Miracle-dz/UniFAR-FTUs huggingface dataset:
One and two photon absorption of atoms and ions

One and two-photon photoabsorption of atoms and ions is the primary focus of this study, with special emphasis on photoabsorption in a laser-produced strontium plasma. The dual laser plasma (DLP) technique has been employed to carry out these studies and adapted to include an optical parametric oscillator (OPO), tuneable in the visible wavelength range (410-700 nm), to permit two-photon and two-colour photoabsorption investigations. The OPO was tuned to one and two photon resonances in Sr0 and Sr+ and the XUV photoabsorption spectrum was studied for various laser intensities and detunings from resonance. Fluorescence measurements were also made to elucidate the underlying excitation dynamics in the atom/ion after laser irradiation. Absolute photoionization cross-section measurements of the Kr-isoelectronic sequence (Rb+, Sr2+) were made at the ASTRID storage ring at the University of Aarhus, Denmark. These results were complemented by measurements made with the DLP technique. Inner-shell excitation studies in Pb2+, Bi3+ and Sr+ were also undertaken with the DLP technique. Detailed atomic structure calculations using the Cowan suite of codes, based on Hartree-Fock configuration interaction methods, were performed to generate synthetic spectra for comparison with experimental results.
THE EFFECT OF THE DELETION OF OPA 5 ON THE ABILITY OF N. GONORRHOEAE STRAIN MS11 TO TRANSCYTOSE A POLARIZED T84 EPITHELIAL CELL MONOLAYER

Title of Thesis: THE EFFECT OF THE DELETION OF OPA 5 ON THE ABILITY OF N. GONORRHOEAE STRAIN MS11 TO TRANSCYTOSE A POLARIZED T84 EPITHELIAL CELL MONOLAYER Meredith Ann Davis, Master of Science, 2004 Thesis directed by: Full Professor Daniel C. Stein Department of Cell Biology and Molecular Genetics And Associate Professor Wenxia Song Department of Cell Biology and Molecular Genetics Neisseria gonorrhoeae can express 11 different opacity proteins (Opa). Plasmids were constructed that allowed for the generation of strains containing deletions in each Opa-encoding-gene (amplified by PCR and cloned into pUC19). The Opa-encoding region was replaced with a spectinomycin resistance cassette and transformed into N. gonorrhoeae. The biological properties of one of these transformants (MS11 Opa5 delta s) was tested in a transcytosis assay. Wildtype MS11, was able to efficiently cross a polarized epithelial monolayer of T84 cells within 6 hours and occurred in both the apical to basolateral and the basolateral to apical directions. The number of cells that crossed the monolayer depended upon the dose of the inoculum. Transcytosis of MS11 Opa5 delta s occurred in the apical to basolateral direction, but was delayed. The data indicate that gonococci use multiple mechanisms of transcytosis, and the Opa 5 protein is important for rapid apical to basolateral transcytosis. THE EFFECT OF THE DELETION OF OPA 5 ON THE ABILITY OF N. GONORRHOEAE STRAIN MS11 TO TRANSCYTOSE A POLARIZED T84 EPITHELIAL CELL MONOLAYER by Meredith Ann Davis Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park in partial fulfillment of the requirements for the degree of Master of Science 2004 Advisory Committee: Full Professor Daniel C. Stein, Chair Associate Professor Wenxia Song Associate Professor Spencer Benson
A survey of undiagnosed hypertension among market traders in Suva, Fiji Islands

Routine check and optimal control of blood pressure have been recommended to reduce the high cardiovascular disease burden in the Southern Pacific. As demographic profile in the Pacific Island countries continues to transition, the burden of cardiovascular disease is importunately affecting more younger and high stress population. In collaboration with the Healthy Setting Initiative of the Ministry of health, we conducted a survey of blood pressure among market traders in Suva City Central and Nasouri markets of Fiji Islands. Following ethical approval and informed consent, we obtained socio-demographic data, and measured anthropometric indices and blood pressure of asymptomatic adult market traders of Suva City and Nasouri market using standard procedures. Participants comprised of 189 traders (52% females); with mean body mass index of 30kg/m2, weight of 82kg and waist circumference of 101cm. Overall, 152(80%) had sub-optimal blood pressure: elevated 23(12%), stage I: 70(37%), stage II: 57(30) and hypertensive crisis 2(1%). Of these, 30(20%) reported being compliant with medication, 62(41%) non-compliant and 60(39%) were previously unaware of their blood pressure status. Percent body fat (OR: 1.07; 95% CI: 1.02-1.13) and diastolic blood pressure (OR: 1.34; 95% CI: 1.21-1.48) were independently associated with hypertension. Burden of hypertension is high (80%) among study participants, with 39% of these being undiagnosed. There are issues with suboptimal blood pressure control and non-compliance with medication among study participants. There is need for health education, routine screening and sustainable intervention to reduce this burden among this population.
The complete mitochondrial genome of golden yellow snakehead fish, Channa argus

Abstract We sequenced and characterized the complete mitochondrial genome of golden yellow snakehead fish, Channa argus. The mitogenomes contained the typical complement of 13 protein-coding genes, 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and a non-coding control region. They share the same gene arrangement pattern that was identical with most vertebrates. The entire mitochondrial DNA molecule of golden yellow snakehead fish was 16,558 bp long. All information reported in this article will be a useful source of sequence information for general molecular and evolutionary studies of the family Channidae.
Análise do risco de viés em estudos de revisão sistemática sobre a relação entre as alterações do valgo ou varo do joelho e a melhora da dor ou função nos distúrbios do joelho

A dor no joelho e a segunda desordem musculoesqueletica mais prevalente, sendo a osteoartrite (OA), a dor patelofemoral (DPF) e a lesao de ligamento cruzado anterior (LCA), as que possuem maior prevalencia e incidencia. O alinhamento do joelho no plano frontal e transverso pode predispor e/ou acelerar a evolucao da OA, DPF e lesao do LCA. Apesar do potencial beneficio biomecânico dos diversos tratamentos conservadores para melhorar o alinhamento dinâmico do membro inferior no plano frontal e transverso, ainda nao esta claro, se, de fato, corrigir o valgo e/ou varo do joelho nos pacientes com disturbios no joelho esta associado a melhora da dor e/ou funcao dos mesmos. Um importante passo para a busca desta questao e analisar o risco de vies dos ensaios clinicos que avaliaram o valgo/varo do joelho, dor e/ou funcao. Desta forma, o presente estudo tem como objetivo principal analisar o risco de vies dos estudos que avaliaram as alteracoes do valgo ou varo do joelho e a melhora da dor ou funcao de individuos com disturbios do joelho de estudos incluidos em revisao sistematica. Para tal, foi realizada uma analise do risco de vies dos estudos incluidos em revisao sistematica. A revisao sistematica foi conduzida de acordo com as recomendacoes do Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). As buscas eletronicas foram conduzidas em cinco bancos de dados: Medline e EMBASE via Ovid, CINAHL e SPORTDiscus via EBSCO e Web of Science. Dois revisores independentes realizaram a selecao e um terceiro pesquisador foi consultado quando houve discordância A extracao e analise dos dados foram conduzidas por dois pesquisadores independentes. A avaliacao do risco de vies dos estudos incluidos foi realizada atraves da ferramenta Revised Cochrane risk-of-bias tool for randomized trials (RoB 2). Dos 20 estudos incluidos, 15% (n=3) foram considerados de baixo risco de vies, 35% (n=7) apresentaram pontos metodologico(processo de randomizacao, desvios das intervencoes pretendida, mensuracao do resultado e selecao do resultado reportado) que deixam preocupacoes quanto ao risco de vies e 50% (n=10) foram considerados de alto risco de vies. Os dominios relacionados a mensuracao do resultado (Dominio 4), desvios das intervencoes pretendidas (Dominio 2) e selecao do resultado reportado (Dominio 5) apresentaram a maior porcentagem de alto risco de vies. Para diminuicao do risco de vies, evitando a subestimacao ou superestimacao do verdadeiro efeito de intervencao, sugere-se que os estudos futuros utilizem avaliadores, terapeutas e participantes cegos quanto a intervencao recebida pelos participantes, realizem a analise de intencao de tratar, e reportem o plano de analise previo dos dados.
Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

Spindle microtubules, whose dynamics vary over time and at different locations, cooperatively drive chromosome segregation. Measurements of microtubule dynamics and spindle ultrastructure can provide insight into the behaviors of microtubules, helping elucidate the mechanism of chromosome segregation. Much work has focused on the dynamics and organization of kinetochore microtubules, i.e. on the region between chromosomes and poles. In comparison, microtubules in the central spindle region, between segregating chromosomes, have been less thoroughly characterized. Here, we report measurements of the movement of central spindle microtubules during chromosome segregation in human mitotic spindles, and Caenorhabditis elegans mitotic and female meiotic spindles. We found that these central spindle microtubules slide apart at the same speed as chromosomes, even as chromosomes move towards spindle poles. In these systems, damaging central spindle microtubules by laser ablation caused an immediate and complete cessation of chromosome motion, suggesting a strong coupling between central spindle microtubules and chromosomes. Electron tomographic reconstruction revealed that the analyzed anaphase spindles all contain microtubules with both ends between segregating chromosomes. Our results provide new dynamical, functional, and ultrastructural characterizations of central spindle microtubules during chromosome segregation in diverse spindles, and suggest that central spindle microtubules and chromosomes are strongly coupled in anaphase.
Expression of CD133 and Aldehyde Dehydrogenase 1A1 in Borderline Ovarian Tumor and Their Correlation with International Federation of Gynecology and Obstetrics

BACKGROUND: Borderline ovarian tumor (BOT) is an epithelial ovarian tumor with atypical epithelial proliferation without stromal invasion. BOTs may have an aggressive fashion with associated microinvasion feature, peritoneal “implants,” regional lymphadenopathy, and higher International Federation of Gynecology and Obstetrics (FIGO) stage. CD133 is cancer stem cells (CSCs) marker that promotes cell proliferation and tumor invasion through induction of nuclear factor kappa B and upregulation of MMP9. Aldehyde dehydrogenase 1A1 (ALDH1A1) is CSCs marker that promotes cell proliferation through the upregulation of c-MYC and cyclin-D1. AIM: The aim of this study was to investigate the correlation between CD133 and ALDH1A1 expression with FIGO stage. This research was performed as an analytic-observational with a cross-sectional design. METHODS: This research using a paraffin block of patients diagnosed as BOT in Hasan Sadikin Hospital Bandung. Samples were divided in two groups: FIGO stage IA and FIGO stage >IA. All samples were stained by immunohistochemistry CD133 and ALDH1A1. All data were analyzed using the Chi-square test with a significant level 5%. RESULTS: This study showed a statistically significant correlation between CD133 (p = 0.047) and ALDH1A1 (p = 0.042) expression with FIGO stage in BOT. Multivariate analysis showed there was no correlation between CD133 and ALDH1A1 in the affected of FIGO stage in BOT. CONCLUSION: CD133 and ALDH1A1 in BOT can be considered as a factor to predict the prognosis of BOT through predict FIGO stage.
Challenges in Improving the Services of La Union Electric Cooperative Incorporated (LUELCO, Inc.) For Member-Consumer-Owners’ (MCOS) Satisfaction In The Province of La Union

The study used descriptive-correlational research methodologies because it described the satisfaction delivered by LUELCO employees and established the relationship of the challenges encountered by a registered consumer in the field of electrification. The total sampling comprised 120 Member-Consumer-Owners (MCOs) from the six (6) service branches of LUELCO in the Province of La Union and they were taken randomly. The study had a hundred percent retrieval rate. Mean, weighted mean, rankings and Pearson Product-Moment Correlation Coefficient were used to present and interpret the results. Data revealed that the actual service (4.24) experienced by Member-Consumer-Owners (MCOs) was less than what they expected (4.60) from LUELCO which resulted in gap scores in the service dimensions of each branch. SERVQUAL RATER confirmed that Branch F (-1.06) obtained the highest gap. Noted some indicators such as consumers' confidence in employees (+0.40), consumers' best interests at heart (+0.25) and personal attention by employees (+0.20) can increase satisfaction ratings from MCOs. Recommendations were provided such as increase tangibles for Branches A, E and F for branch aesthetics while enhances service contact for Branches B, C and D. The computed Pearson’s r (0.41) confirmed that the satisfaction rating of MCOs was moderately affected by the challenges. Therefore, the study strongly suggests the creation and design of MCOs Service Flowchart and adapt the proposed plan of action.
A review on reversible logic gates

In recent years, reversible logic circuits have applications in the emerging field of digital signal processing, optical information processing, quantum computing and nano technology. Reversibility plays an important role when computations with minimal energy dissipation are considered. The main purpose of designing reversible logic is to decrease the number of reversible gates, garbage outputs, constant inputs, quantum cost, area, power, delay and hardware complexity of the reversible circuits. This paper reveals a comparative review on various reversible logic gates. This paper provides some reversible logic gates, which can be used in designing more complex systems having reversible circuits and can execute more complicated operations using quantum computers. Future digital technology will use reversible logic gates in order to reduce the power consumption and propagation delay as it effectively provides negligible loss of information in the circuit. Keywords: Garbage output, Power dissipation, quantum cost, Reversible Gate, Reversible logic,
Experimental and theoretical investigation of drotaverine binding to bovine serum albumin

This study was motivated by the need to provide more insight into the possible mechanism of the intermolecular interactions between antispasmodic drug drotaverine and one of the serum albumins (BSA), with the aim to indicate the most probable sites of these interactions. For this purpose both experimental (spectrofluorometric titration at various temperatures) and theoretical (molecular mechanics) methods have been applied. The obtained results clearly showed that drotaverine quenched BSA fluorescence, and the most probable mechanism is static quenching. The negative value of the theoretically predicted binding free Gibbs energy (-23.8 kJ/mol) confirmed the existence of the intermolecular interactions involving drotaverine and one tryptophan within BSA protein and was well agreed with the experimentally determined value of -25.2 kJ/mol.
Molecular identification and characterization of binucleate Rhizoctonia spp. associated with black root rot of strawberry in Serbia

Mira Vojvodić1, Brankica Tanović2, Milica Mihajlović2*, Petar Mitrović3, Ivana Vico1 and Aleksandra Bulajić1 1University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade, Serbia 2Institute of Pesticides and Environmental Protection, Banatska 31b, Belgrade, Serbia 3Institute of Field and Vegetable Crops, Maksima Gorkog 30, Novi Sad, Serbia * Corresponding author: diplagronom@gmail.com Received: 29 June 2018 Accepted: 9 July 2018
Determining regional scale carbon losses from tropical peatlands using ISBAS-InSAR

<p>80% of peatlands in Indonesia and Malaysia (15% of Earth's soil carbon) are now drained for production of pulp wood and palm oil. Associated increased peat decomposition and large-scale forest fires are now significant contributors to global greenhouse gas emissions. However, carbon losses from these processes and the impact of peatland drainage remain poorly quantified across SE Asia because of the challenging scale and inaccessibility of dense tropical peatland forests. </p><p>Space-based platforms offer the opportunity for regular and efficient pan-regional monitoring and overcome inaccessibility of tropical peatland environments. A development in satellite&#160;interferometric synthetic aperture radar (InSAR) in monitoring surface motion has the potential to solve this problem. A new &#8216;intermittent small baseline subset&#8217;&#160;(ISBAS) modelling technique provides excellent coverage across almost all land surfaces irrespective of ground cover, enabling long-term measurement of peatland surface motion across whole catchments, regions and countries. Importantly, the ISBAS technique is able to determine surface deformation under tropical forest canopy using C-band&#160;InSAR, enabling continuous&#160;monitoring of surface motion ranging from 0.1&#8211;40cm/yr&#160;at spatial scales&#160;&#8805;90x90m. </p><p>This project aims to determine whether rate of subsidence of the peat surface measured by&#160;ISBAS-InSAR&#160;is a proxy for rate of carbon loss in&#160;tropical peatlands in SE Asia. ISBAS-InSAR&#160;time series and field measurements of subsidence&#160;will be used to monitor and characterise seasonal tropical peat surface oscillations over time and field radar experiments will investigate C-band radar signal attenuation with the peat surface at different moisture contents. </p>
Robotic single site sentinel lymph node fluorescence detection

In the last decades, new technologies such as robotics, single-site surgery, and near infrared fluorescence sentinel lymph node mapping has reduced invasiveness in the surgical management of oncological cancer patients. We present our surgical technique to treat low risk endometrial cancer with total hysterectomy and fluorescence sentinel lymph node mapping using robotic single site approach. The Da Vinci Si System Single port platform, with 3D 8.5 mm firefly endoscope, semi rigid monopolar hook, bipolar forceps and wristed needle holder, has been used. Robotic single-site approach with sentinel lymph node fluorescence detection is feasible and applicable for the treatment of low-risk endometrial cancer.
Spongiosarcinopsis terrestris gen. et sp. nov. (Chlorophyta, Chlorophyceae): a new genus of green algae from gray forest soil, Russia

The diversity of soil green microalgae (Chlorophyta) is generally poorly known and likely underestimated because of their morphological poverty and crypticity. The use of molecular tools has revolutionized algal diversity research, including the discovery of numerous new taxa. On the basis of light microscopy, we isolated Chlorochytrium-like strain ACSSI 023 from gray forest soil (Moscow region, Russia), but with spongy rather than network chloroplast. This strain was evaluated by means of 18S rRNA gene and ITS2 sequences analyses and ultrastructural observations. Morphology, comparing the ITS2 secondary structure, compensatory base changes, intron lacking and percent similarity of the 18S rRNA gene of the isolated strain allowed us to propose a new genus and species for it, Spongiosarcinopsis terrestris gen. et sp. nov. The genus forms a separate independent branch within the Stephanosphaerinia clade (Chlorophyta, Chlorophyceae) with type species—Spongiosarcinopsis terrestris ACSSI 023.
Sprint Specificity of Isolated Hamstring-Strengthening Exercises in Terms of Muscle Activity and Force Production

To train hamstring muscle specifically to sprint, strengthening programs should target exercises associated with horizontal force production and high levels of hamstring activity. Therefore, the objectives of this study were to analyze the correlation between force production capacities during sprinting and hamstring strengthening exercises, and to compare hamstring muscle activity during sprinting and these exercises. Fourteen track and field regional level athletes performed two maximal 50-m sprints and six strengthening exercises: Nordic hamstring exercises without and with hip flexion, Upright-hip-extension in isometric and concentric modalities, Standing kick, and Slide-leg-bridge. The sprinting horizontal force production capacity at low (F0) and high (V0) speeds was computed from running velocity data. Hamstring muscle performances were assessed directly or indirectly during isolated exercises. Hamstring muscle electromyographic activity was recorded during all tasks. Our results demonstrate substantially large to very large correlations between V0 and performances in the Upright-hip-extension in isometric (rs = 0.56; p = 0.040), Nordic hamstring exercise without hip flexion (rs = 0.66; p = 0.012) and with 90° hip flexion (rs = 0.73; p = 0.003), and between F0 and Upright-hip-extension in isometric (rs = 0.60; p = 0.028) and the Nordic hamstring exercise without hip flexion (rs = 0.59; p = 0.030). However, none of the test exercises activated hamstring muscles more than an average of 60% of the maximal activation during top-speed sprinting. In conclusion, training programs aiming to be sprint-specific in terms of horizontal force production could include exercises such as the Upright-hip-extension and the Nordic hamstring exercise, in addition to maximal sprinting activity, which is the only exercise leading to high levels of hamstring muscle activity.
Planeación de la expansión de la red de distribución considerando incertidumbre en la demanda y recursos energéticos distribuidos

Este documento contiene una metodologia para el planeamiento de las redes de distribucion de energia electrica considerando incertidumbre en la demanda y recursos energeticos distribuidos. En primer lugar, se plantea un modelo de caracterizacion horaria de la demanda que tiene en cuenta el comportamiento de la generacion distribuida, programas de respuesta de la demanda, almacenamiento de energia y conexion de vehiculos electricos. Posteriormente, se plantea la metodologia de planeamiento basada en tres principios, optimizar el uso de la infraestructura, aplazar inversiones en la red y por ultimo expandir la red cuando sea necesario. Se formula el problema de planeamiento y es solucionado utilizando un algoritmo hibrido GA-PSO. Finalmente, se presentan los resultados al aplicar la metodologia en un sistema de prueba IEEE de 33 nodos radial, obteniendo reducciones de entre un 30 y 40% respecto a la expansion con alternativas tradicionales; ademas, se realiza un analisis de diferentes escenarios de penetracion de recursos energeticos distribuidos y alternativas de expansion de la red.