Miracle-dz/UniFAR-FTUs huggingface dataset:
Genes and elements involved in the regulation of the nervous system and growth affect the development of spinal deformity in Cyprinus carpio

Spinal deformity is a serious economic and animal welfare problem in intensive fish farming systems, which will be a significant unsolved problem for the fish sector. The aim of this study was to determine the relative expression of genes (Akt1 substrate 1, Calreticulin, Collagen type I alpha 2 chain, Corticotropin-releasing hormone, Chromodomain-Helicase DNA-binding, Growth hormone, Insulin like growth factor 1, Myostatin, Sine oculis-related homeobox 3, Toll-like receptor 2) in different tissues associated with spinal deformity and to determine the macroelement (calcium, magnesium, phosphorus, potassium, sodium, sulfur) and microelement (barium, copper, iron, manganese, strontium, zinc) content of spine in healthy and deformed common carps (Cyprinus carpio) in Hungary. The mRNA levels of the genes were measured in 7 different tissues (abdominal fat, blood, brain, dorsal muscle, genitals, heart, liver) by qRT-PCR. Correlations between gene expression and element content were analyzed by using linear regression and Spearman rank correlation. In a total of 15 cases, we found a statistically significant connection between gene expression in a tissue and the macro- or microelement content of the spine. In these contexts, the genes Akt1 substrate 1 (3), Collagen type I alpha 2 chain (2), Corticotropin-releasing hormone (4), Insulin-like growth factor 1 (4), and Myostatin (2), the tissue’s blood (3), brain (6), heart (5), and liver (1), the macroelements sodium (4), magnesium (4), phosphorus (1) and sulfur (2) as well as the microelement iron (4) were involved. We also found statistically significant mRNA level differences between healthy and deformed common carps in tissues that were not directly affected by the deformation. Based on our results, genes regulating the nervous system and growth, elements, and tissues are the most associated components in the phenomenon of spinal deformity. With our study, we wish to give direction to and momentum for the exploration of these complex processes.
Impact of covid-19 on long-term oxygen therapy 2020: A nationwide study in Sweden

Introduction Covid-19 can cause chronic hypoxic respiratory failure, but the impact on the need for long-term oxygen therapy (LTOT) is unknown. The aim was to investigate change in incidence and characteristics of patients starting LTOT in Sweden 2020 after the outbreak of the pandemic. Material and methods Population-based observational study using data from the National Registry for Respiratory Failure (Swedevox) and from a survey to all centres prescribing LTOT in Sweden. Swedevox data provided information on incidence of LTOT and characteristics of patients starting LTOT during 2015–2020. Results Between March-Dec 2020, 131 patients started LTOT due to covid-19, corresponding to 20.5% of incident LTOT in Sweden. Compared with 2015–19, the total number of patients starting LTOT did not increase. No significant differences in patient characteristics or underlying causes of hypoxemia were found between patients starting LTOT during 2020 compared 2015–2019. The majority of the LTOT centres estimated that, since the start of the pandemic, the incidence of LTOT was unchanged and the time devoted for LTOT work was the same or slightly less. Conclusions Covid-19 caused one fifth of all LTOT starts during the pandemic in 2020. The LTOT incidence overall did not increase possibly due to reduction in other infections.
Linearly Tunable RF-MEMS Capacitors Implemented Using An Integrated Removable Self-Masking Technique

WILSON, JOHN MICHAEL. Linearly Tunable RF-MEMS Capacitors Implemented Using an Integrated Removable Self-Masking Technique (Under the direction of Paul D. Franzon.) MEMS tunable capacitors exhibit a non-linear capacitance verses voltage tuning characteristic. In addition, electro-statically actuated devices suffer from ‘pull-in’ after exceeding 33% of their total displacement. This non-linear behavior and limitation on continuous tuning that occurs from ‘pull-in’, limits the easy-of-use and tuning range of previously reported MEMS tunable capacitors. This dissertation presents a MEMS tunable capacitor that produces a wide tuning range with linear tuning throughout. The basic concept is similar to that of trimming/tuning capacitors used in early radios, where multiple metal plates create a capacitor that is varied by rotating a shaft which changes the overlap area. However, this device is built using modern IC processing methods that enable batch fabrication. The core of the design comes from high yield, mechanically proven gear structures defined in the SUMMiT design library, available from Sandia National Labs. Significant alterations were made to the physical gear structure to realize the final device. A novel masking technique that enables the complex patterning of metal(s), in different amounts, on any layer(s) of a released chip was also conceived. This ‘integrated removable self-masking technique’ enables the construction of low-loss controlled impedance structures such as coplanar waveguides in a polysilicon only MEMS process. In addition, this technique allows metal to be deposited in a regulated manner on multiple layers of a post release chip so that low-loss metal-insulator-metal capacitors can be built. Numerous device topologies are possible each with advantages and disadvantages. These various topologies and their design are discussed in detail. The metallization techniques for depositing single or dual metal layers are presented, along with a discussion on the construction of the masking layers so that they can be easily removed. Device simulation, modeling, and measurement are then presented. LINEARLY TUNABLE RF-MEMS CAPACITORS IMPLEMENTED USING AN INTEGRATED REMOVABLE SELF-MASKING TECHNIQUE by JOHN MICHAEL WILSON A dissertation submitted to the Graduate Faculty of North Carolina State University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy ELECTRICAL ENGINEERING
Modelling of a non-linear coil with loss in iron using the Runge-Kutta methods

This work presents a study on dynamics of a circuit with a non-linear coil, where loss in iron is also taken into account. A coil model is derived using a state space description. The work also includes the development of an application in C# for coil dynamics examination, where the implicit RADAU IIA method of various orders is applied for the purpose of solving non-linear differential equations modelling the non-linear coil with loss in iron.
What do women at high risk of breast cancer request of a patient education day? Focus interviews with women before and after deciding about prophylactic interventions

Abstract Objective At a Danish Hospital, we wished to establish a co‐designed patient education day about prophylactic interventions for women at high risk of developing breast cancer. However, knowledge is lacking on the women's acceptability and requests for content. The objective of this study is to gain knowledge about the acceptability and requests of the content of a patient education day among women at high risk of breast cancer considering prophylactic mastectomy. Methods A user panel consisting of patients and health care professionals developed an interview guide for two focus interviews with two groups of women at high risk of breast cancer; one group had received a prophylactic mastectomy and one group considered it. Thematic analysis was used to explore the participants' acceptability and requests for content. Results Meaningful content was knowledge about prophylactic interventions, how to share knowledge with partners and children, and talking to equals in a safe forum. Not all participants wished to discuss own surgery in a group setting. Conclusion An education day is an acceptable and supportive format for gaining knowledge about surgery, but since some topics may be vulnerable to discuss in a group setting to some women, we suggest the education day as a valuable supplement to the individual consultations.
Noninvasive Prenatal Test Results Indicative of Maternal Malignancies: A Nationwide Genetic and Clinical Follow-Up Study

PURPOSE: Noninvasive prenatal testing (NIPT) for fetal aneuploidy screening using cell-free DNA derived from maternal plasma can incidentally raise suspicion for cancer. Diagnostic routing after malignancy suspicious–NIPT faces many challenges. Here, we detail malignancy suspicious–NIPT cases, and describe the clinical characteristics, chromosomal aberrations, and diagnostic routing of the patients with a confirmed malignancy. Clinical lessons can be learned from our experience. METHODS: Patients with NIPT results indicative of a malignancy referred for tumor screening between April 2017 and April 2020 were retrospectively included from a Dutch nationwide NIPT implementation study, TRIDENT-2. NIPT profiles from patients with confirmed malignancies were reviewed, and the pattern of chromosomal aberrations related to tumor type was analyzed. We evaluated the diagnostic contribution of clinical and genetic examinations. RESULTS: Malignancy suspicious–NIPT results were reported in 0.03% after genome-wide NIPT, and malignancies confirmed in 16 patients (16/48, 33.3%). Multiple chromosomal aberrations were seen in 23 of 48 patients with genome-wide NIPT, and a malignancy was confirmed in 16 patients (16/23, 69.6%). After targeted NIPT, 0.005% malignancy suspicious–NIPT results were reported, in 2/3 patients a malignancy was confirmed. Different tumor types and stages were diagnosed, predominantly hematologic malignancies (12/18). NIPT data showed recurrent gains and losses in primary mediastinal B-cell lymphomas and classic Hodgkin lymphomas. Magnetic resonance imaging and computed tomography were most informative in diagnosing the malignancy. CONCLUSION: In 231,896 pregnant women, a low percentage (0.02%) of NIPT results were assessed as indicative of a maternal malignancy. However, when multiple chromosomal aberrations were found, the risk of a confirmed malignancy was considerably high. Referral for extensive oncologic examination is recommended, and may be guided by tumor-specific hallmarks in the NIPT profile.
PD-1 cooperates with AIRE-mediated tolerance to prevent lethal autoimmune disease

Significance A variety of mechanisms safeguard the body from autoimmune reactions, yet how these processes cooperate is largely unclear. Using a range of mouse genetic models, we uncover a critical tolerogenic axis between the autoimmune regulator, AIRE, and the immune checkpoint molecule, PD-1. Their combined loss induced an early-onset lethal autoimmune syndrome driven by autoreactive CD4+ T cells that could not be restrained by regulatory T cells. These data shed light on how central and peripheral tolerance mechanisms work together, and highlight thymic function as a potentially key modifier of responses to anti–PD-1 therapies.
Circular RNA hsa_circ_0011298 enhances Taxol resistance of non‐small cell lung cancer by regulating miR‐486‐3p/CRABP2 axis

Circular RNAs (circRNAs) serve as critical regulators in the chemoresistance of human cancers, including non‐small cell lung cancer (NSCLC). We aimed to explore the role of hsa_circ_0011298 (circ_0011298) and its mechanism in Taxol resistance of NSCLC.
The Fim and FhaB adhesins play a crucial role in nasal cavity infection and Bordetella pertussis transmission in a novel mouse catarrhal infection model

Pulmonary infections caused by Bordetella pertussis used to be the prime cause of infant mortality in the pre-vaccine era and mouse models of pertussis pneumonia served in characterization of B. pertussis virulence mechanisms. However, the biologically most relevant catarrhal disease stage and B. pertussis transmission has not been adequately reproduced in adult mice due to limited proliferation of the human-adapted pathogen on murine nasopharyngeal mucosa. We used immunodeficient C57BL/6J MyD88 KO mice to achieve B. pertussis proliferation to human-like high counts of 108 viable bacteria per nasal cavity to elicit rhinosinusitis accompanied by robust shedding and transmission of B. pertussis bacteria to adult co-housed MyD88 KO mice. Experiments with a comprehensive set of B. pertussis mutants revealed that pertussis toxin, adenylate cyclase toxin-hemolysin, the T3SS effector BteA/BopC and several other known virulence factors were dispensable for nasal cavity infection and B. pertussis transmission in the immunocompromised MyD88 KO mice. In contrast, mutants lacking the filamentous hemagglutinin (FhaB) or fimbriae (Fim) adhesins infected the nasal cavity poorly, shed at low levels and failed to productively infect co-housed MyD88 KO or C57BL/6J mice. FhaB and fimbriae thus appear to play a critical role in B. pertussis transmission. The here-described novel murine model of B. pertussis-induced nasal catarrh opens the way to genetic dissection of host mechanisms involved in B. pertussis shedding and to validation of key bacterial transmission factors that ought to be targeted by future pertussis vaccines.
Comparison of the osseointegration of implants placed in areas grafted with HA/TCP and native bone

This study evaluated the osseointegration of implants in areas grafted with biphasic ceramic based on hydroxyapatite/β‐tricalcium phosphate (HA/TCP) and in native bone (NB). Twenty‐eight rats were randomly assigned into two groups of 14 animals each: HA/TCP group: implants installed in areas grafted with HA/TCP and NB group: implants installed in areas of native bone. Bone defects were made in both tibiae of the rats belonging to the HA/TCP group and then filled with this bone substitute. After 60 days, the rats were submitted to surgical procedures for implant placement in grafted areas in both tibiae in the HA/TCP group while the implants were installed directly in native bone in the NB group. The animals were euthanized 15 and 45 days, respectively, after the implant placement. Biomechanical (removal torque), microtomographic (volume of mineralized tissues around the implants), and histomorphometric (Bone‐Implant contact—%BIC and bone area between the implant threads—%BBT) analyzes were conducted to assess the osseointegration process. The HA/TCP group showed lower values of removal torque, volume of mineralized tissue around the implants, lower %BIC, and %BBT compared to the NB group in both experimental periods. Osseointegration of implants placed in grafted areas with HA/TCP was lower compared to the osseointegration observed in native bone areas.
Systematic Review of Incidence, Prevalence, and Trends in Health Outcomes for Australian and New Zealand Paramedics

Abstract Introduction The paramedic role carries inherent risk to practitioner health, due to a combination of work characteristics and the employment practices of different organizations. Emerging evidence suggests that paramedics worldwide may face a range of negative health outcomes. The purpose of this article was to systematically review the literature of paramedic health outcomes in the Australian and New Zealand context. Methods A systematic search of key databases and gray literature was conducted to identify all available studies reporting on quantitative health outcomes for paramedics working in Australia or New Zealand. The review was conducted using the JBI methodology for prevalence studies and uses a narrative synthesis approach to reporting. Results There were k = 20 studies that met inclusion criteria, and most used Australian samples. Results indicated between 57.3–66.5% of paramedics studied were classified as overweight or obese, while up to 80% reported poor sleep, and 55.6% reported fatigue. Incidence rates per 100,000 full-time equivalent (FTE) included 26.62 for completed suicide, 5.46 for drug-caused death, and 9.3 for workplace fatalities. The most recent incidence per 1,000 FTE for injury compensation claims was 141.4. Conclusions Australian and New Zealand paramedics demonstrate poor health according to several metrics. Our sample demonstrated considerably worse health than the general population or similar occupations. There is a minimal amount of trend data available; therefore, it was difficult to ascertain if rates are changing. The range of health outcomes studied was limited, and correlations between different health outcomes were rarely considered by authors. Data relating to specific rates for gender and sexuality, location of work, and First Nations status or ethnicity was often not available. SYSTEMATIC REVIEW REGISTRATION NUMBER (PROSPERO) CRD42021232196
Dynamical Change of Cell Mechanical Properties Accompanying and Modulating Metastasis Process

The dynamical change of cellular mechanical properties plays an important role in cell metastasis process, while how the cancer cells modulate their mechanical properties during metastasis are still not fully understood. In this report, the cellular detaching and seeding processes, two vital steps of cell metastasis, were simulated in vitro using a self-developed protocol and characterized by the dynamical mechanical properties using AFM. The measured results show that cells decrease their stiffness and increase their surface adhesion force as they are detaching from substrate, while cells present an opposite change in mechanical properties as they seeding. Additionally, the effect of anti-cancer drug (docetaxel) on the detaching and attaching process of cancer cells (PC-3) was also investigated from the aspect of mechanical properties. The results shows that the docetaxel can increase stiffness, decrease surface adhesion force of PC-3 cell, and slow down the change speed of these mechanical properties during PC-3 cell detaching and seeding process. These discoveries demonstrated that a dynamical change of cell mechanical properties is required for cancer cell metastasis, which provide a new drug development strategy for cancer treatment.
Measuring Six Facets of Curiosity in Germany and the UK: A German-Language Adaptation of the 5DCR and Its Comparability with the English-Language Source Version

Abstract The five-dimensional curiosity-scale revised (5DCR) by Kashdan et al. (2020) is the most comprehensive curiosity inventory available to date. 5DCR measures six facets of curiosity with four items each. Here, we present a German-language adaptation of the 5DCR and comprehensively validate this adaptation in a diverse sample of adults from Germany (N = 486). Moreover, we provide new evidence on the original English-language 5DCR in a parallel sample from the UK (N = 483). In both countries, we investigate the six facets' reliability, factorial validity, and convergent and discriminant validity with a large set of individual-differences constructs. In addition, we analyze the measurement invariance of the curiosity facets across the UK and Germany and across socio-demographic subgroups defined by age, sex, and education. Findings demonstrate that the new German-language adaptation of 5DCR and its English-language source version show psychometric properties similar to the original studies by Kashdan et al. (2020) in the United States. All six curiosity facets reach at least partial scalar invariance across cultures, sex, education, and mostly also across age groups. The findings support the six-faceted theory of curiosity and show that 5DCR allows for a valid assessment of curiosity across cultures.
Multiloop traction method during endoscopic hemostasis for post-sphincterotomy bleeding of the peridiverticular papilla

Periampullary diverticulum (PAD) is considered a risk factor for difficult biliary cannulation during endoscopic retrograde cholangiopancreatography (ERCP) because it makes the papilla unidentifiable [1]. Applying a traction force near the papilla has been reported to be effective in PAD biliary interventions [2]. We report a successful case of endoscopic hemostasis in post-sphincterotomy bleeding, using the multiloop traction method (M-loop method), in a patient with PAD [3] (▶Video 1). A 79-year-old man, taking direct oral anticoagulants (DOACs) for atrial fibrillation, presented with symptomatic common bile duct stones. His DOAC medication was temporarily discontinued on the day of the procedure. A successful endoscopic sphincterotomy (ES), with complete stone removal, was achieved. Post-ES bleeding was noted 4 days after the procedure (▶Fig. 1). Achieving endoscopic hemostasis was challenging because the bleeding source was covered with duodenal mucosal folds. The M-loop method was used to locate and expose the bleeding source (▶Fig. 2). A SureClip (Micro-Tech, Nanjing, China), attached to a multiple-loop-thread, was deployed to cover the mucosal fold. Additional clips were hooked onto the free loop and attached to the oral side of the duodenal wall. A traction force was generated to pull the duodenal mucosa covering the papilla upward. The bleeding point was sufficiently exposed (▶Fig. 3, ▶Fig. 4). A pancreatic stent was placed to avoid post-ERCP pancreatitis. Successful hemostasis was achieved with endoscopic coagulation, using a Coagrasper (Olympus, Tokyo, Japan) (▶Fig. 5). The threads used in the M-loopmethod were cut after hemostasis. Rebleeding was not observed after restarting DOAC therapy. PAD has been reported to be a risk factor for post-ES bleeding [4]. Hemostasis for post-ES bleeding may be challenging in patients with PAD due to the deviation of the papilla’s position. Traction, using the M-loop method, provides adequate exposure of the papilla, facilitating hemostasis in patients with post-ES bleeding.
A Semi-Automated Workflow for Brain Slice Histology Alignment, Registration, and Cell Quantification (SHARCQ)

Visual Abstract Tools for refined cell-specific targeting have significantly contributed to understanding the characteristics and dynamics of distinct cellular populations by brain region. While advanced cell-labeling methods have accelerated the field of neuroscience, specifically in brain mapping, there remains a need to quantify and analyze the data. Here, by modifying a toolkit that localizes electrodes to brain regions (SHARP-Track; Slice Histology Alignment, Registration, and Probe-Track analysis), we introduce a post-imaging analysis tool to map histological images to established mouse brain atlases called SHARCQ (Slice Histology Alignment, Registration, and Cell Quantification). The program requires MATLAB, histological images, and either a manual or automatic cell count of the unprocessed images. SHARCQ simplifies the post-imaging analysis pipeline with a step-by-step GUI. We demonstrate that SHARCQ can be applied for a variety of mouse brain images, regardless of histology technique. In addition, SHARCQ rectifies discrepancies in mouse brain region borders between atlases by allowing the user to select between the Allen Brain Atlas or the digitized and modified Franklin–Paxinos Atlas for quantifying cell counts by region. SHARCQ produces quantitative and qualitative data, including counts of brain-wide region populations and a 3D model of registered cells within the atlas space. In summary, SHARCQ was designed as a neuroscience post-imaging analysis tool for cell-to-brain registration and quantification with a simple, accessible interface. All code is open-source and available for download (https://github.com/wildrootlab/SHARCQ).