Miracle-dz/UniFAR-FTUs huggingface dataset:
Au nanorods/ g-C3N4 composite based biosensor for electrochemical detection of chronic lymphocytic leukemia

Objective: With the increasing incidence of cancer and the dramatic effect of early detection on treatment and increase patient's life, many efforts have been devoted to making sensitive diagnosis systems. DNA as a biomarker for diagnosis of different types of cancers at the early stages of illness has attracted much attention.Methods: In this research novel electrochemical biosensor was developed using Pb2+ modified titanium phosphate nanoparticles and two DNA as capture probes. Considerable amounts of lead ions were mounted on the surface of titanium phosphate which produced the electrochemical signal. The surface of the biosensor electrode was modified by Au nanorods/ g-C3N4 composite. The functional group on the surface of g-C3N4, the chemical composition of tip, the morphology of composite and elemental composition of the composite were investigated by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-Ray Spectroscopy (EDS), respectively.Results: The peaks of C, N, and Au were observed in the EDS spectrum. The presence of Au peaks in the EDS spectrum confirmed the formation of composite from Au nanorods and g-C3N4 sheets. Whit this biosensor the linear range from 0.6 nM to 6.4 nM and the detection limit of 20 PM for target DNA was obtained.Conclusion: Finally, it seems that the glassy carbon electrode modified with Au-nanorods/g-C3N4 composite is a good candidate for cancer diagnosis in the early stages.
Effects of cold storage on Thripobius javae (=T. semiluteus) (Hymenoptera: Eulophidae)

Abstract Thripobius javae (Girault) was introduced in 1995 from Israel into Italy to control the greenhouse thrips, Heliothrips haemorrhoidalis (Bouché). Following introduction, successive augmentative releases of this parasitoid gave unsatisfactory and contradictory results, mainly due to the difficulty in synchronising its availability in sufficient number at the time of release. Efficient storage of this biological control agent could improve its current production and use. The effects of different sets of storage techniques at a single temperature and with a combination of different temperatures and instars on several fitness traits (residual developmental time to adult emergence after the end of storage, pupal mortality, longevity with and without hosts and progeny of emerged adults) were evaluated in order to determine the best conditions for storing the parasitoid. For the pupal stage, increasing storage up to 14 days, at 10°C, gave only a moderate reduction (33%) of a modified composite quality index of its fitness. In contrast, when adults were stored for more than 10 days, at 15°C, residual longevity and progeny were reduced significantly. A combination of two temperatures (10 and 15°C) for pupal storage and a combination of pupal (10°C) and adult (15°C) storage had detrimental effects on parasitoid fitness. Temperatures of storage lower than 15 and 10°C had detrimental effects on adults and pupae, respectively.
ECOLOGY, BEHAVIOR AND BIONOMICS Associação de Constrictotermes cyphergaster Silvestri (Isoptera: Termitidae) com Espécies Arbóreas do Cerrado Brasileiro

Termites usually build nests differently shaped and characterized according to each species, to protect and keep society cohesion. Some species build nests in the ground, some prefer tree thunks or branches as support, whereas other dig galleries in the wood. The purpose of this study is to analyze the relationship between the occurrence of arboreal termites Constrictotermes cyphergaster Silvestri and tree species that support the nest of this species, in a Cerrado sensu strictu of the Serra de Caldas Novas, GO. Data suggest a association relationship between C. Cyphergaster and the tree species Qualea grandiflora Mart. , Annona crassiflora Mart. , Caryocar brasiliense Camb. and Plathymenia reticulata Benth. , shown by high Qui-squared values (χ 2 = 214.986, gl. = 20, P < 0.001). This relationship may be found among other termites and tree species, including Cerrado biome, and may be due to several factors, such as natural competitors and predators, toxin production by other tree species or benefits between associated species (facultative mutualism or facilitation).
Interleukin-1β Induces Cyclooxygenase-2 Gene Expression in Cultured Endometrial Stromal Cells

Increasing evidence indicates that PGs may play an obligatory role in blastocyst implantation. Cyclooxygenase (also known as PGH synthase) isozymes 1 and 2 catalyze the rate-limiting steps in the biosynthesis of PGs. The ubiquitous cyclooxygenase-1 (COX-1) subserves housekeeping functions, whereas the inducible cyclooxygenase-2 (COX-2) is expressed by limited cell types and tightly controlled. Here we report the induction of COX-2 gene expression by interleukin-1β (IL-1β) in cultured human endometrial stromal cells. COX-2 activity was induced by IL-1β (1 ng/mL); conversion of exogenous arachidonic acid to PGF2α increased from 2.6 ± 0.6 ng/well (mean ± sem; n = 6) to 22.2 ± 5.6 ng, but was completely blocked (2.8 ± 0.7 ng/well) by NS-398, a specific COX-2 inhibitor. Undetectable in quiescent stromal cells, messenger ribonucleic acid for COX-2 was induced 30 min after IL-1β treatment, reached a maximum at 4 h, and decreased after 15 h. Protein synthesis was not required for induction of the COX-2 gene, as i.
Variation tolerance for high-speed negative capacitance FinFET SRAM bit cell

Negative capacitance FinFET (NC-FinFET) has a promising developmental prospect due to its superior performance in SS < 60 mV/dec (subthreshold swing), especially in SRAM. Noise margin is an important metric to evaluate the performance for SRAM, together with static leakage, read speed, etc. In this paper, we study the effects of the variation of ferroelectric material (thickness, polarization), FinFET critical physical parameters (fin number, channel length) and some ambient factors (working temperature, supply voltage) on the performance of NC-FinFET SRAM within the reasonable fluctuation tolerance range. The SRAM bit cell is analyzed with a basic 6T structure. The impact of fin number and channel length for NC-FinFET SRAM is different from that of conventional FinFETs. Additionally, the ferroelectric material and some other factors are assessed in detail.
Sharksucker¿shark interaction in two carcharhinid species

It is not known whether sharksuckers have positive or negative effects on their hosts, partly because this association is difficult to study in free-ranging fish. I observed the behaviour of sharks with and without sharksuckers, to determine whether the hosts actively avoid sharksuckers. Wild blacktip sharks, Carcharhinus limbatus, took evasive actions when sharksuckers, Echeneis naucrates, attached to them, presumably to escape from skin irritation or hydrodynamical drag caused by the sharksuckers. Sharksuckers were most often attached to the belly or back of the shark, and sharks reacted most strongly to sharksuckers on their heads, sides, and dorsal fins. Observations of two captive bull sharks, Carcharhinus leucas, indicated that swimming speed increased when sharksuckers were attached. This paper supports the hypothesis that sharksucker attachment irritates sharks, and that the relationship between the two is best viewed as a subtle host–parasite interaction.
DATA MINING FOR PREDICTING THE AMOUNT OF COFFEE PRODUCTION USING CRISP-DM METHOD

The production of coffee plantations has become the leading plantation commodity with the export value of the fourth rank after oil palm, rubber and coconut. The number of coffee needs for export every year always increases, therefore it is necessary to predict the yield of coffee plants to estimate planting and anticipation that will be done so as to achieve the target. Coffee plant productivity is influenced by internal and external factors, namely the quality of the plant itself, soil, altitude and climate. The method used in this study is the CRISPDM method and multiple linear regression algorithm to predict the amount of coffee production and determine the relationship between the variables. The steps taken are business understanding, data understanding, data preparation, modeling and evaluation. The data set that is used as many as 170 data after going through the data preparation stage produced 150 data with 5 attributes in the table. With calculations using tools, the coefficient of determination is 91.96%. That the variation in the value of the production of coffee plants is influenced by independent variables, namely the area of plantations, rainfall, air pressure and solar radiation by 91.96% and 8.04% influenced by other variables not measured in this study. The results of the evaluation and validation of predictions produce good accuracy with an RMSE value of 0.3477. Keyword: data mining, coffee plants, crisp-dm, multiple linear progression. Intisari—Produksi hasil perkebunan kopi menjadi komoditas perkebunan unggulan dengan nilai ekspor urutan ke empat setelah komoditas kelapa sawit, karet, dan kelapa. Jumlah kebutuhan kopi untuk ekspor tiap tahun selalu mengalami peningkatan oleh karena itu diperlukan prediksi hasil produksi tanaman kopi untuk memperkirakan penanaman dan antisipasi yang akan dilakukan sehingga dapat mencapai target. Produktivitas tanaman kopi dipengaruhi oleh faktor internal dan eksternal yaitu kualitas tanaman itu sendiri, tanah, ketinggian dan ilkim. Metode yang digunakan dalam penelitian ini adalah metode CRISP-DM dan algoritma multiple linear regression untuk prediksi jumlah produksi kopi dan mengetahui hubungan antar variabelnya. Tahapan yang dilakukan adalah business understanding, data understanding, data preparation, modeling dan evaluation. Data set yang digunakan sebanyak 170 data setelah melalui tahap data preparation dihasilkan 150 data dengan 5 atribut pada tabel. Dengan perhitungan menggunakan tools dihasilkan koefisien determinasi sebesar 91,96%. Bahwa variasi nilai jumlah produksi tanaman kopi dipengaruhi oleh variabel independen yaitu luas areal perkebunan, curah hujan, tekanan udara dan penyinaran matahari sebesar 91,96% dan 8,04% dipengaruhi oleh variabel lain yang tidak diukur pada penelitian ini. Hasilevaluasi dan validasi prediksi menghasilkan nilai akurasi yang baik dengan nilai RMSE sebesar 0,3477. Kata kunci: data mining, tanaman kopi, crisp-dm, multiple linier progression.
Distribution and Fate of Escherichia coli in Lake Michigan Following Contamination with Urban Stormwater and Combined Sewer Overflows

ABSTRACT Escherichia coli distribution and persistence in nearshore Lake Michigan were assessed following heavy rains and sanitary sewer overflow (SSO) and combined sewer overflow (CSO) events over a 5-year period, including an 18-day period following 25.4 cm of rainfall in which intensive studies were conducted following multiple CSO and SSO events. E. coli levels in the Milwaukee estuary and harbor following SSO and CSO events ranged from 10 =4 to nearly 105 CFU/100 mL, which were significantly higher (p ≤ 0.05) than levels following rainfall alone. Sites outside of the breakwall but within the contamination plume (e.g., within 2 km of the harbor) were an order of magnitude lower. Locations 2–5 km from the harbor ranged from below detection limits, of < 1 to 5 CFU/100 mL. E. coli levels corrected for dilution based on specific conductivity measurements were lower than what would be expected for loss due to dilution alone, suggesting a combination of die-off and dilution, were responsible for the rapid disappearance of these organisms outside of the harbor. E. coli and fecal coliforms measured concurrently demonstrated that fecal coliforms could be recovered longer than E. coli in the open waters of the lake. E. coli isolated directly from sewage treatment plant influent were found to have a marked increase in antibiotic resistance traits for ten antibiotics commonly used in the human population compared with isolates from two animal sources of fecal pollution. However, E. coli obtained from sewage impacted water (n = 2,513) and from stormwater impacted water (n = 1,465) collected the previous year when there were no sewage overflows, were found to have no significant difference (p < 0.05) in the frequency of resistance when comparing the two conditions. E. coli survival characteristics and population dynamics are most likely influenced by multiple factors in complex systems such as the watershed/estuarine/lake environments of the Great Lakes.
Suppressive Effects of the Extract from Sake Cake on Chemical Mutagen-Induced SOS Response in Salmonella typhimurium

The suppressive effects of the extract from sake cake on the SOS response of Salmonella typhimurium TA 1535/ pSK1002 induced by AF-2, 4NQO, Trp-P-1, Trp-P-2, IQ and MeIQx were investigated for the purpose of finding antimutagenic substances in sake cake. The water-extract and methanol-extract from sake cake strongly suppressed SOS response induced by IQ in the presence of S9 mix in a dose dependent manner. The molecular weight of a suppressive compound in the water-extract was less than 3000 in an analysis of gel filtration. Several suppressive compounds also seemed to exist in the methanol-extract from sake cake.
Influence of historical events and contemporary estuarine circulation on the genetic structure of the banded killifish (Fundulus diaphanus) in the St. Lawrence River (Quebec, Canada)

Eight microsatellite markers were used to examine the historical and contemporary factors influencing the distribution of genetic variation within and among populations of Fundulus diaphanus (Lesueur, 1817) in the St. Lawrence River. Our results show that the contemporary hydrodynamics of the river affect levels of diversity and differentiation in this species. Genetic diversity increased towards downstream sites and levelled off at the upstream end of the tidal section, in the vicinity of Lake St-Pierre outlet. Likewise, differentiation and isolation-by-distance were most pronounced in the upstream, strictly fluvial section of the river. Surprisingly, however, we did not detect any significant intershore differentiation in this riparian fish species. Historical influence was also clearly detected and transcended the contemporary pattern, as revealed by an extremely strong isolation-by-distance pattern along the entire river stretch. This pattern likely results from the historical, postglacial secondary c.
Spatial-temporal of fire foci in the state of Rio de Janeiro, Brazil

This study evaluated the space-time variability of fire foci via environmental satellites for the State of Rio de Janeiro (SRJ) based on statistical procedures. The fire foci in the period of 2000 to 2015 were obtained from the BDQueimadas fire database. Descriptive, exploratory, and multivariate statistical analyses were performed in the software environment R i386 version 3.2. 5. The north region had 6760 foci (21.11%), the south-central region had 3020 foci (9.43%), the Middle Paraíba had 6,352 foci (19.84%), the Metropolitan areas had 6671 foci (20.83%), and the Green Coast region had 292 foci (0.91%). The cluster analysis identified three homogeneous groups of fire foci (G1, G2, and G3) but did not include the municipality of Campos dos Goytacazes (NA). The G1 group (6.21 ± 0.01 foci, 57.61%) included areas throughout the state and covered the coastal region and lowlands towards the north. The G2 group (6.21 ± 0.01 foci, 34.81%) included the northern, south-central, and coastal shallows regions. The G3 group (6.21 ± 0.01 foci, 9.78%) included the mountain ranges of the state. Environmental characteristics and socioeconomic are crucial in the dynamics of fire foci in Rio de Janeiro.
The Assessment of Infrasound and Low Frequency Noise Impact on the Results of Learning in Primary School – Case Study

The aim of the research was to determine the occurrence of possible, significant levels of infrasound and low frequency noise both in classrooms and around the primary school. Two sources of noise during research were significant: traffic on the national road and a wind farm, located near the school building. So far, few studies have been published regarding the impact of low-frequency, environmental noise from communication routes. The identification of hazards in a form of estimated noise levels resulted in preliminary information whether the location of the school near the road with significant traffic and the nearby wind farm can cause nuisance to children. There have been determined the criteria for assessing infrasound and low frequency noise. There have been made third octave band analyses of noise spectrum and the essential noise indicators were calculated. The results of learning in that school were thoroughly analysed for a long period of time and they were compared to the results obtained in other schools within a radius of 200 km situated near similar noise sources. Chosen assessment criteria show small exposure to low frequency noise. Measured infrasound noise levels are below hearing threshold.
Restriction of the upper distribution of New England cobble beach plants by wave‐related disturbance

1 The New England cobble beach plant community is an intertidal assemblage of halophytic forbs found exclusively behind fringing beds of the grass Spartina alterniflora. The purpose of this study was to determine the life stage and factors that limit the upper (landward) distribution of cobble beach plants in Narragansett Bay, Rhode Island, USA.
Theoretical Implications for the Evolution of Postsegregational Killing by Bacterial Plasmids

Some plasmids have been found to carry genes that kill their bacterial hosts after loss of the plasmid by vegetative segregation. This behavior appears at first to be purely spiteful because it does not benefit the plasmid in terms of reducing the competitive pressure from other plasmids. The conditions for establishment and maintenance of these killer plasmids are explored with a mathematical model of the dynamics of plasmid and bacterial populations maintained in continuous (chemostat) culture. This model demonstrates that when postsegregational killing leads to the killing of competing plasmids as well as hosts, the plasmid may derive a selfish advantage. This occurs when killing is coupled with infectious plasmid transfer, but the conditions are restrictive. The plasmid must have a high initial density, transfer at a high rate into cells carrying the competing plasmid, and be incompatible with the competing plasmid. The implications of these results and limitations of the model are discussed. The possibility that postsegregational killing is not the unique function maintaining these genes is raised.
BGSGA: Combining Bi-GRU and Syntactic Graph Attention for Improving Distant Supervision Relation Extraction

Distant supervision Relation Extraction(RE) aligns entities in a Knowledge Base (KB) with text to automatically construct large-labeled corpus, which alleviates the need for manual annotation in traditional RE. However, most existing models can't take full advantage of the syntactic structure information of each word in the dependency tree. In this paper, we propose BGSGA, a novel distant supervision RE model, to capture both semantic information and syntactic structure in the bag. BGSGA constructs the syntactic graph by combining the dependency trees of the sentences in the bag and then employs a syntactic structure attention mechanism to update the word embedding obtained from Bi-GRU. The syntactic structure attention mechanism captures the cross-sentence information with different weight by implementing the special attention operation between the target word and its neighbors of first-order and second-order in the graph. The experiments show that BGSGA outperforms our baseline models on benchmark datasets.
Nitrogen fertilization associated with cold storage and its impacts on the maintenance of peach quality

Nitrogen, which is considered the most important nutrient for peach trees, may interfere in both quantitative production characteristics and quality of fruits. This study aimed at evaluating the effect of the combination of doses of N fertilization and different periods of cold storage on physico-chemical and phytochemical characteristics of peaches in post-harvest. The experiment had a randomized complete block design in a 4x3 factorial scheme, i. e. , four doses of fertilization (0, 60, 120 and 180 Kg N ha-1) and three periods of storage (on the harvest day, on both the 15th and the 30th storage days at 1±1ºC, each followed by a day of simulated commercialization at 20±1ºC). The following aspects were evaluated in fruits yielded by peach trees of the genotype Cascata 1067: fruit color, soluble solid content, titratable acidity, pulp firmness, mass loss, total phenolic compounds and antioxidant activity. Different periods of cold storage and doses of N fertilization were found to affect epidermis luminosity, pulp firmness and titratable acidity of fruits. Peaches may be stored at low temperatures for 15+1 days. After that, loss of fruit firmness increases. N fertilization affects neither the soluble solid content nor the epidermis color of peaches, but both parameters are influenced by storage. Values of phenolic compounds and antioxidant activity decrease when there is N increment in the soil and when longer storage is carried out. Results suggest that peach composition may be affected by cultural practices, such as N fertilization, in harvest and after storage.
Note: Behaviour of Escherichia Coli O157: H7, Listeria Monocytogenes 4b and Yersinia Enterocolitica O3 in Pasteurised and Non-pasteurised Kefir Fermented for One or Two Days

The behaviour of three selected food-borne pathogens, E. coli O157: H7, L. monocytogenes 4b and Y. enterocolitica O3, added to fermented and pasteurised kefir was monitored. Populations of the three strains increased in one-day-fermented kefir, but only E. coli O157: H7 increased in two-days-fermented kefir during fermentation. None of the strains grew during cold storage (4 1 C), although E. coli O157: H7 and L. monocytogenes 4b survived up to 21 days in all samples cold. Y. enterocolitica O3 was the most susceptible strain that was present in one-day-fermented kefir for at least 14 days. Twodaysfermented kefir samples were more acidic thanthose of one-day-fermented samples, but none of the samples was safe enough to create an environment to eliminate the pathogens.
Molecular Cloning, Expression and Characterization of Human Peroxisome Proliferator Activated Receptors γ1 and γ2

Abstract We describe the molecular cloning and expression of cDNAs encoding human PPARγ1 and PPARγ2. Our sequences are distinct from the published sequence at 3 positions, resulting in nonconservative amino acid substitutions. In humans, PPARγ mRNA is expressed in spleen, bone marrow, liver, testis, skeletal muscle and brain, in addition to fat. Three thiazolidinediones were found to 1) displace a radiolabeled thiazolidinedione from both receptors with essentially the same IC 50 s and 2) to transactivate both PPARγ isoforms with similar EC 50 s in transient cotransfection assays utilizing the adipocyte-specific aP2 promoter. Saturating concentrations of these 3 thiazolidinediones altered the conformation of in vitro synthesized PPARγ protein producing a 27 kDa protease-resistant fragment. These results indicate that the antidiabetic effects of thiazolidinediones in humans are likely to be mediated via binding to and transactivation of PPARγ1 and γ2.