Miracle-dz/UniFAR-FTUs huggingface dataset:
Impacts of the prostate stem cell antigen (PSCA) and Clostridium perfringens enterotoxin (CPE) on the apoptosis and cell cycle regulatory genes in PC3

Abstract Clostridium perfringens enterotoxin (CPE) has anti-prostate cancer effects and the prostate stem cell antigen (PSCA) has been used as a plasmid-based vaccine. So we expressed both of them in the PC3 cells to evaluate their effects on cell cycling and apoptosis. The PC3 cells were transfected either by the pBudCE4.1-CPE-PSCA or empty plasmid. The expression of the cpe and PSCA genes in transfected PC3 was evaluated. The apoptosis genes (Fas, P53, Bak, and Bax) as well as cell cycling genes (cyclin D1 and E) expression was evaluated by qPCR. Successful expression of cpe and PSCA in PC3 cells was confirmed. The flow cytometry results showed the cellular death rates of 62.6% and 21.8% for PC3 cells transformed with recombinant and empty plasmids respectively. Bak, Fas, Bax and P53 genes were significantly upregulated in PC3 cells transformed with pBudCE4.1-CPE-PSCA, while cyclin D1 and E were downregulated when compared with the pBudCE4.1-transfected PC3 and normal cells (p < .05). The results showed the lethal consequences of cpe and PSCA genes expression on PC3 transfected cells. Expression of the cpe and PSCA genes affects the PC3 cell death so it could be a suitable candidate for further researches in prostate cancer vaccine development.
Aspartate racemase and D-aspartate in starfish; possible involvement in testicular maturation

ABSTRACT D-Aspartate, aspartate racemase activity, and D-aspartate oxidase activity were detected in tissues from several types of starfish. Aspartate racemase activity in male testes of Patiria pectinifera was significantly elevated in the summer months of the breeding season compared with spring months. We also compared aspartate racemase activity with the gonad index and found that activity in individuals with a gonad index ≥6% was four-fold higher than that of individuals with a gonad index <6%. The ratio of the D-form of aspartate to total aspartate was approximately 25% in testes with a gonad index <6% and this increased to approximately 40% in testes with a gonad index ≥6%. However, such changes were not observed in female ovaries. Administration of D-aspartate into male starfish caused testicular growth. These results indicate the possible involvement of aspartate racemase and D-aspartate in testicular maturation in echinoderm starfish. Graphical abstract Changes in the ratio of D-aspartate to total aspartate and in aspartate racemase activity during maturation of the starfish testis.
Diurnal and day-to-day variations in isometric and isokinetic strength

ABSTRACT Time-of-day effects in strength performance have been extensively investigated due to their relevance in competitive sports. However, most studies use large measurement intervals making it difficult to monitor potential performance changes throughout the day. Furthermore, previous studies have exclusively focused on how the time of day affects strength on a group level and ignored the individual differences in the times of peak performance. Therefore, the main purpose of this study was to investigate the diurnal and day-to-day variations in isometric and isokinetic leg, arm and trunk strength over six different times of the day. Following a familiarization test, 19 trained males (age: 24.1 ± 2.5 years) performed isometric and isokinetic strength assessments at six different times of the day (7:00, 10:00, 13:00, 16:00, 19:00, and 21:00) with an isokinetic dynamometer. An eighth test session was performed at the same time of the day as the seventh test session to investigate the day-to-day variations and the difference between diurnal and day-to-day variations were compared. All tests were separated by at least 48 h. The start time for the first session was randomized. The mean maximum isometric leg strength was 5.85 ± 0.80 N.kg−1 and 4.99 ± 0.78 N.kg−1at the peak and at the nadir of the day, respectively. The mean difference (95% CI) was 0.86 ± 0.47 N.kg−1 (0.62; 1.10) for the diurnal variation and 0.30 ± 0.42 N.kg−1 (0.09; 0.52) for the day-to-day variation. The mean maximum isometric arm strength was 1.68 ± 0.33 N.kg−1 at the peak and 1.46 ± 0.19 N.kg−1 at the nadir of the day, respectively. The mean difference (95% CI) was 0.21 ± 0.16 N.kg−1 (0.14; 0.29) for the diurnal variation and 0.06 ± 0.05 N.kg−1 (0.03; 0.08) for the day-to-day variation. The linear mixed-effects model showed little evidence for differences in isometric leg strength between the different times of the day (all p-values >0.983). The present study demonstrated that diurnal variations in leg and arm strength are nearly three times higher than the day-to-day variations, but there was only little evidence for a time-of-day effect on a group level. The diurnal variations observed herein without time-of-day effects are suggestive that individuals achieve their peak performance at different times of the day. Therefore, performance tests should be carried out at the same time of the day to ensure comparability. Furthermore, depending on the difference between the time of competition and the time of peak performance, as well as the individual magnitude in diurnal variation, some athletes can have a clear disadvantage.Abbreviation: 95% CI, 95% confidence interval; SD, standard deviation; ICC, intraclass correlation coefficient.
Comparative proteomics of stenotopic caddisfly Crunoecia irrorata identifies acclimation strategies to warming

Species' ecological preferences are often deduced from habitat characteristics thought to represent more or less optimal conditions for physiological functioning. Evolution has led to stenotopic and eurytopic species, the former having decreased niche breadths and lower tolerances to environmental variability. Species inhabiting freshwater springs are often described as being stenotopic specialists, adapted to the stable thermal conditions found in these habitats. Whether due to past local adaptation these species have evolved or have lost intra‐generational adaptive mechanisms to cope with increasing thermal variability has, to our knowledge, never been investigated. By studying how the proteome of a stenotopic species changes as a result of increasing temperatures, we investigate if the absence or attenuation of molecular mechanisms is indicative of local adaptation to freshwater springs. An understanding of compensatory mechanisms is especially relevant as spring specialists will experience thermal conditions beyond their physiological limits due to climate change. In this study, the stenotopic species Crunoecia irrorata (Trichoptera: Lepidostomatidae, Curtis 1834) was acclimated to 10, 15 and 20°C for 168 hr. We constructed a homology‐based database and via liquid chromatography‐tandem mass spectrometry (LC‐MS/MS)‐based shotgun proteomics identified 1,358 proteins. Differentially abundant proteins and protein norms of reaction revealed candidate proteins and molecular mechanisms facilitating compensatory responses such as trehalose metabolism, tracheal system alteration and heat‐shock protein regulation. A species‐specific understanding of compensatory physiologies challenges the characterization of species as having narrow tolerances to environmental variability if that characterization is based on occurrences and habitat characteristics alone.
New 2‐Oxoimidazolidine Derivatives: Design, Synthesis and Evaluation of Anti‐BK Virus Activities in Vitro

A series of novel 2‐oxoimidazolidine derivatives were synthesized and their antiviral activities against BK human polyomavirus type 1 (BKPyV) were evaluated in vitro. Bioassays showed that the synthesized compounds 1‐{[(4E)‐5‐(dichloromethylidene)‐2‐oxoimidazolidin‐4‐ylidene]sulfamoyl}piperidine‐4‐carboxylic acid (5) and N‐Cyclobutyl‐N′‐[(4E)‐5‐(dichloromethylidene)‐2‐oxoimidazolidin‐4‐ylidene]sulfuric diamide (4) exhibited moderate activities against BKPyV (EC50=5.4 and 5.5 μm, respectively) that are comparable to the standard drug Cidofovir. Compound 5 exhibited the same cytotoxicity in HFF cells and selectivity index (SI50) as Cidofovir. The selectivity index of compound 4 is three times less than that of Cidofovir due to the higher toxicity of this compound. Hence, these compounds may be taken as lead compound for further development of novel ant‐BKPyV agents.
General genetic and acquired risk factors, and prevalence of peri-implant diseases - Consensus report of working group 1.

For decades, oral implants have been used successfully for the replacement of missing teeth. Nevertheless, peri-implant diseases have become an increasingly important issue in daily practice. In this working group, the prevalence of peri-implant mucositis and peri-implantitis, as well as different general risk factors and their impact on the onset and progression of peri-implant diseases, were discussed based on reviews reflecting the current state of evidence. The influence of smoking on the peri-implant bone-healing process and its association with peri-implantitis has been explored in the current literature, demonstrating that smoking is an important risk indicator for the development of peri-implantitis and implant loss. Compared with non-smokers, smokers have a higher potential for pathological peri-implant bone loss, which is also influenced by poor oral hygiene. Despite the fact that a growing number of genetic polymorphisms have been identified and related to periodontal diseases, there are still no genetic patterns that could act as adjuncts to clinical diagnostics in order to identify patients at higher risk of peri-implant diseases. Long-term medications, such as bisphosphonate therapy (> 3 years), may have an impact on implant loss. A higher incidence of implant failure was reported in patients using selective serotonin reuptake inhibitors in anti-depression therapy. Alcoholism (defined as more than 5 units a day) has been associated with implant loss in retrospective and case-control studies, as well as in animal studies.
Reticulocyte Hemoglobin Equivalent (Ret-He) Combined with Red Blood Cell Distribution Width Has a Differentially Diagnostic Value for Thalassemias

Abstract As a type of congenital microcytic hypochromic anemia, thalassemia trait is often confused with other conditions, such as congenital sideroblastic anemia (CSA) and iron deficiency anemia, before a specific work-up is performed. However, these tests, including hemoglobin (Hb) electrophoresis, gene mutations and Prussian blue staining after bone marrow aspirate, are relatively expensive, time-consuming and invasive. To find labor-saving parameters to facilitate differential diagnosis, we retrospectively analyzed the routine blood indexes of 59 thalassemia trait cases [22 α-thalassemia (α-thal), 36 β-thalassemia (β-thal) and one α/β-thal], 21 CSA patients, and 238 iron deficiency anemia controls. Significantly higher reticulocyte Hb equivalent (Ret-He) and lower red blood cell (RBC) distribution width (RDW) were prominent in thalassemia trait. Furthermore, RDW-standard deviation (SD) was independent of the severity of anemia in thalassemia trait, similar to Ret-He in CSA. In the context of the same grades of anemia, Ret-He combined with RDW was powerful in differentiation of thalassemia from CSA and iron deficiency anemia. By receiver operation curve (ROC) analysis, Ret-He had a specificity of 67.06% and a sensitivity of 76.92% with a cutoff value of 20.9 pg for thalassemia trait in mild anemia and a specificity of 84.09% and a sensitivity of 68.42% with a cutoff value of 19.1 pg for thalassemia trait in moderate anemia. Regarding CSA, Ret-He had 92.94% specificity and 60.00% sensitivity in mild anemia, with a cutoff value of 18.1 pg. Overall, Ret-He and RDW are two convenient indexes able to differentiate thalassemia from the other two microcytic anemias, CSA and iron deficiency anemia.
Changing trends in surgical management of renal tumours from 2000 to 2016: a nationwide study of Medicare claims data

Guidelines recommend nephron sparing surgery where possible for patients with T1 renal tumours. The trends of nephron sparing surgery outside the USA are limited, particularly since the introduction of robotic‐assisted partial nephrectomy (RAPN). The aim of this study was to describe contemporary surgical management patterns of renal tumours in Australia according to Medicare claims data.
Neural Compatibility Modeling With Probabilistic Knowledge Distillation

In modern society, clothing matching plays a pivotal role in people’s daily life, as suitable outfits can beautify their appearance directly. Nevertheless, how to make a suitable outfit has become a daily headache for many people, especially those who do not have much sense of aesthetics. In the light of this, many research efforts have been dedicated to the task of complementary clothing matching and have achieved great success relying on the advanced data-driven neural networks. However, most existing methods overlook the rich valuable knowledge accumulated by our human beings in the fashion domain, especially the rules regarding clothing matching, like “coats go with dresses” and “silk tops cannot go with chiffon bottoms”. Towards this end, in this work, we propose a knowledge-guided neural compatibility modeling scheme, which is able to incorporate the rich fashion domain knowledge to enhance the performance of the compatibility modeling in the context of clothing matching. To better integrate the huge and implicit fashion domain knowledge into the data-driven neural networks, we present a probabilistic knowledge distillation (PKD) method, which is able to encode vast knowledge rules in a probabilistic manner. Extensive experiments on two real-world datasets have verified the guidance of rules from different sources and demonstrated the effectiveness and portability of our model. As a byproduct, we released the codes and involved parameters to benefit the research community.
Microchip System for Patterning Cells on Different Substrates via Negative Dielectrophoresis

Seeding cells on a planar substrate is the first step to construct artificial tissues in vitro. Cells should be organized into a pattern similar to native tissues and cultured on a favorable substrate to facilitate desirable tissue ingrowth. In this study, a microchip system is designed and fabricated to form cells into a specific pattern on different substrates. The system consists of a microchip with a dot-electrode array for cell trapping and patterning and two motorized platforms for providing relative motions between the microchip and the substrate. AC voltage is supplied to the selected electrodes by using a programmable micro control unit to control relays connected to the dot-electrodes. Nonuniform electric fields for cell manipulation are formed via negative dielectrophoresis (n-DEP). Experiments were conducted to create different patterns by using yeast cells. The effects of different experimental parameters and material properties on the patterning efficiency were evaluated and analyzed. Mechanisms to remove abundant cells surrounding the constructed patterns were also examined. Results show that the microchip system could successfully create cell patterns on different substrates. The use of calcium chloride (CaCl$_2$) enhanced the cell adhesiveness on the substrate. The proposed n-DEP patterning technique offers a new method for constructing artificial tissues with high flexibility on cell patterning and selecting substrate to suit application needs.
A Prior High-Intensity Exercise Bout Attenuates the Vascular Dysfunction Resulting From a Prolonged Sedentary Bout.

BACKGROUND This study sought to determine the impact of an acute prior bout of high-intensity interval aerobic exercise on attenuating the vascular dysfunction associated with a prolonged sedentary bout. METHODS Ten young (24 ± 1 y) healthy males completed two 3-hour sessions of prolonged sitting with (SIT-EX) and without (SIT) a high-intensity interval aerobic exercise session performed immediately prior. Prior to and 3 hours into the sitting bout, leg vascular function was assessed with the passive leg movement technique, and blood samples were obtained from the lower limb to evaluate changes in oxidative stress (malondialdehyde and superoxide dismutase) and inflammation (interleukin-6). RESULTS No presitting differences in leg vascular function (assessed via passive leg movement technique-induced hyperemia) were revealed between conditions. After 3 hours of prolonged sitting, leg vascular function was significantly reduced in the SIT condition, but unchanged in the SIT-EX. Lower limb blood samples revealed no alterations in oxidative stress, antioxidant capacity, or inflammation in either condition. CONCLUSIONS This study revealed that lower limb vascular dysfunction was significantly attenuated by an acute presitting bout of high-intensity interval aerobic exercise. Further analysis of lower limb blood samples revealed no changes in circulating oxidative stress or inflammation in either condition.
Development and Validation of a Model to Predict Acute Kidney Injury in Hospitalized Patients With Cirrhosis

OBJECTIVES: Acute kidney injury (AKI) is a common complication in hospitalized patients with cirrhosis which contributes to morbidity and mortality. Improved prediction of AKI in this population is needed for prevention and early intervention. We developed a model to identify hospitalized patients at risk for AKI. METHODS: Admission data from a prospective cohort of hospitalized patients with cirrhosis without AKI on admission (n = 397) was used for derivation. AKI development in the first week of admission was captured. Independent predictors of AKI on multivariate logistic regression were used to develop the prediction model. External validation was performed on a separate multicenter cohort (n = 308). RESULTS: In the derivation cohort, the mean age was 57 years, the Model for End-Stage Liver Disease score was 17, and 59 patients (15%) developed AKI after a median of 4 days. Admission creatinine (OR: 2.38 per 1 mg/dL increase [95% CI: 1.47–3.85]), international normalized ratio (OR: 1.92 per 1 unit increase [95% CI: 1.92–3.10]), and white blood cell count (OR: 1.09 per 1 × 109/L increase [95% CI: 1.04–1.15]) were independently associated with AKI. These variables were used to develop a prediction model (area underneath the receiver operator curve: 0.77 [95% CI: 0.70–0.83]). In the validation cohort (mean age of 53 years, Model for End-Stage Liver Disease score of 16, and AKI development of 13%), the area underneath the receiver operator curve for the model was 0.70 (95% CI: 0.61–0.78). DISCUSSION: A model consisting of admission creatinine, international normalized ratio, and white blood cell count can identify patients with cirrhosis at risk for in-hospital AKI development. On further validation, our model can be used to apply novel interventions to reduce the incidence of AKI among patients with cirrhosis who are hospitalized.
The temporal and long‐term impact of donor body mass index on recipient outcomes after kidney transplantation – a retrospective study

The impact of increasing body mass index (BMI) on development and progression of chronic kidney disease is established. Even implantation kidney biopsies from obese living donors demonstrate subtle histologic changes despite normal function. We hypothesized that kidneys from obese living (LD) and deceased donors (DD) would have inferior long‐term allograft outcomes. In a study utilizing US transplant registry, we studied adult kidney transplant recipients from 2000 to 2014. Donors were categorized as BMI <20 (underweight), 20–25 (normal), 25–30 (overweight), 30–35 (mildly obese), and >35 kg/m2 (very obese). Our outcome of interest was death censored graft failure (DCGF). Cox proportional hazards model were fitted separately for recipients of DD and LD kidneys, and adjusted for donor, recipient, and transplant characteristics, including donor and recipient size mismatch ratio. Among 118 734 DD and 84 377 LD transplants recipients, we observed a significant and graded increase in DCGF risk among the overweight (LD:HR = 1.06, DD:HR = 1.04), mildly obese (LD:HR = 1.16, DD:HR = 1.10), and very obese (LD:HR = 1.22, DD:HR = 1.22) compared to normal BMI (P < 0.05). The graded effect of donor BMI on outcomes begins early and persists throughout the post‐transplant period. Donor obesity status is an independent risk factor for inferior long‐term renal allograft outcome despite adjusting for donor and recipient size mismatch and other donor, recipient, and transplant factors.
Alcohol Problems, Jealousy, and Cyber Dating Abuse Perpetration Among Men and Women: Toward a Conceptual Model

Investigations of alcohol use in relation to cyber dating abuse (CDA) remain underdeveloped relative to alcohol-related face-to-face dating abuse research. A critical step toward advancing this area of research would include examining the applicability of alcohol-related partner abuse models to CDA perpetration. Existing models of alcohol-related partner abuse suggested that alcohol and partner abuse are more likely to co-occur in the presence of aggressogenic distal traits. We propose that this model may extend to CDA perpetration. Toward this end, the present study collected cross-sectional data from college students (N = 258; 56.2% male) to investigate whether trait romantic jealousy moderated the association between alcohol problems and CDA perpetration, controlling for face-to-face dating abuse perpetration. We hypothesized that alcohol problems would positively relate to CDA perpetration among college students with high, but not low, romantic jealousy. We explored whether the interactive effect varied by sex. Results revealed a significant three-way interaction; the moderating role of romantic jealousy in the relation between alcohol problems and CDA perpetration varied by sex. Alcohol problems positively related to CDA perpetration for women with high, but not low, romantic jealousy. Alcohol problems did not relate to CDA perpetration regardless of men’s level of romantic jealousy. These preliminary results suggested that alcohol-related partner abuse models may be useful for conceptualizing CDA perpetration and identifying CDA intervention components.
Ring Difference Filter for Fast and Noise Robust Depth From Focus

Depth from focus (DfF) is a method of estimating the depth of a scene by using information acquired through changes in the focus of a camera. Within the DfF framework of, the focus measure (FM) forms the foundation which determines the accuracy of the output. With the results from the FM, the role of a DfF pipeline is to determine and recalculate unreliable measurements while enhancing those that are reliable. In this paper, we propose a new FM, which we call the “ring difference filter” (RDF), that can more accurately and robustly measure focus. FMs can usually be categorized as confident local methods or noise robust non-local methods. The RDF’s unique ring-and-disk structure allows it to have the advantages of both local and non-local FMs. We then describe an efficient pipeline that utilizes the RDF’s properties. Part of this pipeline is our proposed RDF-based cost aggregation method, which is able to robustly refine the initial results in the presence of image noise. Our method is able to reproduce results that are on par with or even better than those of state-of-the-art methods, while spending less time in computation.