Miracle-dz/UniFAR-FTUs huggingface dataset:
S1P2, the G protein-coupled receptor for sphingosine-1-phosphate, negatively regulates tumor angiogenesis and tumor growth in

Sphingosine-1-phosphate (S1P) has been implicated in tumor angiogenesis by acting via a Gi-coupled chemotactic receptor S1P1. We report here that S1P2, which, distinctly from S1P1, mediates S1P inhibition of Rac and cell migration via G12/13 and the Rho pathway, negatively regulates tumor angiogenesis and tumor growth. By using S1P2 LacZ/+ mice, we found that S1P2 was expressed in tumor vessels and normal blood vessels in many organs in both endothelial cells (ECs) and vascular smooth muscle cells, as well as tumor-associated, CD11b-positive bone marrow-derived cells (BMDCs). Lewis lung carcinoma (LLC) and B16BL6 melanoma tumors implanted in S1P2-deficient (S1P2) mice showed accelerated tumor growth and stimulated angiogenesis with enhanced association of vascular smooth muscle cells and pericytes. Compared with S1P2 ECs, S1P2 ECs showed enhanced Rac activity, Akt phosphorylation, cell migration, proliferation and tube formation in vitro. Co-injection of S1P2 ECs and tumor cells into S1P2 mice enhanced tumor growth and angiogenesis in vivo, as compared with co-injection of S1P2 ECs and tumor cells. The recruitment of CD11b-positive BMDCs into tumors in S1P2 mice was increased, compared with tumors in S1P2 siblings. The bone marrow transplantation experiments showed that deletion of S1P2 exclusively in BMDCs promoted tumor growth and angiogenesis. These results indicate that, in contrast to endothelial S1P1 which stimulates tumor angiogenesis, S1P2 on ECs and BMDCs mediates potent inhibition of tumor angiogenesis, providing a novel therapeutic strategy for anti-cancer treatment.
Cytokine Pro le During Occult Hepatitis B Virus Infection in Chronic Hepatitis C Patients

Camilla Rodrigues de Almeida Ribeiro Fundacao Oswaldo Cruz Nathalia Alves Araújo de Almeida Fundacao Oswaldo Cruz Katrini Guidolini Martinelli Universidade Federal do Espirito Santo Marcia Amendola Pires Gaffrée and Guinle Universitary Hospital Carlos Eduardo Brandao Mello Gaffrée and Guinle Universitary Hospital José J. Barros (  barros@ioc. ocruz.br ) Fundacao Oswaldo Cruz Vanessa Salete De Paula Fundacao Oswaldo Cruz
Supplementary Information for “Decomposing information into copying versus transformation”

D x satisfies Axiom 1 by non-negativity of KL. It satisfies Axiom 2 when pY |x(x) > pY (x) because d(pY |x(x), pY (x)) ≤ DKL(pY |x‖pY ) by the data processing inequality for KL divergence [1, Lemma 3.11]. Otherwise, when pY |x(x) ≤ pY (x), D x vanishes and thus satisfies Axiom 2 trivially. It satisfies Axiom 3 when pY |x(x) ≤ pY (x) because in that case D x (pY |x‖pY ) = 0 ≤ D x (pY |x(x)‖pY ). If pY |x(x) ≤ pY (x), then note that the derivative of d(a, b) with respect to a is d dad(a, b) = log a b − log 1−a 1−b , which is strictly positive when a > b. Finally, we show that D x satisfies Axiom 4. For any prior distribution pY , define the following posterior distribution pY |x(y):
Partnership in Ig-Secreting Cells Critical Role for the Oct-2/OCA-B

B and T lymphocytes arise from a common precursor in the bone marrow, but ultimately acquire very different functions. The difference in function is largely attributable to the expression of tissue-speciﬁc transcription factors that activate discrete sets of genes. In previous studies we and others have shown that the specialized genes expressed by Ig-secreting cells cease transcription when these cells are fused to a T lymphoma. The extinguished genes include those encoding Ig, J chain, and the transcription factors Oct-2, PU.1, and the coactivator OCA-B. Remarkably, if we sustain Oct-2 expression during cell fusion, all the other tissue-speciﬁc genes of the Ig-secreting cell simultaneously escape silencing. This suggests that Oct-2 plays a central role in maintaining the gene expression program of these cells. In the present studies we have investigated the roles of the transcription factor PU.1 and the coactivator OCA-B within the hierarchy of regulatory factors that sustain Ig-secreting cell function. Our results show that OCA-B and Oct-2 are regulatory partners in this process and that PU.1 plays a subordinate role at this cell stage. The Journal of Immunology, 2003, 171: 6589–6598.
Bayesian Analysis of Linear Inverse Problems with Applications in Economics and Finance

In my PhD thesis I propose a Bayesian nonparametric estimation method for structural econometric models where the functional parameter of interest describes the economic agent's behavior. The structural parameter is characterized as the solution of a functional equation, or by using more technical words, as the solution of an inverse problem that can be either ill-posed or well-posed. From a Bayesian point of view, the parameter of interest is a random function and the solution to the inference problem is the posterior distribution of this parameter. A regular version of the posterior distribution in functional spaces is characterized. However, the infinite dimension of the considered spaces causes a problem of non continuity of the solution and then a problem of inconsistency, from a frequentist point of view, of the posterior distribution (i.e. problem of ill-posedness). The contribution of this essay is to propose new methods to deal with this problem of ill-posedness. The first one consists in adopting a Tikhonov regularization scheme in the construction of the posterior distribution so that I end up with a new object that I call regularized posterior distribution and that I guess it is solution of the inverse problem. The second approach consists in specifying a prior distribution on the parameter of interest of the g-prior type. Then, I detect a class of models for which the prior distribution is able to correct for the ill-posedness also in infinite dimensional problems. I study asymptotic properties of these proposed solutions and I prove that, under some regularity condition satisfied by the true value of the parameter of interest, they are consistent in a "frequentist" sense. Once I have set the general theory, I apply my bayesian nonparametric methodology to different estimation problems. First, I apply this estimator to deconvolution and to hazard rate, density and regression estimation. Then, I consider the estimation of an Instrumental Regression that is useful in micro-econometrics when we have to deal with problems of endogeneity. Finally, I develop an application in finance: I get the bayesian estimator for the equilibrium asset pricing functional by using the Euler equation defined in the Lucas'(1978) tree-type models.
Airborne Broadband Ultrasound: Wireless Channel and Motion Tracking Algorithms

—Airborne broadband ultrasound (ABU) covers a wide stretch of inaudible acoustic frequencies which have been used to provide scalable (i.e. multiuser) and highly robust ranging and localization of static tags in indoor environments. This is useful for mobile computing applications and robotics. However, because ABU signals are wideband and propagate relatively slowly, they are prone to aggressive Doppler distortion as a result of human-scale movements indoors. Additionally, the ABU channel is characterized by pronounced distance-dependent variations. This paper gives a treatment of the ABU channel both theoretically and experimentally, in order to inform the design of ABU tracking systems. Then, Doppler-tolerant ABU tracking algorithms are developed. Their performance is reported, using a direct-sequence (DS) code-division multiple access (CDMA) signalling approach. Successful motion inference is demonstrated in the ABU band.
The Progression Related Gene RAB42 Affects the Prognosis of Glioblastoma Patients

Background: Glioblastoma (GBM) represents the most malignant glioma among astrocytomas and is a lethal form of brain cancer. Many RAB genes are involved in different cancers but RAB42 (Ras-associated binding 42) is seldom studied in GBM. Our study aimed to explore the role of RAB42 expression in the development and prognosis of GBM. Methods: All GBM patient data were obtained from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. The relevance of RAB42 expression to the clinicopathologic characteristics of GBM patients was analyzed. The overall survival (OS) significance was determined using log-rank. Significantly enriched KEGG pathways were screened using gene set enrichment analysis (GSEA). Results: High expression of RAB42 was observed in GBM specimens compared with normal samples, which was also verified in cell lines and tissue samples. Elevated RAB42 expression was correlated with higher GBM histological grade. The prognosis of GBM patients with high RAB42 expression was worse than those with lower RAB42. A total of 35 pathways, such as the P53 pathway, were significantly activated in highly RAB42-expressed GBM samples. Conclusions: High RAB42 expression is related to the development of GBM, and RAB42 is a probable prognostic marker for GBM.
Origanum majorana L. Extract Attenuated Benign Prostatic Hyperplasia in Rat Model: Effect on Oxidative Stress, Apoptosis, and Proliferation

Benign prostatic hyperplasia (BPH) is a widespread androgenic illness influencing elderly men. It is distinguished by prostatic epithelial and stromal muscle cell proliferation. Inflammation, oxidative stress, and apoptosis have all been interrelated to the development of BPH. Marjoram (Origanum majorana L.) is a herb with reported antiproliferative, proapoptotic, and antioxidative properties, which have not yet been studied in relation to BPH. Consequently, in this work, an ethanolic extract of O. majorana was prepared in two doses (250 and 500 mg/kg/day) to be injected into castrated rats after induction of a testosterone-BPH model. Testosterone propionate (TP) was subcutaneously injected (0.5 mg/kg/day) for one week after castration to induce BPH. Forty adult Wistar male rats were randomly allocated into five groups: control, BPH model, high and low O. majorana doses (250, 500 mg/kg/day), and finasteride (FN) (0.8 mg/kg/day) as a positive control. Treatment was continued with drugs/normal saline for 28 days. Rat’s body and prostate were weighed, prostate index (PI) and % of prostate growth inhibition were calculated, serum dihydrotestosterone (DHT), prostatic content of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), and malondialdehyde (MDA), DN damage, histopathological changes, immune expression of proliferating cell nuclear antigen (PCNA), caspase-3, α-SMA, and TGF-β1 were assessed. In addition, molecular quantitative PCR and ELISA analyses were performed to identify the expression of mRNAs and related proteins of both caspase-3 and TGF-β1 in prostate tissue from O. majorana-treated and untreated groups. Rats with BPH had significantly higher prostate weights and PI, higher DHT, DNA damage (8-hydroxyguanine, 8-OH-dG), and MDA levels with prominent PCNA, α-SMA, and TGF-β expression, but lower SOD, CAT, and TAC activity and caspase-3 expression. O. majorana (250 and 500 mg/kg/day)-treated groups revealed a decrease in prostate weights and PI, lower levels of DHT, suppressed oxidative stress, reduced tissue proliferation and fibrosis, and restored antioxidant and proapoptotic activity. Additionally, quantitative PCR and ELISA analysis showed that treatment with O. majorana significantly upregulated the expression of caspase-3 and downregulated the expression of TGF-β in prostate tissues of BPH rats. The data were confirmed by the immunohistological reactivity of these targeted markers in the prostate tissues. These effects were more significant with O. majorana 500 mg/mL/rat. In conclusion, the current study indicates the efficient use of O. majorana in the treatment of testosterone-induced BPH through its antiproliferative, proapoptotic, and antioxidative mechanisms.
The Effects of 1 Egg per Day on Iron and Anemia Status among Young Malawian Children: A Secondary Analysis of a Randomized Controlled Trial

ABSTRACT Background Young children with diets lacking diversity with low consumption of animal source foods are at risk of iron deficiency anemia (IDA). Objectives Our objectives were to determine the impact of supplementing diets with 1 egg/d on 1) plasma ferritin, soluble transferrin receptor (sTfR), body iron index (BII), and hemoglobin concentrations and 2) the prevalence of iron deficiency (ID), anemia, and IDA. Methods Malawian 6–9-mo-old infants in the Mazira trial (clinicaltrials.gov; NCT03385252) were individually randomly assigned to receive 1 egg/d for 6 mo (n = 331) or continue their usual diet (n = 329). In this secondary analysis, hemoglobin, plasma ferritin, sTfR, C-reactive protein (CRP), and α-1-acid glycoprotein (AGP) were measured at enrollment and 6-mo follow-up. Iron biomarkers were corrected for inflammation. Ferritin, sTfR, BII, and hemoglobin were compared between groups using linear regression. Prevalence ratios (PRs) for anemia (hemoglobin <11 g/dL) and ID (ferritin <12 µg/L, sTfR >8.3 mg/L, or BII <0 mg/kg) between groups were compared using log binomial or modified Poisson regression. Results A total of 585 children were included in this analysis (Egg: n = 286; Control: n = 299). At enrollment, the total prevalence of anemia was 61% and did not differ between groups. At 6-mo follow-up, groups did not differ in geometric mean concentration of hemoglobin [mean (95% CI); Egg: 10.9 (10.7, 11.1) g/dL; Control: 11.1 (10.9, 11.2) g/dL] and inflammation-adjusted ferritin [Egg: 6.52 (5.98, 7.10) µg/L; Control: 6.82 (6.27, 7.42) µg/L], sTfR [Egg: 11.34 (10.92, 11.78) mg/L; Control: 11.46 (11.04, 11.89) mg/L] or BII [Egg: 0.07 (0.06, 0.09) mg/kg; Control: 0.07 (0.05, 0.08) mg/kg]. There were also no group differences in anemia [Egg: 46%; Control 40%; PR: 1.15 (95% CI: 0.96, 1.38)], ID [PR: 0.99 (0.94, 1.05)], or IDA [PR: 1.12 (0.92, 1.36)]. Conclusions Providing eggs daily for 6 mo did not affect iron status or anemia prevalence in this context. Other interventions are needed to address the high prevalence of ID and anemia among young Malawian children. This trial is registered at http://www.clinicaltrials.gov as NCT03385252.
Vascularization of Cell-Laden Microfibres by Femtosecond Laser Processing

To face the increasing demand for organ transplantation, currently the development of tissue engineering appears as the best opportunity to effectively regenerate functional tissues and organs. However, these approaches still face the lack of an efficient method to produce an efficient vascularization system. To answer these issues, the formation of an intra-volume channel within a three-dimensional, scaffold free, mature, and cell-covered collagen microfibre is here investigated through laser-induced cavitation. An intra-volume channel was formed upon irradiation with a near-infrared, femtosecond laser beam, focused with a high numerical aperture lens. The laser beam directly crossed the surface of a dense and living-cell bilayer and was focused behind the bilayer to induce channel formation in the hydrogel core while preserving the cell bilayer. Channel formation was assessed through confocal microscopy. Channel generation inside the hydrogel core was enhanced by the formation of voluminous cavitation bubbles with a lifetime longer than 30 s, which also improved intra-volume channel durability. Twenty-four hours after laser processing, cellular viability dropped due to a lack of sufficient hydration for processing longer than 10 min. However, the processing automation could drastically reduce the cellular mortality, this way enabling the formation of hollowed microfibres with a high density of living-cell outer bilayer.
E¡ect of host Lewis and ABO blood group antigen expression on Helicobacter pylori colonisation density and the consequent in£ammatory response

The Lewis b blood group antigen has been implicated as a putative receptor for Helicobacter pylori in the gastric mucosa. Furthermore, an increased prevalence of duodenal ulcer was found in non-secretors and it has been suggested that secretor status may influence bacterial colonisation density. Other investigators have hypothesised that severity of antral gastritis may be related to colonisation density of the bacterium alone, and that a critical bacterial load is necessary for the development of duodenal ulcer. Our objectives were to investigate whether a relationship existed between host Lewis and ABO blood group phenotype and prevalence of H. pylori infection. In addition we investigated whether bacterial colonisation density and the ensuing inflammatory response was influenced by secretor status and ABO blood group phenotype. The Lewis and ABO blood group phenotype of 207 patients undergoing upper endoscopy was determined. Of these, 136 were secretors and 62 were non-secretors. Forty-five percent of patients were infected with H. pylori . No significant association was found between H. pylori infection and expression of Lewis a or Lewis b blood group antigen. The mean histological density of H. pylori was 1.8(cid:254)0.2 among non-secretors and 1.51(cid:254)0.13 among secretors ( P =0.209), with a mean grade of lymphocytic infiltration significantly greater in H. pylori -infected non-secretors (2.23(cid:254)0.123 vs 1.8(cid:254)0.074; P =0.003). In addition, blood group O non-secretors had a significantly higher grade of lymphocyte infiltration of their gastric mucosa compared to non-O non-secretors (2.53(cid:254)0.133 vs 1.93(cid:254)0.181, P =0.027). These results suggest that although no in vivo relationship exists between H. pylori and preferential adhesion to the putative Lewis b receptor, bacterial colonisation and the ensuing inflammatory response may be influenced at least in part by host expression of ABO and Lewis a blood group antigens. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.
New Technology and Loss of Paid Employment among Older Workers: Prospective Cohort Study

Background: This study investigates the association between the implementation of new technology in the workplace and the subsequent loss of paid employment among older workers. Methods: We estimated the prospective risk of loss of paid employment (register-based) from questions on new technology among 10,320 older workers (≥50 years). To investigate potential differences between work types, analyses were stratified by job function: (1) work with symbols (office, administration, analysis, IT), (2) work with people (people, service, care), (3) work in the field of production (processing, producing or moving things). Results: The introduction of new technology at the workplace reduced the risk of losing paid employment among older workers working with symbols (risk ratio [RR] 0.74, 95% CI 0.72–0.76) and in the field of production (RR 0.83, 95% CI 0.80–0.85), whereas new technology increased this risk among those working with people (RR 1.22, 95% CI 1.19–1.26). Being involved in the introduction of new technology and receiving adequate training in its use decreased the risk of loss of paid employment. Conclusions: Depending on the context, the introduction of new technology at work associates positively as well as negatively with future labour market participation among older workers. Worker involvement and adequate training in the use of new technology seem to be important for retaining workers in the labour market.
Multisystem Proteinopathy Due to VCP Mutations: A Review of Clinical Heterogeneity and Genetic Diagnosis

In this work, we review clinical features and genetic diagnosis of diseases caused by mutations in the gene encoding valosin-containing protein (VCP/p97), the functionally diverse AAA-ATPase. VCP is crucial to a multitude of cellular functions including protein quality control, stress granule formation and clearance, and genomic integrity functions, among others. Pathogenic mutations in VCP cause multisystem proteinopathy (VCP-MSP), an autosomal dominant, adult-onset disorder causing dysfunction in several tissue types. It can result in complex neurodegenerative conditions including inclusion body myopathy, frontotemporal dementia, amyotrophic lateral sclerosis, or combinations of these. There is also an association with other neurodegenerative phenotypes such as Alzheimer-type dementia and Parkinsonism. Non-neurological presentations include Paget disease of bone and may also include cardiac dysfunction. We provide a detailed discussion of genotype-phenotype correlations, recommendations for genetic diagnosis, and genetic counselling implications of VCP-MSP.
Next-Generation Sequencing on Circulating Tumor DNA in Advanced Solid Cancer: Swiss Army Knife for the Molecular Tumor Board? A Review of the Literature Focused on FDA Approved Test

FDA-approved next-generation sequencing assays based on cell-free DNA offers new opportunities in a molecular-tumor-board context thanks to the noninvasiveness of liquid biopsy, the diversity of analyzed parameters and the short turnaround time. It gives the opportunity to study the heterogeneity of the tumor, to elucidate complex resistance mechanisms and to adapt treatment strategies. However, lowering the limit of detection and increasing the panels’ size raise new questions in terms of detection of incidental germline alterations, occult malignancies and clonal hematopoiesis of indeterminate potential mutations. In this review, after a technological discussion and description of the common problematics encountered, we establish recommendations in properly using these FDA-approved tests in a molecular-tumor-board context.
Text Mining and Determinants of Sentiments towards the COVID-19 Vaccine Booster of Twitter Users in Malaysia

Vaccination is the primary preventive measure against the COVID-19 infection, and an additional vaccine dosage is crucial to increase the immunity level of the community. However, public bias, as reflected on social media, may have a significant impact on the vaccination program. We aim to investigate the attitudes to the COVID-19 vaccination booster in Malaysia by using sentiment analysis. We retrieved 788 tweets containing COVID-19 vaccine booster keywords and identified the common topics discussed in tweets that related to the booster by using latent Dirichlet allocation (LDA) and performed sentiment analysis to understand the determinants for the sentiments to receiving the vaccination booster in Malaysia. We identified three important LDA topics: (1) type of vaccination booster; (2) effects of vaccination booster; (3) vaccination program operation. The type of vaccination further transformed into attributes of “az”, “pfizer”, “sinovac”, and “mix” for determinants’ assessments. Effect and type of vaccine booster associated stronger than program operation topic for the sentiments, and “pfizer” and “mix” were the strongest determinants of the tweet’s sentiments after the Boruta feature selection and validated from the performance of regression analysis. This study provided a comprehensive workflow to retrieve and identify important healthcare topic from social media.