Miracle-dz/UniFAR-FTUs huggingface dataset:
Internalization of Vitronectin-Thrombin-Antithrombin Complex by Endothelial Cells Leads to Deposition of the Complex into the Subendothelial Matrix (*)

Internalization of the ternary vitronectin-thrombin-antithrombin (VN-TAT) complex by human umbilical vein endothelial cells was investigated. Radiolabeled VN-TAT was bound to the cell surface at 4°C, and internalization was initiated by increasing the temperature to 37°C. After 30 min about half of the VN-TAT complex disappeared from the cell surface and accumulated in the subendothelial matrix. Translocation of VN-TAT complex from the luminal to the basolateral side was confirmed by electron microscopic evaluation of cross-sections of endothelial cells incubated with gold-conjugated VN-TAT complex. Furthermore, cells cultured in VN-TAT deficient serum, incubated with purified VN-TAT, and subsequently assayed for fluorescent staining using a monoclonal antibody directed against thrombin-modified antithrombin and a polyclonal antibody against vitronectin showed co-localization of both antibodies in punctates. Punctates were randomly distributed in both the xy and xz plane of endothelial cells as evidenced by confocal laser scanning microscopy. Trichloroacetic acid precipitation and SDS-polyacrylamide gel electrophoresis showed that VN-TAT was not degraded during translocation and inhibition of the microfilament system reduced release of VN-TAT to the matrix, indicating that transcytosis was responsible for translocation. These findings emphasize that VN-TAT complex is taken up by endothelial cells, not only leading to the removal of inactivated thrombin from the circulation but also to deposition of VN into the subendothelial matrix.
A Graph Theoretical Approach to Handicap Ranking of Tournaments and Paired Comparisons

In this paper, the players in a tournament T are ranked according to the number of out arborescences rooted at the vertices of a digraph D associated with T. This ranking is shown to be equivalent to handicap ranking of tournaments. The tournaments considered in this paper may involve any number of games or no games between particular pairs of players and thus are more general than round-robin tournaments. In fact, these tournaments can be interpreted in statistics as systems of paired comparisons.
Are interventions theory-based? Development of a theory coding scheme.

OBJECTIVE Interventions are increasingly described as theory-based; however, the basis for this is often not clear. Advancing behavioral science requires a good understanding of how interventions are informed by, and test, theory. This study aims to develop a reliable method for assessing the extent to which behavioral interventions are theory-based. DESIGN The reliability, usability, and comprehensiveness of an initial coding scheme were improved in 13 iterative stages on the basis of its application to 29 papers, from a systematic review of interventions to promote physical activity and healthy eating. RESULTS The final Theory Coding Scheme contained 19 items, each with satisfactory interrater reliabilities, coding whether a theory or model was mentioned, how theories were used in intervention design, how intervention evaluations tested theory, and the implications of the results for future theory development. CONCLUSION The Theory Coding Scheme is an important methodological innovation, providing a research tool to reliably describe the theoretical base of interventions, inform evidence syntheses within literature reviews and meta-analyses, and stimulate the use of empirical data for theory development.
Coloring Bull-Free Perfectly Contractile Graphs

We consider the class of graphs that contain no bull, no odd hole, and no antihole of length at least five. We present a new algorithm that colors optimally the vertices of every graph in this class. This algorithm is based on the existence in every such graph of an ordering of the vertices with a special property. More generally we prove, using a variant of lexicographic breadth-first search, that in every graph that contains no bull and no hole of length at least five there is a vertex that is not the middle of a chordless path on five vertices. This latter fact also generalizes known results about chordal bipartite graphs, totally balanced matrices, and strongly chordal graphs.
RELATIVE CONTRIBUTION OF QUANTITATIVE AND QUALITATIVE CHANGES IN MITOCHONDRIA TO METABOLIC COMPENSATION DURING SEASONAL ACCLIMATISATION OF RAINBOW TROUT ONCORHYNCHUS MYKISS

This study examined whether changes in the properties of mitochondria from red muscle of rainbow trout Oncorhynchus mykiss are accompanied by ultrastructural changes during cold acclimatisation. We compared measurements at five levels of organisation in red muscle of winter- (1 °C) and summer- (16 °C) acclimatised trout. We examined (1) maximal rates of pyruvate and palmitoyl carnitine oxidation by isolated mitochondria, (2) enzymatic activities [cytochrome c oxidase (CCO), citrate synthase (CS), carnitine palmitoyl transferase (CPT) and phosphofructokinase (PFK)] of the muscle and isolated mitochondria, (3) mitochondrial protein content in the muscle, (4) the ultrastructure of muscle fibres, and (5) the cristae surface density of the mitochondria. All variables were measured on each trout sampled. The mitochondria from winter-acclimatised trout possessed higher maximal capacities for the oxidation of pyruvate and palmitoyl carnitine than those from summer-acclimatised trout. Muscle activities of CCO, CS and CPT were greater in winter than in summer trout, whereas the levels of PFK did not differ seasonally. Similarly, the mitochondria from winter trout had elevated levels of CCO, CS and CPT compared with those isolated from summer trout. The cristae surface density of the mitochondria from winter trout (40.2+/-0.6 micrometre2 micrometre-3; mean +/- s.e.m.) was significantly higher than that from summer trout (36.4+/-1.2 micrometre2 micrometre-3), whereas there was no difference in the mitochondrial volume densities of muscle fibres from winter and summer trout. Thus, the considerable compensation of muscle aerobic capacity at low temperatures in trout is not accompanied by changes in mitochondrial volume density, but rather by shifts in enzyme levels and cristae surface density.
Marginal Adaptation and Microleakage comparison between two Zirconia Oxide systems with the same cement

Goal: To compare the marginal fit and micro-leakage of Lava™ and Zirkon zahn® copings using the same cement as adhesive. Materials and Methods: Twenty extracted superior premolars were divided into two groups. Teeth in the first group were prepared to receive Lava™ copings, while teeth in the second group were prepared for Zirkon zahn® copings. Copings were prepared following each system´s standard procedure. Marginal fit was measured in micrometers in eight different regions, both before and after being bound with RelyX™ U100 cement. Once thermocycled, samples were soaked in 2% fuchsin dye solution and buccolingually sliced, microleakage was measured, both in the vestibular and palatal regions. Results: A statistically significant difference in marginal fit between the two zirconia systems was found. Lava™ registered the best adaptation values: 19. 7µm before cementation and 15. 0µm after, while Zirkon zahn® reported marginal fit values of 28. 1µm (before) and 22. 8µm (after cementation). In contrast, we found no significant difference in microleakage: Lava™ averaged 314. 2µm and Zirkon zahn® 319. 8µm. Conclusion: Lava™ registered better marginal fit values than Zirkon zahn®, but no significant difference in microleakage. Key Words: Marginal fit, microleakage, zirconia, CAD-CAM.
Interaction of hydrophobic anions with the rat skeletal muscle chloride channel ClC‐1: effects on permeation and gating

1 Permeation of a range of hydrophobic anions through the rat skeletal muscle chloride channel, rClC‐1, expressed in Sf‐9 (a Spodoptera frugiperda insect cell line) cells has been studied using the whole‐cell patch‐clamp technique. 2 Bi‐ionic reversal potentials measured with external application of foreign anions gave the following permeability sequence: Cl− (1) > benzoate (0·15) > hexanoate (0·12) > butyrate (0·09) > propionate (0·047) x≈ formate (0·046). Anions with larger hydrophobic moieties were more permeant, which suggested that ClC‐1 selectivity for hydrophobic anions is dominated by their interaction with a hydrophobic region in the external mouth of the pore. 3 All anions studied when applied from outside show an apparently paradoxical voltage‐dependent block of inward currents; this voltage‐dependent block could be qualitatively described by a discrete‐state permeation model with two binding sites and three barriers. 4 Effects of the external anions with aliphatic side‐chains on the apparent open probability (Po) suggested that they are unable to gate the channel, but can modulate ClC‐1 gating, probably, by changing Cl− affinity to the gating site. 5 Effects of internal application of benzoate, hexanoate or propionate mimicked those of increasing internal pH, and similarly depended on the channel protonation from the external side. Results for internal benzoate support the concept of a negatively charged cytoplasmic particle being involved in the ClC‐1 gating mechanism sensitive to the internal pH.
The evolving role of PET/CT in breast cancer

Departments of Nuclear Medicine, Medical Oncology, Breast Diseases Unit, Saint-Louis Hospital, EAD Imagerie Moléculaire Diagnostique et Ciblage Thérapeutique, IUH, University of Paris VII, Paris, France, Department of Nuclear Medicine, Santa Maria della Misericordia, Rovigo Hospital, Rovigo, Italy and Department of Nuclear Medicine, Imperial College Healthcare, Hammersmith Hospital, London, UK Correspondence to Domenico Rubello, MD, Department of Nuclear Medicine, PET/CT Centre, Medical Physics, Radiology, Santa Maria della Misericordia Hospital, Rovigo, Italy Tel: + 39 320 7985599; fax: + 39 42 5394434; e-mail: domenico.rubello@libero.it
Ethnic Differences in Stress, Coping, and Depressive Symptoms after the Exxon Valdez Oil Spill

This study assessed levels of depressive symptomatology in a household probability sample of Alaskan Native (N = 188) and Euro-American (N = 371) residents of 13 communities in Alaska. Our objective was to examine ethnic differences in both the association between depressive symptomatology and exposure to the Exxon Valdez oil spill and subsequent cleanup efforts, and in the role of family support as a moderator of exposure to this technological disaster. Level of exposure was significantly associated with mean Center for Epidemiological Studies-Depression Scale scores in both Natives (p <.05) and Euro-Americans (p <.01). Both ethnic groups also reported significant declines in traditional relations with increasing levels of exposure (p <.001). However, Natives had a significantly higher mean Exposure Index score than Euro-Americans and were more likely to report working on cleanup activities, damage to commercial fisheries, and effects of the spill on subsistence activities. Depressive symptomatology was associated with reported participation in cleanup activities and other forms of contact with the oil in Natives, and reported damage to commercial fisheries, use of affected areas, and residence in a community in geographic proximity to the spill in Euro-Americans. Perceived family support was not directly associated with depressive symptoms in either ethnic group, but did serve to buffer the effects of exposure on depressive symptoms in Euro-Americans. The results suggest that cultural differences play an important role in determining the psychosocial impacts of a technological disaster, particularly with respect to exposure, appraisal of an event as stressful, perceived family support as a moderator of stress, and expression of depressive symptomatology.
A Integrated Power Flow Algorithm for Radial Distribution System with DGs Based on Voltage Regulating

It proposes a promising power flow analysis method for radial distribution system with distributed generations (DGs), combined with reactive power compensation facilities. In the power flow analysis, suitable models are determined for different DG units, Q-sources including Static Var Compensator, Switched Capacitor or Shunt Capacitor and Step Voltage Regulator. A helpful method is presented to deal with the PV node, static voltage control model node and PI node during the power flow iteration, which makes Backward/Forward Sweeps technique suitable to calculate radial distributed system power flow with multiple node styles. Then an example of 28-node radial distribution system with DGs is performed to validate the method proposed in this paper. In addition, the simulation results show that DGs influence on voltage profile, power losses and voltage regulator operating status greatly.
MICIE: A Model for Identifying and Collecting Intrusion Evidences

Today it is very important to maintain an intermediary level of security to ensure safe and trusted communication for daily usage. Secured data communication over internet and any other network is hard to achieve due to the threat of intrusions and misuse. Unfortunately, none of the existing systems have proved to be flawless, though various approaches have been used to thwart network intrusion activities. This paper proposes an investigation Model for Identifying and Collecting Intrusion Evidences (MICIE). In particular, the proposed model MICIE comprises three main features, SNORT as IDS, MySQL as database and BASE for result viewing. These features were installed on Raspberry Pi, which was used to aid the data collection process. The results demonstrated that the proposed model is promising for identifying and collecting evidence of network intrusions in real time.
Chambered cuticle, pellicles, strange sensilla, and extraordinary muscle arrangements: A study of the micropterigid larval trunk (Lepidoptera: Micropterigidae)

The larvsal trunk wall of Sabatinca chalcophanes (Meyrick, 1885), representing the “sabatincoid morphotype,” is described (brightfield and polarization microscopies, scanning and transmission electron microscopies). Eight sensillum types are identified, including four previously undescribed subventral and ventral kinds. The cuticle is nonsolid, the exocuticle being chambered in a honeycomb‐like fashion with chamber walls apparently secreted along epidermal cells boundaries. The chamber contents open to the exterior via minute pores in the chamber roofs. A space between endo and exocuticle communicates with the chamber interiors via pores in the chamber floors; the dense endocuticular surface in places form thickened domes. On the lower trunk region, lateral chamber walls are highly porous (lattice like), hence their contents are continuous; individual chamber roofs here are markedly convex, and the external trunk surface, therefore, papillate. The trunk surface is more or less completely covered by a pellicle, likely formed by exudates from the exocuticular chambers. Unusually for Lepidoptera all trunk muscles are slender strands covering a modest proportion of the inner trunk surface. Conspicuous insertion “nodes” are located at lateral and ventral segmental boundaries, ventromedially near segmental midlengths, and paramedially on the dorsum behind segmental midlengths. Overall similar cuticular specializations are also present in the distantly related Micropterix, strongly supporting micropterigid monophyly. J. Morphol. 275:797–821, 2014. © 2014 Wiley Periodicals, Inc.
Two-Photon Polymerization: A New Approach to Micromachining Femtosecond lasers enable microfabrication with resolution beyond the diffraction limit.

ment of ultrashort laser systems is creating exciting possibilities for very precise localization of laser energy in time and space. These achievements have triggered novel laser applications based on nonlinear interaction processes. A promising three-dimensional microfabrication method that has recently attracted considerable attention is based on twophoton polymerization with ultrashort laser pulses.1-5 When focused into the volume of a photosensitive material (or photoresist), the pulses initiate two-photon polymerization via two-photon absorption and subsequent polymerization. After illumination of the desired structures inside the photoresist volume and subsequent development — e.g., washing out the nonilluminated regions — the polymerized material remains in the prescribed 3-D form. This allows fabrication of any computer-generated 3-D structure by direct laser “recording” into the volume of a photosensitive material (Figure 1). Because of the threshold behavior and nonlinear nature of the process, a resolution beyond the diffraction limit can be realized by controlling the laser pulse energy and the number of applied pulses. As a result, the technique provides much better structural resolution and quality than the
Modeling Lorentz dispersive media in FDTD using the exponential time differencing method

In this paper, we present the formulation of update equations for modeling electromagnetic wave propagation in second-order pole Lorentz media using the ETD method. As will be shown later, the formulation will result in less memory storage requirement compared to the ADE method. Numerical results also indicate that the ETD method is more accurate compared with other methods such as the ADE method and PLRC method.
Defining a Lingua Franca to Open the Black Box of a Naïve Bayes Recommender

Many AI systems have a black box nature that makes it difficult to understand how they make their recommendations. This can be unsettling, as the designer cannot be certain how the system will respond to novelty. To penetrate our Na\"ive Bayes recommender's black box, we first asked, what do we want to know from our system, and how can it be obtained? The answers led us to recursively define a common lexicon with the AI, a lingua franca, using the very items that the system ranks to create meta-symbols recognized by the system, and enabling us to understand the system's knowledge in plain terms and at different levels of abstraction. As one bonus, using its existing knowledge, the lingua franca can enable the system to extend recommendations to related, but entirely new areas, ameliorating the cold start problem. We also supplement the lingua franca with techniques for visualizing the system's knowledge state, develop metrics for evaluating the meaningfulness of terms in the lingua franca, and generalize the requirements for developing a similar lingua franca in other applications.
Chirp-Compensated DBR Lasers for TWDM-PON Applications

We propose a novel chirp compensation scheme for directly modulated three-section distributed Bragg reflector (3s-DBR) lasers in applications of the time- and wavelength-division multiplexed passive optical network systems. The frequency chirp of 3s-DBR lasers induced by direct modulation is investigated through extensive numerical simulations based on the time-domain travel-wave model. To overcome the signal distortions resulting from the frequency chirp due to direct modulation, we extend on our novel idea of frequency chirp compensation technology with inherent characteristics of DBR-type tunable lasers. Q factors and extinction ratio for different system transmission lengths are analyzed and compared. Large dispersion tolerance of the system is achieved by using the chirp compensation scheme.
Changes in the permeability of Escherichia coli during parasitization by Bdellovibrio bacteriovorus.

Bdellovibrio bacteriovorus strain 6-5-S completed a typical infection cycle when incubated with E. coli ML 35 (lac i−z+y−) in 0.025 M Hepes buffer, pH 7.8, supplemented with 0.002 M CaCl2∙2H2O. Gro.
Metabolism and Elimination of Oxytetracycline in Hepatopancreas of Chinese Mitten Crab Eriocheir Sinensis

The kinetic profiles of Oxytetracycline (OTC) in hepatopancreas of Eriocheir sinensis was examined in this research. The results showed that after a single-dose of 40 mg/kg body weight OTC at (18±1°C) by intramuscular administration, the hepatopancreas concentration-time curve of OTC could be best described by a one-compartment model with first-order absorption and a time lag: C = 69.45×(e-0.005(t-0.0387)-e-8.195 (t- 0.0387)). The elimination half-life (t1/2β) was 130.71 h. It is suggested that a withdrawal time of 79.51d should be required before crabs are consumed safely.
POSTCRANIAL ANATOMY OF VIVERRAVUS (MAMMALIA, CARNIVORA) AND IMPLICATIONS FOR SUBSTRATE USE IN BASAL CARNIVORA

Abstract A postcranial skeleton of the viverravid carnivoran, Viverravus acutus from the early Eocene of Wyoming, is described and compared to contemporary carnivorans (the viverravid Didymictis, and the miacids Miacis and Vulpavus), and to extant taxa belonging to the families Mustelidae, Procyonidae, Canidae, Viverridae, and Herpestidae. Based on humeral and femoral midshaft diameters, body mass for this animal is estimated to have been about 250 g, less than all but a few living species of Carnivora. Shoulder and hip morphology indicate a considerable range of motion and the structure of the humeroulnar joint suggests habitually flexed postures, characteristics typical of extant carnivorans that are excellent climbers. These similarities are also shared with miacids, supporting the hypothesis that basal members of the order Carnivora were well adapted for exploiting arboreal habitats. An astragalus tentatively attributed to the middle Eocene species Viverravus gracilis, however, is similar to that of Didymictis and suggests a greater emphasis on terrestrial locomotion than is found in miacids.
The Metabolic Syndrome and Cardiometabolic Risk: Scope of the Problem and Current Standard of Care

The metabolic syndrome is a public health concern because its prevalence is increasing in the United States, and it is associated with cardiovascular morbidity and mortality. We discuss epidemiologic trends in and causes of the metabolic syndrome. The relationships among the cardiometabolic risk factors that contribute to the metabolic syndrome—obesity, insulin resistance, proinflammatory and prothrombotic states, atherogenic dyslipidemia, and hypertension—and between these risk factors and cardiovascular morbidity and mortality are explained. Currently available interventions to modify these risk factors and the importance of preventive strategies and early detection efforts are also addressed.
A Three-Stage Optimization Algorithm for the Stochastic Parallel Machine Scheduling Problem with Adjustable Production Rates

We consider a parallel machine scheduling problem with random processing/setup times and adjustable production rates. The objective functions to be minimized consist of two parts; the first part is related with the due date performance (i.e., the tardiness of the jobs), while the second part is related with the setting of machine speeds. Therefore, the decision variables include both the production schedule (sequences of jobs) and the production rate of each machine. The optimization process, however, is significantly complicated by the stochastic factors in the manufacturing system. To address the difficulty, a simulation-based three-stage optimization framework is presented in this paper for high-quality robust solutions to the integrated scheduling problem. The first stage (crude optimization) is featured by the ordinal optimization theory, the second stage (finer optimization) is implemented with a metaheuristic called differential evolution, and the third stage (fine-tuning) is characterized by a perturbation-based local search. Finally, computational experiments are conducted to verify the effectiveness of the proposed approach. Sensitivity analysis and practical implications are also discussed.