Miracle-dz/UniFAR-FTUs huggingface dataset:
Cooperation of p300 and iASPP in apoptosis and tumour suppression

Mutation or functional inactivation of tumour suppressors represents a key event in the transformation of cells and contributes to the development of cancer. P300 and CBP constitute two histone acetyltransferases with tumour suppressor functions that are frequently mutated or functional inactivated in cancer. Accordingly, after chemotherapy-induced DNA damage, for example, p300 and CBP can co-activate the key tumour suppressor proteins p53 and TAp73, thereby contributing to tumour cell apoptosis. Here we investigated the impact of the co-factor protein iASPP on p300 and TAp73 function, after treatment of tumourigenic cell lines with the chemotherapeutic drug cisplatin. iASPP belongs to the ASPP-family, another class of co-factors that contribute to p53-family-mediated apoptosis induction. Direct interaction of iASPP with p300 and TAp73 has been revealed before; functional consequences of these interactions remain elusive though and are therefore subject of our analyses. By investigating the consequences of stable iASPP knockdown in tumourigenic cell lines, we found that direct interaction of iASPP and p300 in cisplatin-treated cells led to enhanced protein stability of p300 and TAp73. Correspondingly, iASPP depletion resulted in decreased protein amounts of p300, reduced induction of pro-apoptotic p73 target genes and impaired apoptosis. BRMS1 represents a recently discovered E3 ubiquitin ligase for p300. Hence, we observed that BRMS1 depletion rescued the degradation of p300 and CBP in cisplatin-treated, iASPP-depleted cells. Therefore, we hypothesize that BRMS1 inhibition constitutes the molecular mechanism underlying the increased protein stability of p300 in the presence of iASPP. Furthermore, we discovered, that malignant melanoma are characterized by down-regulated iASPP expression level. Follow-up studies on melanoma cell lines revealed that low iASPP expression correlated with decreased protein levels of p300 in cisplatin-treated cells. BRMS1 knockdown in some of these cell lines could up-regulate p300 protein level, suggesting that down-regulation of iASPP expression leads to functional inactivation of p300 in melanoma, by allowing BRMS1 activity on p300. Treatment of the cells with the MKP-1 inhibitor BCI, could re-establish p300 level and induce p300-dependent apoptosis by yet unknown mechanisms. Summing up, iASPP represents a regulator for p300 function. It contributes to p300-dependent apoptosis induction by enhancing its protein stability after DNA damage. Re-establishment of p300 levels in low iASPP-expressing melanoma might represent a novel strategy to overcome chemoresistance.
Automated Long-Term Total Collection Versus Indicator Method to Estimate Duodenal Digesta Flow in Cattle

Four steers, fitted with re-entrant duodenal cannulas, were used to compare digesta flow as measured by automated total collection (ATC) with flow estimations based on chromium oxide (Cr z O s ) and lignin concentrations of representative samples of 24-h collection of digesta. In two successive periods, each steer was fed about 4 kg/d of either an all-forage or an 80% sorghum grain diet. Duodenal digesta samples were automatically taken and pooled every 2 h, during the 3to 6-d total collections. For animals adapted to the collection procedures, there was no consistent evidence of flow inhibition in the first 24 h of a long-term total collection. Flow rates of duodenal digesta and dry matter (DM), estimated by Cr20 s and lignin were 15% greater and more variable than those measured by ATC. Digesta flow rates measured by ATC averaged 65 iiters/d in steers fed the forage diet and 42 liters/d in the same steers receiving the grain diet. Apparent ruminal DM disappearance, calculated from direct measurements by ATC was 44% for the grain diet and 37% for the forage diet. Ruminal DM disappearance, based on Cr 20 s and lignin, was about 25% lower than the ATC values. The data indicate that the use of total collection procedures should include replication over days. Duodenal flow rates suggested a 24-h cyclic pattern of digesta flow for the grain diet, with a peak about midnight followed by a period of low flow before the onset of the light hours. Chromium oxide showed a similar flow pattern, but flow rate decreased more rapidly following the midafternoon peak. With the forage diet, 24-h patterns were only observed for Cr~O s and lignin concentrations in duodenal digesta, which appeared different from the Cr: O s concentration pattern with the grain diet. Diurnal variation of digesta flow was greater than day-to-day variation. Because of the diurnal variation, spot-sampling techniques must be considered very carefully, since inadequate sampling could easily misrepresent total collection samples and flow estimates. The data also suggest caution in the use of lignin as a marker in grain diets, and of Cr z O s as a marker in forage diets for partitioning digestion in the duodenum of ruminants. (
Tm-Link: An Internationally Linked Trade Mark Database

This article describes a new database—TM‐Link—that contains 12 million trademark applications and registrations across six jurisdictions. A feature of the database is the identification of trademark equivalents (or families) within and across national trademark offices. Equivalent trademarks are two, or more, insignias for the same product applied for by the same company. Unlike patents, the incentive to file for global priority is comparatively weak since legal priority for trademarks is territorial. To identify the number of true trademark equivalents we therefore create synthetic links using a neural network‐based machine learning algorithm.
When Elephants Make Peace: The Impact of the China-U.S. Trade Agreement on Developing Countries

Should the China-U.S. trade agreement prompt relief because it averts a damaging trade war or concern because selective preferential access for the United States to China's markets breaks multilateral rules against discrimination? The answer depends on how China implements the agreement. Simulations from a computable general equilibrium model suggest that the United States and China would be better off under this"managed trade"agreement than if the trade war had escalated. However, compared with the policy status quo, the deal will make everyone worse off except the United States and its input-supplying neighbor, Mexico. Real incomes in the rest of world would decline by 0.16 percent and in China by 0.38 percent because of trade diversion. China can reverse those losses if, instead of granting the United States privileged entry, it opens its market for all trading partners. Global income would be 0.6 percent higher than under the managed trade scenario, and China's income would be nearly 0.5 percent higher. By creating a stronger incentive for China to open its markets to all, an exercise in bilateral mercantilism has the potential to become an instrument for multilateral liberalization.
THE EFFECT OF MANAGEMENT FUNCTION ON THE MENTAL HEALTH STATUS OF MILENNIALS HEALTH WORKERS IN COMMUNITY HEALTH CENTERS IN GORONTALO CITY

Mental health is someone who is free from tension and anxiety. Mental health in general is behavior that can be accepted by the community, his lifestyle is in accordance with the norms prevailing in the community so that there is a satisfying relationship between individuals in his social life, the individual's psychological life is relatively stable, does not harbor many internal conflicts, his mood is calm and even his body is always in good health. Whereas someone with an unhealthy person is someone who is mentally disturbed both because of inner conflict, so that his soul is unstable, does not have attention to the environment, feels always right, separated and or avoided by the environment, feels scared, and is also restless. This study aims to determine the effect of management functions on the mental health status of millennial health workers in Community Health Centers throughout Gorontalo City. This research uses quantitative research methods with a survey approach. The research sample of 116 millennial health workers spread in 10 Community Health Centers throughout Gorontalo City. Data collection uses a questionnaire containing a mental health questionnaire and management functions. Data were analyzed by simple linear regression analysis through the SPSS program for windows. The results showed that the management function had no effect on mental health status with a t-value of 0.730> 0.05. It is expected that in future studies need to consider other factors that affect mental health.
The transducer-like proteins of Campylobacter jejuni

The transducer-like proteins of Campylobacter jejuni Randeep Sandhu, BSc. (Hons) Campylobacter jejuni is the leading cause of gastrointestinal disease in the developed world. Chemotactic motility is a pre-requisite for intestinal colonisation by C. jejuni. In silico analysis of the C. jejuni NCTC 11168 genome identified homologues of 10 chemotaxis receptor and two aerotaxis genes. Six of the ten putative Transducer-like proteins (Tlp 1, 2, 3, 4, 7 and 10) resemble chemoreceptors of Escherichia coli. The aim of this project is to characterise the C. jejuni Tlp1-4 chemoreceptors. The genes encoding the Tlps were inactivated using an insertional inactivation strategy. Isogenic mutants were made in tlp1, tlp2 and tlp4; a final mutant in tlp3 could not be obtained. A tlp1 complement was also constructed in this work. The tlp1 mutant showed reduced chicken colonisation ability when tested by our collaborators. Chemotactic phenotypes of the tlp mutants were determined in the swarm assay; the tlp mutants appeared defective for chemotaxis when compared with the wild-type and non-motile flaAB mutant. The Capillary assay and Hard-Agar Plug (HAP) assay were developed as methods to ascertain the ligand specificities of the Tlp chemoreceptors under study. Unfortunately, the Capillary assay proved to be insufficiently reproducible for effective use with C. jejuni. The HAP procedure was optimised using a C. jejuni wild-type motile variant. Positive chemoattractant responses were observed in NCTC 11168 for the first time towards a range of chemicals. Data derived from the modified HAP assay indicated that Tlp1 may be the receptor for serine. Chemotactic responses could not be detected in the tlp2 and tlp4 mutants in the HAP assay. The signalling domain of Tlp1 was purified using a polyhistidine tag and used to produce a polyclonal antibody. The Tlp1 primary antibody and immunofluorescence labeling has shown for the first time that the Tlps cluster at the cell poles in C. jejuni. The transducer-like proteins of Campylobacter jejuni. Randeep Sandhu
The Relationship between Interpersonal Relationships and Suicidal Ideation of Male Rehabilitation Protection Recipients with Alcohol Problems: The Mediating Effect of Depression

The purpose of this study was to investigate the role of depression in the relationship between interpersonal relationships and suicide ideation among male rehabilitation protection recipients with alcohol problems. The participants of this study were 184 male rehabilitation protection recipients with alcohol problems. The tools used were AUDIT-K, Interpersonal Needs Questionnaire-Revised, Scale for Suicide Ideation-Beck, and Beck Depression Inventory-K. The results were that depression played a partial mediator role in interpersonal relationships and suicidal ideation.
Post-harvest Bacterial Quality of Lethrinus lentjan (Lacepede, 1802)

Food safety and microbiological quality particularly in high moisture foods like seafood have gained significant attention among consumers. Compromise with regard to quality standards for the retail trade of fish and fish products is decreasing the quality of fish. Present study was conducted to detect the bacterial progression fish from harbour to retail shop and market. Lethrinus lentjan (white snapper) was collected from three different strategic locations, viz., harbour, retail shop and market on the same day and quantitative and qualitative bacteriological analyses were carried out. Samples collected from market exhibited 12.61% more mesophilic count than that of harbour samples and 9.5% more than retail samples. The increasing trend in bacterial counts was noticed in psychrophiles, enterobacteriaceae, faecal streptococci, hydrogen sulphide producers, Brochothrix thermosphacta and histamine producing bacteria. Pathogens like Salmonella, Vibrio cholerae, V. parahaemolyticus and Lethrinus lentjan, microbial quality, harbour, retail market Listeria monocytogenes were absent in the analysed samples.
Time accelaration of offshore EPC project using FMEA, FTA, CPM and crashing method at PT XYZ

The pipe instalation of offshore EPC project has high risk of cost overruns due to the uncertainty of weather, human errors, pipe fabriction process, etc. All of those risks will cause the project delay. The optimiziation of risk based project scheduling can reduce the risks. Risk management can be optimized with combining some methods, such as Failure Mode and Effect Analysis (FMEA), Fault Tree Analysis (FTA), Critical Path Method (CPM) and Crashing Project method. The combination of these methods are used to do the data analysis in order to reduce the project total duration. Based on analysis using FMEA, FTA, CPM and crashing project method, the total of project duration can be reduced from 499 days to 489 days with Rp. 6.822. 472,- as the additional cost of workers.
Detection and Validation of Agronomic and Seed Quality Quantitative Trait Loci in Soybean

Soybean seed quality and agronomic traits are important commercially. Agronomic traits such as yield, plant height, lodging, and adapted maturity have been the primary focus of breeders for many years. Seed quality traits are also important as they affect the market price of soybean. Higher protein soybean historically is valued more per unit. It is the goal of plant breeders therefore to simultaneously improve seed quality and agronomic traits. Seed quality and agronomic traits are quantitative traits whose inheritance is governed by many genes, and whose expression is subject to environmental variation. Furthermore, negative correlations between yield and protein, and protein and oil make it even more difficult to select for these traits. Molecular breeding tools such as quantitative trait loci (QTL) can provide breeders with a more direct method of selection for traits at the molecular level. QTL can however be misleading as they are subject to type I and type I1 errors. QTL validation studies are essential to marker assisted programs as they negate the need for individual breeders to validate every QTL of interest. The purpose of this study was to validate previously reported seed quality and agronomic trait QTL in an independent population derived fiom an Essex x Williams 82 cross. We were able to validate QTL for all traits and detected novel QTL that may be useful to breeders.
A New Approach to Identifying Crash Hotspot Intersections (CHIs) Using Spatial Weights Matrices

In this paper we develop a new approach to directly detect crash hotspot intersections (CHIs) using two customized spatial weights matrices, which are the inverse network distance-band spatial weights matrix of intersections (INDSWMI) and the k-nearest distance-band spatial weights matrix between crash and intersection (KDSWMCI). This new approach has three major steps. The first step is to build the INDSWMI by forming the road network, extracting the intersections from road junctions, and constructing the INDSWMI with road network constraints. The second step is to build the KDSWMCI by obtaining the adjacency crashes for each intersection. The third step is to perform intersection hotspot analysis (IHA) by using the Getis–Ord Gi* statistic with the INDSWMI and KDSWMCI to identify CHIs and test the Intersection Prediction Accuracy Index (IPAI). This approach is validated by comparison of the IPAI obtained using open street map (OSM) roads and intersection-related crashes (2008–2017) from Spencer city, Iowa, USA. The findings of the comparison show that higher prediction accuracy is achieved by using the proposed approach in identifying CHIs.
Anti-alcohol campaigns effects depending on target drinking levels and source perception

Alcohol consumption represents a major problem for public health. As a consequence, there is a growing interest in understanding which communication strategies have the ability to increase the persuasive power of anti-alcohol campaigns. Many designers of health campaigns use both actors and real people interchangeably to deliver their messages. Nevertheless, little is known regarding which of these two types of message sources are more persuasive. Aim & Methodology. In this study, we used an experimental design to explore the effect of recipients’ perceptions of the message source (presented as either real people or actors) on their drinking intentions. Results. We observed a significant difference in the drinking intentions as an effect of our manipulation, depending on previous recipients’ drinking levels. Heavy consumers were more readily persuaded by the real source message. In contrast, light consumers showed less drinking intentions after receiving the message from the fictional source than from the real one. Conclusions. This indicates that in the context of anti-alcohol campaigns, the same strategy could have both positive and negative effects simultaneously on different target groups. Theoretical and practical implications of our findings are discussed.
Farmers’ Intentions to Lease Forestland: Evidence from Rural China

In the last decade, despite considerable research developed for the forestland leasing market, little has been published in terms of econometric results on determinants of intentions and behaviors of Chinese farmers. With respect to leasing forestland, this study uses a Bayesian logit model to examine the factors that influence farmers’ intentions, using household data collected in one county in 2017. The results show that farmers’ past experience of leasing forestlands have significant impacts on their leasing intentions. Once farmers participated in leasing in or leasing out forestland in the last five years, it was shown that they will have stronger intentions of doing so in the future. Farmers will neither lease in or out forestland if the leasing profits are less than the profits originated from forestland management. As such, household head age, household population, proportion of income from nonfarm sources to total income, and security of rights to forestland use are significant factors in influencing farmers’ decisions on leasing in forestland. On the other hand, household head age and educational level, proportion of income from nonfarm sources to total income, and importance of forestland in terms of inheritance are significant factors in influencing farmers’ decisions on leasing it out. Results imply that institutional and market factors, which have impacts on transaction costs, are important for farmers in making decisions on forestland leases. Policy implications to reduce institutional intervention are discussed.
Natural antioxidants in cancer prevention

It is well known that oxidative stress is an inevitable event in aerobic life. When our cells use oxygen to create energy, a variety of reactive oxygen (ROS) and nitrogen species (RNS) are generated. These species could attack DNA directly and form mutagenic lesions afterwards. According to the oxidative stress hypothesis of aging, the oxidative damage to critical molecules accumulates over the life period and could ultimately impair the body’s function. Moreover, severe oxidative stress causes mutations of tumor suppressor genes, known as one of the initial events in carcinogenesis. Furthermore, it could also play a crucial role in the promotion of the multi-step carcinogenesis. On the other hand, the human body possesses a number of mechanisms that counteract oxidative stress by producing antioxidants in situ, or externally supplied them through foods and/or supplements. Indeed, a considerable amount of laboratory evidence from chemical, cell culture, and animal studies indicates that antioxidants may slow down or possibly prevent the cancer development. Yet, the information from recent cohort, case-control and/or ecological studies is less clear. Therefore, the objectives of this review are to compile a compendium of studies, and to identify effective and promising natural antioxidant interventions.
Speaker Feature Modeling Utilizing Constrained Maximum Likelihood Linear Regression and Gaussian Mixture Models

This paper describes a speaker recognition system based on feature extraction utilizing the constrained maximum likelihood linear regression (CMLLR) speaker adaptation, while using Gaussian mixture models (GMM) to model the speaker and background models. For the input acoustic signals, the cepstral features are derived to highlight the differences between test and training utterances. The CLSU dataset is used to test the efficiency and performance of the proposed CMLLR, Support Vector Machine, and GMM methods for modeling the speaker’s voice by characterizing the speaker features.
Fracture performance of a PCM-Rich concrete pavement under thermal stresses

ABSTRACT Curling stresses in rigid pavements are the result of temperature gradient through the thickness of the concrete slab. These stresses have a periodic nature, and the induced cyclic flexural curling stresses lead to the cracking of the concrete slab. In a previous research by the authors, a phase change material (PCM)-rich concrete overlay was proposed as an effective method to control this failure mechanism. A PCM-rich concrete overlay is a concrete surface layer that contains a relatively high percentage of PCM. In the previous study, a phenomenological approach was employed, and it was demonstrated that the incorporation of PCM improves the curling fatigue performance of the concrete slab. Using fracture mechanics, this paper builds upon the findings of the previous study, and it serves as a mechanistic verification of the conclusions of the previous work. A modified Paris’ Law was adopted to calculate the cumulative fatigue-fracture damage of the concrete slab due to cyclic thermally-induced curling stresses. The findings of this research demonstrated that the PCM-modified concrete slab undergoes a curling-induced fracture process that is less severe than the slab without the PCM. The impact of this reduction was quantified in terms of an improved fatigue-fracture performance of the pavement.