Miracle-dz/UniFAR-FTUs huggingface dataset:
Assessment of Fungal Contamination in Fish Feed from the Lake Victoria Basin, Uganda

The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried out to examine fungal colonization and mycotoxin contamination in fish feed samples (n = 147) of different types collected from nine fish farms (n = 81) and seven fish feed factories (n = 66) in the Lake Victoria Basin (LVB). Fungi were isolated in potato dextrose agar, grouped into morphotypes and representative isolates from each morphotype were identified based on the internal transcribed spacer (ITS) region of ribosomal DNA sequences. Aflatoxin B1 (AFB1) and total fumonisin (combinations of B1, B2 and B3; hereinafter named fumonisin) levels in feed samples were determined by enzyme-linked immunosorbent assay (ELISA). A wide range of fungi, including toxigenic Aspergillus flavus and Fusarium verticillioides, were isolated from the fish feed samples. AFB1 was detected in 48% of the factory samples and in 63% of the farm samples, with toxin levels <40 and >400 µg/kg, respectively. Similarly, 31% of the factory samples and 29% of the farm samples had fumonisin contamination ranging between 0.1 and 4.06 mg/kg. Pellets and powder had higher mycotoxin contamination compared to other commercially available fish feed types. This study shows AFB1 as a potential fish feed safety issue in the LVB and suggests a need for more research on mycotoxin residues in fish fillets.
Guidelines for endoscopic diagnosis of early gastric cancer

The Japan Gastroenterological Endoscopy Society developed the Guideline for Endoscopic Diagnosis of Early Gastric Cancer based on scientific methods. Endoscopy for the diagnosis of early gastric cancer has been acknowledged as a useful and highly precise examination, and its use has become increasingly more common in recent years. However, the level of evidence in this field is low, and it is often necessary to determine recommendations based on expert consensus only. This clinical practice guideline consists of the following sections to provide the current guideline: [I] Risk stratification of gastric cancer before endoscopic examination, [II] Detection of early gastric cancer, [III] Qualitative diagnosis of early gastric cancer, [IV] Diagnosis to choose the therapeutic strategy for gastric cancer, [V] Risk stratification after endoscopic examination, and [VI] Surveillance of early gastric cancer.
The roles of environmental variation and spatial distance in explaining diversity and biogeography of soil denitrifying communities in remote Tibetan wetlands.

The relative importance of local environments and dispersal limitation in shaping denitrifier community structure remains elusive. Here, we collected soils from 36 riverine, lacustrine and palustrine wetland sites on the remote Tibetan Plateau and characterized the soil denitrifier communities using high-throughput amplicon sequencing of the nirS and nirK genes. Results showed that the richness of nirS-type denitrifiers in riverine wetlands was significantly higher than that in lacustrine wetlands but not significantly different from that in palustrine wetlands. There was no clear distinction in nir community composition among the three kinds of wetlands. Irrespective of wetland types, the soil denitrification rate was positively related to the abundance, but not the α-diversity, of denitrifying communities. Soil moisture, carbon availability and soil temperature were the main determinants of diversity (OTU number) and abundance of nirS-type denitrifier community, while water TOC, soil NO3- and soil moisture were important in controlling nirK-type denitrifier diversity and abundance. The nirS community composition was influenced by water electrical conductivity, soil temperature and water depth, while the nirK community composition was affected by soil electrical conductivity. Spatial distance explained more variation in the nirS community composition than in the nirK community composition. Our findings highlight the importance of both environmental filtering and spatial distance in explaining diversity and biogeography of soil nir communities in remote and relatively undisturbed wetlands.
Design and Performance of Novel Self-Cleaning g-C3N4/PMMA/PUR Membranes

In the majority of photocatalytic applications, the photocatalyst is dispersed as a suspension of nanoparticles. The suspension provides a higher surface for the photocatalytic reaction in respect to immobilized photocatalysts. However, this implies that recovery of the particles by filtration or centrifugation is needed to collect and regenerate the photocatalyst. This complicates the regeneration process and, at the same time, leads to material loss and potential toxicity. In this work, a new nanofibrous membrane, g-C3N4/PMMA/PUR, was prepared by the fixation of exfoliated g-C3N4 to polyurethane nanofibers using thin layers of poly(methyl methacrylate) (PMMA). The optimal amount of PMMA was determined by measuring the adsorption and photocatalytic properties of g-C3N4/PMMA/PUR membranes (with a different PMMA content) in an aqueous solution of methylene blue. It was found that the prepared membranes were able to effectively adsorb and decompose methylene blue. On top of that, the membranes evinced a self-cleaning behavior, showing no coloration on their surfaces after contact with methylene blue, unlike in the case of unmodified fabric. After further treatment with H2O2, no decrease in photocatalytic activity was observed, indicating that the prepared membrane can also be easily regenerated. This study promises possibilities for the production of photocatalytic membranes and fabrics for both chemical and biological contaminant control.
Comparison of volatile organic compound profiles in exhaled breath versus plasma headspace in different diseases

Breath analysis is the study of volatile organic compounds (VOC’s) in exhaled breath. This analysis provides information on the body’s condition. In this study we investigated the relationship between 22 VOC’s detected in exhaled breath and plasma headspace using a selected ion flow tube mass spectrometer (SYFT-MS). We compared pairs of exhaled breath and plasma samples from patients with pulmonary hypertension inflammatory bowel disease (IBD), and IBD patients after J-pouch surgery (pouch group). Half of the measured VOC’s from exhaled breath were significantly associated with the VOC’s from plasma headspace. Interestingly, six breath VOC’s (trimethyl amine (FDR p = 0.02), hydrogen sulfide (FDR p = 7.64 × 10−30), ethanol (FDR p = 1.56 × 10−4), dimethyl sulfide (FDR p = 5.70 × 10−19), benzene (FDR p = 8.40 × 10−27), and acetaldehyde (FDR p = 4.27 × 10−17)) and two plasma headspace VOC’s (1-Octene (FDR p = 0.02) and 2-propanol (FDR p = 2.47 × 10−9)) were able to differentiate between the three groups. Breath and plasma headspace share a similar signature with significant association in half of the measured VOCs. The disease discriminatory capacity of breath and plasma headspace appear to be different. Therefore, using the VOC’s print from both breath and plasma headspace may better help diagnose patients.
Correction of a Malpositioned Central Venous Catheter using Point-of-care Transthoracic Echocardiography

© 2020 Annals of Cardiac Anaesthesia | Published by Wolters Kluwer Medknow This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution‐NonCommercial‐ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non‐commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Management Skill and Organizational Performance: Mediating Role of Self-Management in Nigeria

Drawing on the social exchange theory, this study aims to analyze the influence of management skill on organizational performance. Specifically, this study sought to investigate mediating role of self-management between management skill, and organizational performance by utilizing structural equation modeling (SEM) technique. Four propositions which explained the relationships among the variables in the study were postulated. In the context of the study, the introduced model was tested by a questionnaire instrument with 18 items, excluding the demographic variables in Nigeria. A total of 317 completed copies of questionnaires were evaluated for analysis. The findings of the study indicated positive correlations among the variables with high factor loadings. The results of this study also revealed that self-management mediated the relationship between management skill and organizational performance.
Presurgical Assessment and Surgical Treatment in Extra Temporal Lobe Epilepsy: A National Comprehensive Epilepsy Surgery Program in Cuba

This paper presents an Electro clinical outcome of Extra Temporal Lobe Epilepsy (ExTLE) patients derived from a national comprehensive epilepsy surgery program in Cuba. Twenty-three patients with pharma co resistant Ext TLE underwent thorough pre surgical evaluations as well as resective and disconnective surgical procedures tailored by sequential intra operative ElectroCorticography (ECoG). Seizure outcome assessment for each patient was carried out one and two years after seizure. Logistic regression analysis was used to consider the potential predictive factors, which included demographic, presurgical and surgical variables. Resective surgical techniques were performed on frontal, occipital, pericentral and parietal lobes. Hemispherectomy was implemented in one patient for Rasmussen encephalitis. Anterior callosotomies for drop attacks were completed in two children; and focal frontal resection was carried out in one patient additionally to anterior callosotomy. About 80.9% of the surgeries were done in non-dominant hemispheres. Furthermore, surgical resection encroaching upon eloquent cortex was accomplished in 42.8% of the Ext TLE patients. During presurgical evaluation, the most common seizure types were aware and non-aware focal seizure which evolved to bilateral tonic clonic seizures. Regional interictal Electroencephalography pattern was documented in 61.5% of the patients; while 66.6% exhibited non-lateralized or bilateral interictal epileptiform discharge. Ictal EEG pattern was lateralized in 69.2% and regional in 76.9% of the cohort. Magnetic Resonance Imaging did not indicate a distinct lesion in 52.1% of the cases. After one-year follow up, 52.6% was labeled (Engel class I) and 47.3% (class II-III); at 24 months: 41.1% cases were registered class I, and 58.8% class II-IV. Clinical evolutions did not show significant modification concerning the assessed terms (Friedman ANOVA p=0.15); and there was no variation in the number of cases registered Engel class I two years after surgical treatment compared to the previous year (p=0.47 Sign test). A drop in the Absolute Spike Frequency (ASF) was observed in the post resective intra operative ECoG performed in all resective surgeries. The ASF during pre-resection ECoG was lower in patients Engels Class I one and two years after surgery (*p<0.05, Mann U test). A multivariate logistic regression analysis demonstrated that an acute postoperative seizure was the sole postoperative variable related to Engel classification. (p=0.01), odd ratio 3.88. This multi-disciplinary and multi-modal program for presurgical epilepsy workup and surgical procedures guided by sequential intra operative ECoG indicates favorable outcomes that show a safe and viable procedure with mild morbidity, and no mortality.
Semantic Types of Some Generic Relation Arguments: Detection and Evaluation

There is disclosed a management system comprising an information management apparatus for managing maintenance information of image forming apparatuses, and a transmitter connected to the image forming apparatuses. In the management system, the transmitter transmits the maintenance information to the information management apparatus through a telephone line and an exchange unit, and a line controller connects the telephone to the telephone line so as to speak using the telephone normally, and further, cancels the connection of the telephone to the telephone line and connects the transmitter to the telephone line when the transmitter transmits the maintenance information to the information management apparatus.
The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.
Two genes involved in clindamycin resistance of Bacillus licheniformis and Bacillus paralicheniformis identified by comparative genomic analysis

We evaluated the minimum inhibitory concentrations of clindamycin and erythromycin toward 98 Bacillus licheniformis strains isolated from several types of fermented soybean foods manufactured in several districts of Korea. First, based on recent taxonomic standards for bacteria, the 98 strains were separated into 74 B. licheniformis strains and 24 B. paralicheniformis strains. Both species exhibited profiles of erythromycin resistance as an acquired characteristic. B. licheniformis strains exhibited acquired clindamycin resistance, while B. paralicheniformis strains showed unimodal clindamycin resistance, indicating an intrinsic characteristic. Comparative genomic analysis of five strains showing three different patterns of clindamycin and erythromycin resistance identified 23S rRNA (adenine 2058-N6)-dimethyltransferase gene ermC and spermidine acetyltransferase gene speG as candidates potentially involved in clindamycin resistance. Functional analysis of these genes using B. subtilis as a host showed that ermC contributes to cross-resistance to clindamycin and erythromycin, and speG confers resistance to clindamycin. ermC is located in the chromosomes of strains showing clindamycin and erythromycin resistance and no transposable element was identified in its flanking regions. The acquisition of ermC might be attributable to a homologous recombination. speG was identified in not only the five genome-analyzed strains but also eight strains randomly selected from the 98 test strains, and deletions in the structural gene or putative promoter region caused clindamycin sensitivity, which supports the finding that the clindamycin resistance of Bacillus species is an intrinsic property.
Genome-wide analysis of short interspersed nuclear elements provides insight into gene and genome evolution in citrus

Abstract Short interspersed nuclear elements (SINEs) are non-autonomous retrotransposons that are highly abundant, but not well annotated, in plant genomes. In this study, we identified 41,573 copies of SINEs in seven citrus genomes, including 11,275 full-length copies. The citrus SINEs were distributed among 12 families, with an average full-length rate of 0.27, and were dispersed throughout the chromosomes, preferentially in AT-rich areas. Approximately 18.4% of citrus SINEs were found in close proximity (≤1 kb upstream) to genes, indicating a significant enrichment of SINEs in promoter regions. Citrus SINEs promote gene and genome evolution by offering exons as well as splice sites and start and stop codons, creating novel genes and forming tandem and dispersed repeat structures. Comparative analysis of unique homologous SINE-containing loci (HSCLs) revealed chromosome rearrangements in sweet orange, pummelo, and mandarin, suggesting that unique HSCLs might be valuable for understanding chromosomal abnormalities. This study of SINEs provides us with new perspectives and new avenues by which to understand the evolution of citrus genes and genomes.
Developing a Topical Adjunct to Injectable Procedures.

Injectable procedures have come to play an enormous part in everyday aesthetic medical practice. Whether its use is directed at volumizing with fillers, decreasing volume using enzymes, skin-tightening using multi-needle approaches, or neuromuscular blockade, the injectable route is the means of delivery in all these cases, making injectable procedures the most common aesthetic procedure performed. As with all procedures, expected and unexpected consequences may follow including bruising, swelling, discomfort, and the possibility of infection. This paper outlines the scientific process and validation of a product designed as an adjunct to injection therapy and the scientific deep dive needed to encompass both symptomatic and adjunctive purposes. On the symptomatic side, bruising, swelling, and pain were considered, while volumetric enhancement, regeneration, and anti-microbial/biofilm effects were desired outcomes from the adjunctive perspective. Utilizing peptides and active agents aimed at reducing excess residual iron and stimulating macrophage absorption of red blood cells, we were able to achieve efficient resolution of bruising. In addition, peptides were included to stimulate collagen, elastin, and hyaluronic acid in synergy with the injectable. Anti-inflammatory, antimicrobial, and antibiofilm agents were added to aid in the safety profile of the injectable. In vivo testing of bruising resolution demonstrated that at day 2/3, participants using the study product (INhance Post-Injection Serum with TriHex Technology®, Alastin Skincare, Inc. Carlsbad, CA) had 73% less bruise color intensity and statistically significant improvement over the bland moisturizer. Overall, 81% of subjects applying the study topical product had less bruising at day 2/3 compared to the bland moisturizer. J Drugs Dermatol. 2020;19(4):398-404. doi:10.36849/JDD.2020. 5016.
Updated CDC Recommendations for Universal Hepatitis C Virus Screening Among Adults and Pregnant Women: Implications for Clinical Practice.

Acute hepatitis C virus (HCV) infections in the US have increased more than 3-fold since 2010.1 In 2017, there were an estimated 44 700 cases of acute HCV infection in the US,2 which is more than the 37 377 cases of HIV reported.3 In addition, there are now more deaths annually from HCV infection than from HIV in the US2,3; in 2017, HCV infection accounted for 17 253 deaths2 and HIV accounted for 15 971 deaths.3 Deaths related to HCV infection are considered to be largely preventable because therapies are now readily available that can achieve virological cure (defined as undetectable HCV infection in the blood for 12 weeks after completing treatment, and referred to as a sustained virological response). The identification and treatment of persons infected with HCV is imperative to prevent disease progression and associated morbidity and mortality, and to reduce HCV transmission. The development of newer and simpler treatment regimens means that all clinicians should be empowered to undertake treatment of patients with HCV infection in their practice because more than 95% of treatment-naive patients can achieve
Virulence Traits of Inpatient Campylobacter jejuni Isolates, and a Transcriptomic Approach to Identify Potential Genes Maintaining Intracellular Survival

There are still major gaps in our understanding of the bacterial factors that influence the outcomes of human Campylobacter jejuni infection. The aim of this study was to compare the virulence-associated features of 192 human C. jejuni strains isolated from hospitalized patients with diarrhoea (150/192, 78.1%), bloody diarrhoea (23/192, 11.9%), gastroenteritis (3/192, 1.6%), ulcerative colitis (3/192, 1.5%), and stomach ache (2/192, 1.0%). Traits were analysed with genotypic and phenotypic methods, including PCR and extracellular matrix protein (ECMP) binding, adhesion, and invasion capacities. Results were studied alongside patient symptoms, but no distinct links with them could be determined. Since the capacity of C. jejuni to invade host epithelial cells is one of its most enigmatic attributes, a high throughput transcriptomic analysis was performed in the third hour of internalization with a C. jejuni strain originally isolated from bloody diarrhoea. Characteristic groups of genes were significantly upregulated, outlining a survival strategy of internalized C. jejuni comprising genes related (1) to oxidative stress; (2) to a protective sheath formed by the capsule, LOS, N-, and O- glycosylation systems; (3) to dynamic metabolic activity supported by different translocases and the membrane-integrated component of the flagellar apparatus; and (4) to hitherto unknown genes.
Cardiac remodeling secondary to chronic volume overload is attenuated by a novel MMP9/2 blocking antibody

Objective Monoclonal antibody derivatives are promising drugs for the treatment of various diseases due to their high matrix metalloproteinases (MMP) active site specificity. We studied the effects of a novel antibody, SDS3, which specifically recognizes the mature active site of MMP9/2 during ventricular remodeling progression in a mouse model of chronic volume overload (VO). Methods VO was induced by creating an aortocaval fistula (ACF) in 10- to 12-week-old C57BL male mice. The VO-induced mice were treated with either vehicle control (PBS) or with SDS3 twice weekly by intraperitoneal (ip) injection. The relative changes in cardiac parameters between baseline (day 1) and end-point (day 30), were evaluated by echocardiography. The effects of SDS3 treatment on cardiac fibrosis, cardiomyocyte volume, and cardiac inflammation were tested by cardiac staining with Masson's trichrome, wheat Germ Agglutinin (WGA), and CD45, respectively. Serum levels of TNFα and IL-6 with and without SDS3 treatment were tested by ELISA. Results SDS3 significantly reduced cardiac dilatation, left ventricular (LV) mass, and cardiomyocyte hypertrophy compared to the vehicle treated animals. The antibody also reduced the heart-to-body weight ratio of the ACF animals to values comparable to those of the controls. Interestingly, the SDS3 group underwent significant reduction of cardiac inflammation and pro-inflammatory cytokine production, indicating a regulatory role for MMP9/2 in tissue remodeling, possibly by tumor necrosis factor alpha (TNFα) activation. In addition, significant changes in the expression of proteins related to mitochondrial function were observed in ACF animals, these changes were reversed following treatment with SDS3. Conclusion The data suggest that MMP9/2 blockage with SDS3 attenuates myocardial remodeling associated with chronic VO by three potential pathways: downregulating the extracellular matrix proteolytic cleavage, reducing the cardiac inflammatory responses, and preserving the cardiac mitochondrial structure and function.