Miracle-dz/UniFAR-FTUs huggingface dataset:
DOMINANT AWN INHIBITOR encodes the ALOG protein originating from gene duplication and inhibits awn elongation by suppressing cell proliferation and elongation in sorghum

The awn, a needle-like structure extending from the tip of the lemma in grass species, plays a role in environmental adaptation and fitness. In some crops, awns appear to have been eliminated during domestication. Although numerous genes involved in awn development have been identified, several dominant genes that eliminate awns are also known to exist. For example, in sorghum (Sorghum bicolor), the dominant awn inhibiting gene has been known since 1921; however, its molecular features remain uncharacterized. In this study, we conducted quantitative trait locus analysis and a genome-wide association study of awn-related traits in sorghum and identified DOMINANT AWN INHIBITOR (DAI), which encodes the ALOG family protein on chromosome 3. DAI appeared to be present in most awnless sorghum cultivars, likely because of its effectiveness. Detailed analysis of the ALOG protein family in cereals revealed that DAI originated from duplication of its twin paralog (DAIori) on chromosome 10. Observations of immature awns in near-isogenic lines revealed that DAI inhibits awn elongation by suppressing both cell proliferation and elongation. We also found that only DAI gained a novel function to inhibit awn elongation through an awn-specific expression pattern distinct from that of DAIori. Interestingly, heterologous expression of DAI with its own promoter in rice inhibited awn elongation in the awned cultivar Kasalath. We found that DAI originated from gene duplication, providing an interesting example of gain-of-function that occurs only in sorghum but shares its functionality with rice and sorghum.
Changing Notions of “Ideal” Monkhood: A Case Study from a Satra of Majuli

All religions were initially founded with the aim of purifying men and women and helping them to lead ethical lives through prayers. However, unfortunately religion evolved to be a force reinforcing discrimination diverted from the original motive of religion. The Vaishnavite faith initiated by the 15th century Guru, Mahapurush Srimanta Shankardeva, in the region of Assam was also based on egalitarian and humanitarian values. The ideal monkhood in a Satra is to lead a life devoid of material pleasures and offer oneself to God by taking his name all the time. The various sects that have emerged after the demise of the Guru are the proofs that the main objective of an egalitarian society has collapsed. The social hierarchy is still maintained even if the Satras claim to have removed it. The unique culture of the Satra is its liturgical training, which includes dance, drama, music and poetry. The performing art forms were specifically for the monks to be performed only within the premises of the Satra, which are now staged and have become the sources of income for many monks. The monks are more known as performing artists than religious beings or leaders. The media is a socializing agent in itself, which has changed the concept of monkhood. Ideal monkhood is still a question, and this article is an academic effort to throw light on this question. It would try to bring forth the different ways as to how the ideologies of Neo-Vaishnavism has changed with time and how the monks have adjusted to the transformation. The findings are based on both primary and secondary data using ethnographic approach.
Cosmos white rot: First characterization, physiology, host range, disease resistance, and chemical control

A new disease of Cosmos sulphureus Cav. causing external and internal stem discoloration, premature death, and wilting was observed in 27.8% of plants with an average disease severity rating of 4.4 in Gazipur, Bangladesh. Morphological, pathological, and molecular analyses identified the isolated fungus as Sclerotinia sclerotiorum (Lib) de Bary, the causative agent of white rot disease. The optimum growth and sclerotium formation of S. sclerotiorum occurred at 20°C and pH 5.0, while glucose, peptone, yeast extract, casein, and ascorbic acid were the appropriate nutrient sources. Furthermore, mycelial growth and sclerotial development were favored in media containing potassium, magnesium, calcium, and sodium. As many as 20 plant species of 10 families; Calendula officinalisi, Chrysanthemum indicum, Catharanthus roseus, Solanum tuberosum, S. lycopersicum, S. melongena, Capsicum annum, Lablab purpureus, Phaseolus vulgari, Lens culinaris, Vigna radiata, Vigna mungo, Daucus carota, Raphanus sativus, Brassica juncea, Punica granatum, Spinacia oleracea, Ipomoea batatas, Ipomoea aquatica, and Elaeocarpus serratus were identified as the new hosts of the pathogen in Bangladesh. None of the C. sulphureus and Cosmos bipinnatus germplasms screened were genetically resistant to the pathogen. Among the tested fungicides, Autostin 50 WDG (carbendazim) and Rovral (Dicarboxamide) were most inhibitory to the fungus, while Autostin 50 WDG provided an efficient control of the pathogen in vivo up to 15 days after spray. The acquired results on characterization, physiology, host range, resistance, and fungicidal control of the pathogen could be valuable for effectively managing cosmos white rot in the field.
Feasibility of Raman spectroscopy as a potential in vivo tool to screen for pre‐diabetes and diabetes

In this article, we investigated the feasibility of using Raman spectroscopy and multivariate analysis method to noninvasively screen for prediabetes and diabetes in vivo. Raman measurements were performed on the skin from 56 patients with diabetes, 19 prediabetic patients and 32 healthy volunteers. These spectra were collected along with reference values provided by the standard glycated hemoglobin (HbA1c) assay. A multiclass principal component analysis and support vector machine (PCA‐SVM) model was created from the labeled Raman spectra and was validated through a two‐layer cross‐validation scheme. Classification accuracy of the model was 94.3% with an area under the receiver operating characteristic curve AUC of 0.76 (0.65–0.84) for the prediabetic group, 0.86 (0.71–0.93) for the diabetic group and 0.97(0.93–0.99) for the control group. Our results suggest the feasibility of using Raman spectroscopy for the classification of prediabetes and diabetes in vivo.
Treatment of giant cell tumors of the distal radius: A long‐term patient‐reported outcomes study

The distal radius is a common location for giant cell tumors (GCTs) of bone. Management includes intralesional curettage or wide excision, however, long‐term comparisons of treatment options are limited. The purpose of the current study was to evaluate our institutions' outcomes of treatment of these tumors.
Effect of Doping on Rutile TiO2 Surface Stability and Crystal Shapes

Abstract Transition‐metal‐(TM‐)doped TiO2 has been considered as promising electrode material for the oxygen evolution reaction (OER). OER activity is expected to depend on the coordination of the surface atoms. In this study, we theoretically investigate the stability of low‐index surfaces of TM‐doped rutile, (110), (100), (101) and (001), with 50 % of the Ti atoms substituted by Sc, Y, V, Nb or Ta. For Sc and Y, we also consider models with O vacancies providing the most stable oxidation state of Sc and Y. Surface energies are calculated with DFT(+U). Based on the Gibbs‐Wulff theorem, the shape of the single crystals is predicted. It is observed that p‐doping leads to spontaneous oxygen loss and O vacancies cause surface reconstruction. The Wulff shapes of n‐doped TiO2 have smaller contributions of the (110) facet and, for Nb and Ta, larger contributions of other facets. Given the higher coordinative unsaturation of the TM atoms in the latter, a higher catalytic activity is expected.
Christianity-science compatibility beliefs increase nonreligious individuals’ perceptions of Christians’ intelligence and scientific ability

Nonreligious individuals stereotype Christians as unscientific and see Christianity and science as conflicting. The present studies examined how perceptions of incompatibility between Christianity and science influence nonreligious individuals’ stereotypes of Christians in science in the US context. We measured (Study 1) and manipulated (Study 2) participants’ beliefs about the compatibility or incompatibility of Christianity and science. In Study 1 (N = 365), nonreligious participants (n = 214), more so than Christian participants (n = 151), perceived Christianity and science as incompatible, which in turn predicted perceptions of Christians as less intelligent and less scientifically able. In Study 2 (N = 799; 520 Christians, 279 nonreligious), manipulating perceived Christianity-science compatibility reduced negative perceptions of Christians’ scientific ability and general intellect among nonreligious participants. Implications for mitigating negative stereotypes of Christians in science, increasing Christians’ representation in scientific fields, and improving relations between Christians and nonreligious groups are discussed.
Major sex differences in allele frequencies for X chromosomal variants in both the 1000 Genomes Project and gnomAD

An unexpectedly high proportion of SNPs on the X chromosome in the 1000 Genomes Project phase 3 data were identified with significant sex differences in minor allele frequencies (sdMAF). sdMAF persisted for many of these SNPs in the recently released high coverage whole genome sequence of the 1000 Genomes Project that was aligned to GRCh38, and it was consistent between the five super-populations. Among the 245,825 common (MAF>5%) biallelic X-chromosomal SNPs in the phase 3 data presumed to be of high quality, 2,039 have genome-wide significant sdMAF (p-value <5e-8). sdMAF varied by location: non-pseudo-autosomal region (NPR) = 0.83%, pseudo-autosomal regions (PAR1) = 0.29%, PAR2 = 13.1%, and X-transposed region (XTR)/PAR3 = 0.85% of SNPs had sdMAF, and they were clustered at the NPR-PAR boundaries, among others. sdMAF at the NPR-PAR boundaries are biologically expected due to sex-linkage, but have generally been ignored in association studies. For comparison, similar analyses found only 6, 1 and 0 SNPs with significant sdMAF on chromosomes 1, 7 and 22, respectively. Similar sdMAF results for the X chromosome were obtained from the high coverage whole genome sequence data from gnomAD V 3.1. 2 for both the non-Finnish European and African/African American samples. Future X chromosome analyses need to take sdMAF into account.
Development and Hardware Implementation of a Phasor Measurement Unit using Microcontroller

As the world continues to move towards a Smarter Grid day by day, it has become the necessity to incorporate real-time monitoring of the grid wherein the instantaneous snapshot of the health of the grid can be made available. No other parameters than the Instantaneous Phasors, considered to be the heart-beats of the Electrical Grid, can represent the complete health status of the grid. This paper discusses how an Open Hardware Platform (Arduino Due with ARM Cortex M3 Micro-controller) can be used to estimate the phasors of a three phase system in real-time. The Pulse Per Second(PPS) signal from a GPS module is used to generate the sampling pulses. These pulses synchronise the sampling process by the Analog to Digital Converters(ADC), used by the PMU throughout the globe because of the high accuracy of the atomic clocks in the GPS satellites. The microcontroller uses a 64-Point DFT algorithm to estimate the phasors. The reference time is obtained from the GPS module which is the UTC time, with which the phasors are time stamped and displayed in a real-time Graphical User Interface(GUI) designed using Python(another open source programming language)
A Computational Biomarker of Photosensitive Epilepsy from Interictal EEG

Abstract People with photosensitive epilepsy (PSE) are prone to seizures elicited by visual stimuli. The possibility of inducing epileptiform activity in a reliable way makes PSE a useful model to understand epilepsy, with potential applications for the development of new diagnostic methods and new treatments for epilepsy. A relationship has been demonstrated between PSE and both occipital and more widespread cortical hyperexcitability using various types of stimulation. Here we aimed to test whether hyperexcitability could be inferred from resting interictal electroencephalographic (EEG) data without stimulation. We considered a cohort of 46 individuals with idiopathic generalized epilepsy who underwent EEG during intermittent photic stimulation: 26 had a photoparoxysmal response (PPR), the PPR group, and 20 did not, the non-PPR group. For each individual, we computed functional networks from the resting EEG data before stimulation. We then placed a computer model of ictogenicity into the networks and simulated the propensity of the network to generate seizures in silico [the brain network ictogenicity (BNI)]. Furthermore, we computed the node ictogenicity (NI), a measure of how much each brain region contributes to the overall ictogenic propensity. We used the BNI and NI as proxies for testing widespread and occipital hyperexcitability, respectively. We found that the BNI was not higher in the PPR group relative to the non-PPR group. However, we observed that the (right) occipital NI was significantly higher in the PPR group relative to the non-PPR group. Other regions did not have significant differences in NI values between groups.
Diagnosis and Management of Post Traumatic Recurrent Unilateral Accommodative Spasm—A Case Report

ABSTRACT Spasm of accommodation refers to constant contraction of the ciliary muscles of the eye, which fail to relax. Neurological issues, head injury, and psychogenic factors can lead to spasm of accommodation, which is generally bilateral. This case report describes the clinical presentation of traumatic, unilateral accommodative spasm in an army person. A 26-year-old male presented with complaints of diminution of near vision in the left eye noticed accidentally. History revealed a blunt injury in the eyebrow region of the left eye two months back. His best corrected visual acuity was 6/6, 0.8 M (N6) in the right eye and 6/9, 2.5 M (N18) in the left eye. Accommodative response was documented using an open-field autorefractometer that showed asymmetry in the accommodation response and pupillary diameter between the two eyes. One percent Atropine sulfate eye ointment – twice a day for 3 days – was prescribed. On the fourth day, the spasm was resolved in the left eye. Pre- and post-Atropine administration, lens thickness measurements were documented, which showed significant changes. Accommodative facility exercise was initiated after the pharmacological management. Unilateral accommodative spasm is rare and needs careful investigations. Objective assessment of accommodative response and lens thickness measurement play a vital role in confirming the diagnosis.
Did the UK Digital Design and Technology (DD&T) programme lead to innovative curriculum change within secondary schools?

Design and technology (D&T) requires teachers to continually update their knowledge and skills, with regard to new technologies, appropriate to the needs of the time (Design and Technology Association, 2011). In 2011, Ofsted identified the need for “England to keep pace with global technological change” (Ofsted 2011, p.5), in the report ‘Meeting technological challenges, a survey of schools from 2007-2010’. Following the report, the UK government funded a national programme called Digital Design and Technology (DD&T). The programme set up a network of regional support centres to provide up-to-date Professional Development (PD) courses on modern D&T subject knowledge.
MIO: microRNA target analysis system for immuno-oncology

Abstract Summary MicroRNAs have been shown to be able to modulate the tumor microenvironment and the immune response and hence could be interesting biomarkers and therapeutic targets in immuno-oncology; however, dedicated analysis tools are missing. Here, we present a user-friendly web platform MIO and a Python toolkit miopy integrating various methods for visualization and analysis of provided or custom bulk microRNA and gene expression data. We include regularized regression and survival analysis and provide information of 40 microRNA target prediction tools as well as a collection of curated immune related gene and microRNA signatures and processed TCGA data including estimations of infiltrated immune cells and the immunophenoscore. The integration of several machine learning methods enables the selection of prognostic and predictive microRNAs and gene interaction network biomarkers. Availability and implementation https://mio.icbi. at, https://github.com/icbi-lab/mio and https://github.com/icbi-lab/miopy. Supplementary information Supplementary data are available at Bioinformatics online.
A field guide to designing a health communication strategy. a resource for health communication professionals.

The purpose of this strategic communication Field Guide is to provide practical guidance to those who are in a position to design implement or support a strategic health communication effort. The emphasis of the guide is on developing a comprehensive long-term approach to health communication that responds appropriately to audience needs. The guide is based on the idea that effective strategic communication is based on the convergence of "senders" and "receivers" in which the differences between the two begin to disappear. It is also based on the recognition that communication to be effective must not be treated as a "spare" wheel used only when the efforts start to falter or fail but as a "steering" wheel that can serve as a basis for making informed choices. Strategic communication is collaborative and participatory in nature follows a sound decision-making process based on science and creates sustainable efforts that improve health outcomes. (excerpt)
Current Issues in Highway–Rail Grade Crossing Hazard-Ranking and Project Development

This paper reports on a comprehensive research study of hazard-ranking models for prioritization and selection of highway–rail grade crossing improvement projects. The U.S. Department of Transportation (DOT) accident prediction model is the most commonly used hazard-ranking model, although 11 states use state-specific models. Key variables not included in the U.S. DOT model but included in state-specific models may provide useful insight for identifying hazardous crossing locations. Emerging issues, such as the crash modeling approach used by the U.S. DOT model and the application of economic analysis principles in project prioritization, are also discussed. The findings reported in this paper provide a starting point for a broader discussion among all stakeholders in the grade crossing safety community about ways to improve hazard-ranking and project selection for highway–rail grade crossing investments.
Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli

Abstract The cell-wall recycling process is important for bacterial survival in nutrient-limited conditions and, in certain cases, is directly involved in antibiotic resistance. In the sophisticated cell-wall recycling process in Escherichia coli, the transcriptional repressor MurR controls the expression of murP and murQ, which are involved in transporting and metabolizing N-acetylmuramic acid (MurNAc), generating N-acetylmuramic acid-6-phosphate (MurNAc-6-P) and N-acetylglucosamine-6-phosphate (GlcNAc-6-P). Here, we report that both MurNAc-6-P and GlcNAc-6-P can bind to MurR and weaken the DNA binding ability of MurR. Structural characterizations of MurR in complex with MurNAc-6-P or GlcNAc-6-P as well as in the apo form revealed the detailed ligand recognition chemistries. Further studies showed that only MurNAc-6-P, but not GlcNAc-6-P, is capable of derepressing the expression of murQP controlled by MurR in cells and clarified the substrate specificity through the identification of key residues responsible for ligand binding in the complex structures. In summary, this study deciphered the molecular mechanism of the cell wall recycling process regulated by MurR in E. coli.
GeCoViz: genomic context visualisation of prokaryotic genes from a functional and evolutionary perspective

Abstract Synteny conservation analysis is a well-established methodology to investigate the potential functional role of unknown prokaryotic genes. However, bioinformatic tools to reconstruct and visualise genomic contexts usually depend on slow computations, are restricted to narrow taxonomic ranges, and/or do not allow for the functional and interactive exploration of neighbouring genes across different species. Here, we present GeCoViz, an online resource built upon 12 221 reference prokaryotic genomes that provides fast and interactive visualisation of custom genomic regions anchored by any target gene, which can be sought by either name, orthologous group (KEGGs, eggNOGs), protein domain (PFAM) or sequence. To facilitate functional and evolutionary interpretation, GeCoViz allows to customise the taxonomic scope of each analysis and provides comprehensive annotations of the neighbouring genes. Interactive visualisation options include, among others, the scaled representations of gene lengths and genomic distances, and on the fly calculation of synteny conservation of neighbouring genes, which can be highlighted based on custom thresholds. The resulting plots can be downloaded as high-quality images for publishing purposes. Overall, GeCoViz offers an easy-to-use, comprehensive, fast and interactive web-based tool for investigating the genomic context of prokaryotic genes, and is freely available at https://gecoviz.cgmlab. org
Analysis of COVID-19-associated rhino-orbital-cerebral mucormycosis patients in a tertiary care center in Northern India

Purpose: An unprecedented surge has been noted in rhino-orbital-Cerebral mucormycosis (ROCM) in times of current COVID-19 pandemic. The present prospective study aims to evaluate clinico-epidemiological profile, risk factors, management, and outcome of the cases of ROCM that presented to our tertiary care center during the study period from April to June 2021. Methods: All patients were subjected to complete history taking, ophthalmological examination, and imaging studies. The patients were staged and were treated with intravenous liposomal amphotericin B (AMB) and sino-nasal debridement of local necrotic tissue. Transcutaneous retrobulbar AMB (TRAMB), orbital decompression, and exenteration were instituted as indicated. All patients were followed up for a minimum of 6 months before arriving at the final outcome. Statistical analysis was performed. Results: A total of 49 patients presented during the study period, with a mean age of 42.2 years. The major risk factors included uncontrolled diabetes (89.8%), COVID-19 positivity (51.02%), and concurrent steroid use (38.77%). The most common presenting symptom was facial pain/swelling (43.65%), while the most common presenting sign was deterioration in vision (75.51%). Intravenous liposomal AMB was given to all patients along with sino-nasal debridement (85.71%), TRAMB (57.14%), orbital decompression (14.28%), and exenteration (12.24%). Overall, mortality at 6 months was 22.45% (11 patients). Age more than 60 years, intracranial extension, and HbA1c of more than 8.0% were observed to be statistically significant indicators of mortality. Conclusion: Early suspicion and timely diagnosis of mucormycosis at rhino-orbital stage is warranted in order to salvage life as well as visual function. TRAMB may prove as potentially favorable treatment modality in cases with limited orbital involvement.