Miracle-dz/UniFAR-FTUs huggingface dataset:
Normative Standards for HRpQCT Parameters in Chinese Men and Women

Assessing bone architecture using high‐resolution peripheral quantitative computed tomography (HRpQCT) has the potential to improve fracture risk assessment. The Normal Reference Study aimed to establish sex‐specific reference centile curves for HRpQCT parameters. This was an age‐stratified cross‐sectional study and 1072 ambulatory Chinese men (n = 544) and women (n = 528) aged 20 to 79 years, who were free from conditions and medications that could affect bone metabolism and had no history of fragility fracture. They were recruited from local communities of Hong Kong. Reference centile curves for each HRpQCT parameter were constructed using generalized additive models for location, scale, and shape with age as the only explanatory variable. Patterns of reference centile curves reflected age‐related changes of bone density, microarchitecture, and estimated bone strength. In both sexes, loss of cortical bone was only evident in mid‐adulthood, particularly in women with a more rapid fashion probably concurrent with the onset of menopause. In contrast, loss of trabecular bone was subtle or gradual or occurred at an earlier age. Expected values of HRpQCT parameters for a defined sex and age and a defined percentile or Z‐score were obtained from these curves. T‐scores were calculated using the population with the peak values as the reference and reflected age‐ or menopause‐related bone loss in an older individual or the room to reach the peak potential in a younger individual. These reference centile curves produced a standard describing a norm or desirable target that enables value clinical judgements. Percentiles, Z‐scores, and T‐scores would be helpful in detecting abnormalities in bone density and microarchitecture arising from various conditions and establishing entry criteria for clinical trials. They also hold the potential to refine the diagnosis of osteoporosis and assessment of fracture risk. © 2018 American Society for Bone and Mineral Research.
On Consistency of Compressive Spectral Clustering

Spectral clustering is one of the most popular methods for community detection in graphs. A key step in spectral clustering algorithms is the eigen decomposition of the $n\times n$ graph Laplacian matrix to extract its $k$ leading eigenvectors, where $k$ is the desired number of clusters among $n$ objects. This is prohibitively complex to implement for very large datasets. However, it has recently been shown that it is possible to bypass the eigen decomposition by computing an approximate spectral embedding through graph filtering of random signals. In this paper, we analyze the working of spectral clustering performed via graph filtering on the stochastic block model. Specifically, we characterize the effects of sparsity, dimensionality and filter approximation error on the consistency of the algorithm in recovering planted clusters.
Cell-Type-Specific Role of ΔFosB in Nucleus Accumbens In Modulating Intermale Aggression

A growing number of studies implicate the brain's reward circuitry in aggressive behavior. However, the cellular and molecular mechanisms within brain reward regions that modulate the intensity of aggression as well as motivation for it have been underexplored. Here, we investigate the cell-type-specific influence of ΔFosB, a transcription factor known to regulate a range of reward and motivated behaviors, acting in the nucleus accumbens (NAc), a key reward region, in male aggression in mice. We show that ΔFosB is specifically increased in dopamine D1 receptor (Drd1)-expressing medium spiny neurons (D1-MSNs) in NAc after repeated aggressive encounters. Viral-mediated induction of ΔFosB selectively in D1-MSNs of NAc intensifies aggressive behavior without affecting the preference for the aggression-paired context in a conditioned place preference (CPP) assay. In contrast, ΔFosB induction selectively in D2-MSNs reduces the time spent exploring the aggression-paired context during CPP without affecting the intensity of aggression per se. These data strongly support a dissociable cell-type-specific role for ΔFosB in the NAc in modulating aggression and aggression reward. SIGNIFICANCE STATEMENT Aggressive behavior is associated with several neuropsychiatric disorders and can be disruptive for affected individuals as well as their victims. Studies have shown a positive reinforcement mechanism underlying aggressive behavior that shares many common features with drug addiction. Here, we explore the cell-type-specific role of the addiction-associated transcription factor ΔFosB in the nucleus accumbens in aggression. We found that ΔFosB expression promotes aggressive behavior, effects that are dissociable from its effects on aggression reward. This finding is a significant first step in identifying therapeutic targets for the reduction of aggressive behavior across a range of neuropsychiatric illnesses.
Detection of illegal transfer of videos over the Internet

In this paper, a method for detecting infringements or modifications of a video in real-time is proposed. The method first segments a video stream into shots, after which it extracts some reference frames as keyframes. This process is performed employing a Singular Value Decomposition (SVD) technique developed in this work. Next, for each input video (represented by its keyframes), ordinal-based signature and SIFT (Scale Invariant Feature Transform) descriptors are generated. The ordinal-based method employs a two-level bitmap indexing scheme to construct the index for each video signature. The first level clusters all input keyframes into k clusters while the second level converts the ordinal-based signatures into bitmap vectors. On the other hand, the SIFT-based method directly uses the descriptors as the index. Given a suspect video (being streamed or transferred on the Internet), we generate the signature (ordinal and SIFT descriptors) then we compute similarity between its signature and those signatures in the database based on ordinal signature and SIFT descriptors separately. For similarity measure, besides the Euclidean distance, Boolean operators are also utilized during the matching process. We have tested our system by performing several experiments on 50 videos (each about 1/2 hour in duration) obtained from the TRECVID 2006 data set. For experiments set up, we refer to the conditions provided by TRECVID 2009 on "Content-based copy detection" task. In addition, we also refer to the requirements issued in the call for proposals by MPEG standard on the similar task. Initial result shows that our framework is effective and robust. As compared to our previous work, on top of the achievement we obtained by reducing the storage space and time taken in the ordinal based method, by introducing the SIFT features, we could achieve an overall accuracy in F1 measure of about 96% (improved about 8%).
Fixed-dose combination antihypertensive medications, adherence, and clinical outcomes: A population-based retrospective cohort study

Background The majority of people with hypertension require more than one medication to achieve blood pressure control. Many patients are prescribed multipill antihypertensive regimens rather than single-pill fixed-dose combination (FDC) treatment. Although FDC use may improve medication adherence, the impact on patient outcomes is unclear. We compared clinical outcomes and medication adherence with FDC therapy versus multipill combination therapy in a real-world setting using linked clinical and administrative databases. Methods and findings We conducted a population-based retrospective cohort study of 13,350 individuals 66 years and older in Ontario, Canada with up to 5 years of follow-up. We included individuals who were newly initiated on one angiotensin-converting enzyme inhibitor (ACEI) or angiotensin II-receptor blocker (ARB) plus one thiazide diuretic. High-dimensional propensity score matching was used to compare individuals receiving FDC versus multipill therapy. The primary outcome was a composite of death or hospitalization for acute myocardial infarction (AMI), heart failure, or stroke. We conducted 2 analyses to examine the association between adherence and patient outcomes. First, we performed an on-treatment analysis to determine whether outcomes differed between groups while patients were on treatment, censoring patients when they first discontinued treatment, defined as not receiving medications within 150% of the previous days’ supply. Second, we conducted an intention-to-treat analysis that followed individuals allowing for breaks in treatment to quantify the difference in drug adherence between groups and assess its impact on clinical outcomes. As expected, there was no significant difference in the primary outcome between groups in the on-treatment analysis (HR 1.06, 95% CI 0.86–1.31, P = 0.60). In the intention-to-treat analysis, the proportion of total follow-up days covered with medications was significantly greater in the FDC group (70%; IQR 19–98) than in the multipill group (42%, IQR 11–91, P < 0.01), and the primary outcome was less frequent in FDC recipients (3.4 versus 3.9 events per 100 person-years; HR 0.89, 95% CI 0.81–0.97, P < 0.01). The main limitations of this study were the lack of data regarding cause of death and blood pressure measurements and the possibility of residual confounding. Conclusions Among older adults initiating combination antihypertensive treatment, FDC therapy was associated with a significantly lower risk of composite clinical outcomes, which may be related to better medication adherence.
Neurovascular stent artifacts in 3D‐TOF and 3D‐PCMRI: Influence of stent design on flow measurement

The morphological and hemodynamic evaluations of neurovascular diseases treated with stents would benefit from noninvasive imaging techniques such as 3D time‐of‐flight MRI (3D‐TOF) and 3D phase contrast MRI (3D‐PCMRI). For this purpose, a comprehensive evaluation of the stent artifacts and their impact on the flow measurement is critical.
Relationship between body weight perception and weight-related behaviours

Objectives This study aimed to examine the prevalence of weight misperception and to explore the association of weight perception with physical activity and dietary intake. Methods A population-based, nationally representative cross-sectional survey was conducted of 920 Thai adolescents aged 13 to 24 years. Respondents were selected using stratified multistage sampling. Respondents who agreed to participate were asked to complete the Youth Risk Behavior Questionnaire. Results Females were more likely than males to misperceive themselves as overweight (21.8% vs. 11.7%), whereas males were more likely than females to misperceive themselves as underweight (12.3% vs. 3.4%). Males were more likely than females to report a high intake of vegetables (45.7% vs. 38.0%), milk (39.1% vs. 38.0%), 100% fruit juice (20.9% vs. 17.7%) and soda or pop (38.5% vs. 20.6%). Males were also more likely than females to report vigorous physical activity of more than 60 minutes per day (38.1% vs. 21.3%) and vigorous exercise to strengthen or tone muscles (37.3% vs. 13.2%). Conclusions Gender differences in dietary intake and physical activity were identified. However, there were no significant differences in dietary intake and physical activity between adolescents who correctly perceived themselves as overweight and those who misperceived themselves as overweight.
Light‐controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B

Transcriptional regulation is a useful strategy for gene therapy and for biomedical research. Unlike chemically regulated transcriptional approaches, spatiotemporal control of transcription using optogenetic tools is a powerful technology for the analysis of single cells. For light to penetrate into tissues, it is desired to use photoreceptors absorbing red/far‐red light with a low‐molecular mass applicable for the use of virus vectors, and a photoswitch using the photoreceptor needs to be constructed as a single expression vector. Herein, we describe an optogenetic tool based on Arabidopsis thaliana phytochrome (Phy) B and its binding partner, phytochrome‐interacting factor (PIF) 6. We generated a truncated PhyB, which allowed for reversible association with PIF6 by red/far‐red light illumination. The red light illumination only for 5 min induced PhyB translocation from the cytoplasm into the nucleus by the association with PIF6, resulting in transcriptional activation based on Gal4 DNA‐binding domain and the upstream activating sequence of Gal system. The nucleocytoplasmic shuttling vector using PhyB and PIF6 might be applicable for transcriptional regulation in tissue experiments.
Neural overlap in item representations across episodes impairs context memory

We frequently encounter the same item in different contexts, and when that happens, memories of earlier encounters can get reactivated in the brain. Here we examined how these existing memories are changed as a result of such reactivation. We hypothesized that when an item’s initial and subsequent neural representations overlap, this allows the initial item to become associated with novel contextual information, interfering with later retrieval of the initial context. That is, we predicted a negative relationship between representational similarity across repeated experiences of an item and subsequent source memory for the initial context. We tested this hypothesis in an fMRI study, in which objects were presented multiple times during different tasks. We measured the similarity of the neural patterns in lateral occipital cortex that were elicited by the first and second presentations of objects, and related this neural overlap score to source memory in a subsequent test. Consistent with our hypothesis, greater item-specific pattern similarity was linked to worse source memory for the initial task. Our findings suggest that the influence of novel experiences on an existing context memory depends on how reliably a shared component (i.e., same item) is represented across these episodes.
LIFE: A FLEXIBLE TESTBED FOR LIGHT FIELD EVALUATION

Recording and imaging the 3D world has led to the use of light fields. Capturing, distributing and presenting light field data is challenging, and requires an evaluation platform. We define a framework for real-time processing, and present the design and implementation of a light field evaluation system. In order to serve as a testbed, the system is designed to be flexible, scalable, and able to model various end-to-end light field systems. This flexibility is achieved by encapsulating processes and devices in discrete framework systems. The modular capture system supports multiple camera types, general-purpose data processing, and streaming to network interfaces. The cloud system allows for parallel transcoding and distribution of streams. The presentation system encapsulates rendering and display specifics. The real-time ability was tested in a latency measurement; the capture and presentation systems process and stream frames within a 40 ms limit.
Expression of TIGIT and FCRL3 is Altered in T Cells from Patients with Distinct Patterns of Chronic Autoimmune Thyroiditis

Abstract Background Co-inhibitory receptors (IR), such as TIGIT and FCRL3, provide a checkpoint against highly destructive immune responses. Co-expression of TIGIT and FCRL3, in particular, has been linked to the HELIOS+ subset of regulatory CD4+FOXP3+T-cells. Of these, CD4+FOXP3-exon(E)2+ cells have higher expression of IR and exhibit strongest suppressive properties. Nevertheless, how the expression of TIGIT, FCRL3, HELIOS, and FOXP3E2 is regulated in chronic autoimmune thyroiditis (AT), is not known. Methods Thirty patients with AT [encompassing spontaneously euthyroid (euAT), hypothyroid-untreated and L-thyroxine-treated cases)] and 10 healthy controls (HC) were recruited. FCRL3, TIGIT, HELIOS and FOXP3E2 mRNA expression levels in peripheral blood (PB) T cells were measured via quantitative real-time PCR and compared to clinicopathological factors. Results The TIGIT and FCRL3 expression levels from T cells of AT cases were inversely related to the thyroid volume, and were significantly increased in hypothyroid patients (on+off L-thyroxine), but not euAT cases. The FCRL3 expression in PB T cells positively correlated with thyroid-peroxidase autoantibody levels; by contrast, T cells from aged AT patients and combined samples (AT+HC) accumulated more TIGIT mRNA. The patients with higher TIGIT mRNA levels had a greater prevalence of hypothyroidism, showing higher peak thyrotropin levels at diagnosis or at follow-up. Conclusions Multiple IR, namely FCRL3 and TIGIT, but not the transcription factors HELIOS and FOXP3E2, showed increased mRNA levels in PB T cells from end-stage, long-standing and/or more aggressive AT, in proportion to disease severity. A relation with major clinical subphenotypes was observed, thereby identifying IR as potentially important players in AT.
Winning Awards or Winning Citations: A Retrospective Look at the Consistency between Evaluative Metrics

Appropriate evaluation of information systems research papers ensures that our institutions and review processes stay viable. In the short run, we typically assess research value through research awards, while, in the longer term, we typically assess research value based on how the research community sees and draws from particular published research papers. In this study, we examine the consistency between two metrics for assessing research value: research awards and citations. To do so, we focus on a premier journal, MIS Quarterly. We found that rarely are the “papers of the year” the ones cited the most. We offer possible explanations for this discrepancy based on assessing papers’ originality and utility and their citation patterns.
Basics of Antibody Phage Display Technology

Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments. Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.
OXYGEN AND HAEMOGLOBIN PAIR MODEL FOR SICKLE CELL ANEAMIA PATIENTS

In this paper, we studied the concentration of oxygen in the haemoglobin of the sickle cell patient using the Oxygen- heamoglobin pair (OHP) model which is an impulsive Hill-Fokker-Planck equation . Using the B-transform of Oyelami and Ale we determine the best concentration for oxygen or haemoglobin to support the patient using life-supporting drug like nitric oxide providing drugs. Since the sickle nature of the erythrocyte of the patient has the contributory factor to sickling problem and there is the need to correct this defect and to enhance the haemoglobin affinity for oxygen absorption, thereby, reducing the patient‟s physiological problem. Using Lagrangian optimization method coupled with the application of simple calculus and B
Developing a Cas9‐based tool to engineer native plasmids in Synechocystis sp. PCC 6803

The oxygenic photosynthetic bacterium Synechocystis sp. PCC 6803 (S6803) is a model cyanobacterium widely used for fundamental research and biotechnology applications. Due to its polyploidy, existing methods for genome engineering of S6803 require multiple rounds of selection to modify all genome copies, which is time‐consuming and inefficient. In this study, we engineered the Cas9 tool for one‐step, segregation‐free genome engineering. We further used our Cas9 tool to delete three of seven S6803 native plasmids. Our results show that all three small‐size native plasmids, but not the large‐size native plasmids, can be deleted with this tool. To further facilitate heterologous gene expression in S6803, a shuttle vector based on the native plasmid pCC5.2 was created. The shuttle vector can be introduced into Cas9‐containing S6803 in one step without requiring segregation and can be stably maintained without antibiotic pressure for at least 30 days. Moreover, genes encoded on the shuttle vector remain functional after 30 days of continuous cultivation without selective pressure. Thus, this study provides a set of new tools for rapid modification of the S6803 genome and for stable expression of heterologous genes, potentially facilitating both fundamental research and biotechnology applications using S6803.
LTBSG1, a New Allele of BRD2, Regulates Panicle and Grain Development in Rice by Brassinosteroid Biosynthetic Pathway

Panicle architecture and grain size are two important agronomic traits which determine grain yield directly in rice. In the present study, a mutant named ltbsg1 (longer top branch and shorter grain 1) was isolated from the cultivar “Zhenong 34” (Oryza sativa L. ssp. indica) by ethyl methane sulfonate (EMS) mutagenesis. The target gene was studied through phenotype observation, genetic analysis, map-based cloning and functional analysis. The histocytological analysis indicated that the elongated top branch and shorter grain of mutant ltbsg1 were caused from the defects of cell elongation. The ltbsg1 gene in mutant revealed a single nucleotide substitution (G-A) in the exon 2 of LOC_Os10g25780, causing an amino acid variation (Glycine-Arginine) in the FAD (Flavin-adenine dinucleotide)-binding domain of delta (24)-sterol reductase, which was involved in the brassinosteroid (BR) biosynthesis. LTBSG1 was constitutively expressed and the protein was widely localized in chloroplast, nucleus and cytomembrane. The ltbsg1 seedlings had a lower endogenous BR level and could be restored to the phenotype of wild type by exogenous BR. The LTBSG1 knock-out lines showed similar phenotype defects as mutant ltbsg1, which confirmed that LTBSG1 was responsible for top branch elongation and grain size reduction. Furthermore, LTBSG1 along with other BR-related genes were feedback-regulated due to their obvious altered expression in mutant ltbsg1. This study demonstrated that LTBSG1 could play a new role in regulating panicle and grain development by BR biosynthetic pathway.