Miracle-dz/UniFAR-FTUs huggingface dataset:
CYANOBACTERIAL ACCLIMATION TO CHANGING ENVIRONMENTAL CONDITIONS Roles for group 2 sigma factors in Synechocystis sp. PCC 6803

In cyanobacteria gene expression is mainly controlled at the level of transcription initiation. The RNA polymerase holoenzyme (RNAP) is composed of a catalytic core and a sigma factor ( ) that recognizes promoter elements. Bacterial genomes encode several different factors and the exchange of one factor to another is thought to be a major determinant in changing the gene expression pattern. The cyanobacterium Synechocystis sp. PCC 6803 encodes nine factors. SigA is the group 1 factor, SigB, SigC, SigD and SigE are group 2 factors and SigF, SigG, SigH and SigI belong to group 3. Structural modelling of the RNAP revealed that the three-dimensional structures of the group 2 factors are very similar. Single, double and triple inactivation strains were constructed to study the roles of the group 2 factors. Similar growth and photosynthesis in all inactivation strains under standard conditions (BG-11 medium pH 7.5, 32 C, continuous light 40 mol photons m s, air level CO2) indicated that the group 2 factors are nonessential for growth under standard conditions. Group 2 factors were found to be important for acclimation to environmental stress conditions. SigB and SigC have key roles in acclimation to heat stress. The SigB factor was required for survival in short extreme heat stress and for acquired thermotolerance. SigB was a positive regulator of the hspA gene, which encodes a heat shock protein. SigC in contrast was crucial both in longand short-term heat stress and for acquired thermotolerance. High temperatures diminish the availability of inorganic carbon. DNA microarray analysis and physiological measurements suggest a role for the SigC factor in the regulation of carbon metabolism during heat acclimation. Optimal acclimation to salt and hyperosmotic stress involved many group 2 factors. SigB was crucial for growth in both salt and hyperosmotic stress while SigC played a minor role. The SigE factor had a role only in salt-induced osmotic stress. Acclimation to different light conditions was regulated by the SigB and SigD factors together. Strains lacking the SigD factor were sensitive to light on agar plates. When growth light intensity was doubled, all strains with simultaneously inactivated sigB and sigD genes were not able to take full advantage of a greater light availability. In addition, the sigBD strain was more vulnerable to high light stress than the control or single inactivation strains due to a deficient photosystem II repair cycle. The SigB and SigD factors have partial functional redundancy probably because they have highly similar structures.
Housing, Husbandry, and Clinical Techniques for Crayfish (Procambarus clarkii) used in Biomedical Research

Crayfish (Decapoda: Astacoidea & Parastacoidea), are amongst the few animals that have stem cells within hemolymph, with the capacity to continuously produce differentiated neuronal structures throughout life. As crayfish and other invertebrates continue to become common models in research to study human disease, it is vital that we develop universal laboratory standards and guidelines on housing and husbandry practices. This publication presents introductory data on housing, husbandry, hemolymph collection and statistically supported anesthesia trials, to support future research endeavors. Evaluation of housing, husbandry, clinical and anesthetic techniques in Procambarus clarkii maintained in a biomedical research setting were performed. An option for hemolymph collection, in the area termed, the Ventral Coelomic Hemolymph Collection Zone (VCHCZ) is presented as a technique to assess hemocytes. Additionally, Wright Giemsa stained slides of crayfish hemolymph were evaluated by a board-certified veterinary clinical pathologist for interpretation and confirmation of hemolymph collection. The housing and husbandry experiments were performed over a duration of 37 days. Mortality rates and physical health assessments were performed. The following water quality parameters were concurrently evaluated: temperature, light cycle, pH, KH, GH, conductivity, total dissolved solids, salinity, ammonia, nitrite, and nitrate. Anesthetic techniques were evaluated between four experimental groups: (A) immersion in buffered MS-222 (50 mg/L), (B) immersion in buffered MS-222 (150 mg/L) (C) immersion in Propofol (65 mg/L) and (D) Propofol injection (100 mg/kg) into VCHCZ. Housing and husbandry techniques were validated with 0% mortality and normal species-specific behaviors were observed. MS-222 immersion had no observable effect on crayfish. Propofol immersion (65 mg/L) created sedative effects allowing for appropriate handling. Propofol injection (100 mg/kg) into VCHCZ successfully created a deep anesthetic plane that would allow for more invasive or surgical procedures, without adverse effects during or after recovery.
Beneficial effects of hedonic and eudaimonic motivations on subjective well-being in adolescents: a two-wave cross-lagged analysis

ABSTRACT Hedonia and eudaimonia have been proposed as pathways to well-being. However, the direction of the causal relationship between them remains unclear. This study examined if happiness motivations may be resources leading to a happy life, or vice versa. Cross-lagged associations between happiness motivations (i.e. hedonic motivation and eudaimonic motivation) and subjective well-being (i.e. positive affect, negative affect, and life satisfaction) were tested. Four hundred and ninety-four Chinese adolescents (233 males; mean age 13.26 ± 0.70 years) participated in a two-wave paper and pencil study. The cross-lagged analysis showed that eudaimonic motivation was a significant predictor of positive affect and life satisfaction over time. No cross-lagged effects between eudaimonic motivation and negative affect, or between hedonic motivation and subjective well-being components were established. The results highlight the predictive power of eudaimonic motivation on adolescents’ subjective well-being later on.
Big Data Analysis of COVID-19 Mitigation Policy in Indonesia: Democratic, Elitist, and Artificial Intelligence

In response to mitigate COVID-19 local transmission, the Indonesian government has launched several regulative policies. This paper aims to analyze whether the decision-making process of those regulative policies is closed to a democratic or elitist approach and whether the government has adopted Artificial Intelligence (AI) in these processes. We focus on large-scale social distancing (PSBB), homecoming restriction in the Eid Mubarak (Mudik), and new-normal life policy case studies. The research has shown that elite roles are quite dominant with little consideration for public consultation and little attention to the use of AI to consider public preferences. The data were collected from two well-known national online media, which are nasional.kompas. com and tempo.co, while the big data mining and analysis have been applied through N-Vivo 12 software. We argue that although the elitist paradigm can be recognized as an efficient approach in the pandemic and emergency, the decision-makers can accommodate public preferences if they can get insights from online media news with the help of AI technology.
Twelve new records of Coral associated fish from the Saint Martin’s island based on morphometric and molecular approaches

The study was conducted to know the diversity of coral associated fishes of the Saint Martin Island from December 2017 to April 2019. A total of 46 coral associated marine fish species were recorded, among them 12 species; sunajtuL iughcliug , Lutjanus russellii, Parupeneus indicus, Siganus sutor, Thalassoma lunare, Gazza achlamys, Pomadasys andamanensis, Pomadasys kaakan, Lethrinus crocineus, Argyrosomus regius, Istigobius ornatus and Cryptocentrus cyanotaenia under six family were newly recorded from this Island. We assume that there are more species to be explored and need proper management strategy for conservation of coral reef and its associated biodiversity.
Determinants of smoking cessation counseling favorable practice for primary care physicians: A cross-sectional study from Palestine

Background: Smoking cessation counseling is not well established in Middle East countries including Palestine. This study assesses the practice of smoking cessation counseling among primary healthcare (PHC) physicians in Palestine and its determinants. Methods: Over the period from April to September 2019, general practitioners, family medicine physicians, obstetricians and gynecologists, and dentists working at the PHC Centers in Palestine were approached through a cross-sectional design using a self-reported questionnaire. Proportionate stratified random sampling approach has been used. Sociodemographic, medical background, smoking cessation counseling training, smoking history, practice, knowledge, attitude, and confidence were evaluated. The proportion of favorable practice has been determined and its relationship with all variables has been evaluated. Ethical approval was received from the National University of An-Najah. Results: 295 physicians were enrolled in the study with an 86% response rate. 75.5% (n = 222) were males with a mean age of 39.9 ± 9 years. The majority were General Practitioners (76.5%, n = 252). Most of them (84.3%, n = 215) had not received any training. The favorable practice was seen in 36.6% (95% CI 31–41%). Attitude is the main predictor of favorable practice (P value = 0.002, adjusted OR = 1.1). Conclusion: Palestinian PHC physicians have a low level of counseling for smoking cessation. Given the efficacy of counseling for smoking cessation and the high level of the tobacco epidemic in the area, health policy to ensure all healthcare personnel-especially physicians-receive training in cessation counseling and call for a public health campaign for control of tobacco should be taken into account.
Status Quos Are Made to be Broken: The Roles of Transformational Leadership, Job Satisfaction, Psychological Empowerment, and Voice Behavior

Employee voice behavior has attained significant attention in contemporary research due to its positive consequences for both workers and employers. Drawing on the social exchange theory, this study examined the mediating role of job satisfaction and psychological empowerment on the relationship between transformational leadership and employee’s voice behavior. Data were collected through survey questionnaires by utilizing a three-wave time-lagged study design from employees from diverse private and public sector organizations in Pakistan. The parallel multiple mediation is tested through Hayes’s process macro. The results indicate that job satisfaction and psychological empowerment partially mediate the relationship between transformational leadership and employee’s voice behavior. Further analysis depicts that both job satisfaction and psychological empowerment leveraged under transformational leadership act as parallel mediators and have no statistical significant difference between them. The theoretical and managerial implications are discussed.
First Impressions Stick: Market Entry Strategies and Category Priming in the Digital Domain

The rise of the “digital age” presents unique challenges for firms entering new markets and deciding “where” to compete – a pivotal topic in corporate strategy. Particularly, it is not clear what the opportunities and implications are for digital new entrants as they position their disruptive business offerings in the category system, in particular vis-a-vis non-market stakeholders. In this article, we qualitatively investigate how two icons of the “sharing economy,” Uber and BlaBlaCar, pursued two distinct categorization strategies which were incumbent-focused and economic versus emergent-focused and non-economic. Our longitudinal comparative case study reveals how digital new entrants, through self-categorization, can enduringly impact the nature of the responses of non-market stakeholders. The mechanism at play is category priming – the process of directing stakeholders’ selective attention towards, or away from, the commonalities shared with a specific market category – and its stickiness over time. In particular, the distinct categorization strategies primed stakeholders to focus (Uber) or not focus (BlaBlaCar) on similarities between the entrant and an established category, which triggered polarized responses from media and regulators and resulted in a “sticky” association regardless of repositioning efforts. Our contribution dissects the constituents and consequences of these strategies and discusses implications for future research on digital market entry, strategic categorization, and business models.
Graphene-facilitated photocatalysis for water detoxification and disinfection

Graphene with a high electrical conductivity is a suitable material for applications where electron transfer plays in an important role. Our recent research data show that graphene sheets functionalized with metals, metal oxides, metal chloride, and carbon nanotubes display very interesting photocatalytic properties in removing non-biodegradable dyes under visible light irradiation and killing microorganisms. A completely new photocatalytic mechanism is proposed for photocatalytic degradation of dyes - it is the dye that is excited first, followed by rapid transfer of the excited electron through graphene to an electron reservoir (e.g., metal nanoparticles) and subsequent trapping by adsorbed O2 to produce various reactive oxygen species (ROS).
Effect of Calcination Temperature over the Performance of Mn-Ce-O on the Catalytic Ozonation of Olive Mill Wastewater

ABSTRACT The effect of calcination temperature on Mn-Ce-O 70/30 characteristics, activity and stability was investigated when this catalyst was applied on the catalytic ozonation of a phenolic mixture. Two crystalline phases (cubic CeO2 and tetragonal Mn3O4) were observed by X-ray diffraction analysis, which also showed an increase of crystallinity with temperature. The material prepared at 300 ºC was the most efficient regarding TPh (100%), TOC (80%) and COD (62%) removal, as well as revealed to be the one leading to the lowest amount of Mn leached. The generalized kinetic model (GKM) was able to describe mineralization during this catalytic process with relative errors of ±10%.
Bill Text and Agenda Control in the US Congress

The study of agenda power has largely been the study of negative agenda power. But standard measures of negative agenda power are insufficient to measure the majority’s agenda choices: they only consider a small proportion of bills, only detail how often negative agenda power fails (rather than succeeds), and cannot help us understand positive agenda power. To understand the incentives and strategies of agenda decision-making, then, we must know about members’ preferences on all bills. I develop an approach to estimate members’ preferences on all bills, by generating quantitative characterizations of the policy content in each bill. I use the resulting estimates to examine both positive and negative agenda power using all bills and to directly compare levels of agenda power between chambers of the US Congress. While I find similarly strong negative agenda control in both chambers, I find substantially stronger positive agenda control in the House than the Senate.
Success Factors of Social Media Applications (SMA): Case Study among UUM Students

Social media application (SMA) shows several important functions that causing theincrement of usage among mobile application or mobile app users, especially among18 to 28 years-old users. This causing several developers to create their own SMA thathave been targeted to mobile app users. However, only several SMA managed tobecome popular and successful in term of usage, leaving other unpopular SMA in thelower rank of the Google PlayStore. SMA created by developer in Malaysia face thesame situation as mentioned before where those SMA were supposed to attractMalaysian mobile users more. To assess this situation, this study aims to identify thesuccess factors of SMA usage and develop a set of metric based on the success factorsusing research model that have been developed in the past. Information SystemSuccess Model (ISSM) were studied and chosen as the reference model for this studybecause the model is suitable and have been used by other researchers in studiesregarding social media and SMA. ISSM contains several success factors like systemquality, service quality and information quality that affect the user satisfaction and useof a system, where this model were modified in this study with the addition ofnetworking quality and perceive privacy factors. This study were conducted on 380Universiti Utara Malaysia (UUM) students and after analysing the data collected, allproposed success factors except of service quality were found to have a positive impacttowards user satisfaction and usage. The success factors were included in the metricdesign and the metric were presented in an evaluation form for SMA developer inMalaysia to evaluate and applied the metric in their SMA.
Decompositions of Taylor diagram and DISO performance criteria

Taylor diagram has been frequently used to evaluate climate or hydrology models or data. A Taylor diagram summarizes three frequently used metrics including correlation coefficient (CC), standard deviation (STD), and centred root mean square error (RMSEc). Although these three metrics are relevant metrics for some applications, in some cases, additional indicators are needed, which calls for a new method. This study firstly addressed the short comments about the distance between indices of simulation and observation (DISO) described in a previous study. Secondly, the number of statistical metrics of DISO is extended from 3 to more than 3. The statistical metrics of the expanded DISO are more flexible than the Taylor diagram which uses the three fixed metrics. Thirdly, the current work compares the Taylor diagram with the expanded DISO in terms of their theoretical bases, revealing the advantage of DISO in terms of its flexibility in the selection of different types of metrics, and its suitability as an effective single metric to express a model's or dataset's overall quality. The power and flexibility of the expanded DISO are discussed.
Segregation Distortion for Male Parents in High Density Genetic Maps from Reciprocal Crosses between Two Self-Incompatible Cultivars Confirms a Gametophytic System for Self-Incompatibility in Citrus

Self-incompatibility is an important evolutionary feature in angiosperms and has major implications for breeding strategies in horticultural crops. In citrus, when coupled with parthenocarpy, it enables the production of seedless fruits in a mono-varietal orchard. A gametophytic incompatibility system with one S locus was proposed for citrus, but its molecular mechanisms remain the subject of debate. The objective of this work was to locate the S locus by the analyzing segregation distortion in reciprocal crosses of two self-incompatible citrus sharing one self-incompatible allele and to compare this location with previously published models. High density genetic maps of ‘Fortune’ mandarin and ‘Ellendale tangor’ with, respectively, 2164 SNP and 1467 SNP markers, were constructed using genotyping by sequencing data. They are highly syntenic and collinear with the clementine genome. Complete rejection of one allele was only observed in male segregation in the two parents and in only one genomic area, at the beginning of chromosome 7 of the clementine reference genome. Haplotype data in the area surrounding the theoretical S locus were in agreement with previously proposed S genotypes. Overall, our results are in full agreement with the recently proposed gametophytic S-RNase system with the S locus at the beginning of chromosome 7 of the clementine reference genome.
Courtship rhythm in Nasonia vitripennis is affected by the clock gene period

The clock gene period (per) is a regulator of circadian rhythms but may also play a role in the regulation of ultradian rhythms, such as insect courtship. Males of the parasitoid wasp Nasonia vitripennis court females by performing series of head movements (‘head-nods’) and wing vibrations within repeated cycles. The pattern of cycle duration and head-nod number is species-specific and has a genetic basis. In this study, the possible involvement of per in regulating Nasonia courtship rhythms was investigated in a southern and northern European strain that differ in number and timing of courtship components. Knockdown of per via RNA interference (RNAi) resulted in a shortening of the circadian free running period (tau) in constant darkness (DD), and increased both the cycle duration and the number of head-nods per cycle in both strains. These results point at a role of per in the regulation of ultradian rhythms and male courtship behaviour of N. vitripennis and may contribute to resolving the controversy about the role of per in insect courtship behaviour.
About nilalgebras satisfying (xy)2 = x 2 y 2

Abstract A classical problem in nonassociative algebras involves the existence of simple finite-dimensional commutative nilalgebras. In this paper, we study the class Ω of nonassociative algebras satisfying the identity over a field of characteristic different from 2 and 3. We show that every unitary algebra in Ω is associative. Next, we prove that each prime algebra in Ω is either associative or its center vanishes. For nilalgebras, we obtain that every nilalgebra in Ω is an Engel algebra. Finally, we show that every commutative nilalgebra in Ω of nilindex 4 over a field of characteristic not 2, 3 and 5 is solvable of index
A systematic review of online parent-implemented interventions for children with neurodevelopmental disorders

ABSTRACT Online parent-implemented interventions may increase treatment accessibility and reduce health care services burden by enabling parents of children with neurodevelopmental disorders (NDD) to implement treatments at home. This systematic review identified and evaluated the quality of evidence for the efficacy of online parent-implemented interventions for children with Autism Spectrum Disorder (ASD), Attention-Deficit/Hyperactivity Disorder (ADHD), Cerebral Palsy (CP), and Fetal Alcohol Spectrum Disorder (FASD). Twelve interventions were identified targeting a range of behaviors (ASD = 8, ADHD = 3, FASD = 1). Preliminary evidence suggests that online parent-implemented interventions are promising for children with these diagnoses, but require more rigorous, large-scale research as study quality was poor overall.