Miracle-dz/UniFAR-FTUs huggingface dataset:
Cobertura vegetal do estado de São Paulo: I - Levantamento por fotointerpretação das áreas cobertas com cerrado, cerradão e campo, em 1962

Foi realizado, com base em fotografias aereas, o levantamento da cobertura vegetal de cerrado no Estado de Sao Paulo, em 1962. Separaram-se as areas com o cerrado propriamente dito (tipo da savana brasileira), cerradao (tipo de cerrado mais alto e mais denso) e de campo (tipo de vegetacao herbacea). Na caracterizacao dessas coberturas, levaram-se em conta apenas as areas ainda sem utilizacao agricola e com a vegetacao pouco alterada pelo homem. A fotointerpretacao foi feita com base na caracterizacao das texturas por amostragem de campo. O trabalho mostrou a situacao seguinte: cerradao. 724.900, cerrado 2.668. 000, e campo, 458.600 ha, representando, respectivamente, 2,9, 10,8 e 1,7% da area do Estado.
Determinants of the difficulty of leadless pacemaker implantation

The Micra Transcatheter Pacing System is implanted directly in the right ventricle (RV) through the femoral vein using a steerable transcatheter delivery system. The present study was done to identify determinants of difficult leadless pacemaker implant procedures including operator, patient, and RV anatomical characteristics.
17 β‐estradiol transiently disrupts adherens junctions in endothelial cells

Interendothelial junctions are important regulators of endothelial cell functions such as migration and proliferation, major features in angiogenesis, and endothelial cell monolayer wound healing. 17β‐estradiol regulates these functions in vivo and in vitro and also increases endothelial monolayer permeability as it results from impaired monolayer integrity and intercellular adhesion. We hypothesized that 17β‐estradiol affects these cell adhesion‐dependent functions in endothelial cells by targeting the adherens junction complex. Here, we show that 17β‐estradiol increases uterine microvascular endothelial cell monolayer permeability and transiently redistributes interendothelial junction‐forming proteins in endothelial cells. Concomitantly, adherens junction proteins are disconnected from the cytoskeleton and α‐catenin, which links VE‐cadherin to the cytoskeleton, is redistributed from the membrane and the adherens junction complex. Furthermore, 17β‐estradiol increased tyrosine phosphorylation of the adherens junction complex. These effects were inhibited by the estrogen receptor antagonist ICI 182,780 but could be provoked using non‐cell membrane‐permeable 17β‐estradiol‐BSA in all cells tested, including EA.hy 926 cells, which have been shown unable to stimulate 17β‐estradiol‐dependent gene transcription. Additionally, 17β‐estradiol treatment enhanced the angiogenic effect of vascular endothelial growth factor in an in vitro angiogenesis model, as a potential implication of the adherens junction disruption. Cotreatment with the Src‐family kinase inhibitor PP2 prevented the redistribution and phosphorylation of the adherens junction proteins. Taken together, our data show that adherens junctions in endothelial cells are a downstream target of membrane‐associated 17β‐estradiol signaling, possibly through Src‐family kinases.
Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress

Objectives To investigate the antidepressant effects of electroacupuncture (EA) on chronic unpredictable mild stress (CUMS) in rats, as well as the effects of EA on hippocampal neurons, synaptic morphology, and 5-hydroxytryptamine (HT) receptor expression. Methods Forty adult male Wistar rats were randomly divided into normal control, CUMS, EA, and paroxetine groups. CUMS modeling was performed for 21 days, followed by 14 days of intervention: rats in the EA group underwent stimulation of GV20 and GV29 acupuncture points for 30 minutes daily; rats in the paroxetine group were administered paroxetine daily. Behavioral tests, transmission electron microscopy, western blotting, and real-time quantitative polymerase chain reaction were used to evaluate the effects of the intervention. Results EA treatment reversed the behavioral changes observed in rats due to CUMS modeling; it also improved the pathological changes in organelles and synaptic structures of hippocampal neurons, and upregulated the protein and mRNA expression levels of 5-HT1A receptor. There were no significant differences in 5-HT1B receptor protein and mRNA expression levels among the groups. Conclusions EA treatment can alleviate depression-like symptoms in CUMS rats. The underlying mechanism may include promoting the expression of 5-HT1A receptor mRNA and protein, thereby improving synaptic plasticity in the hippocampus.
Comparison of the clinical characteristics and outcomes of bloodstream infections caused by Raoultella species and Klebsiella pneumoniae

Abstract Background: There have been few clinical studies of Raoultella infections but Raoultella is potentially virulent and multidrug resistant. The aims of the study were to compare the clinical characteristics and outcomes of patients with Raoultella and Klebsiella pneumoniae bloodstream infections (BSIs). Methods: We retrospectively reviewed the records of patients with Raoultella and K. pneumoniae BSIs admitted to a tertiary hospital between 2008 and 2017. Results: A total of 37 cases of Raoultella BSIs were identified and matched to 160 cases of K. pneumoniae BSIs by age and sex using propensity score matching. Patients with Raoultella BSIs were more likely to have underlying biliary tract disease (54.1% versus 24.4%; p < .001) and have a community-acquired infection (62.2% versus 43.1%; p = .04) than those with K. pneumoniae BSIs. Intra-abdominal infection was the most common primary focus of infection. Biliary tract infection (64.9% versus 38.8%; p = .004) and pancreatitis (13.5% versus 3.8%; p = .03) were more common in patients with Raoultella BSIs. Raoultella isolates exhibited significantly higher susceptibility to aztreonam, cefepime, and cefotaxime. The 14-day and 30-day mortality rates were lower among the patients with Raoultella BSIs but did not differ significantly between groups (11% versus 22%; p = .16 and 11% versus 26%; p = .08 for Raoultella and K. pneumoniae BSIs, respectively). Conclusion: Raoultella spp. BSI more likely to occur in patients with underlying biliary tract disease and in community settings compared with K. pneumoniae BSIs. Biliary tract infection was the most common primary focus of Raoultella BSIs.
Differences in reproducibility of gait variability and fractal dynamics according to walking duration

BACKGROUND: Gait variability and fractal dynamics may be affected by the walking duration. OBJECTIVE: The purpose of this study is to examine the reproducibility of stride time while walking on a self-paced treadmill. METHODS: Fifteen young and healthy subjects walked on the treadmill for 10 minutes. Three to eight minutes duration of the data were used to compare the trial-to-trial and day-to-day reproducibility of the average, variability, and fractal dynamics of stride time. RESULTS: The results show that all variables had high trial-to-trial reproducibility. In the day-to-day results, the average walking speed and mean stride time showed reproducibility without regard for duration, but the variability and gait fractal dynamics showed differences in reproducibility according to duration. The variability and fractal dynamics showed better reproducibility in less than 5 minutes and over time, respectively. However, both variables generally showed improved reproducibility when average data from two to three rounds were used. CONCLUSION: Based on the results of this study, it is proposed that variability should be examined using data of 5 min or less, and fractal dynamics should be examined using 5 min or more of repeated data when performing walking tests from a gait dynamics perspective.
What Differentiates Prisoners Who Attempt Suicide from Those Who Experience Suicidal Ideation? A Nationally Representative Study.

OBJECTIVE Many people who think about suicide do not engage in suicidal behavior. Identifying risk factors implicated in the process of behavioral enaction is crucial for suicide prevention, particularly in high-risk groups such as prisoners. METHOD Cross-sectional data were drawn from a nationally representative sample of 17,891 prisoners (79% men) in the United States. We compared prisoners who attempted suicide (attempters; n = 2,496) with those who thought about suicide but never made an attempt (ideators; n = 1,716) on a range of established risk factors. RESULTS More than half (59%) of participants who experienced suicidal ideation had also attempted suicide. Violent offending, trauma, brain injury, alcohol abuse, and certain mental disorders distinguished attempters from ideators. CONCLUSION Our results fit within recent ideation-to-action theories that emphasize the role of a capability for suicide in the transition from thoughts to acts of suicide.
Micropropagation of Abelmoschus esculentus L. (Moench.) for disease free plantlets through meristem culture

Protocol was established for mass in vitro propagation of okra using meristem culture. Meristems (0.3–0.5 mm in size) were isolated from shoot tips of three-week old in vitro grown seedlings. Isolated meristems were established rapidly in MS liquid medium containing 1.0 mg/l of BAP. For shoot development from primarily established meristem, semisolid MS medium having the same concentration of BAP was found to be the most effective. Rapid shoot multiplication of mericlone was achieved from node cutting cultured in 1.0 mg/l plus 0.5 mg/l GA3, and a maximum of nine shoots were found from each node. Effective root development from the developed plantlets was successful in 1.0 mg/l IBA. More than 75% of the micropropagated mericlones plantlets were successfully acclimatised in soil up to maturity and found to be healthy.
Effect of the duration of feeding of a protein‐free diet on endogenous ileal nitrogen and amino acid loss in the growing pig

The aim of the study was to determine whether endogenous nitrogen and amino acid excretions at the terminal ileum change over time in the growing pig fed a protein-free diet for 8 days. Seven entire male pigs with an overall mean live weight of 81.6 kg (SEM 3.3 kg) and surgically implanted post-valve T caecum cannulas were fed a semi-synthetic casein-based diet for 8 days. Food was withheld from the pigs for 24 h, after which they were fed a protein-free diet for a further 8 days at a rate of 10% of metabolic body weight per day. Chromic oxide was included in the protein-free diet as an indigestible marker. Ileal digesta were collected continuously from 13:00 to 18:00 h on each day of the experimental period. Endogenous ileal nitrogen flows were determined for each pig each day the protein-free diet was given, and endogenous ileal amino acid flows for the first and eighth days. There were no significant (P > 0.05) effects of the duration of feeding of the protein-free diet on endogenous ileal total nitrogen or amino acid flows, except for the amino acids glycine and cysteine, the flows of which significantly decreased over the 8 day period (P < 0.01 and P < 0.05 for glycine and cysteine respectively), from (mean ± SEM) 1639 ± 217 to 892 ± 212 µg g−1 dry matter intake (DMI) for glycine and from 173 ± 13 to 127 ± 19 µg g−1 DMI for cysteine. The relative contributions (moles of each amino acid as a proportion of total moles of amino acids) of threonine, glycine and cysteine decreased significantly (P < 0.05) and that of proline increased significantly (P < 0.05) during the 8 days that the protein-free diet was fed to the pigs. © 2000 Society of Chemical Industry
Dietary exposure of Tunisian adult population aged from 19 to 65 years old to pesticides residues

ABSTRACT Dietary exposure of the Tunisian adult population to pesticide residue was assessed using the Total Diet Study method. In the present study 170 pesticides were searched for in 42 aggregated foods characterised by 64 food samples representing the Tunisian diet. All the food samples were collected, prepared, and analysed for remains of pesticides including organochlorine, organophosphorous, carbamates and pyrethroids. The GC-MS analysis and the LC-MS/MS analysis and some other specific analytical methods were employed for the quantification of the pesticide residues in the food samples. Results revealed that21pesticides reached amounts greater than the LOQ(12.3%) and 149 pesticides reached amounts below the LOD (88%). For the 21 pesticides quantified, the ADI was not exceeded. For the 149 non-quantified pesticides, an interval defined by the lower and upper bounds was defined to assess the dietary exposure of the Tunisian adult population to those pesticides. We conclude that 8 pesticides theoretically exceed the ADI with the 95th percentile of exposure, those are: Diféthialone, Brodifacoum, Bromadiolone, Glufosinate, Heptachlor, Dieldrin Aldrin Oxydemeton-methyl. This study concludes that there is a low dietary exposure to pesticide residue of the Tunisian adult population. In fact, all the cases where the ADI was exceeded were theoretical due to the lower ADI value used.
The Perspectives of Australian Naturopaths about Providing Health Services for People with Sleep Disorders

ABSTRACT Objective: The purpose of the study was to determine and describe the clinical approach Australian naturopaths take in providing care for people with sleep disorders, as it well known that Australians commonly seek care from naturopathic practitioners and no data on naturopathic practices for people with sleep disorders is currently documented. Participants: Naturopaths registered with the Australian PRACI (Practitioner Research and Collaboration Initiative) involved in the clinical management of people with sleep disorders. Methods: Consenting participants were invited to participate in semi-structured phone interviews conducted with the aid of a project-specific interview guide until data saturation was evident. Interview recordings were transcribed verbatim and analyzed thematically. Results: A total of 20 naturopaths across 5 different Australian states were interviewed, 4 themes were identifiable. Thematic interpretation suggests that (1) sleep health consults were common and involved detailed history taking by providers, (2) herbal remedies supported by lifestyle and sleep hygiene behaviors were the most common treatments recommended, and (3) interprofessional communication to medical doctors was seldom reciprocated. Government-funded research and establishing statutory registration of naturopaths were identified as key steps in profiling the professions role and to develop/evaluate integrated patient-centered sleep health care models. Conclusion: Naturopaths engage in the treatment of sleep disorders yet face barriers in executing an integration of such approaches within the current health care system. Research is required to explore how to overcome these barriers and develop integrated patient-centered models of care within tertiary sleep clinics and/or primary care physicians.
Multi-Level Representation Learning for Chinese Medical Entity Recognition: Model Development and Validation

Background Medical entity recognition is a key technology that supports the development of smart medicine. Existing methods on English medical entity recognition have undergone great development, but their progress in the Chinese language has been slow. Because of limitations due to the complexity of the Chinese language and annotated corpora, these methods are based on simple neural networks, which cannot effectively extract the deep semantic representations of electronic medical records (EMRs) and be used on the scarce medical corpora. We thus developed a new Chinese EMR (CEMR) dataset with six types of entities and proposed a multi-level representation learning model based on Bidirectional Encoder Representation from Transformers (BERT) for Chinese medical entity recognition. Objective This study aimed to improve the performance of the language model by having it learn multi-level representation and recognize Chinese medical entities. Methods In this paper, the pretraining language representation model was investigated; utilizing information not only from the final layer but from intermediate layers was found to affect the performance of the Chinese medical entity recognition task. Therefore, we proposed a multi-level representation learning model for entity recognition in Chinese EMRs. Specifically, we first used the BERT language model to extract semantic representations. Then, the multi-head attention mechanism was leveraged to automatically extract deeper semantic information from each layer. Finally, semantic representations from multi-level representation extraction were utilized as the final semantic context embedding for each token and we used softmax to predict the entity tags. Results The best F1 score reached by the experiment was 82.11% when using the CEMR dataset, and the F1 score when using the CCKS (China Conference on Knowledge Graph and Semantic Computing) 2018 benchmark dataset further increased to 83.18%. Various comparative experiments showed that our proposed method outperforms methods from previous work and performs as a new state-of-the-art method. Conclusions The multi-level representation learning model is proposed as a method to perform the Chinese EMRs entity recognition task. Experiments on two clinical datasets demonstrate the usefulness of using the multi-head attention mechanism to extract multi-level representation as part of the language model.
Immobilization of β‐1,4‐xylanase isolated from Bacillus licheniformis S3

Industrial applications require enzymes to be highly stable and economically viable in terms of reusability. Enzyme immobilization is an exciting alternative to improve the stability of enzymatic processes. Immobilization of β‐1,4‐xylanase produced by Bacillus licheniformis S3 is performed by using two polymer supports (agar–agar and calcium alginate). The maximum enzyme immobilization yield was achieved at a concentration of 3% agar, whereas a combination of sodium alginate, 4%, and calcium chloride, 0.3 M, was used for the formation of immobilized beads. The immobilization process increased the optimum reaction time from 10 min to 35 and 40 min for agar and calcium alginate, respectively, and the incubation temperature increased from 55°C to 60°C for agar, but it remained unchanged for calcium alginate. The pH profile of free and immobilized xylanase was quite similar in both cases. Both the techniques altered the kinetic parameters of immobilized β‐1,4‐xylanase as compared with the free enzyme. The diffusion limit of high molecular weight xylan caused a decline in Vmax of the immobilized enzyme, whereas there was an increase in the Km value. However, calcium alginate‐immobilized enzyme displayed broad thermal stability as compared with agar–agar‐immobilized enzyme and retained 57.1% of its initial activity at 80°C up to 150 min. Biotechnological characterization showed that the reusability of enzymes was the most striking finding, particularly of immobilized xylanase using agar–agar as immobilization carrier, which after six cycles retained 23% activity.
Experimental study on differential diagnosis of cerebral hemorrhagic and ischemic stroke based on microwave measurement

BACKGROUND: Hemorrhagic stroke and ischemic stroke have similar symptoms at the onset of the disease, but their clinical treatment is completely different. The early, effective identification of stroke types can effectively improve the cure rate. OBJECTIVE: In this study, an early, noncontact identification of the stroke type, i.e., hemorrhagic or ischemic, based on a microwave measurement technique was investigated. METHODS: This study was based on animal models of cerebral hemorrhage and cerebral ischemia and the design of a microwave scattering parameter measurement system. RESULTS: The accuracy of the cerebral hemorrhage model with a blood loss interval of 2 ml reached 93.75%. While the accuracy of the cerebral ischemia model with an ischemic interval of 42 minutes reached 91.7%. CONCLUSION: The experimental results show that the system for identifying cerebral stroke based on microwaves can distinguish between cerebral hemorrhage and cerebral ischemia models and effectively distinguish between different degrees of cerebral hemorrhage or different durations of cerebral ischemia. This experimental system is inexpensive, portable, noninvasive, simple, and rapid and thus has good potential as a method for identifying the stroke type prior to hospitalization.
Chronic Treatment with Morphine Disrupts Acute Kinase-Dependent Desensitization of GPCRs

Based on studies using mutations of the µ-opioid receptor (MOR), phosphorylation of multiple sites on the C-terminus has been recognized as a critical step underlying acute desensitization and the development of cellular tolerance. The aim of this study is to explore which kinases mediate desensitization of MOR in brain slices from drug-naïve and morphine-treated animals. Whole-cell recordings from locus coeruleus neurons were made, and the agonist-induced increase in potassium conductance was measured. In slices from naïve animals, pharmacological inhibition of G-protein receptor kinase (GRK2/3) with compound 101 blocked acute desensitization. Following chronic treatment with morphine, compound 101 was less effective at blocking acute desensitization. Compound 101 blocked receptor internalization in tissue from both naïve and morphine-treated animals, suggesting that GRK2/3 remained active. Kinase inhibitors aimed at blocking protein kinase C and c-Jun N-terminal kinase had no effect on desensitization in tissue taken from naïve animals. However, in slices taken from morphine-treated animals, the combination of these blockers along with compound 101 was required to block acute desensitization. Acute desensitization of the potassium conductance induced by the somatostatin receptor was also blocked by compound 101 in slices from naïve but not morphine-treated animals. As was observed with MOR, it was necessary to use the combination of kinase inhibitors to block desensitization of the somatostatin receptor in slices from morphine-treated animals. The results show that chronic treatment with morphine results in a surprising and heterologous adaptation in kinase-dependent desensitization. SIGNIFICANCE STATEMENT The results show that chronic treatment with morphine induced heterologous adaptations in kinase regulation of G protein coupled receptor (GPCR) desensitization. Although the canonical mechanism for acute desensitization through phosphorylation by G protein–coupled receptor kinase is supported in tissue taken from naïve animals, following chronic treatment with morphine, the acute kinase-dependent desensitization of GPCRs is disrupted such that additional kinases, including protein kinase C and c-Jun N-terminal kinase, contribute to desensitization.