Miracle-dz/UniFAR-FTUs huggingface dataset:
High-throughput profiling and analysis of plant responses over High-throughput profiling and analysis of plant responses over time to abiotic stress time to abiotic stress

Sorghum ( Sorghum bicolor (L.) Moench) is a rapidly growing, high-biomass crop prized for abiotic stress tolerance. However, measuring genotype-by-environment (G x E) interactions remains a progress bottleneck. We subjected a panel of 30 genetically diverse sorghum genotypes to a spectrum of nitrogen deprivation and measured responses using high-throughput phenotyping technology followed by ionomic profiling. Responses were quantified using shape (16 measurable outputs), color (hue and intensity), and ionome (18 elements). We measured the speed at which specific genotypes respond to environmental conditions, in terms of both biomass and color changes, and identified individual genotypes that perform most favorably. With this analysis, we present a novel approach to quantifying color-based stress indicators over time. Additionally, ionomic profiling was conducted as an independent, low-cost, and high-throughput option for characterizing G x E, identifying the elements most affected by either genotype or treatment and suggesting signaling that occurs in response to the environment. This entire dataset and associated scripts are made available through an open-access, user-friendly, web-based interface. In summary, this work provides analysis tools for visualizing and quantifying plant abiotic stress responses over time. These methods can be deployed as a time-efficient method of dissecting the genetic mechanisms used by sorghum to respond to the environment to accelerate crop improvement. Here, we to define a set of measurable, environmentally dependent, phenotypic outputs to aid crop improvement. We utilized automated phenotyping techniques under controlled-environmental conditions to characterize G x E interactions on a diverse panel of sorghum genotypes in response to abiotic stress. Specifically, we describe and quantify statistically robust differences among the genotypes to nutrient-poor conditions using three phenotypic characteristics: biomass, color, and ion accumulation. Using image analysis to characterize leaf color and biomass over time in conjunction with ionomics, we report measurable, genetically encoded, phenotypic traits that are affected by nitrogen treatment. This work presents a foundation for understanding the range of sorghum early responses to abiotic stress and provides tools for analyzing other available datasets.
Web-based research: Methodological variables' effects on Web-based research: Methodological variables' effects on dropout and sample characteristicsWeb-based research: dropout and sample characteristicsWeb-based research: Methodological variables' effects on dropout and sample Methodological variabl

Previous research has made a beginning in addressing the importance of methodological differences in Web-based research. The present paper presents four studies investigating whether sample type, financial incentives, time when personal information is requested, table design, and method of obtaining informed consent influence dropout and sample characteristics (both demographics and measured attitudes). Undergraduates were less likely to drop out than nonstudents, and nonstudents offered a financial incentive were less likely to drop out than those offered no incentive. Complex tables, tables that were too wide, requests for personal information on the first page, and the imposing of additional informed consent procedures each provoked early dropout. As was expected, nonstudents and those presented with complex tables showed more measurement error and attitude differences. Asking for personal information and imposing additional consent procedures affected the demographic makeup, raising challenges to external validity.
A Time Domain Passivity Control Scheme for Bilateral Teleoperation

Conventional time domain passivity control inevitably embodies division. Zero division can occur under a tiny force or velocity, which may be inevitable, and will be the cause of control crash. To avoid the zero division problem and control crash, we propose a switching dissipation controller for guaranteed stability. The parametric design of the proposed approach is discussed. The switching time domain passivity control is then applied to teleoperation and safe operation is achieved. Simulation and experimental results are demonstrated to validate the effectiveness of the proposed control scheme.
Edinburgh Research Explorer Reclaiming their lives

Objectives : Adult-to-adult living liver donation (LLD) is a controversial procedure due to the risk to the healthy donor. The decision to proceed with LLD is an important, yet under-researched area. This study aims to explore the decision-making process of the donor and recipient independently, and within the donor – recipient dyad. Design : A longitudinal, qualitative analysis of the LLD decision from the perspective of a LLD donor – recipient dyad. Methods : In-depth interviews were conducted with the donor and recipient separately on three occasions: pre LLD, six weeks post and six months post LLD. Transcripts were subject to interpretative phenomenological analysis. Results : During the pre-LLD interviews, a series of intra- and interpersonal negotiations were reported as both the donor and recipient grappled to make a decision about LLD. Following the decision, the focus then centred on the consequences of the decision and making sense of unanticipated outcomes. By six months post LLD, both were able to re ﬂ ect on adapting to the changes their decision had ultimately caused. Conclusions : This case study offers a unique insight into the risk assessment and decision-making demands of LLD and the results can help support future LLD candidates.
i dentification f actors i nfluencing e- learning s atisfaction during covid-19 p andeMic p eriod aMong s tudents at a M alaysia p rivate i nstitution

The rapid occurrences of Corona Virus Disease (Covid-19) have posed various challenges to our society, including the quality of education system in Malaysia. The current study aims to first measure the impact of demographic variables (gender, age and education level) and non-demographic variables (perceived usefulness, perceived ease of use and technological factor) on e-learning satisfaction among students from a higher education institution in Malaysia, and to construct a student satisfaction prediction model subsequently. A structured questionnaire survey was administered to a total of 344 respondents and the statistical analysis of Chi-Square test and Multiple Regressions was performed. The result of the study reveals that satisfaction of students on e-learning experience during the pandemic is significantly corelated to gender. The study further shows that three dominant variables, namely perceived usefulness, perceived ease of use and technological factor are significant factors that influence the satisfaction level of students’ learning in the e-learning environment. The findings of the study will be beneficial to policy makers to identify the level of student satisfaction on e-learning education during for this study was to analyse the factor s that affected student s’ satisfaction on e - learning during the COVID - 19 pandemic in Malaysia . The study adapted Davis’s (1989) Technology Adoption Model (TAM) to measure the variables using a Likert scale of 1 – 5 with end points of ‘strongly disagree’ and ‘strongly agree’ . T his instrument is also widely used by researchers for similar study in other contexts (DeLone & McLean, 2003; Pilli, Fanaeian &Al -Momani, 2014; Mohammadi, 2015).
DEVELOPING A VALUE MODEL OF WELLNESS SERVICE

The aim of this study was to develop a value model of wellness service. In this study, those persons who work in health care systems were recruited to participate in interviews for investigating the value of various roles and the effects of these values between the roles. Throughout this study, the flow model, sequence model and cultural model were established to explain the relationship between the different roles, the process being influenced by others, and the effects of other values in wellness-related activities. According to the models, the following features of value in wellness activities could be found: personal, interactive and temporal. Based on the result of this study, the investigating and presenting the relevance of effects between values more completely will be researched in the next study.
AntigenBiomarker Midkine Is a Tumor-Shared The Angiogenic Growth Factor and

The angiogenic factor Midkine (MDK) is overexpressed in various human malignant tumors, although its expression is low or undetectable in normal adult tissues. Its expression in tumors and its detection in plasma have been associated with poor disease outcome, whereas its blockade was found to contribute to tumor regression. By weekly stimulation of T lymphocytes harvested in HLA-A2 healthy donors, we derived CD8 T cell lines speciﬁc for several MDK peptides. The T cell response was mostly dominated by two nonamer peptides localized in the signal peptide and in the C-terminal part of the protein, as assessed by IFN- g ELISPOT and HLA-A2 tetramer labeling. Peptide-speciﬁc T cell lines recognized cells transfected with an MDK-encoded plasmid and tumor cell lines naturally expressing the MDK protein, but not untransfected cells. T cell presentation of the two MDK epitopes was found to be TAP dependent. Experiments performed in HLA-A2 transgenic mice demonstrated the capacity of the two identiﬁed CD8 T cell epitopes to elicit a cytotoxic response. Altogether, our data show that the secreted MDK protein is a candidate vaccine for multiple cancers. The Journal of Immunology , 2010, 185: 000–000.
Modeling and Optimization for Stationary Base Engine Calibration

This thesis presents new approaches for modeling and optimization for stationary base engine calibration. At first, the most suitable type of modeling for this topic is determined in an extensive comparison. Afterwards, this technique is extended by new features, e.g. outlier-robustness, in order to achieve a dependable performance of the model even under complex and difficult circumstances. Subsequently, a new model-based multi-objective online optimization is presented, which is able to automatically identify the Pareto-optimal areas of the combustion engine with a permanent online-connection between the optimization algorithms and the test bench. Various theoretical examples and practical applications demonstrate the performance of these new approaches.
STARDUST AND STATISTICS Situated language and literacy in Pokémon Go guides

The ability to understand language-in-use is essential to language and literacy learning. This article focuses on players' acquisition of specialist language and Discourses (Gee, 2014) among players of the mobile augmented reality game Pokémon GO. Specifically, I explore player-written guides by self-identified researchers of the game. These researchers enact scientific Discourses to explain gameplay elements. Using parent interviews and excerpts from the player-written guides, I analyze these game guides' highly specialized and complex language as forms of situated language in-use. I conclude with a discussion of impli- cations based on these findings for L1 in educational settings.
Sentiment Diffusion of Social Inequality in Microblogs: A Case Study of "Migrant Worker" in Sina Weibo

: Migrant workers constitute the main city workforce in China. However, they are the victims of social inequality. Sina Weibo is one of the most important channels for people to share information and public opinions. In order to study into the sentiment diffusion of social inequality over Sina Weibo, we collected a huge number of root microblogs and reposts based on the search query “Migrant Worker”. With applying the sentiment tendency analysis tool provided by Baidu AI, we were able to capture the sentiment flipping process. We found that most microblog users tended to follow the previous users’ sentiment polarity. But the intensity of the sentiment polarity would always get weaken.
Razvoj laboratorijskog sistema za merenje karakteristika fotonaponsko-termičk og panela

The emissions of harmful gases into the atmosphere, air pollution and global warming can be greatly reduced by increasing the use of renewable energy sources such as solar energy to heat domestic hot water, space heating and generating electricity. This paper describes the systems of combined (hybrid) PV/T panels that can simultaneously generate both heat and electricity, as well as the characteristics of the PV/T panel. A prototype of the laboratory setting for the examination of these systems along with the used components is also shown. The temperature, fluid flow rate values, obtained heat and electric gain are also presented. Deficiencies and proposed changes in the laboratory setup and used equipment were laid out, in order to improve the accuracy of the measurements.
O PPORTUNITIES FOR A CTIVE S TOCK -O UT M ANAGEMENT IN O NLINE S TORES : T HE I MPACT OF THE S TOCK -O UT P OLICY ON O NLINE S TOCK -O UT R EACTIONS

This paper investigates the impact of an online retailer’s stock-out policy on consumers’ category purchase and choice decisions. We investigate three different policies: (1) stock-outs are immediately visible and there are no replacement suggestions , (2) stock-outs are only visible after purchase attempts and (3) stock-outs are immediately visible but a replacement item is suggested . Results from an online grocery shopping experiment reveal that the adopted stock-out policy has a significant impact on both decisions. Making stock-outs not immediately visible creates confusion and intensifies the consumer’s loss experience, thereby reducing the tendency to buy in the category. Suggesting a replacement item, in contrast, facilitates the substitution decision and slightly reduces the purchase cancellation rate. It also substantially increases the suggested item’s choice probability. Yet, this effect disappears when higher-priced – suspicious – items are suggested. Overall, these results indicate that online grocery retailers have an interest in pursuing open and convenience-oriented stock-out policies.
3 - A database for microarray data

High-throughput gene expression technologies generate a large amount of data. However, although many data sets are readily available on the Internet, it is hard even for specialists to collect, standardize and use them. For the bench biologist, these data are available in theory, but are not used in practice. Publicly available data are scattered on different Web sites, and now databases. This system presents many inconveniences: the sites are not always as stable as they should be and the results are presented in a non-standard form (plain text, or HTML, or presented as a database,.), sometimes indexed by gene, sometimes by EST (Image ID or Genbank ID or.) and finally the numerical data are either non-normalized or normalized in a certain, and often unique, way. Because of this, extracting an information of interest in these data is a daunting task. In order to make gene expression information intelligible, we, and other (Aach et al., 2001) have developed databases, allowing to store and retrieve those data. Moreover, a biologist-friendly web-based interface allowing querying the data was created.
Institutional Knowledge at Singapore Management University Institutional Knowledge at Singapore Management University

Programming heavily relies on entering text using traditional QWERTY keyboards, which poses challenges for people with limited upper-body movement. Developing tools using a publicly available speech recognition API could provide a basis for keyboard free programming. In this paper, we describe our efforts in design, development, and evaluation of a voice-based IDE to support people with limited dexterity. We report on a formative Wizard of Oz (WOz) based design process to gain an understanding of how people would use and what they expect from a speech-based programming environment. Informed by the findings from the WOz, we developed VocalIDE, a prototype speech-based IDE with features such as Context Color Editing that facilitates vocal programming. Finally, we evaluate the utility of VocalIDE with 8 participants who have upper limb motor impairments. The study showed that VocalIDE significantly improves the participants’ ability to make navigational edits and select text while programming.
NonspecificImmunoregulatory T Cells Is Antigen + CD25+ Suppressor Effector Function of CD4

1 CD4 1 CD25 1 T cells represent a unique population of “professional” suppressor T cells that prevent induction of organ-speciﬁc autoimmune disease. In vitro, CD4 1 CD25 1 cells were anergic to simulation via the TCR and when cultured with CD4 1 CD25 2 cells, markedly suppressed polyclonal T cell proliferation by speciﬁcally inhibiting the production of IL-2. Suppression was cytokine independent, cell contact dependent, and required activation of the suppressors via their TCR. Further characterization of the CD4 1 CD25 1 population demonstrated that they do not contain memory or activated T cells and that they act through an APC-independent mechanism. CD4 1 CD25 1 T cells isolated from TCR transgenic (Tg) mice inhibited responses of CD4 1 CD25 2 Tg T cells to the same Ag, but also inhibited the Ag-speciﬁc responses of Tg cells speciﬁc for a distinct Ag. Suppression required that both peptide/MHC complexes be present in the same culture, but the Ags could be presented by two distinct populations of APC. When CD4 1 CD25 1 T cells were cultured with anti-CD3 and IL-2, they expanded, remained anergic, and in the absence of restimulation via their TCR, suppressed Ag-speciﬁc responses of CD4 1 CD25 2 T cells from multiple TCR transgenics. Collectively, these data demonstrate that CD4 1 CD25 1 T cells require activation via their TCR to become suppressive, but once activated, their suppressor effector function is completely nonspeciﬁc. The cell surface molecules involved in this T-T interaction remain to be characterized. The Journal of Immunology, 2000, 164: 183–190.
PE, now’: Understanding care-experienced young people’s (dis)engagement with physical education

Young people’s experiences of, and (dis)engagement with, physical education has received considerable attention in recent years. Yet one ‘group’, care-experienced young people , remain ‘hidden’ within the prevailing literature. In light of their apparent invisibility within research, this novel, exploratory study seeks to gain some understanding of the factors associated with (dis)engagement from/with physical education among this youth population. In contrast to the few studies that explore the broader physical culture experiences of care-experienced youth that prioritise the voices of adults, this paper combines data from two studies to give voice to the experiences of four care-experienced young men in England, alongside those of key adults, namely residential staff, foster carers and physical education teachers. Data were derived from participatory research methods with the young people and semi-structured interviews with the adults who work with/for them. Drawing upon Bourdieu, principally his notions of field, habitus and capital, the findings suggest that these care-experienced young people are at a pedagogic disadvantage, since they are not as well positioned to access opportunities for learning and participation or develop, maintain and extend those skills and dispositions that are recognised as valued capital in physical education. Moreover, the changing room, as a sub-field of the broader physical education space, where bodies are particularly on display, may present obstacles for care-experienced young people’s engagement due to their prior experiences of physical and/or sexual abuse. This study therefore calls for further research exploring care-experienced young people’s experiences of physical education, teachers’ understandings of care-experienced youth, and how their pedagogic practice might shape (dis)engagement with physical education.
Long Non-coding RNA snaR Promotes Proliferation in EGFR Wild Type Non-Small Cell Lung Cancer Cells

Lung cancer is the second most common cancer and has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Over-expression of epidermal growth factor receptor (EGFR) has been clarified in different cancers, and has been shown to have a crucial role in tumor progression. In this study, we evaluated long non-coding RNA small NF90-associated RNA (snaR) expression in different EGFR-statue cell lines. Knockdown experiments were conducted to analyze snaR expression in selected cell lines. MTT and transwell assays were respectively employed to evaluate the proliferative and invasive abilities of NSCLC cells. The expression of snaR was remarkably up-regulated in SPC-A1 and A549 wild-type EGFR cell lines. Down regulation of snaR with small interfering RNA significantly inhibited cell invasion as well as proliferation of SPC-A1 and A549 cells. Our results indicate that snaR may be a potential therapeutic biomarker for NSCLC.
Two Endosomal NHX-type + Antiporters are Involved in Auxin Mediated Development in

51 In Arabidopsis thaliana , the endosomal localised Na + /H + antiporters NHX5 and NHX6 regulate ion and 52 pH homeostasis and are important for plant growth and development. However, the mechanism of how 53 these endosomal NHXs function in plant development is not well understood. Auxin modulates plant 54 growth and development through the formation of concentration gradients in plant tissue to control cell 55 division and expansion. Here, we identified a role for NHX5 and NHX6 in the establishment and 56 maintenance of auxin gradients in embryo and root tissues. We observed developmental impairment 57 and abnormal cell division in embryo and root tissues in the double knockout nhx5 nhx6 , consistent with 58 these tissues showing high expression of NHX5 and NHX6 . Through confocal microscopy imaging with 59 the DR5::GFP auxin reporter, we identify defects to the perception, accumulation, and redistribution of 60 auxin in nhx5 nhx6 cells. Furthermore, we find that the steady state levels of the PIN-FORMED (PIN) 61 auxin efflux carriers PIN1 and PIN2 are reduced in nhx5 nhx6 root cells. Our results demonstrate that 62 NHX5 and NHX6 function in auxin mediated plant development by maintaining PIN abundance at the 63 plasma membrane, and provides new insight into the regulation of plant development by endosomal 64 NHX antiporters. cleared embryos revealed low penetrance cell patterning and heart stages of development in nhx5 nhx6 2). Defects were apparent in the basal cell region corresponding to the embryonic root, along with cell patterning anomalies in the tips of the incipient cotyledons. Together with primary and lateral root data, these findings indicate that NHX5 and NHX6 function in both embryo and root growth and development, two tissues that are critically