Miracle-dz/UniFAR-FTUs huggingface dataset:
Attachment and Emotion Regulation: A Cross-Cultural Comparative Study of Iranian and Dutch Gay Men

ABSTRACT Negative beliefs and stigmatization of all that is not heterosexual still have an adverse impact on the mental health of the homosexual population in many countries. The purpose of the present study was to comparatively assess how sociocultural differences regarding the level of homosexuality acceptance impact the adult attachment dimensions and emotion regulation strategies among three groups of Iranian and Dutch gay men. A community sample of 124 gay men (40 of Iranians residing in their home country, 41 of Iranians immigrated to the Netherlands and 43 Dutch) participated in the study and completed Revised Adult Attachment Scale (RAAS) and Emotion Regulation Questionnaire (ERQ). MANOVA and follow-up post-hoc Tukey tests were conducted in order to analyze the data. Results demonstrated a noticeable difference in both studied variables, attachment dimensions (close, anxiety, and depend) and emotion regulation strategies (cognitive reappraisal and expressive suppression), among the three groups of participants. As the Iranian (residing in Iran) group showed the greatest levels of anxiety and emotional suppression with the lowest levels of close (convenience of getting intimate to others), depend (trust and depend on others to be availabale when needed) and cognitive reappraisal (ability to alter the emotion caused by an event, before experiencing it by reinterpreting the situation), while the highest levels of depend, close and cognitive reappraisal and the lowest levels of anxiety and emotional suppression were seen in the Dutch group. Finally, Iranian gay immigrants came half way between. This data highlights the role of cultural differences in terms of homosexuality acceptance or stigmatization, in the way gay men exhibit their attachment and manage their emotions either by reappraisal or suppression. Comparative cross-cultural studies are possibly able to open paths to new research on psychological factors of non-heterosexuals in different countries with various cultures and religions.
Study on the Crowd Evacuation Model in High-rise Building Fire

The safe evacuation design of high-rise buildings is an important part of the building design.Studies on the crowd evacuation model in high-rise building fire have been carried out both at home and abroad.This paper analyzed the crowd evacuation model in high-rise building with present examples and proposed effective measures to reduce fire hazards.This has significant instructional meanings to the safe evacuation design.
Cyclin‐dependent kinase 4 expression alters the number of keratinocyte stem cells in the mouse hair follicle

Hair follicles regenerate periodically by spontaneously undergoing cycles of growth, regression, and relative quiescence. During the hair cycle, follicle stem cells residing in a specialized niche remain quiescent, and they are stimulated to proliferate throughout the growth phase of the hair follicle. Although cell cycle regulators play a prominent role during the activation of hair follicle stem cells, the identity and the role of these regulators have not been confirmed. Herein, we reported that stem cells located in the bulge region of the HF (BuSCs) express high levels of cyclin‐dependent kinase 4 (CDK4) through the quiescent phase of the hair cycle. Using gain‐ and loss‐of‐function studies, we have determined that the CDK4 protein level affects the number of BuSCs. Transgenic expression of CDK4 in the bulge region of the hair follicles reduces the number of BuSCs, whereas CDK4 ablation resulted in an increasing number of BuSCs. These results suggest that deregulation of CDK4 protein levels contributes to distorting the self‐renewal/proliferation balance and, in turn, altering the number of BuSCs.
Effect of dexmedetomidine on cardiorespiratory regulation in spontaneously breathing adult rats

Purpose We examined the cardiorespiratory effect of dexmedetomidine, an α2- adrenoceptor/imidazoline 1 (I1) receptor agonist, in spontaneously breathing adult rats. Methods Male rats (226−301 g, n = 49) under isoflurane anesthesia had their tail vein cannulated for drug administration and their tail artery cannulated for analysis of mean arterial pressure (MAP), pulse rate (PR), and arterial blood gases (PaO2, PaCO2, pH). After recovery, one set of rats received normal saline for control recording and was then divided into three experimental groups, two receiving dexmedetomidine (5 or 50 μg·kg−1) and one receiving normal saline (n = 7 per group). Another set of rats was divided into four groups receiving dexmedetomidine (50 μg·kg−1) followed 5 min later by 0.5 or 1 mg∙kg−1 atipamezole (selective α2-adrenoceptor antagonist) or efaroxan (α2-adrenoceptor/I1 receptor antagonist) (n = 6 or 8 per group). Recordings were performed 15 min after normal saline or dexmedetomidine administration. Results Compared with normal saline, dexmedetomidine (5 and 50 μg·kg−1) decreased respiratory frequency (fR, p = 0.04 and < 0.01, respectively), PR (both p < 0.01), and PaO2 (p = 0.04 and < 0.01), and increased tidal volume (both p = 0.049). Dexmedetomidine at 5 μg·kg−1 did not significantly change minute ventilation (V′E) (p = 0.87) or MAP (p = 0.24), whereas dexmedetomidine at 50 μg·kg−1 significantly decreased V′E (p = 0.03) and increased MAP (p < 0.01). Only dexmedetomidine at 50 μg·kg−1 increased PaCO2 (p < 0.01). Dexmedetomidine (5 and 50 μg·kg−1) significantly increased blood glucose (p < 0.01), and dexmedetomidine at 50 μg·kg−1 increased hemoglobin (p = 0.04). Supplemental atipamezole or efaroxan administration similarly prevented the 50 μg·kg−1 dexmedetomidine-related cardiorespiratory changes. Principal conclusion These results suggest that dexmedetomidine-related hypoventilation and hypertension are observed simultaneously and occur predominantly through activation of α2-adrenoceptors, but not I1 receptors, in spontaneously breathing adult rats.
Mitochondrial membrane potential-enriched CHO host: a novel and powerful tool for improving biomanufacturing capability

ABSTRACT With the aim of increasing protein productivity of Chinese hamster ovary (CHO) cells, we sought to generate new CHO hosts with favorable biomanufacturing phenotypes and improved functionality. Here, we present an innovative approach of enriching the CHO host cells with a high mitochondrial membrane potential (MMP). Stable transfectant pools and clonal cell lines expressing difficult-to-express bispecific molecules generated from the MMP-enriched host outperformed the parental host by displaying (1) improved fed-batch productivity; (2) enhanced long-term cell viability of pools; (3) more favorable lactate metabolism; and (4) improved cell cloning efficiency during monoclonal cell line generation. Proteomic analysis together with Western blot validation were used to investigate the underlying mechanisms by which high MMP influenced production performance. The MMP-enriched host exhibited multifaceted protection against mitochondrial dysfunction and endoplasmic reticulum stress. Our findings indicate that the MMP-enriched host achieved an overall “fitter” phenotype that contributes to the significant improvement in biomanufacturing capability.
Data-Driven Deep Supervision for Medical Image Segmentation

Medical image segmentation plays a vital role in disease diagnosis and analysis. However, data-dependent difficulties such as low image contrast, noisy background, and complicated objects of interest render the segmentation problem challenging. These difficulties diminish dense prediction and make it tough for known approaches to explore data-specific attributes for robust feature extraction. In this paper, we study medical image segmentation by focusing on robust data-specific feature extraction to achieve improved dense prediction. We propose a new deep convolutional neural network (CNN), which exploits specific attributes of input datasets to utilize deep supervision for enhanced feature extraction. In particular, we strategically locate and deploy auxiliary supervision, by matching the object perceptive field (OPF) (which we define and compute) with the layer-wise effective receptive fields (LERF) of the network. This helps the model pay close attention to some distinct input data dependent features, which the network might otherwise ‘ignore’ during training. Further, to achieve better target localization and refined dense prediction, we propose the densely decoded networks (DDN), by selectively introducing additional network connections (the ‘crutch’ connections). Using five public datasets (two retinal vessel, melanoma, optic disc/cup, and spleen segmentation) and two in-house datasets (lymph node and fungus segmentation), we verify the effectiveness of our proposed approach in 2D and 3D segmentation.
Four out of ten married women utilized modern contraceptive method in Ethiopia: A Multilevel analysis of the 2019 Ethiopia mini demographic and health survey

Background Modern contraceptive method is a product or medical procedure that interferes with reproduction from acts of sexual intercourse. Globally in 2019, 44% of women of reproductive age were using a modern method of contraception but it was 29% in sub-Saharan Africa. Therefore, the main aim of this analysis was to assess the prevalence of modern contraceptive utilization and associated factors among married women in Ethiopia. Method The current study used the 2019 Ethiopia mini demographic and health survey dataset. Both descriptive and multilevel mixed-effect logistic regression analysis were done using STATA version 14. A p-value of less than 0.05 and an adjusted odds ratio with a 95% confidence interval were used to report statistically significant factors with modern contraceptive utilization. Result The overall modern contraceptive utilization among married women in Ethiopia was 38.7% (95% CI: 37.3% to 40.0%). Among the modern contraceptive methods, injectables were the most widely utilized modern contraceptive method (22.82%) followed by implants (9.65%) and pills (2.71%). Maternal age, educational level, wealth index, number of living children, number of births in the last three years, number of under 5 children in the household, religion, and geographic region were independent predictors of modern contraceptive utilization. Conclusion In the current study only four out of ten married non-pregnant women of reproductive age utilized modern contraceptive methods. Furthermore, the study has identified both individual and community-level factors that can affect the utilization of modern contraceptive methods by married women in the country. Therefore, concerned bodies need to improve access to reproductive health services, empower women through community-based approaches, and minimize region wise discrepancy to optimize the utilization.
Genome-wide Analysis Reflects Novel 5-Hydroxymethylcytosines Implicated in Diabetic Nephropathy

Long-term complications of type 2 diabetes (T2D) are the major causes for T2D-related disability and mortality. Notably, diabetic nephropathy (DN) has become the most frequent cause of end-stage renal disease (ESRD) in most countries. Understanding epigenetic contributors to DN can provide novel insights into this complex disorder and lay the foundation for more effective monitoring tools and preventive interventions, critical for achieving the ultimate goal of improving patient care and reducing healthcare burden. We have used a selective chemical labeling technique (5hmC-Seal) to profile genome-wide distributions of 5-hydroxymethylcytosines (5hmC), a gene activation mark, in patient-derived circulating cell-free DNA (cfDNA). Differentially modified 5hmC genes were identified across T2D patients with DN (n = 12), T2D patients with non-DN vascular complications (non-DN) (n = 29), and T2D patients with no complications (controls) (n = 14). Specifically, differential 5hmC markers between DN and controls revealed relevant pathways such as NOD-like receptor signaling pathway and tyrosine metabolism. A ten-gene panel was shown to provide differential 5hmC patterns between controls and DN, as well as between controls and non-DN patients using a machine learning approach. The 5hmC profiles in cfDNA reflected novel DN-associated epigenetic modifications relevant to the disease pathogenesis of DN. Importantly, these findings in cfDNA, a convenient liquid biopsy, have the potential to be exploited as a clinically useful tool for predicting DN in high risk T2D patients. Keywords: diabetes, nephropathy, epigenetics, 5-hydroxymethylcytosine, cfDNA
COVID 19 Pneumonia and a Rare Form of Fungal Peritonitis in a Patient Survivor on Peritoneal Dialysis

Abstract Peritoneal dialysis (PD) related peritonitis is usually caused by bacteria, but viruses and fungi could also affect the peritoneal membrane and cause cloudy effluent with negative bacterial cultures. We present a case of a PD patient who survived fungal peritonitis caused by Geotrichum klebahnii (March 2015) and COVID-19 pneumonia (April 2021) with peritonitis probably caused by the SARS-CoV-2 virus. The fungal peritonitis followed one episode of exit-site infection and two episodes of bacterial peritonitis treated with a wide-spectrum antibiotic. The patient’s PD catheter was removed immediately upon the diagnosis of fungal peritonitis, and an antifungal treatment was continued for 3 weeks after catheter removal. The new peritoneal catheter was reinserted 8 weeks after complete resolution of peritonitis, and the patient continued treatment with PD. The patient developed severe Covid-19 pneumonia with a sudden appearance of cloudy peritoneal effluent. There was no bacterial or fungal growth on the effluent culture. A PCR test for SARS-CoV-2 in peritoneal effluent was not performed. The peritoneal effluent became transparent with the resolution of the severe symptoms of Covid-19 pneumonia.
Circadian and diel regulation of photosynthesis in the bryophyte Marchantia polymorpha

Circadian rhythms are 24-hour biological cycles that align metabolism, physiology and development with daily environmental fluctuations. Photosynthetic processes are governed by the circadian clock in both flowering plants and some cyanobacteria, but it is unclear how extensively this is conserved throughout the green lineage. We investigated the contribution of circadian regulation to aspects of photosynthesis in Marchantia polymorpha, a liverwort that diverged from flowering plants early in the evolution of land plants. First, we identified in M. polymorpha the circadian regulation of photosynthetic biochemistry, measured using two approaches (delayed fluorescence, PAM fluorescence). Second, we identified that light-dark cycles synchronize the phase of 24 h cycles of photosynthesis in M. polymorpha, whereas the phases of different thalli desynchronize under free running conditions. This might also be due to masking of the underlying circadian rhythms of photosynthesis by light-dark cycles. Finally, we used a pharmacological approach to identify that chloroplast translation might be necessary for clock control of light harvesting in M. polymorpha. We infer that the circadian regulation of photosynthesis are well-conserved amongst terrestrial plants.
Perturbation of Alphavirus and Flavivirus Infectivity by Components of the Bacterial Cell Wall

During the past decades, the world has experienced a vast increase in epidemics of alphavirus and flavivirus infections. These viruses can cause severe diseases, such as hemorrhagic fever, encephalitis, and arthritis. ABSTRACT The impact of the host microbiota on arbovirus infections is currently not well understood. Arboviruses are viruses transmitted through the bites of infected arthropods, predominantly mosquitoes or ticks. The first site of arbovirus inoculation is the biting site in the host skin, which is colonized by a complex microbial community that could possibly influence arbovirus infection. We demonstrated that preincubation of arboviruses with certain components of the bacterial cell wall, including lipopolysaccharides (LPS) of some Gram-negative bacteria and lipoteichoic acids or peptidoglycan of certain Gram-positive bacteria, significantly reduced arbovirus infectivity in vitro. This inhibitory effect was observed for arboviruses of different virus families, including chikungunya virus of the Alphavirus genus and Zika virus of the Flavivirus genus, showing that this is a broad phenomenon. A modest inhibitory effect was observed following incubation with a panel of heat-inactivated bacteria, including bacteria residing on the skin. No viral inhibition was observed after preincubation of cells with LPS. Furthermore, a virucidal effect of LPS on viral particles was noticed by electron microscopy. Therefore, the main inhibitory mechanism seems to be due to a direct effect on the virus particles. Together, these results suggest that bacteria are able to decrease the infectivity of alphaviruses and flaviviruses. IMPORTANCE During the past decades, the world has experienced a vast increase in epidemics of alphavirus and flavivirus infections. These viruses can cause severe diseases, such as hemorrhagic fever, encephalitis, and arthritis. Several alpha- and flaviviruses, such as chikungunya virus, Zika virus, and dengue virus, are significant global health threats because of their high disease burden, their widespread (re-)emergence, and the lack of (good) anti-arboviral strategies. Despite the clear health burden, alphavirus and flavivirus infection and disease are not fully understood. A knowledge gap in the interplay between the host and the arbovirus is the potential interaction with host skin bacteria. Therefore, we studied the effect of (skin) bacteria and bacterial cell wall components on alphavirus and flavivirus infectivity in cell culture. Our results show that certain bacterial cell wall components markedly reduced viral infectivity by interacting directly with the virus particle.
Study of Calculating Methods for Earth Pressure Behind Abutment of Integral Abutment Bridge Under Action of Rising Temperatures

The calculating methods for earth pressure behind the abutment of integral abutment bridge under the action of rising temperatures are studied.The Broms method,m method and p—y curve method are respectively used to calculate the horizontal resistance coefficient of the filled earth behind the abutment back wall,the m method and p—y curve method are used to calculate the resistance coefficients of the earth around the abutment column and at the sides of the abutment and pier piles.The TDV software is used to simulate the restraint of the earth to the piles ends.Through the comparative analysis of the earth pressure calculated by the different calculation methods,it is known that when the earth pressure behind the abutment of the integral abutment bridge under the action of rising temperatures is calculated,it is not proper to use the m method to calculate the resistance coefficients of the abutment back wall and column earth while the resistance coefficient of the abutment back wall earth can be calculated by the Broms method.It is proper that the resistance coefficients of the earth of the abutment and pier piles should be considered by the p—y curve.
Responses to Song Playback Differ in Sleeping versus Anesthetized Songbirds

Abstract Vocal learning in songbirds is mediated by a highly localized system of interconnected forebrain regions, including recurrent loops that traverse the cortex, basal ganglia, and thalamus. This brain-behavior system provides a powerful model for elucidating mechanisms of vocal learning, with implications for learning speech in human infants, as well as for advancing our understanding of skill learning in general. A long history of experiments in this area has tested neural responses to playback of different song stimuli in anesthetized birds at different stages of vocal development. These studies have demonstrated selectivity for different song types that provide neural signatures of learning. In contrast to the ease of obtaining responses to song playback in anesthetized birds, song-evoked responses in awake birds are greatly reduced or absent, indicating that behavioral state is an important determinant of neural responsivity. Song-evoked responses can be elicited during sleep as well as anesthesia, and the selectivity of responses to song playback in adult birds is highly similar between anesthetized and sleeping states, encouraging the idea that anesthesia and sleep are similar. In contrast to that idea, we report evidence that cortical responses to song playback in juvenile zebra finches (Taeniopygia guttata) differ greatly between sleep and urethane anesthesia. This finding indicates that behavioral states differ in sleep versus anesthesia and raises questions about relationships between developmental changes in sleep activity, selectivity for different song types, and the neural substrate for vocal learning.
Application of Multimedia in Psychological Teaching Management

: For the current school education, the proportion of mental health education is increasing. With the promotion of education departments and schools, mental health education is getting more and more popular in schools. However, according to the current social survey, the strength and breadth of mental health education is not enough, and the actual teaching effect in the campus is not ideal. With the popularization and popularization of information and science and technology, multimedia is more and more widely used in various fields, especially in teaching, it can play a key role. It can intuitively present the voice of mental health education in front of students through video, pictures and audio broadcasting, so that students can be as personally as possible, and achieve very effective teaching effect in teaching management. This paper first introduces the main functions and connotations of multimedia teaching, and expounds the multi-faceted application of multimedia teaching; secondly, it expounds the significance of the application of multimedia teaching in psychological teaching management, and states what benefits it will bring to the application of multimedia in psychological teaching management. Finally, it puts forward many suggestions on the application of multimedia in Campus psychology teaching practice. The method of media situational teaching. Through a large number of investigations and interviews, the application of multimedia situational teaching in psychological teaching management proposed in this paper has good effect and practical feasibility, which proves that the application has good operability and student acceptance.
PERCEPTIVE PREDICTION OF MACHINE LEARNING OVER BIG DATA

: In the health care sector, large data collected every day and stored in databases to perform medical research and experiments. The challenge in health care sector is that for the same patient different doctors may diagnose in a different way. To overcome the challenges in healthcare sectors, automated classification tools are used to diagnose the disease accurately and efficiently and it may also reduce the time and burden on doctors. In this paper we analyze and compare the behaviour of five different classification algorithms namely Naive Bayes classifier, Decision tree algorithm, AdaBoost algorithm, K means clustering and Support Vector Machines on the given medical data sets. The datasets used for this work is an open source gathered from uci machine learning repository. The algorithms performance are measured based on eight criteria: Sensitivity, specificity, false positive rate, positive predictor value, negative predictor value, false discovery rate, accuracy and error rate.