Miracle-dz/UniFAR-FTUs huggingface dataset:
Vitamin D and Indices of Bone and Carbohydrate Metabolism in Postmenopausal Women Subjected to a 12-Week Aerobic Training Program—The Pilot Study

The purpose of this study was to assess the effect of Nordic walking training on the indices of bone and carbohydrate metabolism in relation to 25(OH)D levels in postmenopausal women that were subjected to the outdoor systematic physical activity. The study was performed in 10 postmenopausal women, who participated in a 12-week Nordic walking exercise program, taking place during spring months (March to June). Anthropometric and biochemical parameters were measured before and after the training program. Serum concentrations of 25-hydroksycholekalciferol (25(OH)D), parathyroid hormone (PTH), insulin, glucose, osteocalcin (OC), C-terminal telopeptide of type I collagen (CTX), and calcium were determined. After the Nordic walking exercise program, a significant increase in the serum levels of 25(OH)D and CTX and a decrease in body mass, body mass index (BMI), fat mass, and PTH concentrations were observed. The findings of the present study suggest that 25(OH)D, as important metabolic regulator, plays a role in the modification of bone markers’ responses after the outdoor training program, independent of the physical activity effects.
PDMS Based Hybrid Sol-Gel Materials for Sensing Applications in Alkaline Environments: Synthesis and Characterization

Nowadays, concrete degradation is a major problem in the civil engineering field. Concrete carbonation, one of the main sources of structures’ degradation, causes concrete’s pH to decrease; hence, enabling the necessary conditions for corrosion reinforcement. An accurate, non-destructive sensor able to monitor the pH decrease resistant to concrete conditions is envisaged by many researchers. Optical fibre sensors (OFS) are generally used for concrete applications due to their high sensitivity and resistance to external interferences. Organic-inorganic hybrid (OIH) films, for potential functionalization of OFS to be applied in concrete structures, were developed. Polydimethylsiloxane (PDMS) based sol-gel materials were synthesized by the formation of an amino alcohol precursor followed by hydrolysis and condensation. Different ratios between PDMS and (3-aminopropyl)triethoxysilane (3-APTES) were studied. The synthesized OIH films were characterized by Fourier-transformed infrared spectroscopy (FTIR), UV–Vis spectroscopy, electrochemical impedance spectroscopy (EIS) and thermogravimetric analysis (TGA). The OIH films were doped with phenolphthalein (Phph), a pH indicator, and were characterized by UV–Vis and EIS. FTIR characterization showed that the reaction between both precursors, the hydrolysis and the condensation reactions occurred successfully. UV–Vis characterization confirmed the presence of Phph embedded in the OIH matrices. Dielectric and thermal properties of the materials showed promising properties for application in contact with a high alkaline environment.
Retirement anxiety and depressive symptoms among middle-aged adults: An indirect effect through death anxiety

Abstract Basing our argument on Terror Management Theory, we posit that retirement is an age-related transition, which could result in greater death saliency and anxiety, leading to increased depressive symptoms. An indirect-effect model was tested on a convenience sample of 574 Israeli Jewish older workers (mean age = 57), finding that the link between retirement anxiety and depressive symptoms is mediated through death anxiety. Anxious anticipation of retirement could imply that the individual perceives it as an “end,” resulting in greater death saliency and death anxiety, which are related to poorer mental health. Theoretical and practical implications are discussed.
Integrating Minority Stress Theory and the Interpersonal Theory of Suicide among Sexual Minority Youth Who Engage Crisis Services.

OBJECTIVE The present study sought to integrate minority stress theory (MST) and the interpersonal theory of suicide (ITS) to better understand high rates of suicide among sexual minority youth (SMY). To date, the ITS and MST have largely advanced independently from one another even though the research base for each theory contains gaps that the other theory may help fill. METHOD Using data from a national sample of 564 SMY (aged 12-24) recruited from an LGBTQ youth-focused suicide crisis prevention provider, we examined structural equation models to understand how perceived burdensomeness and thwarted belongingness mediate the relationship between minority stress and suicidal ideation and attempt. RESULTS Sexual minority stress was significantly associated with both perceived burdensomeness and thwarted belongingness in models predicting suicidal ideation and attempt. Moreover, minority stress had a direct effect on suicide attempt and an indirect effect on both suicidal ideation and suicide attempt through burdensomeness. CONCLUSIONS Given that minority stress is associated with greater thwarted belongingness, perceived burdensomeness, and suicide attempts, there should be greater demand for continuing education centered on sexual minority populations and population-specific services. Identifying burdensomeness as a minority stress-suicide mechanism highlights the potential gains of piloting recently developed burdensomeness interventions among SMY.
Effectiveness of clinical surface cleaning and disinfection: evaluation methods.

OBJECTIVE To discuss the methods employed to evaluate the effectiveness of clinical surface cleaning and disinfection (C&D). METHOD This is a theoretical reflection based on scientific studies and the experience of the authors. Knowledge and current gaps, the need for further studies, and practical application of the methods were approached. RESULTS There are four main methods used to evaluate the effectiveness of clinical surface C&D: visual inspection, fluorescent markers, microbiological cultures, and adenosine triphosphate (ATP) bioluminescence. The first two are used to evaluate the process and to predict adherence to protocols by the staff, and the last two are employed to evaluate the results, therefore being the most relevant to assess the risk of infection. FINAL CONSIDERATIONS The ideal method was not found, because all of them showed limitations. There is a need for strategies to optimize the precision of these methods.
Successful treatment of cholesterol crystal embolism with anti-proprotein convertase subtilisin/kexin type 9 (PCSK9) antibody: a case report

Abstract Background We report a unique case of renal cholesterol crystal embolism (CCE) induced by carotid artery stenting that was successfully treated with evolocumab, a fully human monoclonal antibody against proprotein convertase subtilisin kexin type 9 (PCSK9). Case presentation A 77-year-old man with hypertension, hyperlipidemia, and chronic kidney disease was referred to our department for decreased estimated glomerular filtration rate (eGFR)—from 32.0 to 13.9 mL/min/1.73 m2—5 weeks after carotid artery stenting. Further examination revealed livedo reticularis in the bilateral toes and eosinophilia (723/μL). Skin biopsy from livedo reticularis tissue in the bilateral toes showed cholesterol clefts in the small arteries. The patient was therefore diagnosed with CCE. After 25 weeks’ administration of evolocumab at a dose of 140 mg subcutaneously administered every 2 weeks, his eGFR had improved from 10.7 to 18.1 mL/min/1.73 m2. Conclusion Evolocumab may have a beneficial effect on renal involvement in patients with CCE.
Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress–induced senescence

Oxidative stress-induced DNA damage, the senescence-associated secretory phenotype (SASP), and impaired autophagy all are general features of senescent cells. However, the cross-talk among these events and processes is not fully understood. Here, using NIH3T3 cells exposed to hydrogen peroxide stress, we show that stress-induced DNA damage provokes the SASP largely via cytosolic chromatin fragment (CCF) formation, which activates a cascade comprising cGMP-AMP synthase (cGAS), stimulator of interferon genes protein (STING), NF-κB, and SASP, and that autolysosomal function inhibits this cascade. We found that CCFs accumulate in senescent cells with activated cGAS-STING-NF-κB signaling, promoting SASP and cellular senescence. We also present evidence that the persistent accumulation of CCFs in prematurely senescent cells is partially associated with a defect in DNA-degrading activity in autolysosomes and reduced abundance of activated DNase 2α. Intriguingly, we found that metformin- or rapamycin-induced activation of autophagy significantly lessened the size and levels of CCFs and repressed the activation of the cGAS-STING-NF-κB-SASP cascade and cellular senescence. These effects of autophagy activators indicated that autolysosomal function contributes to CCF clearance and SASP suppression, further supported by the fact that the lysosome inhibitor bafilomycin A1 blocked the role of autophagy-mediated CCF clearance and senescence repression.
Haemoparasitic Infections in Cattle from a Trypanosoma brucei Rhodesiense Sleeping Sickness Endemic District of Eastern Uganda

We carried out a baseline survey of cattle in Kaberamaido district, in the context of controlling the domestic animal reservoir of Trypanosoma brucei rhodesiense human African trypanosomiasis (rHAT) towards elimination. Cattle blood was subjected to capillary tube centrifugation followed by measurement of the packed cell volume (PCV) and examination of the buffy coat area for motile trypanosomes. Trypanosomes were detected in 561 (21.4%) out of 2621 cattle screened by microscopy. These 561 in addition to 724 apparently trypanosome negative samples with low PCVs (≤25%) were transported to the laboratory and tested by PCR targeting the trypanosomal Internal Transcribed Spacer (ITS-1) as well as suspect Tick-Borne Diseases (TBDs) including Anaplasmamosis, Babesiosis, and Theileriosis. PCR for Anaplasma sp yielded the highest number of positive animals (45.2%), followed by Trypanosoma sp (44%), Theileria sp (42.4%) and Babesia (26.3%); multiple infections were a common occurrence. Interestingly, 373 (29%) of these cattle with low PCVs were negative by PCR, pointing to other possible causes of aneamia, such as helminthiasis. Among the trypanosome infections classified as T. brucei by ITS-PCR, 5.5% were positive by SRA PCR, and were, therefore, confirmed as T. b. rhodesiense. Efforts against HAT should therefore consider packages that address a range of conditions. This may enhance acceptability and participation of livestock keepers in programs to eliminate this important but neglected tropical disease. In addition, we demonstrated that cattle remain an eminent reservoir for T. b. rhodesiense in eastern Uganda, which must be addressed to sustain HAT elimination.
Image domain multi-material decomposition using single energy CT

Multi-material decomposition (MMD) technique decomposes the CT images into basis material images and has been promising in clinical practice for material composition quantification within the human body. MMD could be implemented using the image data acquired from spectral CT or its special case, dual-energy CT (DECT) while the spectral CT data acquisition usually requires a hardware modification. In this paper, we propose an image domain MMD method using single energy CT (SECT). The proposed objective function applies a least square data fidelity term to enforce the minimization between the linear combination of decomposed material image and the measured SECT image, and an edge-preserving (EP) regularization term to meet the piecewise constant property of the material image. We apply the optimization transfer principle to form a pixel-wise separable quadratic surrogate (PWSQS) function in each iteration to decrease the objective function. The pixelwise direct inversion method assisted by the two-material assumption (TMA) is applied to obtain a good initial value. The proposed method is evaluated using a digital phantom, a Catphan phantom and the clinical data. A low-pass filtration method is implemented for a comparison purpose. In the phantom study, the proposed TMA method achieves high volume fraction accuracy (VFA) of 79.64% and the proposed EP method further increases the VFA by 15.56% and decreases the decomposition standard deviation (STD) by 81.51% compared with the TMA method. At the comparable noise level, the proposed EP method increases spatial resolution by an overall factor of 1.01 when the modulation transfer function magnitude is decreased to 50% compared with the low-pass filtration method. In clinical data study, the virtual non-contrast image generated by the proposed method achieves the root-mean-squared-relative error of 2.93% compared with the contrast-free ground-truth image.
Identification and Heterologous Expression of the Biosynthetic Gene Cluster Encoding the Lasso Peptide Humidimycin, a Caspofungin Activity Potentiator

Humidimycin (MDN-0010) is a ribosomally synthesized and post-translationally modified peptide (RiPP) belonging to class I lasso peptides, and is structurally related to siamycins, which have been shown to have strong antimicrobial activities against Gram-positive bacteria and to possess anti-HIV activity. Humidimycin was isolated from the strain Streptomyces humidus CA-100629, and was shown to synergize the activity of the fungal cell wall inhibitor caspofungin. In this work, the biosynthetic gene cluster of humidimycin was identified by genome mining of S. humidus CA-100629, cloned by Gibson assembly, and heterologously expressed.
Morphological, Pathogenic and Toxigenic Variability in the Rice Sheath Rot Pathogen Sarocladium Oryzae

Sheath rot is an emerging rice disease that leads to considerable yield losses. The main causal agent is the fungus Sarocladium oryzae. This pathogen is known to produce the toxins cerulenin and helvolic acid, but their role in pathogenicity has not been clearly established. S. oryzea isolates from different rice-producing regions can be grouped into three phylogenetic lineages. When grown in vitro, isolates from these lineages differed in growth rate, colour and in the ability to form sectors. A diverse selection of isolates from Rwanda and Nigeria, representing these lineages, were used to further study their pathogenicity and toxin production. Liquid chromatography high-resolution mass spectrometry analysis was used to measure cerulenin and helvolic acid production in vitro and in planta. The three lineages clearly differed in pathogenicity on the japonica cultivar Kitaake. Isolates from the least pathogenic lineage produced the highest levels of cerulenin in vitro. Helvolic acid production was not correlated with the lineage. Sectorisation was observed in isolates from the two least pathogenic lineages and resulted in a loss of helvolic acid production. In planta, only the production of helvolic acid, but not of cerulenin, correlated strongly with disease severity. The most pathogenic isolates all belonged to one lineage. They were phenotypically stable, shown by the lack of sectorisation, and therefore maintained high helvolic acid production in planta.
Effects of Anchomanes difformis on Inflammation, Apoptosis, and Organ Toxicity in STZ-Induced Diabetic Cardiomyopathy

Persistent hyperglycemia is known to cause enhanced generation of reactive oxygen species in diabetes. Several inflammatory cytokines are induced by oxidative stress, and their release also leads to increased oxidative stress; this makes oxidative stress one of the important factors in the development of chronic inflammation and other immune responses. These have been implicated in the development of diabetic complications such as nephropathy and cardiomyopathy. Anchomanes difformis has been shown to possess antioxidant and anti-inflammatory potentials. The present study investigated the immunomodulatory potential and the antiapoptotic ability of Anchomanes difformis to ameliorate heart toxicity and injury in type II diabetes. Two weeks of fructose (10%) administration followed by single intraperitoneal injection of streptozotocin (40 mg/kg) were used to induce type II diabetes in male Wistar rats. Leaf extract (aqueous) of Anchomanes difformis (200 and 400 mg/kg) was administered orally for six weeks. Blood glucose concentrations and body weights before and after interventions were determined. Interleukin (IL)-1β, IL-6, IL-10, IL-18, monocyte chemoattractant protein 1 (MCP-1), and tumor necrosis factor alpha (TNFα) were measured in the heart homogenates. Catalase (CAT), superoxide dismutase (SOD), total protein, oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS), and heart-type fatty acid-binding protein (H-FABP) levels were determined. Expressions of transcription factors (Nrf 2 and NFkB/p65) and apoptotic markers were also investigated in the heart. Anchomanes difformis administration reduced pro-inflammatory cytokines, increased anti-inflammatory markers, and enhanced antioxidant defense in the heart of diabetic treated animals. Anchomanes difformis is a new, promising therapeutic agent that can be explored for the treatment of pathological conditions associated with immune responses and will be a useful tool in the management of associated diabetic complications.
Collaborative Modeling of Group Learning Applications Using Eclipse Technology

The design and creation of groupware tools is a complex task that usually requires the participation of different stakeholders (software engineers, designers, etc.), either working at the same time or collaborating asynchronously. This paper describes an innovative model-driven development process to support the collaborative modeling of group learning applications, as well as the Computer Aided Software Engineering (CASE) tool that technologically supports it, the Learning Collaborative Interactive Applications Tool (Learn-CIAT) graphical editor. In its development, we applied technologies integrated within the Eclipse platform. The processes and tools described in this paper supply an important contribution to systematize the design and development of these kinds of applications.
Increasing of rumen fermentation products and digestibility of diets through urea-zeolite supplementation in-vitro

The aim of this study was to find the optimal level of urea-zeolite supplementation in the substrate on rumen fermentation products and digestion in vitro. Testing the solubility level of urea-zeolite at a ratio of 1: 1 and 1: 2 in rumen fluid based on N-NH3 levels was carried out at the fermentation time of 0, 1, 2, 3, 4, and 5 hours. Completely randomized design was used in this research with urea-zeolite supplementation treatment (0.2, 4, 6, 8, and 10% of dry matter (DM) substrate). Substrate composed of corn sugarcane silage and concentrate with DM ratio was 70%: 30%. The variables measured were rumen fermentation products (pH, VFA, and N-NH3), DM and organic matter (OM) digestibility. The results showed that the solubility of urea-zeolite in rumen fluid at the same ratio of 1: 1 and 1: 2, the highest at 4 hours fermentation time of 40.81% and 39.13% and after 4 hours was stable. Urea-zeolite supplementation (ratio 1: 2) on the substrate has no effect (P> 0.01) on the pH of the liquid. N-NH3 fermentation products increased (P <0.01) linearly with increasing levels of urea-zeolite supplementation. The total VFA product and the degradation of dry matter were highly significant (P <0.01) influenced by urea-zeolite supplementation and were optimal at 6% and 4% DM substrate supplementation, respectively. DM and OM digestibility was highly significant (P <0.01) influenced by urea-zeolite supplementation and was optimal at the 6% BK substrate level. It was concluded that the use of urea-zeolite as a supplement in the substrate could increase fermentation products and digestibility in vitro
CPT-Based Probabilistic Characterization of Undrained Shear Strength of Clay

Applying random field theory involves two important issues: the statistical homogeneity (or stationarity) and determination of random field parameters and correlation function. However, the profiles of soil properties are typically assumed to be statistically homogeneous or stationary without rigorous statistical verification. It is also a challenging task to simultaneously determine random field parameters and the correlation function due to a limited amount of direct test data and various uncertainties (e.g., transformation uncertainties) arising during site investigation. This paper presents Bayesian approaches for probabilistic characterization of undrained shear strength using cone penetration test (CPT) data and prior information. Homogeneous soil units are first identified using CPT data and subsequently assessed for weak stationarity by the modified Bartlett test to reject the null hypothesis of stationarity. Then, Bayesian approaches are developed to determine the random field parameters and simultaneously select the most probable correlation function among a pool of candidate correlation functions within the identified statistically homogeneous layers. The proposed approaches are illustrated using CPT data at a clay site in Shanghai, China. It is shown that Bayesian approaches provide a rational tool for proper determination of random field model for probabilistic characterization of undrained shear strength with consideration of transformation uncertainty.
JOURNAL OF RURAL COOPERATION

Insufficient access to rural credit institutions, the absence of marketing chains for agricultural products and limited application of advanced farming technology are the main development constraints in the Georgian agricultural sector. To research farmers’ demand for rural financial services a representative household survey was conducted early 2008 in the region of Shida Kartli (N=406). The survey included a choice experiment to investigate farmer’s preferences for characteristics of particular credit schemes. Results show that segments of the farmer population differ in their preferences for loan attributes. Furthermore, farmers expressed a very high demand for small credits with individual liability, and one third of them have experience with loans. These findings provide useful information for future credit cooperatives. Barriers to implementation are lack of trust among farmers and misconception of cooperatives with the former Soviet kolkhozes. Information campaigns can therefore be a key ingredient for the successful establishment of credit cooperatives. 1 Klaus Glenk is with the Macaulay Institute and Associated Companies, Aberdeen, United Kingdom (E-mail: k.glenk@macaulay.ac.uk). Johanna Pavliashvili (Corresponding author), is with the Georg-August Universität Göttingen, Department of Agricultural Economics and Rural Development, Göttingen, Germany (E-mail: jpavlia@gwdg.de). Adriano Profeta is with Technische Universität München, Freising, Germany (E-Mail: adriano.profeta@wzw.tum.de). JOURNAL OF RURAL COOPERATION, 37(1) 2009: 72–87 © ISSN 0377–7480 Preferences for rural credit systems and their impact 73
N ov 2 01 8 Advanced statistical methods to fit nuclear models

We discuss advanced statistical methods to improve parameter estimation of nuclear models. In particular, using the Liquid Drop Model for nuclear binding energies, we show that the area around the global χ minimum can be efficiently identified using Gaussian Process Emulation. We also demonstrate how Markov-chain Monte-Carlo sampling is a valuable tool for visualising and analysing the associated multidimensional likelihood surface.