Miracle-dz/UniFAR-FTUs huggingface dataset:
Frontal Asymmetry Analysis of Theta Wave in the Audio Emotional Experiment Revealed by Event-related Spectral Perturbation

Hemispheric asymmetry in prefrontal activation have been proposed in two decades ago, as measured by electroencephalographic (EEG) power in the theta band (4-8Hz), is related to reactivity to affectively pleasure audio stimuli. In this study, we designed an emotional audio stimulus experiment in order to verify frontal EEG asymmetry by analyzing ERSP results. Thirty healthy college students volunteered the stimulus experiment with the standard IADS affective sounds. These affective sound clips are classified in three emotion states, happy, neutral and fear. ERSP image results revealed that there are the stronger responses of high arousal (fear and happy) in the left pre-frontal lobe, while the stronger responses of low arousal (neutral) in the right pre-frontal lobe. However, the high pleasure emotions (happy) can elicit greater relative right EEG activity, while the low and middle pleasure emotions (fear and neutral) can elicit the greater relative left EEG activity. Additionally, the most response differences of theta band have been found out in the medial frontal lobe, which is proved as the frontal midline theta.
Direct and indirect effects of near-future pCO2 levels on zooplankton dynamics

Ocean acidification has direct physiological effects on organisms, for example by dissolving the calcium carbonate structures of calcifying species. However, non-calcifiers may also be affected by changes in seawater chemistry. To disentangle the direct and indirect effects of ocean acidification on zooplankton growth, we undertook a study with two model organisms. Specifically, we investigated the individual effects of short-term exposure to high and low seawater pCO2, and different phytoplankton qualities as a result of different CO2 incubations on the growth of a heterotrophic dinoflagellate (Oxyrrhis marina) and a copepod species (Acartia tonsa). It was observed previously that higher CO2 concentrations can decrease phytoplankton food quality in terms of carbon:nutrient ratios. We therefore expected both seawater pCO2 (pH) and phytoplankton quality to result in decreased zooplankton growth. Although we expected lowest growth rates for all zooplankton under high seawater pCO2 and low algal quality, we found that direct pH effects on consumers seem to be of lesser importance than the associated decrease in algal quality. The decrease in the quality of primary producers under high pCO2 conditions negatively affected zooplankton growth, which may lead to lower availability of food for the next trophic level and thus potentially affect the recruitment of higher trophic levels.
Multi-drug resistant Klebsiella pneumoniae causing urinary tract infections in children in Pakistan

Multi-drug resistant (MDR) Klebsiella pneumoniae has been associated with different types of infections and the most important aspect is the emergence of MDR strains particularly in hospitalized children. They have the proficiency to cause bacteremia, septicemia and urinary tract infections (UTI). The objective of this study was to determine the susceptibility of MDR K. pneumoniae causing UTI in children. One thousand and fifteen (1015) urine samples were collected aseptically. Specimens were cultured on blood agar, MacConkey agar and cysteine lactose electrolyte deficient (CLED) agar. Antimicrobial susceptibility was determined using Kirby-Bauer disc diffusion method as per CLSI 2011 guidelines. Of the 1015 urine specimens, 230 (22.6%) were positive for bacterial growth. Out of these positive cultures predominantly Gram-negative rods (90%) were isolated and major pathogens were K. pneumoniae (40%) and Escherichia coli (33%). Antimicrobial susceptibility pattern of K. pneumoniae showed that more than 70% of these pathogens were resistant to cephalosporins, 69% to ciprofloxacin and amoxicillin-clavulanic acid and 63% to norfloxacin and nalidixic acid while most effective drugs were pipracillin-tazobactam and meropenem. This study concludes that MDR-K. pneumoniae is a great threat particularly in children in our locality. Key words: Multi-drug resistant (MDR), Klebsiella pneumoniae, children, antimicrobial resistance (AMR).
Spectroscopic Evidence for an Additional Symmetry Breaking in the Nematic State of FeSe Superconductor

The iron-based superconductor FeSe has attracted much recent attention because of its simple crystal structure, distinct electronic structure and rich physics exhibited by itself and its derivatives. Determination of its intrinsic electronic structure is crucial to understand its physical properties and superconductivity mechanism. Both theoretical and experimental studies so far have provided a picture that FeSe consists of one hole-like Fermi surface around the Brillouin zone center in its nematic state. Here we report direct observation of two hole-like Fermi surface sheets around the Brillouin zone center, and the splitting of the associated bands, in the nematic state of FeSe by taking high resolution laser-based angle-resolved photoemission measurements. These results indicate that, in addition to nematic order and spin-orbit coupling, there is an additional order in FeSe that breaks either inversion or time reversal symmetries. The new Fermi surface topology asks for reexamination of the existing theoretical and experimental understanding of FeSe and stimulates further efforts to identify the origin of the hidden order in its nematic state.
The effect of split-root salinity stress on tomato leaf expansion, fruit yield and quality

Summary A crop-scale experiment between March and October 2000 investigated the effects of split-root salinity stress and rooting volume on the expansion of leaves, fruit yield and quality in two standard round fruited cultivars of tomato (Lycopersicon esculentumMill) Solairo and Espero, grown in rockwool. To implement the treatments, a split-root method was utilized whereby different levels of salinity (expressed as EC), adjusted by the addition of NaCl, was administered to two halves of a root system. The split-root high EC (2.8/8.0 dS m–1) treatment enhanced fruit quality by increasing the concentrations of sugar, acid, total soluble solids and reducing the incidence of visual defects such as uneven ripening and gold-spot. Split-root high EC did however reduce leaf area that corresponded with low K concentration in the leaf tissue. The split-root method utilized doubled the rooting volume compared with the standard single rockwool slab, but this extra rooting capacity had no detectable effect on leaf expansion, yield or fruit quality. Although there was little impact on yield for ten weeks after the imposition of the high EC split-root treatment, mean reductions in weekly yield occurred thereafter and were of the order of 9 and 13% for ‘Solairo’ and ‘Espero’ respectively through to the end of the experiment. The fall in yield was due, in part, to a decrease in fruit size. Whilst water uptake was reduced it was unclear how the effects on leaf growth and fruit yield were mediated in the shoot. Possible processes that coordinate responses to root-zone salinity stress are discussed. The potential use of the split-root system in commercial production is considered.
HTS-IBIS: fast and accurate inference of binding site motifs from HT-SELEX data

Summary Recent technological advancements enable measuring the binding of a transcription factor to thousands of DNA sequences, in order to infer its binding preferences. High-throughput-SELEX measures protein-DNA binding by deep sequencing over several cycles of enrichment. We devised a new algorithm called HTS-IBIS for the inference task. HTS-IBIS corrects for technological biases, selects the cycle and k, and builds a motif starting from a consensus k-mer in that cycle. In large scale tests, HTS-IBIS outperformed the extant automatic algorithm for the motif finding task on both in vitro and in vivo binding prediction. Availability HTS-IBIS is available on acgt.cs. tau.ac. il/HTS-IBIS. Contact rshamir@tau.ac. il
DNA Methylation and Immune Cell Markers Demonstrate Evidence of Accelerated Aging in Patients with Chronic HBV or HCV, with or without HIV Co-Infection.

BACKGROUND Several chronic diseases have been shown to accelerate biological aging. We investigated age acceleration and the association between peripheral blood DNAm and immune cell markers in patients chronically infected with the hepatitis B virus (HBV) or the hepatitis C virus (HCV) with and without human immunodeficiency virus (HIV) co-infection. METHODS Age acceleration was measured as the difference between epigenetic age (Horvath clock) and chronological age. The immune marker model of age acceleration was developed using Elastic Net regression to select both the immune markers and their associated weights in the final linear model. RESULTS Patients with chronic HBV (n=51) had a significantly higher median epigenetic age compared to chronological age (age accelerated) (p < 0.001). In patients with chronic HCV infection (n=63), age acceleration was associated with liver fibrosis as assessed by histology (p < 0.05), or presence of HIV co-infection (p < 0.05), but not HCV mono-infection. Age acceleration defined by immune markers was concordant with age acceleration by DNA methylation (correlation coefficient=0.59 in HBV; p=0.0025). One-year treatment of HBV patients with nucleoside therapy was associated with a modest reduction in age acceleration as measured using the immune marker model (-0.65 years, p=0.018). CONCLUSION Our findings suggest that patients with chronic viral hepatitis have accelerated epigenetic aging and that immune markers defines biological age and has the potential to assess the effects of therapeutic intervention on age acceleration.
Trajectories of sexual well-being among couples in the transition to parenthood.

Sexual well-being is an important contributor to romantic relationship quality, health, and quality of life, yet couples face significant disruptions to their sexuality during the transition to parenthood. While there is evidence of variability in the sexual well-being of new parents, distinct classes of dyadic trajectories have not been established. Sexual frequency, desire, satisfaction, and distress are each unique components of sexual well-being and may follow different patterns of change within couples. We sought to establish classes of trajectories of four aspects of sexual well-being for couples in the transition to parenthood as well as the associations among identified classes. Couples (N = 203) expecting their first child completed online standardized measures of sexual well-being at 20 and 32 weeks in pregnancy and at 3, 6, 9, and 12 months postpartum. Dyadic latent class growth analyses were conducted to identify classes of trajectories for each facet of sexual well-being, and dual trajectory analyses examined the probability of group membership across classes. Couples' sexual well-being over time was heterogeneous. Sexual frequency had two classes: high (33%) and low (67%); sexual desire had three classes: moderate (36%), high (25%), and discrepant (39%); sexual satisfaction had two classes: high (64%) and low (36%); and sexual distress had two classes: low (76%) and discrepant (24%). Overlap in classes of sexual well-being was variable such that high and low or discrepant (between partners) classes did not always co-occur. Findings provide more nuanced information about new parents' postpartum sexual health, which may facilitate early assessment and intervention. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
A 3 year post‐intervention follow‐up on mortality in advanced heart failure (EVITA vitamin D supplementation trial)

Vitamin D supplementation is widely used in the clinical setting, but its effects on mortality and cardiovascular outcomes in patients with heart failure are unclear. This paper reports outcome data that were collected during follow‐up of 3 years after closure of the EVITA trial (a 3 year randomized, placebo‐controlled, intervention study with 4000 IU vitamin D daily in patients with advanced heart failure), to capture potential latency effects of vitamin D supplementation on clinical outcomes.
Epidemiology of early onset dementia and its clinical presentations in the province of Modena, Italy

Patients with early onset dementia (EOD), defined as dementia with symptom onset at age <65, frequently present with atypical syndromes. However, the epidemiology of different EOD presentations, including variants of Alzheimer's disease (AD) and frontotemporal dementia (FTD), has never been investigated all together in a population‐based study. Epidemiologic data of all‐cause EOD are also scarce.
Plasmonic and Electrostatic Interactions Enable Uniformly Enhanced Liquid Bacterial Surface-Enhanced Raman Scattering (SERS)

Surface-enhanced Raman spectroscopy (SERS) is a promising cellular identification and drug susceptibility testing platform, provided it can be performed in a controlled liquid environment that maintains cell viability. We investigate bacterial liquid-SERS, studying plasmonic and electrostatic interactions between gold nanorods and bacteria that enable uniformly enhanced SERS. We synthesize five nanorod sizes with longitudinal plasmon resonances ranging from 670 to 860 nm and characterize SERS signatures of Gram-negative Escherichia coli and Serratia marcescens and Gram-positive Staphylococcus aureus and Staphylococcus epidermidis bacteria in water. Varying the concentration of bacteria and nanorods, we achieve large-area SERS enhancement that is independent of nanorod resonance and bacteria type; however, bacteria with higher surface charge density exhibit significantly higher SERS signal. Using cryo-electron microscopy and zeta potential measurements, we show that the higher signal results from attraction between positively charged nanorods and negatively charged bacteria. Our robust liquid-SERS measurements provide a foundation for bacterial identification and drug testing in biological fluids.
Potential of discarded sardine scales (Sardina pilchardus) as chitosan sources

ABSTRACT The random discharge of marine fish waste into the coast generates environmental pollution. However, a better valorization of these by-products leads to the extraction of sustainable biomolecules. Chitosan is a natural biopolymer that can be produced from various marine by-products, in particular the crustacean shells, crabs, and fish scales. The aim of this current study is the extraction of chitin and characterization of chitosan obtained after a deacetylation reaction from sardine scales (S. pilchardus) as a new marine source. The β form of chitin extracted undergoes deacetylation in 40% NaOH at 121°C for 20 min. The chemical structure of obtained chitosan was characterized based on Fourier transforms infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Scanning electron microscope (SEM), and Energy-dispersive X-ray spectroscopy (EDS). The physicochemical properties of obtained chitosan such as the ash, moisture, nitrogen, solubility, molecular weight, fat, and water-binding capacity were also determined. According to the results of FTIR and XRD analysis, the degree of deacetylation (DDA), and the crystalline index (CrI) value of obtained chitosan is respectively about 87% and 95%. The SEM and EDS analysis revealed respectively fibrillar and pleated morphology with the presence of three major elements characterizing the chitosan, which are C, O, and N. The physicochemical analysis showed that the rate of ash, moisture, and nitrogen in obtained chitosan were respectively about 0.10, 0.34, and 7%. The solubility, molecular weight, fat, and water-binding capacity of produced chitosan were found to be 93%, 5.86 kDa, 310, and 510% respectively. Sardina pilchardus scales could be considered a promising and alternative source of chitin and chitosan, which will be applicable in a large number of fields. Implications: Direct rejection of marine biowaste as fish scales in nature, port, or fish processing plants, is a dramatic problem that is growing day after day. These uncontrollable discharges cause marine pollution and promote bacterial growth, which leads to a degradation of the soil and air quality. Taking into account the objectives of sustainable development, better development of these by-products would make it possible to produce valuable biomaterials that will be applied in various fields and which have benefits for the environment and humans. The central objective of this research is accentuated on the enhancement of Sardina pilchardus scales; by the conversion of chitin into chitosan and the determination of its physicochemical characterization. The obtained chitosan from Sardina pilchardus scales could be applied in the agricultural and food industry.
Transthyretin Amyloidogenesis Inhibitors: From Discovery to Current Developments.

Transthyretin (TTR) is a homo-tetrameric protein in human plasma. The dissociation of the TTR tetramer and misfolding of the TTR monomer result in the formation of amyloid fibrils. Hereditary TTR amyloidosis is characterized by the extracellular deposition of amyloid fibrils containing TTR variants. The development of small molecules that kinetically stabilize the TTR tetramer is one of the effective strategies for the treatment of hereditary TTR amyloidosis. So far, several stabilizers have been discovered. Tafamidis is the only approved stabilizer for treatment of hereditary TTR amyloidosis, although two nucleic acid medicines that inhibit TTR synthesis, inotersen and patisiran, were recently approved for treatment of this disorder. In this perspective, we seek to describe the representative kinetic stabilizers from discovery to development, interweaving the crystallographic study of the complex structures.
Effect of Sub-Stoichiometric Fe(III) Amounts on LCFA Degradation by Methanogenic Communities

Long-chain fatty acids (LCFA) are common contaminants in municipal and industrial wastewater that can be converted anaerobically to methane. A low hydrogen partial pressure is required for LCFA degradation by anaerobic bacteria, requiring the establishment of syntrophic relationships with hydrogenotrophic methanogens. However, high LCFA loads can inhibit methanogens, hindering biodegradation. Because it has been suggested that anaerobic degradation of these compounds may be enhanced by the presence of alternative electron acceptors, such as iron, we investigated the effect of sub-stoichiometric amounts of Fe(III) on oleate (C18:1 LCFA) degradation by suspended and granular methanogenic sludge. Fe(III) accelerated oleate biodegradation and hydrogenotrophic methanogenesis in the assays with suspended sludge, with H2-consuming methanogens coexisting with iron-reducing bacteria. On the other hand, acetoclastic methanogenesis was delayed by Fe(III). These effects were less evident with granular sludge, possibly due to its higher initial methanogenic activity relative to suspended sludge. Enrichments with close-to-stoichiometric amounts of Fe(III) resulted in a microbial community mainly composed of Geobacter, Syntrophomonas, and Methanobacterium genera, with relative abundances of 83–89%, 3–6%, and 0.2–10%, respectively. In these enrichments, oleate was biodegraded to acetate and coupled to iron-reduction and methane production, revealing novel microbial interactions between syntrophic LCFA-degrading bacteria, iron-reducing bacteria, and methanogens.
Alternative splicing at neuroligin site A regulates glycan interaction and synaptogenic activity

Post-transcriptional mechanisms regulating cell surface synaptic organizing complexes that control the properties of connections in brain circuits are poorly understood. Alternative splicing regulates the prototypical synaptic organizing complex, neuroligin-neurexin. In contrast to the well-studied neuroligin splice site B, little is known about splice site A. We discovered that inclusion of the positively charged A1 insert in mouse neuroligin-1 increases its binding to heparan sulphate, a modification on neurexin. The A1 insert increases neurexin recruitment, presynaptic differentiation, and synaptic transmission mediated by neuroligin-1. We propose that the A1 insert could be a target for alleviating the consequences of deleterious NLGN1/3 mutations, supported by assays with the autism-linked neuroligin-1-P89L mutant. An enrichment of neuroligin-1 A1 in GABAergic neuron types suggests a role in synchrony of cortical circuits. Altogether, these data reveal an unusual mode by which neuroligin splicing controls synapse development through protein-glycan interaction and identify it as a potential therapeutic target.
Understanding staff- and system-level contextual factors relevant to trauma-informed care implementation.

Objective: Trauma-informed care (TIC) is increasingly used to address and prevent adverse childhood experiences. However, research on the implementation process of TIC has lagged, limiting its impact and sustainability. The goal of this two-study project was to provide a summary of staff- and system-level contextual factors relevant to TIC implementation. Method: Study 1 was a secondary data analysis of survey data from 760 staff members from human services, health, and education practice settings familiar with TIC. Independent-samples t tests were used to evaluate differences between lower- and higher-implementing organizations on staff- and system-level TIC implementation drivers. These drivers were then coded using the Consolidated Framework for Implementation Research (CFIR). Study 2 was a content analysis of the empirical literature on TIC. Study 2 produced information on the staff- and system-level facilitators of and barriers to TIC implementation, which were then also coded using the CFIR. Results: Study 1 suggested that the attributes of the individuals implementing TIC and the implementation climate of the organization played the most central roles. Study 2 identified available resources and the strength and quality of the evidence underpinning the intervention as important contextual factors for TIC implementation. Conclusion: This study used a well-established framework, the CFIR, to elucidate key contextual factors related to the successful implementation of TIC, with the goal of informing the efforts of researchers, practitioners, and policymakers. (PsycInfo Database Record (c) 2020 APA, all rights reserved).