Miracle-dz/UniFAR-FTUs huggingface dataset:
Difference gel electrophoresis

DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS‐based methodologies and can circumvent their analytical limitations in areas such as intact protein analysis, (linear) detection over a wide range of protein abundances and, theoretically, applications where extreme sensitivity is needed. Thus, in quantitative proteomics DIGE is usually complementary to MS‐based quantitation and has some distinct advantages. This review describes the basics of DIGE and its unique properties and compares it to MS‐based methods in quantitative protein expression analysis.
Design of a Low Power Low Noise System-on-Chip for ECG Monitoring and Diagnostic

A system-on-chip for the application of Electrocardiogram (ECG) monitoring and diagnostic is proposed in this paper, which includes reference circuit, instrumentation amplifier (IA), band-pass filter (BPF), amplifier stage and successive approximation register analog-to-digital converter (SAR-ADC). The Common-Mode Rejection Ratio (CMRR) of IA is greater than 132.87 dB, total gain of the AFE achieves 62.9 dB, and BPF based on a Small-Gm Operational Transconductance Amplifier (OTA) works in the frequency range of 0.5 Hz to 150 Hz. SAR-ADC achieve the ENOB of 7.5 with low power consumption and decent transition speed. The system with a total power consumption of 334.1uW will be supplied by Lithium-ion battery with a normal voltage at 3.7V, and the total input referred noise is 0.41uV. The fabrication process is AMI 0.6 um. All the schematic designs are simulated in Cadence Virtuoso, and the layout simulation is conducted in Cadence Layout. Index Terms — Current balancing-IA, Small-Gm OTA, Low Drop-out Regulator, SAR-ADC, ECG
Low-cost relative navigation sensors for miniature spacecraft and drones

Proximity relative navigation systems for autonomous small spacecraft and Micro Aerial Vehicles are under continuous investigation, aiming to obtain low-cost and low-resources miniaturized sensors. In this paper, after a review on the state of the art of relative navigation systems for cooperative and non-cooperative miniature space and aerial vehicles, two simple, low cost devices for proximity navigation are presented. The first one is conceived for operation between cooperative vehicles, and exploits a dual system comprising a small camera imaging an array of LEDs and a LED-photodiode couple for precise range estimation based on intensity measurements. The second sensor is designed to perform proximity ranging and attitude determination with respect to non-cooperative objects in space, air or ground. It is based on a miniature camera that detects the light spots on the target surface generated by three lasers mounted on the chaser vehicle. Theoretical models have been developed for both sensors, and were used to perform a preliminary assessment of performance through numerical simulations.
Low-power Parallel Data Processing Using Computation Reuse

A wide range of real-world applications, including DSP, deep learning, multimedia, and scientific algorithms generally include fixed-point and floating-point arithmetic operations and trigonometric functions which have long latency and high power usage. In this paper, we propose a computation reuse mechanism for multicore processors that reuses the results of an arithmetic operation for subsequent operations with (approximately) the same operands. It adds a small so-called result cache to every functional unit that keeps a few recent operands and their results to detect repetitive operands and reuse the results. Taking advantage of the value locality inherent in many real-world applications, our architecture relies on a multi-stage interconnection network to distribute input data elements across the cores of a multi-core processor in such a way that the data locality of each core is increased. This way, each core has higher computation reuse rate that translates to more power consumption reduction. Experimental results show that the proposed mechanism increases the result cache hit rate, which leads to a significant reduction in power consumption of arithmetic operations.
Promotion of callus formation by abscisic Acid in citrus bud cultures.

Abscisic acid is known to control different phases of plant growth processes, and its physiological effects have been recently reviewed (1, 12). Although its inhibitory effects are numerous (1), cases of growth promotion by ABA are scarce (10, 11). Some interactions of ABA with other growth substances, mainly with gibberellic acid, in promoting growth of cucumber hypocotyls (2), and with kinetin, in the growth of soybean callus (3), were also reported. While studying the development of citrus buds in vitro, we noticed a formation of callus in bud cultures which was induced specifically by ABA. The effects of ABA alone, and in combination with IAA and 6-benzylaminopurine are reported.
Recruitment of Tumor Necrosis Factor Receptor-associated Factor Family Proteins to Apoptosis Signal-regulating Kinase 1 Signalosome Is Essential for Oxidative Stress-induced Cell Death*

Apoptosis signal-regulating kinase 1 (ASK1) plays a pivotal role in oxidative stress-induced cell death. Reactive oxygen species disrupt the interaction of ASK1 with its cellular inhibitor thioredoxin and thereby activates ASK1. However, the precise mechanism by which ASK1 freed from thioredoxin undergoes oligomerization-dependent activation has not been fully elucidated. Here we show that endogenous ASK1 constitutively forms a high molecular mass complex including Trx (∼1,500-2,000 kDa), which we designate ASK1 signalosome. Upon H2O2 treatment, the ASK1 signalosome forms a higher molecular mass complex at least in part because of the recruitment of tumor necrosis factor receptor-associated factor 2 (TRAF2) and TRAF6. Consistent with our previous findings that TRAF2 and TRAF6 activate ASK1, H2O2-induced ASK1 activation and cell death were strongly reduced in the cells derived from Traf2-/- and Traf6-/- mice. A novel signaling complex including TRAF2, TRAF6, and ASK1 may thus be the key component in oxidative stress-induced cell death.
Fault diagnosis methodology in smart grid with distributed energy generation

In deployment of smart grid system today's power system infrastructure are changing due to inclusion of large number of distributed generation units which are being integrated into power systems at distribution level. In such a environment of Smart Grid; resilience and self healing property of power system is very crucial. Fault diagnosis plays very important task in achieving these qualities and requires drastic change from its conventional to advanced forms. The proposed technique in this paper aims to locate and isolate a faulty section in a distribution system with distributed generation. The paper proposes the use of latest sensing and communication technology to fit in to automated modernized grid. Multi resolution analysis of fault signal is used to extract feature for fault diagnosis. Simulation is carried out on 15 Bus IEEE system using MATLAB 2013a. Results of developed coding to implement proposed methodology for performing various tasks of fault identification, classification and location are presented. The method can be find useful for locating faults in tomorrow's advanced smart grid environment.
Activation of the Epstein-Barr virus BMRF1 and BZLF1 promoters by ZEBRA in Saccharomyces cerevisiae

ZEBRA has been shown to activate model reporter genes consisting of synthetic oligomerized ZEBRA response elements upstream of a minimal CYC1 promoter fused to beta-galactosidase in the yeast Saccharomyces cerevisiae. Here it is shown that in S. cerevisiae ZEBRA activates transcription of natural Epstein-Barr virus promoters. Two Epstein-Barr virus promoters were shown to be activated by ZEBRA in S. cerevisiae: Zp, the promoter that regulates expression of BZLF1, which encodes ZEBRA; and EAp, the promoter controlling expression of BMRF1, which encodes diffuse early antigen, EA-D. These observations indicate that neither mammalian-specific nor virally encoded coactivators are obligatory for ZEBRA to stimulate expression from these two promoters. Zp was also strongly activated by endogenous yeast factors. EAp was not activated by yeast factors. The results show that in S. cerevisiae and in B cells, ZEBRA dominates the response of EAp; ZEBRA plus endogenous cell factors activate Zp.
Feedback on the Integration of a Serious Game in the Business Process Management Learning

The Business process concept plays a major role in the Information System (IS) domain. Business Process Management (BPM) approaches offer languages, methods and tools for modeling, execution and optimizing business processes. However, these approaches are often difficult to understand by the learners. In particular, the modeling phase often remains abstract, not consensual and complex to implement in real settings. In order to better motivate BPM learners, several BPM's Serious Games (SG) has been developed in recent years. But few studies have evaluated game based educational methods for the BPM learning. This paper presents a feedback on the SG INNOV8 use in BPM teaching. INNOV8's evaluation was conducted within the course: "Modeling and optimization of business processes," proposed to Master students. The feedback from the students and teachers were quite positive. Nevertheless, the study shows a low potential of the game for novice students in this field.
Using Signal Processing Techniques to Enhance the TemperatureMeasurements Using Nanohole Array Sensors

A signal processing approach to reducing noise in the observed transmission through nanohole array sensors used in a microscale calorimeter is presented. The results demonstrate that using rectification and moving average processes, a well-defined Extraordinary Optical Transmission (EOT)-temperature calibration curve can be developed in the presence of a moving light fringe pattern.
Syntheses, structural characterizations, and optical and electrochemical properties of directly fused diporphyrins.

Directly fused diporphyrins display the extensive pi conjugation as evinced by highly perturbed electronic absorption spectra as well as lowered and largely split first oxidation potentials. Such diporphyrins prepared include meso-beta doubly linked diporphyrins 7, meso-meso beta-beta beta-beta triply linked diporphyrins 8, and meso-meso beta-beta doubly linked diporphyrins 9. Oxidation of 5,15-diaryl-substituted and 5,10,15-triaryl-substituted Ni(II)-, Cu(II)-, and Pd(II)-porphyrins with tris(4-bromophenyl)aminium hexachloroantimonate (BAHA) in CHCl(3) afforded 7, and triply linked Cu(II)-diporphyrins 8a and 8g were respectively prepared by the oxidation of meso-meso singly linked Cu(II)-diporphyrins 5c and 5f with BAHA. Meso-meso beta-beta doubly linked Ni(II)-diporphyrin 9a was isolated along with triply linked Ni(II)-diporphyrin 8e from the similar oxidation of meso-meso singly linked Ni(II)-diporphyrin 5a. Doubly linked diporphyrins 7 and 9a both exhibit significantly perturbed electronic absorption spectra, in which the Soret-like bands are largely split at around 405-418 and 500-616 nm and the Q-bandlike absorption bands are substantially intensified and red-shifted at 748-820 nm, probably as a consequence of symmetry lowering. Triply linked diporphyrins 8 display more strongly perturbed electronic absorption spectra with split Soret-like bands at 408-419 and 567-582 nm and Q-bandlike absorption bands reaching far-infrared region. Structures of three types of fused diporphyrins 7b and 7c, 8g and 8j, and 9a have been unambiguously determined by X-ray crystallography to be nearly coplanar. Both the triply linked diporphyrins 8g and 8j exhibit very flat structures, whereas the doubly linked diporphyrins 7b and 7c exhibit ruffled structures. The doubly linked diporphyrin 9a shows a helically twisted conformation with larger ruffling toward the opposite directions and has been actually separated into two enantiomers, which display strong Cotton effects in the CD spectra. The first oxidation potentials (E(OX1)) decrease in the order of 5 > 7 > or = 9 > 8, indicating lift-up of HOMO orbital in this order, and split potential differences DeltaE = E(OX1) - E(OX2), in turn, increase in the reverse order of 5 < 7< or = 9 < 8. The (1)H NMR spectra have indicated that the aromatic porphyrin ring current becomes weakened in the order of 5 > 7 > 8. Collectively, the electronic interactions between the diporphyrins have been concluded to increase in the other of 5 << 7 < or = 9 < 8.
Delta-Sigma Fractional-N Phase-Locked Loops

—This paper presents a tutorial on delta-sigma fractional-N PLLs for frequency synthesis. The presentation assumes the reader has a working knowledge of inte- ger-N PLLs. It builds on this knowledge by introducing the additional concepts required to understand ΔΣ frac- tional-N PLLs. After explaining the limitations of integer-N PLLs with respect to tuning resolution, the paper introduces the delta-sigma fractional-N PLL as a means of avoiding these limitations. It then presents a self-contained explanation of the relevant aspects of delta-sigma modulation, an extension of the well known integer-N PLL linearized model to delta-sigma fractional-N PLLs, a design example, and techniques for wideband digital modulation of the VCO within a delta-sigma fractional-N PLL. 
Isolation and characterization of microsatellite loci from the planarian Dugesia polychroa (Schmidt) (Platyhelminthes: Tricladida)

The genomes of many species have been shown to contain loci composed of tandemly repeated short DNA sequences known as microsatellites or simple sequence motifs (Tautz & Renz 1984). Allelic variation in copy number of the repeat unit renders these loci polymorphic, making them convenient markers for evolutionary studies, including the analysis of population structure (Bruford & Wayne 1993; Schlotterer & Pemberton 1994), mating systems and paternity (Queller et al. 1993). We developed microsatellite markers for the free-living fresh water planarian Dugesia polychroa (Platyhelminthes: Tricladida) to study the population structure and mating system of these simultaneously hermaphroditic worms characterized by internal fertilization. Dugesia polychroa has two reproductive biotypes, sexual diploids and pseudogamous parthenogens, which
New coating system for direct-deposition of sensors on components of arbitrary size: A novel approach allowing for thinner sensors with higher measuring accuracy

A new coating system for the deposition of sensors and thin-layers directly onto components of arbitrary size has been invented at the Institute of Micro Production Technology. This system allows for thinner sensors without any carrier substrate and with a higher measuring accuracy. Within this paper, the basic setup and the functional principle of the new coating system is presented. The deposition process sequence is described and the system is characterized concerning layer homogeneity and evacuation time.
Phase I-II study of high-dose epirubicin in advanced non-small-cell lung cancer.

PURPOSE A phase I multicenter trial was performed to determine the maximum-tolerated dose (MTD) of epirubicin, given on 3 consecutive days every 3 weeks to previously untreated patients with advanced non-small-cell lung cancer (NSCLC). PATIENTS AND METHODS After appropriate staging and a baseline multiple-gated angiogram (MUGA) scan, at least four patients were entered at each dose level, starting at 35 mg/m2 of epirubicin given intravenously (IV) daily for 3 days (105 mg/m2) and escalating by 5 mg/m2 per injection in each dose level (15 mg/m2 per course). Epirubicin was administered up to a maximum dose of 60 mg/m2/d for 3 days (180 mg/m2). The MTD was determined to be 55 mg/m2/d for 3 days (165 mg/m2) after treating a total of 35 (33 assessable) patients. Nadir granulocyte counts and associated febrile episodes comprised the dose-limiting toxicity, but there were no treatment-related deaths. A phase II trial was performed using a dose of 50 mg/m2/d for 3 days (150 mg/m2) every 3 weeks with no dose escalation, but with dose reduction for toxicity as required. A total of 30 patients were entered onto this phase of the study. RESULTS The major toxicity, as in the phase I trial, was neutropenia with five febrile episodes, again with no treatment-related deaths. An overall response rate of 12 of 63 (19%) was noted in the combined patient population of the phase I-II trial, with 95% confidence intervals of 10% to 31%. When the response rate was analyzed by histology, only one of 17 (6%) patients with squamous histology, as compared with 11 of 46 (24%) with non-squamous histology, responded, but this did not reach statistical significance (P = .15). CONCLUSIONS High-dose epirubicin is tolerable and is an active single agent in NSCLC. It should be combined with relatively nonmyelosuppressive agents such as cisplatin to try to obtain higher response rates and extend the survival in this disease.
Adrenocorticotropin concentration following administration of thyrotropin-releasing hormone in healthy horses and those with pituitary pars intermedia dysfunction and pituitary gland hyperplasia.

OBJECTIVE To compare the effect of thyrotropin-releasing hormone (TRH) administration on endogenous ACTH concentrations in healthy horses and those with pituitary pars inter-media hyperplasia and compare the test with the dexamethasone suppression test (DST). DESIGN Prospective case series. ANIMALS 15 horses with clinical signs of pituitary pars intermedia dysfunction (PPID), 4 horses with equivocal signs of PPID, and 29 horses without signs of PPID. PROCEDURES ACTH concentrations prior to and after administration of TRH were measured 61 times in 48 horses. Results of the DST (cortisol response) were compared with those of the TRH test in 29 horses. Thirty-three horses (24 with no clinical signs of PPID, 5 with clinical signs of PPID, and 4 with equivocal clinical signs of PPID) were euthanized and necropsied and their pituitary glands evaluated. RESULTS ACTH concentrations increased in all horses, but magnitude and duration of increase were significantly higher in horses with PPID. Endogenous ACTH concentrations were influenced by season. The ACTH baseline concentrations and response to TRH were not correlated with results of the DST. Results of DST were abnormal only in clinically abnormal horses or those with pars intermedia hyperplasia, but were within reference range in 17 of 26 tests in these horses. CONCLUSIONS AND CLINICAL RELEVANCE The ACTH response to TRH is a useful test for diagnosis of pituitary gland hyperplasia, particularly in horses in which baseline ACTH concentrations are within reference range. The DST was specific but not sensitive and was inconsistent for individuals, and results often did not agree with the TRH test response.
Nucleic Acids Research

Two new endonuclease activities, endonuclease B and endonuclease C, obtained from yeast nuclear preparations have been separated and partially characterized. Endonuclease B has a primary requirement for Mn which cannot be replaced by Mg or Ca , and makes single-strand scissions in 2+ 2+ double-stranded DNA. Endonuclease C is activated by either Mn or Mg , and makes single-strand scissions with Mg , while with Mn , scissions are made which result in double-strand breaks. Neither enzyme is active on denatured DNA, and both are inhibited by yeast RNA. Both enzymes exhibit pH optima at pH 5.0 and pH 7.2, and leave 5'-phosphoryl termini.
Dopamine and dihydroergocryptine binding to the anterior pituitary and other brain areas of the rat and sheep.

Subcellular preparations of the anterior pituitary of rats and sheep bind [3H]dopamine ([3H]DA) and the dopaminergic agonist [3H]dihydroergocryptine ([3H]DHE) in a manner compatible with interactions at a DA receptor. The binding is high affinity ([3H]DA, dissociation constant = 65 nM; [3H]DHE, dissociation constant = 5 nM), low capacity ([3H]DA, 336 fmol/mg protein; [3H]DHE, 250 fmol/mg protein) and seems to be associated with a single order of binding sites. In addition, the binding is stereoselective, as the physiologically active (+)isomer of butaclamol reduces binding of [3H]DA and [3H]DHE by 50% at concentrations 1000 times less than the inactive (-)butaclamol. Interactions of dopaminergic and adrenergic agonists and antagonists with this binding site mimic their pharmacological potencies at a DA receptor. A similar binding site was present in the corpus striatum and the posterior pituitary, regions which contain DA axon terminals, as well as in the region of the median eminence. However, insufficie.
A New Approach to the Measurement of Quality of Life: The Patient‐Generated Index

Quality of life has been defined as “the extent to which our hopes and ambitions are matched by experience. ” To improve a patient's quality of life through medical care would be to “narrow the gap between a patient's hopes and expectations and what actually happens. ” Using the above definition as a conceptual basis, we produced a self-administered, Patient-Generated Index (PGI) of quality of life. The PGI was completed by 359 patients presenting with low back pain. The validity of the measure was assessed by correlating patients' PGI scores with a well-validated health profile, the Short-Form 36-item Health Survey (SF-36), and with their scores on a clinical back pain questionnaire. Stepwise multiple regression was then used to model the relationship between the PGI score and the SF-36. Patients' PGI scores showed a high correlation with SF-36 scales measuring pain, social functioning, and role limitations attributable to physical problems, and with the clinical questionnaire. Together with whether a person was retired or not, these health variables were able to explain 25% of the variance in PGI scores. Patient generated index scores were significantly lower in patients referred to hospital compared with those managed solely in general practice and tended to reflect the general practitioner's assessment of symptom severity. We conclude that it is possible to construct a questionnaire that quantifies the effect of a medical condition on patients' quality of life in a way that has meaning and relevance in the context of their daily lives. The PGI has considerable potential for routine use in a wide range of clinical conditions for which the measurement of outcome has hitherto proved very difficult.
Input Parameters for the Simulation of Silicon Solar Cells in 2014

Within the silicon photovoltaics (PV) community, there are many approaches, tools, and input parameters for simulating solar cells, making it difficult for newcomers to establish a complete and representative starting point and imposing high requirements on experts to tediously state all assumptions and inputs for replication. In this review, we address these problems by providing complete and representative input parameter sets to simulate six major types of crystalline silicon solar cells. Where possible, the inputs are justified and up-to-date for the respective cell types, and they produce representative measurable cell characteristics. Details of the modeling approaches that can replicate the simulations are presented as well. The input parameters listed here provide a sensible and consistent reference point for researchers on which to base their refinements and extensions.
WHOIS Protocol Specification

This document updates the specification of the WHOIS protocol, thereby obsoleting RFC 954. The update is intended to remove the material from RFC 954 that does not have to do with the on-the-wire protocol, and is no longer applicable in today's Internet. This document does not attempt to change or update the protocol per se, or document other uses of the protocol that have come into existence since the publication of RFC 954. [STANDARDS-TRACK]