Miracle-dz/UniFAR-FTUs huggingface dataset:
DYE EXTRACTION METHOD EVALUATION OF THE SEALING ABILITY OF THREE TYPES OF ENDODONTIC SEALERS

This study aimed to: evaluate the sealing ability of AH Plus sealer, IRoot SP sealer, and Epiphany-Resilon root canal filling system by Dye extraction method. Methods: 45 freshly extracted single rooted human teeth were instrumented with Revo-S; the sequence used was SC1, SC2, SU, AS30, AS35, and AS40 taper 0.06. The prepared specimens were then divided randomly into Negative controls (n = 9) and Positive controls (n = 9); the negative controls were completely covered with three layers of varnish, and the positive controls were enlarged, but not root filled. The remaining 27 teeth were then randomly divided into three groups of 9 teeth each according to the type of sealer used, as the following: Group 1 (AH Plus sealer), Group 2 (IRoot SP sealer), and Group 3 (Epiphany-Resilon root canal filling system). Apical leakage evaluation was done by dye extraction methods. Data were statistically analyzed. Results: The positive control showed total dye absorbance of 5000, while negative control samples had a low absorbance of 75. The mean absorbance values were 370, 315, and 400 for Group 1 (AH Plus), Group 2 (IRoot SP), and Group3 (Epiphany-Resilon root canal filling system) respectively. Conclusion: The three tested sealers had the similar capability in apical sealing.
Rapid Identification of Streptoccus suis and Streptococcus equi subs.Zooepidemicus Based on 16S-23S rDNA Intergenic Spacer Region

Swine Streptococcicosis is an important pathogen of zoonosis mainly caused by Streptoccus suis(S.su-is)orStreptococcus equi subs.zooepidemicus(S.zooepidemicus)and generates grave threat to human health andpig-breeding.We developed a new rapid molecular method of identifying S.suisand S.zby PCR using onepair of primer derived fromthe highly conserved flanking region of the 3’end of16S and the 5’end of23S rD-NA genes.The size of PCR products showed polymorphism that the lengh of 21S.suisstrains were all 1 100bp,8S.zooepidemicusstrains were 1 300 bp,and no results in other strains and negative control.The PCRmethod,based on 16S-23S rDNAintergenic spacer region(ISR) was successful in rapid identifying these twopathogen species of Swine Streptococcicosis.The sequencing results of the PCR products of the two strains,HA9801 and ATCC35246,revealed thatthe base delection in some regions of16S-23S rDNAISR induced thevariance between these two species.
Isolation of the biofilm producing bacteria from osteomyelitis patients at Hospital Tengku Ampuan Afzan (HTAA), Kuantan

Aims: Accurate diagnosis and proper treatments of osteomyelitis are often difficult and ineffective due to several reasons such as less sensitive sample collected and the formation of biofilm following prosthetic use. Thus, our goal of this study is to identify suitable sample for laboratory diagnosis and also microbial species that cause osteomyelitic infection and discriminate between biofilm and non-biofilm producing strains in patients at Hospital Tengku Ampuan Afzan, Kuantan. Methodology and results: Samples of bone, prosthetic material, tissue and swab were collected from patient with suspected osteomyelitis at the hospital. Bacteria were isolated from sample using methods such as homogenization, direct transfer, and sonication. Then, species identification was done by colony characterization, biochemical test and the API identification system. Once species identified, tissue culture plate method was performed to discriminate the biofilm-producing strain from the non-biofilm-producing strain. The total number of 57 samples were collected from 17 cases of suspected osteomyelitis with 34 samples were found positive bacterial growth. Prosthetic samples produced highest positive growth with 81.3%, following by bone samples with 66.7% while swab and tissue samples with 46.2% and 43.8% respectively. We found that 14 from total 16 pathogens identified were biofilm producing-strains. Conclusion, significance, and impact of study: Prosthetic and bone samples produced higher bacterial growth, in contrast to other type of samples. Sonication method improves bacterial detection. Biofilm producing-bacteria were also the most common isolated strains from osteomyelitic infection. These have underscored the need to revise current clinical and laboratory practice as proper identification biofilm bacteria may influences management an outcome.
A Review and Construction of a Real-time Facial Recognition System

The evolution of surveillance technologies allows a reduction in human interaction with the process, since most of the monitoring functions performed by an individual can be replaced by detection and recognition techniques in real-time. This paper proposes the development of a surveillance system, which uses these techniques to identify individuals present within the field of view of camera. A combination of the Histogram of Oriented Gradient and Support Vector Machine techniques is applied for face detection, while a Residual Network is used during the stage of recognizing individuals. This shows the possibility of implementing this set of techniques, even in hardware with processing limitations.
Comparison in Chemical Compositions of the Essential Oil from Leaves of Lippia citriodora H.B.K. Plantlets Produced from Micro - propagation and Stem Cutting

Background: Lemon verbena (Lippia citriodora H.B.K.) is an aromatic and medicinal plant of family Verbenaceae, which cultivated in the North region of Iran. Objective: This comparative study was carried out on essential oil content and composition of Lippia citriodora H.B. K. from micro-propagated and rooted apical stem cutting plantlets. Methods: The micro-propagated plantlets were obtained by use of synthetic medium condition containing basal MS medium supplemented with 1 mg.l -1 BAP, 0.5 mg.l -1 IBA, 1 g.l -1 activated charcoal, 30 g.l -1 sucrose, and 7 g.l -1 agars. Pre-acclimated plantlets were transferred to the research greenhouse. To determine the essential oil content and its components in two types of plantlets, leaves of L. citriodora were collected in August 2013 before flowering stage. The essential oils were extracted by Clevenger apparatus and their constituents were determined by GC-MS. Results: The results indicated that thirty-two components were determined in the stem cutting derived plantlets. The main components of stem cutting plantlets were geranial (13.81%), limonene (11.41%), spathulenol (11.22%), and neral (11.21%). Thirty-five components were also obtained in the micro-propagated plantlets. The main components of micro-propagated plantlets were consisted of geranial (17.26%), neral (14.48%), 6-methyl-5-hepten-2-one (10.75%), and limonene (8.93%). Conclusion: In general, the essential oil content of stem cutting plantlets was higher than micro-propagated plantlets, while the number of compounds and amount of geranial and neral in stem cutting plantlets was lower than micro-propagated plantlets.
MOLECULAR PHYLOGENETIC RELATIONSHIPS OF SOME SPECIES OF NASSARIUS(GASTROPODA,PROSOBRANCHIA,NEOGASRTOPODA,NASSARIIDAE)BASED ON 16SrDNA FRAGMENT SEQUENCE

16SrDNA fragment sequence of 6 species of Nassarius were amplified and sequenced.Combing these with the homologous sequences of 2 species from GenBank and using Buccinulum line and Fusinus akitai as the out groups,the molecular phylogenetic trees of these groups were reconstructed with NJ and ME methods. The two molecular phylogenetic trees showed that 16SrDNA well resolve the genentic relationships at levels of species and subgenus of Nassarius. The genentic relationship between subgenus Reticunassa and Varicinassa was closer than that between subgenus Reticunassa and Zeuxis.The view that Nassarius (Zeuxis) semiplicatoides Zhang You,2007 is an independent species was also supported.
Fruit and seed development in two Hakea species (Proteaceae)

Hakea erinacea and H. trifurcata are related taxa that release their annual seed crop within a year of fruit maturity (weak serotiny). Both species produce similar-sized woody fruits (follicles) that protect two winged seeds. Fruit and seed development was completed 180 days (H. erinacea) and 215 days (H. trifurcata) after anthesis, with the fruits of both species reaching their maximum fresh mass during their ‘green’, non-woody state. This was associated with the commencement of wood formation in their fruit walls and the redistribution of certain nutrients (particularly phosphorus) from the fruit to developing seeds. Both H. erinacea and H. trifurcata retain chlorophyll in the walls of developing fruit, with H. trifurcata retaining chlorophyll in the mature fruit. This may have antiherbivore properties whereby the developing green fruits are cryptically disguised within a background of similarly green leaves.
Prediction of the carbon pool for lacebark pine under future temperature changes

The correlation between tree-ring widths of lacebark pine and climate was investigated. Radial growth was positively correlated with monthly mean temperatures in the previous September and significantly negatively correlated with monthly mean and monthly maximum temperatures in March. There was no significant response to levels of precipitation. Treering variation was reconstructed by a transfer function using STD chronology and monthly mean temperatures. Assuming future temperature increases of 1.4 °C to 4.0 °C according to IPCC (2006), tree-ring widths would increase by 4.7% to 12.8%, and lacebark pine carbon pools would increase observably by 10.9% to 31.1%. Temperature has a positive impact on increasing biomass and hence carbon stored for lacebark pine. The results provide a theoretical reference for forest management and an evaluation of the capacity for forests to sequester carbon under climate change.
Functional analysis of sabertooth cranial morphology

Elongate canines evolved independently at least four times among mammalian carnivores, and each time skulls were modified in similar ways. We have compared the cranial morphology of sabertooths to that of their non-sabertoothed relatives, living and extinct, and applied simple biomechanical models to elucidate the functional significance of the morphological differences. Our analysis suggests that (1) sabertooth morphology represents modification for wider gape with retention of a powerful bite force at the carnassial; (2) sabertooths probably used a throat or ventral neck slash to kill prey; and (3) elongate canines and retractile claws may have facilitated the exploitation of relatively larger prey by sabertooths compared to non-sabertooth carnivores.
Ecosystem Dynamics of the Boreal Forest: The Kluane Project

This book describes the Kluane Boreal Forest Ecosystem Project which operated from 1986 to 1996 in the southwestern Yukon. It begins by describing the area and its physical setting, and then the background of the project and the wisdom that had accumulated to 1986, on how this system might operate. The details of the experiments set up are presented, partly to help the reader appreciate the difficulty of working at -40 degrees and partly to aid the reader should they contemplate doing similar experiements in the future. Then they examine the three trophic levels of plants, the herbivores, and the predators in detail to provide some surprises about how the individual species operate within the overall system. Finally, they synthesize their findings in a model of the boreal forest vertebrate community, and provide an overview of what they have discovered and what remains to be done. Over the ten years of this project a large number of students and researchers have joined together to produce a picture which makes major advances in our understanding of the boreal forest ecosystem.
Dealing with farmers’ Ethnolinguistic differences when collecting crop diversity on-farm

Abstract Identification and characterization of the farmers’ named crop varieties cultivated around the world is a major issue for conservation and sustainable use of plant genetic resources. Intraspecific diversity is strongly determined by farmers’ socio-cultural environment, but this has little been documented. In this paper, we tested, on a contact zone among three ethnolinguistic groups located on the Mount Kenya region, whether farmers’ socio-cultural differences have an impact on the morphological characteristics of the farmers’ named sorghum varieties. Eighteen qualitative morphological traits of the panicles were measured. We first compared the morphological diversity of the named varieties among ethnolinguistic groups using multivariate analysis of homogeneity of groups’ dispersion and tested their differentiation using permutational multivariate analysis of variance. Discriminant analysis of principal components was then used to categorize the morphological diversity without a priori, and this classification was compared with farmers’ local taxonomy (vernacular names) in the three ethnolinguistic groups. Our results show that some morphotypes are peculiar to some ethnolinguistic groups and that a morphotype can bear different variety names while the same variety name can be used to identify different morphotypes. Morphological differentiation that was explained by ethnolinguistic groups was higher for local landraces than for improved varieties. Our findings imply that socio-cultural diversity of farmers and the criteria they use to identify and maintain landraces need to be considered in studying and sampling crop diversity for in situ as well as for ex situ conservation.
RAL-TR-97-040 Checking for Model Consistency in Optimal Fingerprinting

Current approaches to the detection of climate change and attribution of an anthropogenic influence on climate involve quantifying the level of agreement between one or more model-predicted patterns of anthropogenically-forced change and observed changes in the recent climate record. Analyses of uncertainty rely on simulated climate variability from a control run of a climate model. If the model gives an inaccurate representation of climate variability in the real world, all estimates of uncertainty, including statements of confidence in claims of attribution, are compromised. Any numerical representation of the climate system is likely to display too little variance on small spatial scales so there will always be aspects of model variability which are unrealistic, leading to a risk of spurious detection results. The risk is particularly severe i f the detection strategy involves optimisation of signal-to-noise because unrealistic aspects of model variability may automatically be given high weight through the optimisation procedure. The solution is to confine attention to aspects of the model and of the real climate system in which the model simulation of internal climate variability is adequate or, more accurately, cannot be shown to be inadequate. We propose a simple consistency check based on standard linear regression which can be applied to both the space-time and frequency-domain approaches to optimal detection and demonstrate the application of this check to the problem of detection and attribution of anthropogenic signals in the radiosonde-based record of recent trends in atmospheric vertical temperature structure. We also suggest results should be reported in terms of return-times in place of the usual confidence intervals, return-times being more transparent and less dependent on the distribution of climate noise.
Mayor histocompatibility complex class II (HLA-DR) is associated with morphea and systemic sclerosis patients

Morphea is a disorder limited to the skin, characterized by a stable oval plaque with a glossy plane surface that feels indurated on palpation. In contrast, systemic sclerosis is additionally characterized by disseminate cutaneous engrossment, sclerodactyly, the presence of Raynaud ´ s phenomenon, and internal organ involvement. Human leukocyte antigen (HLA)-DR4 class Ⅱ alleles are associated with morphea in Caucasians, whereas, HLA-DR4 presents as high frequency in Amerindians, besides it was associated with autoimmune disease. The aim of this study was to determine HLA-DR alleles in Mexican patients with morphea. This study recruited 24 morphea patients, whose HLA alleles frequencies were compared with HLA alleles frequencies presented in 22 systemic sclerosis patients and 99 ethnically matched healthy controls. The HLA-DR β 1 locus was genotyped based on the hybridization technique. HLA-DR4 and DR8 frequencies showed increases in morphea patients compared with healthy controls, whereas HLA-DR4 exhibited a statistical association with morphea when allele frequencies were compared with systemic sclerosis patients. Thus, HLA-DR β 1 associations varied in morphea and systemic sclerosis, suggesting the participation of different immunological molecular mechanisms.
Decreased Expression of Peroxisome Proliferator-activated Receptor α Gene as an Indicator of Metabolic Disorders in Stunting Toddler

BACKGROUND: Stunting in children increases the risk of degenerative diseases in adulthood, including dyslipidemia, obesity, type 2 diabetes mellitus, and cardiovascular disease. This is based on the result of metabolic changes that may be caused by chronic malnutrition and experienced by stunting children. Stunting in children is associated with metabolic disorders that are based on impaired fat oxidation, a trigger factor for obesity in adulthood. The peroxisome proliferator-activated receptor (PPAR) α gene is a transcriptional factor that regulates fat, carbohydrate, and amino acid metabolism whose genetic variants are linked to the development of dyslipidemia and cardiovascular disease. AIM: The study assessed the effect of metabolic changes in stunting toddler on PPARα gene expression. MATERIALS AND METHODS: An analytical-observational laboratory was done using 41 blood samples, coming from 23 stunting toddlers, and 18 not-stunting toddlers. In all research subjects, anthropometric measurements and examination of PPARα gene mRNA expression were carried out. Analysis of PPARα gene mRNA expression using one-step quantitative reverse transcriptase-polymerase chain reaction using specific primers, as a comparison of gene expression using the GAPDH gene. The relative expression of the PPARα mRNA gene was analyzed using the LIVAK formula. RESULTS: The study obtained a mean of ΔCT in stunting toddlers of 5.81, whereas in stunting toddlers at 5.082. Analysis with LIVAK 2 ^ - formula (ΔCT stunting -ΔCT not stunting) obtained PPARα mRNA gene expression of 0.6. CONCLUSION: We conclude that there is a decrease in PPARα gene expression in stunting toddlers.
The function of PAB1 in translation and in PUF3 dependent deadenylation

THE FUNCTION OF PAB1 IN TRANSLATION AND IN PUF3 DEPENDENT DEADENYLATION by Darren J. Lee University of New Hampshire, December 2007 The involvement of the poly(A)-binding protein (PAB1) in deadenylation and translation is well known. How PAB1 inhibits deadenylation and promotes translation is not well understood. I have analyzed PAB1 variants, containing entire domain deletions and substitutions of yeast residues with human residues. Chapter I discusses and provides in vivo translation rates of strains containing PAB1 variants, defects in mRNA degradation proteins, and defects in translation components. In chapter II, I address the role of PAB1 in regulated deadenylation. For this analysis I studied the effect of PUF3, a member of the PUF family of proteins that bind specific 3 ’ UTR sequences and accelerate deadenylation and/or repress translation of the target transcript. The analysis of in vivo translation rates, showed that while PAB1 did not have a major role in translation, its RRM1 and RRM2 domains were the most important for translation. I found defects in translation initiation factors, eIF4E and eIF3, had different effects on translation in combination with two PAB1 variants in the RRM2 domain that affect different aspects of translation in vitro. CAF1 and CCR4, two components of the CCR4-NOT complex responsible for deadenylation, have recently been shown to have separate functions in deadenylation. It has been suggested CAF1 and CCR4 have roles in
Vytautas Magnus University, Vileikos Str. 8, LT-44404, Kaunas, Lithuania UNSYMMETRIC DRY FRICTION FOR MODELS OF SURFACE CLEANING

1 Kaunas University of Technology, K. Donelaičio Str. 73, LT-44249, Kaunas, Lithuania 2 Kaunas University of Technology, Mechatronics Institute, Studentų Str. 56, LT-51424, Kaunas, Lithuania 3 Vytautas Magnus University, Studentų Str. 11, LT-53361, Akademija, Kaunas District, Lithuania 4 Company “Vaivora”, Palemono Str. 2a, LT-52191, Kaunas, Lithuania 5 Vilnius Gediminas Technical University, Saulėtekio Ave. 11, LT-10223, Vilnius, Lithuania 6 Vytautas Magnus University, Vileikos Str. 8, LT-44404, Kaunas, Lithuania
Assessment of endophytic fungi cultural filtrate on soybean seed germination

Soybean seeds have high amount of isoflavones but its germination is often confronted with a variety of environmental problems resulting in low germination rate and growth. To overcome this in eco-friendly manner, we investigated the influence of cultural filtrate (CF) of gibberellins-producing endophytic fungi on soybean seed germination. Three endophytic fungi namely: Chrysosporium pseudomerdarium , Aspergillus fumigatus and Paecilomyces sp. were previously isolated from the roots of soybean plants. The culture filtrate application of the three endophyte resulted in significantly higher rate of soybean seed germination, germination percentage, relative seed germination percentage, peak value, germination value, shoot and root length, germination index and vigour index. Among the endophytes, A. fumigatus significantly increased the rate of germination, shoot and root length and vigour index. Same trend was noted in germination percentage and relative seed germination percentage for all the endophytic fungi. However, C. pseudomerdarium was the only one that enhanced germination index. The enhanced soybean seed germination by endophytes can be used for seed priming and hence improved crop plant growth under extreme environmental conditions. Key words: Chrysosporium pseudomerdarium , Aspergillus fumigatus , Paecilomyces sp. , soybean, seed germination.
HISTONE H1 DEPLETION WITH HUMAN CELLULAR AGING: BIOCHEMICAL AND CYTOCHEMICAL EVIDENCES

Biochemical and immunocytochemical examinations of histone H1 in G1 arrested and senescent human fibroblasts were performed to pursuit the mechanisms of human cell aging in vitro. Examinations of histone synthesis in serum depleted or contact inhibited cell cultures indicated that the ratio of histone synthesis over DNA synthesis and molar synthesis of histone H1 to nucleosomal histones did not decline with accumulation of resting cell populations, while senescent cells had a lower ratio of histone H1 synthesis than young cells. Histone H1 contents plotted as a function of DNA synthesis activity revealed that senescent cultures had a lower content of histone H1 than young cultures at any stage of cell proliferation.Immunocytological study using antiserum against histone H1 on cocultivated young and senescent cells showed that immunofluorescence intensity was very low in senescent nuclei in comparison with young cell nuclei. Thus, we conclude that a decline in content and synthesis of histone H1 relative to nucleosomal histones with passage number was not simply due to the passage-related accumulation of resting cells, but actually reflects age-specific changes in cycling cells or whole cell populations in the senescent cultures. The depletion of histone H1 in chromatin was discussed as a possible cause of DNA strand break and relaxation of gene repression.