Miracle-dz/UniFAR-FTUs huggingface dataset:
Handgrip Strength Seems Not to Be Affected by COPD Disease Progression: A Longitudinal Cohort Study

Abstract Skeletal muscle dysfunction, functional exercise capacity impairment and reduced physical activity are characteristic features in patients with chronic obstructive pulmonary disease (COPD). Assessments addressing muscle strength of the upper limb, such as measurement of handgrip strength (HGS), are rarely performed and reported. We aimed to analyze the course of HGS and possible predictors of changes in HGS over time in COPD. Yearly assessments of various disease markers were performed for a follow-up of up to seven years in a cohort of COPD patients to assess the longitudinal disease process. Data of 194 patients with at least one follow-up measurement were analyzed. HGS decreased significantly by B = −0.86 (95% CI −1.09/−0.62, p < 0.001) over time. The multivariate mixed effects model showed an independent association between greater annual declines in HGS and lower numbers of steps per day by B = 0.11 (95% CI 0.03/0.18, p = 0.006) and an enhanced change in COPD Assessment Test scores by B = −0.01 (95% CI −0.01/−0.00, p = 0.034). No evidence for an independent association between annual decline in HGS and FEV1% pred. by B = −0.01 (95% CI −0.03/0.01, p = 0.297) was shown. Patients who died during follow-up did not exhibit greater declines in HGS compared to survivors (p = 0.884). Although HGS significantly decreased over time, no pathophysiological link with COPD disease progression could be demonstrated. Previous cross-sectional associations between HGS and mortality could not be confirmed in this longitudinal setting. Our data suggests that repeated monitoring of HGS in clinical settings seems not to be helpful to predict COPD specific disease progression.
Accelerated landing in a stingless bee and its unexpected benefits for traffic congestion

To land, flying animals must simultaneously reduce speed and control their path to the target. While the control of approach speed has been studied in many different animals, little is known about the effect of target size on landing, particularly for small targets that require precise trajectory control. To begin to explore this, we recorded the stingless bees Scaptotrigona depilis landing on their natural hive entrance—a narrow wax tube built by the bees themselves. Rather than decelerating before touchdown as most animals do, S. depilis accelerates in preparation for its high precision landings on the narrow tube of wax. A simulation of traffic at the hive suggests that this counterintuitive landing strategy could confer a collective advantage to the colony by minimizing the risk of mid-air collisions and thus of traffic congestion. If the simulated size of the hive entrance increases and if traffic intensity decreases relative to the measured real-world values, ‘accelerated landing' ceases to provide a clear benefit, suggesting that it is only a useful strategy when target cross-section is small and landing traffic is high. We discuss this strategy in the context of S. depilis' ecology and propose that it is an adaptive behaviour that benefits foraging and nest defence.
Providing Information-Seeking Skills Feedback Within a Medical School Curriculum: A Partnership between Librarians and Education Specialists

Abstract The Liaison Committee on Medical Education (LCME) revised their standards for MD program curricula with increased emphasis on the teaching and assessment of skills needed for life-long learning. This case study describes the collaboration between a team of librarians and a curriculum office to continuously improve information-seeking skills activities for medical students. This positive collaborative experience has firmly embedded librarians into the medical curriculum. Based on student feedback, the majority of students found the librarian-involved activities useful.
Outcomes in Anomalous Aortic Origin of a Coronary Artery Following a Prospective Standardized Approach

Supplemental Digital Content is available in the text. Background: Anomalous aortic origin of a coronary artery (CA) is the second leading cause of sudden cardiac death in young athletes. Management is controversial and longitudinal follow-up data are sparse. We aim to evaluate outcomes in a prospective study of anomalous aortic origin of CA patients following a standardized algorithm. Methods: Patients with anomalous aortic origin of a CA were followed prospectively from December 2012 to April 2017. All patients were evaluated following a standardized algorithm, and data were reviewed by a dedicated multidisciplinary team. Assessment of myocardial perfusion was performed using stress imaging. High-risk patients (high-risk anatomy—anomalous left CA from the opposite sinus, presence of intramurality, abnormal ostium—and symptoms or evidence of myocardial ischemia) were offered surgery or exercise restriction (if deemed high risk for surgical intervention). Univariate and multivariable analyses were used to determine predictors of high risk. Results: Of 201 patients evaluated, 163 met inclusion criteria: 116 anomalous right CA (71%), 25 anomalous left CA (15%), 17 single CA (10%), and 5 anomalous circumflex CA (3%). Patients presented as an incidental finding (n=80, 49%), with exertional (n=31, 21%) and nonexertional (n=32, 20%) symptoms and following sudden cardiac arrest/shock (n=5, 3%). Eighty-two patients (50.3%) were considered high risk. Predictors of high risk were older age at diagnosis, black race, intramural course, and exertional syncope. Most patients (82%) are allowed unrestrictive sports activities. Forty-seven patients had surgery (11 anomalous left CA and 36 anomalous right CA), 3 (6.4%) remained restricted from sports activities. All patients are alive at a median follow-up of 1.6 (interquartile range, 0.7–2.8) years. Conclusions: In this prospective cohort of patients with anomalous aortic origin of a CA, most have remained free of exercise restrictions. Development of a multidisciplinary team has allowed a consistent approach and may have implications in risk stratification and long-term prognosis.
Accuracy of Digital Impressions in Fixed Prosthodontics: A Systematic Review of Clinical Studies.

PURPOSE To determine the accuracy of direct digital impressions in vivo and compare it to that of conventional impressions in order to assess whether intraoral scanners could be a legitimate alternative for the manufacturing of fixed prosthodontics. MATERIALS AND METHODS An electronic systematic search was conducted in the PubMed, Web of Science, Embase, and Cochrane Library databases using query terms such as digital impression; intraoral digital impression; intraoral scanner; intraoral digital scanner; conventional impression; analogue impression; and accuracy. The outcomes were the trueness and precision of digital impressions in comparison to those of high-precision conventional impressions in vivo. RESULTS Six studies matched the inclusion criteria. Four studies investigated the precision of digital impressions, while two studies focused on their trueness. Even if all the authors agreed that conventional impressions were more accurate than digital impressions in vivo, they gave different opinions on using intraoral scanners in a clinical context without causing misfit of the final restoration. CONCLUSION According to the results of the present review, conventional impressions performed using high-precision impression materials showed greater accuracy than digital impressions. However, additional studies assessing the accuracy of digital impressions in a wider variety of clinical situations are highly recommended.
Under-Etched Plasmonic Disks on Indium Tin Oxide for Enhanced Refractive Index Sensing on a Combined Electrochemical and Optical Platform

A simple approach to enhance the refractive index sensitivity of gold nanodisks immobilized on electrically conducting indium tin oxide (ITO) substrates has been demonstrated. A two-fold increase in sensitivity to bulk refractive index change was achieved by substrate under-etching of gold nanodisks on ITO in 50 mM sulfuric acid. The influence of an intermediate titanium adhesion layer was investigated and was found to markedly influence the etching pattern and time. Etching with an adhesion layer resulted in enhanced refractive index sensitivity on disk-on-pin like structures after long etching times, whereas etching of disks deposited directly on ITO resulted in a disk-on-pincushion like configuration and similarly enhanced sensitivity already at shorter times. The gold disks remained electrically connected to the ITO substrate throughout etching and allowed site-specific electrodeposition of poly(3-aminophenol) at the nanodisks, showing enhanced thin-film refractive index sensitivity. This work demonstrates a simple method for enhancing refractive index sensitivity of nanostructures on ITO substrates for combined electrochemical and optical platforms, and subsequently a method to modify the surface of the electrically connected nanostructures, which has potential application in biosensing.
Deep Learning-driven research for drug discovery: Tackling Malaria

Malaria is an infectious disease that affects over 216 million people worldwide, killing over 445,000 patients annually. Due to the constant emergence of parasitic resistance to the current antimalarial drugs, the discovery of new drug candidates is a major global health priority. Aiming to make the drug discovery processes faster and less expensive, we developed binary and continuous Quantitative Structure-Activity Relationships (QSAR) models implementing deep learning for predicting antiplasmodial activity and cytotoxicity of untested compounds. Then, we applied the best models for a virtual screening of a large database of chemical compounds. The top computational predictions were evaluated experimentally against asexual blood stages of both sensitive and multi-drug-resistant Plasmodium falciparum strains. Among them, two compounds, LabMol-149 and LabMol-152, showed potent antiplasmodial activity at low nanomolar concentrations (EC50 <500 nM) and low cytotoxicity in mammalian cells. Therefore, the computational approach employing deep learning developed here allowed us to discover two new families of potential next generation antimalarial agents, which are in compliance with the guidelines and criteria for antimalarial target candidates.
The Effects of Substituting Dietary Soybean Meal with Maize Grain on Milk Production in Dairy Goats

Simple Summary Today the environmental sustainability of livestock has become increasingly important. Nitrogen excretion from livestock can lead to air and groundwater pollution, causing acid rain, fine air particulates, and greenhouse gas emissions that lead to climate change, groundwater nitrate poisoning, and surface water eutrophication. Often, livestock are fed diets with a protein content that is much higher than what they require. In this experiment, a herd of dairy goats of the Alpine breed, reared on a farm in the hills near Genoa, Italy, was used to study the impacts of being fed a low- or a high-protein diet. The high-protein diet did not improve milk yield and quality; on the contrary, it lowered the efficiency of dietary-nitrogen utilization, increased feeding cost, and, above all, increased nitrogen excretion and the associated environmental risks. In conclusion, a high-protein diet is not advisable, both economically and environmentally, and a precision feeding approach (i.e., supplying the nutrients required for a given body mass and level of production, and no more) is highly recommended. Abstract In view of better environmental sustainability, livestock diets must not exceed protein requirements, as often happens with lactating goats reared in semi-intensive systems. The aim of this experiment was to verify in real-breeding conditions the influence of two diets with different protein contents (% crude protein (CP) on dry matter (DM)): 16.0 (high-protein diet; HP) vs 12.2 (low-protein diet; LP) on milk production in dairy goats. The diets differed only in the replacement—in the LP diet—of 250 g soybean meal with 250 g maize grain meal. Twenty-three Alpine goats were divided into two groups and used in a cross-over feeding trial for 2 months. Animals were weighed at the beginning of each month of the trial, and feed intake and milk yield and composition were recorded weekly. HP and LP did not differ statistically for milk yield and composition (3.32 vs 3.42 kg milk/d, 3.21% vs 3.27% fat, 3.31% vs 3.27% protein for HP and LP, respectively), but the HP diet determined a higher milk urea content (51.2 vs 36.6 mg/dL, p < 0.001) and a worse efficiency of nitrogen utilization (28.0% vs 37.2%). In conclusion, the LP diet resulted in a reduction of urinary nitrogen excretion by 28% and of the feed cost by about 10%.
Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy

Mutations in genes encoding synaptic proteins cause many neurodevelopmental disorders, with the majority affecting postsynaptic apparatuses and much fewer in presynaptic proteins. Syntaxin-binding protein 1 (STXBP1, also known as MUNC18-1) is an essential component of the presynaptic neurotransmitter release machinery. De novo heterozygous pathogenic variants in STXBP1 are among the most frequent causes of neurodevelopmental disorders including intellectual disabilities and epilepsies. These disorders, collectively referred to as STXBP1 encephalopathy, encompass a broad spectrum of neurologic and psychiatric features, but the pathogenesis remains elusive. Here we modeled STXBP1 encephalopathy in mice and found that Stxbp1 haploinsufficiency caused cognitive, psychiatric, and motor dysfunctions, as well as cortical hyperexcitability and seizures. Furthermore, Stxbp1 haploinsufficiency reduced cortical inhibitory neurotransmission via distinct mechanisms from parvalbumin-expressing and somatostatin-expressing interneurons. These results demonstrate that Stxbp1 haploinsufficient mice recapitulate cardinal features of STXBP1 encephalopathy and indicate that GABAergic synaptic dysfunction is likely a crucial contributor to disease pathogenesis.
Synthetic livestock vaccines as risky interference with nature? Lay and expert arguments and understandings of “naturalness”

The article describes how the idea of “naturalness” was used by three different groups in arguments over the risk of livestock vaccines developed in synthetic biology. Based on interviews with two groups of scientific experts and focus groups with lay people in five European countries, and using Toulmin’s argument analysis as the analytical tool, the article maps and compares the different ways in which “naturalness” was used as a warrant. Several notions of “naturalness” are involved in lay people’s reasoning and several lay people’s understandings of risk relied on their perceptions of the “unnaturalness” of the synthetic vaccines. The notion of “naturalness” was used less by synthetic biology experts and not at all by vaccine experts. Lay people see the vaccine as less natural than other vaccines and therefore as a greater risk. In contrast, synthetic biology experts understand synthetic biology as natural, and relate naturalness, unpredictability and risk.
Double hybrids and time‐dependent density functional theory: An implementation and benchmark on charge transfer excited states

In this paper we present the implementation and benchmarking of a Time Dependent Density Functional Theory approach in conjunction with Double Hybrid (DH) functionals. We focused on the analysis of their performance for through space charge‐transfer (CT) excitations which are well known to be very problematic for commonly used functionals, such as global hybrids.Two different families of functionals were compared, each of them containing pure, hybrid and double‐hybrid functionals.The results obtained show that, beside the robustness of the implementation, these functionals provide results with an accuracy comparable to that of adjusted range‐separated functionals, with the relevant difference that for DHs no parameter is tuned on specific compounds thus making them more appealing for a general use. Furthermore, the algorithm described and implemented is characterized by the same computational cost scaling as that of the ground state algorithm employed for MP2 and double hybrids.
Treatment of painful polyneuropathies of diabetic and other origins with 10 kHz SCS: a case series

ABSTRACT Painful diabetic polyneuropathy (PDPN) and painful polyneuropathies of other origins are associated with significant personal and societal burdens with treatments limited to symptomatic management. Treatment options include antidepressants, gamma-aminobutyric acid (GABA) analogs, opioids, and topical analgesics, which are intended to alleviate pain and symptoms of neuropathy, but limited data are available on their efficacy. Paresthesia-based low-frequency spinal cord stimulation (LF-SCS) is considered a last-resort treatment modality for PDPN patients. In a large-scale RCT of neuropathic low back and leg pain, high-frequency SCS at 10 kHz (10 kHz SCS) was shown to provide superior pain relief that is not dependent on paresthesia and a higher responder rate than LF SCS. This retrospective case series includes data from six patients with painful peripheral neuropathies, including PDPN, idiopathic polyneuropathy, and chronic inflammatory demyelinating polyneuropathy, who were candidates for 10 kHz SCS in routine commercial practice. All patients reported a reduction in verbal numerical rating scale (VNRS) pain score at last follow-up (2.7 ± 0.9) compared with baseline (7.0 ± 0.9). Out of five patients with information available at last follow-up, two were completely off their pain medications and two reduced their dose by over 40%. Similarly, at last follow-up, three out of five patients reported sensory improvement in their lower limbs. In conclusion, 10 kHz SCS treatment resulted in significant pain relief in all the patients, decreased reliance on pain medication, and improved lower limb sensory function in the majority of patients.
Usefulness of real-time PCR during follow-up of patients treated with Benznidazole for chronic Chagas disease: Experience in two referral centers in Barcelona

Background Antitrypanosomal treatment with Benznidazole (BZ) or Nifurtimox may be recommended for patients with chronic Chagas disease (CD) to reduce the onset or progression of symptoms. However, such treatment has limited efficacy and high level of toxic effects. In addition, the current cure biomarker (serology conversion) precludes any treatment assessment unless a prolonged follow-up is arranged. PCR is thus the most useful, alternative surrogate marker for evaluating responses to treatment. The aim of this study is to describe the usefulness of real-time PCR in monitoring BZ treatment within a large cohort of chronic CD cases in Barcelona. Methodology/Principal findings A total of 370 chronic CD patients were monitored with real-time PCR post-BZ treatment. The median follow-up was 4 years (IQR 2.2–5.3y), with a median of 3 clinical visits (IQR 2–4). Only 8 patients (2.2%) presented with at least one incident of positive real-time PCR after treatment and were therefore considered as treatment failure. Four of those failure patients had completed full course treatment, whereas the remaining cases had defaulted with a statistical difference between both groups (p = 0.02). Half of the failure patients had undergone less than 4 years of follow-up monitoring all presented with parasitemia before treatment. Conclusions/Significance BZ treatment failure was highly infrequent in our cohort. BZ discontinuation was a risk factor for positive real-time PCR results during clinical follow-up. Regular testing with real-time PCR during follow-up allows for early detection of treatment failure in patients with chronic CD.
Affectivity in schizophrenia: Its relations with functioning, quality of life, and social support satisfaction.

OBJECTIVE To evaluate the relationship between affectivity and sociodemographic and clinical characteristics, quality of life, functioning, and social support satisfaction in schizophrenia. METHOD Cross-sectional study of a sample of 282 schizophrenic patients. An interview was performed using a sociodemographic and clinical questionnaire and scales to evaluate positive and negative affect, quality of life, social support satisfaction, and functioning. RESULTS Participants' employment status, gender, whether they smoked, hospitalization in the last year, antidepressant medication and benzodiazepines, quality of life, functioning, and satisfaction with social support had relationships with positive or negative affect. CONCLUSION This study provides further understanding of the factors related to affectivity in schizophrenia. More studies are needed to prove these relations and their effects on rehabilitation in people with schizophrenia.
Increased monocyte and T-cell activation in treated HIV+ ugandan children: associations with gut alteration and HIV factors.

INTRODUCTION The pathophysiology of immune activation and its mechanisms in children living with perinatally acquired HIV (PHIV) in sub-Saharan Africa has been understudied. METHODS We enrolled 101 children living with perinatally acquired HIV (PHIV) and 96 HIV negative controls (HIV-). All participants were between 10-18 years of age with no known active infections. PHIVs were on ART with HIV-1 RNA level ≤400 copies/mL. We measured plasma and cellular markers of monocyte activation, T-cell activation (expression of CD38 and HLA-DR on CD4+ and CD8+), oxidized lipids, markers of gut integrity and fungal translocation. Spearman correlations and linear regression models were used. RESULTS Overall median (Q1; Q3) age was 13 years (11; 15) and 52% were females. Groups were similar by age, sex and BMI. Median ART duration was 10 years (8; 11). PHIVs had higher monocyte and T-cell activation; higher sCD14 (p = 0.01) and elevated frequencies of non-classical monocytes (p < 0.001 for both). Markers of systemic inflammation (hsCRP), fungal translocation (BDG), intestinal permeability (zonulin) and oxidized lipids (ox LDL) correlated with monocyte and T cell activation in PHIV (≤0.05). After adjusting for age, sex, ART duration, protease inhibitor and non-nucleoside reverse transcriptase inhibitor use, a modest association between BDG and activated CD4+ T cells was observed (β=0.65, p < 0.01). Oxidized LDL was inversely associated with activated T cells, inflammatory and non-classical monocytes (p < 0.01). CONCLUSION Ugandan children with perinatally acquired HIV with viral suppression have evidence of ongoing immune activation. Intestinal barrier dysfunction and fungal translocation may be involved in chronic immune dysfunction.