Miracle-dz/UniFAR-FTUs huggingface dataset:
Lipid extraction has tissue‐dependent effects on isotopic values (δ34S, δ13C, and δ15N) from different marine predators

Rationale The use of sulfur isotopes to study trophic ecology in marine ecosystems has increased in the past decade. Unlike other commonly used isotopes (e.g., carbon), sulfur can better discriminate benthic and pelagic productivity. However, how lipid extraction affects sulfur isotopic values has not been assessed, despite its frequent use to remove lipid effects on δ13C values. Methods We used white muscle and liver samples from two species of sharks and skin samples from two species of pinnipeds (sea lion and fur seal) to assess the effects of lipid extraction on stable isotope values for δ34S, δ13C, and δ15N. Isotopic values were determined using a continuous flow‐isotope ratio mass spectrometer coupled to an elemental analyzer. Results Lipid extraction significantly decreased δ34S values in shark tissues, more so for liver than muscle (−4.6 ± 0.9‰ vs −0.8 ± 0.3‰, average change), with nearly no change in their standard deviations. Lipid extraction did not affect δ34S values from pinniped skin samples (0.2 ± 0.8‰, average change). After lipid extraction, consistent increases in δ13C values (0.2‰–7.3‰) were detected as expected, especially in tissue with high lipid content (C:N >4). After lipid extraction, significant increases in δ15N values (0.5‰–1.4‰) were found in shark muscle and liver tissues. For pinniped skin samples, δ15N values were not significantly lower after lipid extraction (−0.4‰ to –0.1‰). Conclusions Lipid extraction did not have a strong impact on δ34S values of shark muscle and pinniped skin (≤1‰). However, our results suggest it is essential to consider the effects of lipid extraction when interpreting results from δ34S values of shark liver tissue, as they significantly depleted values relative to bulk tissue (~5‰). This may reflect selective removal of sulfolipids and glutathione present in higher concentrations in the liver than in muscle and skin and requires further investigation.
Exogenous Hemin alleviates cadmium stress in maize by enhancing sucrose and nitrogen metabolism and regulating endogenous hormones

Abstract Cadmium (Cd) stress restricts maize growth and productivity severely. We aimed to investigate the effects of Hemin on the metabolism of sucrose and nitrogen and endogenous hormones in maize under cadmium stress. Maize varieties ‘Tiannong 9’ (cadmium tolerant) and ‘Fenghe 6’ (cadmium sensitive) were grown in nutrient solutions to study the effects of Hemin on maize physiological and ecological mechanisms under cadmium stress. The results showed that Hemin mediated the increase of sucrose content and the activities of key enzymes sucrose phosphate synthase (SPS) and sucrose synthase (SS) in maize leaves under cadmium stress. Soluble acid invertase (SAInv) and basic/neutral invertase (A/N-Inv) enzyme activities in leaves were decreased significantly, and sucrose accumulation in leaves was increased. Hemin also mediated the increase of NO3 − content in leaves, the decrease of NH4 + content and the increase of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase activity (GOGAT) and glutamate dehydrogenase (GDH) enzyme activities under cadmium stress. The contents of IAA, ZR, and GA in leaves and roots increased, ABA, MeJA, and SA decreased, and IAA/ABA, ZR/ABA, and GA/ABA increased under cadmium stress. Our study showed Hemin can alleviate cadmium stress in maize by enhancing sucrose and nitrogen metabolism and regulating endogenous hormones. NOVELTY STATEMENT This work further investigates the effects of Hemin on the metabolism of sucrose and nitrogen and endogenous hormones in maize under cadmium stress, which, hopefully, is to guide Hemin application to maize field resilience production. It also explains that Hemin is beneficial for dry matter accumulation and transport, alleviated ammonia toxicity and nitrogen metabolism disorder, and induced the changes of endogenous hormone content and the adaptive hormone ratio balance under cadmium stress.
Gallocatechin-silver nanoparticles embedded in cotton gauze patches accelerated wound healing in diabetic rats by promoting proliferation and inhibiting apoptosis through the Wnt/β-catenin signaling pathway

Background: Diabetes mellitus is a chronic metabolic disorder characterized by elevated plasma glucose levels. It is often defined as a lifestyle disease having severe economic and physiological repercussions on the individual. One of the most prevalent clinical consequences of diabetes is the lagging pace of injury healing leading to chronic wounds, which still to date have limited treatment options. The objective of this research is to look into the wound healing capabilities of gallocatechin (GC) and silver nanoparticles (AgNPs) impregnated patches in diabetic rats. Experimental rats were dressed patches and the wound healing skin region was dissected at the end of the experiment for molecular analysis. The wound healing rate in diabetic rats dressed with CGP2 and CGP3 & silver sulfadiazine (AgS) patches were found to be high. While mRNA and immunofluorescence or immunohistochemistry assays reveal that Wnt3a and β-catenin levels were higher with Gsk-3β and c-fos levels were lower in diabetic rats dressed with in CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. Furthermore, apoptosis markers such as caspase-3, caspase-9, and Bax levels were reduced, whereas anti-apoptosis maker (Bcl-2) and proliferation marker (PCNA) levels were increased in diabetic rats dressed with CGP2 and CGP3 as compared with diabetic rats dressed with DC+CGP1. In conclusion, the results demonstrated that GC-AgNPs-CGP (CGP2 & CGP3) dressing on diabetes wound rats decreased changes in Wnt3a/β-catenin pathways, resulting in lower apoptosis and greater proliferation, so drastically improving diabetic wound healing.
Self-supervised learning for multi-center magnetic resonance imaging harmonization without traveling phantoms

Objective. With the progress of artificial intelligence (AI) in magnetic resonance imaging (MRI), large-scale multi-center MRI datasets have a great influence on diagnosis accuracy and model performance. However, multi-center images are highly variable due to the variety of scanners or scanning parameters in use, which has a negative effect on the generality of AI-based diagnosis models. To address this problem, we propose a self-supervised harmonization (SSH) method. Approach. Mapping the style of images between centers allows harmonization without traveling phantoms to be formalized as an unpaired image-to-image translation problem between two domains. The mapping is a two-stage transform, consisting of a modified cycle generative adversarial network (cycleGAN) for style transfer and a histogram matching module for structure fidelity. The proposed algorithm is demonstrated using female pelvic MRI images from two 3 T systems and compared with three state-of-the-art methods and one conventional method. In the absence of traveling phantoms, we evaluate harmonization from three perspectives: image fidelity, ability to remove inter-center differences, and influence on the downstream model. Main results. The improved image sharpness and structure fidelity are observed using the proposed harmonization pipeline. It largely decreases the number of features with a significant difference between two systems (from 64 to 45, lower than dualGAN: 57, cycleGAN: 59, ComBat: 64, and CLAHE: 54). In the downstream cervical cancer classification, it yields an area under the receiver operating characteristic curve of 0.894 (higher than dualGAN: 0.828, cycleGAN: 0.812, ComBat: 0.685, and CLAHE: 0.770). Significance. Our SSH method yields superior generality of downstream cervical cancer classification models by significantly decreasing the difference in radiomics features, and it achieves greater image fidelity.
Validation of the Turkish version of the DOSE‐Nonadherence measure among patients with cardiometabolic conditions

There are no validated self‐report measures to assess extent of and reasons for medication nonadherence in the Turkish language. The aim of this study is to evaluate validity and reliability of the Domains of Subjective Extent of Nonadherence Scale, which assesses extent of and reasons for nonadherence in Turkish patients with hypertension, diabetes mellitus and/or dyslipidaemia in community pharmacy settings.
Inflammatory markers may predict post‐operative complications and recurrence in Crohn's disease patients undergoing gastrointestinal surgery

Most Crohn's Disease (CD) patients will require surgical intervention over their lifetime, with considerably high rates of post‐operative complications. Risk stratification with reliable prognostic tools may facilitate clinical decision making in these patients. Blood cell interaction based inflammatory markers have proven useful in predicting patient outcomes in oncological and benign diseases. The aim of this study was to investigate their prognostic value in CD patients undergoing surgery.
Novel luciferase‐based glucagon‐like peptide 1 reporter assay reveals naturally occurring secretagogues

Glucagon‐like peptide 1 (GLP‐1) is a hormone derived from preproglucagon. It is secreted by enteroendocrine cells in response to feeding and, in turn, acts as a critical regulator of insulin release. Modulating GLP‐1 secretion holds promise as a strategy for controlling blood glucose levels.
The association between gender, sexual harassment, and self-compassion on depressive symptoms in adolescents

Abstract Aim The study aims to identify the prevalence of bullying and sexual harassment as well as possible risk and protective factors for depression among adolescents. We focus on the association of different types of sexual harassment, gender, bullying, and self-compassion to depression criteria according to DSM 4. Methods This cross-sectional study included 318 adolescents aged 15–20 years, from Sweden. Participants answered the self-assessment scales Reynolds Adolescent Depression Scale, Second Edition, and Compassionate Engagement and Action Scales for Youth, as well as questions about sexual harassment and bullying. Regression analyses were conducted to examine the association between bullying, sexual harassment, self-compassion, and gender with depressive symptoms. Results Few students had been subjected to bullying, whereas sexual harassment was more common. About 32.7% of students had been subjected to verbal harassment and girls were more frequently exposed. Both bullying and sexual harassment were associated with depressive symptoms, and gender patterns were observed. For boys, verbal harassment and bullying correlated with negative self-evaluation and somatic complaints. For girls, bullying correlated with all depressive symptoms. Higher levels of self-compassion were associated with less depression, and the correlation was especially strong among boys. Conclusion Boys and girls express different types of depressive symptoms when subjected to bullying and sexual harassment. Not asking boys about negative self-evaluation or somatic complaints could lead to missing depression. However, for girls, all four symptoms are equally important to inquire about. Self-compassion is a possible protective factor against depression and future studies will show if teaching this to adolescents could lead to less depression.
HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1

Abstract Aha1 is a co-chaperone of heat shock protein 90 (HSP90), and it stimulates the ATPase activity of HSP90 to promote the folding of its client proteins. By employing ascorbate peroxidase (APEX)-based proximity labeling and proteomic analysis, we identified over 30 proteins exhibiting diminished abundances in the proximity proteome of HSP90 in HEK293T cells upon genetic depletion of Aha1. Dicer1 is a top-ranked protein, and we confirmed its interactions with HSP90 and Aha1 by immunoprecipitation followed by western blot analysis. Genetic depletion of Aha1 and pharmacological inhibition of HSP90 both led to reduced levels of Dicer1 protein. Additionally, HSP90 and Aha1 bind preferentially to newly translated Dicer1. Reconstitution of Aha1-depleted cells with wild-type Aha1 substantially rescued Dicer1 protein level, and a lower level of restoration was observed for complementation with the HSP90-binding-defective Aha1-E67K, whereas an Aha1 mutant lacking the first 20 amino acids—which abolishes its chaperone activity—failed to rescue Dicer1 protein level. Moreover, knockdown of Aha1 and inhibition of HSP90 led to diminished levels of mature microRNAs (miRNAs), but not their corresponding primary miRNAs. Together, we uncovered a novel mechanism of HSP90 and Aha1 in regulating the miRNA pathway through promoting the folding of Dicer1 protein, and we also demonstrated that Aha1 modulates this process by acting as an autonomous chaperone and a co-chaperone for HSP90.
Occupational Balance and Depressive Symptoms During the COVID-19 Pandemic

Understanding how daily activities (parenting, caregiving, work, sports, social contact, volunteering) might interplay with the COVID-19 pandemic can help to better allocate resources on segments of the population that are more at risk for mental health problems. This can apply to the current crisis as well as future similar scenarios. Objective The aim of this study was to investigate the relationship among daily activities (paid work, childcare, caregiving, voluntary work, sports, and social contact), occupational balance, and depressive symptoms during the COVID-19 pandemic. Methods We analyzed data from the Austrian Corona Panel Project (four time points, 6-month period) using regression models with logarithmically transformed data and nonparametric repeated-measures tests (N = 871). Results Results showed higher depressive symptoms among women. Family caregivers (either parents or those caring for other relatives) were at the highest risk for occupational imbalance and depressive symptoms. Sports and social contact were initially associated with better outcomes, but the effects waned. There was a main effect for time point driven by the last wave (amidst the second lockdown), but no significant interaction effects between predictors and time point were found. Conclusion The results provide a nuanced depiction of the relationship between different daily activities and health-related outcomes during the pandemic, highlighting groups at risk.
Disproof of Concept: Resolving Ethical Dilemmas Using Algorithms

Ballantyne, A. , and G. O. Schaefer. 2020. Public interest in health data research: Laying out the conceptual groundwork. Journal of Medical Ethics 46 (9):610–6. doi:10.1136/ medethics-2020-106152. COE 2017. Council of Europe, Consultative Committee of the Convention for the protection of individuals with regard to automatic processing of personal data (T-PD), Guidelines on the protection of individuals with regard to the processing of personal data in a world of Big Data, Strasbourg, 23 January 2017,T-PD(2017)01. EC 2020. European Commission (EC), White Paper on Artificial Intelligence A European approach to excellence and trust, Brussels, 19.2. 2020 COM(2020) 65 final. EC 2021. European Commission (EC), DG Health and Food Safety, Assessment of the EU Member States’ rules on health data in the light of GDPR, Specific Contract No SC 2019 70 02 in the context of the Single Framework Contract Chafea/2018/Health/03, Publications Office of the European Union, 2021. doi:10.2818/546193. EDPS 2016. European Data Protection Supervisor (EDPS), Opinion 8/2016, EDPS Opinion on coherent enforcement of fundamental rights in the age of big data, 23 September 2016. European Union Agency for Fundamental Rights and Council of Europe 2018. European Union Agency for Fundamental Rights and Council of Europe. Handbook on European data protection law (2018 edition). Publications Office of the European Union, 2018. doi:10. 2811/343461. Mostert, M. ,. A. L. Bredenoord, B. van der Slootb, and J. J. M. van Delden. 2018. From privacy to data protection in the EU: Implications for Big Data health research. European Journal of Health Law 25 (1):43–55. doi:10. 1163/15718093-12460346. Pyrrho, M. , L. Cambraia, and V. F. de Vasconcelos. 2022. Privacy and health practices in the digital age. The American Journal of Bioethics 22 (7):50–59. doi:10.1080/ 15265161.2022. 2040648. Smuha, N. A. , E. Ahmed-Rengers, A. Harkens, W. Li, J. MacLaren, R. Piselli, and K. Yeung, How the EU Can Achieve Legally Trustworthy AI: A Response to the European Commission’ Proposal for an Artificial Intelligence Act (August 5, 2021). Available at SSRN: https://ssrn.com/abstract=3899991 or http://dx.doi. org/10. 2139/ssrn.3899991 Street, J., B. Fabrianesi, C. Adams, F. Flack, M. Smith, S. M. Carter, S. Lybrand, A. Brown, S. Joyner, J. Mullan, et al. 2021. Sharing administrative health data with private industry: A report on two citizens’ juries. Health Expectations: An International Journal of Public Participation in Health Care and Health Policy 24 (4): 1337–48. doi:10.1111/hex.13268.
β2‐Adrenoceptor agonist profiling reveals biased signalling phenotypes for the β2‐adrenoceptor with possible implications for the treatment of asthma

β‐Adrenoceptor agonists relieve airflow obstruction by activating β2‐adrenoceptors, which are G protein‐coupled receptors (GPCRs) expressed on human airway smooth muscle (HASM) cells. The currently available β‐adrenoceptor agonists are balanced agonists, however, and signal through both the stimulatory G protein (Gs)‐ and β‐arrestin‐mediated pathways. While Gs signalling is beneficial and promotes HASM relaxation, β‐arrestin activation is associated with reduced Gs efficacy. In this context, biased ligands that selectively promote β2‐adrenoceptor coupling to Gs signalling represent a promising strategy to treat asthma. Here, we examined several β‐adrenoceptor agonists to identify Gs‐biased ligands devoid of β‐arrestin‐mediated effects.
Activation of cytotoxic T lymphocytes by self-differentiated myeloid-derived dendritic cells for killing breast cancer cells expressing folate receptor alpha protein

ABSTRACT Adoptive cell transfer (ACT) is a promising approach for cancer treatment. Activation of T lymphocytes by self-differentiated myeloid-derived antigen-presenting-cells reactive against tumor (SmartDC) resulted in specific anti-cancer function. Folate receptor alpha (FRα) is highly expressed in breast cancer (BC) cells and thus potential to be a target antigen for ACT. To explore the SmartDC technology for treatment of BC, we create SmartDC expressing FRα antigen (SmartDC-FRα) for activation of FRα-specific T lymphocytes. Human primary monocytes were transduced with lentiviruses containing tri-cistronic complementary DNA sequences encoding granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), and FRα to generate SmartDC-FRα. Autologous T lymphocytes were activated by SmartDC-FRα by coculture. The activated T lymphocytes exhibited enhanced cytotoxicity against FRα-expressing BC cell cultures. Up to 84.9 ± 6.2% of MDA-MB-231 and 89.7 ± 1.9% of MCF-7 BC cell lines were specifically lysed at an effector-to-target ratio of 20:1. The cytotoxicity of T lymphocytes activated by SmartDC-FRα was also demonstrated in three-dimensional (3D) spheroid culture of FRα-expressing BC cells marked by size reduction and spheroid disruption. This study thus portray the potential development of T lymphocytes activated by SmartDC-FRα as ACT in FRα-expressing BC treatment. Graphical abstract
Family involvement in nursing homes: an interpretative synthesis of literature

Background Family involvement in nursing homes is generally recognized as highly valuable for residents, staff and family members. However, family involvement continues to be challenging in practice. Aim To contribute to the dialogue about family involvement and develop strategies to improve family involvement in the nursing home. Methods This interpretative synthesis consists of a thematic analysis and care ethical interpretation of issues regarding family involvement from the perspective of families in nursing homes reported in literature. Findings This study reveals the complexities of family involvement in the nursing home by drawing attention to the moral dimension of the issues experienced by families, as seen through the theoretical lens of Baier’s care ethical concept of trust as a theoretical lens. The synthesis of literature resulted in a thematic categorization of issues reported by families, namely, family–staff relationship, psychosocial factors and organizational circumstances. The care ethical interpretation of the synthesis of literature showed that the concept of trust resonates with all reported issues. Trust evolves over time. Early issues are mostly related to getting to know each other. Secondly, families want to experience that staff are competent and of good will. Difficult feelings families may have, such as guilt or loneliness, and dealing with the deterioration of the loved one puts families in a vulnerable position. This power imbalance between family and staff impedes a trusting relationship. Issues related to organizational circumstances, such as understaffing, also undermine families' trust in staff and the nursing home. Discussion and conclusion Baier’s theoretical concept of trust provides a deeper insight into the moral dimension of family involvement from the perspective of families in the nursing home. To improve family involvement in practice, we propose to aim future interventions at reinforcing trust in the relationship between family and staff as well as in the organizational context in which these care relationships occur.
Outcomes of Coronary Artery By-Pass Grafting Under Dual Antiplatelet Therapy in ST Elevated Myocardial Infarction

Patients with recent myocardial infarction (MI) or percutaneous coronary interventions (PCI) have a higher ischemic risk in addition to perioperative bleeding risk when undergoing coronary artery bypass grafting (CABG). Data regarding preoperative dual antiplatelet therapy (DAPT) failed to create a clear recommendation. In the present study, we assessed the relationship between preoperative DAPT use and adverse outcomes, particularly in ST-elevation MI (STEMI) patients. We retrospectively analyzed 748 consecutive patients with STEMI who underwent subsequent CABG surgery. Patients were divided into 2 groups: those on DAPT up to the day before CABG and those discontinued DAPT >5 days before CABG. Predictors of in-hospital mortality and major bleeding were analyzed by multivariate analysis. Preoperative DAPT was not associated with in-hospital mortality (Odds Ratio (OR):1.81; 95% Confidence Interval (CI): . 89-3.68, P = . 10) and major bleeding (OR: 1.15; 95% CI: .63-2.08, P = .65) after multivariate analysis. However, glycoprotein (Gp) 2b/3a inhibitors were independently associated with higher major bleeding rates. Age, shock, and EF (ejection fraction) <30% were associated with in-hospital mortality. Previous MI, Gp 2b/3a inhibitors, and EF <30% were predictors of major bleeding. In conclusion, there were no association between pre-CABG DAPT use and in-hospital mortality and major bleeding.
Big Five Personality Traits, Social Networks, and Depression Among Older Adults in Japan: A Multiple Mediation Analysis

This study examines the mediating effect of social network size on depression among older adults in Japan in association with the Big Five personality traits: extraversion, agreeableness, conscientiousness, neuroticism, and openness. Cross-sectional data were obtained from 739 older adults (Mean age = 75.13, SD = 6.86) residing in Tokyo. Multiple mediation analyses estimated total, indirect, and direct effects between personality and depression. Extraversion was associated with the number of kin and friend networks and agreeableness with the number of kin networks. Moreover, these social networks partially mediated the effects of extraversion and agreeableness on depression. The findings show that personality traits such as extraversion and agreeableness are associated with social network size, which contributes to better mental health in old age.