Miracle-dz/UniFAR-FTUs huggingface dataset:
Combination Effect of Diurnal Exposure to Sucrose and Nocturnal Exposure to Lactose on Enamel Demineralization

We have hypothesized that the association between human milk and caries in breastfeeding children could be explained by the combination of a diurnal cariogenic diet with the nocturnal lactose fermentation, conditions simulated in this experimental study. Cariogenic biofilm was formed on bovine enamel slabs, which were exposed 8x/day for 3 min to a 10% sucrose solution, simulating a highly cariogenic diurnal diet, or 50 mM NaCl solution (control). Simulating the nocturnal retention of milk in mouth, biofilms were transferred to culture medium containing 0.7% lactose for 2 h, or only to culture medium (control). Four groups were designed (n = 12): Ctrl, no exposure to diurnal sucrose or nocturnal lactose; Lac, only nocturnal exposure to lactose (2 h); Suc, only diurnal exposure to sucrose (8x/day); and Suc→Lac, diurnal exposure to sucrose (8x/day) followed by nocturnal exposure to lactose (2 h). The medium was changed 3x/day, at the beginning of the day and after diurnal and nocturnal exposures. Calcium in the medium was determined as a chemical indicator of partial demineralizations occurred during the diurnal and the nocturnal treatments; the medium pH was also determined. After 96 h of growth, biofilms were harvested to evaluate CFU, biomass, and extracellular polysaccharides, soluble and insoluble. The percentage of enamel surface hardness loss (%SHL) was evaluated as cumulative demineralization. Data were analyzed by one-way ANOVA and Tukey’s test (α = 5%). Highest %SHL (p < 0.05) was found for the Suc→Lac (40.6%) group when compared to Suc (32.1%), Lac (6.6%), and Ctrl (2.4%) groups. Calcium released during the diurnal and nocturnal treatments was, respectively, Suc→Lac = Suc > Lac = Ctrl and Suc→Lac > Lac > Suc = Ctrl (p < 0.05). Regarding the Ctrl group, calcium released from nocturnal lactose fermentation by the Suc→Lac group was 4-fold greater than that provoked by the Lac group. The findings were supported by the pH of the media. The data suggest that the biofilm formed under diurnal exposure to sucrose enhances the cariogenicity of nocturnal exposure to lactose.
Association of the Labor Migration of Parents With Nonsuicidal Self-injury and Suicidality Among Their Offspring in China

Key Points Question Is the labor migration of parents in China associated with nonsuicidal self-injury (NSSI) or suicidality among children, adolescents, and young adults who were left behind, and are there sex differences in any association? Findings In this cross-sectional study in China of 15 312 participants, labor migration of the father or of both parents was associated with 1 to 4 (but not ≥5) episodes of NSSI in 1 year and with suicidality among offspring, whereas differential associations by sex were minimal. In addition, higher odds of experiencing 1 to 4 episodes of NSSI were found for offspring who were initially separated from 1 or both parents at preschool age. Meaning These findings suggest that interventions to address NSSI should consider potential associations with parental migration.
Rectangular dielectric resonator antenna for ultrawideband applications

A new dielectric resonator antenna (DRA) is introduced for ultrawideband applications. A rectangular dielectric resonator, a bevel feeding patch and an airgap between the DR and ground plane are used to obtain an ultrawideband impedance bandwidth. The effective dielectric constant and the Q-factor can be reduced by using the airgap and the bevel-shaped feeding mechanism, which can provide a smooth transition from one resonant mode to another. Measured results demonstrate that the proposed DRA has a wide bandwidth from 2.6 to 11 GHz with VSWR less than two, covering the frequency range of more than 120 . Experimental and numerical results are carried out and discussed, showing good agreement.
Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019

ABSTRACT Objectives: Since January 2019, the Belgian National Reference Center for Mycobacteria (NRC) has switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted Mycobacterium tuberculosis complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification of gDNA directly from the submitted MGIT tubes, without preceding subculturing. All samples are then sequenced on an Illumina MiSeq sequencer and analyzed using an in-house developed and validated bioinformatics workflow to determine the species and antimicrobial resistance. In this study, we retrospectively compare results obtained via WGS to conventional phenotypic and genotypic testing, for all Belgian MTB strains analyzed in 2019 (n = 306). Results: In all cases, the WGS-based procedure was able to identify correctly the MTB species. Compared to MGIT drug susceptibility testing (DST), the sensitivity and specificity of genetic prediction of resistance to first-line antibiotics were respectively 100 and 99% (rifampicin, RIF), 90.5 and 100% (isoniazid, INH), 100 and 98% (ethambutol, EMB) and 61.1 and 100% (pyrazinamide, PZA). The negative predictive value was above 95% for these four first-line drugs. A positive predictive value of 100% was calculated for INH and PZA, 80% for RIF and 45% for EMB. Conclusions: Our study confirms the effectiveness of WGS for the rapid detection of M. tuberculosis complex and its drug resistance profiles for first-line drugs even when working directly on MGIT tubes, and supports the introduction of this test into the routine workflow of laboratories performing tuberculosis diagnosis.
Li+ Dynamics of Liquid Electrolytes Nanoconfined in Metal–Organic Frameworks

Metal–organic frameworks (MOFs) are excellent platforms to design hybrid electrolytes for Li batteries with liquid-like transport and stability against lithium dendrites. We report on Li+ dynamics in quasi-solid electrolytes consisting in Mg-MOF-74 soaked with LiClO4–propylene carbonate (PC) and LiClO4–ethylene carbonate (EC)/dimethyl carbonate (DMC) solutions by combining studies of ion conductivity, nuclear magnetic resonance (NMR) characterization, and spin relaxometry. We investigate nanoconfinement of liquid inside MOFs to characterize the adsorption/solvation mechanism at the basis of Li+ migration in these materials. NMR supports that the liquid is nanoconfined in framework micropores, strongly interacting with their walls and that the nature of the solvent affects Li+ migration in MOFs. Contrary to the “free’’ liquid electrolytes, faster ion dynamics and higher Li+ mobility take place in LiClO4–PC electrolytes when nanoconfined in MOFs demonstrating superionic conductor behavior (conductivity σrt > 0.1 mS cm–1, transport number tLi+ > 0.7). Such properties, including a more stable Li electrodeposition, make MOF-hybrid electrolytes promising for high-power and safer lithium-ion batteries.
SIRT1‐mediated deacetylation of FOXO3a transcription factor supports pro‐angiogenic activity of interferon‐deficient tumor‐associated neutrophils

Angiogenesis plays an important role during tumor growth and metastasis. We could previously show that Type I interferon (IFN)‐deficient tumor‐associated neutrophils (TANs) show strong pro‐angiogenic activity, and stimulate tumor angiogenesis and growth. However, the exact mechanism responsible for their pro‐angiogenic shift is not clear. Here, we set out to delineate the molecular mechanism and factors regulating pro‐angiogenic properties of neutrophils in the context of Type I IFN availability. We demonstrate that neutrophils from IFN‐deficient (Ifnar1−/−) mice efficiently release pro‐angiogenic factors, such as VEGF, MMP9 or BV8, and thus significantly support the vascular normalization of tumors by increasing the maturation of perivascular cells. Mechanistically, we could show here that the expression of pro‐angiogenic factors in neutrophils is controlled by the transcription factor forkhead box protein O3a (FOXO3a), which activity depends on its post‐translational modifications, such as deacetylation or phosphorylation. In TANs isolated from Ifnar1−/− mice, we observe significantly elevated SIRT1, resulting in SIRT1‐mediated deacetylation of FOXO3a, its nuclear retention and activation. Activated FOXO3a supports in turn the transcription of pro‐angiogenic genes in TANs. In the absence of SIRT1, or after its inhibition in neutrophils, elevated kinase MEK/ERK and PI3K/AKT activity is observed, leading to FOXO3a phosphorylation, cytoplasmic transfer and inactivation. In summary, we have found that FOXO3a is a key transcription factor controlling the angiogenic switch of neutrophils. Post‐translational FOXO3a modifications regulate its transcriptional activity and, as a result, the expression of pro‐angiogenic factors supporting development of vascular network in growing tumors. Therefore, targeting FOXO3a activity could provide a novel strategy of antiangiogenic targeted therapy for cancer.
Case Study on the Effect of 690 mpa (100 ksi) Steel Reinforcement on Concrete Productivity in Buildings

AbstractHigh-strength steel reinforcement in buildings had previously been limited to specialized applications, but recently published design guidance allows expanded use. A case study, conducted to investigate productivity benefits of using 690 mpa (100 ksi) versus 414 mpa (60 ksi) reinforcement, found that there was little to no benefit in using 690 mpa (100 ksi) steel in slabs, post-tensioned girders, and columns, but the beam reinforcement weight was reduced by 36%. The 2010 material cost ratio of 690 mpa (100 ksi) to 414 mpa (60 ksi) reinforcement was two, outstripping the weight reduction. However, labor cost is a function of weight, bringing the overall cost of 690 mpa (100 ksi) reinforcement to within 35% of 414 mpa (60 ksi) reinforcement cost. The material cost ratio will presumably decrease over time; if it drops by 30% or more, 690 mpa (100 ksi) reinforcement will be more economical. Labor costs, which vary by location, strongly influence the productivity benefits of 690 mpa (100 ksi) reinforce.
The expression of FOXP3 in lesions of several forms of leprosy in patients co-infected with HIV

Background Brazil remains endemic for infection by the human immunodeficiency virus (HIV) and leprosy, having a major impact on public health and the life quality of affected patients. Although the relevance of this co-infection is recognized, several aspects, such as the immune response, are not yet fully understood. The objective of this study was to investigate the expression of FOXP3+ Treg cells in leprosy skin lesions and to correlate their clinical forms, laboratory characteristics (CD4, CD8, and CV), and the immune reconstitution syndrome in HIV-leprosy co-infection. Methodology/Principal findings An observational, cross-sectional, and analytical study was carried out comparing four groups of patients: those with concomitant diagnosis of leprosy and HIV infection without a leprosy reaction, those with leprosy and HIV co-infection patients with a reverse reaction (RR), those with leprosy without HIV and without reaction, and those with leprosywithout HIV and with RR. The patients were diagnosed at a dermatology outpatient clinic located in Belém, Pará, Brazil, from 2003 to 2017. In the sample studied, there was a positive correlation between FOXP3+ cell density and viral load, negative correlation with blood CD4+ (not statistically significant), significant positive correlation in CD8 count in patients with leprosy reaction, and positive relationship in patients with IRIS. The density of cells expressing FOXP3 was higher in the BL/LL forms in patients without HIV, although the difference was not statistically significant. However, the cell mean was higher in the TT/BT forms in patients co-infected with leprosy and HIV, showing contradictory results. Conclusions/Significance These findings support that higher activity of the HIV may stimulate or result in a higher expression of FOXP3-Tregs and that they may be involved in active immunosuppression observed at the infection site at the tissue level. This supports the need to expand studies on FOXP3+ Treg cells in co-infected patients.
Information and communication technology to improve school participation among upper secondary school students with special educational needs

Abstract Background Evidence-based information and communication technology (ICT) interventions to enhance school participation among students with special educational needs (SEN) are required. Aim To evaluate the impact of an ICT intervention on school participation among upper secondary school students with special educational needs. Materials and methods Data on 300 students’ assessments with the School Setting Interview (SSI), grades and school attendance were used in this quasi-experimental study, with a one-group pretest-posttest design. Descriptive and inferential statistics and effect size were used, as well as Rasch analysis to generate interval data on the students’ ordinal SSI ratings. Results In the ICT intervention (median time eight hours), 54% of the students received a tablet, and software concerned with planning and structure was received by 85%. After the ICT intervention, a significant decrease in perceived need for adjustments in school activities was found on a group level and 30% of the students improved their school attendance. Students who benefitted the most from the ICT intervention had few adjustment needs in school activities and no special educational support at baseline. Conclusion An ICT intervention is promising for improving school participation among upper secondary school students with SEN.
Effects of plyometric jump training versus power training using free weights on measures of physical fitness in youth male soccer players

ABSTRACT This study aimed to contrast the effects of power training (PT) and plyometric-jump-training (PJT) programmes on measures of physical fitness in prepubertal male soccer players. Thirty-three participants were randomly allocated to PT group (n = 11), PJT group (n = 11), and an active control group (CG; n = 11). Before and after 12 weeks of training, tests were performed for the assessment of sprint-speed , change-of-direction (CoD) speed, muscular strength , and aerobic-endurance (AE). Findings indicated significant group×time interaction effects for all sprint-speed intervals, CoD speed, AE, and strength (d = 0.20–0.32). Post-hoc analyses revealed significant, moderate-to-large improvements in all sprint-speed intervals, CoD speed, AE, and muscle strength following PT (ES = 0.71 to 1.38). The PJT induced significant, moderate-to-large enhancements in 10 m, 20 m, and 30 m sprint, CoD speed, and AE (ES = 0.51 to 0.96) with no significant changes for 5 m sprint-speed and muscle strength (ES = 0.71 and 0.16, respectively). No significant pre-post changes were observed for the CG . Overall, PT and PJT are effective means to improve various measures of physical fitness in prepubertal male soccer players. Notably, to additionally improve acceleration and muscle strength, PT has an advantage over PJT.
Explaining regional differences in mortality during the first wave of Covid-19 in Italy

Italy was hit harshly by the Covid-19 pandemic, registering more than 35,000 Covid-19 deaths between February and July 2020. During this first wave of the epidemic, the virus spread unequally across the country, with northern regions witnessing more cases and deaths. We investigate demographic and socio-economic factors contributing to the diverse regional impact of the virus during the first wave. Using generalized additive mixed models, we find that Covid-19 mortality at regional level is negatively associated with the degree of intergenerational co-residence, number of intensive care unit beds per capita, and delay in the outbreak of the epidemic. Conversely, we do not find strong associations for several variables highlighted in recent literature, such as population density or the share of the population who are older or have at least one chronic disease. Our results underscore the importance of context-specific analysis for the study of a pandemic.
Epidemiological study of snakebite cases in Sikkim: Risk modeling with regard to the habitat suitability of common venomous snakes

Background Snakebite envenoming is listed as category ‘A’ Neglected Tropical Disease. To achieve the target of WHO (World Health Organization) 2019, it becomes necessary to understand various attributes associated with snakebite including community awareness, improvisation of medical facilities and to map the potential distribution of venomous snakes responsible for the bite. Hence this study is conducted in Sikkim, India to understand the epidemiology of snakebite in Sikkim. The potential distribution and risk mapping of five common venomous snakes are done for effective management of snakebite cases. Methods and findings The snakebite cases registered in six district hospitals and four PHCs (Primary Health Centers) of Sikkim were collected from the year 2011 to 2018. Community survey was also conducted to supplement the data. Ecological Niche Modeling (ENM) was performed to predict the potential habitat of five common venomous snakes of Sikkim. The risk modeling of snakebite cases was done at the level of Gram Panchayat Unit (GPU) using Geographically Weighted Regression (GWR) and Ordinary Linear Square (OLS) model. We found higher number of male victims inflicted with snakebite envenomation. The potential distribution of the five venomous snakes showed satisfactory mean AUC (Area under Curve) value. Both the models showed significant positive association of snakebite cases with habitat suitability of the venomous snakes. Hospital data revealed no death cases whereas community data reported 24 deaths. Conclusions Death from snakebite reflected in community data but not in hospital data strongly indicates the people’s belief in traditional medicine. Though people of Sikkim have rich traditional knowledge, in case of snakebite traditional practices may be ineffective leading to loss of life. Sensitizing people and improving medical facilities along with proper transport facilities in rural areas might significantly reduce the snakebite casualties in the state.
The Effects of Time Delay and Physical Motion on Manual Flight Control: An In-Flight and Ground-Based Simulation Experiment

An experiment addressed the effects of time delay and physical motion on manual flight control and flying qualities ratings. Flight tasks were presented on a head-up display in a variable-stability NT-33A aircraft. Specified maneuvers were performed under simulated instrument meteorological conditions with variable signal delays added to the flight control system. To assess the effects of physical motion, the same experiment was replicated with the airplane parked on the ground using a digital aerodynamic simulation. Increasing time delay degraded tracking performance more in the no-motion ground-based simulation than in the full-motion in-flight simulation. Similar results occurred for flying qualities ratings.
Detection of melanogenesis- and anti-apoptosis-associated melanoma factors: Array CGH and PPI mapping integrating study.

BACKGROUND We investigated melanogenesis- and anti-apoptosis-related melanoma factors in melanoma cells (TXM1, TXM18, A375P, and A375SM). OBJECTIVE To find melanoma associated hub factor, high-throughput screening-based techniques integrating with bioinformatics were investigated. METHODS Array CGH analysis was conducted with a commercial system. Total genomic DNAs prepared individually from each cell line with control DNA were properly labeled with Cy3-dCTP and Cy5-dCTP and hybridizations and subsequently performed data treatment by the log2 green (G; test) to red (R; reference) fluorescence ratios (G/R). Gain or loss of copy number was judged by spots with log2-transformed ratios. PPI mapping analysis of detected candidate genes based on the array CGH results was conducted using the human interactome in the STRING database. Energy minimization and a short molecular dynamics (MD) simulation using the implicit solvation model in CHARMM were performed to analyze the interacting residues between YWHAZ and YWHAB. RESULTS Three genes (BMP-4, BFGF, LEF-1) known to be involved in melanogenesis were found to lose chromosomal copy numbers, and Chr. 6q23.3 was lost in all tested cell lines. Ten hub genes (CTNNB1, PEX13, PEX14, PEX5, IFNG, EXOSC3, EXOSC1, EXOSC8, UBC, and PEX10) were predicted to be functional interaction factors in the network of the 6q23.3 locus. The apoptosis-associated genes E2F1, p50, BCL2L1, and BIRC7 gained, and FGF2 lost chromosomal copy numbers in the tested melanoma cell lines. YWHAB, which gained chromosomal copy numbers, was predicted to be the most important hub protein in melanoma cells. Molecular dynamics simulations for binding YWHAB and YWHAZ were conducted, and the complex was predicted to be energetically and structurally stable through its 3 hydrogen-bond patterns. The number of interacting residues is 27. CONCLUSION Our study compares genome-wide screening interactomics predictions for melanoma factors and offers new information for understanding melanogenesis- and anti-apoptosis-associated mechanisms in melanoma. Especially, YWHAB was newly detected as a core factor in melanoma cells.
NeuroGrasp: Real-Time EEG Classification of High-Level Motor Imagery Tasks Using a Dual-Stage Deep Learning Framework

Brain–computer interfaces (BCIs) have been widely employed to identify and estimate a user’s intention to trigger a robotic device by decoding motor imagery (MI) from an electroencephalogram (EEG). However, developing a BCI system driven by MI related to natural hand-grasp tasks is challenging due to its high complexity. Although numerous BCI studies have successfully decoded large body parts, such as the movement intention of both hands, arms, or legs, research on MI decoding of high-level behaviors such as hand grasping is essential to further expand the versatility of MI-based BCIs. In this study, we propose NeuroGrasp, a dual-stage deep learning framework that decodes multiple hand grasping from EEG signals under the MI paradigm. The proposed method effectively uses an EEG and electromyography (EMG)-based learning, such that EEG-based inference at test phase becomes possible. The EMG guidance during model training allows BCIs to predict hand grasp types from EEG signals accurately. Consequently, NeuroGrasp improved classification performance offline, and demonstrated a stable classification performance online. Across 12 subjects, we obtained an average offline classification accuracy of 0.68 (±0.09) in four-grasp-type classifications and 0.86 (±0.04) in two-grasp category classifications. In addition, we obtained an average online classification accuracy of 0.65 (±0.09) and 0.79 (±0.09) across six high-performance subjects. Because the proposed method has demonstrated a stable classification performance when evaluated either online or offline, in the future, we expect that the proposed method could contribute to different BCI applications, including robotic hands or neuroprosthetics for handling everyday objects.