Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of the presence and antibiotic resistance of Listeria species and aerobic mesophilic bacteria count of cow milks

Listeria species lead to mastitis infection in cows. The aerobic mesophilic bacteria count (total bacteria count) is one of the most important factors affecting udder health and determining the milk quality. The aim of this study was to determine the aerobic mesophilic bacteria count, one of the most important factors affecting cow's milk quality, and presence and the antibiotic resistance profiles of Listeria spp. , one of the factors causing mastitis in cows. As a result of isolation and identification for Listeria spp. , totally 3 L. monocytogenes (n: 68, 4.41%), 7 L. innocua (n: 68, 10.29%) and 3 L. ivanovii (n: 68, 4.41%) were isolated from cow milk samples. According to results of the disc diffusion method performed to determine antibiotic susceptibility, it was found that L. monocytogenes, L. innocua, and L. ivanovii isolates were susceptive against sulfamethoxazole/ trimethoprim, meropenem, vancomycin, streptomycin, oxacillin and erythromycin. The aerobic mesophilic bacteria in the cow milk samples were detected 1.1x107 cfu/ml as the highest and 2.3x102 cfu/ml as the lowest. The average aerobic mesophilic bacteria count of milk samples was calculated 256623.971 cfu/ml. The total bacteria (aerobic mesophilic bacteria) count (cfu/ml) of milk samples in the study was found to be high based on the criteria stated in the national and international standards. Also, Listeria species were isolated from these samples. Since intermediate and resistant Listeria species were determined against the antibiotics used as a treatment option in these isolates, it is thought that Listeria species should also be considered in mastitis infections in terms of etiology and treatment. It is considered that a national mastitis control program is needed for preventing the mastitis infections and antibiotic resistance development causing economic losses in dairy cattle enterprises in order to provide milking hygiene completely.
HIGH RESOLUTION DIGITAL 3D MODELLING OF SUBSURFACE MORPHOLOGICAL STRUCTURES OF KOUTOUKI CAVE, GREECE VISOKOLOČLJIVOSTNO DIGITALNO 3D MODELIRANJE PODZEMELJSKIH MORFOLOŠKIH STRUKTUR V JAMI KOUTOUKI, GRČIJA

Remote sensing techniques and laser scanning technology have given us the opportunity to study indoor environments, such as caves, with their complex and unique morphology. In the presented case study, we used a handheld laser scanner for acquiring points with projected coordinate information (X, Y, Z) covering the entire show cave of Koutouki; including its hidden passages and dark corners. The point cloud covers the floor, the walls, and the roof of the cave, as well as the stalactites, stalagmites and the connected columns that constitute the decoration of the cave. The absolute and exact placement of the point cloud within a geographic reference frame gives us the opportunity for three-dimensional measurements and detailed visualization of the subsurface structures. Using open - source software, we managed to make a quantification analysis of the terrain and generated morphological and geometric features of the speleothems. We identified 55 columns by using digital terrain analysis and processed them statistically in order to correlate them to the frame of the cave development. The parameters that derived are the contours, each column height, the speleothem geometry and volume, as well as the volume of the open space cavity. We argue that by the demonstrated methodology, it is possible to identify with high accuracy and detail: the geomorphological features of a cave, an estimate of the speleogenesis, and the abil-ity to monitor the evolution of a karstic system.
Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice

Meiosis is a hallmark event in germ cell development that accompanies sequential events executed by numerous molecules. Therefore, characterization of these factors is one of the best strategies to clarify the mechanism of meiosis. Here, we report tripartite motif-containing 41 (TRIM41), a ubiquitin ligase E3, as an essential factor for proper meiotic progression and fertility in male mice. Trim41 knockout (KO) spermatocytes exhibited synaptonemal complex protein 3 (SYCP3) overloading, especially on the X chromosome. Furthermore, mutant mice lacking the RING domain of TRIM41, required for the ubiquitin ligase E3 activity, phenocopied Trim41 KO mice. We then examined the behavior of mutant TRIM41 (ΔRING-TRIM41) and found that ΔRING-TRIM41 accumulated on the chromosome axes with overloaded SYCP3. This result suggested that TRIM41 exerts its function on the chromosome axes. Our study revealed that Trim41 is essential for preventing SYCP3 overloading, suggesting a TRIM41-mediated mechanism for regulating chromosome axis protein dynamics during male meiotic progression.
Publication practice in Taxonomy: Global inequalities and potential bias against negative results

There is broad recognition by practicing taxonomists that the field is going through a crisis, which has been dubbed the “taxonomic impediment”. There are many aspects involved in said crisis, but publication practices in taxonomy are often neglected or relegated to the backseat. We provide an initial foray into this topic via a worldwide survey with taxonomists, spanning all botanical and zoological groups, and career stages. Demographically, most of the respondents identified themselves as males (70%), working in Europe or North America (68%), in universities (50%) or museums (27%). Over half of the respondents are established/late-career researchers (only about 25% of full professors were female), with a low number of early-career researchers and graduate students (i.e., taxonomists in training). Nearly 61% of the men acquired their highest title at least eleven years ago, while only 41% of the women did so. Nearly 92% of the respondents have published new species descriptions, while around 60% and 26% have synonymized, respectively, species-level or subspecies-level taxa. In general, respondents perceive the act of describing new species to be easier than synonymizing species (p = 0.05). Established/late-career researchers and male researchers, particularly in Oceania and North America, found it easier to publish nomenclatural acts such as new species descriptions, while early-career researchers had their acts contested more often. Our results reaffirm the low academic recognition of the field, the lack of funding for research and publishing charges especially in the Global South, and the difficulty in finding specialized outlets (and the low impact factor of those journals) as persistent issues in taxonomy. Other significant problems raised by respondents include ethical issues in the peer-review process, a bias against newcomers in the field coming either from established researchers or committees, and taxonomic vandalism.
Effects of habitat homogenisation on assemblages associated with mussel clumps

Biodiversity loss is considered one of the main threats to marine ecosystems. In this framework of biodiversity decline, organisms that provide biogenic habitat play a relevant role by their capacity to structure assemblages and influence ecological processes. The Mediterranean mussel Mytilus galloprovincialis is considered an ecosystem engineer because it alters local environmental conditions maintaining habitat suitability for other organisms, and enhancing local biodiversity. Although it is widely recognized that mussel beds increase diversity, the drivers shaping these assemblages are poorly explored. We investigate whether mussel size homogenisation shapes the abundance, richness and structure of macrobenthic assemblages associated with mussel beds in two shores of the Galician coast (NW Spain). At each shore, two sites, 10 m apart, were selected and at each site, faunal assemblages were compared between mussel clumps showing shells of various sizes (control), and mussel clumps with closely similar-sized mussels, considered as homogenised. Homogenised clumps showed, in general, higher values in total number of individuals and species than control clumps. Regarding the effect of mussel size homogenisation on the multivariate structure of the assemblages, significant differences between control and homogenised clumps were found in three out of the four sites. Most relevant associated species usually reached higher abundances in homogenised clumps than control ones. Therefore, mussel size homogenisation influenced the structure of the macrofaunal assemblages associated with mussel beds but, its effect was context dependent (i.e., varied with sites). Information about the species contribution to dissimilarities among homogenised and control clumps was provided and the potential influence of sediment and algae on mussel clumps was discussed.
Systematic validation and assessment of immunohistochemical markers for central nervous system pathology in cetaceans, with emphasis on auditory pathways

Cetacean neuropathology is a developing field that aims to assess structural and neurochemical changes involved in neurodegenerative, infectious and traumatic processes, however markers used previously in cetaceans have rarely undergone systematic validation. This is a prerequisite to investigating the potential damage inflicted on the cetacean auditory system by anthropogenic noise. In order to assess apoptotic, neuroinflammatory and structural aberrations on a protein level, the baseline expression of biomarker proteins has to be characterized, implementing a systematic approach to validate the use of anti-human and anti-laboratory animal antibodies in dolphin tissues. This approach was taken to study 12 different antibodies associated with hypoxic-ischemic, inflammatory, plastic and excitatory-inhibitory changes implicated in acoustic trauma within the ventral cochlear nuclei and inferior colliculi of 20 bottlenose dolphins (Tursiops truncatus). Out of the 12 tested antibodies, pro-apoptotic protease factor 1 (Apaf-1), diacylglycerolkinase-ζ (DGK-ζ), B-cell lymphoma related protein 2 (Bcl-2), amyloid-β peptide (Aβ) and neurofilament 200 (NF200) were validated employing Western blot analyses and immunohistochemistry (IHC). The results of the validation process indicate specific patterns of immunoreactivity that are comparable to those reported in other mammals, thus suggesting a key panel of IHC biomarkers of pathological processes in the cetacean brain. As a consequence, the antibodies tested in this study may constitute a valid tool for supporting existing diagnostic methods in neurological diseases. The approach of systematic validation of IHC markers in cetaceans is proposed as a standard practice, in order for results to be transparent, reliable and comparable.
Posterior circulation ischemic stroke not involving the brainstem is associated with cardiovascular autonomic dysfunction

Ischemic stroke may induce cardiovascular autonomic dysfunction, but most previous studies have included patients with anterior circulation ischemic stroke or brainstem stroke. It remains unclear whether posterior circulation ischemic stroke (PCIS) without brainstem involvement also compromises cardiovascular autonomic modulation (CAM). Therefore, we aimed to assess CAM in PCIS patients with and without brainstem involvement.
Genomic Signatures of Divergent Ecological Strategies in a Recent Radiation of Neotropical Wild Cats

Abstract Ecological differentiation among diverging species is an important component of the evolutionary process and can be investigated in rapid and recent radiations. Here, we use whole genome sequences of five species from the genus Leopardus, a recently diversified Neotropical lineage with species bearing distinctive morphological, ecological, and behavioral features, to investigate genome-wide diversity, comparative demographic history and signatures of positive selection. Our results show that divergent ecological strategies are reflected in genomic features, for example a generalist species shows historically larger effective population size and higher heterozygosity than habitat specialists. The demographic history of these cats seems to have been jointly driven by climate fluctuations and habitat specialization, with different ecological adaptations leading to distinct trajectories. Finally, a gene involved in vertebrate retinal neurogenesis (POU4F2) was found to be under positive selection in the margay, a cat with notoriously large eyes that are likely associated with its nocturnal and arboreal specializations.
Advances in haploid embryonic stem cell research

Abstract Haploid embryonic stem cells are embryonic stem cells of a special type. Their nuclei contain one complete set of genetic material, and they are capable of self-renewal and differentiation. The emergence of haploid embryonic stem cells has aided research in functional genomics, genetic imprinting, parthenogenesis, genetic screening, and somatic cell nuclear transfer. This article reviews current issues in haploid stem cell research based on reports published in recent years and assesses the potential applications of these cells in somatic cell nuclear transfer, genome imprinting, and parthenogenesis. Summary Sentence Haploid embryonic stem cells represent a powerful tool for life science research that can be used in genetic screening, functional genomics, somatic cell nuclear transfer, parthenogenesis, and heterozygous cell research.
A performative view of knowledge exploitation and exploration : a case study of a higher education merger

---------------------------------------------------------------------------------------rganizational transformations, such as mergers and acquisitions, disrupt the steady state of organizational daily life. Under some conditions, these kinds of disruptions may actually alter the organizational and occupational structure of everyday work. However, current theories of organizational learning and knowledge governance, such as the so-called ‘knowledgeor capability-based view of the firm’, are inadequate when it comes to the potential number of structural variations inherent in an organizational transformation taking place in non-commercial organizational settings such as higher education institutions. In an exploratory case study of a university merger, this dissertation inductively examines how governance structures in universities impact the creation and exploitation of knowledge, both in core academic activities (research and teaching) and in related and supporting administrative tasks. This setting provides an institutional configuration that differs considerably from that which has informed most previous research on the creation, sharing and exploitation of knowledge, but in which there are prominent institutional locales for the governance of knowledge processes. Taking a practice lens, this study proposes a finer-grained picture of those structural variations by depicting the recursive relationship between changes in knowledge content (ostensive aspects) and knowledge-use practices (performative aspects) in the academic merger. Similarities and differences in relation to knowledge governance in firms are also identified. The findings suggest a classification of the micro-processes by which organizational and competence-based capabilities are recreated, improving our understanding of knowledge-based capabilities (re)creation at different levels of organization and through different stages of merger implementation. O
ZNF582 promoter methylation predicts cervical cancer radiosensitivity and ZNF582 protein overexpression reduces radiosensitivity by cell cycle arrest in S phase

ABSTRACT This study aimed to investigate the relationship between ZNF582 promoter methylation (ZNF582m ) level and radiosensitivity of cervical cancer and its biological basis. This was a prospective multicenter clinical study, comprising two independent cohorts of locally advanced cervical cancer patients. Exfoliated cervical cells were collected at 0, 24, 30, 36, 48, and 64 Gy to test ZNF582m levels. Radiotherapy response was evaluated according to RECIST Version. RT-PCR and WT were used to detect the mRNA and protein expression levels; MTT and flow cytometry were used to detect the cell viability and cell cycle, respectively. While clone formation and subcutaneous tumorigenesis in nude mice were used to detect the growth of HeLa cells with/without ZNF582 overexpression. In the first cohort, 22 cases achieved complete remission (CR) or partial response (PR), and the other 28 cases exhibited stable disease (SD). Radiotherapy reduced ZNF582m levels among all patients. Initial lever of ZNF582m was significantly higher in the Responder (CR + PR) group than in the SD group. Also, patients with higher initial lever ZNF582m were more sensitive towards radiotherapy than ZNF582m- low patients. The second cohort confirmed the above results. The amplitude of ZNF582m levels were related to the radiotherapeutic response; some patients of ZNF582m- low showed a transient increase in ZNF582m , and present greater radiosensitivity than other ZNF582m- low patients. In vitro, ZNF582 protein overexpression promoted cell cycle arrest in S phase. These results suggested that higher ZNF582m levels predicted greater radiosensitivity in clinical cervical cancer cases. Overexpressed ZNF582 conferred radioresistance by cell cycle arrest in vitro.
Strategies for Implementing Occupational eMental Health Interventions: Scoping Review

Background The implementation of eMental health interventions, especially in the workplace, is a complex process. Therefore, learning from existing implementation strategies is imperative to ensure improvements in the adoption, development, and scalability of occupational eMental health (OeMH) interventions. However, the implementation strategies used for these interventions are often undocumented or inadequately reported and have not been systematically gathered across implementations in a way that can serve as a much-needed guide for researchers. Objective The objective of this scoping review was to identify implementation strategies relevant to the uptake of OeMH interventions that target employees and detail the associated barriers and facilitation measures. Methods A scoping review was conducted. The descriptive synthesis was guided by the RE-AIM (reach, effectiveness, adoption, implementation, and maintenance) framework and the Consolidated Framework for Implementation Research. Results A total of 31 of 32,916 (0.09%) publications reporting the use of the web-, smartphone-, telephone-, and email-based OeMH interventions were included. In all, 98 implementation strategies, 114 barriers, and 131 facilitators were identified. The synthesis of barriers and facilitators produced 19 facilitation measures that provide initial recommendations for improving the implementation of OeMH interventions. Conclusions This scoping review represents one of the first steps in a research agenda aimed at improving the implementation of OeMH interventions by systematically selecting, shaping, evaluating, and reporting implementation strategies. There is a dire need for improved reporting of implementation strategies and combining common implementation frameworks with more technology-centric implementation frameworks to fully capture the complexities of eHealth implementation. Future research should investigate a wider range of common implementation outcomes for OeMH interventions that also focus on a wider set of common mental health problems in the workplace. This scoping review’s findings can be critically leveraged by discerning decision-makers to improve the reach, effectiveness, adoption, implementation, and maintenance of OeMH interventions.
SKI-INTERACTING PROTEIN interacts with SHOOT MERISTEMLESS to regulate shoot apical meristem formation

Abstract The shoot apical meristem (SAM), which is formed during embryogenesis, generates leaves, stems, and floral organs during the plant life cycle. SAM development is controlled by SHOOT MERISTEMLESS (STM), a conserved Class I KNOX transcription factor that interacts with another subclass homeodomain protein, BELL, to form a heterodimer, which regulates gene expression at the transcriptional level in Arabidopsis (Arabidopsis thaliana). Meanwhile, SKI-INTERACTING PROTEIN (SKIP), a conserved protein in eukaryotes, works as both a splicing factor and as a transcriptional regulator in plants to control gene expression at the transcriptional and posttranscriptional levels by interacting with distinct partners. Here, we show that, similar to plants with a loss of function of STM, a loss of function of SKIP or the specific knockout of SKIP in the SAM region resulted in failed SAM development and the inability of the mutants to complete their life cycle. In comparison, Arabidopsis mutants that expressed SKIP specifically in the SAM region formed a normal SAM and were able to generate a shoot system, including leaves and floral organs. Further analysis confirmed that SKIP interacts with STM in planta and that SKIP and STM regulate the expression of a similar set of genes by binding to their promoters. In addition, STM also interacts with EARLY FLOWERING 7 (ELF7), a component of Polymerase-Associated Factor 1 complex, and mutation in ELF7 exhibits similar SAM defects to that of STM and SKIP. This work identifies a component of the STM transcriptional complex and reveals the mechanism underlying SKIP-mediated SAM formation in Arabidopsis.
HR graduate employability: An exploration of organisational contributors

In the UK, Higher Education Institutions (HEIs) are striving to be more responsive to the needs of stakeholders, such as more closely aligning Higher Education (HE) with the work skills required by employers. From an academic standpoint, increasing attention is being paid to transference of ‘skills’ and other aspects of graduate employability from HE to the workplace. This paper outlines findings from a qualitative study into line managers’ perceptions and experiences of graduates working in graduate level jobs in the UK, placing particular emphasis on the influence of organisational contributors. The context of this research is focused on Human Resources (HR) graduates, a discipline that has seen little qualitative inquiry across the Higher Education (HE), employability and HR literature. As such, this paper works towards building up a richer ‘picture’ of HR graduate employability and HR graduate level work.
An updated patent review on monoamine oxidase (MAO) inhibitors

ABSTRACT Introduction Monoamine oxidase (MAO) inhibitors are currently used as antidepressants (selective MAO-A inhibitors) or as co-adjuvants for neurodegenerative diseases (selective MAO-B inhibitors). The research within this field is attracting attention due to their crucial role in the modulation of brain functions, mood, and cognitive activity, and monoamine catabolism. Areas covered MAO inhibitors (2018–2021) are discussed according to their chemotypes. Structure–activity relationships are derived for each chemical scaffold (propargylamines, chalcones, indoles, benzimidazoles, (iso)coumarins, (iso)benzofurans, xanthones, and tetralones), while the chemical entities were divided into newly synthesized molecules and natural metabolites. The mechanism of action and type of inhibition are also considered. Lastly, new therapeutic applications are reported, which demonstrates the clinical potential of these inhibitors as well as the possibility of repurposing existing drugs for a variety of diseases. Expert opinion MAO inhibitors here reported exhibit different potencies and isoform selectivity. These compounds are clinically licensed for multi-faceted neurodegenerative pathologies due to their ability to also act against other relevant targets (cholinesterases, inflammation, and oxidative stress). Moreover, the drug repurposing approach is an attractive strategy by which MAO inhibitors may be applied for the treatment of prostate cancer, inflammation, vertigo, and type 1 diabetes.