Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression and Refolding of an Anti-tumor Antibody AA98 Fragment V_H/κ

The functional fragment V H/κ of an anti tumor antibody AA98 was refolded in vitro by dilution refolding, gel filtration chromatography and urea gradient gel filtration, respectively. Concerning various factors in the renaturing buffer, such as GSH/GSSG ratio, arginine concentration, pH, flow rate, protein concentration in denaturing buffer and linear gradient, a urea gradient gel filtration method for V H/κ refolding was established. As a result, compared with the traditional dilution refolding and gel filtration, the activity recovery of V H/κ obtained by urea gradient gel filtration was significantly improved.
Interactive Effect of Boron and Salinity on Growth, Physiological and Biochemical Attributes of Wheat (Triticum aestivum)

A pot experiment was conducted to investigate the interactive effect of salinity (125 mM) and varying concentrations of boron (B) (2, 4 and 6 mM) on the growth and biochemical attributes of wheat. Results showed that application of 4 and 6 mM B along with 125 mM NaCl significantly declined plant biomass and leaf length. The concentration of B in plant leaves was significantly lower in treatments where salinity and B were applied together as compared to individual applications of 2, 4 and 6 mM B. The membrane permeability and protein concentration were significantly increased by the combined application of NaCl and B, whereas the chlorophyll pigments were not influenced. The phenolic compounds and ascorbic acid concentrations were reduced with the individual applied 6 mM B and when combined with application of 125 mM NaCl + 6 mM B. The concentration of Milondialdehyde (MDA) gradually increased by increasing B application and maximum was at the highest level of B and NaCl stress. It is concluded that salinity worsens the deteriorating effect of boron toxicity on wheat growth. © 2016 Friends Science Publishers
PCR-DGGE Analysis of Microbial Community in Shubat from Xinjiang

The microbial community structures of traditional fermented camel milk(shubat) from Xinjiang in China were analyzed using PCR-DGGE technique.Bacterial and yeast community exhibited 78%-84% and 80%-92% similarity among shubat samples from different regions,respectively.Through sequence analysis of V6-V8 region of 16S rDNA,the bacterial community in shubat was composed of Macrococcus caseolyticus,Lactobacillus helveticus,Lactobacillus brevis,Weissella hellenica,Lactobacillus sakei,Enterococcus durans,Enterococcus faecium,Leuconostoc lactis and so on.The yeast community was identified to consist of Kluyveromyces marxianus,Kazachstania unispora,Candida ethanolica and Geotrichum sp.by comparing sequence similarity with D1 region of 26S rDNA gene.These results will provide a theoretical foundation for developing culture starter of shubat.
Dental Restorative Digital Workflow: Digital Smile Design from Aesthetic to Function

Breakthroughs in technology have not been possible without influencing the medical sciences. Dentistry and dental materials have been fully involved in the technological and information technology evolution, so much so that they have revolutionized dental techniques. In this study, we want to create the first collection of articles on the use of digital techniques and software, such as Digital Smile Design. The aim is to collect all of the results regarding the use of this software, and to highlight the fields of use. Twenty-four articles have been included in the review, and the latter describes the use of Digital Smile Design and, in particular, the field of use. The study intends to be present which dental fields use “digitization”. Progress in this field is constant, and will be of increasing interest to dentistry by proposing a speed of treatment planning and a reliability of results. The digital workflow allows for rehabilitations that are reliable both from an aesthetic and functional point of view, as demonstrated in the review. From this study, the current field of use of Digital Smile Design techniques in the various branches of medicine and dentistry have emerged, as well as information about its reliability.
Preparation and stability of rumen protected γ-aminobutyric acid.

The study was conducted to develop a rumen protected γ-aminobutyric acid (GABA) with low ruminal degradability and high post-ruminal dissolution.GABA content,coating material and pressure were used as variables in a L9(34) orthogonal design to select samples with high insoluble rate,then ruminal degradability and post-ruminal dissolution were determined.The results showed as follows:as coating material,solid palm oil product was superior to the mixture of solid palm oil and glyceryl monostearate.The sample produced under the condition of 50% GABA,encapsulated with solid palm oil and under the pressure of 11 kPa was degraded at least in rumen.Its utilization efficiency was 75.8%. The sample produced under the conditions of GABA content of 50%,encapsulated with solid palm oil and glyceryl monostearate equal mass mixture and under the pressure of pressure 9 kPa presented the highest post-ruminal dissolution rate.It is suggested that the solid palm oil can be used as coating material to produced rumen protected GABA with low ruminal degradability and high post-ruminal dissolution.
Establishment and Application of RT-PCR for Detection of Canine Distemper Virus

Two pairs of PCR primers were designed according to the nucleocapsid protein sequences of canine distemper virus(CDV) in GenBank.A RT-PCR method was established.The result showed the expected products of 335 bp length could be amplified from RNA of CDV vaccine and suspected cases.The results were examined by electrophoresis.The RT-PCR assay of CDV was proved to be specific and sensitive.It is method used for rapid diagnosis of CDV.
Determination of triglyceride in lipoproteins separated by agarose gel electrophoresis and its clinical application

A simple method was developed for measurement of triglyceride(Tg)in lipoproteins. Lipoproteins separated by agarose gel electrophoresis.The Tg of bands was stained by specific enzymatic method(GPOTrinder)after electrophoresis.The relative amounts of Tg in lipoproteins were determined by densitometric scanning at 520 nm. Absolute triglyceride concentrations of the respective lipoprotein fractions were calculated from total Tg. Comparison of the electrophoresis method with a combined ultracentrifugation/precipitation method in 24 serum resulted in following correlation coefficients:HDLTg:=0873;VLDLTg:=0955;LDLTG:=0912The intraassay and interassay coefficients of variation were:8010238654370%;9516268854897%The average recovery was 888%934%917% respectively.This method is easy to perform,and is a precise and accurate technique for the determination of lipoprotein Tg. The results of 116 control subjects and 60 coronary heart disease(CHD)patients were shown:Total TGLDLTGLDLTG/LDLCH were valuable characler
In-Vitro Antioxidant Properties of the Leaf Extract of Mimosa pudica Linn

Objective: The antioxidants recovered from medicinal plants were widely used in the treatment of disease. Therefore, we aimed to investigates the antioxidant potentials of leaf extract of Mimosa pudica Linn. Methods: The plant extract was collected by using the hexane solvent and the crude extract was collected by vacuum distillation. In vitro antioxidant activities of the extract were exerted by evaluating 2,2-Diphenyl-Picrylhydrazyl (DPPH), hydroxyl, nitric oxide and superoxide radicals, in addition the total phenolic content also measured. Results: The hexane extracts from Mimosa pudica Linn. revealed significant scavenging effect on DPPH (IC5020.83 mM), hydroxyl (IC5019.37 mM), nitric oxide (IC5021.62 mM) and superoxide (IC50 22.19 mM) radicals. Conclusion: The antioxidant activities exhibited by M. pudica could be used for the treatment of various disorders. Further studies are needed in this direction.
Characterization and Photoprotector Activity of Endophytic Fungal Pigments from Coastal Plant Sarang Semut (Hydnophytum formicarum)

Endophytic fungus RS3 isolated from coastal plant sarang semut (Hydnophytum formicarum) produced extracellular black pigment. The aims of this research were to obtain the pigment, to characterize and to determine the photoprotector activity. This research was conducted into several steps, that were determination of the best precipitating agent, characterization using instrument and solubility analysis, and analysis of Sun Protection Factor (SPF). Results showed the pigment was precipitated using acid solvent with pH ≤ 2.5. Functional groups of pigment were hydroxyl, aromatic ring, phenol and amine. According to its characteristics, black pigment produced by RS3 isolate was proposed as melanin. The photoprotector analysis showed the SPF value was 11.33.
Uptake Kinetics of Different Nitrogen Forms by Chinese Kale

ABSTRACT In this paper, the uptake kinetics of various nitrogens (nitrate (NO3−), ammonium (NH4+), urea, amino acid) by Chinese kale (Brassica oleracea L. var. Bailey) were studied under hydroponic condition. The results indicated that the uptake kinetics of organic and inorganic nitrogen (N) by Chinese kale conform to the Michaelis–Menten equation, and the maximum uptake rate (Vmax) and affinity index (1/Km) showed nitrate (NO3—N) > ammonium (NH4+-N) > urea-N > Gly-N, with significant differences between treatments (p < 0.05). Adding different types of N to NO3− nutrient solution had little impact on its affinity, but significantly decreased the NO3− Vmax, which showed NO3–N > NO3− + NH4+ > NO3− + urea > NO3− + Gly. Chinese kale preferred inorganic N to organic N, with NO3− preceding NH4+. Adding organic and NH4+ N to nutrient solution reduced the NO3− uptake capacity by the plant.
MEMETSKI ALGORITEM ZA SKLEPANJE O KONTEKSTNO NEODVISNIH GRAMATIKAH IN NJEGOVA UPORABA PRI NAČRTOVANJU DOMENSKO SPECIFIČNEGA JEZIKA

V doktorski disertaciji predstavimo memetski algoritem za sklepanje o kontekstno neodvisnih gramatikah MAGIc. Memetski algoritem predstavlja evolucijski algoritem z vkljucenim operatorjem lokalnega iskanja. Sklepanje o gramatikah spada na podrocje strojnega ucenja in predstavlja družino metod, ki na podlagi pozitivnih in opcijsko negativnih vhodnih primerov doloci strukturo oz. izpelje gramatiko. Algoritem MAGIc sklepa o kontekstno neodvisnih gramatikah samo na podlagi pozitivnih primerov, ceprav lahko v postopek sklepanja vkljucimo tudi negativne primere. Pri sklepanju uporablja kot dodatno znanje razlike med primeri, katere uporabi za razsiritev obstojecih gramatik, da razpoznajo vec vhodnih primerov. Algoritem smo testirali na množici domensko specificnih jezikov, napravili primerjavo z algoritmom TBL in ga razsirili z možnostjo vkljucevanja nove sintakse v obstojeco gramatiko splosnonamenskega jezika. Algoritem se je izkazal kot uspesen za sklepanje o kontekstno neodvisnih gramatikah domensko specificnih jezikov in je neodvisen od zaporedja vhodnih primerov. Kljucna operatorja algoritma sta lokalno iskanje in generalizacija.
Preliminary Screening for Resistant Soyben Cultivars to Powdery Mildew in Southern China

For the first time powdery mildew resistant was screened in seven provinces of southern cultivated soybean germplasm in order to obtain resistant soybean resources for providing excellent foster parents in further cross-breeding of resistant varieties. Furthermore,the distribution of resources cultivated soybean disease and prevention and control of powdery mildew were discussed in this study. Test materials from Hainan,Guangdong,Hunan,Guangxi,Fujian,Sichuan and Jiangxi provinces( regions) were used. The incidence survey was conducted for 3 times when the plants were fully incidence,at the same time,spray inoculation method was used. There were 161 parts in 285 parts which showed antiviral response accounting for56. 5% of total resources identified; 33 were intermediate reaction types,accounting for 11. 6%; 91 resource plant showed susceptible reaction,accounting for 31. 9%. It showed that cultivated soybeans which owned resistant ability were the most abundant in Guangxi,accounting for 78. 6%; followed by Guangdong and Sichuan,resistance rates were 75. 0% and 64. 5%.
Effects of foetal ethanol exposure on hypothalamic-pituitary adrenal axis function and behaviour on the elevated plus maze

This thesis investigated effects of prenatal ethanol exposure on hypothalamicpituitary-adrenal (HPA) axis function and on behaviour on the elevated plus maze (+maze). Male and female Sprague-Dawley rats from prenatal ethanol (E), pair-fed (PF) and ad lib-fed control (C) treatment groups, were tested in adulthood in all studies. The hormonal studies investigated the hypothesis that a deficit in feedback inhibition of the H P A axis may underlie the hormonal hyperresponsiveness seen in E rats. The effects of dexamethasone (DEX) blockade on corticosterone (CORT) levels and adrenocorticotrophin (ACTH) levels were examined over a 36 hour (h) period. Following D E X , E males and females had significantly higher stress CORT levels and/or A C T H than PF and C animals, and showed differential responsiveness following D E X administration depending upon the time of day tested. At the trough of the circadian rhythm, E males did not differ from PF and C males, whereas E females had increased stress CORT levels compared to PF and C females. In contrast, at the peak of the circadian rhythm, E males showed increased stress CORT levels but not A C T H , whereas E females showed increased stress CORT and A C T H levels. These datasupport the hypothesis that E animals may exhibit deficits in HPA feedback inhibition. Two other possible mechanisms for HPA hyperresponsiveness were investigated during the trough of the CORT circadian cycle. First, adrenal sensitivity to exogenous A C T H was examined. No significant differences were found among prenatal treatment
Impact of the Amenity Value of Urban Green Space on the Price of House in Shanghai

The basic green infrastructure in urban areas,urban green space typically performs important ecological and socio-economic functions.It is the most important spatial type in the urban planning construction.As the spatial base of the harmony between human and nature in urban area,urban green space is a spatial adjuster that improves the structure and function of urban system and is viewed as an important spatial carrier to guarantee the sustainability of urban development. In this study,Shanghai city was chosen as a case.Based on 689 resident samples,by the support of spatial analysis function of GIS,the amenity value of urban green space was estimated through twenty three variables out of four types of factors(house structure,accessibility,area,landscape metrics) using Hedonic Price Model(HPM). The results showed that:(1) urban green space had amenity value,and the spatial distribution,size and types had significant influence on the price of house,but the influence was different;(2) the residents had the trend to live close to green space,and then there was a ring social pattern around the large green space;(3) the influence of landscape metrics on house price indicated that the pattern of green space was also an important factor;(4)the characters of house structure such as size,age are the basically internal factors,while the extrinsic factors comprise the time to urban center,to the nearest hospital,college,Metro station,in addition to urban green spaces.The amenity value analysis on green space can put forward useful information and reference which can be used for the urban green space plan and urban development strategy plan by urban planners and policy-makers.
Key Point Detection in 3D Reconstruction Based On Human- Computer Interaction

Aiming at solving problem of points’ redundancy caused by full automatically detecting points and the problem of large workload caused by picking all points manually, I advanced a new method of picking points which is based on Human-Computer Interaction in our 3D reconstruction platform after automatically detecting points. We first detected and matched points automatically and got the homograph matrix between two images, then projected points which were picked by hand on the one image to the other image, at last we would search the interesting feature points in the neighborhood of corresponding points in the two images. Experiments have shown that this method decreases the redundancy brought by large number of points and successfully finds the important feature points, so it lays a good foundation for 3D reconstruction.
Studies on the Gonococcus I.

In previous papers I have called attention to lysis in the gonococcus and have emphasized this phenomenon as being invariably present to a greater or less degree in artificial culture. This study was undertaken with the objects of ascertaining which factors bring about and influence the lytic changes? whether the process is due wholly to the action of enzymes, as is commonly maintained, or to other causes? and of obtaining a more intimate knowledge of the biochemistry of the organisms. This has seemed to me important in view of the practical value such observations might have in the problem of immunity. The material consisted of twenty-two strains of gonococcus isolated by me within the past year from cases of gonorrhea in men and young girls, in addition to several, including six strains of meningococcus, kindly given me by other workers. The question first to be considered was whether lysis might be due to conditions in the culture media, or to causes within the organisms themselves. Accordingly the strains were grown upon all media specially recommended for cultivating the gonococcus, with the result that all strains showed lysis upon all media,1 varying only in degree under conditions to be mentioned later. This fact being determined there remained to be shown what element of the media, common to all, initiated the lysis, on the assumption that the conditions of environment were alone responsible.
Study on Cardiotoxicity and Toxic Substance of Toad Venom in Isolated Guinea Pig Heart

Objective:To study toad venom(BV)-induced cardiac toxicity and the toxic substances in isolated guinea-pig heart.Method:Isolated guinea pig hearts were switched to the Langendorff mode and perfused with or without BV.Measure the content of toad venom in perfused hearts using HPLC.Result:BV caused auriculo-ventricular block,supraventicular tachycardia,ventricular tachycardia,ventricular fibrillation and other arrhythmias phenomenon in isolated hearts.The cumulative doses of bufadienolides(60±11.5)μg induced cardiac arrest.Nine kinds of bufadienolides were detected by HPLC in the isolated hearts.Compare their affinity with the hearts:arenobufaginbufalintelocinobufaginhellebrigeningamabufotalincinobufalinbufotalinresibufogenindesacety-bufotalin.Conclusion:BV has cardiotoxicity on the guinea pig heart and the material basis of toad induced toxic effects may be gamabufotalin,arenobufagin and so on.
Expression and identification of the subsection from pAPN in insect cells expression systems

To investigate which part of porcine aminopeptidase(pAPN) is the functional domain of receptor for PEDV,we removed the signal peptide and divided pAPN into three sections: SPA,SPB and SPC.Then we use a pair of isocaudarner Xhol I and Sal I to connect the signal peptide and SPA,SPB and SPC respectively and connected to pFastBacTM1 finally.We express the SPA,SPB and SPC by Bac-to-Bac baculovirus expression systems and get the secreted protein 42,56 ku as we expected by SDS-PAGE.The result of western blot shows that SPA,SPB and SPC can specific binding to the nature antiserum of pAPN.It shows that we have get the three parts of pAPN successfully and prepared for the futher research on the functional domain of pAPN.
TETRAPLOID INDUCTION IN PATINOPECTEN YESSOENSIS WITH CHEMICALS

The scallop (Patinopecten yessoensis) were released and cultured in north China sea transplanted from Japan since 1980. Tetraploid veliger were carried out with four chemicals on this scallop in 1998-2000. Tetraploid were induced by inhibition of the polar body and first mitosis with cytoplalasin B (CB), colchicine, 6-DMAP and caffeine combined with CB, respectively. It indicated that the yield of tetraploidy not more than 25% induced by inhibiting the first mitosis with CB, colchicine and 6-DMAP, respectively, while it can be induced with higher percentage by blocking PB_(1) releasing with CB. The tetraploidy percentage of groups shock began at 15-22min after fertilization are h igher than that shock began at 42min, and the yield of tetraploidy also increased with the CB density and time lasting. The highest tetraploid percentage at veliger larvae stage is 56.5%, shocked with CB after 20-22 to 62-67 minutes after fertilization at 12deg C. Development speed of shocked group embryo was 48 hours later than control to veliger, there are a lot of trochophore in shocked groups after fertilization which chromosome are 21-28.
An Improved Denoising Method Based on Stationary Wavelet Transform

Abstract. It is quite difficult to analyze experimental signals since they have low Signal-to-Noise Ratio (SNR). Discrete Stationary Wavelet Transform (SWT) can be used for signal denoising because of its energy concentration and shift invariance feature. this paper focuses on the noise reduction algorithms based on SWT and proposed a new threshold function for better denoising effect. The method is experimentally evaluated and simulated. The result shows that the proposed method is an effective tool for signal denoising.
Unbiased Smoothing using Particle Independent Metropolis-Hastings

We consider the approximation of expectations with respect to the distribution of a latent Markov process given noisy measurements. This is known as the smoothing problem and is often approached with particle and Markov chain Monte Carlo (MCMC) methods. These methods provide consistent but biased estimators when run for a finite time. We propose a simple way of coupling two MCMC chains built using Particle Independent Metropolis–Hastings (PIMH) to produce unbiased smoothing estimators. Unbiased estimators are appealing in the context of parallel computing, and facilitate the construction of confidence intervals. The proposed scheme only requires access to off-the-shelf Particle Filters (PF) and is thus easier to implement than recently proposed unbiased smoothers. The approach is demonstrated on a Levy-driven stochastic volatility model and a stochastic kinetic model.
Supervised Spatial Transformer Networks for Attention Learning in Fine-grained Action Recognition

We aim to propose more effective attentional regions that can help develop better fine-grained action recognition algorithms. On the basis of the spatial transformer networks’ capability that implements spatial manipulation inside the networks, we propose an extension model, the Supervised Spatial Transformer Networks (SSTNs). This network model can supervise the spatial transformers to capture the regions same as hard-coded attentional regions of certain scale levels at first. Then such supervision can be turned off, and the network model will adjust the region learning in terms of location and scale. The adjustment is conditioned to classification loss so that it is actually optimized for better recognition results. With this model, we are able to capture attentional regions of different levels within the networks. To evaluate SSTNs, we construct a six-stream SSTN model that exploits spatial and temporal information corresponding to three levels (general, middle and detail). The results show that the deep-learned attentional regions captured by SSTNs outperform hard-coded attentional regions. Also, the features learned by different streams of SSTNs are complementary to each other and better result is obtained by fusing the features.