Miracle-dz/UniFAR-FTUs huggingface dataset:
Failure of cAMP agonists to activate rescued ΔF508 CFTR in CFBE41o– airway epithelial monolayers

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cyclic AMP‐regulated chloride channel. Mutations in the CFTR gene result in cystic fibrosis (CF). The most common mutation, ΔF508, results in endoplasmic reticulum‐associated degradation (ERAD) of CFTR. ΔF508 CFTR has been described as a temperature‐sensitive mutation that can be rescued following growth at 27°C. In order to study the processing and function of wild‐type and rescued ΔF508 CFTR at the cell surface under non‐polarized and polarized conditions, we developed stable cell lines expressing ΔF508 or wild‐type CFTR. CFBE41o– is a human airway epithelial cell line capable of forming high resistance, polarized monolayers when cultured on permeable supports, while HeLa cells are normally grown under non‐polarizing conditions. Immunoprecipitation, cell surface biotinylation, immunofluorescence, and functional assays confirmed the presence of ΔF508 CFTR at the cell surface in both cell lines after incubating the cells for 48 h at 27°C. However, stimulators of wild‐type CFTR such as forskolin, β2‐adrenergic or A2B‐adenosine receptor agonists failed to activate rescued ΔF508 CFTR in CFBE41o– monolayers. Rescued ΔF508 CFTR could be stimulated with genistein independent of pretreatment with cAMP signalling agonists. Interestingly, rescued ΔF508 CFTR in HeLa cells could be efficiently stimulated with either forskolin or genistein to promote Cl– transport. These results indicate that ΔF508 CFTR, when rescued in CFBE41o– human airway epithelial cells, is poorly responsive to signalling pathways known to regulate wild‐type CFTR. Furthermore, the differences in rescue and activation of ΔF508 CFTR in the two cell lines suggest that cell‐type specific differences in ΔF508 CFTR processing are likely to complicate efforts to identify potentiators and/or correctors of the ΔF508 defect.
Time-Varying Incentives in the Mutual Fund Industry

This paper re-examines the incentives of mutual fund managers arising from investor flows. We provide evidence that the convexity of the flow-performance relationship varies with economic activity. We show that the effect is economically large and is not driven by abnormal years. We test two possible channels through which this pattern may arise. We investigate implications of the time-varying convexity for the incentives of managers to alter strategically the risk of their portfolios. We provide evidence that poor mid-year performers increase the risk of the portfolio only when economic activity is strong. Finally, we briefly discuss some methodological implications.
Sorption and Solubility Characteristics of Compomer, Conventional and Resin Modified Glass-Ionomer Immersed In Various Media

Tooth coloured adhesive restorative materials are widely accepted and used for the restoration of carious and non-carious cervical lesions. The aim of this study was to evaluate and compare the solubility and sorption values of compomer (Dyract ®), conventional glass-ionomer(Fuji II ) and resin modified glass- ionomer (Fuji II LC improved) cements in various beverages such as tea, coffee, coca-cola and lime with saline as control. Twenty five circular discs per restorative material were prepared. Five specimens of each material were kept immersed in the test media and saline for seven days. Water sorption and solubility were calculated using ISO guidelines. Statistical analysis revealed statistically significant difference (P<0.05) between the three test materials. Among the media tested lime influenced both sorption and solubility values more than the other media. Fuji II was found to have more sorption and solubility values than the other two materials.
Stakeholder analysis of a centralized biogas plant in China — The case of Yangling

The paper conducted a stakeholder analysis for establishing a centralized biogas plant in Yangling, Shanxi province, China. Stakeholder positions in material flow system and their inter-relationships in the project were reviewed. Their interests and concerns of the project were further analyzed. The results revealed that lack of coordination among the indirect stakeholders, who enforced environmental protection regulations, regulated the subsidy policy and the requirement of connecting electricity to grid, were major challenges in developing the centralized biogas plant in Yangling.
Body Temperature Variation in Free-Ranging Hognose Snakes (Heterodon platirhinos)

Abstract Body temperatures (Tb) of eight free-ranging Heterodon platirhinos were measured with radiotelemetry throughout the year. Body temperature ranged from 5–37°C and varied with air temperature and activity. During the active season, Tb of active snakes (∼30.5°C) averaged about 3°C higher than that of inactive snakes (∼27.4°C). Body temperature did not differ between the sexes or between resident and nonresident snakes. At air temperatures below 30°C, Tb averaged ∼3°C above air temperature; at air temperatures above 30°C, Tb averaged ∼0.2°C below air temperature. Basking appears to be important in the thermal ecology of H. platirhinos. Body temperature of free-ranging active H. platirhinos monitored with radiotelemetry was similar to previous reports on Tbs of active H. platirhinos measured with cloacal thermometers. A thorough knowledge of basic thermal requirements of H. platirhinos may be useful in its conservation.
Transfer of IFNγ‐depleted CD4+ T cells together with CD8+ T cells leads to rejection of murine kidney sarcoma in mice

In the murine kidney sarcoma, vaccination with the tumor‐specific large T antigen induces protective immunity against the tumor. Immunity is dependent both on CD8+ cytotoxic T cells and on CD4+ T‐helper cells. We analyzed whether the cytokine phenotype of induced CD4+ T‐effector cells might determine whether or not the tumor is successfully rejected. By intracytoplasmic staining of CD4+ cells, IFNγ‐producing (Th1), IL‐4–producing (Th2), and IL‐10–expressing cells could be identified in vaccinated and non‐vaccinated animals responding to tumor growth. Vaccinated mice rejecting the tumor showed an increase in the percentage of IL‐4–producing (Th2) cells. In contrast, in non‐vaccinated mice succumbing to the tumor, the immunosuppressive IL‐10–producing cells became more abundant and the frequency of IFNγ‐expressing cells dropped at later time points. Yet, dominance by either a Th1 or a Th2 response could not be observed. To further clarify the relevance of these subsets, Th1 cells were enriched by cell sorting according to IFNγ surface expression. Enriched Th1 and depleted cells, mainly consisting of the Th2 phenotype, were transferred together with CD8+ T cells. Surprisingly, immunity could be transferred either with Th1 or Th2 cells, but Th2 cells were slightly more efficient. This suggests that, at least in the effector phase, a Th1 phenotype is not crucial for the rejection. Our findings support the view that the Th1/Th2 dichotomy is not central in T‐cell–mediated tumor rejection. Int. J. Cancer 87:673–679, 2000. © 2000 Wiley‐Liss, Inc.
Relationship of Stimulus and Examinee Variables to Performance on Analogous Visual and Tactile Block Construction Tasks

Nonverbal/spatial tests are unavailable for persons with visual impairments, despite decades of documented need and developmental effort. Because past tactile analogs of block design (BD) tests have not been widely accepted, known BD test parameters were compared across visual and tactile designs to assess the applicability of the test across modalities. Contrary to expectations, edge-cueing of designs with no perceptual cohesiveness (PC) improved tactile and visual performance. The expected PC by cueing and field independence (FI) by PC interactions were found for visual, but not tactile, BD. Uncued tactile designs elicited more errors, tending to occur closer to the center of the designs. These data suggest that visual and tactile BD performance cannot be interpreted similarly. Differences may be due to to modality-specific demand for various encoding and recoding abilities. The standing model is expanded to account for cross-modality differences in BD performance by including both rotation and block segregation.
A novel 5G TDD cellular system proposal based on multipath division multiple access

Evolving from the 3G and the 4G communication systems, the 5G system demands both high system capacity and high data rate. A novel time division duplexing (TDD) cellular system based on multipath division multiple access (MDMA) with massive antennas in millimeter wave band is proposed in this paper. The system is built on multipath division multiple access which is a method to use massive antennas at BS along with the Rake receiver and the Pre-Rake transmitter to achieve a processing gain for suppressing multiple access interference. The system concept is demonstrated by computer simulations. In addition, the associated transceiver architecture and a TDD time slot structure are presented for practical system concerns. Moreover, it is shown through analysis that the total average data throughput equals 3.8 Gbps and the system can achieve a bandwidth efficiency of 19 bps/Hz/cell on 200 MHz transmission bandwidth.
The three-layered core model permittivity of polymer nano-composites in electrostatic field

Multi-layered core model of nanoparticle was supposed to have advantage in analyzing dielectric characteristic of polymer nano-composites by many researchers. However this model cannot be verified effectively from the view of mathematic. This paper focused on solving this problem. First the three-layered core physical model was built based on physical phenomenon and former research results, and then mathematical model was built by taking different densities into Maxwell Garnett Approximation (MGA). Permittivity of each layer was established by integrals mean value point of supposed three different functions in respective thickness. At last three-layered core mathematical model of diffusion system was built and the equivalent permittivity was calculated. The calculated results have a better coincidence with experimental result and all of this show that three-layer core model and arc-tangent function have a good description of character of interaction zone.
Efficient Extraction of High‐Quality Total RNA from Various Hop Tissues (Humulus lupulus L.)

Abstract The isolation of high‐quality RNA is a prerequisite for gene expression studies. RNA quality is of special relevance if PCR‐based strategies such as cDNA‐AFLP or RT‐PCR are followed. Our molecular investigations of hop (Humulus lupulus L.) focus on genes that determine the biosynthesis of prenylflavonoids with interesting biological activities which accumulate in the lupulin glands. However, optimized protocols for RNA extraction from hop cones are not available. In this study, the RNeasy midi kit protocol was modified to isolate high amounts of total RNA from fresh and freeze‐dried hop tissues, specifically leaves, female inflorescences, and lupulin‐enriched hop cone fractions. The main difficulties in obtaining high RNA yields were related to specific features of hop, including the abundance of secondary metabolites and their accumulation in the sticky lupulin glands. The introduction of a number of modifications into the RNeasy midi kit protocol resulted in RNA of high quality, as assessed by spectrophotometry and electrophoresis in agarose gels. The protocol developed is currently being used to produce cDNA‐AFLP fingerprints of hop cones and leaves for genetic screening. Furthermore, the method could provide RNA for further molecular studies, including Northern blot hybridizations and reverse transcription‐polymerase chain reactions.
Impact of Exercise-Induced Muscle Damage on Performance Test Outcomes in Elite Female Basketball Players

Abstract Doma, K, Leicht, A, Sinclair, W, Schumann, M, Damas, F, Burt, D, and Woods, C. Impact of exercise-induced muscle damage on performance test outcomes in elite female basketball players. J Strength Cond Res 32(6): 1731–1738, 2018—The purpose of this study was 2-fold: first, to examine the impact of exercise-induced muscle damage (EIMD) on physical fitness qualities after a basketball-specific training session; second, to determine the reproducibility of the sport-specific performance measures in elite female basketball players. Ten elite female basketball players (age 25.6 ± 4.5 years; height 1.8 ± 0.7 m; and body mass 76.7 ± 8.3 kg) undertook a 90-minute training session involving repeated jumping, sprinting, and game-simulated training. Indirect muscle damage markers (i.e., countermovement jump, delayed onset of muscle soreness [DOMS], and creatine kinase [CK]) and sport-specific performances (i.e., change-of-direction [COD] test and suicide test [ST]) were measured before and 24 hours after training. These measures were also collected 1 week after training to determine the reproducibility of the basketball-specific performance measures. A significant reduction in lower-body power (−3.5 ± 3.6%; p ⩽ 0.05), while a significant increase in DOMS (46.7 ± 26.3%; p ⩽ 0.05) and CK (57.6 ± 23.1%; p ⩽ 0.05) was observed 24 hours after exercise. The ST was also significantly increased (2.1 ± 1.8%; p ⩽ 0.05), although no difference was observed for COD (0.1 ± 2.0%; p > 0.05). The intraclass correlation coefficient and coefficient of variation for the COD and ST were 0.81 and 0.90, respectively, and 1.9 and 1.5%, respectively. In conclusion, appropriate recovery should be considered the day after basketball-specific training sessions in elite basketball players. Furthermore, this study showed the usability of performance measures to detect changes during periods of EIMD, with acceptable reproducibility and minimal measurement error.
Accurate modeling of SOI Multi-Gate FETs and their transient response to radiation

Detailed, physics-based three-dimensional (3D) technology computer-aided-design (TCAD) device model, coupled in mixed-mode with external load circuit and parasitics, enabled accurate simulation of single-event effects (SEEs) in nonplanar silicon-on-insulator (SOI) Multi-Gate Field Effect Transistors (MuGFETs) or FinFETs. We show the importance of correct device physics models, including mobility in different crystal planes of strained silicon - validated with experimental data - for correct computation of both steady-state and transient characteristics of FinFETs. Mixed-mode coupling of a realistic load circuit, including experimental parasitics, with the 3D TCAD device model, is critical to be able to compute single-event transient current waveforms and charge collection characteristics that reflect well experimental results.
Expression of epidermal growth factor (EGF) and its receptor in bovine endometrium throughout the luteal phase: effects of EGF on prostaglandin production in endometrial cells

Epidermal growth factor (EGF) is produced in bovine endometrium throughout the estrous cycle. However, little is known about the expression of the EGF receptor (EGFR) and the roles of EGF in bovine endometrium. To clarify whether EGF is involved in local regulation of bovine endometrial function, first we determined the EGF protein and the expression of EGFR mRNA in endometrial tissues throughout the luteal stages. EGF protein concentration was higher (P < 0.05) in the mid (days 8-12) luteal stage than in the other luteal stages. EGFR mRNA expression was higher (P < 0.05) in the mid and late (days 15-17) luteal stages than in the other luteal stages. To investigate the protein concentrations of EGF and EGFR mRNA expression in cultured bovine endometrial cells, epithelial and stromal cells were isolated between day 0 and day 4 postovulation from 22 uteri. Both EGF protein concentration and EGFR mRNA expression were higher (P < 0.05) in epithelial cells than in stromal cells. Then, to examine the possible role of EGF in the regulation of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2), cultured endometrial epithelial and stromal cells were exposed to EGF (0, 1, 10 and 100 nm) for 24 h. In epithelial cells, EGF (10 and/or 100 nm) increased (P < 0.05) PGF2α and PGE2 secretion, but in stromal cells EGF (100 nm) increased (P < 0.05) PGF2α, but not PGE2 secretion. These results indicate that 1) the highest amount of EGF is produced by bovine endometrium at the mid-luteal stage, 2) endometrial EGFR mRNA expressions are higher at mid and lateluteal stages than other stages, 3) EGF is expressed mainly by uterine epithelial cells and 4) EGF has the ability to increase PGE2 and PGF2α production in both epithelial and stromal cells and therefore may play a role in local regulation of uterine function.
Constellations of Maternal and Paternal Parenting Styles in Adolescence: Congruity and Well-Being

ABSTRACT The current study examined patterns of maternal and paternal parenting styles in adolescence and the variations in adolescent well-being as a function of parenting style combinations. Participants included 272 students in grades 9 and 11 from a public high school in a large city in the northeastern United States. Participants completed measures of perceived maternal and paternal parenting styles and indices of psychological well-being. Distinct patterns of maternal and paternal style constellations were established. The most common constellation found was the combination of a neglectful mother and a neglectful father. Participants with either both parents authoritative or only mother authoritative reported higher well-being than participants with no authoritative parent. Participants with no permissive parent or with a permissive mother scored lower on self-esteem than participants with only a permissive father. Finally, participants with either parent neglectful or both parents neglectful scored lower on self-esteem than participants without a neglectful parent. The current study illustrates the need to examine constellations of maternal and paternal parenting in studies assessing the familial variables contributing to adolescent well-being.
Integral structures in the $p$-adic holomorphic discrete series

For a local non-Archimedean field K we construct GLd+1(K)-equivariant coherent sheaves VOK on the formal OK -scheme X underlying the symmetric space X over K of dimension d. These VOK are OK -lattices in (the sheaf version of) the holomorphic discrete series representations (in K-vector spaces) of GLd+1(K) as defined by P. Schneider [14]. We prove that the cohomology H(X,VOK ) vanishes for t > 0, for VOK in a certain subclass. The proof is related to the other main topic of this paper: over a finite field k, the study of the cohomology of vector bundles on the natural normal crossings compactification Y of the Deligne-Lusztig variety Y 0 for GLd+1/k (so Y 0 is the open subscheme of Pk obtained by deleting all its k-rational hyperplanes).
Development of Institutional Guidelines for Management of Gram-Negative Bloodstream Infections: Incorporating Local Evidence

Background: Appropriate empirical antimicrobial therapy is associated with improved outcomes of patients with Gram-negative bloodstream infections (BSI). Objective: Development of evidence-based institutional management guidelines for empirical antimicrobial therapy of Gram-negative BSI. Methods: Hospitalized adults with Gram-negative BSI in 2011-2012 at Palmetto Health hospitals in Columbia, SC, USA, were identified. Logistic regression was used to examine the association between site of infection acquisition and BSI due to Pseudomonas aeruginosa or chromosomally mediated AmpC-producing Enterobacteriaceae (CAE). Antimicrobial susceptibility rates of bloodstream isolates were stratified by site of acquisition and acute severity of illness. Retained antimicrobial regimens had predefined susceptibility rates ≥90% for noncritically ill and ≥95% for critically ill patients. Results: Among 390 patients, health care–associated (odds ratio [OR]: 3.0, 95% confidence interval [CI]: 1.5-6.3] and hospital-acquired sites of acquisition (OR: 3.7, 95% CI: 1.6-8.4) were identified as risk factors for BSI due to P aeruginosa or CAE, compared with community-acquired BSI (referent). Based on stratified bloodstream antibiogram, ceftriaxone met predefined susceptibility criteria for community-acquired BSI in noncritically ill patients (95%). Cefepime and piperacillin-tazobactam monotherapy achieved predefined susceptibility criteria in noncritically ill (95% both) and critically ill patients with health care–associated and hospital-acquired BSI (96% and 97%, respectively) and critically ill patients with community-acquired BSI (100% both). Conclusions: Incorporation of site of acquisition, local antimicrobial susceptibility rates, and acute severity of illness into institutional guidelines provides objective evidence-based approach for optimizing empirical antimicrobial therapy for Gram-negative BSI. The suggested methodology provides a framework for guideline development in other institutions.
Estimates of direct, maternal and grandmaternal genetic effects for growth traits in Gobra cattle

Foram obtidas as estimativas dos pesos ao nascimento (N = 3909), ao desmame (N = 3425), com 1 ano de idade (N = 2764) e final (N = 2144) a partir dos registros de gado Gobra coletados no Centro de Pesquisas em Zootecnia de Dahra, Senegal. Tres modelos animais foram adaptados para obter estimativas por REML usando uma abordagem de informacao media (AI). O modelo 1 considerou os efeitos ambientais aleatorios direto, genetico maternal e maternal permanente. No modelo 2, um efeito geral relativo as avos foi adicionado aos efeitos aleatorios considerados no modelo 1, e no modelo 3 o efeito geral relativo as avos foi dividido em efeitos ambientais genetico e permanente. Todos os modelos admitiram covariâncias entre os efeitos geneticos. A inclusao dos efeitos relativos as avos nos modelos 2 e 3 nao alterou as estimativas dos parâmetros geneticos comparados com o modelo 1. As variâncias atribuiveis aos efeitos relativos as avos tornaram-se negativas e foram posicionadas proximas a zero, exceto para o peso com 1 ano, para o qual a herdabilidade relativa a avo foi 0,03 ± 0,03. As estimativas para as herdabilidades direta e maternal foram, respectivamente, 0,08 ± 0,03 e 0,03 ± 0,02 para peso ao nascimento, 0,20 ± 0,05 e 0,21 ± 0,05 para peso ao desmame, 0,26 ± 0,07 e 0,16 ± 0,07 para peso com 1 ano e 0,14 ± 0,06 e 0,16 ± 0,06 para o peso final. As estimativas da correlacao genetica entre os efeitos direto e maternal para os pesos ao nascimento, ao desmame, com 1 ano e final foram -0,17 ± 0,40, -0,58 ± 0,32, -0,52 ± 0,34 e -0,34 ± 0,37, respectivamente. Para o peso com 1 ano com herdabilidade relativa a avo estimada como sendo apenas 0,03, o modelo 3 deu estimativas da correlacao genetica entre os efeitos direto e relativo a avo e entre os efeitos maternal e relativo a avo de 0,28 ± 0,48 e -0,33 ± 0,67, respectivamente. As estimativas de herdabilidade direta e maternal nao se alteraram quando os efeitos relativos a avo nao foram incluidos no modelo.
Fault Diagnosis of Analog Circuit Based on Wavelet Neural Networks and Chaos Differential Evolution Algorithm

A novel method for fault diagnosis of analog circuit based on chaos differential evolution wavelet neural networks (CDE-WNN) is proposed in this paper. In order to simplify network architectures and improve its learning accuracy and convergence rate, the architectures and parameters of wavelet neural networks are optimized by chaos differential evolution algorithm in the method. The fault dictionary is constructed in the weights of neural networks. The optimized WNN has the capability to detect and identify fault components in an analog electronic circuit. The simulation results show that the proposed method has not only the capability to reduce the effect on correct fault diagnosis due to components tolerance but also a small quantity of examples before test, fast diagnosis rate, and satisfactory accuracy of the diagnosis detection and location. A comparison of our work with WNN and BP algorithms, which reveals that our system requires a much smaller network and performs significantly better in fault diagnosis of analog circuits.