Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Age on Bone Structure Parameters in Laying Hens

Simple Summary Laying hens supply calcium to their eggshells via a process of dynamic bone formation and resorption called remodeling. As hens age, their bone becomes weak and brittle due to an imbalance in remodeling, resulting in bone disorders. Osteoporosis, characterized by bone mass loss and increased risk of fractures, is a serious problem in the egg industry, affecting both productivity and animal welfare. Nonetheless, the mechanisms of age-related osteoporosis remain unknown. We evaluated differences in bone structure between age groups, using microscopy and 3D imaging. Bone quality was reduced by an increase in porosity, with loss of volume, contributing to age-related osteoporosis. Abstract Changes in medullary and cortical bone structure with age remain unclear. Twenty Hy-Line W36 hens, 25 or 52 weeks of age, were euthanized, and both tibiae were collected when an egg was present in the magnum. Serial cross sections of the tibiae were stained with Alcian blue. The bones were scanned using micro-computed tomography. Trabecular width (Tb.Wi) was significantly higher (p < 0.05) in 25-week-old hens, whereas medullary bone tissue volume (TV) was significantly higher (p < 0.01) in 52-week-old hens. 25-week-old hens had significantly higher (p < 0.01) bone volume fraction (BVF = calcified tissue / TV). Moreover, the cortical bone parameters were significantly higher (TV and bone mineral content (BMC) at p < 0.05, and bone volume (BV) and BVF at p < 0.01) in younger hens. Open porosity and total porosity, which indicate less density, were significantly higher (p < 0.01) in older hens. Older hens showed significantly higher (p < 0.01) tibial diaphysis TV than younger hens. Younger hens had significantly higher (p < 0.01) BV, BVF and bone mineral density (BMD) of the tibial diaphysis. These findings reveal that reductions in medullary bone quality might be associated with age-related low estrogen levels and stimulation of osteoclastic bone resorption by parathyroid hormone. Cortical bone quality decreased with enlargement of the Haversian canals and loss of volume, with a longer egg-laying period leading to osteoporosis.
Native Vineyard Non-Saccharomyces Yeasts Used for Biological Control of Botrytis cinerea in Stored Table Grape

Postharvest spoilage fungi, such as Botrytis cinerea, are considered the main cause of losses of fresh fruit quality and vegetables during storage, distribution, and consumption. The current control strategy is the use of SO2 generator pads whose application is now largely under observation. A high quantity of SO2 can be deleterious for fresh fruits and vegetables and it is not allowed in organic agriculture. For this reason, great attention has been recently focused on identifying Biological Control Agents (BCA) to implement biological approaches devoid of chemicals. In this direction, we carried out our study in isolating five different non-Saccharomyces yeast strains from local vineyards in the South of Italy as possible BCA. We performed both in vitro and in vivo assays in semi-commercial conditions on detached grape berries stored at 0 °C, simulating the temperature normally used during cold storage, and obtained relevant results. We isolated three M. pulcherrima strains and one L. thermotolerans strain able to largely antagonize the development of the B. cinerea, at both in vitro and in vivo conditions. In particular, we detected the ability of the three isolates of M. pulcherrima strains Ale4, N20/006, and Pr7 and the L. thermotolerans strain N10 to completely inhibit (100% in reduction) the mycelial growth of B. cinerea by producing fungistatic compounds. We found, using an extracellular lytic enzymes activity assay, that such activity could be related to lipid hydrolyzation, β-1,3-glucanase and pectinase activity, and pectinase and protease activity, depending on the yeasts used. Results from our in vitro assays allowed us to hypothesize for M. pulcherrima strains Ale4 and N20/006 a possible combination of both the production of soluble metabolites and volatile organic compounds to antagonize against B. cinerea growth. Moreover, in semi-commercial conditions, the M. pulcherrima strain N20/006 and L. thermotolerans strain N10 showed relevant antagonistic effect also at low concentrations (with a significantly reduction of ‘slip skin’ incidence of 86.4% and 72.7%, respectively), thus highlighting a peculiar property to use in commercial development for organic agriculture and the handling process.
Differential Interactome Proposes Subtype-Specific Biomarkers and Potential Therapeutics in Renal Cell Carcinomas

Although many studies have been conducted on single gene therapies in cancer patients, the reality is that tumor arises from different coordinating protein groups. Unveiling perturbations in protein interactome related to the tumor formation may contribute to the development of effective diagnosis, treatment strategies, and prognosis. In this study, considering the clinical and transcriptome data of three Renal Cell Carcinoma (RCC) subtypes (ccRCC, pRCC, and chRCC) retrieved from The Cancer Genome Atlas (TCGA) and the human protein interactome, the differential protein–protein interactions were identified in each RCC subtype. The approach enabled the identification of differentially interacting proteins (DIPs) indicating prominent changes in their interaction patterns during tumor formation. Further, diagnostic and prognostic performances were generated by taking into account DIP clusters which are specific to the relevant subtypes. Furthermore, considering the mesenchymal epithelial transition (MET) receptor tyrosine kinase (PDB ID: 3DKF) as a potential drug target specific to pRCC, twenty-one lead compounds were identified through virtual screening of ZINC molecules. In this study, we presented remarkable findings in terms of early diagnosis, prognosis, and effective treatment strategies, that deserve further experimental and clinical efforts.
The Pentatricopeptide Repeat Protein MEF100 Is Required for the Editing of Four Mitochondrial Editing Sites in Arabidopsis

In Arabidopsis thaliana there are more than 600 C-to-U RNA editing events in the mitochondria and at least 44 in the chloroplasts. Pentatricopeptide repeat (PPR) proteins provide the specificity for these reactions. They recognize RNA sequences in a partially predictable fashion via key amino acids at the fifth and last position in each PPR motif that bind to individual ribonucleotides. A combined approach of RNA-Seq, mutant complementation, electrophoresis of mitochondrial protein complexes and Western blotting allowed us to show that MEF100, a PPR protein identified in a genetic screen for mutants resistant to an inhibitor of γ -glutamylcysteine synthetase, is required for the editing of nad1-493, nad4-403, nad7-698 and ccmFN2-356 sites in Arabidopsis mitochondria. The absence of editing in mef100 leads to a decrease in mitochondrial Complex I activity, which probably explains the physiological phenotype. Some plants have lost the requirement for MEF100 at one or more of these sites through mutations in the mitochondrial genome. We show that loss of the requirement for MEF100 editing leads to divergence in the MEF100 binding site.
Detecting Single Molecules in Liquids

An important challenge facing analytical chemists today is the efficient detection and identification of single molecules. Over the past few years, several fluorescence-based techniques have evolved with sufficient sensitivity to detect single molecules. There are advantages and disadvantages associated with each technique. The principal trade-off is sample throughput versus S/N. The technique of choice for a particular application would of course depend on the priorities of the experiment. In this Report, we discuss the nature of this trade-off by considering the intrinsic sensitivity limitations involved in single-molecule fluorescence detection in liquids and give some examples of different detection schemes. 35 refs., 4 figs.
Effects of polyamine structure on rosin sizing under neutral papermaking conditions

The sizing effectiveness of rosin size with four typical polyamines, polyvinylamine (PVAm), polyallylamine (PAAm), poly(dimethylamino ethyl methacrylate) (PDMAEMA), and polyethyleneimine (PEI), was investigated under neutral papermaking conditions. The polyamines with linear structures were more effective than was PEI with a branched structure. The smaller the side chains of the linear polyamine, the larger was the sizing degree of the rosin emulsion size (RE) with the polyamine. The polyamine with smaller molecular geometric size could retain more rosin size on the pulp and form smaller rosin-polyamine complexes.
Examination of Staphylococcus aureus Prophages Circulating in Egypt

Staphylococcus aureus infections are of growing concern given the increased incidence of antibiotic resistant strains. Egypt, like several other countries, has seen alarming increases in methicillin-resistant S. aureus (MRSA) infections. This species can rapidly acquire genes associated with resistance, as well as virulence factors, through mobile genetic elements, including phages. Recently, we sequenced 56 S. aureus genomes from Alexandria Main University Hospital in Alexandria, Egypt, complementing 17 S. aureus genomes publicly available from other sites in Egypt. In the current study, we found that the majority (73.6%) of these strains contain intact prophages, including Biseptimaviruses, Phietaviruses, and Triaviruses. Further investigation of these prophages revealed evidence of horizontal exchange of the integrase for two of the prophages. These Egyptian S. aureus prophages are predicted to encode numerous virulence factors, including genes associated with immune evasion and toxins, including the Panton–Valentine leukocidin (PVL)-associated genes lukF-PV/lukS-PV. Thus, prophages are likely to be a major contributor to the virulence of S. aureus strains in circulation in Egypt.
Development of Antimicrobial Phototreatment Tolerance: Why the Methodology Matters

Due to rapidly growing antimicrobial resistance, there is an urgent need to develop alternative, non-antibiotic strategies. Recently, numerous light-based approaches, demonstrating killing efficacy regardless of microbial drug resistance, have gained wide attention and are considered some of the most promising antimicrobial modalities. These light-based therapies include five treatments for which high bactericidal activity was demonstrated using numerous in vitro and in vivo studies: antimicrobial blue light (aBL), antimicrobial photodynamic inactivation (aPDI), pulsed light (PL), cold atmospheric plasma (CAP), and ultraviolet (UV) light. Based on their multitarget activity leading to deleterious effects to numerous cell structures—i.e., cell envelopes, proteins, lipids, and genetic material—light-based treatments are considered to have a low risk for the development of tolerance and/or resistance. Nevertheless, the most recent studies indicate that repetitive sublethal phototreatment may provoke tolerance development, but there is no standard methodology for the proper evaluation of this phenomenon. The statement concerning the lack of development of resistance to these modalities seem to be justified; however, the most significant motivation for this review paper was to critically discuss existing dogma concerning the lack of tolerance development, indicating that its assessment is more complex and requires better terminology and methodology.
Sensors and Measurements for Unmanned Systems: An Overview

The advance of technology has enabled the development of unmanned systems/vehicles used in the air, on the ground or on/in the water. The application range for these systems is continuously increasing, and unmanned platforms continue to be the subject of numerous studies and research contributions. This paper deals with the role of sensors and measurements in ensuring that unmanned systems work properly, meet the requirements of the target application, provide and increase their navigation capabilities, and suitably monitor and gain information on several physical quantities in the environment around them. Unmanned system types and the critical environmental factors affecting their performance are discussed. The measurements that these kinds of vehicles can carry out are presented and discussed, while also describing the most frequently used on-board sensor technologies, as well as their advantages and limitations. The paper provides some examples of sensor specifications related to some current applications, as well as describing the recent research contributions in the field.
Inhibition of the Sodium Calcium Exchanger Suppresses Alcohol Withdrawal-Induced Seizure Susceptibility

Calcium influx plays important roles in the pathophysiology of seizures, including acoustically evoked alcohol withdrawal-induced seizures (AWSs). One Ca2+ influx route of interest is the Na+/Ca2+ exchanger (NCX) that, when operating in its reverse mode (NCXrev) activity, can facilitate Ca2+ entry into neurons, possibly increasing neuronal excitability that leads to enhanced seizure susceptibility. Here, we probed the involvement of NCXrev activity on AWS susceptibility by quantifying the effects of SN-6 and KB-R7943, potent blockers of isoform type 1 (NCX1rev) and 3 (NCX3rev), respectively. Male, adult Sprague–Dawley rats were used. Acoustically evoked AWSs consisted of wild running seizures (WRSs) that evolved into generalized tonic–clonic seizures (GTCSs). Quantification shows that acute SN-6 treatment at a relatively low dose suppressed the occurrence of the GTCSs (but not WRSs) component of AWSs and markedly reduced the seizure severity. However, administration of KB-R7943 at a relatively high dose only reduced the incidence of GTCSs. These findings demonstrate that inhibition of NCX1rev activity is a putative mechanism for the suppression of alcohol withdrawal-induced GTCSs.
Loading Recommendations for Muscle Strength, Hypertrophy, and Local Endurance: A Re-Examination of the Repetition Continuum

Loading recommendations for resistance training are typically prescribed along what has come to be known as the “repetition continuum”, which proposes that the number of repetitions performed at a given magnitude of load will result in specific adaptations. Specifically, the theory postulates that heavy load training optimizes increases maximal strength, moderate load training optimizes increases muscle hypertrophy, and low-load training optimizes increases local muscular endurance. However, despite the widespread acceptance of this theory, current research fails to support some of its underlying presumptions. Based on the emerging evidence, we propose a new paradigm whereby muscular adaptations can be obtained, and in some cases optimized, across a wide spectrum of loading zones. The nuances and implications of this paradigm are discussed herein.
Burnout and Cognitive Performance

The aim of this study was to investigate the relationship between burnout and cognitive functioning. The associations of depression, anxiety and family support with burnout and cognitive functioning were also examined both independently and as potential moderators of the burnout–cognitive functioning relationship. Seven different cognitive tasks were administered to employees of the general working population and five cognitive domains were assessed; i.e., executive functions, working memory, memory (episodic, visuospatial, prospective), attention/speed of processing and visuospatial abilities. Burnout, depression, anxiety and family support were assessed with the Maslach Burnout Inventory-General Survey, the Hospital Anxiety and Depression Scale and the Family Support Scale respectively. In congruence with the first and fourth (partially) Hypotheses, burnout and perceived family support are significantly associated with some aspects of cognitive functioning. Moreover, in line with the third Hypothesis, perceived family support is inversely related to burnout. However, in contrast to the second and fourth Hypotheses, depression, anxiety and perceived family support do not moderate the burnout–cognitive functioning relationship. Additional results reveal positive associations between burnout depression and anxiety. Overall findings suggest that cognitive deficits, depression and anxiety appear to be common in burnout while they underpin the role of perceived family support in both mental health and cognitive functioning. Implications for practice are discussed.
Noise Annoyance in the UAE: A Twitter Case Study via a Data-Mining Approach

Noise pollution is a growing global public health concern. Among other issues, it has been linked with sleep disturbance, hearing functionality, increased blood pressure and heart disease. Individuals are increasingly using social media to express complaints and concerns about problematic noise sources. This behavior—using social media to post noise-related concerns—might help us better identify troublesome noise pollution hotspots, thereby enabling us to take corrective action. The present work is a concept case study exploring the use of social media data as a means of identifying and monitoring noise annoyance across the United Arab Emirates (UAE). We explored an extract of Twitter data for the UAE, comprising over eight million messages (tweets) sent during 2015. We employed a search algorithm to identify tweets concerned with noise annoyance and, where possible, we also extracted the exact location via Global Positioning System (GPS) coordinates) associated with specific messages/complaints. The identified noise complaints were organized in a digital database and analyzed according to three criteria: first, the main types of the noise source (music, human factors, transport infrastructures); second, exterior or interior noise source and finally, date and time of the report, with the location of the Twitter user. This study supports the idea that lexicon-based analyses of large social media datasets may prove to be a useful adjunct or as a complement to existing noise pollution identification and surveillance strategies.
Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

Owing to the prohibition of cosmetic animal testing, various attempts have recently been made using skin-on-a-chip (SOC) technology as a replacement for animal testing. Previously, we reported the development of a pumpless SOC capable of drug testing with a simple drive using the principle that the medium flows along the channel by gravity when the chip is tilted using a microfluidic channel. In this study, using pumpless SOC, instead of drug testing at the single-cell level, we evaluated the efficacy of α-lipoic acid (ALA), which is known as an anti-aging substance in skin equivalents, for skin tissue and epidermal structure formation. The expression of proteins and changes in genotyping were compared and evaluated. Hematoxylin and eosin staining for histological analysis showed a difference in the activity of fibroblasts in the dermis layer with respect to the presence or absence of ALA. We observed that the epidermis layer became increasingly prominent as the culture period was extended by treatment with 10 μM ALA. The expression of epidermal structural proteins of filaggrin, involucrin, keratin 10, and collagen IV increased because of the effect of ALA. Changes in the epidermis layer were noticeable after the ALA treatment. As a result of aging, damage to the skin-barrier function and structural integrity is reduced, indicating that ALA has an anti-aging effect. We performed a gene analysis of filaggrin, involucrin, keratin 10, integrin, and collagen I genes in ALA-treated human skin equivalents, which indicated an increase in filaggrin gene expression after ALA treatment. These results indicate that pumpless SOC can be used as an in vitro skin model similar to human skin, protein and gene expression can be analyzed, and it can be used for functional drug tests of cosmetic materials in the future. This technology is expected to contribute to the development of skin disease models.
Fluorous synthesis with fewer fluorines (light fluorous synthesis): Separation of tagged from untagged products by solid-phase extraction with fluorous reverse-phase silica gel

Fluorous synthesis involves tagging an organic substrate with a fluorinated tag for the purposes of separation. To date, techniques of fluorous synthesis have relied on liquid−liquid extractions. This paper applies a simple solid−liquid extraction procedure over fluorous reverse-phase silica gel (silica with a fluorocarbon bonded phase) for use in fluorous synthesis. Four amino acids were tagged on nitrogen with the C9F19CO− group, and the resulting acids were coupled in a parallel experiment with an excess of four amines. The resulting 16 crude fluorous amide products were separated from all the coupling reagents and excess amine by two-stage filtration through fluorous silica. In 15 of the 16 cases, the products were isolated in good to excellent yield and purity. All of the products are soluble in organic solvents and none is expected to have any significant solubility in fluorous solvents, so the experiment dramatically illustrates the advantages of the solid−liquid extraction over the liquid−liquid e.
Stability of plastics monomers in food-simulating liquids under European Union migration test conditions

The stability of 24 plastics monomers permitted for use in the European Union (EU) for food contact applications was studied in food-simulating liquid under various heating regimes. The monomers were heated in each of the food simulants (distilled water, 15% ethanol, 3% acetic acid, and olive oil) under conditions stipulated in EU regulations for testing for compliance with migration limits. Of the 24 monomers studied, 21 showed a loss in one or more of the simulants: for 14 substances the loss was 50% or greater, and 6 of the monomers were completely lost under at least one of the exposure conditions tested
Infrared identification of carboxylic acids formed in polymer photooxidation

Several low molecular weight carboxylic acids with different substitutions were introduced into various polymer matrices: polypropylene, polystyrene, poly(butylene terephthalate), MDI-based polyurethane, and poly(vinyl chloride). These acids were then submitted to chemical treatments by sulfur tetrafluoride and ammonia. Derivatization reactions by SF4 and NH3 gave, respectively, acyl fluorides and carboxylate ions. The wavenumbers corresponding to the vco maxima of the carboxylic acids, acyl fluorides, and carboxylate ions were determined. For each one of these species, noticeable shifts were recorded that depended on the nature of the substitutents as well as on the nature of the polymer matrix. In addition, samples of the chosen polymers were exposed to photoaging. Photooxidized samples were reacted with SF4 and NH3. Their IR characteristics were determined before and after the treatments. Comparison with the results obtained with the molecular acids permitted us to specify the chemical structure of the acids formed by photooxidation and then to determine the oxidation sites on the macromolecular chains of the different polymers. © 1994 John Wiley & Sons, Inc.