Miracle-dz/UniFAR-FTUs huggingface dataset:
The Role of the Xylem in Oxytetracycline Translocation within Citrus Trees

Antibiotics have been successfully used to control plant diseases for more than fifty years. Recently, oxytetracycline and streptomycin have been approved for the treatment of Huanglongbing, which is threatening the citrus industry in many regions. Because the efficiency of antibiotics in planta is highly affected by their movement and distribution, understanding the mechanism of antibiotics’ uptake and distribution could lead to a better control of plant pathogens. Herein, we investigated the movement of oxytetracycline within citrus plants. Oxytetracycline was applied by root drenching to both girdled and non-girdled citrus seedlings. In addition, oxytetracycline was applied by trunk injection to girdled and non-girdled citrus trees. After the exposure time (24 h), citrus seedlings were dissected and the levels of oxytetracycline in the different tissues were measured using an oxytetracycline ELISA kit. Upon root application (laboratory experiment), oxytetracycline was detected in the inner part of the stem (xylem-associated tissue), cortex (phloem-associated tissue), and leaves above and below the girdled area. Likewise, oxytetracycline was also detected in leaves of trunk-injected field trees (girdled and non-girdled) three days post treatment. Interestingly, cortex girdling did not affect the distribution and translocation of oxytetracycline, indicating that the xylem is the main path for oxytetracycline translocation. Taken together, our results indicate that oxytetracycline translocation mainly occurs via xylem vessels, and that movement into the phloem occurs subsequent to xylem translocation. Our findings also clearly demonstrated that upon trunk injection, only trace levels of oxytetracycline reached the roots, minimizing its therapeutic value there. Thus, our recommendation is to time tree injections to coincide with the flushing periods when the bacteria are moving into new shoots to maximize the efficiency of oxytetracycline.
Identifying emerging mental illness utilizing search engine activity: A feasibility study

Mental illness often emerges during the formative years of adolescence and young adult development and interferes with the establishment of healthy educational, vocational, and social foundations. Despite the severity of symptoms and decline in functioning, the time between illness onset and receiving appropriate care can be lengthy. A method by which to objectively identify early signs of emerging psychiatric symptoms could improve early intervention strategies. We analyzed a total of 405,523 search queries from 105 individuals with schizophrenia spectrum disorders (SSD, N = 36), non-psychotic mood disorders (MD, N = 38) and healthy volunteers (HV, N = 31) utilizing one year’s worth of data prior to the first psychiatric hospitalization. Across 52 weeks, we found significant differences in the timing (p<0.05) and frequency (p<0.001) of searches between individuals with SSD and MD compared to HV up to a year in advance of the first psychiatric hospitalization. We additionally identified significant linguistic differences in search content among the three groups including use of words related to sadness and perception, use of first and second person pronouns, and use of punctuation (all p<0.05). In the weeks before hospitalization, both participants with SSD and MD displayed significant shifts in search timing (p<0.05), and participants with SSD displayed significant shifts in search content (p<0.05). Our findings demonstrate promise for utilizing personal patterns of online search activity to inform clinical care.
Response of Palladium and Carbon Nanotube Composite Films to Hydrogen Gas and Behavior of Conductive Carriers

To develop a high-performance hydrogen gas sensor, we fabricated a composite film made of carbon nanotubes (CNTs) and palladium nanoparticles. Carbon nanotubes were spin-coated onto a glass substrate, and subsequently, palladium nanoparticles were sputtered onto this film. The response to hydrogen gas was measured during two seasons (summer and winter) using a vacuum chamber by introducing a hydrogen/argon gas mixture. There was a clear difference in the sensor response despite the temperature difference between summer and winter. In addition, since a clean chamber was used, fewer water molecules acted as a dopant, and the behavior of the CNT changed from p-type to n-type because of the dissociative adsorption of hydrogen. This phenomenon was confirmed as the Seebeck effect. Finally, the work functions of Pd, PdHx, and CNT were calculated by first-principle calculations. As predicted by previous studies, a decrease in work function due to hydrogen adsorption was confirmed; however, the electron transfer to CNT was not appropriate from the perspective of charge neutrality and was found to be localized at the Pd/CNT interface. It seems that the Seebeck effect causes the concentration of conductive carriers to change.
Communication, Trust and Dental Anxiety: A Person-Centred Approach for Dental Attendance Behaviours

Effective communication forges the dentist-patient treatment alliance and is thus essential for providing person-centred care. Social rank theory suggests that shame, trust, communication and anxiety are linked together, they are moderated by socio-economic position. The study is aimed to propose and test an explanatory model to predict dental attendance behaviours using person-centred and socio-economic position factors. A secondary data analysis was conducted on a cross-sectional representative survey of a two-stage cluster sample of adults including England, Wales and Northern Ireland. Data were drawn from structured interview. Path analysis of proposed model was calculated following measurement development and confirmation of reliable constructs. The findings show model fit was good. Dental anxiety was predicted negatively by patient’s trust and positively by reported dentist communication. Patient’s shame was positively associated with dental anxiety, whereas self-reported dental attendance was negatively associated with dental anxiety. Both patient’s trust and dentist’s communication effects were moderated by social class. Manual classes were most sensitive to the reported dentist’s communications. Some evidence for the proposed model was found. The relationships reflected in the model were illuminated further when social class was introduced as moderator and indicated dentists should attend to communication processes carefully across different categories of patients.
Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.
Accuracy evaluation of 3D printed interim prosthesis fabrication using a CBCT scanning based digital model

Objectives This study aimed to evaluate the marginal and internal gaps in 3D-printed interim crowns made from digital models of cone-beam computed tomography (CBCT) conversion data. Materials and methods Sixteen polyvinylsiloxane impressions were taken from patients for single crown restorations and were scanned using CBCT. The scanning data were converted to positive Standard Triangulation Language (STL) files using custom-developed software. The fabricated stone models were scanned with an intraoral optical scanner (IOS) to compare the surface accuracy with the STL data obtained by CBCT. The converted STL files were utilized to fabricate interim crowns with a photopolymer using a digital light-processing 3D printer. The replica method was used to analyze the accuracy. The marginal and internal gaps in the replica specimen of each interim crown were measured with a digital microscope. The Friedman test and Mann-Whitney U test (Wilcoxon-signed rank test) were conducted to compare the measurements of the marginal and internal gaps with a 95% level of confidence. Results The root-mean-square values of the CBCT and IOS ranged from 41.00 to 126.60 μm, and the mean was 60.12 μm. The mean values of the marginal, internal, and total gaps were 132.96 (±139.23) μm, 137.86 (±103.09) μm, and 135.68 (±120.30) μm, respectively. There were no statistically significant differences in the marginal or internal gaps between the mesiodistal and buccolingual surfaces, but the marginal area (132.96 μm) and occlusal area (255.88 μm) had significant mean differences. Conclusion The marginal gap of the fabricated interim crowns based on CBCT STL data was within the acceptable range of clinical success. Through ongoing developments of high-resolution CBCT and the digital model conversion technique, CBCT might be an alternative method to acquire digital models for interim crown fabrication.
Effects of Combined Strength and Resisted Sprint Training on Physical Performance in U-19 Elite Soccer Players

Abstract Ben Brahim, M, Bougatfa, R, Makni, E, Gonzalez, PP, Yasin, H, Tarwneh, R, Moalla, W, and Elloumi, M. Effects of combined strength and resisted sprint training on physical performance in U-19 elite soccer players. J Strength Cond Res 35(12): 3432–3439, 2021—This study assessed the effects of combined muscular strength and resisted sprint training using both sled and weight vest compared with regular soccer training on physical fitness of lower limbs in U-19 elite soccer players. Thirty-four male soccer players (age: 18.8 ± 0.8 years, height: 1.81 ± 0.05 m, body mass: 76.4 ± 4.9 kg, and body fat mass: 11.3 ± 4.2%) were randomly assigned into a resisted sprint training group (RSTG, n = 20), using both weight vest and sled, and a control group (CONTG, n = 14). Sprinting ability (5 m and 20 m), squat jump (SJ) and counter-movement jump (CMJ) tests, 1 repetition maximum of half-back squat (1RM half-back squat), and soccer ball-shooting speed were assessed before and after a 6-week training program. Within-group interactions showed significant combined muscular strength and resisted sprint training effects were observed for all the tests' measurements (effect sizes = 0.97 and 3.69 for 20-m sprint and SJ, respectively). However, significant increases of performances were observed for 5-m and 20-m sprinting time (ηp2 = 0.25, p < 0.01 and ηp2 = 0.22, p < 0.01, respectively), SJ and CMJ (ηp2 = 0.78, p < 0.0001 and ηp2 = 0.34, p < 0.001, respectively), 1 repetition maximum (1-RM) half-back squat (ηp2 = 0.45, p < 0.0001), and soccer ball-shooting speed (ηp2 = 0.41, p < 0.0001) in RSTG with large effect size, whereas the CONTG showed significant performances increase only for CMJ (p < 0.05), 1RM half-back squat (p < 0.01), and soccer ball-shooting speed (p < 0.05). We conclude that combined strength and both horizontal (weighted sled) and vertical (weighted vest) resisted sprint training are more effective than regular soccer training for enhancing sprinting and jumping abilities as well as ball-shooting speed in soccer.
Effects of the probiotic Bacillus amyloliquefaciens on growth performance, hematology and intestinal morphometry in cage-reared Nile tilapia

El objetivo de este estudio fue evaluar el efecto del probiotico Bacillus amyloliquefaciens en el desempeno del crecimiento, hemograma y morfometria intestinal en tilapia del Nilo (Oreochromis niloticus) criadas en balsas jaulas. 936 tilapias del Nilo se distribuyeron en 12 balsas jaulas (1,5 m3). Los peces fueron alimentados durante 90 dias con dietas que contenian 0 (control); 1x 106 UFC g-1; 5x106 UFC g-1 y 1x107 UFC g-1 del probiotico. Los resultados no mostraron diferencias significativas en el rendimiento y composicion proximal de los peces. La glucosa en sangre y hemoglobina fue menor en el grupo 1x107 UFC g-1, lo que sugiere un mejor estado homeostatico de los peces. Otros parametros hematimetricos no difirieron entre los grupos. Se observo aumento significativo de la altura de las vellosidades y numero de celulas caliciformes del intestino en los peces suplementados con 5x106 UFC g-1 y 1x 107 UFC g-1 de alimento que sugieren que los peces mejoraron la digestion y absorcion de nutrientes. Sin embargo, se necesitan mas estudios para determinar la eficacia de este probiotico en condiciones de campo en tilapia del Nilo.
Analysis of Spatio-Temporal Transcriptome Profiles of Soybean (Glycine max) Tissues during Early Seed Development

Soybean (Glycine max) is an important crop providing oil and protein for both human and animal consumption. Knowing which biological processes take place in specific tissues in a temporal manner will enable directed breeding or synthetic approaches to improve seed quantity and quality. We analyzed a genome-wide transcriptome dataset from embryo, endosperm, endothelium, epidermis, hilum, outer and inner integument and suspensor at the global, heart and cotyledon stages of soybean seed development. The tissue specificity of gene expression was greater than stage specificity, and only three genes were differentially expressed in all seed tissues. Tissues had both unique and shared enriched functional categories of tissue-specifically expressed genes associated with them. Strong spatio-temporal correlation in gene expression was identified using weighted gene co-expression network analysis, with the most co-expression occurring in one seed tissue. Transcription factors with distinct spatiotemporal gene expression programs in each seed tissue were identified as candidate regulators of expression within those tissues. Gene ontology (GO) enrichment of orthogroup clusters revealed the conserved functions and unique roles of orthogroups with similar and contrasting expression patterns in transcript abundance between soybean and Arabidopsis during embryo proper and endosperm development. Key regulators in each seed tissue and hub genes connecting those networks were characterized by constructing gene regulatory networks. Our findings provide an important resource for describing the structure and function of individual soybean seed compartments during early seed development.
Brain stem auditory-evoked response in the nonanesthetized horse and pony.

: The brain stem auditory-evoked response (BAER) was measured in 10 horses and 7 ponies under conditions suitable for clinical diagnostic testing. Latencies of 5 vertex-positive peaks and interpeak latency and amplitude ratio on the 1st and 4th peaks were determined. Data from horses and ponies were analyzed separately and were compared. The stimulus was a click (n = 3,000) ranging from 10- to 90-dB hearing level (HL). Neither horses nor ponies responded with a BAER at 10 dB nor did they give reliable responses at less than 50 dB. The 2nd of the BAER waves appeared in the record at lower stimulus intensities than did the 1st wave for the horse and pony. Horses and ponies had a decreasing latency for all waves, as a result of increasing stimulus intensity. Latencies were shorter for the ponies than for the horses at all stimulus intensities for the 1st, 2nd, 3rd, and 4th waves, but not the 5th wave. At 60-dB HL, the mean latencies for the 1st through 5th wave, respectively, for the horse were 1.73, 3.08, 3.93, 4.98, and 6.00 ms and for the pony 1.48, 2.73, 3.50, 4.56, and 6.58 ms. Interpeak latencies, 1st to 4th wave, averaged 3.22 ms (horse) and 3.11 ms (pony) for all stimulus intensities from 50- to 90-dB HL and had a tendency to decrease slightly as stimulus intensity increased. Amplitude ratios (4th wave/1st wave) were less than 1 for all stimulus intensities in the horse. In the pony, the ratio was less than 1 at greater than or equal to 70-dB HL and greater than 1 at less than or equal to 60-dB HL.
Effective Melanoma Recognition Using Deep Convolutional Neural Network with Covariance Discriminant Loss

Melanoma recognition is challenging due to data imbalance and high intra-class variations and large inter-class similarity. Aiming at the issues, we propose a melanoma recognition method using deep convolutional neural network with covariance discriminant loss in dermoscopy images. Deep convolutional neural network is trained under the joint supervision of cross entropy loss and covariance discriminant loss, rectifying the model outputs and the extracted features simultaneously. Specifically, we design an embedding loss, namely covariance discriminant loss, which takes the first and second distance into account simultaneously for providing more constraints. By constraining the distance between hard samples and minority class center, the deep features of melanoma and non-melanoma can be separated effectively. To mine the hard samples, we also design the corresponding algorithm. Further, we analyze the relationship between the proposed loss and other losses. On the International Symposium on Biomedical Imaging (ISBI) 2018 Skin Lesion Analysis dataset, the two schemes in the proposed method can yield a sensitivity of 0.942 and 0.917, respectively. The comprehensive results have demonstrated the efficacy of the designed embedding loss and the proposed methodology.
Exploring Aurone Derivatives as Potential Human Pancreatic Lipase Inhibitors through Molecular Docking and Molecular Dynamics Simulations

Inhibition of human pancreatic lipase, a crucial enzyme in dietary fat digestion and absorption, is a potent therapeutic approach for obesity treatment. In this study, human pancreatic lipase inhibitory activity of aurone derivatives was explored by molecular modeling approaches. The target protein was human pancreatic lipase (PDB ID: 1LPB). The 3D structures of 82 published bioactive aurone derivatives were docked successfully into the protein catalytic active site, using AutoDock Vina 1.5. 7.rc1. Of them, 62 compounds interacted with the key residues of catalytic trial Ser152-Asp176-His263. The top hit compound (A14), with a docking score of −10.6 kcal⋅mol−1, was subsequently submitted to molecular dynamics simulations, using GROMACS 2018.01. Molecular dynamics simulation results showed that A14 formed a stable complex with 1LPB protein via hydrogen bonds with important residues in regulating enzyme activity (Ser152 and Phe77). Compound A14 showed high potency for further studies, such as the synthesis, in vitro and in vivo tests for pancreatic lipase inhibitory activity.
Phosphoinositide-3-Kinase γ Is Not a Predominant Regulator of ATP-Dependent Directed Microglial Process Motility or Experience-Dependent Ocular Dominance Plasticity

Visual Abstract Microglia are dynamic cells whose extensive interactions with neurons and glia during development allow them to regulate neuronal development and function. The microglial P2Y12 receptor is crucial for microglial responsiveness to extracellular ATP and mediates numerous microglial functions, including ATP-dependent directional motility, microglia-neuron interactions, and experience-dependent synaptic plasticity. However, little is known about the downstream signaling effectors that mediate these diverse actions of P2Y12. Phosphoinositide-3-kinase γ (PI3Kγ), a lipid kinase activated downstream of Gi-protein-coupled receptors such as P2Y12, could translate localized extracellular ATP signals into directed microglial action and serve as a broad effector of P2Y12-dependent signaling. Here, we used pharmacological and genetic methods to manipulate P2Y12 and PI3Kγ signaling to determine whether inhibiting PI3Kγ phenocopied the loss of P2Y12 signaling in mouse microglia. While pan-inhibition of all PI3K activity substantially affected P2Y12-dependent microglial responses, our results suggest that PI3Kγ specifically is only a minor part of the P2Y12 signaling pathway. PI3Kγ was not required to maintain homeostatic microglial morphology or their dynamic surveillance in vivo. Further, PI3Kγ was not strictly required for P2Y12-dependent microglial responses ex vivo or in vivo, although we did observe subtle deficits in the recruitment of microglial process toward sources of ATP. Finally, PI3Kγ was not required for ocular dominance plasticity, a P2Y12-dependent form of experience-dependent synaptic plasticity that occurs in the developing visual cortex. Overall, our results demonstrate that PI3Kγ is not the major mediator of P2Y12 function in microglia, but may have a role in amplifying or fine-tuning the chemotactic response.
Improvements in Flow Cytometry‐Based Cytotoxicity Assay

The flow cytometry‐based assay has been increasingly used to assess the cell‐mediated cytotoxicity since the 1980s due to its advantages over the conventional radioactive 51Cr release assay (CRA), such as higher sensitivity at the single‐cell level and nonradioactivity. The basic principle of this assay is the usage of two dyes, one nontoxic dye for labeling targets or effector cells to distinguish one from another, one viability dye for discrimination of dead from live cells. Due to the problem of spontaneous release or leakage of the nontoxic dye, the concern about the cross‐staining has not yet been clearly elucidated. In this study, carboxyfluorescein diacetate succinimidyl ester (CFSE) was utilized to label target cells and Hoechst 33258 was used as the viability dye. We confirmed that no cross‐staining occurred between the effector and target cells after 4 h of coculture. We also found that the cytotoxicity would be overestimated if effector cells instead of target cells were labeled due to the exclusion of viable targets in effector‐target conjugates. Using EDTA at the end of culture or labeling targets can solve this problem. Furthermore, the gating strategy could be improved by plotting CFSE against forward scatter (FSC) to discriminate some early apoptotic events. Due to the loss of target cells lysed by effector cells, counting beads are normally preferable in this assay. Here, we found an alternative to the use of beads in standardizing the flow cytometry‐based assay. Instead of using beads, sample acquisition in a fixed time was shown to have the same effect in specific lysis evaluation as the beads application but have a greater stability than the latter. With a good quality control, the acquisition time for each sample could be shortened to 15 s, thus making this work to be done efficiently, especially in the case of larger sample sizes. Collectively, the findings in this study can improve the flow cytometric cytotoxicity assay to be carried out in a more accurate, efficient, and cost‐effective way. © 2020 International Society for Advancement of Cytometry