Miracle-dz/UniFAR-FTUs huggingface dataset:
Protective Transfer: Maternal passive immunization with a rotavirus-neutralizing dimeric IgA protects against rotavirus disease in suckling neonates

Breast milk secretory IgA antibodies provide a first line of defense against enteric infections. Despite this and an effective vaccine, human rotaviruses (RVs) remain the leading cause of severe infectious diarrhea in children in low- and middle-income countries (LMIC) where vaccine efficacy is lower than that of developed nations. Therapeutic strategies that deliver potently neutralizing antibodies into milk could provide protection against enteric pathogens such as RVs. We developed a murine model of maternal protective-transfer using systemic administration of a dimeric IgA (dIgA) monoclonal antibody. We confirmed that systemically-administered dIgA passively transferred into milk and stomach of suckling pups in a dose-dependent manner. We then demonstrated that systemic administration of an engineered potent RV-neutralizing dIgA (mAb41) in lactating dams protected suckling pups from RV-induced diarrhea. This maternal protective-transfer immunization platform could be an effective strategy to improve infant mortality against enteric infections, particularly in LMIC with high rates of breastfeeding. GRAPHICAL ABSTRACT
Transcriptional response of the calcification and stress response toolkits in an octocoral under heat and pH stress

Up to one-third of all described marine species inhabit coral reefs, but the future of these hyperdiverse ecosystems is insecure due to local and global threats, such as overfishing, eutrophication, ocean warming, and acidification. Although these impacts are expected to have a net detrimental effect on reefs, it has been shown that some organisms like octocorals may remain unaffected, or benefit from, anthropogenically induced environmental change, and may replace stony corals in future reefs. Despite their potential importance in future shallow-water coastal environments, the molecular mechanisms leading to the resilience to anthropogenic-induced stress observed in octocorals remain unknown. Here, we use manipulative experiments, proteomics, and transcriptomics to show that the molecular toolkit used by Pinnigorgia flava, a common Indo-Pacific gorgonian octocoral, to deposit its calcium-carbonate skeleton is resilient to heat and seawater acidification stress. Sublethal heat stress triggered a stress response in P. flava but did not affect the expression of 27 transcripts encoding Skeletal Organic Matrix (SOM) proteins. Exposure to seawater acidification did not cause a stress response but triggered the downregulation of many transcripts, including an osteonidogen homolog present in the SOM. The observed transcriptional decoupling of the skeletogenic and stress-response toolkits provides insights into the mechanisms of resilience to anthropogenically-driven environmental change observed in octocorals.
The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive‐strand RNA viruses

Genome‐wide screens are powerful approaches to unravel regulators of viral infections. Here, a CRISPR screen identifies the RNA helicase DDX42 as an intrinsic antiviral inhibitor of HIV‐1. Depletion of endogenous DDX42 increases HIV‐1 DNA accumulation and infection in cell lines and primary cells. DDX42 overexpression inhibits HIV‐1 infection, whereas expression of a dominant‐negative mutant increases infection. Importantly, DDX42 also restricts LINE‐1 retrotransposition and infection with other retroviruses and positive‐strand RNA viruses, including CHIKV and SARS‐CoV‐2. However, DDX42 does not impact the replication of several negative‐strand RNA viruses, arguing against an unspecific effect on target cells, which is confirmed by RNA‐seq analysis. Proximity ligation assays show DDX42 in the vicinity of viral elements, and cross‐linking RNA immunoprecipitation confirms a specific interaction of DDX42 with RNAs from sensitive viruses. Moreover, recombinant DDX42 inhibits HIV‐1 reverse transcription in vitro. Together, our data strongly suggest a direct mode of action of DDX42 on viral ribonucleoprotein complexes. Our results identify DDX42 as an intrinsic viral inhibitor, opening new perspectives to target the life cycle of numerous RNA viruses.
Virtual mouse brain histology from multi-contrast MRI via deep learning

1H MRI maps brain structure and function non-invasively through versatile contrasts that exploit inhomogeneity in tissue micro-environments. Inferring histopathological information from MRI findings, however, remains challenging due to absence of direct links between MRI signals and cellular structures. Here, we show that deep convolutional neural networks, developed using co-registered multi-contrast MRI and histological data of the mouse brain, can estimate histological staining intensity directly from MRI signals at each pixel. The results provide three-dimensional maps of axons and myelin with tissue contrasts that closely mimics target histology and enhanced sensitivity and specificity compared to conventional MRI markers. Furthermore, the relative contribution of each MRI contrast within the networks can be used to optimize multi-contrast MRI acquisition. We anticipate our method to be a starting point for translation of MRI results into easy-to-understand virtual histology for neurobiologists and provide resources for validating novel MRI techniques.
Prevalence and Predictors of Left Ventricular Diastolic Dysfunction in Malaysian Patients With Type 2 Diabetes Mellitus Without Prior Known Cardiovascular Disease

Objective: Existing data showed that left ventricular diastolic dysfunction is common in individuals with type 2 diabetes mellitus (T2DM). However, most of the studies included diabetic patients who have prior cardiovascular disease, which might be the compounding factor for ventricular dysfunction. This study aimed to determine the prevalence and predictors of left ventricular diastolic dysfunction in an Asian population with T2DM without prior cardiovascular disease using the latest recommended echocardiographic assessment for left ventricular diastolic dysfunction. Design and Participants: This is a cross-sectional study in which eligible patients with T2DM without history of coronary artery disease, heart failure, or valvular heart disease were recruited. Demographic data, diabetic control, comorbidities, microvascular/macrovascular complications, and medications prescribed were recorded. Venous blood was sent to test for B-type natriuretic peptide, and transthoracic echocardiography was performed to assess left ventricular dysfunction. Setting: This study was performed in a tertiary healthcare center located in Kuala Lumpur, Malaysia. Results: Of the 301 patients, 83.1% have had T2DM for >10 years, with 45.8% being poorly controlled. Comorbidities include hypertension (77.1%), hyperlipidemia (91.0%), and pre-obesity/obesity (72.9%). Majority had absence of microvascular (albuminuria, retinopathy, and neuropathy) and macrovascular (peripheral vascular disease and stroke) complications. None had raised B-type natriuretic peptide levels, and 93.7% had no symptoms of heart failure. On echocardiographic assessment, 70.1% had left ventricular diastolic dysfunction, and 90.5% had Grade 1/mild severity. Age, ethnicity, insulin therapy, presence of hypertension, and hyperlipidemia were significantly associated with left ventricular diastolic dysfunction. Older T2DM patients of Chinese ethnicity and on insulin are about two times more likely to develop left ventricular diastolic dysfunction. Conclusion: There was a high prevalence of asymptomatic left ventricular diastolic dysfunction among patients with T2DM without prior known cardiovascular disease. Older age, insulin therapy, and Chinese ethnicity were risk factors for left ventricular diastolic dysfunction in T2DM.
An ERP Study on Attraction Effects in Advanced L2 Learners

In English, the rule of agreement is quite simple: verbs must agree with their subject head nouns in terms of number features. Despite this simplicity, agreement processing is always interrupted when the subject phrase of the sentence “The key to the cabinets is on the table,” contains two nouns with a mismatch in number features commonly known as attraction effects. This study used event-related potentials (ERPs) to examine whether late advanced second language (L2) learners can acquire native-like sensitivity of attraction effects. The results revealed that L2 learners showed ERP patterns qualitatively similar to native English speakers: ungrammatical verbs following singular attractors elicited a P600 effect relative to their grammatical counterparts, whereas this positivity was replaced by an N400 effect when plural attractors intervened between the subject head nouns and the verbs. Of particular interest, given that, compared to native speakers, the amplitude of the P600 effect elicited by L2 learners was smaller, there was a quantitative difference between native speakers and L2 learners. We proposed that these two ERP components represented the two processing routes of agreement: the P600 effect indexed a full, combinatorial process, which parsed morphosyntactic features between agreement controllers and targets, whereas the N400 effect indexed a shallow, heuristic process, which evaluated lexical associations between agreeing elements. Moreover, similar to native speakers, advanced L2 learners showed an asymmetrical pattern of attraction effects, in that plural attractors were interfered with ungrammaticality at disagreeing verbs, but they did not cause any difficulties in processing grammatical sentences at agreeing verbs. The overall results suggested that compared to native processing, L2 processing of complex agreement with attractor interference was shallower and therefore late advanced L2 learners could not achieve native-like attraction effects.
Reliability Generalization Study of the Person-Centered Care Assessment Tool

The so-called Person-Centered Care (PCC) model identifies three fundamental principles: changing the focus of attention from the disease to the person, individualizing care, and promoting empowerment. The Person-Centered Care Assessment Tool (P-CAT) has gained wide acceptance as a measure of PCC in recent years due to its brevity and simplicity, as well as its ease of application and interpretation. The objective of this study is to carry out a reliability generalization meta-analysis to estimate the internal consistency of the P-CAT and analyze possible factors that may affect it, such as the year of publication, the care context, the application method, and certain sociodemographic properties of the study sample. The mean value of α for the 25 samples of the 23 studies in the meta-analysis was 0.81 (95% CI: 0.79–0.84), with high heterogeneity (squared-I = 85.83%). The only variable that had a statistically significant relationship with the reliability coefficient was the mean age of the sample. The results show that the P-CAT gives acceptably consistent scores when its use is oriented toward the description and investigation of groups, although it may be affected by variables such as the age of participants.
Average refractive index of tendon as a function of water content

Experimental data on the dependence of the average refractive index of rat tail tendon (RTT) on water content are reported. Using optical coherence tomography, the average group refractive index (at a wavelength of 930 nm) and cross-section area of rat tail tendon fascicle specimens during their air-drying and rehydration were monitored. The dependence of the average group refractive index of RTT ( n g ) on the volume fraction of water ( C w ) has been found to be nonlinear and to be well approximated by the quadratic polynomial n g = 1.5713 – 0.1969 C w – 0.0328( C w ) 2 . The reported data are shown to be in good agreement with previously published data for bovine cornea.
Numerical and Experimental Research on Single-Valve and Multi-Valve Resonant Systems

Multiple valves in the pipeline system belong to obvious periodic structure distribution types. When a high-speed airstream flows through the pipeline valve, it produces obvious aero-acoustic and acoustic resonance. Acoustic resonant systems with single and six-pipe valves were investigated to understand the flow and acoustic characteristics using a numerical simulation method and testing method. The strongest acoustic resonance occurred at a specific flow velocity with a corresponding Strouhal number of 0.47 corresponding to the geometric parameters in the paper. Moreover, acoustic resonance occurred in a certain velocity range, rather than increasing with the increase of the velocity of the pipeline. This regular increase provided an important theoretical basis for the prediction of the acoustic resonant and ultimate acoustic load of a single-valve system. When the pipeline was attached with multiple valves and the physical dimension was large, the conventional aero-acoustics calculation results were seriously attenuated at high frequency; the calculation method involving a cut-off frequency in this paper was presented and could be used to explain the excellent agreement of the sound pressure level (SPL) below the cut-off frequency and the poor agreement above the cut-off frequency. A new method involving steady flow and stochastic noise generation and radiation (SNGR) was proposed to obtain better results for the SPL at the middle and high frequencies. The comparison results indicated that the traditional method of Lighthill analogy and unsteady flow could accurately acquire aerodynamic noise below the cut-off frequency, while the new method involving steady flow and SNGR could quickly acquire aerodynamic noise above the cut-off frequency.
Liposome as transdermal carrier for labisia pumila and ficus deltoidea water extracts

In this study, liposome has been investigated as the potential transdermal carrier for Labisia pumila (Lp) and Ficus deltoidea (Fd) . The encapsulation efficiency, zeta potential, particle size and transdermal transport properties of the herbal extracts loaded liposomes were determined to characterize the delivery system. The entrapment efficiency for Lipo- Lp and Lipo- Fd were 35.47 ± 2.71% and 31.23 ± 7.65%, respectively. The average diameter were153.35nm and 131.9nm, respectively and the zeta potential were -47.2 and -46.8, respectively. The permeability and partition coefficient (K) of Lipo- Lp were 1.31cm h -1 and 10.05, respectively and the permeability and partition coefficient (K) of Lipo- Fd were 1.28cm h -1 and 7.62, respectively. Labisia pumila and Ficus deltoidea loaded liposomes showed better transdermal permeation compared to un-encapsulated Labisia pumila and Ficus deltoidea indicative of potential actives herbal cosmetic formulations.
Surfaceome Proteomic of Glioblastoma Revealed Potential Targets for Immunotherapy

Glioblastoma (GBM) is the most common and devastating malignant brain tumor in adults. The mortality rate is very high despite different treatments. New therapeutic targets are therefore highly needed. Cell-surface proteins represent attractive targets due to their accessibility, their involvement in essential signaling pathways, and their dysregulated expression in cancer. Moreover, they are potential targets for CAR-based immunotherapy or mRNA vaccine strategies. In this context, we investigated the GBM-associated surfaceome by comparing it to astrocytes cell line surfaceome to identify new specific targets for GBM. For this purpose, biotinylation of cell surface proteins has been carried out in GBM and astrocytes cell lines. Biotinylated proteins were purified on streptavidin beads and analyzed by shotgun proteomics. Cell surface proteins were identified with Cell Surface Proteins Atlas (CSPA) and Gene Ontology enrichment. Among all the surface proteins identified in the different cell lines we have confirmed the expression of 66 of these in patient’s glioblastoma using spatial proteomic guided by MALDI-mass spectrometry. Moreover, 87 surface proteins overexpressed or exclusive in GBM cell lines have been identified. Among these, we found 11 specific potential targets for GBM including 5 mutated proteins such as RELL1, CYBA, EGFR, and MHC I proteins. Matching with drugs and clinical trials databases revealed that 7 proteins were druggable and under evaluation, 3 proteins have no known drug interaction yet and none of them are the mutated form of the identified proteins. Taken together, we discovered potential targets for immune therapy strategies in GBM.
Comparing Soil Nitrous Oxide and Methane Fluxes From Oil Palm Plantations and Adjacent Riparian Forests in Malaysian Borneo

Riparian forests are often kept as buffers between rivers and oil palm plantations. Many benefits of riparian forests, such as increasing biodiversity and providing a travel corridor for wildlife have been documented. Conversely, data on fluxes of the greenhouse gases nitrous oxide (N2O) and methane (CH4) from riparian forests are sparse. Nitrogen (N) from fertilizer applied in the oil palm plantations leached to the adjacent riparian forests, may increase emissions of N2O. Methane (CH4) fluxes might also differ between oil palm plantations and riparian forests due to carbon (C) availability. In this scoping study, we installed transects from three mature oil palm plantations to adjacent riparian forests within the SAFE project landscape in Sabah, Malaysia (https://www.safeproject.net) for measurements of greenhouse gases and associated parameters every 2 months for 13 months. Emissions of N2O were higher from riparian forests with 40.4 [95% confidence intervals (CI): 35.7–44.6] μg N2O-N m–2 h–1 than from an equivalent area of oil palm plantation 27.6 (CI: 23.1–32.3) μg N2O-N m–2 h–1. Methane uptake was significantly higher from the riparian forest with −14.7 (CI: −21.1 to −8.3) μg CH4-C m–2 h–1 compared to slight positive emission in the oil palm plantations of 6.3 (CI: 1.1–11.4) μg CH4-C m–2 h–1. We are contributing urgently needed flux data for less well studied riparian forests in the Tropics, however, additional long-term studies are needed to be able to draw wider conclusions than possible from this scoping study alone.
Time Is Money: The Effect of Mode-of-Thought on Financial Decision-Making

The dual-system approach holds that deliberative decisions and in-depth evaluation processes lead people to better financial decisions. However, research identifies situations where optimal economic decisions may stem from a more intuitive decision process. In the current work, we present three experimental studies that examined how these two modes-of-thought affect financial decisions. In Study 1, deliberative processes were indeed associated with better one-shot descriptive-based financial decisions. However, Study 2 showed that when participants were asked to make repeated decisions and were required to learn from their experience, the advantage of deliberative over intuitive processes was eliminated. In addition, when participants employed intuitive processes, the quality of their financial decisions improved significantly with experience. Finally, Study 3 showed that the deliberative processing style may lose its advantage when information is not fully available. Overall, these findings suggest that deliberation may contribute to financial decision-making in one-shot decisions. However, when information is lacking, and decisions are repetitive, intuitive processes might be just as good.
Efficacy of Sertraline Plus Placebo or Add-On Celecoxib in Major Depressive Disorder: Macrophage Migration Inhibitory Factor as a Promising Biomarker for Remission After Sertraline—Results From a Randomized Controlled Clinical Trial

Introduction: Previous research delivers strong indications that inflammatory activation leads to treatment resistance in a subgroup of patients with Major Depressive Disorder (MDD). Thus, tailored interventions are needed. The present study aimed to find potential biomarkers that may enable patients to be stratified according to immune activation. Methods: A phase IIa randomized placebo-controlled trial was performed to assess levels of inflammatory compounds in responders/remitters and non-responders/non-remitters to sertraline plus celecoxib (n = 20) and sertraline plus placebo (n = 23). Levels of macrophage migration inhibitory factor, neopterin, and tumor necrosis factor alpha were determined by enzyme-linked immunosorbent assay; response and remission were measured by reduction of the Montgomery Åsberg Depression Rating Scale score. Results: Both treatment groups showed a significant decline in depression symptoms, but no difference was found between groups. A clear pattern emerged only for macrophage migration inhibitory factor: placebo remitters showed significantly lower baseline levels than non-remitters (a similar trend was seen in responders and non-responders) while celecoxib responders showed a trend for higher baseline levels than non-responders. Conclusion: Small subsample sizes are a notable limitation, wherefore results are preliminary. However, the present study provides novel insights by suggesting macrophage migration inhibitory factor as a promising biomarker for treatment choice. The trial was registered in EU Clinical Trials Register (EU-CTR): https://www.clinicaltrialsregister. eu/ctr-search/trial/2009-011990-34/DE, EudraCT-No.: 2009-011990-34.
Magnetically Induced Temporal Interference for Focal and Deep-Brain Stimulation

Transcranial magnetic stimulation (TMS) is a non-invasive brain stimulation technique that has been clinically applied for neural modulation. Conventional TMS systems are restricted by the trade-off between depth penetration and the focality of the induced electric field. In this study, we integrated the concept of temporal interference (TI) stimulation, which has been demonstrated as a non-invasive deep-brain stimulation method, with magnetic stimulation in a four-coil configuration. The attenuation depth and spread of the electric field were obtained by performing numerical simulation. Consequently, the proposed temporally interfered magnetic stimulation scheme was demonstrated to be capable of stimulating deeper regions of the brain model while maintaining a relatively narrow spread of the electric field, in comparison to conventional TMS systems. These results demonstrate that TI magnetic stimulation could be a potential candidate to recruit brain regions underneath the cortex. Additionally, by controlling the geometry of the coil array, an analogous relationship between the field depth and focality was observed, in the case of the newly proposed method. The major limitations of the methods, however, would be the considerable intensity and frequency of the input current, followed by the frustration in the thermal management of the hardware.
Simulation Evaluation for Methods Used to Determine Muscular Internal Force Based on Joint Stiffness Using Muscular Internal Force Feedforward Controller for Musculoskeletal System

This study proposes two novel methods for determining the muscular internal force (MIF) based on joint stiffness, using an MIF feedforward controller for the musculoskeletal system. The controller was developed in a previous study, where we found that it could be applied to achieve any desired end-point position without the use of sensors, by providing the MIF as a feedforward input to individual muscles. However, achieving motion with good response and low stiffness using the system, posed a challenge. Furthermore, the controller was subject to an ill-posed problem, where the input could not be uniquely determined. We propose two methods to improve the control performance of this controller. The first method involves determining a MIF that can independently control the response and stiffness at a desired position, and the second method involves the definition of an arbitrary vector that describes the stiffnesses at the initial and desired positions to uniquely determine the MIF balance at each position. The numerical simulation results reported in this study demonstrate the effectiveness of both proposed methods.