Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effect of GPRC5a on the Proliferation, Migration Ability, Chemotherapy Resistance, and Phosphorylation of GSK-3β in Pancreatic Cancer

Pancreatic cancer (PaCa) is the fourth leading cause of cancer-related death, and personalized targeted cancer therapy is becoming a promising treatment strategy for PaCa. The central approach of targeted therapy is to find a targetable key and an effective targeting method. In this study, the importance of GPRC5a (the G-protein-coupled receptor family C, member 5, group A) was identified using data mining methods based on published datasets. After analysis of the basic expression of GPRC5a in normal pancreas tissue and various PaCa cell lines, gene editing of GPRC5a in the human PaCa cell line MIA PaCa-2 and the mouse PaCa cell line TB32047 was performed using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins 9) to investigate the influence of GPRC5a on the proliferation and migration of PaCa cells as well as its effects on chemotherapy drug resistance. The results showed that GPRC5a was upregulated in PaCa tissues and various PaCa cell lines. Knockout of GPRC5a reduced the proliferation and migration ability of PaCa cell lines and suppressed the chemotherapy drug resistance of gemcitabine, oxaliplatin, and fluorouracil in PaCa cells. The phosphorylation of GSK-3β (Glycogen synthase kinase-3β) was found to be upregulated in the MIA PaCa-2 and TB32047 cells after GPRC5a knockout. In conclusion, GPRC5a was upregulated in PaCa leading to an enhanced drug resistance in PaCa cells. These results provide for the first time a theoretical basis for the development of an improved PaCa targeted therapy.
Individual Redemption through Universal Design; or, How IEP Meetings Have Infused My Pedagogy with an Ethic of Care(taking)

I address how participating as a parent in the Individualized Education Program (IEP) process has helped to transform my own approach to teaching by reinforcing how important it is to endorse a pedagogy that recognizes and values the individuality of my students. Universal Design for Instruction (UDI) has served as the best model I have found to help me move closer to my ideal classroom, and the course that most reflects this ideal classroom is my upper-level Disability and Literature course. The course’s greatest strength lies in the extent to which its format and delivery are inextricably tied to its subject matter through an ethic of care(taking), especially through the incorporation of a number of UDI features into this course. What is more, while the traditional class meetings remain a privileged site of collaborative engagement and learning, the course blog is an equally crucial component to such collaboration, as the students create nearly all of its content. Indeed, the blog space serves not only as a place for students to record their responses to the assigned readings and in-class discussions but also as a student-driven supplement to the instructor-supplied focus points, a supplement that truly expands the range of possibilities implicitly represented in my choice of readings. I now understand such a pedagogical orientation not simply as a generic model for “good teaching” but as a reflection of a disability-inflected pedagogy of care.
Tüketicilerin Online Alışveriş Davranışını Etkileyen Faktörler

Her gecen gun cesitli online faaliyetlerle ilgilenen kisi sayisi artmaktadir. Dolayisiyla, online tuketici davranisi, pazarlama bilimindeki en onemli arastirma alanlarindan biri kabul edilmektedir. Ancak, sosyo-teknik ve karmasik bir konu oldugundan ve bircok faktoru icerdiginden, sinirli bir bilginin varligi dikkat cekmektedir. Bu arastirmanin amaci, bazi onemli kuramlara dayali olarak online tuketici davranisini etkileyen faktorleri incelemektir. Arastirmada, Aksaray’daki tuketicilerden amaca yonelik olusturulmus bir anketle veri toplanmistir. Veriler Standart Coklu Regresyon ile analiz edilmistir. Bulgular, hem teknoloji yonlu hem de tuketici yonlu degiskenlerin online tuketici davranisi acisindan onemli oldugunu ortaya koymustur
Praktijkgericht Kwalitatief Onderzoek

1. Methodische aspecten van kwalitatief onderzoek 2. zelfstandigheid en ouderenzorg: een evaluatieonderzoek in een wooncentrum voor ouderen 3. De casestudy als strategie in het toegepast onderzoek 4. Onderzoek welzijnswerk 5. Formatief evaluatieonderzoek in een veranderende beleidscontext 6. Rehabilitatie van psychiatrische patienten 7. Handelingsonderzoek: hoe doe je dat? 8. Inductieve, analoge en communicatieve generalisatie 9. Praktijkgericht kwalitatief onderzoek: problemen en perspectieven
Realistic Mathematics Education in the Netherlands under the Concept of Secondary School Mathematics Curriculum Goals Thinking

Netherlands Reality Mathematics Education Research has been made achievements for half a century.Our curriculum reform and the background are very similar to the Netherlands.It is great significance to study its educational philosophy for China's reform of mathematics curriculum.There are enlightenments of the reality mathematics education:(1) The content of textbooks should be more life;(2) Evaluation should be the process of caring and understanding;(3) Teachers should change the concept.
Guidelines for Designing Web-Based Instruction in Higher Education.

To develop practical Web-based instruction (WBI) design guidelines for faculty and instructional support staff, the author analyzed three different WBI design cases based on interviews with three experts. On the surface, the WBI design processes that each expert used look more alike than different, entailing similar major steps. The underlying principles and knowledge that guided each expert through the design processes, however, are unique in that each expert constructed her own set of heuristics to accommodate the myriad of contextual factors that arose in the work setting. After discussing the implications of findings, the author presents a six-phase set of WBI design guidelines.
High order in time schemes for hyperbolic conservation laws by an approximate Cauchy-Kovelevskaya procedure

is R-th order accurate if ũ(x, t) = u(x, t)+O(∆tR+1−l). By using the equation, the time derivatives of u can be computed from space derivatives, in turn obtained by finitedifference approximations. For example, for a scalar equation in one dimension one has ut = −f(u)x, utt = (f (u)ux)x for the first two derivatives. In the general case the expressions involved in the time derivatives can become very complex, requiring the use of symbolic calculus software and resulting on computationally expensive algorithms. The main contribution of this work is an alternative version of the previous method [4] that replaces the exact expressions of the time derivatives of the fluxes by approximations of the adequate order, obtained through finite-difference formulas. Hence, the resulting scheme does not require exact flux derivatives and keeps the accuracy order, leading to a much simpler implementation. In cases where the flux derivatives are complex the approximate version obtains better performance than the exact version. The combination of such schemes with known high order spatial reconstructions, provides a high order method in both space and time in a straightforward way.
A study on relationship between conflict management styles and organizational agility: A case study of Ilam state University, Iran

Article history: Received Feb 28, 2013 Received in revised format 19 September 2013 Accepted 23 October 2013 Available online November 24 2013 The aim of present study is to explore the relationship between various styles of conflict management and organizational agility in Ilam state university. The study is quantitative one, having developmental orientation and comparative approach, which applies the strategy of survey research with an aim of exploration and description. It is implemented as single point and utilizes questionnaire as data collecting tool. The population and sample of the study include 425 and 205 individuals, respectively. The sample size is determined through Cochran’s formula and random sampling method has been used for sampling. In order to perform the data analysis, descriptive and inferential statistics as well as Spearman and Kendall test in SPSS were utilized. The results of the study reveal that there was a positive and significant relationship among four styles of conflict management (avoiding, forcing, compromising, and yielding), however there was no significant relationship between integrating conflict management and organizational agility. © 2014 Growing Science Ltd. All rights reserved.
Seed cryopreservation of Passiflora species1

The aim of this study was to evaluate the cryopreservation of seeds of Passiflora species, using different cryoprotectants. The completely randomized design was used with four treatments (T1 DMSO at 7%; T2 0.3 M sucrose; T3 storage in NL2 without cryoprotectants; T4 control) and five replications of 50 seeds. The seeds were stored for 120 hours in NL2 (-196 °C), and thawing was conducted in a water bath (37 °C) for 20 minutes, proceeding with the germination and vigor tests. The data were submitted to ANOVA and Tukey’s test (5% of probability) using the SISVAR program. In P. mucronata, P. suberosa and P. edulis seeds, the responses obtained were favorable to cryopreservation without the use of cryoprotectants in regards to P. micropetala, there was a considerable loss of germination potential after storage in NL2. Given these circunstances, cryopreservation can be used for long-term conservation of Passiflora mucronata, P. suberous and P. edulis, without the use of cryoprotectants.
Expressive Motion in Mobile Robots

This work addresses motion expressiveness of a mobile robot by performing a case study to analyze its effects on humans’ reactions. To achieve this, a controller enabling the robot to perform expressive motion was developed and experiments conducted. The experiments took place in two distinct places in a laboratory, which is a controlled environment, and in a hospital, which is a real environment. At the laboratory, Likert scale questionnaires were employed in order to discern the opinions of the participants on the subject. At the hospital, the experiment was recorded in video streaming and carried as a ”Wizard of Oz”. Afterwards, the participants behaviours’ were statistically analysed to get the correlation between expressiveness and the expected human reactions. Given that several correlation measures were found, we believe to have proven that expressive motion does play a role on the impact of a social robot, making it more likely to be attractive and pleasing to the people around it.
Sea level trend and variability in the Singapore Strait

Abstract. Sea level in the Singapore Strait (SS) exhibits response to various scale phenomena, from local to global. Longest tide gauge records in SS are analysed to derive local sea level trend and annual, inter-annual and multi-decadal sea level variability, which then are attributed to regional and global phenomena. Annual data gaps are reconstructed using functions correlating sea level variability with ENSO. At annual scale, sea level anomalies in SS are (quasi-periodic) monsoon-driven, of the order of p20 cm, the highest during northeast monsoon and the lowest during southwest monsoon. Interannual regional sea level drops are associated with El Nino events, while the rises are correlated with La Nina episodes; both variations are in the range of p5 cm. At multi-decadal scale, annual measured sea levels in SS are varying with global mean sea level, rising at the rate 1.2–1.7 mm yr−1 for 1975–2009, 1.8–2.3 mm yr−1 for 1984–2009 and 1.9–4.6 mm yr−1 for 1993–2009. When SS rates are compared with the global trends (2.0, 2.4 and 2.8 mm yr−1, respectively) derived from tide gauge measurements for the same periods, they are smaller in the earlier era and considerably larger in the recent one. Taking into account the first estimate of land subsidence rate, 1–1.5 mm yr−1 in Singapore, the recent trend of absolute sea level rise in SS follows regional tendency.
Fabrication and Characterization of Electrospun Pristine and Fluorescent Composite Poly (acrylic acid) Ultra-Fine Fibers

Electrospinning is a facile nanofabrication technique that produces fibrous assemblies of ultra-fine fibers, 20–1000 nm in diameter, from a charged droplet of spinning solution. Optimization of the fiber diameter of a specific material system is dependent on the solution and process variables. The electrospinning parameters for poly (acrylic acid) (PAA), a synthetic polyelectrolyte, were systematically investigated and consistent nanofiber diameters with uniform morphology were achieved. The optimization matrix included several solvent systems including ethanol, aqueous NaCl and aqueous NaOH. Optimized spinning parameters were then applied to electrospinning fluorescent fibrous assembles of quantum dot-PAA ultra-fine fiber composites. Ultrafine composite fibers were prepared by electrospinning aqueous solutions of 6wt% PAA loaded with 0.05, 0.10, 0.15 and 0.20%v/v, carboxylic acid functionalized CdSe/ZnS nanoparticles (SNPs). The resulting composite fibers exhibited uniform fiber morphologies with increasing fiber diameters corresponding to increasing SNP loading. Fluorescence micrographs reveal luminescent fibers with evenly distributed fluorophores in the higher loaded samples. Moreover, laser excited fibers manifest SNP intermittency correlated with small clusters and single SNPs suggesting excellent dispersion in the PAA matrix.
Minutiae Based Feature Level Fusion for Multimodal Biometrics

Multimodal biometrics aims to authenticate an individual with the help of one or more biometric traits. This paper has focused on integrating the minutiae features of retina and fingerprint with feature level fusion. Time optimization is done using Clustering. Extracted Minutiae features of both fingerprint and retina are clustered using K-means Clustering and they are Indexed. Clustered and Indexed data stored in a database is compared with the query future and nearest neighbourhood clusters are identified. Decision is made based on the similarity measure. The proposed system provides an authentication with GAR = 99.96%, FAR = 0.4%.
Silicon pool dynamics and biogenic silica export in the Southern Ocean inferred from Si-isotopes

Silicon isotopic signatures ( 30 Si) of water col- umn silicic acid (Si(OH)4) were measured in the Southern Ocean, along a meridional transect from South Africa (Sub- tropical Zone) down to 57 S (northern Weddell Gyre). This provides the first reported data of a summer transect across the whole Antarctic Circumpolar Current (ACC). 30 Si vari- ations are large in the upper 1000 m, reflecting the effect of the silica pump superimposed upon meridional water trans- fer across the ACC: the transport of Antarctic surface waters northward by a net Ekman drift and their convergence and mixing with warmer upper-ocean Si-depleted waters to the north. Using Si isotopic signatures, we determine different mixing interfaces: the Antarctic Surface Water (AASW), the Antarctic Intermediate Water (AAIW), and thermoclines in the low latitude areas. The residual silicic acid concentra- tions of end-members control the 30 Si alteration of the mix- ing products and with the exception of AASW, all mixing in- terfaces have a highly Si-depleted mixed layer end-member. These processes deplete the silicic acid AASW concentra- tion northward, across the different interfaces, without sig- nificantly changing the AASW 30 Si composition. By com- paring our new results with a previous study in the Australian sector we show that during the circumpolar transport of the ACC eastward, the 30 Si composition of the silicic acid pools is getting slightly, but significantly lighter from the Atlantic to the Australian sectors. This results either from the disso- lution of biogenic silica in the deeper layers and/or from an isopycnal mixing with the deep water masses in the different
Importance of Angiogenin and Endothelial Progenitor Cells After Rehabilitation Both in Ischemic Stroke Patients and in a Mouse Model of Cerebral Ischemia

Background: Rehabilitation therapy is the only available treatment for stroke survivors presenting neurological deficits; however, the underlying molecules and mechanisms associated with functional/motor improvement during rehabilitation are poorly understood. Objective: Our aim is to study the modulation of angiogenin and endothelial progenitor cells (EPCs) as repair-associated factors in a cohort of stroke patients and mouse models of rehabilitation after cerebral ischemia. Methods: The clinical study included 18 ischemic strokes admitted to an intensive rehabilitation therapy (IRT) unit, 18 non-ischemic controls and brain samples from three deceased patients. Angiogenin and EPCs were measured in blood obtained before and up to 6 months after IRT together with an extensive evaluation of the motor/functional status. In parallel, C57BL/6 mice underwent middle cerebral artery occlusion, and the pasta matrix reaching-task or treadmill exercises were used as rehabilitation models. Angiogenin RNA expression was measured after 2 or 12 days of treatment together with cell counts from EPCs cultures. Results: Brain angiogenin was identified in both human and mouse tissue, whereas serum levels increased after 1 month of IRT in association with motor/functional improvement. EPC populations were increased after stroke and remained elevated during follow-up after IRT. The mouse model of rehabilitation by the task-specific pasta matrix exercise increased the number of EPCs at 2 days and increased angiogenin expression after 12 days of rehabilitation. Conclusions: Angiogenin and EPCs are modulated by rehabilitation after cerebral ischemia, suggesting that both angiogenin and EPCs could serve as biomarkers of improvement during rehabilitation or future therapeutic targets.
Corticosterone: effects on feather quality and deposition into feathers

The concentration of the glucocorticoid hormone corticosterone (CORT) is increasingly used in ecology and conservation biology as an integrated measure of the historical record of an individual's hypothalamo–pituitary–adrenal (HPA) activity during feather growth. However, where and how CORT is incorporated in feathers is incompletely known. We therefore examined whether CORT is reliably measured with an enzyme immunoassay, where CORT is incorporated in the feather and where it affects feather quality, and whether CORT incorporation is related to plasma CORT levels, feather growth rate and melanin pigmentation. During the regrowth of plucked tail feathers, we injected pigeons with tritium‐labelled CORT, and implanted a CORT‐releasing pellet to increase plasma CORT concentration for about 3 days. In feather segments, we measured labelled CORT (DPM3H) and we quantified CORT with an enzyme immunoassay EIA (CORTEIA) and double‐checked the results with ultra‐high performance liquid chromatography–tandem mass spectrometry (LCMS) (CORTMS). Administered CORT affected feather structure and colour at the very base of the feather (epidermal collar, ramogenic zone) and reduced growth rate. In contrast, incorporation of CORT into the feather happened mainly in the blood quill, as shown with all three methods (DPM3H, CORTEIA and CORTMS). Incorporation of CORT into feathers was only roughly proportional to plasma concentration, proportional to feather growth rate and increased with melanin pigmentation. Measuring CORT in feather is a way to reveal past events of increased stress during feather growth in birds.
The luminosities of backsplash galaxies in constrained simulations of the Local Group

We study the differences and similarities in the luminosities of bound, infalling and the so-called backsplash galaxies of the Milky Way and M31 using a hydrodynamical simulation performed within the Constrained Local UniversE Simulation (CLUES) project. The simulation models the formation of the Local Group within a self-consistent cosmological framework. We find that even though backsplash galaxies passed through the virial radius of their host halo and hence may have lost a (significant) fraction of their mass, their stellar populations are hardly affected. This leaves us with comparable luminosity functions for infalling and backsplash galaxies and hence little hope to decipher their past (and different) formation and evolutionary histories by luminosity measurements alone. Nevertheless, due to the tidal stripping of dark matter we find that the mass-to-light ratios have changed when comparing the various populations against each other: they are highest for the infalling galaxies and lowest for the bound satellites with the backsplash galaxies in between.
odeling shattercane dynamics in herbicide-tolerant grain sorghum ropping systems

Traditional breeding technology is currently being used to develop grain sorghum [Sorghum bicolor (L.) Moench ssp. bicolor] germplasm that will be tolerant to acetolactate synthase (ALS)-inhibiting herbicides. This technology (InzenTM, DuPontTM) has the potential to improve sorghum production by allowing for the postemergence control of traditionally hard-to-control grasses. However, grain sorghum and shattercane [weedy Sorghum species; Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet ex Davidse] can interbreed and introduced traits such as herbicide tolerance could increase the weediness of the weedy relative. Our objective was to develop a simulation model to assess management options to mitigate risks of ALS-resistance evolution in shattercane populations in US sorghum production areas. Assuming a single major gene confers resistance and gene frequencies change according to the HardyWeinberg ratios we constructed a stage-structured (seedbank, plants) matrix model with annual time steps. The model explicitly considered gene flow from Inzen plants to shattercane populations. The management strategies considered in the model were: a) continuous sorghum, b) sorghum followed by (fb) soybeans and c) sorghum fb fallow fb winter wheat, where postemergence ALS-inhibiting herbicides were only used in Inzen years. During sorghum years two options were tested: continuous Inzen and Inzen fb conventional sorghum, for a total of six management strategies. The parameter values used in the model were obtained from our research, the literature, and expert opinion. For each management strategy we ran deterministic and stochastic simulations (with stochastic levels of herbicide efficacy). The time for resistance evolution was predicted to decrease with increased cropping system complexity (more crop diversity than continuous production of Inzen). Evolution of resistance was predicted to occur rapidly if Inzen sorghum is planted continuously because of high selection pressure (ALS-inhibiting herbicide application) and crop-to-weed gene flow. Rotating Inzen with conventional sorghum did not assist with shattercane management. Rotating Inzen with non-sorghum crops where effective herbicide options are available assisted with keeping shattercane density at low levels while postponing resistance evolution to some extent. Crop and herbicide rotation will be key strategies for shattercane management in Inzen