Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of Functional Ribozymes in Bacteria

A trans-acting hammerhead ribozyme, designed to cleave mRNA from the human immunodeficiency virus-1 integrase gene, was expressed in Escherichia coli from a plasmid-borne gene. Expression of the ribozyme at the same time as expression of integrase led to the disappearance of integrase mRNA and prevention of integrase production. A comparable antisense RNA was much less effective. The elements of the ribozyme gene are described to facilitate construction of ribozymes directed at other targets. Procedures for modeling ribozyme secondary structure, testing ribozyme activity, and inserting ribozyme genes into expression vectors are discussed.
Seleção de isolados de Beauveria bassiana para o controle biológico da broca-do-café, Hypothenemus hampei (Ferrari) (Coleoptera: Scolytidae)

Hypothenemus hampei (Ferrari) (Coffee Berry Borer) is one of the most important coffee pests. Its control is carried out mainly using synthetic chemical products, which contaminate the environment, food and farmers. The entomopathogenic fungus Beauveria bassiana is a natural enemy of coffee berry borer and presents potential for biological control. With the objective to select strains of B. bassiana for the management of H. hampei, the virulence of 61 strains, from diverse hosts and geographic regions, were tested. The selection was carried out in two phases: in the first phase 11 strains, with confirmed mortality above 60%, were selected. In the second phase, for the 11 preselected strains, we determined: LC50, sporulation rate (confirmed mortality/total mortality) and conidia production on H. hampei cadavers. The CG425 strain presented the greater total and confirmed mortality, highest sporulation rate and CL50 = 2.5 x 106 conidia/ml and CB102 strain, presented highest conidia production on insects, 11.6 x 106 conidia/insect. These isolates present height potential to be used in biological control programs of coffee berry borer with B. bassiana.
Does employment protection affect unemployment? A meta-analysis

Despite extensive research efforts, the magnitude of the effect of employment protection legislation (EPL) on unemployment remains unclear. Existing econometric estimates exhibit substantial variation, and it is therefore difficult to draw valid conclusions. This paper applies meta-analysis and meta-regression methods to a unique data set consisting of 881 observations on the effect of EPL on unemployment from 75 studies. Once we control for publication selection bias, we cannot reject the hypothesis that the average effect of EPL on unemployment is zero. The meta-regression analysis, which investigates sources of heterogeneity in the reported effect sizes, reveals the following main results. First, the choice of the EPL variable matters estimates that build on survey-based EPL variables report a significantly stronger unemployment-increasing impact of EPL than estimates developed using EPL indices based on the OECD’s methodology, where the latter relies on coding information from legal provisions. Second, we find that employment protection has a small unemployment-increasing effect on female unemployment, compared with a zero impact on total unemployment. Third, using multi-year averages of the underlying data tends to dampen the unemployment effects of EPL. Fourth, product market regulation is found to moderate the effect of EPL on unemployment. Disclaimer Funding from the Austrian Ministry of Social Affairs is gratefully acknowledged.
Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China

The causative agent was isolated from diseased turbots (Scophthalmus maximus) stricken by a high-mortality outbreak of bacterial septicaemia occurring in a mariculture farm in Yantai, a northern coastal city of China. Seven pure isolates, namely EH-15, EH-103, EH-107, EH-202, EH-203, EH-305 and EH-306, belonged to Edwardsiella tarda. The phenotypic features of the cultures were analysed extensively. Three of the isolates showed high 16S rDNA sequence similarities with E. tarda sequence (GenBank accession no. EF467289). However, unlike the E. tarda ATCC 15947, all the isolates, except EH-15, contained a novel large plasmid sized about 23.7 kb. Furthermore, pathogenicity of the isolates was addressed by experimental challenges with fish models. The isolates exhibited strong virulence to swordtail fish with LD50 ranging between 3.8 × 103 and 3.8 × 105 CFU g−1, and EH-202 displaying the lowest LD50 value among them. Antibiotic susceptibilities of E. tarda isolates were assayed. Compared with E. tarda ATCC 15947, the isolates displayed strong resistance to chloramphenicol, and the probable dominant chloramphenicol resistance determinant was cat III. Depicting the main biological properties of turbot-borne E. tarda strains in China, the study provided useful information for further unveiling their pathogenic mechanisms.
Effect of Glass Fiber Post Diameter on Fracture Resistance of Endodontically Treated Teeth

Objective: To determine the fracture resistance of endodontically treated teeth restored with three different diameters of glass fiber posts and metal-ceramic crowns. Material and Methods: Thirty human maxillary canines were selected and subjected to root canal therapy. The teeth were randomly divided into three groups of glass fiber posts with 1.4 mm diameter (Group I), 1.6 mm diameter (Group II), and 2.0 mm diameter (Group III). The teeth were restored with metal-ceramic crowns and subjected to the compressive load applied at 45° angle to the longitudinal axis until fracture. The mode of failure was determined. Data were analyzed using ANOVA, followed by Tukey's post hoc multiple comparisons test (p<0.05) . Results: The mean fracture resistance of groups I, II and III was 574 ± 91.2 N, 617 ± 85.21 N and 467 ± 99.43 N, respectively. No significant difference was noted between groups I and II, while the fracture resistance was significantly different between groups I and III (p<0.05) and groups II and III (p<0.05). No case of post fracture alone occurred in any group . Conclusion: The diameter of glass fiber posts can affect the fracture resistance of teeth. Based on the results, increasing the diameter of the post up to 1.6 mm may increase the fracture resistance of root, although excessive diameters are not recommended.
The value of genetic resources in agriculture: a meta-analysis assessing existing knowledge and future research needs

The protection of genetic resources in agriculture is an important aspect of biodiversity conservation. Knowledge of the value of genetic resources can contribute to determining the appropriate focus and extent of conservation. This study reviewed and summarised literature on the economic value of genetic resources using meta-analysis. Altogether, 22 studies were used to describe current knowledge on the value of genetic resources. Furthermore, 14 studies with 93 value observations were examined with a meta-regression model to identify variables that explain the willingness-to-pay (WTP) for or willingness-to-accept (WTA) loss of genetic resources. Grain genetic resources were ascribed lower value compared to animal genetic resources (AnGR) and agrobiodiversity, and the values of breeds or varieties and conservation programmes were higher than the value of individual attributes. Future research should address the gaps in knowledge that are relevant for policy-making. This particularly includes improving knowledge on the value of plant genetic resources (PGR), obtaining value estimates for maintaining genetic diversity in Europe and the United States, estimating the relative magnitude of use and non-use values and determining the value consumers place on genetic resources and diversity in agriculture. An extensive database with valuation literature on genetic resources that fulfils the requirements for benefit transfer is essential to utilise value information more efficiently in decision-making situations.
STRANGE ATTRACTORS FOR THE LOZI MAPPINGS

into itself. Therefore. one can hope that ( 1 ) i t will be easier to investigate the Lozi mappings than the Hiinon ones and (2) for certain values of parameters. the Hknon mappings will be conjugate to some Lozi mappings. Although it may happen that the last statement is false, there should be some connection between piecewise linear and quadratic mappings of the plane. In the present paper. I prove that, for some set of values of the parameters, the Lozi mappings have strange attractors. To do this, I find a trapping region and then prove that the mapping has a hyperbolic structure. These facts, along with some geometrical properties of the mapping, enable me to prove that the intersection of the images of the trapping region is ;I strange attractor. Since the mapping is not everywhere differentiable. its hyperbolic structure can be understood only as the existence of a hyperbolic splitting a t those points a t which it may exist. This splitting cannot be extended to a continuous one on the whole plane. The usual meaning of the notions of stable and unstable manifolds also has to be changed a little bit. They are broken lines, and therefore not manifolds. Nevertheless, I prefer to call them (un)stable manifolds rather than (un)stable sets. I show that they exist a t almost all points of the trapping region. In the last section, I discuss briefly what happens for other values of parameters.
Fertilities from mating and artificial insemination with frozen-thawed spermatozoa by indexing LH surge in beagle bitches.

The purpose of this study was to examine fertilities from mating and artificial insemination (AI) with frozen-thawed spermatozoa by indexing the luteinizing hormone (LH) surge with the Canine Ovulation Timing Test (ICG Status-LHTM) in beagle bitches. Eleven 2-8-year-old multiparous dogs were used for this experiment and blood was collected daily at 6-15 days after the onset of proestrus. Each serum sample was then used for detection of the LH surge. Five dogs were mated at 5 days after the LH surge. Whelping was successful in all dogs and litter size was 5.8 ± 1.9 (Mean ± SD). The remaining 6 dogs were artificially inseminated with frozen-thawed spermatozoa at 4, 5 and 6 days after the LH surge. Sperm motility after thawing was 49.6 ± 3.6%. All dogs whelped and litter size was 4.0 ± 3.0. These results suggest that fertilities from mating and AI with frozen-thawed spermatozoa are satisfactory by indexing the LH surge with the Canine Ovulation Timing Test in dogs and further demonstrate that the optimal period for insemination is around 5 days after the LH surge.
Correlates of Perceptions of Bullying at School among First Nations Youth Living Off Reserve

This study described perceptions of bullying as a school characteristic and associations with school, academic, and health characteristics among a representative sample of First Nations high school students living off reserve in Canada using data from the 2012 Aboriginal Peoples Survey. Almost 4 in 10 of First Nations youth living off reserve perceived bullying as a problem at their schools. A perceived climate of bullying co-occurred with other negative school climate characteristics such as racism, violence, and the presence of alcohol and drugs. First Nations youth living off reserve who perceived bullying as a problem at school reported higher psychological distress and a higher prevalence of suicidal ideation, even after controlling for the effects of youth sex, age, and household income. These findings highlight the need to focus on school characteristics as perceived by youth to improve school climate and youth health.
ATP-OIE: An Autonomous Open Information Extraction Method

This paper describes an innovative Open Information Extraction method known as ATP-OIE1. It utilizes extraction patterns to find semantic relations. These patterns are generated automatically from examples, so it has greater autonomy than methods based on fixed rules. ATP-OIE can also summon other methods, ReVerb and ClausIE, if it is unable to find valid semantic relations in a sentence, thus improving its recall. In these cases, it is capable of generating new extraction patterns online, which improves its autonomy. It also implements different mechanisms to prevent common errors in the extraction of semantic relations. Lastly, ATP-OIE was compared with other state-of-the-art methods in a well known texts database: Reuters-21578, obtaining a higher precision than with other methods.
VITAMINS IN RABBIT NUTRITION : LITERATURE REVIEW AND RECOMMENDATIONS

Vitamins are classified in a total of 13 substances or groups of substances. Four vitamins are fat-soluble (vitamin A, D E, and K) and the nine others (vitamins of the B-complex and vitamin C) are water-soluble. The water-soluble vitamins and vitamin K are normally synthesised by the rabbit's digestive flora; but in cases of high risk of digestive disorders (e.g. just after weaning), dietary supplementation may be advisable. In addition, a vitamin C supplementation (25 to 30 mg per rabbit and per day) can help the animal in stress situations (heat stress, .). The vitamin A requirement is largely satisfied if the diet contains 1 O 000 1 U vitamin A per kg or 30 ppm of p-carotene. Additional provision of vitamin A is unnecessary for growing rabbits and may be toxic to the foetus in pregnant does. The dietary recommended vitamin D level is 800 to 1000 IU/kg. lf the dietary concentration is greater than 2000 IU/kg, abnormal calcification of soft tissues (aorta, kidneys, .) is generally observed. The recommendation for vitamin E is 50 mg/kg. A diet that contains only 15 mg/kg of vitamin E induces deficiency symptoms (muscular dystrophy, sudden death, reproduction disorders, .). A massive introduction of vitamin E is not toxic but may improve rabbit meat shelf-life, by reduction of the rate of lipid oxidation.
Peripheral neurons and peripheral neurosecretion in the stick insect, Carausius morosus

Light and electron microscope studies of peripheral neurons on nerves in the abdominal segments of the stick insect (Carausius morosus) reveal distinct differences between a group of eight or nine that are probably sensory and a group of about four that are actively neurosecretory. In addition to the characteristic granules produced by these cells four other types of neurosecretory granules have been identified. A map of the distribution of the five types has been constructed from electron-micro-graphs of sections of the main peripheral nerves. In each main abdominal segment there are also approximately 48 pairs of sub-epidermal neurons.
Biological and Chemical Study of Some Soft Corals and Sponges Collected in Mauritian Waters

Thirty-seven samples of soft corals and sponges collected in July 2004 between the north and west coasts of Mauritius at depths varying from 6-26 metres have been biologically screened against inhibition of urchin egg cell division, brine shrimp lethality test, antiacetylcholinesterase test and against two bacteria Escherichia coli and Micrococcus luteus . The sponges Rhabdastrella sp. aff. providentiae (Dendy) (M03 SP3 FF) and Pericharax heterorhaphis (M03 SP4 FF) were found to be active against Escherichia coli and Micrococcus luteus respectively at a concentration of 1 μg/mL and the sponge Liosina paradoxa (M03 SP3 BC) exhibited anti-acetylcholinesterase activity. Fractionation and purification of the crude extract of the soft coral M03 SC1 GB using flash, open column and high performance liquid chromatography resulted in the isolation of one secondary metabolite (Guaianediol). Seventeen sponges have also been taxonomically identified. Keywords: Soft corals, sponges, taxonomy, secondary metabolite, biological screening, Mauritius West Indian Ocean Journal of Marine Science Vol. 5 (2) 2006: pp. 115-122
102 Genome engineering nucleases derived from GIY-YIG homing endonucleases

Efficient targeted manipulation of complex genomes requires highly specific endonucleases to generate double-strand breaks at defined locations (Bibikova et al., 2003; Bogdanove and Voytas, 2011). The predominantly engineered nucleases, zinc-finger nucleases (ZFNs), and TAL effector nucleases (TALENs) use the catalytic domain of FokI as the nuclease portion. This domain, however, functions as a dimer to nonspecifically cleave DNA meaning that ZFNs and TALENs must be designed in head-to-head pairs to target a desired sequence. To overcome this limitation and expand the toolbox of genome editing reagents, we used the N-terminal catalytic domain and interdomain linker of the monomeric GIY-YIG homing endonuclease I-TevI to create I-TevI-zinc-fingers (Tev-ZFEs), and I-TevI-TAL effectors (Tev-TALs) (Kleinstiver et al. 2012). We also made I-TevI fusions to LAGLIDADGs homing endonucleases (I-Tev-LHEs). All the three fusions showed activity on model substrates on par with ZFNs and TALENs in yeast-based recombination assays. These proof-of-concept experiments demonstrate that the catalytic domain of GIY-YIG homing endonucleases can be targeted to relevant loci by fusing the domain to characterize DNA-binding platforms. Recent efforts have focused on improving the Tev-TAL platform by (1) understanding the spacing requirements between the nuclease cleavage site and the DNA binding site, (2) probing the DNA binding requirements of the I-TevI linker domain, and (3) demonstrating activity in mammalian systems.
MatK DNA Sequences and Phylogenetic Reconstruction in Saxifragaceae s. str

Comparative DNA sequencing of matK, a maturase coding gene located within the intron of the chloroplast gene trnK, was evaluated for phylogenetic utility using genera of Saxi- fragaceae s. str. The entire matK gene was sequenced for two members of the family, Sullivantia sullivantii and Saxifraga integrifolia. Comparison of base substitution rates between these two species indicated that matK evolves approximately three-fold faster than rbcL. Comparative sequencing of 754 base pairs within matK was subsequently conducted using 25 genera in Saxifragaceae s. str. and two outgroup taxa. Summed over the 31 taxa sequenced for this 754 base pair region, 40% of the base positions were variable and 15.6% were potentially informative. Five insertion/deletion events of three or six base pairs were also detected. Skewness and randomization tests both suggest that significant non-random structure is present in the matK data set. Parsimony analyses provided 72 most parsimonious trees of 223 steps (excluding autapomorphies) with a consistency index of 0.565. Several well-supported groups of genera are highly concordant with relationships suggested by two other chloroplast DNA data sets: chloroplast DNA restriction sites and rbcL sequences.
Genetic diversity, taxonomy, and phylogeography of the western Palaearctic water strider Aquarius najas (DeGeer) (Heteroptera: Gerridae)

Genetic diversity and phylogeography was surveyed for the western Palaearctic water strider (Heteroptera, Gerridae) Aquarius najas (DeGeer) based on almost 780 bp of DNA sequence data from the mitochondrial gene encoding cytochrome oxidase c subunit I from 62 specimens sampled throughout its distribution area, and including the two related species A. cinereus (Puton) (6 specimens) and A. ventralis (Fieber) (3 specimens). 34 unique haplotypes were found for A. najas (55%), 5 for A. cinereus (83%), and 3 for A. ventralis (100%). A strict consensus tree obtained after a maximum parsimony analysis of the unique haplotypes was well resolved, and showed that while monophyly of A. ventralis was strongly supported, A. najas and A. cinereus were not reciprocally monophyletic in their mtDNA, since two clades from North Africa include a mixture of both species, and one specimen of A. cinereus from Sicily could not be assigned to any clade. All haplotypes were geographically well structured, and only one A. najas haplotype was widely distributed. This haplotype belongs to a clade with very little sequence divergence, which is distributed throughout Central and northern Europe, but includes also populations from northern Italy and northern Greece, thus indicating one or two possible refuges during the Pleistocene glaciations. Specimens from the Iberian Peninsula and Mallorca comprised a sistergroup to this clade, and show that the Iberian refugium did not contribute to the fauna of other parts of the European continent. Around the Mediterranean the sequence divergences for A. najas were much higher, especially in the Balkans, and on the islands Crete and Corsica. Whether the occurrence of these highly distinct lineages around the Mediterranean is due to extinction - or insufficient sampling - of intermediate haplotypes, or whether it indicates presence of unrecognized sibling species of A. najas and A. cinereus clearly needs further investigation.
Measuring an Interaction of Sexual Harassment and Employee Job Satisfaction

Sexual harassment is an ongoing issue which unlikely to be reported by the employees who experienced. The sexual provocation towards employees are likely to be happened at the workplace despite the gender is. Previous studies revealed that, women in automotive industry are more likely to expose and experience sexual harassment as the proportion of women are less in this industry. This paper aims to investigate sexual harassment relation with employee job satisfaction. Questionnaires were distributed to employee in automotive industry in Malaysia. A total of 204 usable questionnaires were analysed. The findings indicated that sexual harassment is partially significant with employee job satisfaction. The result could assist the company in drafting policies in an organization aiming at minimizing the occurrence of sexual harassment in work place environment.
MagnifierNet: Towards Semantic Adversary and Fusion for Person Re-identification

Although person re-identification (ReID) has achieved significant improvement recently by enforcing part alignment, it is still a challenging task when it comes to distinguishing visually similar identities or identifying the occluded person. In these scenarios, magnifying details in each part features and selectively fusing them together may provide a feasible solution. In this work, we propose MagnifierNet, a triple-branch network which accurately mines details from whole to parts. Firstly, the holistic salient features are encoded by a global branch. Secondly, to enhance detailed representation for each semantic region, the "Semantic Adversarial Branch" is designed to learn from dynamically generated semantic-occluded samples during training. Meanwhile, we introduce "Semantic Fusion Branch" to filter out irrelevant noises by selectively fusing semantic region information sequentially. To further improve feature diversity, we introduce a novel loss function "Semantic Diversity Loss" to remove redundant overlaps across learned semantic representations. State-of-the-art performance has been achieved on three benchmarks by large margins. Specifically, the mAP score is improved by 6% and 5% on the most challenging CUHK03-L and CUHK03-D benchmarks.
Bleaching of color of kraft pulp mill effluents and natural organic matter in lakes

In situ changes in the color of lake water and biologically treated kraft pulp mill effluents mixed in lake water were examined in three lakes. In 1.7 m 3 enclosures, the color (400-700 g Pt·m -3 ) of the mixture of pulp mill effluent and lake water remained similar for 4 months in darkness, whereas exposure to solar radiation resulted in 17- 42% bleaching of color. During the summer stratification of a humic lake, the color (160 g Pt·m -3 ) of hypolimnetic water remained similar to that found during spring turnover, but the color of epilimnion decreased 13%. The measured rates of photochemical bleaching of color by solar radiation could explain the decrease of epilimnetic color. Attenua - tion of photobleaching in the water column was similar to that of blue irradiance. Measured photobleaching was well described by a model based on quantum yields. The model revealed that visible solar radiation accounted for 29% of photobleaching at the surface and 66% in the whole water column. The results indicated that solar radiation, rather than plankton, bleached color of lake water and pulp mill effluents in surface strata.