Miracle-dz/UniFAR-FTUs huggingface dataset:
“He’s Back so I’m Not Alone”: The Impact of Deep Brain Stimulation on Personality, Self, and Relationships in Parkinson’s Disease

Deep brain stimulation (DBS) for Parkinson’s disease successfully alleviates motor symptoms, but unanticipated changes in personality, self, and relationships can occur. Little is known about how these nonmotor outcomes affect patients and families. We prospectively examined the experience and meaning of DBS-related changes in personality and self for patients and caregivers. In-depth, semi-structured interviews were conducted with 22 participants (11 patient–caregiver dyads) before and 9 months after DBS and analyzed using thematic analysis. We identified three themes present prior to DBS that reflected a time of anticipation, while three themes present after DBS reflected a process of adjustment. Participants noted both positive and negative personality changes, with some, but not all, attributing them to the stimulation. The risk of stimulation-related personality change should be weighed against the procedure’s motor benefits and considered in the context of disease- and medication-related personality changes. Clinical implications including perioperative education and follow-up management are discussed.
PDX1−/NKX6.1+ progenitors derived from human pluripotent stem cells as a novel source of insulin‐secreting cells

Beta cell replacement strategies are a promising alternative for diabetes treatment. Human pluripotent stem cells (hPSCs) serve as a scalable source for producing insulin‐secreting cells for transplantation therapy. We recently generated novel hPSC‐derived pancreatic progenitors, expressing high levels of the transcription factor NKX6.1, in the absence of PDX1 (PDX1−/NKX6.1+). Herein, our aim was to characterize this novel population and assess its ability to differentiate into insulin‐secreting beta cells in vitro.
Implantation

Mus; B, the actual condition in Cavia; C, the Site of implantation in both Cases. Eegard- hitherto accepted idea of the condition in Cavia (Selenka); T, Trager ; [ n g the primary Stages involving the first T.S. , trophoblastic sac; E, endoderm.
Circulating Insulin-Like Growth Factor-1 and Risk of Total and 19 Site-Specific Cancers: Cohort Study Analyses from the UK Biobank

Background: Insulin-like growth factor-1 (IGF-1) has been implicated in several malignancies, but few studies have examined multiple cancers simultaneously. We sought to conduct systematic assessments of the association between IGF-1 and cancer risk. Methods: We conducted a prospective analysis between IGF-1 and incident total and 19 site-specific cancers among 412,645 individuals enrolled in the UK Biobank with follow-up to 2016. IGF-1 was measured using blood samples provided at the baseline examination. HR and 95% confidence interval (CI) were calculated with multivariable-adjusted Cox models with IGF-1 modeled both in sex-specific quintiles and continuously. Results: Participants were followed for a median of 7.2 years. We observed positive associations between circulating IGF-1 and overall cancer risk for both men (HR = 1.03 per 5-nmol/L increment in IGF-1; 95% CI, 1.01–1.06) and women (HR = 1.03; 95% CI, 1.01–1.06). For specific sites, we observed positive associations for breast (HR = 1.10; 95% CI, 1.07–1.14), prostate (1.09; 95% CI, 1.05–1.12), colorectum (1.07; 95% CI, 1.02–1.11), melanoma (1.08; 95% CI, 1.01–1.15), kidney (1.10; 95% CI, 1.00–1.20), and thyroid (1.22; 95% CI, 1.05–1.42) and inverse associations for lung (0.91; 95% CI, 0.86–0.96), ovaries (0.86; 95% CI, 0.77–0.95), head and neck (0.90; 95% CI, 0.82–0.99), and liver (0.32; 95% CI, 0.26–0.38). The inverse association between IGF-1 and lung cancer was observed only in ever-smokers (HRever-smoker = 0.88 vs. HRnever-smoker = 1.14; Pinteraction = 0.0005). Analyses comparing extreme quintiles were consistent. Conclusions: IGF-1 is modestly associated with increased risk of total cancer in both men and women but demonstrated divergent associations for site-specific cancers. Impact: Our study suggests that IGF-1 could serve as a target for cancer prevention or treatment.
Chemical Composition, Antimicrobial Properties of Siparuna guianensis Essential Oil and a Molecular Docking and Dynamics Molecular Study of its Major Chemical Constituent

The essential oil of Siparuna guianensis was obtained by hydrodistillation. The identification of the chemical compounds was performed by gas chromatography coupled with mass spectrometry (GC/MS). Antimicrobial activity was investigated for four microorganisms: Streptococcus mutans (ATCC 3440), Enterococcus faecalis (ATCC 4083), Escherichia coli (ATCC 25922), and Candida albicans (ATCC-10231). The studies of doping and molecular dynamics were performed with the molecule that presented the highest concentration of drug–target proteins, 1IYL (C. albicans), 1C14 (E. coli), 2WE5 (E. faecalis), and 4TQX (S. mutans). The main compounds identified were: Curzerene (7.1%), γ-Elemene (7.04%), Germacrene D (7.61%), trans-β-Elemenone (11.78%), and Atractylone (18.65%). Gram positive bacteria and fungi were the most susceptible to the effects of the essential oil. The results obtained in the simulation showed that the major compound atractylone interacts with the catalytic sites of the target proteins, forming energetically favourable systems and remaining stable during the period of molecular dynamics.
Formation and Maturation of the Phagosome: A Key Mechanism in Innate Immunity against Intracellular Bacterial Infection

Phagocytosis is an essential mechanism in innate immune defense, and in maintaining homeostasis to eliminate apoptotic cells or microbes, such as Mycobacterium tuberculosis, Salmonella enterica, Streptococcus pyogenes and Legionella pneumophila. After internalizing microbial pathogens via phagocytosis, phagosomes undergo a series of ‘maturation’ steps, to form an increasingly acidified compartment and subsequently fuse with the lysosome to develop into phagolysosomes and effectively eliminate the invading pathogens. Through this mechanism, phagocytes, including macrophages, neutrophils and dendritic cells, are involved in the processing of microbial pathogens and antigen presentation to T cells to initiate adaptive immune responses. Therefore, phagocytosis plays a role in the bridge between innate and adaptive immunity. However, intracellular bacteria have evolved diverse strategies to survive and replicate within hosts. In this review, we describe the sequential stages in the phagocytosis process. We also discuss the immune evasion strategies used by pathogens to regulate phagosome maturation during intracellular bacterial infection, and indicate that these might be used for the development of potential therapeutic strategies for infectious diseases.
Deep Spatial Transformation for Pose-Guided Person Image Generation and Animation

Pose-guided person image generation and animation aim to transform a source person image to target poses. These tasks require spatial manipulation of source data. However, Convolutional Neural Networks are limited by the lack of ability to spatially transform the inputs. In this article, we propose a differentiable global-flow local-attention framework to reassemble the inputs at the feature level. This framework first estimates global flow fields between sources and targets. Then, corresponding local source feature patches are sampled with content-aware local attention coefficients. We show that our framework can spatially transform the inputs in an efficient manner. Meanwhile, we further model the temporal consistency for the person image animation task to generate coherent videos. The experiment results of both image generation and animation tasks demonstrate the superiority of our model. Besides, additional results of novel view synthesis and face image animation show that our model is applicable to other tasks requiring spatial transformation. The source code of our project is available at https://github.com/RenYurui/Global-Flow-Local-Attention.
Absorbance detection in multi-reflection microfluidic channels using a commercial microplate reader system.

Absorbance detection is often prohibited in microfluidic channels due to the limited optical path length available in these systems. However, this optical distance may be significantly increased by guiding the probing light beam along the channel length via multiple reflections by patterned metallic surfaces. In this work, we demonstrate enhanced absorbance detection in glass microfluidic channels using a commercial microplate reader based on this principle yielding detection limits comparable to that measured on standard microwell plates. This improvement in detectability was realized through careful optimization of the mirror lengths and locations combined with appropriate design of a microchip holder to suitably position the microchannels in the microplate reader. Additionally, it was determined that the angle by which our device was tilted relative to the horizontal plane played an important role in this optimization. For an optimum choice of parameters accessible with our design, the sensitivity of our absorbance measurements in a 30 µm deep channel was improved by as much as 52-fold, raising this quantity to about 84% of the corresponding value realized for 75 µL samples placed within 7 mm i.d. standard cylindrical microwells. Quantitative ELISAs employing the absorbance detection method were demonstrated on the noted multi-reflection microchip device for assessing West Nile viral IgM antibody levels in human serum samples yielding analyte detection limits comparable to that measured on standard microwell plates.
Outcomes of a health informatics technology-supported behavioral activation training for care managers in a collaborative care program.

INTRODUCTION Health informatics-supported strategies for training and ongoing support may aid the delivery of evidence-based psychotherapies. The objective of this study was to describe the development, implementation, and practice outcomes of a scalable health informatics-supported training program for behavioral activation for patients who screened positive for posttraumatic stress disorder and/or bipolar disorder. METHOD We trained 34 care managers in 12 rural health centers. They used a registry checklist to document the delivery of 10 behavioral activation skills for 4,632 sessions with 455 patients. Care managers received performance feedback based on registry data. Using encounter-level data reported by care managers, we described the implementation outcomes of patient reach and care manager skill adoption. We used cross-classified multilevel modeling to explore variation in skill delivery accounting for patient characteristics, provider characteristics, and change over time. RESULTS Care managers engaged 88% of patients in behavioral activation and completed a minimum course for 57%. The average patient received 5.9 skills during treatment, with substantial variation driven more by providers (63%) than patients (29%). Care managers significantly increased the range of skills offered to patients over time. DISCUSSION The registry-based checklist was a feasible training and support tool for community-based providers to deliver behavioral activation. Providers received data-driven performance feedback and demonstrated skill improvement over time, promoting sustainment. Future research will examine patient-level outcomes. Results underscore the potential public health impact of a simple registry-based skills checklist coupled with a scalable remote training program for evidence-based psychotherapy. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
An Empirical Model of Stomatal Conductance

An empirical model of stomatal conductance is presented. The predictions of conductance correlate with experimental determinations of the responses to several kinds of short-term and long-term perturbations, including irradiance, temperature, CO2 and O2 partial pressures, and leaf age and nitrogen nutrition. The model is not mechanistic and cannot account for the observed functioning of stomata independent of mesophyll. Nevertheless its success in predicting responses in intact leaves means that the mathematical expressions used must be analogous to those describing actual functioning.
Evaluating triple ICS/LABA/LAMA therapies for COPD patients: a network meta-analysis of ETHOS, KRONOS, IMPACT, and TRILOGY studies

ABSTRACT Objectives In some studies comparing triple with dual combination therapies in COPD there might be a possible effect of inhaler bias resulting from different inhaler devices being used in comparator arms. The aim of this study was a quantitative synthesis by considering the studies that directly compared triple ICS/LABA/LAMA vs. either dual LABA/LAMA or ICS/LABA therapies administered at fixed-dose combination (FDC) via the same inhaler device. Methods A network meta-analysis was performed to assess the efficacy/safety impact of triple ICS/LABA/LAMA FDC compared with dual LABA/LAMA and ICS/LBA FDCs administered via the same inhaler device in COPD patients. The treatment ranking was reported via the surface under the cumulative ranking curve analysis (SUCRA). Results Data obtained from 21,909 COPD patients were extracted from the ETHOS, KRONOS, IMPACT, and TRILOGY studies, the only that fulfilled the strict inclusion criteria of this research. The weighted efficacy/safety profile resulting from SUCRA provided the following ranking in patients with low eosinophil count: ICS/LABA/LAMA>LABA/LAMA≫ICS/LABA; whereas in patients with high eosinophil count the ranking was as follows: ICS/LABA/LAMA>LABA/LAMA>ICS/LABA FDC. Conclusion Triple ICS/LABA/LAMA FDC and dual LABA/LAMA or ICS/LABA FDCs are characterized by specific efficacy/safety profiles in agreement with the level of blood eosinophil count at baseline.
The Risk of New Onset Dementia And/Or Alzheimer's Disease Among Prostate Cancer Patients Treated with Androgen Deprivation Therapy: A Systematic Review and Meta-analysis.

INTRODUCTION Androgen deprivation therapy (ADT) is a standard therapy for some localized and almost all metastatic prostate cancer (PCa) patients. Although several clinical cohort studies have identified an impact of ADT on the cognitive function, the previous reviews were not able to perform a well designed quantitative synthesis to summarize the risk of dementia and/or Alzheimer's disease (AD). Consequently, there is still a lack of systematic review and meta-analysis regarding the impact of this risk with including more recent studies. METHOD AND MATERIAL We conducted a systematic review and meta-analysis of the literature assessing the differential incidence of dementia and/or AD as outcomes in PCa patients who received ADT compared to PCa patients who did not. We queried Pubmed and Web of Science database from 1th to 3th January, 2020. We used random or fixed-effects meta-analytic models in the presence or absence of heterogeneity per the I2 statistic, respectively. We performed six meta-analyses for all-cause dementia, AD, and all-cause dementia or AD according to the duration of ADT (≤12 or ˃12 months). RESULTS Fourteen studies after considering inclusion and exclusion criteria were selected. Nine of them reported all-cause of dementia (i.e., all types of dementia including AD), eight studies reported AD. Five studies assessed these outcomes, according to the duration of ADT. The risk of new-onset dementia (all-cause) and AD was higher in PCa patients who received ADT compared to those who did not (HR=1.21, 95%CI 1.11-1.33, and HR=1.16, 95%CI 1.09-1.24, respectively). The risk of dementia (all-cause) was higher in PCa patients who received ADT for more than 12-month (HR=1.36, 95%CI 1.07-1.72), however, for those who had less than 12-month ADT exposure was not statistically significant 1.06 (95% CI: 0.77-1.28). There was not any association between the ADT duration and the risk of AD; ≤12 (HR= 1.21 95% CI: 0.97-1.51) and ˃12 (HR= 1.39, 95% CI 0.69-2.79). CONCLUSION Patients who receive ADT for PCa have an increased risk of dementia and/or AD compared to men who do not receive ADT; this was more pronounced when ADT was given longer than 12 months.
Chronotypes and trauma reactions in children with ADHD in home confinement of COVID-19: full mediation effect of sleep problems

ABSTRACT This study aimed to investigate the relationship between chronotype preference/sleep problems and symptom severity of children with Attention Deficit Hyperactivity Disorder (ADHD) during the COVID-19 outbreak and to assess the chronotype preference/sleep problems that may play a mediating role in the relationship between the reactions to trauma and severity of ADHD symptoms. The sample of this single-center cross-sectional study consisted of 76 children with ADHD and their parents. Trauma symptoms were evaluated with the Children’s Impact of Event Scale (CRIES-8); sleep habits were assessed using the Children’s Sleep Habits Questionnaire (CSHQ); and chronotype was assessed using the Children’s Chronotype Questionnaire (CCQ). There were significant differences in CRIES-8 and CSHQ scores between the eveningness type group and the non-eveningness type group. The CRIES-8 scores of children with ADHD were related to the CCQ and CSHQ scores and severity of ADHD symptoms. In mediation analyses, sleep problems were found to be the full mediating factor in the relationship between CRIES-8 scores and severity of ADHD symptoms and the relationship between CCQ scores and the severity of ADHD symptoms. Our findings indicate that chronotype plays an important role on the negative effects of home confinement of ADHD children during the COVID-19 outbreak. The role of the full mediator of sleep problems in the path from cognition to the behavior of young ADHD and non-ADHD children confined to the home environment during the pandemic period requires further assessment.
CRISPR/Cas9 Delivery Potentials in Alzheimer’s Disease Management: A Mini Review

Alzheimer’s disease (AD) is the most common dementia disorder. While genetic mutations account for only 1% of AD cases, sporadic AD resulting from a combination of genetic and risk factors constitutes >90% of the cases. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated protein (Cas9) is an impactful gene editing tool which identifies a targeted gene sequence, creating a double-stranded break followed by gene inactivation or correction. Although CRISPR/Cas9 can be utilized to irreversibly inactivate or correct faulty genes in AD, a safe and effective delivery system stands as a challenge against the translation of CRISPR therapeutics from bench to bedside. While viral vectors are efficient in CRISPR/Cas9 delivery, they might introduce fatal side effects and immune responses. As non-viral vectors offer a better safety profile, cost-effectiveness and versatility, they can be promising for the in vivo delivery of CRISPR/Cas9 therapeutics. In this minireview, we present an overview of viral and non-viral vector based CRISPR/Cas9 therapeutic strategies that are being evaluated on pre-clinical AD models. Other promising non-viral vectors that can be used for genome editing in AD, such as nanoparticles, nanoclews and microvesicles, are also discussed. Finally, we list the formulation and technical aspects that must be considered in order to develop a successful non-viral CRISPR/Cas9 delivery vehicle.
Incidence of postpartum and neonatal illnesses and utilization of healthcare services in rural communities in southern Ethiopia: A prospective cohort study

Although improving postpartum and neonatal health is a key element of the Ethiopian health extension program, the burdens of postpartum and neonatal illnesses and healthcare-seeking in rural communities in Ethiopia are poorly characterized. Therefore, we aimed to assess the incidence and risk factors for these illnesses and measure the utilization of healthcare services. We conducted a prospective cohort study of 784 postpartum women and their 772 neonates in three randomly selected kebeles in rural southern Ethiopia. Eight home follow-up visits were conducted during the first 42 postpartum days, and six neonate follow-ups were conducted at the same home over the first 28 days of life. The Prentice, Williams, and Peterson’s total time Cox-type survival model was used for analysis. We recorded 31 episodes of postpartum illness per 100 women-weeks (95% confidence interval [CI]: 30%, 32%) and 48 episodes of neonatal illness per 100 neonate-weeks (95% CI: 46%, 50%). Anemia occurred in 19% of women (95% CI: 17%, 22%) and low birth weight (<2,500g) in 15% of neonates (95% CI: 13%, 18%). However, only 5% of postpartum women (95% CI: 4%, 7%) and 4% of neonate (95% CI: 3%, 5%) reported utilizing healthcare services. Walking over 60 minutes to access healthcare was a factor of both postpartum illnesses (AHR = 2.61; 95% CI: 1.98, 3.43) and neonatal illnesses (AHR = 2.66; 95% CI: 2.12, 3.35)). Birth weight ≥2500g was identified factor of neonatal illnesses (AHR = 0.39; 95% CI: 0.33, 0.46). Compared with younger mothers, older mothers with sick newborns (AHR = 1.22; 95% CI: 1.00, 1.50) or postpartum illnesses (AHR = 1.40; 95% CI: 1.03, 1.89) were more likely to seek healthcare. Reasons for not utilizing healthcare services included a belief that the illnesses were not serious or would resolve on their own, little confidence in the healthcare institutions, and the inability to afford the cost. The burden of postpartum and neonatal illnesses in rural communities of southern Ethiopia remains high. Unfortunately, few participants utilized healthcare services. We recommend strengthening the health system that enables identifying, managing, treating, and referring maternal and neonatal illnesses and provide reasonable healthcare at the community level.
Multi-Dimensional Social Relations and Mental Health among Rural Older Adults

To date, knowledge of the association between social-relational patterns and health among rural elders in non-Western social and cultural contexts is scant. Using regional population data in rural South Korea, this study examines comprehensive social relations among rural elders, and the extent to which heterogeneous patterns of social relations were associated with cognitive impairment and depression. Using comprehensive social-relational indicators with (a) the structure of social relations (b) the quality of social relations, we applied latent class analysis identified four subgroups of social relations among rural older adults: Restricted, Spouse Focused-Ambivalent, Community Engaged-Positive, and Diverse. We found that the Community-Engaged group was less likely to be depressed as well as have co-ocurring problems of cognitive impairment and depression.
Elucidating the role of polygalacturonase genes in strawberry fruit softening.

To disentangle the role of polygalacturonase (PG) genes in strawberry softening, the two PG genes most expressed in ripe receptacle, FaPG1 and FaPG2, were down-regulated. Transgenic ripe fruits were firmer than wild type when PG genes were silenced individually. Simultaneous silencing of both PG genes by transgene stacking did not result in an additional increase in firmness. Cell walls from ripe fruits were characterized by a carbohydrate microarray. Higher signals of homogalacturonan and rhamnogalacturonan I pectin epitopes in polysaccharide fractions tightly bound to the cell wall were observed in the transgenic genotypes suggesting a lower pectin solubilization. At the transcriptomic level, the suppression of FaPG1 or FaPG2 alone induced few transcriptomic changes in the ripe receptacle, but the amount of differentially expressed genes increased notably when both genes were silenced. Many genes encoding cell wall modifying enzymes were down-regulated. The expression of a putative high affinity potassium transporter was induced in all transgenic genotypes, indicating that cell wall weakening, and loss of cell turgor could be linked. These results suggest that, besides the disassembly of pectins tightly linked to the cell wall, PGs could play other roles in strawberry softening, such as the release of oligogalacturonides exerting a positive feedback in softening.