Miracle-dz/UniFAR-FTUs huggingface dataset:
Determination of blood glucose using 4-amino phenazone as oxygen acceptor.

Microtechniques are now used extensively in diagnostic as well as immunological studies for viruses, protozoa, In microtitrations constant volumes of fluids are picked up and delivered by specially constructed and calibrated metallic microdiluters. According to the Microtiter Instruction Manual of the Cooke Engineering Company (1965), their high titanium, stainless steel, new-pattern microdiluters with a precisely slotted tip are not supposed to rust, but in the hot and humid climate of Calcutta they do rust, though they continue to deliver fluids properly when tested on the special 'go-no-go' delivery tester supplied by the manufacturer. Many of the rust particles become detached from the microdiluters during their agitation in the wells of the dilution plates and produce, both with human group 0 and goose erythrocytes, an atypical settling pattern at the bottom of disposable plastic V plates. There is a small central button of erythrocytes, showing as well as the peripheral deposit, small black particles rimmed by a clear space. The peripheral concentric erythrocyte deposit closely simulated the typical haemagglutination pattern. Microscopically many black rust particles of various sizes and shapes were observed among discrete erythro-cytes, and we have called this atypical pattern pseudo-haemagglutination. The rusted particles did not alter the typical agglutina-tion pattern or the titre of some of the haemagglutinin-positive enteroviruses and arboviruses. They, however, produced pseudo-haemagglutination with the haemag-glutinin-negative enteroviruses as well as the uninoculated tissue culture maintenance medium. It appears that the rusted microdiluters, if used for diluting the antisera or the haemagglutinin-positive antigens, are not likely to modify the positive haemagglutination patterns. But one has to be cautious in using the rusted microdiluters for screening antigens as the detached rust may of itself produce a false-positive haemagglutination pattern. Although the dl adrenaline method has worked very well in this laboratory (Trinder, 1969), the manual version is a little insensitive and the colour development time is rather long. I have now worked out a method of using 4-amino phenazone which is three times as sensitive as the dl adrenaline method, uses only two relatively stable solutions , and requires only a 10-minute colour development. The two solutions required are: 1 PROTEIN PRECIPITANT Prepared exactly as for the dl adrenaline method but containing 01 % w/v phenol. Keeps indefinitely. 2 COLOUR REAGENT Fermcozyme 653 AM, 5 ml, and 5 ml of 0-1 % peroxidase (R.Z. 0-6) are added to 300 ml of a solution containing 1% w/v Na5HP04, 0-1% …
Paralleling Genetic Annealing Algorithm on Grid

Protein structure prediction (PSP) aims to search min energy for protein, which is a problem of non-deterministic polynomial (NP). A lot of algorithms have been proposed to solve this problem, and the time cost of the algorithms are significantly expensive due to complex computation. Genetic annealing algorithm (GAA) combining with simulated annealing algorithm and genetic algorithm, which is one of the most representative algorithms to be applied into the PSP, also requires large computing power for the complexity of the algorithm itself. To improve the efficiency of the algorithm, we established a grid system, implemented one parallel GAA algorithm in this system, and made some improvements to this algorithm. The results of the experiments shown that the parallel GAA's maximum speed-up rate is 3.81 times the serial algorithm.
Fundamental Escherichia coli biochemical pathways for biomass and energy production: creation of overall flux states

We have previously shown that the metabolism for most efficient cell growth can be realized by a combination of two types of elementary modes. One mode produces biomass while the second mode generates only energy. The identity of the four most efficient biomass and energy pathway pairs changes, depending on the degree of oxygen limitation. The identification of such pathway pairs for different growth conditions offers a pathway‐based explanation of maintenance energy generation. For a given growth rate, experimental aerobic glucose consumption rates can be used to estimate the contribution of each pathway type to the overall metabolic flux pattern. All metabolic fluxes are then completely determined by the stoichiometries of involved pathways defining all nutrient consumption and metabolite secretion rates. We present here equations that permit computation of network fluxes on the basis of unique pathways for the case of optimal, glucose‐limited Escherichia coli growth under varying levels of oxygen stress. Predicted glucose and oxygen uptake rates and some metabolite secretion rates are in remarkable agreement with experimental observations supporting the validity of the presented approach. The entire most efficient, steady‐state, metabolic rate structure is explicitly defined by the developed equations without need for additional computer simulations. The approach should be generally useful for analyzing and interpreting genomic data by predicting concise, pathway‐based metabolic rate structures. © 2004 Wiley Periodicals, Inc.
Model-Based Analysis and Understanding of Check Forms

Check forms are used by many people in daily life for money remittances. Surprisingly, the processing of these forms at banks and post offices is only partly automated. In this paper, we consider a particular kind of form, viz., the GIRO check forms used in Switzerland. We will describe a fully automatic system which is able to recognise the financial institution, the name and address of the receiver, and the account number on a GIRO check. The system comprises procedures for binarization, segmentation, model matching, and optical character recognition (OCR). Experiments on a sample set of 48 checks have shown promising results in terms of both computation time and recognition accuracy.
Delta interconnected hybrid three-leg converters

This paper proposes possibilities of interconnected hybrid three-leg converters, that are comprise of combinations of two-level and/or three-level legs. In this way, four topologies are presented and compared in aspects such as harmonic distortion of grid currents, semiconductor losses and rating of power switches. The hybrid solution allows improvements in quality of the output converter voltages, reducing the harmonic distortion of grid current. This advantage can be turned into switching frequency reduction and consequently semiconductor losses reduction. Circuit model equations, simulation and experimental results (acting as shunt compensator and inverter) are presented as well.
Gender difference in the association between adult attention deficit hyperactivity disorder symptoms and morningness–eveningness

We aimed to investigate the association between attention deficit hyperactivity disorder (ADHD) and morningness–eveningness in adulthood. Subjects without psychiatric comorbidity on the Structured Clinical Interview for DSM‐IV Axis I Disorders (n = 344) completed the Morningness–Eveningness Questionnaire (MEQ) and the Adult Self‐Report Scale for ADHD. MEQ showed an independent and negative association with ADHD symptoms (P < 0.0001). In male subjects, both inattention (P < 0.0001) and hyperactivity–impulsivity were associated with MEQ (P = 0.01). In female subjects, only inattention was associated with MEQ (P < 0.001). Our findings suggest that eveningness may be strongly associated with inattention of adult ADHD and that it may be associated with hyperactivity–impulsivity of adult ADHD in male subjects only.
Gene expression profiling indicate role of ER stress in miR-23a~27a~24-2 cluster induced apoptosis in HEK293T cells

Previously, we had reported that overexpression of miR-23a~27a~24-2 cluster induces caspase-dependent and -independent apoptosis via JNK in human embryonic kidney (HEK293T) cells. Herein, we describe the molecular mechanism(s) responsible for miR-23a~27a~24-2 cluster induced apoptosis. Gene expression profiling was used to characterize the transcriptional response to miR-23a~27a~24-2 cluster overexpression in HEK293T cells. The microarray analysis gave 1,025 differentially expressed genes and analysis of the gene expression data with Ingenuity Pathway Analysis (IPA) software revealed p53 signaling, oxidative stress response and mitochondrial dysfunction among the top processes being affected. This data substantiates our previous study where we had reported increase of ROS and the release of proapoptotic factors such as cytochrome c (cyt c) and apoptosis-inducing factor (AIF) from the mitochondria to cytosol. Additionally, components of ER stress-mediated apoptosis pathway i.e., C/EBP homologous protein (CHOP/DDIT3/GADD153) and TRIB3, an Akt inhibitor were found to be significantly enriched. Also, the enhanced expression of ATF3 and ATF4 was observed at RNA as well as protein level in miR-23a~27a~24-2 cluster overexpressed HEK293T cells. Induction of BIM appeared to be specific, because ER stress caused only a minor change in the expression of the related BH3-only proteins BID or PUMA. The fact that miR-23a~27a~24-2 cluster triggered endoplasmic reticulum stress (ER stress) was further established by the increase in cytosolic calcium levels after overexpression of this cluster in HEK293T cells. These findings were also supported by PANTHER analysis wherein biological process categories of apoptosis and stress response were enriched. Taken together, this work underlines the role of ER stress in miR-23a~27a~24-2 cluster mediated apoptosis in HEK293T cells. Since the detailed knowledge of this cluster induced apoptosis has now been elucidated, the in vivo study of this cluster would help evaluate the prospect of its use as a therapeutic in diseases known to occur because of deregulation of apoptosis.
Optimal control for semi-active suspension with inerter

The benefits of the inerter in passive suspension have been well demonstrated. To investigate suspension performances with the inerter in semi-active suspension, eight well studied passive suspension configurations with a parallel connection to a variable shock absorber are analyzed in this paper. By applying the optimal control theory, an optimal solution for each configuration is obtained and numerically solved by the forward/backward sweep method. The result shows that under the considered performance measure, the use of inerter can improve ride comfort in general, where the effect can even be significant for some specific configurations, but has no obvious advantage in road holding and suspension travel performance compared with the conventional semi-active suspension.
SVM classification based on supervised subset density clustering

A way of combining SVM(Support Vector Machine) with Supervised Subset Density Clustering is proposed in this paper. How to minimize the training set of SVM by means of clustering is researched. Original center positions are of great importance to clustering accuracy. However the traditional clustering center choosing algorithm doesn't work properly when the same kind of samples aren't closely-spaced or the shape of the sample distribution isn't regular, an self-adaptive multiple centers choosing method is proposed to solve the problem. Another problem addressed in the paper is that there are areas that are covered by multi-class samples which is of great difficulty for traditional clustering to deal with, so a supervised method for the improved density clustering is designed to make out such areas and referring the samples to SVM. The experimental results show that the algorithm reduces the iteration time of the whole training process without compromising the accuracy and generalization capacity of the algorithm obviously.
Porównanie algorytmów liniowej regulacji predykcyjnej

Referat zawiera porownanie algorytmow predykcyjnych stosowanych w uk adach regulacji temperatury systemu ogrzewania budynku. Badania wykonano na modelu fizycznym umieszczonym w komorze klimatycznej. Porownanie dotyczy algorytmu DMC z modelem w postaci odpowiedzi skokowej, algorytmu GPC z modelem transmitancyjnym oraz algorytmu MPCS w dwoch wersjach: z obserwatorem stanu zbudowanym w oparciu o regu Ackermanna oraz z filtrem Kalmana z zastosowaniem funkcji ortonormalnych.
Degradation dynamics and residue analysis of flubendiamide in cabbage and soil by liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction.

To formulate a scientific basis for a reasonable spray dose and safe interval period of 20% flubendiamide water dispersible granule (WDG) on controlling vegetable pests, degradation dynamics of flubendiamide in cabbage and soil was analyzed in this study. Dissipation and residue of flubendiamide in 20% flubendiamide WDG in cabbage and soil under field conditions were investigated by liquid chromatography-tandem mass spectrometry with dispersive solid phase extraction. Results showed that the degradation dynamic equations of flubendiamide in cabbage and soil were based on the first-order reaction dynamic equations. The half-lives of the degradation of flubendiamide were 3.51 d to 3.96 d and 3.43 d to 3.87 d in the cabbage of Yangzhou and Jingzhou, respectively, and 4.42 d to 5.13 d and 4.37 d to 4.99 d in the soil of Yangzhou and Jingzhou, respectively. The terminal residues of flubendiamide in the cabbage of Yangzhou and Jingzhou were 0.0247 mg&middotkg-1 to 0.0393 mg&middotkg-1 and 0.0225 mg&middotkg-1 to 0.0273 mg&middotkg-1, respectively, when 20% flubendiamide WDG was applied at a dose of0.050g&middotm-2. Flubendiamide is safe to be applied in cabbage fields at the recommended dose.
Optimizing Interval Training at Power Output Associated With Peak Oxygen Uptake in Well-Trained Cyclists

Abstract Rønnestad, BR and Hansen, J. Optimizing interval training at power output associated with peak oxygen uptake in well-trained cyclists. J Strength Cond Res 30(4): 999–1006, 2016—The purpose of this study was to investigate the acute physiological responses of interval protocols using the minimal power output (MAP) that elicits peak oxygen uptake (V[Combining Dot Above]O2peak) as exercise intensity and different durations of work intervals during intermittent cycling. In randomized order, 13 well-trained male cyclists (V[Combining Dot Above]O2peak = 67 ± 6 ml·kg−1·min−1) performed 3 different interval protocols to exhaustion. Time to exhaustion and time ≥ 90% of V[Combining Dot Above]O2peak were measured with MAP as exercise intensity, and work duration of the intervals equals either 80% of T max, 50% of T max, or 30 seconds with recovery period being 50% of the work duration at intensity equal to 50% of MAP. The major findings were that the interval protocol using 30-second work periods induced longer time ≥90% of V[Combining Dot Above]O2peak and longer work duration at MAP intensity than the interval protocols using work periods of 50% of T max or 80% of T max (p ⩽ 0.05). There was no difference between the protocols using work periods of 50% of T max or 80% of T max. In conclusion, the present study suggests that the 30-second work interval protocol acutely induces a larger exercise stimulus in well-trained cyclists than the protocols using work periods of 50% of T max or 80% of T max. The practical application of the present findings is that fixed 30-second work intervals can be used to optimize training time at MAP and time ≥90% of V[Combining Dot Above]O2peak in well-trained cyclists using MAP exercise intensity and a 2:1 work:recovery ratio.
Restriction fragment length polymorphism analysis of the C1‐inhibitor gene in hereditary C1‐inhibitor deficiency

Four out of 12 kindreds with Type I hereditary angio‐oedema (HAE) were shown to have unique disease‐related restriction fragment length polymorphism (RFLPs) in one allele of the C1‐inhibitor gene. These RFLPs were used to localise the gene mutations responsible for them in each family. The four mutations affected exon 4, exon 6, exon 7 and exon 8, respectively. Mutations in exon 6 and exon 8 have not been described previously in Type I HAE. The other two mutations which comprised an exon 4 deletion and an exon 7 deletion have already been documented by other investigators. In each family the mutation was seen to cosegregate with the disease. Detection of a disease‐related RFLP in 30% of the Type I HAE kindred tested is higher than other published studies, and reflects the larger number of restriction enzymes employed. These results suggest that Type I HAE is likely to be associated with a multiplicity of gene mutations as is seen in other genetic diseases. A new C1‐inhibitor gene‐related RFLP in the normal population was also characterised. This may be useful as an indirect marker of the mutant C1‐inhibitor allele in certain families with Type I HAE.
The B-SMART Appropriate Medication-Use Process: A Guide for Clinicians to Help Patients-Part 2: Adherence, Relationships, and Triage.

“Increasing the effectiveness of adherence interventions is likely to have a far greater impact on health than any improvement in medical treatments, including highly promising advances in biomedical technology. ”13 By identifying barriers that prevent patients from taking their medications as prescribed, by providing patients with targeted solutions to the identified barriers, by motivating and empowering patients, by providing tools to keep patients on track, and by referring patients to other services when needed to address other needs, we will significantly improve adherence to therapy plans and improve overall outcomes more than any one component alone. Part 2 completes this B-SMART approach to medication optimization, by discussing: adherence tools and reminders to keep patients on track; relationships: building positive relationships; and triage: direct patients’ medication management plan into the broader health care system.
No evidence for larger brains in cooperatively breeding cichlid fishes

The social brain hypothesis posits that frequent social interactions, characteristic of group living species, select for greater socio-cognitive abilities and the requisite neural machinery. An extension of the social brains hypothesis, known as the cooperative breeding brain hypothesis, postulates that cooperatively breeding species, which live in stable social groups and provide allocare, face particularly pronounced cognitive demands because they must recognize, remember, and differentially respond to multiple group members. These socio-cognitive challenges are thought to have selected for increased cognitive capacity, supported by a bigger brain. To test the prediction that cooperative breeders have larger brains, we performed a phylogenetically controlled comparison of the whole brain masses of adult fish from 16 closely related species of cooperatively and independently breeding lamprologine cichlid species from Lake Tanganyika. We collected data on brain mass from males of eight species of lamprolo.
Two‐dimensional electrophoresis of basic proteins with equilibrium isoelectric focusing in carrier ampholyte‐pH gradients

A modified procedure for the two‐dimensional electrophoretic analysis of basic polypeptides is described. This method uses isoelectric focusing with carrier ampholytes in the first dimension, and sodium dodecyl sulfate‐electrophoresis in the second dimension. Counteraction of the cathodic drift is achieved by glass tube treatment (silanization), electrolyte modification (use of weak bases and acids), protection of the catholyte from carbon dioxide, and the addition of glycerol to the gel mix. Better resolution and reproducibility are obtained than with nonequilibrium pH gradient electrophoresis, since quasi equilibrium focusing can be obtained.
Defensive applications of gene transfer technology in the face of bioterrorism: DNA-based vaccines and immune targeting

Gene transfer involves the introduction of an engineered gene into a person’s cells with the expectation that the protein expressed from the gene will produce a therapeutic benefit. Strategies based on this principle have led to the approval of > 600 clinical trials and enrolment of ∼ 3500 subjects worldwide in attempts to treat diseases ranging from cancer to AIDS to cystic fibrosis. While gene therapy has met with limited success and still has many hurdles to overcome before it sees wide application, it may be useful as a defensive strategy against bioterrorism agents including infectious microbes and toxins. Although many defensive strategies are possible, immunological strategies are currently the most developed and are being actively applied to the development of strategies against several of the most virulent potential bioweapons. While most of these strategies are not yet ready for human application, DNA-based vaccines appear to be among the most promising in the fight against bioterrorism.
Effect of colchicine and phalloidin on the distribution of three plasma membrane antigens in rat hepatocytes: Comparison with bile duct ligation

The hepatocyte plasma membrane presents a morphological and functional regionalization into three domains: the sinusoidal; the lateral, and the canalicular. The mechanisms responsible for the biogenesis and maintenance of this regionalization are poorly understood. In this work, we have used colchicine and phalloidin, two drugs known to interfere with the secretory processes in hepatocytes, to study whether they also affect the transport of membrane proteins. The localization of three plasma membrane antigens was studied by light and electron microscopy using monoclonal antibodies identifying either the sinusoidal (A39) or the lateral (B1) or the canalicular (B10) domains in normal hepatocytes. In rats injected with colchicine (0.25 mg per 100 gm), A39 moved from the sinusoidal membrane to the lateral and canalicular ones, whereas B10 was displaced from the canalicular to the sinusoidal and lateral membranes, resulting after 8 hr in an almost equal labeling of the three domains with both antibodies. In rats injected daily for 7 days with phalloidin (50 μg per 100 gm), A 39 became mainly localized on the bile canalicular membrane instead of the sinusoidal one; B10 predominated on the canalicular membrane as in controls but in places it labeled the sinusoidal and lateral domains as well. In bile duct‐ligated rats studied for comparison for 4, 10 or 21 days, A39 and B10 localizations evolved as after phalloidin, but the changes were more marked. B1 was not affected by any of the treatments. In conclusion, colchicine, phalloidin and bile duct ligation do not seem to hinder the antigens in reaching the plasma membrane, but induce a redistribution of two of them, suggesting a disturbance in the biogenesis and/or control of the plasma membrane regionalization. Such an abnormal distribution could be involved in—or contribute to—the initiation of cholestasis.
Efficient physical optics approximation for the calculation of radiation patterns of planar antennas located on a finite ground plane

A physical optics (PO) model is applied to investigate the radiation characteristics of a microstrip patch antenna located on a finite ground plane. The standard PO approach consists of the replacement of the dielectric and the ground plane by electric volume and surface polarization currents. In this paper these polarization currents are obtained as a combination of polarization currents of the infinite structure and auxiliary polarization currents, only present beyond a certain distance from the source. Simple expressions are derived for these auxiliary currents, based on the expansion wave concept. The radiation patterns consist of two terms: the radiation pattern of the antenna on the infinite ground plane and a diffraction term, which takes into consideration the finiteness of the layer structure. The diffraction term is expressed in terms of integrals containing the auxiliary polarization currents. Some of these integrals can be evaluated analytically. Numerical and experimental results are presented.