 were significantly upregulated in the atria during 3 weeks of Ang II infusion. CXCR-2 expression and the number of CXCR2+ immune cells markedly increased in Ang II–infused atria in a time-dependent manner. Moreover, Ang II–infused wild-type mice had increased blood pressure, AF inducibility, atrial diameter, fibrosis, infiltration of macrophages, and superoxide production compared with saline-treated wild-type mice, whereas these effects were significantly attenuated in CXCR-2 knockout mice and wild-type mice transplanted with CXCR-2-deficient bone marrow cells or treated with SB225002. Moreover, circulating blood CXCL-1 levels and CXCR2+ monocyte counts were higher and associated with AF in human patients (n=31) compared with sinus rhythm controls (n=31). In summary, this study identified a novel role for CXCR-2 in driving monocyte infiltration of the atria, which accelerates atrial remodeling and AF after hypertension. Blocking CXCR-2 activation may serve as a new therapeutic strategy for AF.
The role of preoperative blood eosinophil counts in distinguishing chronic rhinosinusitis with nasal polyps phenotypes

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common heterogenous disease in the patients with chronic airway diseases. This study investigated the role of blood eosinophil count (BEC) in the classification of CRSwNP and its recurrence in eosinophilic CRSwNP.
Growth of twisted bilayer graphene through two-stage chemical vapor deposition

We investigate growth of twisted bilayer graphene through two-stage chemical vapor deposition (CVD). Exploiting the synergetic nucleation and growth dynamics involving carbon sources from the residual carbon impurities in Cu bulk and gaseous CHx, sub-millimeter-sized single crystalline graphene grains with multiple merged adlayer grains formed underneath are grown on Cu substrate. The distribution of the twist angles is investigated through a computer algorithm utilizing spectral features from micro-Raman mapping. Besides the more thermodynamically stable AB-stacking (AB-BLG) or large angle (>15°) decoupled bilayer graphene (DC-BLG) configurations, there are some bilayer regions that contain specific twist angles (3–8°, 8–13°, and 11–15°) (termed as TBLG). The statistics show no TBLG formation for BLG with single nucleation center. The formation probability of TBLG is strongly dependent on the relative orientation of merging adlayer grains. Significant defects are found at the grain boundaries formed in AB-DC merging event without creating TBLG domain. The areal fraction of TBLG increases as H2/CH4 ratio increases. The growth mechanism of TBLG is discussed in light of the interactions between the second layer grains with consideration of strain generation during merging of adlayers.
Demography and Phylogenetic Utility of Numt Pseudogenes in the Southern Red-Backed Vole (Myodes gapperi)

Abstract Nuclear copies of mitochondrial DNA fragments (numts) have been reported in a number of mammalian taxa. The inclusion of these numt pseudogenes within mitochondrial data sets can unknowingly compromise the integrity of phylogenetic, systematic, and biogeographic studies. Here, we evaluate 6 unique cytochrome-b numt sequences isolated in the southern red-backed vole (Myodes [formerly Clethrionomys] gapperi). Each sequence contains features characteristic of numt pseudogenes including premature stop codons, insertions–deletions (indels), frame-shift substitutions, and transposable element insertions. Mitochondrial and nuclear sequences are evolving at different rates. Molecular dating indicates that this group of mitochondrial transfers originated ∼4.6 million years ago, which predates the presumptive origin of the genus. Phylogenetic analyses of mitochondrial and nuclear sequences suggest that the numts are the result of at least 1 nuclear insertion followed by subsequent duplication events, but pairwise sequence comparisons do not support this assertion. This conflict is likely the result of incomplete numt sampling from the Myodes nuclear genome. Genome sequencing efforts will ultimately provide the data needed to fully characterize numt demography in different lineages, and we predict that numts will have phylogenetic utility as informative synapomorphies in closely related species.
Induction de la précipitation phosphocalcique dans le perméat de lactosérum de fromagerie

Résumé La précipitation phosphocalcique a été induite de 2 façons dans le perméat de lactosérum de fromagerie : soit par alcalinisation simple, soit par effet combiné alcalinisationensemencement du milieu par des cristaux de phosphate dicalcique. L'alcalinisation simple s'est avérée inefficace, alors que l'ensemencement a provoqué une cristallisation intense, accompagnée d'une baisse de calcium et de phosphate solubles du perméat. Sur une zone de pH comprise entre 6,6 et 8,0, à 25 et 50 "C, il est apparu que les conditions pH 8,0 et 50 oC provoquaient la précipitation la plus complète, soit 61 % du calcium et 32% du phosphate solubles. L'observation microscopique et la stoechiométrie du précipité obtenu dans ces conditions suggèrent qu'un précipité amorphe et plus alcalin que la phase de nucléation est formé.
Association between Egg Consumption and Cholesterol Concentration: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

The association of egg consumption and serum cholesterol concentrations in healthy people has been discussed for a long time. In this study, we aimed to explore association of egg consumption with on low-density lipoprotein cholesterol (LDL-c) and high-density lipoprotein cholesterol (HDL-c) concentrations and the LDL-c/HDL-c ratio through meta-analysis. This systematic review only included randomized controlled trials (RCTs) investigating egg consumption in healthy populations without combination therapy. We extracted mean and standard deviation for LDL-c/HDL-c ratio, LDL-c/HDL-c. The extracted data were pooled in a random-effects model and were presented as mean difference (MD) with 95% confidence interval (CI). Moreover, subgroup analyses were conducted for understanding effects of more egg consumption (MEC) on different intervention periods, egg-consumption levels, classification of responders. Overall, 17 RCTs met the eligibility criteria and pooled results showed MEC group had a higher LDL-c/HDL-c ratio than the control group (MD = 0.14, p = 0.001, I2 = 25%). The MEC group also had higher LDL-c than the control group (MD = 8.14, p < 0.0001, I2 = 18%). Moreover, for the subset of intervention over two months, the MEC group seemed to have a larger effect size than the subset of intervention within two months. This synthesis, the largest meta-analysis on this topic, shows the impact of egg consumption on lipid profiles among healthy subjects. Notably, longer time with MEC may lead to higher LDL-c/HDL-c ratio and LDL-c. However, RCTs with long tern follow-up are needed to guarantee the association between egg consumption and human health.
Detection of punctuated equilibrium from molecular phylogenies

Abstract The theory of ‘punctuated equilibrium’ hypothesises that most morphological change in species takes place in rapid bursts triggered by speciation. Eldregde and Gould postulated the theory in 1972, as an alternative to the idea that morphological change slowly accumulates in the course of time, a then common belief they dubbed ‘phyletic gradualism’. Ever since its introduction the theory of punctuated equilibrium has been the subject of speculation rather than empirical validation. Here I present a method to detect punctuated evolution without reference to fossil data, based on the phenotypes of extant species and on their relatedness as revealed by molecular phylogeny. The method involves a general mathematical model describing morphological differentiation of two species over time. The two parameters in the model, the rates of punctual (cladogenetic) and gradual (anagenetic) change, are estimated from plots of morphological diversification against time since divergence of extant species.
A Simplified Method of Synthesis to Obtain Zwitterionic Cellulose under Mild Conditions with Active Ionic Moieties

A simplified procedure to synthesize zwitterionic cellulose by means of N-protected aspartic anhydride under mild conditions was developed. The preparation of modified cellulose samples was carried out under heterogeneous, aqueous conditions by reacting NH4OH-activated cellulose with aspartic anhydrides N-protected with trifluoroacetyl (TFAc) and carbobenzyloxy (Cbz). Modified cellulose samples Cel-Asp-N-TFAc and Cel-Asp-N-Cbz were characterized by Fourier Transform Infrared (FTIR) and 13C solid state Nuclear Magnetic Resonance (NMR) spectroscopy. The functionalization degree of each cellulose sample was determined by the 13C NMR signal integration values corresponding to the cellulose C1 vs. the Cα of the aspartate residue and corroborated by elemental analysis. In agreement, both analytical methods averaged a grafting degree of 20% for Cel-Asp-N-TFAc and 16% for Cel-Asp-N-Cbz. Conveniently, Cel-Asp-N-TFAc was concomitantly partially N-deprotected (65%) as determined by the ninhydrin method. The zwitterion character of this sample was confirmed by a potentiometric titration curve and the availability of these amino acid residues on the cellulose was inspected by adsorption kinetics method with a 100 mg L−1 cotton blue dye solution. In addition, the synthesis reported in the present work involves environmentally related advantages over previous methodologies developed in our group concerning to zwitterionic cellulose preparation.
A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly

Nutrient-transporting enterocytes interact with their luminal environment using a densely packed collection of apical microvilli known as the brush border. Assembly of the brush border is controlled by the intermicrovillar adhesion complex (IMAC), a protocadherin-based complex found at the tips of brush border microvilli that mediates adhesion between neighboring protrusions. ANKS4B is known to be an essential scaffold within the IMAC, although its functional properties have not been thoroughly characterized. We report here that ANKS4B is directed to the brush border using a noncanonical apical targeting sequence that maps to a previously unannotated region of the scaffold. When expressed on its own, this sequence targeted to microvilli in the absence of any direct interaction with the other IMAC components. Sequence analysis revealed a coiled-coil motif and a putative membrane-binding basic-hydrophobic repeat sequence within this targeting region, both of which were required for the scaffold to target and mediate brush border assembly. Size-exclusion chromatography of the isolated targeting sequence coupled with in vitro brush border binding assays suggests that it functions as an oligomer. We further show that the corresponding sequence found in the closest homolog of ANKS4B, the scaffold USH1G that operates in sensory epithelia as part of the Usher complex, lacks the inherent ability to target to microvilli. This study further defines the underlying mechanism of how ANKS4B targets to the apical domain of enterocytes to drive brush border assembly and identifies a point of functional divergence between the ankyrin repeat–based scaffolds found in the IMAC and Usher complex.
Selecting optimal spectral bands for improved detection of autofluorescent biomarkers in multiphoton microscopy

Abstract. Significance: In multiphoton microscopy, two-photon excited fluorescence (TPEF) spectra carry valuable information on morphological and functional biological features. For measuring these biomarkers, separation of different parts of the fluorescence spectrum into channels is typically achieved by the use of optical band pass filters. However, spectra from different biomarkers can be unknown or overlapping, creating a crosstalk in between the channels. Previously, establishing these channels relied on prior knowledge or heuristic testing. Aim: The presented method aims to provide spectral bands with optimal separation between groups of specimens expressing different biomarkers. Approach: We have developed a system capable of resolving TPEF with high spectral resolution for the characterization of biomarkers. In addition, an algorithm is created to simulate and optimize optical band pass filters for fluorescence detection channels. To demonstrate the potential improvements in cell and tissue classification using these optimized channels, we recorded spectrally resolved images of cancerous (HT29) and normal epithelial colon cells (FHC), cultivated in 2D layers and in 3D to form spheroids. To provide an example of an application, we relate the results with the widely used redox ratio. Results: We show that in the case of two detection channels, our system and algorithm enable the selection of optimized band pass filters without the need of knowing involved fluorophores. An improvement of 31,5% in separating different 2D cell cultures is achieved, compared to using established spectral bands that assume NAD(P)H and FAD as main contributors of autofluorescence. The compromise is a reduced SNR in the images. Conclusions: We show that the presented method has the ability to improve imaging contrast and can be used to tailor a given label-free optical imaging system using optical band pass filters targeting a specific biomarker or application.
Nomenclature and typification of subdivisional names in the genus Peperomia (Piperaceae)

The genus Peperomia includes 1,500-1,700 accepted species, making it one of the giant genera ofangiosperms. The infrageneric classification usually adopted is that of Dahlstedt (1900) which divides Peperomia into 9 subgenera, 7 sections and 4 subsections. In 1930, two of these sections were treated comparably with Dahlstedt's subgenera, making a total of 11 primary subdivisions of Peperomia. Later, different authors added a few more subgenera to include some island species. The names of only a few subgenera, sections or subsections of Peperomia have been typified. In this paper, we present a survey of all subgenera, sections and subsections described in the genus Peperomia and we designate nomenclatural types for names that have not yet been typified.
Genetic Reconstruction and Forensic Analysis of Chinese Shandong and Yunnan Han Populations by Co-Analyzing Y Chromosomal STRs and SNPs

Y chromosomal short tandem repeats (Y-STRs) have been widely harnessed for forensic applications, such as pedigree source searching from public security databases and male identification from male–female mixed samples. For various populations, databases composed of Y-STR haplotypes have been built to provide investigating leads for solving difficult or cold cases. Recently, the supplementary application of Y chromosomal haplogroup-determining single-nucleotide polymorphisms (SNPs) for forensic purposes was under heated debate. This study provides Y-STR haplotypes for 27 markers typed by the Yfiler™ Plus kit and Y-SNP haplogroups defined by 24 loci within the Y-SNP Pedigree Tagging System for Shandong Han (n = 305) and Yunnan Han (n = 565) populations. The genetic backgrounds of these two populations were explicitly characterized by the analysis of molecular variance (AMOVA) and multi-dimensional scaling (MDS) plots based on 27 Y-STRs. Then, population comparisons were conducted by observing Y-SNP allelic frequencies and Y-SNP haplogroups distribution, estimating forensic parameters, and depicting distribution spectrums of Y-STR alleles in sub-haplogroups. The Y-STR variants, including null alleles, intermedia alleles, and copy number variations (CNVs), were co-listed, and a strong correlation between Y-STR allele variants (“DYS518~.2” alleles) and the Y-SNP haplogroup QR-M45 was observed. A network was reconstructed to illustrate the evolutionary pathway and to figure out the ancestral mutation event. Also, a phylogenetic tree on the individual level was constructed to observe the relevance of the Y-STR haplotypes to the Y-SNP haplogroups. This study provides the evidence that basic genetic backgrounds, which were revealed by both Y-STR and Y-SNP loci, would be useful for uncovering detailed population differences and, more importantly, demonstrates the contributing role of Y-SNPs in population differentiation and male pedigree discrimination.
Real-space imaging of a single-molecule monoradical reaction.

Organic radicals consisting of light elements exhibit a low spin-orbit coupling and weak hyperfine interactions with a long spin coherence length, which are crucial for future applications in molecular spintronics. However, the synthesis and characterization of these organic radicals have been a formidable challenge due to their chemical instability arising from unpaired electrons. Here, we report a direct imaging of the surface chemical transformation of an organic monoradical synthesized via the monodehydrogenation of a chemically designed precursor. Bond-resolved scanning tunneling microscopy unambiguously resolves various products formed through a complex structural dissociation and rearrangement of organic monoradicals. Density functional theory calculations reveal detailed reaction pathways from the monoradical to different cyclized products. Our study provides unprecedented insights into complex surface reaction mechanisms of organic radical reactions at the single molecule level, which may guide the design of stable organic radicals for future quantum technology applications.
matFR: a MATLAB toolbox for feature ranking

SUMMARY Nowadays, it is feasible to collect massive features for quantitative representation and precision medicine, and thus, automatic ranking to figure out the most informative and discriminative ones becomes increasingly important. To address this issue, 42 feature ranking (FR) methods are integrated to form a MATLAB toolbox (matFR). The methods apply mutual information, statistical analysis, structure clustering and other principles to estimate the relative importance of features in specific measure spaces. Specifically, these methods are summarized and an example shows how to apply a FR method to sort mammographic breast lesion features. The toolbox is easy to use and flexible to integrate additional methods. Importantly, it provides a tool to compare, investigate and interpret the features selected for various applications. AVAILABILITY AND IMPLEMENTATION The toolbox is freely available at http://github.com/NicoYuCN/matFR. A tutorial and an example with a data set are provided.
A Single-Dataset-Based Pre-Processing Joint Domain Localized Algorithm for Clutter-Suppression in Shipborne High-Frequency Surface-Wave Radar

Due to the motion of the platform, the spectrum of first-order sea clutter will widen and mask low-velocity targets such as ships in shipborne high-frequency surface-wave radar (HFSWR). Limited by the quantity of qualified training samples, the performance of the generally used clutter-suppression method, space–time adaptive processing (STAP) degrades in shipborne HFSWR. To deal with this problem, an innovative training sample acquisition method is proposed, in the area of joint domain localized (JDL) reduced-rank STAP. In this clutter-suppression method, based on a single range of cell data, the unscented transformation is introduced as a preprocessing step to obtain adequate homogeneous secondary data and roughly estimated clutter covariance matrix (CCM). The accurate CCM is calculated by integrating the approximate CCM of different range of cells. Compared with existing clutter-suppression algorithms for shipborne HFSWR, the proposed approach has a better signal-to-clutter-plus-noise ratio (SCNR) improvement tested by real data.
Molecular and morphological evidence that Phymactis papillosa from Argentina is, in fact, a new species of the genus Bunodosoma (Cnidaria: Actiniidae)

Phymactis papillosa is a rocky shore sea anemone that is commonly found in the Pacific Ocean, from the Gulf of California to Tierra del Fuego, and in the Mar del Plata region, Argentina. The genus Phymactis is closely related to Bunodosoma and, due to character plasticity, a number of misidentifications have occurred. Therefore, the presence of P. papillosa in Argentina has been doubted but the matter had not been investigated in detail. Here we analyse P. papillosa specimens from Argentina and compare them, using molecular and morphological markers, to specimens from the species' type locality. In a phylogenetic analysis using 19 allozyme markers and ribosomal internal transcribed spacers sequences of different sea anemone genera, including all West Atlantic Bunodosoma species, we have found that the specimens from Argentina were genetically divergent from P. papillosa from Chile and closely related to West Atlantic Bunodosoma species. The genetic and morphological analyses indicate that those specimens belong to a new species of the genus Bunodosoma, described here as B. zamponii sp. nov.
Recombinant influenza viruses as a vaccine vector for HIV

Live recombinant influenza viruses were successfully used as HIV vaccine vectors in a mouse model. Following intranasal prime-boost vaccination, HIV-specific CD8+ T cell responses were detected in the spleen, broncho-alveolar lavage, mediastinal and inguinal lymph nodes. HIV+α4β7+ CD8+ T cells contributed to protection in pseudo-challenge experiments using recombinant vaccinia virus expressing HIV antigens. This research highlights the importance of mucosal CD8+ T cells in viral immunity and emphasizes the need for additional studies to provide key insights to underpin future vaccine development.
ANTIMICROBIAL AND ANTIOXIDANT PROTEINS FROM THE CRAB LIAGORE RUBROMACULATA (DE HAAN, 1835)

The emergence of new infectious diseases and resistance to the antibiotics by the existing ones led to the new sources for drug discovery. In the present study antibacterial and antioxidant activity of the hemolymph of brachyuran crabs Liagore rubromaculata was investigated. In the antibacterial activity against 6 pathogenic strains maximum zone of inhibition was recorded against highest zone of inhibition was observed in the hemolymph of female crab L. rubromaculata against P. vulgaris(12 ± 1 mm) and the minimum activity was observed against E.coli(6 ± 1 mm). In the hemolymph of male crab L.rubromaculata the highest zone of inhibition was observed Against E. faecalis(9 ± 1 mm) and the minimum activity observed against K. oxytoca (7mm). In TLC appearance of pink spots indicates the presence of protein in the tested sample. In SDS PAGE, in male four bands were detected in the gel that represents the presence of proteins in the range nearly 26.5-73 kDa. In female five bands were detected in the gel that represents the presence of proteins in the range nearly 25.2-75 k Da. Hence the present study indicated that the hemolymph of L. rubromaculata crabs may potential antibiotics.