Miracle-dz/UniFAR-FTUs huggingface dataset:
JGP_201711878 1065..1090

1065 Introduction The release of Ca ions from intracellular membrane-bound stores is a key step in a wide variety of biological functions. The release of Ca is predominantly mediated by two related Ca release channel families: the ryanodine receptors (RyRs; Meissner, 1994; Franzini-Armstrong and Protasi, 1997; Hamilton and Serysheva, 2009; Clarke and Hendrickson, 2016) and inositol 1,4,5-trisphosphate receptors (Patterson et al., 2004; Seo et al., 2015; Baker et al., 2017). Both types of Ca release channels are localized in the ER and in muscle in a specialized subcompartment, the SR. Other intracellular organelles that store and release Ca include mitochondria and acidic endosomal structures. One distinct feature of the RyRs is their modification by the plant alkaloid ryanodine, hence their name, which distinguishes them from other Ca channels. The RyRs are comprised of four polypeptides each of ∼5,000 amino acids and four FK506-binding proteins (FKBPs) each of ∼110 amino acids. RyRs are high-conductance, monovalentand divalent-conducting channels regulated by multiple factors that include Ca, Mg, ATP, calmodulin (CaM), protein kinases and phosphatases, and redox active species. Recent determination of the high-resolution cryo-electron microscopy (cryo-EM) structure of the skeletal muscle RyR1 and cardiac muscle RyR2 isoforms has provided insight into the complex interacting regulatory mechanisms of SR Ca release (Efremov et al., 2015; Yan et al., 2015; Zalk et al., 2015; Bai et al., 2016; des Georges et al., 2016; Peng et al., 2016; Wei et al., 2016). RyR-like structures were identified in colony-forming choanoflagellates that evolved more than 600 million years ago (Mackrill, 2012). Alterations in the activity of the RyRs have been implicated in a number of muscle diseases. More than 100 RyR1 mutations are potentially associated in skeletal muscle with malignant hyperthermia (MH), central core disease (CCD), and multi-minicore disease (MmD; Robinson et al., 2006; Treves et al., 2008), and more than 150 RyR2 mutations are potentially linked to catecholaminergic polymorphic ventricular tachycardia (CPVT), arrhythmogenic right ventricular dysplasia type 2 (ARVD2), and idiopathic ventricular fibrillation in cardiac muscle (George et al., 2007; Leenhardt et al., 2012). Here, the experimental and recent structural studies that led to our current understanding of RyR regulatory mechanisms and their structural basis are reviewed.
Quantifying the Physical Separability of RF-Based Multi-Person Respiration Monitoring via SINR

Recent years have witnessed a growing interest in contact-free respiration monitoring leveraging radio-frequency (RF) technologies. However, the proposed solutions mostly consider single-person scenarios, whereas a few multi-person monitoring proposals simply apply blind source separation to handle inter-person interference, without drawing a clear line between physical and algorithmic separability. In this paper, we set out to answer: under what condition(s) one may physically separate multiple respiration signals sensed by diversified RF technologies? Drawing inspiration from conventional signal processing, we propose respiration-to-interference-plus-noise ratio (RINR) as a novel metric, taking into account the impact from both background noise and various interfering sources. Instead of attenuation in Euclidean distance, RINR has to be evaluated upon range/angle bins where physical separation actually take place. As signal attenuation has never been modeled in this manner, we rise to this challenge by levering a deep learning model to fit a spread function upon range/angle bins. The resulting RINR model allows us to concretely indicate the limit of physical separability of RF-based multi-person respiration monitoring. Our extensive experiments firmly validate the RINR model, thus evidently demonstrating the benefits of employing RINR model as a guideline for conducting respiration monitoring with different RF technologies.
Ionic and Osmotic Mechanisms Of Insect Chill-Coma And Chilling Injury

ii! Co-authorship statement . iv! Acknowledgments . v! Table of contents . vii! List of tables . xiii! List of figures . xiv! List of videos. xvii! List of symbols and abbreviations . xviii! 1! General introduction . 1! 1.1! Biotic impacts of a changing climate . 1! 1.2! What determines thermal limits to performance? 3! 1.2. 1! There is little evidence for oxygenand capacity-limitation in terrestrial ectotherms . 4! 1.3! Insects at low temperatures . 5! 1.3. 1! The insect CTmin . 6! 1.3. 2! Freeze tolerance and freeze avoidance . 6! 1.3. 3! Chill susceptibility . 9! 1.3. 4! Variation in cold tolerance . 11! 1.3. 5! Does a loss of ion homeostasis underlie insect chill-coma and chilling injury? 11! 1.4! Insect ion and water balance . 12! 1.4. 1! Ion homeostasis in excitable tissues . 12! 1.4. 2! The insect renal system . 14! 1.5! Overview of dissertation . 16!
Genetically engineered cell membrane-coated nanoparticles for antibacterial and immunoregulatory dual-function treatment of ligature-induced periodontitis

Purpose: In order to overcome the problem that conventional pharmacological treatments of periodontitis cannot effectively synergizing antimicrobial and immunomodulation, inspired by the critical role of toll-like receptor 4 (TLR4) in bacterial recognition and immune activation, we demonstrated a combined antibacterial-immunoregulatory strategy based on biomimetic nanoparticles. Methods: Functioned cell membranes and silk fibroin nanoparticles (SNs) loaded with minocycline hydrochloride (Mino) were used to prepare a biomimetic nanoparticle (MSNCs). SNs and MSNCs were characterized by Scanning Electron Microscope, size, zeta potential, dispersion index. At the same time, SNs were characterized by cell counting kit-8 and real-time Polymerase Chain Reaction (RT-PCR). TLR4-expressing cell membranes were characterized by RT-PCR and western blot (WB). Cell membrane coating was characterized by Transmission Electron Microscope (TEM), the Bradford staining and WB. Then, Laser confocal, flow cytometry and agar plate coating were evaluated in vitro with antibacterial effects, RT-PCR was simultaneously evaluated with immunoregulatory effects. Finally, Anti-inflammatory treatment of MSNCs was evaluated in a ligature-induced periodontitis (LIP) mouse model. Results: Successfully prepared cell membranes overexpressing TLR4 and constructed MSNCs. In vitro studies had shown that MSNCs effectively targeted bacteria via TLR4 and acted as molecular decoys to competitively neutralize lipopolysaccharide (LPS) in the microenvironment as well as inhibit inflammatory activation of macrophages. In vivo, MSNCs effectively attenuated periodontal tissue inflammation and alveolar bone loss in a LIP mouse model. Conclusion: MSNCs have good targeted antibacterial and immunoregulatory effects, and provide a new and effective strategy for the treatment of periodontitis and have good potential for application in various types of pathogenic bacterial infections.
Research progress of neoantigen-based dendritic cell vaccines in pancreatic cancer

The mutation of the crucial genes such as tumor suppressors or oncogenes plays an important role in the initiation and development of tumors. The non-synonymous mutations in the tumor cell genome will produce non-autologous proteins (neoantigen) to activate the immune system by activating CD4+ and CD8+ T cells. Neoantigen-based peptide vaccines have exhibited exciting therapeutic effects in treating various cancers alone or in combination with other therapeutic strategies. Furthermore, antigen-loaded DC vaccines are more powerful in inducing stronger immune responses than vaccines generated by antigens and adjuvants. Therefore, neoantigen-based dendritic cell (DC) vaccines could achieve promising effects in combating some malignant tumors. In this review, we summarized and discussed the recent research progresses of the neoantigen, neoantigen-based vaccines, and DC-based vaccine in pancreatic cancers (PCs). The combination of the neoantigen and DC-based vaccine in PC was also highlighted. Therefore, our work will provide more detailed evidence and novel opinions to promote the development of a personalized neoantigen-based DC vaccine for PC.
Intermittent Hypoxia as an Interventional Strategy for Impaired Fasting Blood Glucose: a Systematic Review

The aim of this systematic review is to analyse the current evidence reported in the literature on the efficacy of resting in normobaric hypoxia as a potential intervention for patients with impaired fasting blood glucose. Available databases was searched, under the PRISMA guidelines, throughout to the end of August 2017. A total of four empirical studies (out of 3281 filtered) were identified as eligible for the review. Two studies were on the acute effects of one-hour exposure to moderate hypoxia, and reported a reduction of blood glucose by 0.74 to 2.1 mM. The other two studies were on the effects of repeated exposure with nine to ten sessions in two to three weeks, however did not shown sustained decrease in fasting blood glucose. These studies employed self-controlled design with relatively small numbers of participants (n= 6-18). Similar findings were also reported in studies on the effects of exercising in hypoxia with single bout or several weeks of intervention. Further research, particularly randomised controlled clinical trials, is required to determine the efficacy and hormetic dosage range of long-term IH intervention on pre-diabetes and type 2 diabetes, and to elucidate the underlying mechanisms of the treatment effects. ;
Mimicry and affective disorders

Mimicry, the spontaneous imitation of an interaction partner, is an important part of everyday communication, as it has been shown to foster relationships and increase closeness. People with affective disorders often have problems in their interpersonal lives. In this review, we pursue the question if these problems are linked to differences in mimicry behavior. First, we summarize existing evidence on mimicry, depression and mood. Then, based on five theories differing in their core assumptions regarding mechanisms and functionality of mimicry, we derive suggestions on how mimicry might affect people suffering from bipolar disorders, dysthymia or depression. Depending on each theory, a different understanding of affective disorders and mimicry arises, and we show how the evidence fit in with the suggested theories. Previous studies on affective disorders have focused on mimicry behavior of participants watching photos, computer-generated images, or short video sequences. This review sheds light on the fact that evidence on mimicry needs to be broadened systematically for people with affective disorders in interactional settings. Mimicry represents a novel and important yet underestimated source for diagnostic, intervention and evaluation processes in affective disorders.
The Association Between Low Levels of Serum Vitamin D and the Duration and Severity of Parkinson’s Disease

Background: Parkinson’s disease is a neurodegenerative disorder more commonly seen in people aged over 50 years old. The eti-ology of the disease is unknown, yet the impact of factors, such as vitamin D as a hormone on Parkinson’s disease has been demon-strated in previous studies. Objectives: The aim of the current study was to investigate the role of low levels of this factor on the severity and duration of the disease. Methods: In2015to2016, thecurrentresearchersconductedthiscase-controlstudyon50patientswithParkinson’sdiseaseand50 healthysubjectsasthecontrolgroupinBabol,Iran. Demographicinformation(age,gender,andeducation),serumlevelsof vitamin D in the two groups, disease duration, disease severity, and cardinal features of the disease in the patient group were investigated. Data were analyzed using the SPSS 23 software with the t-test, Kruskal Wallis, Mann-Whitney, and adjusted logistic regression. Results: ThemeanserumvitaminDlevelswas27.28 ± 27.75(ng/mL)and32.00 ± 17.76(ng/mL)inpatientandcontrolgroups,respec-tively (P = 0.009). In terms of the duration of the disease, 34 (68%) patients had the disease for less than ﬁve years, nine (18%) for ﬁve totenyearsandseven(14%)formorethantenyears. Thedurationwassigniﬁcantlyhigherinfemales(P=0.001). Logisticregression analysis with adjusted demographic variables showed that the observed lower levels of serum vitamin D in cases compared with the control group was statistically signiﬁcant (OR = 4.17; 95% CI: 1.37, 12.71; P = 0.012). Conclusions: TherewasasigniﬁcantrelationshipbetweenlowserumvitaminDlevelsandthedurationandseverityof Parkinson’s disease in patients under 60 years of age.
Les régimes végétariens chez les enfants et les adolescents

Résumé Un régime végétarien bien équilibré peut répondre aux besoins des enfants et des adolescents. Il faut toutefois s’assurer d’un apport calorique suffisant et prévoir une surveillance de la croissance. Il faut accorder une attention particulière à un apport suffisant de protéines et aux sources d’acides gras essentiels, de fer, de zinc, de calcium, de vitamine B12 et de vitamine D. Des suppléments peuvent être nécessaires en cas de régime strictement végétarien sans consommation de produits animaux. Les femmes enceintes et qui allaitent doivent également obtenir des conseils pour bien respecter les besoins nutritionnels du fœtus et du nourrisson. Des recommandations sont fournies. Les adolescents qui suivent un régime végétarien restreint ou d’autres régimes de ce genre devraient faire l’objet d’un dépistage de troubles alimentaires.
MSAP analysis reveals diverse epigenetic statuses in opium poppy varieties with different benzyisoquinoline alkaloid content

DNA methylation is one of the major epigenetic modifications influencing the regulation of gene expression. The opium poppy is an important medicinal plant. Its latex contains opium, which is a rich source of pharmaceutical benzyisoquinoline alkaloids (BIA). Here, the methylation-sensitive amplification polymorphism (MSAP) profiling technique using 21 MSAP molecular markers was applied in order to compare levels of DNA methylation between 6 opium poppy varieties. MSAP profiling reflected the different methylation statuses among opium poppy varieties having divergent BIA content. Moreover, different organ-specific epigenetic profiles were observed between the samples. Differential epigenetic profiles of capsules and shoots from the leaves pointed to the impact of methylation on BIA biosynthesis. The data implied that the different DNA methylation status may have important biological significance, in the case of alkaloid content in opium poppy in particular.
Prehospital Care Professional Development:does education change practice in a developing country?

Background: This study considers the impact of the Intermediate Ambulance Care (IAC) Course on the development of prehospital care practice in Penang, Malaysia and considers how the course contributes to professional development. The research question asks what impact the education program has had in a rapidly changing emergency medical system (EMS) in Penang. Methods: Using a qualitative methodology, focused ethnography, data were collected from interviews, participation and observation of pre-hospital emergency care providers and medical professionals in Penang, Malaysia. Results: Four themes were identified. The themes describe how the participants’ values and beliefs contribute to the increasing professionalization of their role in health care. Conclusion: As well as increasing paramedic knowledge there was an observable change in participants’ values and beliefs about prehospital care and health outcomes. This has contributed to the professional development currently being experienced in prehospital care in Penang, Malaysia.
The Value of the Inferior Vena Cava Area Distensibility Index and its Diameter Ratio for Predicting Fluid Responsiveness in Mechanically Ventilated Patients.

INTRODUCTION It is necessary to evaluate fluid responsiveness before fluid resuscitation. We evaluated the value of inferior vena cava (IVC) area respiratory variation and the IVC diameter ratio (IVC DR) for predicting fluid responsiveness in mechanically ventilated patients. METHODS A prospective observational study was performed in the intensive care unit between December 2017 and March 2018. Mechanically ventilated patients were enrolled and received ultrasound monitoring. IVC diameter distensibility index from the subxiphoid area (IVC-sx DDI), IVC diameter distensibility index from the right midaxillary line (IVC-rm DDI), IVC area distensibility index (IVC ADI), and IVC DR in cross-section were calculated by ultrasound monitoring IVC parameters. The enrolled patients were classified as nonresponders group and responders group according to whether the cardiac output increased by >10% after passive leg raising. RESULTS Data from 67 mechanically ventilated patients were analyzed. 55.2% of patients had positive fluid responsiveness. The area of receiver operating characteristic curves evaluating the ability of the IVC-sx DDI, IVC-rm DDI, IVC ADI, and IVC DR to predict the fluid responsiveness were 0.702, 0.686, 0.749, and 0.829, respectively. IVC DR level of 1.43 was predictive of positive fluid responsiveness with 90.0% specificity and 67.6% sensitivity. IVC ADI level of 10.2% was predictive of positive fluid responsiveness with 40.0% specificity and 97.3% sensitivity. CONCLUSIONS IVC ADI and its diameter ratio in cross-section had more value than IVC diameter distensibility index for predicting fluid responsiveness in mechanically ventilated patients.
Endovascular management of postthrombotic ilio-iliac arteriovenous fistula with occluded common iliac vein: A case report and literature review

Chronic venous hypertension as a result of May—Thurner syndrome (MTS) and subsequent venous thrombosis is a frequently observed clinical condition. However, a postthrombotic arteriovenous fistula (AVF) is a phenomenon not reported commonly. Such conditions have been treated surgically and by endovascular approach — embolization or stenting. We present a case of concomitant MTS and pelvic AVF in a patient with recurrent deep vein thrombosis, who also had lumbar laminectomy in the past, resulting in massive and debilitating left lower extremity edema and bleeding varices in the left lateral thigh, which was successfully treated with the placement of stent graft/stent in both arterial and venous systems.
Is there a difference between self-perceived performance and observed performance in an Objective Structured Clinical Examination (OSCE)? An exploratory study among medical students in the United Arab Emirates

Competency-based education and training has become a key component of healthcare systems across the globe. Ensuring that healthcare professionals are able to assess their own competencies is critical for continued professional development and the delivery of high-quality care. The aim of this study was to assess how medical students perceive their performance on an objective structured clinical examination. Using a cross-sectional study design, a sample of Emirati third and fourth year (preclinical) medical students (N=106; 56.4% response rate) was recruited from the United Arab Emirates University in Al Ain, United Arab Emirates. Medical students completed a short non-invasive clinical task (i.e. measuring and recording blood pressure and performing hand hygiene) followed by a structured survey to self-assess their performance and skills. Trained assessors used a clinical skills observation checklist tool to score each student’s performance. According to the observed performance, 27.36% of medical students performed the objective structured clinical task adequately. In contrast, 69.52% rated their own performance as adequate. Furthermore, only 8.43% environments with more eﬀective self-assessment strategies. This contributes to the understanding of the current production of Emirati medical students in the UAE, to achieve the UAE Vision 2021 and to the 2030 agenda of the Sustainable Development Goals and Universal Health Coverage.
Constraint-Induced Therapy of Chronic Aphasia After Stroke

Abstract—Patients with chronic aphasia were assigned randomly to a group to receive either conventional aphasia therapy or constraint-induced (CI) aphasia therapy, a new therapeutic technique requiring intense practice over a relatively short period of consecutive days. CI aphasia therapy is realized in a communicative therapeutic environment constraining patients to practice systematically speech acts with which they have difficulty. Patients in both groups received the same amount of treatment (30 to 35 hours) as 10 days of massed-practice language exercises for the CI aphasia therapy group (3 hours per day minimum; 10 patients) or over a longer period of ≈4 weeks for the conventional therapy group (7 patients). CI aphasia therapy led to significant and pronounced improvements on several standard clinical tests, on self-ratings, and on blinded-observer ratings of the patients’ communicative effectiveness in everyday life. Patients who received the control intervention failed to achieve comparable improv.
A systematic review and meta- analysis of randomized controlled trials to evaluate the risk of gastrointestinal and hepatic toxicities in patients with recurrent ovarian cancer treated with poly adenosine diphosphate ribose polymerase inhibitors maintenance.

Background and aim: Acute variceal bleeding (AVB) is life-threatening. We aimed to systematically review the current evidence regarding the efficacy and safety of terlipressin for AVB in liver cirrhosis. Methods:Wesearched thePubMed, EMBASE, andCochraneLibrary databases. The reference listwasalsohand-searched.Usinga random-effectmodel,wecombined thedataobtainedaccording to thedifferent timepointswhen theeventsdeveloped.Odds ratio (OR) and weighted mean difference (WMD) were calculated. Quality of evidence was evaluated by the GRADE methodology. Results: Thirty randomized controlled trials with 3344 patients were included. Compared with no vasoactive drug, terlipressin significantly improved the control of bleeding within 48hours (OR=2.94, P= .0008) and decreased the in-hospital mortality (OR= 0.31, P= .008). Compared with somatostatin, terlipressin had a significantly higher risk of complications (OR=2.44, P= .04). Compared with octreotide, terlipressin had a significantly inferior control of bleeding within 24hours (OR=0.37, P= .007). Compared with vasopressin, terlipressin had a significantly lower risk of complications (OR=0.15, P= .02). Compared with terlipressin combined with endoscopic variceal ligation, terlipressin alone had significantly higher 5-day treatment failure (OR=14.46, P= .01) and transfusion requirements within 49 to 120hours (WMD=1.20, P= .002). No outcome was significantly different between terlipressin and sclerotherapy. Compared with balloon tamponade, terlipressin significantly decreased the 30-day rebleeding (OR= 0.05, P= .001) and transfusion requirements (WMD= 2.70, P= .02). Quality of evidence was very low to moderate. Conclusion:Our findings were in accordance with the current recommendations regarding terlipressin for the treatment of AVB in cirrhosis. However, due to low quality of evidence, further studies are recommended. Abbreviations: AVB= acute variceal bleeding, CI= confidence interval, EVL= endoscopic variceal ligation, OR= odds ratio, RCT = randomized controlled trial, WMD = weighted mean difference.