Miracle-dz/UniFAR-FTUs huggingface dataset:
Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in Mice

Secreted protein acidic and rich in cysteine (SPARC) is a matricellular glycoprotein implicated in various functions, including metabolism, tissue regeneration, and functional homeostasis. SPARC/Sparc declines with ageing but increases with exercise. We aim to verify two hypotheses: (1) SPARC deficiency leads to an ageing-like phenotype (metabolic decline, muscle loss, etc.), and (2) SPARC overexpression would mimic exercise, counteract ageing, and improve age-related changes. Our mice experiments are divided into two parts. First, we explore the consequences of Sparc knockout (KO) and compare them to the ageing effects. We also observe the effects of exercise. In the second part, we study the effects of SPARC overexpression and compare them to the exercise benefits. At the end, we make an analysis of the results to point out the analogies between Sparc KO and the ageing-like phenotype on the one hand and make comparisons between SPARC overexpression and exercise in the context of exercise counteracting ageing. The measurements were mainly related to tissue weights, adiposity, metabolism, and muscle strength. The main findings are that Sparc KO reduced glucose tolerance, muscle glucose transporter expression, and abdominal adipose tissue weight but increased glycogen content in the muscle. SPARC overexpression increased muscle strength, muscle mass, and expressions of the muscle glucose transporter and mitochondrial oxidative phosphorylation but lowered the glycemia and the adiposity, especially in males. Collectively, these findings, and the data we have previously reported, show that Sparc KO mice manifest an ageing-like phenotype, whereas SPARC overexpression and exercise generate similar benefits. The benefits are towards counteracting both the SPARC deficiency-induced ageing-like phenotype as well as reversing the age-related changes. The potential applications of these findings are to build/optimize Sparc KO-based animal models of various health conditions and, on the other hand, to develop therapies based on introducing SPARC or targeting SPARC-related pathways to mimic exercise against age-related and metabolic disorders.
Orientation-Insensitive Chipless RFID Achieved in Multiple Rotational Degrees of Freedom

Frequency-coded chipless radiofrequency (RFID) suffers from resonant frequency detuning caused by misaligned orientations. The literature has employed topological symmetry to prevent shape variations; however, this scheme is effective only for the orientation in roll and cannot address other rotational degrees of freedom including pitch and yaw. This study takes the first step to cope with the orientation mismatch against arbitrary roll, pitch, and yaw angles. The proposed technique requires the design of tag and new reader architecture, eliminating complex signal processing. The tag comprises resonators with the pattern of a short dipole. Two reader antennas are placed orthogonally and fed with equal power, illuminating co-polarized electromagnetic waves toward the tag. Based on the Pythagorean trigonometric identity, the sum of the backscattering power is unvarying against the rotations in pitch or yaw. The proposed technique is experimentally validated using a 6.97-bit system. When orientation mismatch in yaw appears, the proposed scheme improves the read reliability from 54.3% to 93.5%. Moreover, when mismatch in both roll and yaw occurs, the reliability is improved from 43.8% to 91.8%. Practical aspects including various detection zones and the loading effect are also analyzed.
Does Self-Efficacy have a Relationship with Career Aspiration? A Study among Secondary School Students in Anambra State

Aims: In Anambra state, Nigeria, making a choice of career has remained a constant challenge for many students, especially those in the senior secondary who are on the verge of making a choice of career. This study investigated academic self-efficacy as predictor of achievement career aspiration of secondary school students in Anambra State, Nigeria. Two research questions guided the study while two null hypotheses were tested at 0.05 level of significance. Methodology: The study adopted a correlation research design. The study was carried out in Anambra State. The sample 2,700 SS1 and SS 2 students were selected from a population of 32,450 senior secondary school students. Two research instruments were used for data collection. These are: Career Aspiration Scale (CAS) and Academic Self-Efficacy Scale (ASES). The internal consistency reliability of the instruments was established using Cronbach Alpha statistics. The results are as follows: CAS 0.891; ASES 0.842. Copies of the questionnaire were distributed and retrieved through direct delivery method. Data collected were analysed using Statistical Package for Social Science (SPSS). Simple linear regression analysis was used in answering research questions and testing the hypotheses. Results: The finding of the study revealed among others that academic self-efficacy of the secondary school students is not a significant predictor of their career aspiration. Conclusion: Based on the findings of the study, it was concluded that academic self-efficacy of secondary school students is not a significant predictor of their achievement career, leaderships and educational aspiration. It was recommended that Guidance Counsellors working in secondary schools should organise workshops that will be geared towards enhancing students’ academic self-efficacy and help them in aspiring for careers that are in line with their academic self-efficacy.
Development and Validation of HPLC-DAD Method with Pre-Column PMP Derivatization for Monomeric Profile Analysis of Polysaccharides from Agro-Industrial Wastes

The instrumental analysis of complex mixtures of sugars often requires derivatization to enhance the method’s selectivity and sensitivity. 1-Phenyl-3-methyl-5-pyrazolone (PMP) is a common sugar derivatization agent used in high-performance liquid chromatography (HPLC). Although many C18 column applications for PMP–sugar derivative analysis have been developed, their transferability is not straightforward due to variations in column chemistry and preparation technology. The aim of this study was to develop and validate an application for Zorbax Extend C18 columns for the analysis of 8 neutral and 2 acidic sugars commonly found in plant polysaccharides. The method was further compared to well-established alditol acetates and m-hydroxydiphenyl methods and employed for sugar profiling of selected agro-industrial wastes. The most influential separation factors were the mobile-phase pH and acetonitrile content, optimized at 8.0 and a 12–17% gradient, respectively. The method showed excellent linearity, repeatability and intermediate precision. High sensitivity was achieved, especially for neutral sugars, with an accuracy error range of 5–10% relative standard deviation. The sugar profiling results were highly correlated to the reference method for neutral sugars. The HPLC method was highly applicable for the evaluation of polysaccharides in selected wastes and showed advantages in terms of simplicity, accuracy in acidic sugar determination and suitability for their simultaneous analysis with neutral sugars.
Effect of Peroxyl Radical-Induced Oxidation on Functional and Structural Characteristics of Walnut Protein Isolates Revealed by High-Resolution Mass Spectrometry

The present study aims to investigate the structural and functional properties of oxidated walnut protein isolates (WPI) by 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH). The oxidation degree, changes in structural characteristics, processing properties, and protein modifications of WPI were measured. The results showed that oxidation significantly induced structural changes, mainly reflected by the increasing carbonyl content, and decreasing sulfhydryl and free amino groups. Moreover, the secondary structure of WPI was altered in response to oxidation, and large aggregates formed through disulfide cross-linking and hydrophobic interactions. Almost all the property indicators were significantly decreased by oxidation except the foaming property and water/oil holding capacity. Mass spectrometry analysis showed that 16 different modifications occurred in amino acid side chains, and most of the protein groups with higher numbers of modifications were found to be associated with allergies, which was further confirmed by the reduction in antigenicity of the major allergen (Jug r 1) in WPI. Meanwhile, we used oxidation-related proteins for gene ontology (GO) enrichment analyses, and the results indicated that 115, 204 and 59 GO terms were enriched in terms of biological process, molecular function, and cellular component, respectively. In conclusion, oxidation altered the groups and conformation of WPI, which in turn caused modification in the functional properties correspondingly. These findings might provide a reference for processing and storage of walnut protein foods.
Physicochemical, Structural, Thermal and Rheological Properties of Flour and Starch Isolated from Avocado Seeds of Landrace and Hass Cultivars

The objective of this study was to obtain and characterize flours and starches from the avocado seeds of Hass and landrace cultivars. The morphological, physical-chemical, structural, thermal and rheological characteristics were evaluated. The flour yield of the Hass and landrace cultivars was 41.56 to 46.86% (w/w), while for starch, it was 35.47 to 39.57% (w/w) (cv. Hass and landrace, respectively). Scanning electron microscopy (SEM) revealed the presence of oval starch granules and other particles in flour, in contrast to flours, starches showed lower ash, proteins and lipids content. However, the amylose content was higher in starches (42.25–48.2%). Flours showed a higher gelatinization temperature (Tp = 73.17–73.62 °C), and their starches presented greater gelatinization enthalpy (∆Hgel = 11.82–13.43 J/g). All samples showed a B-type diffraction pattern, and the crystallinity was higher in the flours. The rheological analysis (flow curves and viscoelastic tests) evidenced a pseudoplastic (n = 0.28–0.36) behavior in all samples analyzed, but the consistency index (k) was higher in starches. In general, the flours and starches from avocado seeds presented interesting proximal, thermal and functional properties for possible application in food systems, and these findings could contribute to the revaluation of this by-product.
Final Report of the ModSysC2020 Working Group - Data, Models and Theories for Complex Systems: new challenges and opportunities

At University Montpellier 2, the modeling and simulation of complex systems has been identified as a major scientific challenge and one of the priority axes in interdisciplinary research, with major potential impact on training, economy and society. Many research groups and laboratories in Montpellier are already working in that direction, but typically in isolation within their own scientific discipline. Several local actions have been initiated in order to structure the scientific community with interdisciplinary projects, but with little coordination among the actions. The goal of the ModSysC2020 (modeling and simulation of complex systems in 2020) working group was to analyze the local situation (forces and weaknesses, current projects), identify the critical research directions and propose concrete actions in terms of research projects, equipment facilities, human resources and training to be encouraged. To guide this perspective, we decomposed the scientific challenge into four main themes, for which there is strong background in Montpellier: (1) modeling and simulation of complex systems; (2) algorithms and computing; (3) scientific data management; (4) production, storage and archiving of data from the observation of the natural and biological media. In this report, for each theme, we introduce the context and motivations, analyze the situation in Montpellier, identify research directions and propose specific actions in terms of interdisciplinary research projects and training. We also provide an analysis of the socio-economical aspects of modeling and simulation through use cases in various domains such as life science and healthcare, environmental science and energy. Finally, we discuss the importance of revisiting students training in fundamental domains such as modeling, computer programming and database which are typically taught too late, in specialized masters.
Sex-specific regulation of binge drinking, social, and arousal behaviors by subcortical serotonin 5HT2c receptor-containing neurons

Serotonin 5HT2c receptors have been implicated in the pathophysiology of both mood disorders and alcohol use disorder, but the circuits mediating the effects of systemic pharmacological manipulations of this receptor on behavior have not been identified. Binge alcohol consumption induces discrete social and arousal disturbances in human populations, which are thought to promote increased drinking. However, whether models of binge drinking in rodents can induce these same long-term negative behavioral symptoms is unknown. In this study, we employed multiple anatomical, physiological, and behavioral approaches to identify two populations of neurons expressing serotonin 5HT2c receptors, one in the lateral habenula (LHb5HT2c) and one in the bed nucleus of the stria terminalis (BNST5HT2c), that display coordinated in-vivo responses to social, arousal, and alcohol-related stimuli and are physiologically modulated by binge alcohol consumption in a sex-specific manner. Critically, these physiological changes were associated with sex-specific behavioral disturbances that closely mirror social and arousal symptoms observed in humans during abstinence from binge drinking. Specifically, we observed that one week of abstinence from three weeks of binge alcohol drinking induced social recognition deficits in females and potentiated acoustic startle responses in males. While both populations of neurons (BNST and LHb) and the 5HT2c receptor itself contribute to the sex-specific effects of alcohol on social and arousal behaviors to some degree, the primary causal mechanism underlying these phenomena appears to be excessive activation of LHb5HT2c neurons. These findings may have implications for the development of sex-specific treatments for mood and alcohol use disorders targeting the brain’s serotonin system.
Autonomous Area Mapping with Low Cost Hovercraft Platform

In this paper, the possibility to build a low cost hovercraft platform with easily accessible materials and able to perform autonomous ground mapping is discussed. To do so, platform design is presented with proportional motors and construction module. The computing system is integrated to manage the platform displacement. Algorithms are used to counteract every constraint existing in this kind of vehicle such as low ground interaction and motors feedback, based on previous research on hovercraft stabilization. The mapping system which includes a camera and a laser is discussed in detail, and final mapping and exploration is computed with inspiration from mapping research.
Advanced Glycation End-Products (AGEs) of Lysine and Effects of Anti-TCR/Anti-TNF-α Antibody-Based Therapy in the LEW.1AR1-iddm Rat, an Animal Model of Human Type 1 Diabetes

The LEW.1AR1-iddm rat is an animal model of human type 1 diabetes (T1D). Previously, we have shown that combination with anti-TCR/anti-TNF-α antibody-based therapy re-established normoglycemia and increased proteinic arginine-dimethylation in the spleen, yet not in the pancreas. High blood glucose is often associated with elevated formation of advanced glycation end-products (AGEs) which act via their receptor (RAGE). Both anti-TCR and anti-TNF-α are inhibitors of RAGE. The aim of the present work was to investigate potential biochemical changes of anti-TCR/anti-TNF-α therapy in the LEW.1AR1-iddm rat. We determined by stable-isotope dilution gas chromatography-mass spectrometry (GC-MS) the content of free and proteinic AGEs and the Nε-monomethylation of lysine (Lys) residues in proteins of pancreas, kidney, liver, spleen and lymph nodes of normoglycemic control (ngCo, n = 6), acute diabetic (acT1D, n = 6), chronic diabetic (chT1D, n = 4), and cured (cuT1D, n = 4) rats after anti-TCR/anti-TNF-α therapy. Analyzed biomarkers included Lys and its metabolites Nε-carboxymethyl lysine (CML), furosine and Nε-monomethyl lysine (MML). Other amino acids were also determined. Statistical methods including ANOVA, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to evaluate the effects. Most statistical differences between the study groups were observed for spleen, pancreas and kidney, with liver and lymph nodes showing no such differences. In the pancreas, the groups differed with respect to proteinic furosine (p = 0.0289) and free CML (p = 0.0023). In the kidneys, the groups differed with respect to proteinic furosine (p = 0.0076) and CML (p = 0.0270). In the spleen, group differences were found for proteinic furosine (p = 0.0114) and free furosine (p = 0.0368), as well as for proteinic CML (p = 0.0502) and proteinic MML (p = 0.0191). The acT1D rats had lower furosine, CML and MML levels in the spleen than the rats in all other groups. This observation corresponds to the lower citrullination levels previously measured in these rats. PCA revealed diametric associations between PC1 and PC2 for spleen (r = −0.8271, p < 0.0001) compared to pancreas (r = 0.5805, p = 0.0073) and kidney (r = 0.8692, p < 0.0001). These findings underscore the importance of the spleen in this animal model of human T1D. OPLS-DA showed that in total sixteen amino acids differed in the experimental groups.
Temporal Computing Resource Allocation Scheme With End Device Assistance

With the development of the Industrial Internet of Things (IIoT) and edge computing technology, edge servers have been used to allocate computing resources to IIoT devices for alleviating the huge computing needs of IIoT applications. Aiming at the problems of unbalanced distribution and inefficient use of computing resources during computing resource allocation, we propose a temporal computing resource allocation scheme with end device assistance. First, by establishing the temporal interest degree which uses the long-term interest accumulation and short-term interest change, IIoT devices are divided into different interest queues, the end devices with similarly temporal interest to the resource request device are sensed. Second, a social RippleNet based on a temporal knowledge graph is constructed. By analyzing the temporal characteristics and propagation process of social attributes in RippleNet, the temporally social similarity between the end devices and the resource request device is extracted, then the final computing resource allocation decision is generated by using the computing resource selection algorithm we proposed based on temporally social similarity. The simulation results show that the scheme we proposed can effectively improve the efficiency of computing resource allocation and balance the distribution of computing resources in the IIoT environment.
A 2.4 GHz high precision local positioning system based on cooperative roundtrip time of flight ranging

In this work, a novel high precision local positioning system is presented. The system is capable of determining the two- or three-dimensional position of a localization unit relative to a set of wireless active transponders, which are mounted at defined anchor positions in a range of up to 200 meters. Operating in the 2.4 GHz ISM band at a bandwidth of approximately 60 MHz, the localization unit carries out distance measurements to the transponders and then calculates its own position in space by the use of a multilateration algorithm. The ranging measurements are based on a cooperative roundtrip time of flight estimation, using amplitude modulated pseudo-noise sequences for pulse compression and a time division multiple access scheme to support multiple transponders in a single measurement cycle. In contrast to multilateration systems based on the time difference of arrival, our roundtrip time of flight system does not need synchronization by wires or fibers between the anchor nodes. This makes setup and operation as easy as placing the transponders and their power supply at precisely known positions in the field. In this paper, the system setup and the used algorithms are described, and an evaluation of the performance is given from outdoor field tests, comparing the position estimates to those of a commercial differential GPS system with inertial sensor support. The raw, untracked positioning results show an accuracy in the decimeter region with seven anchor transponders in a field of 180 x 200 meters.
Evaluation of Thiol/Disulfide Homeostasis in Bronchiectasis

Purpose Thiols are sulfhydryl-containing organic compounds that have an important role in preventing cellular oxidative stress. This study compares the blood oxidative stress marker levels in bronchiectasis cases during their stable periods with healthy controls. Materials and Methods Seventy-seven patients (49 patients with stable bronchiectasis/28 healthy controls), followed up by the chest disease clinic, were included in the study. Peripheral blood thiol-disulfide parameters (NT: native thiol (−SH); TT: total thiol (−SH + SS); SS: disulfide (−SS); SS-SH: disulfide/native thiol index; SS-TT: disulphide/total thiol index; SH-TT: native thiol/total thiol index), and ischemia-modified albumin (IMA) levels were examined in the stable bronchiectasis group and the control group. Thiol-disulfide homeostasis was evaluated using a novel and automated assay. Findings and Result. Blood native thiol levels in patients with stable bronchiectasis were found to be significantly higher compared with healthy controls. A positive correlation between the total airway disease score and IMA levels was present. Our findings revealed that native thiol levels, which constitute a part of the antioxidant defense system, are increased in patients with stable bronchiectasis.
Genome-Wide Association Reveals Trait Loci for Seed Glucosinolate Accumulation in Indian Mustard (Brassica juncea L.)

Glucosinolates (GSLs) are sulphur- and nitrogen-containing secondary metabolites implicated in the fitness of Brassicaceae and appreciated for their pungency and health-conferring properties. In Indian mustard (Brassica juncea L.), GSL content and composition are seed-quality-determining traits affecting its economic value. Depending on the end use, i.e., condiment or oil, different GSL levels constitute breeding targets. The genetic control of GSL accumulation in Indian mustard, however, is poorly understood, and current knowledge of GSL biosynthesis and regulation is largely based on Arabidopsis thaliana. A genome-wide association study was carried out to dissect the genetic architecture of total GSL content and the content of two major GSLs, sinigrin and gluconapin, in a diverse panel of 158 Indian mustard lines, which broadly grouped into a South Asia cluster and outside-South-Asia cluster. Using 14,125 single-nucleotide polymorphisms (SNPs) as genotyping input, seven distinct significant associations were discovered for total GSL content, eight associations for sinigrin content and 19 for gluconapin. Close homologues of known GSL structural and regulatory genes were identified as candidate genes in proximity to peak SNPs. Our results provide a comprehensive map of the genetic control of GLS biosynthesis in Indian mustard, including priority targets for further investigation and molecular marker development.