Miracle-dz/UniFAR-FTUs huggingface dataset:
High Stability in Chromosomal Traits of Chamaelirium japonicum and C. koidzuminum (Melanthiaceae) with Holocentric Chromosomes

Chromosome number, morphology, and behavior in the mitotic cycle were examined for 334 individuals from 34 local populations of Chamaelirium japonicum, including two infraspecific taxa so far chromosomally uninvestigated, and for 20 individuals from three populations of C. koidzumianum, including one variety cytologically unexplored to date. All of the individuals proved to have holocentric chromosomes, and 351 out of the 354 individuals exhibited 2n=24=4L+20S (where L and S denote ‘long’ and ‘short,’ respectively) and were regarded as diploids (2x). Of the other three individuals of C. japonicum ssp. japonicum, two were triploids (3x) with 2n=36=6L+30S, and one was a hyperploid (2x+1) with 2n=25=4L+19S+2f, of which the two small chromosomes (f) presumably arose by fission in one short chromosome. The triploid and the hyperploid showed lower pollen stainability (16.3 and 71.7%, respectively) with cotton blue than normal diploids (98.0–99.5%). Factors leading to the high stability of chromosomal traits in the two species are suggested.
Unfolded Histidine-Tagged Protein is Immobilized to Nitrilotriacetic Acid-Nickel Beads, But Not the Nickel-Coated Glass Slide

The adsorption of proteins on the surface of glass slides is essential for construction of protein chips. Previously, we prepared a nickel-coated plate by the spin-coating method for immobilization of His-tagged proteins. In order to know whether the structural factor is responsible for the immobilization of His-tagged proteins to the nickel-coated glass slide, we executed a series of experiments. First we purified a His-tagged protein after expressing the vector in E. coli BL21 (DE3). Then we obtained the unfolding curve for the His-tagged protein by using guanidine hydrochloride. Fractions unfolded were monitored by internal fluorescence spectroscopy. The for unfolding was . Then we tested if unfolded His-tagged proteins can be adsorbed to the nickel-coated plate, comparing with (nitrilotriacetic acid) beads. Whereas unfolded His-tagged proteins were adsorbed to beads, they did not bind to the nickel-coated plate. In conclusion, a structural factor is likely to be an important factor for constructing the protein chips, when His-tagged proteins will immobilize to the nickel-coated slides.
Co-creation of social services on the example of social hackathon: The case of Estonia

Drawing on three semi-structured focus group interviews with a sample of 14 social hackathon participants in southern Estonia, this article reports the findings of participants’ experiences of the co-creation process in developing new services or innovative solutions to community problems by using a method of social hackathon. In general, hackathon was perceived as a creative and ‘out-of-the-box’ type of approach, a non-traditional way of thinking and an openness to constructing solutions in a new and creative way. Furthermore, the hackathon was recognised as a fitting tool for the social worker to find solutions in the community with service users.
The effects of kinesiotaping applied to delayed-onset muscle soreness of the quadriceps femoris

The aim of this work was to estimate the effectiveness of kinesiotaping (KT) in the reduction of delayed-onset muscle soreness (DOMS) of the quadriceps femoris. 41 young women were examined. In the study, two groups applied kinesiotaping – the first with a relaxing application (Group 1); and the second with a lymphatic one (Group 2). Women from the control group used no treatment to help in the exertion restitution. All of the women performed a vertical jump test and training of their lower limbs (5 series of squat jumps). The research tools were the VAS scale (for the estimation of pain) and the Borg Scale (for a subjective estimation of the intensity of effort). The measurements (the vertical jump test and the intensification of the pain) were repeated 24, 48, 72 and 96 hours after the training where the exertion occurred. In all of the groups, the greatest regress of the vertical jump test was observed between the first and the second measurement – and this difference was statistically essential (p<0.01). The least fall in power was noticed in the women who had received the lymphatic KT application; while the greatest fall in results of vertical jump test was observed in the persons with the KT relaxing application. The greatest level of pain in Groups 1 and 2 was noted during the second measurement, which was 24 hours after the training; whereas in the control group, the greatest pain was observed in the third measurement. It can be concluded that the kinesiotaping has a bearing on the acceleration of the regeneration and increased the efficiency of the examined muscles.
Molecular analysis of importin-α-mediated nucleocytoplasmic signaling in plant innate immunity

IMPORTIN-αs are a family of nuclear transport receptors that mediate the translocation of nuclear localization signal (NLS)-containing cargo proteins from the cytoplasm into the nucleus through nuclear pores. The IMPORTIN-α3, MOS6 (MODIFIER OF SNC1, 6), is one of nine putative IMPORTIN-αs encoded by the Arabidopsis genome. MOS6 was identified as an essential component of auto-immune responses and related growth inhibition caused by the constitutively active TIR-NB-LRR Resistance protein variant, snc1 (suppressor of npr1-1, constitutive 1). In addition, MOS6 is required for basal resistance. This suggests that MOS6 is required for specific or preferential nuclear import of unknown cargo proteins involved in defense signaling. In this study, the contribution of the nine IMPORTIN-α family members to basal resistance and snc1-mediated auto-immunity was investigated and potential functional redundancies within the nuclear import receptor family in plant defense signaling were addressed. For reverse-genetic analyses, a collection of importin-α single, double and triple mutant combinations was generated and a prominent role of MOS6 in plant immunity and snc1 auto-immunity could be demonstrated. To identify defense-related cargo substrates and interaction partners of MOS6, in planta affinity purification of functional epitope-tagged MOS6 coupled with mass spectrometry was performed in addition to analyses of an established Arabidopsis interactome database. From these approaches, thirteen candidate MOS6 interactors were selected for further characterization. Transient expression in Nicotiana benthamiana and subsequent co-immunoprecipitation was used to validate these interactions. Several interactors were shown to selectively bind MOS6 as they did not interact with its closest homolog IMPORTIN-α6, reinforcing the idea of MOS6 substrate specificity. T-DNA insertion mutants of MOS6-interactor candidates were isolated and subjected to functional analyses. Interestingly, a mutation in the gene encoding the TIR-NBS protein TN13 leads to impaired resistance against Pseudomonas syringae pv. tomato DC3000 with an incomplete effector repertoire (ΔAvrPto/AvrPtoB). This finding demonstrates a so far unknown involvement of the novel MOS6-interacting protein TN13 in plant innate immunity. Confocal laser scanning microscopy revealed that TN13 localizes to the endoplasmic reticulum when transiently expressed in N. benthamiana. This may suggest release of TN13 from the ER-membrane upon pathogen attack and subsequent MOS6-mediated nuclear translocation for maintenance of basal resistance.
Managing relational conflict by closing the intention and behavioural gap through the use of a 3 dimensional visualisation and simulation model

Abstract Disappointment in interpersonal relationships may arise from a gap between the intentions and actions or perceptions that each person has of their own intentions and actions and those of others (Intention and Behaviour Gap). This gap can originate from: (a) a diminished or lack of insight or awareness of own emotions and behaviours, and it’s effect on others, (b) lack of awareness of the intention or the desired outcomes and (c) the gap existing between the two people as well as the (d) inability to change behaviour. This gap could be related to behaviours people have that have become known and culturally accepted as rigid and inflexible. Consequently, the person focuses on their intention with little to no self-knowledge of the displayed behaviour, thus judging their own actions on their good personal intention while judging others based on their perceptions rather than the others’ intentions. This inclination amounts to self-righteousness and can increase the likelihood of frustration and anger towards the perceived bias and lack of fairness towards others. In turn, this might cause interpersonal discord, resentment, misunderstanding, and conflict. To address this behavioural gap, this study proposes the application of a 3-Dimensional, Visualisation and Simulation Model (3DVSM) to enhance insight into own and others behaviours and actions, followed by connecting one’s actions to the desired intention. The ultimate goal is to instil a desire for modifying one’s behaviour, thus reducing interpersonal conflict by shortening the gap between intention and action.
Comparison between active and passive immunization with flagellin-based subunit vaccine from Pseudomonas aeruginosa in the burned-mouse model.

Objective: Pseudomonas aeruginosa is an opportunistic pathogen that infects hospitalized, burned and immunosuppressed patients. The main aim of the present study was to develop a vaccination strategy based on recombinant flagellin type A (r-fla-A) that would enhance the protective response against P. aeruginosa in the burn wound sepsis model. Materials and Methods: After the preparation of type A r-flagellin, a specific polyclonal IgG was produced in rabbit. After immediate post-burn and post-challenge, mortality rate was screened in the mice treated with anti r-fla-A IgG, and inbred mice were also immunized with r-fla-A in separate groups. After final booster, vaccinated mice were burned and challenged with P. aeruginosa. The functional activity of anti r-flagellin antisera was determined by opsonophagocytic killing test. To evaluate the humoral immune response, sera were analyzed by ELISA for its total antibody. Results: In vivo administration of r-fla-A afforded a remarkable improvement in the survival of mice challenged with homologous strain (PAK) in the burn wound infection (83.3% vs. 0% in control; P < 0.005). The antibodies generated against the r-fla-A achieved 25% survival in immunized mice that were infected with heterologous strain PAO1. The anti r-fla-A IgG afforded a significant improvement in survival of mice infected by homologous strain PAK from 16.6% to 75%; In contrast, this antiserum achieved 33.3% survival following challenge with heterologous strain PAO1 (compared to control IgG). Anti r-flagellin antibody promoted phagocytosis of the homologous strain and decreased the killing of heterologous strain (53.1% and 17.4% respectively vs. 3.7% in the control group; P < 0.001). Flagellin also induced a high level humoral immune response in the immunized burned and challenged mice. Conclusion: We concluded that immunization with r-fla-A and anti r-fla-A would protect burned mice against lethal P. aeruginosa challenge.
Tackling Complex Social Challenges within Neoliberal Constraints: The Context Shaping ‘Intellectual Quality of Life’ (iQoL) in a Canadian University Context

The contemporary academic environment in Canada has undergone reorganization based on neoliberal principles, and has increased attention focused on the importance of supporting interdisciplinary initiatives to address complex problems affecting global society. The purpose of our study was to examine the experience of people participating in a specific university-funded interdisciplinary research initiative. As there is a strong emphasis within this program on reporting on the outcomes of the funding that supports interdisciplinary collaboration, our aim was to explore how participation may shape one’s intellectual quality of life (iQoL) and how one’s iQoL could be conceptualized and understood. Using a pragmatic constructivist case study, focus group and individual interviews were undertaken with 30 participants involved with university-funded interdisciplinary research teams. Findings illustrate that their iQoL was shaped by their capacity to engage in and achieve what they viewed as their core work and its outcomes. Related sub-themes addressed the social and relational climate, institutional environment and structure, and expectations and resources. We argue that further development of iQoL as a unique construct is required to adequately measure the full range of people’s experiences in academia, particularly when aiming to address ‘wicked’ social and global problems within a predominantly neoliberal context.
Mechanical Behavior of Circular Steel Tubular Beam-Columns Corroded Uniformly in Atmospheric Environment

Circular steel tube members with the absence of anticorrosive protection or coating failure are prone to uniform corrosion, which threatens the reliability and safety of members in the atmospheric environment. To fully study the mechanical behavior of uniformly corroded circular steel tubes, compression test and theoretical analysis were conducted, and two methods considering section reduction and material degradation, respectively, were adopted for the calculation of ultimate load carrying capacities of specimens. The results indicate that uniform corrosion did not change the failure modes of specimens, and all of them belonged to global buckling failure. The load carrying capacities and stiffness of specimens decreased with the increase of corrosion ratio, and the degree of reduction was greater than that of material degradation, showing a linear relationship with the corrosion rate. Under the same corrosion ratio, the specimens with larger eccentricity represented more obvious load carrying capacity and stiffness degradation. The load carrying capacities predicted by both methods were in good agreement with the test results and had a certain safety margin. The conservative degree of calculation results from three specifications followed a descending order of ANSI/AISC 360-16, GB 50017-2017, and EN 1993-1-1. Under the same corrosion ratio, the load carrying capacity variation of specimens between one-sided corrosion and two-sided corrosion was less than 3%.
A Temperature Error Parallel Processing Model for MEMS Gyroscope based on a Novel Fusion Algorithm

To deal with the influence of temperature drift for a Micro-Electro-Mechanical System (MEMS) gyroscope, this paper proposes a new temperature error parallel processing method based on a novel fusion algorithm. Firstly, immune based particle swarm optimization (IPSO) is employed for optimal parameters search for Variational Modal Decomposition (VMD). Then, we can get the optimal decomposition parameters, wherein permutation entropy (PE) is employed as the fitness function of the particles. Then, the improved VMD is performed on the output signal of the gyro to obtain intrinsic mode functions (IMFs). After judging by sample entropy (SE), the IMFs are divided into three categories: noise term, mixed term and feature term, which are processed differently. Filter the mixed term and compensate the feature term at the same time. Finally, reconstruct them and get the result. Compared with other optimization algorithms, IPSO has a stronger global search ability and faster convergence speed. After Back propagation neural network (BP) is enhanced by Adaptive boosting (Adaboost), it becomes a strong learner and a better model, which can approach the real value with higher precision. The experimental result shows that the novel parallel method proposed in this paper can effectively solve the problem of temperature errors.
Application of powdered activated carbon coated with zinc oxide nanoparticles prepared using a green synthesis in removal of Reactive Blue 19 and Reactive Black-5: adsorption isotherm and kinetic models

Dyes considered as a main environmental pollutant are present in various effluents released from different industries like textiles. Dyes cause problems such as reduced light penetration, allergies, and cancer when they are entered into the environment. The purpose of this study was to compare the efficiency of the activated carbon coated with the zinc oxide (ZnO) nanoparticles prepared by walnut shell in removing two dyes from aqueous environments. The study used a floating method to prepare the AC-ZnO composite. The composite structure and morphology were studied using Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller (BET), X-ray diffraction and field emission scanning electron microscopy techniques. The results confirmed the accuracy of the composite structure. Moreover, the study examined reaction time, solution pH, composite volume, and initial dye concentration and AC-ZnO composite recovery. The removal efficiencies under the optimal conditions for Reactive Blue 19 (RB-19) and Reactive Black-5 (RB-5) (dye concentrations 100 mg L–1, composite dose 1.5 g L–1, reaction time 45 min and pH 3) were 97.36% and 73.36%, respectively. The results of the experimental data were fitted well to the Langmuir isotherm, indicating monolayer adsorption of both metal ions onto the AC-ZnO composite and an estimated adsorptive capacity of 71.42 (RB-5) and 94.33 mg g–1 (RB-19). However, the kinetic data agreed well with the pseudo-second-order model. The SBET and total pure volume for the AC-ZnO composite and AC were 728.17 m2 g–1, 687.95 m2 g–1 and 0.684 cm3 g–1, 0.612 cm3 g–1, respectively. It can be concluded that AC-ZnO composite as an effective and environmentally friendly adsorbent had a high ability in removing dye from aqueous solutions.
SUSTAINABILITY REPORT PRACTICES IN INDONESIA: CONTEXT, POLICY, AND READABILITY

Considering sustainability disclosure become one important issue, while still there no universal agreement for the guidance, this study aims to examine Indonesia’s Sustainability Disclosure trend using GRI as quantity dimension, KLD for the quality, and readability and PROPER to measure how well they communicate. This study uses a sample of 224 firm-year observation from 2013-2017 based on GRI database. Descriptive analytic employed to figure disclosure trend in general, year to year, and industry base, and Pearson to explain correlation between measurements. There are three important issue discovered in this research. First, Indonesia’s sustainability disclosure is generally low but mixed among different proxies by the year, and SIC 2 as the best quantity disclosure and SIC 4 provides readable report. Second, we found a significant positive correlation between quantity and quality sustainability disclosure. Third, the result reveals an indication that PROPER award rely on corporate’s environmental risk disclosure. This study limited to public company that issue Sustainability Disclosure hence sample relatively small. This research provides insight for firms to improve quantity and quality of firm’s Sustainability Disclosure as development of trends is not optimized.
Assessing community organization needs for medical school Community Service-Learning

Objective While literature exists on student feedback to community service-learning (CSL), community organizations have played a small role in re-shaping CSL within medical curricula. The objective of this study was to analyze feedback from community supervisors involved in a CSL course at University of Torontos Faculty of Medicine. Methods Semi-structured one-to-one qualitative interviews were conducted with community supervisors (n = 9) between October 2018 and January 2019. Interviews were transcribed and thematic analysis was completed using Dedoose software. Researchers independently coded transcripts and developed an initial codebook. Once inter-rater reliability (Cohen's κ coefficient) > 0.80 was achieved, the remaining transcripts were coded deductively, and additional codes were developed inductively when existing code were unable to describe new data. Themes from the analysis were identified. Results Four overarching themes were identified. Perceived benefits to students by supervisors focused on their contributions to the development of future physicians through understanding community needs and exposure to organizations that may be resourceful to physicians. Among organizational benefits of CSL, extra hands, work-specific benefits, and medical students unique expertise were subthemes highlighted. CSL benefits were not without challenges. Scheduling difficulties, motivating students to understand their works value, and curriculum gaps identified were challenges supervisors faced. Three subthemes emerged under areas for improvement. Supervisors emphasized increasing flexibility to make experiences more meaningful for students. They also wanted more dialogue between organizations to gain insight from one another and a clearer understanding of CSL and its role in the curriculum. Conclusions Community supervisors viewed academic institutions to be in a unique position having established many partnerships with community organizations. Developing networking opportunities between organizations should be encouraged across CSL programs in medical education. As well, clearer understanding of CSL as part of the medical curriculum was important for supervisors to better understand their roles and responsibilities.
Variable screening for survival data in the presence of heterogeneous censoring

Variable screening for censored survival data is most challenging when both survival and censoring times are correlated with an ultrahigh‐dimensional vector of covariates. Existing approaches to handling censoring often make use of inverse probability weighting by assuming independent censoring with both survival time and covariates. This is a convenient but rather restrictive assumption which may be unmet in real applications, especially when the censoring mechanism is complex and the number of covariates is large. To accommodate heterogeneous (covariate‐dependent) censoring that is often present in high‐dimensional survival data, we propose a Gehan‐type rank screening method to select features that are relevant to the survival time. The method is invariant to monotone transformations of the response and of the predictors, and works robustly for a general class of survival models. We establish the sure screening property of the proposed methodology. Simulation studies and a lymphoma data analysis demonstrate its favorable performance and practical utility.