Miracle-dz/UniFAR-FTUs huggingface dataset:
THE EFFECT OF SUBINHIBITORY ANTIBIOTICS CONCENTRATION (SUB-MIC) ON BIOFILM FORMATION BY METHICILLIN RESISTANCE STAPHYLOCOCCI (USING OF ANTIBIOTICS ALONE AND IN COMBINATION) -

The ability to produce slime layer by Methicillin-resistant S. aureus (MRSA) and Methicillin-Resistant Coagulase-Negative Staphylococci (MR-CONS) isolates was investigated and the results showed that all isolates of both MRSA and MR-CONS were produced slime layer when tested by tube method but the amounts of adherent materials were different among the isolates. However, the results by Congo red agar method showed that 60% of MRSA isolates produced strong slime layer and 40% of MRSA obtained negative result, and in the case of MR-CONS all tested isolates (100%) had the ability to produce strong slime layer by this method. Similarly the ability of MRSA and Methicillin-Resistant Staphylococcus epidermidis (MRSE) isolates to produce biofilm by tissue culture plate (TCP) was investigated and the results indicated that both MRSA and MRSE showed highly and strong biofilm formation, and the OD value of biofilm formation ranged between 0.520 - 0.738, and the OD value obtained from MRSE isolates were higher compared to the MRSA isolates. Additionally the OD value of biofilm formation significantly increased in addition of 1% glucose to the media. The results showed that there are a possible correlation between Methicillin susceptibility and the biofilm formation in S. aureus isolates. The effect of Subinhibitory Antibiotics Concentration (sub-MIC) of two antibiotics (Vancomycin and Clindamycin) on the biofilm formation by MRSA and MRSE isolates were investigated and the results showed that both antibiotics at two subinhibitory concentration (one-half and two-half the MIC) were significantly inhibitory to biofilm formation by both isolates when using of each antibiotic alone, but Vancomycin was most effective than Clindamycin. Additionally, the results showed that the combination of these two antibiotics at different sub-MIC were significantly reduced or inhibited the biofilm formation by both isolates. In comparison between the using of each antibiotics alone or in combination the results showed that in the case of MRSE isolate there was no significant difference between the two treatment methods, while in the MRSA isolate there was a significant difference between the two methods. The using of each antibiotic alone (Vancomycin or Clindamycin) was most effective than the using of combination of these two antibiotics in the inhibition of biofilm formation.
Resistance of triticale to wheat leaf rust (Puccinia triticina)

Reactions of winter triticale cultivars mostly from central Europe to recent and old leaf rust isolates were tested in the greenhouse. In one trial 20 cultivars were tested with 8 leaf rust isolates, collected recently from official wheat and triticale trials in the Czech Republic and Slovakia. In another trial 15 cultivars were tested with 6 old leaf rust isolates, used to identify leaf rust resistance genes in wheat. The cultivars Cando, Hortenso and Tricolor, registered in the Czech Republic, were resistant to the majority of the recent isolates. The Hungarian cultivar Tatra was resistant to all the recent isolates. The old leaf rust isolates were virulent only to a few of the triticale cultivars. Randomly selected isolates from wheat and triticale were tested on triticale cultivars and on Thatcher near isogenic lines with different lr genes. On average, leaf rust isolates from triticale were virulent to a higher number of triticale cultivars than isolates collected from wheat and vice versa.
Consumer Behaviour, Marketing Strategy, Customer Satisfaction, and Business Performance

ABSTRACT The purpose of this research is to examines the influence of consumer behariour on marketing strategy, customer satisfaction and business performance, the influence of marketing strategy on customer satisfaction and business performance, and the influence of customer satisfaction on business performance The population used in this study were guests who stayed in a Villa unit sand Hotels under the management of Alpha Hotel Management, Bali – Indonesia, with a total of 171 respondents. Data were collected by using questionnaire, where the sampling method in this study using combination of purposive and accidental sampling.Technical analysis used is a quantitative analysis with SEM (Structural Equation Model) analysis method using AMOS. The results showed that consumer behavior has a positive and significant impacts on marketing strategy and customer satisfaction, positively insignificant impact on business performance. Marketing strategy has a positive significant impacts on customer satisfaction, but negative insignificants impact on business performance. While customer satisfaction has a positive and significant impact on business performance. Research also figure out that marketing strategy and customer satisfaction strengthen the impact of consumer behavior on business performance. Reseracher conclude that in order to gain better business performance, the marketer of a company has to combining all variables (Consumer Behaviour, Marketing Strategy, and Customer Satisfaction) rather than just focusing their efforts only on Marketing Strategy, where ensuring the highest level of Customer Satisfaction should become the most essential efforts to gain the highest Business Performance. Researcher suggest that future study be carried out using the same methodology but encompassing bigger areas of population and sampling, and uses more variables and indicators .
Meiotic Dna Re-Replication And The Recombination Checkpoint

MEIOTIC DNA RE-REPLICATION AND THE RECOMBINATION CHECKPOINT by NICOLE A. NAJOR December 2010 Advisor: Dr. George S. Brush Major: Pharmacology Degree: Doctor of Philosophy Progression through meiosis occurs through a strict sequence of events, so that one round of DNA replication precedes programmed recombination and two nuclear divisions. Cyclin dependent kinase 1 (Cdk1) is required for meiosis, and any disruption in its activity leads to meiotic defects. The Cdk1 inhibitor, Sic1, regulates the G1-S transition in the mitotic cell cycle and the analogous transition in meiosis. We have employed a form of Sic1, Sic1∆P, that is mutated at multiple phosphorylation sites and resistant to degradation. Meiosis specific expression of Sic1∆P disrupts Cdk1 activity and leads to significant accumulation of over replicated DNA. These data suggested that Cdk1 is required to prevent inappropriate re-initiation of DNA synthesis during meiosis, as it is during mitosis. In addition, deletion of the gene DMC1, which encodes a recombinase required for meiotic recombination, prevented DNA re-replication.
Anti-inflammatory activity of ixora coccinea methanolic leaf extract

The anti-inflammatory activity of methanolic leaf extract (MLE) of Ixora coccinea Linn. (Rubiaceae) was investigated in this study. MLE showed dose-dependent anti-inflammatory activity in carrageenan-induced rat paw edema model (r = 0.7; P 50 value = 8.0 μg/ml), membrane stabilizing activity (r = 0.81; P 50 value = 6.4 ng/ml) and lipid peroxidation activity (36.7% at 250 μg/ml; P<0.01). Thirty-day oral treatment of rats with 1500 mg/kg did not show any adverse signs of toxicity or behavioral changes. These results suggest that anti-inflammatory activity of I. coccinea is mediated via inhibition NO production, phagocytic cell infiltration, anti-histamine effect, scavenging of free radicals, membrane stabilizing activity and lipid peroxidation.
Biochemical studies on trypanosomal prolyl oligopeptidase family pathogenic factors.

iv ACKNOWLEDGEMENTS vi LIST OF FIGURES x LIST OF TABLES xiii ABBREVIATIONS xiv CHAPTER 1 Literature Review 1 1.1. Trypanosomosis 1 1.1.1 Trypanosomes and their classification 1 1.1.2 Trypanosome life cycle and mechanism of infection 3 1.1.3 Antigenic variation 5 1.1.4 Trypanosome distribution and vector control 5 1.1. 5 Trypanotolerance and chemotherapy 7 1.2. Proteolytic peptidases in trypanosomes 8 1.2. 1 Serine peptidases 9 1.2. 2 Prolyl Oligopeptidase 12 1.2. 3 POP active site 15 1.2. 4 POP catalytic mechanism 16 1.2. 5 Oligopeptidase B (OPB) 16 1.2. 6 OPB active site and catalytic mechanism 17 1.3. Role of POP and OPB in trypanosomosis pathogenesis 19 1.4. Objectives of the present study 20 CHAPTER 2 Cloning, recombinant expression and purification of Trypanosoma brucei brucei and T. congolense Prolyl Oligopeptidase 22 2.
Effects of sodium selenite supplementation on lead nitrate-induced oxidative stress in lung tissues of diabetic and non-diabetic rats

In this study, diabetic and non-diabetic male rats were given to sodium selenite, lead nitrate and sodium selenite plus lead nitrate through gavage. At the end of the 4 th week, lipid peroxidation and antioxidant enzyme activities was investigated compared to control group. No significant differences were observed between control and sodium selenite treated groups. By the end of the fourth week, lead nitrate led to increase the levels of MDA, and decrease in antioxidant activities compared with the control group in diabetic and non-diabetic rats. As a result, sodium selenite significantly reduces lead nitrate induced testicular toxicity both groups.
Analyse moléculaire de la diversité génétique des dromadaires (Camelus dromedarius) en Tunisie

Cette etude s’est interessee a l’investigation de l’analyse de la diversite et des relations phylogeniques entre les populations de dromadaires dans trois regions arides du Sud tunisien (Kebili, Medenine et Tataouine). Huit marqueurs microsatellites ont ete utilises pour genotyper 90 individus. Au total, 34 alleles ont ete detectes dans les trois populations. Le nombre d’alleles par locus varie de 2 a 7, avec une moyenne de 4,25 alleles par locus. Pour chaque population, la moyenne d’alleles par locus est de 3,33 ; 3,71 et 3,87 pour Kebeli, Medenine et Tataouine, respectivement. Le taux de l’heterozygotie observee est de 0,43 ; 0,50 ; 0,57 et 0,52 pour Kebeli, Medenine, Tataouine et la population totale, respectivement. Ces valeurs sont inferieures a celles de l’heterozygotie attendue : 0,50 ; 0,57 ; 0,62 et 0,61, respectivement. Les coefficients de consanguinite sont de 15,3 % pour Kebeli, 11,4 % pour Medenine et 8,3 % pour Tataouine. Les F-sat moyens sont FIT = 0,15, FIS = 0,071 et FST = 0,083, ces valeurs sont significativement differentes de zero (p < 0,05) et suggerent une differentiation moderee. Un taux de consanguinite de 15 % dans la population totale a ete trouve. Les distances genetiques estimees varient de 0 a 0,9 entre les individus de la population totale. Les distances genetiques entre les paires des populations indiquent 0,104 entre Medenine et Tataouine, 0,280 entre Kebili et Medenine et 0,290 entre Kebeli et Tataouine. Cette matrice de distance permet de distinguer deux entites genetiques : Nefzawa (Kebili) qui inclut les ecotypes Merzougui, G’oudi et Mehari et le groupe de Aaradh (Medenine et Tataouine) qui inclut Magherbi et Khaouar. Les resultats de ce travail ne confirment pas la classification actuelle etablie par les eleveurs qui divisent la population en cinq ecotypes selon des criteres sociogeographiques. Ces resultats preliminaires montrent que les microsatellites sont un outil prometteur pour la caracterisation des races animales. Ils indiquent que la population etudiee a une diversite genetique satisfaisante qui peut etre utilisee comme base pour amenager des programmes de conservation et de developpement durable de l’espece.
Comparing and developing statistical methods for fine-mapping genes in complex diseases

This project is an investigation of current and novel methods of analysing genotype data from fine-mapping association studies, where a single, unknown causal variant is present in a genomic region known to be associated with disease risk. The analysis methods are all univariate and can be used to filter the genetic variants in an association region, reducing them to a set of candidate causal variants. Initially, a number of methods including p-value, likelihood, genetic map distance and linkage disequilibrium based analyses are compared using simulated data. A method that we call likelihood percentile is found to generally be the most effective, in various scenarios that may occur in fine-mapping studies. I also investigate the effects of varying such things as the causal variant, genomic region and genetic effect on the efficacy of likelihood percentile filtering. To explain the difference in filtering results using two different likelihood based methods, I consider the likelihood surface as a function of the numbers of case and control subjects with the risk genotype. In addition, I examine the effect of imputing missing genotype dosage, as is common practice in fine-mapping studies, on the efficacy of variant filtering. The use of Bayes factors to filter genetic variants is investigated, assuming that a prior on the natural logarithm of the odds ratio of the form N ∼ (0,W ) is considered appropriate. It is shown that filtering efficacy is sensitive to W , so several methods of informing the choice of W are compared. These include defining W as a function of minor allele frequency, an empirical Bayes method and several novel forms for the Bayes factor which put a prior on W , thus taking into account prior uncertainty about W . Several appropriate methods are applied to a large dataset of a finemapped region from the Collaborative Oncological Gene-environment Study.
Genetic Diversity Analysis of Cultured Populations of Jade Perch (Scortum bacoo) in China Using AFLP Markers

Genetic diversity of three cultured populations of jade perch (Scortum bacoo) are studied using amplified fragment length polymorphism (AFLP) markers, which is Guangzhou(GZ) population, Foshan (FS) population and Qingdao(QD) population. Nine primer combinations were used and 385 fragments were detected. Among the 385 fragments, 80 bands (20.78%) were polymorphic. And it can be speculated that the genetic diversity of the three cultured populations of jade perch was very poor according to the gene genetic diversity among populations (Ht), the gene genetic diversity within populations (Hs) and Shannon-Weiner index (I). From gene differentiation (Gst), genetic distance (D), genetic similarity (S), and UPGMA analysis, it is found that the relationship among the three populations is very closed, and the difference in genetic diversity among the three populations is not significant. Further clustering relationships of the jade perch cultured populations also are correlated to historical-breeding observations and genetic relationships. The GZ population clustered together with the QD population first, then with FS population.
Antibiotic Resistance in Staphylococcus Species of Animal Origin

Staphylococcus is a genus of worldwide distributed bacteria correlated to several infectious of different sites in human and animals. Its importance is not only because of its distribution and pathogenicity but especially due to its ability to overcome antimicrobial effects. The goal of this chapter is to report data obtained from a decade of research in animal science field concerning to staphylococci antimicrobial resistance.
Presence of an Albumin-Like Protein on the Plasma Membrane of Adipocytes Isolated from Korean Cow

The main function of adipocytes in various species is to store nutrient energy in the form of triglycerides, and this function may he closely related with hormonal signaling through the plasma membrane of adipocytes. Using SDS-PAGE, two-dimensional gel electrophoresis, and a membrane biotinylation technique, we have identified a 55KDa protein (55K protein) from the plasma membrane fraction of adipocytes, with an isoelectric point (pI) of 8.1-8.3. However, this 55K protein was not observed with a two-dimensional gel electrophoresis carried out on plasma membrane fractions prepared from the liver, heart, and kidney tissues. An analysis of the 12 amino acids sequence at the N-terminal of the 55K protein showed that it has a similar sequence to that of bovine serum albumin.
Atividade citotóxica de extratos brutos de Lippia alba (Mill.) N.E. Brown (Verbenaceae)

RESUMO. Extratos brutos da raiz, caule e folhas de Lippia alba (Mill.) N.E. Brown (Verbenaceae), foram testados frente as celulas NCI-H292 (obtidas de carcinoma mucoepidermoide de pulmao humano) e celulas HEp-2 (derivadas de carcinoma epidermoide de laringe). O extrato etanolico da folha mostrou-se mais citotoxico frente as celulas HEp-2 com CI50 igual 8,17 µg/mL. Para as celulas NCI-H292 observou-se que o extrato cloroformico da raiz foi o mais citotoxico, com CI 50 igual 4,64 µg/mL. Os constituintes com maiores acoes antineoplasica estao presentes, principalmente, na raiz e folhas da especie. SUMMARY. “ Citotoxic Activity of Crude Extracts of Lippia alba (Mill.) N.E. Brown (Verbenaceae)” . Crude extracts of Lippia alba (Mill.) N. E. Brown (Verbenaceae) roots, stem and leaves, were tested against cells lines of the pulmonary mucoepidermoid human carcinoma (NCI-H292) and cells lines derived from an epidermoid carcinoma of the human larynx (HEp-2). Ethanolic extract of the leaves showed more citotoxicity against HEp-2 cells with CI50 = 8.17 µg/mL. In the case of NCI-H292 cells the chloroformic extract of the root was the most citotoxic, with CI50 = 4.64 µg/mL. The constituents with higher antineoplasic action are present mostly in the root and leaves of the studied species.
Effect of two Preparation Designs and Methods of Construction on the Fracture Resistance of Glass Ceramics Laminate Veneers

Purpose: To evaluate the effect of marginal finish line design and fabrication techniques on the fracture resistance of lithium disilicate laminate veneer. Materials and methods: A total of 24 veneers were designed, constructed, and divided into two main groups according to preparation design Chamfer 0.3 mm finish line (n=12) and, Chisel finish line (n=12). Each group was then divided into two subgroups according to the technique of veneer construction, veneers constructed by (CAD/CAM system) out of e max CAD blocks (n=6), and veneers constructed by (press on technology) using e max press ingots (n=6). then the veneers bonded to corresponding epoxy resin dies using resin cement. Finally fracture resistance test was done for all specimens using universal testing machine. Results: The highest fracture resistance values were recorded in the group of chisel marginal design fabricated by CAD/CAM technique. The lowest fracture resistance values were recorded in the group of 0.3mm chamfer margin design fabricated by Press technique. Regardless to margin design there was a significant difference between both processing techniques as indicated by two-way ANOVA test (p=0.0178 Press). Irrespective of material type it was found that margin design significantly influenced mean as indicated by two-way ANOVA test (p=0.0001 0.05). It was found that the Chamfer margin design recorded statistically significant higher fracture resistance mean value with CAD/CAM group (245.85±56.37N) than Press group (129.71±19.49 N) as indicated by unpaired t-test (P=0.002<0.05) Conclusions: The technique of fabrication of ceramic laminate veneer restorations has a crucial effect on its performance with the regard to fracture resistance. IPS e.max CAD could be considered as a valid restoration than IPS e.max press.
Outils d'évaluation d'une intervention d'éducation pour la santé environnementale périnatale

De nombreuses affections chez l’enfant et l’adulte sont associees a une exposition in utero a des perturbateurs endocriniens (PE). Pour reduire cette exposition, des programmes d’education pour la sante environnementale perinatale se developpent. Ils font evoluer des dimensions psychosociales que sont la perception du risque (PR) et la croyance en l’action de reduction de l’exposition (CAR), mais peu d’entre eux sont evaluesL’etude de recherche interventionnelle PREVED a pour objectif d’evaluer l’efficacite d’un programme d’education pour la sante environnementale pour des femmes enceintes, visant a diminuer leur exposition aux PE.Les objectifs de ces travaux metrologiques etaient de developper des outils (i) analytiques et (ii) epidemiologiques en vue d’evaluer l’efficacite du programme. Ainsi, (i) des methodes analytiques ultrasensibles par LC-MS/MS permettant de doser les fractions non conjuguees de PE dans les urines et le colostrum ont ete developpees et valides sur des prelevements recueillis dans le cadre de la cohorte perinatale EDDS; (ii) un questionnaire psychosocial explorant la PR, la CAR et les connaissances des femmes enceintes au moyen de scores a ete concu.Ces travaux s’inscrivent dans une approche interdisciplinaire de sante environnementale. Ils proposent des methodes analytiques fiables pour mesurer l’exposition aux PE etudies d’une part, et un questionnaire evaluant connaissances, attitudes et pratiques des femmes enceintes sur la question des PE d’autre part. Ces outils permettront de mesurer l’impact du programme d’education pour la sante environnementale perinatale.
To be competent or not: an inquiry into the molecular basis of bacterial differentiation

The Gram-positive bacterium Bacillus subtilis demonstrates a remarkable ability to differentiate into different cell types. One of these differentiation processes is competence for natural transformation: the ability to take up DNA from the environment and stably integrate it into the genome. Although all cells in a B. subtilis culture are genetically identical, only a subpopulation of cells develops competence. The process is governed by the key transcriptional regulator ComK. In this dissertation the molecular mechanisms responsible for the separation into subpopulations, the regulatory inputs for ComK and the relation between competence and other differentiation process are addressed.
The Hcs7 Mouse Liver Cancer Modifier Maps to a 3.3 Mb Region Carrying the Strong Candidate Ifi202b

Genes that affect a person's chance of developing hepatocellular carcinoma (HCC), as BRCA1 and BRCA2 affect a person's chance of developing breast or ovarian cancer, have been difficult to detect. The vast majority of liver cancers can be attributed to Hepatitis B or C virus infection, aflatoxin exposure, or alcoholic cirrhosis, alone or in combination (Montalto et al., 2002). This high background of predisposing environmental factors makes identifying less penetrant genetic contributors more difficult. Familial patterns of susceptibility to liver cancer independent of environmental factors have helped identify a few monogenic metabolic syndromes (e.g., hemochromatosis; Dragani, 2010). However, the analysis of liver tumors points to a variety of other genes that affect liver tumor development. The patterns of chromosome gain and loss in liver cancers worldwide reveal several regions that are gained or lost in up to 86% of tumors, including gains of 1q, 6p, 8q, and 20q, and losses of 1p, 4q, 6q, 8p, 13q, 16q, and 17p (Lau and Guan, 2005; Chochi et al., 2009; Zhang et al., 2010). Chromosome analyses combined with genome-wide association studies have revealed candidate HCC modifier genes for some of these regions, such as PAPSS1 on chromosome 4q and HCAP1 on chromosome 17p (Wan et al., 2004; Shih et al., 2009).