Miracle-dz/UniFAR-FTUs huggingface dataset:
A Simple Protocol to Compare EMFI Platforms

Several electromagnetic fault injection (EMFI) platforms have been developed these last years. They rely on diﬀerent technical solutions and ﬁgures of merit used in the related datasheets or publications are also diﬀerent. This renders diﬃcult the comparison of the various EMFI platforms and the choice of the one adapted to its own usage. This paper suggests a characterization protocol which application is fast and requires equipment usually available in labs involved in security characterization. It also introduces an eﬀective solution to enhance (by a factor 5) the timing resolution of EMFI platforms built around a commercial voltage pulse generator designed to drive 50 Ohm termination.
A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis

Within the Arabidopsis thaliana family of 21 heat stress transcription factors (Hsfs), HsfA9 is exclusively expressed in late stages of seed development. Here, we present evidence that developmental expression of HsfA9 is regulated by the seed-speciﬁc transcription factor ABSCISIC ACID–INSENSITIVE3 (ABI3). Intriguingly, ABI3 knockout lines lack detectable levels of HsfA9 transcript and protein, and further ectopic expression of ABI3 conferred the ability to accumulate HsfA9 in response to abscisic acid in transgenic plantlets. Consequently, the most abundant heat stress proteins (Hsps) in seeds (Hsp17.4-CI, Hsp17.7-CII, and Hsp101) were not detectable in the ABI3 knockout lines, but their expression could be detected in plants ectopically expressing HsfA9 in vegetative tissues. Furthermore, this seed-speciﬁc transcription factor cascade was reconstructed in transient b -glucuronidase reporter assays in mesophyll protoplasts by showing that ABI3 could activate the HsfA9 promoter, whereas HsfA9 in turn was shown to be a potent activator on the promoters of Hsp genes. Thus, our study establishes a genetic framework in which HsfA9 operates as a specialized Hsf for the developmental expression of Hsp genes during seed maturation.
Habitat preferences, foraging behaviour and bycatch risk among breeding sooty shearwaters Ardenna grisea in the Southwest Atlantic

Pelagic seabirds are important components of many marine ecosystems. The most abundant species are medium/small sized petrels (<1100 g), yet the sub-mesoscale (<10 km) distribution, habitat use and foraging behaviour of this group are not well understood. Sooty shearwaters Ardenna grisea are among the world’s most numerous pelagic seabirds. The majority inhabit the Pacific, where they have declined, partly due to bycatch and other anthropogenic impacts, but they are increasing in the Atlantic. To evaluate the sub-mesoscale habitat preferences (i.e. the disproportionality between habitat use and availability), diving behaviour and bycatch risk of Atlantic breeders, we tracked sooty shearwaters from the Falkland Islands during late incubation and early chick-rearing with GPS loggers (n = 20), geolocators (n = 10) and time-depth recorders (n = 10). These birds foraged exclusively in neritic and shelf-break waters, principally over the Burdwood Bank, ~350 km from their colony. Like New Zealand breeders, they dived mostly during daylight, especially at dawn and dusk, consistent with the exploitation of vertically migrating prey. However, Falkland birds made shorter foraging trips, shallower dives, and did not forage in oceanic waters. Their overlap with fisheries was low, and they foraged at shallower depths than those targeted by trawlers, the most frequent fishing vessels encountered, indicating that bycatch risk was low during late incubation/early chick-rearing. Although our results should be treated with caution, they indicate that Atlantic and Pacific sooty shearwaters may experience markedly differing pressures at sea. Comparative study between these populations, e.g. combining biologging and demography, is therefore warranted.
Microsoft Word-paper_slit_xfem.docx

This study presents a simple approach to model unidirectionally arrayed chopped strand (UACS) laminates via the extended finite-element method to represent slits (i.e., fiber cutting lines). This enables the introduction of slits independent of the finite-element mesh and reduces the effort to represent a complex discontinuity pattern when compared to standard modeling using double nodes. The laminated structure was represented by stacking two-dimensional Ac ce pt ed M an us cr ipt
Conceptual model of Ontology in Education Era

- The Ontology Problem is a fundamental challenge of the emerging Semantic Web. This problem is comprised of three key sub-problems that are higher level Ontology Problem, the domain ontology problem and the Ontology Integration Problem. Key issues are OWL (web based ontology) reasoning is limited and has performance issues, Multi-threaded access is unstable and some API’s are confusing. This paper derives current status of ontology in education domain also focusing on how to develop a conceptual and strategic model which helps us to develop an ontology in terms of Natural language parsing (NLP) , XML (extended markup language), OWL and RDF (resource descriptor framework).
Extremely Non-uniform: Patterns of Runs of Homozygosity in Bovine Populations

Runs of homozygosity (ROH) are regions of the genome that are contiguously homozygous because the parents of an individual have transmitted identical haplotypes. Long ROH indicate a recent common ancestor while very short ones are indicators of demographic history of a population. The distribution of ROH along the genome has been shown to be extremely non-uniform in human populations. We have analyzed patterns of runs of homozygosity from the Illumina BovineHD BeadChip, featuring more than 700,000 autosomal SNPs for the taurine cattle breeds Angus, Brown Swiss and Fleckvieh as well as for the indicine breeds Brahman, Gir und Nelore. ROH of lengths >1 Mb were accepted. The average proportion of individuals for which any SNP was in a ROH across the genome was . 081 (.069 for indicine breeds and .096 for taurine breeds), 99.0 and 99.9 percentiles were . 183 (.165, .241) and . 275 (.330, .406), respectively. ROH islands, i.e. regions with highest incidences (>.40) were partly overlapping and partly distinct between subspecies and breeds within subspecies. ROH islands were surprisingly gene rich compared to equally sized regions in distant regions of the same chromosomes. The genesis and function of ROH islands is still unclear and worth pursuing. Analysis of whole genome sequence data will add new challenges of analysis and prospects of better understanding the biology of ROH islands.
Simultaneous Detection of Actinobacillus pleuropneumoniae and Haemophilus parasuis in Pig by Duplex Droplet Digital PCR

Actinobacillus pleuropneumoniae ( A. pleuropneumoniae ) and Haemophilus parasuis ( H. parasuis ) are important pathogens of swine, which cause great economic losses in the swine industry and bring great safety hazards to food safety. In this study, we established a duplex Droplet Digital Polymerase Chain Reaction (ddPCR) assay for sensitive detection of A. pleuropneumoniae and H. parasuis simultaneously in pork. The limit of detection of plasmid DNA could reach 2 copies/μL for both A. pleuropneumoniae and H. parasuis ; which were approximately 50- and 20-fold greater sensitivity than Quantitative real-time PCR (qPCR). Both qPCR and ddPCR showed high linearity and positive correlation with standards. The detection results of clinical suspected samples for A. pleuropneumoniae and H. parasuis indicated the positive detection rate of ddPCR (10.7% and 12.5%) was higher than that of qPCR (7.1% and 8.9%). Therefore, the duplex ddPCR assay could be used as an effective quantitative detection to diagnose A. pleuropneumoniae and H. parasuis simultaneously, which may lay a foundation for the development of substitute rapid diagnostic technique.
Breeding for Resistance to Aphanomyces euteiches Root Rot and Rhizoctonia solani Stem Rot in Peas

A modified Environmental Shift Technique based on use of a disease index (scoring) gave consistent separation between susceptible and moderately resistant pea (Pisum sativum L.) genotypes to Aphanomyces euteiches Drech. root rot in 3 tests. A moderately high association (r = −0.63 to −0.83) between disease index and percentage of plant survival was found in segregating populations. Minnesota 108, moderately resistant to A. euteiches, produced adventitious roots readily at an early stage. Root rot resistance and number of adventitious roots were inherited independently. Broad sense heritability (BSH) ranging between 0.45 and 0.57 for resistance to A. euteiches root rot, and between 0.39 and 0.44 for resistance to Rhizoctonia solani Kuehn stem rot, varied by parental combination, experiment, and method of estimation. However, heritability based on gain by selection in the F3 ranged from 0.28 to 0.46 and from 0.21 to 0.44 for resistance to A. euteiches and R. solani, respectively. Frequency distribution of resistant and susceptible plants suggested quantitative inheritance of resistance to both diseases. Recurrent selection, in which each cycle includes one intermating, selfing, and testing generation, is suggested to improve and transfer resistance.
Genotypes and Biotypes Variation of Bovine Viral Diarrhea Virus from Persistently Infected Dairy Cattle in Java, Indonesia

The objective of this research was to study the genotype and biotype of BVDV variability among PI dairy cattle in Java, Indonesia between 2016 and 2017. Two hundred isolated buffy coat from dairy cattles that had low reproductive performance and never been vaccinated in Java were used in this studies. Using antigen capture Elisa, 12 out of 200 dairy cattles were positive for the presence of protein Erns BVDV (6.0%). The PI status was confirmed by multiple sequential viral molecular detection. Through phylogenetic and nucleotide sequence analysis of the 5’-Untranslated Region (5’UTR) of the samples investigated, it was determined that all the 12 field positive samples had the BVDV-1 genotype. Three IP-BVDV positive samples (2282-15, 061014 and 0813-2) sharing highest similarity (99% homology) with subgenotype BVDV-1c AY763030-1 and KF896608 isolates from Australia. Using Immuno Peroxidase Monolayer Assay (IPMA) the biotype of all the samples were identified as noncytopathic-BVDV. All of the informations would be necessary for designing the diagnostic tool and/or vaccine that match the circulating BVDV subgenotype.
Transforming Education with Emerging Technologies in Higher Education: A Systematic Literature Review

This study attempts to understand what is known about key theme findings in transforming education with emerging technologies in higher education by examining existing literature. Based on 5 selection criteria, 24 quality articles were included in the review. Thematic analysis methods are used to analyze and identify key themes in the data. The findings indicate that teachers who have used emerging technologies in teaching, they point out the key factors for transforming education with emerging technologies, including teachers' interest, institutional perspective, teachers' perceptions of the benefits of emerging technologies. They also report a dichotomy between the technologies used for teaching in higher education institutions and the technologies owned and used by students in social life as a major challenge. Teachers believe that the open communication and teamwork environment can be enhanced by using emerging technologies. Pedagogical innovation, empowering educators are essential requirements for teaching with emerging technologies. The 7 findings from this study should be used to guide initiatives for teacher career development to improve the effectiveness of education with emerging technologies.
Calcium-induced aggregation of dissolved and colloidal substances in mechanical pulp suspensions

On a etudie l'agregation, induite par le calcium, des substances dissoutes et colloidales provenant de suspensions de PTM faite d'epinette de Norvege. A des concentrations depassant les 10 mmol/L de CaCl 2 ajoute, les substances colloidales extraites de la PTM ecrue n'etaient que partiellement agregees, cependant que les substances colloidales prelevees dans la pâte PTM blanchie au peroxyde alcalin etaient completement agregees. L'agregation etait causee par les interactions entre le calcium et les acides polygalacturoniques. La depolymerisation des acides polygalacturoniques au moyen du traitement a la pectinase de la PTM blanchie n'a pas empeche l'agregation complete induite par le calcium de se produire dans les echantillons de pâte blanchie. Toutefois, ce meme traitement a reduit le taux d'agregation (.)
First evidence of the deep-sea fungus Oceanitis scuticella Kohlmeyer (Halosphaeriaceae, Ascomycota) from the Northern Hemisphere

Abstract We report on a collection of the deep-sea fungus Oceanitis scuticella from recently sunken wood at abyssal depth in the Northwest Pacific Ocean. The fungus was originally described as wood-associated from the Angola Basin. Subsequently, it was also found on sunken wood in the Southwest Pacific Ocean. Ascomata and ascospores of O. scuticella were examined by scanning electron microscopy and light microscopy. The phylogenetic affiliation of the fungus within the Halosphaeriaceae was demonstrated using partial nuclear internal transcribed spacer (ITS) regions and large subunit (LSU) rDNA sequencing. Slight morphological differences between this collection and previously described material were observed concerning ascomata shape, namely the drop-shaped cavity, the form of the hypostroma and the thickness of the peridium. In addition, the ascospores were smaller than those reported in previous collections. LSU rDNA phylogeny provided a good support for the placement of the NW Pacific Ocean sample within O. scuticella. The ITS rDNA sequence of the present collection differed from those of the earlier collections by 2.5–3%, a value accepted for intraspecific variation in fungi. Based on the present material, some factors interpreted as indicators for true deep-sea fungi, such as the absence of bark or co-existing xylophagic bivalves in sunken wood, are discussed and their importance is considered as limited.
Design of Learning Media: Modeling & Simulation of Building Thermal Comfort Optimization System in Building Physics Course

The use of instructional media is something that can support the teaching and learning process; therefore, a lecturer must have the ability to create and develop learning media. This study aims to improve student learning outcomes in building physics course by using simulation learning media and models to help students understand thermal comfort material. Making modelling and simulation media is done using MATLAB software. The subjects of this study were physics engineering students who took Building Physics course. At the beginning of the study, students are given material and then in groups discuss thermal contents and then given a pretest test with an average score of 70.27, and for an average grade of 71.3 assignments. At the meeting next week, using the Student-Centered Learning (SCL) method and using problem-based learning in groups, students take temperature measurements in several rooms in the Building at the UNAS Physical Engineering Laboratory. The measurement results show that the room does not have thermal requirements (PERGUB No. 38/2012), so students have the task of conducting experiments using models that have been created by researchers to create learning media to improve comfort in using thermal buildings. Simulation results carried out by students, that is, can produce rooms with thermal conditions at 21-25°C (PERGUB No. 38/2012). This simulation is also able to provide the score of building energy efficiency. After students succeed in conducting the test, the assessment test or posttest is carried out with an average score obtained 80.55, and an average score of 80 assignments. The results of the pretest, assignment 1, assignment 2, and posttest show an increase in students’ scores of 14.6% for the Test and Task Score of 12.20%. Based on the hypothesis test, for both variables showed t-count < t-table and significance < 0.05. It shows there are significant differences in student learning outcomes both test scores and assignment scores before and after using a simulated media. Thus, the system and simulation model designed can be used as learning media that can improve student learning outcomes.
DROSOPHILA MELANOGASTER IN RESPONSE TO TEMPERATURE

We examined the evolutionary and developmental responses of body size to temperature in Drosophila melanogaster, using replicated lines of flies that had been allowed to evolve for 5 yr at 25? C or at 1 6.5? C. Development and evolution at the lower temperature both resulted in higher thorax length and wing area. The evolutionary effect of temperature on wing area was entirely a consequence of an increase in cell area. The developmental response was mainly attributable to an increase in cell area, with a small effect on cell number in males. Given its similarity to the evolutionary response, the increase in body size and cell size resulting from development at low temperature may be a case of adaptive phenotypic plasticity. The pattern of plasticity did not evolve in response to temperature for any of the traits. The selective advantage of the evolutionary and developmental responses to temperature is obscure and remains a major challenge for future work.
Reactivity of Iminophosphoranes. Formation of the Piperazine Ring

Reaction of 3-azidopropyl methanesulfonate with triphenylphosphine produced the 1,4-bis(triphenyl-phosphino)piperazine 5 by intermolecular cyclization, instead of formation azidine derivatives by an intramolecular pathway. Structural formulation of 5 was achieved by both spectral and X-ray crystal analysis. Hydrolysis of 5 under basic condition gives piperazine, whereas reaction of lithium diphenylphosphide with 5 produced 1,2-bis(diphenyl-phosphino)ethane (dppe) exclusively.
INVESTIGATIONS INTO THE EFFECTS OF ALTERED TITIN ISOFORM EXPRESSION IN STRIATED MUSCLE

The giant protein titin is found primarily in striated muscle, where it is responsible for a number of roles. In order to modulate passive tension and provide structural support within the sarcomere, titin is subjected to multiple alternative splicing events and is present as several different isoforms. The extent of titin phosphorylation also controls resting tension in the myocardium. Recently, it was reported that Rbm20 is the splicing factor responsible for titin alternative splicing, and its deletion leads to the expression of a giant titin isoform. Homozygous mutant (Hm) rats lack a functional Rbm20, and the sarcomeric proteins’ isoform expression was compared to wild type (Wt) rats. Titin phosphorylation was also examined. We report no difference between titin phosphorylation in Wt and Hm rats, as the optical density vs. protein load was similar (0.13 vs. 0.17, respectively). Further, the cardiac myosin heavy chain (MHC) isoform expression was similar between Wt and Hm. Wt rats expressed 66.9% α-MHC and 33.1% β-MHC, and Hm expressed 60.4% α-MHC and 39.6% β-MHC. The MHC content of the tibialis anterior (TA) skeletal muscle was also studied, and the proportions of type IIX and type IIB MHC were similar across genotypes as well. TA sarcomeric proteins’ expression level was measured, and no differences in expression of actin, myosin light chain (MLC)-1, MLC-2, MLC-3, tropomyosin, troponin C, troponin I, or troponin T were 59 found to exist. Thus, it appears that the Hm rat mutation primarily shows differences to the titin protein, while the remaining sarcomeric proteins are unchanged. At present, the lack of a working Rbm20 gene only seems to affect titin alternative splicing.
Global Analysis of Genes Regulated by Low Temperature and Photoperiod in Peach Bark

In response to environmental cues plants undergo changes in gene expression that result in the up- or down-regulation of specific genes. To identify genes in peach Prunus persica (L.) Batsch. trees whose transcript levels are specifically affected by low temperature (LT) or short day photoperiod (SD), we have created suppression subtractive hybridization (SSH) libraries from bark tissues sampled from trees kept at 5 degrees C and 25 degrees C under short day (SD) photoperiod or exposed to a night break (NB) interruption during the dark period of the SD cycle to simulate a long day (LD) photoperiod. Sequences expressed in forward and reverse subtractions using various subtracted combinations of temperature and photoperiod treatments were cloned, sequenced, and identified by BLAST and ClustalW analysis. Low temperature treatment resulted in the up-regulation of a number of cold-responsive and stress-related genes and suppression of genes involved in "housekeeping" functions (e.g., cell division and photosynthesis). Some stress-related genes not observed to be up-regulated under LT were increased in response to SD photoperiod treatments. Comparison of the patterns of expression as a consequence of different temperature and photoperiod treatments allowed us to determine the qualitative contribution of each treatment to the regulation of specific genes.
In-vivo and In-vitro phytochemical GC-MS analysis of volatile constituents of Andrographis paniculata (Burm.f.) Nees

Aim: The present study aims at identifying and comparative analysis of various active phytochemicals from plant parts (leaf and stem) and in-vitro callus sample of Andrographis paniculata using Methanolic and Petroleum Ether Extracts through GC-MS technique. Methodology: Andrographis paniculata leaf, stem and callus (obtained from leaf explant) samples were dried at room temperature, powdered and then sequentially extracted in Methanol and Petroleum Ether as solvents using soxhlet apparatus. Total five extracts were prepared viz: Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MSE), Methanolic Callus Extract (MCE), Petroleum Ether Leaf Extract (PELE) and Petroleum Ether Stem Extract (PESE). Further, these five extracts obtained were analyzed for the presence of various volatile phytochemical compounds using GC-MS technique. Results: The GC–MS analysis of the five extracts revealed the presence of 34 different high and low molecular weight phytochemicals with varying quantities. Some of the phytochemicals detected in A. paniculata are 1-(+)-Ascorbic acid 2,6-dihexadeconoate, Eicosane, à-D-Glucose, 3-Nitrobenzyl iodide,2Pentadecanone 6,10,14-trimethyl, 6-Tetradecanesulfonic acid, butyl ester, Phytol,1-Hexyl-2nitrocyclohexane, N-'Methy-l-N-'phenyl-N-tosylformohydrazide etc. These phytochemicals are considered biologically active and pharmacologically important. Conclusion: The results obtained from the comparative GC-MS analysis of different samples of A. paniculata shows the presence of various bioactive compounds with some newly identified compounds. Therefore, A. paniculata is recommended as a phytopharamaceutically important plant.
Synthesis and Antiviral Activity of 9-alkoxypurines: New 9-(hydroxyalkoxy) Derivatives of Guanine and 8-methylguanine

A number of 9-(hydroxyalkoxy) derivatives of guanine (27a-h and 27l-n) and of 8-methylguanine (27i-k) have been synthesized and evaluated as antiviral agents against herpes viruses (HSV-1, HSV-2, VZV) and visna virus, a lentivirus. The synthesis Involved reaction of hydroxyl-protected derivatives of hydroxyalkoxy-amines (6a-m) with 4,6-dichloro-2,5-diformamidopy-rimidine (24) followed by cyclization of the resultant 6-alkoxyaminopyrimidines (25a-m) to either guanine derivatives (27a-h and 27l-n) or 8-methylguanine derivatives (27i-k). Modest antiviral activity was seen with 27b, 27c, 27f, 27i and 271 against the herpesvirus and with 27e and 27m against visna virus. Only 27e showed any cytotoxicity in MRC-5 cells at concentrations ≤400μM.