Miracle-dz/UniFAR-FTUs huggingface dataset:
Synergistic Role of Endothelial ERG and FLI1 in Mediating Pulmonary Vascular Homeostasis

&NA; Endothelial cell (EC) activation underlies many vascular diseases, including pulmonary arterial hypertension (PAH). Several members of the E‐twenty six (ETS) family of transcription factors are important regulators of the gene network governing endothelial homeostasis, and their aberrant expression is associated with pathological angiogenesis. The goal of this study was to determine whether deficiencies of the ETS family member, Friend leukemia integration 1 transcription factor (FLI1), and its closest homolog, ETS‐related gene (ERG), are associated with PAH. We found that endothelial ERG was significantly reduced in the lung samples from patients with PAH, as well as in chronically hypoxic mice. Functional studies revealed that depletion of ERG or FLI1 in human pulmonary ECs led to increased expression of inflammatory genes, including IFN genes, whereas genes regulating endothelial homeostasis and cell‐cell adhesion were down‐regulated. Simultaneous knockdown of both ERG and FLI1 had synergistic or additive effects on the expression of these genes, suggesting that ERG and FLI1 coregulate at least a subset of their target genes. Functionally, knockdown of ERG and FLI1 induced cell monolayer permeability with a potency similar to that of vascular endothelial growth factor. Notably, stimulation of ECs with Toll‐like receptor 3 ligand poly(I:C) suppressed ERG expression and induced ERG dissociation from the IFNB1 promoter, while promoting signal transducers and activators of transcription 1 (STAT1) recruitment. Consistent with the up‐regulation of inflammatory genes seen in vitro, Erg and Fli1 double‐heterozygote mice showed increased immune cell infiltration and expression of cytokines in the lung. In conclusion, loss of ERG and FLI1 might contribute to the pathogenesis of vascular lung complications through the induction of inflammation.
Myeloid-Derived Suppressor Cells Mediate Inflammation Resolution in Humans and Mice with Autoimmune Uveoretinitis

Resolution of inflammation is an active process that leads to tissue homeostasis and involves multiple cellular and molecular mechanisms. Myeloid-derived suppressor cells (MDSCs) have recently emerged as important cellular components in the resolution of inflammation because of their activities to suppress T cell activation. In this article, we show that HLA-DR−CD11b+CD33+CD14+ human MDSCs and CD11b+Ly6G−Ly6C+ mouse MDSCs markedly increased in patients and mice during and before the resolution phase of autoimmune uveoretinitis. CD11b+Ly6C+ monocytes isolated from autoimmune uveoretinitis mice were able to suppress T cell proliferation in culture, and adoptive transfer of the cells accelerated the remission of autoimmune uveoretinitis in mice. Alternatively, depletion of CD11b+Ly6C+ monocytes at the resolution phase, but not CD11b+Ly6G+ granulocytes, exacerbated the disease. These findings collectively indicate that monocytic MDSCs serve as regulatory cells mediating the resolution of autoimmune uveoretinitis.
Development and Calibration of a Variable Range Stand for Testing Space Micropropulsion Thrusters

Microthrusters are used for microsatellites to provide precise motion control in space by applying very low thrust pulses, which must be properly calibrated on earth. The range of thrust levels required varies from one thruster to another, depending on its use. Thus, microthrusts that stand capable of reading thrusts of multiple ranges, depending on the configuration, are in demand. This paper discusses the features and the calibration method for a typical miniaturized thrust stand. This device is essentially a torsional pendulum whose sensitivity can be varied. As a source of torsion, a piece of wire is used. For the purpose of this paper, the thrust stand was configured to achieve a micro-Newton resolution.
Factors influencing citizen's intention to participate in e-participation: Integrating Technology Readiness on Social Cognitive Theory

The implementation of e-participation in Indonesia nowadays increases along with the rise of e-government. But, participation level in Indonesia is still lack behind other countries. Thus, it is needed to analyze factors influencing citizen's intention to participate electronically. This study examines factors influencing citizen to participate in e-participation service with Social Cognitive Theory (SCT) and Technology Readiness theory. The case study used in this study is LAPOR! , an online public aspiration system in Indonesia. Furthermore, this study is written to validate the use of SCT and extend it with technology readiness theory as technology adoption rate in developing country is not spread evenly. Research data is collected from 410 respondents and analyzed with Partial Least Square — Structural Equation Modelling (PLS-SEM). The empirical outcomes indicate two cognitive factors of SCT (participation self efficacy and outcome expectation personal) have significant impact towards intention to participate. Meanwhile, citizen's technology readiness implies their cognitive, in which influencing their intention to participate. Moreover, the result of this study helps government to improve and maximize e-participation potential so that government transparency can be achieved.
An Unholy Mess: Why ‘The Sanctity of Life Principle’ Should Be Jettisoned

The aim of this article is to present an account of an important element of medical ethics and law which is widely cited but is often confused. This element is most frequently referred to as ‘the principle of the sanctity of life’, and it is often assumed that this language has a religious provenance. However, the phrase is neither rooted in the traditions it purports to represent nor is it used consistently in contemporary discourse. Understood as the name of an established ‘principle’ the ‘sanctity of life’ is virtually an invention of the late twentieth century. The language came to prominence as the label of a position that was being rejected: it is the name of a caricature. Hence there is no locus classicus for a definition of the terms and different authors freely apply the phrase to divergent and contradictory positions. Appeal to this ‘principle’ thus serves only to perpetuate confusion. This language is best jettisoned in favour of clearer and more traditional ethical concepts.
Effects of Noninvasive Skin Stimulation with Microcones on Constipation: A Double-Blinded Controlled Study.

Objective: The use of noninvasive skin stimulation with microcones for constipation is promising. However, no double-blinded study has assessed the effects of this approach on constipation. The goal of this research was to assess the effects on constipation when using noninvasive skin stimulation with microcones. Materials and Methods: This double-blinded, controlled study included 44 subjects, each with a score of 5 points or higher on the Japanese version of the Constipation Assessment Scale (CAS-J). The subjects were randomly divided into 2 groups: (1) a stimulation group (skin-stimulation device with microcones; n = 23; group S) and (2) a placebo group (identical device without microcones; n = 21; group P). The subjects were required to attach the skin-stimulation devices to both cavum conchae with dedicated bandages every day for 2 months by themselves. Main Outcome Measures: This study assessed the severity of constipation, total mood disturbance (TMD) score, and salivary amylase activity (SAA) in each subject. Measurements were obtained before, during, and after the intervention. Results: With regard to the CAS-J score, significant differences were observed in both groups; however, no significant differences were observed between the groups. With regard to the TMD score, significant differences were observed in group S but not in group P. With regard to SAA, there were no significant differences in both groups; however, significant differences were noted after the intervention between the groups. Conclusions: Noninvasive skin stimulation with microcones could relieve constipation and improve TMD scores and SAA activity. This procedure may be used as a self-care option.
Intracranial Pressure and Promontory Vibration With Soft Tissue Stimulation in Cadaveric Human Whole Heads

Hypothesis: Intracranial pressure and skull vibrations are correlated and depend on the stimulation position and frequency. Background: A hearing sensation can be elicited by vibratory stimulation on the skin covered skull, or by stimulation on soft tissue such as the neck. It is not fully understood whether different stimulation sites induce the skull vibrations responsible for the perception or whether other transmission pathways are dominant. The aim of this study was to assess the correlation between intracranial pressure and skull vibration measured on the promontory for stimulation to different sites on the head. Methods: Measurements were performed on four human cadaver heads. A bone conduction hearing aid was held in place with a 5-Newton steel headband at four locations (mastoid, forehead, eye, and neck). While stimulating in the frequency range of 0.3 to 10 kHz, acceleration of the cochlear promontory was measured with a Laser Doppler Vibrometer, and intracranial pressure at the center of the head with a hydrophone. Results: Promontory acceleration and intracranial pressure was measurable for all stimulation sites. The ratios were comparable between all stimulation sites for frequencies below 2 kHz. Conclusion: These findings indicate that both promontory acceleration and intracranial pressure are involved for stimulation on the sites investigated. The transmission pathway of sound energy is comparable for the four stimulation sites.
Coactivation of Cognitive Control Networks During Task Switching

Objective: The ability to flexibly switch between tasks is considered an important component of cognitive control that involves frontal and parietal cortical areas. The present study was designed to characterize network dynamics across multiple brain regions during task switching. Method: Functional magnetic resonance images (fMRI) were captured during a standard rule-switching task to identify switching-related brain regions. Multiregional psychophysiological interaction (PPI) analysis was used to examine effective connectivity between these regions. Results: During switching trials, behavioral performance declined and activation of a generic cognitive control network increased. Concurrently, task-related connectivity increased within and between cingulo-opercular and fronto-parietal cognitive control networks. Notably, the left inferior frontal junction (IFJ) was most consistently coactivated with the 2 cognitive control networks. Furthermore, switching-dependent effective connectivity was negatively correlated with behavioral switch costs. The strength of effective connectivity between left IFJ and other regions in the networks predicted individual differences in switch costs. Conclusions: Task switching was supported by coactivated connections within cognitive control networks, with left IFJ potentially acting as a key hub between the fronto-parietal and cingulo-opercular networks.
Web-Based Alcohol Intervention for Nightclub Patrons: Opposite Effects According to Baseline Alcohol Use Disorder Classification

Background: This study aimed to test the effectiveness of a Web-based intervention in preventing alcohol abuse among nightclub patrons. Methods: A probabilistic sample of the patrons of 31 nightclubs in São Paulo, Brazil, was invited to participate in an online screening using the Alcohol Use Disorders Identification Test (AUDIT). A total of 1057 patrons met the inclusion criteria to participate in the randomized controlled trial, with data collection at 0, 3, 6, and 12 months. At baseline, participants were classified into 2 AUDIT score groups: a “high-risk” group (AUDIT ≥8; 44%) and a “low-risk” group (AUDIT <8; 56%). In both groups, the intervention subgroup was exposed once to a personalized normative feedback screen with information on the participant's alcohol consumption and its potential consequences. Results: After 12 months, no differences were found between the intervention and the control conditions in either risk group. In the “high-risk” group, there were significant reductions of both the AUDIT score and the prevalence of binge drinking (BD) over time in both the control and the intervention subgroups. In addition, an effect of the intervention was observed at 6 months, i.e., there was an estimated 13% reduction in the AUDIT score in favor of the intervention subgroup (odds ratio [OR] = 0.87; 95% confidence interval [CI]: 0.76, 1.00). In the “low-risk” group, both the control patrons and those receiving the intervention had increased AUDIT scores. Conclusion: The results suggest that the time effect of participating in the study may have had a beneficial outcome in reducing harmful drinking among patrons in the “high-risk” group. The intervention is not recommended to the “low-risk” group.
Polymeric cantilever integrated with PDMS/graphene composite strain sensor.

This paper describes the mechanical and electrical characteristics of a polydimethylsiloxane (PDMS) cantilever integrated with a high-sensitivity strain sensor. The strain sensor is fabricated using PDMS and graphene flakes that are uniformly distributed in the PDMS. In order to prepare PDMS/graphene composite with uniform resistance, a tetrahydrofuran solution is used to decrease the viscosity of a PDMS base polymer solution. A horn-type sonicator is then used to mix the base polymer with graphene flakes. Low viscosity of the base polymer solution improves the reliability and reproducibility of the PDMS/graphene composite for strain sensor applications. After dicing the composite into the desired sensor shape, a tensile test is performed. The experimental results show that the composite with a concentration of 30 wt. % exhibits a linear response up to a strain rate of 9%. The graphene concentration of the prepared materials affects the gauge factor, which at 20% graphene concentration reaches about 50, and with increasing graphene concentration to 30% decreases to 9. Furthermore, photolithography, PDMS casting, and a stencil process are used to fabricate a PDMS cantilever with an integrated strain sensor. The change in resistance of the integrated PDMS/graphene sensor is characterized with respect to the displacement of the cantilever of within 500 μm. The experimental results confirmed that the prepared PDMS/graphene based sensor has the potential for high-sensitive biosensor applications.
Cerebral sex dimorphism and sexual orientation

The neurobiology of sexual orientation is frequently discussed in terms of cerebral sex dimorphism (defining both functional and structural sex differences). Yet, the information about possible cerebral differences between sex‐matched homo and heterosexual persons is limited, particularly among women. In this multimodal MRI study, we addressed these issues by investigating possible cerebral differences between homo and heterosexual persons, and by asking whether there is any sex difference in this aspect. Measurements of cortical thickness (Cth), subcortical volumes, and functional and structural resting‐state connections among 40 heterosexual males (HeM) and 40 heterosexual females (HeF) were compared with those of 30 homosexual males (HoM) and 30 homosexual females (HoF). Congruent with previous reports, sex differences were detected in heterosexual controls with regard to fractional anisotropy (FA), Cth, and several subcortical volumes. Homosexual groups did not display any sex differences in FA values. Furthermore, their functional connectivity was significantly less pronounced in the mesial prefrontal and precuneus regions. In these two particular regions, HoM also displayed thicker cerebral cortex than other groups, whereas HoF did not differ from HeF. In addition, in HoM the parietal Cth showed “sex‐reversed” values, not observed in HoF. Homosexual orientation seems associated with a less pronounced sexual differentiation of white matter tracts and a less pronounced functional connectivity of the self‐referential networks compared to heterosexual orientation. Analyses of Cth suggest that male and female homosexuality are not simple analogues of each other and that differences from heterosexual controls are more pronounced in HoM.
Chronic oxidative stress promotes GADD34-mediated phosphorylation of the TAR DNA-binding protein TDP-43, a modification linked to neurodegeneration

Oxidative and endoplasmic reticulum (ER) stresses are hallmarks of the pathophysiology of ALS and other neurodegenerative diseases. In these stresses, different kinases phosphorylate eukaryotic initiation factor eIF2α, enabling the translation of stress response genes; among these is GADD34, the protein product of which recruits the α-isoform of protein phosphatase 1 catalytic subunit (PP1α) and eIF2α to assemble a phosphatase complex catalyzing eIF2α dephosphorylation and resumption of protein synthesis. Aberrations in this pathway underlie the aforementioned disorders. Previous observations indicating that GADD34 is induced by arsenite, a thiol-directed oxidative stressor, in the absence of eIF2α phosphorylation suggest other roles for GADD34. Here, we report that arsenite-induced oxidative stress differs from thapsigargin- or tunicamycin-induced ER stress in promoting GADD34 transcription and the preferential translation of its mRNA in the absence of eIF2α phosphorylation. Arsenite also stabilized GADD34 protein, slowing its degradation. In response to oxidative stress, but not ER stress, GADD34 recruited TDP-43, and enhanced cytoplasmic distribution and cysteine modifications of TDP-43 promoted its binding to GADD34. Arsenite also recruited a TDP-43 kinase, casein kinase-1ϵ (CK1ϵ), to GADD34. Concomitant with TDP-43 aggregation and proteolysis after prolonged arsenite exposure, GADD34-bound CK1ϵ catalyzed TDP-43 phosphorylations at serines 409/410, which were diminished or absent in GADD34−/− cells. Our findings highlight that the phosphatase regulator, GADD34, also functions as a kinase scaffold in response to chronic oxidative stress and recruits CK1ϵ and oxidized TDP-43 to facilitate its phosphorylation, as seen in TDP-43 proteinopathies.
Models of Inflammation: Carrageenan Air Pouch

The subcutaneous air pouch is an in vivo model that can be used to study the components of acute and chronic inflammation, the resolution of the inflammatory response, the oxidative stress response, and potential therapeutic targets for treating inflammation. Injection of irritants into an air pouch in rats or mice induces an inflammatory response that can be quantified by the volume of exudate produced, the infiltration of cells, and the release of inflammatory mediators. The model presented in this unit has been extensively used to identify potential anti‐inflammatory drugs. © 2016 by John Wiley & Sons, Inc.
Burden associated with smoking as a suicidal risk factor in an Australian sample of patients with psychosis

Objective: We aim to describe the burden associated with smoking as a risk factor for suicidal behaviors among patients with psychosis. Method: We calculated the Population Attributable Risk (PAR) associated with smoking and other known modifiable risk factors for suicidal behaviors for participants (N=1812) who took part in the Australian Survey of High Impact Psychosis (SHIP). A combined PAR for the seven identified modifiable risk factors was calculated. Results: Smoking contributed to 21% of the suicidal behaviors in this sample; together, the seven factors contributed to 86% of all attempted suicide in this cohort. Conclusions: Smoking may exert a role either directly or indirectly. Evidence indicates that smoking is associated with psychological, biochemical and neurobiological changes that are in turn implicated in smoking. Suicidal behaviors are nevertheless complex behaviors needing multi-faceted approach.
Tracking and counting of vehicles for flow analysis from urban traffic videos

Among the major problems faced by urban centers there is traffic congestion. This problem comes from the growing number of vehicles on the streets and has already become the subject of several researches seeking for solutions to it. Among the mechanisms that allow congestion reduction is traffic control, which requires metrics that enable traffic analysis in real time. To determine the flow of vehicles the widely used mechanism is the counting of occurrence of vehicles on a street, which is usually performed from sensors (e.g. magnetic or thermal). However, these approaches have a rather high installation and maintenance difficulty. Thus, the objective of this paper is to present a mechanism capable of counting from video images. To accomplish this task it is used image processing resources that do not require large computational power, thus allowing the mechanism to be easily coupled to common transit systems. The result obtained has an accuracy of more than 90 % in videos of urban traffic cameras.
The effects of thematic relations on picture naming abilities across the lifespan

ABSTRACT A picture–word interference paradigm tracked patterns of activation during picture naming in 87 individuals (age range 17–80 years old). Distractor words were presented at stimulus onset asynchronies (SOAs) of −200, −100, and 0 ms bearing a has a-, location, or no relationship to the picture. Analyses of group naming reaction times revealed significant facilitation effects for both semantic relation types for all age groups. Analyses of temporal patterns of activation revealed significant effects primarily at SOAs of −200 and −100 ms. These findings provide evidence that both thematic relations are particularly salient in how semantic knowledge is organized, and that the patterns of effects from these semantic relations remain the same as one ages.
Modified bat algorithm with cauchy mutation and elite opposition-based learning

Metaheuristics can be used to solve optimization complex problems because they offer approximate and acceptable solutions. In recent years, nature has been a source of inspiration for many computer scientists when proposing new metaheuristics such as the algorithms inspired by swarm intelligence. They are based on the behavior of animals that live in groups such as birds, fishes and bats. In this context, Bat Algorithm (BA) is a recent metaheuristic inspired by echolocation of bats during their flights. However, a problem that this algorithm faces is the loss of the ability to generate diversity and, consequently, the chances of finding the global solution are reduced. This paper proposes a modification to the original BA using two methods known as Cauchy mutation operator and Elite Opposition-Based Learning. The new variant aims generate diversity of the algorithm and increases its convergence velocity. It was compared to the original BA and another variant found in the literature. For this comparison, the proposed variant used four benchmark functions, during 30 independent runs. After the experiments, the superiority of the new variant is highlighted when the results are compared to the original BA.