Miracle-dz/UniFAR-FTUs huggingface dataset:
Optimization of tourism routes in Lushunkou District based on ArcGIS

With the advancements and developments in China’s tourism industry, various autonomous forms of tourism have been gaining prominence. As such, to facilitate tourists and provide them with maximum experience while economizing on time and cost is essential. One approach toward achieving this is the optimization of tourism routes. However, so far the studies on this approach have focused primarily on inland tourist sites and have lacked a geographic perspective. Therefore, this study undertook the tourism resource data of Lushunkou District of 2020, used the ArcGIS accessibility evaluation model to analyze tourism resources, and finally used the Vehicle Routing Problem of network analysis technology to optimize the tourism route of Lushunkou District and obtain the general overall intellectual framework and technical methods for tourism route optimization. The results showed that the ArcGIS accessibility evaluation model could be used to integrate resources in the tourism area before using the Vehicle Routing Problem to optimize the analysis of tourism routes, thereby enabling the separation of different types of tourism. These divisions were based on the Vehicle Routing Problem to optimize routes for one-day and two-day tours. A new method and model for optimization for tourism routes was constructed to provide a basis and reference for the optimization of tourism routes in similar cities. The observations and results of the present study can facilitate the government in developing the tourism industry and maximizing the benefits obtained from them. Further, travel agencies and tourists will have the provision of designing optimum tourism routes.
Hospitalization costs of coronaviruses diseases in upper-middle-income countries: A systematic review

Background COVID-19, SARS and MERS are diseases that present an important health burden worldwide. This situation demands resource allocation to the healthcare system, affecting especially middle- and low-income countries. Thus, identifying the main cost drivers is relevant to optimize patient care and resource allocation. Objective To systematically identify and summarize the current status of knowledge on direct medical hospitalization costs of SARS, MERS, or COVID-19 in Upper-Middle-Income Countries. Methods We conducted a systematic review across seven key databases (PubMed, EMBASE, BVS Portal, CINAHL, CRD library, MedRxiv and Research Square) from database inception to February 2021. Costs extracted were converted into 2021 International Dollars using the Purchasing Power Parity-adjusted. The assessment of quality was based on the protocol by the BMJ and CHEERS. PROSPERO 2020: CRD42020225757. Results No eligible study about SARS or MERS was recovered. For COVID-19, five studies presented cost analysis performed in Brazil, China, Iran, and Turkey. Regarding total direct medical costs, the lowest cost per patient at ward was observed in Turkey ($900.08), while the highest in Brazil ($5,093.38). At ICU, the lowest was in Turkey ($2,984.78), while the highest was in China ($52,432.87). Service care was the most expressive (58% to 88%) cost driver of COVID-19 patients at ward. At ICU, there was no consensus between service care (54% to 87%) and treatment (72% to 81%) as key burdens of total cost. Conclusion Our findings elucidate the importance of COVID-19 on health-economic outcomes. The marked heterogeneity among studies leaded to substantially different results and made challenging the comparison of data to estimate pooled results for single countries or regions. Further studies concerning cost estimates from standardized analysis may provide clearer data for a more substantial analysis. This may help care providers and policy makers to organize care for patients in the most efficient way.
A secretory phospholipase A2 of a fungal pathogen contributes to lipid droplet homeostasis, assimilation of insect‐derived lipids, and repression of host immune responses

Secretory phospholipase A2s (sPLA2s) are found in a wide range of organisms from bacteria to higher plants and animals and are involved in varied and cellular processes. However, roles of these enzymes in microbial pathogens remain unclear. Here, an sPLA2 (BbPLA2) was characterized in the filamentous insect pathogenic fungus, Beauveria bassiana. BbPLA2 was exclusively expressed in insect hemolymph‐derived cells (hyphal bodies), and its expression was induced by insect‐derived nutrients and lipids, and nutrient starvation. High levels of secretion of BbPLA2 were observed as well as its distribution in hyphal body lipid drops (LDs). Overexpression of BbPLA2 increased the ability of B. bassiana to utilize insect‐derived nutrients and lipids, and promoted LD accumulation, indicating functions for BbPLA2 in mediating LD homeostasis and assimilation of insect‐derived lipids. Strains overexpressing BbPLA2 showed moderately increased virulence, including more efficient penetration of the insect cuticle and evasion of host immune responses as compared to the wild type strain. In addition, B. bassiana‐activated host immune genes were downregulated in the BbPLA2 overexpression strain, but upregulated by infections with a ΔBbPLA2 strain. These data demonstrate that BbPLA2 contributes to LD homeostasis, assimilation of insect‐derived lipids, and repression of host immune responses.
Quantitative Lipidomics and Spatial MS-Imaging Uncovered Neurological and Systemic Lipid Metabolic Pathways Underlying Troglomorphic Adaptations in Cave-Dwelling Fish

Abstract Sinocyclocheilus represents a rare, freshwater teleost genus endemic to China that comprises the river-dwelling surface fish and the cave-dwelling cavefish. Using a combinatorial approach of quantitative lipidomics and mass-spectrometry imaging (MSI), we demonstrated that neural compartmentalization of lipid distribution and lipid metabolism is associated with the evolution of troglomorphic traits in Sinocyclocheilus. Attenuated docosahexaenoic acid (DHA) biosynthesis via the Δ4 desaturase pathway led to reductions in DHA-phospholipids in cavefish cerebellum. Instead, cavefish accumulates arachidonic acid-phospholipids that may disfavor retinotectal arbor growth. Importantly, MSI of sulfatides coupled with immunostaining of myelin basic protein and transmission electron microscopy images of hindbrain axons revealed demyelination in cavefish raphe serotonergic neurons. Demyelination in cavefish parallels the loss of neuroplasticity governing social behavior such as aggressive dominance. Outside the brain, quantitative lipidomics and qRT-PCR revealed systemic reductions in membrane esterified DHAs in the liver, attributed to suppression of genes along the Sprecher pathway (elovl2, elovl5, and acox1). Development of fatty livers was observed in cavefish; likely mediated by an impeded mobilization of storage lipids, as evident in the diminished expressions of pnpla2, lipea, lipeb, dagla, and mgll; and suppressed β-oxidation of fatty acyls via both mitochondria and peroxisomes as reflected in the reduced expressions of cpt1ab, hadhaa, cpt2, decr1, and acox1. These neurological and systemic metabolic adaptations serve to reduce energy expenditure, forming the basis of recessive evolution that eliminates nonessential morphological and behavioral traits and giving cavefish a selective advantage to thrive in caves where proper resource allocation becomes a major determinant of survival.
Suppression of Sost/Sclerostin and Dickkopf‐1 Augment Intervertebral Disc Structure in Mice

Intervertebral disc (IVD) degeneration is a leading cause of low back pain, characterized by accelerated extracellular matrix breakdown and IVD height loss, but there is no approved pharmacological therapeutic. Deletion of Wnt ligand competitor Lrp5 induces IVD degeneration, suggesting that Wnt signaling is essential for IVD homeostasis. Therefore, the IVD may respond to neutralization of Wnt ligand competitors sost(gene)/sclerostin(protein) and/or dickkopf‐1 (dkk1). Anti‐sclerostin antibody (scl‐Ab) is an FDA‐approved bone therapeutic that activates Wnt signaling. We aimed to (i) determine if pharmacological neutralization of sclerostin, dkk1, or their combination would stimulate Wnt signaling and augment IVD structure and (ii) determine the prolonged adaptation of the IVD to global, persistent deletion of sost. Nine‐week‐old C57Bl/6J female mice (n = 6–7/group) were subcutaneously injected 2×/week for 5.5 weeks with scl‐Ab (25 mg/kg), dkk1‐Ab (25 mg/kg), 3:1 scl‐Ab/dkk1‐Ab (18.75:6.25 mg/kg), or vehicle (veh). Separately, IVD of sost KO and wild‐type (WT) mice (n = 8/group) were harvested at 16 weeks of age. First, compared with vehicle, injection of scl‐Ab, dkk1‐Ab, and 3:1 scl‐Ab/dkk1‐Ab similarly increased lumbar IVD height and β‐catenin gene expression. Despite these similarities, only injection of scl‐Ab alone strengthened IVD mechanical properties and decreased heat shock protein gene expressions. Genetically and compared with WT, sost KO enlarged IVD height, increased proteoglycan staining, and imbibed IVD hydration. Notably, persistent deletion of sost was compensated by upregulation of dkk1, which consequently reduced the cell nuclear fraction for Wnt signaling co‐transcription factor β‐catenin in the IVD. Lastly, RNA‐sequencing pathway analysis confirmed the compensatory suppression of Wnt signaling and revealed a reduction of cellular stress‐related pathways. Together, suppression of sost/sclerostin or dkk1 each augmented IVD structure by stimulating Wnt signaling, but scl‐Ab outperformed dkk1‐Ab in strengthening the IVD. Ultimately, postmenopausal women prescribed scl‐Ab injections to prevent vertebral fracture may also benefit from a restoration of IVD height and health. © 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
A carvacrol‐based product reduces Campylobacter jejuni load and alters microbiota composition in the caeca of chickens

This study was conducted to test the ability of a carvacrol‐based formulation (Phodé, France) to decrease the C. jejuni caecal load in inoculated broiler chickens and to study the impact of the C. jejuni inoculation alone or combined with the product, on the caecal microbiota.
Emergence of Direction-Selective Retinal Cell Types in Task-Optimized Deep Learning Models

Convolutional neural networks (CNNs), a class of deep learning models, have experienced recent success in modeling sensory cortices and retinal circuits through optimizing performance on machine learning tasks, otherwise known as task optimization. Previous research has shown task-optimized CNNs to be capable of providing explanations as to why the retina efficiently encodes natural stimuli and how certain retinal cell types are involved in efficient encoding. In our work, we sought to use task-optimized CNNs as a means of explaining computational mechanisms responsible for motion-selective retinal circuits. We designed a biologically constrained CNN and optimized its performance on a motion-classification task. We drew inspiration from psychophysics, deep learning, and systems neuroscience literature to develop a toolbox of methods to reverse engineer the computational mechanisms learned in our model. Through reverse engineering our model, we proposed a computational mechanism in which direction-selective ganglion cells and starburst amacrine cells, both experimentally observed retinal cell types, emerge in our model to discriminate among moving stimuli. This emergence suggests that direction-selective circuits in the retina are ecologically designed to robustly discriminate among moving stimuli. Our results and methods also provide a framework for how to build more interpretable deep learning models and how to understand them.
Cardiorespiratory fitness and the incidence of coronary surgery and postoperative mortality: the HUNT study

Abstract OBJECTIVES Low physical activity and cardiorespiratory fitness are known risk factors for coronary artery disease, but how they affect the risk of undergoing coronary artery bypass graft surgery is not established. We explored how physical activity and estimated cardiorespiratory fitness affect the risk of coronary surgery and postoperative outcome. METHODS Participants with no history of coronary disease from the second wave of the Trøndelag Health Study (HUNT2) were cross-linked with the local heart surgery register and the Norwegian Cause of Death Registry. Cardiorespiratory fitness was estimated by a previously developed algorithm using clinical and self-reported information. Fine-Gray competing risk analyses were used to calculate the risk of undergoing isolated coronary surgery across physical activity groups and estimated cardiorespiratory fitness (mL/kg/min) as quintiles and per 1 metabolic equivalent of task (MET) (3.5 mL/kg/min). RESULTS We included 45,491 participants. The mean population age was 46.0 [standard deviation (SD) 15.8] years, and the mean estimated fitness was 41.3 (SD 8.9) mL/kg/min. A total of 672 (1.5%) participants underwent coronary surgery during the follow-up period. The risk of undergoing isolated coronary surgery was 26% [95% confidence interval (CI) 3–44] lower for those classified as highly active compared to those classified as least active. Further, an 11% (95% CI 6–15) lower risk per 1-MET (3.5 mL/kg/min) of higher fitness. Finally, we observed a 15% (95% CI 5–23) lower mortality risk after surgery per 1-MET of higher fitness among those undergoing surgery. CONCLUSIONS High levels of physical activity and high estimated fitness levels were inversely associated with the risk of developing coronary disease requiring surgery and overall mortality after surgery.
Remodeling Yersinia pseudotuberculosis to generate a highly immunogenic outer membrane vesicle vaccine against pneumonic plague

Significance Yersinia pestis, the etiologic agent of plague, has been responsible for high mortality in several epidemics throughout human history. This plague bacillus has been used as a biological weapon during human history and is currently one of the deadliest biological threats. Currently, no licensed plague vaccines are available in the Western world. Since an array of immunogens are enclosed in outer membrane vesicles (OMVs), immune responses elicited by OMVs against a diverse range of antigens may reduce the likelihood of antigen circumvention. Therefore, self-adjuvanting OMVs from a remodeled Yersinia pseudotuberculosis strain as a type of plague vaccine could diversify prophylactic choices and solve current vaccine limitations.
Hypochlorous acid as a disinfectant for high‐risk HPV: Insight into the mechanism of action

Medical instruments that are not autoclavable but may become contaminated with high‐risk human papillomaviruses (HPVs) during use must be thoroughly disinfected to avoid the possibility of iatrogenic transmission of infection. There is an expectation that prolonged soaking of instruments in the United States Food and Drug Administration‐cleared chemical disinfectant solutions will result in high‐level decontamination, but HPV16 and HPV18 are known to be resistant to commonly used formulations. However, they are susceptible to a variety of oxidative agents, including those based on chlorine. Here, we tested the efficacy of homogeneous hypochlorous acid (HOCl) solutions against mature infectious virions of HPV16 and HPV18 dried onto butadiene styrene coupons and ultrasonic probes. Both viruses were inactivated to >4 log reduction value (LRV) after 15 s on coupons and 5 min on ultrasonic probes. Morphologic changes became evident within those contact times by transmission electron microscopy when HPV16 virus‐like particles were exposed to HOCl under identical conditions. Mass spectrometry analysis of trypsin‐digested products of L1 capsid proteins exposed to HOCl showed that mostly conserved residues were modified by oxidation and that these changes rapidly lead to instability of the protein demonstrable on sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE). Modifications to these residues may contribute to rapid virus inactivation. The use of homogeneous HOCl solutions for HPV decontamination provides a highly effective means of assuring the safety of nonautoclavable medical instruments.
Time to change direction in training load monitoring in elite football? The application of MEMS accelerometers for the evaluation of movement requirements

ABSTRACT In elite football, the emphasis is placed on monitoring the output from the Global Positioning Systems (GPS) component of a Micro-Electro-Mechanical Systems (MEMS) device; however, this does not comprehensively overview the total demands due to the intermittent multidirectional nature. The aim of the study was to investigate the application of accelerometer data provided by MEMS, to evaluate movement requirements in elite football. A two-staged research approach, involving an effectiveness and efficacy stage, was deployed. The effectiveness stage examined two MEMS-accelerometer variables (PlayerLoadTM (PL) and PlayerLoadTM per meter (PL.m−1)) for four years and four months. Ninety-nine English Premier League outfield football players’ participated. In the efficacy stage, 26 elite outfield football players completed three different training modalities (running, possession and dribbling) and a range of MEMS-accelerometer variables were analysed. In the effectiveness stage, the mean difference in PL for all training activities other than Set Pieces were similar to Matches (−283 to −246au). Model coefficients for PL.m−1 were smallest in Team Shape (−0.00114au), Attacking (0.00025au) and Games (0.00196au), and largest for Possession (0.03356AU), Defending (0.03182au) and Skills Games (0.03106au) compared to Matches. The findings suggest that PL.m−1 but not PL may be effective at describing differences in movement requirements. In the efficacy stage, PL.m−1 and inertial movement analysis (IMA) efforts were the only variables that had greater mean differences in the smaller conditions, confirming PL.m−1’s suitability in evaluating movement requirements of different training activities and pitch dimensions. The findings suggest such a variable offers value in a monitoring strategy in football.
Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein–coupled PLC signalling in vivo

In Drosophila photoreceptors, PI(4,5)P2 synthesis during PLC signalling is regulated by PIP5K and septins. Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] hydrolysis by phospholipase C (PLC) is a conserved mechanism of signalling. Given the low abundance of PI(4,5)P2, its hydrolysis needs to be coupled to resynthesis to ensure continued PLC activity; however, the mechanism by which depletion is coupled to resynthesis remains unknown. PI(4,5)P2 synthesis is catalyzed by the phosphorylation of phosphatidylinositol 4 phosphate (PI4P) by phosphatidylinositol 4 phosphate 5 kinase (PIP5K). In Drosophila photoreceptors, photon absorption is transduced into PLC activity and during this process, PI(4,5)P2 is resynthesized by a PIP5K. However, the mechanism by which PIP5K activity is coupled to PI(4,5)P2 hydrolysis is unknown. In this study, we identify a unique isoform dPIP5KL, that is both necessary and sufficient to mediate PI(4,5)P2 synthesis during phototransduction. Depletion of PNUT, a non-redundant subunit of the septin family, enhances dPIP5KL activity in vitro and PI(4,5)P2 resynthesis in vivo; co-depletion of dPIP5KL reverses the enhanced rate of PI(4,5)P2 resynthesis in vivo. Thus, our work defines a septin-mediated mechanism through which PIP5K activity is coupled to PLC-mediated PI(4,5)P2 hydrolysis.
Application of the Simple Linear Regression Model in the Experiment

The paper presents the results of experiments and measurements of the stress of a pipeline located on a pipe bridge. The experiment was carried out by means of resistance strain gages for two modes, which were the stress changes upon pressure changes in the pipes and at the driving in of sheet pilings in the immediate vicinity of the pipeline. On the basis of measurements, the impact of these changes on the operational safety of the pipeline in the renovation process of old above ground and the realization of new underground pipeline solutions. Polynomial regression models were applied for the prediction and a statistical analysis of residuals was applied for the quality assessment of selected models.
A Study of Network 2-Factor Access Control Model for Prevention the Medical-Data Leakage

Abstract Network Access Control system of medical asset protection solutions that installation and operation on system and network to provide a process that to access internal network after verifying the safety of information communication devices. However, there are still the internal medical-data leakage threats due to spoof of authorized devices and unauthorized using of users are away hours. In this paper, Network 2-Factor Access Control Model proposed for prevention the medical-data leakage by improving the current Network Access Control system. The proposed Network 2-Factor Access Control Model allowed to access the internal network only actual users located in specific place within the organization and used authorized devices. Therefore, the proposed model to provide a safety medical asset environment that protecting medical-data by blocking unauthorized access to the internal network and unnecessary internet access of authorized users and devices. Key Words : Access Control, Information Security, Identification, NAC, Medical Asset Protection
The onset of tapering in the early stage of growth of a nanowire

The early stage of growth of semiconductor nanowires is studied in the case where the sidewall adatoms have a short diffusion length due to a strong desorption. Experimental results are described for the growth of ZnSe nanowires by molecular beam epitaxy. They are discussed and interpreted using the Burton–Cabrera–Frank description of the propagation of steps along the sidewalls, and compared to other II–VI and III–V nanowires. The role of the growth parameters and the resulting shape of the nanowires (cylinder, cone, or both combined) are highlighted.
The impact of anti‐COVID‐19 nonpharmaceutical interventions on hand, foot, and mouth disease—A spatiotemporal perspective in Xi'an, northwestern China

Growing evidence has shown that anti‐COVID‐19 nonpharmaceutical interventions (NPIs) can support prevention and control of various infectious diseases, including intestinal diseases. However, most studies focused on the short‐term mitigating impact and neglected the dynamic impact over time. This study is aimed to investigate the dynamic impact of anti‐COVID‐19 NPIs on hand, foot, and mouth disease (HFMD) over time in Xi'an City, northwestern China. Based on the surveillance data of HFMD, meteorological and web search data, Bayesian Structural Time Series model and interrupted time series analysis were performed to quantitatively measure the impact of NPIs in sequent phases with different intensities and to predict the counterfactual number of HFMD cases. From 2013 to 2021, a total number of 172,898 HFMD cases were reported in Xi'an. In 2020, there appeared a significant decrease in HFMD incidence (−94.52%, 95% CI: −97.54% to −81.95%) in the first half of the year and the peak period shifted from June to October by a small margin of 6.74% compared to the previous years of 2013 to 2019. In 2021, the seasonality of HFMD incidence gradually returned to the bimodal temporal variation pattern with a significant average decline of 61.09%. In particular, the impact of NPIs on HFMD was more evident among young children (0–3 years), and the HFMD incidence reported in industrial areas had an unexpected increase of 51.71% in 2020 autumn and winter. Results suggested that both direct and indirect NPIs should be implemented as effective public health measures to reduce infectious disease and improve surveillance strategies, and HFMD incidence in Xi'an experienced a significant rebound to the previous seasonality after a prominent decline influenced by the anti‐COVID‐19 NPIs.