Miracle-dz/UniFAR-FTUs huggingface dataset:
Title Exploring the Pullback Attractors of a Low-Order Quasigeostrophic Ocean Model : The Deterministic Case Permalink

A low-order quasigeostrophic double-gyre ocean model is subjected to an aperiodic forcing that mimics time dependence dominated by interdecadal variability. This model is used as a prototype of an unstable and nonlinear dynamical system with time-dependent forcing to explore basic features of climate change in the presence of natural variability. The study relies on the theoretical framework of nonautonomous dynamical systems and of their pullback attractors (PBAs), that is, of the time-dependent invariant sets attracting all trajectories initialized in the remote past. The existence of a global PBA is rigorously demonstrated for this weakly dissipative nonlinear model. Ensemble simulations are carried out and the convergence to PBAs is assessed by computing the probability density function (PDF) of localization of the trajectories. A sensitivity analysis with respect to forcing amplitude shows that the PBAs experience large modifications if the underlying autonomous system is dominated by small-amplitude limit cycles, while less dramatic changes occur in a regime characterized by large-amplitude relaxation oscillations. The dependence of the attracting sets on the choice of the ensemble of initial states is then analyzed. Two types of basins of attraction coexist for certain parameter ranges; they contain chaotic and nonchaotic trajectories, respectively. The statistics of the former does not depend on the initial states whereas the trajectories in the latter converge to small portions of the global PBA. This complex scenario requires separate PDFs for chaotic and nonchaotic trajectories. General implications for climate predictability are finally discussed.
Created Equal? Comparing Disturbing Media Outcomes Across Occupations

The present study was conducted in order to compare outcomes across distinct occupations that are exposed to disturbing media. Using four previously researched samples, I compared results across negative and positive outcomes such as STSD, burnout, social support, and growth. Samples included employees in roles within federal law enforcement and military legal professions. Results indicated that there were some significant differences between occupations on levels of STSD, burnout, and social support. In addition, results showed all samples measuring growth were scoring within the mid-range of scores, indicative of some growth potential. These results also showed that exposure type (Indirect, Combined Exposure) may not be an appropriate classification for roles within disturbing media, as results were not conclusive. Future research should continue to explore disturbing media between varying occupational roles while including measures such as growth. COMPARING DISTURBING MEDIA OUTCOMES ACROSS OCCUPATIONS 4 Table of
Crumbs2 mediates ventricular layer remodelling to form the adult spinal cord central canal

In the spinal cord, the adult central canal forms through a poorly-understood process termed dorsal collapse that involves attrition and remodelling of the pseudostratified dorsal ventricular layer. Here we show, in mouse, that dorsal ventricular layer cells adjacent to midline Nestin(+) radial glia downregulate the apical polarity proteins Crumbs2 (CRB2) and aPKC and delaminate in a step-wise manner; concomitantly, Nestin(+) radial glial end-feet ratchet down, to repeat this process. Nestin(+) radial glia secrete a factor that promotes cell delamination. This activity is mimicked by a secreted variant of CRB2 (CRB2S), which is specifically expressed by dorsal midline Nestin(+) radial glia. In cultured cells, CRB2S associated with apical membranes and decreased cell cohesion. Analysis of Crb2F/F/Nestin-Cre+/− mice further confirmed an essential role for CRB2 in dorsal collapse. We propose a model in which CRB2S promotes the progressive attrition of the ventricular layer without loss of overall integrity. This novel mechanism may operate more widely to promote orderly progenitor delamination.
Evaluation of S-incompatibility locus, genetic diversity and structure of sweet cherry (Prunus avium L.) genetic resources by molecular methods and phenotypic characteristics

ABSTRACT Sweet cherry is a vegetatively propagated, self-incompatible, perennial plant with a high level of heterozygosity and ancient breeding history. Maintenance and conservation of genetic resources are necessary for future breeding programmes, growers and fruit production stability. The knowledge about S-allele constitution of cultivars is very important for fruit growers and breeders. DNA molecular genetic methods were used for evaluation of genetic variability and S-genotyping within sweet cherry genetic resources. Totally, 13 different S-alleles in 29 S-locus combinations for 24 individual incompatibility groups within 153 sweet cherries accessions were detected. S3 (88 cultivars), S1 (60 cultivars) and S4 (54 cultivars), followed by S2 (34 cultivars) and S6 (28 cultivars) were the most frequent S-alleles. S-alleles distribution linked to ripening time was also analysed. A total of 117 microsatellite SSR and EST-SSR polymorphic markers were used for molecular-genetic analyses. The hierarchical cluster analysis divided sweet cherry cultivars into seven clusters. Genetic relationships of individual cultivars corresponded with genealogical and geobotanical characteristics and known breeding history. Genetic population structure analyses in seven clusters (K= 7) supported hierarchical clustering results and involved ripening period, fruit colour and country of origin to genetic diversity evaluation of germplasm collection.
Marker-Assisted Selection to Pyramid the Opaque-2 (O2) and β-Carotene (crtRB1) Genes in Maize

Maize is an excellent nutritional source and is consumed as a staple food in different parts of the world, including India. Developing a maize genotype with a combination of higher lysine and tryptophan, along with β-carotene, can help alleviate the problem of protein-energy malnutrition (PEM) and vitamin A deficiency (VAD). This study is aimed at improving lysine and tryptophan content by transferring opaque-2 (o2) gene from donor HKI163 to β-carotene-rich inbred lines viz., UMI1200β+ and UMI1230β+. For this purpose, F1, BC1F1, BC2F1, BC2F2, and BC2F3 plants were developed using an o2 line HKI163 and two β-carotene-rich inbred lines, UMI1200β+ and UMI1230β+, as the parents. Foreground selection using the associated marker umc1066 for the o2 gene and the marker crtRB1 3′TE for the crtRB1 gene was used to select the target genes. A total of 236 simple sequence repeat (SSR) markers distributed evenly across the maize genome were employed for the background selection. To fix the crtRB1 allele in the BC1F1 stage, individual plants homozygous at the crtRB1 locus and heterozygous at the o2 locus were selected and used for backcrossing to produce BC2F1 plants. Furthermore, the selected heterozygous BC2F1 plants from both crosses were selfed to obtain the BC2F2 plants, which were then selected for the target gene and selfed to generate the BC2F3 lines. From each cross, five improved lines with homozygous marker alleles for the crtRB1 and o2 genes with a recurrent parent genome (RPG) recovery ranging from 86.75 to 91.21% in UMI1200β+×HKI163 and 80.00 to 90.08% in UMI1230β+×HKI163 were identified. The improved lines had good agronomic performance and possessed high lysine (0.294–0.332%), tryptophan (0.073–0.081%), and β-carotene (6.12–7.38 µg/g) content. These improved lines can be used as genetic resources for maize improvement.
Complex symbiont-pathogen interactions inhibit intestinal repair

Pathogen-mediated damage to the intestinal epithelium activates compensatory growth and differentiation repair programs in progenitor cells. Accelerated progenitor growth replenishes damaged tissue and maintains barrier integrity. Despite the importance of epithelial renewal to intestinal homeostasis, we know little about the effects of pathogen-commensal interactions on progenitor growth. We found that the enteric pathogen Vibrio cholerae, blocks critical growth and differentiation pathways in Drosophila progenitors despite extensive damage to the epithelial tissue. We showed that inhibition of epithelial repair requires interactions of the Vibrio cholerae type six secretion system with a complex community of symbiotic bacteria, and that elimination of the gut microbiome is sufficient to restore homeostatic growth in infected intestines. Together, this work highlights the importance of pathogen-symbiont interactions on intestinal immune responses and outlines a previously undescribed impact of the type six secretion system on pathogenesis.
Modeling and Application of PMSG Based Variable Speed Wind Generation System

In the early development of wind energy, the majority of wind turbines were operated at constant speed. Recently, the number of variable-speed wind turbines installed in wind farms has increased and more wind turbine manufacturers are making variable-speed wind turbines. In this study maximum power control of wind turbine and permanent magnet synchronous generator connected with two back to back voltage source converters to grid are studied. Machine currents are controlled by indirect vector control method. In this method, generator side converter controls the maximum excitation (air gap flux) by machine's d-axis current and controls generator torque by machine's q-axis current. PMSG speed is controlled by Tip Speed Ratio (TSR) upon the wind speed variations to generate the maximum output power. Grid side converter regulates the DC link voltage and injective active power by d-axis current and regulates the injective reactive power by q-axis current using simple control method P-Q. Simulation results depict that the proposed method operates properly.
If we build it, will they use it? Phase I observational evaluation of ReaDySpeech, an online therapy programme for people with dysarthria after stroke

Abstract Purpose: To explore the acceptability of using ReaDySpeech, an online speech therapy programme for people with dysarthria after stroke, within usual clinical practice. This early clinical testing underpins future research evaluation of ReaDySpeech. Methods: A prospective, observational design involving interviews with speech and language therapists with experience of using ReaDySpeech. This included the usability of ReaDySpeech, therapists’ training/support needs, ease of recruitment of therapist and patient participants, ReaDySpeech technical issues and therapy content. Therapists also provided feedback from the patient participants. Results: Six therapists working in hospital and community-based settings used ReaDySpeech with five patients (12–28 weeks post-stroke, four female, mean age 71 years). Therapists found it was easy to use, training/support was sufficient and they reported positive feedback from participants. Areas to address involved patients’ access to Wi-Fi, ease of navigation, content improvements and difficulties recruiting people more than 12 weeks post-stroke as most patients had already been discharged. Conclusions: ReaDySpeech was acceptable and generally feasible to use in clinical practice. This early phase research testing has been essential to improve navigation within the therapy software and content. ReaDySpeech can now be further evaluated with a phase two feasibility trial with earlier recruitment following stroke.
Phenological, physicochemical, and productive characteristics of 'Vênus' grapevine onto rootstocks

Abstract: The objective of this work was to evaluate the phenological characteristics and yield of 'Vênus' grapevine (Vitis labrusca) grafted onto different rootstocks, as well the physicochemical traits of its grapes during the 2013, 2014, and 2015 crop seasons. The evaluated rootstocks were 'IAC 766', 'IAC 572', 'IAC 313' and 'IAC 571-6'. The length of the main phenological stages of the grapevine and its thermal demands were assessed during two growing seasons and expressed as days after pruning and degree-day sum, respectively. Grapevine yield and berry physicochemical characteristics were assessed during three crop seasons. 'Vênus' phenological cycle and thermal demands, as well as the chemical characteristics of its grapes, varied with the climate conditions during the crop seasons. Low temperatures increased the grapevine cycle, and higher rainfall indices caused a decrease in the soluble solids of berries, as well as an increase in their titratable acidity. The 'IAC 766' and 'IAC 571-6' rootstocks promote a greater precocity to 'Venus' grapevines, and 'IAC 572' promotes a higher grapevine yield and better physicochemical characteristics of 'Vênus' grapes.
Quality evaluation of different types of chicken consumed in Puerto Rico.

A study was done to compare the general quality, microbiology and shelf-life of fresh and frozen chicken. Two brands of frozen Grade A, one brand of frozen Grade C and one brand of fresh chicken were compared for yield, texture, general acceptability, psychrotrophic bacteria contents after one, two, and three cycles of thawing and freezing, and shelf-life once it was kept at 7.2° C (45° F) after thawing. Unfrozen fresh chicken samples were anlyzed for psychrotrophic bacteria and shelf-life as a control . Results indicated that there are no significant differences (P > 0.05) in yield, texture and general acceptability among the fresh and frozen chickens studied. The size of the psychrotrophic bacteria population is generally not affected by up to three cycles of thawing and freezing. With the exception of the frozen Grade C chickens analyzed, shelf-life was not generally affected by up to three cycles of thawing and freezing as compared with samples that were unfrozen and frozen and thawed only once.
Ataxia in Patients With Bi-Allelic NFASC Mutations and Absence of Full-Length NF186

The etiology of hereditary ataxia syndromes is heterogeneous, and the mechanisms underlying these disorders are often unknown. Here, we utilized exome sequencing in two siblings with progressive ataxia and muscular weakness and identified a novel homozygous splice mutation (c.3020-1G > A) in neurofascin (NFASC). In RNA extracted from fibroblasts, we showed that the mutation resulted in inframe skipping of exon 26, with a deprived expression of the full-length transcript that corresponds to NFASC isoform NF186. To further investigate the disease mechanisms, we reprogrammed fibroblasts from one affected sibling to induced pluripotent stem cells, directed them to neuroepithelial stem cells and finally differentiated to neurons. In early neurogenesis, differentiating cells with selective depletion of the NF186 isoform showed significantly reduced neurite outgrowth as well as fewer emerging neurites. Furthermore, whole-cell patch-clamp recordings of patient-derived neuronal cells revealed a lower threshold for openings, indicating altered Na+ channel kinetics, suggesting a lower threshold for openings as compared to neuronal cells without the NFASC mutation. Taken together, our results suggest that loss of the full-length NFASC isoform NF186 causes perturbed neurogenesis and impaired neuronal biophysical properties resulting in a novel early-onset autosomal recessive ataxia syndrome.
Sugar Beet Micropropagation

ABSTRACT Vegetative in vitro multiplication is one of the most efficient methods for sugar beet (Beta vulgaris L.) propagation. The usual steps in this procedure are sterilization of explant, multiplication, rhizogenesis and acclimatization. In the paper is presented development of regeneration and multiplication techniques from different explants. It gives a detailed description of further micropropagation steps and presents necessary conditions for their realization. The paper also discuss different ways of micropropagation application especially in sugar beet breeding, but in other plant sciences as well.
Resurrection of a Global, Metagenomically Defined Microvirus

The Gokushovirinae (family Microviridae) are a group of single-stranded, circular DNA bacteriophages that have been detected in metagenomic datasets from every ecosystem on the planet. Despite their abundance, little is known about their biology or their bacterial hosts: isolates are exceedingly rare, known only from a very small number of obligate intracellular bacteria. By synthesizing circularized phage genomes from prophages embedded in diverse enteric bacteria, we produced viable gokushovirus phage particles that could reliably infect E. coli, thereby allowing experimental analysis of its life cycle and growth characteristics. Revived phages integrate into host genomes by hijacking a phylogenetically conserved chromosome-dimer resolution system, in a manner reminiscent of cholera phage CTX. Sequence motifs required for lysogeny are detectable in other metagenomically defined gokushoviruses, but we show that even partial motifs enable phages to persist in a state of pseudolysogeny by continuously producing viral progeny inside hosts without leading to collapse of their host culture. This ability to employ multiple, disparate survival strategies is likely key to the long-term persistence and global distribution of Gokushovirinae. The capacity to harness gokushoviruses as an experimentally tractable model system thus substantially changes our knowledge of the nature and biology of these ubiquitous phages.
Insight to the Mode of Action of Allium sativum Leaf Agglutinin (ASAL) Expressing in T 3 Rice Lines on Brown Planthopper

Brown planthopper, the sap sucking hemipteran pest, is one of the major contributors to the yield loss of rice through the world. To combat the situation researchers are interested identifying genes from plant origin having potentiality to develop hemipteran pest resistance. Interestingly, it was observed that rice plants expressing ASAL, a monocot mannose binding lectin, showed significant resistance to brown planthopper and green leafhopper. Additionally, antibiotic resistant marker gene free ASAL expressing rice lines were developed to overcome the biosafety issues. However, the basis behind the resistance against planthoppers is still not clearly understood. Ligand blot assay was performed with total BBMV protein from BPH and a ~56 kDa receptor protein was detected. LC MS/MS analysis revealed that the receptor protein is NADH quinone oxidoreductase (NQO), a key player in electron transport chain, insect defense response and male/female gametogenesis. Presumably interaction of ASAL with NQO may lead to toxicity and loss of fecundity among BPH feeding on ASAL expressing transgenic rice plants. These findings provide a stable scientific basis for considering these transgenic ASAL expressing rice plants as significant product for combating BPH attack associated yield loss of rice.
Multiple Timescales Account for Adaptive Responses across Sensory Cortices

Sensory systems encounter remarkably diverse stimuli in the external environment. Natural stimuli exhibit timescales and amplitudes of variation that span a wide range. Mechanisms of adaptation, a ubiquitous feature of sensory systems, allow for the accommodation of this range of scales. Are there common rules of adaptation across different sensory modalities? We measured the membrane potential responses of individual neurons in the visual, somatosensory, and auditory cortices of male and female mice to discrete, punctate stimuli delivered at a wide range of fixed and nonfixed frequencies. We find that the adaptive profile of the response is largely preserved across these three areas, exhibiting attenuation and responses to the cessation of stimulation, which are signatures of response to changes in stimulus statistics. We demonstrate that these adaptive responses can emerge from a simple model based on the integration of fixed filters operating over multiple time scales. SIGNIFICANCE STATEMENT Our recent sensations affect our current expectations and perceptions of the environment. Neural correlates of this process exist throughout the brain and are loosely termed adaptation. Adaptive processes have been described across sensory cortices, but direct comparisons of these processes have not been possible because paradigms have been tailored specifically for each modality. We developed a common stimulus set that was used to characterize adaptation in somatosensory, visual, and auditory cortex. We describe here the similarities and differences in adaptation across these cortical areas and demonstrate that adaptive responses may emerge from a set of static filters that operate over a broad range of timescales.
Self-reported sleep relates to hippocampal atrophy across the adult lifespan: results from the Lifebrain consortium

Abstract Objectives Poor sleep is associated with multiple age-related neurodegenerative and neuropsychiatric conditions. The hippocampus plays a special role in sleep and sleep-dependent cognition, and accelerated hippocampal atrophy is typically seen with higher age. Hence, it is critical to establish how the relationship between sleep and hippocampal volume loss unfolds across the adult lifespan. Methods Self-reported sleep measures and MRI-derived hippocampal volumes were obtained from 3105 cognitively normal participants (18–90 years) from major European brain studies in the Lifebrain consortium. Hippocampal volume change was estimated from 5116 MRIs from 1299 participants for whom longitudinal MRIs were available, followed up to 11 years with a mean interval of 3.3 years. Cross-sectional analyses were repeated in a sample of 21,390 participants from the UK Biobank. Results No cross-sectional sleep—hippocampal volume relationships were found. However, worse sleep quality, efficiency, problems, and daytime tiredness were related to greater hippocampal volume loss over time, with high scorers showing 0.22% greater annual loss than low scorers. The relationship between sleep and hippocampal atrophy did not vary across age. Simulations showed that the observed longitudinal effects were too small to be detected as age-interactions in the cross-sectional analyses. Conclusions Worse self-reported sleep is associated with higher rates of hippocampal volume decline across the adult lifespan. This suggests that sleep is relevant to understand individual differences in hippocampal atrophy, but limited effect sizes call for cautious interpretation.