Miracle-dz/UniFAR-FTUs huggingface dataset:
Therapeutic Discovery Identification of Small-Molecule Inhibitors of the Colorectal Cancer Oncogene Kr € uppel-like Factor 5 Expression by Ultrahigh-Throughput Screening

The transcription factor Kr€ uppel-like factor 5 (KLF5) is primarily expressed in the proliferative zone of the mammalian intestinal epithelium, where it regulates cell proliferation. Studies showed that inhibition of KLF5 expression reduces proliferation rates in human colorectal cancer cells and intestinal tumor formation inmice. To identify chemical probes that decrease levels of KLF5, we used cell-based ultrahigh-throughput screening (uHTS) to test compounds in the public domain of NIH, the Molecular Libraries Probe Production Centers Network library. The primary screen involved luciferase assays in the DLD-1/pGL4.18hKLF5p cell line, which stably expressed a luciferase reporter driven by the human KLF5 promoter. A cytotoxicity counterscreen was done in the rat intestinal epithelial cell line, IEC-6. We identified 97 KLF5-selective compounds with EC50 < 10 mmol/L for KLF5 inhibition and EC50 > 10 mmol/L for IEC-6 cytotoxicity. The two most potent compounds, CIDs (PubChem Compound IDs) 439501 and 5951923, were further characterized on the basis of computational,Western blot, and cell viability analyses. Both of these compounds, and two newly synthesized structural analogs of CID 5951923, significantly reduced endogenous KLF5 protein levels and decreased viability of several colorectal cancer cell lines without any apparent impact on IEC-6 cells. Finally, when tested in the NCI-60 panel of human cancer cell lines, compound CID 5951923 was selectively active against colon cancer cells. Our results show the feasibility of uHTS in identifying novel compounds that inhibit colorectal cancer cell proliferation by targeting KLF5. Mol Cancer Ther; 10(11); 2043–51. 2011 AACR.
Oriented longitudinal media on glass substrates

This paper reports on the recent breakthrough of achieving high orientation ratio (OR) on directly textured glass substrates. We present a unique medium design combining seedlayer, underlayer, and an antiferromagnetically coupled (AFC) magnetic layer structure. Such a structure gives rise to a high OR and leads to significant improvement of recording performance. This technology has enabled the extension of glass disk media to ultra-high density longitudinal recording.
Nomenclature for factors of the HLA system, update May 2012

The following sequences have been submitted to the Nomenclature Committee since the April 2012 nomenclature update and, following agreed policy, have been assigned official allele designations (Marsh et al., 2010). Full details of all sequences will be published in a forthcoming report. Below are listed the newly assigned sequences (Table 1) and confirmations of previously reported sequences (Table 2). The accession number of each sequence is given, and these can be used to retrieve the sequence files from the EMBL, GenBank or DDBJ data libraries. Although accession numbers have been assigned by the data libraries and most sequences are already available, there is still the possibility that an author may not yet have allowed the sequence to be released; in such a case, you will have to contact the submitting author directly. Additional information pertaining to new sequences is often included in the publications describing these alleles; a listing of recent publications that describe new HLA sequences is given in Table 3. All new and confirmatory sequences should now be submitted directly to the WHO Nomenclature Committee for Factors of the HLA System via the IMGT/HLA database using the sequence submission tool provided (Robinson et al., 2000). The IMGT/ HLA database may be accessed via the World Wide Web at: www.ebi. ac.uk/imgt/hla.
Liquid-liquid equilibrium phase diagrams of new aqueous two-phase systems: Ucon 50-HB5100 + ammonium sulfate + water, Ucon 50-HB5100 + poly(vinyl alcohol) + water, Ucon 50-HB5100 + hydroxypropyl starch + water, and poly(ethylene glycol) 8000 + poly(vinyl alcohol) + water

The phase diagrams of Ucon 50-HB-5100 (Ucon) + ammonium sulfate (NH4)2SO4 + water, Ucon 50-HB-5100 + poly(vinyl alcohol) 10000 (PVA 10000) + water, Ucon 50-HB-5100 + hydroxypropyl starch (Reppal PES 100) + water, and poly(ethylene glycol) 8000 (PEG 8000)−PVA 10000 systems were determined at 22 °C. Measurements at 30 and 40 °C were also done for each Ucon−salt system. A linear regression was used to calculate the slope and standard deviation for each tie line. The standard deviations are in most situations lower than 0.1, and the tie-line slopes for each phase diagram vary within 5.5% around the medium value for all cases with the exception of the Ucon + (NH4)2SO4 + water system at 40 °C. The phase diagrams of the Ucon + (NH4)2SO4 + water system at different temperatures were shown to have a smaller amount of salt and polymer than conventional systems and to be thermoseparating, versatile, and particularly attractive, since phase formation is temperature-dependent. The phase diagrams of Ucon + PVA 10000 + .
Species and strain comparisons in the macromolecular binding of extremely low doses of [14C]benzene in rodents, using accelerator mass spectrometry.

The kinetics of macromolecular binding of a 5 micrograms/kg body wt dose of [14C]benzene was studied over 48 h in B6C3F1, DBA/2, and C57BL/6 mice and Fischer rats to determine if adduct levels reflect known differences in metabolic capacity, genotoxicity, and carcinogenic potency. Previous studies have suggested that differences in benzene toxicity among strains result from differences in metabolism. Rats and mice were administered [14C]benzene (i.p.), followed by removal of liver and bone marrow at time intervals up to 48 h postexposure. Protein and DNA were isolated and analyzed by accelerator mass spectrometry. Area under the curves for protein and DNA adducts in bone marrow were greatest in B6C3F1 mouse > DBA/2 mouse > C57BL/6 mouse > Fischer rat. These data are consistent with the hypothesis that metabolic capacity contributes to the difference in benzene's carcinogenicity among species. Additionally, these data suggest that target organ adduct levels correlate with tumorigenicity and thus may be indicative of an individuals risk.
Influence of β-linkages on the morphology and performance of DArP P3HT–PC61BM solar cells

Direct arylation polymerization (DArP) has emerged as a greener and more atom-efficient alternative to Stille polymerization. Despite the attractiveness of this method, DArP is known to produce β-linkages in polymers, which have β-protons available for activation. Here, we report the influence of the β-defect content in DArP poly(3-hexylthiophene) (P3HT) on the performance of bulk-heterojunction solar cells and the morphology of pristine polymers and their blends with PC61BM in thin films and compare with Stille P3HT containing 0% β-defects as a reference point. The optical and electrochemical properties as well as the hole mobilities of pristine polymers remain virtually the same when the amount of β-defects is limited to 0.75% or lower, as evidenced by UV–visible absorption spectra, cyclic voltammetry and space-charge-limited current (SCLC) mobility measurements. However, an increase of β-defect concentration to 1.41% significantly affects the oxidation onset, UV–visible absorption profile and hole mobility of P3HT. The key result of this study is that the photovoltaic performance of DArP P3HT with 0% β-defects is remarkably close to that of Stille P3HT, whereas the performance of DArP P3HT with 0–0.75% β-defects does not differ dramatically from that of Stille P3HT and could potentially be improved upon by individual optimization of the processing conditions.
The effect of preservation solutions UW and EC on purine concentration in rat kidney.

INTRODUCTION Ischemia/reperfusion injury in organ transplantation is a multifactor process that may lead to organ damage and primary graft dysfunction. Perfusion is a process which creates a possibility of graft injury. Preservation solutions are thought to diminish the ischemic injury and an appropriate choice of the solution should guarantee a better graft function and good prognosis for graft survival. The aim of the study was to examine the effect of preservation solutions University of Wisconsin (UW) and Euro-Collins (EC) on purine concentration in rat kidney. MATERIAL AND METHODS The study was carried out on Wistar rat kidneys divided into 3 groups: kidneys perfused with 0.9% NaCl (control group), kidneys perfused with UW preservation solution, kidneys perfused with EC preservation solution. After bilateral nephrectomy the right kidney was immediately frozen in liquid nitrogen and the left kidney was placed in 4 degrees C UW, EC or 0.9% NaCl solution for 24 hours. The concentrations of purine nucleotides were determined using high-performance liquid chromatography (HPLC) method. RESULTS The concentrations ofpurine nucleotides in renal tissue without cold ischemia did not differ significantly between rats perfused with 0.9% NaCl, UW and EC solutions. The tissue concentrations of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), total adenine nucleotides (TAN), guanosine monophosphate (GMP) and inosine monophosphate (IMP) were significantly increased, whereas the concentrations of adenosine (Ado), inosine (Ino), guanosine (Guo), hypoxanthine (Hyp) and xanthine (Xan) were significantly lower in rats perfused with EC solution in comparison to rats perfused with 0.9% NaCl. CONCLUSIONS Purine concentration profile in rat kidney reflects a protective influence of EC and UW solutions on high-energy nucleotides in conditions when their depletion might be harmful to graft function.
Considerations for Nonintrusive Efficiency Estimation of Inverter-Fed Induction Motors

There has been a considerable trend in the use of inverter supplies for induction motors over sinusoidal supplies due to the requirement of efficient control of these machines in many automated systems. However, the use of inverters introduces additional losses in the motor as a result of voltage and current harmonics which impact negatively on the motor efficiency. This paper provides some considerations for nonintrusive efficiency estimation of inverter-fed induction motors, focusing on the quantification of additional losses for different harmonic equivalent circuits and the effect of voltage and current harmonics on the performance of these motors. Based on the analysis of the existing efficiency estimation methods, recommended techniques are proposed for state-of-the-art methods. An application of such techniques is presented. The future trend of the efficiency estimation of inverter-fed induction motors is highlighted. The rotor speed estimation techniques are reviewed.
Arm Span to Height Ratio in Relation to Severity of Dyspnea

Background: Arm span is the closest physiologic measurement to standing height. Increased arm span to standing height ratio, which indicates a possible loss of height, due to aging, often results from osteoporosisrelated vertebral collapse or other causes. This has possible consequences on lung functions. Objective: To evaluate the effects of aging related loss of height to severity of dyspnea. Method: Patients visited pulmonary function test laboratoryat Baghdad teaching hospital outpatient clinic department from first of November 2009 to thirty first of December 2010 for preoperative assessment were recruited to participate in our study. All recruited subjects were evaluated with a clinical history and physical examination followed by measurements of arm span, standing height, weight, forced expiratory volume in one second (FEV1) and forced vital capacity (FVC). Patients 40 years old of age and older were included . Patients with chronic disabilities (cardiac, respiratory or systemic diseases) were excluded. Patients were classified according to their gender and Medical Research Council severity of dyspnea. Results: one hundred thirty one subjects aged 40 years and older were recruited for this study. Arm span to height ratio was significantly negatively correlated with FEV1/FVC ratio, FVC, and positively correlated with severity of dyspnea. Conclusion:There is a significant association between increased arm span to height ratio with increased severity of dyspnea. The role of arm span measurements in assessment of pulmonary function tests in older patients and the association between loss of height and dyspnea deserves further evaluation.
Sex-Specific Effects of Surface Instability on Drop Jump and Landing Biomechanics

Abstract This study investigated sex-specific effects of surface instability on kinetics and lower extremity kinematics during drop jumping and landing. Ground reaction forces as well as knee valgus and flexion angles were tested in 14 males (age: 23±2 years) and 14 females (age: 24±3 years) when jumping and landing on stable and unstable surfaces. Jump height was found to be significantly lower (9%, p<0.001) when drop jumps were performed on unstable vs. stable surface. Significantly higher peak ground reaction forces were observed when jumping was performed on unstable versus stable surfaces (5%, p=0.022). Regarding frontal plane kinematics during jumping and landing, knee valgus angles were higher on unstable compared to stable surfaces (19-32%, p<0.05). Additionally, at the onset of ground contact during landings, females showed higher knee valgus angles than males (222%, p=0.027). Sagittal plane kinematics indicated significantly smaller knee flexion angles (6–35%, p<0.05) when jumping and landing on unstable vs. stable surfaces. During drop jumps and landings, women showed smaller knee flexion angles at ground contact compared to men (27–33%, p<0.05). These findings imply that knee motion strategies were modified by surface instability and sex during drop jumps and landings.
Strategies for Generating Prolonged Functional Standing Using Intramuscular Stimulation or Intraspinal Microstimulation

Spinal cord injury (SCI) often results in the loss of the ability to stand. The goal of this study was to implement a functional electrical stimulation (FES) system for restoring prolonged periods of standing after SCI. For this purpose, we tested two control strategies: open-loop and closed-loop control, and two stimulation paradigms: non-interleaved intramuscular stimulation (IM-S) and interleaved intraspinal microstimulation (ISMS). The experiments were conducted in anesthetized cats. Stimulation was applied to the muscles through IM-S electrodes implanted in the main knee and ankle extensor muscles, or to the spinal cord through ultra-fine ISMS wires implanted within the ventral horn of the lumbosacral enlargement. The cats were partially supported over parallel force plates and accelerometers were secured to the hindlimbs above and below the ankle joint. Ground reaction forces and knee and ankle joint angles were measured by the force plates and accelerometers, respectively. The closed-loop controller used these feedback signals to modulate the amplitude of stimulation applied to the extensor muscles. The open-loop controller applied constant levels of stimulation which were determined before the onset of each trial. The duration of standing achieved using closed-loop control of IM-S was significantly longer than that achieved with open-loop control (~2 times longer). The increase in the duration of standing corresponded with a decrease in the rate of force decay and a lower average injected current during closed-loop control. Standing was further improved with the use of ISMS. Closed-loop control of interleaved ISMS resulted in a period of standing > 3 times longer than the best trial generated using non-interleaved IM-S. There was also a significant improvement in the balance of force between the two hindlimbs. The results suggest that a system which uses closed-loop control in conjunction with interleaved ISMS could achieve prolonged FES standing in people with SCI.
HOGG: Gabor and HOG-Based Human Detection

Aimed at the multi-scale feature of human body in visual surveillance, a new multi-scale and multi-orientation feature extraction algorithm for human detection is put forwarded in this paper. First, extract the multi-orientation multi-scale amplitude spectrum of human body using Gabor filter. Second, fuse the multi-orientation Gabor features which has the same scale information into a single feature in order to reduce the dimension of the features. Third, Histograms of Oriented Gradients of each fused image is extracted and combined as human picture features. Experimental results on PETS2006 and CAVIAR database show that the proposed algorithm has a better performance.
What Happens During a Join? Dissecting CPU and Memory Optimization Effects

textabstractPerformance of modern hardware increasingly depends on proper utilization of both the memory cache hierarchy and parallel execution possibilities in todays super-scalar CPUs. Recent database research has demonstrated that database system performance severely suffers from poor utilization of these resources. In previous work, we presented join algorithms that strongly accelerate large equi-join by tuning the memory access pattern to match the characteristics of the memory cache subsystem in the benchmark hardware. In order to make such algorithms applicable in database systems that run on a wide variety of platforms, we now present a calibration tool that automatically extracts the relevant parameters about the memory subsystem from any hardware. Exhaustive experiments with join-queries demonstrate how a database system equipped with this calibrator can automatically tune memory-conscious database algorithms to their optimal settings. Once memory access is optimized, CPU resource usage becomes crucial for database performance. We demonstrate how CPU resource usage can be improved by using appropriate implementation techniques. Join experiments with the Monet database system on various hardware platforms confirm that combining memory and CPU optimization can lead to almost an order of magnitude of performance improvement on modern processors.
A time based markov model for automatic position-dependent services in smart home

A smart home is likely in the near future. An important ingredient in an intelligent environment such as a home is automatic services, which means home system itself could know or predict what the inhabitant want to do, and so provide inhabitant the services automatically. Many researches reveal that most of the services in smart home are location-dependent so the automatic services must be built on the location awareness. In this paper we model inhabitant location pattern as a time based markov model (TMM). The simulation result shows that compared to the other models, the TMM has a set of benefits such as less time complexity, higher prediction accuracy and faster convergences rate. These benefits make TMM meets the requirements of automatic services in smart home.
Selective Suppression of NF-kBp65 in Hepatitis Virus-Infected Pregnant Women Manifesting Severe Liver Damage and High Mortality

Fulminant hepatitis in Asian pregnant women is generally caused by hepatitis E virus infection, and extremely high mortality is most common in them. Decreased cell-mediated immunity is considered a major cause of death in these cases, but what exactly influences decreased immunity and high mortality specifically during pregnancy is not known. We used electrophoretic mobility shift assays, immunoblotting, and immunohistochemical analysis to study the expression and DNA binding activity of NF-kB p50 and NF-kB p65 in pregnant fulminant hepatic failure (FHF) patients and compared them with their nonpregnant counterparts. In both PBMC and postmortem liver biopsy specimens the DNA-binding activity of NF-kB was very high in samples from pregnant FHF patients compared with those from nonpregnant women as well as pregnant women with acute viral hepatitis (AVH) without FHF. Further dissection of the NF-kB complex in supershift assays demonstrated complete absence of p65 in the NF-kB complex, which is formed by homodimerization of the p50 component in pregnant FHF patients. Western blotting and immunohistochemical analysis of the expression of p50 and p65 proteins both showed higher levels of p50 expression and a complete absence or a minimal expression of p65, indicating its nonparticipation in NF-kB-dependent transactivation in pregnant FHF patients. We suggest that the exclusion of p65 from the NF-kB transactivation complex seems to be a crucial step that may cause deregulated immunity and severe liver damage, leading to the death of the patient. Our findings provide a molecular basis, for developing novel therapeutic approaches.
Inference for generalized exponential distribution based on generalized order statistics

Estimation of a parameter of generalized exponential distribution (gexp) is obtained based on generalized order statistics. The maximum likelihood and Bayes methods are used for this purpose. Survival function and hazard rate are also computed. Estimation based on upper record values from generalized exponential distribution is obtained as a special case and compared by simulated data.
Surface-wave leakage from substrate integrated waveguide on grounded dielectric substrate

This paper presents the surface-wave leakage from a substrate integrated waveguide (SIW) on a grounded dielectric substrate, which propagates the TM0 mode at the lowest order. The hybrid generalized scattering matrix (GSM) formulation, describing the electromagnetric field inside the rectangular waveguide walls, combined with the finite-element method(FEM), accurately depicting the fields surrounding the microstrip and distributing alongside the infinitely extended grounded substrate, establishes a rigorous formulation to tackle the periodic structure. The resulting lowest order dispersion characteristics show very good agreement with those of the TE10 mode of an ideal rectangular waveguide, when the gap between the posted walls is approaching one thousandth posted wall's diameter. Careful investigation of the electromagnetic field in the plane parallel to the ground plane shows that the leakage is 1) a TM0 surface mode, and 2) significant near and below the cutoff frequency of the rectangular waveguide section of the SIW.
A Dialog-Based Intelligent Tutoring System for Practicing Battle Command Reasoning

Abstract : This Phase II Small Business Innovation Research (SBIR) developed a dialog-based intelligent tutoring system (ITS) for interactive self-training of battle command reasoning. The system, called "Automated Tutoring Environment for Command" (ATEC), adapted the dialog management capability from AutoTutor (a dialog-based tutor developed by Graesser and colleagues at the University of Memphis) and integrated it with a cognitive model-based instructional agent (using all Systems' iGEN cognitive agent framework). The ATEC system presents a battlefield situation and then initiates a dialog between a virtual mentor (instructional agent) and a student as they collaboratively discuss the situation. The virtual mentor poses questions, evaluates student responses, determines the sequence of questions, and ultimately assesses performance on the basis of the specificity of questioning and the depth of probing and hinting that is needed to adequately answer the questions. The results of the ATEC development effort showed some of the capabilities and limitations of tutorial dialog systems, and indicated areas for additional research and development.