Miracle-dz/UniFAR-FTUs huggingface dataset:
REGULAÇÃO DO CITOESQUELETO NAS CÉLULAS DO CANCRO DA BEXIGA APÓS TRATAMENTO FOTODINÂMICO CYTOSKELETON REGULATION IN BLADDER CANCER CELLS AFTER PHOTODYNAMIC TREATMENT

Photodynamic therapy (PDT) is a promising modality for the treatment of cancer that involves light of an appropriate wavelength and a photosensitizing drug (photosensitizer, PS), used in conjunction with molecular oxygen, leading to the production of reactive oxygen species (ROS). In a biological environment, these toxic species can interact with the cellular constituents eliciting cell death. Currently, the PS available show poor tumor specificity. Previous work from our research group reported a porphyrin conjugated with dendritic units of galactose (PorGal8) as a new water soluble PS, able to generate ROS after photoactivation and exhibiting increased selectivity to bladder cancer cells overexpressing galectin-1. Several studies reported cytoskeleton alterations derived from photodynamic treatments. However, the role of cytoskeleton disorganization in cell death induced by PDT remains unclear. In this work we evaluated whether changes in the cytoskeletal constituents actin filaments, intermediate filaments and microtubules are correlated with cell death triggered by PDT with PorGal8. The uptake of PorGal8 in two bladder cancer lines derived from transitional cell carcinoma (UM-UC-3 and HT-1376 cells), was concentration dependent. Cellular uptake of PorGal8 was higher in UM-UC-3 cells that express higher levels of galectin-1 protein than HT-1376 cells. PorGal8 was nontoxic in dark. Photoactivation of PorGal8 resulted in a significantly higher phototoxicity in UMUC-3 cells than HT-1376 cells. PorGal8 did not change the α-tubulin protein levels in UM-UC-3 cells but reduced α-tubulin twenty-four hours after photodynamic activation in HT-1376 cells. Although a few cells showed a recovery in microtubules organization, the fluorescence intensity decreased noticeably in most of the HT-1376 cells. A significant decrease in intermediate filaments (vimentin) protein levels was exhibited in both cell lines twenty-hours after irradiation. Thirty minutes post-irradiation, UM-UC-3 and HT-1376 cells showed a clear retraction of actin filaments with loss of stress fibers. Although no recovery was observed in UM-UC-3 cells, some cells present some reorganization in actin filaments, presenting short stress fibers, long extensions, like large filopodia, suggesting a possible recovery in HT-1376 cells. A small GTPases family protein, RhoA, referred to be involved with galectin-1 expression, was also evaluated, with preliminary results indicating a tendency towards an increase in HT-1376 cells twenty-four hours after therapy. Overall, our results give new insights into the mechanisms underlying the phototoxic effects of PorGal8. Better understanding the intrinsic web of events and alterations on cytoskeleton structures induced immediately after photodynamic treatment in resistant cancers may contribute to envisage new potential therapeutic adjuvants for PDT.
Cohesin complex-associated holoprosencephaly

Marked by incomplete division of the embryonic forebrain, holoprosencephaly is one of the most common human developmental disorders. Despite decades of phenotype-driven research, 80–90% of aneuploidy-negative holoprosencephaly individuals with a probable genetic aetiology do not have a genetic diagnosis. Here we report holoprosencephaly associated with variants in the two X-linked cohesin complex genes, STAG2 and SMC1A , with loss-of-function variants in 10 individuals and a missense variant in one. Additionally, we report four individuals with variants in the cohesin complex genes that are not X-linked, SMC3 and RAD21. Using whole mount in situ hybridization, we show that STAG2 and SMC1A are expressed in the prosencephalic neural folds during primary neurulation in the mouse, consistent with forebrain morphogenesis and holoprosencephaly pathogenesis. Finally, we found that shRNA knockdown of STAG2 and SMC1A causes aberrant expression of HPE-associated genes ZIC2 , GLI2 , SMAD3 and FGFR1 in human neural stem cells. These ﬁndings show the cohesin complex as an important regulator of median forebrain development and X-linked inheritance patterns in holoprosencephaly.
Stock Value Prediction System

:The use of artificial neural network is gaining popularity in the research field. Neural network consist of interconnected neurons which deciphers value by using input data by feeding network values. The main aim of our project is to use backpropagation process to predict the future value.Stock market prediction models are the most challenging fields in computer science. The aim of this project is implementation of neural networks with back propagation algorithm for stock value prediction . A neural network is a powerful data-modeling tool that is able to capture and represent complex input/output relationships. We apply Data mining technology to the stock in order to research the trend of the market. Our proposed system provides methods to develop machine learning stock market predictor based on Neural Networks using Back propagationalgorithm, with intent of improving the accuracy. In this paper we have used data mining process along with artificial neural network networking to predict the future value of the stock. This paper overcomes the all traditional statistical methods of the stock market value prediction.
Tritium In-Bed Accountability for a Passively Cooled, Electrically Heated Hydride (PACE) Bed

Abstract A PAssively Cooled, Electrically heated hydride (PACE) Bed has been deployed into tritium service in the Savannah River Site (SRS) Tritium Facilities. The bed design, absorption and desorption performance, and cold (non-radioactive) in-bed accountability (IBA) results have been reported previously. Six PACE Beds were fitted with instrumentation to perform the steady-state, flowing gas calorimetric inventory method. An IBA inventory calibration curve, flowing gas temperature rise (ΔT) versus simulated or actual tritium loading, was generated for each bed. Results for non-radioactive (“cold”) tests using the internal electric heaters and tritium calibration results are presented. Changes in vacuum jacket pressure significantly impact measured IBA ΔT values. Higher jacket pressures produce lower IBA ΔT values which underestimate bed tritium inventories. The exhaust pressure of the IBA gas flow through the bed’s U-tube has little influence on measured IBA ΔT values, but larger gas flows reduce the time to reach steady-state conditions and produce smaller tritium measurement uncertainties.
Anti-In ﬂ ammatory Potential of Green Synthesized Silver Nanoparticles of the Soft Coral Nephthea Sp. Supported by Metabolomics Analysis and Docking Studies

Background: Soft corals have been endorsed as a plentiful source of bioactive compounds with promising anti-in ﬂ ammatory activities; therefore, exploring their potential as source of anti-in ﬂ ammatory metabolites has stimulated a growing research interest. Purpose: To investigate the anti-in ﬂ ammatory potential of the soft coral, Nephthea sp. , in its bulk and silver nanostructure. Metabolomics analysis of Nephthea sp. , followed by molecular docking studies, was also conducted in order to explore and predict the secondary metabolites that might provide its inhibitory actions on in ﬂ ammation. Materials and Methods: The petroleum ether and ethyl acetate fractions were used to synthesize silver nanoparticles. The prepared silver nanoparticles were characterized through UV-vis spectrophotometric, transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR) analyses. Testing for the anti-in ﬂ ammatory activity was performed against COX-1 and COX-2. Furthermore, liquid chromatography – mass spectrometry (LC – MS) based metabolomics analysis and molecular docking were also applied. Results: A variety of secondary metabolites were identi ﬁ ed, among them, sesquiterpenes were found to prevail. The petroleum ether and acetone fractions of Nephthea sp. showed the highest COX-2 inhibitory activities, possibly attributable to their substantial contents of terpenoids. Additionally, the green synthesized silver nanoparticles of both the petroleum ether and ethyl acetate fractions of Nephthea sp. demonstrated higher anti-COX-2 properties. Conclusion: The obtained results showed the effectiveness of non-targeted metabolomics technique in metabolic pro ﬁ ling of Nephthea sp. , helping the search for new bioactive metabolites in future chemical studies on this soft coral. The interesting anti-in ﬂ ammatory potential of the tested extracts and their nanoparticles could also be relevant to the development of new, effective anti-in ﬂ ammatory agents.
CCID_A_312208 575..580

Department of Dermatology, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland Background: Localized scleroderma (LoS) is an inflammatory fibrosing disease of the connective tissue, whose esthetic sequelae are atrophic skin lesions with hyperpigmentation. The key element of diagnostic and therapeutic procedures is the assessment of the disease's severity and damage. The study objective was to analyze the usefulness of narrow-band reflectance spectrophotometry (NBRS) to assess erythema and hyperpigmentation in LoS lesions. Materials and Methods: Erythema indexes (EI) and melanin indexes (MI) were determined with the use of DermaLab Combo skin colour probe for LoS lesions and symmetrically located areas of normal skin. Then, relative percentage differences were determined for EI and MI, which were compared with the visual assessments of erythema and hyperpigmentation according to the Localized Scleroderma Cutaneous Assessment Tool (LoSCAT). Results: A total of 84 LoS lesions were studied in 41 patients. The study showed a moderate correlations between the spectrophotometric measurements and clinical assessments of erythema as well as hyperpigmentation (Spearman correlation coefficient, r), r = 0.37; p = 0.00047 and r = 0.55; p=0.0000001, respectively. Conclusion: NBRS seems to be a useful tool to assess the severity of erythema and hyperpigmentation in LoS lesions. Further studies are required in order to compare the spectrophotometric results with other objective methods.
OPTH_A_264912 4579..4586

1Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA; 2Alcon Research LLC, Fort Worth, TX, USA Purpose: To predict clinical defocus curve performance of the PanOptix intraocular lens (IOL) model TFNT00, a population-based image quality metric was applied to a pseudophakic eye model. Methods: Visual acuity (VA) was simulated using a 2-surface reduced eye model. For each virtual eye, the derived corneal surface was combined with scaled IOL surface. Corneal power and aberration, anterior chamber depth, and pupil size were iterated using a Monte-Carlo approach. Image quality of the IOLs was assessed using the total aberration map to compute the amplitude point spread function. A diffraction-normalized light-in-the-bucket metric was calculated for each virtual eye for defocuses from −3.5 D to +1.0 D (step size 0.25 D) and transformed to VAs and defocus curves. Simulated VA for the ReSTOR +3.0 D lens was used to generate a calibration function by linear regression correlation of simulated data with clinical VA data. Simulated TFNT00 VA was then validated by comparing defocus curves to clinical TFNT00 data. Results: From −3.5 D to +1.0 D, the simulated defocus curve was generally consistent with the defocus curve from the TFNT00 clinical trial. The mean absolute difference was 0.022 logMAR (~1 letter) for simulated VA versus clinical trial VA. Conclusion: IOL image quality can be assessed using a population-based virtual eye model to simulate VA and predict clinical performance. Computational modeling and simulation can be applied to future IOL development before clinical trials are conducted.
Efficient, high-density, carbon-based spinterfaces

The research field of spintronics has sought, over the past 25 years and through several materials science tracks, a source of highly spin-polarized current at room temperature. Organic spinterfaces, which consist in an interface between a ferromagnetic metal and a molecule, represent the most promising track as demonstrated for a handful of interface candidates. How general is this effect? We deploy topographical and spectroscopic techniques to show that a strongly spin-polarized interface arises already between ferromagnetic cobalt and mere carbon atoms. Scanning tunneling microscopy and spectroscopy show how a dense semiconducting carbon film with a low band gap of about 0.4 eV is formed atop the metallic interface. Spin-resolved photoemission spectroscopy reveals a high degree of spin polarization at room temperature of carbon-induced interface states at the Fermi energy. From both our previous study of cobalt/phthalocyanine spinterfaces and present x-ray photoemission spectroscopy studies of the cobalt/carbon interface, we infer that these highly spin-polarized interface states arise mainly from sp2-bonded carbon atoms. We thus demonstrate the molecule-agnostic, generic nature of the spinterface formation.
Research Online Research Online Perceived Parenting Styles and Values Development: A Longitudinal Study Perceived Parenting Styles and Values Development: A Longitudinal Study of Adolescents and Emerging Adults of Adolescents and Emerging Adults

Abstract © 2019 Society for Research on Adolescence This study explored parenting styles and values internalization. Perceived parenting styles were measured in Grade 7 (n = 749) and Grade 12 (n = 468), and values were measured in Grade 12 (n = 271) and one year postschool (n = 291). We measured three aspects of valuing: priority (extrinsic, intrinsic importance); regulation (controlled, autonomous); and successful enactment of values (success). Mothers’ authoritative parenting in Grade 7 predicted increased importance and autonomous regulation of values one year postschool. Fathers’ authoritative parenting in Grade 7 predicted decreased importance of extrinsic values. Fathers’ permissive parenting in Grade 7 predicted decreased importance of intrinsic values. Authoritarian parenting in Grade 12 predicted more controlled values regulation postschool, particularly for extrinsic values. Parenting in early and late adolescence predicts values internalization in emerging adulthood. Abstract This study explored parenting styles and values internalization. Perceived parenting styles were measured in Grade 7 ( n =749) and Grade 12 ( n =468), and values in Grade 12 ( n =271) and one year post-school ( n =291). We measured three aspects of valuing: priority (extrinsic, intrinsic importance); regulation (controlled, autonomous); and successful enactment of values (success). Mothers’ authoritative parenting in Grade 7 predicted increased importance and autonomous regulation of values one year post-school. Fathers’ authoritative parenting in Grade 7 predicted decreased importance of extrinsic values. Fathers’ permissive parenting in Grade 7 predicted decreased importance of intrinsic values. Authoritarian parenting in Grade 12 predicted more controlled values regulation post-school, particularly for extrinsic values. Parenting in early and late adolescence predicts values internalization in emerging adulthood.
Covariance pattern mixture models for multivariate longitudinal data with application to the Health and Retirement Study

We propose a novel approach for modeling multivariate longitudinal data in the presence of unobserved heterogeneity for the analysis of the Health and Retirement Study (HRS) data. Our proposal can be cast within the framework of linear mixed model with discrete individual random intercepts, but differently from the standard formulation, the proposed Covariance Pattern Mixture Model (CPMM) does not require the usual local independence assumption; therefore, it is able to simultaneously model the heterogeneity, the association among the responses and the temporal dependence structure. We focus on the investigation of temporal patterns related to the cognitive functioning in retired American respondents, aiming to understand whether its behaviour can be affected by some individual socio-economical characteristics and whether it is possible to identify some homogenous groups of respondents that share a similar cognitive profile, so that opportune policy interventions can be addressed. Results identify three homogenous clusters of individuals with specific cognitive functioning, consistent with the class conditional distribution of the covariates. The flexibility of CPMM allows for a different contribution of each regressor on the responses according to group membership. In so doing, the identified groups receive a global and punctual phenomenal characterization.
Type-I Ge∕Ge1−x−ySixSny strained-layer heterostructures with a direct Ge bandgap

The electronic properties of Ge∕Ge1−x−ySixSny strained-layer heterostructures are predicted theoretically. It is found that a lattice-matched system with fully strained Ge layers and relaxed Ge1−x−ySixSny alloys can have a direct fundamental bandgap with spatial localization in the Ge layers (type I). The Si and Sn concentrations for which such a direct bandgap obtains are close to those that have already been experimentally demonstrated [M. Bauer, C. Ritter, P. A. Crozier, J. Ren, J. Menendez, G. Wolf, and J. Kouvetakis, Appl. Phys. Lett. 83, 2163 (2003)]. The required level of tensile strain in the Ge layers is compatible with Si–Ge technology. The predicted direct bandgap values are as high as 0.6eV.
Magnetism in layered Ruthenates

In this thesis, the magnetism of the layered Ruthenates has been studied by means of different neutron scattering techniques. Magnetic correlations in the single-layer Ruthenates of the series Ca2-xSrxRuO4 have been investigated as function of Sr-concentration (x=0.2 and 0.62), temperature and magnetic field. These inelastic neutron scattering studies demonstrate the coexistence of ferromagnetic paramagnon scattering with antiferromagnetic fluctuations at incommensurate wave vectors. The temperature dependence of the amplitudes and energies of both types of excitations indicate the proximity to magnetic instabilities; their competition seems to determine the complex behavior of these materials. In Ca1.8Sr0.2RuO4, which shows a metamagnetic transition, the ferromagnetic fluctuations are strongly suppressed at low temperature, but appear at higher temperature or application of a magnetic field. In the high-field phase of Ca1.8Sr0.2RuO4 above the metamagnetic transition, a ferromagnetic magnon dominates the excitation spectrum. Polarized neutron scattering revealed the existence of a very broad signal around the zone centre, in addition to the well-known incommensurate excitations at Q=(0.3,0.3,0) in the unconventional superconductor Sr2RuO4. With this additional contribution, it is possible to set up a general model for the Q-dependent magnetic susceptibility, which is well consistent with the results of other measurement methods that do not resolve the Q-dependence. Upon doping with Ti, the incommensurate fluctuations are enhanced, in particular near the critical concentration for the onset of magnetic order, but no divergence down to very low temperature is observed. In the bilayer Ti-doped Sr3Ru2O7, the existence of magnetic order with a propagation vector of about (1/4 ,1/4 ,0) has been discovered and characterized in detail. Above and below TN, excitations at this wave vector and another one, related to Sr3Ru2O7, have been observed. Furthermore, in a polarized neutron diffraction study, the spin density distribution of Ca3Ru2O7 has been determined.
Effect of cilia-induced surface velocity on cerebrospinal fluid exchange in the lateral ventricles

Ciliary motility disorders are known to cause hydrocephalus. The instantaneous velocity of cerebrospinal fluid (CSF) flow is dominated by artery pulsation, and it remains unclear why ciliary dysfunction results in hydrocephalus. In this study, we investigated the effects of cilia-induced surface velocity on CSF flow using computational fluid dynamics. A geometric model of the human ventricles was constructed using medical imaging data. The CSF produced by the choroid plexus and cilia-induced surface velocity were given as the velocity boundary conditions at the ventricular walls. We developed healthy and reduced cilia motility models based on experimental data of cilia-induced velocity in healthy wild-type and Dpcd-knockout mice. The results indicate that there is almost no difference in intraventricular pressure between healthy and reduced cilia motility models. Additionally, it was found that newly produced CSF from the choroid plexus did not spread to the anterior and inferior horns of the lateral ventricles in the reduced cilia motility model. These findings suggest that a ciliary motility disorder could delay CSF exchange in the anterior and inferior horns of the lateral ventricles.
Does competitive winning increase subsequent cheating?

In this preregistered study, we attempted to replicate and substantially extend a frequently cited experiment by Schurr and Ritov, published in 2016, suggesting that winners of pairwise competitions are more likely than others to steal money in subsequent games of chance against different opponents, possibly because of an enhanced sense of entitlement among competition winners. A replication seemed desirable because of the relevance of the effect to dishonesty in everyday life, the apparent counterintuitivity of the effect, possible problems and anomalies in the original study, and above all the fact that the researchers investigated only one potential explanation for the effect. Our results failed to replicate Schurr and Ritov's basic finding: we found no evidence to support the hypotheses that either winning or losing is associated with subsequent cheating. A second online study also failed to replicate Schurr and Ritov's basic finding. We used structural equation modelling to test four possible explanations for cheating—sense of entitlement, self-confidence, feeling lucky and inequality aversion. Only inequality aversion turned out to be significantly associated with cheating.
Walking on water: revisiting the role of water in articular cartilage biomechanics in relation to tissue engineering and regenerative medicine

The importance, and the difficulty, of generating biosynthetic articular cartilage is widely recognized. Problems arise from obtaining sufficient stiffness, toughness and longevity in the material and integration of new material into existing cartilage and bone. Much work has been done on chondrocytes and tissue macromolecular components while water, which comprises the bulk of the tissue, is largely seen as a passive component; the ‘solid matrix’ is believed to be the main load-bearing element most of the time. Water is commonly seen as an inert filler whose restricted flow through the tissue is believed to be sufficient to generate the properties measured. We propose that this model should be turned on its head. Water comprises 70–80% of the matrix and has a bulk modulus considerably greater than that of cartilage. We suggest that the macromolecular components structure the water to support the loads applied. Here, we shall examine the structure and organization of the main macromolecules, collagen, aggrecan and hyaluronan, and explore how water interacts with their polyelectrolyte nature. This may inform the biosynthetic process by identifying starting points to enable developing tissue properties to guide the cells into producing the appropriate macromolecular composition and structure.
Poincar\'e/Koszul duality

We prove a duality for factorization homology which generalizes both usual Poincar\'e duality for manifolds and Koszul duality for $\mathcal{E}_n$-algebras. The duality has application to the Hochschild homology of associative algebras and enveloping algebras of Lie algebras. We interpret our result at the level of topological quantum field theory.