Miracle-dz/UniFAR-FTUs huggingface dataset:
Complex Movement Control in a Rat Model of Parkinsonian Falls: Bidirectional Control by Striatal Cholinergic Interneurons

Older persons and, more severely, persons with Parkinson's disease (PD) exhibit gait dysfunction, postural instability and a propensity for falls. These dopamine (DA) replacement-resistant symptoms are associated with losses of basal forebrain and striatal cholinergic neurons, suggesting that falls reflect disruption of the corticostriatal transfer of movement-related cues and their striatal integration with movement sequencing. To advance a rodent model of the complex movement deficits of Parkinsonian fallers, here we first demonstrated that male and female rats with dual cortical cholinergic and striatal DA losses (DL rats) exhibit cued turning deficits, modeling the turning deficits seen in these patients. As striatal cholinergic interneurons (ChIs) are positioned to integrate movement cues with gait, and as ChI loss has been associated with falls in PD, we next used this task, as well as a previously established task used to reveal heightened fall rates in DL rats, to broadly test the role of ChIs. Chemogenetic inhibition of ChIs in otherwise intact male and female rats caused cued turning deficits and elevated fall rates. Spontaneous turning was unaffected. Furthermore, chemogenetic stimulation of ChIs in DL rats reduced fall rates and restored cued turning performance. Stimulation of ChIs was relatively more effective in rats with viral transfection spaces situated lateral to the DA depletion areas in the dorsomedial striatum. These results indicate that striatal ChIs are essential for the control of complex movements, and they suggest a therapeutic potential of stimulation of ChIs to restore gait and balance, and to prevent falls in PD. SIGNIFICANCE STATEMENT In persons with Parkinson's disease, gait dysfunction and the associated risk for falls do not benefit from dopamine replacement therapy and often result in long-term hospitalization and nursing home placement. Here, we first validated a new task to demonstrate impairments in cued turning behavior in rodents modeling the cholinergic-dopaminergic losses observed in Parkinsonian fallers. We then demonstrated the essential role of striatal cholinergic interneurons for turning behavior as well as for traversing dynamic surfaces and avoiding falls. Stimulation of these interneurons in the rat model rescued turning performance and reduced fall rates. Our findings indicate the feasibility of investigating the neuronal circuitry underling complex movement control in rodents, and that striatal cholinergic interneurons are an essential node of such circuitry.
Invertebrates in Myriophyllum spicatum L. stands in lake Sakadaš, Croatia.

The invertebrates associated with Myriophyllum spicatum L. , were studied during the vegetation period in 2004, at three stations I, II and III, in lake Sakadaš within Kopački rit floodplain, Croatia. A total of twenty one taxa were recorded, with the dominance of the insect larvae representatives. Family Chironomidae had the greatest abundance and represented 67% of all recorded invertebrates, while as nematodes and oligochaetes were especially abundant at the third station. Significant differences in invertebrate abundances were recorded between months of sampling – July and August as well as July and September. Also, stations I and II showed statisticaly important differences in relation to station III, located near lake’s dam. Physicochemical data, including trophic state indices, as well as invertebrate community composition were indicative for eutrophic state of water with a tendency to hypertrophy at all stations in lake Sakadaš.
FlexEval, création de sites web légers pour des campagnes de tests perceptifs multimédias (FlexEval, creation of light websites for multimedia perceptual test campaigns)

Nous présentons FlexEval, un outil de conception et déploiement de tests perceptifs multimédias sous la forme d’un site web léger. S’appuyant sur des technologies standards et ouvertes du web, notamment le framework Flask, FlexEval offre une grande souplesse de conception, des gages de pérennité, ainsi que le support de communautés actives d’utilisateurs. L’application est disponible en open-source via le dépôt Git https://gitlab.inria. fr/expression/tools/flexeval.
Secular Trends and Geographic Maps of Hepatitis C Virus Infection among 4 Million Blood Donors in Taiwan from 1999 to 2017

The prevalence of hepatitis C virus (HCV) infection in Taiwan was approximately 4% a decade ago, much higher than the worldwide average. This study aimed to assess the HCV burden among 4 million voluntary blood donors after 2 decades of prevention and treatment policies. We retrieved screening results for anti‐HCV and HCV RNA from the Database for Evaluating Voluntary Taiwanese Eligible Donors. First‐time blood donors who donated blood after 1999 and repeat donors who donated blood more than once between 2013 and 2017 were included to estimate HCV prevalence and incidence, respectively. The Cox proportional hazards model was used to estimate hazard ratios. Geographic variation in HCV prevalence and incidence in 364 townships was also analyzed. The prevalence study included 3,656,598 first‐time donors. The overall crude prevalence of anti‐HCV decreased from 15.5 to 4.5 per 1,000 donors between 1999 and 2017. Younger birth cohorts had a significantly lower prevalence of anti‐HCV. The majority of townships (64.3%) in Taiwan showed a significantly decreased prevalence. The incidence study included 1,393,014 repeat donors followed for 3,436,607 person‐years. Ninety‐eight donors seroconverted to HCV RNA positivity, resulting in an HCV incidence of 2.9 per 100,000 person‐years. Donors living in townships where HCV RNA prevalence was greater than 2 per 1,000 had at least 2.5‐fold greater risk of new HCV infection. Conclusion: HCV prevalence in Taiwanese first‐time blood donors decreased by 71% in the last 2 decades. However, townships with higher HCV prevalence also showed higher HCV incidence and require more active intervention.
Anti-apoptotic signaling by hepatocyte growth factor/Met via the phosphatidylinositol 3-kinase/Akt and mitogen-activated protein kinase pathways.

Hepatocyte growth factor (HGF) is a ligand of the receptor tyrosine kinase encoded by the c-Met protooncogene. HGF/Met signaling has multifunctional effects on various cell types. We sought to determine the role of HGF/Met in apoptosis and identify signal transducers involved in this process. In experiments with human SK-LMS-1 leiomyosarcoma cells, we show that the Akt kinase is activated by HGF in a time- and dose-dependent manner by phosphatidylinositol 3-kinase (PI3-kinase). Akt is also activated by active tumorigenic forms of Met, i.e., ligand-independent Tpr-Met, a truncated and constitutively dimerized form of Met, and a mutationally activated version of Met corresponding to that found in human hereditary papillary renal carcinoma. In NIH 3T3 cells transfected with wild-type Met, HGF inhibits apoptosis induced by serum starvation and UV irradiation. HGF-induced survival correlates with Akt activity and is inhibited by the specific PI3-kinase inhibitor LY294002, indicating that HGF inhibits cell death through the PI3-kinase/Akt signal transduction pathway. Furthermore, transiently transfected Tpr-Met activates Akt (both Akt1 and Akt2) and protects cells from apoptosis. Mitogen-activated protein kinase (MAPK) also is activated by HGF and rescues cells from apoptosis, although the cytoprotective effect is less marked than for PI3-kinase/Akt. Blocking MAPK with the specific MAPK kinase inhibitor PD098059 impairs the ability of HGF to promote cell survival. Similar results were obtained with NIH 3T3 cells expressing the fusion protein Trk-Met and stimulated with nerve growth factor, the Trk ligand. These results demonstrate that HGF/Met is capable of protecting cells from apoptosis by using both PI3-kinase/Akt and, to a lesser extent, MAPK pathways.
Development of a droplet digital PCR for detection and quantification of porcine epidemic diarrhea virus

Porcine epidemic diarrhea, a disease caused by porcine epidemic diarrhea virus (PEDV), results in large economic losses to the global swine industry. To manage this disease effectively, it is essential to detect PEDV early and accurately. We developed a sensitive and accurate droplet digital PCR (ddPCR) assay to detect PEDV. The optimal primer-to-probe concentration and melting temperature were identified as 300:200 nM and 59.2°C, respectively. The specificity of the ddPCR assay was confirmed by negative test results for common swine pathogens. The detection limit for the ddPCR was 0.26 copies/μL, which is a 5.7-fold increase in sensitivity compared to that of real-time PCR (rtPCR). Both ddPCR and rtPCR assays exhibited good linearity, although ddPCR provided higher sensitivity for clinical detection compared to that of rtPCR. Our ddPCR methodology provides a promising tool for evaluating the PEDV viral load when used for clinical testing, particularly for detecting samples with low-copy viral loads.
Characteristics of microseismic data recorded by distributed acoustic sensing systems in anisotropic media

Fiber-optic distributed acoustic sensing (DAS) cables are now used to monitor microseismicity during hydraulic-fracture stimulations of unconventional gas reservoirs. Unlike geophone arrays, DAS systems are sensitive to uniaxial strain or strain rate along the fiber direction and thus provide a 1C recording, which makes identifying the directionality and polarization of incoming waves difficult. Using synthetic examples, we have shown some fundamental characteristics of microseismic recordings on DAS systems for purposes of hydraulic fracture monitoring in a horizontal well in anisotropic (vertical transverse isotropy [VTI]) shales. We determine that SH arrivals dominate the recorded signals because their polarization is aligned along the horizontal cable at the near offset, although SV will typically dominate for events directly above or below the array. The amplitude of coherent shear-wave (S-wave) arrivals along the cable exhibits a characteristic pattern with bimodal peaks, the width of which relates to the distance of the event from the cable. Furthermore, we find that S-wave splitting recorded on DAS systems can be used to infer the inclination of the incoming waves, overcoming a current limitation of event locations that have constrained events to lie in a horizontal plane. Low-amplitude SV arrivals suggest an event depth similar to that of the DAS cable. Conversely, steep arrivals produce higher amplitude SV-waves, with S-wave splitting increasing with offset along the cable. Finally, we determine how polarity reversals observed in the P and SH phases can be used to provide strong constraints on the source mechanisms.
Decay of Apparent Individual Distinctiveness in the Begging Calls of Adult Female White-Throated Magpie-Jays

Abstract Individual recognition, the ability to correctly identify an individual, relies upon individual distinctiveness in a trait being used to determine identity. However, if such distinctiveness is not stable over time, its usefulness is limited. The purpose of this study was to assess individual distinctiveness in the begging calls of female White-throated Magpie-Jays (Calocitta formosa) recorded over different time periods. Such food solicitation calls could be selected to encode caller identity if multiple individuals within a group or neighborhood produce them. On average, a discriminant function analysis of 29 call parameters correctly assigned 51% of each female's calls to the appropriate female, suggesting that begging calls are individually distinctive. However, females that were recorded on many days were more likely to have their calls incorrectly assigned than were females recorded on one or a few days. On a short time scale, females produce calls consistently, but calls vary over longer time scales, causing a significant decrease in assignment accuracy. Such decay in accuracy raises questions about reported individual distinctiveness in other species that have not been sampled over longer time periods or on multiple occasions. In White-throated Magpie-Jays, floater males looking for fertile females, or group members determining whom to feed could benefit from individuality in begging, but they may need to integrate identity information over multiple calls in order to correctly determine sender identity.
Effect of carbohydrase supplementation of wheat- and canola-meal-based diets on growth performance and nutrient digestibility in group-housed weaned pigs

Fibrous components in canola meal and wheat limit their effective use in diets for weaned pigs. Effects of supplementing a carbohydrase (845 and 327 Units g-1 xylanase and β-glucanase) to a wheat-canola meal diet on growth performance, small intestine nutrient digestibility and digesta viscosity were investigated. Ninety-six weaned pigs (7.2 ± 1.2 kg) had free access to control diet [65% wheat, 25% canola meal; 3.15 Mcal digestible energy (DE) kg-1, 10.6 g digestible lysine kg-1] or control diet supplemented with carbohydrase at three inclusion rates (1, 2, or 4 g kg-1) for 28 d. Four pigs were housed per pen for six pens per diet. On days 19 to 21, six pigs per diet were euthanised to collect small intestine digesta. Carbohydrase increased average daily feed intake (ADFI) and average daily gain (ADG) quadratically (P 0.10). The ADG was p.
Three new lichen species from Macaronesia belonging in Ramalinaceae, with the description of a new genus

Tylocliostomum is described as a new genus and T. viridifarinosum as a new species. Two Bacidina species, B. pallidocarpa and B. violacea, are also described as new to science. They all occur in Macaronesia (Azores, Madeira).
Shoot position affects root initiation and growth of dormant unrooted cuttings of Populus

Rooting of dormant unrooted cuttings is crucial to the commercial deployment of intensively cultured poplar (Populus spp.) plantations because it is the first biological prerequisite to stand establishment. Rooting can be genetically controlled and subject to selection. Thus, our objective was to test for differences in rooting ability among cuttings from three positions on cutting orchard plants of five genomic groups ([Bartr. ex Marsh x P. trichocarpa Torr. & Gray P. deltoides] x P. deltoides 'BC', P. deltoides 'D;, P. deltoides H P. maximowiczii A. Henry 'DM', P. deltoides x P. nigra L. 'DN', P. nigra x P. maximowiczii 'NM').
Single DC Source Multilevel Inverter with Changeable Gains and Levels for Low-Power Loads

Different types of multilevel converters have been used to convert DC voltage to AC voltage for different applications. It is, however, desirable for flexible AC output voltage to be created from a single DC source at lower cost. This paper presents a new type of single DC source multilevel inverter which has the ability to create a different number of levels with low rate components. This device is also able boost the magnitude of the output voltage by means of a variable gain with one DC source. The advantages of the multilevel inverter are rapid stepping among levels, and its ability to produce different ranges of levels (seven, nine and eleven) and gains (two, four and eight). The proposed multilevel inverter includes six semiconductor switches, eight diodes, two capacitors and two inductors, making it suitable for low-power applications. A simulation in MATLAB and experimental tests on a prototype setup showed good performance for different modulations, with THD% down to 2.29%, which are meets the IEEE standard (IEEE 519).
Generative Adversarial Networks (GANs) Based Synthetic Sampling for Predictive Modeling

In the present report we evaluate the possible utility of the Generative Adversarial Networks (GANs) in mapping the chemical structural space for molecular property profiles, with the goal of subsequently yielding synthetic (artificial) samples for ligand‐based molecular modeling. Two case studies are considered: BACE‐1 (β‐Secretase 1) and DENV (Dengue Virus) inhibitory activities, with the former focused on data populating and the latter on data balancing tasks. We train GANs using subsamples extracted from datasets for each bioactivity endpoint, and apply the trained networks in generating synthetic examples from the respective bioactivity chemical spaces. Original and synthetic samples are pooled together and employed to build BACE‐1 and DENV inhibitory activity classifiers and their performance evaluated over tenfold external validation sets. In both case studies, the obtained classifiers demonstrate satisfactory predictivity with the former yielding accuracy (ACC) and Mathew's correlation coefficient (MCC) values of 0.80 and 0.59, while the latter produces balanced accuracy(BACC) and MCC values of 0.81 and 0.70, respectively. Moreover, the statistics of these classifiers are compared with those of other models in the literature demonstrating comparable to better performance. These results suggest that GANs may be useful in mapping the chemical space for molecular property profiles of interest, and thus allow for the extraction of synthetic examples for computational modeling.
Boron tolerance in wheat in relation to soil salinity

SUMMARY A screenhouse study in Haryana in 1986/87 evaluated the effect of boron on the wheat cultivar WH147 grown in pots in a sandy soil at three salinities. Before sowing, 0–9 mg B/kg soil was added. Without added B the crop produced significantly more grain at electrical conductivity of the saturation extract (ECe) 6 dS/m than at other salinities, but at ECe 8 dS/m yields were similar to those in nonsaline (ECe 0·8) soil. Grain yield in nonsaline soil was not affected significantly even at the highest concentration of added B, even though at the boot stage the shoots had accumulated as much as 307 mg B/kg. At ECe 6 and 8 dS/m, grain yield was significantly less with 3 and 1 mg added B/kg, respectively, than without added B, even though the B concentration in the shoots was only 72 and 31 mg/kg, respectively. The results indicated that B tolerance decreased with increasing salinity of the soil, even though B absorption by plants was 24–62% less in saline than in nonsaline soil at similar B concentrations. As B and salinity often occur together, threshold values of B in soil and plants for optimum growth of wheat will differ with the ECe of the soil. The results suggested that Ca:B ratio in wheat straw is not a reliable indicator of B toxicity in the soil.
Evaluation of Media for Counting Viable Bacteria in Estuaries

1 Plate counts of viable bacteria in estuarine water samples were made using seven standard agar media. 2 Highest counts were obtained on Anderson's medium which is therefore recommended for general estuarine use. 3 The composition of Anderson's medium was peptone 2.5 g, yeast extract 2.5 g, FePO4 0.1 g and agar 15.0 g, made up to 11 with artificial estuary water.
Computer Programs

Music Publisher, and Presto Keyboard, Version 2.0 and updates, registered trademarks of Repertoire Pty Ltd, 49A Stephens Terrace, St Peters. S.A. 5069, Australia, 1988. Music Publisher is designed to combine the type-setting of conventional music and text in the fastest and most efficient way possible, and to deliver the highest quality reproduction available by laser printing. The professional software operates using the accompanying Presto entry keypad and is compatible with the Macintosh computer (ie Mac Plus, SE and Macintosh II). The recommended minimum memory requirement is one megabyte. The package comes with a 204 page manual that includes a 27 page (five lesson) tutorial. The tutorial section in particular is clearly laid out and introduces the reader to the main features of this sophisticated program. After setting up the accompanying Presto entry keypad I was able to use almost all features of Music
Design of a wideband right‐handed circularly polarized array of miniaturized mushroom‐shaped antennas for direct broadcast satellite application

In this study, a wideband right‐handed circularly polarized (RHCP) array of microstrip patch antennas, for direct broadcast satellite (DBS, 12.2‐12.7 GHz) services have been presented. The single element of the array is a two‐dimensional mushroom‐shaped fractal antenna of resonating frequency 12.49 GHz and an impedance bandwidth of 1.12 GHz. The proposed single element is designed by etching a pair of Minkowski square boxes in one of the two opposite sides of a regular octagon. Furthermore, a thin slot of length equal to one side of the regular octagon is placed in‐between the two Minkowski boxes, which results in the proposed mushroom‐shaped radiating element. The proposed four‐element linear array resonates at 12.47 GHz and gives an impedance bandwidth of 1.04 GHz. The feed network of the array is designed in such a manner to make the array structure very compact. The fabricated single element gives an RHCP gain of 5.25 dBic and its corresponding circular Polarization (CP) bandwidth of 200 MHz. The array gives an RHCP gain of 11.59 dBic and a CP bandwidth of 460 MHz. The measured CP bandwidth of the fabricated array prototype ensures 92% coverage of the entire DBS band with circularly polarized wave, and hence can be used for its related applications.
Characterization of diversity and genetic structure in natural populations of Stryphnodendron adstringens (Mart.) Coville by means of allozyme markers

The S. adstringens, a typical Cerrado (Brazilian savannah) tree, is used because of its medicinal and tanning properties. Because of the still incipient genetic knowledge of the species, the objective of this work was to characterize the diversity and genetic structure of S. adstringens by using allozyme markers. Seeds were collected in five Brazilian mesoregions, in which 627 individuals in 16 populations in the states of Minas Gerais and Goias were sampled. Fourteen isoenzyme systems were assessed, out of which seven were polymorphic with a total of 10 loci and 28 alleles. Average genetic diversity (H) was 0.226, average proportion of polymorphic loci (P) was 68.75, average number of alleles per polymorphic locus (AP) was 2.65 and effective number of alleles (Ae) was equal to 1.29. The results of total fixation index (F= 0.003), within population fixation index (f =-0.114) and genetic differentiation measure (θ =0.105) were not significant, which shows the inexistence of genetic structure. Two principal groups were found in the cluster analysis (UPGMA), where the first one was formed by the population of State Park (PE) of Rio Preto (MG) and the other, by the other populations. If the population of PE of Rio Preto is excluded from the analysis, GST is drastically reduced from 0.077 to 0.026. Thus, approximately 2/3 of the total value of GST found in S. adstringens was due to the variation among the population of PE of Rio Preto and the other populations. Overall, the values of H and P found in S. adstringens are compatible with the ones found in typically distributed tropical trees. On the other hand, by excluding the population of PE of Rio Preto, the value of the GST genetic differentiation measure was smaller than the one found in native tropical trees from temperate zones. The similarity between the assessed populations shows that the gene flow is still high enough to avoid genetic differentiation, at the local level, at least.