Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of iron and nitrogen on the development of Helminthosporium solani and potato silver scurf

Abstract Silver scurf is a surface blemish disease of potato (Solanum tuberosum L.) tubers caused by Helminthosporium solani Durieu & Mont. Silver scurf is becoming a disease of high economic impact. In this study, the effect of different iron (FeSO4, FeCl2) and nitrogen (NaNO2, NaNO3, NH4Cl, NH4NO3) salts on H. solani conidial germination and on potato silver scurf development was evaluated. The results show that iron and nitrogen salts affect in vitro germination of H. solani conidia. Conidia were particularly sensitive to FeSO4 and FeCl2. These salts completely inhibited conidial germination and were shown to be toxic at a concentration of 0.9 mM. Among the nitrogen salts tested, conidia were most affected by NaNO2 and NH4Cl, which almost completely inhibited their germination at a concentration of 169.7 mM. NaNO2 was also toxic to conidia. Among the salts tested, only FeSO4, FeCl2 and NaNO2 reduced the development of silver scurf. Comparison of the effect of the different tested salts leads to the conclusion that ions Fe++ and NO2 − have toxic effects on the conidia and repressive effects on silver scurf development while NO3 − and NH4 + have no toxic effect on conidia and no repressive effect on silver scurf development.
The prevalence of noma in northwest Nigeria

Background Noma, a rapidly progressing infection of the oral cavity, mainly affects children. The true burden is unknown. This study reports estimated noma prevalence in children in northwest Nigeria. Methods Oral screening was performed on all ≤15 year olds, with caretaker consent, in selected households during this cross-sectional survey. Noma stages were classified using WHO criteria and caretakers answered survey questions. The prevalence of noma was estimated stratified by age group (0–5 and 6–15 years). Factors associated with noma were estimated using logistic regression. Results A total of 177 clusters, 3499 households and 7122 children were included. In this sample, 4239 (59.8%) were 0–5 years and 3692 (52.1%) were female. Simple gingivitis was identified in 3.1% (n=181; 95% CI 2.6 to 3.8), acute necrotising gingivitis in 0.1% (n=10; CI 0.1 to 0.3) and oedema in 0.05% (n=3; CI 0.02 to 0.2). No cases of late-stage noma were detected. Multivariable analysis in the group aged 0–5 years showed having a well as the drinking water source (adjusted odds ratio (aOR) 2.1; CI 1.2 to 3.6) and being aged 3–5 years (aOR 3.9; CI 2.1 to 7.8) was associated with being a noma case. In 6–15 year olds, being male (aOR 1.5; CI 1.0 to 2.2) was associated with being a noma case and preparing pap once or more per week (aOR 0.4; CI 0.2 to 0.8) was associated with not having noma. We estimated that 129120 (CI 105294 to 1 52 947) individuals <15 years of age would have any stage of noma at the time of the survey within the two states. Most of these cases (93%; n=120 082) would be children with simple gingivitis. Conclusions Our study identified a high prevalence of children at risk of developing advanced noma. This disease is important but neglected and therefore merits inclusion in the WHO neglected tropical diseases list.
Trinexapac-ethyl, Propiconazole, Iron, and Biostimulant Effects on Shaded Creeping Bentgrass

Creeping bentgrass (Agrostis stolonifera) is used extensively on temperate zone golf course greens, tees, and fairways, but often performs poorly in shade. Previous research has indicated that sequential applications of gibberellic acid (GA) inhibiting plant growth regulators (PGRs) such as trinexapac-ethyl (TE) increase cool-season turfgrass performance in 70-90% shade. This research was conducted to: 1) confi rm appropriate TE application rates and frequencies for maintaining ‘Penncross’ creeping bentgrass in dense shade in the mid-Atlantic region of the U.S.; 2) determine the effi cacy of other PGRs, biostimulants, and iron (Fe); and 3) assess whether the addition of a biostimulant with TE would have additive, synergistic, or negative effects. The other compounds tested against TE and the control were: propiconazole (PPC), iron sulfate, CPR (a seaweed and iron containing biostimulant), and a generic seaweed extract (SWE) (Ascophyllum nodosum) plus humic acid (HA) combination. These treatments were applied to 88% shaded bentgrass every 14 days from May or June through October in 2001 and 2002, with turf quality, leaf color, root strength, photochemical effi ciency, and antioxidant enzyme superoxide dismutase (SOD) activity being determined. While the quality of control plots fell below a commercially acceptable level by the second month of the trial, repeated foliar TE application provided 33% to 44% better quality throughout the experiment. Propiconazole resulted in 13% to 17% better quality through September of each year. Trinexapac-ethyl and PPC resulted in darker leaf color and increased mid-trial root strength by 27% and 29%, respectively. Canopy photochemical effi ciency and leaf SOD activity were also increased due to TE in August of both years. Treatment with Fe, CPR, or SWE+HA did not have an effect on quality, root strength, SOD, or photochemical effi ciency, but periodic increases in color were observed. The addition of CPR to TE in 2002 provided results that were not different from those of TE-alone. This and previous studies indicate that restricting leaf elongation with antiGA PGRs is of primary importance for improving shade tolerance, while treatments that increase leaf color or chlorophyll levels without restricting leaf elongation are relatively ineffective.
Efficacy of 0.5% Levofloxacin and 5.0% Povidone-Iodine Eyedrops in Reducing Conjunctival Bacterial Flora: Metagenomic Analysis

Bacteria associated with postoperative endophthalmitis mostly originate from the normal bacterial flora of the patient's conjunctiva and eyelids, so the incidence of endophthalmitis may be reduced by eliminating the ocular and adnexal flora before surgery. We assessed the effectiveness of eyedrops of 0.5% levofloxacin and 5.0% povidone-iodine (PVI) in reducing conjunctival bacterial flora by metagenomic analysis. A total of 2.4 × 106 high-quality sequencing reads were generated from 93 conjunctival samples obtained from 31 eyes scheduled for cataract surgery before prophylactic therapy (group 1), after administration of 0.5% levofloxacin eyedrops into the conjunctival sac 8 times before surgery (group 2), and at 3 minutes after instillation of 5.0% PVI solution in the conjunctival sac (group 3) followed by surgery irrigation. The alpha diversity and beta diversity results demonstrated that group 3 had the least richness and biodiversity. Corynebacterium, Pseudomonas, Staphylococcus, Acinetobacter, and Streptococcus were predominant in all samples. The relative abundance of these bacterial species was 30.94%, 27.48%, 5.26%, 4.55%, and 2.61% in group 1, 16.32%, 44.10%, 2.19%, 5.39%, and 0.97% in group 2, and 5.90%, 65.55%, 0.39%, 5.36%, and 0.10% in group 3, respectively. The most easily and difficultly eliminated were Corynebacterium and Pseudomonas, respectively. In conclusion, the metagenomic analysis using high-throughput sequencing provides a scientific way for evaluating the effectiveness of a disinfection method from the perspective of analyzing the composition and diversity of the conjunctival microbiome. Despite the use of preoperative antisepsis regimens, the ocular surface of patients receiving cataract surgery could not be rendered completely aseptic, indicating that more strict disinfection methods need to be adopted to reduce the risk for anterior chamber contamination and endophthalmitis after cataract surgery.
Abdominal volume index: a predictive measure in relationship between depression/anxiety and obesity

Background Obesity is a risk factor for mood disorder (such as depression and anxiety). We aimed to assess application of A Body Shape Index (ABSI) and abdominal volume index (AVI), as new indices of obesity to evaluate the relationship between obesity and depression/anxiety. Methods This cross sectional study was conducted on 307 overweight and obese women (249 females, 58 males) 20–60 years in Iran in 2017–2018. The anthropometric measures including weight, waist circumference, hip circumference, body fat and derived values of body mass index, waist-hip ratio, AVI and ABSI were evaluated. HADS questionnaire for depression and anxiety completed. Results Prevalence of depression was 36.1% in women; men 24.1%; overweight 28.1%; obese 36.5 % and central obesity 33.7%. Anxiety was apparent in 27.1% of overweight 30.3 % obese and 29.6% central obesity. People with depression and anxiety had higher WC, BF and AVI. ABSI had no significant correlation with depression/anxiety. The odds of depression (1.06; 95% CI, 1.02–1.12) and anxiety (1.06; 95% CI, 1.01–1.11) were elevated with increase AVI. Conclusion Our results suggested that AVI as an indirect measure of abdominal obesity along with WC and BF could be useful in predicting the relationship between obesity and depression/anxiety.
Physical activity of Korean cancer survivors is associated with age and sex

Background/Aims This study aimed to assess adherence to physical activity guidelines in cancer survivors and to investigate related factors. Methods The cross-sectional data of 39,845 community-dwelling participants aged ≥ 20 years (1,254 cancer survivors) in the Korea National Health and Nutrition Examination Survey were analyzed. The age- and sex-stratified prevalence for meeting exercise guidelines was obtained. The correlates of meeting exercise recommendations were evaluated by using complex-sample logistic regression models. Results Overall, 36.2% and 20.5% of cancer survivors met the recommendations for aerobic and resistance exercises, respectively. Cancer survivors and noncancer controls had similar low adherence to exercise recommendations in most age and sex groups, except young female cancer survivors (20 to 49 years old) who showed higher engagement in aerobic exercise than female noncancer controls in the same age group. Old cancer survivors (≥ 65 years) were less likely to engage in recommended levels of aerobic exercise than young cancer survivors (odds ratio [OR], 0.44; 95% confidence interval [CI], 0.24 to 0.79). Female cancer survivors were less likely to meet resistance exercise guidelines than male cancer survivors (OR, 0.32; 95% CI, 0.21 to 0.50). In addition, cancer survivors with current smoking status and cardiovascular disease were less likely to meet aerobic exercise recommendations. Conclusions The prevalence of meeting exercise guidelines are low in Korean cancer survivors and varied by age and sex. Old age is associated with an insufficient level of aerobic exercise, and female sex is associated with low participation in resistance exercise.
2.7 Å cryo-EM structure of vitrified M. musculus H-chain apoferritin from a compact 200 keV cryo-microscope

Here we present the structure of mouse H-chain apoferritin at 2.7 Å (FSC = 0.143) solved by single particle cryogenic electron microscopy (cryo-EM) using a 200 kV device, the Thermo Fisher Glacios®. This is a compact, two-lens illumination system with a constant power objective lens, without any energy filters or aberration correctors, often thought of as a “screening cryo-microscope”. Coulomb potential maps reveal clear densities for main chain carbonyl oxygens, residue side chains (including alternative conformations) and bound solvent molecules. We used a quasi-crystallographic reciprocal space approach to fit model coordinates to the experimental cryo-EM map. We argue that the advantages offered by (a) the high electronic and mechanical stability of the microscope, (b) the high emission stability and low beam energy spread of the high brightness Field Emission Gun (X-FEG), (c) direct electron detection technology and (d) particle-based Contrast Transfer Function (CTF) refinement have contributed to achieving high resolution. Overall, we show that basic electron optical settings for automated cryo-electron microscopy imaging can be used to determine structures approaching atomic resolution.
The width of keratinized mucosa around dental implants and its influencing factors.

BACKGROUND A few evidence is available in the literature concerning the pattern of variation in the width of keratinized mucosa (KMW) around dental implants and factors that may affect the KMW. PURPOSE The purpose of this study is to investigate the KMW at the buccal aspect of dental implants and to analyze its influencing factors. MATERIALS AND METHODS The current study was a retrospective study conducted on 726 patients with 1252 dental implants. The following parameters were evaluated by reviewing the medical records of each patient, including the age, gender and smoking status of each patient, the reasons of teeth loss, the position of implants, the bone augmentation procedures, and the KMW. Binary logistic regression analysis with the generalized estimating equations was utilized to analyze the factors that may affect the KMW of dental implants. RESULTS The KMW of implants located in the maxilla was significantly higher than that of implants located in the mandible (P < .01), except for the upper and lower canines. The logistic regression analysis indicated that the risk of the implants presenting inadequate KMW (<2 mm) in the periodontitis-caused tooth loss group was 1.91 times of the non-periodontitis-caused tooth loss group. The risk of implants presenting inadequate KMW after receiving simple and complex bone augmentation procedures was 1.65 and 2.62 times of the risk of implants without bone augmentation, respectively. The longer the follow-up period, the higher the risk of implants presenting inadequate KMW will be. CONCLUSIONS The KMW at the buccal aspect of implants is related to the position of implants. Tooth loss due to periodontitis, the bone augmentation procedures, and the process of functional period would increase the risk of implants presenting an inadequate amount of keratinized mucosa.
A Commercial Scale Study of the Efficiency of Propionic Acid and Calcium Propionate as Fungistats in Poultry Feed

Abstract The effect of propionic acid and calcium propionate as mold inhibitors and heating preventors in poultry feed stored under Israeli summer conditions was studied in a commercial-scale study. Heating processes in untreated feed and in feed treated with calcium propionate . 5% (w/v) started after 19 and 26 days of storage, respectively, while in feed treated with propionic acid . 3% (w/v) heating started after 53 days. Maximum CO2 levels in untreated feed and in feed treated with propionic acid or calcium propionate were 18%, 12.2%, and 9%, respectively, while fungal population density in feed treated with the propionic acid was lower than in calcium propionate-treated feed and control. Aspergillus was the dominate genus among the microfloral population isolated from the feed during storage, whereas Aspergillus candidus was the most frequent species. No aflatoxin was detected in any of the feeds. It is concluded that propionic acid is a more effective fungistat than calcium propionate under local summer conditions.
Polyamines regulate gene expression by stimulating translation of histone acetyltransferase mRNAs

Polyamines regulate gene expression in Escherichia coli by translationally stimulating mRNAs encoding global transcription factors. In this study, we focused on histone acetylation, one of the mechanisms of epigenetic regulation of gene expression, to attempt to clarify the role of polyamines in the regulation of gene expression in eukaryotes. We found that activities of histone acetyltransferases in both the nucleus and cytoplasm decreased significantly in polyamine-reduced mouse mammary carcinoma FM3A cells. Although protein levels of histones H3 and H4 did not change in control and polyamine-reduced cells, acetylation of histones H3 and H4 was greatly decreased in the polyamine-reduced cells. Next, we used control and polyamine-reduced cells to identify histone acetyltransferases whose synthesis is stimulated by polyamines. We found that polyamines stimulate the translation of histone acetyltransferases GCN5 and HAT1. Accordingly, GCN5- and HAT1-catalyzed acetylation of specific lysine residues on histones H3 and H4 was stimulated by polyamines. Consistent with these findings, transcription of genes required for cell proliferation was enhanced by polyamines. These results indicate that polyamines regulate gene expression by enhancing the expression of the histone acetyltransferases GCN5 and HAT1 at the level of translation. Mechanistically, polyamines enhanced the interaction of microRNA-7648-5p (miR-7648-5p) with the 5′-UTR of GCN5 mRNA, resulting in stimulation of translation due to the destabilization of the double-stranded RNA (dsRNA) between the 5′-UTR and the ORF of GCN5 mRNA. Because HAT1 mRNA has a short 5′-UTR, polyamines may enhance initiation complex formation directly on this mRNA.
Adenovirus viral kinetics and mortality in ex-vivo T-cell depleted hematopoietic cell transplant recipients with adenovirus infection from a single center.

BACKGROUND We report on predictors of ADV viremia and correlation of ADV viral kinetics with mortality in ex-vivo T-cell depleted (TCD) HCT. METHODS TCD HCT recipients from 1/1/2012 through 9/30/2018 were prospectively monitored for ADV in the plasma through Day (D)+100 post-transplant or for 16 weeks after the onset of ADV viremia. ADV viremia was defined as ≥2 consecutive viral loads (VL) ≥1000 copies/mL through D+100. Time-averaged area under the curve (AAUC) or peak ADV VL through 16 weeks after onset of ADV viremia were explored as predictors of mortality in Cox models. RESULTS Of 586 patients (adult 81.7%), 51 (8.7%) developed ADV viremia by D+100. Age <18 years, recipient CMV seropositivity, absolute lymphocyte count <300 cells/µL at D+30, and acute graft-versus-host disease were predictors of ADV viremia in multivariate models. Fifteen. CONCLUSION In TCD HCT, peak ADV VL and ADV AAUC correlated with mortality at D+180. Our data support the potential utility of ADV viral kinetics as endpoints in clinical trials of ADV therapies.
Physical training in the fire station and firefighters' cardiovascular health.

BACKGROUND Few fire departments in Québec have a diversified health promotion programme. Yet, many allow firefighters to physically train during working hours. AIMS To compare the weekly physical activity (PA) level and cardiovascular health indicators of firefighters who physically train on duty to those who do not. METHODS Participants underwent a cardiovascular health assessment and completed an online questionnaire. RESULTS One hundred and five full-time male firefighters participated in the study. Two groups were formed: firefighters who physically train while on duty (E, n = 64) and firefighters who do not (NoE, n = 41). Following statistical adjustments, off-duty weekly PA was not different between the two groups (E: 239 ± 224 versus NoE: 269 ± 249 min, P = 0.496); however, total weekly PA was higher (P = 0.035) in E (381 ± 288 min) than in NoE (274 ± 200 min). A difference was also observed in obesity prevalence measured with waist circumference (E: 9% versus NoE: 27%, P = 0.026) and in physical inactivity prevalence (E: 0% versus NoE: 27%, P < 0.001). After statistical adjustments, E firefighters have a significantly lower waist-to-height ratio than NoE firefighters (E: 0.51 ± 0.05 versus NoE: 0.54 ± 0.05, P = 0.017). CONCLUSIONS Results show that firefighters who physically train while on duty have a higher total PA level on a weekly basis and have better cardiovascular health indicators. Our findings suggest that fire services should promote physical training while on duty to improve firefighters' cardiovascular health.
Endoscopic rendezvous recanalization for complete anastomotic obstruction after retrosternal coloplasty: a novel approach through a cervicotomy

A healthy 19-year-old woman previously underwent a bipolar esophageal exclusion and total gastrectomy following caustic ingestion. After 13 months, she underwent an esophageal replacement by retrosternal left colonic interposition. She subsequently developed complete obstruction of the pharyngocolonic anastomosis. Because blinded antegrade reopening of the mucosa was considered too hazardous, we considered performing a combined antegrade–retrograde endoscopic rendezvous recanalization. Owing to the prior gastrectomy and because of the retrosternal route of the coloplasty, a left cervicotomy was considered to be the best choice for the retrograde access below the anastomosis. The procedure was performed by two endoscopists and a thoracic surgeon (▶Fig. 1; ▶Video 1). The cervical colon was mobilized through a left cervicotomy, paying particular attention not to compromise its vascular pedicle. A 2-cm colotomy was performed on the tenia coli, allowing antegrade introduction of the endoscope (▶Fig. 2). The complete obstruction was identified with the two endoscopes by transillumination. Antegrade puncture using a 19-gauge needle was directed by the retrograde endoscope. After the obstruction had been successfully punctured, a Fil-guide Hydra Jagwire (0.035 inch) was introduced through the obstruction (▶Fig. 3). Dilation up to 15mm was performed using a balloon from the antegrade side, and this was followed by insertion of a nasogastric tube to maintain the patency. The outcome was uneventful. Repeated dilations were required to achieve definitive re-sizing of the anastomosis. Treatment of cervical anastomotic obstruction after coloplasty for caustic injury is challenging [1–3]. The endoscopic antegrade–retrograde technique appears to be an effective salvage therapy in complete anastomotic obstruction [4]. The most difficult part of the rendezvous procedure is gaining access to the colon below the anastomosis. Normally, a previous percutaneous endoscopic gastrostomy (PEG) is an easy route for the retrograde endoscopy [5]. Our case shows that a cervicotomy could be a simple option to gain access to the retrograde colon below the cervical anastomosis and avoid complex re-intervention in the abdomen.
Transposable elements contribute to the genome plasticity of Ralstonia solanacearum species complex

The extensive genetic diversity of Ralstonia solanacearum , a serious soil-borne phytopathogen, has led to the concept that R. solanacearum encompasses a species complex [ R. solanacearum species complex (RSSC)]. Insertion sequences (ISs) are suggested to play an important role in the genome evolution of this pathogen. Here, we identified and analysed transposable elements (TEs), ISs and transposons, in 106 RSSC genomes and 15 Ralstonia spp. We mapped 10 259 IS elements in the complete genome of 62 representative RSSC strains and closely related Ralstonia spp. A unique set of 20 IS families was widespread across the strains, IS5 and IS3 being the most abundant. Our results showed six novel transposon sequences belonging to the Tn3 family carrying passenger genes encoding antibiotic resistance and avirulence proteins. In addition, internal rearrangement events associated with ISs were demonstrated in Ralstonia pseudosolanacearum strains. We also mapped IS elements interrupting avirulence genes, which provided evidence that ISs plays an important role in virulence evolution of RSSC. Additionally, the activity of ISs was demonstrated by transcriptome analysis and DNA hybridization in R. solanacearum isolates. Altogether, we have provided collective data of TEs in RSSC genomes, opening a new path for understanding their evolutionary impact on the genome evolution and diversity of this important plant pathogen.