Miracle-dz/UniFAR-FTUs huggingface dataset:
Interferon-alpha enhances cross-presentation in human dendritic cells by modulating antigen survival, endocytic routing and processing.

Cross-presentation allows antigen-presenting cells to present exogenous antigens to CD8 + T cells, playing an essential role in controlling infections and tumour development. Interferon-alpha induces the rapid differentiation of human monocytes into dendritic cells, known as IFN-DC, highly efficient in mediating cross-presentation, as well as the cross-priming of CD8 + T cells. In the present study, we have investigated the mechanisms underlying the cross-presentation ability of IFN-DC, by studying the intracellular sorting of soluble ovalbumin and non structural-3 protein of Hepatitis C virus. Our results demonstrate that, independently from the route and mechanism of antigen entry, IFN-DC are extraordinarily competent in preserving internalised proteins from early degradation and in routing antigens toward the MHC-I processing pathway, allowing long-lasting cross-priming capacity. In IFN-DC both early and recycling endosomes function as key compartments for the storage of both antigens and MHC class-I molecules and for proteasome- and TAP-1-dependent auxiliary cross-presentation pathways. As IFN-DC closely resemble human DC naturally occurring in vivo in response to infections and other danger signals, these findings may have important implications for the design of vaccination strategies in neoplastic or chronic infectious diseases. processing pathway, ii) the maturation kinetic of the organelles containing the internalised proteins, iii) the Ag stability within endosomes, and iv) the Ag processing and cross-presentation to specific CD8 + T cells. The results reveal that IFN-DC exhibit a delayed endosomal acidification associated with a prolonged Ag survival and retention in the early-endosomal compartment, as well as with Ag trafficking to recycling pathways. In IFN-DC both early and recycling endosomal compartments serve as important stores of MHC-I molecules, allowing rapid presentation of exogenous Ags. These findings provide novel mechanistic insights into the cross-presentation efficiency of IFN-DC and underscore the potential advantage of using these cellular adjuvants for therapeutic vaccination of patients with neoplastic or chronic infectious diseases. 488, 594 and 633 nm and using the Leica Confocal Software (Leica Lasertechnik) and Adobe Photoshop software programs (Adobe system Incorporated). Several cells were analysed for each labelling condition and representative results are presented.
of an alveolar cell of origin to the high-grade malignant phenotype of pregnancy-associated breast cancer.

Pregnancy-associated breast cancers (PABCs) are malignancies diagnosed during pregnancy or up to five years following parturition, and are usually aggressive, stroma-rich, and estrogen receptor/ progesterone receptor-negative; but little is known about the cellular origin of PABCs or the mechanisms by which PABCs initiate. Using the RCAS retrovirus to deliver the ErbB2 oncogene into the mammary epithelium of our previous reported MMTV- tva transgenic mice, we detected human PABC-like tumors during pregnancy and lactation but not in involuted mice or in age-matched virgin mice. More importantly, by generating a WAP- tva transgenic line for expression of ErbB2 selectively in WAP + mammary alveolar cells, we found that the resulting tumors exhibited the hallmarks of PABCs irrespective of the time since pregnancy and even in the absence of pregnancy. These data suggest that PABCs arise preferentially from an alveolar cell population that expands during pregnancy and lactation. This somatic mouse model may also be useful for preclinical testing of new prophylactic and therapeutic strategies against PABC.
University of Birmingham Law of large numbers for the largest component in a hyperbolic model of complex networks

We consider the component structure of a recent model of random graphs on the hyperbolic plane that was introduced by Krioukov et al. The model exhibits a power law degree sequence, small distances and clustering, features that are associated with so-called complex networks. The model is controlled by two parameters α and ν where, roughly speaking, α controls the exponent of the power law and ν controls the average degree. Reﬁning earlier results, we are able to show a law of large numbers for the largest component. That is, we show that the fraction of points in the largest component tends in probability to a constant c that depends only on α, ν , while all other components are sublinear. We also study how c depends on α, ν . To deduce our results, we introduce a local approximation of the random graph by a continuum percolation model on R 2 that may be of independent interest.
PREDICTING EXACERBATIONS IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

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Mapping of the Inducible I (cid:107) B Phosphorylation Sites That Signal Its Ubiquitination and Degradation†

Extracellular stimuli that activate the transcription factor NF- (cid:107) B cause rapid phosphorylation of the I (cid:107) B (cid:97) inhibitor, which retains NF- (cid:107) B in the cytoplasm of nonstimulated cells. Phosphorylation of I (cid:107) B (cid:97) is followed by its rapid degradation, the inhibition of which prevents NF- (cid:107) B activation. To determine the relationship between these events, we mapped the inducible phosphorylation sites of I (cid:107) B (cid:97) . We found that two residues, serines 32 and 36, were phosphorylated in response to either tumor necrosis factor, interleukin-1, or phorbol ester. Substitution of either serine blocks or slows down induction of I (cid:107) B (cid:97) degradation. Substitutions of the homologous sites in I (cid:107) B (cid:98) , serines 19 and 23, also prevent inducible I (cid:107) B (cid:98) degradation. We suggest that activation of a single I (cid:107) B kinase or closely related I (cid:107)
Entropy Based Feature Extraction for Power Quality Disturbances

: Power line disturbances are mainly caused due to voltage sag, voltage swell, outage and harmonics. These disturbances must be recognized and classified. This paper presents wavelet based decomposition of the disturbances using norm entropy and pattern recognition by probabilistic neural network. In this paper total of 175 power quality disturbance signals are considered for designing the neural network model.
Critical Evaluation on the Importance of Aspects regarding Human Resource Development for Organization Strip

– The fundamental aim of HRD is to improve the resource capability of an organization in a way that it will lead to the attainment of optimum level of work performance. Thus, HRD seeks to develop employees in order to improve their competences that will lead to the improvement in organizational performance, and thus contributing to the achievement of competitive advantage. In this article, three aspects namely Learning Styles, Training Needs Analysis and Training Evaluation are investigated critically in order to shed light of their significances in Human Resource Development for Organization strip and achieve competitive advantage in an organization. Also, a new framework in an effective HRD system based on the works of literature is proposed. Besides, a comparison points our proposal in respect of the state of art in HRD system aspects is conducted. The results pointed out three aspects as crucial elements in the HRD system that can be deployed successfully in the industrial domain to enhance organizational effectiveness.
Estimating Prediction Uncertainty for CNN-based Visual Odometry

—Recently, we have witnessed a considerable diffusion of Convolutional Neural Network approaches for Visual Odome-try applications. However, most of them do not provide any kind of information about the uncertainty of their estimates, which is crucial to effectively integrate them into robotic navigation systems. Inspired by these considerations, we propose Uncertainty-aware Visual Odometry (UA-VO), a novel CNN architecture that computes relative pose predictions and provides uncertainty measures about those estimations.
NDT_A_248453 1653..1659

1Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3The Affiliated Brain Hospital of Guangzhou Medical University (Guangzhou Huiai Hospital), Guangzhou, People’s Republic of China; 4Guangdong Engineering Technology Research Center for Translational Medicine of Mental Disorders, Guangzhou, People’s Republic of China; 5Center for Excellence in Brain Science and Intelligence Technology (CEBSIT), Chinese Academy of Sciences, Shanghai, People’s Republic of China; 6Brain Science and Technology Research Center, Shanghai Jiao Tong University, Shanghai, People’s Republic of China
ANTIMICROBIAL SUSCEPTIBILITY TO SALMONELLA AND E.COLI ISOLATES ORIGINATED FROM BROILER CHICKENS IN LUXOR GOVERNORATE

Salmonellosis and colibacillosis are continuing serious problems facing poultry industry in Egypt. In this study, 300 different pooled broiler chickens organs samples as (trachea, lung, liver, heart, spleen, unabsorbed yolk sac and intestine) were collected from different apparently healthy and sick broiler flocks in different localities of Luxor governorate during (2015 and 2016). The examined broiler flocks were suffering from various health problems developed during the final two weeks of the growing period, resulting in increased mortality and condemnation losses. Bacterial isolation was done by using standard method of isolation and identification. The results showed that 92 out of 300 broiler organs samples were positive for Salmonella spp. (30.66%) while 161 out of 300 were E.coli positive (53.66%). The present study showed that the main Salmonella spp. isolates were ( S . Typhimurium (24%), the both of S . Enteritidis, S . Anatum were (21.7%) and S . Kentucky (19.56%), S . Bargny and S . Molade (3.26%) then S . Newport, S. Ingada and S . Agona their percentage were (2.17%) respectively, while 8 serotypes of E.coli were obtained with the following serological identification O78 (44%), (17.39%), O91:H21 (15.52%), O128:H2 (13%) and other E.coli serotypes were identified as (O2:H6, O26:H11, O55:H7, O146:H21) their percentage was (1:3%). Bacterial strains were tested against 21 antibacterial agents using the standard disk diffusion method on Muller and Hinton’s Agar medium. The results were recorded that most of Salmonella spp were highly resistant to (Oxytetracycline, Doxycycline, Tetracycline then Enrofloxacin, Sulphamethoxazole) and were sensitive to (Gentamycin, colistinsulphate and Ceftiofur). While most of E.coli isolates were resistant to Neomycin and Streptomycin and were sensitive to Ceftiofur then Colistinsulphate.
Get Give Make Live An empirical comparative study of motivations for technology, youth and arts entrepreneurship

Purpose – This study aims to explore the motivations and business ideation processes of 776 entrepreneurs from threediverse cohorts oftechnology, youthandarts entrepreneurs. Design/methodology/approach – Using an inductive approach inspired by grounded theory, observations resultant from the use of a Web-based digital test environment are openly coded, in which 776 individual entrepreneurs have stated their objectives for engaging in entrepreneurship and performed a businessideationprocess. Findings – The study inductively derives a typology of objectives types – “ GET GIVE MAKE LIVE ” – and ﬁ nds that beyond the pursuit of pro ﬁ table opportunities, there is considerable variation, complexity and combinations to the reasons why individuals engage in entrepreneurship. A total of 76 percent of the population in this study have more than one objective, with 48% having more than one type of objective. While the arts entrepreneurs tended to engage in entrepreneurship to “ LIVE ” and the tech entrepreneurs were more inclined to “ GET, ” the most frequently observed objective type in all cohorts was to “ MAKE. ” A total of 74 percent of the entrepreneurs took an effectual approach and began de ﬁ ning their business idea with their core competency,yet technologyentrepreneurswere the most likely tostartby de ﬁ ning their key market. Practical implications – Entrepreneurship educators, trainers and helpers should refrain from a standardized approach which assumes that entrepreneurs share the same set of singular motivations. Interventions might bene ﬁ t from a student-centered program which promotes re ﬂ ection and articulation of theentrepreneurs ’ objectives andtheirdiversity. Originality/value – This study answers the call for research to embrace entrepreneurial diversity and compliment previous explorations of entrepreneurs ’ motivations through an empirically grounded study of threediverse cohorts of entrepreneurs.
Social Media Analytics in Support of Documentary Production

—Recent market research has revealed a globally growing interest on documentaries that have now become one of the most populated content-wise genre in the movie titles catalog, surpassing traditionally popular genres such as comedy or adventure films. At the same time, modern audiences appear willing to immerse into more interactive and personalized viewing experiences. Documentaries, even in their linear version, involve high costs in all phases (pre-production, production, post-production) due to various inefficiencies, partly attributed to the lack of scientifically-proven cost-effective Information and Communications Technology (ICT) tools. To fill this gap, a set of innovative ICT tools is delivered that focus on supporting all stages of the documentary creation process, ranging from the documentary topic selection to its final delivery to the viewers. This paper provides an overview of the respective tools, elaborating on two specific tools that primarily focus on the interests and satisfaction of the targeted audience: the Integrated Trends Discovery tool and the Social Recommendation & Personalization tool, elaborating on their design, functionality and performance, and concludes with exposing the future plans and potential regarding these tools.
May 09, 2020 Next-generation ELISA diagnostic assay for Chagas Disease based on the combination of

Chagas Disease, caused by the protozoan Trypanosoma cruzi , is a major health and economic problem in Latin America for which no vaccine or appropriate drugs for large-scale public health interventions are yet available. Accurate diagnosis is essential for the early identification and follow up of vector-borne cases and to prevent transmission of the disease by way of blood transfusions and organ transplantation. Diagnosis is routinely performed using serological methods, some of which require the production of parasite lysates, parasite antigenic fractions Diagnostic tests are key tools to support disease surveillance, and to ulti-mately help stop transmission of the parasite. However currently available diagnostic methods have several limitations. Identification of novel biomarkers with improved diagnostic characteristics is a main priority. Recently, we conducted a large-scale screening looking for new T . cruzi antigens using short peptides displayed on a solid support at high-density. This led to the identification of several hundred novel antigenic epitopes. In this work we validated the serodiagnostic performance of 27 of these against an extended panel of human serum samples. Based on this analysis, we developed a proof-of-principle multiplex diagnostic kit by combining different validated reactive peptides. Overall, our data support the applicability of high-density peptide microarrays for the rapid identification and mapping epitopes that could be readily translated into novel and useful tools for diagnosis of Chagas disease.
Pregnancy Complications and Cardiovascular Disease Death 50-Year Follow-Up of the Child Health and Development Studies Epidemiology and Prevention

disease (CVD) death and how risk changes with age. This report presents a comprehensive investigation of the relation of the occurrence of multiple pregnancy complications to CVD death over 5 decades in a large pregnancy cohort. Methods and Results —We examined pregnancy events (1959–1967) and CVD death through 2011 in 14 062 women from the Child Health and Development Studies. CVD death was determined by linkage to California Vital Statistics and National Death Index. Women were a median age of 26 years at enrollment and 66 years in 2011. Preexisting hypertension (hazard ratio, 3.5; 95% confidence interval, 2.4–5.1); glycosuria (hazard ratio, 4.2; confidence interval, 1.3–13.1); late-onset preeclampsia (after week 34, hazard ratio, 2.0; confidence interval, 1.2–3.5); and hemoglobin decline over the second and third trimesters (hazard ratio, 1.7; confidence interval, 1.2–2.7) predicted CVD death. Delivery of a small-for-gestation or preterm infant and early-onset preeclampsia (by week 34) significantly predicted premature CVD death ( P <0.05 for age dependence). Preterm birth combined with hemorrhage, gestational hypertension, or preexisting hypertension identified women with a 4- to 7-fold increased risk of CVD death. Preeclampsia in combination with preexisting hypertension conferred a significant nearly 6-fold risk in comparison with a 4-fold risk for preexisting hypertension alone. Conclusions —We observed combinations of pregnancy complications that predict high risk of death and 2 new risk markers, glycosuria and hemoglobin decline. Obstetricians serve as primary care physicians for many young women and can readily use these complications to identify high-risk women to implement early prevention. ( Circulation . 2015;132:1234-1242. DOI: 10.1161/CIRCULATIONAHA.113.003901.) were combined to maximize power for investigating the risk of preeclampsia co-occurring with other pregnancy events. ‡Co-occurring events involving preeclampsia, and preterm and SGA delivery were tested for age dependence. Only the co-occurrence of gestational hypertension with preterm delivery and of preeclampsia with SGA were significantly age-dependent ( P =0.0119 and P =0.0018, respectively). The hazard ratios for these paired events are estimated at 60 years of age for co-occurring gestational hypertension with preterm delivery, and at 55 years of age for co-occurring preeclampsia with SGA delivery.
A 2.4-GHz 6.4-mW fractional-N inductorless RF synthesizer

A ring-oscillator-based cascaded synthesizer architecture incorporates a digital synchronous delay line and an analog noise trap to suppress the quantization noise of the ΣΔ modulator. Realized in 45-nm digital CMOS technology, the synthesizer exhibits an in-band phase noise of -109 dBc/Hz and an integrated jitter of 1.68 psrms.
OP-ICVA200158 1..7

OBJECTIVES: Vacuum bell and exercise therapy are non-invasive treatments for pectus excavatum (PE). The purpose of this study was to determine the effects of the physiotherapy programme applied in addition to vacuum bell treatment in patients with PE. METHODS: The study included 26 male patients with PE aged 11–18 years. Patients were randomly divided into 2 groups: group 1 received only vacuum bell treatment; group 2 had vacuum bell therapy and physiotherapy. Patient demographic and disease-related characteristics, type of sternal depression, perception of their deformity, postural evaluations, treatment satisfaction and quality of life were evaluated before and 12 weeks after treatment. RESULTS: From external chest circumference measurements related to PE, sternal depression and anthropometric index values showed improvement in both groups (P < 0.05), but better results were observed in group 2 than in group 1 (P < 0.05). Modified percent depth and scores from the T3 region (distance between the most prominent point of the sternum and the spinous process of the vertebra at the same level) showed improvement only in group 2 (P < 0.01), whereas severity of PE, the patient’s perception of his deformity and parental TH O R A C IC VC The Author(s) 2020. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. Interactive CardioVascular and Thoracic Surgery (2020) 1–7 ORIGINAL ARTICLE doi:10.1093/icvts/ivaa161 D ow naded rom http/academ ic.p.com /icvts/advance-art.1093/icvts/ivaa161/5910041 by gest on 24 Sptem er 2020 physiological quality-of-life scores improved in both groups (P < 0.05). Posture, satisfaction with treatment and the patients’ physiological quality-of-life scores were significantly better in group 2 (P < 0.05). CONCLUSIONS: Due to the additional improvements and greater satisfaction in the group receiving physiotherapy, we think that a proper rehabilitation programme should be applied simultaneously with the vacuum bell treatment in patients with PE. Clinical trial registration: clinicaltrials.gov : NCT04167865.
ЭЛЕКТРОПОРАЦИИ ДНК КОНСТРУКЦИЯМИ НА ДЕНДРИТНЫЕ

Today transfection of mammalian cell with DNA or RNA construction is the only method for delivering programmed information into the cell nucleus. Electroporation is most commonly used method of transfection in experiments with dendritic cell. The aim of electroporation is to permeabilize the membrane by passing electric impulse through the cell. Due to the increase permeability of the membrane chance DNA or RNA construction getting inside into the cell is increased, wherein survival of the cells is decreased. In the study male mice C57Bl/6 line 2-4 months old were used. From femur bones was isolated 20 × 10 6 bone marrow cells, which were cultured in 20 mL of complete RPMI-1640 for 7 days. To generate dendritic cells from BM cells, 100 ng/mL of rmFlt3-L was added to culture media at day 0. After 7 days of cultivation, the cell cultures were electroporated with control noncoding plasmids p5 (EP P5) or pmaxCCR9 encoding mouse chemokine receptor CCR9 (EP CCR9). The controls were cell cultures electroporated without any plasmids (mock EP) and cell cultures without electroporation (none EP). 5 × 10 5 cells were electroporated and resting for 10 minutes. After 10 minutes, cells were harvested and seeded into 24-well plates in 1 mL of culture medium and conditioning medium (1:1). Then, 50 ng/mL of Flt3-L was added to each well. The next day, transfected cells were collected and used for flow cytometry, qRT-PCR analysis. It was found that after electroporation in the groups mock EP, EP P5, EP CCR9 relative amount of live CD11c + dendritic cells was significantly less than in the non EP group. Moreover, in the EP P5 and EP CCR9 groups the frequency of live CD11c + dendritic cells was significantly less than in the mock EP group. Expression of the CD86 marker in the EP P5 and EP CCR9 groups was significantly higher than in the non EP and mock EP groups. Expression of the I-AB(MHCII) in the EP CCR9 group on cDC2s was significantly higher than in the non EP group. On plasmacytoid DCs (pDCs) and conventional type 2 DCs (cDC2s) in the EP CCR9 group expression of CCR9 was significantly higher than in the non EP group. Therefore, in this study, we demonstrated the effectiveness of electroporation, accompanied by the decrease in the survival rate and maturation of DCs.