Miracle-dz/UniFAR-FTUs huggingface dataset:
Osteogenesis and expression of the bone marrow niche in endothelial cell‐depleted HipOPs

The identification and purification of murine multipotent mesenchymal stem cells (MSCs) have been difficult due to their low frequency, the presence of contaminating cell types and lack of unambiguous markers. Using a magnetic micro‐beads negative selection technique to remove hematopoietic cells from mouse bone marrow stromal cells (BMSCs), our lab recently isolated a highly purified osteoprogenitor (HipOP) population that was also enriched for other mesenchymal precursors, including MSCs [Itoh and Aubin, 2009 ]. We now report that HipOPs are also highly enriched in vascular endothelial cells (VECs), which we hypothesized were an accessory cell type regulating osteogenesis. However, when VECs were immunodepleted from HipOPs with anti‐CD31 antibodies, the resulting CD31(−) HipOP population had equal osteogenic capacity to the HipOPs in vitro and in vivo. Analysis of gene expression of Ncad, Pth1r, Ang1, Cxcl12, Jag1, Pdgfr‐β, α‐sma, Desmin, and Ng2 suggested that both HipOPs and CD31(−) HipOPs are hemopoietic stem cell (HSC) niche populations. However, the data support the view that osteoblast differentiation and depletion of VECs modulate the HSC niche. J. Cell. Biochem. 114: 1066–1073, 2013. © 2012 Wiley Periodicals, Inc.
TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells

The extensive applications of cerium (Ce) increased the chance of human exposure to Ce and its compounds. It was reported that Ce was mainly deposited in the bone after administration. However, the potential effect and mechanism of Ce on bone metabolism are not well understood. In this study, we investigated the cellular effects of Ce on the differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms. The results indicated that Ce promoted the osteogenic differentiation and inhibited the adipogenic differentiation of MSCs at cell level. Genes involved in transforming growth factor‐β/bone morphogenetic proteins (TGF‐β/BMP) signaling pathway were significantly changed when the MSCs were exposed to 0.0001 µM Ce by RT2Profiler™ PCR Array analysis. The expression of genes and proteins related to pathways, osteogenic, and adipogenic biomarkers of MSCs upon interaction with Ce was further confirmed by quantitative real‐time reverse transcriptase polymerase chain reaction (Q‐PCR) and Western blot analysis. The results suggest that Ce exerts the effects by interacting with bone morphogenetic protein receptor (BMPR) and activates TGF‐β/BMP signaling pathway, leads to the up‐regulation of the osteogenic master transcription factor, runt‐related transcription factor 2 (Runx 2), and the down‐regulation of the adipocytic master transcription factor, peroxisome proliferator‐activated receptor gamma 2 (PPARγ2). Runx2, which subsequently up‐regulates osteoblast (OB) marker genes collagen I (Col I) and BMP2 at early stages, alkaline phosphatase (ALP), and osteocalcin (OCN) at later stages of differentiation, thus driving MSCs to differentiate into OBs. The results provide novel evidence to elucidate the mechanisms of bone metabolism by Ce. J. Cell. Biochem. 114: 1105–1114, 2013. © 2012 Wiley Periodicals, Inc.
Disorder‐specific versus generic cognitive‐behavioral treatment of anxiety disorders in children and young people: a systematic narrative review of evidence for the effectiveness of disorder‐specific CBT compared with the disorder‐generic treatment, Coping Cat

BACKGROUND Coping Cat (CC), a generic cognitive-behavioral intervention for anxiety disorders in children and young people, is recommended in the United Kingdom for social anxiety disorder (SAD), generalized anxiety disorder, separation anxiety (SA), and specific phobias (SP), with disorder-specific approaches generally favored in treatment of anxiety disorders in adults. OBJECTIVES To compare CC with disorder-specific cognitive-behavioral therapy (CBT) interventions based on anxiety-related treatment outcomes. STUDY SELECTION Primary research articles describing treatment of children and young people aged 7-17 for SAD, generalized anxiety disorder, SA, and SP, using CC or disorder-specific CBT. RESULTS Ten studies implemented CC and four implemented disorder-specific CBT. One study compared CC with a disorder-specific approach. There was a lack of data to support use of CC in treatment of SPs. However, CC was equally effective as disorder-specific treatments for SA and SAD. CONCLUSIONS A lack of data exists for disorder-specific CBT interventions compared to CC.
Effects of metal binding on solubility and resistance of physiological prions depend on tissues and glycotypes

Prion diseases entail the conversion of a normal host‐encoded prion protein (PrPC) into an infectious isoform (PrPSc). Various PrPC types differing in banding profiles and detergent solubility are present in different tissues, but only few PrPSc types have been generated although PrPC acts as substrate. We hypothesize that distinct PrPC subtypes may be converted more efficiently to PrPSc than others. One prerequisite for the analysis is the identification of the PrPC subtypes present in the protein complexes. Metal binding to PrPC is one of the most prominent features of the protein which induces increased proteolysis resistance and structural changes which might play an important role in the conversion process. Here we analyzed the metal‐induced structural PrPC transformation of two different Triton X‐100 soluble PrPC types derived from human platelets and brains by changes in protein solubility. We found that zinc and copper rendered approximately half of total PrPC and mainly un‐ and low‐glycosylated PrPC to the Triton insoluble fraction. Our results indicate the presence of at least two distinct PrPC subtypes by metal interactions. The differentiation of high and low soluble metal bound PrPC offers precious information about PrPC protein composition and provides approaches for analyzing the transformation efficiency to PrPSc. J. Cell. Biochem. 114: 2690–2698, 2013. © 2013 Wiley Periodicals, Inc.
Measuring Mobile Phone Use: Self-Report Versus Log Data

Approximately 40% of mobile phone use studies published in scholarly communication journals base their findings on self-report data about how frequently respondents use their mobile phones. Using a subset of a larger representative sample we examine the validity of this type of self-report data by comparing it to server log data. The self-report data correlate only moderately with the server log data, indicating low criterion validity. The categorical self-report measure asking respondents to estimate “how often” they use their mobile phones fared better than the continuous self-report measure asking them to estimate their mobile phone activity “yesterday. ” A multivariate exploratory analysis further suggests that it may be difficult to identify under- and overreporting using demographic variables alone.
Bremsstrahlung in the gravitational field of a global monopole

We investigate the radiation emitted by a uniformly moving charged scalar particle in the spacetime of a point-like global monopole. We calculate the total energy radiated by the particle and the corresponding spectrum for a small solid angle deficit. We show that the radiated energy is proportional to the cube of the velocity of the particle and to the cube of the Lorentz factor, in the non-relativistic and ultra-relativistic cases, respectively.
Predictors of Use of Social Network Sites at Work - A Specific Type of Cyberloafing

A total of 11,018 employees participated in a survey investigating whether demographic, personality, and work-related variables could explain variance in attitudes towards and actual use of social network sites for personal purposes during working hours. Age was negatively related to both dependent variables. Male gender, single status, and education were positively associated with both dependent variables. Managers had negative attitudes to use, but top-level managers reported more use than other respondents. Access to social network sites at the workplace was positively related to both dependent variables, whereas policies prohibiting showed the opposite relationship. Extraversion and Neuroticism were positively related to both dependent variables. Conscientiousness, positive challenge at work, and quantitative demands were all negatively related to both dependent variables.
When Online Dating Partners Meet Offline: The Effect of Modality Switching on Relational Communication Between Online Daters

Despite the popularity of online dating sites, little is known about what occurs when online dating partners choose to communicate offline. Drawing upon the modality switching perspective, the present study assessed a national sample of online daters to determine whether face-to-face (FtF) relational outcomes could be predicted by the amount of online communication prior to the initial FtF meeting. Results were consistent with the hypothesized curvilinear relationship between the amount of online communication and perceptions of relational messages (intimacy, composure, informality, social orientation), forecasts of the future of the relationship, and information seeking behavior when meeting their partner FtF. The results provide support for the modality switching perspective, and offer important insight for online daters.
THE HYALURONIC ACID RECEPTORS INDUCED BY STRETCH INJURY OF RAT BLADDER IN VIVO AND INFLUENCES SMOOTH MUSCLE CELL CONTRACTION IN VITRO

AbstractPurpose: Loss of bladder compliance from hypercontractility and fibrosis may represent an injury response to excessive intravesical pressure. Together, interactions between cell and extracellular matrix components regulate cell response to injury and extracellular matrix remodeling. The receptor for hyaluronic acid mediated motility (RHAMM) is a recently described hyaluronic acid binding protein known to influence multiple types of cell extracellular matrix interaction in development, injury and cancer. We evaluate the role of RHAMM in mediating early events in bladder stretch injury.Materials and Methods: An acute stretch injury model was used. The rat bladder was injured by hyrodistension inducing gross hematuria. Tissues were analyzed for temporal and spatial expression of RHAMM in the mucosa and detrusor regions by immunostaining, western and reverse transcriptase polymerase chain reaction analyses. The contractile activity of smooth muscle cell primary cultures was analyzed using a gel contra.
Troponin T and troponin I at acut coronary sendrom

Objective: Troponins and tropomyosins are not only the structural proteins that play roles in arrangement of skeletal and heart muscles, but also sensitive and spesific markers of heart muscle damage. In this study we aimed to compare the values of sensitivity, specifity, positive and negative predictive values of troponin T and troponin I which were used to early diagnose of acute coronary syndrome. We also investigated the useability of troponins in diagnoses of the early stage (first 6 hours) of acute myocardial
Standardizing Analysis of Circulating MicroRNA: Clinical and Biological Relevance

Circulating microRNAs (c‐miRNAs) provide a new dimension as clinical biomarkers for disease diagnosis, progression, and response to treatment. However, the discovery of individual miRNAs from biofluids that reliably reflect disease states is in its infancy. The highly variable nature of published studies exemplifies a need to standardize the analysis of miRNA in circulation. Here, we show that differential sample handling of serum leads to inconsistent and incomparable results. We present a standardized method of RNA isolation from serum that eliminates multiple freeze/thaw cycles, provides at least three normalization mechanisms, and can be utilized in studies that compare both archived and prospectively collected samples. It is anticipated that serum processed as described here can be profiled, either globally or on a gene by gene basis, for c‐miRNAs and other non‐coding RNA in the circulation to reveal novel, clinically relevant epigenetic signatures for a wide range of diseases. J. Cell. Biochem. 115: 805–811, 2014. © 2013 Wiley Periodicals, Inc.
Tangential breast irradiation – Optimising the technique

Patients receiving tangential breast irradiation represent a significant proportion of the workload in a radiotherapy department. This paper describes the optimisation of an existing technique for tangential breast irradiation. Simulator sessions have been made more efficient by adopting a systematic approach to the simulation process, and set up times on treatment have been reduced and accuracy increased by improving the reliability of the patient set-up. There has been an overall reduction in both the problems and the time taken to both simulate and treat patients. Patient position variation has been minimised by the use of vac-fix bags and the choice of reference tattoo placement on the patient's skin. The single simulation session for planning treatment has been organised into three parts: definition of target volume, calculation of treatment and field parameters and simulation of treatment fields. The improvement in patient set-up on treatment has been confirmed by a reduction in the variability of FSDs measured during treatment.
Antiadhesive Effect of the Mixed Solution of Hyaluronate and Sodium Carboxymethylcellulose After Strabismus Surgery

Purpose: To evaluate the effect of the mixed solution of hyaluronate and sodium carboxymethylcellulose (HACMC, Guardix-sol) in reducing postoperative adhesion following strabismus surgery. Methods: In 16 rabbits out of 20 rabbits, 3 mm recession of the superior rectus muscle was performed on both eyes and HACMC solution was applied only to one eye which was randomly chosen. The rabbits were divided into a surgery-HACMC group and a surgery-no HACMC group. As a control group, the remaining 4 rabbits had no procedures performed. After 3 weeks, both eyes of 8 rabbits with surgery and 2 rabbits of the control group were enucleated. After staining with hematoxylin-eosin and Masson's trichrome, a histologic examination was performed. In the remaining 10 rabbits, the severity of adhesion in muscle and adjacent tissues was observed by blunt dissection. A researcher blind to the experiments performed all the examinations. Results: A significant reduction in the degree of adhesion (P=0.005) as well as decreased fibrosis (P=0.015) was observed in the surgery-HACMC group. Conclusions: The mixed solution of HACMC was effective in reducing postoperative adhesion after strabismus surgery in rabbits.
Distinguishing Ventricular Arrhythmia Originating from the Right Coronary Cusp, Peripulmonic Valve Area, and the Right Ventricular Outflow Tract: Utility of Lead I

Outflow tract ventricular arrhythmia (OTVA) can be complicated to target for ablation when originating from either the periaortic or pulmonary valve (PV) region. Both sites may present with a small R wave in lead V1. However, the utility of lead I in distinguishing these arrhythmia locations is unknown.
SMAD3 and SP1/SP3 Transcription Factors Collaborate to Regulate Connective Tissue Growth Factor Gene Expression in Myoblasts in Response to Transforming Growth Factor β

Fibrotic disorders are characterized by an increase in extracellular matrix protein expression and deposition, Duchene Muscular Dystrophy being one of them. Among the factors that induce fibrosis are Transforming Growth Factor type β (TGF‐β) and the matricellular protein Connective Tissue Growth Factor (CTGF/CCN2), the latter being a target of the TGF‐β/SMAD signaling pathway and is the responsible for the profibrotic effects of TGF‐β. Both CTGF and TGF are increased in tissues affected by fibrosis but little is known about the regulation of the expression of CTGF mediated by TGF‐β in muscle cells. By using luciferase reporter assays, site directed mutagenesis and specific inhibitors in C2C12 cells; we described a novel SMAD Binding Element (SBE) located in the 5′ UTR region of the CTGF gene important for the TGF‐β‐mediated expression of CTGF in myoblasts. In addition, our results suggest that additional transcription factor binding sites (TFBS) present in the 5' UTR of the CTGF gene are important for this expression and that SP1/SP3 factors are involved in TGF‐β‐mediated CTGF expression. J. Cell. Biochem. 116: 1880–1887, 2015. © 2015 Wiley Periodicals, Inc.
Screening of nutritional status of male undergraduates in Kolkata using anthropometric indices

Context and Aims: Anthropometric measurements can easily reflect the nutritional status of an individual. The purpose of this study is to investigate the magnitude of obesity and undernutrition based on body mass index (BMI) and direct, derived anthropometric variables, among college students of Kolkata. Subjects and Methods: Anthropometry is generally considered as the single most easily obtainable, inexpensive, and non-invasive method that reflects body composition. A total of 100 male students aged 18-22 years of different undergraduate colleges of Kolkata have participated in this study. Anthropometric profiles (a total of 24 direct and derived variables) including weight, height, waist, and hip circumferences were measured. Results: Results showed 14.68% of the studied population are underweight, 9.25% are overweight, and 0.89% is obese, as per BMI. The present report showed higher prevalence of normal BMI (75.16%) in male college students, whereas obesity was almost found to absent among participants. Conclusion: The findings of the present report indicated occurrence of almost normal nutritional status among male undergraduates of Kolkata based on BMI and anthropometric indices
Global Gene Deregulations in FASN Silenced Retinoblastoma Cancer Cells: Molecular and Clinico‐Pathological Correlations

Activation of fatty acid synthase (FASN) enzyme in the de novo lipogenic pathway has been reported in various cancers including retinoblastoma (RB), a pediatric ocular cancer. The present study investigates lipogenesis‐dependent survival of RB cancer cells and the associated molecular pathways in FASN silenced RB cells. The siRNA‐mediated FASN gene knockdown in RB cancer cells (Y79, WERI RB1) repressed FASN mRNA and protein expressions, and decreased cancer cell viability. Global gene expression microarray analysis was performed in optimized FASN siRNA transfected and untransfected RB cells. Deregulation of various downstream cell signaling pathways such as EGFR (n = 55 genes), TGF‐beta (n = 45 genes), cell cycle (n = 41 genes), MAPK (n = 39 genes), lipid metabolism (n = 23 genes), apoptosis (n = 21 genes), GPCR signaling (n = 21 genes), and oxidative phosporylation (n = 18 genes) were observed. The qRT‐PCR validation in FASN knockdown RB cells revealed up‐regulation of ANXA1, DAPK2, and down‐regulation of SKP2, SREBP1c, RXRA, ACACB, FASN, HMGCR, USP2a genes that favored the anti‐cancer effect of lipogenic inhibition in RB. The expression of these genes in primary RB tumor tissues were correlated with FASN expression, based on their clinico‐pathological features. The differential phosphorylation status of the various PI3K/AKT pathway proteins (by western analysis) indicated that the FASN gene silencing indeed mediated apoptosis in RB cells through the PI3K/AKT pathway. Scratch assay clearly revealed that FASN silencing reduced the invading property of RB cancer cells. Dependence of RB cancer cells on lipid metabolism for survival and progression is implicated. Thus targeting FASN is a promising strategy in RB therapy. J. Cell. Biochem. 116: 2676–2694, 2015. © 2015 Wiley Periodicals, Inc.
Bussulfano e melfalano como regime de condicionamento para o transplante autogênico de células-tronco hematopoéticas na leucemia mielóide aguda em primeira remissão completa

Twenty-two consecutive patients with acute myeloid leukemia in first complete remission submitted to autologous hematopoietic stem cells transplantation conditioned with busulfan and melphalan were evaluated between 1993 and 2006. The overall survival, according to the Kaplan-Meier curve, was 57.5% at 36 months, with a "plateau" at 20 months after transplant. Factors such as gender, French-American-British (FAB) classification of acute myeloid leukemia, induction therapy, intensive consolidation, remission after the first cycle of induction and source of cells had no impact on survival. One patient with poor prognosis before the procedure died a year after transplantation. Nine patients died, eight by relapse and one because of bleeding. Death before 100 days occurred for two patients, one due to relapse and the other bleeding caused by refractory thrombocytopenia related to the procedure. In conclusion, the conditioning regiment with busulfan and melphalan is a valid option compared to the other conditioning regimens, with an excellent overall survival.
Multiple Levels of Regulation of Sororin by Cdk1 and Aurora B

The cohesin complex holds sister chromatids together until all chromosomes are properly attached to the mitotic spindle. Cleavage of the cohesin subunit Rad21 at the metaphase to anaphase transition allows separation of sister chromatids and is fundamental for the creation of identical daughter cells. Sororin blocks removal of cohesin from chromosomes from S phase until mitosis. In mitosis, Sororin is phosphorylated by Cdk1 releasing it from the cohesin complex. Aurora B phosphorylation of Sororin may play a similar role as Cdk1. Using PhosTag electrophoresis, we detect multiple Sororin species suggesting that phosphorylation of Sororin in mitosis is heterogeneous. Mutating the Cdk1 consensus site S21 to alanine eliminates many of the phosphorylated species suggesting that S21 is a key site of phosphorylation in vivo. Inhibiting Aurora B reduces phosphorylation of Sororin in cells, but only if Cdk1 sites are intact suggesting that some phosphorylation events on Sororin may be sequential. Surprisingly, mutating Aurora B consensus sites in Sororin causes it to relocalize to the nucleolus during interphase and blocks its interaction with chromosomes in Aurora B‐inhibited cells. These observations indicate that phosphorylation plays unexpected roles in regulating the subcellular localization of Sororin. J. Cell. Biochem. 117: 351–360, 2016. © 2015 Wiley Periodicals, Inc.
A randomized, controlled trial of two-thumb vs two-finger chest compression in a swine infant model of cardiac arrest

Background. The American Heart Association (AHA) currently recommends two-finger (TF) chest compression for infants. A previous study demonstrated that two-thumb (TT) with lateral chest wall compression provided significantly higher arterial pressures than did the TF method. Limitations of that study included the lack of an asphyxial model and nonstandardized compression forces.objective. To test the hypothesis that TT chest compression generates higher arterial pressures than does the TF method, using an asphyxial model. Also, by standardizing sternal compression force (SCF), the authors sought to show that the increased pressures are the result of thoracic compression.Method. The study was a randomized, crossover trial in immature swine weighing 10 kg. Each swine was sedated, anesthetized, paralyzed, intubated, and mechanically ventilated on room air. A femoral arterial catheter was placed. Cardiac arrest was induced by asphyxiation and verified by ECG and pressure tracings. Eleven AHA-certified basic r.