The Effect of Various Concentrations of Adlay Seed Extract Polyelectrolyte Multilayers Film Coated on Titanium Surface to Hydrophilicity and Osteoblast Cell Viability and In Vitro Calcification

The titanium dental implants surface properties are highly related to osseointegration rate because they can interact with cells and other macromolecules. The polyelectrolyte multilayer (PEM) film coated on titanium surfaces is a method which could alter surface properties and change biological responses. The method to produce PEM film is to coat alternated layers between polyanion and polycation polymers combine with bioactive molecules as a final layer. This present study aims to fabricate PEM film by using Poly (diallyldimethylammonium chloride) or PDADMAC, poly (sodium 4-styrene sulfonate) or PSS, and adlay seed extract to assemble into PEM film of PDADMAC and adlay seed extracts in concentrations of 150, 300, 600, 1500, and 3000 μg/ ml; moreover, to study the surface characteristic in hydrophilic property as well as osteoblast cell adhesion, proliferation, and in vitro calcification. The hydrophilic property was evaluated by using water contact angle method, the adhesion and proliferation were observed by using MTT assay at 24, 48, and 72 hours of incubation periods, as well as, the in vitro calcification was analyzed with alizarin red staining at 14 and 21 days of incubation periods. The results showed that the titanium surfaces coated with PEM films of adlay seed extracts at concentration of 150, 300, 600, 1500 μg/ml had statistic significant higher surface hydrophilicity than uncoated titanium surface (p<0. 05) . The osteoblast cells could adhere and proliferate on all of specimen groups with time dependent manner. At 24 hours of incubation periods, there are cell numbers on PEM films of adlay seed extracts at concentrations of 600, 1500, and 3000 μg/ml had statistically lower than uncoated titanium surface (p<0. 05) . At 48 hours of incubation periods, PEM films of PDADMAC and adlay seed extracts at concentration of 150 μg/ml had statistic significant lower than uncoated titanium surface (p<0.05). There were no statistic significant differences of cell numbers on all specimen groups at 72 hours of incubation period (p>0. 05) . The in vitro calcification revealed the calcium depositions on every specimen groups, but there were no การประชุมวิชาการและน าเสนอผลงานวิจัยระดับชาติ ราชธานวีิชาการ คร้ังที่ 3 “นวัตกรรมที่พลิกโฉมสังคมโลก” ห น้ า | 1320 statistic significant differences of calcium deposition among all of specimen groups (p>0. 05) . In conclusions, the PEM films of adlay seed extract at concentrations of 150, 300, 600, and 1500 μg/ml could significantly increase the surface hydrophilicity. The osteoblast cell viability on every specimen groups can be observed at 24, 48, and 72 hours of incubation periods, but the significantly lower of cell numbers when compared to uncoated titanium could be observed on PEM films of adlay seed extracts at concentrations of 600, 1500, and 300 μg/ml at 24 hours of incubation periods, as well as PEM films of PDADMAC and adlay seed extract at concentration of 150 μg/ ml at 48 hours of incubation periods; in addition, the in vitro calcification at 14 and 21 days of incubation periods could observe the calcium depositions on every specimens, but there were no statistic significant differences among all specimen groups.