Miracle-dz/UniFAR-FTUs huggingface dataset:
Improving lung cancer diagnosis by combining exhaled-breath data and clinical parameters

Introduction Exhaled-breath analysis of volatile organic compounds could detect lung cancer earlier, possibly leading to improved outcomes. Combining exhaled-breath data with clinical parameters may improve lung cancer diagnosis. Methods Based on data from a previous multi-centre study, this article reports additional analyses. 138 subjects with non-small cell lung cancer (NSCLC) and 143 controls without NSCLC breathed into the Aeonose. The diagnostic accuracy, presented as area under the receiver operating characteristic curve (AUC-ROC), of the Aeonose itself was compared with 1) performing a multivariate logistic regression analysis of the distinct clinical parameters obtained, and 2) using this clinical information beforehand in the training process of the artificial neural network (ANN) for the breath analysis. Results NSCLC patients (mean±sd age 67.1±9.1 years, 58% male) were compared with controls (62.1±7.0 years, 40.6% male). The AUC-ROC of the classification value of the Aeonose itself was 0.75 (95% CI 0.69–0.81). Adding age, number of pack-years and presence of COPD to this value in a multivariate regression analysis resulted in an improved performance with an AUC-ROC of 0.86 (95% CI 0.81–0.90). Adding these clinical variables beforehand to the ANN for classifying the breath print also led to an improved performance with an AUC-ROC of 0.84 (95% CI 0.79–0.89). Conclusions Adding readily available clinical information to the classification value of exhaled-breath analysis with the Aeonose, either post hoc in a multivariate regression analysis or a priori to the ANN, significantly improves the diagnostic accuracy to detect the presence or absence of lung cancer. Adding readily available clinical information to the classification value of exhaled-breath analysis with the Aeonose significantly improves the diagnostic accuracy to detect the presence or absence of lung cancer http://bit.ly/38ps6fH
Recent advances in epilepsy genomics and genetic testing

Developmental and epileptic encephalopathies (DEEs) are a group of severe, early onset epilepsies characterized by refractory seizures, developmental delay or regression associated with ongoing epileptic activity, and generally poor prognosis. DEE is genetically and phenotypically heterogeneous, and there is a plethora of genetic testing options to investigate the rapidly growing list of epilepsy genes. However, more than 50% of patients with DEE remain without a genetic diagnosis despite state-of-the-art genetic testing. In this review, we discuss the major advances in epilepsy genomics that have surfaced in recent years. The goal of this review is to reach a larger audience and build a better understanding of pathogenesis and genetic testing options in DEE.
Clinical report. Acral melanoma in Cambodia: challenges associated with a rare and aggressive disease encountered in a developing country setting

Abstract Background: Melanoma is a highly malignant skin cancer. Acral lentiginous melanoma (ALM) is most common among Asians. The prevalence of ALM among melanoma cases in Cambodian is not known. Objective: To describe the prevalence of ALM among cases of melanoma and the stage of disease at presentation to the health facilities and health providers. Method: Electronic clinical records were obtained from Children’s Surgical Centre (CSC) database between January 2002 and April 2014. Types and stages melanoma at presentation were documented. Result: A total of 10 out of 26 melanoma patients had ALM. Their average age at diagnosis was 65.3 years. Most primary lesions were located on the plantar or lateral surfaces of the foot. Most histopathological descriptions were consistent with ALM. Seventy percent had lymph node metastases. Conclusion: A high proportion of ALM was found associated with trauma as a potential risk factor. Most frequency presented at a late stage of disease. Providers must be made aware of the disease and render appropriate management early for good outcome.
A new therapeutic target to facilitate radioiodine treatment of breast cancer

The development of new therapeutic strategies for breast cancer is urgently needed. Exploitation of radioiodide uptake by the overexpressed sodium iodide symporter (NIS) has been widely proposed as a novel therapeutic strategy. However, radioiodide uptake is insufficient for tumour destruction. The proto-oncogene PBF binds NIS and inhibits its plasma membrane retention, thereby repressing critical radioiodide uptake in thyroid cancer. This thesis demonstrated that PBF, also upregulated in breast cancer, similarly repressed NIS in breast cancer cells, where phosphorylation of PBF at Y174 was key to NIS interaction and could be disrupted via treatment with the Src inhibitor dasatinib. Mutation of a predicted Src consensus sequence (EEN/AAA170-172AAA) abrogated pY174 PBF and radioiodide uptake repression. In the presence of dasatinib-resistant Src (T341I), dasatinib no longer rescued PBF repression of NIS, indicating that Src specifically mediates PBF phosphorylation. Inhibition of N-myristoylation also significantly increased radioiodide uptake. Combined Src and myristoylation inhibition induced a ~70% increase of radioiodide uptake in the absence of PBF overexpression. Thus, disrupting Src phosphorylation of PBF by targeted mutagenesis and Src kinase inhibition reveal radioiodide uptake into breast cancer cells can be significantly enhanced through therapeutic approaches focused on Src:PBF:NIS and myristoylation, making radioiodide treatment of breast cancer potentially viable.
Polyfunctional, Proinflammatory, Tissue‐Resident Memory Phenotype and Function of Synovial Interleukin‐17A+CD8+ T Cells in Psoriatic Arthritis

Genetic associations imply a role for CD8+ T cells and the interleukin‐23 (IL‐23)/IL‐17 axis in psoriatic arthritis (PsA) and other spondyloarthritides (SpA). IL‐17A+CD8+ (Tc17) T cells are enriched in the synovial fluid (SF) of patients with PsA, and IL‐17A blockade is clinically efficacious in PsA/SpA. This study was undertaken to determine the immunophenotype, molecular profile, and function of synovial Tc17 cells in order to elucidate their role in PsA/SpA pathogenesis.
Students Seeking Health-related Information over Internet

The use of Internet to search information related to health has become a common phenomenon. This article investigates the seeking of health-related information of the college going students over the Internet. The researchers have tested the technology acceptance model (TAM) to determine the behavioural intention of the students to seek health-related information over the Internet. A self-administered questionnaire was distributed among the students who were involved in Internet use. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were applied to the responded data. The results showed that it is the perceived usefulness (PU) which has a direct impact on the behavioural intention of the Internet users to search health-related information over the Internet.
Stature estimation measuring per-cutaneous length of ulna in living subjects

Background: Estimation of stature from bones play an important role in identifying unknown bodies, parts of bodies or skeletal remains. Multiple anthropometric techniques have been used to estimate stature from skeletal remains by anthropologists, anatomists & forensic experts. Since olecranon process and styloid process of ulna are easily felt through the skin, it becomes easy to measure the length of the bone. Objectives: This study was carried out to investigate the relationship between stature and ulna length and to derive a regression formula between the ulna length and height of an individual. Materials and Methods: 300 subjects (150 males & 150 females) aged between 21 and 25 years were included in this study. Measurements of ulna length were taken by using Dividing Calipers and the height was recorded using Stature meter. The data was subjected for statistical analysis using SPSS software Results: A positive correlation between height and ulna length was observed in both sexes and it was statistically significant. Regression equation for stature estimation was formulated using the ulna length for both sexes. Conclusion: Since simple and easily applicable methods are involved in the determination of stature, it is widely used. The present study thus helps in establishing identity of an individual when only some remains of the body are found as in mass disasters, bomb explosions and accidents.
The effectiveness of high-intensity interval training on body composition, cardiorespiratory fitness, and cardiovascular risk factors in children

Abstract Background: No previous systematic review has examined the effect of high-intensity interval training (HIIT) interventions on body composition, cardiometabolic risk factors and cardiorespiratory fitness (CRF) in healthy schoolchildren from 5 to 12 years old. Methods: This study will be conducted by following the guideline of the preferred reporting items for systematic review and meta-analysis protocols. An electronic search in MEDLINE (via PubMed), EMBASE (via Scopus), SPORTDiscus, Cochrane Library and Web of Science databases of all dates from inception will be conducted. We will include randomized controlled trials aimed to assess the effectiveness of HIIT to improve cardiometabolic risk factors, body composition, and CRF in children. Two authors will perform the study selection and data collection; disagreements will be solved by a third reviewer. The methodological quality of studies will be assessed by the Cochrane Collaboration's tool for assessing risk of bias (RoB2). Data analysis and synthesis will be performed by Comprehensive Meta-analysis Software and StataSE software, version 15. Conclusion: The results should be disseminated through publication in a peer-reviewed journal. Since the data used in systematic reviews of this type will be extracted exclusively from published studies, approval form and ethics committee will not be required.
Comparative Electroanalytical Studies of Graphite Flake and Multilayer Graphene Paste Electrodes

In this paper, the fabrication, surface characterisation and electrochemical properties of graphite flake (GFPE) and multilayer graphene (MLGPE) paste electrodes are described. The Raman investigations and scanning electron microscopy were used to analyze and compare structure of both carbon materials. The electroanalytical performance of both electrodes was examined and compared on the basis of the square-wave and cyclic voltammetric behavior of acetaminophen and model redox systems. Results of those studies revealed that GFPE has a larger electroactive surface area and better conductive properties, whilst MLGPE demonstrate better analytical characteristic in case of acetaminophen (AC) determination. AC determination was developed using square wave voltammetry (SWV) and square wave stripping voltammetry (SWSV). For both working electrodes, the process of accumulation enabled us to obtain an extended linear range and to lower the detection limit. In pharmaceutical formulations, AC was determined with good recovery.
Short-Term FIFA 11+ Improves Agility and Jump Performance in Young Soccer Players

Studies dealing with the effectiveness of the Fédération Internationale de Football Association (FIFA) 11+ prevention program to improve performance outcomes in children aged < 14 years are limited. This study aimed to point out the effects of the application of short-term FIFA 11+ warm-up program on physical performance in young football players. Participants were 36 youth male football players, divided into a FIFA 11+ (n = 19; mean (SD) age: 11.15 (0.79) y) and a control group (CG: n = 17; age: 10.87 (0.8) y) and trained for 4 weeks. Before and after the training period, standing long jump performance, agility, repeated sprint ability, sit and reach, and “30–15” intermittent fitness tests were assessed. A mixed ANOVA showed significant differences between the groups in the standing long jump test (FIFA 11+: 5.6% vs. CG: −1.9%) in favor of FIFA 11+ over CG. Additionally, the FIFA 11+ performance of the Illinois agility test was significantly better compared to the CG performance (FIFA 11+: −1.9% vs. CG: 0.03%). The main findings of this study suggest that just 4 weeks of implementation of the FIFA 11+ improves physical performance compared with traditional warm-up routines in young soccer players.
Thoracic electrical impedance tomography in Chinese hospitals: a review of clinical research and daily applications

Chinese scientists and researchers have a long history with electrical impedance tomography (EIT), which can be dated back to the 1980s. No commercial EIT devices for chest imaging were available until the year 2014 when the first device received its approval from the China Food and Drug Administration. Ever since then, clinical research and daily applications have taken place in Chinese hospitals. Up to this date (2019.11) 47 hospitals have been equipped with 50 EIT devices. Twenty-three SCI publications are recorded and a further 21 clinical trials are registered. Thoracic EIT is mainly used in patients before or after surgery, or in intensive care units (ICU). Application fields include the development of strategies for protective lung ventilation (e.g. tidal volume and positive end-expiratory pressure (PEEP) titration, recruitment, choice of ventilation mode and weaning from ventilator), regional lung perfusion monitoring, perioperative monitoring, and potential feedback for rehabilitation. The main challenges for promoting clinical use of EIT are the financial cost and the education of personnel. In this review, the past, present and future of EIT in China are introduced and discussed.
Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii

Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of Hysterothylacium aduncum (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.
Reflectance confocal microscopy in atypical intraepidermal melanocytic proliferation: Two cases with dermoscopic and histologic correlation

Editor, Reflectance confocal microscopy (RCM) is an in vivo, noninvasive technique that enhances the accuracy of melanocytic skin lesion diagnosis compared with clinical observation and dermoscopy alone.1,2 The key RCM features of benign and malignant melanocytic lesions have been described, and the diagnostic criteria for melanoma are well known,3 also for in situ lesions.4 In this context, Atypical Intraepidermal Melanocytic Proliferation (AIMP) is a histologic described entity,5,6 with no clear clinical or dermoscopic differences from a benign nevus or an early melanoma; this term refers to a histologic conceptual diagnosis made when the proliferation is “worse” than a dysplastic nevus, but does not satisfy criteria for melanoma in situ.7,8 As no previous studies highlighted the RCM pattern of this entity, herein we report the confocal features of two patients with proven excisional biopsy of AIMP, with correlation with dermoscopic and histologic findings. The patients were a 78 and a 67-year-old woman with two suspect melanocytic lesions located at the right thigh and right cheek, respectively. Dermoscopy observation (FotoFinder®, FotoFinder Systems GmbH) revealed a 0.3 mm
Claudin-12 Knockout Mice Demonstrate Reduced Proximal Tubule Calcium Permeability

The renal proximal tubule (PT) is responsible for the reabsorption of approximately 65% of filtered calcium, primarily via a paracellular pathway. However, which protein(s) contribute this paracellular calcium pore is not known. The claudin family of tight junction proteins confers permeability properties to an epithelium. Claudin-12 is expressed in the kidney and when overexpressed in cell culture contributes paracellular calcium permeability (PCa). We therefore examined claudin-12 renal localization and its contribution to tubular paracellular calcium permeability. Claudin-12 null mice (KO) were generated by replacing the single coding exon with β-galactosidase from Escherichia coli. X-gal staining revealed that claudin-12 promoter activity colocalized with aquaporin-1, consistent with the expression in the PT. PTs were microperfused ex vivo and PCa was measured. PCa in PTs from KO mice was significantly reduced compared with WT mice. However, urinary calcium excretion was not different between genotypes, including those on different calcium containing diets. To assess downstream compensation, we examined renal mRNA expression. Claudin-14 expression, a blocker of PCa in the thick ascending limb (TAL), was reduced in the kidney of KO animals. Thus, claudin-12 is expressed in the PT, where it confers paracellular calcium permeability. In the absence of claudin-12, reduced claudin-14 expression in the TAL may compensate for reduced PT calcium reabsorption.
Snack food consumption among Bangladeshi children, supplementary data from a large RCT

Abstract Childhood obesity has been associated with consumption of energy‐dense foods such as caloric beverages and fast foods. Many low‐ and middle‐income countries like Bangladesh are now experiencing a rising problem of noncommunicable diseases along with the long‐standing problem of stunting and undernutrition. WASH Benefits Bangladesh was a large community‐based cluster randomized controlled trial conducted in rural Bangladesh. Study clusters were randomized into seven arms: single nutrition (N); water (W); sanitation (S); hygiene (H); combined water, sanitation, and hygiene (WSH); WSH and nutrition (N + WSH); and a double sized control (C). Nutrition intervention messages included four promotional components: maternal nutrition, breastfeeding, complementary feeding, and lipid‐based nutrient supplements. The World Health Organization infant food frequency questionnaire (24‐hr recall and 7‐day recall) was administered at Year 1 and Year 2 of intervention. The likelihood of any snack food consumption was significantly lower (odds ratio 0.37: 95% confidence interval [0.28, 0.49]) in the nutrition intervention arms compared to the control arm in Year 2 follow‐up. In addition, in the water intervention arm, fewer children (about 50% less) consumed soft drinks, but not the other sugar‐sweetened beverages, compared with control in Year 2. There were no other differences between groups. Simple messages about balanced diet and feeding family foods were effective in lowering commercially produced snack food consumption of the young children in low‐income rural communities of Bangladesh. Provision of safe water apparently encouraged mothers to reduce offering unhealthy beverages to the young children.
CYD Tetravalent Dengue Vaccine Performance by Baseline Immune Profile (Monotypic/Multitypic) in Dengue-Seropositive Individuals

Abstract Background The immune profile of dengue-experienced individuals is a determinant of dengue reinfection severity risk. Individuals with a single prior dengue infection (monotypic) are at highest risk for severe disease, while individuals with ≥ 2 prior dengue infections (multitypic) are at lower risk. The tetravalent dengue vaccine (CYD-TDV) has shown efficacy in the prevention of dengue in individuals with prior dengue infection. We estimated efficacy in individuals with monotypic or multitypic immune profiles. Methods Participants enrolled in the immunogenicity subsets of 2 randomized placebo-controlled phase 3 studies (CYD14, NCT01373281; CYD15, NCT01374516) were classified as either monotypic or multitypic, based on measured baseline dengue plaque reduction neutralization test. Vaccine efficacy (VE) against symptomatic virologically confirmed dengue (VCD) was assessed over 25 months and against VCD hospitalization over 6 years. Results Of 3927 participants in the immunogenicity subsets, 496 and 257 in the CYD-TDV and placebo groups, respectively, were classified as monotypic immune, and 1227 and 612, respectively, as multitypic immune. VE against symptomatic VCD was 77.4% (95% CI, 56.4%–88.2%) for monotypic and 89.2% (95% CI, 71.5%–95.9%) for multitypic profiles, with corresponding absolute risk reductions (ARRs) of 4.48% (95% CI, 2.32%–6.65%) for monotypics and 1.67% (95% CI, .89%–2.46%) for multitypics. VE against hospitalized VCD was 75.3% (95% CI, 42.7%–90.2%) in monotypics and 81.2% (95% CI, 21.7%–96.8%) in multitypics, with ARRs of 0.95% (95% CI, .37%–1.53%) for monotypics and 0.18% (95% CI, .02%–.34%) for multitypics. Conclusions CYD-TDV benefits individuals with monotypic and multitypic immune profiles. Larger public health benefit is expected to derive from the protection of individuals with a monotypic immune profile.
Mutational analysis indicates that abnormalities in rhizobial infection and subsequent plant cell and bacteroid differentiation in pea (Pisum sativum) nodules coincide with abnormal cytokinin responses and localization.

BACKGROUND AND AIMS Recent findings indicate that Nod factor signalling is tightly interconnected with phytohormonal regulation that affects the development of nodules. Since the mechanisms of this interaction are still far from understood, here the distribution of cytokinin and auxin in pea (Pisum sativum) nodules was investigated. In addition, the effect of certain mutations blocking rhizobial infection and subsequent plant cell and bacteroid differentiation on cytokinin distribution in nodules was analysed. METHODS Patterns of cytokinin and auxin in pea nodules were profiled using both responsive genetic constructs and antibodies. KEY RESULTS In wild-type nodules, cytokinins were found in the meristem, infection zone and apical part of the nitrogen fixation zone, whereas auxin localization was restricted to the meristem and peripheral tissues. We found significantly altered cytokinin distribution in sym33 and sym40 pea mutants defective in IPD3/CYCLOPS and EFD transcription factors, respectively. In the sym33 mutants impaired in bacterial accommodation and subsequent nodule differentiation, cytokinin localization was mostly limited to the meristem. In addition, we found significantly decreased expression of LOG1 and A-type RR11 as well as KNOX3 and NIN genes in the sym33 mutants, which correlated with low cellular cytokinin levels. In the sym40 mutant, cytokinins were detected in the nodule infection zone but, in contrast to the wild type, they were absent in infection droplets. CONCLUSIONS In conclusion, our findings suggest that enhanced cytokinin accumulation during the late stages of symbiosis development may be associated with bacterial penetration into the plant cells and subsequent plant cell and bacteroid differentiation.