Miracle-dz/UniFAR-FTUs huggingface dataset:
CyTOF Cytometry by Time-of-Flight CXCL10 C-X-C motif chemokine 10 eGFP Enhanced green fluorescent protein FISH Fluorescence in situ Hybridization GCL Ganglion cell layer GPNMB Glycoprotein non-metastatic b hCMEC/D3 Human cerebral microvessel endothelial cells/D3 hiPSCs Human-induced pluripotent stem

Bone marrow-derived cells (BMDCs) are able to colonize the central nervous system (CNS) at sites of damage. This ability makes BMDCs an ideal cellular vehicle for transferring therapeutic genes/molecules to the CNS. However, conditioning is required for bone marrow-derived myeloid cells to engraft in the brain, which so far has been achieved by total body irradiation (TBI) and by chemotherapy (e.g. busulfan treatment). Unfortunately, both regimens massively disturb the host’s hematopoietic compartment. Here, we established a conditioning protocol to target myeloid cells to sites of brain damage in mice using non-myeloablative focal head irradiation (HI). This treatment was associated with comparatively low inflammatory responses in the CNS despite cranial radiation doses which are identical to TBI, as revealed by gene expression analysis of cytokines/chemokines such as CCL2, CXCL10, TNF-α and CCL5. HI prior to bone marrow transplantation resulted in much lower levels of blood chimerism defined as the percentage of donor-derived cells in peripheral blood (< 5%) compared with TBI (> 95%) or busulfan treatment (>50%). Nevertheless, HI effectively recruited myeloid cells to the area of motoneuron degeneration in the brainstem within 7 days after facial nerve axotomy. In contrast, no donor-derived cells were detected in the lesioned facial nucleus of busulfan-treated animals up to 2 weeks after transplantation. Our findings suggest that myeloid cells can be targeted to sites of brain damage even in the presence of very low levels of peripheral blood chimerism. We established a novel non-myeloablative conditioning protocol with minimal disturbance of the host’s hematopoietic system for targeting BMDCs specifically to areas of pathology in the brain. Citation: Böttcher C, Fernández-Klett F, Gladow N, Rolfes S, Priller J (2013) Targeting Myeloid Cells to the Brain Using Non-Myeloablative Conditioning. PLoS ONE 8(11): e80260. doi:10.1371/journal.pone. 0080260 Editor: Marc Tjwa, University of Frankfurt University Hospital Frankfurt, Germany Received August 6, 2013; Accepted October 11, 2013; Published November 7, 2013 Copyright: © 2013 Böttcher et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by Deutsche Forschungsgemeinschaft grants FOR 1336 and TRR43. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. * E-mail: josef.priller@charite.de
Plasma Cathepsin L Level is Positively Associated with Arterial Stiffness in Geriatric Patients

Advanced aging prompted vascular aging. As the consequence of vascular aging, arterial stiffness constitutes a potential risk factor for increased cardiovascular mortality in the elderly patients. Its role in the development of Cardiovascular Diseases (CVD) has been more emphatically studied in the past years [1]. Indirect ways to analyze arterial stiffness have been widely studied, Pulse Wave Velocity (PWV) is considered gold standard to evaluate arterial stiffness and is an independent marker of the presence of atherosclerotic cardiovascular diseases. Brachial-ankle PWV (baPWV), an index combining elastic and muscular peripheral arterial stiffness, is a conventional and noninvasive screening method to identify patients at high risk of cardiovascular disease [2,3].
The limiting effects of path dependence towards organizational change in the vehicle body building industry

The goal of this study is to identify the limiting effects of path dependence on organizational change through environmental influences. Whereby, the focus of the environmental influences within the study is on two different industries, namely the automotive- and flat-glass & glazing industry. Results of this study describe the limiting effects of path dependence on organizational change through environmental influences. Besides the limiting effects, also the process of how to identify path dependence within SME’s is comprehensively described. As a result, the enterprise under investigation could implement changes towards their business plan to decrease or prevent the limiting effects of these dependencies. Next to this, the identified path dependencies could also occur for similar enterprises, therefore the study could identify possible path dependencies for the vehicle body building industry.
Atrazine mineralization by Stenotrophomonas maltophilia and Agrobacterium tumefaciens Egyptian soil isolates

Atrazine is a water pollutant that has serious effects to human being and living organisms, thus it should be eliminated for environmental safety. Among the various methods used for its removal, biodegradation gains attraction due to its cost-effectiveness and environmental friendly nature. For this purpose, soil samples having previous history of treatment with atrazine were collected from El-Beheira Governorate, Egypt. The efficiency of the isolates to remove and mineralize atrazine was tested. Two bacterial strains achieved high removal percentage (up to 80% for S3) of atrazine during the first 7 h. These two bacterial strains were identified as Stenotrophomonas maltophilia and Agrobacterium tumefaciens using molecular identification based on sequencing of 16S rRNA gene. All the 16S nucleotides sequences of bacteria were applied and conserved in GeneBank. Agrobacterium tumefaciens mineralizes higher percentage of atrazine at shorter time relative to Stenotrophomonas maltophilia. External carbon source plays a key role in atrazine mineralization.
Associations of Ubiquitin-Specific Protease Genes with Resilience and Social Anxiety in Healthy Youths

Objective : Dynamic proteolysis, through the ubiquitin-proteasome system, is an important molecular mechanism for the constant regulation of synaptic plasticity and stress responses in humans. In this study, we examined whether genetic variants in the ubiquitin-specific peptidase (USP) genes were associated with psychological traits of resilience and susceptibility to neuropsychiatric disorders for each gender. Methods : A total of 344 Korean healthy youths (190 males, 154 females) were included in the study. A genotyping of rs2241646 of USP2 and rs346006 of USP46 was performed. The Connor–Davidson Resilience Scale and Brief Fear of Negative Evaluation Scale were administered for measuring trait resilience and social anxiety, respectively. The genetic associations of the USP variants were tested using multiple analyses of covariance with psychological traits as dependent variables after controlling for age in each gender. Results : For USP2 rs2241646, women with the TT genotype showed significantly higher resilience and lower social anxiety, as compared to those carrying the C allele. There were no associations between USP46 rs346005 and the psychological traits in both genders. Conclusions : The present study showed a possible genetic association between the USP2 rs2241646 and stress resilience and trait anxiety in women. The findings suggest that ubiquitin-proteasome system may be related to the resilience and susceptibility to stress-related neuropsychiatric disorders such as anxiety disorders, possibly through the regulation of dynamic proteolysis responses to stress. (Anxiety and Mood 2019; 15(2):122-126)
Seasonal and interannual variability in population dynamics of planktic foraminifers off Puerto Rico (Caribbean Sea)

Abstract. The state of a population of planktic foraminifers at a certain time reflects multiple processes in the upper ocean, including environmental conditions to which the population was exposed during its growth, the age of the cohorts, and spatiotemporal patchiness. We carried out depth-stratified (0–60, 60–100 m) replicated sampling off Puerto Rico in autumn 2012, revisiting three stations previously sampled in autumn 1994 and spring 1995, in order to analyze seasonal and interannual variability of planktic foraminifers and the stable isotopic composition of their tests. The merged dataset from all three sampling campaigns allows us to assess short- and long-term changes in foraminiferal population dynamics and the spatial assemblage coherency along the shelf edge. All three sample series cover more than 2 weeks during either spring (1995) or autumn (1994, 2012) and include the time of the full moon when reproduction of some surface-dwelling planktic foraminifers has been postulated to take place. Our analyses indicate that interannual variability affected the faunal composition, and both autumn assemblages were characterized by oligotrophic tropical species, dominated by Trilobatus sacculifer and Globigerinoides ruber (white and pink variety). However, G. ruber (white) had a higher abundance in 1994 (37 %) than in 2012 (3.5 %), which may be partially due to increasing sea surface temperatures since the 1990s. Between 60 and 100 m water depth, a different faunal composition with a specific stable oxygen isotope signature provides evidence for the presence of the Subtropical Underwater at the sampling site. Measurements on T. sacculifer sampled in autumn 2012 revealed that test size, calcification and incidence of sac-like chambers continued to increase after full moon, and thus no relation to the synodic lunar reproduction cycle was recognized. During autumn 2012, outer bands of hurricane Sandy passed the Greater Antilles and likely affected the foraminifers. Lower standing stocks of living planktic foraminifers and lower stable carbon isotope values from individuals collected in the mixed layer likely indicate the response to increased rainfall and turbidity in the wake of the hurricane.
Helmsman, Set a Course : Using a Compass and RFID Tags for Indoor Localisation and Navigation

Localisation and navigation are still two of the most important issues in mobile robotics. In certain indoor application scenarios RFID (radio frequency identification)-based absolute localisation has been found to be especially successful in supporting navigation. In this paper we evaluate the feasibility of an RFID and compass based approach to robot localisation and navigation for indoor environments that are dominated by corridors. We describe our system and evaluate its performance in a small, but full-scale, test
Prognostic and Clinicopathological Significance of MUC Family Members in Colorectal Cancer: A Systematic Review and Meta-Analysis

Objective To assess the association between MUC expression levels in colorectal cancer (CRC) tissues and prognosis and investigate the associations between MUC expression levels and CRC clinicopathological characteristics. Methods The PubMed, Embase, Cochrane Library, and Web of Science databases were searched from inception through September 13, 2019, to identify studies investigating the association between MUC expression levels in CRC tissues and prognosis. Pooled hazard ratios (HRs) or odds ratio (ORs) with 95% confidence intervals (CIs) were used to evaluate associations between MUC expression levels and prognosis or clinicopathological characteristics, respectively. The heterogeneity between studies was assessed by the I2 values, whereas the likelihood of publication bias was assessed by Egger's linear regression and Begg's rank correlation test. Results Among 33 included studies (n = 6032 patients), there were no associations between combined MUC phenotype expression levels and overall survival (OS) or disease-free survival (DFS)/relapse-free survival (RFS) in patients with CRC. In subgroup analyses, the upregulated MUC1 expression (HR = 1.50; 95% CI, 1.29–1.74; P < 0.00001) was associated with poor OS. However, the upregulated MUC2 expression (HR = 0.64; 95% CI, 0.52–0.79; P < 0.00001) was associated with better OS. Furthermore, a high level of MUC1 expression (HR = 1.99; 95% CI, 0.99–3.99; P = 0.05) was associated with shorter DFS/RFS. However, patients with a low level of MUC2 tumors showed better DFS/RFS than patients with a high level of MUC2 tumors (HR = 0.71; 95% CI, 0.49–1.04; P = 0.08; P = 0.0.009, I2 = 67%) and MUC5AC expression (HR = 0.56; 95% CI, 0.38–0.82; P = 0.003) was associated with longer DFS/RFS. In addition, a high level of MUC1 expression was associated with CRC in the rectum, deeper invasion, lymph node metastasis, distant metastasis, advanced tumor stage, and lymphatic invasion. A high level of MUC2 expression had a protective effect. High secretion of MUC5AC is associated with colon cancer compared with rectal cancer. Conclusion The protein expression of MUC1 might be a poor biomarker in colorectal cancer and might play a role in tumor transformation and metastasis. However, the protein expression of MUC2 expression might have a protective effect. Furthermore, randomized controlled trials (RCTs) of large patients are needed to confirm the results.
Attitudes towards Whistleblowers, Organizational Commitment, Ethical Climate Principles, and Self-Effi cacy as Determinants of Fraud Disclosures

* Corresponding author, email address: ABSTRACT Whistle-blowing is one of pro-social action done by organization members to give directions, procedures, or policies that have corrective impact on the organizations. The brave action to disclose fraud is determined by internal and external factors of individuals. This research is conducted to test factors that infl uence people to conduct whistleblowing, such as attitudes towards whistleblowers, ethical climate-principles, organization commitment, and self-effi cacy. This research also has objective to test the applications of Theory of Planned Behavior (TPB) and Pro-social Organizational Behavior (POB) particularly related to fraud. The research methodology is conducted using questionaire and analyzed using Multiple Regression. Results of the research show that attitudes towards whistleblowers, ethical climate principles, organizational commitments, and self-effi cacy have a signifi cant effect on the intention to conduct whistle-blowing.
Energy saving scheduling in a fog-based IoT application by Bayesian taskclassification approach

The Internet of things increases information volume in computer networks and the concept of fog will help us to control this volume more efficiently. Scheduling resources in such an environment would be an NP-Hard problem. This article has studied the concept of scheduling in fog with Bayesian classification which could be applied to gain the task requirements like the processing ones. After classification, virtual machines will be created in accordance with the predicted requirements. The ifogsim simulator has been applied to study our fog-based Bayesian classification scheduling (FBCS) method performance in an EEG tractor application. Algorithms have been evaluated on a practical application of brain signal tracking system. According to the results, the FBCS method, compared with other methods, has reduced the energy consumption in the cloud and the executing task cost in cloud; and also the average of energy consuming in mobiles has been decreased by smart decision making.
AUDITING INTEGRITY – THROUGH CHALLENGE TO ADDED VALUE

The aim of this paper is to explain the essence and significance of integrity as an element of organizational culture (i.e. control/internal environment), but also the issues of auditing/assessment of integrity as ‚soft’ control. Since many academic and professional researches and case studies has shown that organizational integrity, or the lack of it, has multiple effects on the quality of overall corporate governance, including the integrity of financial reporting, internal control systems and risk management, it is not surprising that legislation and auditing standards have introduced the obligation of assessment and reporting on the control environment/organizational culture, including the ethics, integrity and tone at the top. Considering the mission of auditing profession (especially internal auditing), the first prerequisite in this context is to define ‚integrity’ because this definition represents the criterion for its effective assessment. However, the lack of general consensus on integrity definition is just one of many challenges auditors are facing. This paper shows that regular assessments of control environment and its elements, including the integrity, do contribute to its empowerment. Likewise, strong control environment, i.e. organizational culture, by itself, provides many different advantages resulting in overall business performance. This paper suggests that integrity carries real value, just like knowledge, technology, human, physical and social capital. Therefore, there are no formal controls that could replace or compensate for the lack of organizational integrity and auditing the integrity may create real added value.
Circulating tumor cells in lung cancer are prognostic and predictive for worse tumor response in both targeted- and chemotherapy.

Background It is unknown whether the presence of circulating tumor cells (CTC), a known prognostic factor, influences treatment outcome. We investigated whether baseline CTC in non-small cell lung cancer (NSCLC) patients treated with tyrosine kinase inhibitors (TKI) or chemotherapy was associated with response to therapy. Methods We included consecutive advanced NSCLC patients, stratified by therapy. Before treatment the number of CTC was measured by CellSearch. Tumor response rates, progression free survival (PFS) and overall survival (OS) in patients with and without CTC at baseline were compared. Results We included 86 patients (34 treated by TKI). Response rates of patients with CTC were lower than in patients without CTC (OR =0.22, P<0.01, adjusted for performance score and smoking status). In both treatment groups, the difference in response rates between patients with and without CTC was similar (TKI response: 25% with CTC versus 73% without CTC, chemotherapy response: 35% versus 51% respectively, interaction P=0.17). CTC was associated with a worse PFS [hazard ratio (HR) =2.0, 95% confidence interval (CI): 1.2-3.2, P=0.01] and OS (HR =1.7, 95% CI: 1.1-2.8, P=0.03) after adjustment for performance score and stage. The association remained significant after adding tumor response to the model (PFS: HR =1.9, 95% CI: 1.0-3.0, P=0.01, OS: HR =1.6, 95% CI: 1.0-2.6, P=0.05). No significant interaction between CTC presence and therapy was observed (P=0.42 for PFS and P=0.83 for OS). Conclusions Presence of CTC in advanced NSCLC patients is associated with low response rates, shorter PFS and OS, independent of the received therapy.
Multi-objective Optimization of a Solar Driven Combined Power and Refrigeration System Using Two Evolutionary Algorithms Based on Exergoeconomic Concept

This paper deals with a multi-objective optimization of a novel micro solar driven combined power and ejector refrigeration system (CPER). The system combines an organic Rankine cycle (ORC) with an ejector refrigeration cycle to generate electricity and cold capacity simultaneously. Major thermodynamic parameters, namely turbine inlet temperature, turbine inlet pressure, turbine back pressure, and evaporator temperature are selected as the decision variables. Three objective functions, namely the energetic efficiency, exergetic efficiency and cost rate of products are selected for optimization. NSGA-II and MOPSO are employed and compared, to achieve the final solutions in the multi-objective optimization of the system operating. It is found that the values of the energetic and exergetic efficiencies increase within 27.7% and 26.1%, respectively and the cost rate of products decreases by about 32.7% with respect to base case.
Depression, anxiety and stress among postgraduate students in Faculty of Education of a public university in Malaysia

Introduction: Psychological problems like anxiety, depression and stress significantly exacerbate the pressure on students to perform better. These factors collectively hamper their performance leading to low academic achievement especially among postgraduate students who majority of them are working and married. This study aims to identify the level of depression, anxiety and stress among postgraduate students, and to investigate the correlation between depression, anxiety and stress based on gender, marital status and age. Methods: The study was a quantitative survey and correlational research design using a clustered random sampling, selected from all postgraduate students (179) in Faculty of Education. The DASS-21 was used as measuring tools for depression, anxiety and stress among post-graduate students. Results: Findings of the study revealed that majority of the respondents are having moderate level of depression, anxiety and stress. Meanwhile, there were strong correlations between depression, anxiety and stress. However, there were no significant differences for level of depression, anxiety and stress based on demographic factors (gender, marital status and age). Conclusion: Based on the findings, it can be concluded that depression, anxiety and stress are strongly correlated. This study can give some insight to the educators, universities administration as well as students’ family to develop common intervention on how to curb depression, anxiety and stress among postgraduate students.