Miracle-dz/UniFAR-FTUs huggingface dataset:
COVID-19 Transmission in a Psychiatric Long-Term Care Rehabilitation Facility: An Observational Study.

Objective To report the clinical characteristics and transmission rate of coronavirus disease 2019 (COVID-19) in a community inpatient long-term care psychiatric rehabilitation facility designed for persons with serious mental illness to provide insight into transmission and symptom patterns and emerging testing protocols, as well as medical complications and prognosis. Methods This study examined a cohort of 54 residents of a long-term care psychiatric rehabilitation program from March to April 2020. Baseline demographics, clinical diagnoses, and vital signs were examined to look for statistical differences between positive versus negative severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) groups. During the early phase of the pandemic, the facility closely followed the local shelter-in-place order (starting March 19, 2020) and symptom-based testing. Results Of the residents, the primary psychiatric diagnoses were schizoaffective disorder: 28 (51.9%), schizophrenia: 21 (38.9%), bipolar I disorder: 3 (5.5%), and unspecified psychotic disorder: 2 (3.7%). Forty (74%) of 54 residents tested positive for SARS-COV-2, with a doubling time of 3.9 days. There were no statistical differences between the positive SARS-COV-2 versus negative groups for age or race/ethnicity. Psychiatric and medical conditions were not significantly associated with contracting SARS-COV-2, with the exception of obesity (n = 17 [43%] positive vs n = 12 [86%] negative, P = .01). Medical monitoring of vital signs and symptoms did not lead to earlier detection. All of the residents completely recovered, with the last resident no longer showing any symptoms 24 days from the index case. Conclusion Research is needed to determine optimal strategies for long-term care mental health settings that incorporate frequent testing and personal protective equipment use to prevent rapid transmission of SARS-COV-2.
CORR Insights®: Is Use of BMP-2 Associated with Tumor Growth and Osteoblastic Differentiation in Murine Models of Osteosarcoma?

Unfortunately, we have made limited progress in adequately addressing questions regarding the safe use of recombinant human bone morphogenetic protein 2 (rhBMP-2) in patients undergoing limb salvage. In the current study, Kendal et al. [3] confirmed what others have found [7, 10] during a period in recent history that has included the sequencing of genomes (human, canine, murine, and others), the further refinement of recombinant DNA technology, and extensive preparation and marketing of recombinant BMPs. During this time, we have also seen the evolution of investigational systems, and quickly thereafter, the streamlining, development, and production of elaborate,market-ready systems for the largescale study of gene expression [4, 5, 11, 12]. However, one thing has remained constant: surgeons’ and researchers’ concerns about the substantial risks associated with the use of rhBMP-2 in patients with osteosarcoma [7, 10]. Prior studies [7, 9, 10] and the current one [3] have convinced me that limbsalvage surgery for patients with osteosarcoma is not a safe clinical environment for the evaluation of new clinical applications for the known osteogenic properties of rhBMP-2. The most important findings of this study [3] are the documentation of the proliferative effect of BMP-2 signaling on the biologic behavior of osteosarcoma, particularly the proliferative effects noted in vivo. The authors implemented a novel use for microCT imaging, previously used to demonstrate the beneficial biologic effects of BMP-2 signaling in fracture healing in rodent in vivo models [12], to quantify the burden of osteosarcomas inmodels evaluated in the current study. The authors emphasize that we should temper our enthusiasm for the use of BMP-2 in patients with osteosarcoma. To use the authors’ words, “its clinical use should remain limited in an effort to do no harm” [3].
Oral administration of potato peel extract affects serum blood metabolites, liver function and ameliorating oxidative stress induced in rabbits exposed to cold stress

Abstract This study investigated the effect of potato peel extract (PPE), orally administrated to rabbits, on serum blood metabolites and ameliorating oxidative stress induced by cold stress under Egyptian winter conditions. Twenty-four bucks grouped into three treatments (8 animals per group) were used for the experiment. The animals received 1.5 ml of water orally, containing 0 (PPE0), 25 (PPE25) or 50 (PPE50) mg PPE/kg live weight. Bucks were randomly assigned into three homogenous equal groups according to the level of PPE. Treatments were applied to each animal every two days over a period of three months including one month as an adaptation period. At the 8th week of the experiment, blood samples were collected from each buck and at the end of the experiment, bucks were slaughtered, and some organs were collected and weighed. The PPE improved (p < 0.05) blood total protein, albumin, globulin and glucose. The blood concentration of total lipid, cholesterol, triglyceride, low density lipoprotein and very low-density lipoprotein (were increased (p < 0.02) in PPE rabbits. Furthermore, PPE extract doses decreased (p < 0.001) oxidant thiobarbituric reactive substance (TBARS) in both blood and liver. Other liver and blood antioxidant system enzymes such as catalase, glutathione peroxidase, and superoxide dismutase were improved (p < 0.005) by PPE supplementation. Overall, oral administration of PPE up to 50 mg/kg live weight can have positive effects on rabbit health under cold stress.
Potential Anthropogenic Pollution of High-technology Metals with a Focus on Rare Earth Elements in Environmental Water

In recent years, the utilization of high-technology metals such as rare earth elements (REEs), which exist in extremely low quantities in the Earth, has rapidly increased with the development of new types of industrial materials and pharmaceutical products. This review provides an overview of a new type of potential anthropogenic pollution caused by high-technology metals, with a focus on REEs released into environmental waters from waste treatment plants. In this paper, potential anthropogenic pollution was defined as pollution caused by metals gradually enriched in the environment by human activity, although standard and guideline concentrations of these elements are not regulated by environmental quality standards for water pollution. We review the analytical methods of REEs and the potential anthropogenic pollution of REEs with a focus on Gd, from the viewpoints of a comparison of the degree of Gd anomaly, chemical speciation, ecotoxicology, and bioaccessibility. Moreover, we also highlight the comprehensive analysis based on multielement analysis of high-technology metals as well as REEs for the further screening for potential anthropogenic pollution.
Age‐Specific Effects of Lexical–Semantic Networks on Word Production

Abstract The lexical–semantic organization of the mental lexicon is bound to change across the lifespan. Nevertheless, the effects of lexical–semantic factors on word processing are usually based on studies enrolling young adult cohorts. The current study aims to investigate to what extent age‐specific semantic organization predicts performance in referential word production over the lifespan, from school‐age children to older adults. In Study 1, we conducted a free semantic association task with participants from six age‐groups (ranging from 10 to 80 years old) to compute measures that capture age‐specific properties of the mental lexicon across the lifespan. These measures relate to lifespan changes in the Available Richness of the mental lexicon and in the lexical–semantic Network Prototypicality of concrete words. In Study 2, we used the collected data to predict performance in a picture‐naming task on a new group of participants within the same age‐groups as for Study 1. The results show that age‐specific semantic Available Richness and Network Prototypicality affect word production speed while the semantic variables collected only in young adults do not. A richer and more prototypical semantic network across subjects from a given age‐group is associated with faster word production speed. The current results indicate that age‐specific semantic organization is crucial to predict lexical–semantic behaviors across the lifespan. Similarly, these results also provide cues to the understanding of the lexical–semantic properties of the mental lexicon and to lexical selection in referential tasks.
Sémont Maneuver for Benign Paroxysmal Positional Vertigo Treatment: Moving in the Correct Plane Matters

Hypothesis: We aimed to investigate the effect of the head excursion angle on the success of the Sémont Maneuver (SM). Background: SM is performed with the head turned by 45 degrees toward the unaffected ear. In clinical routine, it is unlikely that physicians can turn the head to a position of exactly 45 degrees. Moreover, it is unclear how possible deviations from 45 degrees would affect the outcome with SM. Methods: We used an in vitro model (upscaled by ×5) of a posterior semicircular canal with canalithiasis to study head excursion angles (0–75 degrees) and minimum waiting times in SM. Additionally, we measured actual head excursion angles performed by trained physicians during SM on a healthy subject. Results: Successful canalith repositioning to the utricle was possible at head excursion angles between 21 and 67 degrees. Waiting time increased from 16 to 30 seconds with increasing deviation from 45 degrees. Angles larger than 67 degrees or smaller than 21 degrees did not lead to successful repositioning even after a waiting period of 5 minutes. Physicians set head excursion angles of 50 degrees ±SD 4.8 degrees while performing the SM. Conclusion: Angular deviations up to ±20 degrees from the ideal SCC plane (45 degrees) still allows for successful SM. Although the tested physicians tended to underestimate the actual head excursion angle by 5 degrees (and more), the success of SM will not be affected provided that the waiting time is sufficiently long. Further, the results suggest that the Brandt-Daroff maneuver is a form of habituation training rather than a liberatory maneuver.
The influence of web-based questionnaire presentation variations on survey cooperation and perceptions of survey quality

The Internet can be an excellent medium for many survey research applications. Webbased survey systems can administer and process large numbers of surveys typically for a substantially lower cost than traditional survey modes. This survey method is becoming increasingly popular, yet the empirical research to guide web survey designers is still young. Understanding what motivates potential respondents to cooperate with a request for online survey participation is one of the areas of research that will inform web-based questionnaire design principles and guide the practice of creating and administering these surveys. Research in this area is relatively new compared to the decades of research on gaining cooperation for more traditional survey modes. This study is intended to contribute to the emerging empirically-based theory about web-based survey respondents’ behavior. We look at factors that have been found to influence response rates in traditional survey modes and examine their effect in the web-based mode. We examine use of color and graphics, various item response option formats, government sponsorship identification and suggested time needed to complete the survey as possibly influential characteristics for online survey cooperation. The respondents’ perceptions of the survey’s quality are also compared across these variable conditions.
Detection of Mycoplasma wenyonii and “Candidatus Mycoplasma haemobos” from Japanese Black breeding cows in Kyushu and Okinawa region, southern part of Japan

This study aimed to elucidate the epidemiological status of hemoplasma infection and investigate the interaction between Theileria orientalis and hemoplasmas in Japanese Black breeding cows raised in the Kyushu and Okinawa regions. Blood samples were collected from 400 cows from 80 different farms in eight prefectures (five samples per farm and 10 farms per prefecture). Mycoplasma wenyonii (Mw), “Candidatus Mycoplasma haemobos” (CMh), and T. orientalis were examined by polymerase chain reaction (PCR) assay using whole blood samples. PCR results showed that 91.5% (366/400) of cows were positive for bovine hemoplasma: 40.3% were infected with Mw only, 9.5% with CMh only, and 41.8% with both species. T. orientalis was detected in 36% (144/400) of cows. The infection rate of T. orientalis was higher in the grazing group (P<0.001) than in the housed group, while the rate of CMh infection was higher (P<0.05) in the housed group than in the grazing group, suggesting that not only the tick but also other arthropod vectors may contribute to hemoplasma transmission. Although the cows with hemoplasma dual infection showed higher (P<0.05) white blood cell counts compared with hemoplasma-negative cows, there was no difference in hematologic parameters related to the anemia between the hemoplasma-positive and -negative animals. This may indicate that Japanese Black cattle could have resistance to the anemia caused by infection with hemoplasma.
Implementing standards of care for heart failure patients in general practice – the IMPACT-B study protocol

Abstract Background Heart failure (HF) is an important health problem. Most chronic HF management occurs in primary care. Although guidelines exist, there is an important implementation gap in current HF care in Belgium. Methods We will conduct a non-randomised, non-controlled prospective observational trial to implement guideline-recommended disease management interventions in primary care in Leuven, a region of ±100.000 inhabitants. These interventions include education of general practitioners, reimbursement of the analysis of circulating natriuretic peptides and audits in the electronic health record (EHR), training and implementation of HF educators in primary care, and a protocol to structure transition to primary care after discharge. The main objective is to study and implement interventions in an iterative implementation process. Conclusions We will evaluate the implementation of several guideline-recommended disease management interventions to optimise the diagnosis and treatment of heart failure in a real-world primary care setting. Trial registration NCT04334447 (clinicaltrials.gov)
lncRNA SNHG14 involved in trophoblast cell proliferation, migration, invasion and epithelial–mesenchymal transition by targeting miR-330-5p in preeclampsia

Summary Preeclampsia (PE), a pregnancy-specific disease, has become one of the leading causes of maternal and neonatal morbidity and mortality. Pathogenesis of PE has still not been fully addressed and there is a great need to develop early diagnosis markers and effective therapy. This study aimed to determine if lncRNA SNHG14 has a protective effect on placental trophoblast and prevents PE. SNHG14 levels in the peripheral blood from patients with PE or from women with healthy pregnancies were detected using RT-qPCR. The relationship between SNHG14 and miR-330-5p was determined using a dual-luciferase reporter assay. In addition, cell proliferation and cell cycle were evaluated by performing CCK8 assays and flow-cytometric analysis, respectively. Wound-healing and transwell assays were performed to assess cell migration and invasion ability. lncRNA SNHG14 was downregulated in PE patients; it was involved in trophoblast proliferation and regulated cell proliferation during G1/S transition. In addition, lncRNA SNHG14 promoted migration, invasion and epithelial–mesenchymal transition (EMT) in HTR-8/SVneo cells. Luciferase reporter assay indicated that lncRNA SNHG14 served as a molecular sponge for miR-330-5p and negatively regulated miR-330-5p expression in PE. Furthermore, the effects of silenced SNHG14 on trophoblast proliferation, migration, invasion and EMT were reversed by addition of miR-330-5p inhibitor, suggesting that in PE lncRNA SNHG14 functions by competitively binding to miR-330-5p. Taken together, the current study demonstrated that in PE lncRNA SNHG14 is a vital regulator by binding to miR-330-5p. SNHG14 might serve as a therapeutic application in PE progression.
Effects of miR-146a-5p on chondrocyte interleukin-1β-induced inflammation and apoptosis involving thioredoxin interacting protein regulation

Objective Osteoarthritis (OA) is a chronic degenerative arthropathy characterized by articular cartilage degeneration, subchondral osteosclerosis, and hyperosteogeny. MicroRNAs (miRNAs) play an important regulatory role in its pathological development, so this study explored the effect and potential mechanism of miR-146a-5p in interleukin (IL)-1β-induced OA cartilage injury. Methods The human chondrosarcoma cell line SW1353 and normal human chondrocytes C28/I2 were stimulated by IL-1β to construct the OA chondrocyte model. miR-146a-5p and thioredoxin interacting protein (TXNIP) expression levels were detected by quantitative real-time (qRT)-PCR and western blot. Their expression was modified by transfecting an miR-146a-5p inhibitor, mimic, and pcDNA-TXNIP. The relationship between miR-146a-5p and TXNIP was analyzed by the dual-luciferase assay, while cell viability, apoptosis, and inflammatory expression were determined by cell counting, TUNEL staining, and ELISA, respectively. Results miR-146a-5p expression was upregulated in SW1353 and C28/I2 cells stimulated by IL-1β. miR-146a-5p knockdown significantly enhanced cell activity, inhibited inflammatory factor expression, and reduced cell apoptosis. The dual-luciferase assay revealed TXNIP as a target gene of miR-146a-5p and suggested that miR-146a-5p promotion of OA damage could be reversed by upregulating TXNIP. Conclusion These results suggest that miR-146a-5p inhibits cell proliferation and promotes apoptosis and the inflammatory response in OA cartilage injury by modulating TXNIP.
Arabidopsis ORANGE protein regulates plastid preprotein import through interacting with Tic proteins.

Chloroplast targeted proteins are actively imported into chloroplasts via the machinery spanning the double layered membranes of chloroplasts. While the key translocons at the outer (TOC) and inner (TIC) membranes of chloroplasts are defined, proteins that interact with the core components to facilitate preprotein import are continuously being discovered. A DnaJ-like chaperone ORANGE (OR) protein is known to regulate carotenoid biosynthesis as well as plastid biogenesis and development. In this study, we found that OR physically interacts with several Tic proteins including Tic20, Tic40, and Tic110 in the classic TIC core complex of the chloroplast import machinery. Knocking out or and its homologous gene or-like greatly affects the import efficiency of some photosynthetic and non-photosynthetic preproteins. Consistent with the direct interactions of OR with Tic proteins, the binding efficiency assay revealed that the effect of OR likely occurs at translocation at the inner envelope membrane (i.e. at the TIC complex). OR is able to reduce Tic40 protein turnover rate through its chaperone activity. Moreover, OR was found to interfere with the interaction between Tic40 and Tic110, and reduces the binding of preproteins to Tic110 in aiding their release for translocation and processing. Our findings suggest that OR plays a new and regulatory role in stabilizing key translocons and in facilitating the late stage of plastid preprotein translocation to regulate plastid preprotein import.
Evaluation of the neuroprotective efficiency of sodium hydrosulfide in neonatal rats with the induced hypoxic-ischemic encephalopathy model.

AIMS Hypoxic ischemic encephalopathy is one of the main causes of neonatal deaths. The objective of this study was to evaluate the neuroprotective effect of antioxidant and anti-inflammatory properties of sodium hydrosulfide (NaHS) in neonatal rats with hypoxic ischemic encephalopathy, as well as its effect on neuronal apoptosis through histopathological and biochemical tests. METHODS Forty-seven-day‑old rats with induced hypoxia‑ischemia (HI) were randomly separated into four groups. Half an hour after the induction of hypoxic-ischemia, serum physiological (SF), 50 µmol/kg NaHS, or 100 µmol/kg NaHS were intraperitoneally given to the rats. RESULTS Apoptotic cells in the brain tissue of rats in HI + NaHS 50 μmol/kg, and HI + NaHS 100 μmol/kg groups decreased compared to HI group (p = 0.00). While HI + NaHS 50 μmol/kg and HI + NaHS 100 μmol/kg groups yielded no difference in TNF-α, IL-6, and iNOS levels as compared to the HI group, an increase in NGF was detected in the 50 µmol/kg and 100 µmol/kg NaHS groups (p = 0.34, p = 0.24, p = 0.26, p = 0.026, p = 0.017). When TOS, TAS and OSI levels were compared, an increase in TAS and OSI and a decrease in TOS were observed in the treatment groups as compared to HI group. CONCLUSIONS NaHS given to hypoxic-ischemic encephalopathy model significantly decreased apoptosis in neurons and had a neuroprotective efficacy with an increase in NGF levels (Tab. 1, Fig. 3, Ref. 25).
Pretransplantation splenomegaly frequently persists after liver transplantation and can manifest as hypersplenism and graft fibrosis ‐ a retrospective study

The risk factors and clinical impact of post‐transplantation splenomegaly (SM) are poorly understood. We investigated the predictors and impacts of post‐transplantation SM in 415 LT patients at Kyoto University Hospital from April 2006 to December 2015. First, the predictors and clinical consequences of SM three years post‐transplantation were analyzed among spleen‐preserved recipients. Second, the clinical data of surviving recipients three years post‐transplantation were compared between splenectomized and spleen‐preserved recipients. There was no difference in indication for liver transplantation between these two groups. Third, survival outcomes were compared between splenectomized and spleen‐preserved recipients. SM was determined as a SV/body surface area (BSA) higher than 152 ml/m2. In the first analysis, preoperative SM occurred in 79.9% recipients and SM persisted three years post‐transplantation in 72.6% recipients among them. Preoperative SV/BSA was the only independent predictor of three year post‐transplantation SM, which was associated with lower platelet (PLT), white blood cell (WBC) counts and significant graft fibrosis (21.4% vs. 2.8%). In the second analysis, spleen‐preservation was related to lower PLT, WBC counts and a higher proportion of significant graft fibrosis (26.7% vs. 7.1%) three years post‐transplantation. In the third analysis, spleen‐preserved recipients showed worse survival than splenectomized recipients. In conclusion, preoperative SM frequently persists more than three years post‐transplantation and is associated with subclinical hypersplenism, graft fibrosis, graft loss, and even death.