Miracle-dz/UniFAR-FTUs huggingface dataset:
Bothrops moojeni snake venom induces an inflammatory response in preadipocytes: Insights into a new aspect of envenomation

Bothrops envenomation is a public health problem in Brazil. Despite the advances in the knowledge of the pathogenesis of systemic and local effects induced by Bothrops venom, the target tissues to this venom are not completely characterised. As preadipocytes are important cells of the adipose tissue and synthesize inflammatory mediators, we investigated the ability of B. moojeni snake venom (Bmv) to stimulate an inflammatory response in 3T3-L1 preadipocytes in vitro, focusing on (1) the release of PGE2, IL-6, TNF-α, MCP-1, KC, leptin and adiponectin; (2) the mechanisms involved in PGE2 release and (3) differentiation of these cells. Cytotoxicity of Bmv was determined by MTT assay. The concentrations of PGE2, cytokines and adipokines were quantified by EIA. Participation of the COX-1 and COX-2 enzymes, NF-κB and PGE2 receptors (EP1-4) was assessed using a pharmacological approach, and protein expression of the COX enzymes and P-NF-κB was analysed by western blotting. Preadipocyte differentiation was quantified by Oil Red O staining. Bmv (1 μg/mL) induced release of PGE2, IL-6 and KC and increased expression of COX-2 in preadipocytes. Basal levels of TNF-α, MCP-1, leptin and adiponectin were not modified. Treatment of cells with SC560 (COX-1 inhibitor) and NS398 (COX-2 inhibitor) inhibited Bmv-induced PGE2 release. Bmv induced phosphorylation of NF-κB, and treatment of the cells with TPCK and SN50, which inhibit distinct NF-κB domains, significantly reduced Bmv-induced PGE2 release, as did the treatment with an antagonist of PGE2 receptor EP1, unlike treatment with antagonists of EP2, EP3 or EP4. Bmv also induced lipid accumulation in differentiating cells. These results demonstrate that Bmv can activate an inflammatory response in preadipocytes by inducing the release of inflammatory mediators; that PGE2 production is mediated by the COX-1, COX-2 and NF-κB pathways; and that engagement of EP1 potentiates PGE2 synthesis via a positive feedback mechanism. Our findings highlight the role of the adipose tissue as another target for Bmv and suggest that it contributes to Bothrops envenomation by producing inflammatory mediators.
Prevalence of psychological distress among cancer patients in Southeast Asian countries: A systematic review

Abstract Psychological distress (including depression and anxiety) is common in the first years of cancer diagnosis but can differ by country and region. The aim of the present paper was to review the prevalence of psychological distress among cancer patients in the Southeast Asia (SEA) region. A systematic literature search was carried out using several databases (i.e., PubMed, PsychARTICLES, Embase, CINAHI, Web of Sciences, Plus, Scopus, and AHMED). Papers originally published in English language were taken into consideration if they (i) were published from 2010 to 2021 and (ii) reported the prevalence of psychological distress among patients with different types of cancer. A total of 23 studies met the inclusion criteria. The most frequently employed psychometric instrument for anxiety and depression screening was the Hospital Anxiety and Depression Scale (HADS). The prevalence of anxiety (ranging from 7% to 88%) was wider than that of depression (ranging from 3% to 65.5%) among patients with different types of cancer and living in various countries in the SEA region. The overall prevalence rate of psychological distress among cancer patients from the SEA region was not fundamentally very different from that of general populations. These findings provide useful information for health professionals and cancer patients to understand the negative role of psychological distress in quality of life and health. The research findings demonstrate the importance of counselling for psychological distress among cancer patients as means of effectively resolving their psychological problems and ultimately improving the quality of oncology medical care. Clinical recommendations for cancer management should incorporate the early identification of (and therapy for) psychological distress, as well as their monitoring during treatment.
Automated slice‐specific z‐shimming for functional magnetic resonance imaging of the human spinal cord

Functional magnetic resonance imaging (fMRI) of the human spinal cord faces many challenges, such as signal loss due to local magnetic field inhomogeneities. This issue can be addressed with slice‐specific z‐shimming, which compensates for the dephasing effect of the inhomogeneities using a slice‐specific gradient pulse. Here, we aim to address outstanding issues regarding this technique by evaluating its effects on several aspects that are directly relevant for spinal fMRI and by developing two automated procedures in order to improve upon the time‐consuming and subjective nature of manual selection of z‐shims: one procedure finds the z‐shim that maximizes signal intensity in each slice of an EPI reference‐scan and the other finds the through‐slice field inhomogeneity for each EPI‐slice in field map data and calculates the required compensation gradient moment. We demonstrate that the beneficial effects of z‐shimming are apparent across different echo times, hold true for both the dorsal and ventral horn, and are also apparent in the temporal signal‐to‐noise ratio (tSNR) of EPI time‐series data. Both of our automated approaches were faster than the manual approach, lead to significant improvements in gray matter tSNR compared to no z‐shimming and resulted in beneficial effects that were stable across time. While the field‐map‐based approach performed slightly worse than the manual approach, the EPI‐based approach performed as well as the manual one and was furthermore validated on an external corticospinal data‐set (N > 100). Together, automated z‐shimming may improve the data quality of future spinal fMRI studies and lead to increased reproducibility in longitudinal studies.
A Multilayer and Event-Triggered Voltage and Frequency Management Technique for Microgrid’s Central Controller Considering Operational and Sustainability Aspects

This paper proposes a management technique which will retain the voltage and frequency of standalone microgrids (MGs) within a predefined safe zone at least cost using a multilayer scheme. The proposed technique considers the sustainability and dynamic supply adequacy aspects of the MG whilst considering the operational costs which accrue during the continuous variations of its demand and renewable generation. This technique takes action immediately following the occurrence of events that violate the MGs voltage or frequency. It then adjusts the optimal generation levels for the dispatchable sources and determines the best configuration of the MG’s network. It also proceeds to optimize the internal support by controlling the charge or discharge power of the existing battery storages as well as curtailing the renewable sources and adjusting the loads. The proposed technique is a short-horizon operation-stage algorithm which uses a metaheuristic optimization at the MG’s central controller to analyze the status and costs of the MG components to determine the appropriate actions to implement. The performance of the developed technique is validated through extensive numerical analyzes in MATLAB.
Preferred music lowers anxiety levels and pain perception while promoting patient satisfaction in women undergoing ultrasound-guided breast biopsy: randomized controlled study

Background Image-guided breast biopsies are well accepted, yet stressful procedures for patients in radiology clinics. Purpose To investigate the effect of music on anxiety, pain, and patient satisfaction related to a breast biopsy session. Material and Methods After approval of institutional review board and giving informed consent, 93 women scheduled for ultrasound-guided breast biopsy or stereotactic wire localization were prospectively enrolled in this randomized controlled study. Patients were referred into two groups either listening to music of their own choice from three options of genre list during the procedure or not. Immediately before and after the biopsy session, participants were asked to complete questionnaires for measuring anxiety (State-Trait Anxiety Inventory, Beck Anxiety Inventory), pain, and patient satisfaction (visual analog scale). Sociodemographic data were described, and the duration was noted at the end. Statistical analysis was made using paired samples t-test, chi-square test, independent samples t-test, and Mann–Whitney U test. Results Except for the duration, demographic characteristics were statistically similar between the two groups. Trait and preprocedural state anxiety scores did not have significant difference between the groups. Both groups showed lowered state anxiety values after the biopsy (P < 0.05) and there was statistically significant reduction in state anxiety levels of music group (10.35 ± 7.5 music vs. 7 ± 7.98 control; P = 0.024). Pain perception was fewer (P < 0.05) and patient satisfaction was greater (P < 0.05) through the implementation of music. Conclusion Music intervention reduces anxiety and pain and thereby increases patient satisfaction during ultrasound-guided breast biopsies.
Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes

he mechanisms that govern the assembly of nuclear pore complexes (NPCs) remain largely unknown. Here, we have established a role for karyopherins in this process. We show that the yeast karyopherin Kap121p functions in the targeting and assembly of the nucleoporin Nup53p into NPCs by recognizing a nuclear localization signal (NLS) in Nup53p. This karyopherin-mediated function can also be performed by the Kap95p–Kap60p complex if the Kap121p-binding domain of Nup53p is replaced by a classical NLS, suggesting a more general role for karyopherins in NPC assembly. At the NPC, neighboring nucleoporins bind to two regions in Nup53p. One nucleoporin, T Nup170p, associates with a region of Nup53p that overlaps with the Kap121p binding site and we show that they compete for binding to Nup53p. We propose that once targeted to the NPC, dissociation of the Kap121p–Nup53p complex is driven by the interaction of Nup53p with Nup170p. At the NPC, Nup53p exists in two separate complexes, one of which is capable of interacting with Kap121p and another that is bound to Nup170p. We propose that ﬂuctuations between these two states drive the binding and release of Kap121p from Nup53p, thus facilitating Kap121p’s movement through the NPC.
Healthy Lifestyle through Young Adulthood and Presence of Low Cardiovascular Disease Risk Profile in Middle Age

: Background - A low cardiovascular disease (CVD) risk profile (untreated cholesterol < 200 mg/dl, untreated blood pressure < 120/<80 mmHg, never smoking, and no history of diabetes and myocardial infarction) in middle age is associated with markedly better health outcomes in older age, but few middle aged adults have this low risk profile. We examined whether adopting a healthy lifestyle throughout young adulthood is associated with presence of the low CVD risk profile in middle age. Methods and Results - The CARDIA study sample consisted of 3,154 black and white participants aged 18 to 30 years at Year 0 (Y0, 1985-86) who attended the Year 0, 7 and 20 (Y0, Y7 and Y20) examinations. Healthy lifestyle factors (HLFs) defined at Y0, Y7 and Y20 included: 1) Average BMI < 25 kg/m 2 ; 2) No or moderate alcohol intake; 3) higher healthy diet score; 4) higher physical activity score; and 5) Never smoking. Mean age (25 years) and percentage of women (56%) were comparable across groups defined by number of HLFs. The age-, sex- and race-adjusted prevalences of low CVD risk profile at Y20 were 3.0%, 14.6%, 29.5%, 39.2% and 60.7% for people with 0 or 1, 2, 3, 4, and 5 HLFs, respectively (p-trend <0.0001). Similar graded relationships were observed for each sex-race group (all p-trend<0.0001). Conclusions - Maintaining a healthy lifestyle throughout young adulthood is strongly associated with low CVD risk profile in middle age. Public health and individual efforts are needed to improve adoption and maintenance of healthy lifestyles in young adults.
Intercropping and their application Grain yield stability of individual cereal-legume mixtures grown across Europe

Mixed cultivation of two or more crop species on the same piece of land at a given time may enhance crop yield stability. We assessed yield stability by first calculating the expected coefficients of variance (CV%) for cereal-legume mixtures grown across contrasting environments based on the corresponding yield variation of the mixture components grown separately in pure cultures; and then dividing the expected CV% by the observed CV% for each mixture. The ratio between the expected and observed CV% is the stability index, with values >1 indicating greater stability of the mixed crops and values <1 indicating greater stability of the crops grown separately. Stability indices for eight out of 11 barley/pea and wheat/faba bean mixtures grown across Europe were between 0.7 and 1.8 (mean 1.0), whilst two barley/pea mixtures had indices >3.7. The results are relevant in the discussion of crop mixtures as means to increase yield stability.
Advancements in the Design and Development of CubeSat Attitude Determination and Control Testing at the Virginia Tech Space Systems Simulation Laboratory

Among the various challenges involved in the development of CubeSats lies the attitude determination and control of the satellite. The importance of a properly functioning attitude determination and control system (ADCS) on any satellite is vital to the satisfaction of its mission objectives. Due to this importance, three-axis attitude control simulators are commonly used to test and validate spacecraft attitude control systems before flight. However, these systems are generally too large to successfully test the attitude control systems on-board CubeSat-class satellites. Due to their low cost and rapid development time, CubeSats have become an increasingly popular platform used in the study of space science and engineering research. As an increasing number of universities and industries take part in this new approach to small-satellite development, the demand to properly test, verify, and validate their attitude control systems will continue to increase. An approach to CubeSat attitude determination and control simulation is in development at the Virginia Tech Space Systems Simulation Laboratory. The final test setup will consist of an air bearing platform placed inside a square Helmholtz cage. The Helmholtz cage will provide an adjustable magnetic field to simulate that of a low earth orbit (LEO), and the spherical air bearing will simulate the frictionless environment of space. In conjunction, the two simulators will provide an inexpensive and adjustable system for testing any current, and future, CubeSat ADCS prior to flight. Using commercial off the shelf (COTS) components, the Virginia Tech CubeSat Attitude Control Simulator (CSACS), which is a low cost, lightweight air bearing testing platform, will be coupled with a 1.5-m-long square Helmholtz cage design in order to provide a simulated LEO environment for CubeSat ADCS validation. Advancements in the Design and Development of CubeSat Attitude Determination and Control Testing at the Virginia Tech Space Systems Simulation Laboratory
“Falling in love with me means that she can never go back”: a case study exploring the cumulative costs of multiple migrations

Abstract This case study centers on the narrative accounts of a lesbian couple in a binational relationship (i.e., two women from different countries) who left the United States (U.S.) at a time when immigration laws did not recognize their union. Given that they could not legally stay in the U.S. together, they immigrated to the Netherlands and subsequently to Canada. When DOMA was overturned and the couple was eligible to apply for immigration to the U.S., they opted to remain in Canada, in part due to the strain of multiple migrations. This concept has been described as the complex process that accompanies more than one immigration experience and the cumulative effect that this can have on couples and families over time, particularly when multiple resettlements have taken place. A descriptive case study methodology was employed to investigate the perceptions of multiple migrations of this lesbian binational couple, focusing mainly on their experiences in Canada. Specifically, individual interviews were conducted with each woman to explore their lived experiences of multiple migration and the impact this has had on various aspects of their lives, including those occurring at the intersection of privilege and oppression. A description of each woman is presented, followed by the significant themes that emerged from this study, including immigration difficulties and the centrality of relationship. Implications for psychological training, practice, research, policy were presented.
Altered Immunoglobulin Repertoire and Decreased IgA Somatic Hypermutation in the Gut during Chronic HIV-1 Infection

The gut is a major site of early HIV-1 replication and pathogenesis. Extensive CD4+ T cell depletion in this compartment results in a compromised epithelial barrier that facilitates the translocation of microbes into the underlying lamina propria and systemic circulation, resulting in chronic immune activation. ABSTRACT Humoral immune perturbations contribute to pathogenic outcomes in persons with HIV-1 infection (PWH). Gut barrier dysfunction in PWH is associated with microbial translocation and alterations in microbial communities (dysbiosis), and IgA, the most abundant immunoglobulin (Ig) isotype in the gut, is involved in gut homeostasis by interacting with the microbiome. We determined the impact of HIV-1 infection on the antibody repertoire in the gastrointestinal tract by comparing Ig gene utilization and somatic hypermutation (SHM) in colon biopsies from PWH (n = 19) versus age and sex-matched controls (n = 13). We correlated these Ig parameters with clinical, immunological, microbiome and virological data. Gene signatures of enhanced B cell activation were accompanied by skewed frequencies of multiple Ig Variable genes in PWH. PWH showed decreased frequencies of SHM in IgA and possibly IgG, with a substantial loss of highly mutated IgA sequences. The decline in IgA SHM in PWH correlated with gut CD4+ T cell loss and inversely correlated with mucosal inflammation and microbial translocation. Diminished gut IgA SHM in PWH was driven by transversion mutations at A or T deoxynucleotides, suggesting a defect not at the AID/APOBEC3 deamination step but at later stages of IgA SHM. These results expand our understanding of humoral immune perturbations in PWH that could have important implications in understanding mucosal immune defects in individuals with chronic HIV-1 infection. IMPORTANCE The gut is a major site of early HIV-1 replication and pathogenesis. Extensive CD4+ T cell depletion in this compartment results in a compromised epithelial barrier that facilitates the translocation of microbes into the underlying lamina propria and systemic circulation, resulting in chronic immune activation. To date, the consequences of microbial translocation on the mucosal humoral immune response (or vice versa) remains poorly integrated into the panoply of mucosal immune defects in PWH. We utilized next-generation sequencing approaches to profile the Ab repertoire and ascertain frequencies of somatic hypermutation in colon biopsies from antiretroviral therapy-naive PWH versus controls. Our findings identify perturbations in the Ab repertoire of PWH that could contribute to development or maintenance of dysbiosis. Moreover, IgA mutations significantly decreased in PWH and this was associated with adverse clinical outcomes. These data may provide insight into the mechanisms underlying impaired Ab-dependent gut homeostasis during chronic HIV-1 infection.
Feeding ecology of Marcusenius senegalensis (Pisces: Osteoglossiformes: Mormyridae; Steindachner, 1870) from Niger River in Northern Benin

Also known as bulldog fish, Marcusenius senegalensis is the dominant Mormyrid in Niger River in Northern Benin where the species is intensively exploited for sale and subsistence. The current research investigated the trophic ecology of this electric fish in order to document resource exploitation and feeding habits. Marcusenius senegalensis individuals were sampled monthly and stomach contents of the 2019 individuals collected were analyzed. The results indicated that Marcusenius senegalensis is an invertivore consuming a wide spectrum of food resources dominated by aquatic insects (74.38%), detritus (8.98%), sand particles (8.19%), crustaceans (3.92%), mollusks (2.51%) and phytoplankton (1.13%). Diet breadths were high and varied between 2.22 and 4.98, leading to a trophic plasticity behavior of the species. Diet overlaps between size classes were greater and averaged (Ø jk )= 0.88. In Niger River, feeding grounds are now being severely degraded. Sustainable exploitation of Marcusenius senegalensis requires a community-based approach of habitat protection and conservation of this valuable Mormyrid.
Lower-Cost (cid:15) -Private Information Retrieval

: Private Information Retrieval (PIR), despite being well studied, is computationally costly and arduous to scale. We explore lower-cost relaxations of information-theoretic PIR, based on dummy queries, sparse vectors, and compositions with an anonymity system. We prove the security of each scheme using a ﬂexible differentially private deﬁnition for private queries that can capture notions of imperfect privacy. We show that basic schemes are weak, but some of them can be made arbitrarily safe by composing them with large anonymity systems.