Miracle-dz/UniFAR-FTUs huggingface dataset:
LncRNA Profiling of Exosomes Derived From Staphylococcus Aureus-induced Bovine Mammary Epithelial Cell Lines MAC-T

Background: Exosomes (carrying proteins, miRNA, lncRNA, etc.) play a vital role in participating in the occurrence and development of mastitis. LncRNA can play a variety of regulatory roles by combining with protein, RNA, and DNA. The expression of mRNA and lncRNA in exosomes derived from bovine mammary epithelial cells infected by S. aureus is rarely understood.Methods: We used ultracentrifugation to separate exosomes derived from S. aureus- stimulated MAC-T cells. After extracting exosomal RNA, RNA sequencing was performed. We screened differentially expressed genes, mRNA and lncRNA, and predicted their functions through KEGG and GO analysis. 6 mRNA and lncRNA were randomly selected for RT-qPCR verification.Results: Analysis of the sequencing results showed that there were 186 differentially expressed genes, 431 differentially expressed mRNAs and 19 differentially expressed lncRNAs in the exosomes derived from S. aureus-infected and non-infected MAC-T acells. By predicting lncRNA target genes, it was found that 19 differentially expressed lncRNAs all acted on multiple mRNAs in cis and trans. GO analysis revealed that differentially expressed genes and lncRNA target genes played significant roles in such metabolism, transmembrane transport, cellular response to DNA damage stimulus, response to cytokines. KEGG enrichment indicated that lncRNA target genes gathered in the TNF pathway, Notch pathway, phosphatidylinositol signaling system and p53 pathway. Conclusion: The data suggested that S. aureus induced changes in genes, mRNA, and lncRNA in exosomes secreted by MAC-T cells, which furtherly triggered other cells damage through exosomal delivery. This study would provide valuable resource for understanding the lncRNA information in exosomes derived from dairy cow mammary epithelial cells, and conduced to the study of S. aureus infection in dairy cow mammary glands.
Ion transport in ionomeric polymers for ionic electroactive polymer devices

Recently, electroactive polymers (EAPs) have received immense attention and interest from the materials community because of their promising properties such as light weight, high elastic energy density and easy processing, which provide them the applicability in wide areas including solar cells, super capacitors, actuators and sensors. Among wide variety of electroactive polymers, ionic electroactive polymer (IEAP) has been proven more practical for both actuator and sensor applications. This dissertation discusses the the limiting factors in IEAP actuators and sensors. Three important components, ionomeric polymer membrane, conductive network composites (CNCs) and electrolytes, all have significant determination on the performance of IEAP actuators and sensors. Thorough investigation are conducted by both experimental and theoretical methods, and the findings are presented in this dissertation. We first investigated how the morphology of CNC thin-film influences the mechanoelectrical performance of IEAP sensors. IEAP sensor, in most cases, is also referred to as ionic polymermetal composite (IPMC) sensor. A novel approach, layer-by-layer (LbL) ionic self-assembly technique is utilized to fabricate the porous and conductive CNC nanocomposites. The electrochemical, morphological characteristics, and the corresponding mechanoelectrical performance of this IEAP sensor were explored as a function of the CNC morphology. Meanwhile, the influence of ionic liquids (ILs) concentration on the electromechanical response of IEAP actuators has been investigated. It was observed that an optimum concentration of ions where the electromechanical response is maximized is achieved by adjusting the uptake of IL in the ionomeric membrane; this optimum concentration, however, is not the highest ion concentration. Functional ionomeric polymer membrane is the backbone of a wide range of ionic devices due to its permeability to ions, which is the principle of these devices. Ions are sourced by
Direct and Indirect Therapeutic Effect of Traditional Chinese Medicine as an Additional Drug on Non-proliferative Diabetic Retinopathy: A systematic review and meta-analysis of high-quality studies

Bac﻿kground Diabetic retinopathy (DR) is the leading cause of blindness in many countries. The current treatment of non-proliferative DR (NPDR) with Western medicine (WM) alone are still insufficient. At present, the treatment of NPDR with the combination of traditional Chinese medicine (TCM) and WM is universally applied, and we would evaluate the effectiveness and safety of TCM as an additional drug for NPDR with systematic reviews and meta-analysis. Method Data before July 6, 2019 were searched as randomized controlled trials (RCTs) of TCM for the treatment of NPDR with WM, which were collected from China National Knowledge Infrastructure , Wanfang Database , China Biomedical Database , Pubmed , Embase and Cochrane Library . Relevant data was extracted by two reviewers respectively. I 2 statistics was adopted to appraise the heterogeneity, if I 2 <50% then the fixed-effects model would be employed, otherwise the random-effect model would be employed. (PROSPERO: CRD42019134947) Result 18 RCTs (1522 patients) were included according to the inclusion criteria. The results showed that compared with WM, TCM (including Compound Xueshuantong Capsule, Qiming Granule, and others)+ WM for NPDR could improve overall efficiency [n=1686,RR1.24(1.18,1.30), P <0.00001, I 2 =0%], and reduce the level of risk factors related to NPDR, such as glycated hemoglobin level [n=360, MD -0.85(-1.28, -0.41), P =0.0001, I 2 =72%], triglyceride and total cholesterol, but not Qiming Granule. Moreover, no serious adverse events were reported. Conclusion Compared with WM alone, TCM+WM could significantly improve NPDR, and also reduced the correlation levels of risk factors, considering the sample size and the number of patients included in the study, there might be publication bias, so the corresponding results should be treated with caution.
Experimental data and thermodynamic modelling of gallic acid solubility in a variety of pure solvents

ABSTRACT The solubility of gallic acid (GA) in butan-1-ol, butan-2-ol, hexan-1-ol, 1,4-dioxane, ethyl acetate, methyl acetate and cyclohexanone from 293.15 to 318.15 K has been experimentally measured using a thermostatted reactor and UV/vis spectrophotometer analysis. The results demonstrate that the solubility of GA generally increased with the temperature and at a given temperature decreased with the increase of the carbon number of alcohols. The van’t Hoff model, modified Apelblat equation, h equation and the Two Local composition models of Wilson and NRTL model were used to predict the reported solubilities, resulting in a mean relative deviation of 3.47%, 3.54%, 2.90%, 3.12% and 3.18%, respectively. The obtained data showed that the h equation model is the most suitable for description of the solid–liquid equilibrium containing GA. Furthermore, the standard molar Gibbs energy and standard molar enthalpy of dissolution of GA were estimated.
Briefing note : Does education raise people ’ s productivity or does it just signal their existing ability ?

Understanding the relative importance of these two mechanisms is important for governments’ education strategies. If education really does make individuals more productive then this will lead to higher economic growth, and many other benefits, meaning government should invest in it. On the other hand, if education is merely acting as a signal of pre-existing ability then it is less clear that government should spend large amounts of money educating populations.
ACTION-COLLABORATIVE NETWORKS OF THE REGIONAL GOVERNMENT ON LAND AND FOREST FIRE RESTRAINT IN PELALAWAN DISTRICT, RIAU PROVINCE

The problems of forest and land fires continue to overshadow in Riau Province and Pelalawan District including the contributors of fire spots of forest and land fires. The handling and control of forest and land fires run during this time tend to be incidental and focus on the aspect of fire suppression only. The extinguishing and controlling of forest and land fires fire has been undertaken by several agencies in the local government organization of Pelalawan District. While the relationship between the agencies is more information share and informational relationship and the deployment of resource assistance is limited to the capacity of each organization during the occurrence of forest and land fires only, not led to a collaborative multi-disciplinary working approach (inter-organizational collaborative network) in the area of local government. This research aims to Described the management situation of forest and land fires that took place in Pelalawan District, conducting analysis and academic studies on the practice of Network Inter-Organization by controlling forest and land fires (Karhutla) in Pelalawan District, and formulating model Inter-organizational network-based organizations that can be applied to regional government of Pelalawan District in every bureaucracy organizations. The study used a qualitative descriptive approach with reference to literature studies and interview reluct collection by (Agranoff i McGuire, 2003).
Genome-wide DNA Variants Identify Genetic Diversity and Population Structure of Leptosphaeria maculans in Western Canada

Background: Leptosphaeria maculans is a serious concern for canola production in Canada. For effective management, knowledge of the pathogen’s genetic variability and population structure is a prerequisite. Despite some information on race dynamics of the western Canadian L. maculans population in recent years, genetic diversity based on a large number of genome-wide DNA variants has not been investigated.Results: From 1,590 L. maculans isolates collected from 23 field sites in three provinces: Manitoba, Saskatchewan and Alberta, Canada, in the years 2007-2008 and 2012-2014, 150 representative isolates were selected and whole-genome sequenced, and 31,870 polymorphic DNA variants (SNPs and InDels) were used to study L. maculans genetic diversity and population structure. Cluster analysis showed that the genetic diversity levels and isolate groupings varied with the number and genomic regions of the variants involved; isolates collected in 2012-2014 were more genetically diverse than those collected in 2007-2008 when genome-wide variants were considered. The genome wide association study (GWAS) detected variants in egn4_Lema_T86290 (AvrLm4-7), egn4_Lema_T86300 and egn4_Lema_T86310 associated with the year of collection, but no variants was found to be associated with the province or specific location from which the isolates were collected. Population structure analysis indicated the presence of three distinct sub-populations in western Canada. While isolates from Saskatchewan were mainly of one sub-population (sub-pop1), the Alberta isolates comprised two sub-populations (sub-pop1 and sub-pop2), and all the 3 subpopulations were found in Manitoba.Conclusion: The genetic diversity of the western Canadian L. maculans population varied among provinces. It was highly admixed in Manitoba, followed by that in Alberta. The Saskatchewan population had the lowest genetic diversity. Significant genome variation between 2007-2008 and 2012-2014 occurred in the genes egn4_Lema_T86290 (AvrLm4-7), egn4_Lema_T86300 and egn4_Lema_T86310), with AvrLm4-7 becoming much more common in the L. maculans population in the later period.
Effects of complexant concentration in base solution on the structure and electrochemical properties of LiNi0.8Co0.1Mn0.1O2

LiNi0.8Co0. 1Mn0.1O2 powders were synthesized from co-precipitated spherical metal hydroxide, Ni0.8Mn0. 1Co0.1(OH)2. The effects of the complexant (NH4OH) concentration in base solution on the microstructure, crystalline structure and electrochemical properties of the product were studied. The results of scanning electron microscopy showed that the particle morphology was more regular and the particle size tended to increase with improving the concentration of NH4OH. The as-prepared material had a good α-NaFeO2 hexagonal lamellar structure, as confirmed by X-ray diffraction pattern, the sample had the least Li/Ni cation disorder when the concentration of NH4OH was 1.5M, which contributing to good cycle performance, its initial discharge capacity achieved 198.5 mAh g and the capacity retention rate was 95% after 50 cycles at 0.2C between 2.8 and
Impact of Robinia pseudoacacia stand conversion on soil bacterial communities composition and soil properties in Mount Tai, China

Background: Robinia pseudoacacia is a widely planted pioneer tree species in reforestations on barren mountains in northern China. Because of its nitrogen-fixing ability, it can play a positive role in soil and forest restoration. After clear-cutting of planted stands, R. pseudoacacia stands become coppice plantations. The impacts of shifting from seedling to coppice plantations on soil bacterial community and soil properties have not been well described. This study aims to quantify how soil properties and bacterial community composition vary between planted seedling versus coppice stands.Methods: Three 20×20 m plots were randomly selected in each seedling and coppice stand. The bulk soil and rhizosphere soil were sampled in the nine above-mentioned sample plots in the summer of 2017. Bulk soil was sampled at 10 cm from the soil surface using a soil auger. Rhizosphere soil samples were collected by brush. The soil samples were transported to the laboratory for chemical analysis and bacterial community composition and diversity was obtanied through DNA extraction, 16S rRNA gene amplification and high throughput sequencing.Results: The results showed that, compared to seedling plantations, soil quality decreased significantly in coppice stands, but without affecting soil exchangeable Mg2+ and K+. Total carbon (C) and nitrogen (N) were lower in the rhizosphere than in bulk soil, whereas nutrient availability showed an opposite trend. The conversion from seedling to coppice plantations was also related to significant differences in soil bacterial community structure and to the reduction of soil bacterial α-diversity. Principal component analysis (PCA) showed that, bacterial community composition was similar in both bulk and rhizosphere soils in second generation coppice plantations. Specially, the conversion from seedling to coppice increased the relative abundance of Proteobacteria and Rhizobium, but reduced that of Actinobacteria, which may result in a decline of soil nutrient availability. Mantel tests revealed that C, N, Soil organic matter (SOM), nitrate nitrogen (NO3--N) and available phosphorus positively correlated with bacterial community composition, while a variation partition analysis (VPA) showed that NO3--N explained a relatively greater proportion of bacterial distribution (15.12%), compared with C and SOM. Surprinsingly, N showed no relationship with bacterial community composition, which may be related to nitrogen transportation. Conclusions: The conversion from seedling to coppice stands reduced soil quality and led to spatial-temporal homogenization of the soil bacterial community structure in both the rhizosphere and bulk soils. Such imbalance in microbial structure can accelerate the decline of R. pseudoacacia. This may affect the role of R. pseudoacacia coppice stands in soil and forest restoration of barren lands in mountain areas.
Pregnancy Readiness in Married Female Adolescents from Different Ethnic Groups in Probolinggo District, Indonesia

Background: Physical readiness for pregnancy potentially affects the new born’s health status. The study aims to analyze pregnancy readiness in married female adolescents.Methods: Data were collected in Probolinggo District, Indonesia from 2012-2014. This study involved 760 married female adolescents (< 20 years old) as the analysis units. There are 2 major ethnic groups in Probolinggo District (Javanese and Madurese), as well as a small number of other ethnic groups. The study’s dependent variable was pregnancy readiness, while its independent variable was ethnicity. In addition, other independent variables were also analyzed, for example, marital status, age, education, and employment. The final analysis was done using a binary logistic regression. Pregnancy readiness variable was seen from other sub-variables, such as anemia status, body weight, stature, nutritional status, and mid-upper-arm circumference. Results: Married female adolescents mostly suffered from anemia. The respondents mostly had normal anthropometry. Regarding pregancy readiness variable, married female adolescents from all ethnicities married female adolescentswere not ready to get pregnant. Madurese married female adolescents were 0.563 times readier to get pregnant compared to Javanese married female adolescents (OR 0.563; 95% CI 0.369-0.857). There was no significant difference between Madurese ethnic adolescents and the Java-Madurese ones and others. Moreover, there was no significant difference between educational levels and the respondents’ readiness for pregnancy. Conclusions: There were partial differences in readiness for pregancy in married female adolescents from different ethnic groupsmarried female adolescents. Madurese married female adolescents had a lower possibility of prenancy readiness than Javanese ones.
Curcumin Reverses the Scopolomine-induced Cognitive Impairment by iNOS and Acetylcholinesterase Inhibition in Rodent Model of Schizophrenia

Schizophrenia is a severe neuro-developmental psychiatric disorder. Curcumin is a polyphenolic compound extracted from turmeric. It is known for its antioxidant, anti-inflammatory, neuroprotective, and precognitive properties. The purpose of the current study was to evaluate the role of curcumin in scopolamine induced cognitive impairment in animal model of schizophrenia. The elevated plus-maze test was utilised to study the curcumin effect on learning and memory. Curcumin (100 mg/kg, i.p.) was administered daily for 28 days in animals. Behavioural tests such as transfer latency (TL) and spontaneous alteration behaviour was assessed after the last dose of curcumin on the 28th day, followed by biochemical estimations. Present study reported that curcumin showed anti-amnesic effect in animal models of cognitive impairment of schizophrenia. Curcumin reduced the TL compared to toxic control group (scopolamine per se) (P <0.001) in elevated plus maze. In spontaneous alteration behaviour test, curcumin significantly increased percentage alteration and possible alteration as compared to toxic control group (P <0.001). A significant change in acetyl cholinesterase activity, nitrate and oxidative parameters was observed, thus, confirming its anti acetyl cholinesterase, NOS (nitric oxide synthase) inhibition and antioxidant properties (P <0.05). The present study put forward the claim of curcumin as a new and safer therapeutic alternative for the treatment of cognitive impairment in Schizophrenia. The underlying mechanism of this potential effect may be related to anticholinesterase and nitric oxide synthase inhibition activity of curcumin. Further research is warranted for confirming the suggested pathways accountable for memory alleviating effects of curcumin in Schizophrenia.
DETECTION AND CHARACTERIZATION OF IMMUNE COMPLEXES BY THE PLATELET AGGREGATION TEST

SUMMARY Modelexperiments withpreformed antigen-antibody complexes haveshownthat theplatelet aggregation test canbeusedtocharacterize immunecomplexes. The platelet aggregating activity offourserabehaved like antigen-antibody complexes withrespect totheeffect ofaddedantigen, addedantibody andsedimentation in density gradient centrifugation. Twooftheserawerefrompatients withfever of unknown origin. Thefindings suggest that thetwoseracontained circulating immunecomplexes which hadbothmeasles hyperimmunization induced andnormal specificities. Theserumofapatient withfatal subacute hepatitis seemed tocontain immunecomplexes withAustralia antigen. Itwasalso positive inmanyoftheautoimmunity tests. Oneofthesera wasfromapatient withhaemorrhagic varicella inthe acute phase ofthedisease. Thepatient hadreceived large amounts ofy-globulin. The results suggested thetransient presence ofcirculating immunecomplexes withvaricella specificity.
Assessment of matrix effect in quantitative milk analysis for pyrethroids based on GC-MS/MS

In chromatographic analysis of complex samples, the chromatographic responses attributed to pesticides may undergo changes caused by matrix components. These variations can be due by the phenomenon named matrix effect. Cyfluthrin (CYF), cypermethrin (CYP), deltamethrin (DEL), and permethrin (PER) were extracted from raw milk and analysis carried out using a Gas Chromatography coupled to mass spectrometry (GC-MS/MS) technique. The aim of the present work was to investigate the variations of chromatographic response when milk samples are diluted with different solvents (acetone, acetonitrile or hexane) in final dilution immediately after the extraction. Results showed differences between solvents (p<0.05) and acetonitrile showed more appropriate results for all pyrethroids.
Feedforward and Feedback Tracking Control of Nonlinear Diffusion-Convection-Reaction Systems Using Summability Methods

Motion planning and design of feedforward and feedback tracking control are studied for a tubular reactor modeled by nonlinear parabolic diffusion−convection−reaction equations. The approach is based on formal power series parametrizations of the system states and inputs, whereby the domain of convergence of the formal series solutions can be greatly extended using appropriate summation methods. As a result, feedforward controls can be determined for a wide range of trajectories and system parameters. Furthermore, on the basis of a reinterpretation of the formal power series parametrization, state feedback with asymptotic tracking control and an observer can be designed for the tubular reactor. On the other hand, following the 2-degrees-of-freedom approach, the feedforward control can be supplemented by a standard output feedback to obtain robust tracking control for tubular reactors with parameter sets reflecting the unpacked tubular reactor up to the real fixed-bed reactor.
Study of Iron Ion Transit through Three-Fold Channel of Ferritin Cage

Ferritin is an iron-storage globular protein with an ability to uptake, mineralize and release iron ions in a controllable manner. The globular hollow shell allows storage of mineralized iron, with several channels responsible for the transit of ions into the shell and out of it. Understanding of the detailed molecular functioning of ferritin is required for rational design of biomimetic conjugate nano-biosystems containing ferritin-like constituents. In this work, ferritin was investigated both numerically by all-atom molecular dynamics (MD) simulations, and experimentally by Raman spectroscopy. Molecular dynamic simulations of a model system comprising iron ions (Fe) and a ferritin trimer expressing a three-fold channel responsible for the ion transport, have revealed a quick entering of ions in the channel. The transit of iron ions through the channel was thoroughly investigated. The transit was found to be driven by both electrostatic charge of ferritin, and interaction between the ions. Exit (expulsion) of an iron ion from the channel was observed at a condition that at least one more ion is present in the channel. Raman characterization of an iron-loaded ferritin solution revealed pronounced bands attributable to iron, as expected. However, Raman spectra of apoferritin, which does not contain an iron mineral, also exhibited similar bands. Based on the results of MD simulations, it was hypothesized that apo-ferritin retains iron ions in its three-fold channels, and these ions may produce the observed Raman bands. The study of molecular mechanisms involved in the iron ion transit elucidates the pathways of iron uptake and release in ferritin.