Miracle-dz/UniFAR-FTUs huggingface dataset:
FlyORF-TaDa allows rapid generation of new lines for in vivo cell-type-specific profiling of protein–DNA interactions in Drosophila melanogaster

Abstract Targeted DamID (TaDa) is an increasingly popular method of generating cell-type-specific DNA-binding profiles in vivo. Although sensitive and versatile, TaDa requires the generation of new transgenic fly lines for every protein that is profiled, which is both time-consuming and costly. Here, we describe the FlyORF-TaDa system for converting an existing FlyORF library of inducible open reading frames (ORFs) to TaDa lines via a genetic cross, with recombinant progeny easily identifiable by eye color. Profiling the binding of the H3K36me3-associated chromatin protein MRG15 in larval neural stem cells using both FlyORF-TaDa and conventional TaDa demonstrates that new lines generated using this system provide accurate and highly reproducible DamID-binding profiles. Our data further show that MRG15 binds to a subset of active chromatin domains in vivo. Courtesy of the large coverage of the FlyORF library, the FlyORF-TaDa system enables the easy creation of TaDa lines for 74% of all transcription factors and chromatin-modifying proteins within the Drosophila genome.
TLR2-mediated activation of innate responses in the upper airways confers antiviral protection of the lungs

The impact of respiratory virus infections on global health is felt not just during a pandemic, but endemic seasonal infections pose an equal and ongoing risk of severe disease. Moreover, vaccines and antiviral drugs are not always effective or available for many respiratory viruses. We investigated how induction of effective and appropriate antigen-independent innate immunity in the upper airways can prevent the spread of respiratory virus infection to the vulnerable lower airways. Activation of TLR2, when restricted to the nasal turbinates, resulted in prompt induction of innate immune–driven antiviral responses through action of cytokines, chemokines, and cellular activity in the upper but not the lower airways. We have defined how nasal epithelial cells and recruitment of macrophages work in concert and play pivotal roles to limit progression of influenza virus to the lungs and sustain protection for up to 7 days. These results reveal underlying mechanisms of how control of viral infection in the upper airways can occur and support the implementation of strategies that can activate TLR2 in nasal passages to provide rapid protection, especially for at-risk populations, against severe respiratory infection when vaccines and antiviral drugs are not always effective or available.
Behavioral Responses of Wild Rodents to Owl Calls in an Austral Temperate Forest

Simple Summary Growing human populations are challenging scientists to find effective ways to control and mitigate human–wildlife conflict while preserving biodiversity. It has been reported that predator odor and calls can drive away rodents, but little is known about species-specific responses of prey. For these reasons, we compared the behavioral changes of common rodent species inhabiting the Chilean temperate forest (Abrothrix spp., the long-tailed pygmy rice rat Oligoryzomys longicaudatus and the black rat Rattus rattus) when exposed to two different native predator calls (the austral pygmy owl Glaucidium nana and the rufous-legged owl Strix rufipes) and a control (no predator calls). Our results showed that all rodent species modified their behavior in the presence of predator calls, but the effects were species dependent. These findings point to the need to carefully study target rodent species instead of applying a general control plan for all rodent species. Abstract Ecologically based rodent management strategies are arising as a sustainable approach to rodent control, allowing us to preserve biodiversity while safeguarding human economic activities. Despite predator signals being known to generally repel rodents, few field-based studies have compared the behavioral effects of several predators on different prey species, especially in Neotropical ecosystems. Here, we used camera traps to study the behavior of rodent species native to the Chilean temperate forest (Abrothrix spp., long-tailed pygmy rice rat Oligoryzomys longicaudatus) and an introduced rodent (black rat Rattus rattus). Using playbacks of raptor calls, we experimentally exposed rodents to three predation risk treatments: austral pygmy owl calls (Glaucidium nana), rufous-legged owl calls (Strix rufipes) and a control treatment (absence of owl calls). We evaluated the effects of the treatments on the time allocated to three behaviors: feeding time, locomotor activity and vigilance. Moonlight and vegetation cover were also considered in the analyses, as they can modify perceived predation risk. Results showed that predator calls and environmental factors modified prey behavior depending not only on the predator species, but also on the rodent species. Consequently, owl playbacks could be regarded as a promising rodent control tool, knowing that future studies would be critical to deeply understand differences between species in order to select the most effective predator cues.
Interfaces as design tools for the InAs/GaSb/InSb short-period superlattice for mid-infrared emission

We use a standard 8 × 8 envelope-function approximation (EFA) formalism taking into account the effect of anisotropic and other interface (IF) interactions to investigate the electronic and optical properties of short-period superlattice laser structures (InAs/GaSb/InSb)×N grown on a GaSb substrate. We find that the band gaps numerically calculated at different temperatures give a good fitting with the experimental data confirming the model used. The calculated modal gain demonstrates that it is possible to achieve lasing operation at room temperature for N > 12 and for a reasonable total optical loss αt = 25 cm−1. Therefore, the 8 × 8 EFA formalism with IF design serves as a tool to model the optoelectronic properties of InAs/GaSb/InSb SPSLs.
Engineering Three-Dimensional Vascularized Cardiac Tissues.

Heart disease is one of the largest burdens to human health worldwide and has very limited therapeutic options. Engineered three-dimensional (3D) vascularized cardiac tissues have shown promise in rescuing cardiac function in diseased hearts and may serve as a whole organ replacement in the future. One of the major obstacles in reconstructing these thick myocardial tissues to a clinically applicable scale is the integration of functional vascular networks capable of providing oxygen and nutrients throughout whole engineered constructs. Without perfusion of oxygen and nutrient flow throughout the entire engineered tissue, not only is tissue viability compromised, but overall tissue functionality is lost. There are many supporting technologies and approaches that have been developed to create vascular networks such as 3D bioprinting, co-culturing hydrogels, and incorporation of soluble angiogenic factors. In this state-of-the-art review we discuss some of the most current engineered vascular cardiac tissues reported in the literature and future directions in the field.
Amphiregulin Regulates Melanocytic Senescence

Oncogene-induced senescence (OIS) is a decisive process to suppress tumor development, but the molecular details of OIS are still under investigation. Using an established OIS model of primary melanocytes transduced with BRAF V600E and compared to control cells, amphiregulin (AREG) was shown to be induced. In addition, AREG expression was observed in nevi, which by definition, are senescent cell clusters, compared to melanocytes. Interestingly, treatment of melanocytes with recombinant AREG did induce senescence. This led to the assumption that extracellular AREG has an important function in this process. Inhibition of the epidermal growth factor receptor (EGFR) using Gefitinib identified AREG as one of EGFR ligands responsible for senescence. Furthermore, depletion of AREG expression in senescent BRAF V600E melanocytes resulted in a significant reduction of senescent melanocytes. This study reveals AREG as an essential molecular component of signaling pathways leading to senescence in melanocytes.
Do fear of COVID-19 and religious coping predict depression, anxiety, and stress among the Arab population during health crisis?

Abstract The roles of fear of COVID-19 and religious coping were examined in relation to depression, anxiety, and stress. Participants (N = 259; 88% female; M age = 32.96, SD = 8.88) completed measures of fear of COVID-19, religious coping, depression, anxiety, and stress. Results showed that fear of COVID-19 was associated with depression, anxiety, and stress. Negative religious coping was associated with depression, anxiety, and stress while positive religious coping was only associated with depression and stress. Fear of COVID-19 and negative religious coping may be detrimental to mental health while positive religious coping may reduce depression and stress.
Enzyme-Assisted Release of Antioxidant Peptides from Porphyra dioica Conchocelis

The conchocelis life cycle stage of P. dioica represents an unexplored source of bioactive compounds. The aim of this study was to generate and characterise, for the first time, hydrolysates of conchocelis using a specific combination of proteases (Prolyve® and Flavourzyme®). Hydrolysate molecular mass distribution and free amino acid contents were assessed, and the antioxidant activity was determined using a range of in vitro assays. The protein content and the total amino acid profiles of conchocelis were also studied. Conchocelis contained ~25% of protein (dry weight basis) and had a complete profile of essential amino acids. Direct sequential enzymatic treatment modified the profile of the generated compounds, increasing the amount of low molecular weight peptides (<1 kDa). There was a significant improvement in the antioxidant activity of the hydrolysates compared with the control (up to 2.5-fold), indicating their potential as a novel source of antioxidant ingredients.
Gastric emptying of solutions containing the natural sweetener erythritol and effects on gut hormone secretion in humans: A pilot dose‐ranging study

To determine whether a dose‐dependent effect in the stimulation of gut hormone release (plasma cholecystokinin [CCK], active glucagon‐like peptide‐1 [aGLP‐1] and peptide tyrosine tyrosine [PYY]) is found for the natural sweetener erythritol.
Acute Effects of a Single Bout of Walking on Affective Responses in Patients with Major Depressive Disorder

Exercise programs are considered an effective (add-on) treatment option for depressive disorders. However, little is known about the acute effects of exercise on affective responses in in-patient settings. Therefore, the aim of the present study was to compare the effects of a single 30-min bout of walking on affective responses to a passive control condition in patients with major depressive disorder during treatment in a mental health center. In total, 23 in-patients were exposed to two conditions (duration: 30 min) using a within-subject design: an exercise (light–moderate walking outdoors) and a passive control condition (sitting and reading). Affective responses were assessed based on the Circumplex Model in four phases: pre, during, post, and two hours following the conditions. The main results include a significantly larger pre–post increase in energetic arousal in the exercise condition compared to the control condition, p = 0.012, ηp² = 0.25, but no significantly different pre–follow-up change between conditions, p = 0.093, ηp² = 0.12. Negatively valenced affective responses showed significantly stronger pre–post decreases after the exercise condition compared to the passive control condition, p < 0.036, ηp² > 0.18. Positively valenced affective response activity showed a pre–post increase in the exercise condition and a pre–post decrease in the passive control condition, p = 0.017, ηp² = 0.23. The higher-activated, positively valenced immediate response of light- to moderate-intensity walking may serve as an acute emotion regulation in patients with major depressive disorder and provide a favorable state for adherence to exercise programs.
Persistence and Potential Lethality in Intimate Partner Violence: Evaluating the Concurrent and Predictive Validity of a Dual Risk Assessment Protocol

This study seeks to determine the concurrent and predictive validity of a dual risk assessment protocol. It combines the risk of persistence in intimate partner violence (IPV) measured via the Domestic Violence Screening Instrument–Revised (DVSI-R) with supplemental items from the Danger Risk Assessment (DRA) bearing on the risk of potential lethality. We further test whether this assessment protocol reproduces disparities by race and ethnicity found in the larger population. Using a sample of 4,665 IPV male defendants with a female victim, analyses support both types of criterion validity. The DRA risk score is associated with felony charges, incarceration at the initial arrest, and the frequency of subsequent dangerous behavior. Results also suggest minimal predictive bias or disparate impact by race and ethnicity. Incorporating supplemental items bearing on potential lethality risk adds important information concerning the risk management strategies of those involved in IPV.
Animal models for cystic fibrosis: a systematic search and mapping review of the literature. Part 2: nongenetic models

Various animal models are available to study cystic fibrosis (CF). These models may help to enhance our understanding of the pathology and contribute to the development of new treatments. We systematically searched all publications on CF animal models. Because of the large number of models retrieved, we split this mapping review into two parts. Previously, we presented the genetic CF animal models. In this paper we present the nongenetic CF animal models. While genetic animal models may, in theory, be preferable for genetic diseases, the phenotype of a genetic model does not automatically resemble human disease. Depending on the research question, other animal models may thus be more informative. We searched Pubmed and Embase and identified 12,303 unique publications (after duplicate removal). All references were screened for inclusion by two independent reviewers. The genetic animal models for CF (from 636 publications) were previously described. The non-genetic CF models (from 189 publications) are described in this paper, grouped by model type: infection-based, pharmacological, administration of human materials, xenografts and other. As before for the genetic models, an overview of basic model characteristics and outcome measures is provided. This CF animal model overview can be the basis for an objective, evidence-based model choice for specific research questions. Besides, it can help to retrieve relevant background literature on outcome measures of interest.
Modelling polymers with side chains: MEH-PPV and P3HT

Modelling polymers with side chains is always a challenge once the degrees of freedom are very high. In this study, we present a successful methodology to model poly[2-methoxy-5-(2′-ethyl-hexyloxy)-p-phenylenevinylene] (MEH-PPV) and poly[3-hexylthiophene] (P3HT) in solutions, taking into account the influence of side chains on the polymer conformation. Molecular dynamics and semi-empirical quantum mechanical methods were used for structure optimisation and evaluation of optical properties. The methodology allows to describe structural and optical characteristics of the polymers in a satisfactory way, as well as to evaluate some usual simplifications adopted for modelling these systems. Effective conjugation lengths of 8-14.6 and 21 monomers were obtained for MEH-PPV and P3HT, respectively, in accordance with experimental findings. In addition, anti/syn conformations of these polymers could be predicted based on intrinsic interactions of the lateral branches.
Rehearsal and Event Age Predict the Fading Affect Bias across Young Adults and Elderly in Self-Defining and Everyday Autobiographical Memories

ABSTRACT The faster fading of unpleasant affect than pleasant affect is known as the Fading Affect Bias (FAB). The FAB generalizes across cultures and event types, it is positively related to rehearsals and healthy outcomes, and it is negatively related to unhealthy outcomes. Experiment 1 Objective, Sample/Population, and Method: We examined the importance of different rehearsal types for the FAB across self-defining and everyday events in 58 college age participants using a self-guided questionnaire procedure in Experiment 1. Experiment 1 Results: We found robust FAB effects across event types, FAB increased with both event age and event sharing (number of people), and rehearsals mediated these relations. Moreover, event sharing and talking about the event combined to predict the FAB. Experiment 2 Objective, Sample/Population, and Method: In Experiment 2, we used the self-guided questionnaire procedure from Experiment 1 for 31 college students and 12 elderly participants 68 to 84 years old, as well as an interview procedure with 13 elderly participants 68 to 94 years old. Experiment 2 Results: We combined the elderly data because both groups showed similar FAB patterns. We found robust FAB effects across both event types, the FAB increased with event age and participant age, and it increased with talking rehearsals. Conclusions: The results extend the FAB to self-defining events and the elderly, they emphasize the importance of various rehearsal types, and they are in line with FAB research, age research, and research on several emotion regulation models.
Molecular and morphological analyses reveal new taxa additions to the tribe Streblocladieae (Rhodomelaceae, Rhodophyta)

The recent segregation of 12 genera in the tribe Streblocladieae suggests that the taxonomy of some species belonging to Polysiphonia sensu lato is updated with the transfer and the proposal of new combinations. Accordingly, six new additions to the tribe Streblocladieae on the basis of morphological and molecular analyses are presented as a consequence of this new segregation. These additions include the description of the new species Carradoriella platensis sp. nov., the proposal of the following new combinations Eutrichosiphonia paniculata comb. nov., E. tapinocarpa comb. nov., and the reinstatement of Vertebrata curta, V. decipiens, and V. patersonis. Additionally, our morphological observations identified additional diagnostic features for two genera of the Streblocladieae. Carradoriella has branches with sexual reproductive structures arranged adaxially on branchlets, and the recently described Eutrichosiphonia has rhizoids with multicellular digitate haptera. Our study gives insights in regards to the distribution, the diagnostic features for delimiting genera morphologically, and the molecular evolutionary relationships in the Streblocladieae.
Neural Antibody Testing for Autoimmune Encephalitis: A Canadian Single-Centre Experience

ABSTRACT: Neural antibodies have emerged as useful biomarkers in suspected autoimmune encephalitis. We reviewed results of neural antibody testing (anti-N-methyl D-aspartate receptor (NMDAR), leucine-rich glioma-inactivated protein (LGI1), contactin-associated protein-like 2 (CASPR2), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR), γ-aminobutyric acid type B receptor (GABA(B)R), dipeptidyl-peptidase-like protein-6 (DPPX), IgLON family member 5 (IgLON5) and glutamic acid decarboxylase-65 (GAD65)) using cell-based assays (CBAs) and tissue indirect immunofluorescence (TIIF) at our centre. Our findings suggest increased clinical sensitivity of CBA compared to TIIF. However, this may come at some expense to clinical specificity, as evidenced by possible false-positive results when weak serum positivity by CBA was observed for certain antibodies (i.e. anti-NMDAR, CASPR2). In such cases, correlation with serum TIIF, as well as CSF CBA and TIIF, aids in identifying true-positive results.
Long term SARS-CoV-2 infectiousness among three immunocompromised patients: from prolonged viral shedding to SARS-CoV-2 superinfection

Abstract Guidelines for stopping COVID-19 patient isolation are mainly symptom-based, with isolation for 10 to 20 days depending on their condition. Here, we describe three deeply immunocompromised patients, each with different clinical evolutions. Asymptomatic carriage, symptom resolution, or superinfection with a second SARS-CoV-2 strain were observed, all leading to prolonged infectious viral shedding several months. We followed the patients epidemiological, clinical, serological data, infectiousness using viral culture and viral mutations accumulated over time. Understanding underlying mechanisms and frequency of prolonged infectiousness is crucial to adapt current guidelines and strengthen the use of systematic PCR testing before stopping isolation in immunocompromised populations.
Midkine: Utility as a Predictor of Early Diabetic Nephropathy in Children with Type 1 Diabetes Mellitus

Objective: This study aimed to assess the role of serum midkine (MK) as a biomarker for early detection of diabetic nephropathy in children with type 1 diabetes mellitus (T1DM) before microalbuminuria emerges. Methods: A total of 120 children with T1DM, comprising 60 microalbuminuric patients (Group 1), 60 normoalbuminuric patients (Group 2), and 60 healthy participants as a control group (Group 3) were included. Detailed medical history, clinical examination, and laboratory assessment of high-sensitivity C-reactive protein (hs-CRP), hemoglobin A1c percentage (HbA1c%), lipid profile, urinary albumin to creatinine ratio (ACR), serum MK and estimated glomerular filtration rate based on serum creatinine were performed in all participants. Results: Both Group 1 and Group 2 had significantly higher serum MK compared to controls (p<0.001). Additionally, significantly higher MK concentrations were present in Group 1 compared with Group 2 (p<0.001). Receiver operating characteristic curve analysis revealed that the MK concentration cutoff value of 1512 pg/mL was able to predict microalbuminuria with a sensitivity of 96% and specificity of 92%. Stepwise regression analysis revealed that HbA1c%, hs-CRP, and ACR were independently related to MK levels (p<0.001 for each). Conclusion: The results of this study suggest that serum MK is a useful, novel, practical marker for the evaluation of renal involvement in children with T1DM, especially in normoalbuminuric children.