Miracle-dz/UniFAR-FTUs huggingface dataset:
Multi-Scale Surface Treatments of Titanium Implants for Rapid Osseointegration: A Review

The propose of this review was to summarize the advances in multi-scale surface technology of titanium implants to accelerate the osseointegration process. The several multi-scaled methods used for improving wettability, roughness, and bioactivity of implant surfaces are reviewed. In addition, macro-scale methods (e.g., 3D printing (3DP) and laser surface texturing (LST)), micro-scale (e.g., grit-blasting, acid-etching, and Sand-blasted, Large-grit, and Acid-etching (SLA)) and nano-scale methods (e.g., plasma-spraying and anodization) are also discussed, and these surfaces are known to have favorable properties in clinical applications. Functionalized coatings with organic and non-organic loadings suggest good prospects for the future of modern biotechnology. Nevertheless, because of high cost and low clinical validation, these partial coatings have not been commercially available so far. A large number of in vitro and in vivo investigations are necessary in order to obtain in-depth exploration about the efficiency of functional implant surfaces. The prospective titanium implants should possess the optimum chemistry, bionic characteristics, and standardized modern topographies to achieve rapid osseointegration.
A BIOSYSTEMATIC STUDY OF THE GENUS ATHALIA LEACH OF JAPAN (HYMENOPTERA : TENTHREDINIDAE)

The Japanese species of the genus Athalia Leach, 1817 are revised. Seven species and one subspecies, i. e. , A. japonica (Klug, 1815), A. infumata (Marlatt, 1898), A. proxima (Klug, 1834), A. rosae rufcornis Jakovlev, 1888, A. kashmirensis Benson, 1932, A. yanoi Takeuchi, 1952, stat. nov. and A. scutellariae Cameron, 1880, are recognized not only from the morphological characters of the adults and larvae including chromosome number but also from the biological knowledge such as host plants, life cycle, natural enemies, distribution, and electrophoretic analysis of the larval hemolymph protein. A. scutellariae is recorded from Japan for the first time. Redescriptions of the adults of all the species in Japan are given. Descriptions of the larvae of all the species except those of A. scutellariae are also given. Larvae of A. kashmirensis and A. yanoi are newly described. Keys to species and subspecies are given both for the adults and larvae.
Binding and Neutralizing Capacity of Respiratory Syncytial Virus (RSV)-Specific Recombinant IgG Against RSV in Human Milk, Gastric and Intestinal Fluids from Infants

Oral administration of pathogen-specific recombinant antibodies may help to prevent infant gastrointestinal (GI) pathogen infection; however, to neutralize an infectious agent, these antibodies must resist degradation in the GI tract. Palivizumab, a recombinant antibody specific for the respiratory syncytial virus (RSV), was used as a model for pathogen-specific IgG in human milk. The aim was to compare the remaining binding capacity of palivizumab in milk between three mothers after exposure to an in vitro model of infant gastrointestinal digestion (gastric and duodenal fluids) using ELISA. The neutralizing capacity of palivizumab in pooled human milk, gastric contents, and stools from preterm infants was also evaluated for blocking RSV with green fluorescent protein (RSV-GFP) infection in Hep-2 cells using confocal and inverted microscopy and flow cytometry. The reduction of palivizumab binding capacity in human milk and digested samples was slightly different between mothers. Overall, palivizumab decreased 50% after simulated gastric digestion with pepsin and 62% after simulated intestinal digestion with pancreatin. Palivizumab (2–8 μg/mL) in human milk or stool samples blocked RSV (3.4 × 104 FFU/mL) infection (no syncytia formation on Hep-2 cells) by microscopy. Syncytia formation was detected on Hep-2 cells when RSV was incubated in gastric contents or virus medium with 2–4 μg/mL of palivizumab, but no infection was observed at 8 μg/mL. No fluorescence (absence of infected cells) was detected when palivizumab (100 μg/mL) was incubated in human milk or medium with RSV-GFP (1.1 × 105 FFU/mL), whereas fluorescence increased with the reduced concentration of palivizumab using flow cytometry. These results suggest that undigested and digested matrices could change the binding and neutralizing capacity of viral pathogen-specific antibodies.
The combined effect of daylength and CO2 on coccolithophore physiology

Atmospheric CO2 levels have been increasing at an accelerated rate for the last 250 years, much of which is absorbed by the oceans, resulting in a process called ocean acidification (OA). This phenomenon has the capacity to disrupt many marine biological processes that utilise carbon, in particular photosynthesis and calcification, and as such phytoplankton have been a main topic of OA studies. While research has accelerated over the last decade, establishing general trends still remains confounded by methodological inconsistencies. Coccolithophores, particularly the species Emiliania huxleyi, are both ecologically and biogeochemically important phytoplankton; however, one strain (NZEH) has produced highly varied results. Here, we present a multivariate analysis that suggests previous inconsistencies between past studies of NZEH may be driven by variance of the light:dark (L:D) cycle used for growth. Experimental analysis on NZEH showed that under a 14:10h L:D cycle, CO2 induces significantly slower growth rates and higher PIC and POC cell-1, but this effect is dampened under 24h of light. This was widened to encompass more taxa, including more isolates of E. huxleyi (PLY70-3, PLY124-3, RCC962), and two other species of coccolithophore; Gephyrocapsa oceanica and Coccolithus pelagicus. L:D cycle changed the observed OA response, with two main responses divided by biogeographical origin. In tropical taxa, 24h light enhanced the effects of increased photosynthesis, but dampened the decrease in calcification in response to CO2. For temperate taxa, 24h dampened both the increases in photosynthesis and calcification with CO2. Evaluation of photobiology reveals that both CO2 and longer photoperiods induce a “high light” acclimation response, and changes in coccosphere thickness suggest it has a photoprotective role. Finally, results from bioassay experiments on natural phytoplankton populations in the polar regions show that CO2 response is hard to predict and based on community composition and ambient starting conditions. This work serves to further highlight the importance of environmental variables that moderate the OA response in accurately understanding future biogeochemical cycles. Future models attempting to predict the impact of OA upon marine systems must critically account for interactive role of light availability.
Association of Allostatic Load with All-Cause and Cancer Mortality by Race and Body Mass Index in the REGARDS Cohort

Among 29,701 Black and White participants aged 45 years and older in the Reasons for Geographic and Racial Difference in Stroke (REGARDS) study, allostatic load (AL) was defined as the sum score of established baseline risk-associated biomarkers for which participants exceeded a set cutoff point. Cox proportional hazard regression was utilized to determine the association of AL score with all-cause and cancer-specific mortality, with analyses stratified by body-mass index, age group, and race. At baseline, Blacks had a higher AL score compared with Whites (Black mean AL score: 2.42, SD: 1.50; White mean AL score: 1.99, SD: 1.39; p < 0.001). Over the follow-up period, there were 4622 all-cause and 1237 cancer-specific deaths observed. Every unit increase in baseline AL score was associated with a 24% higher risk of all-cause (HR: 1.24, 95% CI: 1.22, 1.27) and a 7% higher risk of cancer-specific mortality (HR: 1.07, 95% CI: 1.03, 1.12). The association of AL with overall- and cancer-specific mortality was similar among Blacks and Whites and across age-groups, however the risk of cancer-specific mortality was higher among normal BMI than overweight or obese participants. In conclusion, a higher baseline AL score was associated with increased risk of all-cause and cancer-specific mortality among both Black and White participants. Targeted interventions to patient groups with higher AL scores, regardless of race, may be beneficial as a strategy to reduce all-cause and cancer-specific mortality.
Cultural Differences between U.S. Federal Financial Regulators and Implications for Interagency Collaboration

The problematic collaboration between the Commodity Futures Trading Commission and the Securities and Exchange Commission is a recurrent issue in U.S. financial regulatory reform. For enhanced interagency collaboration, this study deals with the important but neglected issue of cultural differences between the agencies, departing from the typical structural and legal viewpoints. As the result of a qualitative analysis, the study found substantive cultural differences between the agencies in terms of three categories: priority of mission, attention to information disclosure, and dominant professional culture. This finding implies that the limited collaboration between the agencies probably has resulted from their cultural differences, and that such cultural differences should be reconciled to improve collaboration in the future.
Towards a Secure Thermal-Energy Aware Routing Protocol in Wireless Body Area Network Based on Blockchain Technology

The emergence of biomedical sensor devices, wireless communication, and innovation in other technologies for healthcare applications result in the evolution of a new area of research that is termed as Wireless Body Area Networks (WBANs). WBAN originates from Wireless Sensor Networks (WSNs), which are used for implementing many healthcare systems integrated with networks and wireless devices to ensure remote healthcare monitoring. WBAN is a network of wearable devices implanted in or on the human body. The main aim of WBAN is to collect the human vital signs/physiological data (like ECG, body temperature, EMG, glucose level, etc.) round-the-clock from patients that demand secure, optimal and efficient routing techniques. The efficient, secure, and reliable designing of routing protocol is a difficult task in WBAN due to its diverse characteristic and restraints, such as energy consumption and temperature-rise of implanted sensors. The two significant constraints, overheating of nodes and energy efficiency must be taken into account while designing a reliable blockchain-enabled WBAN routing protocol. The purpose of this study is to achieve stability and efficiency in the routing of WBAN through managing temperature and energy limitations. Moreover, the blockchain provides security, transparency, and lightweight solution for the interoperability of physiological data with other medical personnel in the healthcare ecosystem. In this research work, the blockchain-based Adaptive Thermal-/Energy-Aware Routing (ATEAR) protocol for WBAN is proposed. Temperature rise, energy consumption, and throughput are the evaluation metrics considered to analyze the performance of ATEAR for data transmission. In contrast, transaction throughput, latency, and resource utilization are used to investigate the outcome of the blockchain system. Hyperledger Caliper, a benchmarking tool, is used to evaluate the performance of the blockchain system in terms of CPU utilization, memory, and memory utilization. The results show that by preserving residual energy and avoiding overheated nodes as forwarders, high throughput is achieved with the ultimate increase of the network lifetime. Castalia, a simulation tool, is used to evaluate the performance of the proposed protocol, and its comparison is made with Multipath Ring Routing Protocol (MRRP), thermal-aware routing algorithm (TARA), and Shortest-Hop (SHR). Evaluation results illustrate that the proposed protocol performs significantly better in balancing of temperature (to avoid damaging heat effect on the body tissues) and energy consumption (to prevent the replacement of battery and to increase the embedded sensor node life) with efficient data transmission achieving a high throughput value.
Inversion Recovery Susceptibility Weighted Imaging With Enhanced T2 Weighting at 3 T Improves Visualization of Subpial Cortical Multiple Sclerosis Lesions

Supplemental digital content is available in the text. Objectives Cortical demyelination is common in multiple sclerosis (MS) and can be extensive. Cortical lesions contribute to disability independently from white matter lesions and may form via a distinct mechanism. However, current magnetic resonance imaging methods at 3 T are insensitive to cortical, and especially subpial cortical, lesions. Subpial lesions are well seen on T2*-weighted imaging at 7 T, but T2*-weighted methods on 3 T scanners are limited by poor lesion-to-cortex and cerebrospinal fluid-to-lesion contrast. We aimed to develop and evaluate a cerebrospinal fluid–suppressed, T2*-weighted sequence optimized for subpial cortical lesion visualization. Materials and Methods We developed a new magnetic resonance imaging sequence, inversion recovery susceptibility weighted imaging with enhanced T2 weighting (IR-SWIET; 0.8 mm × 0.8 mm in plane, 0.64 mm slice thickness with whole brain coverage, acquisition time ~5 minutes). We compared cortical lesion visualization independently on IR-SWIET (median signal from 4 acquisitions), magnetization-prepared 2 rapid acquisition gradient echoes (MP2RAGE), double inversion recovery (DIR), T2*-weighted segmented echo-planar imaging, and phase-sensitive inversion recovery images for 10 adults with MS. We also identified cortical lesions with a multicontrast reading of IR-SWIET (median of 2 acquisitions), MP2RAGE, and fluid-attenuated inversion recovery (FLAIR) images for each case. Lesions identified on 3 T images were verified on “gold standard” 7 T T2* and MP2RAGE images. Results Cortical, and particularly subpial, lesions appeared much more conspicuous on IR-SWIET compared with other 3 T methods. A total of 101 true-positive subpial lesions were identified on IR-SWIET (average per-participant sensitivity vs 7 T, 29% ± 8%) versus 36 on MP2RAGE (5% ± 2%; comparison to IR-SWIET sensitivity, P = 0.07), 17 on FLAIR (2% ± 1%; P < 0.05), 28 on DIR (6% ± 2%; P < 0.05), 42 on T2*-weighted segmented echo-planar imaging (11% ± 5%; P < 0.05), and 13 on phase-sensitive inversion recovery (4% ± 2%; P < 0.05). When a combination of IR-SWIET, MP2RAGE, and FLAIR images was used, a total of 147 subpial lesions (30% ± 5%) were identified versus 83 (16% ± 3%, P < 0.01) on a combination of DIR, MP2RAGE, and FLAIR. More cases had at least 1 subpial lesion on IR-SWIET, and IR-SWIET improved cortical lesion subtyping accuracy and correlation with 7 T subpial lesion number. Conclusions Subpial lesions are better visualized on IR-SWIET compared with other 3 T methods. A 3 T protocol combining IR-SWIET with MP2RAGE, in which leukocortical lesions are well seen, improves cortical lesion visualization over existing approaches. Therefore, IR-SWIET may enable improved MS diagnostic specificity and a better understanding of the clinical implications of cortical demyelination.
Recent Developments in Ozone Sensor Technology for Medical Applications

There is increasing interest in the utilisation of medical gases, such as ozone, for the treatment of herniated disks, peripheral artery diseases, and chronic wounds, and for dentistry. Currently, the in situ measurement of the dissolved ozone concentration during the medical procedures in human bodily liquids and tissues is not possible. Further research is necessary to enable the integration of ozone sensors in medical and bioanalytical devices. In the present review, we report selected recent developments in ozone sensor technology (2016–2020). The sensors are subdivided into ozone gas sensors and dissolved ozone sensors. The focus thereby lies upon amperometric and impedimetric as well as optical measurement methods. The progress made in various areas—such as measurement temperature, measurement range, response time, and recovery time—is presented. As inkjet-printing is a new promising technology for embedding sensors in medical and bioanalytical devices, the present review includes a brief overview of the current approaches of inkjet-printed ozone sensors.
Genotypic Variation of Total Phenol and Oleuropein Concentration and Antioxidant Activity of 11 Greek Olive Cultivars (Olea europaea L.)

The chemical and biochemical composition of olives relies on some agronomical factors, one of which is the cultivar. In this study, fruits and leaves of 11 Greek olive cultivars were examined concerning their phenol and oleuropein concentrations. Fruit antioxidant activity was determined as well. The obtained results showed that significant differences existed among cultivars regardless of the tissue or the measured parameter. In general, leaves had higher total phenol and oleuropein concentrations than fruits. Finally, the highest oleuropein concentration in fruits was recorded in 'Pikrolia Kerkiras' followed by 'Romeiki', 'Megaritiki', 'Kothreiki', and 'Kalamon'. These cultivars may constitute the raw material in the industrial production of oleuropein. Experimental olive orchard. The study was carried out in a field planted with 11 Greek olive cultivars, namely 'Kalamon', 'Kothreiki', 'Maronia', 'Megaritiki', 'Petrolia', 'Pikrolia Kerkiras', 'Romeiki', 'Smertolia', 'Thassitiki', 'Throubolia Aegean', and 'Valanolia' (seven trees per cultivar and of the same age and vegetative habits), during the 2009-2010 sea- son. These 10-year-old olive cultivars were grown in the same orchard located in Poros Troizinia, Northeast Peloponnese, Greece (lat. 37� 31# N, long. 23� 29# E). The climate of the area is Mediterranean with mild winters (9-10 � C) and dry, hot summers (27-28 � C). The mean annual precipitation of the area is 550 mm. The olive plants were subjected to the same cultural conditions (pruning, fertil- ization, irrigation, and harvesting). For each cultivar, a 3-kg composite sample of fruits and 1 kg of current-season leaves were collected from all the orientations of the tree canopy on 15 Nov. Each composite sample (fruit or leaf) was divided into three subsamples of 200 g each. Maturity index. The maturity index of
Colpomenia wynnei sp. nov. (Scytosiphonaceae, Phaeophyceae): a new species of marine algae from northeast Asia

Abstract: The taxonomic distinctiveness and distribution of the brown alga Colpomenia phaeodactyla are debatable. The type locality of this species is Puerto Peñasco, Mexico, and its distribution includes northeast Asia. We analyzed two gene sequences of specimens of so-called C. phaeodactyla from Korea, Japan and Mexico, in combination with morphological observations of the specimens. Analyses of 71 cox3 and 23 rbcL sequences resulted in similar trees in which specimens under the name C. phaeodactyla consisted of two distinct clades. One clade comprised samples from Korea and Japan, and the other clade, from the Gulf of California, was identical to C. durvillei. Additional observations of the morphology and anatomy of the specimens agreed with the molecular data. On the basis of these results, we describe the new species C. wynnei for plants from Korea and Japan. Colpomenia wynnei was distinguished by finger-like sacs with adventitious branchlets when mature and was much contorted, the height of ascocysts being shorter than plurilocular sporangia. Both cox3 and rbcL sequence data strongly supported the monophyly of C. wynnei, C. bullosa and C. durvillei, all being characterized by having elongate thalli. Based on both cox3 and rbcL data sets, we herein propose to synonymise C. phaeodactyla with C. durvillei.
Facial Skin Temperature and Discomfort When Wearing Protective Face Masks: Thermal Infrared Imaging Evaluation and Hands Moving the Mask

Individual respiratory protective devices and face masks represent critical tools in protecting health care workers in hospitals and clinics, and play a central role in decreasing the spread of the high-risk pandemic infection of 2019, coronavirus disease (COVID-19). The aim of the present study was to compare the facial skin temperature and the heat flow when wearing medical surgical masks to the same factors when wearing N95 respirators. A total of 20 subjects were recruited and during the evaluation, each subject was invited to wear a surgical mask or respirator for 1 h. The next day in the morning at the same hour, the same subject wore a N95 mask for 1 h with the same protocol. Infrared thermal evaluation was performed to measure the facial temperature of the perioral region and the perception ratings related to the humidity, heat, breathing difficulty, and discomfort were recorded. A significant difference in heat flow and perioral region temperature was recorded between the surgical mask and the N95 respirator (p < 0.05). A statistically significant difference in humidity, heat, breathing difficulty, and discomfort was present between the groups. The study results suggest that N95 respirators are able to induce an increased facial skin temperature, greater discomfort and lower wearing adherence when compared to the medical surgical masks.
Determination of Peptide Substrate Specificity for μ-Calpain by a Peptide Library-based Approach

Calpains are proteases that catalyze the limited cleavage of target proteins in response to Ca2+ signaling. Because of their involvement in pathological conditions such as post-ischemic injury and Alzheimer and Parkinson disease, calpains form a class of pharmacologically significant targets for inhibition. We have determined the sequence preference for the hydrolysis of peptide substrates of the ubiquitous μ-calpain isoform by a peptide library-based approach using the proteolytic core of μ-calpain (μI-II). The approach, first described by Turk et al. (Turk, B. E., Huang, L. L., Piro, E. T., and Cantley, L. C. (2001) Nat. Biotechnol. 19, 661–667), involved the digestion of an N-terminally acetylated degenerate peptide library in conjunction with Edman sequencing to determine the specificity for residues found at primed positions. The cleavage consensus for these positions was then used to design a second, partially degenerate library, to determine specificity at unprimed positions. We have improved upon the original methodology by using a degenerate peptide dendrimer for determination of specificity at unprimed positions. By using this modified approach, the complete cleavage specificity profile for μI-II was determined for all positions flanking the cleaved peptide. A previously known preference of calpains for hydrophobic amino acids at unprimed positions was confirmed. In addition, a novel residue specificity for primed positions was revealed to highlight the importance of these sites for substrate recognition. The optimal primed site motif (MER) was shown to be capable of directing cleavage to a specific peptide bond. Accordingly, we designed a fluorescent resonance energy transfer-based substrate with optimal cleavage motifs on the primed and non-primed sides (PLFAER). The μ-calpain core shows a far greater turnover rate for our substrate than for those based on the cleavage site of α-spectrin or the proteolytic sequence consensus compiled from substrate alignments.
In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes

Summary The four RNA species of a French strain of beet necrotic yellow vein virus (BNYVV) have lengths of 7100, 4800, 1800 and 1500 nucleotides, as measured by agarose gel electrophoresis in denaturing conditions. Translation of purified RNA in a reticulocyte cell-free system revealed that RNA-2 encodes the 22000 mol. wt. coat protein. Cloned cDNA copies of each of the four viral RNA species have been used to show that BNYVV RNA-3 and RNA-4 share no sequence homology with RNA-1 and RNA-2. Thus, RNA-3 and RNA-4 cannot be subgenomic messengers derived from the larger RNA species.