Miracle-dz/UniFAR-FTUs huggingface dataset:
EFFECT OF SOME MICRONUTRIENTS FOLIAR APPLICATION ON CHEMICAL CHARACTERS OF TWO GROUNDNUT CULTIVARS

Two filed experiments were carried out in two successive summer seasons (2016 and 2017) at the Production and Research Station, National Research Centre, ElNubaria Province, El-Beheira Governorate, Egypt to investigate the response of two groundnut (Giza 6 and Gregory) cultivars to some micronutrients foliar application i.e. Zinc, Manganese and Boron and their interaction with different growth stages on some chemical traits of groundnut seeds (macro and micronutrients seed content (%), essential and non-essential amino acids composition, saturated and unsaturated fatty acids composition and saturated and unsaturated fatty acids ratios ) under reclaimed sandy soil condition. The data showed that groundnut cultivars were differed in macronutrients seed content (%) affected by micronutrient foliar spraying application treatments, Giza 6 surpassed Gregory cultivar in macronutrient seed content (N, P and K %), where the lowest values of macronutrients seed content % were observed by control treatments (without foliar application). The highest micronutrients seed content were obtained by the mixture of thrice micronutrient foliar application treatment (Zn + Mn + B) with Giza 6 and Gregory cultivars, which records 107.65 and 96.47, 50.65 and 55.0, 27.95 and 26.20, 1.60 and 1.83 (ppm) for Fe, Zn, Mn and B, respectively. Amino acids profile of seed protein indicated that Leucine and Phenylalanine acids were the major content of essential amino acids in the seed protein content. The interaction between groundnut cultivars and micronutrients foliar application on non-essential amino acids composition that, Giza 6 surpassed Gregory cultivar in most non-essential amino acid; aspartic, serine, glutamic, glycine, argnine, proline and cystine, while Gregory cultivar recorded highest value of alanine, histidine. Slightly differences were observed between groundnut cultivars and micronutrients foliar spraying treatments on unsaturated fatty acid composition. Gregory cultivar recorded the highest value of oleic/linoleic ratio (1.88) when treated with dual application of micronutrients foliar Zn + B. On the contrary, the least value of O/L ratio recorded 1.77 with Gregory cultivar when treated with control treatment. Keyword: Amino acid composition, Fatty acid, Groundnut, Micronutrients
A Local Graph-Based Structure for Processing Gigantic Aggregated 3D Point Clouds

We present an original workflow for structuring a point cloud generated from several scans. Our representation is based on a set of local graphs. Each graph is constructed from the depth map provided by each scan. The graphs are then connected together via the overlapping areas, and careful consideration of the redundant points in these regions leads to a piecewise and globally consistent structure for the underlying surface sampled by the point cloud. The proposed workflow allows structuring aggregated point clouds, scan after scan, whatever the number of acquisitions and the number of points per acquisition, even on computers with very limited memory capacities. To show that our structure can be highly relevant for the community, where the gigantic amount of data represents a real scientific challenge per se, we present an algorithm based on this structure capable of resampling billions of points on standard computers. This application is particularly attractive for simplifying and visualizing gigantic point clouds representing very large-scale scenes (buildings, urban scenes, historical sites.), which often require a prohibitive number of points to describe them accurately.
Exploring Effective Sensory Experience in the Environmental Design of Sustainable Cafés

The aim of this study was to explore and construct spatial indicators suitable for green café ambience. The indicators were further empirically verified. A three-round questionnaire survey, based on the Delphi method, was conducted with 15 experts, including university professors (food and beverage services management and interior environmental design), café operators, and personnel from government agencies. Data were collected, and the results on the characteristics of the repeated feedback from the experts were convergent. Thirty-six indicators suitable for the design of green café ambience were extracted, of which 17 were verified by actual cafés as highly operable. The five-sense indicators of sustainable green ambience design obtained in this study can facilitate positive customer experiences and enhance the appeal of maintaining sustainable green trends for cafés. These indicators can also provide references for café operators in business planning and green café ambience design.
Behavioural barriers and perceived trade-offs to care-seeking for tuberculosis in the Philippines

ABSTRACT Effective tuberculosis (TB) treatment has existed for more than 50 years, but TB remains a leading cause of death worldwide and in the Philippines, in part because symptomatic individuals delay or avoid seeking care. Through qualitative interviews in Pampanga, Philippines, we investigated barriers to care-seeking using a behavioural science lens. We found barriers to TB care-seeking to be shaped by: (1) ambiguous symptoms; (2) association of TB risk with lifestyle and habits; (3) expectations of stigma, discrimination, and isolation; (4) short-term costs and long-term financial burden of TB; and (5) visibility of care in public sector facilities. Findings suggest that these barriers are deeply intertwined and that, typically, it is a combination of barriers that holds back a particular symptomatic individual from seeking care, as the barriers influence implicit trade-offs related to health, social, and financial consequences of having TB or another serious illness and of seeking care or not seeking care. The findings suggest avenues for more effectively reaching those with symptoms and their family members to encourage care-seeking by elevating the perceived benefits and putting perceived costs in proper perspective.
An Examination of Subway Sex Offense Modus Operandi: A Case of Seoul, South Korea

Subway sex offenses are a serious issue in the nations around world, but existing research has failed to explore the offense types or patterns systematically. In order to fill this gap, the authors employed Crime Script Analysis (CSA) to examine the two most common subway sex offenses in Seoul, South Korea. Specifically, the authors assessed the reasoning behind the steps taken to prepare for, carry out, and complete harassment and surreptitious recording. The offenses committed in the subway stations around the city of Seoul were analyzed based on the interviews with the subway police, official crime reports, and crime case files. Drawing from the findings, theoretical and practical implications are discussed.
An inducible glycogen synthase-1 knockout halts but does not reverse Lafora disease progression in mice

Malstructured glycogen accumulates over time in Lafora disease (LD) and precipitates into Lafora bodies (LBs), leading to neurodegeneration and intractable fatal epilepsy. Constitutive reduction of glycogen synthase-1 (GYS1) activity prevents murine LD, but the effect of GYS1 reduction later in disease course is unknown. Our goal was to knock out Gys1 in laforin (Epm2a)-deficient LD mice after disease onset to determine whether LD can be halted in midcourse, or even reversed. We generated Epm2a-deficient LD mice with tamoxifen-inducible Cre-mediated Gys1 knockout. Tamoxifen was administered at 4 months and disease progression assessed at 12 months. We verified successful knockout at mRNA and protein levels using droplet digital PCR and Western blots. Glycogen determination and periodic acid–Schiff–diastase staining were used to analyze glycogen and LB accumulation. Immunohistochemistry using astrocytic (glial fibrillary acidic protein) and microglial (ionized calcium-binding adapter molecule 1) markers was performed to investigate neuroinflammation. In the disease-relevant organ, the brain, Gys1 mRNA levels were reduced by 85% and GYS1 protein depleted. Glycogen accumulation was halted at the 4-month level, while LB formation and neuroinflammation were significantly, though incompletely, prevented. Skeletal muscle analysis confirmed that Gys1 knockout inhibits glycogen and LB accumulation. However, tamoxifen-independent Cre recombination precluded determination of disease halting or reversal in this tissue. Our study shows that Gys1 knockdown is a powerful means to prevent LD progression, but this approach did not reduce brain glycogen or LBs to levels below those at the time of intervention. These data suggest that endogenous mechanisms to clear brain LBs are absent or, possibly, compromised in laforin-deficient murine LD.
Plexus-Specific Effect of Flicker-Light Stimulation on the Retinal Microvasculature Assessed with Optical Coherence Tomography Angiography.

In neural tissues, the coupling between neural activity and blood flow is a physiological key principle in blood flow regulation. We used optical coherence tomography angiography to investigate stimulus-evoked hemodynamic responses in different microvascular layers of the human retina. Twenty-two healthy subjects were included. Vessel density before and during light stimulation was measured using optical coherence tomography angiography and assessed for the superficial, intermediate and deep capillary plexus of the retinal circulation. Volumetric blood flow was measured using a custom-built Doppler optical coherence tomography system. Our results show that flicker stimulation induced a significant increase in the vessel density of +9.9±6.7% in the superficial capillary plexus, +6.6±1.7% in the intermediate capillary plexus and +4.9±2.3% in the deep capillary plexus. The hyperemic response of the superficial capillary plexus was significantly higher compared to the intermediate capillary plexus (p=0.02) and deep capillary plexus (p=0.002). Volumetric retinal blood flow increased by +39.9±34.9% in arteries and by +29.8±16.8% in veins. In conclusion, we showed a strong increase in the retinal microvascular density in response to light stimulation, with the most pronounced effect in the superficial capillary plexus. This is compatible with the hypothesis that the microvasculature exerts an important function in mediating functional hyperemia in humans.
Dammarenediol suppresses the growth, migration and invasion of human osteosarcoma cells by modulating PI3K/Akt and STAT-3 signaling pathways.

PURPOSE The present work was aimed at exploring the anticancer potential of dammarenediol against human osteosarcoma cells together with exploration of its mechanism of action. METHODS The osteosarcoma cell line HOS was used in this study. The proliferation rates of cancer cells were determined through CCK-8 kit. The colony forming assay was carried out to analyze the viability of osteosarcoma cells. The migration and invasion of osteosarcoma cells was examined by the transwell chamber method. Western blotting was used to elucidate the protein expression levels. RESULTS The osteosarcoma cell growth was inhibited by dammarenediol in a dose-dependent manner, while the normal bone cell line was not affected much. Dammarenediol further declined the viability of cancer cells along with restricting their metastatic potential. The anticancer effects of dammarenediol were attributed to the blockage of PI3K/Akt and STAT-3 signaling pathways. CONCLUSION Dammarenediol effectively restricted the growth and metastasis of osteosarcoma cells in vitro by inhibiting the PI3K/Akt and STAT-3 signaling pathways.
Transmembrane signaling and cytoplasmic signal conversion by dimeric transmembrane helix 2 and a linker domain of the DcuS sensor kinase

Transmembrane (TM) signaling is a key process of membrane-bound sensor kinases. The C4-dicarboxylate (fumarate) responsive sensor kinase DcuS of Escherichia coli is anchored by TM helices TM1 and TM2 in the membrane. Signal transmission across the membrane relies on the piston-type movement of the periplasmic part of TM2. To define the role of TM2 in TM signaling, we use oxidative Cys cross-linking to demonstrate that TM2 extends over the full distance of the membrane and forms a stable TM homodimer in both the inactive and fumarate-activated state of DcuS. An S186xxxGxxxG194 motif is required for the stability and function of the TM2 homodimer. The TM2 helix further extends on the periplasmic side into the α6-helix of the sensory PASP domain and on the cytoplasmic side into the α1-helix of PASC. PASC has to transmit the signal to the C-terminal kinase domain. A helical linker on the cytoplasmic side connecting TM2 with PASC contains an LxxxLxxxL sequence. The dimeric state of the linker was relieved during fumarate activation of DcuS, indicating structural rearrangements in the linker. Thus, DcuS contains a long α-helical structure reaching from the sensory PASP (α6) domain across the membrane to α1(PASC). Taken together, the results suggest piston-type TM signaling by the TM2 homodimer from PASP across the full TM region, whereas the fumarate-destabilized linker dimer converts the signal on the cytoplasmic side for PASC and kinase regulation.
The Impact of New Technology on the Healthcare Workforce

Dramatic improvements in information technology have the potential to transform healthcare delivery, and a key question is how such changes will affect the healthcare workforce of the future. In this brief, we present the state of knowledge of the effects of health information technology on the workforce. We first lay out the rapidly changing healthcare landscape due to the greater availability and use of information and communication technology (ICT) followed by a description of the evolution of employment, wages, and education across the wide variety of occupations in the healthcare sector since 1980. The healthcare sector has outperformed the rest of the economy and has proven resilient to the multiple downturns over the last four decades, although some groups have done much better than others. Next, we review the literature on the effects of ICT on productivity in terms of patient health outcomes and resource use, as well as the effects on healthcare expenditure. We find that there is evidence of a positive effect of ICT (e.g., especially electronic health records) on clinical productivity, but (i) it takes time for these positive effects to materialize; and (ii) there is much variation in the impact, with many organizations seeing no benefits. Looking at the drivers of adoption, we find that the role of workers is critical, especially physicians’ attitudes and skills. Privacy laws, fragmentation, and weak competition are also causes of slow adoption. There is very little quantitative work that investigates directly the impact of new technology on workers’ jobs, skills, and wages, but what there is suggests no substantial negative effects. Our own analysis finds no evidence of negative effects looking at aggregate data and hospital-level event studies. These findings are consistent with studies outside of healthcare, which stress the importance of complementary factors (such as management practices and skills) in determining the success of ICT investments. We conclude that management initiatives to increase the skills of workers will be required if the healthcare workforce and society more generally are to substantially benefit from the adoption of these powerful tools. Acknowledgments: We thank the MIT Task Force on the Work of the Future for financial support and comments on earlier drafts. We have benefited immensely from discussions with Catherine Tucker and Cason Schmit. Leila Agha, David Autor, and Tom Kochan have also given generous and detailed comments.
The Needle in the Haystack—Searching for Genetic and Epigenetic Differences in Monozygotic Twins Discordant for Tetralogy of Fallot

Congenital heart defects (CHDs) are the most common birth defect in human with an incidence of almost 1% of all live births. Most cases have a multifactorial origin with both genetics and the environment playing a role in its development and progression. Adding an epigenetic component to this aspect is exemplified by monozygotic twins which share the same genetic background but have a different disease status. As a result, the interplay between the genetic, epigenetic and the environmental conditions might contribute to the etiology and phenotype. To date, the underlying causes of the majority of CHDs remain poorly understood. In this study, we performed genome-wide high-throughput sequencing to examine the genetic, structural genomic and epigenetic differences of two identical twin pairs discordant for Tetralogy of Fallot (TOF), representing the most common cyanotic form of CHDs. Our results show the almost identical genetic and structural genomic identity of the twins. In contrast, several epigenetic alterations could be observed given by DNA methylation changes in regulatory regions of known cardiac-relevant genes. Overall, this study provides first insights into the impact of genetic and especially epigenetic factors underlying monozygotic twins discordant for CHD like TOF.
Gap junction internalization and processing in vivo: a 3D immuno-electron microscopy study

ABSTRACT Gap junctions have well-established roles in cell–cell communication by way of forming permeable intercellular channels. Less is understood about their internalization, which forms double membrane vesicles containing cytosol and membranes from another cell called connexosomes or annular gap junctions. Here, we systematically investigated the fate of connexosomes in intact ovarian follicles. High-pressure frozen, serial-sectioned tissue was immunogold labeled for connexin 43 (Cx43, also known as GJA1). Within a volume corresponding to ∼35 cells, every labeled structure was categorized and had its surface area measured. Measurements support the concept that multiple connexosomes form from larger invaginated gap junctions. Subsequently, the inner and outer membranes separate, Cx43 immunogenicity is lost from the outer membrane, and the inner membrane appears to undergo fission. One pathway for processing involves lysosomes, based on localization of cathepsin B to some processed connexosomes. In summary, this study demonstrates new technology for high-resolution analyses of gap junction processing. This article has an associated First Person interview with the first author of the paper. Summary: Serial-section electron microscopy combined with immunogold labeling shows that internalized gap junctions undergo complex processing of both inner and outer membranes.
Supercell Approach in Tight-Binding Calculation of Si and Ge Nanowire Bandstructures

精力乐队结构[100 ] 有各种各样的剖面图的面向的 Si 和 Ge 量 nanowires 被使用 sp (3 ) 计算 d (5 ) s * 有 supercellapproach 的紧密绑定的模型。 结果与第一个原则方法获得的那些相比(即，密度功能的理论，或 DFT ) 。 在在硅和 germaniumnanowires 之间的乐队结构的差别被分析，金者保留 indirect-bandgap 和 siliconnanowire，这被显示出[100 ] 当电线直径缩小时，方向成为 direct-bandgap。 它与可得到的试验性的数据比较被显示出紧密绑定的方法在在 mesoscopic nanowiredevices 预言与搬运人运输相关的乐队结构参数是足够的并且以计算费用比 DFT 方法远优异。
Pathological studies and therapeutic management of equine cutaneous neoplasms suspected of sarcoids

Cutaneous neoplasms affecting equines is very important condition. The sarcoid is a type of skin tumor that affects the equine family and equine sarcoids remain a frustrating and challenging entity for veterinary practitioners and research scientists alike. The present study was conducted on 6 clinical cases (5 horses and 1 mule) of cutaneous neoplasm suspected of sarcoids. The clinical cases were diagnosed on the basis of clinical and pathological examination of the lesions. The age of animals ranged from 1.5 years to 8 years. Grossly, the lesions were that of nodular, verrucous and occult form of sarcoid appearances which were present on nostrils, lips, neck, shoulder, back and face region. Gross and histopathological findings revealed 4 cases having fibroma like features characterized by whorls or interlacing bundles of fibrous connective tissue which run in all directions, one of squamous cell carcinoma characterized by neoplastic aggregates of pleomorphic epithelial cells with keratinized pearl formation and one of papilloma characterized by lesions of orthokeratotic hyperkeratosis, finger-like papillae with numerous vacuolating cells with eccentrically placed nuclei. The cases were treated by administrating anthiomaline (lithium antimony thiomalate) @ 15ml deep I/M on alternate days in adult animals and 7 ml deep intramuscularly on alternate days in young ones along with supportive therapy. Vincristine sulphate at the dose rate of 0.025/kg body weight I/V at weekly intervals for 2 to 4 times was incorporated in the treatment schedule if the cases do not respond to anthiomaline. The efficacy of anthiomaline in the treatment of sarcoid suspected cases was found to be 50% only and all were treated after vincristine administration. The results shows that anthiomaline along with vincristine sulphate is more effective as compared to anthiomaline alone in the treatment of equine sarcoid.
Lung-Protective Ventilation in the Emergency Department: A Quality-Improvement Study

Background: Pulmonary complications develop in more than 20% of emergency department (ED) patients receiving mechanical ventilation. Use of lung protective tidal volume (TV) can reduce adverse outcomes such as acute respiratory distress syndrome (ARDS). We sought to determine the impact of an educational intervention on awareness of and adherence to ARDS network (ARDSnet) guidelines for TV settings and clinical outcomes. Methods: This prospective observational study enrolled a convenience sample of patients > 18 years old requiring ED intubation and ventilation. ARDSnet-based educational interventions were provided. Awareness, defined as use of any predicted body weight (PBW) calculator and adherence, defined as use of correct PBW-based TV were compared pre-and post-intervention. Clinical outcome (development of ARDS within a 72-hour period) was determined. Results: Enrolled were 47 preand 30 post-intervention patients. Awareness improved from 34.8% pre-intervention to 69.0% postintervention (difference 34.2%; 95% CI 12.4 to 55.9%; p = 0.004). Adherence to ARDSnet guidelines did not improve (71.7% preintervention to 82.1% post-intervention [difference 95%; CI -8.9 to 29.7%; p= 0.31]). There was no difference in the presence of ARDS between groups at 72-hours (p=0.29). Conclusion: Awareness of ARDSnet guidelines improved with an educational intervention. Presence of ARDS between the preand post-intervention groups did not differ.