Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish

Background: Semaphorin (Sema)/Plexin (Plxn) signaling is important for many aspects of neuronal development, however, the transcriptional regulation imposed by this signaling pathway is unknown. Previously, we identified an essential role for Sema6A/PlxnA2 signaling in regulating proliferation and cohesion of retinal precursor cells (RPCs) during early eye development. This study used RNA isolated from control, Sema6A‐deficient and PlxnA2‐deficient zebrafish embryos in a microarray analysis to identify genes that were differentially expressed when this signaling pathway was disrupted. Results: We uncovered a set of 58 transcripts, and all but 1 were up‐regulated in both sema6A and plxnA2 morphants. We validated gene expression changes in subset of candidates that are suggested to be involved in proliferation, migration or neuronal positioning. We further functionally evaluated one gene, rasl11b, as contributing to disrupted proliferation in sema6A and plxna2 morphants. Our results suggest rasl11b negatively regulates proliferation of RPCs in the developing zebrafish eye. Conclusions: Microarray analysis has generated a resource of target genes downstream of Sema6A/PlxnA2 signaling, which can be further investigated to elucidate the downstream effects of this well‐studied neuronal and vascular guidance signaling pathway. Developmental Dynamics 246:539–549, 2017. © 2017 Wiley Periodicals, Inc.
Fluorescent Labeling of Yeast

This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.
Molecularly imprinted solid phase extraction for trace analysis of diazinon in drinking water.

Amongst organophosphate pesticides, the one most widely used and common environmental contaminant is diazinon; thus methods for its trace analysis in environmental samples must be developed. Use of diazinon imprinted polymers such as sorbents in solid phase extraction, is a prominent and novel application area of molecular imprinted polymers. For diazinon extraction, high performance liquid chromatography analysis was demonstrated in this study. During optimization of the molecular imprinted solid phase extraction procedure for efficient solid phase extraction of diazinon, Plackett-Burman design was conducted. Eight experimental factors with critical influence on molecular imprinted solid phase extraction performance were selected, and 12 different experimental runs based on Plackett-Burman design were carried out. The applicability of diazinon imprinted polymers as the sorbent in solid phase extraction, presented obtained good recoveries of diazinon from LCgrade water. An increase in pH caused an increase in the recovery on molecular imprinted solid phase extraction. From these results, the optimal molecular imprinted solid phase extraction procedure was as follows: solid phase extraction packing with 100 mg diazinon imprinted polymers; conditioning with 5 mL of methanol and 6 mL of LC-grade water; sample loading containing diazinon (pH=10); washing with 1 mL of LC-grade water, 1 mL LCgrade water containing 30% acetonitrile and 0.5 mL of acetonitrile, respectively; eluting with 1 mL of methanol containing 2% acetic acid. The percentage recoveries obtained by the optimized molecular imprinted solid phase extraction were more than 90% with drinking water spiked at different trace levels of diazinon. Generally speaking, the molecular imprinted solid phase extraction procedure and subsequent high performance liquid chromatography analysis can be a relatively fast and proper approach for qualitative and quantitative analysis of diazinon in drinking water.
Genetic control of the cytotoxic T cell response to SV40 tumor-associated specific antigen.

The H-2 gene complex controls the ability of mice to generate cytotoxic T cells to the SV40 tumor-associated specific antigen (TASA). Mice of six H-2 haplotypes (H-2b, d, f, k, q, and s) were challenged in vivo with syngeneic simian virus 40 (SV40)-transformed cells. By testing splenic lymphocytes in a 51Cr cytotoxic test, only H-2b mice were able to generate cytotoxic T cells specific for syngeneic SV40-transformed target cells. In contrast, immune lymphocytes from both H-2b and H-2k mice assayed after secondary in vitro restimulation lysed SV40-transformed target cells of appropriate H-2 genotype. Popliteal lymph node cells from H-2b, k, and d mice, immunized by footpad injection of SV40 and then incubated for 14 days in vitro before assay, are cytotoxic for syngeneic SV40-transformed cells. With this immunization protocol, H-2b mice respond to SV40 TASA in association with both the H-2 K and D locus-coded molecules, whereas H-2k mice respond to SV40 TASA in association with the H-2 K molecule only and H-2d mice respond to SV40 TASA in association with the H-2 D gene product. SV40 TASA-immune lymphocytes from various F1 (responder × nonresponder) mice were also unable to lyse syngeneic H-2Kd or H-2Dk SV40-transformed target cells. Inhibition of the response to SV40 TASA in association with H-2Dd is found when lymphocytes from SV40 TASA-immune F1 hybrid (H-2b × H-2d) or H-2 congenic recombinant (H-2KbDd) mice are analyzed. A dominant suppression or recessive helper mechanism is hypothesized to account for these results.
Caring For Hispanic Patients Interactively: Simulations and Practices for Allied Health Professionals

Healthcare providers have a new tool for navigating the challenges of cross-cultural communication when treating Hispanic patients: CHISPA e-learning environment. CHISPA, which means "spark" in Spanish, stands for Caring for the Hispanic Patient Interactively: Simulations and Practices for Allied Health Professionals. It is a computer-based training program that provides solutions to both cultural and linguistic barriers to care. The program allows users to interact with Hispanic patients in realistic patient-care scenarios and receive feedback on their choices, along with a wealth of information on cultural variables, advice on overcoming language barriers, and language exercises to facilitate learning medical terms and expressions in Spanish.
ISI at the SIGMORPHON 2017 Shared Task on Morphological Reinflection

We present a system for morphological reinﬂection based on the LSTM model. Given an input word and morphosyntactic descriptions, the problem is to classify the proper edit tree that, applied on the input word, produces the target form. The proposed method does not require human deﬁned features and it is language independent also. Currently, we evaluate our system only for task 1 without using any external data. From the test set results, it is found that the proposed model beats the baseline on 15 out of the 52 languages in high resource scenario. But its performance is poor when the training set size is medium or low.
Discovering LTL based business rules from Event Logs

This thesis focuses on the discovery of linear temporal logic business rules from event logs. Linear temporal logic is used to describe business processes in a declarative way. The developed application LTLMiner aims at improving the performance of an existing tool TLQC used for business rule discovery. The thesis explains the solution and compares the performance between the LTLMiner and TLQC.
Immunochemistry for high-throughput screening of human exhaled breath condensate (EBC) media: implementation of automated quanterix SIMOA instrumentation

Immunochemistry is an important clinical tool for indicating biological pathways leading towards disease. Standard enzyme-linked immunosorbent assays (ELISA) are labor intensive and lack sensitivity at low-level concentrations. Here we report on emerging technology implementing fully-automated ELISA capable of molecular level detection and describe application to exhaled breath condensate (EBC) samples. The Quanterix SIMOA HD-1 analyzer was evaluated for analytical performance for inflammatory cytokines (IL-6, TNF-α, IL-1β and IL-8). The system was challenged with human EBC representing the most dilute and analytically difficult of the biological media. Calibrations from synthetic samples and spiked EBC showed excellent linearity at trace levels (r2 > 0.99). Sensitivities varied by analyte, but were robust from ~0.006 (IL-6) to ~0.01 (TNF-α) pg ml−1. All analytes demonstrated response suppression when diluted with deionized water and so assay buffer diluent was found to be a better choice. Analytical runs required ~45 min setup time for loading samples, reagents, calibrants, etc., after which the instrument performs without further intervention for up to 288 separate samples. Currently, available kits are limited to single-plex analyses and so sample volumes require adjustments. Sample dilutions should be made with assay diluent to avoid response suppression. Automation performs seamlessly and data are automatically analyzed and reported in spreadsheet format. The internal 5-parameter logistic (pl) calibration model should be supplemented with a linear regression spline at the very lowest analyte levels, (<1.3 pg ml−1). The implementation of the automated Quanterix platform was successfully demonstrated using EBC, which poses the greatest challenge to ELISA due to limited sample volumes and low protein levels.
Prolonged inhibition of bacterial protein synthesis abolishes Salmonella invasion

We have found that prolonged inhibition of bacterial protein synthesis abolishes the ability of Salmonella typhimurium to enter HEp-2 cells. Our results suggest that an essential invasion factor has a functional half-life that is seen as a gradual loss of invasiveness in the absence of protein synthesis. Therefore, Salmonella invasiveness appears to be a transient phenotype that is lost unless protein synthesis is maintained. This finding may explain why salmonellae grown to stationary phase lose their ability to enter cultured cells. In addition, a short-lived capacity to enter cells may be important during infection so that bacterial invasiveness is limited to certain times and host sites during pathogenesis.
Best known PSLs for binary sequences from bit length 71 through 100

This paper develops a new evolutionary algorithm for generating low autocorrelation binary sequences. These sequences are of interest in pulse compression technique. The proposed algorithm is fast enough to yield optimum or near optimum codes. The generated sequences were compared to the best literature and were seen that its results are better than the others. This suggested method could change 11 rows of the previous best known PSLs table, whereas the previous literature could change only one record. These records were combined with the best results reported in the papers to produce a new best minimal-PSL binary sequence table for bit lengths 71 through 100.
Theoretical modeling on luminescent down-shifting process: A Discussion on luminescent molecule design

A model was developed to describe the luminescent down-shifting (LDS) process in photovoltaic application. The model links the J<sub>sc</sub> enhancement (J<sub>LDS</sub>%) of the solar cell to the photophysical properties including absorption (λ<sub>abs</sub>) and emission (λ<sub>em</sub>) wavelengths, Stokes shift (Δλ) and spectral cutting point (λ<sub>SCP</sub>), as well as fluorescence quantum yield (Φ<sub>f</sub>), of the luminescent molecules. It suggests that Φ<sub>f</sub> is a more crucial parameter than λ<sub>abs</sub> and λ<sub>em</sub>. It also suggests that λ<sub>SCP</sub> is a better parameter than Δλ on describing J<sub>LDS</sub>%. The model is tested with luminescent molecules and the results are consistent with the experimental results.
Common diagnostic tests: use and interpretation.

Find loads of the common diagnostic tests use and interpretation book catalogues in this site as the choice of you visiting this page. You can also join to the website book library that will show you numerous books from any types. Literature, science, politics, and many more catalogues are presented to offer you the best book to find. The book that really makes you feels satisfied. Or that's the book that will save you from your job deadline.
Addresses to the Federal Assembly of the Russian Federation by Russian presidents, 2008–2012: comparative analysis

Every year Russian presidents deliver an address to the Federal Assembly of the Russian Federation, which includes prominent politicians, ministers, government officials and various other public figures. They set the president’s public agenda for the forthcoming year and give an insight into the specific proposals and strategies that are to be implemented. These addresses are available in English in Kremlin’s website http://eng.kremlin. ru/transcripts/messages
Explicit TCP window adaptation in a shared memory architecture

A multiple (priority) queueing system allows a network node to manage the queueing of packets in such a way that higher priority packets will always be served first, low priority packets will be discarded when the queue is full, and for same‐priority packets any interference between them will be prevented. This paper describes a TCP window control scheme for a shared memory device that has buffer memory logically organized into multiple queues. To handle changing queue traffic loads, the shared memory device uses a dynamic buffer threshold mechanism to allocate buffer space to the queues. The TCP window control scheme allows the receiver's advertised window size in ACK packets to be modified at the network queue in order to maintain the queue size at a computed dynamic threshold. Copyright © 2003 John Wiley & Sons, Ltd.
Transfer of a CD4+ Th1 cell line to nude mice effects clearance of Rhodococcus equi from the lung

Rhodococcus equi, and intracellular respiratory pathogen, causes sever e granulomatous pneumonia in humans with AIDS and in young horses. Pulmonary clearance of R. equi requires functional CD4+ T cells and gamma interferon (IFN-gamma) expression from bronchial lymph node cells. The purpose of this study was to investigate whether R. equi-specific CD4+ Th1 cells could effect clearance of R. equi from the lung. Adoptive transfer of a clearance of R. equi from the lungs. In contrast, mice transfused with a R. equi-specific CD4+ Th2 cell line expressed interleukin-4 but not IFN-gamma mRNA, failed to clear pulmonary infection, and developed granulomas in the lung. Control mice, which did not receive cells, did not produce IFN-gamma or interleukin-4 and developed small pulmonary granulomas. These results clearly show that a Th1 response is sufficient to effect pulmonary clearance of R. equi.
Exposing the Hidden-Web Induced by Ajax

AJAX is a very promising approach for improving rich interactivity and responsiveness of web applications. At the same time, AJAX techniques increase the totality of the hidden web by shattering the metaphor of a web ‘page’ upon which general search engines are based. This paper describes a technique for exposing the hidden web content behind AJAX by automatically creating a traditional multi-page instance. In particular we propose a method for crawling AJAX applications and building a state-flow graph modeling the various navigation paths and states within an AJAX application. This model is used to generate linked static HTML pages and a corresponding Sitemap. We present our tool called CRAWLJAX which implements the concepts discussed in this paper. Additionally, we present a case study in which we apply our approach to two AJAX applications and elaborate on the obtained results.
New Paradigms for Brain Computer Interface Based on SSVEP

A Brain Computer Interface (BCI) creates a new communication channels for disabled peoples, the classical way of steady-state visual evoked potential (SSVEP) based on (BCI) was focusing on multi-frequency and phases. In this study a novel high-speed SSVEP based on BCI, was implemented a new paradigms of SSVEP, which induced by irregular flickering with phase-tagged toward brain activity response, which effectively mean multiple frequencies combination, which lead to distinguishable brain response, were considered a closed loop system, as off-line analysis, the combining average of stimuli frequency is recommended using FFT. The frequency and feature extracted of electroencephalograms (EEG) were computed to intention detection, in this experimental study, different results between regular versus irregular had been found, represented by high amplitude with respect to conjunctive of stimuli flicker according to a patterns and frequencies. Keywords- Brain Computer Interface (BCI); steady state visual evoked potential (SSVEP); electroencephalograms (EEG); Fast Fourier Transform (FFT); Event Related Potential (ERP).
Energy-efficient caching strategy using probability-based data request for mobile environments

In mobile environments, where data is broadcasted, the caching of frequently accessed data in the battery-powered mobile terminals is a very attractive technique in terms of the energy and bandwidth efficiency. The invalidation report (IR) based caching strategy is one of the popular caching strategies by its scalability. In the IR-based caching strategy, the data consistency is maintained by using the IR which has the information of updated data. In this paper, we approach the IR-based caching strategy in view of the energy efficiency and propose the energy-efficient caching strategy. With the proposed caching strategy, redundant data request messages for the identical data decrease significantly. Simulation results show that the proposed caching strategy has a great effect on the conservation of the battery capacity of mobile terminals and the uplink bandwidth compared with the conventional IR-based caching strategy.
Application of cascaded H-bridge multilevel inverter in DTC-SVM based induction motor drive

In this paper, application of cascaded H-bridge multilevel inverter in DTC-SVM based induction motor drive is presented. Cascaded H-bridge multilevel inverter uses multiple series units of H-bridge power cells to achieve medium-voltage operation and low harmonic distortion. Each H-bridge can be implemented using a dc source, which would be available from fuel cell, batteries or ultra-capacitors. This inverter can be perceived in two arrangements, symmetrical and asymmetrical with equal and unequal dc voltage sources, respectively. The control strategy is based on DTC-SVM technique. In this scheme, first, stator voltage vector is calculated and then realized by SVM method. Contribution of multilevel inverter to the DTC-SVM scheme is led to achieve high performance motor drive. Five-level and nine-level inverter are employed to investigate the considered motor drive. Simulations are carried out in Matlab-Simulink. The results demonstrate the great performance of multilevel inverter in DTC-SVM induction motor drive.
Binary partitioning for continuous longitudinal data: categorizing a prognostic variable

We investigate a binary partitioning algorithm in the case of a continuous repeated measures outcome. The procedure is based on the use of the likelihood ratio statistic to evaluate the performance of individual splits. The procedure partitions a set of longitudinal data into two mutually exclusive groups based on an optimal split of a continuous prognostic variable. A permutation test is used to assess the level of significance associated with the optimal split, and a bootstrap confidence interval is obtained for the optimal split. Copyright © 2002 John Wiley & Sons, Ltd.
Kinematic effects of a pedicle-lengthening osteotomy for the treatment of lumbar spinal stenosis.

OBJECT Lumbar spinal stenosis (LSS) may lead to disabling neurogenic symptoms and has traditionally been treated using open laminectomy. A new technique for correcting LSS involves lengthening the lumbar pedicles through bilateral percutaneous pedicle osteotomies. In this paper, the authors' goal was to evaluate the changes in spinal canal dimensions and kinematic behavior after pedicle-lengthening osteotomies. METHODS The kinematic behavior of 8 cadaveric lumbar segments was evaluated intact and after bilateral pedicle-lengthening osteotomies at the L-4, L-5, and L-4 and L-5 levels. Testing was conducted with and without a compressive preload using a custom kinematic apparatus that allowed for 3D tracking of each vertebra during flexion-extension, right-left bending, and right-left rotation. A validated finite element (FE) spine model was used to measure the changes in the cross-sectional area of the spinal canal and neural foramen after 2-, 3-, and 4.5-mm simulated pedicle-lengthening osteotomy procedures. RESULTS The overall and segmental kinematics were not significantly altered after the pedicle-lengthening osteotomy procedure at the L-4 and/or L-5 pedicles. The kinematic signatures of the intact and lengthened states were similar for all motion pairs. The FE spine model yielded kinematics predictions within or close to the 95% confidence interval for the cadaveric data. The FE spine demonstrated substantial, pedicle length-dependent enlargement of the cross-sectional areas of the spinal canal and neural foramen after simulated pedicle lengthening. CONCLUSIONS Bilateral pedicle-lengthening osteotomies produced substantial increases in the cross-sectional areas of the spinal canal and neural foramen without significantly altering normal spinal kinematics. This technique deserves further study as a less invasive treatment option for LSS.
Molecular dynamics simulations of clusters and thin film growth in the context of plasma sputtering deposition

Carrying out molecular dynamics (MD) simulations is a relevant way to understand growth phenomena at the atomic scale. Initial conditions are defined for reproducing deposition conditions of plasma sputtering experiments. Two case studies are developed to highlight the implementation of MD simulations in the context of plasma sputtering deposition: ZrxCu1−x metallic glass and AlCoCrCuFeNi high entropy alloy thin films deposited onto silicon. Effects of depositing atom kinetic energies and atomic composition are studied in order to predict the evolution of morphologies and atomic structure of MD grown thin films. Experimental and simulated x-ray diffraction patterns are compared.
Interferon as therapy for multiple myeloma: an individual patient data overview of 24 randomized trials and 4012 patients

Many randomized trials have evaluated α‐interferon as myeloma therapy, some suggesting a benefit and others not. Most were too small to give reliable answers, so a systematic overview has been performed to provide a more reliable estimate of the effect of interferon. The main end‐points were response rates (induction trials), progression‐free survival (PFS) and overall survival (OS). Individual patient data were supplied for 24 trials involving 4012 patients, 12 induction trials (2469 patients) and 12 maintenance trials (1543 patients). In induction, response rates were slightly better with interferon (57·5% versus 53·1%, P = 0·01). PFS was better with interferon (33% versus 24% at 3 years, P < 0·00001), an effect seen in both induction (P = 0·0003) and maintenance (P < 0·00001) trials. Median time to progression was increased by about 6 months in both settings. OS was somewhat better with interferon (53% versus 49% at 3 years, P = 0·01) with median survival increased by about 4 months. This benefit was restricted to the smaller trials. The effect of interferon was not significantly related to the dose or duration of interferon or to patients' characteristics. PFS was improved with interferon, but the survival benefit, if any, was small and needs balancing against cost and toxicity.