Miracle-dz/UniFAR-FTUs huggingface dataset:
Comprehensive Analysis of the Glycolysis-Related Gene Prognostic Signature and Immune Infiltration in Endometrial Cancer

Glucose metabolic reprogramming and immune imbalance play important roles in the progression of cancers. The purpose of this study is to develop a glycolysis-related prognostic signature for endometrial cancer (EC) and analyze its relationship with immune function. The mRNA expression profiling of the glycolysis-related genes and clinical data of EC patients were downloaded from The Cancer Genome Atlas (TCGA). We identified a glycolysis-related gene prognostic signature for predicting the prognosis of EC by using The Least Absolute Shrinkage and Selection Operator (LASSO) regression and found the patients in the high-risk group had worse survival prognosis. Multivariate Cox regression analysis showed that the gene signature was an independent prognostic factor for EC. The ROC curve confirmed the accuracy of the prognostic signature (AUC = 0.730). Then, we constructed a nomogram to predict the 1–5 years survival rate of EC patients. The association between the gene signature and immune function was analyzed based on the “ESTIMATE” and “CIBERSORT” algorithm, which showed the immune and ESTIMATE scores of patients in the high-risk group were lower, while the low immune and ESTIMATE scores were associated with a worse prognosis of patients. The imbalance of immune cells was also found in the high-risk group. Further, the protein of CDK1, a gene in the signature, was found to be closely related to prognosis of EC and inhibition of CDK1 could inhibit migration and promote apoptosis of EC cells. This study reveals a link between glycolysis-related gene signature and immunity, and provides personalized therapeutic targets for EC.
Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)

Longan (Dimocarpus longan Lour.) is an important subtropical fruit tree in China. Nearly 90% of longan fruit imports from Thailand are from the cultivar Yiduo. However, we have observed that there exists a unilateral cross incompatibility (UCI) when Yiduo is used as a female parent and Shixia (a famous Chinese cultivar) as a male parent. Here, we performed a comparative transcriptome analysis coupled with microscopy of pistils from two reciprocal pollination combinations [Shixia♂ × Yiduo♀(SY) and Yiduo♀ × Shixia♂(YS)] 4, 8, 12, and 24 h after pollination. We also explored endogenous jasmonic acid (JA) and jasmonyl isoleucine (JA-Ile) levels in pistils of the crosses. The microscopic observations showed that the UCI was sporophytic. The endogenous JA and JA-Ile levels were higher in YS than in SY at the studied time points. We found 7,251 differentially expressed genes from the transcriptome analysis. Our results highlighted that genes associated with JA biosynthesis and signaling, pollen tube growth, cell wall modification, starch and sucrose biosynthesis, and protein processing in endoplasmic reticulum pathways were differentially regulated between SY and YS. We discussed transcriptomic changes in the above-mentioned pathways regarding the observed microscopic and/or endogenous hormone levels. This is the first report on the elaboration of transcriptomic changes in longan reciprocal pollination combination showing UCI. The results presented here will enable the longan breeding community to better understand the mechanisms of UCI.
A Comprehensive Monitoring and Assessment System for Multiple Fisheries Resources in the Northwest Pacific Based on Satellite Remote Sensing Technology

Monitoring the dynamics of ocean-going fishing vessels is essential for fisheries stock assessment and management. In this paper, coupled fishery and remote sensing methods were applied to propose a satellite-based fishery resource monitoring and assessment system (SFRMAS) that supported chub mackerel (Scomber japonicus), Pacific saury (Cololabis saira) and neon flying squid (Ommastrephes bartramii) fishing activities in the northwest Pacific. A multispecies fishing vessel database was generated in the SFRMAS using visible infrared imaging radiometer suite day/night band (VIIRS/DNB) images, automatic identification system (AIS) data and multimarine remote sensing resources. The results showed that the VIIRS/DNB vessel detection (VVD) and AIS vessel detection approaches improved the extraction accuracy of multispecies fishing vessels. A high productivity area with a higher chlorophyll a concentration had the most concentrated distribution of mackerel vessels. The widest longitudinal migrations of the neon flying squid and the latitudinal ranges of Pacific saury were highly related to sea surface temperature (SST), which was well captured by the movement of the fishing vessels. In the SFRMAS, the accuracy of detected multispecies fishing days and fishing catches was mostly between 60 and 90%, which can provide data support for fisheries stock assessment and management.
Characterization of Geochemistry in Hydrothermal Sediments From the Newly Discovered Onnuri Vent Field in the Middle Region of the Central Indian Ridge

The recently discovered Onnuri hydrothermal vent field (OVF) is a typical off-axis ultramafic-hosted vent system, located on the summit of the dome-like ocean core complex (OCC) at a distance of ∼12 km from the ridge axis along the middle region of the Central Indian Ridge (CIR). The plume chemistry with high methane anomaly was consistent with the precursor of hydrothermal activity; however, the fundamental characteristic of the OVF system, such as the hydrothermal circulation process and source of heat, remains poorly understood. Here, we focus on the geochemical features of surface sediments and minerals collected at and around the OVF region in order to better understand this venting system. The results reveal that the OVF sediments are typified by remarkably high concentrations of Fe, Si, Ba, Cu, and Zn, derived from hydrothermal fluid and S and Mg from seawater; depleted C-S isotope compositions; and abundant hydrothermally precipitated minerals (i.e., Fe–Mn hydroxides, sulfide and sulfate minerals, and opal silica). Notably, the occurrence of pure talc and barite bears witness to strong hydrothermal activity in the OVF, and their sulfur and strontium isotope geochemistry agree with extensive mixing of the unmodified seawater with high-temperature fluid derived from the gabbroic rock within the ultramafic-dominated ridge segment. The findings reveal that the OVF is a representative example of an off-axis, high-temperature hydrothermal circulation system, possibly driven by the exothermic serpentinization of exposed peridotites. Given the widespread distribution of OCC with detachment faults, furthermore, the OVF may be the most common type of hydrothermal activity in the CIR, although the paucity of data precludes generalizing this result. This study provides important information contributing to our understanding of the ultramafic-hosted hydrothermal vent system with a non-magmatic heat source along mid-ocean ridges.
Motor Unit Number Estimation (MUNE) Free of Electrical Stimulation or M Wave Recording: Feasibility and Challenges

The motor unit is the basic organizational and functional element for neuromuscular control. In motor neuron diseases, such as amyotrophic lateral sclerosis and spinal muscular atrophy, motor neurons progressively degenerate, resulting in a reduced number of motor units. Loss of motor units is also a major factor in development of sarcopenia (Gilmore et al., 2017). Although motor unit loss can be partially compensated by axonal branching and muscle fiber reinnervation, such compensation becomes insufficient with disease progression. As a result, patients suffer from progressive muscle weakness and loss of function. The number of motor units provides an important biomarker for diagnosing neuromuscular disease, tracking disease progression, and evaluating the effect of treatments and therapies (Olney and Lomen-Hoerth, 2000; Cudkowicz et al., 2006; Neuwirth et al., 2015). This has led to significant efforts in the past 50 years towardmotor unit number estimation (MUNE).
Development and Validation of a Novel Model to Predict Regional Lymph Node Metastasis in Patients With Hepatocellular Carcinoma

Background The evaluation of the nodal status of hepatocellular carcinoma (HCC) is a classic but controversial topic. This study aimed to investigate the incidence of lymph node metastasis (LNM), explore the role of lymph node dissection (LND), and develop and validate a novel model to predict LNM in patients with HCC, not other specified (NOS). Methods The study cohort was taken from the Surveillance, Epidemiology, and End Results database. The annual percent change (APC) was calculated using the Joinpoint regression. Survival analyses adopted the competing risk model. The nomogram was constructed based on the least absolute shrinkage and selection operator (LASSO) logistic regression algorithm and validated by calibration curves. The area under the receiver operating characteristic curve (AUROC) was obtained to compare prognostic performance. Decision curve and clinical impact curve analyses were introduced to examine the clinical value of the models. Results A total of 8,829 patients were finally enrolled in this study, and 1,346 (15.2%) patients received LND. The LND rate showed no noticeable fluctuation in the last decade, with an APC of 0.5% (P=0.593). LNM was identified in 56 (4.2%) patients and confirmed an independent prognostic factor of HCC patients (P=0.005). There were 2,497 lymph nodes retrieved, and 93 (3.7%) of them were positive. After propensity score matching, LND indicated no direct oncologic benefit and did not worsen competing risks. Moreover, an increased number of lymph nodes retrieved could not improve prognoses. 1,346 patients with LND were further randomly divided into the training and validation sets with the ratio of 1:1. Race, tumor size, clinical T stage, extrahepatic bile duct invasion, and tumor grade were independent risk factors for LNM. The constructed model was well calibrated and showed good discrimination power and net benefits in clinical practice. Conclusion LNM is an independent prognostic factor in HCC, but routine LND seems to be unnecessary in HCC patients. The constructed model could predict the presence of LNM in HCC patients with good performance, which is meaningful to patient stratification and individual treatment strategies optimization.
Quality of Life After Vestibular Schwannoma Surgery: A Question of Perspective

Objective Health-related quality of life (HRQoL) and self-reported outcome measures have a relevant impact on the medical decision-making process. They capture either the current status and allow for multiple prospective evaluations in the course of a treatment or rely on the retrospective comparison of health of patients before and after an intervention to assess its benefit. Importantly, these patient-assessed measures may be influenced by psychological factors. We compared HRQoL and perceived benefit in the course of surgical vestibular schwannoma (VS) treatment, as assessed by the patients from a prospective and retrospective point-of-view, and evaluated the influence of co-morbid depression. Methods Within a prospective observational single-center study, forty-three patients with VS were investigated before and after retrosigmoid tumor resection. SF-36, Beck Depression Inventory and patient-assessed clinical symptoms were acquired before surgery and at follow-up. At follow-up, the Glasgow Benefit Inventory (GBI) was acquired as well. Results SF-36 scores were significantly lower than the age and sex matched normative data in six and three out of eight categories before and after surgery, respectively. Three categories improved significantly after vs. before surgery; one of them (global health) reached a minimal clinical important difference. In contrast, patients reported predominantly a deterioration, when asked for a retrospective evaluation of the benefit (i.e., GBI). Depression correlated with both SF-36 and GBI, determined dissatisfaction, improved significantly after surgery and was the measure that had the largest impact on HRQoL. Conclusion Prospective and retrospective HRQoL measures may lead to different findings and can be confounded by psychological factors.
Calculation and Analysis on Power Frequency Magnetic Field Induced Current Density within Human Body under UHVAC Transmission Line

At present, influence of AC magnetic field on human body and zoological environment becomes a focus problem concerned by human beings gradually. The alternating electromagnetic field of UHV transmission line will induce current within human body while human body is under Ultra-High Voltage (UHV) transmission line; when the density of induced current exceeds a certain limitation, it will affect human health caused by the stimulation or damage of nerve cell tissue because the energy of electro-magnetic field absorbed by human body turns into heat energy and leads to fever of human body. By means of finite element analysis in three-dimension vortex field, the inductive current within human body induced by power frequency alternating magnetic field under 1000kV transmission line is calculated; the distribution feature of induced current distributed within human body as well as the principal factors impacting the value of induced current within human body are analyzed. Research results show that under the power frequency magnetic field of 1000kV transmission line, the density of induced current within human body is far less than the safety limit of body current density 10mA/m2. Thus, the conclusion obtained from this research is that the controlled condition of least height to earth for 1000kV transmission line does not depend on the induced value of magnetic field.
A Prediction Model for Acute Kidney Injury After Pericardiectomy: An Observational Study

Objectives Acute kidney injury is a common complication after pericardiectomy for constrictive pericarditis, which predisposes patients to worse outcomes and high medical costs. We aimed to investigate potential risk factors and consequences and establish a prediction model. Methods We selected patients with constrictive pericarditis receiving isolated pericardiectomy from January 2013 to January 2021. Patients receiving concomittant surgery or repeat percardiectomy, as well as end-stage of renal disease were excluded. Acute kidney injury was diagnosed and classified according to the KDIGO criteria. Clinical features were compared between patients with and without postoperative acute kidney injury. A prediction model was established based on multivariable regression analysis. Results Among two hundred and eleven patients, ninety-five (45.0%) developed postoperative acute kidney injury, with fourty-three (45.3%), twenty-eight (29.5%), and twenty-four (25.3%) in mild, moderate and severe stages, respectively. Twenty-nine (13.7%) patients received hemofiltration. Nine (4.3%) patients died perioperatively and were all in the acute kidney injury (9.5%) group. Eleven (5.2%) patients were considered to have chronic renal dysfunction states at the 6-month postoperative follow-up, and eight (72.7%) of them experienced moderate to severe stages of postoperative acute kidney injury. Univariable analysis showed that patients with acute kidney injury were older (difference 8 years, P < 0.001); had higher body mass index (difference 1.68 kg·m−2, P = 0.002); rates of smoking (OR = 2, P = 0.020), hypertension (OR = 2.83, P = 0.004), and renal dysfunction (OR = 3.58, P = 0.002); higher central venous pressure (difference 3 cm H2O, P < 0.001); and lower cardiac index (difference −0.23 L·min−1·m−2, P < 0.001) than patients without acute kidney injury. Multivariable regression analysis showed that advanced age (OR 1.03, P = 0.003), high body mass index (OR 1.10, P = 0.024), preoperative atrial arrhythmia (OR 3.12, P = 0.041), renal dysfunction (OR 2.70 P = 0.043), high central venous pressure (OR 1.12, P = 0.002), and low cardiac index (OR 0.36, P = 0.009) were associated with a high risk of postoperative acute kidney injury. A nomogram was established based on the regression results. The model showed good model fitness (Hosmer-Lemeshow test P = 0.881), with an area under the curve value of 0.78 (95% CI: 0.71, 0.84, P < 0.001). Conclusion The prediction model may help with early recognition, management, and reduction of acute kidney injury after pericardiectomy.
Impact of Different Synchrotron Flattop Operation Modes on 4D Dosimetric Uncertainties for Scanned Carbon-Ion Beam Delivery

Purpose The characteristic of pulsed beam delivery for synchrotron-based carbon-ion radiotherapy has led to the emergence of many scanning scenarios in order to improve the treatment efficiency and accuracy of moving target volume. Here, we aim to evaluate a novel breathing guidance motion mitigation performance under different synchrotron flattop operation modes in carbon-ion radiotherapy. Methods With the use of twelve 4DCT datasets of lung cancer patients who had been treated with respiratory-gated carbon-ion pencil beam therapy, range-adapted internal target volume (raITV) plans were optimized. Under the fixed flattop with single-energy and extended flattop with multi-energy synchrotron operation modes, the 4D treatments with breathing guidance and free breathing-based gated phase-controlled rescanning (PCR) beam delivery were simulated. Dose metrics (D95 and D5–D95 in clinical target volume (CTV)) and treatment time of the resulting 4D plans were compared. Results The two synchrotron operation modes provided different scanning dynamics. For the free breathing-based PCR method delivered in the extended flattop operation mode, the averaged CTV-D95 values were 90.4% ± 3.7%, 95.4% ± 1.7%, 96.9% ± 1.5%, 97.2% ± 1.5%, and 97.3% ± 1.5% for the 1-scanning, 2-PCR, 4-PCR, 6-PCR, and 8-PCR, respectively. For the breathing guidance-based PCR method delivered in the extended flattop mode, these values were 89.1% ± 4.0%, 97.0% ± 1.4%, 98.2% ± 0.7%, 98.6% ± 0.7%, and 98.9% ± 0.7%, respectively. However, CTV-D95 significantly increased to 98.5% ± 1.0% even with just 1-scanning breathing guidance-based fixed flattop operation mode (p < 0.01). Moreover, there was no significant difference in treatment time among the three technical combinations (p > 0.15). Conclusions The combination of the breathing guidance and PCR methods should be an alternative way for motion mitigation for the fixed flattop synchrotron operation mode. The target dose coverage and homogeneity could be further improved by the combination of the breathing guidance and PCR methods than the traditional PCR-only technology for the extended flattop synchrotron operation mode.
Association of the Serum Folate and Total Calcium and Magnesium Levels Before Ovarian Stimulation With Outcomes of Fresh In Vitro Fertilization Cycles in Normogonadotropic Women

Background Women of reproductive age are recommended to consume folic acid and other supplements before conception and during pregnancy. We aimed to investigate the association of the serum folate and total magnesium (Mg) and calcium (Ca) levels before ovarian stimulation with the outcomes of assisted reproductive technology (ART) in normogonadotropic women. Methods We used a subanalysis of data obtained from a multicentre, randomized prospective study (NCT03088137). A total of 110 normogonadotropic, non-advanced aged, non-obese women with tubal and/or male infertility factors were enrolled for the single fresh ovarian stimulation GnRH antagonist cycle. The main outcome measures were the total oocyte yield, mature oocytes, fertilization rate, biochemical, clinical pregnancy, and live birth. Multivariable generalized linear models adjusted for covariates were used with a Poisson distribution and the log link function for adjusted oocyte counts, and a binomial distribution and the log link function were used for adjusted clinical ART outcomes. Results The medians (interquartile range (IQR)) were as follows: baseline serum folate, 20.55 ng/ml (10.8, 32.9); Mg, 19.4 mg/L (18.7, 20.7); Ca, 94 mg/L (91.2, 96.4); and Ca/Mg ratio, 4.78 (4.55, 5.02). Women with higher serum folate concentrations (Q4≥33.0 ng/ml) had significantly lower total numbers of oocytes retrieved (adjusted mean (95% CI) 9.2 (7.6-11.3) vs 12.9 (10.9-15.4, p-trend=0.006)) and lower odds ratios (ORs) (95% CI) of 0.12 (0.02, 0.79) for clinical pregnancy and 0.10 (0.01, 0.70) for live birth compared with women in the lowest quartile (<10.8 ng/ml), all p-trend<0.001. Women in the highest Ca/Mg ratio quartile (≥5.02) had ORs (95% CI) of 6.58 (1.31, 33.04) for biochemical pregnancy, 4.85 (1.02, 23.08) for clinical pregnancy and 4.07 (0.83, 19.9) for the live birth rate compared with women in the lowest quartile (<4.55), all p-trend<0.001. Conclusions Using multivariable models, we suggested that a baseline elevated serum folate level (≥33.0 ng/ml) and a lower Ca/Mg ratio were associated with worse ART outcomes in normogonadotropic women. Our findings might be useful for choosing safe dosages of folate, calcium, magnesium and complex supplementation for both fertile women and women undergoing infertility treatment. Further preconception large-scale studies with known micro- and macronutrient statuses of both parents and serum folate, Ca, Mg, and hormone levels, are needed.
Penumbra Detection With Oxygen Extraction Fraction Using Magnetic Susceptibility in Patients With Acute Ischemic Stroke

Background The oxygen extraction fraction (OEF) has been applied to identify ischemic penumbral tissue, but is difficult to use in an urgent care setting. This study aimed to investigate whether an OEF map generated via magnetic resonance quantitative susceptibility mapping (QSM) could help identify the ischemic penumbra in patients with acute ischemic stroke. Materials and Methods This prospective imaging study included 21 patients with large anterior circulation vessel occlusion who were admitted <24 h after stroke onset and 21 age-matched healthy controls. We identified the ischemic penumbra as the region with a Tmax of >6 s during dynamic susceptibility contrast-magnetic resonance imaging (DSC-MRI) and calculated the perfusion-core mismatch ratio between the ischemic penumbra and infarct core volumes. The OEF values were measured based on magnetic susceptibility differences between the venous structures and brain tissues using rapid QSM acquisition. Volumes with increased OEF values were compared to the ischemic penumbra volumes using an anatomical template. Results Eleven patients had a perfusion-core mismatch ratio of ≥1.8, and reperfusion therapy was recommended. In these patients, the volumes with increased OEF values of >51.5%, which was defined using the anterior circulation territory OEF values from the 21 healthy controls, were positively correlated with the ischemic penumbra volumes (r = 0.636, 95% CI: 0.059 to 0.895, P = 0.035) and inversely correlated with the 30-day change in the National Institutes of Health Stroke Scale scores (r = −0.624, 95% CI: −0.891 to −0.039, P = 0.041). Conclusion Tissue volumes with increased OEF values could predict ischemic penumbra volumes based on DSC-MRI, highlighting the potential of the QSM-derived OEF map as a penumbra biomarker to guide treatment selection in patients with acute ischemic stroke.