Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecules in Focus Eukaryotic initiation factor 4E

Eukaryotic translation initiation factor 4E (eIF4E) is perhaps best known for its function in the initiation of protein synthesis on capped mRNAs in the cytoplasm. However, recent studies have highlighted that eIF4E has many additional functions, which include the nuclear export of specific mRNAs as well as roles in ageing and the translation of some uncapped viral RNAs. This review aims to update the reader on recent developments, including the potential of eIF4E as a therapeutic target.
HIV SEXUAL RISK IN OLDER BLACK WOMEN: A SYSTEMATIC REVIEW

Dr. Tanyka K. Smith, PhD, FNP-BC and Postdoctoral Research Fellow at Columbia University School of Nursing, 617 West 168th Street, New York, NY 10032, (Cell) 347-224-5351, (Work) 212-342-0851, (Fax) 212-305-0723. Dr. Smith was supported as a postdoctoral trainee by the National Institute of Nursing Research, National Institutes of Health (Training in Interdisciplinary Research to Prevent Infections, T32 NR013454)
Physician Workforce and Healthcare Markets Employed Family Physician Satisfaction and Commitment to Their Practice, Work Group, and Health Care Organization

Objective. Test a model of family physician job satisfaction and commitment. Data Sources/Study Setting. Data were collected from 1,482 family physicians in a Midwest state during 2000–2001. The sampling frame came from the membership listing of the state’s family physician association, and the analyzed dataset included family physicians employed by large multispecialty group practices. Study Design and Data Collection. A cross-sectional survey was used to collect data about physician working conditions, job satisfaction, commitment, and demographic variables. Principal Findings. The response rate was 47 percent. Different variables predicted the different measures of satisfaction and commitment. Satisfaction with one’s health care organization (HCO) was most strongly predicted by the degree to which physicians perceived that management valued and recognized them and by the extent to which physicians perceived the organization’s goals to be compatible with their own. Satisfaction with one’s workgroup was most strongly predicted by the social relationship with members of the workgroup; satisfaction with one’s practice was most strongly predicted by relationships with patients. Commitment to one’s workgroup was predicted by relationships with one’s workgroup. Commitment to one’s HCO was predicted by relationships with management of the HCO. Conclusions. Social relationships are stronger predictors of employed family physician satisfaction and commitment than staff support, job control, income, or time pressure.
Medical Records Flag for Suicide Risk: Predictors and Subsequent Care Utilization among Veterans with Substance Use Disorders

Objective: The Veterans Affairs (VA) healthcare system identified suicide prevention as a top priority and established policies to include high-risk suicide patient record flags (PRFs) in the electronic medical record to alert providers of patient risk and increase healthcare contacts. This study identified predictors of new PRFs and described healthcare utilization before and after PRF initiation among VA patients with substance use disorders. Methods: The sample included patients ages 18 and older who received a substance use disorder diagnosis in 2012 (N=474,946). Demographic, clinical and utilization predictors of PRFs were identified using multivariable logistic regression. Changes in short-term (3-months) and longer-term (12 months) healthcare utilization before and after PRF activation were compared using negative binomial regression. Results: A total of 8,913 patients received PRFs. Demographic predictors of PRF initiation included being younger than 35, White, and homeless. Clinical predictors were cocaine, opioid and sedative use disorders, posttraumatic stress, psychotic, bipolar, and depressive disorders, and suicide-attempt diagnoses. Patients with PRFs averaged 1.33 (95%Confidence Interval (CI): 3.80– 4.42) times more primary care, 2.29 (95%CI: 2.242.34) times more mental health, 4.10 (95%CI: 3.80–4.42) times more substance use clinic visit days, and fewer (.55, 95%CI: .53–.58) emergency department visit days in the 3 months following compared to 3 months before PRF initiation. Modest increases in mental health and substance use-related days hospitalized were observed. Conclusion: Veterans identified with PRFs received significantly more healthcare services after PRF initiation. Further research is warranted on the effects of PRFs on clinical outcomes, suicide behaviors.
A Novel Approach using Actigraphy to Quantify the Level of Disruption of Sleep from In-Home Polysomnography: The MrOS Sleep Study

Background— The “first night effect” of polysomnography (PSG) has been studied, however the ability to quantify the level of sleep disruption has been confounded by using PSG on all nights. We used actigraphy to quantify disruption level, and examined characteristics associated with disruption. Methods— 778 older men (76.2 ± 5.4 years) from a population-based study at six US centers underwent one night of in-home PSG. Actigraphy was gathered on the PSG night and three subsequent nights. Actigraphically measured total sleep time (TST), sleep efficiency (SE), wake after sleep onset (WASO), and sleep onset latency (SOL) were compared from the PSG night and subsequent nights. Linear regression models were used to examine the association of characteristics and sleep disruption. Results— On average, sleep on the PSG night was worse than the following night (p<0.05, TST 21 ± 85 min less, SE 2.3 ± 11.3% less, WASO 4.9 ± 51.8 min more, SOL 6.6 ± 56.2 min more). Compared to sleep two and three nights later, sleep on the PSG night was significantly worse. Characteristics associated with greater sleep disruption on the PSG night, included older age, higher apnea-hypopnea index, worse neuromuscular function, and more depressive symptoms. Minorities and men with excessive daytime sleepiness slept somewhat better on the PSG night. Conclusions— Among older men, there was sleep disruption on the PSG night which may lead to an underestimation of sleep time. The increase of sleep on the night after the PSG suggests data from the second monitoring may overestimate sleep.
Nucleic Acids Research

The human immunoglobulin pseudo-gamma IGHGP gene shows no major structural defect The human immunoglobulin heavy chain constant region genes have been sequenced (1), except the pseudogene IGHGP for which only the hinge is known (2, 3). The GP gene has previously been localized between the Al and G2 genes by gene deletion mapping (4, 5) and shown to lack the switch region (3). In this paper, we present the sequence of the GP gene isolated from an individual homozygous for the GP*1 allele defined by RFLP (N. Ghanem, M.-P.L and G.L. in preparation). Our data show that there is no major structural defect in the GP gene itself. Only noticeable is a deletion of three nucleotides which overlaps two codons and results in the loss of the aminoacid at position 15 in the CH3 exon and in the change of the following aminoacid, but which does not alter the open reading frame. The splicing sites (arrows) and the polyadenylation site (underlined) are conserved as well as the cysteins involved in the intradisulfide bonds. Therefore the lack of switch region seems to be the only defect responsible for the absence of protein encoded by the IGHGP gene.
Plasmodium falciparum GPI mannosyltransferase-III is functional with novel signature sequence

The glycosylphosphatidylinositol (GPI) anchors of Plasmodium falciparum are indispensable for parasite survival since merozoite surface proteins-1, -2, -4, -5 and -10 crucial for erythrocyte invasion are GPI-anchored. Therefore, the GPI biosynthetic pathway can offer potential targets for novel anti-malarial drugs. Here, we characterized the putative P. falciparum PIG-B gene ( PfPIGB ) that encodes mannosyltransferase-III of GPI biosynthesis. PfPIGB mRNA is transcribed in a developmental stage specific manner. A protein corresponding to the expected size of PfPIG-B is expressed by the parasite and is localized in the endoplasmic reticulum. Treatment of parasites with PfPIG-B specific siRNA caused reduced GPI synthesis, affecting the PIG-B specific GPI intermediate. These data demonstrate that PfPIG-B is functional and encodes for mannosyltransferase-III of the parasite GPI biosynthesis. The parasite PfPIG-B is novel in that its signature sequence HKEHKI is unique and is only partially conserved compared to HKEXRF signature motif of mammalian PIG-B enzymes.
Growth Factors, Cytokines, Cell Cycle Molecules p21 Is Required for the Development of Monocytes and Their Response to Serum Transfer-induced Arthritis

From the Department of Molecular Microbiology and Immunology * and the Department of Medicine, Division of Rheumatology, School of Medicine, St. Louis University, St. Louis, Missouri; the Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine, Midwestern University, Downers Grove, Illinois; and the Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois
The Effect of Burn Injury on Serum Concentrations of Sclerostin and FGF23

Severe burn injury results in acute bone resorption followed by an adynamic state, most likely due to changes brought about by the inflammatory and glucocorticoid responses to the injury. There is a consequent increase in annual extrapolated fracture incidence in children. While osteoblasts have been reported to disappear from the bone surface and stem cell differentiation into osteoblasts is impaired, the effect of burns on osteocyte function is unknown. We measured serum concentrations of two osteocyte proteins, sclerostin and fibroblast growth factor (FGF)-23 between 6–60 days post-burn in pediatric patients, ages 5–18 years who had participated in a randomized controlled double-blind study of acute administration of pamidronate to prevent the resorptive bone loss. While FGF-23 was undetectable in all samples, the plot of sclerostin concentration versus time post-burn yielded a statistically significant difference between slopes, −2.5 in the placebo control group and +3.5 in the group receiving pamidronate, p=0.016 by ANCOVA. The FGF23 data suggest that osteocytes may be apoptotic, although the sclerostin data may indicate partial preservation of osteocyte function in subjects receiving pamidronate or an ectopic source of sclerostin.
MR arthrography evaluation in [17_TD$DIFF] patients with traumatic anterior shoulder instability

Introduction: To evaluate retrospectively the diagnostic accuracy of MR [2_TD$DIFF] arthrography, compared with arthroscopy, in research of [24_TD$DIFF] the glenoid labrum tear in patients with a ﬁrst episode of traumatic anterior shoulder instability (TUBS). Material and [25_TD$DIFF] methods: We retrospectively reviewed the MR [2_TD$DIFF] arthrography shoulder images of 118 patients with a ﬁrst episode of TUBS, between June 2014 and May 2016. Results: The overall accuracy of MR [2_TD$DIFF] arthrography compared with arthroscopy of the glenoid labrum lesions was 94%, sensitivity 93%, [26_TD$DIFF] and speciﬁcity 96%. The sensitivity of MR [2_TD$DIFF] arthrography for Perthes lesion was 71.4%, [27_TD$DIFF] and for ALPSA lesion, it was 91%. Slap lesion occurred in 11 out of 77 cases (9.3% of 118 cases). The Hill-Sachs lesion occurred in 48 [28_TD$DIFF] out of 118 cases (40.7%), while the Hill-Sachs reverse lesion in 4 [29_TD$DIFF] cases (3.4%). Conclusions: The MR [30_TD$DIFF] arthrography is accurate in detecting labral injuries. However, other [31_TD$DIFF] studies are needed to assess the less frequent tear, as Perthes lesion. The presence of the Hill [32_TD$DIFF] -Sachs lesion could provide useful information about the level of the shoulder instability.
Platelet Recruitment to the Inflamed Glomerulus Occurs via an (cid:1) IIb (cid:2) 3 /GPVI-Dependent Pathway

Recruitment of leukocytes to glomeruli is fundamen-tal to the pathogenesis of many forms of glomerulonephritis. In a model of glomerulonephritis induced by in situ immune complex deposition, we previously observed that, in addition to leukocytes, platelets accumulate in glomerular capillaries, where they contribute to leukocyte recruitment. However, the mechanisms of platelet recruitment and the role of platelet-expressed P-selectin in leukocyte recruitment require further investigation. We used intravital microscopy to examine the mechanisms of platelet and leukocyte recruitment to glomeruli of mice following administration of an antibody against the glomerular basement membrane (anti-GBM antibody). Platelet recruitment was initiated within five minutes of administration of anti-GBM antibody. This was unaltered by inhibition of platelet GPIb (cid:1) but was prevented by the absence of platelet GPVI. Fibrinogen was deposited in glomerular capillaries via a partially intercellular adhesion molecule 1 (ICAM-1)–dependent mechanism, and inhibition of (cid:1) IIb (cid:2) 3 , fibrinogen and ICAM-1 inhibited platelet recruitment. Notably, neutrophil depletion also reduced platelet accumulation, indicating a cooperative interaction underlying recruitment of platelets and neutrophils. roles in leukocyte recruitment and resultant glomerular injury. Together these findings suggested the existence of a unique mechanism for leukocyte recruitment in this vascular bed.
Nucleic Acids Research Nucleotide sequence of Candida pelliculosa (3-glucosidase gene

The nucleotide sequence of the DNA fragment containing the a-glucosidase gene of Candida pelliculosa was determined.Analysis of the sequence revealed three open reading frames which could encode 65,825,and 412 amino acid residues. The presence of the second frame was found to be sufficient for the expression of the a-glucosidase gene in a heterologous host Saccharomyces cerevisiae. Putative protein encoded by this gene had hydrophobic amino acids, resembling a signal peptide, at its N-terminal region and 19 potential glycosylation sites. Codon usage of Candida genes had the similar pattern shown in S.cerevisiae. Codon bias of the a-glucosidase gene of Candida was relatively low,compared with that of the highly expressed genes of S.cerevisiae. INTRODUCTION a-Glucosidase is distributed widely throughout prokaryotes and eukaryotes. The role of this enzyme in the cell is not known but it is one of the enzymes responsible for the degradation of cellulosic materials. From the point of view of the application of this enzyme,O-glucosidase have been isolated from various organisms and their characteristics were studied (1-3). Recently, recombinant DNA technique was also introduced into this field. 6Glucosidase of Escherichia adecapsulata was cloned and expressed in E.coli (4). Penttila et al. (5) reported that a-glucosidase of Aspergillus niger was cloned into the host S.cerevisiae by using chromogenic sbstrate, 5-bromo4-chloro-3-indolyl-6-D-glucopyranoside (X-glu),however, its production was not strong enough to be detected in vitro. Candida pelliculosa can assimilate cellobiose aerobically and a-glucosidase produced by this yeast may participate in this metabolic reaction. Properties of the purified enzyme from C.pelliculosa were studied in our laboratory (6). The enzyme is a glycoprotein and is composed of four subunits of molecular weight 90,000. Its isoelectric point was estimated to be 4.9. It could act on p-nitrophenyl-a-D-glucoside (pNPG) and cellobiose. No other a) I R L Press Limited, Oxford, England. 6273 Nucleic Acids Research glucosidase was detected in C.pelliculosa. Using C.pelliculosa as the gene donor, we have cloned the 6-glucosidase gene of Candida in a S.cerevisiae host (to be published elsewhere). In this paper, we present the nucleotide sequence of the cloned gene, including the entire coding region of the 6-glucosidase gene and its 5' and 3' flanking
The effects of age and LPS-mediated peripheral inflammation on numbers of central catecholaminergic neurons

Parkinson’s disease (PD), an age-related movement disorder, is characterized by severe catecholaminergic neuron loss in the substantia nigra pars compacta-ventral tegmental area (SNPC-VTA) and locus coeruleus (LC). To assess the stability of these central catecholaminergic neurons following an acute episode of severe inflammation, 6 to 22 month-old C57/Bl6 mice received a maximally tolerated dose of LPS followed by euthanasia two hours later to assay peak levels of peripheral and central cytokines; and, 14 weeks later for computerized stereology of tyrosine hydroxylase-immunopositive (TH+) neurons in the SNPC-VTA and LC. Two hours after LPS, cytokine levels varied in an age-related manner, with the greatest peripheral and central elevations in old and young mice, respectively. Severe inflammation failed to cause loss of TH+ neurons in SNPC-VTA or LC; however, there was an age-related decline in these TH+ neurons in LPS-treated and control groups. Thus, unknown mechanisms in the B6 mouse brain appear to protect against catecholaminergic neuron loss during an acute episode of severe inflammation, while catecholaminergic neuron loss occurs during normal aging. Send correspondence to: Peter R. Mouton, Ph.D., Department of Pathology & Cell Biology, Byrd Alzheimer’s Center and Research Institute at the University of South Florida College of Medicine, Room 311, 4001 E Fletcher Ave, Tampa, Florida 33613-4808; 813-396-0662 (office); 410-490-2036 (cell); 866-262-4901 (fax); pmouton@health.usf. edu. Disclosure Statement No actual or potential conflicts of interest are present for any of the authors and the work presented in this report. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author Manuscript Neurobiol Aging. Author manuscript; available in PMC 2013 February 1. Published in final edited form as: Neurobiol Aging. 2012 February ; 33(2): 423.e27–423.e36. doi:10.1016/j.neurobiolaging. 2010.09. 025. N IH PA Athor M anscript N IH PA Athor M anscript N IH PA Athor M anscript
Estimation of nitric oxide synthase activity via LC-MS/MS determination of 15 N 3 -citrulline in biological samples

Rationale— We showed that the metabolite peaks of 15 N 3 -citrulline ( 15 N 3 -CIT) and 15 N 3 arginine ( 15 N 3 -ARG) could be detected when 15 N 4 -ARG was metabolized by nitric oxide synthase (NOS) in endothelial cells. The usefulness of these metabolites as potential surrogate indices of nitric oxide (NO) generation is evaluated. Methods— A hydrophilic-interaction liquid chromatography electrospray tandem mass spectrometric assay (LC-MS/MS) was utilized for the simultaneous analysis of 15 N 4 -ARG, ARG, CIT, 15 N 3 -CIT and 15 N 3 -ARG. 15 N 3 -CIT and 15 N 3 -ARG from impurities of 15 N 4 -ARG were determined and corrected for the calculation of their concentration. 15 N 4 -ARG-derived NO, i.e., 15 NO formation was determined by analyzing 15 N-nitrite accumulation by another LC-MS/MS assay. Results— After EA.hy926 human endothelial cells were challenged with 15 N 4 -ARG for 2 hours, the peak intensities of 15 N 3 -CIT and 15 N 3 -ARG significantly increased with 15 N 4 -ARG concentration and positively correlated with 15 N-nitrite production. The estimated Km values were independent of the metabolite (i.e., 15 N 3 -CIT, 15 N 3 -CIT+ 15 N 3 -ARG or 15 N-nitrite) used for calculation. However, after correction for its presence as a chemical contaminant of 15 N 4 ARG, 15 N 3 -ARG was only a marginal contributor for the estimation of NOS activity. Conclusions— These data suggest that the formation of 15 N 3 -CIT can be used as an indicator of NOS activity when 15 N 4 -ARG is used as a substrate. This approach may be superior to the radioactive 14 C-CIT method which can be contaminated by 14 C-urea, and to the 14 N-nitrite method which lacks sensitivity.
Expression and Role of Adhesion Molecule CD1 8 on Bovine Neutrophils

RESUME INTRODUCTION Expression of CD18 on bovine neutrophils in response to stimulation by zymosan activated serum (ZAS) and phorbol myristate acetate (PMA) and the effects of monoclonal antibodies (MAB) recognizing CD18 or bovine neutrophil surface antigens (S2G8 and S5F8G10) on adherence, chemotactic responses and phagocytosis of bovine neutrophils were evaluated. CD18 expression of neutrophils was increased after ZAS and PMA treatment by 12.2 and 54.2% respectively, and were significantly (p < 0.05, p < 0.01) different from those of untreated neutrophils. CD18 expression by neutrophils from a Holstein-Friesian heifer affected with leukocyte adhesion deficiency was within negative controls when stimulated by ZAS and PMA.
The effects of patient-centered depression care on patient satisfaction and depression remission

Background. While health systems are striving for patient-centered care, they have little evidence to guide them on how to engage patients in their care, or how this may affect patient experiences and outcomes. Objective. To explore which specific patient-centered aspects of care were best associated with depression improvement and care satisfaction. Methods. Design: observational. Setting: 83 primary care clinics across Minnesota. Subjects: Primary care patients with new prescriptions for antidepressants for depression were recruited from 2007 to 2009. Outcome measures: Patients completed phone surveys regarding demographics and self-rated health status and depression severity at baseline and 6 months. Patient centeredness was assessed via a modified version of the Patient Assessment of Chronic Illness Care. Differences in rates of remission and satisfaction between positive and negative responses for each care process were evaluated using chi-square tests. Results. At 6 months, 37% of 792 patients ages 18–88 achieved depression remission, and 79% rated their care as good-to-excellent. Soliciting patient preferences for care and questions or concerns, providing treatment plans, utilizing depression scales and asking about suicide risk were patient-centered measures that were positively associated with depression remission in the unadjusted model; these associations were mildly weakened after adjustment for depression severity and health status. Nearly all measures of patient centeredness were positively associated with care ratings. Conclusion. The patient centeredness of care influences how patients experience and rate their care. This study identified specific actions providers can take to improve patient satisfaction and depression outcomes.