Miracle-dz/UniFAR-FTUs huggingface dataset:
“ DIRECT NOSE TO BRAIN DELIVERY OF NEUROTHERAPEUTICS-LOADED NANOEMULSION ”

The blood–brain barrier (BBB) plays a fundamental role in protecting the brain from toxic substances and therefore also controls and restricts the entry of therapeutic agents. The nasal administration of drugs using the nose-to-brain pathway allows direct drug targeting into the brain, avoiding the first-pass effect and bypassing the BBB. Through the nasal route, the drug can access the brain directly along the trigeminal and olfactory nerves, which are located in the upper part of the nasal cavity. Nanoemulsions are formulations belonging to the field of nanomedicine. They consist of emulsions (commonly oil in water) stabilized by one or more surfactants—and eventually co-surfactants— delivered in droplets of small dimensions (sizes of 100–300 nm or less) with a high surface area. A mucoadhesive polymer such as chitosan can be added to the formulation to impair rapid nasal clearance. Nanoemulsions represent promising formulations to deliver drugs directly into the brain through the intranasal route. Therefore, they can be used as a possible alternative to oral administration, avoiding problems such as low solubility in water, poor bioavailability, enzymatic degradation and slow onset of action. This review focuses the present situation in literature regarding the use of Nanoemulsions for nose-to-brain targeting, with particular attention to recent publications. Nasal Nanoemulsions appear to be effective, non-invasive and safe drug delivery systems to achieve brain targeting for the treatment of neurological diseases.
Differences in the Movement Skills and Physical Qualities of Elite Senior & Academy Rugby League Players Running Head: Movement Skills and Physical Qualities of Rugby League Players

The aim of the present study was to investigate (a) the differences in the movement skills and physical qualities between academy and senior rugby league players, and (b) the relationships between movement skills and physical qualities. Fiftyfive male rugby league players (Senior, n=18; Under 19 n=23; Under 16, n=14) undertook a physical testing battery including anthropometric (stature & body mass), strength (isometric mid-thigh pull; IMTP) and power (countermovement jump; CMJ) qualities, alongside the athletic ability assessment (AAA; comprised of overhead squat, double lunge, single-leg Romanian deadlift, press-up and pull-up exercises). Univariate analysis of variance demonstrated significant (p<0.001) differences in body mass, IMTP peak force, CMJ mean power, and AAA movement skills between groups. The greatest observed differences for total movement skills, peak force and mean power were identified between Under 16 and 19 academy age groups. Spearman’s rank correlation coefficients demonstrated a significant moderate (r=0.31) relationship between peak force and total movement skill. Furthermore, trivial (r=0.01) and small (r=0.13; r=0.22) relationships were observed between power qualities and total movement skill. These findings highlight that both movement skills and physical qualities differentiate between academy age groups, and provides comparative data for English senior and academy rugby league players.
Mechanism of Aluminum Inhibition of Net 45Ca2+ Uptake by Amaranthus Protoplasts' 1

Calcium ions serve as a second messenger in signal transduction and metabolic regulation. Effects of Al on calcium homeostasis remain to be elucidated. Short-term net "Ca2+ uptake by Amaranthus tricolor protoplasts was monitored from uptake media prepared to test the influence of pH, Al, and various inhibitors. Accumulation of "Ca2+ increased during the first 3 to 6 minutes and then leveled off or declined. Al and Ca2+ channel blockers (verapamil and bepridil) decreased net 4Ca2+ uptake. This decrease was more pronounced when Al and bepridil were both present in uptake media, but Al did not aggravate verapamil-induced reduction of net "Ca2+ uptake. Erythrosin B and calmidazolium each increased net 45Ca2+ uptake, probably by interfering with Ca2+ efflux. This effect was undetectable in the presence of Al. Mycophenolic acid decreased net "Ca2+ uptake; guanosine alleviated this effect. Al-induced reduction of net "Ca2+ uptake was not aggravated by mycophenolic acid. Net MCa2+ uptake was generally less at pH 4.5 than at 5.5 for all treatments. It is concluded that Al ions affect net "Ca2 uptake by binding to the verapamil-specific channel site that is different from the bepridil-specific one, as well as by interfering with the action of guanosine 5'-triphosphate-binding proteins.
Adapting typing for national laboratory-based surveillance of methicillin-resistant

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Adapting typing for national laboratory-based surveillance of methicillin-resistant A. Vainio, S. Koskela, A. Virolainen, J. Vuopio, S. Salmenlinna
Proximate compositional, texture profile and colour profile analysis of cottage cheeses prepared using milk clotting enzymes extracted from mustard and sunflower oilseed cakes

Through this study, the proximate composition, textural profile and colour profile parameters of cottage cheeses prepared using enzyme extracts from mustard and sunflower oilseed cakes (experimental cheese samples) were determined. Cottage cheese prepared using microbial rennet was taken as control. The results revealed that cottage cheeses developed using enzyme extracts were found highly comparable to the control sample when all the above mentioned parameters were studied. Thus, this study could conclude that these cheap plant sources of milk clotting enzymes (oilseed cakes) may prove a potential substitute of rennet in the dairy industry with probable applications. Further, intense evaluation of the quality of cheese curd produced by their action will shed more light into their commercial suitability
the evaluation of molecular typing methods for major food-borne microbiological hazards and their use for attribution modelling , outbreak investigation and scanning surveillance : Part 1 ( evaluation of methods and applications )

An evaluation of molecular typing methods that can be applied to the food-borne pathogens Salmonella, Campylobacter, Shiga toxin-producing Escherichia coli and Listeria monocytogenes is presented. This evaluation is divided in two parts. Firstly, commonly used molecular typing methods are assessed against a set of predefined criteria relating to discriminatory capacity, reproducibility, repeatability and current or potential suitability for international harmonisation. Secondly, the methods are evaluated for their appropriateness for use in different public health-related applications. These applications include outbreak detection and investigation, attribution modelling, the potential for early identification of food-borne strains with epidemic potential and the integration of the resulting data in risk assessment. The results of these evaluations provide updated insights into the use and potential for use of molecular characterisation methods, including whole genome sequencing technologies, in microbial food safety. Recommendations are also made in order to encourage a holistic and structured approach to the use of molecular characterisation methods for food-borne pathogens; in particular, on the importance of structured co-ordination at international level to help overcome current limitations in harmonisation of data analysis and interpretation. © European Food Safety Authority, 2013
CMAR_A_260129 9405..9415

Department of Emergency Surgery, Shaanxi Provincial People’s Hospital, Xi’an, Shaanxi Province, People’s Republic of China Background: The serious side effect of current conventional treatments for patients with metastatic colorectal cancer (CRC) highlights the requirement of an alternative treatment strategy. Natural compounds, such as curcumin, have been gained much attention due to its low toxicity and anti-tumor effect. Methods: qPCR and Western blot were used to measure the molecular changes induced by curcumin. Wound-healing assay and transwell assay were conducted to study the effect on cell migration and invasion. RT PCR array was performed to identify the miRNAs involved in curcumin-repressed EMT. Three algorithms and luciferase reporter assay were used to identify EPM5 as a target of miR-200c. The bioinformatical analysis of TCGA-COAD and other CRC cohorts were used to examine the association of EPM5 with EMT signatures and clinical relevance. The ectopic expression or siRNA-mediated knockdown of EPM5 was applied to study the role of EPM5 in CRC. Results: Treatment with curcumin changed the epithelial–mesenchymal transition (EMT)related gene expression, repressed cell migration and invasion in CRC cells. Its anti-tumor capability required the upregulation of miR-200c. EPM5 was a direct target of miR-200c and enriched in the consensus molecular subtype (CMS) 4 of CRC. Ectopic expression of EPM5 alone was sufficient to induce EMT in CRC. Downregulation of EPM5 was necessary for curcumin-repressed EMT, migration, and invasion. Higher expression of EPM5 was associated with the advanced TNM stages and poor survival in CRC. Conclusion: Our data provide the first evidence that the curcumin inhibits EMT in CRC by upregulation of miR-200c and downregulation of EPM5, and the use of curcumin might be able to prevent or delay CRC progression.
A Recommendation System in a Collaborative Digital Library Environment

We envisage a Digital Library not only as an information resource where users may submit queries to satisfy their information need, but also as a collaborative working and meeting space. We will present a personalized collaborative Digital Library environment, where users may organise the information space according to their own subjective view, may become aware of each other, exchange information and knowledge with each other, may build communities and may get recommendations. After formalising the main concepts of the personalized collaborative environment, major emphasis is given to the computational model for recommendation generation
GESTURING HELPS ENCODING VERBAL INFORMATION IN AMNESTIC MILD COGNITIVE IMPAIRMENT

Encoding in episodic memory is a step often impaired in patients with amnestic Mild Cognitive Impairment (aMCI). However, procedural memory processes are still relatively preserved. In line with previous research on the enactment effect, we investigated the potential benefit of encoding words combined with imitative gestures on episodic memory. Based on the Grober and Buschke’s free/cued recall procedure, we developed the Symbiosis test in which 13 patients with aMCI and 16 healthy elderly participants learned 32 words belonging to 16 different semantic categories either in a verbal encoding (A) or a bimodal (B; verbal and motor imitation) condition, using a blocked ABBA/BAAB procedure. Overall, memory retrieval was better in healthy participants than in patients with aMCI, and better for cued retrieval in the bimodal encoding (gesture cues) than the verbal encoding (category cues) condition, but there was no interaction effect between group and encoding conditions. These results show that performing concomitant gestures can enhance cued episodic memory retrieval in patients with aMCI as well as in healthy elderly controls. The Symbiosis test broadens the scope of the enactment effect, from action phrases to isolated words Author / title page
Cloud-based Infrastructure for Workﬂow and Service Engineering Using Semantic Web Technologies

—This paper presents the concept and implementation of a cloud-based infrastructure platform and tailored tools for graphical support for user-driven experiments. The focus lies on the creation of a platform that allows multiple users to integrate their expertise via Web service interfaces and combine them to compose workﬂows for their experiments in the engineering domain. The platform employs Semantic Web technologies, which increase interoperability of the services and assist during work-ﬂow design by suggesting compatible services. The implemented tools give users the possibility to utilize the platform using a standard Web browser, without any knowledge of service engineering and the underlying complex technologies. An exper-iment is described as a workﬂow and consists of orchestrated services from several software vendors that encapsulate speciﬁc tasks for improving product development. One advantage of this approach is the automatic execution of the services and data ﬂow among them. The cloud-based platform can also be combined with high-performance computing when services require complex calculations. The created platform offers optimal conditions to involve independent specialists and conduct short or long-running experiments, depending on the complexity of the task. This results in tremendous time savings and allows experts to carry out more experiments with products, which were omitted due to the complexity and the limited computing power, until now. The possibility to conduct these experiments improves the productive know-how of the companies and enhances the products they are selling.
Association between Auditory Memory and Performance on Dichotic Digit Test

Background: Present study was designed to investigate the effect of auditory memory on the performance of SDT and DDT for Marathi speaking typically developing children and children at risk for CAPD in the age range of 6 to 8 years. Method: A total 60 typically developing children and nine children with risk for central auditory processing disorder were compared on performance of Single digit dichotic test (SDT) and Double Dichotic Digit Test (DDT) in Marathi. Single correct scores for right ear (RS); left ear (LS); double correct scores (DS); and ear difference (ED) were calculated separately. Results: All participants showed better performance on SDT than those of DDT, for all the parameters. Children at risk for CAPD performed significantly poor on single correct score of right ear and ear difference of DDT. Result of Spearman’s correlation showed significant moderate correlation between memory span and single correct score of left ear, double correct score of DDT among typically developing children. Performance on single correct score of left ear and double correct score of the DDT of participants of children with memory span four or more than four was significantly better than those of children with memory span less than four. Conclusion: Present study suggests that for children in the age range of 6 to 8 years DDT is better tool than SDT in detection binaural integration deficits. Evidences obtained from this study support the notion that auditory memory affects the performance of the dichotic listening skills.
Simulation Analysis and Electromagnetic Dosimetry of Patch Antenna on Hugo Voxel Model

The off-body, on-body simulation analysis and electromagnetic dosimetry of a simple structure patch antenna operating in 2.45 GHz ISM band is presented. The antenna design is based on conventional structure with flexible substrate features and copper as the conductive plane. The simulation analysis was performed in CST Microwave Studio. Anatomic simulation using HUGO voxel model was used for on-body simulation and electromagnetic dosimetry. For off-body analysis, the antenna resonates at 2.45 GHz with S11 parameter of -32.56 dB and radiates unidirectionally with efficiency of 91.96 %. It was found that the presence of human lossy tissues and organs caused frequency detuning of 50.4-51.5 MHz and radiation efficiency degradation of 7.93 % to 13.78 %. The electromagnetic dosimetry on specific absorption rate (SAR) exposure of the antenna when it is mounted over averaged 10 g of tissues for chest, back, abdomen, arm and thigh was well below the IEEE Std. C95.3 limit. The maximum and minimum SAR was found when the antenna was placed on the back (0.332 W/kg) and arm (0.121 W/kg) respectively on the human body. Varying the distance from 0-20 mm of the antenna from the human body reduces the SAR exposure to the human body.
A protocol for systematic review and meta-analysis

Background: Neonates with moderate to severe encephalopathy benefit significantly from therapeutic hypothermia, with reduced risk of death or disability. However, the need for therapeutic hypothermia for mild neonatal encephalopathy (NE) remains unclear. Therefore, we conducted a protocol for systematic review and meta-analysis to provide evidence supporting therapeutic hypothermia for term or near term neonates with mild NE, including findings of recent long-term outcome studies, as well as novel adjunctive therapies to augment neurodevelopmental outcomes for neonates with NE who receive therapeutic hypothermia. Methods: Two independent researchers performed a systematic literature search in different electronic databases including PubMed, the Cochrane Center Controlled Trials Register, EMBASE, Medline, Ovid, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database, andWanfang Database without any restrictions of languages and date. Two reviewers will screen the records and include quality studies according to inclusion criteria independently. Two reviewers will assess the risk of bias of the included studies by the “Risk of Bias Assessment Tool” of the Cochrane Handbook for randomized controlled trials. Statistical analysis will be performed with Review Manager software 5.3. Results: A synthesis of current evidence of therapeutic hypothermia for treating mild NE will be provided in this protocol. Conclusion: The results of this study will provide a theoretical basis for the clinical use of therapeutic hypothermia in mild NE. Abbreviations: NE = neonatal encephalopathy, RCT = randomized controlled trial.
F-florbetapir positron emission tomography of late mild cognitive impairment

Background: Semiquantitative methods such as the standardized uptake value ratio (SUVR) require normalization of the radiotracer activity to a reference tissue to monitor changes in the accumulation of amyloid-β (Aβ) plaques measured with positron emission tomography (PET). The objective of this study was to evaluate the effect of reference tissue normalization in a test–retest F-florbetapir SUVR study using cerebellar gray matter, white matter (two different segmentation masks), brainstem, and corpus callosum as reference regions. Methods: We calculated the correlation between F-florbetapir PET and concurrent cerebrospinal fluid (CSF) Aβ1–42 levels in a late mild cognitive impairment cohort with longitudinal PET and CSF data over the course of 2 years. In addition to conventional SUVR analysis using mean and median values of normalized brain radiotracer activity, we investigated a new image analysis technique—the weighted two-point correlation function (wS2)—to capture potentially more subtle changes in Aβ-PET data. Results: Compared with the SUVRs normalized to cerebellar gray matter, all cerebral-to-white matter normalization schemes resulted in a higher inverse correlation between PET and CSF Aβ1–42, while the brainstem normalization gave the best results (high and most stable correlation). Compared with the SUVR mean and median values, the wS2 values were associated with the lowest coefficient of variation and highest inverse correlation to CSF Aβ1–42 levels across all time points and reference regions, including the cerebellar gray matter. Conclusions: The selection of reference tissue for normalization and the choice of image analysis method can affect changes in cortical F-florbetapir uptake in longitudinal studies.
Avant-garde model of motor abilities

It was defined and introduced a new model of motor functions determined with continual interaction of entity and its environment. All events are dynamical with changes into three possible ways: a) strengthening of importance of acute events and reactions (On-line acting) with possible entity destruction, b) increase of importance of cooperation with other entities from environment (Common acting) with sharing information and resources, c) consolidation of internal permanent accumulation of entity (Batch acting) with strengthening of individual abilities and opening new spaces and development categories.
Draft Genome Sequences of Stenotrophomonas maltophilia Strains Sm32COP, Sm41DVV, Sm46PAILV, SmF3, SmF22, SmSOFb1, and SmCVFa1, Isolated from Different Manures in France: TABLE 1

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Draft Genome Sequences of Stenotrophomonas maltophilia Strains Sm32COP, Sm41DVV, Sm46PAILV, SmF3, SmF22, SmSOFb1, and SmCVFa1, Isolated from Different Manures in France: TABLE 1 Josselin Bodilis, Benjamin Youenou, Jerome Briolay, Elisabeth Brothier, Sabine Favre-Bonté, Sylvie Nazaret
An Investigation of Pulsatile Blood Flow in An Angulated Neck of Abdominal Aortic Aneurysm Using Computational Fluid Dynamics

Article history: Received 1 February 2019 Received in revised form 18 April 2019 Accepted 2 May 2019 Available online 17 May 2019 Hemodynamics in highly proximal neck of abdominal aortic aneurysm (AAA) was investigated in an idealized case by using computational fluid dynamics (CFD) technique. The steady and pulsatile flow simulations were carried out and compared. The three-dimensional model was constructed using CAD software and boundary conditions were adopted from the literature based on experimental studies. Grid independency study was performed to test the computational mesh used. Blood was considered as incompressible and Newtonian fluid in steady and transient regimes with no-slip and rigid aortic wall. Finite volume method based on ANSYS Fluent was used to solve the CFD governing equations. The results acquired from CFD post-processing for velocity distributions and flow rate during steady and transient (t = 0.25 s and t = 0.55 s) flow states. Furthermore, velocity distributions showed significant differences between the two cases (steady and transient) in the idealized angulated neck AAA model. During the steady state, the flow was concentrated in the middle of aorta while in transient (a systolic phase) the flow was more pronounced and distributed near the wall and tortuous areas more than in a diastolic phase. Flow rate at iliac artery outlets showed different values in both states and slight variance in the right and left iliac arteries. The steady and pulsatile flow comparison using CFD were crucial to understand and investigate the hemodynamic in angulated neck of abdominal aortic aneurysm model.