Miracle-dz/UniFAR-FTUs huggingface dataset:
Stemness-Suppressive Effect of Bibenzyl from Dendrobium ellipsophyllum in Human Lung Cancer Stem-Like Cells

Cancer stem-like cells (CSCs) are key mediators driving tumor initiation, metastasis, therapeutic failure, and subsequent cancer relapse. Thus, targeting CSCs has recently emerged as a potential strategy to improve chemotherapy. In this study, the anticancer activity and stemness-regulating capacity of 4,5,4′-trihydroxy-3,3′-dimethoxybibenzyl (TDB), a bibenzyl extracted from Dendrobium ellipsophyllum, are revealed in CSCs of various human lung cancer cells. Culture with TDB (5–10 μM) strongly abolished tumor-initiating cells in lung cancer H460, H23, and A549 cells in both anchorage-dependent and anchorage-independent colony formation assays. Through the 3D single-spheroid formation model, attenuation of self-renewal capacity was observed in CSC-enriched populations treated with 1–10 μM TDB for 7 days. Flow cytometry analysis confirmed the attenuation of %cell overexpressing CD133, a CSC biomarker, in TDB-treated lung cancer spheroids. TDB at 5–10 μM remarkably suppressed regulatory signals of p-Akt/Akt, p-GSK3β/GSK3β, and β-catenin corresponding to the downregulated mRNA level of stemness transcription factors including Nanog, Oct4, and Sox2. Moreover, the antiapoptosis Bcl-2 and Mcl-1 proteins, which are downstream molecules of Akt signaling, were evidently decreased in CSC-enriched spheroids after culture with TDB (1–10 μM) for 24 h. Interestingly, the diminution of Akt expression by specific siAkt effectively reversed suppressive activity of TDB targeting on the CSC phenotype in human lung cancer cells. These findings provide promising evidence of the inhibitory effect of TDB against lung CSCs via suppression of Akt/GSK3β/β-catenin cascade and related proteins, which would facilitate the development of this bibenzyl natural compound as a novel CSC-targeted therapeutic approach for lung cancer treatment.
The complete mitochondrial genome of Coriandrum sativum

Abstract Using Oxford Nanopore and Illumina sequencing technologies, we reported the first complete mitochondrial genome of the important medicinal and edible plant Coriandrum sativum. The complete mitogenome was assembled into two circular-mapping forms of 82,926 bp (cir1) and 224,590 bp (cir2), respectively. There were 28 genes identified in the cir1 mitogenome, which included 14 protein-coding genes, 2 rRNA genes and 12 tRNA genes. There were 62 genes identified in the cir2 mitogenome, which included 41 protein-coding genes, 5 rRNA genes and 16 tRNA genes. Phylogenetic analysis showed that Coriandrum sativum was most closely related to Daucus carota.
The complete chloroplast genome of the subtropical species Camellia japonica ‘Huaheling’

Abstract Camellia japonica ‘Huaheling’ is a rare subtropical Camellia species in China with high ornamental and medicinal value. The complete chloroplast genome of C. japonica ‘Huaheling’ is a 157,001-bp circular DNA molecule containing a large single-copy region (LSC, 86,704 bp), a small single-copy region (SSC, 18,393 bp), and two inverted repeat sequences (IR). Of the 131 genes identified, 86 are protein-coding genes, 8 are rRNA genes, and 37 are tRNA genes. A total of 54 simple sequence repeats (SSRs) were identified in the chloroplast genome. The phylogenetic analysis showed that C. japonica ‘Huaheling’ is clustered with C. japonica. This work provides valuable information for future study of the evolution and genetic diversity of C. japonica ‘Huaheling. ’
Plasma Metabolites in Early Sepsis Identify Distinct Clusters Defined by Plasma Lipids

Supplemental Digital Content is available in the text. OBJECTIVES: Unbiased global metabolomic profiling has not been used to identify distinct subclasses in patients with early sepsis and sepsis-associated acute respiratory distress syndrome. In this study, we examined whether the plasma metabolome reflects systemic illness in early sepsis and in acute respiratory distress syndrome. DESIGN: Plasma metabolites were measured in subjects with early sepsis. SETTING: Patients were admitted from the emergency department to the ICU in a plasma sample collected within 24 hours of ICU admission. Metabolic profiling of 970 metabolites was performed by Metabolon (Durham, NC). Hierarchical clustering and partial least squares discriminant clustering were used to identify distinct clusters among patients with early sepsis and sepsis-associated acute respiratory distress syndrome. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Among critically ill patients with early sepsis (n = 197), three metabolically distinct subgroups were identified, with metabolic subtype driven by plasma lipids. Group 1, with 45 subjects (23% of cohort), had increased 60-day mortality (odds ratio, 2; 95% CI, 0.99–4.0; p = 0.04 for group 1 vs all others). This group also had higher rates of vasopressor-dependent shock, acute kidney injury, and met Berlin acute respiratory distress syndrome criteria more often (all p < 0.05). Conversely, metabolic group 3, with 76 subjects (39% of cohort), had the lowest risk of 60-day mortality (odds ratio, 0.44; 95% CI, 0.22–0.86; p = 0.01) and lower rates of organ dysfunction as reflected in a lower Simplified Acute Physiology Score II (p < 0.001). In contrast, global metabolomic profiling did not separate patient with early sepsis with moderate-to-severe acute respiratory distress syndrome (n = 78) from those with sepsis without acute respiratory distress syndrome (n = 75). CONCLUSIONS: Plasma metabolomic profiling in patients with early sepsis identified three metabolically distinct groups that were characterized by different plasma lipid profiles, distinct clinical phenotypes, and 60-day mortality. Plasma metabolites did not distinguish patients with early sepsis who developed acute respiratory distress syndrome from those who did not.
Enormous Single-Session Septotomy for the Treatment of Late Leak Postsleeve Gastrectomy

A 52-year-old man with a history of laparoscopic sleeve gastrectomy presented 8 weeks postoperatively with acute symptoms of abdominal pain, nausea, vomiting, and inability to tolerate oral intake. A previous attempt of primary closure with endoscopic suturing using theApolloOverStitchwas unsuccessful (Figure 1). Aweek later, a partially covered self-expandablemetal stent 18mm 3 15 cm was placed to create a watertight seal across the leak site. However, this also failed to close the leak, and the decision was made tomove from a strategy of closure to keeping the fistula open because this was now classified as a late leak present at more than 12 weeks postsleeve gastrectomy. Treatment options included double pigtail, endoscopic septotomy, and endoscopic vacuum therapy.
Current pool of ultimate collection of mitochondrial DNA from remnants of Kalash

Abstract The mitochondrial DNA (mtDNA) complete control region coverage of 111 individuals from Kalash population of Pakistan has been presented for forensic applications and to infer their genetic parameters. We detected in total 14 different haplotypes with only five unique and nine shared by more than one individual. This population has come up with quite lower haplotype diversity (0.8393) and very higher random match probability (0.1682), and ultimately lower power of discrimination (0.832). Additionally, haplogroup distribution reveals the genetic ancestry of Kalash, mainly from West Eurasia (98.8%) and very little from South Asia (0.9%). Neither African lineages nor East Asian genetic segments were detected among these Kalash. This study will contribute to the database development for forensic applications as well as to track the evolutionary highlights of this ethnic group.
Does market segmentation hinder interregional CO2 flow in China? — Evidence from China’s interprovincial MRIO table

China is the key player in the globalization era and is eliminating its intra-national trade barrier. This process will affect interprovincial CO2 flows. This study recalculates interprovincial CO2 flows in China by using the latest MRIO table and applies a gravity model to assess how market segmentation affects interprovincial CO2 flows. Results show that the total volume of interprovincial embodied CO2 flow did not increase excessively from 2007 to 2012, but the pattern of embodied CO2 flow had changed a lot. Market segmentation significantly decreased the interprovincial embodied CO2 flows in China and within its sub-regions. At interregional level, market segmentation’s negative effect was significant between Central and Western China. Other variables such as geographical distance showed a significant negative impact on interprovincial embodied CO2 flow in China. On the basis of our results, we raise some relevant policies to deal with the environmental inequality caused by the decrease in market segmentation.
Efficacy and Safety of Paromomycin for Visceral Leishmaniasis: A Systematic Review

Visceral leishmaniasis, also known as kala-azar is one of the most commonly neglected tropical diseases affecting a large number of rural and resource-limited people in South Asia, Africa, and South America. Paromomycin, an aminoglycoside drug, is frequently used for the treatment of visceral leishmaniasis. Despite limited therapies for visceral leishmaniasis and emerging drug resistance, a proper review on the action of paromomycin for kala-azar is lacking. This systematic review aims to look for the efficacy and safety aspects of paromomycin for the treatment of visceral leishmaniasis.
Relationships among feed efficiency traits across production segments and production cycles in cattle

Abstract Understanding the relationships between feed efficiency traits measured in different stages of production is necessary to improve feed efficiency across the beef value chain. The objective of this study was to evaluate relationships among feed efficiency traits measured as growing heifers and breeding females and in their progeny in three full production cycles, and relationships of dam residual feed intake (RFI) with lifetime and lifecycle cow efficiency traits. Data were collected on 160 mixed-breed heifers from 240 d of age to weaning of their third progeny, and postweaning performance of progeny until harvest in experiments initiated in 1953, 1954, 1959, 1964, 1969, and 1974. Individual feed offered was recorded daily, and feed refusals measured every 28 d. Milk yield was measured at 14-d intervals throughout lactation by machine or hand milking. Females and progeny were weighed at 28-d intervals and progeny were harvested at a constant endpoint of live grade or age depending upon the experiment. Feed efficiency traits of RFI and residual BW gain (RG) were computed as the residual from linear regression for developing heifers, dams (RFI and residual energy-corrected milk [RECM]), and postweaning progeny. Feed:gain ratio (FCR) was computed for developing heifers and postweaning progeny, and feed:milk energy ratio (FME) was computed for dams. Various measures of cow efficiency were calculated on either a life cycle or lifetime basis using ratios of progeny and dam weight outputs to progeny and dam feed inputs. Pearson correlations were computed among traits adjusted for a random year-breed-diet group effect. Heifer RFI (0.74) and RG (−0.32) were correlated (P ≤ 0.05) with dam RFI in parity 1 only, but were not correlated (P > 0.05) with dam RECM in any parity. Heifer RFI was correlated (P ≤ 0.05) with progeny RFI (0.17) in parity 3 only. Heifer FCR was not correlated with dam FME or progeny FCR in any parity. Dam RFI was weakly correlated (r = 0.25 to 0.36; P ≤ 0.05) among parities, whereas dam FME and RECM were strongly correlated (r = 0.49 to 0.72; P ≤ 0.05) among parities. Dam RFI in parity 1 and 2 was weakly correlated (r = −0.20 to −0.33; P ≤ 0.05) with cow efficiency ratios that included dam weight as an output, whereas dam RFI in parity 3 was not correlated with any cow efficiency ratio. In conclusion, feed efficiency traits were poorly correlated across production segments, but moderately repeatable across production cycles.
Insight for Immunotherapy of HCMV Infection

Human cytomegalovirus (HCMV), a ubiquitous in humans, has a high prevalence rate. Young people are susceptible to HCMV infection in developing countries, while older individuals are more susceptible in developed countries. Most patients have no obvious symptoms from the primary infection. Studies have indicated that the virus has gradually adapted to the host immune system. Therefore, the control of HCMV infection requires strong immune modulation. With the recent advances in immunotherapy, its application to HCMV infections is receiving increasing attention. Here, we discuss the immune response to HCMV infection, the immune escape mechanism, and the different roles that HCMV plays in various types of immunotherapy, including vaccines, adoptive cell therapy, checkpoint blockade therapy, and targeted antibodies.
Clinical Randomized Controlled Study of Acupuncture Treatment on Children with Autism Spectrum Disorder (ASD): A Systematic Review and Meta-Analysis

This study aimed to summarize the effectiveness and safety of acupuncture in the treatment of autism spectrum disorder (ASD) through literature analysis and evaluation. All studies were retrieved from various databases as follows: English databases, such as PubMed, Cochrane Library, Ovid, and Web of Science, and Chinese databases, such as China National Knowledge Infrastructure (CNKI), WanFang Data (WF), and Technology Periodical Database (VIP). The Cochrane Collaboration's Bias Risk Assessment Scale was used to assess the studies' risk of bias. The effects of acupuncture treatment for ASD were determined using the following indicators: childhood autism rating scale (CARS), autism behavior check list (ABC), Reynell developmental language scale (RDLS), and functional independence measure of children (WeeFIM). The risk map of bias of these studies' quality and the meta-analysis results of the indicators was prepared with RevMan 5.2 software. Finally, 16 studies were included, five of which were in English and 11 were in Chinese. The 16 studies included 1332 patients. The CARS results for subgroup analysis were as follows: acupuncture subgroup (MD = −2.65, 95% CI (−3.22, −2.07)) and acupuncture plus massage subgroup (MD = −10.35, 95% CI (−11.34, −9.36)). The ABC results were as follows: (MD = −6.70, 95% CI (−9.10, −4.29)). The analysis results of sensory, relating, language, body and object use, and social/self-help in the subitems of ABC were as follows: sensory (MD = −2.67, 95% CI (−2.90, −2.44)), relating (MD = −3.28, 95% CI (−3.55, −3.02)), language (MD = −2.45, 95% CI (−2.73, −2.16)), body and object use (MD = −1.19, 95% CI (−1.38, −1.00)), and social/self-help (MD = −2.09, 95% CI (−2.30, −1.89)). For the analysis results of comprehension and expression ages in the subitems of RDLS, the comprehension age results were as follows: (MD = 0.08, 95% CI (−0.06, 0.22), P = 0.27). Those of expression age were as follows: (MD = 0.15, 95% CI (0.04, 0.26), P=0.009). The WeeFIM results were as follows: (MD = 3.70, 95% CI (2.38, 5.02)). This study suggested that acupuncture could effectively treat ASD. However, acupuncture methods and prescriptions at this stage remain heterogeneous, and acupuncture treatment operations require standardization. Studies using rigorous and standard research designs are needed to draw stronger conclusions about the advantages of using acupuncture to treat children and adolescents with ASD.
Giant Actinomyces brain abscess in an immunocompetent child: A management strategy

Background: Intraparenchymal brain abscess is a collection of microbes caused by inoculation through direct extension or hematogenous spread. Although rare, intraparenchymal abscesses are potentially fatal and can be detected when patients are symptomatic due to local mass effect on adjacent neural tissue. Brain abscess treatment includes medical management with appropriate antibiotics alone or medical management in combination with surgical debridement. Treatment strategies depend on the size and location of disease, as well as the virulence of the microorganism. Similar to medical management strategies, surgical strategies among providers are not uniform, with variation in approaches from complete extirpation of the abscess, including the abscess wall, to minimally invasive stereotactic needle aspiration. In particular, for children, there are no guidelines for therapy. Case Description: We report a case of giant Actinomycosis right frontal brain abscess in an immunocompetent child without risk factors. A review of the literature for the treatment of brain abscess caused very rarely by Actinomyces in children is performed. Conclusion: Successful treatment of brain access depends on organism and location. The even more uncommon giant intraparenchymal abscesses can be managed with minimal access and prolonged antibiosis, especially when slow-growing organisms are identified. Long-term follow-up should be employed to mitigate missed late failures.
Response to the comments about the submitted paper A case study of wind farm effects using two wake parameterizations in WRF ( V 3 . 7 . 1 ) in the presence of low level jets

We thank the reviewers for his constructive comments. We have addressed each and every one of them and modified the paper accordingly. Our detailed answers follow. Please note that reviewers’ comments are in italics while our answers are not. Additions to the original manuscript are indicated in blue. There are three parts in this file: Answers to reviewer 1, answers to reviewer 2 and attachment README.md. Please read the README.md-file at the end this file for all the updates.
Ju l 2 02 1 Counting extensions revisited

We consider rooted subgraphs in random graphs, i.e., extension counts such as (i) the number of triangles containing a given vertex or (ii) the number of paths of length three connecting two given vertices. In 1989, Spencer gave sufficient conditions for the event that, with high probability, these extension counts are asymptotically equal for all choices of the root vertices. For the important strictly balanced case, Spencer also raised the fundamental question as to whether these conditions are necessary. We answer this question by a careful second moment argument, and discuss some intriguing problems that remain open.
Momentum-based Gradient Methods in Multi-Objective Recommendation

Multi-objective gradient methods are becoming the standard for solving multi-objective problems. Among others, they show promising results in developing multi-objective recommender systems with both correlated and conflicting objectives. Classic multi-gradient~descent usually relies on the combination of the gradients, not including the computation of first and second moments of the gradients. This leads to a brittle behavior and misses important areas in the solution space. In this work, we create a multi-objective model-agnostic Adamize method that leverages the benefits of the Adam optimizer in single-objective problems. This corrects and stabilizes~the~gradients of every objective before calculating a common gradient descent vector that optimizes all the objectives simultaneously. We evaluate the benefits of Multi-objective Adamize on two multi-objective recommender systems and for three different objective combinations, both correlated or conflicting. We report significant improvements, measured with three different Pareto front metrics: hypervolume, coverage, and spacing. Finally, we show that the \textit{Adamized} Pareto front strictly dominates the previous one on multiple objective pairs.
Strategies for convex potential games and an application to decision-theoretic online learning

The backwards induction method due to Bellman [1] is a popular approach to solving problems in optimiztion, optimal control, and many other areas of applied math. In this paper we analyze the backwords induction approach, under min/max conditions. We show that if the value function is has strictly positive derivatives of order 1-4 then the optimal strategy for the adversary is Brownian motion. Using that fact we analyze diﬀerent potential functions and show that the Normal-Hedge potential is optimal.
A Learning Approach to Robot-Agnostic Force-Guided High Precision Assembly

In this work we propose a learning approach to high-precision robotic assembly problems. We focus on the contact-rich phase, where the assembly pieces are in close contact with each other. Unlike many learning-based approaches that heavily rely on vision or spatial tracking, our approach takes force/torque in task space as the only observation. Our training environment is robotless, as the end-effector is not attached to any specific robot. Trained policies can then be applied to different robotic arms without re-training. This approach can greatly reduce complexity to perform contact-rich robotic assembly in the real world, especially in unstructured settings such as in architectural construction. To achieve it, we have developed a new distributed RL agent, named Recurrent Distributed DDPG (RD2), which extends Ape-X DDPG[1] with recurrency and makes two structural improvements on prioritized experience replay[2]. Our results show that RD2 is able to solve two fundamental high-precision assembly tasks, lap-joint and peg-in-hole, and outperforms two state-of-the-art algorithms, Ape-X DDPG and PPO with LSTM. We have successfully evaluated our robot-agnostic policies on three robotic arms, Kuka KR60, Franka Panda, and UR10, in simulation. The video presenting our experiments is available at https://sites.google. com/view/rd2-rl
Fast model‐based clustering of partial records

Partially recorded data are frequently encountered in many applications and usually clustered by first removing incomplete cases or features with missing values, or by imputing missing values, followed by application of a clustering algorithm to the resulting altered dataset. Here, we develop clustering methodology through a model‐based approach using the marginal density for the observed values, assuming a finite mixture model of multivariate t distributions. We compare our approximate algorithm to the corresponding full expectation–maximization (EM) approach that considers missing values in the incomplete dataset and makes a missing at random (MAR) assumption, as well as case deletion and imputation methods. Since only the observed values are utilized, our approach is computationally more efficient than imputation or full EM. Simulation studies demonstrate that our approach has favourable recovery of the true cluster partition compared to case deletion and imputation under various missingness mechanisms and is at least competitive with the full EM approach, even when MAR assumptions are violated. Our methodology is demonstrated on a problem of clustering gamma‐ray bursts and is implemented at https://github.com/emilygoren/MixtClust.
Cluster Algebras and Scattering Diagrams, Part II. Cluster Patterns and Scattering Diagrams

We review some important results by Gross, Hacking, Keel, and Kontsevich on cluster algebra theory, namely, the column sign-coherence of $C$-matrices and the Laurent positivity, both of which were conjectured by Fomin and Zelevinsky. We digest and reconstruct the proofs of these conjectures by Gross et al. still based on their scattering diagram method, however, without relying on toric geometry. At the same time, we also give a detailed account of the correspondence between the notions of cluster patterns and scattering diagrams. Most of the results in this text are found in or translated from the known results in the literature. However, the approach, the construction of logic and proofs, and the overall presentation are new. Also, as an application of the results and the techniques in the text, we show that there is a one-to-one correspondence between $g$-vectors and cluster variables in cluster patterns with arbitrary coefficients.