Miracle-dz/UniFAR-FTUs huggingface dataset:
Proteomics-Derived Biomarker Panel Improves Diagnostic Precision to Classify Endometrioid and High-grade Serous Ovarian Carcinoma

Purpose: Ovarian carcinomas are a group of distinct diseases classified by histotypes. As histotype-specific treatment evolves, accurate classification will become critical for optimal precision medicine approaches. Experimental Design: To uncover differences between the two most common histotypes, high-grade serous (HGSC) and endometrioid carcinoma, we performed label-free quantitative proteomics on freshly frozen tumor tissues (HGSC, n = 10; endometrioid carcinoma, n = 10). Eight candidate protein biomarkers specific to endometrioid carcinoma were validated by IHC using tissue microarrays representing 361 cases of either endometrioid carcinoma or HGSC. Results: More than 500 proteins were differentially expressed (P < 0.05) between endometrioid carcinoma and HGSC tumor proteomes. A ranked set of 106 proteins was sufficient to correctly discriminate 90% of samples. IHC validated KIAA1324 as the most discriminatory novel biomarker for endometrioid carcinoma. An 8-marker panel was found to exhibit superior performance for discriminating endometrioid carcinoma from HGSC compared with the current standard of WT1 plus TP53 alone, improving the classification rate for HGSC from 90.7% to 99.2%. Endometrioid carcinoma–specific diagnostic markers such as PLCB1, KIAA1324, and SCGB2A1 were also significantly associated with favorable prognosis within endometrioid carcinoma suggesting biological heterogeneity within this histotype. Pathway analysis of proteomic data revealed differences between endometrioid carcinoma and HGSC pertaining to estrogen and interferon signalling. Conclusions: In summary, these findings support the use of multi-marker panels for the differential diagnosis of difficult cases resembling endometrioid carcinoma and HGSC.
Participatory governance and local government responsiveness: evidence from participation in politics on television in China

ABSTRACT Based on the data of cities in China from 2005 to 2015, this paper examines the impact of the sandwiching approach involving the media governance model on government responsiveness with a quasi-natural experiment of participation in politics on television (PPTV). The study found that PPTV can help strengthen government responsiveness. After a series of robustness tests, the conclusion is still valid. The heterogeneity analysis shows that PPTV can effectively enhance government responsiveness in technology, social security, and employment sectors. Furthermore, government responsiveness is more effectively enhanced by adopting PPTV in form of annual live broadcast activities with public rating and cooperation with print media. This policy has a stronger role in enhancing government responsiveness in cities with high levels of economic development and high government competition. Considering officials characters, the longer the tenure of the mayor and the secretary of the municipal party committee, the stronger the government responsiveness.
Virulence Determinants and Genetic Diversity of Yersinia Species Isolated from Retail Meat

Yersinia enterocolitica is an important foodborne pathogen, and the determination of its virulence factors and genetic diversity within the food chain could help understand the epidemiology of yersiniosis. The aim of the present study was to detect the prevalence, and characterize the virulence determinants and genetic diversity, of Yersinia species isolated from meat. A total of 330 samples of retailed beef (n = 150) and pork (n = 180) in Latvia were investigated with culture and molecular methods. Whole genome sequencing (WGS) was applied for the detection of virulence and genetic diversity. The antimicrobial resistance of pathogenic Y. enterocolitica isolates was detected in accordance with EUCAST. Yersinia species were isolated from 24% (79/330) of meats, and the prevalence of Y. enterocolitica in pork (24%, 44/180) was significantly higher (p < 0.05) than in beef (13%, 19/150). Y. enterocolitica pathogenic bioserovars 2/O:9 and 4/O:3 were isolated from pork samples (3%, 6/180). Only resistance to ampicillin was confirmed in Y. enterocolitica 4/O:3 and 2/O:9 isolates, but not in other antimicrobials. Major virulence determinants, including ail, inv, virF, ystA and myfA, were confirmed with WGS in Y. enterocolitica 2/O:9 and 4/O:3. MLST typing revealed 15 STs (sequence types) of Y. enterocolitica with ST12 and ST18, which were associated with pathogenic bioserovars. For Y. enterocolitica 1A, Y. kristensenii, Y. intermedia and Y. frederiksenii, novel STs were registered (ST680-688). The presence of virulence genes and genetic characteristics of certain Y. enterocolitica STs confirm the common knowledge that pork could be an important source of pathogenic Yersinia.
MiR-499a-5p promotes 5-FU resistance and the cell proliferation and migration through activating PI3K/Akt signaling by targeting PTEN in pancreatic cancer

Background Pancreatic cancer (PC) can be considered a representative cancer type of the human body. As demonstrated by some studies, microRNA (miR)-499 is dysregulated in various cancer types including PC, for which chemotherapy involving 5-fluorouracil (5-FU) has long been considered the first-line therapy. However, there are complex and comprehensive mechanisms related to 5-FU, which have not been fully elucidated. This study thus aimed to examine the molecular mechanisms of 5-FU resistance through miR-499a-5p in PC. Methods The expression of miR-499a-5p in PC was measured using quantitative polymerase chain reaction (PCR). MiR-499a-5p was examined in-vivo for its effects on the malignant phenotypes of PC cells. Results The results of the present study demonstrated miR-499a-5p to be upregulated in PC and 5-FU resistant PC tissues. According to in vitro assays in PC cells (PANC1/FR), miR-499a-5p was found to affect adenosine triphosphate (ATP) binding cassette subfamily B member 1 (P-gp), ATP binding cassette subfamily C member 1 (MRP1), and ATP binding cassette subfamily G member 2 (BCRP), thereby facilitating 5-FU resistance in PC cells. Functions assays indicated that suppressed miR-499a-5p expression inhibited the proliferation and migration of cells but facilitated apoptosis in the PC cell line; by contrast, miR-499a-5p overexpression triggered the inverse phenotypic changes of cells. Concerning the mechanisms involved, miR-499a-5p increased PI3K/Akt signaling by targeting phosphatase and tensin homolog (PTEN). Conclusions Taken together, these findings demonstrate that miR-499a-5p can be potentially applied to PC therapy.
Early Clinical Outcomes of the Morbidly Obese Patients Who Underwent Laparoscopic Sleeve Gastrectomy by Gastric Cancer Surgeons: the Analysis of Fifty Consecutive Cases

Purpose The number of bariatric surgeries performed at our tertiary hospital has gradually increased since the national health insurance began to cover their expenses in January of 2019. This study examined the early surgical outcomes of laparoscopic sleeve gastrectomy (LSG) performed by experienced gastric cancer surgeons. Materials and Methods We retrospectively reviewed and analyzed data from 50 patients who underwent LSG between November of 2018 and April of 2020 at the Asan Medical Center by 1 of 5 experienced surgeons each of whom performed approximately 100–300 cases of gastrectomy annually. The age, body mass index (BMI), weight, presence of comorbidities, operation time, hospital stay after surgery, postoperative complications, postoperative excess weight loss (EWL), and resolution of comorbidities were examined. Results The mean age, BMI, and weight were 37.29±9.77 years, 37.12 kg/m2, and 102.00 kg, respectively. The mean operation time and postoperative length of hospital stay were 109.59±35.88 and 5.06±1.20 days, respectively. Two patients (4.00%) had early postoperative complications and postoperative leakage; bleeding and stenosis were not reported. The EWL after 1 and 6 months of operation was 26.55% and 60.34%, respectively. The resolution of diabetes, hypertension, and dyslipidemia after 6 months of operation was 88.89%, 54.54%, and 50.00%, respectively. Conclusion LSG is safe and effective when performed by an experienced gastric cancer surgeon; however, a long-term follow-up of patients is required.
In silico Discovery of a New Potent Inhibitor for Sterol 14-alpha Demethylase as a Promising Antifungal Drug against Aspergillus fumigatus Infection

Aspergillus fumigatus is a dangerous opportunistic pathogen that causes severe consequences for human beings when its conidia are inhaled. Several inhibitory drugs have recently been suggested to eradicate these fungi by inhibiting the cytochrome P450 sterol 14-alpha demethylase B (CYP51B). These drugs are designed to exhibit high specificity to the heme that is incorporated in the active site of this enzyme. Though effective binding with heme can be achieved, administration of these drugs can be accompanied by variable risks to the user’s health. Series of in silico screenings were conducted to find out more eligible drug-like compounds to inhibit CYP51B-heme with fewer side effects on patients. Using stringent ZINCPharmer restrictions, seventeen compounds were found to have efficient binding to the heme group of CYP51B. Their effectiveness against CYP51B was tested using molecular docking, drug-likeness prediction, and molecular dynamics (MD) simulation. One compound (ZINC000015774018 or molecule-8) was found to inhibit the heme group with better drug-likeness than that found in the other sixteen drug-like compounds. MD simulations showed that this ligand introduced stabilized interactions with the targeted protein upon interacting with its heme and amino acid residues. Thus it may be used as a potent antifungal inhibitor against A. fumigatus.
An Overview on Low-Level Laser Therapy (LLLT) & Cooling Laser Therapy (C.L.T.) in Medical Engineering

Some essential methods for overcoming the skin problem during laser therapy are Low-Level Laser Therapy and Cooling Laser Therapy in cooling the most superficial layers of the skin. Although melanin absorption will result in heat production during laser exposure, cooling the epidermis can prevent its temperature elevation from exceeding the threshold for thermal injury. L.D.C. is a technique for laser cooling of small particles. Low-Level Laser Therapy (LLLT), consisting of Low-intensity radiation in specific wavelengths, has been discussed to trigger cellular proliferation and differentiation through molecular mechanic mechanisms. This work studied the propagation of the laser beam into biological tissue such as the brain and skin by the Monte Carlo method. What is generally accepted is that the energy density required is very small, and the transmit power is less than 0.6 Watts
A Rare Simultaneous Occurrence of Splenic and Pelvic Cavity Hydatid Cyst

Hydatid cyst is a significant health-threatening problem that can affect almost all organs, especially the lungs and the liver, but the possibility of its occurrence in organs such as the spleen or pelvic cavity is rare. Thus, simultaneous hydatid cysts in the spleen and pelvic cavity are probably very rare. Nevertheless, since hydatid cysts in different areas can cause various symptoms, it should be considered a significant diagnosis. This article presents a case report of a 21-year-old woman presenting with right lower quadrant abdominal pain mimicking appendicitis but found to have simultaneous hydatid cysts in the spleen and the pelvic cavity.
Intercultural Communicative Competence Levels of Indonesian EFL Students: A Preliminary Study in a Higher Education Contex

The lack of intercultural communication skills will likely cause disharmony, misunderstanding, and even conflict in communication. To be successful in communication with native speakers depends on language skills, customs, and cultural knowledge. In the age of global communication, English as a Foreign Language (EFL) teaching goals should be reoriented to cultivate intercultural communication competence (ICC). EFL learners should have this intercultural competence and be sensitive in order to avoid disharmony, misunderstandings, and even conflicts in communication. The main objective of this preliminary study is to reveal the levels of intercultural communicative competence among EFL students at a state university in Indonesia. A survey questionnaire was performed employing a quantitative analysis in this study. Eighty-nine students filled out the ICC questionnaire, which consisted of 20 questions. Findings indicated that most of the students had a low ICC level because they are lack of experience and knowledge in interacting and socializing with people from various cultural backgrounds. In terms of gender differences, the results showed that male students had higher levels of ICC than that female students. This research implied that intercultural topics should be included in the university’s curriculum.
Unsupervised Multi-Look SAR Image Change Detection Driven by Dual-Layer Graph With Intensity and Texture Attributes

This letter proposes a novel graph-guided approach for unsupervised change detection between multi-look synthetic aperture radar (SAR) images. The dual-layer graph (DG) is proposed to combine both the intensity and texture attributes of an SAR image. It can not only preserve the characteristics of each attribute in the single layer, but also excavate the multiple affinities of attributes from different layers. With the support of DG, change measure is achieved through comparing the graphs based on the supra-adjacency matrix, thus generating the difference image with good separability. The final change detection results are obtained via a traditional binary classification algorithm. Experimental results on two real SAR datasets demonstrate the effectiveness of the proposed graph-guided approach in which DG and multiple affinities provide great contributions for improving the accuracy and robustness of change detection.
Magnetic field monitoring in submersible motor under eccentricity fault considering slotting effect

Large submersible motors make a huge contribution to national security prevention and control of water. But the eccentricity fault has a strong impact on the operational reliability. Therefore, this paper uses new diagnostic signals of air-gap magnetic field to detect eccentricity fault in submersible motor. Compared with the previous papers, this paper combines the effects of eccentricity and slotting. Firstly, a new air-gap permeance model is established considering slotting effect aimed at eccentric motor. Then the time harmonics of air-gap magnetic field are obtained based on MMF and permeance wave approach. Compared with healthy motor, the characteristic frequencies are chosen as diagnostic signals. The air-gap magnetic field is measured by search coil placed around stator tooth. The way is applied to detecting dynamic eccentricity and mixed eccentricity. Finally, for validating the theoretical results, the induced voltages of search coil are gained under different conditions in high voltage submersible motor by finite element method. The simulation is consistent with the theory in the process of data analysis.
A Comparative Study between Silicon Germanium and Germanium Solar Cells by Numerical Simulation

This paper presents the performance between silicon germanium (SiGe) and crystalline germanium (Ge) solar cells in terms of their simulated open circuit voltage, short circuit current density, fill factor and efficiency. The PC1D solar cell modeling software has been used to simulate and analyze the performance for both solar cells, and the total thickness is limited to 1μm of both SiGe and Ge solar cells. The Si0.1Ge0. 9 thickness is varied from 10nm to 100nm to examine the effect of Si0.1Ge0. 9 thickness on SiGe solar cell. The result of simulation exhibits the SiGe solar cell give a better performance compared to Ge solar cell. The efficiency of 9.74% (VOC = 0.48V, JSC = 27.86mA/cm2, FF =0.73) is achieved with Si0.1Ge0. 9 layer of 0. 1μm in thickness whilst 2.73% (VOC = 0.20V, JSC = 27.31mA/cm2, FF =0.50) efficiency is obtained from Ge solar cell.
Fennel (Foeniculum vulgare) and Ajwain (Trachyspermum ammi) extracts as potential preservatives in processed cheese foods

The study was conducted to evaluate the possibility of utilization of fennel and ajwain extracts as natural preservatives in processed cheese foods. Processed cheese foods were prepared with non dairy ingredients of potato, peanuts and inulin separately and treated with fennel and ajwain extracts. The level of extract addition was optimized to 1.2% total solids and based on sensory liking, fennel extract was added into potato and inulin added cheeses and ajwain extract was added to peanut added cheese. The effect of these extracts on the sensory, physicochemical and microbiological characteristics of processed cheese foods during storage under refrigerated (7-8 °C) conditions was evaluated. No significant (p > 0.05) change was observed in the sensory parameters of all the samples during storage, however the flavour was better maintained in extract added cheeses. A signiûcant (p < 0.05) effect was observed on the tyrosine content and free fatty acids as the extract added cheeses exhibited signiûcantly (p < 0.05) lower tyrosine and free fatty acid values. Electrophoretic study indicated lower protein degradation in spice extract added samples compared to those without extracts. A signiûcant (p < 0.05) effect was also observed on the microbiological characteristics of the products as treated samples showed signiûcantly (p < 0.05) lower values for yeast and mold count. Coliforms were not detected throughout the storage period. Fennel and ajwain extracts successfully improved the storage quality of processed cheese foods and may be commercially exploited as natural preservatives in cheese foods.
Mixed waste landfill corrective measures study final report Sandia National Laboratories, Albuquerque, New Mexico.

The Mixed Waste Landfill occupies 2.6 acres in the north-central portion of Technical Area 3 at Sandia National Laboratories, Albuquerque, New Mexico. The landfill accepted low-level radioactive and mixed waste from March 1959 to December 1988. This report represents the Corrective Measures Study that has been conducted for the Mixed Waste Landfill. The purpose of the study was to identify, develop, and evaluate corrective measures alternatives and recommend the corrective measure(s) to be taken at the site. Based upon detailed evaluation and risk assessment using guidance provided by the U.S. Environmental Protection Agency and the New Mexico Environment Department, the U.S. Department of Energy and Sandia National Laboratories recommend that a vegetative soil cover be deployed as the preferred corrective measure for the Mixed Waste Landfill. The cover would be of sufficient thickness to store precipitation, minimize infiltration and deep percolation, support a healthy vegetative community, and perform with minimal maintenance by emulating the natural analogue ecosystem. There would be no intrusive remedial activities at the site and therefore no potential for exposure to the waste. This alternative poses minimal risk to site workers implementing institutional controls associated with long-term environmental monitoring as well as routine maintenance and surveillance ofmore » the site. « less
Techno-Pedagogical Skills for 21st Century Digital Classrooms: An Extensive Literature Review

Techno-pedagogical ability is a way and reasonable value education available to everyone. Indeed, although in the past it has been found that there are many benefits to using innovation in the classroom environment, there are still obstacles or experiences related to the use of innovation. The literature review was utilized to gather and choose the papers for this research. This article investigates the techno-pedagogical skills requirements of the science and art teachers of the Sukkur State Government College. In this paper, literature is collected from different real sites, such as Google, Google Scholar, Science Direct (Elsevier), Sage, Springer, Emerald, Taylor & Francis, and Eric databases. Ten themes emerged from the literature to analyze the techno-pedagogical skills of science and art educators and provide suggestions and solutions for improving educational institutions. Quantitative, qualitative, and mixed research papers are all included in this study. This research also identified the knowledge gap and methodological and implication gap in this research article as per the need for 21st Century Digital Classrooms. The results show that there is no significant difference in techno-pedagogical skills between science teachers and art teachers who use technology in teaching. This is because of the epidemic. Talking about the current scourge, COVID-19 has transformed traditional courses into digitization through ICT integration. Information and Communication Technology (ICT) is seen as a tool that can help schools adapt to the transition from industry to information. It is also considered as a tool to provide, support, and strengthen educational reforms in accordance with the educational needs of the information society.
Prediction of confined flow field around a circular cylinder and its force based on convolution neural network

In this paper, a large number of numerical simulations of the external confined flow field around a circular cylinder is conducted by using the open source library OpenLB. The boundary information of the flow field is defined by Euclidean distance function. Then, massive data, including the velocity field and the drag and lift coefficients of the cylinder, are obtained and used for model training. Subsequently, two convolutional neural network models are presented. The input ends of the two models are the boundary information of the flow field, and the output ends are the velocity field and the force on the cylinder respectively. Once the two models are established, they are used to predict the velocity and force respectively. The predicted results are in good agreement with the real values. It turns out that the convolution neural network models can be used to predict the flow field and force with complex boundaries, and it has the advantages of high accuracy and efficiency in practical engineering applications.
New Insights on Plasmin Long Term Stability and the Mechanism of Its Activity Inhibition Analyzed by Quartz Crystal Microbalance

We used the research quartz crystal microbalance (RQCM) to monitor regulatory effects of plasmin and trypsin in the presence of their inhibitor α2-antiplasmin. The gold surface of quartz crystals was modified with a β-casein layer that served as a substrate for protease digestion. The addition of plasmin or trypsin as well as their mixtures with α2-antiplasmin resulted in an increase of resonant frequency, f, and in a decrease of motional resistance, Rm, depending on the molar ratio of protease: antiplasmin. At equimolar concentrations of protease and α2-antiplasmin (5 nM:5 nM) full inhibition of protease activity took place. Monitoring of plasmin activity on an hourly and daily basis revealed a prominent effect of autolysis and decrease of plasmin activity in freshly activated samples. The degree of inhibition as well as plasmin half-life (t1/2 = 2.48 ± 0.28 days) connected with its degradation was determined.
Source location in plates by using wavelet transform of AE signals

The accuracy of source location in plates or plate-like structures is greatly affected by the dispersion characteristics of acoustic emission(AE) waves. In order to improve the accuracy, the detected AE signals were pre-processed by using wavelet transform to be decomposed into the low frequency flexural wave and the high frequency extensional wave. Since the flexural wave is highly dispersive, a decomposed flexural component has been extracted for a single frequency or within a very narrow frequency band by inverse wavelet transform. The calculation of arrival time differences was based on the threshold crossing time. The experimental corroboration has been made for an A16061 plate and a CFRP laminate.