Miracle-dz/UniFAR-FTUs huggingface dataset:
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae)

En la region pampeana, provincia de Buenos Aires, Argentina, la horticultura es una de las principalesactividades agricolas. Los plaguicidas biologicos disponibles en el mercado son escasos en el pais e inexistentes en base a nematodos. En este trabajo, se caracterizo un nematodo entomopatogeno nativo aislado de la pampa y se evaluo su infectividad y capacidad reproductiva en el insecto de las fresas, Lobiopa insularis. El analisis morfologico y geneticomolecular determino este aislado como el nematodo Heterorhabditis bacteriophora, designado cepa-VEli. Se aislo un fragmento del ADN genomico de 901 pb (acceso GenBank No KJ575524). La nueva cepa fue patogena contra L. insularis. En recipientes de 200 ml con suelo arcilloso autoclavado (100 ml), a concentraciones de 1.000; 5.000 y 10.000 JIs, la mortalidad en larvas vario de 25 a 77 %. A una concentracion de 10,000 JIs, la infeccion fue mayor en larvas y pupas (77 ± 20 % y 64 ± 25 %, respectivamente) que en adultos (2,2 ± 1,1 %). La concentracion letal requerida para matar 50 y 90 % de las larvas (CL50 y CL90) despues de 10 dias fue 2.840 y 23.743 IJs. Se observaron diferencias en los niveles de parasitismo y en el tiempo de emergencia de los JIs entre concentraciones y estadios aunque no en la produccion. Heterorhabditis bacteriophora VEli es el primer nematodo entomopatogeno que infecta y mata L. insularis, capaz de completar su ciclo de vida en todas las etapas del escarabajo de la savia de la fresa. Esta disponibilidad permitiria el establecimiento y posterior dispersion de la cepa en los campos de fresa. Por ultimo, este informe constituye la primera presentacion de datos sobre la caracterizacion morfologica, morfometrica y genetica molecular de una cepa nativa de nematodos entomopatogenos para la provincia de Buenos Aires, Argentina.
The hidden story of violence: Children’s experience of bullying in school in Indonesia

My research aims to unravel the complexities of bullying in school situated by conservative values and norms. This research also attempts to answer scholarships’ concerns about the lack of empirical and theoretical evidence of bullying. Qualitative by nature, this research can be identified as an autoethnography research which adopts participatory observation as the main tool to assemble the data. My research finds some practices of bullying, includes bullying based on non-conforming gender performance amongst students based on the hidden curriculum taught by the teachers sustained through a governmentality model. Moreover, my research also finds the importance of an intersectionality lens to spill out different types of bullying happened in single person identity at the same time. By referring to scholars works about students’ role in bullying, my research claims students’ role in bullying settings is very fluid based on the context, such as, situations and forms of bullying. Lastly, my research finds the absence of students’ voice in policies about bullying may affect misdirection solutions to bullying practice. My research suggests for further research in examining sustained bullying practice in school by using different ways, such as, longitudinal study. Lastly, my research recommends policy makers to review every discriminating policy in public educational institution as it indirectly impacts the practice of bullying in school.
Defisit Neraca Perdagangan: J-Curve, Perang Dagang dan Model Salter Corden

Exchange rate fluctuations and external shocks such as the trade war are predicted to put pressure on Indonesia’s trade balance. Based on VECM analysis, the exchange rate and Indonesia’s aggregate trade balance followed the J-Curve. Additionally, the trade war had impact on Rupiah depreciation and trade balance. The study also confirms eight export-oriented industries that are resilient to shocks and classified into four behaviors: (i) industries following the J-Curve; (ii) industries following the J-Curve in the long run; (iii) industriy which are not fully confirmed J-Curve, and (iv) Rest sector. The implication of this study emphasize the development of export-oriented and labor-intensive industries (Salter-Corden model). Keywords: J Curve; exchange rate; trade war; Salter Coden Model; VECM ------------------------------------------- Pergerakan nilai tukar Rupiah dan guncangan eksternal seperti perang dagang diprediksi akan memberikan tekanan terhadap neraca perdagangan. Berdasarkan analisis VECM, nilai tukar rupiah dan neraca perdagangan Indonesia secara agregat mengikuti fenomena J-Curve. Perang dagang terbukti memengaruhi depresiasi nilai tukar Rupiah dan neraca perdagangan Indonesia. Terdapat delapan industri berorientasi ekspor yang memiliki resiliensi terhadap guncangan dan diklasifikasikan menjadi empat, yakni: (i) Kelompok industri yang mengikuti fenomena J-Curve sepenuhnya; (ii) Kelompok industri dengan fenomena J-Curve pada jangka panjang; (iii) Kelompok industri yang tidak sepenuhnya mengikuti fenomena J-Curve, dan (iv) Industri lainnya. Implikasi studi ini menekankan pengembangan industri yang berorientasi ekspor dan intensif tenaga kerja (Salter-Corden model).
Examining the Relevance of T-CRM and H-CRM for the Bank-Customer Partnership Quality and Willingness to Invest More

The current study examines the use of technology and human in Customer Relationship Management (CRM), especially in the banking industry. We analysed whether human still plays a role in the relationship in this industry. The purpose of this work is to determine the role of technology (T-CRM) and human (H-CRM) in enhancing partnership quality and customer behaviour (i.e., willingness to invest more). We collected data by distributing online questionnaires. The respondents were the customers of Bank Mandiri, Bank Central Asia (BCA), and Bank Rakyat Indonesia (BRI), who have experienced in using both technology-based and human-based banking services. A total of 161 responses were used in our data analysis. The results demonstrated that T-CRM and H-CRM had positive effects on partnership quality. It was found that partnership quality has a positive effect on willingness to invest more. This research advises that T-CRM is needed in the banking industry, but technology cannot replace the benefits of humans. Technology and people based relationship are both still needed to improve the relational quality between the service providers (banks) and their customers.
The complete chloroplast genome of the lipstick tree, Bixa Orellana (Bixaceae)

Abstract Bixa orellana is a small tree known for its red, oil-soluble pigment contained in the seed coat that is used as a natural dye and food coloring. In this study, we assembled and characterized the complete chloroplast genome of B. orellana as a resource for future genetic studies. With a total length of 159,825 bp, the chloroplast genome comprised of a large single-copy (LSC) region of 89,476 bp, a small single-copy (SSC) region of 19,617 bp, and two inverted repeat (IR) regions of 25,356 bp each. A total of 127 genes were predicted, consisting of 83 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. Phylogenetic analysis confirmed the position of B. orellana within the order Malvales.
High-Data-Frequency-Ratio Information Transmission Method for Fast Dynamic Response SWPIT Systems Based on DASK Modulation

Integration of power and information transmission is promising for simultaneous wireless power and information transmission (SWPIT). This article presents a differential amplitude-shift keying (DASK) modulation-based high-data-frequency-ratio information transmission method for fast dynamic response integrated SWPIT systems. Modeling and analyses of the phase-shifting modulation of power converters indicate the potential of the DASK modulation for information transmission. Operation principle of the proposed modulation is established to maintain the stability of power transmission based on the modeling. Modulator, demodulator, and communication protocol are further designed. Using the dynamic analytical model of the transmission channel, the active intersymbol interference (ISI) elimination characteristics are analyzed. Due to the ISI elimination, a high-data-frequency-ratio and fast dynamic response SWPIT is realized. Interactions between power and information transmissions are evaluated, and the results show anti-interference performances. Finally, an experimental prototype rated at 200 W is built to verify the effectiveness of the method, and the data–frequency ratio reaches 0.5 bit/Hz. Complexity, cost, and response period of the method are reduced significantly, and it can be extended to other applications universally.
Dehumanization and perceptions of immoral intergroup behavior

Members of stigmatized groups often face dehumanization. The current work investigated whether individuals may also dehumanize perpetrators of bigotry. Three main findings emerged. First, participants perceived racists as less than fully human. This finding also generalized to sexists, suggesting that the propensity to dehumanize perpetrators of bigotry is not limited to racists. Second, the extent to which participants dehumanized racists predicted the degree to which they reported that ambiguous behaviors performed by strangers reflected racial bias. Third, this association did not emerge when participants evaluated behaviors performed by their friends, suggesting that views of "racists" in general may not be linked with evaluations of behaviors performed by at least some close others. These findings demonstrate that dehumanization extends to perpetrators of bias and predicts evaluations of their behaviors.
Investigations of carbocyclisation reactions in prodiginine alkaloid biosynthesis

Prodiginine alkaloids, including streptorubin B, metacycloprodigiosin and roseophilin, are a family of red-pigmented pyrrole-containing specialised metabolites with immunosuppressive, anticancer and antimalarial properties. The final steps in streptorubin B and metacycloprodigiosn biosynthesis are oxidative carbocyclisations catalysed by Rieske oxygenases RedG and McpG respectively. These enzymes utilize the same substrate (undecylprodigiosin) to give cyclised products with different regio and stereochemistry. In order to further investigate the McpG catalysed reaction and to develop a system that could be used to probe the mechanism, mcpG was expressed in S. albus and S. coelicolor M511. Cyclic compound was observed following feeding of undecylprodigiosin to S. albus/mcpG. Since production was low, the role of McpH in forming a complex with McpG was investigated. Unfortunately, whilst McpH was shown to catalyse the condensation of 2-undeyclpyrrole (2-UP) and 4-methoxy-2, 2’ bipyrrole-5-carboxaldehyde (MBC), co-expression of mcpH with mcpG did not improve production of metacycloprodigiosin. Since whole genome sequencing showed that the mcpG expression construct originally used to prove the function of this gene contained a 5' truncation due to a sequencing error, full length mcpG was expressed in a redG mutant of S. coelicolor. This resulted in production of metacyloprodigiosin, which was confirmed by mass spectrometry and 1H and 13C NMR spectroscopy. The rph gene cluster reported to be responsible for the biosynthesis of prodigiosin R1 and roseophilin in S. griseoviridis contains four redG orthologues (rphG, rphG2, rphG3 and rphG4). Unfortunately, neither expression of the rphG genes in the redG mutant of S. coelicolor, nor the feeding of 11-methyldodecylprodigiosin to S. albus separately expressing each of the rphG genes resulted in the production of carbocylic products. Bioinformatics analysis of the S. longispororuber genome sequence revealed several cryptic natural product biosynthetic gene clusters. The likely products of some of these clusters are discussed in the light of predictive sequence analysis.
Widespread Suppression of High-Order Visual Cortex During Blinks and External Predictable Visual Interruptions

Spontaneous eye blinks generate frequent potent interruptions to the retinal input and yet go unnoticed. As such, they provide an attractive approach to the study of the neural correlates of visual awareness. Here, we tested the potential role of predictability in generating blink-related effects using fMRI. While participants attentively watched still images of faces and houses, we monitored naturally occurring spontaneous blinks and introduced three kinds of matched visual interruptions: cued voluntary blinks, self-initiated (and hence, predictable) external darkenings, and physically similar but unpredictable external darkenings. These events’ impact was inspected using fMRI across the visual hierarchy. In early visual cortex, both spontaneous and voluntary blinks, as well as predictable and unpredictable external darkenings, led to largely similar positive responses in peripheral representations. In mid- and high-level visual cortex, all predictable conditions (spontaneous blinks, voluntary blinks, and self-initiated external darkenings) were associated with signal decreases. In contrast, unpredictable darkenings were associated with signal increases. These findings suggest that general-purpose prediction-related mechanisms are involved in producing a small but widespread suppression of mid- and high-order visual regions during blinks. Such suppression may down-regulate responses to predictable transients in the human visual hierarchy.
Hard Tissue-Forming Ability and Ultra-Micro Structure of Newly Developed Sponges as Scaffolds Made with Sodium Alginate Gel and Chondroitin Sulfate

To regenerate teeth and bones, a scaffold is essential. Hydroxyapatite has been used in many studies, but scaffolds made of hydrogel or sponge are also effective. The hardness of hydroxyapatite is a disadvantageous property for shaping. A sponge is suitable as a scaffold because the shape of the scaffold can be matched to the defect. Sodium alginate (AL) has excellent biocompatibility and a sponge can be made from this gel by lyophilization. The purpose of this study was to promote hard tissue formation with a sponge made of AL gel or AL gel and chondroitin sulfate (Chs). Sponges were made from AL gel, which were then used as a scaffold to investigate their effectiveness for the formation of hard tissue or bone. Hard tissue formation in the pores of these AL sponges was estimated in vitro and in vivo. In the sponge made from AL gel, the concentration of AL and the addition of Chs affected bone formation. Concentration of AL would affect the shape and size of the pores. ALP activity in the sponges was also enhanced by Chs. The amount of osteocalcin (OC) produced in the sponge by rat bone marrow cells increased depending on the AL and Chs concentrations in the gel. The level of OC amount in the sponges made from AL gel containing Chs was notable in vivo. Bone formation in the sponge in vivo was affected by the addition of Chs in AL gel. The quantity of OC and the bone formation in AL sponges in subcutaneous tissue in vivo suggested that AL sponges can be useful as a scaffold.
The creation of sexual dimorphism in Drosophila gonad stem cell niches

Sex-specific development of the gonads is a key aspect of sexual dimorphism that is regulated by Doublesex/Mab3 Related Transcription Factors (DMRTs) in diverse animals species. We find that in mutants for Drosophila dsx, important components of the male and female gonad stem cell niches (hubs and terminal filaments/cap cells, respectively) still form. Initially, gonads in all dsx mutants (both XX and XY) initiate the male program of development, but later half of these gonads switch to form female stem cell niche structures. One individual can have both male-type and female-type gonad niches, however male and female niches are usually not observed in the same gonad, indicating that cells make a “group decision” about which program to follow. We conclude that dsx does not act in an instructive manner to regulate male vs. female niche formation, as these structures form in the absence of dsx function. Instead, dsx acts to “tip the balance” between the male or female programs, which are then executed independent of dsx. We show that bric a brac acts downstream of dsx to control the male vs. female niche decision. These results indicate that, in both flies and mammals, the sexual fate of the somatic gonad is remarkably plastic and is controlled by a combination of autonomous and non-autonomous cues.
SOLUTIONS AND TYPE CURVES OF A FLUID FLOW MODEL FOR NATURALLY FRACTURED RESERVOIRS WITH INFLUX RECHARGE

SOLUTIONS AND TYPE CURVES OF A FLUID FLOW MODEL FOR NATURALLY FRACTURED RESERVOIRS WITH INFLUX RECHARGE Luis X. Vivas-Cruz,1 Jorge Adrián Perera-Burgos,2,∗ M.A. Taneco-Hernández,3 & Alfredo González-Calderón4 1Centro de Ingeniería y Desarrollo Industrial (CIDESI), Av. Playa Pie de la Cuesta 702, Desarrollo San Pablo, Querétaro, Qro, 76125, México 2CONACYT – Unidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán A.C. , Calle 8, No. 39, Mz. 29, S.M. 64, Cancún, Quintana Roo, 77524, México 3Facultad de Matemáticas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas, Cd. Universitaria Sur, Chilpancingo, Guerrero, 39087, México 4CONACYT – CIDESI sede Campeche, Ctra. Carmen Puerto Real km 7.5, Mundo Maya, Cd. del Carmen, Campeche 24153, México
Tomato-Aphid Interactions in Plants Grown on Soil with Biochar

Plants are affected by various types of stress. The resistance or the susceptibility of plants to stress depends on the mutual characteristics of the plant and the stress. The plant can counteract the stress through the expression of specific genes, through changes in metabolism or through quantitative and qualitative variations of gene expression. Biotic stress is due to the action of viruses, bacteria and small insects and it is the cause of most of the reduction in crop yield. Biochar is a fine-grained vegetable carbon that is obtained from the pyrolysis of different types of plant biomass, and, if added to the soil, it can improve soil characteristics and at the same time it can reduce carbon emissions. Biochar also appears to have an unclear role in the activation of systemic resistance responses to pathogens. Biochar has a carbon content of about 90%; its high porosity increases the retention of water and nutrients by reducing the need for water and fertilizers and increasing agricultural yield. Aphids are one of the major sources of biotic stress for the tomato (Solanum lycopersicum), a crop of significant agro-food and socio-economic importance, especially in the Mediterranean area and in southern Italy. In this study, we first evaluated, through a proteomic analysis, the differential protein expression of tomato leaves infected by aphid and grown on control soils and on biochar-modified soil. The results of the proteomic analysis showed a differential expression mainly in the proteins involved in stress and defense, so we decided to deepen this aspect through a molecular analysis. A Real-time PCR of some fundamental genes involved in the Jasmonic acid pathway was made because, although it is clear that aphid infection activates the salicylic acid pathway, we have less data in literature about the resulting tissue damage involves Jasmonic Acid (JA). The regulation of jasmonic acid after phytophagous insects attack is particularly important for the plant’s ability to initiate promptly to the defense responses.
Theory matters! Efficiency measurement and water utilities

As is the case of other infrastructure sectors the availability of efficiency estimation software based on statistical inference – freely distributed via the internet and relatively easy to use – recently inflated the number of corresponding applications in the water sector. The robustness of regulatory measures based on inferences from efficiency measures nevertheless crucially depends on theoretically well-founded estimates. This is illustrated by using an empirical example of an inconsistent technical efficiency frontier for water utilities in Germany.
Constructing academic discourse : dialogic instruction in an EFL film class

In this paper, I present a practical, grounded analysis of a first-year university English film class, with the goal to chronicle both the elements of instruction and what students thought about class activities. As instructor, I have tried to adopt a dialogic approach to develop academic proficiency, grounded in the oral interaction of wholeclass discussion that aims to have students think critically and express ideas in a creative, productive manner. I draw on reflective teacher journals and anonymous student surveys, to describe how I have taught the course during my career at Keio University. I begin by noting the films selected, and discuss such features as how the quizzes were enacted to ensure that everyone did the homework. I also note how discussion was organized to improve both language skills and discursive argumentation, and how written essays were assigned to extend classroom discussion. I conclude by noting that shared talk within a supportive environment stimulated critical thinking, suggesting new identifications with English and carrying broad implications for EFL pedagogy.
Microfluidic platform for bilayer experimentation: from a research tool towards drug screening

Ion channels, which are located in the membranes of cells, are responsible for a number of physiological processes. Mutations in genes encoding ion channels resulting in alteration of their function can be associated with diseases, which makes them attractive targets for drug screening. In this thesis, a microfluidic device for bilayer experimentation has been developed, and its possible utilization for drug screening on ion channels discussed. First, various technical aspects have been considered such as the design and fabrication of the microfluidic platform, a reliable protocol for bilayer formation has been established, and the coupling of the platform to various detection techniques including high resolution confocal imaging and electrophysiological measurements has been achieved. The potential of the platform has been demonstrated through electrophysiological measurements of ion channel models (α-hemolysin and gramicidin) down to the single molecule level, and by proof-of-principle experiments to sense changes in the pore formation of the peptide gramicidin after its exposure to external soluble factors (ethanol & acetylsalicylic acid). The combination of optical and high resolution confocal imaging with electrophysiological measurements has particularly been applied to study the impact of cholesterol on the bilayer properties (thickness and fluidity) and their effect on the ion channel function. Finally, the multiplexing of the device has been demonstrated. While most of these developments have been driven by the motivation to develop a drug-screening platform, a technology assessment exercise has been conducted to identify alternative applications and to foresee possible hurdles that could be encountered during technology transfer and product development.