Miracle-dz/UniFAR-FTUs huggingface dataset:
DNA damage and oogenesis anomalies in Pimelia latreillei (Coleoptera: Tenebrionidae) induced by heavy metals soil pollution

The present study used Pimelia latreillei as a biomonitoring insect for heavy metals soil pollution in a populated industrial area at Zawya Abd El-Qader, Alexandria, Egypt. Comet assay and histological analysis were applied to evaluate the potential risk of heavy metals. X-ray analysis of the soil samples collected from the polluted site revealed significantly increased metal percentages compared with the reference site. Moreover, a significant increase in metal percentages was detected by the X-ray analysis in insect ovaries collected from the polluted site. The Tail DNA length was significantly greater in the insects collected from the polluted site—47.6% compared with 11.4% at the reference site. Pronounced disruptions in oogenesis were observed through histological and ultrastructure investigations in insects collected from the polluted site. The study summarized the potential utility of insect biomonitors in predicting the effect of heavy metals soil pollution on occupational health.
The Impact of p53 on Aristolochic Acid I-Induced Gene Expression In Vivo

Exposure to aristolochic acid (AA) is linked to kidney disease and urothelial cancer in humans. The major carcinogenic component of the AA plant extract is aristolochic acid I (AAI). The tumour suppressor p53 is frequently mutated in AA-induced tumours. We previously showed that p53 protects from AAI-induced renal proximal tubular injury, but the underlying mechanism(s) involved remain to be further explored. In the present study, we investigated the impact of p53 on AAI-induced gene expression by treating Trp53(+/+), Trp53(+/-), and Trp53(-/-) mice with 3.5 mg/kg body weight (bw) AAI daily for six days. The Clariom™ S Assay microarray was used to elucidate gene expression profiles in mouse kidneys after AAI treatment. Analyses in Qlucore Omics Explorer showed that gene expression in AAI-exposed kidneys is treatment-dependent. However, gene expression profiles did not segregate in a clear-cut manner according to Trp53 genotype, hence further investigations were performed by pathway analysis with MetaCore™. Several pathways were significantly altered to varying degrees for AAI-exposed kidneys. Apoptotic pathways were modulated in Trp53(+/+) kidneys; whereas oncogenic and pro-survival pathways were significantly altered for Trp53(+/-) and Trp53(-/-) kidneys, respectively. Alterations of biological processes by AAI in mouse kidneys could explain the mechanisms by which p53 protects from or p53 loss drives AAI-induced renal injury in vivo.
Modulation of Extracellular Matrix Rigidity Via Riboflavin‐mediated Photocrosslinking Regulates Invasive Motility and Treatment Response in a 3D Pancreatic Tumor Model

In this study, we evaluate the use of riboflavin‐mediated collagen photocrosslinking as an experimental tool to modulate extracellular matrix (ECM) mechanical properties in 3D in vitro tumor models. Using this approach in conjunction with 3D pancreatic tumor spheroid transplants, we show that the extent of matrix photocrosslinking in reconstituted hydrogels with fixed protein concentration scales inversely with the extent of invasive progression achieved by cells infiltrating into the surrounding ECM from primary transplanted spheroids. Using cross‐linking to manipulate the extent of invasion into ECM in conjunction with imaging‐based treatment assessment, we further leverage this approach as a means for assaying differential therapeutic response in primary nodule and ECM‐invading populations and compare response to verteporfin‐based photodynamic therapy (PDT) and oxaliplatin chemotherapy. Treatment response data shows that invading cell populations (which also exhibit markers of increased EMT) are highly chemoresistant yet have significantly increased sensitivity to PDT relative to the primary nodule. In contrast, the oxaliplatin treatment achieves greater growth inhibition of the primary nodule. These findings may be significant in themselves, while the methodology developed here could have a broader range of applications in developing strategies to target invasive disease and/or mecahanobiological determinants of therapeutic response in solid tumors.
Proposal for an Embedded System Architecture Using a GNDVI Algorithm to Support UAV-Based Agrochemical Spraying

An important area in precision agriculture is related to the efficient use of chemicals applied onto fields. Efforts have been made to diminish their use, aiming at cost reduction and fewer chemical residues in the final agricultural products. The use of unmanned aerial vehicles (UAVs) presents itself as an attractive and cheap alternative for spraying pesticides and fertilizers compared to conventional mass spraying performed by ordinary manned aircraft. Besides being cheaper than manned aircraft, small UAVs are capable of performing fine-grained instead of the mass spraying. Observing this improved method, this paper reports the design of an embedded real-time UAV spraying control system supported by onboard image processing. The proposal uses a normalized difference vegetation index (NDVI) algorithm to detect the exact locations in which the chemicals are needed. Using this information, the automated spraying control system performs punctual applications while the UAV navigates over the crops. The system architecture is designed to run on low-cost hardware, which demands an efficient NDVI algorithm. The experiments were conducted using Raspberry Pi 3 as the embedded hardware. First, experiments in a laboratory were conducted in which the algorithm was proved to be correct and efficient. Then, field tests in real conditions were conducted for validation purposes. These validation tests were performed in an agronomic research station with the Raspberry hardware integrated into a UAV flying over a field of crops. The average CPU usage was about 20% while memory consumption was about 70 MB for high definition images, with 4% CPU usage and 20.3 MB RAM being observed for low-resolution images. The average current measured to execute the proposed algorithm was 0.11 A. The obtained results prove that the proposed solution is efficient in terms of processing and energy consumption when used in embedded hardware and provides measurements which are coherent with the commercial GreenSeeker equipment.
Microstructural analysis of skeletal muscle force generation during aging

Human aging results in a progressive decline in the active force generation capability of skeletal muscle. While many factors related to the changes of morphological and structural properties in muscle fibers and the extracellular matrix (ECM) have been considered as possible reasons for causing age‐related force reduction, it is still not fully understood why the decrease in force generation under eccentric contraction (lengthening) is much less than that under concentric contraction (shortening). Biomechanically, it was observed that connective tissues (endomysium) stiffen as ages, and the volume ratio of connective tissues exhibits an age‐related increase. However, limited skeletal muscle models take into account the microstructural characteristics as well as the volume fraction of tissue material. This study aims to provide a numerical investigation in which the muscle fibers and the ECM are explicitly represented to allow quantitative assessment of the age‐related force reduction mechanism. To this end, a fiber‐level honeycomb‐like microstructure is constructed and modeled by a pixel‐based Reproducing Kernel Particle Method (RKPM), which allows modeling of smooth transition in biomaterial properties across material interfaces. The numerical investigation reveals that the increased stiffness of the passive materials of muscle tissue reduces the force generation capability under concentric contraction while maintains the force generation capability under eccentric contraction. The proposed RKPM microscopic model provides effective means for the cellular‐scale numerical investigation of skeletal muscle physiology.
AtSIBP1, a Novel BTB Domain-Containing Protein, Positively Regulates Salt Signaling in Arabidopsis thaliana

Because they are sessile organisms, plants need rapid and finely tuned signaling pathways to adapt to adverse environments, including salt stress. In this study, we identified a gene named Arabidopsis thaliana stress-induced BTB protein 1 (AtSIBP1), which encodes a nucleus protein with a BTB domain in its C-terminal side and is induced by salt and other stresses. The expression of the β-glucuronidase (GUS) gene driven by the AtSIBP1 promoter was found to be significantly induced in the presence of NaCl. The sibp1 mutant that lost AtSIBP1 function was found to be highly sensitive to salt stress and more vulnerable to salt stress than the wild type WT, while the overexpression of AtSIBP1 transgenic plants exhibited more tolerance to salt stress. According to the DAB staining, the sibp1 mutant accumulated more reactive oxygen species (ROS) than the WT and AtSIBP1 overexpression plants after salt stress. In addition, the expression levels of stress-induced marker genes in AtSIBP1 overexpression plants were markedly higher than those in the WT and sibp1 mutant plants. Therefore, our results demonstrate that AtSIBP1 was a positive regulator in salinity responses in Arabidopsis.
Greater Lower Limb Fatigability in People with Prediabetes than Controls

ABSTRACT Introduction The study purpose was to compare perceived fatigability and performance fatigability after high-velocity contractions with knee extensor muscles between people with prediabetes, people with type 2 diabetes (T2D), and controls without diabetes matched for age, body mass index, and physical activity. Methods Twenty people with prediabetes (11 men, 9 women: 63.1 ± 6.0 yr, 26.9 ± 4.2 kg·m−2, 8030 ± 3110 steps per day), 39 with T2D (23 men, 16 women: 61.2 ± 8.5 yr, 29.4 ± 6.4 kg·m−2, 8440 ± 4220 steps per day), and 27 controls (13 men, 14 women: 58.1 ± 9.4 yr, 27.3 ± 4.3 kg·m−2, 8400 ± 3000 steps per day) completed the Fatigue Impact Scale as a measure of perceived fatigability and a fatigue protocol including 120 maximal-effort, high-velocity concentric contractions (MVCC; 1 contraction/3 s) with the knee extensors using a submaximal load (30% maximum) to quantify performance fatigability. Electrical stimulation was used to assess voluntary activation and contractile function of the knee extensor muscles before and after the fatigue protocol. Results Fatigue Impact Scale scores were not different between people with prediabetes, people with T2D, and controls (12.5 ± 15.1, 18.3 ± 22.7, and 12.6 ± 18.6, respectively; P = 0.517). However, people with prediabetes had greater reductions in MVCC power during the fatigue protocol than did controls (31.8% ± 22.6% vs 22.1% ± 21.1%, P < 0.001), and both groups had lesser reductions than the T2D group (44.8% ± 21.9%, P < 0.001). Similarly, the prediabetes group had larger reductions in electrically evoked twitch amplitude than the control group (32.5% ± 24.9% vs 21.3% ± 33.0%, P < 0.001), but lesser reductions than those with T2D (44.0% ± 23.4%, P < 0.001). For all three groups, a greater decline in MVCC power was associated with larger reductions of twitch amplitude (r2 = 0.350, P < 0.001). Conclusion People with prediabetes have greater performance fatigability of the knee extensors due to contractile mechanisms compared with controls, although less performance fatigability than that of people with T2D.
Development of the brainstem respiratory circuit

The respiratory circuit is comprised of over a dozen functionally and anatomically segregated brainstem nuclei that work together to control respiratory rhythms. These respiratory rhythms emerge prenatally but only acquire vital importance at birth, which is the first time the respiratory circuit faces the sole responsibility for O2/CO2 homeostasis. Hence, the respiratory circuit has little room for trial‐and‐error‐dependent fine tuning and relies on a detailed genetic blueprint for development. This blueprint is provided by transcription factors that have specific spatiotemporal expression patterns along the rostral‐caudal or dorsal‐ventral axis of the developing brainstem, in proliferating precursor cells and postmitotic neurons. Studying these transcription factors in mice has provided key insights into the functional segregation of respiratory control and the vital importance of specific respiratory nuclei. Many studies converge on just two respiratory nuclei that each have rhythmogenic properties during the prenatal period: the preBötzinger complex (preBötC) and retrotrapezoid nucleus/parafacial nucleus (RTN/pF). Here, we discuss the transcriptional regulation that guides the development of these nuclei. We also summarize evidence showing that normal preBötC development is necessary for neonatal survival, and that neither the preBötC nor the RTN/pF alone is sufficient to sustain normal postnatal respiratory rhythms. Last, we highlight several studies that use intersectional genetics to assess the necessity of transcription factors only in subregions of their expression domain. These studies independently demonstrate that lack of RTN/pF neurons weakens the respiratory circuit, yet these neurons are not necessary for neonatal survival because developmentally related populations can compensate for abnormal RTN/pF function at birth.
Preparation of levofloxacin loaded in situ gel for sustained ocular delivery: in vitro and ex vivo evaluations

Abstract The major drawback of the eye drops is rapid elimination of drug from the precorneal region, thus ensuing poor bioavailability as well as therapeutic efficacy. To conquer these limitations, a pH triggered in situ gel was developed for sustained delivery of levofloxacin. Two polymers namely hydroxypropyl methylcellulose (HPMC) and sodium alginate along with the boric acid buffer were used to formulate the in situ gel. Based on the various physicochemical evaluation parameters like pH, clarity and gelling capacity placebo formulations were selected and further characterized for viscosity, in vitro release, ex vivo corneal permeation, and histopathological studies. The optimized in situ gel (F28) showed sustained release of 93 ± 4.23% for 24 h and cumulative drug permeation of 71.81 ± 4.7% for 72 h. Additionally, ocular irritation study and histopathology of the formulation treated cornea confirm the non-irritancy of the optimized formulation. Based on all the above performed studies, it can be concluded that the in situ gel would present a fruitful alternative for the ocular infections.
No More Bricks in the Wall: Adopting Healthy Lifestyles through Physical Education Classes

Despite the multiple benefits associated with practicing physical activity regularly, less than 20% of the population do it on a daily basis. Physical education classes could contribute, during childhood and adolescence, to consolidating adherence to healthy lifestyle habits. The present study involved 606 secondary school students between the ages of 13 and 19. We analysed the relationships between the perception of psychological control and support for autonomy, the satisfaction and frustration of psychological needs, mind-wandering and mindfulness, positive and negative emotions, motivation towards physical education classes, physical activity and the intention to be physically active—all through a structural equation model, which presented acceptable goodness-of-fit indices. The results showed that students who feel more autonomous see that their psychological needs are met and feel emotionally positive; this will result in the development of autonomous motivation towards physical education classes and physical activity that, in turn, could lead to a greater intention to be physically active.
Effect of Faint Road Traffic Noise Mixed in Birdsong on the Perceived Restorativeness and Listeners’ Physiological Response: An Exploratory Study

Many studies have reported that natural sounds (e.g., birdsong) are more restorative than urban noise. These studies have used physiological and psychological indicators, such as the skin conductance level (SCL) and the Perceived Restorativeness Scale (PRS), to evaluate the restorative effect of natural sounds. However, the effect of faint background noise mixed with birdsong on the restorativeness of birdsong has not been described yet. In the current experiment, we examined whether traffic noise affects the perceived restorativeness and the physiological restorativeness of birdsong in a low-stress condition using the SCL and the PRS. The scores of the PRS showed that birdsong significantly increased the perceived restorativeness of the place regardless of the car noise, but no significant difference was found between these two birdsongs. In contrast, physiologically, the birdsong without car noise decreased the participants’ SCL significantly more than the birdsong with car noise did. These results indicate that the SCL would be useful to detect the effect of background noise on natural sound when the noise is too low to affect the perceived restorativeness. This study highlights the importance of measuring the SCL besides assessing perceived restorativeness to describe the characteristics of restorative natural sound in future research.
Antibody validation for Western blot: By the user, for the user

Well-characterized antibody reagents play a key role in the reproducibility of research findings, and inconsistent antibody performance leads to variability in Western blotting and other immunoassays. The current lack of clear, accepted standards for antibody validation and reporting of experimental details contributes to this problem. Because the performance of primary antibodies is strongly influenced by assay context, recommendations for validation and usage are unique to each type of immunoassay. Practical strategies are proposed for the validation of primary antibody specificity, selectivity, and reproducibility using Western blot analysis. The antibody should produce reproducible results within and between Western blotting experiments and the observed effect confirmed with a complementary or orthogonal method. Routine implementation of standardized antibody validation and reporting in immunoassays such as Western blotting may promote improved reproducibility across the global life sciences community.
New morphological evidence of the ‘fate’ of growth plate hypertrophic chondrocytes in the general context of endochondral ossification

The ‘fate’ of growth plate hypertrophic chondrocytes has been long debated with two opposing theories: cell apoptosis or survival with transformation into osteogenic cells. This study was carried out on the proximal tibial growth plate of rabbits using light microscopy, scanning and transmission electron microscopy. We focused particularly on the orientation of the specimens included in order to define the mineral deposition and the vascular invasion lines and obtain histological and ultrastructural images at the corresponding height of the plate. Chondrocyte morphology transformation through the maturation process (characterized by vesicles and then large cytoplasmic lacunae before condensation, fragmentation and disappearance of the nuclear chromatin) did not correspond to that observed in the ‘in vitro’ apoptosis models. These findings rather suggested the passage of free water from the cartilage matrix into a still live cell (swelling). The level of these changes suggested a close relationship with the mineral deposition line. Furthermore, the study provided evidence that the metaphyseal capillaries could advance inside the columns of stacked hypertrophic chondrocytes (delimited by the intercolumnar septa) without the need for calcified matrix resorption because the thin transverse septa between the stacked chondrocyte (below the mineral deposition line) were not calcified. The zonal distribution of cell types (hypertrophic chondrocytes, osteoblasts, osteoclasts and macrophages) did not reveal osteoclasts or chondroclasts at this level. Morphological and morphometric analysis recorded globular masses of an amorphous, necrotic material in a zone 0–70 μm below the vascular invasion line occasionally surrounded by a membrane (indicated as ‘hypertrophic chondrocyte ghosts’). These masses and the same material not bound by a membrane were surrounded by a large number of macrophages and other blood cell precursors, suggesting this could be the cause of macrophage recall and activation. The most recent hypotheses based on genetic and lineage tracing studies stating that hypertrophic chondrocytes can survive and transform into osteoblasts and osteocytes (trans‐differentiation) were not confirmed by the ultrastructural morphology or by the zonal comparative counting and distribution of cell types below the vascular invasion line.
Longitudinal investigation on personality traits and mental health relationships: the mediating role of work-family interference and enhancement

results The results indicate that conscientiousness and emotional stability are the personality traits that are associated with work-to-family enhancement over time. In addition, workto-family enhancement is a mediator between emotional stability and mental health. Overall, there exists a positive reciprocal relationship between work-to-family enhancement and mental health throughout the three waves. conclusions This study showed that there is a bidirectional positive link between work-to-family enhancement and mental health. The findings suggest that organizations will benefit not only from developing interventions to enrich employees’ work skills, behaviors/attitudes but also from promoting their mental health and emotional stability. This study is the first to confirm the partial mediation role of workto-family enhancement between emotional stability and mental health, and bidirectional relations of work-to-family enhancement and mental health. Moreover, the longitudinal design of this study overcomes the limitations of prior cross-sectional studies in evaluating the relationships of constructs.
BANANAS: Bayesian Optimization with Neural Networks for Neural Architecture Search

Neural Architecture Search (NAS) has seen an explosion of research in the past few years. A variety of methods have been proposed to perform NAS, including reinforcement learning, Bayesian optimization with a Gaussian process model, evolutionary search, and gradient descent. In this work, we design a NAS algorithm that performs Bayesian optimization using a neural network model. We develop a path-based encoding scheme to featurize the neural architectures that are used to train the neural network model. This strategy is particularly effective for encoding architectures in cell-based search spaces. After training on just 200 random neural architectures, we are able to predict the validation accuracy of a new architecture to within one percent of its true accuracy on average. This may be of independent interest beyond Bayesian neural architecture search. We test our algorithm on the NASBench dataset (Ying et al. 2019), and show that our algorithm significantly outperforms other NAS methods including evolutionary search, reinforcement learning, and AlphaX (Wang et al. 2019). Our algorithm is over 100x more efficient than random search, and 3.8x more efficient than the next-best algorithm. We also test our algorithm on the search space used in DARTS (Liu et al. 2018), and show that our algorithm is competitive with state-of-the-art NAS algorithms on this search space.