Miracle-dz/UniFAR-FTUs huggingface dataset:
Elucidating the Quenching Mechanism in Carbon Dot-Metal Interactions–Designing Sensitive and Selective Optical Probes

Overexposure to metals has significant adverse effects on human and animal health coupled with nefarious consequences to the environment. Sensitive tools to measure low contaminant levels exist, but often come at a high cost and require tedious procedures. Thus, there exists a need for the development of affordable metal sensors that can offer high sensitivity and selectivity while being accessible on a global scale. Here, carbon dots, prepared in a one-pot synthesis using glutathione and formamide, have been developed as dual fluorescent metal sensing probes. Following extensive characterization of their physico-chemical properties, it is demonstrated that dual fluorescence can be exploited to build a robust ratiometric sensor with low-ppb detection sensitivity in water. This investigation shows that these optical probes are selective for Pb2+ and Hg2+ ions. Using steady-state and dynamic optical characterization techniques, coupled with hard and soft acid-base theory, the underlying reason for this selective behavior was identified. These findings shed light on the nature of metal-carbon dot interactions, which can be used to tailor their properties to target specific metal ions. Finally, these findings can be applicable to other fluorescent nanoparticle systems that are targeted for development as metal sensors.
Molecular Epidemiology and Mechanisms of Carbapenem-Resistant Acinetobacter baumannii Isolates from ICU and Respiratory Department Patients of a Chinese University Hospital

Background The objective of our study is to estimate the differences in molecular epidemiology and resistance mechanisms in carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from the ICU and respiratory department(RD) in Fourth Affiliated Hospital of Harbin Medical University. Methods Carbapenemase genes associated with carbapenem resistance were studied by polymerase chain reaction(PCR). Genotyping was analyzed using multi-locus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE). Results Sixty non-duplicate CRAB isolates from the ICU and RD (n=30, respectively) were collected. All of CRAB strains were not resistant to colistin (0%). The CRAB strains from the ICU were significantly more resistant to tigecycline and cefoperazone/sulbactam compared with the RD (23.3% vs 0%, P=0.03; 53.3% % vs 23.3%, P=0.01, respectively). PCR detection of genes associated with CRAB revealed that the ratio in both the ICU and the RD of blaVIM-2, blaIMP-4, blaNDM-1, blaOXA-23, ampC, and mutation of CarO were present in 23.3% vs 0% (P=0.01), 40% vs 10% (P=0.02), 20% vs 0% (P=0.02), 80% vs 56.7%, 16.7% vs 13.3% and 86.7% vs 60% (P=0.04), respectively. Seven genotypes were detected by the PFGE in the RD and the ICU, respectively. Genotype I was significantly more frequent in the ICU compared with the RD (63.3% vs 36.6%, P=0.03). MLST showed that there were 10 ST genotypes in the RD and four in the ICU, but ST92 in both groups was 33.3% vs 63.3% (P=0.03), respectively. Conclusion There are differences in molecular epidemiology and resistance mechanisms in the CRAB isolates between the ICU and RD.
Clinical Utility of Eribulin Mesylate in the Treatment of Breast Cancer: A Chinese Perspective

Abstract Eribulin mesylate, a synthetic derivative of the anti-mitotic agent halichondrin B, has a unique tubulin-based mechanism of action that is distinct from other anti-microtubule agents including taxanes and vinca alkaloids. Consistent with this unique activity, eribulin has shown clinical efficacy in patients with metastatic breast cancer (MBC) that progressed following prior taxane and anthracycline therapy. The evidence presented in this review indicates that eribulin represents a treatment option for patients with HER2-negative metastatic breast cancer. Improved survival outcomes and better tolerability compared with vinorelbine supported the first approval of eribulin in China in 2019; eribulin was approved for women with locally advanced/metastatic HER2-negative breast cancer after treatment failure with at least two chemotherapy regimens, including an anthracycline and a taxane. Eribulin has also shown promising efficacy in patients with HER2-positive advanced breast cancer when used in combination with trastuzumab or pertuzumab, and subgroup analyses from the Phase III clinical trials support the continued evaluation of eribulin in patients with triple-negative disease. The unique non-mitotic effects of eribulin, including vascular remodeling, coupled with its clinical efficacy and safety profile, may permit the broader use of this agent in patients with MBC.
Summated Hazard Score as a Powerful Predictor of Fatigue in Relation to Pacing Strategy

During competitive events, the pacing strategy depends upon how an athlete feels at a specific moment and the distance remaining. It may be expressed as the Hazard Score (HS) with momentary HS being shown to provide a measure of the likelihood of changing power output (PO) within an event and summated HS as a marker of how difficult an event is likely to be perceived to be. This study aimed to manipulate time trial (TT) starting strategies to establish whether the summated HS, as opposed to momentary HS, will improve understanding of performance during a simulated cycling competition. Seven subjects (peak PO: 286 ± 49.7 W) performed two practice 10-km cycling TTs followed by three 10-km TTs with imposed PO (±5% of mean PO achieved during second practice TT and a self-paced TT). PO, rating of perceived exertion (RPE), lactate, heart rate (HR), HS, summated HS, session RPE (sRPE) were collected. Finishing time and mean PO for self-paced (time: 17.51 ± 1.41 min; PO: 234 ± 62.6 W), fast-start (time: 17.72 ± 1.87 min; PO: 230 ± 62.0 W), and slow-start (time: 17.77 ± 1.74 min; PO: 230 ± 62.7) TT were not different. There was a significant interaction between each secondary outcome variable (PO, RPE, lactate, HR, HS, and summated HS) for starting strategy and distance. The evolution of HS reflected the imposed starting strategy, with a reduction in PO following a fast-start, an increased PO following a slow-start with similar HS during the last part of all TTs. The summated HS was strongly correlated with the sRPE of the TTs (r = 0.88). The summated HS was higher with a fast start, indicating greater effort, with limited time advantage. Thus, the HS appears to regulate both PO within a TT, but also the overall impression of the difficulty of a TT.
Heterogeneous Response to Immunotherapy in a Patient with Tonsillar Squamous Cell Carcinoma Assessed by 18F-FDG PET/CT

Tonsillar carcinoma is the second most common malignancy of the head and neck region, with Squamous Cell Carcinoma (TSCC) as the most common histological type (>90%). For the advanced stage of TSCC, radiotherapy with or without platinum-based chemotherapy is the only therapeutic option. Immuno-checkpoint inhibitors (ICIs), in particular Nivolumab, considerably improves clinical management of these patients, but the response can be unpredictable. Difficulties can be encountered in evaluating response to immunotherapy, especially with morphological imaging, which can show an atypical response, such as pseudo-progression, leading to a premature discontinuation. Conversely, metabolic imaging can guide a more properly therapeutic decision. We present a case of a 71-year-old man affected by TSCC, treated with chemotherapy, radiotherapy, and Nivolumab as the last line of treatment. Pre- and post-immunotherapy 18F-FDG PET/CT showed an impressive response, avoiding early drug discontinuation and ensuring better management of this patient.
The complete chloroplast genome of a distinctive meadow-rue, Thalictrum foeniculaceum (Ranunculaceae)

Abstract Thalictrum foeniculaceum is a morphologically distinctive species in the genus with very reduced filiform compound leaves and big pinkish flowers occurring in dry slopes of northern China. Herein, we report the first complete chloroplast genome sequence of T. foeniculaceum. The chloroplast genome sequence was 155,923 bp in length, with large and small single-copy regions (LSC with 85,323 bp and SSC with 17,628 bp in length) separated by two inverted repeat regions (IRs with 26,486 bp). The total GC content was 38.3%. The complete plastome sequence contained 111 genes, including 77 protein-coding, 30 tRNA, and four rRNA genes. The phylogenetic analysis of Thalictrum based on the complete chloroplast genomes available online was also presented in this study.
Comparative anatomy and salt management of Sonneratia caseolaris (L.) Engl. (Lythraceae) grown in saltwater and freshwater

Sonneratia caseolaris is a pioneer species in mangrove. It can naturally grow in both saltwater and freshwater. The study was aimed at investigating and comparing the anatomical character of the S. caseolaris plants growing in different conditions and how they coped with salinity. The anatomical characteristics of roots, stems, petioles and leaf blade were investigated. The plant samples were prepared into permanent slides using a paraffin method, while the wood samples were made into permanent slides using a sliding microtome technique. Tissue clearing of leaf blade and scanning electron microscopic analysis of wood were performed. In addition, sodium chloride content in various organs and tissues was examined. It was found that cable root, stem and leaf blade showed some different anatomical characteristics between the two conditions. Periderm is a prominent tissue in saltwater roots. Tanniferous cells were observed in pneumatophores, petioles, stems and leaf blades of saltwater plants, but not found in pneumatophores and lamina of freshwater plants. Mesophyll thickness was lower in the saltwater condition. The vessel density was significantly higher in the saltwater condition than in the freshwater condition, whereas the vessel diameters in the freshwater condition were significantly higher than those in the saltwater condition. From the results, it can be concluded that root periderm plays an important role in salt exclusion, and the occurrence of tanniferous cells is associated with salt elimination.
A Study on Using the Additive Manufacturing Process for the Development of a Closed Pump Impeller for Mechanically Pumped Fluid Loop Systems

The efficiency of a centrifugal pump for mechanical pump fluid loops, apart from the design, relies on the performance of the closed impeller which is linked to the manufacturing process in terms of dimensional accuracy and the surface quality. Therefore, the activities of this paper were focused on defining the manufacturing process of a closed impeller using the additive manufacturing technology for mechanically pumped fluid loop (MPFL) systems in space applications. Different building orientations were studied to fabricate three closed impellers using selective laser melting technology and were subjected to dimensional accuracy and surface quality evaluations in order to identify the optimal building orientation. The material used for the closed impeller is Inconel 625. The results showed that both geometrical stability and roughness were improved as the building orientation increased, however, the blade thickness presented small deviations, close to imposed values. Finishing processes for inaccessible areas presented significant results in terms of roughness, nevertheless, the process can be further improved. Abrasive flow machining (AFM) post-processing operations have been considered and the results show major improvements in surface quality. Thus, important steps were made towards the development of complex structural components, consequently increasing the technological readiness level of the additive manufacturing process for space applications.
Heterogeneous catalytic epoxidation of fatty acid methyl esters on titanium-grafted silicas

Three different titanium-grafted silicates, with different morphological features were compared in the epoxidation, using tert-butylhydroperoxide as oxidant, of methyl oleate, methyl elaidate and of a mixture of methyl esters, obtained from high-oleic sunflower oil. Ti-MCM-41, a well-ordered mesoporous catalyst, showed very high conversions and excellent selectivity on all the substrates. The fatty acid methyl esters derived from renewable raw material were epoxidised selectively with very high yields in a reaction medium completely free from organic acidic compounds. The influence of the pore order of the catalysts on the catalytic performance was also examined. The interactions between the organic substrate and the ordered array of mesopores in Ti-MCM-41 were evidenced.
Fat mass prediction equations and reference ranges for Saudi Arabian Children aged 8–12 years using machine technique method

Background The number of children with obesity has increased in Saudi Arabia, which is a significant public health concern. Early diagnosis of childhood obesity and screening of the prevalence is needed using a simple in situ method. This study aims to generate statistical equations to predict body fat percentage (BF%) for Saudi children by employing machine learning technology and to establish gender and age-specific body fat reference range. Methods Data was combined from two cross-sectional studies conducted in Saudi Arabia for 1,292 boys and girls aged 8–12 years. Body fat was measured in both studies using bio-electrical impedance analysis devices. Height and weight were measured and body mass index was calculated and classified according to CDC 2,000 charts. A total of 603 girls and 374 boys were randomly selected for the learning phase, and 153 girls and 93 boys were employed in the validation set. Analyses of different machine learning methods showed that an accurate, sensitive model could be created. Two regression models were trained and fitted with the construction samples and validated. Gradient boosting algorithm was employed to achieve a better estimation and produce the equations, then the root means squared error (RMSE) equation was performed to decrease the error. Body fat reference ranges were derived for children aged 8–12 years. Results For the gradient boosting models, the predicted fat percentage values were more aligned with the true value than those in regression models. Gradient boosting achieved better performance than the regression equation as it combined multiple simple models into a single composite model to take advantage of that weak classifier. The developed predictive model archived RMSE of 3.12 for girls and 2.48 boys. BF% and Fat mass index charts were presented in which cut-offs for 5th, 75th and 95th centiles are used to define ‘under-fat’, ‘normal’, ‘overfat’ and ‘subject with obesity’. Conclusion Machine learning models could represent a significant advancement for investigators studying adiposity-related issues in children. These models and newly developed centile charts could be useful tools for the estimation and classification of BF%.
Identification of Key Candidate Genes Involved in the Progression of Idiopathic Pulmonary Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a lethal, agnogenic interstitial lung disease with limited therapeutic options. To investigate vital genes involved in the development of IPF, we integrated and compared four expression profiles (GSE110147, GSE53845, GSE24206, and GSE10667), including 87 IPF samples and 40 normal samples. By reanalyzing these datasets, we managed to identify 62 upregulated genes and 20 downregulated genes in IPF samples compared with normal samples. Differentially expressed genes (DEGs) were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to illustrate relevant pathways of IPF, biological processes, molecular function, and cell components. The DEGs were then subjected to protein–protein interaction (PPI) for network analysis, serving to find 11 key candidate genes (ANXA3, STX11, THBS2, MMP1, MMP9, MMP7, MMP10, SPP1, COL1A1, ITGB8, IGF1). The result of RT-qPCR and immunohistochemical staining verified our finding as well. In summary, we identified 11 key candidate genes related to the process of IPF, which may contribute to novel treatments of IPF.
New building blocks for molecular spintronics

In der vorliegenden Arbeit werden die drei generellen Schritte beim Aufbau neuer Materialien aus funktionellen Bausteinen an Beispielen nachvollzogen. Im Einzelnen sind dies die Verknupfung bekannter Funktionselemente und die Identifikation der Strukturelemente neuer Funktionen, die Darstellung von funktionalen Bausteinen mit Verknupfungsstellen und die Verknupfung der Bausteine und der Nachweis, dass die Funktion dadurch nicht verloren geht. Die Synthese von neuen Bausteinen steht dabei ebenso im Fokus wie die ausgedehnte Analyse erhaltener Verbindungen. Zwei neue Ligandgeruste werden beschrieben, die hochfunktionale Triphenylamineinheiten enthalten. Des Weiteren werden Verbindung auf der Basis von Triaminobenzen, Triaminoguanidin, Mesalen-artigen Liganden und acetylsubstituierten Phloroglucin untersucht und sorgfaltig bezuglich ihrer magnetischen Eigenschaften charakterisiert. Erhaltene Ergebnisse konnten anschliesend erfolgreich in andere Materialklassen ubertragen werden: Die magnetische Anomalitat, wie sie in Verbindungen basierend auf Triaminobenzen gefunden wird, konnte erfolgreich in Carbazolverbindungen integriert werden. Ein ubergreifendes Ergebnis dieser Arbeit sind die Eigenschaften, die sich beim Einbau von Sulfonatgruppen in Verbindungen ergeben: Die Losungseigenschaften der erhaltenen Verbindungen werden stark geandert, wobei die wasserloslichen Produkte in Polyvinylalkoholfolien eingebettet werden konnen, was einen viel versprechenden Ansatz fur zukunftige Materialien darstellt. Die Sulfonatsubtitution begunstigt auf Grund der Koordinationsfahigkeiten der Sulfonatgruppe die Ausbildung ausgedehnter Strukturen und bietet neue Strukturen durch die Bindung von Ammoniumgruppen mittels mehrfacher Wasserstoffbruckenbindungen an Mesalenartige Metallkomplexe.
Biosignal Sensors and Deep Learning-Based Speech Recognition: A Review

Voice is one of the essential mechanisms for communicating and expressing one’s intentions as a human being. There are several causes of voice inability, including disease, accident, vocal abuse, medical surgery, ageing, and environmental pollution, and the risk of voice loss continues to increase. Novel approaches should have been developed for speech recognition and production because that would seriously undermine the quality of life and sometimes leads to isolation from society. In this review, we survey mouth interface technologies which are mouth-mounted devices for speech recognition, production, and volitional control, and the corresponding research to develop artificial mouth technologies based on various sensors, including electromyography (EMG), electroencephalography (EEG), electropalatography (EPG), electromagnetic articulography (EMA), permanent magnet articulography (PMA), gyros, images and 3-axial magnetic sensors, especially with deep learning techniques. We especially research various deep learning technologies related to voice recognition, including visual speech recognition, silent speech interface, and analyze its flow, and systematize them into a taxonomy. Finally, we discuss methods to solve the communication problems of people with disabilities in speaking and future research with respect to deep learning components.
Mucoadhesive Gelatin Buccal Films with Propranolol Hydrochloride: Evaluation of Mechanical, Mucoadhesive, and Biopharmaceutical Properties

This study processes and characterizes propranolol hydrochloride/gelatin mucoadhesive buccal films. Two types of gelatin are used: Gelatin from porcine skin, type A (GA), and gelatin from bovine skin (GB). The influence of gelatin type on mechanical, mucoadhesive, and biopharmaceutical characteristics of buccal films is evaluated. Fourier-Transfer infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analysis show that GA with propranolol hydrochloride (PRH) in the film (GAP) formed a physical mixture, whereas GB with PRH (GBP) form a compound-complex. Results of mechanical testing (tensile test, hardness) revealed that GAP films exhibit higher elastic modulus, tensile strength, and hardness. A mucoahesion test shows that GBP has higher adhesion strength, while GAP shows higher work of adhesion. Both in vitro release study and in silico simulation indicated that processed films can provide effective drug transport through the buccal mucosa. In silico simulation shows improved bioavailability from buccal films, in comparison to the immediate-release tablets—indicating that the therapeutic drug dose can be markedly reduced.
Economic Evaluation in Neurological Physiotherapy: A Systematic Review

This systematic review was carried out to compile and assess original studies that included economic evaluations of neurological physiotherapy interventions. A thorough search of PubMED, Cochrane and Embase was developed using keywords such as health economics, neurological physiotherapy and cost analysis, and studies published during the last six-year term were selected. A total of 3124 studies were analyzed, and 43 were eligible for inclusion. Among the studies analyzed, 48.8% were interventions for stroke patients, and 13.9% were focused on Parkinson’s disease. In terms of the countries involved, 46.5% of the studies included were developed in the UK, and 13.9% were from the USA. The economic analysis most frequently used was cost-utility, implemented in 22 of the studies. A cost-effectiveness analysis was also developed in nine of those studies. The distribution of studies including an economic evaluation in this discipline showed a clear geographic dominance in terms of the pathology. A clear upward trend was noted in the economic evaluation of interventions developed in neurological physiotherapy. However, these studies should be promoted for their use in evidence-based clinical practice and decision-making.
Organoid and Spheroid Tumor Models: Techniques and Applications

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and spheroids are different 3D cell culture models that can be cultured with different techniques. These 3D cell culture units established from a patient tumor have several similarities to the original tumor tissue and possess several advantages in conducting basic and clinical cancer research. Organoids prepared from a patient tissue can be preserved in a living biobank. Testing chemo-, radio- and immuno-therapies on these organoids has the potential to predict the patient responses and these models have incredible promise for personalized medicine. This review presents different organoid models, the techniques to prepare them and recent advances in their applications. Abstract Techniques to develop three-dimensional cell culture models are rapidly expanding to bridge the gap between conventional cell culture and animal models. Organoid and spheroid cultures have distinct and overlapping purposes and differ in cellular sources and protocol for establishment. Spheroids are of lower complexity structurally but are simple and popular models for drug screening. Organoids histologically and genetically resemble the original tumor from which they were derived. Ease of generation, ability for long-term culture and cryopreservation make organoids suitable for a wide range of applications. Organoids-on-chip models combine organoid methods with powerful designing and fabrication of micro-chip technology. Organoid-chip models can emulate the dynamic microenvironment of tumor pathophysiology as well as tissue–tissue interactions. In this review, we outline different tumor spheroid and organoid models and techniques to establish them. We also discuss the recent advances and applications of tumor organoids with an emphasis on tumor modeling, drug screening, personalized medicine and immunotherapy.