Miracle-dz/UniFAR-FTUs huggingface dataset:
The role of neuropeptide Y, orexin-A, and ghrelin in differentiating unipolar and bipolar depression: a preliminary study

Abstract Background When depressive symptoms in bipolar and unipolar patients were compared, a number of studies reported that atypical vegetative features such as hypersomnia and hyperphagia were more common in bipolar patients. Moreover, neuropeptides such as orexin-A (ORX-A), ghrelin (GRL), and neuropeptide Y (NPY) are involved in the regulation of these vegetative functions. Materials and methods A total of 45 unipolar and 24 bipolar depressive patients, and 36 euthymic healthy controls were included in the study. The groups were compared in terms of peripheral blood samples of ORX-A, GRL, and NPY levels, as well as HAM-D, Epworth Sleepiness Scale, Three-Factor Eating Questionnaire-Revised, and Suicide Probability Scale scores. Results Both unipolar and bipolar patients had lower ORX-A, GRL, and NPY levels compared to the controls, whereas NPY levels of bipolar patients were lower than unipolar patients. There was a negative correlation between NPY levels and emotional eating in the bipolar group. Conclusion While lower ORX-A, GRL, and NPY levels are associated with depressive episodes regardless of the diagnosis; NPY levels also differ in bipolar and unipolar depression patients.
Feasibility of critical care ergometry: Exercise data of patients on mechanical ventilation analyzed as nine‐panel plots

Nine‐panel plots are standard displays of cardiopulmonary exercise data, used in cardiac and pulmonary medicine to investigate the nature of exercise limitation. We explored whether this approach could be used to analyze the data of critically ill patients on mechanical ventilation, capable of exercising actively. Patients followed an incremental exercise protocol using a bedside cycle ergometer. Respiratory gases were analyzed using indirect calorimetry, and blood gases were sampled from arterial catheters. Data of seven patients were combined into nine‐panel plots. Systematic analysis clarified the nature of exercise limitation in six cases. Resting metabolic rate was increased in all patients, with a median oxygen uptake ( V˙O2 ) of 5.52 (IQR 4.29–6.31) ml/kg/min. Unloaded cycling increased the V˙O2 by 19.8% to 6.61 (IQR 5.99–7.08) ml/kg/min. Adding load to the ergometer increased the V˙O2 by another 20.0% to reach V˙O2peak at a median of 7.14 (IQR 6.67–10.75) ml/kg/min, corresponding to a median extrinsic workload of 7 W. This was accompanied by increased CO2 production, respiratory minute volume, heart rate, and oxygen pulse. Three patients increased their V˙O2 to >40% of predicted V˙O2max , two patients passed the anaerobic threshold. Dead space ventilation was 44%, decreasing to 42% and accompanied by lower ventilatory equivalents during exercise. Exercise produced no net change in alveolo‐arterial PO2 difference. We concluded that diagnostic ergometry in mechanically ventilated patients was feasible. Analysis of the data as nine‐panel plots provided insight into individual limitations to exercise.
Natural killer cells: Innate immune system as a part of adaptive immunotherapy in hematological malignancies

Natural killer (NK) cells are part of a phylogenetically old defense system, which is characterized by its strong cytolytic function against physiologically stressed cells such as tumor cells and virus‐infected cells. Their use in the treatment of hematological malignancies may be more advantageous in several ways when compared with the already established T lymphocyte‐based immunotherapy. Given the different mechanisms of action, allogeneic NK cell products can be produced in a non‐personal based manner without the risk of the formidable graft‐versus‐host disease. Advanced manufacturing processes are capable of producing NK cells relatively easily in large and clinically sufficient numbers, useable without subsequent manipulations or after genetic modifications, which can solve the lack of specificity and improve clinical efficacy of NK cell products. This review summarizes the basic characteristics of NK cells and provides a quick overview of their sources. Results of clinical trials in hematological malignancies are presented, and strategies on how to improve the clinical outcome of NK cell therapy are discussed.
Biologically Inspired Modeling of Vehicle to Vehicle Communication for Intelligent Transportation Systems Applications

OF THE THESIS ii List of Illustrations vii List of Tables x CHAPTER 1: INTRODUCTION 1 CHAPTER 2: PROBLEM DEFINITION 5 CHAPTER 3: LITERATURE REVIEW 9 3.1 Vehicle Infrastructure Integration (VII) 9 3.2 Dedicated Short Range Communication (DSRC) Systems 11 3.3 In-vehicle wireless systems 12 3.4 Research on spread of information by wireless communication in vehicular networks 15 CHAPTER 4: SIMULATING VEHICLE-TO-VEHICLE COMMUNICATION IN PARAMICS 21 4.1. Extracting vehicle information 23 4.2 Processing the extracted information 24 CHAPTER 5: PRELIMINARY ANALYSIS AND RESULTS 28
Altered hippocampal expression and function of cytosolic phospholipase A2 (cPLA2) in temporal lobe epilepsy (TLE)

ABSTRACT Objectives Temporal lobe epilepsy (TLE) is the most common form of drug-resistant epilepsy. Blood–brain barrier (BBB) leakage occurs during epileptogenesis and several pieces of evidence suggest that this might contribute to the progression of epilepsy. Seizures trigger a pathway involving glutamate signalling through cytosolic phospholipase A2 (cPLA2). This pathway leads to BBB leakage and induces the expression of drug efflux transporters, leading to drug resistance. Therefore, this study aims to determine the mRNA and protein levels of cPLA2, along with its functional activity, in the hippocampus of pilocarpine model of TLE as well as in the surgically resected hippocampal samples of patients with TLE. Methods mRNA levels and protein levels of cPLA2 were evaluated by real-time PCR and western blot analysis respectively in animal model of TLE as well as surgically resected hippocampal tissue specimens of TLE. cPLA2 functional activity was measured spectrophotometrically. Results Significant up-regulation of cPLA2 mRNA was observed in the hippocampal samples obtained from TLE rats (p < 0.05) and-TLE patients (p < 0.01). Increased protein expression of cPLA2 was also demonstrated in the hippocampal samples of TLE rats (p < 0.01) as well as TLE patients (p < 0.01). Similarly, functional activity of cPLA2 was found to be up-regulated in the hippocampus of pilocarpine model of TLE rats (p < 0.01) as well as in the TLE patients (p < 0.01). Discussion These findings suggest that alterations in cPLA2 expression and activity level in the hippocampus could potentially be a part of dynamic changes associated with TLE.
Development of a classifier for gambling disorder based on functional connections between brain regions

Recently, a machine‐learning (ML) technique has been used to create generalizable classifiers for psychiatric disorders based on information of functional connections (FCs) between brain regions at resting state. These classifiers predict diagnostic labels by a weighted linear sum (WLS) of the correlation values of a small number of selected FCs. We aimed to develop a generalizable classifier for gambling disorder (GD) from the information of FCs using the ML technique and examine relationships between WLS and clinical data.
Quantity and variety of fruit and vegetable intake in midlife and cognitive impairment in late life: a prospective cohort study

Abstract There is limited evidence on fruit and vegetable intake in relation to cognitive function. This study aimed to evaluate the associations of quantity and variety in fruit and vegetable intake in midlife with cognitive impairment in late life. We used data from 16 737 participants of the Singapore Chinese Health Study, a population-based cohort study. The participants provided dietary data at recruitment at median age of 52·5 (range: 45–74) years and also participated in the third follow-up interview 20 years later at median age of 72·2 (range: 61–96) years. Quantity and variety of fruits and vegetables consumed at baseline were measured using a validated FFQ. Cognitive impairment at the third follow-up was defined using a Singapore-modified version of Mini-Mental State Examination. About 14·3 % participants had cognitive impairment. In multivariable logistic regression models, comparing extreme quartiles for intake of fruits and vegetables combined, the OR (95 % CI) associated with cognitive impairment was 0·83 (95 % CI: 0·73, 0·95; P-trend = 0·006) for quantity and 0·76 (95 % CI: 0·67, 0·87; P-trend< 0·001) for variety scores. Independently, those with increased variety of fruit intake or higher quantity of vegetable intake also had significantly 22 % and 15 % reduced odds of cognitive impairment, respectively. Finally, compared with those with low intake for both quantity and variety, those with both high quantity and variety for fruits and vegetables had 23 % reduction in odds of cognitive impairment. In conclusion, increase in quantity and variety of fruits and vegetables in midlife may reduce the risk of cognitive impairment in late life.
CsrA-Controlled Proteins Reveal New Dimensions of Acinetobacter baumannii Desiccation Tolerance

Acinetobacter baumannii is found in terrestrial environments but can cause nosocomial infections in very sick patients. A factor that contributes to the prevalence of A. baumannii in hospital settings is that it is intrinsically resistant to dry conditions. ABSTRACT Hospital environments are excellent reservoirs for the opportunistic pathogen Acinetobacter baumannii in part because it is exceptionally tolerant to desiccation. We found that relative to other A. baumannii strains, the virulent strain AB5075 was strikingly desiccation resistant at 2% relative humidity (RH), suggesting that it is a good model for studies of the functional basis of this trait. Consistent with results from other A. baumannii strains at 40% RH, we found the global posttranscriptional regulator CsrA to be critically important for desiccation tolerance of AB5075 at 2% RH. Proteomics experiments identified proteins that were differentially present in wild-type and csrA mutant cells. Subsequent analysis of mutants in genes encoding some of these proteins revealed six genes that were required for wild-type levels of desiccation tolerance. These include genes for catalase, a universal stress protein, a hypothetical protein, and a biofilm-associated protein. Two genes of unknown function had very strong desiccation phenotypes, with one of the two genes predicting an intrinsically disordered protein (IDP) that binds to DNA. Intrinsically disordered proteins are widespread in eukaryotes but less so in prokaryotes. Our results suggest there are new mechanisms underlying desiccation tolerance in bacteria and identify several key functions involved. IMPORTANCE Acinetobacter baumannii is found in terrestrial environments but can cause nosocomial infections in very sick patients. A factor that contributes to the prevalence of A. baumannii in hospital settings is that it is intrinsically resistant to dry conditions. Here, we established the virulent strain A. baumannii AB5075 as a model for studies of desiccation tolerance at very low relative humidity. Our results show that this trait depends on two proteins of unknown function, one of which is predicted to be an intrinsically disordered protein. This category of protein is critical for the small animals named tardigrades to survive desiccation. Our results suggest that A. baumannii may have novel strategies to survive desiccation that have not previously been seen in bacteria.
Efficacy and safety in the use of stent-retrievers for treatment of cerebral vasospasms after subarachnoid hemorrhage

Cerebral vasospasms are one of the most important causes of morbidity and mortality after subarachnoid hemorrhage (SAH). Spasms usually occur between day 3–14 after bleeding, with a peak around the 7th day and with an incidence of about 30%. While the term “vasospasm” describes the morphological state of pathological vasoconstriction in angiography, clinically significant vasospasm and cerebral ischemia are referred to as “delayed cerebral ischemia” (DCI). The pathophysiology of this disease is complex and multifactorial, with the contact of blood breakdown products with the abluminal vessel playing a crucial role. The diagnosis can be confirmed by transcranial Doppler sonography (TCD), crosssectional imaging with angiography, perfusion imaging and by digital subtraction angiography (DSA), which remains the gold standard. Treatment of vasospasm includes conservative and interventional approaches. However, a recent study provided Class III evidence that a frequent and early endovascular therapy to treat vasospasm reduces the risk of DCI and improves functional outcome. In interventional therapy, intra-arterial nimodipine administration is simple and safe, but not always effective and durable to clinical experience. Additionally, there is a lack of data in the literature regarding this therapy. Percutaneous balloon-PTA for severe proximal vasospasms is angiographically effective and longer-lasting in most cases but carries considerable risks such as vascular rupture and interrupted blood flow during the PTA. Therapy practices vary considerably, and optimal management remains controversial. Self-expanding stentretrievers are increasingly being used in interventional stroke therapy and seem to be a promising alternative to balloon-PTA in interventional spasmolysis due to their low periprocedural complication rates. However, only a few reports are available so far and it is not yet known how effective and safe this novel approach is, especially regarding the radial force of the retriever and the duration of the effect.8–10 We hereby report our single center experiences with interventional treatment of vasospasms using passive and active expandable stent-retrievers.
A Mixed Methods Study of Sexual Communication in Various Couple Typologies Part I: Cluster Analysis

Abstract This study presents the first part of a mixed-methods exploration of couple sexual communication. Typologies of heterosexual couples in committed, sexual relationships were created for further exploration of group differences in sexual communication. A cluster analysis categorized couples into four different couple typologies: mutually satisfied, satisfied men/dissatisfied women, satisfied women/dissatisfied men, and mutually dissatisfied. Using a MANOVA approach, the couple typologies were used to identify differences in how the couple types communicated about sex. The results revealed significant differences among the differing couple types, which will be explored qualitatively in part two of this analysis.
Age and processing effects on perceptual and conceptual priming

Explicit (declarative) memory declines with age, but age effects on implicit (nondeclarative) memory are debated. Some studies have reported null changes in implicit memory (e.g., priming in word-fragment completion, perceptual identification, category exemplar generation) with age, while others have uncovered declines. One factor that may account for these discrepancies is processing. Evidence suggests that conceptual and perceptual processes are not equally affected by ageing, yet processing requirements have varied greatly between studies. Processing may moderate age effects on priming, but no study has systematically examined this issue. This registered report presents an experiment to manipulate processing (conceptual / perceptual) during incidental encoding of words, prior to measures of perceptual (perceptual identification) and conceptual (category verification) priming. The perceptual and conceptual priming tasks were matched on all characteristics except processing, making them highly comparable. The four orthogonal conditions (perceptual encoding, perceptual test [PP]; conceptual encoding, perceptual test [CP]; perceptual encoding, conceptual test [PC]; conceptual encoding, conceptual test [CC]) were designed to clarify situations in which age effects on implicit memory emerge, which holds important practical and theoretical implications. Significant effects of Age, Test, and an Age × Processing interaction emerged. Priming was greater in young than older adults and on the perceptual than the conceptual test, but in contrast to the predictions, the age difference was only significant when prior encoding was perceptual (i.e., in the PP and CP conditions).
Mouse Adenovirus Type 1 E4orf6 Induces PKR Degradation

Viruses have evolved to counteract the immune system to successfully replicate in the host. Downregulation of several antiviral proteins is important for productive viral infection. ABSTRACT Protein kinase R (PKR) is a cellular kinase involved in the antiviral response. The inactivation or inhibition of this protein is a conserved activity in DNA and RNA virus infections. In contrast to human adenovirus type 5, mouse adenovirus type 1 (MAV-1) inhibits PKR activity through proteasome-dependent degradation. However, the molecular mechanism by which this process takes place is not fully understood. We investigated whether ubiquitination, MAV-1 early region 1B 55k (E1B 55k), and early region 4 orf6 (E4orf6) play a role in PKR degradation in MAV-1 infection, because the enzyme 3 (E3) ubiquitin ligase activity with these viral proteins is conserved among the Adenoviridae family. We provide evidence that E4orf6 is sufficient to induce mouse PKR degradation and that proteasome pathway inhibition blocks PKR degradation. Inhibition of neddylation of cullin, a component of E3 ubiquitin ligase complex, blocked efficient PKR degradation in MAV-1-infected cells. Finally, we demonstrated that MAV-1 degradation of PKR is specific for mouse PKR. These results indicate that counteracting PKR is mechanistically different in two species of adenoviruses. IMPORTANCE Viruses have evolved to counteract the immune system to successfully replicate in the host. Downregulation of several antiviral proteins is important for productive viral infection. Protein kinase R (PKR) is an antiviral protein that belongs to the first line of defense of the host. Because PKR senses dsRNA and blocks the cellular translation process during viral infections, it is not surprising that many viruses counteract this antiviral activity. We previously reported PKR degradation during mouse adenovirus type 1 (MAV-1) infection; however, the molecular mechanism of this activity was not fully known. This work provides evidence about the MAV-1 protein that induces PKR degradation and expands knowledge about involvement of the proteasome pathway.
Accurately Explaining Exploratory Decisions in a Non-Stationary Bandit Problem: A Recovery Study of the Kalman Filter Model

Daw, O’Doherty, Dayan, Seymour, and Dolan (2006) claim that a model consisting of the Kalman filter and softmax rule can be used to explain human decisions in a non-stationary four-armed bandit task. This paper aims to evaluate whether the model’s parameters can be recovered accurately, while keeping the original conditions as much as possible intact. It is shown that three parameters could not be recovered, which indicates serious identification problems. Our conclusion is that the model must be used with caution and suggestions are included to improve recovery.
Loss of RUBCN/rubicon in adipocytes mediates the upregulation of autophagy to promote the fasting response

ABSTRACT Upon fasting, adipocytes release their lipids that accumulate in the liver, thus promoting hepatic steatosis and ketone body production. However, the mechanisms underlying this process are not fully understood. In this study, we found that fasting caused a substantial decrease in the adipose levels of RUBCN/rubicon, a negative regulator of macroautophagy/autophagy, along with an increase in autophagy. Adipose-specific rubcn-knockout mice exhibited systemic fat loss that was not accelerated by fasting. Genetic inhibition of autophagy in adipocytes in fasted mice led to a reduction in fat loss, hepatic steatosis, and ketonemia. In terms of mechanism, autophagy decreased the levels of its substrates NCOA1/SRC-1 and NCOA2/TIF2, which are also coactivators of PPARG/PPARγ, leading to a fasting-induced reduction in the mRNA levels of adipogenic genes in adipocytes. Furthermore, RUBCN in adipocytes was degraded through the autophagy pathway, suggesting that autophagic degradation of RUBCN serves as a feedforward system for autophagy induction during fasting. Collectively, we propose that loss of adipose RUBCN promotes a metabolic response to fasting via increasing autophagic activity.
Judging accidental harm: Reasoning style modulates the weight of intention and harm severity

When judging a perpetrator who harmed someone accidentally, humans rely on distinct information pertaining to the perpetrator and victim. The present study investigates how reasoning style modulates the contribution of the victim’s harm and the perpetrator’s intention to third-party judgement of accidental harm. In two pre-registered online experiments, we simultaneously manipulated harm severity and the perpetrator’s intention. Participants completed reasoning measures as well as a moral judgement task consisting of short narratives which depicted the interaction between a perpetrator and a victim. In experiment 1, we manipulated the perpetrator’s intent to harm (accidental versus intentional harm) and the victim’s harm (mild versus severe harm). In experiment 2, we aimed to manipulate intent in accidental harm scenarios exclusively, using positive or neutral intents and manipulating harm severity (mild versus severe harm). As expected, intent and harm severity moderated participants’ moral judgement of acceptability, punishment, and blame. Most importantly, in both experiments, the perpetrator’s intent not only interacted with the outcome severity but also polarised moral judgements in participants with a more deliberative reasoning style. While moral judgements of more intuitive reasoners were less sensitive to intent, more deliberative reasoners were more forgiving of accidental harm, especially following mild harm. These findings extend previous studies by showing that reasoning style interacts with intent and harm severity to shape moral judgement of accidents.
Aquatic strength training improves postural stability and walking function in stroke patients

ABSTRACT Objective This study aims to explore the effects of aquatic strength training on the postural balance and walking function of stroke patients. Methods A total of 56 chronic stroke patients more than 6 months post-stroke with walking and balance impairments were included in this study. After baseline evaluations, patients were randomly assigned to either the experiment group (n = 29) or control group (n = 27). Patients in the control group underwent conventional hydrotherapy, whereas patients in the experiment group received conventional hydrotherapy combined with aquatic lower extremity strength training. After six weeks of rehabilitation, all patients were evaluated by a blinded assessor, and the functional assessments including: Berg Balance Scale (BBS); Timed Up and Go Test (TUG and mTUG); 2 Minute Walk Test (2MWMT); and Gait analysis. Results There were no significant differences (P > .05) between the two groups in all evaluation indexes before rehabilitation. Six weeks after treatments, the results from both groups showed significant improvements compared with those in the baseline evaluations (P < .05). Notably, compared with the control group, BBS, TUG and mTUG, 2MWMT, and Gait analysis including stride length in the non-hemiplegic side, stride length, as well as walking speed and stride frequency in the hemiplegic side in experiment group were markedly improved (P < .05) Conclusion Aquatic strength training can improve postural balance and lower extremity motor functions in chronic stroke patients.