Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of the Putative Acylated Cellulose Synthase Operon in Komagataeibacter xylinus E25

Bacterial cellulose is a natural polymer with an expanding array of applications. Because of this, the main cellulose producers of the Komagataeibacter genus have been extensively studied with the aim to increase its synthesis or to customize its physicochemical features. Up to now, the genetic studies in Komagataeibacter have focused on the first cellulose synthase operon (bcsI) encoding the main enzyme complex. However, the role of other accessory cellulose operons has been understudied. Here we aimed to fill this gap by performing a detailed analysis of the second cellulose synthase operon (bcsII), which is putatively linked with cellulose acylation. In this study we harnessed the genome sequence, gene expression and protein structure information of K. xylinus E25 and other Komagataeibacter species to discuss the probable features of bcsII and the biochemical function of its main protein products. The results of our study support the previous hypothesis that bcsII is involved in the synthesis of the acylated polymer and expand it by presenting the evidence that it may also function in the regulation of its attachment to the cell surface and to the crystalline cellulose fibers.
The efficacy and safety of paravertebral block for postoperative analgesia in renal surgery: A systematic review and meta-analysis of randomized controlled trials

Background Paravertebral block (PVB) has been widely used in postoperative analgesia, especially in thoracic and breast surgery. However, the efficacy and safety of PVB for analgesia after renal surgery remains uncertain. Therefore, this study aimed to determine the postoperative analgesic efficacy and safety of PVB in renal surgery. Methods PubMed, Web of Science, Embase, and the Cochrane Library databases were systematically searched up to December 20, 2021. All randomized controlled trials (RCTs) evaluating the postoperative analgesic efficacy of PVB in renal surgery were collected. The meta-analysis was performed using RevMan 5.4 and Stata/MP 14.0 software. Results A total of 16 RCTs involving 907 patients were included in the meta-analysis. Ten studies investigated patients under percutaneous nephrolithotomy (PCNL), and six studies were done for patients under other renal surgery (nephrectomy or pyeloplasty). Compared with control groups (no block, sham block, or other nerve blocks), meta-analysis showed that PVB reduced 24-hour postoperative opioid consumption significantly (SMD = −0.99, 95%CI: −1.60–0.38, p = 0.001, I2 = 92%) and reduced pain scores at various time points within 24 h at rest and 1 h, 4 h, and 24 h at movement after renal surgery, furthermore, PVB prolonged the time to first postoperative analgesic requirement (SMD = 2.16, 95%CI: 0.94–3.39, p = 0.005, I2 = 96%) and reduced the incidence of postoperative additional analgesia (OR = 0.14, 95%CI: 0.06∼0.33, p < 0.00001, I2 = 50%). Subgroup analysis revealed that the postoperative analgesia effect of PVB was more significant in PCNL, and the use of bupivacaine for PVB seemed to have a better performance. Besides, there was no difference in the incidence of postoperative nausea, vomiting, and itching between PVB and control groups. Conclusion This study indicates that PVB may provide effective postoperative analgesia in patients under renal surgery, especially PCNL patients. Moreover, PVB is a safe analgesic method without significant analgesia-related complications.
An Objective Quality Control of Surface Contamination Observations for ABI Water Vapor Radiance Assimilation

A quality control (QC) process which handles surface impacts is an important step toward successful assimilation of the Advanced Baseline Imager (ABI) water vapor (WV) band radiances. If the QC is too relaxed, many surface contaminated radiances get assimilated. If the QC is too stringent, useful radiances are rejected. Either way can result in reduced or even compromised observation impacts. A new machine learning‐based QC scheme for the three ABI WV bands is developed and optimized to help understand the importance and effectiveness of the scheme. Unlike previous schemes which are dependent on the background, this scheme extracts and blends the surface information from 7 ABI bands (bands 8–10, 13–16) to determine if a WV radiance is affected by the surface. Simulation studies show that the new QC scheme is effective in retaining radiances that are either unaffected by the surface or have very small surface contamination. It is highly effective in rejecting radiances with large surface contamination. Numerical experiments from a single case study of Hurricane Harvey (2017) were carried out to optimize the QC and to understand the potential impacts on forecasts. The use of the new QC scheme shows that radiances from each WV band have substantially added value. Combining them has a positive impact on hurricane track forecasts compared with existing QC schemes. Hence, it is critical that an optimized QC scheme is used for infrared WV radiance assimilation. It provides a balance between positive impacts from useful radiances and negative impacts from surface contaminated radiances.
Low Dispersion On-Chip Hollow Waveguide for High Data Rate Applications

Low loss structures for guiding light pulses for on chip communication is the major challenge for the application of high data rate. As time is moving ahead high data rates are becoming the un-escapable for transfer of large data on chip and to overcome the interconnection bottle-neck. In an effort to move this important issue along, the thesis work mainly concentrates on the analysis of engineering of hollow waveguides for being used at higher data rates. Hollow waveguides engineered with high contrast gratings (HCG) have been comprehensively studied over the years due to its diverse application in optoelectronics devices. One of the innovative forms of grating is high-index contrast grating (HCG). It is possible to control the dispersion loss of light in waveguide by optimizing the HCG parameters. Review of the recent advances in HCG grating and its application for high data rate optoelectronic devices is done. From application point of view the dissertation pave the way for realizing low dispersion for high data-rate transmission. A proficient structure of HCG based hollow waveguide (HWG) on SOI is proposed. The presence of three material i.e. silicon-air-silicon oxide grating on top silicon layer in SOI allows a strong interaction between the guided mode and the grating. The design of high data rate hollow waveguide is presented by optimizing the various grating parameters like grating thickness (tg), grating period (˄), waveguide core thickness (d) and refractive index contrast of grating. Optimizing all the parameters the design is then analysed over a wide range of data rates. Design and simulation of waveguide is done in MATLAB using RCWA (Rigorous Coupled wave analysis) and SVFD (Semei-Vectorial Finite Difference) method. Dispersion of 15.14 ps/nm/km is observed at data rate of 40 gbps. The proposed characteristics of the device arise from the engineered structure of hollow waveguide.
Leaf nitrogen from the perspective of optimal plant function

Leaf dry mass per unit area (LMA), carboxylation capacity (Vcmax) and leaf nitrogen per unit area (Narea) and mass (Nmass) are key traits for plant functional ecology and ecosystem modelling. There is however no consensus about how these traits are regulated, or how they should be modelled. Here we confirm that observed leaf nitrogen across species and sites can be estimated well from observed LMA and Vcmax at 25°C (Vcmax25). We then test the hypothesis that global variations of both quantities depend on climate variables in specific ways that are predicted by leaf‐level optimality theory, thus allowing both Narea to be predicted as functions of the growth environment. A new global compilation of field measurements was used to quantify the empirical relationships of leaf N to Vcmax25 and LMA. Relationships of observed Vcmax25 and LMA to climate variables were estimated, and compared to independent theoretical predictions of these relationships. Soil effects were assessed by analysing biases in the theoretical predictions. LMA was the most important predictor of Narea (increasing) and Nmass (decreasing). About 60% of global variation across species and sites in observed Narea, and 31% in Nmass, could be explained by observed LMA and Vcmax25. These traits, in turn, were quantitatively related to climate variables, with significant partial relationships similar or indistinguishable from those predicted by optimality theory. Predicted trait values explained 21% of global variation in observed site‐mean Vcmax25, 43% in LMA and 31% in Narea. Predicted Vcmax25 was biased low on clay‐rich soils but predicted LMA was biased high, with compensating effects on Narea. Narea was overpredicted on organic soils. Synthesis. Global patterns of variation in observed site‐mean Narea can be explained by climate‐induced variations in optimal Vcmax25 and LMA. Leaf nitrogen should accordingly be modelled as a consequence (not a cause) of Vcmax25 and LMA, both being optimized to the environment. Nitrogen limitation of plant growth would then be modelled principally via whole‐plant carbon allocation, rather than via leaf‐level traits. Further research is required to better understand and model the terrestrial nitrogen and carbon cycles and their coupling.
Resveratrol Reverts Tolerance and Restores Susceptibility to Chlorhexidine and Benzalkonium in Gram-Negative Bacteria, Gram-Positive Bacteria and Yeasts

The spread of microorganisms causing health-care associated infection (HAI) is contributed to by their intrinsic tolerance to a variety of biocides, used as antiseptics or disinfectants. The natural monomeric stilbenoid resveratrol (RV) is able to modulate the susceptibility to the chlorhexidine digluconate (CHX) biocide in Acinetobacter baumannii. In this study, a panel of reference strains and clinical isolates of Gram-negative bacteria, Gram-positive bacteria and yeasts were analyzed for susceptibility to CHX and benzalkonium chloride (BZK) and found to be tolerant to one or both biocides. The carbonyl cyanide m-chlorophenylhydrazine protonophore (CCCP) efflux pump inhibitor reduced the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CHX and BZK in the majority of strains. RV reduced dose-dependently MIC and MBC of CHX and BZK biocides when used as single agents or in combination in all analyzed strains, but not CHX MIC and MBC in Pseudomonas aeruginosa, Candida albicans, Klebsiella pneumoniae, Stenotrophomonas maltophilia and Burkholderia spp. strains. In conclusion, RV reverts tolerance and restores susceptibility to CHX and BZK in the majority of microorganisms responsible for HAI. These results indicates that the combination of RV, CHX and BZK may represent a useful strategy to maintain susceptibility to biocides in several nosocomial pathogens.
Vertical or horizontal: optimal integration strategy under separation of ownership and control

This study proposes a framework to analyze the strategic value of integration, when separation of ownership and control is considered. According to this framework, we investigate the optimal integration strategy in different control distribution and discuss how external market influences the best strategic choice. Beside theoretical analysis, we also provide evidence from Chinese listed firms to validate our research. The key results of our study show that first, unintegrated strategy works best, if the level of control-ownership disparity is extremely high. Second, in general, vertical strategy is more appropriate for substitute markets, while horizontal strategy may bring more benefits in a highly complementary market. Third, the impact of integration strategy on a firm ’ s performance is negatively moderated by control-ownership disparity. However, this moderate effect may be weakened by market structure. Our results provide a new and comprehensive perspective for understanding the inconsistent results from previous studies. Moreover, the analysis in this study also highlights a firm ’ s strategic decision and market regulation policy.
Salvage versus Primary Liver Transplantation for Hepatocellular Carcinoma: A Twenty-Year Experience Meta-Analysis

Simple Summary Primary liver transplantation (PLT) for HCC represents the ideal treatment. However, since organ shortage increases the risk of drop-out from the waiting list for tumor progression, a new surgical strategy has been developed: Salvage Liver Transplantation (SLT) can be offered as an additional curative strategy for HCC recurrence after liver resection. The aim of this updated meta-analysis is to compare surgical and long-term outcomes of SLT versus PLT for HCC. The findings of our analysis reveal that SLT offers comparable surgical outcomes but slightly poorer oncological long-term outcomes with respect to PLT. Abstract (1) Background: Primary liver transplantation (PLT) for HCC represents the ideal treatment. However, since organ shortage increases the risk of drop-out from the waiting list for tumor progression, a new surgical strategy has been developed: Salvage Liver Transplantation (SLT) can be offered as an additional curative strategy for HCC recurrence after liver resection. The aim of this updated meta-analysis is to compare surgical and long-term outcomes of SLT versus PLT for HCC. (2) Materials and Methods: A systematic review and meta-analysis was conducted using the published papers comparing SLT and PLT up to January 2022. (3) Results: 25 studies describing 11,275 patients met the inclusion criteria. The meta-analysis revealed no statistical difference in intraoperative blood loss, overall vascular complications, retransplantation rate, and hospital stay in the SLT group compared with the PLT group. However, the SLT group showed a slightly significant lower 5-year OS rate and 5-year disease-free survival rate. (4) Conclusion: meta-analysis advocates the relative safety and feasibility of both Salvage LT and Primary LT strategies. Specifically, SLT seems to have comparable surgical outcomes but slightly poorer long-term survival than PLT.
Centimeter-Scale Curing Depths in Laser-Assisted 3D Printing of Photopolymers Enabled by Er3+ Upconversion and Green Light-Absorbing Photosensitizer

Photopolymer resins used in stereolithographic 3D printing are limited to penetration depths of less than 1 mm. Our approach explores the use of near-infrared (NIR) to visible upconversion (UC) emissions from lanthanide-based phosphors to initiate photopolymer crosslinking at a much higher depth. This concept relies on the use of invisibility windows and non-linear optical effects to achieve selective crosslinking in photopolymers. SLA resin formulation capable of absorbing light in the visible region (420–550 nm) was developed, in order to take advantage of efficient green-UC of Er3+/Yb3+ doped phosphor. NIR-green light UC shows versatility in enhancing curing depths in laser patterning. For instance, a structure with a curing depth of 11 ± 0.2 mm, cured width of 496 ± 5 µm and aspect ratios of over 22.2:1 in a single pass via NIR-green light UC. The penetration depth of the reported formulation approached 39 mm. Therefore, this technique would allow curing depths of up to 4 cm. Moreover, it was also demonstrated that this technique can initiate cross-linking directly at the focal point. This shows the potential of NIR-assisted UC as a low-cost method for direct laser writing in volume and 3D printing.
Some Remarks on a Method of Maximum-likelihood Estimation Proposed by Richards

SUMMARY Richards (1961) suggested a method of maximum-likelihood estimation for the case of several parameters. Some gaps in the general theory given by him in support of the method are pointed out. Though the method is justifiable, the justification is shown to depend on the correct use of the implicit function theorem and is not connected with the joint asymptotic normality and the asymptotic efficiency of the maximum-likelihood estimates.
DECENTRALIZED OPF HANDLING DISCRETE VARIABLES IN INTERCONNECTED MULTI-AREA POWER SYSTEMS

This paper presents a method for the decentralized solution of the Optimal Power Flow (OPF) problem of interconnected power systems, with both continuous and discrete variables. The method decomposes the central OPF problem of a multi-area system into independent OPF sub-problems, one for each area. The mathematical decomposition method is based on the decoupling of the first-order KKT (Karush-Kuhn-Tucker) conditions of the original system-wide OPF problem. The solutions of the OPF sub-problems of the different areas are coordinated through a pricing mechanism until they converge to the system-wide OPF solution. A discretization logic is applied to the solution of the OPF problem with continuous variables, for the handling of the discrete variables. The method requires no parameter tuning for reaching conve rgence or faster convergence. Results from the application of the method to several IEEE test systems are presented. I
Cebpb Regulates Skeletal Stem Cell Osteogenic Differentiation and Fracture Healing via the WNT/β-Catenin Pathway

Fracture is the most common traumatic organ injury, and fracture nonunion is a critical clinical challenge. The research on the mechanisms of skeletal stem cell (SSC) differentiation and fracture healing may help develop new treatment strategies and improve the prognosis of patients at high risk of nonunion. Bioinformatic analysis of scRNA-seq data of mouse SSCs and mouse osteoprogenitors was applied to discover major transcription factors for the regulation of SSC differentiation. FACS was used to isolate SSCs prospectively. The expression of Cebpb, osteogenesis-related genes (Runx2, Sp7, and Bglap2), and markers for Notch, Hedgehog, MAPK, BMP2/SMAD, and WNT/β-catenin signaling pathways (Hes1, Gli1, p-Erk1/2, p-Smad1/5/9, and β-catenin) were detected in SSCs with qPCR or western blot, respectively. Alkaline phosphatase assay and alizarin red S staining were used to illustrate the osteogenic differentiation ability of SSCs in vitro. A WNT inhibitor, IWR-1, was further used to explore the mechanism of WNT signaling in the differentiation of SSCs. Micro-CT, mechanical testing, and immunohistochemistry of osteogenic and chondrogenic proteins (Sp7 and Col2α1) were used to demonstrate the capacity of Cebpb knockdown in promoting fracture healing in a monocortical defect model. We found that Cebpb was the crucial transcription factor regulating SSC differentiation. Inhibiting Cebpb in SSCs enhanced the expression of active β-catenin to promote the expression of WNT target genes, thus facilitating the osteogenic differentiation of SSCs. Bone mass, mechanical properties, and osteogenic protein expression were also increased in the Cebpb inhibition group compared to the group without Cebpb inhibition. Collectively, our results proved that Cebpb knockdown promotes SSC osteogenic differentiation and fracture healing via the WNT/β-catenin signaling pathway.
The Best of Both Worlds: Exploring the Experiences of Alternatively Certified Agriculture Teachers

Alternative certification is often seen by policymakers and local school districts as the answer to the teacher shortage problem. Yet, little is known about the experiences of alternatively certified teachers in agricultural education. This case study sought to explore the experiences of alternatively certified agriculture teachers through a composite case study grounded in Dewey’s conception of experience. The participants for this study included four early career alternatively certified teachers in Wisconsin. Our findings illuminate insights regarding the fortuitous nature of alternatively certified teachers entering the classroom, the importance of their prior experiences, their passion for the content, the hurdles of becoming certified, and the significance of support. This is the first study of its kind in agricultural education to examine the first-hand accounts of alternatively certified teachers in an attempt to understand the benefits and challenges and the stepping stones leading to alternative certification. How are alternatively certified teachers becoming certified? What personal and professional experiences are they utilizing in the agricultural education classroom? It is critical that we do not disregard these teachers or their valuable experience, but instead seek to understand their background and certification process to ensure continued school-based agricultural education success.
The Effect of Chronotype on Addictive Eating Behavior and BMI among University Students: A Cross-Sectional Study

The aim of this study was to examine the effect of chronotype on addictive eating behavior and BMI in university students. A questionnaire on their sociodemographic characteristics and eating habits, as well as the three-factor eating questionnaire (TFEQ) and the morningness-eveningness questionnaire (MEQ), were given to students at Üsküdar University. Data were analyzed with IBM SPSS 26.0. This study included a total of 850 students, 46.8% of whom were male and 53.2% were female. The mean BMI was 22.94 ± 3.30 kg/m2. As the BMI values of students increased, there was a decrease of 25.6% in the TFEQ scores, regardless of gender (s = −0.256; p < 0.001), and an increase of 10.6% in their chronotype scores, meaning that the students tended to be morning types (s = 0.106; p < 0.01). A significantly negative, very weak correlation was found between the students’ TFEQ and MEQ scores (s = −0.123; p < 0.001). The students’ BMI (p < 0.01) and TFEQ scores (p < 0.001) were affected by their MEQ scores in a statistically significant way. It was found that each 1-point increase in the MEQ score led to a 4.0% increase in the BMI score and a 15.8% decrease in the TFEQ score. It was observed that the morning-type students tended to be obese in terms of their BMI, and these students demonstrated fewer addictive eating behaviors.
TVA's dose algorithm for Panasonic type 802 TLDs

The TVA algorithm for interpreting readings from Panasonic type 802 multi-element TLDs uses a calculational method similar to that for unfolding neutron spectra. A response matrix is constructed for the four elements in the Panasonic TLD based on tests performed in a variety of single-component radiation fields. The matrix is then used to unfold the responses of the elements when the dosimeter is exposed to mixed radiation fields. In this paper the response metric and the calculational method are described in detail, and test results are presented that verify the algorithm's effectiveness.
Ejected nebulae as probe of Wolf-Rayet Lyman-continua

We present the rst results from our analysis of WR model atmospheres in the UV domain. Speciically, we test the model atmospheres derived for WR124 and WR18 from the Hillier & Miller (1998) and Schmutz (1997) codes through photoionization modelling of the associated nebula or HII region. Both codes assume non-LTE and line blan-keting, while photon loss is implemented only in Schmutz (1997) code. The comparison of the predicted nebular properties with observations indicates that: i) line blanketing is essential in reconciling the stellar and nebular properties of WR stars; ii) in the case of WR18 the inclusion of photon loss produces a slightly more ionized nebula than observed.