Miracle-dz/UniFAR-FTUs huggingface dataset:
Avaliação do uso de “sementes” de ouro e ultrassom no tratamento do câncer por hipertermia

Cancer is currently a global epidemic and despite this, the main therapeutics modalities are limited to chemotherapy, radiotherapy and surgery. Hyperthermia is a proposal for treatment of cancer where tumor cells are affected by the increase of the local temperature according to time of exposure to the heat source and temperature range used. The aim of this study was evaluate the effects of hyperthermia obtained through a technique based on the use of ultrasound to irradiate gold seeds inserted into solid tumors. The present study evaluated the efficacy of this technique in computer models, in vitro and preclinical. To define the geometric shape and the heat diffusion of gold seed, we used a computational code based on the heat diffusion equation. From the theoretically defined parameters, a surgical technique was designed to preclinical confirmation and joint therapies using doxorubicin. Results from computer simulations show that seeds with 0,8x10mm and 1x10mm irradiated for 600s on 1,5MHz frequency produced heat generation rate of 6 x 10 W/m. In single-dose model treatment, animals were irradiated with 1 MHz for 30 min and temperature was evaluated in the central and peripheral regions of the tumor. In this treatment, increase in temperature was able to produce coagulative necrosis of 81.9 ± 7.2% of the total area of the tumor. In a three dose treatment, animals were irradiated for 15 minutes every five days. The results showed tumor inhibition rate was equal to 84.7%. The combination of pre-treatment with gold seeds irradiated with ultrasound and doxorubicin decreased the IC50 of the drug in about 50% in cells cultures and showed 87% tumor inhibition against Ehrlich carcinoma. In conclusion, the hyperthermia produced by gold seeds exposed to ultrasound is effective method for the destruction of solid tumors and can be used as an efficient adjuvant in cancer chemotherapy.
Ethanol production bySaccharomyces cerevisiae promoted by ?-alumina

The detailed examination of γ-alumina effects on the fermentation activity of the Saccharomyces cerevisiae cells as well as on the most important fermentation parameters under various experimental conditions is the purpose of the present work. The fermentation of glucose was used as a model reaction. Activity measurements were carried out at 30 o C using a batch reactor working under atmospheric pressure
Health psychology interventions to improve adherence to maintenance therapies in asthma

This is a protocol for a Cochrane Review (Intervention). The objectives are as follows: Main objectives To determine the effectiveness of theory‐based and non‐theory based health psychology interventions for improving adherence to maintenance therapy in adults with asthma Secondary objectives To compare the effectiveness of adherence interventions which are based on theory, as defined by the Theory Coding Scheme (TCS), to interventions which are not theory‐based To identify and describe, using the TSC and Theoretical Domain Framework (TDF), the different health psychology theories which have been used in interventions to improve adherence to maintenance therapy in adults with asthma To evaluate the extent to which health psychology theory has been applied to the development of adherence interventions in asthma
The role of Elongator complex in Saccharomyces cerevisiae and Caenorhabditis elegans

The elongator (ELP) complex consisting of Elp1-6p has been indicated to play roles in multiple cellular processes. In yeast, the ELP complex has been shown to genetically interact with Uba4p/Urm1p and Kti11-13p for a function in tRNA modification. Through a Caenorhabditis elegans genetic suppressor screen and positional cloning, we discovered that loss-of-function mutations of moc-3 and dph-3, orthologs of the yeast UBA4 and KTI11, respectively, effectively suppress the Multivulva (Muv) phenotype of the lin-1(e1275, R175Opal) mutation. These mutations do not suppress the Muv phenotype caused by other lin-1 alleles or by gain-of-function alleles of ras or raf that act upstream of lin-1. The suppression can also be reverted by RNA interference of lin-1. Furthermore, we showed that dph-3(lf) also suppressed the defect of lin-1(e1275) in promoting the expression of a downstream target (egl-17). These results indicate that suppression by the moc-3 and dph-3 mutations is due to the elevated activity of lin-1(e1275) itself rather than the altered activity of a factor downstream of lin-1. We further showed that loss-of-function mutations of urm-1 and elpc-1-4, the worm counterparts of URM1 and ELP complex components in yeast, also suppressed lin-1(e1275). We also confirmed that moc-3(lf) and dph-3(lf) have defects in tRNA modifications as do the mutants of their yeast orthologs. These results, together with the observation of a likely readthrough product from a lin-1(e1275)∷gfp fusion transgene indicate that the aberrant tRNA modification led to failed recognition of a premature stop codon in lin-1(e1275). Our genetic data suggest that the functional interaction of moc-3/urm-1 and dph-3 with the ELP complex is an evolutionarily conserved mechanism involved in tRNA functions that are important for accurate translation.
Meta-analysis of Change in Weight and Heart Rate for Phentermine in Obesity

Obesity is defined as a body mass index (BMI) of ≥ 30 kg/m [1]. In Korea, obesity is defined as BMI of 25 kg/m based on the WHO AsiaPacific perspective for Asians [2,3]. The prevalence of obesity defined by BMI >25 kg/m was increased from 36.2% in 2011 to 38.9% in 2016. The prevalence rate of obesity has also been > 60% in America from 2012 [4]. Obesity needs to be considered as chronic disease [5]. It was relevant to cardiovascular diseases [6], and a report showed that approximately 40% of the mortality rate in the U.S. was due to obesity or obesity-related diseases in 2000 [7]. To reduce body weight, a weight control program that includes a dietary therapy and exercise therapy needs to be tried [8], and an appetite suppressant has been generally prescribed for obesity care [9]. Phentermine, one of the popular appetite suppressants, was approved Meta-analysis of Change in Weight and Heart Rate for Phentermine in Obesity Yeonju Woo, Hyomi Jeong Researcher, Institute of Korean Medical Science, Pusan National University, Yangsan; Manager, LSK Global Pharma Services, Seoul, Korea
Established and Emerging Cardiovascular Magnetic Resonance Imaging Techniques in the Evaluation of Subclinical Cardiovascular Disease

Introduction: Cardiovascular disease (CVD) remains the number one cause of mortality in the world for both men and women, thus improving its diagnosis and treatment is a priority. Rheumatoid Arthritis (RA) is a common auto-immune disease associated with high rates of CVD. Cardiovascular magnetic resonance (CMR) offers a multi-parametric, quantitative approach to the assessment of the heart and cardiovascular system with a host of techniques allowing assessment of anatomy, ventricular function, myocardial composition, myocardial perfusion, vascular performance and myocardial metabolism during a single scan. Its quantitative nature and lack of ionising radiation lend themselves ideally to the longitudinal study of subclinical CVD. Aims: To assess 1) whether blood longitudinal relaxation (T1) can be used to estimate blood haematocrit value to allow calculation of extracellular myocardial volume fraction (ECV), 2) whether CMR feature tracking (CMR-FT) is a feasible means of assessing aortic stiffness, 3) whether global longitudinal strain (GLS) is reduced in patients with prior MI with preserved left ventricular ejection fraction versus healthy controls, 4) whether aortic stiffness is present at all time points in the disease course of RA and 5) whether subclinical CV abnormalities in newly diagnosed RA improve with treatment and if the anti-tumour necrosis α Inhibitor Etanercept offers additional benefit over standard treatment. Methods: Patients were recruited between and February 2011 and February 2017. All patients underwent a comprehensive, multi-parametric CMR study including cine and late Gadolinium enhancement imaging at either 1.5 or 3.0T. Results: 1) estimation of blood haematocrit from blood T1 value provides accurate estimation of ‘synthetic’ ECV, 2) CMR-FT assessment of aortic stiffness is feasible and provides reproducible values for descending and ascending aortic strain values, 3) GLS is reduced in prior MI patients versus healthy controls (-17.3 ± 3.7% versus -19.3 ± 1.9% respectively, p=0.012). A GLS cut-off value of 18% correctly identifies prior MI with a sensitivity of 60% and specificity of 72.5%, 4) Aortic stiffness is evident in at risk RA individuals, newly diagnosed RA as well as established RA and 5) Aortic distensibility and left ventricular mass improve significantly in newly diagnosed RA patients following treatment. Etanercept appears to offer additional benefit over standard treatment evidenced by numerical improvements in aortic distensibility.
Propensity Score-Matching Methods for Observational Studies: An Application to Stat 216 Data

Many fields of science are faced with the inability to perform randomized experiments, but wish to have the ability to estimate a treatment effect and make causal inference. Propensity score matching is a method that can be used in observational studies to obtain unbiased estimates of the treatment effect. In this paper we consider the theory behind utilizing propensity score matching to obtain these such estimates, as well as explain how to implement propensity score matching in R using the Matching package for data from Montana State University’s Introductory Statistics curriculum.
The Study of the Relationship between Alexithymia and Type D Personality with Perceived Stress as Mediator on the Blood Pressure of Heart Patients

The research aimed to study the relationship between alexithymia and type D personality with stress as the mediator variable on blood pressure of heart patients. The research method is descriptive and correlational. The research population consisted of all heart patients who referred to Imam Reza Hospital of Mashahd in 2014. Available sampling method was used and 200 patients have been selected from 623 heart patients. The participants filled the demographic, perceived stress, type D personality and Farsi version of alexithymia questionnaires, and data were analyzed using path analysis and correction coefficient. Results showed that type D personality and alexithymia with perceived stress as mediator can increase blood pressure and other heart diseases. Path analysis pattern had been approved and accordingly, the path of type D personality to blood pressure with standard coefficient of 0.24 and the path of stress to blood pressure with standard coefficient of 0.22 were significant. Moreover, the path of alexithymia to blood pressure with standard coefficient of 0.11 and the path of alexithymia to stress with standard coefficient of 0.02 weren’t significant. It must be noted that in this causal model, Type D personality direction to stress, alexithymia to blood pressure, and alexithymia to stress don’t have casual effect and the relationship is just correlational. It can be concluded that psychological variables such as type D personality and alexithymia and perceived stress can increase physical chronic disorders such as blood pressure and other heart diseases.
The Role of Kisspeptin and KNDy Cells in the Reproductive Neuroendocrine System

s Merkley CM, Frazao R. , Coolen, LM, Clegg DJ, Elias CF, Lehman MN. (November 6-9, 2012). Estrogen-induced plasticity in glutamatergic inputs to Kiss1 neurons in the mouse. Oral presentation at the 2 nd World Conference on Kisspeptin Signaling in the Brain, Tokyo, Japan
Role of imaging in Alzheimer's disease

Alzheimer's disease (AD) is the most common cause of dementia and its prevalence is expected to increase in the coming years. Therefore, accurate diagnosis is crucial for patients. The emerging imaging techniques have provided important information for the clinical practice and management of the disease. Computed tomography, magnetic resonance imaging, positron emission tomography, single-photon emission computed tomography and in vivo amyloid imaging are currently available for clinical use.
Associations between nailfold capillaroscopy findings and interstitial lung disease in patients with mixed connective tissue disease

Introduction Mixed connective tissue disease (MCTD) is a chronic immune-mediated disorder defined by the combined presence of serum anti-RNP antibodies and distinct clinical features including progressive lung fibrosis. The aim of this study was to evaluate potential associations between lung fibrosis in MCTD and specific clinical and laboratory findings including results of nailfold capillaroscopy (NFC) examination. Material and methods Patients with MCTD who were admitted to the Departments of Allergy and Immunology or Dermatology at the University Hospital of Krakow (Poland) in 2015–2018 were identified based on comprehensive individual record review. Diagnosis of MCTD required fulfilment of at least one of the four widely accepted sets of diagnostic criteria. Clinical and laboratory data were collected, and statistical analysis was performed to identify potential predictors of interstitial lung disease (ILD). Results Thirty patients (90% females) aged 22–81 years met the study inclusion criteria. The mean duration of symptoms associated with MCTD was 7.3 months. Photosensitivity and Raynaud’s phenomenon were the most common clinical manifestations (90% and 70%, respectively). Hand oedema, sclerodactyly and the presence of giant capillaries in NFC correlated significantly with the risk of lung involvement. In multivariate analysis, the presence of enlarged loops in NFC (giant capillaries) was identified as an independent factor for ILD (R 2 = 0.82, p < 0.0000001). Conclusions The NFC examination should be considered in all patients with a diagnosis of MCTD. The presence of giant capillaries may be a promising marker for interstitial lung disease in these patients, especially among those with a short duration of disease (i.e. < 1 year).
Tracking turtles to their death — reply to Hays et al.

We welcome the opportunity to respond to the comments of Hays et al. (2004, this volume) on our comprehensive analysis of satellite telemetry and its use to estimate post-hooking mortality in marine turtles (Chaloupka et al 2004). Our paper was the first to provide robust estimates of post-hooking mortality for any sea turtle sample. We raised a number of concerns about the applicability of the platform terminal transmitter satellite telemetry used by Hays et al. (2003) to derive estimates of fishery-specific mortality. None of these concerns have been addressed in the Hays et al. (2004) Comment. However, Hays et al. (2004) believe that we possibly misunderstood 2 aspects of their study. Specifically, they suggest –– contrary to our assertion otherwise –– that they did in fact know the cause of each transmitter failure. This is incorrect. They state that:
Big data, artificial intelligence, and cardiovascular precision medicine

ABSTRACT Introduction: Cardiovascular diseases (CVDs) are chronic, heterogeneous diseases which are generally classified according to clinical presentation. However, the arrival of big data and analytical methods presents an opportunity to better understand these disease entities. Areas covered: This review article highlights: (1) the potential of a big data approaches with emerging technology to explore the heterogeneity of CVDs; (2) current challenges of a big data approach; and (3) the future of precision cardiovascular medicine. Expert commentary: Overall, most of the current data utilizing big data techniques remain largely descriptive and retrospective. Precision medicine, or N-of-1, approaches have not yet allowed for consistent interpretation since there is no ‘standard’ of how to best apply treatment approaches in a field where evidence-based medicine is based largely on randomized controlled trials. The risk score and biomarker-based approaches have been utilized with some ‘validation’ studies, but more in-depth biomarkers (i.e. pharmacogenomic biomarkers) have failed to demonstrate incremental benefits. Exploring novel CVD phenotypes by integrating existing medical variables, multi-omics, lifestyle, and environmental data using artificial intelligence is vitally important and may allow us to digitize future clinical trials, potentially leading to novel therapies.
Comparative evaluation of the anti-plaque efficacy of green tea extract mouthrinse and white tea extract mouthrinse with chlorhexidine gluconate mouthrinse-A clinical and microbiological study

Introduction: Numerous studies have been conducted based on the natural mouthwashes, with fewer side effects, as an alternative to chemical ones. The aim of this study is to compare clinically and microbiologically the anti plaque efficacy of mouthwash containing extract of green tea, white tea extract with chlorhexidinegluconate mouthwash. Materials and Methods: This clinical study was performed in the department of Periodontology and Oral Implantology, I.T. S Dental College, Muradnagar, Ghaziabad, India. All the patients were educated about the study and then a written informed consent was acquired before enrolment in the study. The study was conducted for a period of 10 days. A total of 30 Dental students were selected as patients of age group of 19-23 years(from 1st to 2nd year) according to assigned inclusion criteria, oral prophylaxis was performed & the subjects were randomly allocated equally to one of the three treatment groups (10 each) Group A – Green Tea Extract , Group B – White Tea Extract , Group C- Chlorhexidinegulconate. The subjects were assessed both preoperatively and postoperatively clinically by recording the plaque and gingival indices and microbiologically by counting the number of colonies forming units/mL (CFU/mL). Results: Green Tea showed statistically significant difference in clinical parameters and in CFUs/ml compared to White Tea. Conclusion: The results of the current study indicated that White Tea and Green Tea mouth rinse were found to be a potent anti-plaque agent although less efficacious than chlorhexidine mouth rinse. Green tea catechin mouthwash was comparably efficacious to chlorhexidinegluconate as an antiplaque agent when used for 10 days. Keywords: Green tea, White tea, Chlorhexidinegluconate mouth rinse, Antiplaque.
Evolution of the Phosphatase Gene Family Across Nematode Worms and Flies

Phosphatase genes have been shown to be involved in male meiosis in the nematode worm, Caenorhabditis elegans, and are expressed in the testis in the fruit fly, Drosophila melanogaster. However, the evolution of this multi-gene family among nematodes and flies had not previously been investigated. We conducted a phylogenetic analysis of all genes in the phosphatase gene family across nematodes and flies using sequences from a 6-way alignment of nematode worms and a 15- way alignment of insects, including 12 Drosophila species. We found that: 1) multiple alignments contain spurious alignments that should be filtered for quality control; 2) several gene sequences with incomplete open reading frames are highly conserved, so may actually be functional genes; and, 3) the phosphatase gene family appears to have expanded independently in the common ancestor of nematodes, and again in the common ancestor of flies (but not all insects).
The Role of BCA2 in the Endocytic Trafficking of EGFR and Significance as a Prognostic Biomarker in Cancer

Breast Cancer Associated gene 2 (BCA2) is an E3 ubiquitin ligase that is over-expressed in >50% of primary breast cancers, and has been shown to increase in vitro cell proliferation and invasion. The protein has been linked to alterations in EGFR degradation; however there is some dispute as to its role and influence on the biology of this receptor. Our work aimed to ascertain the role of BCA2 in EGFR endocytosis and down-regulation and to examine its links with breast cancer outcome. Data generated with the online expression analysis tool KM-Plotter showed that high BCA2 levels are associated with poor prognosis in ovarian, gastric and breast cancer, particularly HER2 over-expressing breast cancers. Experimentally, we demonstrate that over-expression of BCA2 induced a reduction in total EGFR levels. BCA2 over-expressing cells stimulated with EGF exhibited reduced lysosomal degradation of both this ligand and its receptor. Signalling downstream of EGFR in BCA2 over-expressing cells was characterized by a lower magnitude but increased duration. Our findings support a role for BCA2 in receptor endocytosis. Consistent with this we show that BCA2 over-expression reduces the level of vesicle-associated Rab7, a regulator of late endocytosis and documented interaction partner of BCA2. Levels of transferrin receptor and the uptake of transferrin were unaltered by over-expression of BCA2 indicating that trafficking changes may be limited to late endocytic sorting events. This report offers a thorough exploration of BCA2 biology and suggests a context-dependent role for the protein in the endocytic regulation of EGFR and as a prognostic biomarker in cancer.