Miracle-dz/UniFAR-FTUs huggingface dataset:
Exploring biofertilizer potential of plant growth-promoting rhizobacteria candidates from different plant ecosystems

Abstract. Agustiyani D, Dewi TK, Laili N, Nditasari A, Antonius S. 2021. Exploring biofertilizer potential of plant growth-promoting rhizobacteria candidates from different plant ecosystems. Biodiversitas 22: 2691-2698. Plant growth-promoting rhizobacteria (PGPR) have been widely used as inoculants to increase the growth of crops. This study aims to evaluate the effective PGPR strains according to their capability in various plant growth-promoting activities in vitro. Ten rhizosphere soil samples were obtained from several plant ecosystems in Bangkinang, Kampar, Sumatra Island, Indonesia. A total of 42 bacteria were isolated and tested for three plant growth–promoting activities i.e., phosphate solubilization, indole-3-acetic acid (IAA) production, and N fixation. Out of 42 isolates, 26 were positive for phosphate solubilization, 11 were positive for IAA production, and five were positive for N fixation. The qualitative and quantitative analysis of plant growth–promoting activities revealed that the highest phosphate solubilizing isolate was PK.4. 2 (SI 4.33); the highest IAA-producing isolate was I.4. 2 (73.1 ppm); while the highest N-fixing ability was NFB.1. 1. According to the seed bioassay results, the shoot and root length of bok choy (Brassica rapa) seedlings were significantly enhanced with the PGPR isolates treatment. The highest shoot length (2.53 cm) was observed among seeds treated with I.4. 2 isolate, followed by PK.6. 1 (2.45 cm) and I.5. 3 (2.07 cm). The NFB.1. 1 isolate promoted the longest root length (6.4 cm), although this was similar with I.5. 3 isolate (6.11 cm). Two isolates that showed significant plant growth–promoting activities were analyzed using 16S rDNA sequences. The two isolates had a close evolutionary relationship with genus Sinomonas strain Cw 108 (I.4.2) and Arthrobacter (I.5.3.).
Multi-key Private Matching for Compute

We extend two-party private set union for secure computation, by considering matching between records having multiple identiﬁers (or keys), for example email and phone. In the classical setting of this problem, two parties want to perform various downstream computations on the union of two datasets. The union is computed by joining two datasets with the help of a single agreed upon identiﬁer, say email. By extending this to joining records with multiple identiﬁers, we bring it much closer to real world uses where the match rate and match quality can be greatly improved by considering multiple identiﬁers. We introduce an extension to the Private-ID protocol [3] which outputs a full outer join (union) of two datasets by a match logic that can join rows containing multiple identiﬁers. We also introduce new techniques for privately sharding the protocol across multiple servers. Both constructions are based on Decisional Diﬃe–Hellman (DDH) assumptions.
Complicated grief: a systematic review of the prevalence, diagnosis, risk and protective factors in the adult population of Spain.

espanolEl objetivo principal de este trabajo fue revisar el estado de la investigacion psicopatologica del duelo complicado en poblacion adulta de Espana. Una busqueda sistematica en PsycINFO, PsycArticles, PTSDpubs y PSICODOC identifico 13 estudios en los que, en conjunto, se habia evaluado a 1,655 adultos. Los resultados de esta revision ponen de manifiesto que no existe consenso en el diagnostico de duelo complicado, en el uso de instrumentos de deteccion ni en los factores de riesgo y proteccion del duelo complicado. La prevalencia media ponderada basada en 6 de los estudios revisados fue de 21,53%. Tomando en consideracion el tipo de instrumento de medida del duelo utilizado, se obtuvo una prevalencia de 7,67-10,68% en aquellos estudios que utilizaban instrumentos diagnosticos y de 28,77% en los instrumentos sintomaticos. Los resultados indican que el duelo complicado se relaciona con: un menor nivel socioeconomico y situacion laboral desfavorable, la perdida de un hijo o conyuge, menor edad del fallecido, vulnerabilidad psicologica previa, consumo de psicofarmacos y comorbilidad con otros trastornos. El apoyo social, los cuidados paliativos, las estrategias de afrontamiento centradas en el problema, el empleo de actividades agradables y la trascendencia o espiritualidad se presentan como factores protectores. EnglishThe main objective of this research was to review the status of the psychopathological investigation of complicated grief in adult population of Spain. A systematic review on PsycINFO, PsycArticles, PTSDpubs, and PSICODOC databases identified 13 studies in which, overall, 1.655 adults had been evaluated. The results of this review show that there is no consensus in the diagnosis of complicated grief, in the use of detection instruments, or in the risk and protective factors for complicated grief. The weighted mean prevalence based on 6 of the reviewed studies was 21.53%. Taking into account the type of grief measurement used, a prevalence of 7.67-10.68% was obtained in those studies that used diagnostic instruments and 28.77% in the ones using symptomatic instruments. The results indicate that complicated grief is related to: a lower socioeconomic level and unfavorable work situation, the loss of a child or spouse, younger age of the deceased, previous psychological vulnerability, the use of psychotropic medication and comorbidity with other disorders. Social support, palliative care, problem-centered coping strategies, the use of pleasant activities, and transcendence or spirituality are shown as protective factors.
The in vitro antifungal activities of methanolic and ethanolic leaf extracts against Aspergillus niger , Alternaria alternata , Curvularia lunata Trichophyton

Fungal infections caused by , Trichophyton mentagrophytes Aspergillus fumigatus Malassezia pachydermatis , and are among the major contributors to multisystemic health problems such as dermatitis, otitis, and respiratory disorders among humans and animals. This study was conducted to determine the in vitro antifungal activity of leaf crude aqueous and Terminalia catappa ethanolic extracts against these fungal pathogens by measuring the zone of inhibition (ZI) using the agar well diffusion technique. Qualitative phytochemical screening tests were also performed to determine bioactive compounds present in the plant extract. Results show that the plant's crude aqueous (CAE) and ethanolic extracts (CEE) were found to be effective against all test fungi. M. pachydermatis showed susceptibility towards CAE and CEE from T1 (100%), T2 (75%), T3 (50%) and T4 (25%), with the highest mean ZI of 18.33mm and 13.33, respectively. On the other hand, was inhibited by CAE and CEE at T1 (100%), T. mentagrophytes T2 (75%) and T3 (50%) with the highest mean ZI of 9.67mm and 10.33mm, respectively. At the same time, it was observed that had reactive A. fumigatus sensitivity towards CAE and CEE at T1 (100%) and T2 (75%), with the highest mean ZI of 9.33mm and 10.33mm, respectively. Moreover, phytochemical tests showed that the plant's leaf crude extracts contain alkaloids, saponins, and tannins, which could potentially inhibit fungal growth.
Leveraging PDDL to Make Inscrutable Agents Interpretable: A Case for Post Hoc Symbolic Explanations for Sequential-Decision Making Problems

There has been quite a bit of interest in developing explanatory techniques within ICAPS-community for various planning ﬂavors, as evidenced by the popularity of the XAIP workshop in the past few years. Though most existing works in XAIP focus on creating explanatory techniques for native planning-based systems that leverage human-speciﬁed models. While this has led to the development of valuable techniques and tools, our community tends to overlook a very important avenue where the XAIP techniques, particularly ones designed around symbolic human-readable models, could make a practical and immediate impact. Namely to help generate symbolic post hoc explanations for sequential decisions generated through inscrutable decision-making systems, including Reinforcement-Learning and any inscrutable model-based planning/approximate dynamic programming methods. Through this paper, we hope to discuss how we could generate such post hoc explanations. Motivate how one could use the current XAIP techniques to address many explanatory challenges within this realm and also discuss some of the open research challenges that arise when we try to apply our methods within this new application context.
Density Functional Theory Calculations for the Quantum Capacitance Performance of Graphene-Based Electrode Material

With first-principles density functional theory calculations, we demonstrate that quantum capacitance of graphene-based electrodes can be improved by the N-doping, vacancy defects, and adsorbed transition-metal atoms. The enhancement of the quantum capacitance can be contributed to the formation of localized states near Dirac point and/or shift of Fermi level induced by the defects and doping. In addition, the quantum capacitance is found to increase monotonically following the increase of defect concentrations. It is also found that the localized states near Fermi level results in the spin-polarization effect.
Stability and Changes in Traditional and Cyberbullying Perpetration and Victimization in Childhood: The Predictive Role of Depressive Symptoms

Childhood bullying is a public health concern. The stability and changes in children’s bullying profiles should provide useful information about specific risk groups and their developmental patterns. The current longitudinal study examined the stability and changes in bullying involvement in children along with the predictive roles of depressive symptoms in bullying subgroup memberships and transitions. A total of 4,321 Chinese children (55.1% male, Mage = 9.93) participated in self-report assessments at three time points with six-month intervals. Bullying subgroups were identified based on physical, relational, and cyberbullying using latent profile analysis. The transitions patterns were modeled using latent transition analysis. Depressive symptoms were added as a covariate in bullying subgroup memberships and transitions. Five bullying subgroups were found at Time 1 and Time 2: traditional bully-victims, traditional victims, (cyber) bully-victims, (cyber) victims, and noninvolved children, whereas four bullying subgroups were found at Time 3: traditional bully-victims, traditional victims, (cyber) bully-victims, and noninvolved children. Findings suggested that (a) childhood bullying involvement showed varying levels of stability, with noninvolved children displaying the most stability and cyberbullying-involved children displaying the least stability; (b) children reporting more severe depressive symptoms manifested a higher risk of bullying involvement; (c) initially noninvolved children showing depressive symptoms were at a higher risk for becoming traditional victims; and (d) children reporting depressive symptoms found it more difficult to escape from bullying than children without depressive symptoms. The findings offer support for both the cumulative continuity and interactional models of development. The findings also highlight the important the role of depressive symptoms in preventing and ameliorating children’s bullying.
Comparative genomics of the Pseudomonas corrugata subgroup reveals high species diversity and allows the description of Pseudomonas ogarae sp. nov.

Pseudomonas corrugata constitute one of the phylogenomic subgroups within the Pseudomonas fluorescens species complex and include both plant growth-promoting rhizobacteria (PGPR) and plant pathogenic bacteria. Previous studies suggest that the species diversity of this group remains largely unexplored together with frequent misclassification of strains. Using more than 1800 sequenced Pseudomonas genomes we identified 121 genomes belonging to the P. corrugata subgroup. Intergenomic distances obtained using the genome-to-genome blast distance (GBDP) algorithm and the determination of digital DNA–DNA hybridization values were further used for phylogenomic and clustering analyses, which revealed 29 putative species clusters, of which only five correspond to currently named species within the subgroup. Comparative and functional genome-scale analyses also support the species status of these clusters. The search for PGPR and plant pathogenic determinants showed that approximately half of the genomes analysed could have a pathogenic behaviour based on the presence of a pathogenicity genetic island, while all analysed genomes possess PGPR traits. Finally, this information together with the characterization of phenotypic traits, allows the reclassification proposal of Pseudomonas fluorescens F113 as Pseudomonas ogarae sp. nov., nom rev., type strain F113T (=DSM 112162T=CECT 30235T), which is substantiated by genomic, functional genomics and phenotypic differences with their closest type strains.
Synthesis of N-doped carbon dots for highly selective and sensitive detection of metronidazole in real samples and its cytotoxicity studies

ABSTRACT The current investigation reports the synthesis of N-CDs using glucosamine, ascorbic acid, and ethylenediamine precursors by a simple hydrothermal technique. The formation of N-CDs was proved by various characterisation techniques such as X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier-Transform Infrared Spectrophotometer (FT-IR). The optical properties were investigated by fluorescence and UV-vis spectrophotometer. Also, N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. However, the metronidazole serves as an antibiotic against several microbial diseases but also a genotoxic, carcinogenic to the human when used in excessive dosage. The synthesised N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. Besides, the cytotoxicity of the N-CDs was studied to evaluate its toxicity against the HeLa cancer cells. It showed 65.6% cell viability and 34.3% toxicity against the cancerous cells, and similarly 71% of cells viability against H9C2 cells. Thus, the current investigation explores the promising selective sensing of N-CDs against MTZ, along with that, it proved its cytotoxicity against HeLa cancerous cells and non-toxicity against H9C2 cells. The synthesised CDs can be better MTZ sensors and anti-cancer agents on further development at the industrial scale. GRAPHICAL ABSTRACT
The effect of micronutrient supplementation on spermatozoa DNA integrity in subfertile men and subsequent pregnancy rate

Abstract Background Male infertility has been related to an increased sperm DNA fragmentation index (DFI). Nutritional factors may improve sperm nuclear DNA integrity and thus pregnancy rates. Objective: To evaluate the effect of micronutrient supplementation on sperm DNA integrity in subfertile men and subsequent pregnancy rates. Methods In this retrospective comparative study 339 subfertile males were included on whom a sperm chromatin dispersion test (SCD) was performed as a method to detect DNA fragmentation, as well as an initial semen analysis. Of all, n = 162 received a nutritional management program for three months, consisting of two daily capsules of a standardized combined micronutrient formulation together with a guidance to diet modification and to lifestyle changes (study group). Each capsule contained L-carnitine, L-arginine, coenzyme Q10, zinc, vitamin E, folic acid, glutathione, and selenium. The control group consisted of those patients who did not receive the active treatment (n = 177), yet were instructed to engage in a healthy lifestyle, including a modification of their regular diet. The SCD test was repeated for both groups after three months. As part of the routine follow up, pregnancy rate was assessed six months after the second SCD test. Males with complete follow up and healthy female partners (aged 18 to 40 years) where included. Results Data of men with an initial mean DFI of >15% were analyzed first (n = 81;46 study and 35 control patients). After three months, both groups displayed a significant decrease of mean DFI values; however, the mean percent difference was higher in the study group (10.46 ± 1.20 % vs. 5.29 ± 0.57 %; p < .001). Then, the entire population was considered (n = 339). After three months, only the study group displayed a significant decrease of mean DFI initial values (10.48 ± 7.76 % to 6.51 ± 4.61%; p < .001); and the percent difference was higher in the study group (3.97 ± 0.28 % vs. 0.91 ± 0.28 %; p < .001). At six months follow-up, pregnancy rate was higher in the study group (27.78% vs. 15.25%, p = .002). Conclusion Both regimes significantly reduced sperm DNA fragmentation among subfertile men with a DFI >15%; however, when any baseline DFI value was considered, only micronutrient supplementation achieved a better result on DFI and thus pregnancy rate was higher.
Stress-Mediated Reprogramming of Prostate Cancer One-Carbon Cycle Drives Disease Progression

These findings demonstrate that the mitochondrial, but not cytoplasmic, one-carbon cycle has a key role in prostate cancer cell growth and survival and may serve as a biomarker and/or therapeutic target. One-carbon (1C) metabolism has a key role in metabolic programming with both mitochondrial (m1C) and cytoplasmic (c1C) components. Here we show that activating transcription factor 4 (ATF4) exclusively activates gene expression involved in m1C, but not the c1C cycle in prostate cancer cells. This includes activation of methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) expression, the central player in the m1C cycle. Consistent with the key role of m1C cycle in prostate cancer, MTHFD2 knockdown inhibited prostate cancer cell growth, prostatosphere formation, and growth of patient-derived xenograft organoids. In addition, therapeutic silencing of MTHFD2 by systemically administered nanoliposomal siRNA profoundly inhibited tumor growth in preclinical prostate cancer mouse models. Consistently, MTHFD2 expression is significantly increased in human prostate cancer, and a gene expression signature based on the m1C cycle has significant prognostic value. Furthermore, MTHFD2 expression is coordinately regulated by ATF4 and the oncoprotein c-MYC, which has been implicated in prostate cancer. These data suggest that the m1C cycle is essential for prostate cancer progression and may serve as a novel biomarker and therapeutic target. Significance: These findings demonstrate that the mitochondrial, but not cytoplasmic, one-carbon cycle has a key role in prostate cancer cell growth and survival and may serve as a biomarker and/or therapeutic target.
The Role of the Gastrointestinal Tract in Toxigenic Clostridium tetani Infection: A Case-Control Study

ABSTRACT. Tetanus arises from wound contamination with Clostridium tetani, but approximately one fifth of patients have no discernable entry wound. Clostridium tetani is culturable from animal feces, suggesting the gastrointestinal tract could be an endogenous reservoir or direct-entry portal, but human data are lacking. In this study of 101 Vietnamese adults with tetanus and 29 hospitalized control subjects, admission stool samples were cultured for C. tetani. Anti-tetanus toxin antibodies were measured by ELISA. Clostridium tetani toxigenicity was evaluated using polymerase chain reaction and sequencing. Toxigenic C. tetani was cultured from stool samples in 50 of 100 (50%) tetanus cases and 12 of 28 (42.9%) control subjects (P = 0.50), and stool samples of 44 of 85 (52.4%) tetanus cases with clinically identified wounds compared with 6 of 15 (47.6%) patients without clinically identified wounds (P = 0.28). Nine of 12 (75%) control subjects with toxigenic C. tetani in their stool samples lacked protective antibody concentrations. These findings fail to show evidence of an association between gastrointestinal C. tetani and tetanus infection, but emphasize the importance of increasing vaccination coverage.
The Embodiment of an Older Avatar in a Virtual Reality Setting Impacts the Social Motivation of Young Adults

ABSTRACT OBJECTIVES. Socio-emotional selectivity theory implies that an individual’s motives change over their lifespan, starting with a focus on information seeking and shifting toward the motivation of maintaining emotionally meaningful social relationships in old age. The concept of future time perspective serves as an underlying mechanism for this phenomenon. METHODS. This study aimed to capture how social motivation changes as a result of the manipulation of one’s own visual appearance. Thus, the explicit age stereotypes of N = 74 participants were assessed, among other covariates. The following intervention consisted of a virtual reality (VR) scenario in which the experimental group embodied an old age avatar and the control group a young age avatar. RESULTS. Changes in social motivation were assessed using the concept of socio-emotional selectivity based on imagined situational preferences. Results with strong effect sizes indicate that changes in social motivation commonly connected with old age might be caused by visual cues when actively embodying a virtual avatar.
Evaluation of the inhibitory effects of Wuyiencin，a secondary metabolite of Streptomyces albulus CK-15, against Sclerotinia sclerotiorum in vitro.

Sclerotinia sclerotiorum (Lib) de Bary, a destructive fungal pathogen with an extensive host range, causes various diseases with the potential to cause huge economic losses to crops worldwide. Streptomyces species produce secondary metabolites with variable structures and biological activities that offer possible control methods for crop diseases. Herein, we evaluated the inhibitory effects of Wuyiencin, a secondary metabolite of Streptomyces albulus CK-15, against S. sclerotiorum. The results showed that Wuyiencin markedly inhibited mycelial growth and germination, and the formation of sclerotia. It also increased cell membrane permeability, resulting in leakage of intracellular substances in pathogen mycelia. Wuyiencin markedly decreased oxalic acid (OA) content and the activities of polygalacturonase (PG) and pectin methyl-galacturonic (PMG) enzymes. Moreover, it downregulated Nox1, ITL, pph1, Caf1 and sca1, all genes related to growth and infection. Lesions were smaller and less pronounced on soybean (Glycine max (Linn.) Merr. ) leaves pretreated with Wuyiencin in vitro, and the inhibition rate reached 78.36%. The results suggest that Wuyiencin holds promise for the management of diseases caused by S. sclerotiorum, and the findings provide clues on the mechanism of action.
Women and divorce: financial coping from midlife to older age

ABSTRACT Divorce is a life-changing event with financial implications for women. Although large-scale studies have examined the factors associated with financial coping after divorce, little attention has been paid to the lived experience of women over time. In this study, we used mixed methods to examine the financial well-being of divorced women over 20 years from 1996 to 2016. Using data from the Australian Longitudinal Study on Women’s Health [ALSWH], we analyzed women’s ratings of their ability to manage on available income, and their narrative comments about financial coping over eight waves, beginning in midlife (ages 46–51). The ratings improved over time, particularly as women reached peak career in their 50s or 60s or entered retirement. Despite this upward trajectory, financial strain persisted for ~40% of the cohort who faced poor health or diminishing job prospects. We conclude that, although financial hardship often eases over time, women’s early ratings of financial coping predict levels of income security in older age.