Miracle-dz/UniFAR-FTUs huggingface dataset:
Investigation of Apoptosis-Related Gene Expression Levels in Preimplantation Biopsies as Predictors of Delayed Kidney Graft Function

Background The purpose of this study was to investigate the expression of the gene coding for the antiapoptotic molecule Bcl-2, the proapoptotic molecule Bax, and the apoptosis executor enzyme caspase-3 in preimplantation renal biopsies (PIB) as markers for delayed graft function. Methods In this prospective single-center study, gene expression levels were evaluated using real-time TaqMan polymerase chain reaction in PIB of kidneys from 72 deceased donors (DDs) and 18 living donors (LDs). Results CASP3 and BAX expression levels were higher, whereas those of BCL2 were lower, in DD than in LD PIB. In biopsies from DD, BCL2 levels were lower in cases with DGF, whereas no differences were observed concerning CASP3 and BAX. The BAX/BCL2 gene expression ratio greater than 2.29 associated with DGF with an odds ratio of 2.00. A multiple regression analysis including data of TLR4 expression in the first day posttransplant PB from a previous study of our group conducted in the same patients revealed a very strong association of the combination of BAX/BCL2 greater than 2.3 in PIB and TLR4 of 0.95 uRE or lesser in PB with the occurrence of DGF, with OR of 120 and positive and negative predictive values of 91% and 92%, respectively. Conclusions The power to predict DGF of the combination of high BAX/BCL2 expression in PIB and low TLR4 expression in the first day posttransplant peripheral blood observed in the present study is extremely high, in comparison to any other marker or combinations of markers so far published in the literature.
Disordered Speech Production in Aphasic and Normal Speakers

Two empirical studies are presented which seek to extend the parallels between disordered speech production in aphasia and in normals. Study 1 compares the rate and distribution of some theoretically interesting error types in a jargon aphasic and a normal error corpus. Study 2 is an investigation of how the error pattern of normal speakers evolves as utterances become more practiced. On the basis of these studies, we offer a hypothesis about the nature of the variation between more and less disordered systems. Our claim, which is developed in the context of spreading activation models of production, is that such variation is tied to the ability of the system to deliver activation to intended units, relative to that of unintended units, within the time required by the task at hand.
The Effect of a Fear-Avoidance–Based Physical Therapy Intervention for Patients With Acute Low Back Pain: Results of a Randomized Clinical Trial

Study Design. A randomized clinical trial with 4-week and 6-month follow-up periods. Objective. To compare the effect of a fear-avoidance–based physical therapy intervention with standard care physical therapy for patients with acute low back pain. Summary of Background Data. The disability reduction strategy of secondary prevention involves providing specific treatment for patients that are likely to have chronic disability from low back pain. Previous studies have indicated that elevated fear-avoidance beliefs are a precursor to chronic disability from low back pain. However, the effectiveness of physical therapy intervention based on a fear-avoidance model is unknown. Methods. Sixty-six consecutive patients referred to physical therapy with low back pain of less than 8 weeks’ duration were randomly assigned to receive fear-avoidance–based physical therapy (n = 34) or standard care physical therapy (n = 32). The intervention period lasted 4 weeks for this study. Disability, pain intensity, and fear-avoidance beliefs measures were recorded before and after treatment. A 6-month follow-up of the same measures was obtained by mail. Results. An intention-to-treat principle (last value forward) was used for data analyses that tested the primary and secondary hypotheses. The prediction of disability at 4 weeks and 6 months after treatment was significantly improved by considering the interaction between the type of treatment and the initial level of fear-avoidance beliefs. Both groups had significant within group improvements for disability and pain intensity. The fear-avoidance treatment group had a significant improvement in fear-avoidance beliefs, and fear-avoidance beliefs about physical activity were significantly lower than the standard care group at 4 weeks and 6 months after treatment. Conclusion. Patients with elevated fear-avoidance beliefs appeared to have less disability from fear-avoidance–based physical therapy when compared to those receiving standard care physical therapy. Patients with lower fear-avoidance beliefs appeared to have more disability from fear-avoidance–based physical therapy, when compared to those receiving standard care physical therapy. In addition, physical therapy supplemented with fear-avoidance–based principles contributed to a positive shift in fear-avoidance beliefs.
Uses of Machine Translation in the Sentiment Analysis of Tweets

This paper reports on the viability of using machine translation (MT) for determining the original sentiment of tweets, when translating tweets made in internationally less used language into more frequently used ones. The results of the study show that it is possible to use MT and sentiment analysis (SA) systems to produce SA results with significant precision.
Comparison of Analytical and Numerical Models for the Optimization of c-Si Solar Cells’ Front Metallization

Progress in metallization techniques and emitter formation technologies needs fast and reliable tools for the optimization of the metallization pattern of crystalline silicon solar cells. We present and compare three models and discuss their validity and accuracy for different emitter sheet, finger and contact resistances, and finger widths. All models yield a similar optimal number of metallization fingers for a 156 mm × 156 mm large solar cell and broad plateaus concerning the dependence of the conversion efficiency on the finger spacing, but they differ concerning the absolute value of the fill factor and the dependence of the open-circuit voltage on the number of fingers.
Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging.

PURPOSE To evaluate the effect of using the ferumoxides-poly-l-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells. MATERIALS AND METHODS PLL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean +/- 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences. RESULTS Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the endosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, endosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7. CONCLUSION Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.
Comparing the Image Quality of a Mobile Flat-Panel Computed Tomography and a Multidetector Computed Tomography: A Phantom Study

ObjectivesThe aim of this study was to compare the image quality of a compact mobile flat-panel computed tomography (FPCT) capable of extremity imaging and a multidetector computed tomography (MDCT) in examinations with the same radiation dose. Material and MethodsImaging with the FPCT was performed with default settings. Monte Carlo simulations were used to calculate equivalent dose settings for the 320-row MDCT. Simulations were based on and validated by dose measurements. Homogeneity, geometric distortion, artifacts, accuracy of Hounsfield values, contrast, and spatial resolution were evaluated in different imaging phantoms. Whitney-Mann U Test and Spearman &rgr; were used for statistical analysis. ResultsHomogeneity reached 2.5% for the FPCT and 0.5% for the MDCT. Hounsfield values were more accurate and contrast to noise ratios were higher for the MDCT than the FPCT (P ⩽ 0.001). The MDCT depicted more rod inserts than the FPCT did. No significant geometric distortion was detected in either modality. The FPCT was more prone to artifacts around Krischner wires with a diameter of 2 mm (P = 0.05-0.001), whereas the MDCT showed a higher amount of artifacts around wires with a diameter of 0.8 mm (P ⩽ 0.001). Spatial resolution was 1 lp/mm (xy), 1.7 lp/mm (z) for the FPCT and 1 lp/mm (xy), less than 1 lp/mm (z) for the MDCT. ConclusionsWe compared a mobile FPCT and a 320-row MDCT by using the same radiation dose for scans. We found the spatial resolution to be higher in the FPCT. Hounsfield units were more accurate and homogeneity and contrast resolution were better in MDCT. The MDCT was also less prone to artifacts from thick Kirschner wires but showed comparably more artifacts around thin wires.
Arctic warming, increasing snow cover and widespread boreal winter cooling

The most up to date consensus from global climate models predicts warming in the Northern Hemisphere (NH) high latitudes to middle latitudes during boreal winter. However, recent trends in observed NH winter surface temperatures diverge from these projections. For the last two decades, large-scale cooling trends have existed instead across large stretches of eastern North America and northern Eurasia. We argue that this unforeseen trend is probably not due to internal variability alone. Instead, evidence suggests that summer and autumn warming trends are concurrent with increases in high-latitude moisture and an increase in Eurasian snow cover, which dynamically induces large-scale wintertime cooling. Understanding this counterintuitive response to radiative warming of the climate system has the potential for improving climate predictions at seasonal and longer timescales.
A Clonal Line of Mesencephalic Progenitor Cells Converted to Dopamine Neurons by Hematopoietic Cytokines: A Source of Cells for Transplantation in Parkinson's Disease

Neural progenitor cells potentially provide a limitless, on-demand source of cells for grafting into patients with Parkinson's disease (PD) if the signals needed to control their conversion into dopamine (DA) neurons could be identified. We have recently shown that cytokines which instruct cell division and differentiation within the hematopoeitic system may provide similar functions in the central nervous system. We have shown that mitotic progenitor cells can be isolated from embryonic rat mesencephalon and that these cells respond to a combination of interleukin-1, interleukin-11, leukemia inhibitory factor, and glial cell line-derived neurotrophic factor yielding a tyrosine hydroxylase-immunoreactive (THir) phenotype in 20-25% of total cells. In the present study, 24 clonal cell lines derived from single cells of mesencephalic proliferation spheres were examined for their response to the cytokine mixture. The clone yielding the highest percentage of THir neurons (98%) was selected for further study. This clone expressed several phenotypic characteristics of DA neurons and expression of Nurr1. The response to cytokines was stable for several passages and after cryopreservation for several months. When grafted into the striatum of DA-depleted rats, these cells attenuated rotational asymmetry to the same extent as freshly harvested embryonic DA neurons. These data demonstrate that mesencephalic progenitor cells can be clonally expanded in culture and differentiated in the presence of hematopoietic cytokines to yield enriched populations of DA neurons. When transplanted, these cells provide significant functional benefit in the rat model of PD.
Quercetin Inhibits Diethylnitrosamine-Induced Hepatic Preneoplastic Lesions in Rats

Quercetin is an antioxidant flavonoid, found ubiquitously in nature and extensively used in herbal medicines and food additives. This study aimed to investigate the effect of quercetin on diethylnitrosamine-induced preneoplastic lesions, using the medium-term rat liver bioassay. The γ-benzene hexachloride was used as promoter at the doses of 0.1, 1.0, and 10.0 mg/kg against a single dose of diethylnitrosamine (200 mg/kg) in male Sprague-Dawley rats. All the rats were subjected to 70% partial hepatectomy at Week 4. The protective effect of quercetin (5 and 25 mg/kg) was examined against the highest dose of γ-benzene hexachloride (10 mg/kg). A significant increase in the number as well as the mean area of glutathione S-transferase placental form (GST-P) positive foci, p53 positive hepatocytes, and the percentage of apoptotic cells were observed in the diethylnitrosamine-treated group. In the present investigation, both doses of QC (5 and 25 mg/kg) led to a significant decrease in the number as well as the mean area of GST-P positive foci, TUNEL positive apoptotic cells, p53 positive hepatocytes, and restoration of cellular morphology. These results clearly indicate that quercetin inhibits diethylnitrosamine-induced hepatic preneoplastic lesions in medium-term rat liver bioassay.
On the properties of time trajectories learned by the cerebellar cortex

The cerebellum has a role in motion control and coordination, but the mechanisms of movement coordination are unknown. Enough physiological data are known on the cerebellar cortex to build a realistic model of its function. The interpretation of Chauvet and Chauvet (1999), hierarchically and mathematically oriented, answers the main issue of the functional relation between the regular, homogeneous observed neural tissue and the high capacity of movement regulation. This approach led to a mathematical definition of the functional unit of the cerebellum based on the stability of its dynamics. Here, we extend the work of Chapeau-Blondeau and Chauvet (1991), to explore the efficiency and the capacity of the cerebellar cortex to learn and memorize trajectories, i.e. time series patterns, taking into account its anatomical features. This is important because the field theory we have conceived involves the whole geometry of the system, thus the role of the delays of propagation between any cells, and because we have not yet any idea of the number of possible learned patterns. Using the most recent anatomical data on the cerebellum, the aim in this paper is: (i) to build an element of the cerebellar cortex using a semi-real neural network and an object-oriented implementation, (ii) to study the role of the most important parameters on the capacity of the cerebellar cortex to learn and retrieve temporal patterns, and (iii) to see to what extent the results of Chapeau-Blondeau and Chauvet may be usable.
Wideband extraction of soil dielectric spectrum from vector-network-analyzer measurements

We present a methodology for soil relative-dielectric-permittivity characterization in the 0.001–3 GHz frequency range. Our approach relies on a vector-network-analyzer scatteringparameter measurement of a soil sample placed into a 1 5/8” coaxial transmission line. In order to calibrate the vector-network-analyzer we use a new calibration technique which combines the multiline through-reflect-line and through-reflect-match schemes, and therefore overcomes the inherent low-frequency limitation of the multiline through-reflect-line approach. We determine the relative dielectric permittivity from sample scattering-parameters by use of a new multi-frequency extraction algorithm which overcomes the low-frequency inaccuracies of non-iterative approaches. Experimental results for soil samples with different moisture content and salinity confirm the validity of our methodology.
Enzyme immobilization by radiation‐induced polymerization of hydrophobic glass‐forming monomers at low temperatures

Enzyme immobilization was studied by means of radiation‐induced polymerization of hydrophobic glass‐forming monomers at low temperatures. The polymerized hydrophobic composite was generally obtained in microspheric form. Enzymatic activity showed little decrease with repeated use in these systems. The particle size of the microsphere increased with increasing monomer concentration, and activity yield had a maximum at an optimum monomer concentration. Immobilization by copolymerization of hydrophilic and hydrophobic comonomers was also investigated and a maximum activity yield was found at a certain monomer concentration. A model scheme for immobilization at low temperatures was proposed and discussed.
Analysis of human urine for pyridine-N-oxide metabolites of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, a tobacco-specific lung carcinogen.

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a potent pulmonary carcinogen in rodents and is believed to be a causative factor for lung cancer in smokers. NNK also may be involved in oral cancer etiology in users of smokeless tobacco products. Pyridine-N-oxidation of NNK and its major metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), produces NNK-N-oxide and NNAL-N-oxide, respectively, which are detoxification products of NNK metabolism and are excreted in the urine of rodents and primates. Our goal is to develop a panel of urinary biomarkers to assess the metabolic activation and detoxification of NNK in humans. In this study, we developed methodology to analyze human urine for NNK-N-oxide and NNAL-N-oxide. The key step in the method was conversion of the N-oxides to NNK and NNAL by treatment with Proteus mirabilis. The resulting samples were then analyzed essentially by methods that we have described previously. 4-(Methylnitrosamino)-4-(3-pyridyl-N-oxide)-1-butanol (iso-NNAL-N-oxide) was used as internal standard. Levels of NNAL-N-oxide in smokers' urine ranged from 0.06 to 1.4 pmol/mg creatinine, mean +/- SD 0.53 +/- 0.36 pmol/mg creatinine. Its presence was confirmed by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry. NNK-N-oxide was not detected in smokers' urine. Levels of NNAL-N-oxide in the urine of smokeless tobacco users ranged from 0.02 to 1.2 pmol/mg creatinine, mean +/- SD 0.41 +/- 0.35 pmol/mg creatinine. The amounts of NNAL-N-oxide in urine were less than 20% of those of [4-(methylnitrosamino)-1-(3-pyridyl)but-1-yl]-beta-O-D-glucosiduronic acid (NNAL-Gluc) and were approximately 50% as great as those of free NNAL. These results demonstrate that pyridine-N-oxidation is a relatively minor detoxification pathway of NNK and NNAL in humans. The method was applied to analysis of urine from 11 smokers who consumed a diet containing watercress. In an earlier study (S.S. Hecht et al., Cancer Epidemiol., Biomarkers & Prev., 4: 877-884, 1995), we showed that consumption of watercress, a source of phenethyl isothiocyanate (PEITC), caused an increase in urinary excretion of NNAL plus NNAL-Gluc. This was attributed to inhibition of alpha-hydroxylation of NNK by PEITC, as seen in rodents in which PEITC also inhibits the pulmonary carcinogenicity of NNK. However, PEITC also could have inhibited pyridine-N-oxidation of NNK and NNAL. The urine of these smokers was analyzed for NNAL-N-oxide. The results demonstrated that watercress consumption had no effect on levels of NNAL-N-oxide in urine, supporting the conclusion that PEITC does inhibit the metabolic activation of NNK in humans.
New relationships of human hematopoietic lineages facilitate detection of multipotent hematopoietic stem and progenitor cells

Three important goals of hematopoietic stem cell research are to understand of how hematopoietic stem cells (HSCs) self-renew, how lineage commitment takes place, and how HSCs can be expanded ex vivo. Research in this area requires a reliable model of hematopoiesis. Performing detailed functional analyses of human hematopoietic progenitor subsets, we recently gained evidence for new hematopoietic lineage relationships.1 According to our data, neutrophils belong to the same branch of the hematopoietic tree as lymphocytes. In contrast, eosinophils and basophils derive from another branch, the erythro-myeloid branch. Here, after introducing the newly proposed hematopoietic model, we discuss its consequences for the identification and expansion of human multipotent progenitors and suggest a fast and reliable method to screen for multipotent hematopoietic cells in vitro.
Rheumatoid factors and secondary immune responses in the mouse II. Incidence, kinetics and induction mechanisms

Production of IgM rheumatoid factor (RF) during secondary immune responses was studied in normal mouse strains by measuring RF synthesis in spleen cell cultures. A considerable, although transient, RF response was observed in BALB/c and C57BL/6 mice immunized and i. v. boosted with various protein antigens and with sheep erythrocytes. With most antigens, RF production equalled or exceeded that of antigen‐specific IgM, but it peaked and subsided earlier. No RF production was detected when either priming or boosting was omitted. The isotypic specificity of RF depended upon the antigens used for immunization: all proteins tested stimulated the synthesis of RF specific for IgG1, whereas sheep erythrocytes induced both IgG1‐ and IgG2a‐specific RF. Reconstitution experiments of irradiated naïve C57BL/6 mice with various combinations of syngeneic antibody against human transferrin and primed or unprimed spleen cells indicated that important RF responses could be induced only when antigen was injected into mice that had received both antibody and immune spleen cells.
Recommended Standards for Fetal Magnetocardiography

Fetal magnetocardiography (FMCG) is increasingly being used in research and diagnostics of fetal heart function. Currently, FMCG is the only noninvasive procedure available, comparable to postnatal ECG, which can be used to assess cardiac electrophysiology during the second and third trimester of pregnancy. For a reliable evaluation and full clinical acceptance of this new technique, large numbers of patient investigations are required which can only be obtained in multicenter studies. An international standard protocol is needed to allow pooling of sufficient data and to permit the comparison of studies performed in different centers. This article provides recommended standards for FMCG in the fields of data acquisition and data analysis. (PACE 2003; 26:2121–2126)