Miracle-dz/UniFAR-FTUs huggingface dataset:
Inside-out Integrin Signaling in Macrophages ANALYSIS OF THE ROLE OF THE AND a6BP1 INTEGRIN VARIANTS IN LAMININ ADHESION cDNA EXPRESSION IN AN a6 INTEGRIN-DEFICIENT MACROPHAGE CELL LINE*

Leukocytes use the a681 integrin to adhere to lami- nin based on mAb inhibition and affinity chromatog-raphy studies. This adhesion requires leukocyte stim- ulation with either PMA or specific cytokines, a process that has been termed “inside-out’’ integrin signaling. In the present study, the involvement of a6 integrin structural variants in this regulated adhesion was examined using mouse macrophages. The two known a6 structural variants, a6A and a6B, differ only in their cytoplasmic domain sequences. Using re- verse transcriptase-polymerase chain reaction, we observed that macrophages express only the a6A structural variant, in contrast to most cell types which express both a6A and a6B variants. The role of this integrin subunit in macrophage adhesion was assessed by cDNA transfection of P388D1 cells. We found that this mouse macrophage cell line does not adhere to laminin even in response to phorbol 12-myristate 13-acetate (PMA) stimulation, though it does adhere nor- mally to fibronectin and tissue culture plastic. Subsequent analysis employing reverse transcriptase-po-lymerase chain reaction and immunoprecipitation of surface labeled cells revealed that this
Lean Six Sigma implementation: modelling the interaction among the enablers

Abstract Lean Six Sigma (LSS) methodology has been acquiring a prominent position in organisations. The aim of this study is to demonstrate an approach to LSS implementation in organisations using the development of a hypothetical model based on interpretive structural modelling (ISM) and fuzzy Matriced Impacts Croisés Multiplication Appliquée á un Classement (fuzzy MICMAC) analysis phenomenon. Seventy Lean Six Sigma enablers (LSSEs) have been identified through extensive literature review and out of which 40 most important LSSEs were finalised through opinions of experts both from industry and academia. Furthermore, the valuable expert opinions have been applied to determine contextual relationships between these significant LSSEs and a hierarchical model has been created based on an ISM. The fuzzy MICMAC analysis has also been utilised to classify the enablers based on the dependence and driving power, and validate the created ISM-based model. The developed hierarchical model will assist to understand interrelationships and interdependencies among the identified LSSEs. Having high driving and low dependence power, the LSSEs have strategic significance because of their driving character. On the other hand, having high dependence and low driving power, LSSEs are more performance orientated. The mutual influence, driving and dependence power of LSSEs render valuable information to top management to distinguish between independent and dependent LSSEs. An organisation desiring of adopting LSS may get benefited by the understanding of LSSEs and their interactions.
Immunization Requires Langerhans Cells Tumor Immunotherapy by Epicutaneous

A role for Langerhans cells (LC) in the induction of immune responses in the skin has yet to be conclusively demonstrated. We used skin immunization with OVA protein to induce immune responses against OVA-expressing melanoma cells. Mice injected with OVA-speciﬁc CD8 (cid:1) T cells and immunized with OVA onto barrier-disrupted skin had increased numbers of CD8 (cid:1) T cells in the blood that produced IFN- (cid:2) and killed target cells. These mice generated accelerated cytotoxic responses after secondary immunization with OVA. Prophylactic or therapeutic immunization with OVA onto barrier-disrupted skin inhibited the growth of B16.OVA tumors. LC played a critical role in the immunization process because depletion of LC at the time of skin immunization dramatically reduced the tumor-protective effect. The topically applied Ag was presented by skin-derived LC in draining lymph nodes to CD8 (cid:1) T cells. Thus, targeting of tumor Ags to LC in vivo is an effective strategy for tumor immunotherapy. The Journal of Immunology, 2008, 180: 1991–1998.
g to Secrete IFN-Infection through their Ability pneumoniae Chlamydia Sufficient for Protection against Cells Are Each + or CD8 + Macrophages, CD4

By using a T, B, or NK cell-deﬁcient mouse strain (recombinase-activating gene (RAG)-1 (cid:3) / (cid:3) /common cytokine receptor (cid:2) -chain ( (cid:2) C R)), and T and B cell and IFN- (cid:2) -deﬁcient (RAG-1 (cid:3) / (cid:3) /IFN- (cid:2) (cid:3) / (cid:3) ) mice, we have studied the generation of immunity against infection by Chlamydia pneumoniae . We found that IFN- (cid:2) secreted by innate-cell populations protect against C. pneumoniae infection. However, NK cells were not needed for such IFN- (cid:2) -dependent innate immune protection. Inoculation of wild type, but not IFN- (cid:2) (cid:3) / (cid:3) bone marrow-derived macrophages protected RAG-1 (cid:3) / (cid:3) /IFN- (cid:2) (cid:3) / (cid:3) mice against C. pneumoniae infection. In line, pulmonary macrophages from RAG-1 (cid:3) / (cid:3) C. pneumoniae -infected mice expressed IFN- (cid:2) mRNA. Reconstitution of RAG-1 (cid:3) / (cid:3) / (cid:2) c R (cid:3) / (cid:3) or RAG-1 (cid:3) / (cid:3) /IFN- (cid:2) (cid:3) / (cid:3) mice with CD4 (cid:1) or CD8 (cid:1) cells by i.v. transfer of FACS sorted wild type spleen cells (SC) increased resistance to C. pneumoniae infection. On the contrary, no protection was observed upon transfer of IFN- (cid:2) (cid:3) / (cid:3) CD4 (cid:1) or IFN- (cid:2) (cid:3) / (cid:3) CD8 (cid:1) SC. T cell-dependent protection against C. pneumoniae was weaker when IFN- (cid:2) R (cid:3) / (cid:3) CD4 (cid:1) or IFN- (cid:2) R (cid:3) / (cid:3) CD8 (cid:1) SC were inoculated into RAG-1 (cid:3) / (cid:3) /IFN- (cid:2) (cid:3) / (cid:3) mice. Thus both nonlymphoid and T cell-derived IFN- (cid:2) can play a central and complementary role in protection against C. pneumoniae . IFN- (cid:2) secreted by nonlymphoid cells was not required for T cell-mediated protection against C. pneumoniae ; however, IFN- (cid:2) regulated T cell protective functions. The Journal of Immunology, 2004, 172: 2407–2415.
Pain Managment in Adult Palliative Patients

There are many campaigns in the world aimed at fight against the pain and public awareness and healthcare professionals about the problem of adult pain in the palliative patient and the possibilities of its better quality with the least possible side effects. The World Day of against Pain marks October 17, and each year is dedicated to a certain type of pain, proclaimed by the International association for study of pain (IASP). The 2019 was declared the global year against of pain in vulnerable populations divided into major groups: older adults, children and infants, persons with intellectual disabilities, survivors of torture and persons requiring palliative care. As human life is prolonged, an increasing number of people will suffer from more comorbidities, weaknesses and chronic diseases and conditions such as kidney, heart or lung failure. It is estimated that by 2050, population older than 60 years will be doubled. In addition, older people can experience significant psychosocial stress due to dependence on someone else’s help. Palliative care aims to preserve or improve the quality of life and alleviate the suffering through early identification, detailed assessment and treatment of symptoms. In older people, it is necessary to combine geriatric medicine and palliative care with an emphasis on comprehensive assessment, integrating social, spiritual and environmental factors. In palliative care, we take into account the concept of ‘complete pain’ which explores psychological difficulties that may affect the perception of pain and suffering.
Bone Microarchitecture in Obese Postmenopausal Chinese Women: The Chinese Vertebral Osteoporosis Study (ChiVOS)

Background Obesity is associated with improved bone mass and microarchitecture in Caucasian individuals, but evidence in obese Asian individuals is lacking. Objective To analyze the areal bone mineral density (aBMD) and bone microarchitecture in normal-weight, overweight, and obese postmenopausal Chinese women. Methods A total of 243 postmenopausal women from the Chinese Vertebral Osteoporosis Study (ChiVOS) were included and were divided into three groups (OB, obese group; OW, overweight group; NW, normal weight group) by BMI level. aBMD, trabecular bone score (TBS), and appendicular lean mass (ALM) were measured by dual‐energy X‐ray absorptiometry (DXA). Bone microarchitecture was measured by HR-pQCT at the distal radius and tibia. X-ray was performed to confirm vertebral fractures (VFs). Multiple linear regression was used to evaluate the correlations between bone parameters and ALM after adjusting for confounding variables. Results The prevalence of VFs and clinical fractures were similar among the groups. Participants in the OB group showed a lower level of osteocalcin with comparable levels of other bone turnover markers (BTMs). The aBMD at several skeletal sites was higher in the OB group than in the NW group after adjusting for age (p<0.01 for all comparisons). At the radius, the OB group had a higher Ct.Ar, Tb.vBMD, Tb.BV/TV, Tb.N, Tb.Th, and Ct.Th than the NW group after adjusting for covariates (p<0.05 for all). Differences of a similar magnitude were found at the distal tibia. There was a trend of decreasing trend in Tb.Sp, Tb.1/N/SD, and Ct.Po among groups at both sites. However, the bone microarchitecture did not differ between participants with severe obesity (BMI≥35.0kg/m2) and those with 30.0≤BMI<35 kg/m2. Multiple linear regression revealed that the associations between ALM and most of the bone microarchitecture parameters at both sites were much stronger than the association between body weight and bone parameters. Conclusion We have observed significant improvements in aBMD, bone geometry, and bone microarchitecture in obese postmenopausal Chinese women. Except for a lower level of osteocalcin in the OB group, no significant differences in BTMs were found among the groups. Compared with body weight, ALM may explain greater variance in the improvement of bone microarchitecture parameters.
Relationship Between Foreign Direct Investment, Industrial Structure Optimization, and Green Full-Factor Productivity: Empirical Evidence From Changjiang Economic Area

By setting up an evaluation indicator system (EIS) containing bad outcomes for green full-factor productivity (GFFP), this work utilizes the super epsilon-based measure (Super EBM) model to assess the GFFPs of 11 regional-level administrative regions (regions) in the Changjiang Economic Area (CEA) from 2005 to 2019, and goes on to analyze the regional difference and spatial correlation between the regional GFFPs. On this basis, spatial measurement models were adopted to analyze how GFFP is affected by foreign direct investment (FDI), industrial structure optimization (ISO), and their cross term. The results show that: During the sample period, the GFFPs in the CEA exhibited large regional differences. Shanghai’s GFFP fell on the efficient frontier. This state was not achieved by any other region in the CEA, leaving a room for improvement. In general, most regions in the lower basin of the CEA had satisfactory GFFPs, while those in the middle and upper basin had general GFFPs. Besides, the GFFP trends were similar in the upper, middle, and lower basin of the CEA. Before 2010, the GFFPs in all three regions did not change significantly. After that year, the GFFPs in the three regions began to decline. During the sample period, the Global Moran’s I values of CEA GFFPs remained positive, and went through the test of significance in most years. Thus, the GFFPs were clustered prominently in space. Considering the results of spatial measurement models, the CEA GFFPs were significantly inhibited by FDI, and promoted by ISO; the cross term between FDI and ISO positively affected GFFP. Among the control parameters, economic growth and environment regulation clearly promote GFFP, urbanization level strongly inhibits GFFP, and energy structure does not significantly affect GFFP. The research results disclose the internal correlations between FDI and ISO in the transformation to green development: the benign interaction between FDI and regional ISO paves the way to green development for the CEA.
Calcium Phosphate/Hyaluronic Acid Composite Hydrogels for Local Antiosteoporotic Drug Delivery

Despite the bone ability of self-regeneration, large bone defects require surgical intervention. Likewise, when it comes to osteoporotic bone fractures, new approaches should be considered a supportive mechanism for the surgery. In recent years, more and more attention has been attracted to advanced drug delivery systems for local osteoporosis treatment, combining appropriate biomaterials with antiosteoporotic drugs, allowing simultaneously to regenerate the bone and locally treat the osteoporosis. Within the current research, hyaluronic acid/strontium ranelate (HA/SrRan), HA/calcium phosphate nanoparticles (HA/CaP NPs), and HA/CaP NPs/SrRan hydrogels were prepared. The effect of CaP and SrRan presence in the composites on the swelling behavior, gel fraction, molecular structure, microstructure, and SrRan and Sr2+ release, as well as in vitro cell viability was evaluated. Obtained results revealed that the route of CaP nanoparticle incorporation into the HA matrix had a significant effect on the hydrogel gel fraction, rheological properties, swelling behavior, and microstructure. Nevertheless, it had a negligible effect on the release kinetics of SrRan and Sr2+. The highest cell (3T3) viability (>80%) was observed for HA hydrogels, with and without SrRan. Moreover, the positive effect of SrRan on 3T3 cells was also demonstrated, showing a significant increase (up to 50%) in cell viability if the used concentrations of SrRan were in the range of 0.05–0.2 μg/ml.
Clinicopathological Characteristics and Molecular Phenotypes of Primary Hepatic Lymphoma

Primary hepatic lymphoma (PHL) is a rare malignant tumor, occurring in 0.016% of non-Hodgkin’s lymphoma (NHL). The common histological subtype is diffuse large B-cell lymphoma (DLBCL). Due to the rarity of tumor, clinicopathological characteristics and molecular phenotypes of PHL are limited. Seven patients with PHL (primary liver DLBCL) and 13 cases of liver involvement by DLBCL diagnosed between 2014 and 2021 in our hospital were included. The genetic features were also compared between the two groups by next-generation sequencing (NGS). Differential gene expression and pathway enrichment analysis were also performed. There were some discrepancies on presenting symptoms, pathological characteristics, laboratory data, and prognosis between PHL and DLBCL-liver groups. No same mutation was found between PHL and DLBCL-liver groups by NGS. Differential gene expression analysis discovered some up- and downregulated genes in PHL compared with the DLBCL-liver group. Upregulated genes were enriched in metabolic pathways, and downregulated genes were enriched in the HTLV-1 infection pathway. PHL is a distinct entity, with unique molecular features compared to liver involvement of systemic lymphoma. Kaplan–Meier analysis showed that the prognosis of the PHL group was better than that of the DLBCL-liver group. Understanding the clinicopathological and molecular features of PHL would help to direct clinical treatment.
Does Imagery Ability Matter for the Relationship Between Temperament and Self-Confidence in Team and Individual Sport Disciplines?

The main purpose of our study was to investigate the relationship among temperamental traits, a general tendency to use imagery, and self-confidence in sport. The specific aim was to verify if general imagery mediates the relationship between temperament and self-confidence in sport, but also with respect to individual and team sport disciplines. The study involved 144 athletes aged 19–25 years (75 men and 69 women) with different lengths of training (from 1 month to 18 years) and presented different sports levels: international (n = 12), national (n = 46), and recreational (n = 86). They also represented individual (n = 73) and team (n = 68) sports. The Imagination in Sport Questionnaire (ISQ), Trait Sports Confidence Inventory (TSCI-PL), and the emotionality, activity, shyness, and sociability (EAS) Temperament Questionnaire were all completed by the participants. Results indicate that self-confidence is explained in about 13% by temperament and general imagery, but only general imagery (b = 0.22; p < 0.05) and negative axes of the temperament—emotionality (b = –0.26; p < 0.05). The test of the indirect effect of the negative axes of the temperament on self-confidence through general imagery, conducted by Preacher and Hayes bootstrapping procedure, revealed significant mediation [b = 0.37; 95% CI = (0.09; 0.68); R2Med = 0.46] suggesting that about 46% of variance explained in self-confidence by emotionality is related to general imagery. The other 3% of variance explained in self-confidence by the positive temperament axes is related to general imagery, however, it was insignificant. The general imagery explains the relationship between emotionality and self-confidence only in individual sports athletes.
From Precision Medicine to Precision Convergence for Multilevel Resilience—The Aging Brain and Its Social Isolation

Citation: Dubé L, Silveira PP, Nielsen DE, Moore S, Paquet C, Cisneros-Franco JM, Kemp G, Knauper B, Ma Y, Khan M, Bartlett-Esquilant G, Evans AC, Fellows LK, Armony JL, Spreng RN, Nie J-Y, Brown ST, Northoff G and Bzdok D (2022) From Precision Medicine to Precision Convergence for Multilevel Resilience—The Aging Brain and Its Social Isolation. Front. Public Health 10:720117. doi: 10.3389/fpubh.2022. 720117 From Precision Medicine to Precision Convergence for Multilevel Resilience—The Aging Brain and Its Social Isolation
Progresses in Fluorescence Imaging Guidance for Bone and Soft Tissue Sarcoma Surgery

R0 surgical resection is the preferred treatment for bone and soft tissue sarcoma. However, there is still a lack of precise technology that can visualize bone and soft tissue sarcoma during surgery to assist the surgeon in judging the tumor surgical boundary. Fluorescence imaging technology has been used in the diagnosis of cancer. It is a simple and essentially safe technique that takes no additional time during the operation. Intraoperative fluorescence imaging has potential application prospects in assisting the surgeons in judging the tumor boundary and improving the accuracy of surgical resection. This review mainly starts with clinical studies, animal experimentation, and newly designed probes of intraoperative fluorescence imaging of bone and soft tissue sarcoma, to appraise the application prospects of fluorescence imaging technology in bone and soft tissue sarcoma.
Being Tired or Having Much Left Undone: The Relationship Between Fatigue and Unfinished Tasks With Affective Rumination and Vitality in Beginning Teachers

The present diary study was conducted for the purpose of bridging and integrating empirical research on the antecedents and consequences of work-related ruminative processes in the evening. Based on the control theory, unfinished tasks and fatigue in the afternoon were considered as antecedents of affective rumination, while vitality was investigated as the outcome observed in the next morning to test for cyclical processes. During a 5-day diary study (including 3 weekdays and the weekend), 74 beginning teachers completed three diary entries per day. A total of 795 diary entries were obtained. Using multilevel structural equation modeling, the study supported that both fatigue and unfinished tasks explained unique shares of variance of affective rumination in the evening at the between- and within-person levels. Furthermore, affective rumination mediated the relationship between unfinished tasks and vitality as well as fatigue and vitality. However, this only held true at the between- and not the within-person level, as neither affective rumination nor fatigue and unfinished tasks predicted the following morning’s vitality at this level. The results offer insights into the antecedents of affective rumination and add to extant research on the negative consequences of affective rumination considering vitality as an outcome.
Sustainable development in the wake of the 4th industrial revolution in Indonesia

The Existing times of fundamental technological change. The rapid advances in technology changing the way we live, work and relate to one another. The three previous industrial revolutions all created major societal change and opportunity, but today’s transformation is unique in terms of the great speed with the new ideas and technologies. Consider the unlimited possibilities of having billions of people connected by mobile devices, giving rise to unprecendented processing power, storage capabilities and knowledge access. The technologies driving the fourth industrial revolution. The potential of Indonesia’s natural wealth is remarkable, both biological and non biological natural resources. The wealth when viewed geographically, from Sabang to Merauke. With a large island and thousands of small islands that surround the nature of Indonesia. Therefore, Indonesia is an archipelagic country that has enormous natural wealth. In celebrating Indonesia’s 73 years of independence, at August 17, 2018, it is important to invites all people of Indonesia to conduct self-evaluation, why various problems such as: poverty; gap between the have and don’t; deforestry; land distruction; and the storm of industrial fourth era revolution (Industry 4.0) will creating unemployment; potential for widening economic inequality, etc. Next, the question is how to make the sustainable development in the wake of the 4th industrial revolution in Indonesia
Miltefosine and Benznidazole Combination Improve Anti-Trypanosoma cruzi In Vitro and In Vivo Efficacy

Drug repurposing and combination therapy have been proposed as cost-effective strategies to improve Chagas disease treatment. Miltefosine (MLT), a synthetic alkylphospholipid initially developed for breast cancer and repositioned for leishmaniasis, is a promising candidate against Trypanosoma cruzi infection. This study evaluates the efficacy of MLT as a monodrug and combined with benznidazole (BZ) in both in vitro and in vivo models of infection with T. cruzi (VD strain, DTU TcVI). MLT exhibited in vitro activity on amastigotes and trypomastigotes with values of IC50 = 0.51 µM (0.48 µM; 0,55 µM) and LC50 = 31.17 µM (29.56 µM; 32.87 µM), respectively. Drug interaction was studied with the fixed-ration method. The sum of the fractional inhibitory concentrations (ΣFICs) resulted in ∑FIC= 0.45 for trypomastigotes and ∑FIC= 0.71 for amastigotes, suggesting in vitro synergistic and additive effects, respectively. No cytotoxic effects on host cells were observed. MLT efficacy was also evaluated in a murine model of acute infection alone or combined with BZ. Treatment was well tolerated with few adverse effects, and all treated animals displayed significantly lower mean peak parasitemia and mortality than infected non-treated controls (p<0.05). The in vivo studies showed that MLT led to a dose-dependent parasitostatic effect as monotherapy which could be improved by combining with BZ, preventing parasitemia rebound after a stringent immunosuppression protocol. These results support MLT activity in clinically relevant stages from T. cruzi, and it is the first report of positive interaction with BZ, providing further support for evaluating combined schemes using MLT and exploring synthetic alkylphospholipids as drug candidates.