Miracle-dz/UniFAR-FTUs huggingface dataset:
Proteomic Evaluation of the Natural History of the Acute Radiation Syndrome of the Gastrointestinal Tract in a Non-human Primate Model of Partial-body Irradiation with Minimal Bone Marrow Sparing Includes Dysregulation of the Retinoid Pathway

Supplemental digital content is available in the text. Abstract Exposure to ionizing radiation results in injuries of the hematopoietic, gastrointestinal, and respiratory systems, which are the leading causes responsible for morbidity and mortality. Gastrointestinal injury occurs as an acute radiation syndrome. To help inform on the natural history of the radiation-induced injury of the partial body irradiation model, we quantitatively profiled the proteome of jejunum from non-human primates following 12 Gy partial body irradiation with 2.5% bone marrow sparing over a time period of 3 wk. Jejunum was analyzed by liquid chromatography-tandem mass spectrometry, and pathway and gene ontology analysis were performed. A total of 3,245 unique proteins were quantified out of more than 3,700 proteins identified in this study. Also a total of 289 proteins of the quantified proteins showed significant and consistent responses across at least three time points post-irradiation, of which 263 proteins showed strong upregulations while 26 proteins showed downregulations. Bioinformatic analysis suggests significant pathway and upstream regulator perturbations post-high dose irradiation and shed light on underlying mechanisms of radiation damage. Canonical pathways altered by radiation included GP6 signaling pathway, acute phase response signaling, LXR/RXR activation, and intrinsic prothrombin activation pathway. Additionally, we observed dysregulation of proteins of the retinoid pathway and retinoic acid, an active metabolite of vitamin A, as quantified by liquid chromatography-tandem mass spectrometry. Correlation of changes in protein abundance with a well-characterized histological endpoint, corrected crypt number, was used to evaluate biomarker potential. These data further define the natural history of the gastrointestinal acute radiation syndrome in a non-human primate model of partial body irradiation with minimal bone marrow sparing.
Cartan Geometry and Spin Networks

Spin network quantisation is a fundamental building block of loop quantum gravity, a backgroundindependent approach to nd a quantum theory of gravity. Here, we explore the possibility of applying this quantisation method to Cartan connections, which play a key role in an alternative formulation of gravity due to MacDowell and Mansouri. In this way, we seek to relate these two approaches. In order to make all of the mathematical tools accessible to non-experts, a self-contained introduction to principal connections, Cartan connections and their geometrical meaning is given.
Carrier and Transition Metals (M = Nb, Cr and Fe) Doping Effects on Structure and Electronic Structure in Spinel Li4Ti5O12 Compounds

Spinel Li4Ti5O12 (LTO) is one of the most promising candidate anode material for Li-ion battery (LIB) known, as zero strain material, it has poor intrinsic electronic properties. In order to enhance it, we have investigated effect of doping on electronic conductivity of spinel LTO phase structure. We consider the carrier and transition metal doping effect on structure and electronic structure of spinel LTO. It is shown that the doping can improve the electronic conduction of spinel LTO. Our calculations were based on the projector augmented wave (PAW) method with the generalized gradient approximation (GGA+U+J0) including the Hubbard U parameter for exchange correlation functional within the framework of density functional theory (DFT).
Comparative Adsorption Isotherm for Beryllium Oxide/Iron (III) Oxide Toward CO2 Adsorption and Desorption Studies

Surface modification of Fe2O3 by adding BeO was synthesized and calcined at different temperatures of 200-600 °C. The adsorbents were characterized by using XRD, N2 adsorption-desorption isotherm prior to performing CO2 adsorption and desorption studies. The CO2 adsorption data were analyzed using adsorption isotherm models such as Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich. BeO/Fe2O3-300 that calcined at 300 °C showed the most efficient adsorbent with physisorption and chemisorption were measured at 5.85 and 45.88 mg/g respectively. The CO2 adsorption notably best fitted with Freundlich isotherm with R2 = 0.9897 and calculated adsorption capacity closest to experimental data. This implies the CO2 adsorption process was governed by multilayer adsorption on the heterogeneous surface of the adsorbent. The mean free energy of adsorption (E=3.536 kJ/mol) from Dubinin-Radushkevich and heat of adsorption (bT=3.219 kJ/mol) from the Temkin model support that the adsorption process is physical phenomena.
Acute Hyperinsulinemia Alters Bone Turnover in Women and Men With Type 1 Diabetes

Type 1 diabetes (T1D) increases fracture risk across the lifespan. The low bone turnover associated with T1D is thought to be related to glycemic control, but it is unclear whether peripheral hyperinsulinemia due to dependence on exogenous insulin has an independent effect on suppressing bone turnover. The purpose of this study was to test the bone turnover marker (BTM) response to acute hyperinsulinemia. Fifty‐eight adults aged 18 to 65 years with T1D over 2 years were enrolled at seven T1D Exchange Clinic Network sites. Participants had T1D diagnosis between age 6 months to 45 years. Participants were stratified based on their residual endogenous insulin secretion measured as peak C‐peptide response to a mixed meal tolerance test. BTMs (CTX, P1NP, sclerostin [SCL], osteonectin [ON], alkaline phosphatase [ALP], osteocalcin [OCN], osteoprotegerin [OPG], osteopontin [OPN], and IGF‐1) were assessed before and at the end of a 2‐hour hyperinsulinemic‐euglycemic clamp (HEC). Baseline ON (r = −0.30, p = .022) and OCN (r = −0.41, p = .002) were negatively correlated with age at T1D diagnosis, but baseline BTMs were not associated with HbA1c. During the HEC, P1NP decreased significantly (−14.5 ± 44.3%; p = .020) from baseline. OCN, ON, and IGF‐1 all significantly increased (16.0 ± 13.1%, 29.7 ± 31.7%, 34.1 ± 71.2%, respectively; all p < .001) during the clamp. The increase in SCL was not significant (7.3 ± 32.9%, p = .098), but the decrease in CTX (−12.4 ± 48.9, p = .058) neared significance. ALP and OPG were not changed from baseline (p = .23 and p = .77, respectively). Baseline ON and SCL were higher in men, but OPG was higher in women (all p ≤ .029). SCL was the only BTM that changed differently in women than men. There were no differences in baseline BTMs or change in BTMs between C‐peptide groups. Exogenous hyperinsulinemia acutely alters bone turnover, suggesting a need to determine whether strategies to promote healthy remodeling may protect bone quality in T1D. © 2020 American Society for Bone and Mineral Research © 2020 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.
Study of Structural and Magnetic Properties of Spinel Zn Doped Cobalt Ferrites

Herein, the spinel Co1-xZnxFe2O4 (x = 0.0, 0.2, 0.4 and 0.6) powder samples have been prepared by the solid-state reaction method. We have carried out the measurements of crystal structure, element analysis, material characterization, magnetic property and Curie temperature using the X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS) and vibrating sample magnetometer, and the first-principles calculations within the framework of the density functional theory (DFT). The EDS measurement indicates that the Co1-xZnxFe2O4 powder samples have been successfully synthesized and exhibited the cubic spinel structures. Both the lattice constant and crystallite size increase with the Zn concentration due to the larger ionic radius of Zn2+ ion than the Co2+ ion. The concentration ratio of the Co2+ and Co3+ ions can be predicted by the distribution of cations between the A and B sites by the XPS measurement. For the magnetic properties, the residual magnetization, coercivity and Curie temperature decrease monotonically as the Zn concentration increases, while the saturation magnetization initially increases and then decreases at the room temperature. For the Co0.8Zn0.2Fe2O4 sample, the magnetic saturation reaches the maximum value of 62.98 Am2kg-1, due to a large amount of the Co3+ ions. The adequate replacement of Zn ion for the Co site can improve the magnetic properties of spinel Co1-xZnxFe2O4 powders, and effectively regulates the Curie temperature.
Study on Design and Combined Adaptive Control for a Remotely Operated Vehicle (VIAM-ROV900)

This paper presents the research of model-base design and control of Remotely Operated Vehicle (ROV) built in VietNam Automation & Mechatronics Laboratory (VIAMLAB). This is one of the most important types of underwater robots used in water environments for many purposes, especially for navy and marine industries. The design keeps our tethered ROV self-stabilized in the horizontal plane. It is also equipped with thrusters and sensor feedbacks, allowing 6 degrees-of-freedom motion. Moreover, cameras and grabber integrated into ROV support underwater survey tasks. In addition, the paper also simulates controllers with the main task of keeping depth for ROV. The controllers designed and surveyed here include: PID, optimal control (LQR), standard model control (MRAC) and combination controller between LQR and MRAC. The performance of the algorithm will be evaluated through simulation results using Matlab / Simulink.
Sexual dimorphism in the contribution of neuroendocrine stress axes to oxaliplatin-induced painful peripheral neuropathy.

While clinical studies support the suggestion that stress is a risk factor for painful chemotherapy-induced peripheral neuropathy (CIPN), there is little scientific validation to support this link. Here, we evaluated the impact of stress on CIPN induced by oxaliplatin, and its underlying mechanisms, in male and female rats. A single dose of oxaliplatin produced mechanical hyperalgesia of similar magnitude in both sexes, still present at similar magnitude in both sexes, on day 28. Adrenalectomy mitigated oxaliplatin-induced hyperalgesia, in both sexes. To confirm the role of neuroendocrine stress axes in CIPN, intrathecal administration of antisense oligodeoxynucleotide (AS-ODN) targeting β2-adrenergic receptor mRNA both prevented and reversed oxaliplatin-induced hyperalgesia, only in males. In contrast, glucocorticoid receptor AS-ODN, prevented and reversed oxaliplatin-induced hyperalgesia in both sexes. Unpredictable sound stress enhanced CIPN, in both sexes. The administration of stress hormones, epinephrine, corticosterone and their combination, at stress levels, mimicked the effects of sound stress on CIPN, in males. In females, only corticosterone mimicked the effect of sound stress. Also a risk factor for CIPN, early life stress, was evaluated by producing both stress-sensitive (produced by neonatal limited bedding, NLB) and stress-resilient (produced by neonatal handling, NH) phenotypes in adults. While NLB significantly enhanced CIPN only in female adults, NH significantly attenuated CIPN, in both sexes. Our study demonstrates a sexually dimorphic role of the two major neuroendocrine stress axes in oxaliplatin-induced neuropathic pain.
Adult lifetime reproductive value in fish depends on size and fecundity type

In a stable population, the adult lifetime reproductive value must be balanced against early life survival. Although delaying maturity may increase fecundity, it also reduces survival. Larger size at maturity therefore not only allows for higher fecundity, but requires it. Using simple arguments from life history, we derive a direct proportionality relationship between the adult lifetime reproductive value and weight at maturation and find that this relationship is consistent with empirical evidence from 28 stocks and species of bony fish from temperate–boreal environments. However, the expected proportionality falls off if mortality increases to include fishing. Furthermore, we find that the fecundity type (determinate or indeterminate) affects the predicted adult reproductive value, which is significantly (10-fold) higher for an indeterminate spawner than for a determinate spawner of the same weight. These differences may relate to trade-offs in the adult life history traits and (or) to seasonality in t.
Evaluation of the interaction of levothyroxine with bovine serum albumin using spectroscopic and molecular docking studies

Abstract Bovine serum albumin (BSA) acts as a carrier for many endogenous and exogenous compounds, such as thyroid hormones or corresponding drugs. Binding of the hydrophilic levothyroxine drug (LT4) to BSA is of significant pharmacological importance. In this work, UV-vis measurements were used to determine the pH value at which LT4 interacts optimally with proteins. The binding mechanism and affinity of the interaction between LT4 and BSA were investigated using Fourier-transform infrared spectroscopy (FT-IR), fluorescence, fluorescence resonance energy transfer (FRET), Surface Plasmon Resonance (SPR), supplemented by molecular docking analysis. Fluorescence measurements revealed the quenching effect of LT4 on the BSA intrinsic fluorescence and LT4 binding with BSA is driven by a ground-state complex formation that may be accompanied by a nonradiative energy transfer process. The thermodynamic parameters correspond to an enthalpic process, driven mainly by hydrogen bonds and van der Waals forces. Using SPR, the adsorbed amount of biomolecules was calculated and the binding affinity of LT4 with confined-BSA was characterized, indicating that the BSA immobilization plays an important role in LT4 binding. Docking studies confirmed the formation of the LT4-BSA complex with LT4 bound to site I on the BSA structure mainly with amino acid residues Trp 213, Tyr 137, Tyr 147. The calculation of the apparent association constant confirms the result obtained in SPR. Communicated by Ramaswamy H. Sarma
Use and impact of point-of-care ultrasonography in general practice: a prospective observational study

Objectives To describe how general practitioners (GPs) use point-of-care ultrasonography (POCUS) and how it influences the diagnostic process and treatment of patients. Design Prospective observational study using an online questionnaire before and after POCUS. Setting Office-based general practice. Participants Twenty GPs consecutively recruited all patients examined with POCUS in 1 month. Primary and secondary outcome measures We estimated the use of POCUS through the indication for use, the frequency of use, the time consumption, the extent of modification of the examination and the findings. The influence on the diagnostic process was estimated through change in the tentative diagnoses, change in confidence, the ability to produce ultrasound images and the relationship between confidence and organs scanned or tentative diagnoses. The influence of POCUS on patient treatment was estimated through change in plan for the patient, change in patient’s treatment and the relationship between such changes and certain findings. Results The GPs included 574 patients in the study. POCUS was used in patient consultations with a median frequency of 8.6% (IQR: 4.9–12.6). Many different organs were scanned covering more than 100 different tentative diagnoses. The median time taken to perform POCUS was 5 min (IQR: 3–8). Across applications and GPs, POCUS entailed a change in diagnoses in 49.4% of patients; increased confidence in a diagnosis in 89.2% of patients; a change in the management plan for 50.9% of patients including an absolute reduction in intended referrals to secondary care from 49.2% to 25.6%; and a change in treatment for 26.5% of patients. Conclusions The clinical utilisation of POCUS was highly variable among the GPs included in this study in terms of the indication for performing POCUS, examined scanning modalities and frequency of use. Overall, using POCUS altered the GPs’ diagnostic process and clinical decision-making in nearly three out of four consultations. Trial registration number NCT03375333.
The effects of ions on the secretion of extracellular proteases produced by cold-adapted deep-sea bacteriumPseudoalteromonas sp. SM9913

The deep-sea bacterium Pseudoalteromonas sp.SM9913 can secret abundant extracellular proteases when cultured in sea water ferment culture medium(swfcm). The effects of different ions on the extracellular protease secretion were studied.It is shown that the protease activity increases while the concentration of NaCl increases from 1%(w/v) to 5%(w/v). Mg~(2+) inhibit the extracellular proteases secretion obviously.The effect of Fe~(3+) is not remarkable.The addition of K~+,Ca~(2+) and phosphate at the same time could improve the extracellular protease secretion.Sr~(2+),F~-and B~-could improve the extracellular protease secretion of P.sp.SM9913 when acting separately, which combined effects are lower than their separate effects on the protease secretion.The combination of the above ions is shown to be able to increase the extracelluar protease secretion.New light has been shed on the scheme of extracellular protease secretion of cold-adapted deep-sea bacterium P.sp.SM9913.
Past, present, and future nuisance flooding on the Charleston peninsula

A forecast of nuisance flooding of Charleston peninsula is presented, based on an analysis of tide records from Charleston Harbor, SC. The forecast was based on past trends in local sea level and tidal harmonics, including the 18.6-yr lunar nodal and annual cycles. The data document an exponential rise in mean sea level. Extrapolating to year 2060 shows that the sea-level trend already is equivalent to the RCP4.5 scenario and on track to exceed NOAA’s intermediate low sea-level rise scenario of 0.5 m this century. If the trend continues, MSL will have risen by 0.22 m in 50 yr at an annual rate of 0.5 cm/yr in 2069. Simulations to 2064–2068, based on an empirical relationship between the annual number of flood events, defined as a water level exceeding 1.17 m NAVD (North American Vertical Datum of 1988), and the annual sum of monthly mean high water (r2 = 0.84), predict annual flood events will rise to the 60 to 75 range. Application of the hourly tidal harmonics to the long-term sea-level trend provided estimates of total land area flooded and duration of flooding. Flood duration is expected to rise to 6.5% by 2046–2050 and 8.2% of time by 2064–2068. The area exposed to flooding will be 4.23 km2 in 2046–2050 and 4.46 km2 in 2064–2068, corresponding to about 20–21% of peninsular area on what was formerly marshland and creeks, filled in earlier centuries. Finally, the estimated cost of defending the city and a proposal for a climate tax are discussed.
CORR Synthesis: What Is the Evidence for Age-appropriate Alignment Goals in Surgery for Adult Spinal Deformity?

The transition to patient-specific care in orthopaedic surgery encompasses an overarching effort to customize treatment plans based on each patient’s genetics, biomarkers, and a unique signature defined by imaging and other diagnostic modalities. The goal is to maximize the ability to predict a priori which patients will benefit from surgery and to individualize procedures to the extent possible. Within the field of adult spinal deformity surgery, multiple classification algorithms exist to categorize global malalignment based on variations in spinal morphology, severity of deformity, and age. The initial breakthrough in our understanding of patterns of variation in sagittal alignment began with Pierre Roussouly’s work with Johanes Dimnet, a biomechanical engineer, 15 years ago (Written communication, P. Roussouly, MD, January 16, 2020). They proposed a geometric model consisting of two arcs of a circle, with the tangent on the apex. The lower limit was the sacral plateau and the upper limit was free at the point where lordosis transitions into kyphosis. In this model, the lower arc was equal to sacral slope, closely linking lordosis and sacral slope. By introducing the variation of sacral slope inherent to the normal population, it was possible to predict distinct classes of lordosis curvature by lengthening or shortening the lower arc. This supplied the basis for the 2005 seminal paper by Roussouly et al. [27]. In Roussouly’s words when contacted for this review, “It took probably more than 1 year between the geometrical model and its application for classification” (Written communication, P. Roussouly, MD, January 16, 2020). This simple yet novel mathematical model was the catalyst for the discovery process and the concept that there is no single paradigm of ideal global alignment.
Population structure of Acrotrichis xanthocera (Matthews) (Coleoptera: Ptiliidae) in the Klamath Ecoregion of northwestern California, inferred from mitochondrial DNA sequence variation

Population Structure of Acrotrichis xanthocera (Matthews) (Coleoptera: Ptiliidae) in the Klamath Ecoregion of Northwestern California, Inferred from Mitochondrial DNA Sequence Variation. (May 2004) Ryan Matthew Caesar, B.A., The University of Texas at Austin Chair of Advisory Committee: Dr. Anthony I. Cognato The Klamath-Siskiyou Ecoregion of northern California and southern Oregon has extremely high biodiversity, but conservation centers on the protection of habitat for the northern spotted owl. A network of late successional reserves has been established without consideration of potential for protecting overall biodiversity, including genetic diversity. Mitochondrial DNA sequences are used to examine the population structure of Acrotrichis xanthocera (Coleoptera: Ptiliidae) sampled from five late successional reserves within the Klamath-Siskiyou Ecoregion and five comparison sites from northern California. Measures of gene flow, phylogenetic analysis, and nested clade analysis are employed to infer historical demographic and phylogeographic processes. Results show that A. xanthocera populations have undergone past range expansion, but gene flow is currently limited. Individual late successional reserves do not adequately protect the genetic variation in this species. Although further research is needed, these results are likely to be congruent for other edaphic arthropod species. Improvement of the late successional reserve system is warranted for maximum protection of the genetic diversity of soil arthropod populations.
Accurate Measurement of Copper Overload in an Experimental Model of Wilson Disease by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Wilson disease is a rare inherited autosomal recessive disorder. As a consequence of genetic alterations in the ATP7B gene, copper begins to accumulate in the body, particularly in the liver and brain. Affected persons are prone to develop liver cancer and severe psychiatric and neurological symptoms. Clinically, the development of corneal Kayser-Fleischer rings and low ceruloplasmin concentrations (<20 mg/dL) are indicative of Wilson disease. However, the detection of elevated hepatic copper content (>250 µg/g dry weight) alone is still considered as the best but not exclusive diagnostic test for Wilson disease. Presently, specific copper stains (e.g., rhodanine) or indirect staining for copper-associated proteins (e.g., orcein) are widely used to histochemically visualize hepatic copper deposits. However, these procedures only detect lysosomal copper, while cytosolic copper is not detectable. Similarly, elemental analysis in scanning electron microscope with energy dispersive X-ray analysis (EDX) often leads to false negative results and inconsistencies. Here, we tested the diagnostic potential of laser ablation inductively-coupled mass spectrometry (LA-ICP-MS) that allows quantitative analysis of multiple elements. Comparative studies were performed in wild type and the Atp7b null mouse model. We propose LA-ICP-MS as a versatile and powerful method for the accurate determination of hepatic copper in people with Wilson disease with high spatial resolution.