Miracle-dz/UniFAR-FTUs huggingface dataset:
The national school lunch and competitive food offerings and purchasing behaviors of high school students.

BACKGROUND Across the nation, schools have become actively involved in developing obesity prevention strategies both in classrooms and in cafeterias. We sought to determine the type of foods being offered during lunch in the cafeteria of 3 public high schools in 1 county and if this reflects the purchasing patterns of students. By labeling foods based on nutrient density using a stoplight approach of green, yellow, and red colors, we were able to categorize all foods including the National School Lunch Program (NSLP) and competitive foods available in the cafeteria. METHODS Over a 4-week cycle, daily food purchases were gathered and the proportions of green, yellow, and red foods offered and purchased was compared. RESULTS Findings from this study suggest that students in these 3 high schools purchased foods in relative proportion to what was available in the school cafeteria for the NSLP. Green and yellow foods included in the NSLP comprised 77% of the offerings and 73% of the purchases. In contrast, 61% of the competitive foods were classified as red foods, and the purchasing of red foods made up 83% of competitive food sales. These results indicate that students purchase foods of minimal nutritional value at greater proportions in the school cafeteria. CONCLUSIONS These results suggest that the nutritional policy for the NSLP promotes the offerings of a wide array of foods. Schools should consider a nutrition policy that regulates the sale of competitive foods.
Inhibition of leukemia cell proliferation by receptor-mediated uptake of c-myb antisense oligodeoxynucleotides.

Exposure of human leukemia HL-60 cells to an oligodeoxynucleotide complementary to an 18-base sequence (codons 2-7) of c-myb-encoded mRNA has previously been shown to result in inhibition of cell proliferation. Because HL-60 cells express high levels of transferrin receptor we adapted a DNA delivery system based on receptor-mediated endocytosis to introduce myb oligomers complexed with a transferrin-polylysine conjugate into those cells. A DNA.RNA duplex resistant to S1 nuclease digestion was detected as early as 12 hr after culture of HL-60 cells in the presence of the myb antisense/transferrin-polylysine complex. Exposure of HL-60 cells to the myb antisense/transferrin-polylysine complex resulted in rapid and profound inhibition of proliferation and loss of cell viability much more pronounced than that occurring in cells exposed to free myb antisense oligodeoxynucleotides. The transferrin-polylysine/myb sense complex or the transferrin-polylysine conjugate alone had no effect on HL-60 cell proliferation and viability. These findings indicate that myb synthetic oligodeoxynucleotides enter efficiently into HL-60 by transferrin receptor-mediated endocytosis and exert a profound biological effect. Such a delivery system could exploit other ligand-receptor interactions for the selective delivery of oncogene-targeted antisense oligodeoxynucleotides.
Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production

Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened.
Pain-related Insomnia Versus Primary Insomnia: A Comparison Study of Sleep Pattern, Psychological Characteristics, and Cognitive-behavioral Processes

Background:Recent applications of cognitive-behavior therapy for primary insomnia in the management of pain-related insomnia are based on the implicit assumption that the 2 types of insomnia share the same presentation and maintaining mechanisms. The objectives of this study were to compare the characteristics of patients who have pain-related insomnia with those reporting primary insomnia and to identify psychological factors that predict pain-related insomnia. Methods:Chronic pain patients with concomitant insomnia (n=137; Pain-related Insomnia Group) completed a selection of questionnaires that measure sleep patterns, psychological attributes, and cognitive-behavioral processes associated with the persistence of insomnia. Their responses were compared with those of primary insomnia patients (n=33; Primary Insomnia Group), using 3 sets of multivariate analyses of covariance that took account of demographic differences. Hierarchical regression analyses were performed to identify predictors of insomnia severity among the chronic pain patients. Results:The Pain-related Insomnia Group did not differ from the Primary Insomnia Group in their pattern and severity of sleep disturbance. The 2 groups were largely comparable in terms of their psychological characteristics, except that the Primary Insomnia Group was distinguishable from the Pain-related Insomnia Group by their greater tendency to worry. Patients in the Pain-related Insomnia Group reported levels of sleep-related anxiety and presleep somatic arousal that matched with those reported by patients in the Primary Insomnia Group. However, relative to patients in the Pain-related Insomnia Group, those in the Primary Insomnia Group reported more dysfunctional sleep beliefs and presleep cognitive arousal. In addition to pain intensity, depression, and presleep cognitive arousal were significant predictors of insomnia severity within the Pain-related Insomnia Group. Conclusions:There are more similarities than differences between the 2 types of insomnia. Besides pain, mood, and presleep, thought processes also seem to have a role in the manifestation of pain-related insomnia. It is suggested that hybrid treatments that seek to simultaneously address factors across these domains may represent more effective treatments than 1-dimensional interventions.
Theca cell cytochrome P450 17-hydroxylase and aromatase messenger ribonucleic acid abundance and serum steroid levels during follicular atresia associated with incubation behavior in the domestic turkey hen.

This study was designed to examine changes in cytochrome P450 17 alpha-hydroxylase (C17) and aromatase (ARO) mRNA contents in the theca layer of preovulatory follicles (POF) as turkey hens transit from egg laying to incubation. Hens were grouped into the following categories: 1) laying hens--laid one egg per day and nested 1-2 times per day; 2) transitional hens--laid one egg per day and nested > 4 times per day; and 3) Day 1, Day 3, and Day 5 incubating hens--laid no eggs for 2, 4, or 6 days, respectively, and nested > 4 times per day. Small white follicles (SWF) and the theca layer from the largest (F1) and the third (F3), fifth (F5), and seventh (F7) largest POF were dispersed and challenged with testosterone (T) for 5 h. Relative levels of C17 and ARO mRNA were examined from the theca layers of F1, F3, F5, F7, and SWF. The number of atretic follicles increased from 0 (layers) to 8 (Day 5 incubating hens). Serum LH, progesterone (P), and estradiol (E), but not T, declined on Day 1 of incubation. Basal levels of P, T, and E from theca and SWF cells declined in incubating hens. Both basal and T-stimulated theca and SWF production of E decreased in incubating hens. C17 and ARO mRNA declined in SWF, F7, and F5 during follicular atresia. It is suggested that reduced gene expression of ovarian steroidogenic enzymes may be a partial determinant of reduced circulating sex steroid levels in incubating hens. (ABSTRACT TRUNCATED AT 250 WORDS)
Proteomic Characterization of Postmortem Amyloid Plaques Isolated by Laser Capture Microdissection*[boxs]

The presence of amyloid plaques in the brain is one of the pathological hallmarks of Alzheimer's disease (AD). We report here a comprehensive proteomic analysis of senile plaques from postmortem AD brain tissues. Senile plaques labeled with thioflavin-S were procured by laser capture microdissection, and their protein components were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified a total of 488 proteins coisolated with the plaques, and we found multiple phosphorylation sites on the neurofilament intermediate chain, implicating the complexity and diversity of cellular processes involved in the plaque formation. More significantly, we identified 26 proteins enriched in the plaques of two AD cases by quantitative comparison with surrounding non-plaque tissues. The localization of several proteins in the plaques was further confirmed by the approach of immunohistochemistry. In addition to previously identified plaque constituents, we discovered novel association of dynein heavy chain with the plaques in human postmortem brain and in a double transgenic AD mouse model, suggesting that neuronal transport may play a role in neuritic degeneration. Overall, our results revealed for the first time the sub-proteome of amyloid plaques that is important for further studies on disease biomarker identification and molecular mechanisms of AD pathogenesis.
Effects of various preprocessing techniques to Turkish text categorization using n-gram features

Natural Language Processing (NLP) is a prominent subject which includes various subcategories such as text classification, error correction, machine translation, etc. Unlike other languages, there are limited number of Turkish NLP studies in literature. In this study, we apply text classification on Turkish documents by using n-gram features. Our algorithm applies different preprocessing techniques, namely, n-gram choice (character level or word level, bigram or trigram models), stemming, and use of punctuation, and then determines the Turkish document's author and genre, and the gender of the author. For this purpose, Naive Bayes, Support Vector Machines and Random Forest are used as classification techniques. Finally, we discuss the effects of above mentioned preprocessing techniques to the performance of Turkish text classification.
Comparative analysis of global consumer behaviour in the context of different manual dishwashing methods

This laboratory study presents an overview of the global manual dishwashing behaviour. The focus of the investigation was to analyse individual attitudes towards manual dishwashing and to determine the amount of water and energy used, as well as the cleaning performance achieved. Additionally, manual dishwashing was compared with automatic dishwashers. Two hundred eighty-nine participants from 29 countries took part in this investigation. Each consumer had to wash up a complete soiled dishwasher load consisting of 12 place settings based on both international and local performance test standards for automatic dishwashers. Country-specific aspects such as tableware, food residues or washing up equipment were considered. In order to analyse individual consumer's behaviour, each participant was recorded on video and had to fill out a questionnaire. The resource consumption for washing up dishes was measured during the tests. At the end of each trial, the cleaning result of the washed up tableware was assessed. To compare manual with automatic dishwashing, country-specific dishwasher models were tested in parallel with three different programmes with the same soiled dish samples. The study provides comprehensive data about the average resource consumption for manual dishwashing for a specific load for each country. The average water consumption per country reached 34.7 l up to 160.1 l, and individual values ranged from 18.3 l to 472.8 l. The lowest used average heat quantity accounted for 0.9 kWh per country, while the highest amount was five times higher. The cleaning results did not differ much between the countries: the average test results were between 2.2 and 2.8 on a scale between 0 and 5. The automatic dishwasher tests showed differences between both the machines and the programmes. All machines achieved lower water consumption values than the average consumers with about 9.6 l to 26.7 l of water on average. The energy consumption ranged from 0.5 kWh on average up to 2.0 kWh. The cleaning results of the dishwasher tests varied highly ranging from 1.1 in a quick programme to 4.4 in an intensive programme. The study comes to the conclusion that automatic dishwashing is more superior as compared with manual dishwashing in terms of performance and resource consumption under the tested conditions. Furthermore, it points out that washing up dishes under running tap water is the most water-consuming manual dishwashing method of all investigated ones. A high lack of knowledge about the benefits of automatic dishwashing compared with manual dishwashing can still be identified among consumers.
Cardiac tumorigenic potential of induced pluripotent stem cells in an immunocompetent host with myocardial infarction.

AIM Genetic reprogramming of somatic cells with stemness genes to restore their pluripotent status is being studied extensively to generate pluripotent stem cells as an alternative to embryonic stem cells. This study was designed to examine the effectiveness of skeletal myoblast-derived induced pluripotent stem cells (SkiPS) from young male Oct4/GFP transgenic mice for regeneration of the infarcted heart. METHODS & RESULTS A mouse model of permanent coronary artery ligation was developed in young female immunocompetent C57BL/6J or C57BL/6x129S4 SV/jae Oct4/GFP mice. SkiPS labeled with Q-dots (3 × 10(5) in 10 µl basal Dulbecco's modiﬁed Eagle's medium) were transplanted in and around the area of infarct immediately after coronary artery ligation (n = 16) under direct vision. Control mice (n = 12) were injected with the same number of skeletal myoblasts. Histological studies documented successful engraftment of SkiPS in all the surviving animals 4 weeks later. However, six of the 16 SkiPS-transplanted (37.5%) animal hearts showed intramural teratomas, whereas no tumor growth was observed in the control mice. Q-dot-labeled donor cells were also observed at the site of tumors. Histological studies revealed that teratomas were composed of cells from all of the three embryonic germ layers. Ultra-structure studies confirmed the histological findings and showed regions with well-organized myofibrillar structures in the tumors. CONCLUSION Undifferentiated induced pluripotent stem cells should not be recommended for cardiac transplantation unless screened for specific teratogenic precursors or predifferentiated into cardiac lineage prior to transplantation.
Efficient hardware implementation of threshold neural networks

Area and Noise to Signal Ratio (NSR) are two main factors in hardware implementation of neural networks. Despite attempts to reduce the area of sigmoid and hyperbolic tangent activation functions, they cannot achieve the efficiency of threshold activation function. A new NSR efficient architecture for threshold networks is proposed in this paper. The proposed architecture uses different number of bits for weight storage in different layers. The optimum number of bits for each layer is found based on the mathematical derivation using stochastic model. Network training is done using the recently introduced learning algorithm called Extreme Learning Machine (ELM). A 4-7-4 network is considered as a case study and its hardware implementation for different weight accuracies is investigated. The proposed design is more efficient considering area × NSR as a performance metric. VLSI implementation of the proposed architecture using a 0.18 μm CMOS process is presented which shows 44.16%, 58.04 % and 67.30% improvement for total number of bits equal to 16, 20 and 24.
Different hepatitis b virus genotypes are associated with different mutations in the core promoter and precore regions during hepatitis B e antigen seroconversion

Mutations in the core promoter and precore regions are frequently found in hepatitis B e antigen (HBeAg)‐negative patients, but precore stop codon mutation is restricted to hepatitis B virus (HBV) genotypes that have T at nucleotide 1858. The aims of this study were to determine the role of core promoter and/or precore mutations in HBeAg seroconversion and their impact on the subsequent course of liver disease, and to determine if core promoter mutations are more frequently selected in patients with HBV genotypes that preclude the development of precore stop codon mutation. Serial sera from 45 patients with chronic HBV infection were polymerase chain reaction (PCR)‐amplified, and the HBV core promoter and precore regions were sequenced. Ninety‐two percent of patients had core promoter or precore mutations after HBeAg seroconversion: 42% had core promoter changes only, 38% had precore stop codon mutations only, and 12% had changes in both regions. Seventy‐three percent of the patients had persistently normal aminotransferases, and only 8% had multiple flares in aminotransferases after HBeAg seroconversion. Core promoter changes were significantly more common in patients infected with HBV who have C at nucleotide 1858 (91% vs. 27%; P < .01), while precore stop codon changes were exclusively found in patients infected with HBV who have T at nucleotide 1858 (87% vs. 0; P < .01). The vast majority of our patients had core promoter and/or precore mutations after HBeAg seroconversion. Nevertheless, most patients had sustained remission of liver disease. Our data suggest that core promoter changes are preferentially selected in patients infected with HBV genotypes that preclude the development of precore stop codon mutation.
Lorentz violation and ultrahigh-energy photons

The propagation of photons, electrons and positrons at ultrahigh energies above $\ensuremath{\sim}{10}^{19}\text{ }\text{ }\mathrm{eV}$ can be changed considerably if the dispersion relations of these particles are modified by terms suppressed by powers of the Planck scale. We recently pointed out that the current nonobservation of photons in the ultrahigh-energy cosmic ray flux at such energies can put strong constraints on such modified dispersion relations. In the present work we generalize these constraints to all three Lorentz invariance-breaking parameters that can occur in the dispersion relations for photons, electrons and positrons at first- and second-order suppression with the Planck scale. We also show how the excluded regions in these three-dimensional parameter ranges would be extended if ultrahigh-energy photons were detected in the future.
LABAQM - A SYSTEM FOR QUALITATIVE MODELLING AND ANALYSIS OF ANIMAL BEHAVIOUR

Tracking of a laboratory animal and its behaviour interpretation based on frame sequence analysis have been traditionally quantitative and typically generates large amounts of temporally evolving data. In our work we are dealing with higher-level approaches such as conceptual clustering and qualitative modelling in order to represent data obtained by tracking. We present the LABAQM system developed for the analysis of laboratory animal behaviours. It is based on qualitative modelling of animal motions. We are dealing with the cognitive phase of the laboratory animal behaviour analysis as a part of the pharmacological experiments. The system is based on the quantitative data from the tracking application and incomplete domain background knowledge. The LABAQM system operates in two main phases: behaviour learning and behaviour analysis. The behaviour learning and behaviour analysis phase are based on symbol sequences, obtained by the transformation of the quantitative data. Behaviour learning phase includes supervised learning procedure, unsupervised learning procedure and their combination. The fusion of supervised and unsupervised learning procedures produces more robust models of characteristic behaviours, which are used in the behaviour analysis phase.
Infusions of Diazepam and Allopregnanolone into the Midbrain Central Gray Facilitate Open-Field Behavior and Sexual Receptivity in Female Rats

We have previously shown that blocking GABAA receptors with bicuculline in the dorsal and lateral quadrants of the rostral two-thirds of the midbrain central gray (MCG) in rats reduces the lordosis quotient (LQ) and proceptive behaviors without increasing the defensive index (McCarthy, Pfaff, and Schwartz-Giblin, 1991b), indicating that tonic release of endogenous GABA in the MCG has a primary facilitatory effect on reproductive behaviors. The present study tests whether two modulators of the GABAA-benzodiazepine-receptor complex, diazepam and the progesterone metabolite, allopregnanolone, facilitate female receptivity as well as open-field behavior. Ovariectomized estradiol benzoate (EB)-treated rats were bilaterally infused in the MCG with vehicle, diazepam, or allopregnanolone and tested 20 min after diazepam and 12 min after allopregnanolone. Animals were tested once each for both receptive or open-field behavior with testing sessions being at least 1 week apart in a crossover design between vehicle and one of the two drugs. Both diazepam and allopregnanolone significantly increased the lordosis quotient in a dose-dependent manner, the lowest effective dose tested being 100 and 250 ng, respectively. In the open-field test, infusion of diazepam (100 ng) in EB-treated rats significantly increased the ratio of central squares entered to total squares entered (C/T) without a change in total activity. Rats without EB elicited comparable C/T ratios when tested with five times as much diazepam. Diazepam infusions at more dorsal sites in the midbrain and in overlying cortex were without effect on C/T ratios in EB-treated rats. Allopregnanolone (500 ng) infusions in MCG of EB-treated rats did not alter C/T ratios compared to animals without EB. Our results are consistent with diazepam and allopregnanolone acting through the GABAA receptors of the MCG to enhance female sexual receptivity and with estradiol potentiating the effect of diazepam in the MCG to increase open-field behavior.