Miracle-dz/UniFAR-FTUs huggingface dataset:
The Tomato SlVIPP1 Gene Is Required for Plant Survival Through the Proper Development of Chloroplast Thylakoid Membrane

Since membranes play essential roles in all living beings, all cells have developed mechanisms for efficient and fast repair of membrane damage. In Escherichia coli, the Phage shock stress A (PspA) protein is involved in the maintenance of the integrity of its inner membrane in response to the damage produced by exposure to stress conditions. A role in thylakoid membrane maintenance and reorganization has been proposed for Vesicle Inducing Protein in Plastid 1 (VIPP1), the putative PspA ortholog in Arabidopsis thaliana. While some membranes of plant cells have been extensively studied, the biosynthesis and maintenance of chloroplast thylakoid membrane remains poorly known. Here, we report the cloning and functional characterization of the tomato (Solanum lycopersicum L.) ortholog of Escherichia coli PspA and Arabidopsis thaliana VIPP1, which we dubbed SlVIPP1. Our genetic and molecular characterization of slvipp1, an insertional mutant, allowed us to conclude that the tomato SlVIPP1 gene is needed for development, as Arabidopsis VIPP1, but not Escherichia coli PspA. Homozygous slvipp1 tomato plants are albino and exhibit early lethality and highly aberrant chloroplast development with almost complete absence of thylakoids. The phenotype of tomato RNAi lines and that of additional slvipp1 alleles generated by CRISPR/Cas9 gene editing technology confirmed that the morphological and histological aberrations shown by slvipp1 homozygotes are caused by VIPP1 lack of function. We also found that tomato SlVIPP1 overexpression does not cause any visible effect on plant morphology and viability. Our work with slvipp1 plants evidences that SlVIPP1 is an essential gene required for tomato survival, since its function is crucial for the proper formation and/or maintenance of thylakoid membranes.
Optimization of pricklyash seed protein hydrolyzing by Alcalase proteases to prepare antioxidative peptides

In this study,in order to optimize the enzymolysis conditions of pricklyash seeds antioxidative peptide,the degree of hydrolysis( DH) and DPPH radical scavenging capacity of pricklyash seed protein antioxidative peptide as an indicator,the enzymolysis conditions,including enzymes type,enzyme concentration,substrate concentration,reaction temperature,time and so on were investigated.The results indicated that better effective was observed with Alcalase proteases hydrolysis pricklyash seed protein to prepare antioxidative peptides.Through the response surface methodology,enzymatic antioxidative peptide conditions of pricklyask seeds protein were optimized.The optimum conditions were substrate concentration of 5%,the temperature 55℃,pH 8.5,4% of the amount of enzyme.The optimum method could be applied in the producing process of pricklyash seeds antioxidative peptide.Based on this condition,hydrolysate of pricklyash seeds protein performed higher antioxidative activity with 61.00% DPPH scavenging capacity,12.00% degree of hydrolysis.
Rare Case of Sarcomatoid Renal Cell Carcinoma Metastasis to the Duodenum: Report of a Case and Review of the Literature

Metastatic Sarcomatoid Renal Cell Carcinoma has been shown to be a diagnosis that holds a median survival time of under a year. Top metastases locations have been reported to be the lung, retroperitoneal lymph nodes, and axial skeleton. Tutor markers vary widely, making the histological understanding of this disease particularly important. Whether higher percentage of sarcomatoid histopathology corresponds with a worse prognosis or not is also highly debated in the literature. Here we present a case of Sarcomatoid Renal Cell Carcinoma that has metastasized to the Duodenum, which to the best of our knowledge, is the first reported case. We hope to raise awareness of the potential duodenum metastasis and corresponding tumor markers of Sarcomatoid Renal Cell Carcinoma to aid in the treatment of future patients.
Re-establishment, synopsis and new combinations in the genus Bionia Mart. ex Benth. (Leguminosae: Papilionoideae)

Bionia Mart.ex Benth. is a small genus of tribe Phaseoleae subtribe Diocleinae (Leguminosae). It was considered as a synonym of Camptosema W.J.Hook. & Arn. for the last 160 years. The genus is re-established based on previous phylogenetic analysis of the subtribe Diocleinae which showed that Bionia is unrelated to Camptosema . Bionia is distinct from Camptosema by macromorphological and palynological characters and is restricted to eastern and central Brazil. New combinations are presented here, resulting in a circumscription of Bionia including five species.
Movements of Western Chorus Frogs Pseudacris triseriata triseriata Tagged with Co 60

The movements of Pseudacris triseriata triseriata tagged with Co60 are reported. A total of 324 recaptures involving 62 specimens was made from 25 March to 4 August 1970. Dispersal from the breeding pools was gradual. Most specimens remained within 100 m of the breeding pools where they were tagged but 15 moved farther and two moved away over 200 m. The mean daily rate of movement was 3.5 m/day but the greatest rate of movement observed was 42 mn/day. The frogs tended to remain hidden among leaf litter during the daylight hours and their daily period of activity appeared to occur between dusk and dawn.
Abstract Behavior Representations for Service Integration

Behavior Representations for Service Integration Ion Constantinescu, Steve Willmott, Boi Faltings Artificial Intelligence Laboratory, Swiss Federal Institute of Technology, IN (Ecublens), CH-1015 Lausanne, Switzerland. {ion.constantinescu,steven.willmott,boi.faltings}@epfl.ch Abstract: Current web infrastructure is oriented towards humanmachine interactions. Developments for next generation systems (such as Web Services and ebXML) however aim to allow for automated interactions between arbitrary systems / services. This is achieved by supplying high-level descriptions of service capabilities and enabling information systems to dynamically discover and access each other. Current web infrastructure is oriented towards humanmachine interactions. Developments for next generation systems (such as Web Services and ebXML) however aim to allow for automated interactions between arbitrary systems / services. This is achieved by supplying high-level descriptions of service capabilities and enabling information systems to dynamically discover and access each other. This paper takes into account 5 well-known behavioral description languages (PDDL, DAML-S, WSFL, ebXML and ConGolog), tries to propose an abstract behavior representation and gives a mapping between it and the existing formalisms.
Properties of rhodanese from the liver of tilapia, Oreochromis niloticus, in Asejire Lake, Nigeria

The study investigates the purification and characterisation of rhodanese from the liver of the tilapia fish (Oreochromis niloticus) collected from Asejire Lake in Nigeria. This was with a view to understanding the biochemical basis of the survival of the fish in cyanide polluted water. Rhodanese was isolated and purified from liver tissue homogenate of tilapia using CM-Sephadex ion exchange chromatography and Sephadex G-75 gel filtration. The specific activity of the enzyme was 56.86 U/mg. The Km values for KCN and Na2S2O3 as substrates were 0.1240 ± 0.0021 mM and 0.0516 ± 0.0097 mM, respectively. The apparent molecular weight was estimated by gel filtration on a Sephacyl S-400 column to be 35,460 Da. The optimal activity was found at pH 6.5 and the temperature optimum was 40°C. The rhodanese enzyme showed that the activity of the enzyme was not affected by MgCl2, KCl, NH4Cl, MnCl2 and CaCl2 while AlCl3, inhibited the enzyme. Key words: Cyanide, detoxification, tilapia, liver, rhodanese.
Amino acid requirements of growing pigs. 7. The response of pigs from 25 to 55 kg live weight to dietary ideal protein

One hundred and twenty pigs were used in a 3 × 8 factorial design. The factors were sex, 40 trios of littermate boars, castrated males and gilts, and dietary lysine, eight concentrations from 7×5 to 14×5 g/kg diet with 1 g/kg diet intervals of lysine with corresponding crude protein (CP) concentrations from 114 to 226 g/kg diet. Increases in dietary lysine were associated with concomitant increases in the other essential amino acids so as to provide an ideal protein. All diets had similar digestible energy values (13·65 MJ/kg). Pigs were fed once daily on a restricted scale from 25 to 55 kg live weight. For growth and carcass characteristics there was a range of linear response followed by a plateau. The inflexion points of dietary lysine for growth characteristics were at 11·1, 10·2 and 10·9 g/kg diet (171, 157 and 168 g CP per kg diet) together with daily intakes of 18·0, 16·7 and 17·6 g (daily intake of 277, 257 and 271 g CP) for boars, castrated males and gilts respectively. The equivalent points for carcass characteristics were 10·4, 9·7 and 10·1 g/kg (160, 149 and 156 g CP per kg diet) with daily intakes of 16·9, 15·9 and 16·4 g lysine (daily intakes of 260, 245 and 252 g CP). Blood urea data gave good support for these observations.
Assessing Forest/Non-Forest Separability Using Sentinel-1 C-Band Synthetic Aperture Radar

Synthetic Aperture Radar has a unique potential for continuous forest mapping as it is not affected by cloud cover. While longer wavelengths, such as L-band, are commonly used for forest applications, in this paper we assess the aptitude of C-band Sentinel-1 data for this purpose, for which there is much interest due to its high temporal resolution (five days) and “free, full, and open” data policy. We tested its ability to distinguish forest from non-forest in six study sites, located in Alaska, Colombia, Finland, Florida, Indonesia, and the UK. Using the time series for a full year significantly increases the classification accuracy compared to a single scene (a mean of 85 % compared to 77 % across the study sites for the best classifier). Our results show that we can further improve the mean accuracy to 87 % when only considering the annual mean and standard deviation of co-polarized (VV) and cross-polarized (VH) backscatter. In this case, separation accuracies of up to 93 % (in Finland) are possible, though in the worst case (Alaska), the highest possible accuracy using these variables was 80 % . The best overall performance was observed when using a Support Vector Machine classifier, outperforming random forest, k-Nearest-Neighbors, and Quadratic Discriminant Analysis. We further show that the small information content we found in the phase data is an artifact of terrain slope orientation and has a negligible impact on classifier performance. We conclude that for the purposes of forest mapping the smaller file size and easier to process GRD products are sufficient, unless the SLC products are used to compute the temporal coherence which was not tested in this study.
Abstract 4309: Establishment of the chick chorioallantoic membrane (CAM) as an ex vivo model system to study mechanisms underlying epithelial ovarian tumour growth and metastasis

Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL Epithelial ovarian cancer (EOC) accounts for approximately 90% of all human ovarian malignancies. Unfortunately, 75% of EOC cases are not diagnosed until advanced stages when the tumour has metastasized, after which standard therapy is less effective. Therefore, it remains vital to directly study the molecular and cellular mechanisms contributing to EOC metastasis in an experimentally-tractable system. The chick chorioallantoic membrane (CAM) is a highly-vascularized tissue responsible for gas and nutrient exchange in the avian embryo, and has been used extensively to study angiogenesis and tumour formation by highly-aggressive cancer cell lines. Herein, we report that ascites-derived primary human EOC cells and established EOC cell lines readily form well-vascularized tumours on the chick CAM. To investigate this property further, we have generated GFP-expressing clones from the mouse ovarian tumour cell lines MOSE-RM (oncogenic Ras & Myc overexpression, SV40 TAg), MASC-2 (SV40 TAg), and 4306 (oncogenic Kras & Pten-null) which will facilitate visualization of tumour growth on the CAM as well as complementary studies in syngeneic mouse models for future studies. Xenografts of MOSE-RM and 4306 cell lines onto the surface of the chick CAM establish tumours that are significantly larger in mass and size compared with MASC-2 cells. Importantly, EOC tumours grown on the chick CAM are histologically identical to those arising within the peritoneal cavity. MOSE-RM and 4306 cell lines promote angiogenesis using HUVEC cells and the chick CAM, whereas MASC-2 cells lack this ability. Overall, these results demonstrate that EOC tumour growth on the chick CAM can be exploited as a highly amenable model system for studying late-stage EOC metastasis. Our findings also indicate that neo-vascularization is an obligatory process for establishment of secondary tumours; thus, we are currently investigating signaling pathways that impact primary human EOC tumour growth and angiogenesis using the chick CAM. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4309. doi:10.1158/1538-7445.AM2011-4309
Within-population variation in the relation between node number and flowering time in Rhinanthus angustifolius (Orobanchaceae)

Background and aims – The annual hemiparasitic plant genus Rhinanthus displays large variation in the date of onset of flowering, and ecotypes have been described from populations with different flowering times. Much less is known, however, about the variation in flowering time within populations of an ecotype, which is important for the adaptive capacity of a population. The number of nodes produced before the first flower is an important trait linked to flowering time differences among populations, and this trait and its relation with flowering date were investigated. Methods – Seeds from a natural, early-flowering population of Rhinanthus angustifolius, mown in early July, were used to establish a new field population in 2003, mown after summer, and to cultivate plants in the greenhouse in 2001 and 2004. The onset of flowering, node number and plant size were recorded in the field population in 2005 and in 2008. In the greenhouse, germination date, node number and flowering date were recorded. Key results – Flowering time was strongly correlated with node number in all years: the production of more nodes before the first flower was associated with a delay in flowering. There was always considerable variation around the median flowering date among plants with the same number of nodes, even in the greenhouse. Removing variation in the timing of germination in the greenhouse experiment did not reduce variation in flowering date. Part of the remaining variation was due to variation in plant size: larger plants flowered earlier. After five years, the relaxed selection on flowering time by mowing later had increased node number and delayed flowering in the new field population. Conclusions – Both genetically determined (node number) and phenotypically plastic (plant size) traits contribute to variation in flowering time within populations, and even under strong selection against late flowering, wild populations may harbour enough variation to react to a decrease in this selection pressure by later mowing.
Déterminismes des assemblages de gastéropodes aquatiques en zones alluviales: (Rive sud du lac de Neuchâtel-CH et Basse plaine de l'Ain-F)

L'objectif de ce travail de these est, sur la base de l'etude comparative de la composition et de la repartition des peuplements dans differents systemes et a differentes echelles spatiales (secteurs, ecosystemes, habitats), de voir dans quelle mesure les gasteropodes aquatiques peuvent etre les revelateurs signifiants des facteurs de regulation de la diversite biologique generale des zones humides dans les systemes alluviaux. Pour repondre a cet objectif, nous nous sommes attaches a: determiner la part respective des influences dues a des facteurs locaux (structure du micro-habitat) par rapport a des facteurs plus globaux tels que la connectivite ; decrire et comparer les reponses de diverses mesures de la diversite des gasteropodes (richesse specifique, composition des assemblages et occurrence d'especes individuelles) ; developper une approche comparative entre deux systemes alluviaux presentant des fonctionnements hydrologiques differents: un milieu alluvial fluvial dynamique et un milieu alluvial peri-lacustre, moins fluctuant.
Studies of Grain Boundaries in Materials Subjected to Diffusional Creep

max. 2000 characters) Grain boundaries in crystalline Cu(2%Ni) creep specimens have been studied by use of scanning and transmission electron microscopy in order to establish the mechanism of deformation. Creep rate measurements and dependencies were found to fit reasonably well with the model for diffusional creep (NabarroHerring) and this impression was strengthened considerably by the microstructural finds. Manual and automated EBSD surface scans have been performed on a specimen supplied with an inert reference grid in order to correlate individual grain boundary structure (in terms of deviation from CSL) with the activity displayed during diffusional creep testing. It was found that boundaries with low deviation from perfect Σ did not contribute macroscopically to the creep strain. A resist deposition procedure was examined to improve the reference surface grid so as to allow determination of the grain boundary plane by use of simple stereomicroscopy directly on the surface. The etched pattern deteriorated heavily during creep testing, supposedly because of dislocation creep, due to excessive creep stress. Grain boundaries have been studied and characterised by TEM providing an insight into the diversity of twin boundary structures. Ledges on low deviation Σ3 and Σ9 were observed repeatedly and the presence of these is proposed to relate to the inactivity of such boundaries. A handful of examples showed ledged boundaries emitting dislocations into the lattice, presumably because of stress accumulation due to restrictions on GBD movement from the ledge structure.
Performance of Commercial Laying Hens Fed Various Supplemental Amino Acids in a Corn-Soybean Meal Diet

Abstract Two experiments using Hy-Line® W36 pullets, 26 wk of age, helped determine their lysine (Lys) requirement and the next limiting amino acid in a corn-soybean diet. Corn-soybean meal diets ranging from 16.68 to 13.79% protein were fed in Experiment 1. All diets contained supplemental methionine (Met). The diets were fed with and without supplemental Lys. When unsupplemented diets were fed, Lys intake ranged from 754 to 502 mg/hen/day. The daily Lys requirement was 685.7, 677.4, and 755 mg/hen/day for egg mass (EM), egg production (EP), and egg weight (EW), respectively. When the diet provided less than 560 mg/hen/day of Lys, supplemental Lys did not restore EP. This finding indicated that another amino acid was limiting. In Experiment 2, various combinations of supplemental tryptophan (Trp), arginine (Arg), threonine (Thr), isoleucine (Ile), valine (Val), and glutamic acid (Glu) were fed. Hens receiving the negative control diet during 31-34 wk had daily intakes of 540, 505, 668, 395, 115, 426, 480, and 1968 mg/hen/day total sulfur amino acids (TSAA), Lys, Arg, Thr, Trp, Val, Ile, and Glu, respectively. Supplementing the diet with Met and Lys significantly increased EM. Further improvement in EM resulted from the combination of Thr, Arg, and Trp supplementation. The addition of Val to the diet with Met, Lys, Thr, Arg, and Trp significantly increased EM. However, the addition of Ile was without benefit in the presence or absence of Val. Egg mass was not significantly increased with a diet that contained all amino acids, with Val becoming next limiting after these. In a corn soybean meal diet Met is the first limiting EAA, followed by Lys. Trp and Arg are the next limiting, followed by Val.
Rapid Extraction of Plasmid DNA from Escherichia coli Using Magnetic Bead Separation Method

In this paper,carboxyl-group functionalized single-dispersed magnetic nanoparticles were used as solid phase to extract plasmid DNA from crude Escherichia coli lysate by using PEG/NaCl as binding buffer.The results indicated that when the PEG/NaCl binding buffer was 7.5%/1.25 mol/L,at room temperature,8.3 μg of plasmid DNA which A260/A280 ratio stabilized at around 1.8 can be harvested from 1.5 mL overnight cultured E.coli bacterium by using 0.9 mg magnetic nanoparticles.The time needed was only 20 min.Compared with conventional methods,the magnetic bead separation method is efficient,convenient,economic and security,not only suitable for small scale preparation of pDNA at laboratory,but also can be adopted to construct automated pDNA analysis and identification platforms.
Risk Factors of Mortality among Male Patients with Cardiovascular Disease in Malaysia Using Bayesian Analysis

Background: Identifying risk factors associated with mortality is important in providing better prognosis to patients. Consistent with that, Bayesian approach offers a great advantage where it rests on the assumption that all model parameters are random quantities and hence can incorporate prior knowledge. Therefore, we aimed to develop a reliable model to identify risk factors associated with mortality among ST-Elevation Myocardial Infarction (STEMI) male patients using Bayesian approach. Methods: A total of 7180 STEMI male patients from the National Cardiovascular Disease Database-Acute Coronary Syndrome (NCVD-ACS) registry for the years 2006–2013 were enrolled. In the development of univariate and multivariate logistic regression model for the STEMI patients, Bayesian Markov Chain Monte Carlo (MCMC) simulation approach was applied. The performance of the model was assessed through convergence diagnostics, overall model fit, model calibration and discrimination. Results: A set of six risk factors for cardiovascular death among STEMI male patients were identified from the Bayesian multivariate logistic model namely age, diabetes mellitus, family history of CVD, Killip class, chronic lung disease and renal disease respectively. Overall model fit, model calibration and discrimination were considered good for the proposed model. Conclusion: Bayesian risk prediction model for CVD male patients identified six risk factors associated with mortality. Among the highest risks were Killip class (OR=18.0), renal disease (2.46) and age group (OR=2.43) respectively.
Ontological Representation of Knowledge for Developing Information Services in Food Science and Technology

Knowledge explosion in various fields during recent years has resulted in the creation of vast amounts of on-line scientific literature. Food Science &Technology ( FST ) is also an important subject domain where rapid developments are taking place due to diverse research and development activities. As a result, information storage and retrieval has become very complex and current information retrieval systems (IRs) are being challenged in terms of both adequate precision and response time. To overcome these limitations as well as to provide natural-language based effective retrieval, a suitable knowledge engineering framework needs to be applied to represent, share and discover information. Semantic web technologies provide mechanisms for creating knowledge bases, ontologies and rules for handling data that promise to improve the quality of information retrieval. Ontologies are the backbone of such knowledge systems. This paper presents a framework for semantic representation of a large repository of content in the domain of FST .