Miracle-dz/UniFAR-FTUs huggingface dataset:
Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions

Triatoma rubrofasciata (T. rubrofasciata), one kind of triatomine insects, is the vector of Trypanosoma cruzi (T. cruzi), which lead to American trypanosomiasis. Although the gut microbiome may play an essential role in the development and susceptibility of triatomine, there is limited research on the gut microbiota of T. rubrofasciata. To elucidate the effect of the vector’s developmental stages and environmental conditions on the gut microbiome, we employed 16S rRNA gene sequencing to profile the gut bacterial community diversity and composition of T. rubrofasciata. Significant shifts were observed in the overall gut microbe diversity and composition across the development of T. rubrofasciata and specific bacteria were detected in different stages. Serratia and Burkholderia-Caballeronia-Paraburkholderia were dominant in the 1st nymphal stage, while the abundance of Staphylococcus was low in the 1st nymphal stage. Oceanicaulis were undetectable in the adult stage and Odoribacter peaked in the 2nd nymphal stage. Moreover, Staphylococcus was correlated negatively with Serratia. Likewise, the total gut microbiota diversity and composition of T. rubrofasciata differentiated significantly by environmental conditions. The ingestion of a bloodmeal increased alpha diversity of gut bacterial communities, and Staphylococcus was more abundant in laboratory-reared bugs whereas Enterococcus enriched in wild-caught bugs. Furthermore, Pantoea was negatively correlated with Staphylococcus, and positively related to Bacillus only. The phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) algorithm showed obvious metagenomic functional differences by environmental conditions, and Chagas disease relevant pathway was enriched in wild-caught T. rubrofasciata.
The role of structural pleiotropy and regulatory evolution in the retention of heteromers of paralogs

Gene duplication is a driver of the evolution of new functions. The duplication of genes encoding homomeric proteins leads to the formation of homomers and heteromers of paralogs, creating new complexes after a single duplication event. The loss of these heteromers may be required for the two paralogs to evolve independent functions. Using yeast as a model, we find that heteromerization is frequent among duplicated homomers and correlates with functional similarity between paralogs. Using in silico evolution, we show that for homomers and heteromers sharing binding interfaces, mutations in one paralog can have structural pleiotropic effects on both interactions, resulting in highly correlated responses of the complexes to selection. Therefore, heteromerization could be preserved indirectly due to selection for the maintenance of homomers, thus slowing down functional divergence between paralogs. We suggest that paralogs can overcome the obstacle of structural pleiotropy by regulatory evolution at the transcriptional and post-translational levels.
Attention Bias and Recognition of Sexual Images

Attention to sexual stimuli is necessary for the development of sexual response, yet while there is some evidence of attention bias in favor of sexual stimuli, the direction and magnitude of the effect remain unknown. A high-powered sample of 113 participants was tested using the dot-probe task (DPT) and picture recognition task (PRT) to measure visuospatial attention to erotic images. Participants showed no attention bias in the DPT (rB = 0.201, p = 0.064) but were significantly better at recognizing erotic rather than neutral or training pictures (d = 1.445 and 1.461, respectively, both p < 0.001). This indicates that spatial attention bias to sexual pictures is small, negligible, possibly even non-existent, or else the DPT is not a reliable tool to assess it. Results of the PRT, on the other hand, show that sexual stimuli are prioritized in memory and this should be explored in future research.
Integrated Co-functional Network Analysis on the Resistance and Virulence Features in Acinetobacter baumannii

Acinetobacter baumannii is one of the most troublesome bacterial pathogens that pose major public health threats due to its rapidly increasing drug resistance property. It is not only derived from clinic setting but also emerges from aquaculture as a fish pathogen, which could pass the resistant genes in the food chain. Understanding the mechanism of antibiotic resistance development and pathogenesis will aid our battle with the infections caused by A. baumannii. In this study, we constructed a co-functional network by integrating multiple sources of data from A. baumannii and then used the k-shell decomposition to analyze the co-functional network. We found that genes involving in basic cellular physiological function, including genes for antibiotic resistance, tended to have high k-shell values and locate in the internal layer of our network. In contrast, the non-essential genes, such as genes associated with virulence, tended to have lower k-shell values and locate in the external layer. This finding allows us to fish out the potential antibiotic resistance factors and virulence factors. In addition, we constructed an online platform ABviresDB (https://acba.shinyapps.io/ABviresDB/) for visualization of the network and features of each gene in A. baumannii. The network analysis in this study will not only aid the study on A. baumannii but also could be referenced for the research of antibiotic resistance and pathogenesis in other bacteria.
The Effects of Hormonal Contraceptives on the Brain: A Systematic Review of Neuroimaging Studies

Background: Hormonal contraceptive drugs are being used by adult and adolescent women all over the world. Convergent evidence from animal research indicates that contraceptive substances can alter both structure and function of the brain, yet such effects are not part of the public discourse or clinical decision-making concerning these drugs. We thus conducted a systematic review of the neuroimaging literature to assess the current evidence of hormonal contraceptive influence on the human brain. Methods: The review was registered in PROSPERO and conducted in accordance with the PRISMA criteria for systematic reviews. Structural and functional neuroimaging studies concerning the use of hormonal contraceptives, indexed in Embase, PubMed and/or PsycINFO until February 2020 were included, following a comprehensive and systematic search based on predetermined selection criteria. Results: A total of 33 articles met the inclusion criteria. Ten of these were structural studies, while 23 were functional investigations. Only one study investigated effects on an adolescent sample. The quality of the articles varied as many had methodological challenges as well as partially unfounded theoretical claims. However, most of the included neuroimaging studies found functional and/or structural brain changes associated with the use of hormonal contraceptives. Conclusion: The included studies identified structural and functional changes in areas involved in affective and cognitive processing, such as the amygdala, hippocampus, prefrontal cortex and cingulate gyrus. However, only one study reported primary research on a purely adolescent sample. Thus, there is a need for further investigation of the implications of these findings, especially with regard to adolescent girls.
Longitudinal Links Between Media Use and Focused Attention Through Toddlerhood: A Cumulative Risk Approach

Previous studies that examined the links between media use and children’s attention abilities have yielded inconclusive findings. In the current study, we aimed to move beyond the focus on isolated aspects of media use to a comprehensive assessment of both direct and indirect media use and practices in early childhood. Drawing from the cumulative risk literature, we examined whether cumulative media use is related to children’s subsequent attention abilities. Participants were 199 mothers of toddlers (60% male) who completed questionnaires assessing various aspects of children’s media use, as well as children’s focused attention abilities at three time points: 18 months (T1), 22 months (T2), and 26 months (T3) of age. Cumulative media use scores were computed based on four indicators: (1) child average daily screen time; (2) household background television; (3) maternal use of media to regulate child distress; and (4) maternal use of mobile devices while spending time with the child. An autoregressive cross-lagged (ARCL) path model controlling for child sex, maternal education, and general parenting practices showed that cumulative media use at 18 months negatively predicted children’s focused attention at 22 months. Moreover, there was a significant negative indirect effect from cumulative media use at 18 months to focused attention at 26 months via focused attention at 22 months. Finally, the cumulative media index appeared to be a better predictor of focused attention than any of the singular media use indicators. Children’s focused attention did not predict subsequent cumulative media use across time, providing no evidence for bidirectional links. Findings suggest that exposure to multiple (rather than single) aspects of media use is related to decreased subsequent focused attention abilities during toddlerhood. Family media plans that designate media-free time and increase parental awareness to media use habits in the household should therefore be encouraged.
Epidemiological and Clinical Characteristics of COVID-19 in Children: A Systematic Review and Meta-Analysis

Given the relatively low rate and limited publicly available data regarding children with SARS-CoV-2 infection, this knowledge gap should be addressed with urgency. This systematic review with meta-analysis aimed to evaluate the epidemiological spectrum and clinical characteristics of children infected with SARS-CoV-2. Relevant international and Chinese public databases were systematically searched to identify all case studies from January 1, 2020 to May 7, 2020. This study consisted of 96 studies involving 7004 cases. The mean age of pediatric cases was 6.48 years (95% CI 52.0–77.5), 90% had household contact, and 66% presented with mild to moderate clinical syndromes. The main symptoms were fever (47%, 95% CI 41–53%) and cough (42%, 95% CI 36–48%). About 23% of children were asymptomatic, 27% had comorbidity, and 29% had a co-infection. The pooled mean incubation period was 9.57 days (95% CI 7.70–11.44). The shedding of SARS-CoV-2 in the upper respiratory tract lasted 11.43 days, and 75% of patients had virus particles in their stool. A total of 34% of the children had neutropenia and 26% had lymphocytosis. Interferon-alpha (81%) was the most commonly used antiviral drug in the children. The discharge and death rates were 79 and 1%. In conclusion, the transmissibility of pediatric COVID-19 should be not ignored because of the relatively long incubation period, shedding duration, and mild clinical syndromes.
Survival of LPAI H5N1 in water containing tilapia zillii

The present study aimed to test the survival of LPAI H5N1 in water in the presence of Tilapia zillii fishes, the experiment was conducted using aquarium filled with 45 liters of Nile water and nine fishes of Tilapia zillii. The presence of the virus was tested in water samples by real-time RT-PCR. The viability and the titer of the virus in water samples extracted from aquarium were detected by Plaque infectivity assay and Heamagglutination assay, respectively. The results showed that, the viral RNA persisted 28 days in aquarium water. The CT values showed that the concentration of the virus seemed to be lowered by the time. The virus was survived in water containing fishes Tilapia zillii for 8 days by using plaque assay technique. In water containing Tilapia zillii fishes, the titer of LPAI-H5N1 virus was 512 and 64 HA at zero time and 4 days, respectively. In conclusion, prolonged persistence and infectivity of the virus in water might provide an evidence for the possibility of re-infection of poultry and human who might use or deal with this contaminated water for
No Effect of Long-Term Risedronate Use on Cartilage and Subchondral Bone in an Experimental Rabbit Model of Osteoarthritis

Osteoarthritis (OA) is the most prevalent degenerative joint disease in animals and humans. It is characterized by pain, articular cartilage damage and joint stiffness. It has been suggested that the status of the subchondral bone compartment plays an important role in the initiation and progression of OA. Bisphosphonates have been proposed as a potential disease-modifying treatment for OA, however their effectiveness is not yet clear. Twenty-four male adult New Zealand rabbits were used to evaluate the effects of risedronate on the subchondral bone quality and cartilage degradation in a long-term model of experimentally induced OA. Animals underwent an anterior cruciate ligament transection and partial medial meniscectomy or sham operation in only one knee, which was randomly chosen, using the contralateral as healthy control. Animals were divided into three groups (n = 8): untreated control group and sham surgery control group; both groups received only vehicle; and risedronate group, treated with 2.5 mg orally weekly for 24 weeks. Stifle joints were harvested and scanned using a high-resolution micro-CT to evaluate the subchondral plate and trabecular bone changes. The macroscopic evaluation and histological analysis were determined using an adapted Osteoarthritis Research Society International scoring scheme to assess the cartilage degeneration. The lateral and medial femoral condyle and tibial plateau were evaluated. Additionally, the histological synovial membrane assessment was carried out. Sample analysis showed that the experimental model induced osteoarthritic changes in the operated joints, whereas in sham-operated rabbits, almost no histological changes were observed on articular cartilage surfaces. In terms of macroscopic and histological analyses, risedronate-treated animals did not show improved cartilage health compared with untreated operated rabbits, but a slightly anti-inflammatory activity was observed in the synovial membrane. Risedronate administration showed a slight tendency to increase subchondral bone plate thickness in lateral compartments but, it did not show conservation of periarticular bone and was not be able to suppress the osteophyte formation. In conclusion, long-term risedronate use did not demonstrate a positive effect on reducing the cartilage damage, and failed to prevent the subchondral bone changes and osteophytogenesis in an experimental rabbit model of OA.
The Moderating Effects of Young Adults’ Personality Traits on Social Media Immersion

Young adults are currently among the heaviest users of Internet-based social media applications. The goal of this study was to develop and empirically validate a conceptual model to test associations between students’ attitudes toward social media and their experiences in social media use and immersion. Participants were 9,633 students (average age 16 years; 4,702 males, 4,931 female) who randomly selected from 150 high schools in Taiwan. Participants completed questionnaire surveys describing their attitudes toward social media, immersion experiences, and Big Five personality traits. Structural equation modeling was used to determine factors that predicted and moderated social media immersion. The results of this study highlight the impact that specific personality traits have on the connections between attitudes toward social media and the immersion young adults experience when engaged with social media platforms. These findings suggest that schools and families should establish guidelines to protect young adults from excessive immersion in social media usage, ensure the safety of online environments for this user group, and inform youth regarding the proper use of social media.
Ein neues Darstellungsverfahren fr 2-Aryl-?1-pyrroline und 2-Aryl-?1-piperideine

Ein neues Darstellungsverfahren fur 2-Aryl-Δ1- payrroline bzw. 2-Aryl-Δ1-piperideine durch Umsetzung von γ- bzw. δ-Aminosauren mit aliphatischen Phenol- oder Thiophenol-athern oder mit Phenolen in Polyphosphorsaure wird beschrieben.
Perceived Paternal Involvement, Relationship Satisfaction, Mothers' Mental Health and Parenting Stress: A Multi-Sample Path Analysis

Research has demonstrated the short- and long-term impacts of maternal mental health and well-being on children's emotional and behavioral outcomes. It is thus important to better understand the antecedents of maternal depression and stress. The aim of this study was to determine whether the contribution of perceived paternal involvement to account for mothers' depression and parental stress was mediated by relationship factors such as parenting alliance and dyadic adjustment. A second aim was to determine whether these relationships hold equally true in mothers of infants and young toddlers (0–24 months) and mothers of older children (25 months and older). Cross-sectional data were collected from 447 mothers. Mothers reported on their perceptions of paternal involvement with childcare responsibilities, dyadic adjustment, parenting alliance, parenting stress, and depression. Multi-sample path modeling analyses were conducted. Results revealed that perceived paternal involvement was positively related to both dyadic adjustment and parental alliance, that parenting alliance was negatively related to all three subscales of parenting stress and mothers' depression but that dyadic adjustment was negatively related to parenting distress (one subscale of parenting stress) and mothers' depression. Results from the multi-sample analyses indicated that the pattern of relationships was the same in the two groups, but that the model was not invariant. The most notable difference was that parenting alliance did not significantly account for depression in the mothers of younger children. Correlates of maternal mental health and well-being identified in this study could be useful when designing psychological interventions for mothers and fathers.
CD52 Is a Prognostic Biomarker and Associated With Tumor Microenvironment in Breast Cancer

Tumor microenvironment (TME) plays an essential role in the development and metastasis of breast cancer (BC). More studies are needed on the differences and functions of immune components and matrix components. In this study, we calculated the proportion of tumor-infiltrating immune cells (TICs) and the proportion of immune and matrix components of BC patients from The Cancer Genome Atlas (TCGA). We performed Cox regression analysis and constructed protein-protein interaction (PPI) network based on the differentially expressed genes (DEGs) and obtained the most crucial gene CD52. CD52 significantly upregulated and affected the prognosis of BC patients. Gene set enrichment analysis (GSEA) suggested that the genes in the CD52 high-expression group were mainly enriched in immune-related pathways, while those in the CD52 low-expression group were mainly enriched in metabolic pathways. TICs analyses showed that there should be a positive correlation between CD52 expression and CD8+ T cells, activated memory CD4+ T cells, macrophage M1, and Gamma Delta T cells. It indicated that CD52 might be an essential factor in maintaining the immune-dominant position of TME. These results suggest that CD52 might be a potential biomarker for prognosis and provide a new therapeutic target for BC patients.
Identification of Spindle and Kinetochore-Associated Family Genes as Therapeutic Targets and Prognostic Biomarkers in Pancreas Ductal Adenocarcinoma Microenvironment

Aim The role of spindle and kinetochore-associated (SKA) genes in tumorigenesis and cancer progression has been widely studied. However, so far, the oncogenic involvement of SKA family genes in pancreatic cancer and their prognostic potential remain unknown. Methods Here, we carried out a meta-analysis of the differential expression of SKA genes in normal and tumor tissue. Univariate and multivariate survival analyses were done to evaluate the correlation between SKA family gene expression and pancreas ductal adenocarcinoma (PDAC) prognosis. Joint-effect and stratified survival analysis as well as nomogram analysis were used to estimate the prognostic value of genes. The underlying regulatory and biological mechanisms were identified by Gene set enrichment analysis. Interaction between SKA prognosis-related genes and immune cell infiltration was assessed using the Tumor Immune Estimation Resource tool. Results We find that SKA1–3 are highly expressed in PDAC tissues relative to non-cancer tissues. Survival analysis revealed that high expression of SKA1 and SKA3 independently indicate poor prognosis but they are not associated with relapse-free survival. The prognostic value of SKA1 and SKA3 was further confirmed by the nomogram, joint-effect, and stratified survival analysis. Analysis of underlying mechanisms reveals that these genes influence cancer-related signaling pathways, kinases, miRNA, and E2F family genes. Notably, prognosis-related genes are inversely correlated with several immune cells infiltrating levels. Conclusion We find that SKA1 and SKA3 expression correlates with prognosis and immune cell infiltration in PDAC, highlighting their potential as pancreatic cancer prognostic biomarkers.
An Introduction to the Objective Psychophysics Toolbox

The Psychophysics Toolbox (PTB) is one of the most popular toolboxes for the development of experimental paradigms. It is a very powerful library, providing low-level, platform independent access to the devices used in an experiment such as the graphics and the sound card. While this low-level design results in a high degree of flexibility and power, writing paradigms that interface the PTB directly might lead to code that is hard to read, maintain, reuse, and debug. Running an experiment in different facilities or organizations further requires it to work with various setups that differ in the availability of specialized hardware for response collection, triggering, and presentation of auditory stimuli. The Objective Psychophysics Toolbox (o_ptb) provides an intuitive, unified, and clear interface, built on top of the PTB that enables researchers to write readable, clean, and concise code. In addition to presenting the architecture of the o_ptb, the results of a timing accuracy test are presented. Exactly the same MATLAB code was run on two different systems, one of those using the VPixx system. Both systems showed sub-millisecond accuracy.
Platelet storage and functional integrity

Abstract Platelet transfusion is a topic of common interest for many specialists involved in patient care, from laboratory staff to clinical physicians. Various aspects make this type of transfusion different from those of other blood components. In this review, the challenges in platelet transfusion practice that are relevant for laboratory colleagues will be discussed, highlighting how the biochemical and structural characteristics of these blood elements directly affect their function and consequently the clinical outcome. More than 1,300 platelet concentrates are transfused in Germany every day, and several types are offered by their respective manufacturers. We describe the technological advances in platelet concentrate production, with a focus on how the storage conditions of platelets can be improved. Laboratory quality assessment procedures for a safe transfusion are discussed in detail. For this purpose, we will refer to the Hemotherapy Directives (Richtlinie Hämotherapie) of the German Medical Association.