Effects Of Different Doses Of Probiotic (Biomin)® On Some Hematological And Biochemical Parameters In Chicken

This study was carried out to evaluate effects of probiotic (BIOMIN) ® at different doses (0.15, 0.3, 1.5, 7.5, 15 g/kg diet) on some hematological and biochemical parameters in chickens. The number of broiler chickens are 48 at one day old Ross kind were used . Results show that body weight increased significantly (p≤0.05) as probiotic dose, while both Hb concentration and PCV% are not changed in relation to probiotic doses. Reduction of serum cholesterol level was clear and dependent on the contrary changes in both serum protein and urea, which inceased with Effects Of Different Doses Of Probiotic (Biomin)® On Some Hematological . 46 dose elevation, on the other hand, serum glucose level changes unclearly in different dose groups. Finally results show very high dose (15 g/kg diet) of probiotic (BIOMIN) ® lead to death of all chickens in this group at the 3 rd day of experiment. Introduction Probiotic in Greek means "for life" (1) and can be defined as a live microbial feed supplementations, which beneficially affects the host animal by improving its intestinal balance (2), or a monoor defined mixed culture of live microorganism which, applied to animal or man, beneficially affected the host by improving the properties of indigenous gastrointestinal bacteriota (3), but restricted to products that these properties are: contain live microorganism (e.g.: freeze-dried cells or in fresh or fermented products), improve the health and wellbeing of animals or man (including growth promotion), can have their effect on all host mucosal surface including: the mouth and gastrointestinal tract, the upper respiratory tract, and the urogenital tract (4). However, its important to distinct more details about probiotc action, that can put in the following categories: First, production of inhibitory substances, which include: Hydrogen peroxide (H2O2) that protect host cell from pathogenic microorganisms by oxidation-reduction reactions (5), Antibacterial substances as Bacteriocin (protein produced by certain bacteria and act as antibacterial agent) (4), and Nicin and Reuterin (broad spectrum antibacterial agent in poultry produced by different types of bacteria) (6). The second category is adhesion sites blocking, already microorganisms adhere on gastrointestinal mucosa by cell wall polysaccharide, probiotic prevent this adhesion by different mechanisms as: carbohydrate-binding specificities, preventing of microorganism to get to Mannan receptors, or lactobacillus competitive adhesion to epithelial cells of gastrointestinal mucosa blocking pathogenic bacteria touching these cells (4). The third category is competition for essential nutrients, for e.g.: abnormal balance of bacteria in the gastrointestinal tract is capable of utilizing all of the potential carbon sources in the environment (7). Also the fourth category is toxin receptor destruction, yeast containing probiotics act by this mechanism more than bacteria containing probiotics (8), briefly this action do by toxin binding to yeast cell receptor, so protecting epithelial cells from toxins (9). In addition also chick immunostimulation by bacteria containing probiotics is one of the most important mechanism to utilize the normal equilibrium of microflora in the gut (10). From our survey on the past literatures we noted that most studies are directed away from probiotic effects on the body biochemical parameters, therefore, the present study was undertaken to study the