Miracle-dz/UniFAR-FTUs huggingface dataset:
Fission yeast Ish1 and Les1 interact with each other in the lumen of the nuclear envelope

The nuclear envelope (NE) provides a permeable barrier that separates the eukaryotic genome from the cytoplasm. NE is a double membrane composed of inner and outer nuclear membranes. Ish1 is a stress‐responsive NE protein in the fission yeast, Schizosaccharomyces pombe. Les1 is another NE protein that shares several similar domains with Ish1, but the relationship between them remains unknown. In this study, using fluorescence and electron microscopy, we found that most regions of these proteins were localized within the NE lumen. We also found that Ish1 interacted with Les1 via its C‐terminal region in the NE lumen and that the NE localization of Ish1 depended on the C‐terminal region of Les1. Ish1 and Les1 were co‐localized at the NE in interphase cells, but when the nucleus divided at the end of mitosis (closed mitosis), they showed distinguishable localization at the midzone membrane domain. These results suggest the regulated interaction between Ish1 and Les1 in the NE lumen, although this interaction does not appear to be essential for cell survival.
The Use of IV Vasoactive Intestinal Peptide (Aviptadil) in Patients With Critical COVID-19 Respiratory Failure: Results of a 60-Day Randomized Controlled Trial*

OBJECTIVES: Respiratory failure is a lethal complication of COVID-19 that has remained resistant to drug therapy. Vasoactive intestinal peptide (VIP) is shown in nonclinical studies to upregulate surfactant production, inhibit cytokine synthesis, prevent cytopathy, and block replication of the severe acute respiratory syndrome coronavirus 2 virus in pulmonary cells. The study aims to determine whether Aviptadil (synthetic VIP) can improve survival and recovery in patients with COVID-19 respiratory failure compared with placebo and demonstrate biological effects in such patients. DESIGN: A multicenter, placebo-controlled trial. SETTING: Ten U.S. hospitals: six tertiary-care hospitals and four community hospitals. PATIENTS: A total of 196 patients with COVID-19 respiratory failure. INTERVENTIONS: Participants were randomized 2:1 to receive 3 days of IV Aviptadil or placebo. MEASUREMENTS AND MAIN RESULTS: The primary end point (alive and free from respiratory failure at day 60) did not reach statistical significance (odds ratio [OR], 1.6; 95% CI, 0.86–3.11) for patients treated with Aviptadil when controlling for baseline ventilation status as prespecified in the protocol. There was, however, a statistically significant two-fold odds of improved survival (OR, 2.0; 95% CI, 1.1–3.9) at 60 days (p = 0.035). There was significant improvement in respiratory distress ratio and reduced interleukin 6 cytokine release (p = 0.02) by day 3. Subgroup analysis identified a statistically significant likelihood of achieving primary end point among those treated with high-flow nasal oxygen at baseline (p = 0.039). Subjects on mechanical ventilation also experienced a 10-fold increased odds of survival with drug versus placebo (p = 0.031). CONCLUSIONS: The primary end point did not reach statistical significance, indicating that there was no difference between Aviptadil versus placebo. However, Aviptadil improves the likelihood of survival from respiratory failure at day 60 in critical COVID-19 across all sites of care. Given the absence of drug-related serious adverse events and acceptable safety profile, we believe the benefit versus risk for the use of Aviptadil is favorable for patient treatment.
Redesigning of 3-Dimensional Vascular-Muscle Structure Using ADSCs/HUVECs Co-Culture and VEGF on Engineered Skeletal Muscle ECM

Objective The main objective of this study is to determine the myogenic effects of skeletal muscle extracellular matrix, vascular endothelial growth factor and human umbilical vein endothelial cells on adipose-derived stem cells to achieve a 3-dimensional engineered vascular-muscle structure. Materials and Methods The present experimental research was designed based on two main groups, i.e. monoculture of adipose tissue-derived stem cells (ADSCs) and co-culture of ADSCs and human umbilical vein endothelial cells (HUVECs) in a ratio of 1:1. Skeletal muscle tissue was isolated, decellularized and its surface was electrospun using polycaprolactone/gelatin parallel nanofibers and then matrix topography was evaluated through H&E, trichrome staining and SEM. The expression of MyHC2 gene and tropomyosin protein were examined through real-time reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence, respectively. Finally, the morphology of mesenchymal and endothelial cells and their relationship with each other and with the engineered scaffold were examined by scanning electron microscopy (SEM). Results According to H&E and Masson’s Trichrome staining, muscle tissue was completely decellularized. SEM showed parallel Polycaprolactone (PCL)/gelatin nanofibers with an average diameter of about 300 nm. The immunofluorescence proved that tropomyosin was positive in the ADSCs monoculture and the ADSCs/HUVECs co- culture in horse serum (HS) and HS/VEGF groups. There was a significant difference in the expression of the MyHC2 gene between the ADSCs and ADSCs/HUVECs culture groups (P<0.05) and between the 2D and 3D models in HS/ VEGF differentiation groups (P<001). Moreover, a significant increase existed between the HS/VEGF group and other groups in terms of endothelial cells growth and proliferation as well as their relationship with differentiated myoblasts (P<0.05). Conclusion Co-culture of ADSCs/HUVECs on the engineered cell-free muscle scaffold and the dual effects of VEGF can lead to formation of a favorable engineered vascular-muscular tissue. These engineered structures can be used as an acceptable tool for tissue implantation in muscle injuries and regeneration, especially in challenging injuries such as volumetric muscle loss, which also require vascular repair.
Automated Construction of Lexicons to Improve Depression Screening With Text Messages

Given that depression is one of the most prevalent mental illnesses, developing effective and unobtrusive diagnosis tools is of great importance. Recent work that screens for depression with text messages leverage models relying on lexical category features. Given the colloquial nature of text messages, the performance of these models may be limited by formal lexicons. We thus propose a strategy to automatically construct alternative lexicons that contain more relevant and colloquial terms. Specifically, we generate 36 lexicons from fiction, forum, and news corpuses. These lexicons are then used to extract lexical category features from the text messages. We utilize machine learning models to compare the depression screening capabilities of these lexical category features. Out of our 36 constructed lexicons, 14 achieved statistically significantly higher average F1 scores over the pre-existing formal lexicon and basic bag-of-words approach. In comparison to the pre-existing lexicon, our best performing lexicon increased the average F1 scores by 10%. We thus confirm our hypothesis that less formal lexicons can improve the performance of classification models that screen for depression with text messages. By providing our automatically constructed lexicons, we aid future machine learning research that leverages less formal text.
Recognize the Image of the Inscription at the Bottom of Purple Clay Teapot Using Improved Sift Algorithm

We use an improvement to the conventional sift algorithm to accurately recognize the image of the inscription at the bottom of purple clay teapot. We show that the improvement causes the false match rate of the image to be greatly reduced, and that the improvement meets the requirement of high-precision project such as purple clay teapot. We propose a method of adaptively selecting stable points. The feature points extracted in this way have smaller changes and are more stable with scale changes, which is more conducive to image matching. We extensively test our algorithm using a dataset of one hundred sets of images.
Ultrasound and surface electromyography analyses reveal an intensity dependent active stretch-shortening cycle of the vastus lateralis muscle during ergometer rowing

ABSTRACT A rowing cycle is characterised by a stretch-shortening cycle (SSC) at the quadriceps femoris muscle-tendon unit (MTU) level. However, due to the associated decoupling between MTU and muscle fascicle length changes, it remains unclear whether a rowing cycle causes active stretch at the muscle level. Fifteen young, sub-elite, male rowers (19.5 ± 1.6 yr; 1.94 ± 0.06 m; 91.9 ± 5.4 kg; rowing experience: 7.5 ± 2.8 yr) performed randomised 60-s rowing intervals using a traditional style at a low (LiR) and high intensity (HiR) and a micro-pause style at a low intensity (MpR). Muscle activity, knee joint angles, and muscle fascicle length changes from the left-sided vastus lateralis (VL) muscle were quantified using surface electromyography, inertial measurement units, and B-mode ultrasound imaging, respectively. All rowing conditions showed active fascicle stretch during late knee flexion (p≤0.001, standardised mean difference (SMD) ≥0.72) and subsequent active fascicle shortening throughout knee extension. Active fascicle stretch duration, amplitude and velocity (rANOVA: p≤0.001, ηp 2 = 0.49) were not significantly different (p≥0.174; SMD≤0.26) between LiR and MpR, but were significantly increased during HiR (p≤0.001; SMD≥0.70). The percentage of rowing cycles that involved active fascicle stretch (rANOVA: p≤0.001, ηp 2 = 0.95; post-hoc: p≤0.001, SMD≥0.87) was also significantly higher for HiR (98.3 ±12.9%) compared with both LiR (65.0 ± 48.1%) and MpR (68.3 ± 46.9%). In conclusion, rowing involves SSC at the VL muscle fascicle level, but the amount of active stretch differs between rowing intensities, with the longest, largest, and fastest active stretch occurring during HiR. SSC-based mechanisms may therefore contribute more to rowing performance during HiR than LiR or MpR. Highlights Surface electromyography and ultrasound imaging revealed stretch-shortening cycles (SSCs) of the vastus lateralis muscle fascicles during rowing Increased active fascicle stretch duration, amplitude and velocity from low- to high-intensity rowing indicate that SSC-based mechanisms likely contribute more to performance during high-intensity rowing The SSC within the vastus lateralis muscle was independent of the rowing style at the same low rowing intensity
A Telecare System that Estimates Quality of Life through Communication

Quality of life (QoL) is an effective index of well-being, including physical health as well as mental state of individuals. In this paper, audio and video data were collected from healthy participants during a natural language conversation with a conversational agent. This yielded ten hours of audio and video data. A system that uses a deep learning architecture was constructed to estimate the user’s QoL. This system was built using a 3D convolutional neural network and an SVM for multimodal data. In order to evaluate the accuracy of the estimation system, two experiments were conducted. In the first experiment, the QoL question-answer estimation experiment, the accuracy of “Physical functioning”, “Role physical”, “Body pain”, “General health”, “Vitality”, “ Social functioning”, “Role emotional”, and “Mental Health” scale was 84.0%, 68.0%, 67.8%, 62.3%, 51.3%, 68.0%, 54.8% and 47.0% in order. The total average of the correct answer rate was 65%. In the second experiment, the QoL score-regression experiment, the distribution of the difference between the actual score and the estimated results, known as error, was investigated. These results imply that the features necessary for QoL estimation can be extracted from audio and video data except for the “Mental Health” domain. These results also show the usefulness of the estimation system using multimodal data. Finally, suggestions and discussions regarding the plausible behavior of the estimation results were made from the viewpoint of human agent interaction in the field of elderly welfare. From the results, we concluded that it is possible to extract high dimensional information regarding the QoL of a human including their satisfaction level towards daily life and social activities from multimodal data.
Social determinants of health associated with psychological distress stratified by lifetime traumatic brain injury status and sex: Cross-sectional evidence from a population sample of adults in Ontario, Canada

This study identified the social determinants of health (SDoH) associated with psychological distress in adults with and without a self-reported history of traumatic brain injury (TBI), stratified by sex. Data from the 2014–2017 cycles of the Centre for Addiction and Mental Health Monitor Survey, a representative survey of adults ≥18 years in Ontario, Canada, were analyzed (N = 7,214). The six-item version of the Kessler Psychological Distress Scale was used to determine moderate to severe psychological distress. Self-reported lifetime TBI was defined as a head injury resulting in a loss of consciousness for ≥5 minutes or at least one-night stay in the hospital (16.4%). Among individuals reporting a history of TBI, 30.2% of males and 40.1% of females reported psychological distress (p = 0.0109). Among individuals who did not report a history of TBI, 17.9% of males and 23.5% of females reported psychological distress (p<0.0001). Multivariable logistic regression analyses showed that the SDoH significantly associated with elevated psychological distress were similar between individuals with and without a history of TBI. This included unemployment, student, or ‘other’ employment status among both males and females; income below the provincial median and age 65+ among males; and rural residence among females. This study highlighted opportunities for targeted population-level interventions, namely accessible and affordable mental health supports for individuals with lower income. Notably, this study presented evidence suggesting adaptations to existing services to accommodate challenges associated with TBI should be explored, given the finite and competing demands for mental health care and resources.
The relationship between age at first drink and later risk behaviours during a period of youth drinking decline

Abstract Aims Alcohol consumption among teenagers in many high‐income countries has steadily declined since the early 2000s. There has also been a steady increase in the average age at first drink, a reliable marker of later alcohol problems. This study measured whether young people who initiated drinking early were at increased risk of alcohol problems in young adulthood in recent cohorts where early initiation was comparatively rare. Design Analysis of six waves of a repeated cross‐sectional household survey (2001–16). Setting Australia. Participants A total of 9576 young adults (aged 20–25 years) who had initiated drinking before the age of 20 years. Measurements Respondents were classified into three groups based on their self‐reported age at first drink (< 16, 16–17, 18–19 years). Outcome variables were self‐reported experiences of memory loss while drinking, risky and delinquent behaviour while drinking and monthly or more frequent drinking occasions of 11 or more 10‐g standard drinks. Findings Later initiators reported lower levels of all outcomes [e.g. odds ratios (ORs) for memory loss were 0.56, 95% confidence interval (CI) = 0.50, 0.63] for those who first drank at 16 or 17 years compared with those who first drank at age 15 or younger). Significant interactions between age at first drink and survey year showed that early initiation was more strongly associated with harms (e.g. for memory loss, OR = 0.97, 95% CI = 0.94, 0.99 for 18–19 versus 15 or younger) in young adulthood for recent cohorts where early drinking was less common. Conclusions The decline in youth drinking may have contributed to a concentration of risk of alcohol problems among those young people who consume alcohol in early adolescence. Early initiation of drinking may be an increasingly important marker of broader risk taking as alcohol becomes less normative for teenagers.
Quantum Dynamics for Ion Channel Transport, Poisson-Schrödinger Modell

Abstract. This paper deals with the mathematical model of the ion permeation in potassium channels of biomembrane. Based on the Hamiltons, variational principle was led out to the set of compiling equations describing quantum dynamics of the potassium ion transport; Poisson-Schrodinger equation for electric potential φ(r, t), and Schrodinger equation for wave function ψ(r, t). Received the set of equations was formulated in the form of two variational identities. A numerical algorithm of the solution, was proposed, based on the meshles Galerkin approximation.
Long-Term Trends in Secondhand Smoke Exposure in High-Rise Housing Serving Low-Income Residents in New York City: Three-Year Evaluation of a Federal Smoking Ban in Public Housing, 2018–2021

Abstract Introduction In July 2018, the U.S. Department of Housing and Urban Development passed a rule requiring public housing authorities to implement smoke-free housing (SFH) policies. We measured secondhand smoke (SHS) exposure immediately before, and repeatedly up to 36 months post-SFH policy implementation in a purposeful sample of 21 New York City (NYC) high-rise buildings (>15 floors): 10 NYC Housing Authority (NYCHA) buildings subject to the policy and 11 privately managed buildings in which most residents received housing vouchers (herein “Section 8”). Aims and Methods We invited participants from nonsmoking households (NYCHA n = 157, Section-8 n = 118) to enroll in a longitudinal air monitoring study, measuring (1) nicotine concentration with passive, bisulfate-coated filters, and (2) particulate matter (PM2.5) with low-cost particle sensors. We also measured nicotine concentrations and counted cigarette butts in common areas (n = 91 stairwells and hallways). We repeated air monitoring sessions in households and common areas every 6 months, totaling six post-policy sessions. Results After 3 years, we observed larger declines in nicotine concentration in NYCHA hallways than in Section-8, [difference-in-difference (DID) = −1.92 µg/m3 (95% CI –2.98, −0.87), p = . 001]. In stairwells, nicotine concentration declines were larger in NYCHA buildings, but the differences were not statistically significant [DID= −1.10 µg/m3 (95% CI −2.40, 0.18), p = . 089]. In households, there was no differential change in nicotine concentration (p = .093) or in PM2.5 levels (p = .385). Conclusions Nicotine concentration reductions in NYCHA common areas over 3 years may be attributable to the SFH policy, reflecting its gradual implementation over this time. Implications Continued air monitoring over multiple years has demonstrated that SHS exposure may be declining more rapidly in NYCHA common areas as a result of SFH policy adherence. This may have positive implications for improved health outcomes among those living in public housing, but additional tracking of air quality and studies of health outcomes are needed. Ongoing efforts by NYCHA to integrate the SFH policy into wider healthier-homes initiatives may increase policy compliance.
Research on the Control of Cascade Multilevel Hybrid Energy Storage System Based on Voltage Source/Current Source

In the cascade hybrid energy storage system, if energy storage battery fails, an asymmetric converter based on batteries and capacitors can be formed by disconnecting the contact switch of the fault battery. A novel control method based on voltage source/current source is proposed for this asymmetric system. The battery chains can be equivalent to voltage source, and the capacitor chains can be equivalent to current source. To solve the problem of imbalance between the battery units and capacitor units, a balancing control method is applied through adding an active power voltage vector based on the hierarchical control architecture. Steady state operation area and operation region of the proposed control method are also analyzed. The correctness and validity of the method are verified by simulation.
Comparative Analysis of Clinical and Genomic Characteristics of Hypervirulent Klebsiella pneumoniae from Hospital and Community Settings: Experience from a Tertiary Healthcare Center in India

To date, studies comparing the genomic characteristics of hospital- and community-acquired hvKp were very few in India. In this study, we analyzed the clinical and genomic characteristics of hvKp isolates from hospital and community settings. ABSTRACT Hypervirulent Klebsiella pneumoniae (hvKp) is a hypermucoviscous phenotype of classical Klebsiella pneumoniae (cKp) that causes serious infections in the community. The recent emergence of multidrug-resistant hvKp isolates (producing extended-spectrum beta-lactamases and carbapenemases) along with other virulence factors in health care settings has become a clinical crisis. Here, we aimed to compare the distribution of virulence determinants and antimicrobial resistance (AMR) genes in relation to various sequence types (STs) among the clinical hvKp isolates from both settings, to reinforce our understanding of their epidemiology and pathogenic potential. A total of 120 K. pneumoniae isolates confirmed by matrix-assisted laser desorption ionization–time of flight mass spectrometry were selected. hvKp was phenotypically identified by string test and genotypically confirmed by the presence of the iucA gene using PCR. Molecular characterization of hvKp isolates was done by whole-genome sequencing (WGS). Of the K. pneumoniae isolates, 11.6% (14/120) isolates were confirmed as hvKp by PCR (9.1% [11/120] string positive and 3.3% [4/120] positive by both methods); these were predominantly isolated from bloodstream infection (50%, 7/14), urinary tract infection (29%, 4/14), and respiratory tract infection (21%, 3/14). For all 14 hvKp infections, for 14.2% the source was in the community and for 85.7% the source was a health care setting. Two virulent plasmids were identified by WGS among the hvKp isolates from both settings. K64 was found to be the commonest capsular serotype (28.5%, 4/14), and ST2096 was the most common ST (28.5%, 4/14) by WGS. Two new STs were revealed: ST231 (reported to cause outbreaks) and ST43. The genome of one isolate was determined to be carrying AMR genes (blaCTX-M-15, blaNDM-1, blaNDM-5, blaOXA-181, blaOXA-232, etc.) in addition to virulence genes, highlighting the clonal spread of hvKp in both community and health care settings. IMPORTANCE To date, studies comparing the genomic characteristics of hospital- and community-acquired hvKp were very few in India. In this study, we analyzed the clinical and genomic characteristics of hvKp isolates from hospital and community settings. ST2096 was found as the most common ST along with novel STs ST231 and ST43. Our study also revealed the genome is simultaneously carrying AMR as well as virulence genes in isolates from both settings, highlighting the emergence of MDR hvKp STs integrated with virulence genes in both community and health care settings. Thus, hvKp may present a serious global threat, and essential steps are needed to prevent its further dissemination.