Miracle-dz/UniFAR-FTUs huggingface dataset:
Mécanismes épigénétiques et réponse des cellules cancéreuses au microenvironnement : implication de la méthylation de l’ADN et de l’un de ses interprètes, MBD2

Les cancers du sein ont la particularite de developper un microenvironnement tumoral important ou les fibroblastes associes au cancer (CAF) jouent un role crucial dans la tumorigenese via la secretion de differents facteurs de croissance, cytokines, proteases et composants de la matrice extracellulaire. Ces differents facteurs secretes par les CAFs sont impliques dans de nombreuses voies de signalisation suggerant que la reprogrammation des cellules cancereuses par les CAFs peut affecter de nombreux genes.Le sequencage des ARN messagers (RNAseq) de lignees cellulaires de cancer du sein cultivees en presence de milieux conditionnes de CAF, nous a permis d'identifier 372 genes surexprimes in vitro par les facteurs secretes par les CAF et in vivo selon la teneur en cellules stromales des tumeurs mammaires. De facon inattendue, nous avons pu constater que les facteurs secretes par les CAF ainsi que le contenu en cellules stromales des tumeurs n'induisent pas de changements significatifs de methylation de l'ADN mais activent de maniere specifique des genes caracterises par une signature methylation. Differentes approches experimentales, telles que l'inhibition de la methylation de l'ADN, l'inhibition de l'expression de la proteine MBD2 (proteines de liaison a l'ADN methyle) et des experiences d'immuno-precipitation de la chromatine (ChIP) ont permis de montrer l'implication de ces marques de methylation et des proteines de liaison a l'ADN methyle dans la reponse des cellules cancereuses aux facteurs secretes par les CAFs. Ces resultats ont permis l'identification d'evenements moleculaires impliques dans la reponse des cellules tumorales aux signaux secretes par les cellules stromales dans les tumeurs mammaires mettant en lumiere l'importance des marques epigenetiques dans la reprogrammation des cellules cancereuses induites par les cellules stromales
Phosphatidic acid‐PKA signaling regulates p38 and ERK1/2 functions in ligand‐independent EGFR endocytosis

Ligand‐independent epidermal growth factor receptor (EGFR) endocytosis is inducible by a variety of stress conditions converging upon p38 kinase. A less known pathway involves phosphatidic acid (PA) signaling toward the activation of type 4 phosphodiesterases (PDE4) that decrease cAMP levels and protein kinase A (PKA) activity. This PA/PDE4/PKA pathway is triggered with propranolol used to inhibit PA hydrolysis and induces clathrin‐dependent and clathrin‐independent endocytosis, followed by reversible accumulation of EGFR in recycling endosomes. Here we give further evidence of this signaling pathway using biosensors of PA, cAMP, and PKA in live cells and then show that it activates p38 and ERK1/2 downstream the PKA inhibition. Clathrin‐silencing and IN/SUR experiments involved the activity of p38 in the clathrin‐dependent route, while ERK1/2 mediates clathrin‐independent EGFR endocytosis. The PA/PDE4/PKA pathway selectively increases the EGFR endocytic rate without affecting LDLR and TfR constitute endocytosis. This selectiveness is probably because of EGFR phosphorylation, as detected in Th1046/1047 and Ser669 residues. The EGFR accumulates at perinuclear recycling endosomes colocalizing with TfR, fluorescent transferrin, and Rab11, while a small proportion distributes to Alix‐endosomes. A non‐selective recycling arrest includes LDLR and TfR in a reversible manner. The PA/PDE4/PKA pathway involving both p38 and ERK1/2 expands the possibilities of EGFR transmodulation and interference in cancer.
Nocturnal Enuresis in Sickle Cell: Sociodemographic, Medical, and Quality of Life Factors.

OBJECTIVE Nocturnal enuresis is more prevalent in youth with sickle cell disease (SCD) compared to the general population. The purpose of this study is to estimate prevalence of nocturnal enuresis using diagnostic criteria and identify associated sociodemographic, medical, and health-related quality of life (HRQOL) factors. METHODS Youth with SCD (N = 248; ages 6.00-17.99 years) and their caregivers completed semi-structured interviews and questionnaires. HRQOL was measured using the Pediatric Quality of Life (PedsQL) Inventory. Medical information was abstracted from medical record. We generated multivariable logistic regression models to examine associations between factors and current nocturnal enuresis and nocturnal enuresis occurring any time in the past (lifetime). RESULTS Among participants (mean age, 11.3 ± 3.6 years; 50.8% male), 21.4% reported current nocturnal enuresis and 46% reported lifetime nocturnal enuresis. Male sex [odds ratio (OR), 2.57; p = . 001], difficulty arousing from sleep (OR, 3.57; p < .001), higher school functioning HRQOL (OR, 1.02; p = .014), and higher fetal hemoglobin levels (OR, 1.03; p = .048) were associated with lifetime nocturnal enuresis. Younger age (OR, 1.16; p = .005), higher youth-reported fatigue (OR, 1.01; p = .045), difficulty arousing from sleep (OR, 4.92; p < .001), and higher lactate dehydrogenase levels (OR, 1.00; p = .042) were associated with current nocturnal enuresis. CONCLUSIONS Nocturnal enuresis is prevalent in youth with SCD and is associated with HRQOL, diminished sleep, greater fatigue, and disease severity markers. Routine assessment of sleep behaviors and fatigue are necessary when treating patients with SCD to understand the impact of nocturnal enuresis on HRQOL.
Optimised and defined method for isolation and preservation of precursor cells from human umbilical cord

The present invention relates to an optimized method is defined to separate and preserve the progenitor cells from human umbilical cord. In addition to reproducibly and that with 100% reliability, and in terms of the of the processed samples, the higher is the method gets the progenitor cell of a prescribed number, the majority thereof is single in a short time frame than the one described in the prior art, the prior adhesion and is obtained after the expansion / growth step. In the context of the present method, after direct refrigeration of the cell fraction, and most of the single in vitro expansion / growth phase of the cell after (the end of the P0), the cord 1 of about 8.6 (± 0.1) × 1O per gram processed in about 9 hours it is possible to obtain the In addition, the properties of the cell it is possible for example to obtain a 35-day, the average from the processed cord of 100% with precursor phenotype 7.7 × 1O The method is simple, reliably, because there is 100% reliable and good manufacturing laboratory standard in provided in common for the human cell therapy; can be carried out (good manufacturing practice GMP). In addition, the present method is applied in the pharmaceutical, cosmetics and biotechnology fields.
Impaired test performance yet spared neurocognitive functioning in individuals with obsessive-compulsive disorder: the role of performance mediators

ABSTRACT Introduction Although most studies report neurocognitive deficits in patients with obsessive-compulsive disorder (OCD), important exceptions exist, highlighting the possible role of mediators (e.g., poor motivation). This study investigated neurocognitive functioning and potential influences affecting performance in OCD. Methods Forty-three participants (13 OCD patients, 30 healthy controls) were assessed using a battery of neurocognitive tests. During the assessment, the examiner completed the Impact on Performance Scale (IPS) which measures variables that may impact neurocognitive performance. Results Pooled neurocognitive performance was lower in OCD patients versus healthy controls at a moderate effect size. Patients performed more poorly on the IPS, particularly the Well-Being During Assessment subscale. Performance differences across the two groups were attenuated to a non-significant small-to-medium effect when the IPS was entered as a covariate. A total of 34% of patients showed scores greater than one standard deviation below the mean compared to 9.63% in healthy individuals. Yet, when a conservative impairment criterion (≥2 standard deviations below the mean) was applied, less than 10% of patients displayed deficits. Conclusions Neurocognitive impairment in OCD is likely exaggerated. In addition to considering important mediators researchers should report the percentage of participants displaying performance deficits rather than mean group differences alone; the latter obscures the high percentage of patients without impairment and thus may unduly foster stigma in this population.
Applications of bacteriophages against intracellular bacteria

Abstract Infectious diseases pose a significant threat to both human and animal populations. Intracellular bacteria are a group of pathogens that invade and survive within the interior of eukaryotic cells, which in turn protect them from antibacterial drugs and the host immune system. Limited penetration of antibacterials into host cells results in insufficient bacterial clearance and treatment failure. Bacteriophages have, over the decades, been proved to play an important role in combating bacterial infections (phage therapy), making them an important alternative to classical antibiotic strategies today. Phages have been found to be effective at killing various species of extracellular bacteria, but little is still known about how phages control intracellular infections. With advances in phage genomics and mechanisms of delivery and cell uptake, the development of phage-based antibacterial strategies to address the treatment of intracellular bacteria has general potential. In this review, we present the current state of knowledge regarding the application of bacteriophages against intracellular bacteria. We cover phage deployment against the most common intracellular pathogens with special attention to therapeutic and preventive strategies.
mTORC1 Promotes ARID1A Degradation and Oncogenic Chromatin Remodeling in Hepatocellular Carcinoma

mTOR promotes oncogenic chromatin remodeling by controlling ARID1A degradation, which is important for liver tumorigenesis and response to mTOR- and YAP-targeted therapies in hepatocellular carcinoma. The SWI/SNF chromatin remodeling complexes control accessibility of chromatin to transcriptional and coregulatory machineries. Chromatin remodeling plays important roles in normal physiology and diseases, particularly cancer. The ARID1A-containing SWI/SNF complex is commonly mutated and thought to be a key tumor suppressor in hepatocellular carcinoma (HCC), but its regulation in response to oncogenic signals remains poorly understood. mTOR is a conserved central controller of cell growth and an oncogenic driver of HCC. Remarkably, cancer mutations in mTOR and SWI/SNF complex are mutually exclusive in human HCC tumors, suggesting that they share a common oncogenic function. Here, we report that mTOR complex 1 (mTORC1) interact with ARID1A and regulates ubiquitination and proteasomal degradation of ARID1A protein. The mTORC1–ARID1A axis promoted oncogenic chromatin remodeling and YAP-dependent transcription, thereby enhancing liver cancer cell growth in vitro and tumor development in vivo. Conversely, excessive ARID1A expression counteracted AKT-driven liver tumorigenesis in vivo. Moreover, dysregulation of this axis conferred resistance to mTOR-targeted therapies. These findings demonstrate that the ARID1A–SWI/SNF complex is a regulatory target for oncogenic mTOR signaling, which is important for mTORC1-driven hepatocarcinogenesis, with implications for therapeutic interventions in HCC. Significance: mTOR promotes oncogenic chromatin remodeling by controlling ARID1A degradation, which is important for liver tumorigenesis and response to mTOR- and YAP-targeted therapies in hepatocellular carcinoma. See related commentary by Pease and Fernandez-Zapico, p. 5608
Mitogenomes of Accipitriformes and Cathartiformes Were Subjected to Ancestral and Recent Duplications Followed by Gradual Degeneration

Abstract The rearrangement of 37 genes with one control region, firstly identified in Gallus gallus mitogenome, is believed to be ancestral for all Aves. However, mitogenomic sequences obtained in recent years revealed that many avian mitogenomes contain duplicated regions that were omitted in previous genomic versions. Their evolution and mechanism of duplication are still poorly understood. The order of Accipitriformes is especially interesting in this context because its representatives contain a duplicated control region in various stages of degeneration. Therefore, we applied an appropriate PCR strategy to look for duplications within the mitogenomes of the early diverged species Sagittarius serpentarius and Cathartiformes, which is a sister order to Accipitriformes. The analyses revealed the same duplicated gene order in all examined taxa and the common ancestor of these groups. The duplicated regions were subjected to gradual degeneration and homogenization during concerted evolution. The latter process occurred recently in the species of Cathartiformes as well as in the early diverged lineages of Accipitriformes, that is, Sagittarius serpentarius and Pandion haliaetus. However, in other lineages, that is, Pernis ptilorhynchus, as well as representatives of Aegypiinae, Aquilinae, and five related subfamilies of Accipitriformes (Accipitrinae, Circinae, Buteoninae, Haliaeetinae, and Milvinae), the duplications were evolving independently for at least 14–47 Myr. Different portions of control regions in Cathartiformes showed conflicting phylogenetic signals indicating that some sections of these regions were homogenized at a frequency higher than the rate of speciation, whereas others have still evolved separately.
Meanings of participation in care for older people after hip fracture surgery and nurses working in an orthopaedic ward

ABSTRACT Purpose The aim of this study was to elucidate meanings of participation in care for older people after hip fracture surgery and nurses working in an orthopaedic ward. Methods A qualitative phenomenological hermeneutical design was used. We conducted personal interviews with a narrative approach with 11 older people recovering from hip fracture surgery and 12 nurses working in an orthopaedic ward. Results The results show that for older people, participation meant being a co-creator in their own care, founded on being met with sensitivity and support, being told what is going to happen, taking responsibility and asking questions and being able to influence care. For nurses, patient participation meant meeting the patients’ needs and requests by being open and allowing them to influence care while at the same time recognizing that the patients’ possibility to influence care was limited. Conclusion The study shows that for older people and nurses, the phenomenon of participation has similar meanings but also differences. When older people participate in their care, they become a co-creator in care and confirmed as a person. This highlights the importance of a nurse-patient relationship built on trust, connectedness and communication based on a shared understanding.
A sort-seq approach to the development of single fluorescent protein biosensors

The utility of single fluorescent protein biosensors (SFPBs) in biological research is offset by the difficulty in engineering these tools. SFPBs generally consist of three basic components: a circularly permuted fluorescent protein, a ligand-binding domain, and a pair of linkers connecting the two domains. In the absence of predictive methods for biosensor engineering, most designs combining these three components will fail to produce allosteric coupling between ligand binding and fluorescence emission. Methods to construct libraries of biosensor designs with variations in the site of GFP insertion and linker sequences have been developed, however, our ability to construct new variants has exceeded our ability to test them for function. Here, we address this challenge by applying a massively parallel assay termed “sort-seq” to the characterization of biosensor libraries. Sort-seq combines binned fluorescence-activated cell sorting, next-generation sequencing, and maximum likelihood estimation to quantify the dynamic range of many biosensor variants in parallel. We applied this method to two common biosensor optimization tasks: choice of insertion site and optimization of linker sequences. The sort-seq assay applied to a maltose-binding protein domain-insertion library not only identified previously described high-dynamic-range variants but also discovered new functional insertion-sites with diverse properties. A sort-seq assay performed on a pyruvate biosensor linker library expressed in mammalian cell culture identified linker variants with substantially improved dynamic range. Machine learning models trained on the resulting data can predict dynamic range from linker sequence. This high-throughput approach will accelerate the design and optimization of SFPBs, expanding the biosensor toolbox.
Endoscopic Removal of a Fecalith or Fecomucolith in a Rectal Stump or Pouch

Fecaliths or fecomucoliths can form in rectal stumps after a Hartmann procedure or in a coloneovaginal conduit. They can cause significant distress to the patient by causing symptoms such as discharge and odor. We describe a novel and effective method of endoscopic obliteration and removal where other techniques such as removal during examination under anesthesia or serial enemas have failed. By using a combination of hydrodissection with a saline injector gun and biopsy forceps and a polypectomy snare to break down the fecomucolith or fecalith, this troublesome problem can be resolved endoscopically. Successful removal of the fecalith/fecomucolith and resolution of the symptoms for the patients were achieved. No complications are reported. We describe an effective and novel method for endoscopic removal of fecaliths and fecomucoliths.
The Backup Planning Scale (BUPS): A Brief, Self-Reported Measure of a Person’s Tendency to Develop, Reserve, and Use Backup Plans

Abstract To mitigate uncertainty in their goal pursuits, people use backup plans, i.e., alternative means that are developed to potentially replace “Plan A. ” Several studies have demonstrated that backup plans can introduce unexpected costs into goal pursuits that decrease a person’s motivation to continue using their “Plan A,” and reduce their chances for achieving their goal. These existing studies used time-intensive experimental and/or observational approaches to assess the effects of backup planning. The present research examines the newly-developed Backup Planning Scale (BUPS) for its measurement invariance, reliability, validity, and other psychometric characteristics across three independent samples with more than 1,500 participants. Consistent with prior theorizing, we found support for a nine-item, three factor structure for the BUPS, indexing latent factors for a person’s tendency to develop, reserve, and replace with (or use) backup plans. Furthermore, a novel “IRTree” based statistical technique provided evidence for the validity of the measure, as participants’ responses to the BUPS were associated with their actual developing, reserving, and replacing backup planning behaviors in a logic task. We conclude that the freely-available BUPS is a simple, brief, reliable, and valid self-reported instrument for assessing backup planning behaviors across adulthood.
Cellular Communication Propagation at Drone around Building Environment with Single Knife Edge at 10 GHz

The drone communication systems used a cellular network for controlling a drone from a long distance. That communication propagations between drone and base station were analyzed. The drone moved at the track around building environment. That environment used variations in building height. The communication propagation around building environment caused diffraction mechanism. Single knife edge method is used for that diffraction mechanism. The frequency of communication used 10 GHz. That frequency was influenced by atmospheric attenuation. This research was using some variations such as height of drone track location, transmitter power, and AMC (Adaptive Modulation Coding). MCS (Modulation Coding Scheme) was used AMC such as QPSK, 16 QAM, and 64 QAM. Some result was obtained at this research consist of LOS and NLOS distance, SNR, MCS probability, and percentage of drone coverage. NLOS propagation was caused by building height. The SNR value become increase when higher at drone position, such as drone was moving at 20 meters with height of flying drone 80 m and transmitter power 30 dBm obtained SNR 38.21 dBm. That SNR is affected AMC, so a higher SNR value increases AMC. The drone’s coverage 100%, with a height of flying drone 80 meters and transmitter power of 30 dBm. That condition showed more increasing coverage percentage than 64.8% for height of flying drone 20 meters and transmitter power 30 dBm. That result showed that more drone height increased of coverage percentage, probability modulation, and SNR value.
Neural correlates of contributions of interpreting training to second language semantic integration

How interpreting training may enhance semantic integration in general language processing (instead of language processing during interpreting) was not clear, and its neural correlates remained unexplored. Two groups of university students with different amounts of interpreting training experience (more-IE and less-IE) performed a second language (L2) reading task, in which the target sentence ended with three types of lateralized words: expected words, words of related semantic violation (related violation), and words of unrelated semantic violation (unrelated violation). N400 responses elicited by the final words were used to index semantic integration. Two major findings suggested potential contributions of interpreting training to L2 semantic integration. First, the more-IE group exhibited smaller N400 amplitudes than the less-IE group for expected words, and no group differences were obtained for the two violations. Second, N400 amplitudes for related violations were generally smaller than those for unrelated violations in the right visual field, and this difference was more fully captured (more broadly distributed over the scalp) in the more-IE group than in the less-IE group. Therefore, smaller N400 amplitudes for expected words but not for violations, and broader scalp distribution of the differences between related and unrelated violations constitute the neural correlates for the potential contributions of interpreting training to general L2 semantic integration. The results also suggest possible connections between different types of language experience.