Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in the Sensitivity to Language-Specific Orthographic Patterns With Age

How do bilingual readers of languages that have similar scripts identify a language switch? Recent behavioral and electroencephalographic results suggest that they rely on orthotactic cues to recognize the language of the words they read in ambiguous contexts. Previous research has shown that marked words with language-specific letter sequences (i.e., letter sequences that are illegal in one of the two languages) are recognized more easily and faster than unmarked words. The aim of this study was to investigate sensitivity to markedness throughout childhood and early adulthood by using a speeded language decision task with words and pseudowords. A large group of Spanish-Basque bilinguals of different ages (children, preteenagers, teenagers and adults) was tested. Results showed a markedness effect in the second language across all age groups that changed with age. However, sensitivity to markedness in the native language was negligible. We conclude that sensitivity to orthotactics does not follow parallel developmental trend in the first and second language.
Riboflow: Using Deep Learning to Classify Riboswitches With ∼99% Accuracy

Riboswitches are cis-regulatory genetic elements that use an aptamer to control gene expression. Specificity to cognate ligand and diversity of such ligands have expanded the functional repetoire of riboswitches to mediate mounting apt responses to sudden metabolic demands and signal changes in environmental conditions. Given their critical role in microbial life, and novel uses in synthetic biology, riboswitch characterisation remains a challenging computational problem. Here we have addressed the issue with advanced deep learning frameworks, namely convolutional neural networks (CNN), and bidirectional recurrent neural networks (RNN) with Long Short-Term Memory (LSTM). Using a comprehensive dataset of 32 ligand classes and a stratified train-validate-test approach, we demonstrated the superior performance of both the deep models (CNN and RNN) relative to other conventional machine learning classifiers on all key performance metrics, including the ROC curve analysis. In particular, the bidirectional LSTM RNN emerged as the best-performing learning method for identifying the ligand-specificity of riboswitches with an accuracy > 0.99 and macro-averaged F-score of 0.96. A dynamic update functionality is inbuilt to account for the discovery of new riboswitches and extend the predictive modelling to any number of new additional classes. Our work would be valuable in the design and assembly of genetic circuits and the development of the next generation of antibiotics. The software is freely available as a Python package and standalone resource for wide use in genome annotation and biotechnology workflows. Availability PyPi package: riboflow @ https://pypi.org/project/riboflow Repository with Standalone suite of tools: https://github.com/RiboswitchClassifier Language: Python 3.6 with numpy, keras, and tensorflow libraries. Licence: MIT
Automatic Reappraisal-Based Implementation Intention Produces Early and Sustainable Emotion Regulation Effects: Event-Related Potential Evidence

Implementation intention has proven effective in regulating intense emotions but is found to be difficult when instructed regulation is used. Here, we aim to test whether automatic reappraisal-based implementation intention (RII) downregulates intense negative emotion more efficiently than controlled reappraisal (CR) using a two-phase event-related potential decreased subjective experiences of negative emotion relative to passive watching, irrespective of emotional intensity. Moreover, RII reduced the central–parietal late positive potential (LPP) amplitudes for both intensities in the 300–1,700-ms epoch after picture onset, whereas CR reduced LPP amplitudes just in the 500–700-ms interval. Moreover, the application of RII but not CR produced a reliable long-term LPP attenuation compared to passive watching in the unexpected re-exposure phase. These findings demonstrate that reappraisal-based implementation intention yields an earlier and more sustainable emotion regulatory effects than controlled reappraisal.
Processing Surface EMG Signals for Exoskeleton Motion Control

The surface electromyography (sEMG) signal has been used for volitional control of robotic assistive devices. There are still challenges in improving system performance accuracy and signal processing to remove systematic noise. This study presents procedures and a pilot validation of the EMG-driven speed-control of exoskeleton and integrated treadmill with a goal to provide better interaction between a user and the system. The gait cycle duration (GCD) was extracted from sEMG signals using the autocorrelation algorithm and Bayesian fusion algorithm. GCDs of various walking speeds were then programmed to control the motion speed of exoskeleton robotic system. The performance and efficiency of this sEMG-controlled robotic assistive ambulation system was tested and validated among 6 healthy volunteers. The results demonstrated that the autocorrelation algorithm extracted the GCD from individual muscle contraction. The GCDs of individual muscles had variability between different walking steps under a designated walking speed. Bayesian fusion algorithms processed the GCDs of multiple muscles yielding a final GCD with the least variance. The fused GCD effectively controlled the motion speeds of exoskeleton and treadmill. The higher amplitude of EMG signals with shorter GCD was found during a faster walking speed. The algorithms using fused GCDs and gait stride length yielded trajectory joint motion tracks in a shape of sine curve waveform. The joint angles of the exoskeleton measured by a decoder mounted on the hip turned out to be in sine waveforms. The hip joint motion track of the exoskeleton matched the angles projected by trajectory curve generated by computer algorithms based on the fused GCDs with high agreement. The EMG-driven speed-control provided the human-machine inter-limb coordination mechanisms for an intuitive speed control of the exoskeleton-treadmill system at the user’s intents. Potentially the whole system can be used for gait rehabilitation of incomplete spinal cord hemispheric stroke patients as goal-directed and task-oriented training tool.
ZFP36 Binds With PRC1 to Inhibit Tumor Growth and Increase 5-Fu Chemosensitivity of Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is the fifth common cause of tumor-related death worldwide. ZFP36, a RNA-binding protein, decreases in many cancers and its role in HCC remains unclear. This study aimed to investigate the underlying mechanisms by which ZFP36 inhibited HCC progression and increased fluorouracil (5-Fu) sensitivity. We found that ZFP36 was downregulated and PRC1 was upregulated in HCC tissues compared with adjacent non-tumor tissues. In vitro investigation presented that ZFP36 acted as a tumor suppressor, while overexpression of PRC1 increased cell proliferation, colony formation and invasion. Further investigations demonstrated that overexpression of ZFP36 inhibited tumor growth and promoted 5-Fu sensitivity in xenograft tumor mice model, which could be reversed when PRC1 overexpressed simultaneously. Luciferase reporter assays and Ribonucleoprotein immunoprecipitation analysis indicated that ZFP36 could bind to adenylate uridylate-rich elements located in PRC1 mRNA 3′UTR to downregulate PRC1 expression. Taken together, our findings identified that ZFP36 regulated PRC1 to exert anti-tumor effect, which suggested a potential therapeutic strategy for treating HCC by exploiting ZFP36/PRC1 axis.
The Effects of Acute Moderate and High Intensity Exercise on Memory

Acute cardiovascular exercise can enhance correct remembering but its impact upon false remembering is less clear. In two experiments, we investigated the effect of acute bouts of exercise on correct and false remembering using the Deese–Roediger–McDermott (DRM) memory test. In Experiment 1, healthy adults completed quiet rest or moderate intensity cycling prior to the memory test. In Experiment 2, a similar sample completed moderate intensity running, high intensity sprints, or a period of quiet rest prior to the memory test. In Experiment 1, acute moderate intensity exercise increased short-term correct, but not false, recall. Experiment 2 replicated these findings but also found an acute bout of high intensity exercise had no impact upon either type of short-term recall. Acute moderate intensity exercise, but not acute high intensity exercise, can improve short-term correct recall without an accompanying increase in false recall potentially through processing of contextually specific information during encoding.
Targeting of the C-Type Lectin Receptor Langerin Using Bifunctional Mannosylated Antigens

Langerhans cells (LCs) are antigen-presenting cells that reside in the skin. They uniquely express high levels of the C-type lectin receptor Langerin (CD207), which is an attractive target for antigen delivery in immunotherapeutic vaccination strategies against cancer. We here assess a library of 20 synthetic, well-defined mannoside clusters, built up from one, two, and three of six monomannosides, dimannosides, or trimannosides, appended to an oligopeptide backbone, for binding with Langerin using surface plasmon resonance and flow cytometric quantification. It is found that Langerin binding affinity increases with increasing number of mannosides. Hexavalent presentation of the mannosides resulted in binding affinities ranging from 3 to 12 μM. Trivalent presentation of the dimannosides and trimannosides led to Langerin affinity in the same range. The model melanoma gp100 antigenic peptide was subsequently equipped with a hexavalent cluster of the dimannosides and trimannosides as targeting moieties. Surprisingly, although the bifunctional conjugates were taken up in LCs in a Langerin-dependent manner, limited antigen presentation to cytotoxic T cells was observed. These results indicate that targeting glycan moieties on immunotherapeutic vaccines should not only be validated for target binding, but also on the continued effects on biology, such as antigen presentation to both CD8+ and CD4+ T cells.
Characterization of Na+ exclusion mechanisms of salt‐tolerant reed plants in comparison with salt‐sensitive rice plants

Salt-tolerant reed plants (Phragmites communis Trinius) and salt-sensitive rice plants (Oryza sativa L. cv. Kinmaze) were grown in salinized nutrient solutions up to 50 mM NaCl, and growth, Na+ contents and kinetics of 22Na+ uptake and translocation were compared between the species to characterize the salt tolerance mechanisms operating in reed plants. When both plants were grown under the same salinity, Na+ contents of the shoots were lower in reed plants, although those of the roots were quite similar. The shoot base region of both species accumulated Na+ more than the leaf blades did. Sodium-22 uptake and pulse-chase experiments suggested that the lower Na+ transport rate from root to shoot could limit excessive Na+ accumulation in the reed shoot. There was a possibility that the apparently lower 22Na+ transport rate to the shoot of reed plants was due to net downward Na+ transport from shoot base to root.
A Simple Assay to Assess Salmonella enterica Persistence in Lettuce Leaves After Low Inoculation Dose

Salmonella enterica is an enterobacterium associated with numerous foodborne illnesses worldwide. Leafy greens have been a common vehicle for disease outbreaks caused by S. enterica. This human pathogen can be introduced into crop fields and potentially contaminate fresh produce. Several studies have shown that S. enterica can survive for long periods in the plant tissues. Often, S. enterica population does not reach high titers in leaves; however, it is still relevant for food safety due to the low infective dose of the pathogen. Thus, laboratory procedures to study the survival of S. enterica in fresh vegetables should be adjusted accordingly. Here, we describe a protocol to assess the population dynamics of S. enterica serovar Typhimurium 14028s in the leaf apoplast of three cultivars of lettuce (Lactuca sativa L.). By comparing a range of inoculum concentrations, we showed that vacuum infiltration of a bacterium inoculum level in the range of 3.4 Log CFU ml–1 (with a recovery of approximately 170 cells per gram of fresh leaves 2 h post inoculation) allows for a robust assessment of bacterial persistence in three lettuce cultivars using serial dilution plating and qPCR methods. We anticipate that this method can be applied to other leaf–human pathogen combinations in an attempt to standardize the procedure for future efforts to screen for plant phenotypic variability, which is useful for breeding programs.
Sexually Transmitted Infections Among Kenyan Adolescent Girls and Young Women With Limited Sexual Experience

Objectives: Globally, the highest rates of sexually transmitted infections (STIs) are among the 15-24 age group. Studying adolescent girls and young women (AGYW) pre-sexual debut could identify risk factors for STI acquisition. Methods: We recruited a prospective cohort of low-risk AGYW aged 16-20 in Kenya. Participants were HIV and HSV-2 seronegative and reported no history of sexual intercourse or reported sex with one partner. Participants underwent genital exams, nucleic acid testing of vaginal swabs for Neisseria gonorrhea (NG), Chlamydia trachomatis (CT), Trichomonas vaginalis (TV), and vaginal gram stains for vaginal dysbiosis by Nugent score. STI correlates were described using χ2 test and t-test. Results: We enrolled 400 AGYW, of which 322 (80.5%) reported never having had sex, while 78 (19.5%) reported prior sex with 1 partner. Among the 78 participants reporting prior sex, 20 (25.6%) reported contraception use in the last 3 months, with 60% using only emergency contraceptive pills. Despite self-reported history, of 373 subjects who underwent STI testing, 49 subjects (13.1%) tested positive for STIs, with 41 CT, 5 GC, and 3 TV cases. Of these 49 subjects, 33 (67.3%) reported no prior sexual intercourse. Bacterial vaginosis was rare and 90% of subjects had a normal Nugent score (0–3). Conclusions: Upon baseline evaluation of a cohort of low risk AGYW, we found high numbers of STIs, especially CT, which is not routinely screened for in Kenyan settings. Interventions to address STIs and unintended pregnancy should target girls pre-sexual debut, including those who do not self-identify as at risk.
Current State of Target Treatment in BRAF Mutated Melanoma

Incidence of melanoma has been constantly growing during the last decades. Although most of the new diagnoses are represented by thin melanomas, the number of melanoma-related deaths in 2018 was 60,712 worldwide (Global Cancer Observatory, 2019). Until 2011, no systemic therapy showed to improve survival in patients with advanced or metastatic melanoma. At that time, standard of care was chemotherapy, with very limited results. The identification of BRAF V600 mutation, and the subsequent introduction of BRAF targeting drugs, radically changed the clinical practice and dramatically improved outcomes. In this review, we will retrace the development of molecular-target drugs and the current therapeutic scenario for patients with BRAF mutated melanoma, from the introduction of BRAF inhibitors as single agents to modern clinical practice. We will also discuss the resistance mechanisms identified so far, and the future therapeutic perspectives in BRAF mutated melanoma.
How Numerical Unbalance Constraints Physical and Tactical Individual Demands of Ball Possession Small-Sided Soccer Games

This study aimed to explore the effects of playing different unbalanced ball possession small-sided games on external workload (distance covered while walking, running, and sprinting, and max speed), tactical individual actions (number of passes with dominant and non-dominant foot), and internal load (rating of perceived exertion, RPE) in under-23 soccer players. Participants played 4v2, 4v3, 4v4, 4v5, and 4v6 small-sided games (SSGs) on a 30 × 25 m playing area. Data were analyzed under an opponent-based perspective, by fixing one team (4vX), and by cooperation-based perspective according to teammates (4v2+X). Global Position System (GPS) monitors were used to collect and compute external workloads and individual tactical actions, and Borg Scale CR10 was used to evaluate RPE. High-Superiority (4v2), Superiority (4v3), and Very Low-Cooperation (4v2+0) formats allow players in balanced teams to cover more distance while walking; on the other side, Inferiority (4v5), High-Inferiority (4v6), and Very High-Cooperation (4v2+4) allow players to sprint more and practice more tactical individual actions as a resultant emergent behavior; all players in SSG conditions with a lower number of conditions, perceived the exercise as more intense, especially in situations with less than two players. Overall, playing in high inferiority situations (4v2 and 4v6) may be used to increase physical demand for the outnumbered team, while coaches may use low superiority situations to adjust the task complexity when developing the players’ tactical individual actions.
Static and Dynamic Changes of Amplitude of Low-Frequency Fluctuations in Cervical Discogenic Pain

Cervical discogenic pain (CDP) is a clinically common pain syndrome caused by cervical disk degeneration. A large number of studies have reported that CDP results in brain functional impairments. However, the detailed dynamic brain functional abnormalities in CDP are still unclear. In this study, using resting-state functional magnetic resonance imaging, we explored the neural basis of CDP with 40 CDP patients and 40 age-, gender-matched healthy controls to delineate the changes of the voxel-level static and dynamic amplitude of low frequency fluctuations (ALFF). We found increased static ALFF in left insula (INS) and posterior precuneus (PCu), and decreased static ALFF in left precentral/postcentral gyrus (PreCG/PoCG), thalamus (THA), and subgenual anterior cingulate cortex in CPD patients compared to healthy controls. We also found decreased dynamic ALFF in left PreCG/PoCG, right posterior middle temporal gyrus, and bilateral THA. Moreover, we found that static ALFF in left PreCG/PoCG and dynamic ALFF in THA were significantly negatively correlated with visual analog scale and disease duration, respectively. Our findings provide the neurophysiological basis for CDP and facilitate understanding the neuropathology of CDP.
Abnormalities in Brain and Muscle Microstructure and Neurochemistry of the DMD Rat Measured by in vivo Diffusion Tensor Imaging and High Resolution Localized 1H MRS

Duchenne muscular dystrophy (DMD) is an X-linked disorder caused by the lack of dystrophin with progressive degeneration of skeletal muscles. Most studies regarding DMD understandably focus on muscle, but dystrophin is also expressed in the central nervous system, potentially resulting in cognitive and behavioral changes. Animal models are being used for developing more comprehensive neuromonitoring protocols and clinical image acquisition procedures. The recently developed DMD rat is an animal model that parallels the progressive muscle wasting seen in DMD. Here, we studied the brain and temporalis muscle structure and neurochemistry of wild type (WT) and dystrophic (DMD) rats using magnetic resonance imaging and spectroscopy. Both structural and neurochemistry alterations were observed in the DMD rat brain and the temporalis muscle. There was a decrease in absolute brain volume (WT = 1579 mm3; DMD = 1501 mm3; p = 0.039, Cohen’s d = 1.867), but not normalized (WT = 4.27; DMD = 4.02; p = 0.306) brain volume. Diffusion tensor imaging (DTI) revealed structural alterations in the DMD temporalis muscle, with increased mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD). In the DMD rat thalamus, DTI revealed an increase in fractional anisotropy (FA) and a decrease in RD. Smaller normalized brain volume correlated to severity of muscle dystrophy (r = −0.975). Neurochemical changes in the DMD rat brain included increased GABA and NAA in the prefrontal cortex, and GABA in the hippocampus. Such findings could indicate disturbed motor and sensory signaling, resulting in a dysfunctional GABAergic neurotransmission, and an unstable osmoregulation in the dystrophin-null brain.
Characterization of bioactive crude from culture broth of Brevibacillus laterosporus SA14 against clinical isolates of methicillin-resistant Staphylococcus aureus and in vitro cytotoxicity to human colon cancer HT-29 cells

Brevibacillus laterosporus SA14 excreted the antimicrobial peptides into culture broth on the 1 st day of cultivation. The antimicrobial peptides in 1-day culture broth were precipitated by using ammonium sulfate of 50% saturation, named as bioactive crude. Its characteristic measurements indicated that the bioactive crude had a relatively gram-positive bacteria with inhibitory spectrum especially Staphylococcus aureus TISTR 517 and tested MRSAs by agar well diffusion. The MICs of bioactive crude (ranged from 0.003 to 0.049 mg/ml) against all tested MRSAs was documented. The anti-MRSA activity of bioactive crude was not affected by a wide pH, chemical agents and temperature range. Bioactive crude at the concentration used against MRSA did not cause toxicity to human colon cancer HT-29 cells. Its activity of bioactive compound against MRSAs would provide further evaluation of known chemical structure and may be helpful for its clinical implication.
Cerebrovascular Reactivity After Sport Concussion: From Acute Injury to 1 Year After Medical Clearance

Neuroimaging has identified significant disturbances in cerebrovascular reactivity (CVR) in the early symptomatic phase of sport-related concussion. However, less is known about how whole-brain alterations in CVR evolve after concussion and whether they remain present beyond medical clearance to return to play (RTP). In the present study, CVR was evaluated using blood-oxygenation-level-dependent functional magnetic resonance imaging (BOLD fMRI) during a respiratory challenge. Imaging data were collected for 110 university-level athletes, including 39 concussed athletes and 71 athletic controls. The concussed athletes were imaged at the acute phase of injury (1–7 days post-injury), the subacute phase (8-14 days post-injury), medical clearance to RTP, 1 month post-RTP, and 1 year post-RTP. Enhanced negative BOLD response to controlled breathing was seen at acute injury, with attenuation of the effect mainly occurring by 1 year post-RTP. Secondary analyses showed that greater symptom severity and prolonged recovery were associated with enhanced BOLD response in the acute phase of injury, but a more attenuated BOLD response in the subacute phase. This study provides novel information characterizing the CVR response after concussion and shows CVR to be a sensitive technique for evaluating long-term brain recovery.