Miracle-dz/UniFAR-FTUs huggingface dataset:
The complete chloroplast genome of Salix psamaphila, a desert shrub in northwest China

Abstract Salix psammophila C. Wang & Chang Y. Yang is a desert plant species distributed in Northwest China. Here, we report the complete choroplast genome sequences in order to enrich its genetic resource. The total genome is 155,278 bp in length and contains a typical quadripartite structure, including a large single copy (LSC) region of 84,457 bp, a small single copy (SSC) region of 15,891 bp, and a pair of inverted repeats (IRs) of 27,465 bp. There were 130 genes in the genome, including 85 protein-coding genes, 37 tRNA genes and eight rRNA genes. The overall CG content in the plastome of S. psammophila is 36.72%. The phylogenetic tree based on 18 complete plastomes of Salicaceae support close relationships among S. psammophila, Salix taoensis, and Salix rehderiana.
Priority Oral Health Program and List Management Protocols - NSW Department of Health

Priority Oral Health Program and List Management Protocols , The purpose of the Priority Oral Health Program and Wait List Management Protocols is to establish a clear and consistent patient flow pathway for eligible NSW residents who access general public dental services. Policy has been amended to update recommendations to change 'urgent dentures' to a Code D with the recommended maximum waiting time of 9 months, and 'chronic disease conditions' to a Code C with the recommended maximum waiting time of 6 months to align existing public dental work practices to the 2008 Commonwealth Dental Health program strategies.
Proteomic analysis of mature kernel aleurone layer of Triticum spelta and three wheat related species

Background : A proteomics approach was used to reveal major protein differences in the aleurone layer (AL) of mature grain between four wheat species: T. aestivum and T. spelta (6X), T. durum (4X), and T. monococum (2X). Materiel and methods: In each species, one representative cultivar was used for total AL protein extraction. Two-dimensional electrophoresis of AL proteins revealed 1380, 1355, 1120 and 973 Coomassie stained spots, respectively. Result: A total of 334 spots showed quantitative or qualitative (presence/absence) differences between the four cultivars. T. aestivum and T. spelta had 90.9% and 92.6% of their AL proteome respectively, identical to the other species. Only 25 spots were significantly different between the two hexaploid cultivars. AL proteins encoded by the A genome were mainly expressed in polyploid species. Percentages of proteins encoded by A genome was 85%, in tetraploid species and 70% in the hexaploid spelt. Eighty for proteins are identified by mass spectrometry and data mining and were classified in five bbiological processes and (12) functional categories. Conclusion: The major differences between species were particularly due to storage globulins (22, 11 and 1 of Glo-3, Glo-3B, Glo-3C, respectively), stress related proteins including antioxidant proteins like 1-Cys peroxiredoxin and manganese superoxide dismutase, defence proteins like xylanase, serpin3 and heat shock proteins.
Sex expression and genotypic sex ratio vary with region and environment in the wetland moss Drepanocladus lycopodioides

Sex ratio variation is common among organisms with separate sexes. In bryophytes, sex chromosome segregation at meiosis suggests a balanced progeny sex ratio. However, most bryophyte populations exhibit female-biased phenotypic sex ratios based on the presence of reproductive structures on gametophytes. Many bryophyte populations do not form sexual organs, and genotypic sex ratio variation in such populations is mostly unknown. We tested sex expression, and phenotypic and genotypic sex ratios against environmental parameters in natural populations of the unisexual wetland moss Drepanocladus lycopodiodes at 11 sites in each of three regions in southern Sweden. We identified sex in 660 individual ramets, based on sexual structures, when present, or with a specifically designed molecular marker, when absent. All regions exhibited a female bias in phenotypic and genotypic sex ratios. Sex ratio biases and sex expression differed between regions. Sex ratios were less female-biased in larger patches. Wetter patches exhibited a stronger female bias in genotypic sex ratio and lower sex expression. This is the first evidence of environmental effects on genotypic sex ratio in mosses. A higher frequency of females in wet patches could be due to higher female resource demands for sporophyte production or higher male sensitivity to wetness. A higher incidence of females than males in moister sites aligns with female flowering plants, but differs from reproductive bryophytes in drier environments. Taken together with previous results, our data indicate that sex ratio variation and its drivers differ among species, their life histories and environments.
Novel regulation from novel interactions: Identification of an RNA sponge that controls the levels, processing and efficacy of the RoxS riboregulator of central metabolism in Bacillus subtilis

Small RNAs (sRNAs) are a taxonomically-restricted but transcriptomically-abundant class of post-transcriptional regulators. While potentially of importance, we know the function of few. This is in no small part because we lack global-scale methodology enabling target identification, this being especially acute in species without known RNA meeting point proteins (e.g. Hfq). We apply a combination of psoralen RNA cross-linking and Illumina-sequencing to identify RNA-RNA interacting pairs in vivo in Bacillus subtilis, resolving previously well-described interactants. Although sRNA-sRNA pairings are rare (compared with sRNA/mRNA), we identify a robust example involving the unusually conserved sRNA (RoxS/RsaE) and an unstudied sRNA that we term Regulator of small RNA A (RosA). This interaction is found in independent samples across multiple conditions. Given the possibility of a novel associated regulatory mechanism, and the rarity of well-characterised bacterial sRNA-sRNA interactions, we mechanistically dissect RosA and its interactants. RosA we show to be a sponge RNA, the first to be described in a Gram-positive bacterium. RosA interacts with at least two sRNAs, RoxS and FsrA. Unexpectedly, it acts differently on each. As expected of a sponge RNA, FsrA is sequestered by RosA. The RosA/RoxS interaction is more complex affecting not only the level of RoxS but also its processing and efficacy. Importantly, RosA provides the condition-dependent intermediary between CcpA, the key regulator of carbon metabolism, and RoxS. This not only provides evidence for a novel, and functionally important, regulatory mechanism, but in addition, provides the missing link between transcriptional and post-transcriptional regulation of central metabolism.
Systematics of Rudbeckia Subgenera Macrocline and Laciniata (Asteraceae: Heliantheae: Rudbeckiinae).

Rudbeckia. a North American genus, consists of approximately 21 species of annual and perennial herbs. Rudbeckia is separated into three subgenera [i.e. Laciniata (n=19[18]), Macrocline (n=18) and Rudbeckia (n=19)] based on morphological, cytological, chemical, and phylogenetic data. The present research includes a systematic study of subgenera Laciniata and Macrocline. Also included is a phylogenetic analysis of the coneflower genera (Dracopis. Echinacea. Ratibida. and Rudbeckia). Subgenus Laciniata contains Rudbeckia laciniata. which is composed of five morphological and geographical overlapping varieties. These include var. ampla. var. bipinnata. var. digitata. var. heterophvlla. and var. laciniata. Twelve species are included in subg. Macrocline. Based on the morphometric and cladistic analysis, subg. Macrocline is divided into two sections. The first is sect. Askomia, which contains the rayless coneflowers (R. alpicola. R. montana, and R. occidentalis). The second is sect. Macrocline which comprises the remaining species (R. auriculata. R. californica. R. qlaucescens. R. klamathensis (nom. nov.), R. maxima, R. mohrii, R. nitida. R. scabrifolia. and R.
Synchronized eye movements predict test scores in online video education

Significance Education is increasingly delivered online, but are students actually paying attention? Here we demonstrate that efficacy of video instruction can be assessed remotely with standard web cameras. Specifically, we show that attentive students have similar eye movements when watching instructional videos and that synchronization of eye movements is a good predictor of individual learning performance. Measuring synchronization of eye movements while preserving privacy, as we have shown here, has the potential to make online education adaptive to attentional state and advance mechanistic studies on the efficacy of different online education formats. Attention has become a commodity online. With the increasing abundance of video content, remote sensing of attention at scale may be relevant beyond education, including entertainment, advertising, and politics. Experienced teachers pay close attention to their students, adjusting their teaching when students seem lost. This dynamic interaction is missing in online education. We hypothesized that attentive students follow videos similarly with their eyes. Thus, attention to instructional videos could be assessed remotely by tracking eye movements. Here we show that intersubject correlation of eye movements during video presentation is substantially higher for attentive students and that synchronized eye movements are predictive of individual test scores on the material presented in the video. These findings replicate for videos in a variety of production styles, for incidental and intentional learning and for recall and comprehension questions alike. We reproduce the result using standard web cameras to capture eye movements in a classroom setting and with over 1,000 participants at home without the need to transmit user data. Our results suggest that online education could be made adaptive to a student’s level of attention in real time.
ESTUDO BIOLÓGICO-PESQUEIRO DO ESPADARTE, Xiphias gladius LINNAEUS, 1758, NO SUDESTE E SUL DO BRASIL (1971 A 1981)

The swordfish represents about 24% of the total catch of the Brazilian longliners which operate in the area 20°-33°S and 039°-050°W, being usually the second most caught species. Commonly, the values of yield, catch per unit of effort and average weight were higher in the third quarter (winter). The great increase of yield and catch per unit of effort verified in 1980 was probably due to changes of the bait used and the schedule of fishery. The analysis of these data suggests a potential to be better studied. The obtained data included the sex ratio: 1/1.14; the dressed weight/length: Pe = 1.24 x 10-5C3.04; and the total weight/dressed weight: Pt = 1.17 Pe relationships. The catch comprehended specimens between 50 and 290 cm, the sizes of 85 to 185 cm being the most frequent. In this region the spawning of the species occurs from the middle of the fourth to the end of the first quarter, mainly in January-February. During this period the fleet operated in the area 20°-28°S and 039°-048°W. Three species of ectoparasites of the genus Pennella and three commensals of the genus Remora were identified, and the result of the action of the predators was observed.
Potential soil transmission of a novel Candidatus Liberibacter strain detected in citrus seedlings grown in soil from a huanglongbing infested citrus grove

Candidatus Liberibacter spp. are Alphaproteobacteria associated with plants and psyllid vectors. Most cause plant diseases, including Ca Liberibacter asiaticus (Las) associated with citrus huanglongbing (HLB). Replacing HLB-infected by Las-free citrus trees results in fast re-infection despite psyllid control. To check if HLB could be soil-borne, we performed an insect-free greenhouse-experiment with 130 mandarin seedlings in two citrus-grove soils (A and B), non-autoclaved or autoclaved. Liberibacter-specific 16S-rDNA PCR primers to detect Las were used to search for Ca. Liberibacter spp. in mandarin leaves. Seven plants grown in non-autoclaved soil B showed HLB-like symptoms and tested positive after 2.5 and 8.5 months using three different primer systems: two based on the 16S-rDNA gene (primers HLBas/HLBr and OI2c/OI1) and one based on the rplA/rplJ gene (primers LAA2/LAJ5). DNA segments from these plants amplified by primers OI2c/OI1 were cloned and sequenced; they were 95.9 % similar to Las and 94.8% to Ca. Liberibacter africanus (Laf). The DNA product from Liberibacter-group specific PCR primers for the rplA/rplJ gene was 87.6% similar to that of Las and 78.2% of Laf. As the strain obtained originated from soil and was different from existing Ca. Liberibacter species, this strain may be a new species.
Regeneration of Neem Plantlet by in Vitro Clonal Micropropagation

The present study was carried out during 2014-15 with Neem (Azadirachta indica) is an evergreen tropical forest tree that is a renewable source of various useful products, to investigate optimal concentrations and combinations of plant growth regulators in the medium for efficient micropropagation. The present observation revealed that the highest callusing response 22.3±0.92% was observed in MS medium supplemented with 0.3 mg/l 2, 4-D; 0.3 mg/l Kn and 0.3 mg/l NAA in leaf explant and in seed explant it is 42.50.97% was observed in MS medium supplemented with 0.5 mg/l of each 2, 4-D and BAP. The highest shoot regenerating capacity (94%) was recorded in MS medium supplemented with 1.0+0.5 mg/l (BAP+NAA). Best rooting (95%) was observed in 1⁄2 strength of MS medium supplemented with 3 mg/l NAA and the highest number of average roots per micro shoots and average length of root were 15 and 4.9 cm respectively. Acclimatization and hardening was successful with survival rate of 87.5%. Keyword: Azadirachta indica, MSMurashige and Skoog, explant, harmones, micropropagation
The effect of dropout intervention strategies on the graduation rate in selected rural school districts in New York State

i Acknowledgements . ii List of Tables . ix Chapter 1: Introduction . 1 Background . 1 Research Problem . 3 Purpose Statement . 4 Research Questions . 5 Definition of Terms Used for This Study . 6 Significance of the Study . 8 Limitations of the Study.8 Organization of the Study . 9 Chapter 2: Literature Review . 10 Reform Efforts . 12 Leadership . 15 Leadership for change . 19 New York State Regents Reform Agenda . 20 Public School Enrollment Data . 22 New York State enrollment data . 23 Economic Costs of High School Dropouts . 23 Economic Benefits of High School Graduation.24 National High School Graduation Data . 25
ASSESSMENT AND ENHANCING ADHERENCE TO TREATMENT REGIMEN IN TUBERCULOSIS OUT PATIENTS Research Article

Objectives: The objectives of the study were to describe the pattern and monitoring the medication compliance of anti-tubercular used in patients and to assess the knowledge among patients regarding their medications. Methods: During the 7 months prospective study, a suitably designed patient data collection form was prepared in consultation with the physician and clinical pharmacist, also referring standard textbooks and journals, which include information of patient demographics details such as age, gender, education, duration of disease, and medications history. Results were analyzed by using Wilcoxon signed-ranks test. Key findings: Among the 39 patients who completed the 2 months follow up study, there was statistically significant increase in medication adherence score from baseline. Baseline with first follow up revealed a mean increase in medication adherence level of 1.718 ± 0.759 and (p < 0.0001) which is statistically significant. Baseline to second follow up show a mean increase of 2.435 ± 0.852 and (p < 0.0001) which is statistically significant. The major reasons to stop medication were as follows: 23(58.97%) patients said that they stopped/miss the medication because of forgetfulness, 12(30.77%) patients stopped/miss medication because high cost of medications, 20(51.28%) patients stop/miss medication because Lack of access to hospital/drug store. Conclusions: The finding of this study may suggest that a well-structured tuberculosis patient counseling by pharmacist result in better medication adherence which may indirectly result in better clinical outcome.
In-Silico Analysis of Grapevine Germin like Protein (VvGLP3) and its Probable Role in Defense Against Powdery Mildew Disease

Germin like proteins (GLPs) is a heterogeneous group of proteins that are part of Cupin superfamily. Various GLPs of Grapevine (VvGLP3) have been identified and characterized at molecular but not at the structural level. This study analyzes the structure of VvGLP3 to elucidate its role in defense against powdery mildew disease caused by Erysiphe necator. Physiochemical analysis through the high aliphatic index (AI), low instability index and grand average of hydropathy (GRAVY) reveal that VvGLP3 is heat stable hydrophobic protein. InterProScan was used to check the similarity of VvGLP3 to other GLPs and TMHMM indicates that VvGLP3 is a transmembrane protein. Secondary structure of VvGLP3 reveals that it consists of random coils followed by extended α-helix and β-trends and I-TASSER was employed to construct a 3-D structure of VvGLP3. A molecular docking approach was applied to study molecular interaction and binding pattern by Auto Dock Vina. I-TASSER and SOPMA were used to determine the functionally important residues and their role. In the present study, molecular docking binding affinity energy is from -1.5 to -1.3 of germin like protein with manganese (Mn+2) co-factor which depict its role in defense against powdery mildew disease.
Characterization of complete chloroplast genome of traditional Chinese medical plants Paris Mairei and its phylogenetic positions

Abstract The genus Paris (Liliaceae) has been used for traditional medicine in China. The wild Paris was on the verge of exhaustion due to illegal and immoderate exploitation coupled with environmental pollution. In order to alleviate resource pressure, Paris mairei can be considered as alternative sources. Here, we report the complete chloroplast genome of Paris mairei. The genome is 162,736 bp in length including a small single-copy region (SSC, 12,908 bp) and a large single-copy region (LSC, 84,286 bp) separated by a pair of inverted repeats (IRs; 32,771 bp). The genome contained 113 genes, including 79 protein-coding genes, 4 ribosomal RNA genes, and 30 tRNA genes. Among these genes, 16 harboured a single intron, and 2 contained a couple of introns. The overall G + C content of the cpDNA is 37.1%, while the corresponding values of the LSC, SSC, and IR regions are 35.7, 32.1, and 39.9%, respectively. The complete chloroplast genome sequence of Paris mairei will provide a useful resource for the conservation genetics of this species as well as for the phylogenetic studies for the genus Paris.
On the asymptotic equivalence between the radon and the hough transforms of digital images

Although characterized by different mathematical definitions, both the Radon and the Hough transforms ultimately take an image as input and provide, as output, functions defined on a preassigned parameter space, i.e., the so-called either Radon or Hough sinograms. The parameters in these two spaces describe a family of curves, which represent either the integration domains considered in the Radon transform, or the kind of curves to be detected by the Hough transform. It is heuristically known that the Hough sinogram converges to the corresponding Radon sinogram when the discretization step in the parameter space tends to zero. By considering generalized functions in multi-dimensional setting, in this paper we give an analytical proof of this heuristic rationale when the input grayscale digital image is described as a set of grayscale points, that is, as a sum of weighted Dirac delta functions. On these grounds, we also show that this asymptotic equivalence may have a valuable impact on the image reconstruction problem of inverting the Radon sinogram recorded by a medical imaging scanner.
Distribución de Vanilla planifolia Jacks. ex Andrews y acciones para su conservación en la Huasteca Potosina

La vainilla (Vanilla planifolia) es una de las orquídeas más ampliamente utilizada desde la época precolombina, actualmente, su estatus de conservación corresponde a la categoría de Sujeta a Protección Especial. Los objetivos de esta investigación fueron identificar su distribución actual y potencial en la Huasteca Potosina; y diseñar acciones para su conservación. Para ello, se realizaron consultas en herbarios, recorridos de campo, entrevistas con los productores de vainilla y talleres participativos con habitantes locales. Se llevó a cabo un análisis espacial basado en sistemas de información geográfica, para conocer las características ambientales de los sitios con presencia de la especie y se modelizó su distribución potencial. En la Huasteca Potosina, se ubicaron 28 sitios con presencia del taxón bajo estudio, la mayoría en sistemas agroforestales tradicionales y, menor proporción, en los relictos de selva mediana que aún persisten en la región, anclados a los tutores que les proveen el soporte necesario. Su distribución potencial se estimó en 85.5 km2. El germoplasma sin procesos de domesticación y adaptado a las condiciones ambientales que se identificaron, tiene posibilidades de ser conservado. Los poseedores de este recurso genético consideran que una Unidad de Manejo de la Vida Silvestre sería la forma más adecuada para lograr su conservación in situ.