Miracle-dz/UniFAR-FTUs huggingface dataset:
A prospective population-based study of health complaints in adolescence and use of social welfare benefits in young adulthood

Background: There is a lack of knowledge on how health problems in adolescence are connected to work marginalization in adulthood. The aim of this study was to study work marginalization in young adulthood, measured by use of long-term social welfare benefits, and its associations with self-reported health complaints, total symptom burden and self-rated general health at ages 15–16. Methods: We linked data from a youth health survey conducted during 1999–2004 to data from Norwegian registries that followed each participant through February 2010. Cox regression analysis was used to compute hazard ratios (HRs) for the use of long-term social welfare benefits in young adulthood, based on health measures in 15–16 year-olds. Results: During the follow-up, 17% of the study population received some sort of long-term social welfare benefit. In the baseline survey, 95% of the adolescents reported one or more health complaints. The mean number of health complaints was 4.8. Girls reported a significantly higher mean number of complaints (5.7) than did boys (3.8) (p < 0.001). Several individual health complaints were associated with an increased use of long-term social welfare benefits. We found an increasing relative hazard of social welfare usage, depending on the number of complaints reported at baseline. Ill self-perceived general health was found to be strongly associated with the use of benefits during the follow-up: In girls, we found HRs of 1.41 (CI 1.21–1.65), 2.76 (2.29–3.31) and 2.77 (1.51–5.07) for those with good, not so good and bad health, respectively; compared to very good health. The corresponding numbers in boys were 1.41 (1.25–1.59), 1.93 (1.60–2.32) and 1.31 (0.72–2.38), respectively. Conclusions: Several health measures in adolescents were found to be associated with future work marginalization in young adulthood. The associations remained significant, even after correcting for such strong predictors as the parents’ education and family economy. Total symptom burden and self-perceived general health can add additional knowledge onto how adolescent health is related to work marginalization, in a longitudinal perspective.
/spl alpha/-robust H/sub /spl infin// state feedback control for a class of linear parameter-varying systems

In this paper, the design problem of /spl alpha/-robust H/sub /spl infin// state feedback controller for a class of linear parameter-varying (LPV) systems with time-delay is addressed. A sufficient condition for the existence of /spl alpha/-robust H/sub /spl infin//m state feedback controller is derived in terms of a new method and the corresponding design method of the controller is given. Furthermore, illustrative example is given to demonstrate the superiority of the presented method.
MgtE Homolog FicI Acts as a Secondary Ferrous Iron Importer in Shewanella oneidensis Strain MR-1

ABSTRACT The transport of metals into and out of cells is necessary for the maintenance of appropriate intracellular concentrations. Metals are needed for incorporation into metalloproteins but become toxic at higher concentrations. Many metal transport proteins have been discovered in bacteria, including the Mg2+ transporter E (MgtE) family of passive Mg2+/Co2+ cation-selective channels. Low sequence identity exists between members of the MgtE family, indicating that substrate specificity may differ among MgtE transporters. Under anoxic conditions, dissimilatory metal-reducing bacteria, such as Shewanella and Geobacter species, are exposed to high levels of soluble metals, including Fe2+ and Mn2+. Here we characterize SO_3966, which encodes an MgtE homolog in Shewanella oneidensis that we name FicI (ferrous iron and cobalt importer) based on its role in maintaining metal homeostasis. A SO_3966 deletion mutant exhibits enhanced growth over that of the wild type under conditions with high Fe2+ or Co2+ concentrations but exhibits wild-type Mg2+ transport and retention phenotypes. Conversely, deletion of feoB, which encodes an energy-dependent Fe2+ importer, causes a growth defect under conditions of low Fe2+ concentrations but not high Fe2+ concentrations. We propose that FicI represents a secondary, less energy-dependent mechanism for iron uptake by S. oneidensis under high Fe2+ concentrations. IMPORTANCE Shewanella oneidensis MR-1 is a target of microbial engineering for potential uses in biotechnology and the bioremediation of heavy-metal-contaminated environments. A full understanding of the ways in which S. oneidensis interacts with metals, including the means by which it transports metal ions, is important for optimal genetic engineering of this and other organisms for biotechnology purposes such as biosorption. The MgtE family of metal importers has been described previously as Mg2+ and Co2+ transporters. This work broadens that designation with the discovery of an MgtE homolog in S. oneidensis that imports Fe2+ but not Mg2+. The research presented here also expands our knowledge of the means by which microorganisms have adapted to take up essential nutrients such as iron under various conditions.
Self-reported psychological demands, skill discretion and decision authority at work: A twin study

Objectives: To examine the contribution of genetic factors to self-reported psychological demands (PD), skill discretion (SD) and decision authority (DA) and the possible importance of such influence on the association between these work variables and depressive symptoms. Methods: 11,543 participants aged 27–54 in the Swedish Twin Registry participated in a web survey. First of all, in multiple regressions, phenotypic associations between each one of the three work environment variables and depressive symptoms were analysed. Secondly, by means of classical twin analysis, the genetic contribution to PD, SD and DA was assessed. After this, cross-twin cross-trait correlations were computed between PD, SD and DA, on the one hand, and depressive symptom score, on the other hand. Results: The genetic contribution to self-reported PD, DS and DA ranged from 18% for decision authority to 30% for skill discretion. Cross-twin cross-trait correlations were very weak (r values < .1) and non-significant for dizygotic twins, and we lacked power to analyse the genetic architecture of the phenotypic associations using bivariate twin modelling. However, substantial genetic contribution to these associations seems unlikely. Conclusions: Genetic contributions to the self-reported work environment scores were 18–30%.
A prática de enfermagem baseada em evidências: conceitos e informações disponíveis online

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Construction and Optimization of a Heterologous Pathway for Protocatechuate Catabolism in Escherichia coli Enables Bioconversion of Model Aromatic Compounds

ABSTRACT The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to catabolize lignin-derived aromatic compounds and to generate valuable products. While Escherichia coli has been engineered to produce a variety of fuels and chemicals, it is incapable of catabolizing most aromatic compounds. Therefore, we engineered E. coli to catabolize protocatechuate, a common intermediate in lignin degradation, as the sole source of carbon and energy via heterologous expression of a nine-gene pathway from Pseudomonas putida KT2440. We next used experimental evolution to select for mutations that increased growth with protocatechuate more than 2-fold. Increasing the strength of a single ribosome binding site in the heterologous pathway was sufficient to recapitulate the increased growth. After optimization of the core pathway, we extended the pathway to enable catabolism of a second model compound, 4-hydroxybenzoate. These engineered strains will be useful platforms to discover, characterize, and optimize pathways for conversions of lignin-derived aromatics. IMPORTANCE Lignin is a challenging substrate for microbial catabolism due to its polymeric and heterogeneous chemical structure. Therefore, engineering microbes for improved catabolism of lignin-derived aromatic compounds will require the assembly of an entire network of catabolic reactions, including pathways from genetically intractable strains. Constructing defined pathways for aromatic compound degradation in a model host would allow rapid identification, characterization, and optimization of novel pathways. We constructed and optimized one such pathway in E. coli to enable catabolism of a model aromatic compound, protocatechuate, and then extended the pathway to a related compound, 4-hydroxybenzoate. This optimized strain can now be used as the basis for the characterization of novel pathways.
Texture decomposition by harmonics extraction from higher order statistics

In this paper, a method of harmonics extraction from Higher Order Statistics (HOS) is developed for texture decomposition. We show that the diagonal slice of the fourth-order cumulants is proportional to the autocorrelation of a related noiseless sinusoidal signal with identical frequencies. We propose to use this fourth-order cumulants slice to estimate a power spectrum from which the harmonic frequencies can be easily extracted. Hence, a texture can be decomposed into deterministic components and indeterministic components as in a unified texture model through a Wold-like decomposition procedure. The simulation and experimental results demonstrated that this method is effective for texture decomposition and it performs better than traditional lower order statistics based decomposition methods.
Family members’ and best friend’s smoking influence on adolescent smoking differs between Eastern Finland and Russian Karelia

Aims: The aim of this study was to find out whether family members’ (mother, father, siblings) and best friend’s smoking is related to 9th grade pupils’ daily smoking in Eastern Finland and in the Pitkäranta district, in the Republic of Karelia, Russia, and whether these relations have changed in these two culturally very different neighbourhood countries from 1995 to 2013. Methods: Data comprised four cross-sectional studies in all schools of the Pitkäranta region and selected schools in Eastern Finland. In data analyses, structural equation modelling techniques were used. Results: Our findings showed that best friend’s smoking had the strongest influence on adolescents’ smoking in both countries and study years (p < .01). The relations among family members were highly different between Pitkäranta and Eastern Finland, particularly in 2013. Conclusions: Results suggest that health promotion in schools should take into account cultural impacts and pay special attention to pupils who have family members and friends who smoke.
Self-supervised learning of depth-based navigation affordances from haptic cues

This paper presents a ground vehicle capable of exploiting haptic cues to learn navigation affordances from depth cues. A simple pan-tilt telescopic antenna and a Kinect sensor, both fitted to the robot's body frame, provide the required haptic and depth sensory feedback, respectively. With the antenna, the robot determines whether an object is traversable by the robot. Then, the interaction outcome is associated to the object's depth-based descriptor. Later on, the robot to predict if a newly observed object is traversable just by inspecting its depth-based appearance uses this acquired knowledge. A set of field trials show the ability of the to robot progressively learn which elements of the environment are traversable.
Long-Term Survival of Campylobacter jejuni at Low Temperatures Is Dependent on Polynucleotide Phosphorylase Activity

ABSTRACT Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide. Infection generally occurs after ingestion of contaminated poultry products, usually conserved at low temperatures. The mechanisms promoting survival of C. jejuni in the cold remain poorly understood despite several investigations. The present study provides insight into the survival mechanism by establishing the involvement of polynucleotide phosphorylase (PNPase), a 3′-5′ exoribonuclease with multiple biological functions in cold survival. The role of PNPase was demonstrated genetically using strains with altered pnp genes (which encode PNPase) created in C. jejuni F38011 and C. jejuni 81-76 backgrounds. Survival assays carried out at low temperatures (4 and 10°C) revealed a difference of 3 log CFU/ml between the wild-type and the pnp deletion (Δpnp) strains. This did not result from a general requirement for PNPase because survival rates of the strains were similar at higher growth temperatures (37 or 42°C). trans-Complementation with plasmid pNH04 carrying the pnp gene under the control of its natural promoter restored the cold survival phenotype to the pnp deletion strains (at 4 and 10°C) but not to the same level as the wild type. In this study we demonstrate the role of PNPase in low-temperature survival of C. jejuni and therefore attribute a novel biological function to PNPase directly related to human health.
The Danish National Youth Study 2014: Study design, population characteristics and non-response analysis

Aims: This paper aims to give a description of the Danish National Youth Study 2014 in terms of study design, study population and questionnaire content. The differences between participants and non-participants regarding socioeconomic characteristics are also described. Methods: The Danish National Youth Study 2014 was a web-based survey with data collected through self-completion questionnaires administered in the classroom. There were two questionnaires: one for students, with >250 core questions; and one for school leaders on the school environment. Data collection took place at 119 high schools and 10 vocational schools. Results: A total of 75,853 students participated (70,674 high school students and 5179 vocational school students). In the participating schools, 85% of high school students and 69% of vocational school students took part in the survey. A total of 166 school leaders responded. Among the high school students, 61% were girls, and among vocational school students, 24% were girls. The average age was 17.9 years for high school students and 20.9 years for vocational school students. Participants were more likely than non-participants to be of Danish origin and to have parents with higher educational levels and a higher disposable income. Conclusions: The Danish National Youth Study 2014 contributes to knowledge on adolescent health behaviour, health and well-being. It is unique in its size, diversity of questionnaire content, high participation rate and possibility of linkage to various national registers through the Danish Civil Registration System. The study offers great opportunities for health planning and a wide range of future research projects.
Metagenome-Derived Clones Encoding Two Novel Lactonase Family Proteins Involved in Biofilm Inhibition in Pseudomonas aeruginosa

ABSTRACT Here we report the isolation and characterization of three metagenome-derived clones that interfere with bacterial quorum sensing and degrade N-(3-oxooctanoyl)-l-homoserine lactone (3-oxo-C8-HSL). By using a traI-lacZ gene fusion, the metagenome-derived clones were identified from a soil DNA library and analyzed. The open reading frames linked to the 3-oxo-C8-HSL-degrading activities were designated bpiB01, bpiB04, and bpiB07. While the BpiB07 protein was similar to a known lactonase, no significant similarities were observed for the BpiB01 and BpiB04 proteins or the deduced amino acid sequences. High-performance liquid chromatography-mass spectrometry analyses confirmed that the identified genes encode novel lactone-hydrolyzing enzymes. The original metagenome-derived clones were expressed in Pseudomonas aeruginosa and employed in motility and biofilm assays. All clones were able to reproducibly inhibit motility in P. aeruginosa. Furthermore, these genes clearly inhibited biofilm formation in P. aeruginosa when expressed in P. aeruginosa PAO1. Thus, this is the first study in which metagenome-derived proteins have been expressed in P. aeruginosa to successfully inhibit biofilm formation.
Real-Time Chain-Structured Synchronous Dataflow: Latency and Data Memory Requirement Formal Determination

We consider in this paper a Synchronous DataFlow (SDF) scheduling problem in the context of a multimedia real-time application. A SDF is in our context a chain of non-preemptive processing tasks in charge of the treatment of multimedia data blocks, denoted tokens. SDF has been wildly used in DSP (Digital Signal Processor) design environments over the past ten years. The application relies on Source, Processing and Sink tasks. A Source task produces tokens, Processing tasks process the tokens from the source down to the sink task, and a Sink task consumes the tokens. Source, Processing and Sink tasks are executed in three different processors. As a function of the underlying hardware used to retrieve or store tokens (hard-disk or hardware machine), source and sink tasks can be periodic real-time tasks or not. Each task has an input and an output buffer filled with tokens. This model is typically used in embedded multimedia applications. We propose, in this paper, a solution to optimize both the latency of the application in charge of the treatment of the tokens and the input/output buffers used to store the tokens. We establish a necessary and sufficient condition for the feasibility of a SDF chain. We then validate our results onto a multimedia system simulator of the ST Nomadik® platform.
Image Inpainting by Patch Propagation Using Patch Sparsity

This paper introduces a novel examplar-based inpainting algorithm through investigating the sparsity of natural image patches. Two novel concepts of sparsity at the patch level are proposed for modeling the patch priority and patch representation, which are two crucial steps for patch propagation in the examplar-based inpainting approach. First, patch structure sparsity is designed to measure the confidence of a patch located at the image structure (e.g., the edge or corner) by the sparseness of its nonzero similarities to the neighboring patches. The patch with larger structure sparsity will be assigned higher priority for further inpainting. Second, it is assumed that the patch to be filled can be represented by the sparse linear combination of candidate patches under the local patch consistency constraint in a framework of sparse representation. Compared with the traditional examplar-based inpainting approach, structure sparsity enables better discrimination of structure and texture, and the patch sparse representation forces the newly inpainted regions to be sharp and consistent with the surrounding textures. Experiments on synthetic and natural images show the advantages of the proposed approach.
Probabilistic Exposure Fusion

The luminance of a natural scene is often of high dynamic range (HDR). In this paper, we propose a new scheme to handle HDR scenes by integrating locally adaptive scene detail capture and suppressing gradient reversals introduced by the local adaptation. The proposed scheme is novel for capturing an HDR scene by using a standard dynamic range (SDR) device and synthesizing an image suitable for SDR displays. In particular, we use an SDR capture device to record scene details (i.e., the visible contrasts and the scene gradients) in a series of SDR images with different exposure levels. Each SDR image responds to a fraction of the HDR and partially records scene details. With the captured SDR image series, we first calculate the image luminance levels, which maximize the visible contrasts, and then the scene gradients embedded in these images. Next, we synthesize an SDR image by using a probabilistic model that preserves the calculated image luminance levels and suppresses reversals in the image luminance gradients. The synthesized SDR image contains much more scene details than any of the captured SDR image. Moreover, the proposed scheme also functions as the tone mapping of an HDR image to the SDR image, and it is superior to both global and local tone mapping operators. This is because global operators fail to preserve visual details when the contrast ratio of a scene is large, whereas local operators often produce halos in the synthesized SDR image. The proposed scheme does not require any human interaction or parameter tuning for different scenes. Subjective evaluations have shown that it is preferred over a number of existing approaches.
Biosynthesis of Novel Pyoverdines by Domain Substitution in a Nonribosomal Peptide Synthetase of Pseudomonas aeruginosa

ABSTRACT Pyoverdine is a fluorescent nonribosomal peptide siderophore made by fluorescent pseudomonads. The Pseudomonas aeruginosa nonribosomal peptide synthetase (NRPS) PvdD contains two modules that each incorporate an l-threonine residue at the C-terminal end of pyoverdine. In an attempt to generate modified pyoverdine peptides, we substituted alternative-substrate-specifying adenylation (A) and peptide bond-catalyzing condensation (C) domains into the second module of PvdD. When just the A domain was substituted, the resulting strains produced only wild-type pyoverdine—at high levels if the introduced A domain specified threonine or at trace levels otherwise. The high levels of pyoverdine synthesis observed whenever the introduced A domain specified threonine indicated that these nonnative A domains were able to communicate effectively with the PvdD C domain. Moreover, the unexpected observation that non-threonine-specifying A domains nevertheless incorporated threonine into pyoverdine suggests that the native PvdD C domain exhibited stronger selectivity than these A domains for the incorporated amino acid substrate (i.e., misactivation of a threonine residue by the introduced A domains was more frequent than misincorporation of a nonthreonine residue by the PvdD C domain). In contrast, substitution of both the C and A domains of PvdD generated high yields of rationally modified pyoverdines in two instances, these pyoverdines having either a lysine or a serine residue in place of the terminal threonine. However, C-A domain substitution more commonly yielded a truncated peptide product, likely due to stalling of synthesis on a nonfunctional recombinant NRPS template.