Miracle-dz/UniFAR-FTUs huggingface dataset:
Cultural relics protecting design of subway construction in Xi'an

Through deep investigation of natural and historical environment,historical layout,large relics in ancient city Xi'an,it introduces the cultural relics protecting design of line net from many aspects,compares the plans in designing process,so as to do well in relics protecting and creating conditions,and achieve the aims of ensuring the safety of relics and not affecting the general situation of subway construction.
Patient Information Items Needed to Guide the Allocation of Scarce Life-Sustaining Resources: A Delphi Study of Multidisciplinary Experts

Abstract Objective: Plans for allocation of scarce life-sustaining resources during the coronavirus disease 2019 (COVID-19) pandemic often include triage teams, but operational details are lacking, including what patient information is needed to make triage decisions. Methods: A Delphi study among Washington state disaster preparedness experts was performed to develop a list of patient information items needed for triage team decision-making during the COVID-19 pandemic. Experts proposed and rated their agreement with candidate information items during asynchronous Delphi rounds. Consensus was defined as ≥80% agreement. Qualitative analysis was used to describe considerations arising in this deliberation. A timed simulation was performed to evaluate feasibility of data collection from the electronic health record. Results: Over 3 asynchronous Delphi rounds, 50 experts reached consensus on 24 patient information items, including patients’ age, severe or end-stage comorbidities, the reason for and timing of admission, measures of acute respiratory failure, and clinical trajectory. Experts weighed complex considerations around how information items could support effective prognostication, consistency, accuracy, minimizing bias, and operationalizability of the triage process. Data collection took a median of 227 seconds (interquartile range = 205, 298) per patient. Conclusions: Experts achieved consensus on patient information items that were necessary and appropriate for informing triage teams during the COVID-19 pandemic.
Diagnosis and follow‐up of glycogen storage disease (GSD) type VI from the largest GSD center in China

Glycogen storage disease (GSD) Type VI is a glycogenolysis disorder caused by variants of PYGL. Knowledge about this disease is limited because only approximately 50 cases have been reported. We investigated the clinical profiles, molecular diagnosis, and treatment outcomes in patients with GSD VI from 2000 to 2021. The main initial clinical features of this cohort include hepatomegaly, short stature, elevated liver transaminases, hypertriglyceridemia, fasting hypoglycemia, and hyperuricemia. After uncooked cornstarch treatment, the stature and biochemical parameters improved significantly (p < 0.05). However, hyperuricemia recurred in most patients during adolescence. Among the 56 GSD VI patients, 54 biallelic variants and two single allelic variants of PYGL were identified, of which 43 were novel. There were two hotspot variants, c.1621‐258_2178‐23del and c.2467C>T p.(Gln823*), mainly in patients from Southwest and South China. c.1621‐258_2178‐23del is a 3.6 kb deletion that results in an out‐of‐frame deletion r. 1621_2177del and an in‐frame deletion r. 1621_2265del. Our data show for the first time that long‐term monitoring of uric acid is recommended for older GSD VI patients. This study also broadens the variant spectrum of PYGL and indicates that there are two hot‐spot variants in China.
Risk factors for short-term re-bleeding in patients with colonic diverticular bleeding: a multicenter retrospective study

Abstract Background and Aim Few studies have evaluated risk factors for short-term re-bleeding in patients with colonic diverticular bleeding (CDB). We aimed to reveal risk factors for re-bleeding within a month in patients with CDB. Methods We retrospectively analyzed clinical course of patients with CDB diagnosed at 10 institutions between 2015 and 2019. Risk factors for re-bleeding within a month were assessed by Cox proportional hazards models. Results Among 370 patients, 173 (47%) patients had been under the use of antithrombotic agents (ATs) and 34 (9%) experienced re-bleeding within a month. Multivariate analysis revealed that the use of ATs was an independent risk factor for re-bleeding within a month (HR 2.38, 95% CI 1.10–5.50, p = .028). Furthermore, use of multiple ATs and continuation of ATs were found to be independent risk factors for re-bleeding within a month (HR 3.88, 95% CI 1.49–10.00, p = .007 and HR 3.30, 95% CI 1.23–8.63, p = .019, respectively). Two of 370 patients, who discontinued ATs, developed thromboembolic event. Conclusions Use of ATs was an independent risk factor for short-term re-bleeding within a month in patients with CDB. This was especially the case for the use of multiple ATs and continuation of ATs. However, discontinuation of ATs may increase the thromboembolic events those patients.
Research on Simulations of Tactical Ballistic Missile Interception

Based on equations of TBM at reentry phase,a method of navigation was established to intercept TBM on flank.In other words,the interception is generalized from two-dimensional interception to three-dimensional interception.The effects of the method on overload were analyzed and discussed in theory.Simulation of the TBM interception was given.Compared with generalized K-proportional navigation trajectory,the method is better suited for controlling normal overload caused by random error.
Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells

Significance The extracellular concentration of adenosine triphosphate (ATP) reaches several hundred micromoles in the inflamed tissues or tumor environment. A high concentration of ATP activates P2X7, a purinergic receptor, and induces the formation of a nonselective cation channel, accompanied by reversible phosphatidylserine (PtdSer) exposure, leading to cell lysis. Here, we found that Xk and Vps13a complexed at plasma membranes are indispensable for ATP-induced PtdSer exposure and cell lysis. Patients with McLeod syndrome and chorea-acanthocytosis are known to carry the mutation in XK and VPS13A genes, respectively, suggesting that a defect of XK-VPS13A–mediated phospholipid scrambling is responsible for neuroacanthocytosis, a progressive movement disorder, and cognitive and behavior changes. A high extracellular adenosine triphosphate (ATP) concentration rapidly and reversibly exposes phosphatidylserine (PtdSer) in T cells by binding to the P2X7 receptor, which ultimately leads to necrosis. Using mouse T cell transformants expressing P2X7, we herein performed CRISPR/Cas9 screening for the molecules responsible for P2X7-mediated PtdSer exposure. In addition to Eros, which is required for the localization of P2X7 to the plasma membrane, this screening identified Xk and Vps13a as essential components for this process. Xk is present at the plasma membrane, and its paralogue, Xkr8, functions as a phospholipid scramblase. Vps13a is a lipid transporter in the cytoplasm. Blue-native polyacrylamide gel electrophoresis indicated that Xk and Vps13a interacted at the membrane. A null mutation in Xk or Vps13a blocked P2X7-mediated PtdSer exposure, the internalization of phosphatidylcholine, and cytolysis. Xk and Vps13a formed a complex in mouse splenic T cells, and Xk was crucial for ATP-induced PtdSer exposure and cytolysis in CD25+CD4+ T cells. XK and VPS13A are responsible for McLeod syndrome and chorea-acanthocytosis, both characterized by a progressive movement disorder and cognitive and behavior changes. Our results suggest that the phospholipid scrambling activity mediated by XK and VPS13A is essential for maintaining homeostasis in the immune and nerve systems.
Magnitude and sources of proactive interference in visual memory

ABSTRACT Proactive interference – the disruptive effect of old memories on new learning – is a long-established forgetting mechanism, yet there are doubts about its impact on visual working memory and uncertainty about the kinds of information that cause proactive interference. The present study aimed to assess these issues in three experiments using a modified recent probes task. Participants encoded four target images on each trial and determined whether a probe matched one of those targets. In Experiment 1, probes matching targets from trial N-1 or N-3 damaged responding in relation to a novel probe. Proactive interference was also produced by probes differing in state to a previously experienced target. This was further assessed in Experiments 2 and 3. Here, probes differing in colour to a previous target, or matching the general target category only, produced little proactive interference. Conversely, probes directly matching a prior target, or differing in state information, hindered task performance. This study found robust proactive interference in visual working memory that could endure over multiple trials, but it was also produced by stimuli closely resembling an old target. This challenges the notion that proactive interference is produced by an exact representation of a previously encoded image.
Hedgehog pathway modulation by glypican 3-conjugated heparan sulfate.

Glypicans are a family of cell-surface heparan sulfate proteoglycans that play critical roles in multiple cell signaling pathways. Glypicans consist of a globular core, an unstructured stalk modified with sulfated glycosaminoglycan chains, and a glycosylphosphatidylinositol anchor. Though these structural features are conserved, their individual contribution to glypican function remains obscure. Here, we investigate how glypican-3 (GPC3), which is mutated in Simpson-Golabi-Behmel tissue overgrowth syndrome, regulates Hedgehog signaling. We find that GPC3 is necessary for the Hedgehog response, controlling, surprisingly, a downstream signal transduction step. Purified GPC3 ectodomain rescues signaling when artificially recruited to the surface of GPC3-deficient cells, but has dominant negative activity when unattached. Strikingly, the purified stalk, modified with heparan sulfate but not chondroitin sulfate, is necessary and sufficient for activity. Our results demonstrate a novel function of GPC3-associated heparan sulfate, and provide a framework for the functional dissection of glycosaminoglycans by in vivo biochemical complementation.
A Source-Free Domain Adaptive Polyp Detection Framework With Style Diversification Flow

The automatic detection of polyps across colonoscopy and Wireless Capsule Endoscopy (WCE) datasets is crucial for early diagnosis and curation of colorectal cancer. Existing deep learning approaches either require mass training data collected from multiple sites or use unsupervised domain adaptation (UDA) technique with labeled source data. However, these methods are not applicable when the data is not accessible due to privacy concerns or data storage limitations. Aiming to achieve source-free domain adaptive polyp detection, we propose a consistency based model that utilizes Source Model as Proxy Teacher (SMPT) with only a transferable pretrained model and unlabeled target data. SMPT first transfers the stored domain-invariant knowledge in the pretrained source model to the target model via Source Knowledge Distillation (SKD), then uses Proxy Teacher Rectification (PTR) to rectify the source model with temporal ensemble of the target model. Moreover, to alleviate the biased knowledge caused by domain gaps, we propose Uncertainty-Guided Online Bootstrapping (UGOB) to adaptively assign weights for each target image regarding their uncertainty. In addition, we design Source Style Diversification Flow (SSDF) that gradually generates diverse style images and relaxes style-sensitive channels based on source and target information to enhance the robustness of the model towards style variation. The capacities of SMPT and SSDF are further boosted with iterative optimization, constructing a stronger framework SMPT++ for cross-domain polyp detection. Extensive experiments are conducted on five distinct polyp datasets under two types of cross-domain settings. Our proposed method shows the state-of-the-art performance and even outperforms previous UDA approaches that require the source data by a large margin. The source code is available at github.com/CityU-AIM-Group/SFPolypDA.
An Evaluation of the Signs of Nipple Trauma Associated With Breastfeeding: A Delphi Study

Background: No consensus on the definitions of nipple trauma has been reported. Thus, different individuals assess identical events inconsistently. Research Aims: To establish clear definitions for the seven signs of nipple trauma related to breastfeeding—erythema, swelling, scabbing, blistering, fissure, purpura, and peeling—and to reach consensus among multiple specialists. Methods: We implemented a longitudinal, prospective survey design using a 3-step Delphi method. In the first survey we targeted specialists in breastfeeding, dermatology, and cosmetics, and we presented images as representative cases during a set of semi-structured interviews. In the second and third surveys, we questioned midwives who were certified as “advanced midwives” through mail, using a questionnaire prepared based on the results of the first survey. The agreement criteria of this study were as follows: (1) a median of 2.0 or below; (2) an interquartile range of 1.0 or below; and (3) at least 51% showing responses of either “strongly agree” or “agree. ” Results: Based on the responses of the 42 experts, we reached a consensus on 48 items: eight related to erythema, 10 to swelling, nine to scabbing, seven to blistering, seven to fissure, four to purpura, and three to peeling. We then classified these items based on the condition or possible mechanism of the wound. Finally, we developed an observation tool: “seven signs of nipple trauma associated with breastfeeding,” along with images. Conclusions: Application of this tool for breastfeeding support could improve objectivity in observing and evaluating nipple trauma.
Ternstroenol F: a new pentacyclic triterpenoid saponin isolated from the Australian rainforest plant Ternstroemia cherryi

Abstract A detailed close phytochemical investigation of the fruits of Ternstroemia cherryi led to the isolation and identification of the minor metabolite, ternstroenol F, which possessed the usual barrigenol-like terpenoid backbone. The notable difference was that this minor metabolite had the 2(E)-4(Z)-6(E)-decatrienoic acid forming an ester bond at C-22 of the oleanane backbone. Ternstroenol F was evaluated for its inhibitory effects on NO inhibition, cell viability and TNF- α release in RAW 264.7 macrophages, displaying an IC50 values of 0.23, 0.81 and 1.84 µM respectively. Graphical Abstract
‘You’re just there, alone in your room with your thoughts’: a qualitative study about the psychosocial impact of the COVID-19 pandemic among young people living in the UK

Objectives Adolescents and young adults have been greatly affected by quarantine measures during the COVID-19 pandemic, but little is understood about how restrictions have affected their well-being, mental health, and social life. We therefore aimed to learn more about how UK quarantine measures affected the social lives, mental health and well-being of adolescents and young adults. Design Qualitative interview study. The data were analysed using reflexive thematic analysis, with particular attention paid to contextual factors (such as age, gender, ethnicity and health status) when analysing each individual transcript. Setting Data collection took place remotely across the UK via audio or video call, between June 2020 and January 2021. Participants We conducted semi-structured interviews with 37 participants (aged 13–24 years) to elicit their views. Results Authors generated four themes during the qualitative analysis: (a) concerns about disruption to education, (b) missing social contact during lockdown, (c) changes to social relationships and (d) improved well-being during lockdown. Many participants said they struggled with a decline in mental health during the pandemic, lack of support and concern about socialising after the pandemic. However, some participants described experiences and changes brought on by the pandemic as helpful, including an increased awareness of mental health and feeling more at ease when talking about it, as well as stronger relationship ties with family members. Conclusions Findings suggest that young people may have felt more comfortable when talking about their mental health compared with prepandemic, in part facilitated by initiatives through schools, universities and employers. However, many were worried about how the pandemic has affected their education and social connections, and support for young people should be tailored accordingly around some of these concerns.
Infrared Photoactivation Boosts Reporter Ion Yield in Isobaric Tagging.

Isobaric tagging facilitates multiplexed experiments that can determine sequences and relative amounts of peptides in biological samples using tandem mass spectrometry (MSn). Limited reporter ion generation limits quantitative accuracy and precision. As reporter ions are susceptible to unintended fragmentation and scattering by high-energy collisions, we activated peptides with IR photons and prevented successive dissociation of generated reporter ions with ion parking, which altogether boosted reporter ion yield by up to 55%. Even so, unintended co-isolation of contaminating peaks in MS2 experiments distorts reporter ion intensities and can distort quantitative information. MS3 experiments address contamination by generating reporter ions via collisional activation (HCD) of one or more peptide product ions rather than the isolated peptide precursor ion. Because HCD performance is related to m/z, activation of multiple synchronously isolated product ions generates less than optimal reporter ion intensities. In this work, we show that using infrared multiphoton dissociation, which is not dependent on m/z, to generate reporter ions from 10 synchronously isolated peptide product ions results in a 2.4-fold increase in reporter ion intensities, significantly enhancing the sensitivity and dynamic range of quantitation via isobaric tagging.
Susceptibility of the Western Honey Bee Apis mellifera and the African Stingless Bee Meliponula ferruginea (Hymenoptera: Apidae) to the Entomopathogenic Fungi Metarhizium anisopliae and Beauveria bassiana

Abstract This study assessed the nontarget effect of entomopathogenic fungi on the Western honey bee Apis mellifera L. and the African stingless bee Meliponula ferruginea Cockrell (Hymenoptera: Apidae). Pathogenicity of five Metarhizium anisopliae (ICIPE 7, ICIPE 20, ICIPE 62, ICIPE 69, and ICIPE 78) (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and one of Beauveria bassiana (ICIPE 284) (Balsamo) Vuillemin (Hypocreales: Cordicipitaceae) isolates were evaluated on bees at 108 conidia/ml. Conidial acquisition was evaluated immediately after exposure. Apis mellifera acquired more conidia (2.8 × 104–1.3 × 105 conidia per bee) compared to M. ferruginea (1.1 × 104–2.3 × 104 conidia per bee). In the bioassay with A. mellifera, ICIPE 7, ICIPE 20, and ICIPE 69 moderately reduced the survival by 16.9, 17.4, 15.3%, with lethal times LT10 = 7.4, 7.6, 8.1 d and LT25 = 8.7, 10.0, 9.9 d, respectively. The three isolates caused A. mellifera mycosis of 11.6–18.5%. None of the isolates had a significant effect on M. ferruginea. The tested isolates are nontoxic to bees according to the International Organization of Biological Control (IOBC) classification. However, the effect of ICIPE 7, ICIPE 20, and ICIPE 69 merits further studies on bee colonies, especially those of A. mellifera, under field conditions. Graphical Abstract
Characterized Gene Repertoires and Functional Gene Reference for Forensic Entomology: Genomic and Developmental Transcriptomic Analysis of Aldrichina grahami (Diptera: Calliphoridae)

Abstract Many flies of Diptera are common entomological evidence employed in forensic investigation. Exploring the existence of inter- and intra-species genomic differences of forensically relevant insects is of great importance. Aldrichina grahami is a common blow fly species of forensic importance. The present study characterized the gene repertoires of A. grahami, and provides insights into issues related to forensic entomology, such as necrophagous behavior, gene family features, and developmental patterns. Gene families were clustered and classified according to their function in different aspects of the necrophagous lifestyle, generating several gene repertoires. The genes under positive selection pressure and evolutionary changes were screen and identified. Moreover, genes that exhibited potential prediction value in the post mortem interval (PMI) estimation and development of immature stages were subjected to analysis based on the developmental transcriptome. Related insect species were compared at the genomic level to reveal the genes associated with necrophagous behaviors. The expression of selected genes in separated repositories was verified using qPCR. This work was conducted using a high-quality chromosome-level genome assembly of A. grahami and its developmental transcriptome. The findings will facilitate future research on A. grahami and the other forensically important species.
A Control Strategy for Parallel Operation of Multi-inverters in Microgrid

For multi-inverters parallel operation in microgrid,the output impedance of inverters and the line impedance between the inverters and point of common coupling are always different,which can result in big circulating current and lower precision of power sharing among inverters.The traditional droop control strategy and the influence of output impedance of the inverters were analyzed,and a droop control strategy based on voltage and current double-loop for multi-inverters parallel operation in microgrid was proposed by introduction of inductive virtual impedance.The introduced virtual impedance made it possible that the output impedance is only depended on the filtering inductor.The effect of the output resistance of parallel inverters was reduced.Considering the effect of line impedance,an novel improved droop control strategy was proposed.By modifying the droop coefficients,the effect of line impedance on paralleling current sharing was reduced and the system performance of parallel inverters was improved.Both the simulation and experimental results demonstrate that the control strategy is correct and effective.