Miracle-dz/UniFAR-FTUs huggingface dataset:
Contrasting the influences of stream inputs and landscape position on bacterioplankton community structure and dissolved organic matter composition in high‐elevation lake chains

We examined bacterioplankton community composition (BCC; using polymerase chain reaction‐based phylogenetic fingerprinting) and the composition of dissolved organic matter (DOM; using scanning fluorometry) within lake chains in high‐elevation catchments of the Sierra Nevada, California. We compared temporal BCC dynamics in two connected cirque lakes and their inlets and found that lake : inlet community similarity of both lakes declined with increasing residence time because of seasonal snowpack melting. However, for over 3 yr the headwater lake remained distinct from its inlet even during peak snowmelt (residence time <5 d), whereas the downstream lake was consistently similar to both its inlet and the upstream lake. We subsequently sampled 17 lake chains (4–8 lakes each) throughout the Sierra Nevada longitudinally to examine how landscape position and catchment characteristics influenced the similarity in BCC and DOM among lakes and their connecting streams. Headwater lakes were significantly less similar to their inlet streams than were downstream lakes. BCC was heterogeneous throughout each catchment, but headwater inlet BCC was consistently distinct from all downstream samples. In contrast, DOM exhibited a trend of increasing homogeneity as water moved downstream: upstream and downstream waters were distinct but without clear headwater outliers. The similarity in BCC and DOM between any two lakes within a chain declined with geographic distance. Our results illustrate the contrasting roles of environmental selection and dispersal in structuring bacterioplankton communities, supporting both strong within‐lake environmental selection for communities distinct from terrigenous inputs and a weaker effect of geographic dispersal differentiating communities among lakes linked by advective flow.
Structural Characterization of TssL from Acinetobacter baumannii: a Key Component of the Type VI Secretion System

Several Acinetobacter species carry one functional type VI secretion system (T6SS). The T6SS is encoded in a single locus containing 16 conserved genes, most of which code for proteins essential to T6SS activity. One of these key components is TssL, a cytoplasmic protein bound to the inner membrane. Despite its importance and its particular characteristics, the structure of T6SS in A. baumannii remains understudied. Here, we present structural, in silico, and in vivo studies of TssL, highlighting the importance of two well-conserved residues and improving our understanding of this secretion system in this bacterium. ABSTRACT The type VI secretion system (T6SS) is a complex molecular nanomachine used by Gram-negative bacteria to deliver diverse effectors into adjacent cells. A membrane complex (MC) anchors this transport system to the bacterial cell wall. One of the proteins forming the MC is TssL, a cytoplasmic protein bound to the inner membrane through a single transmembrane helix. Here, we report the structure of the cytoplasmic N-terminal region of TssL from Acinetobacter baumannii, a bacterium encoding in a single locus a secretion system that is a special case among other T6SSs. The protein structure, consisting of two antiparallel alpha-helical bundles connected by a short loop, reveals several interesting particularities compared with homologous proteins from other organisms. In addition, we demonstrate the structural significance of residues Asp98 and Glu99, which are strongly conserved among T6SS-encoding Gram-negative bacteria. Mutations in these two residues strongly impact protein dynamics, expression, and functionality. Our results improve our understanding of the T6SS of A. baumannii, which remains largely understudied compared with that of other pathogens. IMPORTANCE Several Acinetobacter species carry one functional type VI secretion system (T6SS). The T6SS is encoded in a single locus containing 16 conserved genes, most of which code for proteins essential to T6SS activity. One of these key components is TssL, a cytoplasmic protein bound to the inner membrane. Despite its importance and its particular characteristics, the structure of T6SS in A. baumannii remains understudied. Here, we present structural, in silico, and in vivo studies of TssL, highlighting the importance of two well-conserved residues and improving our understanding of this secretion system in this bacterium.
Determination of the Geographical Origin of Honeys by Multivariate Analysis of Gas Chromatographic Data on Their Free Amino Acid Content

SummarySeventeen free amino acids have been determined by gas chromatography in 45 samples of honey obtained from the UK, Australia, Argentina and Canada. The results were analysed statistically (using canonical variates analysis) in order to test the validity of the use of amino acid data for characterizing the geographical origin of honey. Good discrimination was shown between the samples from Australia, Argentina and Canada. Those from the UK were classified as a group, but they fell between the samples from Argentina and Canada, and could be wholly discriminated only on further analysis when the well separated Australian group was omitted. These preliminary results substantiate the claim that certain groups of honey samples from certain foreign countries can be distinguished from one another on the basis of free amino acids. The statistical analysis should be improved with larger samples, but owing to the proportion of data omitted as outliers, and to the problem of classifying honeys as of UK origin,.
A synthesis of gynandromorphy among wild bees (Hymenoptera: Apoidea), with an annotated description of several new cases

Abstract We here describe six new specimens of gynanders (i.e. specimens where male and female phenotypic characters occur on the same individual) from distinct families of wild bees (Hymenoptera, Apoidea). The newly described cases include Melitta haemorrhoidalis (Fabricius 1775) (Melittidae), Dasypoda hirtipes (Fabricius 1775) (Melittidae), Anthophora plumipes (Pallas 1772) (Apidae), Bombus monticola rondoui Vogt 1909 (Apidae), Bombus vestalis vestalis (Fourcroy 1785) (Apidae) and Bombus vestalis sorgonis (Strand 1917) (Apidae). Descriptions are accompanied by behavioural observations of gynanders under natural conditions before their capture for two cases. We also list 109 gynanders already described in bees and we interpret the newly described cases along these previous records. The putative origins of gynandromorphy are then discussed in the light of recent advances in the field of sex determination in Hymenoptera.
Are endogenous opioid mechanisms involved in the effects of aerobic exercise training on chronic low back pain?: a randomized controlled trial.

Aerobic exercise is believed to be an effective chronic low back pain (CLBP) intervention, although its mechanisms remain largely untested. This study evaluated whether endogenous opioid (EO) mechanisms contributed to the analgesic effects of an aerobic exercise intervention for CLBP. Individuals with CLBP were randomized to a 6-week, 18-session aerobic exercise intervention (n = 38) or usual activity control (n = 44). Before and after the intervention, participants underwent separate laboratory sessions to assess responses to evoked heat pain after receiving saline placebo or i.v. naloxone (opioid antagonist) in double-blinded, crossover fashion. Chronic pain intensity and interference were assessed before and after the intervention. EO analgesia was indexed by naloxone-placebo condition differences in evoked pain responses (blockade effects). Relative to controls, exercise participants reported significantly greater pre-post intervention decreases in chronic pain intensity and interference (p's < .04) and larger reductions in placebo condition evoked pain responsiveness (McGill Pain Questionnaire-Short Form [MPQ]-Total). At the group level, EO analgesia (MPQ-Total blockade effects) increased significantly pre-post intervention only among female exercisers (p = .03). Dose-response effects were suggested by a significant positive association in the exercise group between exercise intensity (based on meeting heart rate targets) and EO increases (MPQ-Present Pain Intensity; p = .04). Enhanced EO analgesia (MPQ-Total) was associated with significantly greater improvement in average chronic pain intensity (p = .009). Aerobic exercise training in the absence of other interventions appears effective for CLBP management. Aerobic exercise-related enhancements in endogenous pain inhibition, in part EO-related, likely contribute to these benefits.
Behavior of the vegetable crops section in three types of food retail stores in Campo Grande, Brazil

The behavior of the fresh fruits and vegetable produce section was evaluated, under the point of view of the consumer, in three types of food retail stores in Campo Grande, Brazilian southeast: a grocery store ("quitanda"), a supermarket, and an open-air market. A quantitative-descriptive survey was conducted with 120 individuals, responsible for purchasing fresh fruit and vegetable produce for their homes. To accomplish that, twelve variables were investigated and adapted from the parameters used in the SERVQUAL model, in which the attributes of the retail outlet are pointed out, such as store hygiene and cleanliness and manner by which produce is displayed; employee training traits, such as courtesy and helpfulness; and quality, price range, and variety of the produce for sale. The results reveal that shopping for grocery is done weekly; 80% of the shoppers interviewed do their shopping in supermarkets and 94% do theirs in open-air markets. The open-air market had the best results in the attributes for which they were evaluated when compared with the grocery store and the supermarket. The study also points out that the older the shopper the more often he/she does grocery shopping.
Socio-environmental conflicts between the refugee populations and their host communities: The case of Eritrean Refugees in North Western Tigray, Ethiopia

Abstract This article presents research on the socio environmental impact of refugees on their host communities. We assessed the challenges of refuge populations to their host community in north western Tigray, Ethiopia, where the Eritrean refugees are settled,. Primary data was produced from semi-structured questionnaires and a random sampling technique deploying a logistic regression model to describe the relationship between the socio-environmental changes of the host community. Results suggested 96% of the sample respondents confirmed changes of tree species and forest coverage on community farms was observed during the study period. Similarly, change in forest coverage and changes in tree species are strongly and positively associated with the existence of refugees in the study communities. The dramatic change observed on forest coverage and tree species has led to a progressive decline in natural resources. The study concluded that unplanned human population influxes, especially refugee inflows, affected host communities negatively and the socio-environmental situation has been significantly changed in the study areas. The study highlights the need for holistic intervention to ameliorate the negative impacts and to maintain the sustainable management of natural resources so as to improve the socio-environmental impact of refugees on host communities.
Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission

Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The aim of this study was to investigate the occurrence and diversity of AdVs in: (i) fecal samples of apes and monkeys from different African countries (Republic of Congo, Senegal, Djibouti and Algeria), (ii) stool of humans living near gorillas in the Republic of Congo, in order to explore the potential zoonotic risks. Samples were screened by real-time and standard PCRs, followed by the sequencing of the partial DNA polymerase gene in order to identify the AdV species. The prevalence was 3.3 folds higher in NHPs than in humans. More than 1/3 (35.8%) of the NHPs and 1/10 (10.5%) of the humans excreted AdVs in their feces. The positive rate was high in great apes (46%), with a maximum of 54.2% in chimpanzees (Pan troglodytes) and 35.9% in gorillas (Gorilla gorilla), followed by monkeys (25.6%), with 27.5% in Barbary macaques (Macaca sylvanus) and 23.1% in baboons (seven Papio papio and six Papio hamadryas). No green monkeys (Chlorocebus sabaeus) were found to be positive for AdVs. The AdVs detected in NHPs were members of Human mastadenovirus E (HAdV-E), HAdV-C or HAdV-B, and those in the humans belonged to HAdV-C or HAdV-D. HAdV-C members were detected in both gorillas and humans, with evidence of zoonotic transmission since phylogenetic analysis revealed that gorilla AdVs belonging to HAdV-C were genetically identical to strains detected in humans who had been living around gorillas, and, inversely, a HAdV-C member HAdV type was detected in gorillas. This confirms the gorilla-to-human transmission of adenovirus. which has been reported previously. In addition, HAdV-E members, the most often detected here, are widely distributed among NHP species regardless of their origin, i.e., HAdV-E members seem to lack host specificity. Virus isolation was successful from a human sample and the strain of the Mbo024 genome, of 35 kb, that was identified as belonging to HAdV-D, exhibited close identity to HAdV-D members for all genes. This study provides information on the AdVs that infect African NHPs and the human populations living nearby, with an evident zoonotic transmission. It is likely that AdVs crossed the species barrier between different NHP species (especially HAdV-E members), between NHPs and humans (especially HAdV-C), but also between humans, NHPs and other animal species.
Molecular cloning and characterization of two lincomycin‐resistance genes, ImrA and ImrB, from Streptomyces lincolnensls 78–11

Two different lincomycin‐resistance determinants (ImrA and ImrB) from Streptomyces lincolnensis 78–11 were cloned in Streptomyces lividans 66 TK23. The gene ImrA was localized on a 2.16 kb fragment, the determined nucleotide sequence of which encoded a single open reading frame 1446 bp long. Analysis of the deduced amino acid sequence suggested the presence of 12 membrane‐spanning domains and showed significant similarities to the methylenomycin‐resistance protein (Mmr) from Streptomyces coelicolor, the QacA protein from Staphylococcus aureus, and several tetracycline‐resistance proteins from both Gram‐positive and Gram‐negative bacteria, as well as to some sugar‐transport proteins from Escherichia coli. The ImrB gene was actively expressed from a 2.7 kb fragment. An open reading frame of 837 bp could be localized which encoded a protein that was significantly similar to 23S rRNA adenine(2058)‐N‐methyltransferases conferring macrolide—lincosamide—streptogramin resistance. LmrB also had putative rRNA methyltransferase activity since lincomycin resistance of ribosomes was induced in ImrB‐containing strains. Surprisingly, both enzymes, LmrA and LmrB, had a substrate specificity restricted to lincomycin and did not cause resistance to other lincosamides such as celesticetin and clindamycin, or to macrolides.
Modelling of the Low-Pressure Gas Injector Operation

Abstract In recent years, there has been a growing interest in alternative sources of power supply for internal combustion engines. Liquefied petroleum gas injection systems are among the most popular. It becomes necessary to know mathematical descriptions of the operation of individual components. The article presents a mathematical model that describes the operation of the low-pressure gas injector. Valtek plunger injector was chosen as the test object. The mathematical description includes three parts, i.e. electric, mechanical and pneumatic. The electrical part describes the generation of electromagnetic force by a circuit with a coil, in the mechanical equilibrium equation of forces acting on the plunger, and in the pneumatic part the air pressure on the plunger. The calculations were performed in the Matlab/Simulink environment, creating current waveforms, acting forces and plunger displacement. Correctness of mathematical description and determined in the course of opening and closing time calculations were related to the values declared by the manufacturer, showing differences below 3%. The presented mathematical model can be modified for other injector design solutions.
Predicting the Concreteness of German Words

Concreteness of words has been measured and used in psycholinguistics already for decades. Recently, it is also used in retrieval and NLP tasks. For English a number of well known datasets has been established with average values for perceived concreteness. We give an overview of available datasets for German, their correlation and evaluate prediction algorithms for concreteness of German words. We show that these algorithms achieve similar results as for English datasets. Moreover, we show for all datasets there are no significant differences between a prediction model based on a regression model using word embeddings as features and a prediction algorithm based on word similarity according to the same embeddings.
Fitness of the pestiferous small rodent Mastomys natalensis in an agroecosystem in Mayuge district, Lake Victoria Crescent, Uganda

Abstract A 2.5-year study was conducted to understand the fitness of Mastomys natalensis in an agroecosystem in relationship with environmental predictors. The study was conducted in Mayuge district, in the Lake Victoria Crescent zone in Eastern Uganda. Fitness was measured in terms of survival, maturation and capture probability and estimated using multi-event capture-recapture models. Survival rates were higher after high rainfall in the previous month and increased with increasing population density of the animals. Maturation rate, on the other hand, showed no significant association with any predictor variables, while capture probability was significantly associated with sex of the animals, with higher capture probability for males. The results demonstrate that the fitness of M. natalensis in an agroecosystem is dependent on rainfall, sex and current population density. The aforementioned results were associated with increasing vegetation which provides cover for animal nesting and abundant food for the animals during rainfall periods and thus increased survival, high mobility in males in search for mates thus exposing animals to high chances of being captured and increased prey saturation at high population density resulting in high animal survival. These results have important implications for the timing of management strategies, i.e. control efforts should be enforced during the rainfall seasons to prevent high population buildup in the succeeding seasons.
Study of Toxicity Assessment of Heavy Metals from Steel Slag and Its Asphalt Mixture

Steel slag has been used widely as an aggregate in road application, but it could pose a contamination risk for the environment due to considerable heavy metals (HMs). To explore the leaching behavior and contamination risk of HMs from steel slag and its asphalt mixture is of great significance. In this study, the physical-chemical features, batch leaching test and semi-dynamic test were conducted to determine the mobility capability and leaching characteristics of HMs. The results show that steel slag presents a low pollution risk in short-term leaching, whereas the cumulative release mass of Cd, Ni, As and Pb are more than or approach the limits, which indicates that steel slag exhibits environment impacts to a certain extent. Steel slag covered with asphalt binder results in As and Cu reduced by 3.64% and 4.83%. Diffusion is the main controlling mechanism of HMs in asphalt mixture and the mobility capability of most HMs were classed as “low mobility” (LI > 8). Asphalt stripping off can aggravate the release potential of HMs from asphalt mixture, but the pollution risk remains controllable.
Breast Abscesses Caused by Anaerobic Microorganisms: Clinical and Microbiological Characteristics

The objectives of this study were to report the antimicrobial susceptibility of 35 clinically significant anaerobic bacteria isolated from breast abscesses between March 2017 and February 2020 in a tertiary hospital in Granada (Spain) and to describe key clinical features of the patients. Species identification was performed mainly by MALDI-TOF MS. Antimicrobial susceptibility tests were carried out against benzylpenicillin, amoxicillin–clavulanic acid, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin–tazobactam using the gradient diffusion technique and European Committee on Antimicrobial Susceptibility Testing EUCAST breakpoints (except for moxifloxacin). The most frequent anaerobes were Finegoldia magna (31.4%; n = 11), Actinomyces spp. (17.1%; n = 6), Propionibacterium spp. (17.1%; n = 6), and Prevotella spp. (14.2%; n = 5). Imipenem, amoxicillin–clavulanic acid, and piperacillin–tazobactam were universally active against all genera tested. High overall resistance rates to clindamycin were observed, especially for Gram-positive anaerobic cocci (56.2%) and Gram-positive anaerobic bacilli (38.4%). High resistance rates to metronidazole were also observed for Gram-positive (76.9%) and Gram-negative anaerobic bacilli (50%). High resistance rates to moxifloxacin were found for Gram-negative anaerobic bacilli (50%) and Gram-positive anaerobic cocci (31.2%). No breast abscess cases of Bacteroides spp. were detected. Routine antimicrobial susceptibility testing for anaerobes in breast abscesses may contribute to allow empirical therapies to be selected in accordance with local data on resistant strains.
Do Tamil newspapers educate the public about suicide? Content analysis from a high suicide Union Territory in India

Background: Responsible media reporting of suicide is a key population-level suicide prevention strategy. Thus far, there has been no systematic analysis of media reporting of suicide in Puducherry, a consistently high suicide burden state in India. Aim: To evaluate the adherence of media reports of suicide against the World Health Organization (WHO) guidelines in Puducherry. Method: We conducted a year-round content analysis of all suicide-related reports in the two most widely read vernacular dailies of Puducherry. We used a pre-defined data extraction form and coded each item based on the WHO reporting guidelines. Results: A total of 318 suicide reports were retrieved. Harmful reporting practices such as mentioning the method of suicide (99.1%), description of the steps involved (68.2%) and location of suicide (86.5%), monocausal explanations (91.8%), and inciting life events (52.5%) were common. Helpful practices such as mentioning warning signs (1.3%), recognizing links with mental health disorders (3.8%) and effects on bereaved persons (2.2%) were rare. Only one article (0.31%) included any content related to educational/preventive aspects of suicide. Conclusion: Media reporting of suicide in Puducherry, India, does not adhere to reporting guidelines and there is very little focus on educating the public about preventive aspects of suicide. Urgent efforts are warranted to improve the quality of media reporting which should include the development of national guidelines on suicide reporting and collaborative efforts that take into account barriers and perspectives of media professionals.