Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification and Reaction Assay of Galloylated Catechins Hydrolase in Tea Plant

In this experiment,high activity of galloylated catechins hydrolase(GCH) was detected existing in tea plant(Camellia sinensis(L.) O.Kuntze) by enzymology analysis in vitro,combining thin layer chromatography(TLC),high performance liquid chromatography(HPLC),and liquid chromatography with tandem mass spectrometry(LC-MS/MS) analysis.The galloylated catechins could be hydrolyzed to ungalloylated catechins and gallic acid(GA) with the GCH action.The optimum reaction assay of GCH has been established.The 2.5 mL enzyme reaction assay included 0.1 mol/L phosphate buffer(pH 6.5),0.2 mmol/L EGCG,2.4 mmol/L sodium ascorbate,crude enzyme extract(0.1 mg total protein),and then it was incubated at 30℃ for 30 min.Besides,the crude enzyme extract was partially purified via ammonium sulfate fractionation,anion exchange chromatography on Q Sepharose Fast Flow column and gel filtration chromatography on superdex 200 sequentially.
The Forest of Dean Iron Industry 1st to 4th centuries A.D.

This thesis examines the considerable volume of new archaeological evidence revealed by field survey, aerial survey and excavation in recent years west of the Severn in Gloucestershire, along the lower Wye Valley and in south Herefordshire where it borders Gloucestershire and Monmouthshire. Without its vast iron resource of rich hematites Roman Dean would have been unremarkable. Its iron industry proved to be central to the development of high status buildings and a sophisticated communications system involving Roman military-constructed roads and its river boundaries, the Severn and the Wye. The thesis is therefore as much about Roman Dean as it is about the iron industry. Recent evidence has made possible an hypothesis concerning the administration of the industry, not merely that the resources came under state control but that the area was probably an Imperial Estate with the central woodlands reserved as an Imperial Forest for hunting. There is no longer any doubt that, unlike the Wealden iron industry, Dean ores were, moved over sometimes considerable distances to the smelting and smithing sites in and outside of Dean. So were its iron products - in the first century supplying a mainly Roman military market and during the second century a rapidly expanding civilian market. Estimates are made for iron production and manpower requirements for each of the four centuries under review and evidence is presented for a major recession in the early third century AD which resulted in the closure of some of the largest and oldest iron-working centres and from which the industry never fully recovered during the period of Roman occupation.
The clinical profile of dysthymia in a group of Latin American women

Summary More than half of all patients consulting at public health facilities in Cali, Colombia have been shown to present mental disorders, the majority of which were non-psychotic; there is a high female preponderance amongst the affective disorders. As in other developing countries, in Colombia depressive disorders tend to present as somatic complaints, rather than as mood disturbances. The nomenclature used to describe these disorders is poorly codified, with most patients classed as “neurotic” or “depressed”; the diagnosis of “dysthymia” is very seldom encountered. Mental disorders are frequently misdiagnosed in primary care settings; when identified, treatment of depression by GPs generally involves TCAs or MAOIs, sometimes in conjunction with anxiolytics or neuroleptics. Only very severe cases are referred to psychiatrists, and many patients purchase drugs upon the recommendation of a pharmacist. Diagnostic trends and treatment results of a comparative study of amisulpride and viloxazine carried out in 80 patients assessed by DSM III-R criteria are reported.
ELABORAÇÃO DE ESFIHAS DE FRANGO ADICIONADAS DE FARINHA DE CASCA DE BERINJELA: ANÁLISE FÍSICO-QUÍMICA E SENSORIAL

O estudo objetivou verificar a aceitabilidade sensorial de esfihas de frango tradicionais e adicionadas de farinha de casca de berinjela (FCB), e determinar a composicao fisico-quimica da formulacao tradicional e daquela contendo FCB com aceitacao sensorial semelhante a tradicional. Foram elaboradas 5 formulacoes de esfihas de frango, sendo: padrao – 0% (F1) e as demais adicionadas de 5,0% (F2), 10,0% (F3), 15,0% (F4) e 20,0% (F5) de FCB. Participaram da analise sensorial 130 provadores nao treinados, de ambos os generos, com idade entre 18 a 70 anos. Em geral, as formulacoes F1 e F2 demonstraram maior aceitacao sensorial pelos provadores nos atributos avaliados (aparencia, aroma, sabor, textura, cor, aceitacao global e intencao de compra). A amostra F2 apresentou maiores teores de cinzas, carboidratos e calorias, com valores inferiores de umidade e proteinas comparada a F1. A elaboracao dos produtos permitiu comprovar que um nivel de adicao de ate 5,0% de FCB em esfihas de frango foi bem aceito pelos provadores, obtendo-se uma aceitacao sensorial semelhante ao produto tradicional e com boas expectativas de comercializacao.
Assignability of numerical characteristics of time-varying systems

The main aim of this article is to survey and discuss the existing state of art concerning the assignability by a feedback of numerical characteristics of linear continuous and discrete time-varying systems. Most of the results present necessary or sufficient conditions for different formulation of the Lyapunov spectrum assignability problem. These conditions are expressed in terms of various controllability types and optimalizability of the controlled systems and certain properties of the free system such as: regularity, diagonalizability, boundness away, integral separation and reducibility.
Transforming growth factor β1 repression of the HLA-DRα gene is mediated by conserved proximal promoter elements

Transforming growth factor beta 1 (TGF-beta 1) is a pleiotropic cytokine that decreases the expression of class II MHC Ag in the melanoma cell line Hs294T(c). To investigate the mechanism of this repression, we have examined the effect of TGF-beta 1 on expression of the HLA-DR alpha gene. Both the constitutive level of HLA-DR protein and DR alpha mRNA were repressed by treatment with TGF-beta 1. The proximal 176 bp of the DR alpha promoter were sufficient to confer TGF-beta 1 repression on a reporter gene. Deletional and mutational analysis of the DR alpha promoter revealed that the conserved S and X1 promoter elements were important for basal expression of DR alpha and also mediated the down-regulation by TGF-beta 1. Mobility shift assays and in vivo footprinting showed no change in occupancy of the proximal DR alpha promoter after TGF-beta 1 treatment. These results identify the DNA elements that mediate repression of the HLA-DR alpha gene by TGF-beta 1 and suggest that TGF-beta 1 acts at these sites without causing a change in promoter occupancy.
Science Journals — AAAS

BACKGROUND: Futurehigh-performanceportable andwearable optical devices and systems with small footprints and low weights will require components with small form factors and enhanced functionality. Planar components basedondiffractive optics (e.g., gratings, Fresnel lenses) and thin-film optics (e.g., dielectric filters, Bragg reflectors) have been around for decades; however, their limited functionality and difficulty of integration have been key incentives to search for better alternatives. Owing to its potential for vertical integration and marked design flexibility, metasurface-based flat optics provides a rare opportunity to overcome these challenges. The building blocks (BBs) ofmetasurfaces are subwavelength-spaced scatterers. By suitably adjusting their shape, size, position, and orientation with high spatial resolution, one can control the basic properties of light (phase, amplitude, polarization) and thus engineer its wavefront at will. This possibility greatly expands the frontiers of optical design by enablingmultifunctional componentswithattendant reduction of thickness, size, and complexity. ADVANCES: Recent progress in fabrication techniques and in the theory and design of metasurfaces holds promise for this new optical platform (metaoptics) to replace or complement conventional components in many applications. One major advance has been the migration to all-dielectric metasurfaces. Here, we discuss the key advantages of using dielectric phase-shifting elements with low optical loss and strong light confinement in the visible and near-infrared regions as BBs of flat lenses (metalenses). High–numerical aperture metalenses that are free of spherical aberrations have been implemented to achieve diffractionlimited focusing with subwavelength resolution, without requiring the complex shapes of aspherical lenses. Achromatic metalenses at discrete wavelengths and over a bandwidth have been realized by dispersion engineering of the phase shifters. By suitably adjusting the geometrical parameters of the latter, one can impart polarizationandwavelength-dependent phases to realize multifunctional metalenses with only one ultrathin layer. For example, polarization-sensitive flat lenses for chiral imaging and circular dichroism spectroscopy with high resolution have been realized, and offaxis metalenseswith large engineered angular dispersion have been used to demonstrate miniature spectrometers. The fabrication of metalenses is straightforward and often requires one-step lithography, which can be based on high-throughput techniques such as deepultraviolet and nanoimprint lithography.
EFFICIENT DATA HIDING TECHNIQUE IN ENCRYPTED IMAGE

This work proposes a novel revocable data hiding system for encrypted image. After encrypting the entire data of an uncompressed image by a stream cipher, the additional data can be embedded into the image by modifying a small proportion of encrypted data. With ancoded image containing additional data, one may to start with decrypt it using the encryption key, and the decrypted version is similar to the original image. Allowing to the data-hiding key, with the utility of spatial association in normal image, the inserted data can be successfully extracted and the original image can be perfectly recovered
NONLINEAR STOCHASTIC HEAT EQUATIONS WITH CUBIC NONLINEARITIES AND ADDITIVE Q-REGULAR NOISE IN R

Semilinear stochastic heat equations perturbed by cubic-type nonlinearities and additive space-time noise with homogeneous boundary conditions are discussed in R1. The space-time noise is supposed to be Gaussian in time and possesses a Fourier expansion in space along the eigenfunctions of underlying Lapace operators. We follow the concept of approximate strong (classical) Fourier solutions. The existence of unique continuous L2-bounded solutions is proved. Furthermore, we present a procedure for its numerical approximation based on nonstandard methods (linear-implicit) and justify their stability and consistency. The behavior of related total energy functional turns out to be crucial in the presented analysis.
Single-cell analysis of Hodgkin and Reed-Sternberg cells: molecular heterogeneity of gene expression and p53 mutations

We have used a single-cell based polymerase chain reaction (PCR) amplification technique to examine the gene expression pattern in single Hodgkin's and Reed-Sternberg (H&RS) cells from seven patients with Hodgkin's disease. Single cells were isolated from lymph nodes obtained at diagnosis (5 of 7 patients) or in first or second relapse (2 of 7 patients). Gene expression was examined by hybridization to a panel of 22 cDNA probes. Forty-nine H&RS cells (and 23 CD3+ or CD20+ lymphocytes as controls) from four patients with nodular sclerosing Hodgkin's disease (HD) and one patient each with lymphocyte predominant and mixed-cellularity HD were successfully analyzed by PCR. This analysis provides evidence that single H&RS cells can coexpress genes characteristic of several hematopoietic lineages (monocytes and lymphocytes). Genes characteristic of activated lymphoid cells are expressed in most H&RS cells. Heterogeneity of expression for certain genes between different cases was found and may eventually define molecular subgroups of HD. These findings indicate that H&RS cells of HD resemble activated hematopoietic cells. Phenotypically similar cells from different cases exhibit characteristic molecular differences. In one patient, 5 of 7 single RS cells showed identical p53 cDNA mutations at codon 246 on specific reverse transcriptase [RT]-PCR and sequencing of exons 5 through 8. The novel experimental approach may provide a valuable tool for understanding the molecular events in newly diagnosed Hodgkin's disease and progression of the disease.
Corifollitropin-α compared to daily r-FSH in for patients undergoing intracytoplasmic sperm injection: Clinical trial study

Abstract Background The current treatment regimen for ovarian stimulation in Intracytoplasmic sperm injection (ICSI) patients is daily injections of Gonadotropins. Recombinant DNA technologies have produced a new recombinant molecule that is a long-acting Follicle Stimulating Hormone (FSH), named corifollitropin alfa. A single injection of long-acting FSH can replace seven daily FSH injections during the first week of controlled ovarian stimulation (COS) and can make assisted reproduction more patients-friendly. There is limited data with different results in this area. Objective To compare the effectiveness of long-acting FSH vs. daily r-FSH in terms of pregnancy and safety outcomes in women undergoing ICSI cycles. Materials and Methods In this clinical trial study, 109 women who were the candidates for ICSI at azzahra hospital were divided in two groups. The first group received 150 units of daily Gonal-f from second or third day of menstruation. The second group received a 150IU corifollitropin alfa on the second or third day of mensuration, and the treatment continued from day eighth of stimulation with Gonal-f based on the ultrasound finding. Both the groups received GnRH antagonist from fifth day of stimulation. Two groups were compared in terms of number of dominant follicles, number of oocytes, stimulation duration, total number of embryos, number of transferred embryos, and success rate of pregnancy. Results No significant difference was found between the two groups in terms of stimulation duration, number of follicles, number of oocytes, total number of embryos, and number of transferred embryos. Moreover, pregnancy outcomes including chemical pregnancy rate (positive pregnancy test), clinical pregnancy rate (detection of fetal heart), the rate of ovarian hyper-stimulation syndrome, multiple-pregnancy, ectopic pregnancy, and miscarriage didn't have a significant difference between the two groups. Conclusion As corifollitropin alfa was as effective as r-FSH, it could be used as an alternative to ovulation stimulation method in patients undergoing ICSI.
Objective Observer-Relative Flow Visualization in Curved Spaces for Unsteady 2D Geophysical Flows

Computing and visualizing features in fluid flow often depends on the observer, or reference frame, relative to which the input velocity field is given. A desired property of feature detectors is therefore that they are objective, meaning independent of the input reference frame. However, the standard definition of objectivity is only given for Euclidean domains and cannot be applied in curved spaces. We build on methods from mathematical physics and Riemannian geometry to generalize objectivity to curved spaces, using the powerful notion of symmetry groups as the basis for definition. From this, we develop a general mathematical framework for the objective computation of observer fields for curved spaces, relative to which other computed measures become objective. An important property of our framework is that it works intrinsically in 2D, instead of in the 3D ambient space. This enables a direct generalization of the 2D computation via optimization of observer fields in flat space to curved domains, without having to perform optimization in 3D. We specifically develop the case of unsteady 2D geophysical flows given on spheres, such as the Earth. Our observer fields in curved spaces then enable objective feature computation as well as the visualization of the time evolution of scalar and vector fields, such that the automatically computed reference frames follow moving structures like vortices in a way that makes them appear to be steady.
Deep Learning for Price Movement Prediction Using Convolutional Neural Network and Long Short-Term Memory

The prediction of stock price movement direction is significant in financial studies. In recent years, a number of deep learning models have gradually been applied for stock predictions. This paper presents a deep learning framework to predict price movement direction based on historical information in financial time series. The framework combines a convolutional neural network (CNN) for feature extraction and a long short-term memory (LSTM) network for prediction. We specifically use a three-dimensional CNN for data input in the framework, including the information on time series, technical indicators, and the correlation between stock indices. And in the three-dimensional input tensor, the technical indicators are converted into deterministic trend signals and the stock indices are ranked by Pearson product-moment correlation coefficient (PPMCC). When training, a fully connected network is used to drive the CNN to learn a feature vector, which acts as the input of concatenated LSTM. After both the CNN and the LSTM are trained well, they are finally used for prediction in the testing set. The experimental results demonstrate that the framework outperforms state-of-the-art models in predicting stock price movement direction.
Reconstruction of MADRS in Benin: a contribution to the study of its cross-cultural validity

Summary In order to evaluate the validity of the Montgomery and Asberg Depression Rating Scale (MADRS) in a Black African context, the MADRS construction study was replicated in Benin (West Africa) following the same initial procedure. Ninety-two Beninese French-speaking, major depressed out-patients were rated with the French version of the CPRS and were administered 75 mg/day clomipramine for 4 weeks. Forty-nine patients were rated at the end of the period. A list of items with a frequency higher than 70% was pre-selected from this data in strict accordance with the Montgomery and Asberg procedure; the ten items most sensitive to change Were then extracted from this list. The new scale shared eight of the ten MADRS items. Only pessimistic and suicidal thoughts, perhaps culture-bound, were absent and replaced in the new list by reported muscular tension and reduced sexual interest. None of the classic African symptoms involving somatic complaints or persecution ideas were selected by the procedure. The interest of using a shortened version of the MADRS, limited to the common eight items, is discussed.
Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14

Vascular endothelial cell (EC) injury by lipopolysaccharides (LPS) plays a major role in the pathogenesis of gram-negative bacterial sepsis and endotoxic shock. The studies described here were performed to define further the molecular mechanisms involved in the EC responses to LPS. We showed that serum was required for LPS-mediated cytotoxicity for bovine brain microvessel, pulmonary, and aortic ECs and that anti-human CD14 antibodies completely blocked LPS-mediated cytotoxicity for ECs in the presence of human serum. The addition of a recombinant soluble form of human CD14 to serum-free medium restored the LPS-mediated cytotoxicity, whereas the addition of LPS binding protein (LBP), a serum protein that potentiates LPS-induced responses to monocytes, had no effect. A similar dependency on serum or recombinant soluble CD14 (under serum-free conditions) was observed for LPS-induced secretion of interleukin-6 by human umbilical vein ECs. These findings indicate that soluble CD14 is required for LPS-mediated EC responses independently of LPB, suggesting that serum soluble CD14 represents a naturally occurring agonist for EC responses to LPS.
The Role of Insulators and Transcription Factors in Genome Organization and Function in Drosophila

The Role of Insulators and Transcription Factors in Genome Organization and Function in Drosophila By Jingping Yang Epigenetic changes can alter the genome function without altering their base composition. These differences can be inherited and can provide an important source of variation within populations that can be acted upon by natural selection. Epigenetic changes in gene expression can take place via covalent modifications of histone or DNA as well as the three-dimensional organization of chromatin in the nucleus. Insulators mediate chromatin interactions in cis or trans between different regions of the genome and may be important factors regulating the 3D organization of the genome. BEAF-32 is an insulator protein highly conserved in Drosophila but not found in other species. Here I describe an analysis of the epigenetic function of BEAF-32 in Drosophila. I identify the BEAF-32 insulator as a cis regulatory element separating genes arranged in a head-tohead orientation. I then compare the genome-wide binding landscapes of the BEAF-32 in four different Drosophila species and highlight the evolutionarily conserved presence of this protein between close adjacent genes. During the formation of new Drosophila species, binding of BEAF-32 in the genome is altered along with changes in genome organization caused by DNA re-arrangements. The alterations of BEAF-32 distribution correlate with new gene expression profiles, which in turn translate into specific and distinct phenotypes. Epigenetic information encoded in the 3D organization of the genome mediated by insulators needs to be faithfully transmitted through mitosis and meiosis in order to effect evolutionary change. To address this issue, I have also studied the function of the Myc transcription factor. I found that a subset of Myc sites remain on mitotic chromatin and overlap with aligned insulator proteins binding sites. These sites are enriched at the boundaries of topological chromosome domains, suggesting they may be important for maintaining chromosome structure throughout the cell cycle. Together, these results suggest a mechanism for the establishment of differences in transcription patterns during evolution and may help to decipher the role of epigenetic changes in evolution. The Role of Insulators and Transcription Factors in Genome Organization and Function in Drosophila By Jingping Yang M.S., Nanjing University, 2007 B.S., Nanjing University, 2004 Advisor: Victor G. Corces, Ph.D. A dissertation submitted to the Faculty of the James T. Laney School of Graduate Studies of Emory University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Graduate Division of Biological and Biomedical Sciences Population Biology, Ecology, and Evolution