Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunohistochemical localization of CuZn-superoxide dismutase in human uterine cervix and cervical cancer.

To elucidate the role of superoxide dismutase (SOD) in the uterine cervix and cervical cancer, we examined the immunohistochemical distribution of copper, zinc-superoxide dismutase (CuZn-SOD) in the human uterine cervix and cervical cancer. In the squamous epithelium, the basal, and the superficial, layers showed no or weak SOD imunoreactivity. In comparison, the prickle cell layer showed moderate immunoreactivity. In the columnar epithelium and cervical gland, ciliated cells showed moderate SOD immunoreactivity, whereas secretory cells showed no or weak reactivity. Subcolumnar reserve cells showed marked SOD immunoreactivity. These staining patterns were found to be constant throughout the menstrual cycle. In the carcinoma in situ, strong SOD immunoreactivity was seen in the cancer cells. In the invasive non-keratinizing and keratinizing squamous cell carcinomas, moderate immunoreactivity was seen. In the invasive adenocarcinoma of uterine cervix, marked immunoreactivity was seen in the well differentiated adenocarcinoma cells. On the other hand, no or weak immunoreactivity was seen in the moderately differentiated adenocarcinoma cells. Collectively, these results suggest that CuZn-SOD in uterine cervix might play an important role in the local defence mechanism against oxygen radicals and in the differentiation activity or reserve cells. Marked variability in CuZu-SOD immunoreactivity with various types of cervical cancers may reflect differences in cell proliferation kinetics. In the present survey of malignant tumors, we see no obvious relationship between generally assumed resistance to radiation or radical producing drugs and cellular immunoreactivity of CuZu-SOD. Futher studies are required to determine the value of CuZu-SOD analysis of tumors for the prediction of the response to radiation and redicalproducing drugs.
Quench-rate and size-dependent behaviour in glassy Ge2Sb2Te5 models simulated with a machine-learned Gaussian approximation potential

Phase-change memory materials are promising candidates for beyond-silicon, next-generation non-volatile-memory and neuromorphic-computing devices; the canonical such material is the chalcogenide semiconductor alloy Ge2Sb2Te5. Here, we describe the results of an analysis of glassy molecular-dynamics models of this material, as generated using a newly developed, linear-scaling (O(N)), machine-learned, Gaussian approximation potential. We investigate the behaviour of the glassy models as a function of different quench rates (varied by two orders of magnitude, down to 1 K ps−1) and model sizes (varied by two orders of magnitude, up to 24 300 atoms). It is found that the lowest quench rate studied (1 K ps−1) is comparable to the minimum cooling rate needed in order completely to vitrify the models on quenching from the melt.
Important advances in malaria vaccine research

Malaria is one of the most widespread parasitic infection in Asian countries affecting the poor of the poor. In an effort to develop an effective vaccine for the treatment of malaria, various attempts are being made worldwide. If successful, such a vaccine can be effective for treatment of both Plasmodium vivax and Plasmodium falciparum. This would also be able to avoid complications such as drug resistance, resistance to insecticides, nonadherence to the treatment schedule, and eventually high cost of treatment in the resource-limited settings. In the current compilation, the details from the literature were collected by using PubMed and Medline as search engines and searched for terms such as malaria, vaccine, and malaria treatment. This review collates and provides glimpses of the information on the recent malaria vaccine development. The reader will be taken through the historical perspective followed by the approaches to the malaria vaccine development from pre-erythrocytic stage vaccines, asexual stage vaccines, transmission blocking vaccines, etc. Looking at the current scenario of the malaria and treatment strategies, it is an absolute need of an hour that an effective malaria vaccine should be developed. This would bring a revolutionary breakthrough in the treatment modalities especially when there is increasing emergence of resistance to existing drug therapy. It would be of great purpose to serve those living in malaria endemic region and also for travelers which are nonimmune and coming to malaria endemic region. As infection by P. vivax is more prevalent in India and other Asian subcontinent and is often prominent in areas where elimination is being attempted, special consideration is required of the role of vaccines in blocking transmission, regardless of the stages being targeted. Development of vaccines is feasible but with the support of private sector and government organization in terms of regulatory and most importantly financially, being an expensive venture.
Design and Implementation of the UDS Diagnostic System Based on CAN Bus

Based on the ISO15765 protocol and the electronic control unit (ECU) to complete the design and implementation of host computer software for the fault diagnosis function. The main contents include: This paper first introduces the basic requirements and specifications of CAN bus and ISO15765 protocol, then introduces the specific requirements and definitions of all diagnostic service in the implementation process , Using CANoe software simulation method implements the diagnostic requirements. Finally, it introduces the core process and state transfer process of the software design . Test the software and compare it with the traditional version which is carried out through CANoe software and then analyze the result and draw a conclusion.
Hybrid functionals with system‐dependent parameters: Conceptual foundations and methodological developments

Approximate density‐functional theory (DFT) has become the major workhorse of modern computational chemistry and materials science, but the most widely used DFT approaches, local‐density approximation (LDA) and generalized gradient approximation (GGA), suffer from some fundamental deficiencies, including, in particular, the band gap problem. As a relatively cheap way to overcome the difficulty confronted by LDA/GGA, hybrid functional methods have attracted tremendous interest, first in molecular quantum chemistry, and more recently also in computational materials science. While early hybrid functionals use fixed parameters that are determined either by fitting some standard experimental database or based on theoretical arguments, recent studies have clearly indicated that the hybridization parameters carry on the physical significance and therefore should be system‐dependent. Developing theoretical methods to evaluate those parameters in a first‐principles manner has become one of the most active frontiers in theoretical chemistry community, and various schemes have been proposed. In this article, we aim at giving a systematic overview on the main theoretical concepts underlying various strategies and review major methodological developments in the recent years.
Returning to their roots: Examining the reintegration experiences of returned Indigenous migrant youth in Guatemala

This article examines the reintegration experiences of Indigenous migrant youth returned to Guatemala from the United States and Mexico, thereby filling a gap in extant literature. This qualitative study employed a critical ethnographic approach with reflexive thematic analysis of fieldwork and interviews with social service providers working with this population through the lens of Critical Latinx Indigeneities. Four major themes emerged: identity negotiation (sub-themes being Indigenous identities and returned migrant identities), trauma and its consequences, institutional and internalized oppression, and decolonization. Implications for social work emphasize the importance of Indigenous and decolonizing approaches to social work.
Molecular Phylogenetic Analysis of the Circumboreal Genus Cassiope (Ericaceae) Reveals Trends in Some Morphological and Wood Anatomy Characters and Likely Reticulate Evolution

Abstract The genus Cassiope is comprised of 17 species of dwarf alpine shrubs that exhibit a variety of leaf morphologies, yet have very similar flowers and habits. The genus is circumboreally distributed, with species diversity concentrated in northwestern North America and in eastern Asia. This clade has been previously shown to be strongly supported as sister to the subfamily Ericoideae, but relationships within the genus have never been the subject of any phylogenetic study. The goal of this study was to use three molecular markers from the chloroplast and nuclear genomes to generate a phylogeny of Cassiope. Our initial analyses suggested that three species negatively impacted phylogeny reconstruction by introducing conflict between the nuclear and chloroplast genomes. Reanalysis with those three taxa removed generated a very strongly supported phylogeny, suggesting that these three taxa may be of hybrid origin. Some evolutionary trends in morphology and anatomy are apparent based upon analyses using the restricted taxon sampling. Characters with discrete character states, such as leaf form and stomatal distribution, are tightly correlated but appear to be homoplasious within the genus. Continuously varying characters such as habitat elevation and aspects of wood anatomy show compelling phylogenetic trends that may be related to the fact that these species evolved in alpine and subalpine habitats. Cassiope appears to have a complex biogeographical history in the northern hemisphere, which cannot be completely resolved with the current dataset. However, it is likely that the genus has undergone multiple speciation events in the Himalayas with one or more dispersals into northwestern North America, or alternately with origins in the American Northwest followed by dispersal and subsequent divergence in the Himalayas.
A new species of the commercially harvested jellyfish Crambionella (Scyphozoa) from central Java, Indonesia with remarks on the fisheries

We describe a new species of Crambionella, C. helmbiru, from central Java, Indonesia. The combination of the mean number of lappets per octant (14), presence of foliaceous appendages amongst frills on oral-arms, absence of tubercles on the velar lappets, proportion of terminal club length to oral-arm length (0.28), and the body colour distinguish this species from three previously described congeners. In addition, the analysis of partial sequences of the cytochrome c oxidase subunit I gene indicate substantial genetic differences from both Crambionella orsini and Crambionella stuhlmanni, supporting the validity of this new species. A combination of morphological and genetic approaches determined that the remarkable differences in exumbrellar colours observed in specimens are simply intra-specific variation. Surprisingly, this species has been commercially harvested for more than 20 years and is well-known to the local people in the region, yet it had remained unknown to science until this point. The commercial fisheries targeting this formerly unknown species are also described in detail.
Effects of Groundwater Pumping on Ground Surface Temperature: A Regional Modeling Study in the North China Plain

Overexploitation of groundwater (GW) in the North China Plain (NCP) since the 1960s has many environmental consequences. However, mechanistic understanding of this perturbation remains limited, particularly at the regional scale. In this study, the coupled ParFlow.CLM model representing subsurface and land surface processes and their interactions was applied in the NCP at high spatiotemporal resolutions. The model was validated using the water and energy fluxes reported in previous studies and from the JRA‐55 reanalysis. Numerical experiments were designed to examine the impacts of GW pumping and irrigation on the ground surface temperature (GST). Results show significant effects of GW pumping on GST in the NCP. Generally, the subsurface acts as a buffer to temporal variations in heat fluxes at the land surface, but long‐term pumping can gradually weaken this buffer, leading to increases in the spatiotemporal variability of GST, as exemplified by hotter summers and colder winters. Considering that changes of water table depth (WTD) can significantly affect land surface heat fluxes when WTD ranges roughly between 0.01 and 10 m, the 0.5‐m/yr increase of WTD simulated by the model due to pumping can continue to increase the regional averaged WTD and hence, perturb GST for about 20 years after GW pumping began in the NCP, before WTD exceeds 10 m. The variations of GST are expected to increase faster initially and gradually slow down due to the nonlinear behaviors of GST with WTD. The findings from this study in the NCP may also have implications for other regions with GW depletion.
A comparison of internal phosphorus loads in lakes with anoxic hypolimnia: Laboratory incubation versus in situ hypolimnetic phosphorus accumulation1

Phosphorus release rates from anoxic sediments were determined from laboratory incubations of minimally disturbed sediment cores from seven lakes. These release rates were multiplied by an “anoxic factor,” based on the area and duration of hypolimnetic anoxia, to estimate internal phosphorus load. Despite individual variation, the experimentally derived internal loads were not significantly different from internal loads determined from hypolimnetic phosphorus increases at the end of summer stratification, except at internal loads <50 mg m−2 yr−1. Therefore, laboratory release rates can help predict internal loads after lake conditions have changed, e.g. after in-lake restoration measures.
Clinical Significance and Prognostic Value of Novel Echocardiographic Index for the Severity of Mitral Regurgitation

Background: Recently, the left ventricular early inflow-outflow index (LVEIO), calculated by dividing mitral E-wave velocity by the left ventricular outflow velocity time integral, has been proposed as a simple method for evaluating mitral regurgitation (MR). This study determined the optimal LVEIO threshold to assess severe MR with different etiologies and assessed its prognostic value. Methods and Results: The records of 18,692 consecutive patients who underwent echocardiography were reviewed. MR was classified into 4 groups: Grade 0/1, no, trivial, or mild MR; Grade 2, moderate MR; Grade 3, moderate to severe MR; and Grade 4, severe MR. The mean (±SD) LVEIO of Grades 0/1, 2, 3, and 4 was 3.6±1.4, 6.0±2.5, 7.4±3.1, and 9.5±2.8, respectively. An optimal LVEIO threshold of 5.4 was determined to distinguish moderate to severe or severe MR from non-severe MR (sensitivity 84%, specificity 91%). Kaplan-Meier survival analysis revealed high mortality in the group with LVEIO ≥5.4 (P=0.009, hazard ratio 1.833). This was found only in primary MR when separate analyses were performed according to etiology. Multivariate analysis revealed that LVEIO was an independent predictor for all-cause death only in primary MR. Conclusions: Using appropriate thresholds, LVEIO is a simple and useful method to diagnose severe MR regardless of etiology. LVEIO can also be useful for predicting prognosis in primary MR.
Growth Patterns of Jojoba Male and Female Leaf Callus with Various Ammonium and Nitrate Sources

Abstract Male and female leaf discs of Jojoba [Simmondsia chinensis (Link) Schneider] were cultured on Murashige and Skoog (MS) media supplemented with various nitrate:ammonium ratio and phytohormones concentrations. For the optimum callus growth, hormonal concentrations were remained equal for both male and female leaf tissues i.e., 0.4 mg L−1 2,4‐dichlorophenoxyaceticacid, 1.25 mg L−1 6‐benzyladenine and 0.5 mgL−1 kinetin. However, a statistically significant difference was observed when Murashige and Skoog media was supplemented with an additional nitrogen source. In female leaf tissue, maximum fresh and dry weights were recorded in Murashige and Skoog media supplemented with an additional source of NO3 −:NH4 + (60 mM) whereas in male leaf tissue this addition was inhibitory. This study suggests that nitrogen requirement may be different for optimum callus growth in both male and female leaf tissues.
Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase

Growth of Methanobacterium thermoautotrophicum strain Marburg was strongly inhibited by 2 μg/ml of pseudomonic acid. This inhibition was relieved by 10 mM l-isoleucine. The susceptibility of a pseudomonic acid-resistant mutant was 50 times lower than in the wild type. Isoleucyl-tRNA synthetase activity in cell extracts from the wild type was strongly inhibited by pseudomonic acid whereas the corresponding enzyme in mutant extracts was only marginally inhibited by the antibiotic.
Isolation and characterization of polymorphic microsatellite loci from the pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae)

Thirteen polymorphic microsatellite markers were developed from an enriched partial genomic library for the pink bollworm, Pectinophora gossypiella (Saunders), one of the most important cotton pests in the world. The total number of alleles ranged from two to 12 for a sample of 50 individuals and the expected heterozygosity at these loci ranged from 0.042 to 0.830. Although the presence of null alleles in some loci is suspected, these polymorphic markers are likely to provide useful tools for the population genetic studies of this species.
Effects of tree canopy on litter decomposition rates of Abies nordmanniana, Picea orientalis and Pinus sylvestris

Abstract Effects of tree canopy (distance from the stem) on litter decay rates were investigated in the field for 4 years. Needle litter of Nordmann fir [Abies nordmanniana (Stev.) Spach. ], Oriental spruce [Picea orientalis (L.) Link] and Scots pine (Pinus sylvestris L.) species were placed at three distances from their stems (<1 m, 1–2 m and >2 m). The litter showed highest decomposition rates at the distance of >2 m and lowest rates at <1 m for all species. Initial lignin concentrations were most strongly correlated with the decay rates, but among the microclimatic and soil factors, forest floor moisture content was the best predictor. The results showed that litter quality variables define the potential rates of microbial decomposition rates within and between trees, but adverse environmental conditions created by a tree canopy can also significantly affect decomposition rates. The closer distance to the stem significantly reduces litter decay rates by changing environmental conditions (especially forest floor moisture content) which can reduce microbial activity, and thus, the effects of tree canopy on litter decomposition rates should be taken into an account when calculating or comparing litter decomposition dynamics of forest ecosystems.
Intersectional discourses of reproductive agency in the Philippines: A mixed methods analysis of classed constructions of pregnancy resolution

Women negotiate gendered discourses of reproductive agency in resolving unplanned pregnancies. Invoking an intersectional lens, this paper examines how these discursive dynamics differentiate across social class in the Global South context of the Philippines. Utilizing a novel mixed methods strategy, we triangulate quantitative findings from a Q sort task and qualitative accounts of pregnancy resolution to identify classed discourses of reproductive agency as (a) reclaiming maternal virtue and (b) asserting autonomous choice. Statistical analysis reveals significant discursive divergence across class, wherein working-class women primarily adopt maternal virtue discourses while middle-class women disproportionately subscribe to autonomous choice. Interpretative analysis of women’s accounts complicates this bifurcated characterization by surfacing the diverse ways by which women negotiate both discourses in narratives of abortion and carrying to term. We discuss our contributions to the literature in terms of multilevel theoretical engagement with classed complexity in gendered issues like reproductive agency as well as innovating mixed methods in intersectional research. We conclude with reflections on advancing reproductive justice, especially in Global South societies like the Philippines.
Microencapsulated extracts from Azadirachta indica seeds: Acquisition, characterization, and use in controlling Helicoverpa armigera

Abstract Biodegradable insecticides of Azadirachta indica A. Juss (Meliaceae) can be sustainable alternatives for agriculture. The goal of this study was to develop spray-dried microencapsulated extracts from A. indica seeds and to evaluate their effects in vitro on Helicoverpa armigera Hübner. Five polymeric matrices based on different proportions of gum arabic and maltodextrin were produced and characterized by evaluating photostability profile, drying yield, encapsulation efficiency, water activity, and azadirachtin content. In the photostability study, sample 3 showed the lowest loss of azadirachtin at the end of 15 days (62.90%). In the bioassays, the deterrence percentage was 100%. It was observed that in a 15-day period there was 100% mortality among the caterpillars that had a diet containing about 288 to 576 μg. mL−1 of microencapsulated extract. The microencapsulated extract from A. indica seeds presented increased azadirachtin photostability and showed potential as a bioinsecticide in the control of H. armigera.
A method to transform the β‐lactam antibiotic producer Streptomyces wadayamensis

We have developed a transformation system for Streptomyces wadayamensis, a cephamycin C producer. 1−5 × 109 protoplasts can be obtained when late logarithmic phase cultures of this microorganisms are incubated with 10 mg/ml of lysozyme. Polyethylene glycol-Ca2+-mediated transformation of these protoplasts yielded 106 transformants per μg of pIJ702 or pIJ365 DNA.
Calcium dependence of the effects of abscisic acid on RNA synthesis during germination of Cicer arietinum seeds

The effect of abscisic acid (ABA) and CaCl2 in the medium on RNA synthesis in embryonic axes of seeds of chick-pea (Cicer arietinum L. cv. Castellana) during the first hours of germination has been studied. ABA decreases the incorporation of 3H-uridine in the embryonic axes and modifies the mRNA populations by preventing the disappearance of three polypeptides of 25, 22 and 21.6 kDa and inducing the appearance of two polypeptides of 35 and 27 kDa absent in the control. Calcium increases the effect of ABA on the synthesis of these mRNAs and seems to be involved in the synthesis of at least the polypeptide of 25 kDa, as this polypeptide disappears in the treatments with EGTA and Verapamil. Moreover, cytosolic calcium seems to be necessary for the accumulation of these messengers since treatment with TMB-8 (chelating agent for endogenous calcium) decreases the intensity of the bands corresponding to these 5 polypeptides. The possible synergistic action of ABA and calcium in this process is discussed.
Polymorphic microsatellites from silver crucian carp (Carassius auratus gibelio Bloch) and cross-amplification in common carp (Cyprinus carpio L.)

Fifteen polymorphic microsatellites were isolated from the genome of silver crucian carp (Carassius auratus gibelio Bloch). Allele numbers ranged from two to four with an average of 2.7/locus, and the proportion of tri- and diallelic heterozygotes was 99.3%. The individuals tested seem to have originated from two different clonal lines: 14 of 16 showed the same genotype at all loci tested, whereas the remaining two were also identical, but different from the former ones. Eleven out of 15 primer pairs cross-amplified products from the genome of common carp, whereas only five from that of zebrafish.