Miracle-dz/UniFAR-FTUs huggingface dataset:
IMPORTANCE OF DATA STRUCTURE IN STATISTICAL ANALYSIS OF DENDRITIC SPINE MORPHOLOGY.

: Background: Dendritic spine morphology is heterogeneous and highly dynamic. To study the changing or aberrant morphology in test setups, often spines from several neurons from a few experimental units e.g. mice or primary neuronal cultures are measured. This strategy results in a multilevel data structure, which, when not properly addressed, has a high risk of producing false positive and false negative findings. Methods: We used mixed-effects models to deal with data with a multilevel data structure and compared this method to analyses at each level. We apply these statistical tests to a dataset of dendritic spine morphology parameters to illustrate advantages of multilevel mixed-effects model, and disadvantages of other models.
Effects of synthetic LH-RH and analog on plasma gonadotropin levels and maturational response to 17 alpha Hydroxy - 20 beta - dihydroprogesterone

Breton, B. , Jalabert, B. , Bieniarz, K. , Sokolovska, M. and Epler, P., 1983. Effects of synthetic LH-RH and analog on plasma gonadotropin levels and maturational response to 17cu-hydroxy-20p-dihydroprogesterone. Aquaculture, 32: 105-114. The effects of LH-RH and analog on the stimulation of gonadotropin (GtH) secretion have been studied in order to use them as priming, before inducing maturation with 17cu-hydroxy-20p-dihydroprogesterone (17a-20pP) in the carp Cyprinus carpio. One injection of LH-RH (2 Mg/kg body weight) or LH-RH analog (0.3 @g/kg) induces a transient rise in the plasma GtH concentration, but subsequent 17a-20~P treatment does not result in an increase in the number of oocytes with germinal vesicle breakdown or in ovulation. Two and three in,ections of LH-RH or analog at the same dosages are followed by a stimulation of GtH secretion (to 30 ng/ml) which is maintained for 6-9
Genome-wide association study in two cohorts from a multi-generational mouse 1 advanced intercross line highlights the difficulty of replication 2 3

31 Replication is considered to be critical for genome-wide association studies (GWAS) in 32 humans, but is not routinely performed in model organisms. We explored replication using an 33 advanced intercross line (AIL) which is the simplest possible multigenerational intercross. We 34 re-genotyped a previously published cohort of LG/J x SM/J AIL mice (F34; n=428) using a 35 denser marker set and also genotyped a novel cohort of AIL mice (F39-43; n=600) for the first 36 time. We identified 110 significant loci in the F34 cohort, 36 of which were new discoveries 37 attributable to the denser marker set; we also identified 27 novel significant loci in the F39-43 38 cohort. For traits measured in both cohorts (locomotor activity, body weight, and coat color), the 39 genetic correlations were high, although, the F39-43 cohort showed systematically lower SNP40 heritability estimates. We then attempted to replicate loci identified in either F34 or F39-43 in the 41 other cohort. Albino coat color was robustly replicated; we observed only partial replication of 42 associations for locomotor activity and body weight. Finally, we performed a mega-analysis of 43 locomotor activity and body weight by combining F34 and F39-43 cohorts (n=1,028), which 44 identified four novel loci. The incomplete replication was inconsistent with simulations we 45 performed to estimate our power to replicate. This may reflect: 1) false positives errors in the 46 discovery cohort, 2) environmental or genetic heterogeneity between the two samples, or 3) the 47 systematic over estimation of the effect sizes at significant loci (“Winner’s Curse”). Our results 48 demonstrate that it is difficult to replicate GWAS results even when using similarly sized 49 discovery and replication cohorts drawn from the same population. 50 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/387613 doi: bioRxiv preprint
Propofol inhibits stromatoxin-1-sensitive voltage-dependent K[sup]+[sup] channels in pancreatic β-cells and enhances insulin secretion

Background: Proper glycemic control is an important goal of critical care medicine, including perioperative patient care that can influence patients’ prognosis. Insulin secretion from pancreatic β-cells is generally assumed to play a critical role in glycemic control in response to an elevated blood glucose concentration. Many animal and human studies have demonstrated that perioperative drugs, including volatile anesthetics, have an impact on glucose-stimulated insulin secretion (GSIS). However, the effects of the intravenous anesthetic propofol on glucose metabolism and insulin sensitivity are largely unknown at present.
Optimal linear generator with Halbach array for harvesting of vibration energy during human walking

In IT business, the capacity of the battery in smartphone was drastically improved to digest various functions such as communication, Internet, e-banking, and entertainment. Although the capacity of the battery is improved, it still needs to be upgraded due to customer’s demands. In this article, we optimize the design of the linear generator with the Halbach array to improve the efficiency of harvesting vibration energy during human walking for the battery capacitance. We propose the optimal design of the tubular permanent magnet with the linear generator that uses a Halbach array. The approximate model is established using generic algorithm. Furthermore, we performed electromagnetic finite element analysis to predict the induced voltage.
Flight Performance of Fixed and Rotary Wing Aircraft A. Filippone Elsevier Butterworth-Heinemann, Linacre House, Jordan Hill, Oxford OX2 8DP, UK. 2006. 565pp. £39.99. ISBN 0-7506-6817-2.

ters 9 to 11, and the book’s final chapter is dedicated solely to the MATLAB programmes accompanying the text. A useful addition to the software is the inclusion of a nonlinear simulation of the F-16, which allows the reader to generate data and perform the subsequent analysis using SIDPAC. This is an excellent book which achieves its goal of providing a detailed account of both the theoretical and practical aspects of aircraft system identification. The authors’ arguments are well illustrated by case studies and practical examples from their research experiences. The availability of the SIDPAC toolset and flight test data is crucial to the text because, as stressed throughout the book, a complete understanding of system identification can only be achieved through practical experience. In comparison to the two other textbooks available on aircraft system identification (Aircraft and Rotorcraft System Identification: Engineering Methods with Flight Test Examples by Mark B. Tischler and Robert K. Remple, and Flight Vehicle System Identification: A Time Domain Methodology by Ravindra V. Jategaonkar) the mathematical derivations in this publication begin at the most fundamental level and it is also the only one of the three to cover parameter estimation in both the time and the frequency domain. For these reasons, this book is probably the most suitable for readers completely new to the subject. At the same time, there is much in this text that will interest practicing engineers already engaged in the field. Stephen Carnduff, Affiliate
Material Selection for Fuselage Using Integrated SWARA-MULTIMOORA Approach

The variety of engineering materials is increasing day by day with alloying, post-treatments such as heat treatment, and the production of composite materials, besides pure elements. The selection of the most suitable material for each different part to be produced from such a large material pool is very important. In this study, which aims to select materials for the fuselage of the passenger aircraft, firstly criteria and alternatives have been determined in line with expert opinions. The criteria weights were obtained using SWARA method, and then alternative materials were ranked by MULTIMOORA method according to the criteria weights. Abstract The variety of engineering materials is increasing day by day with alloying, post-treatments such as heat treatment, and the production of composite materials, besides pure elements. The selection of the most suitable material for each different part to be produced from such a large material pool is very important. The selection of suitable material for the fuselage, which constitutes an important part of the aircraft, is important in terms of lightness and cost, as well as thermal and mechanical properties. In this study, which aims to select materials for the fuselage of the passenger aircraft, firstly criteria and alternatives have been determined in line with expert opinions. In the study in which criteria such as density, tensile strength, shear strength and cost were taken into consideration, the criteria weights were obtained using SWARA method, and then alternative materials were ranked by MULTIMOORA method according to the criteria weights. As a result, three most suitable materials for the fuselage of the passenger aircraft were determined as CFRP, Ti-6Al-4V and GFRP, respectively.
Synergistic Induction of Osteocalcin Gene Expression IDENTIFICATION OF A BIPARTITE ELEMENT CONFERRING FIBROBLAST GROWTH FACTOR 2 AND CYCLIC AMP RESPONSIVENESS IN THE RAT OSTEOCALCIN PROMOTER*

Fibroblast growth factors (FGFs) are important regu- lators of calvarial osteoblast growth and differentiation. We have studied the regulation of the osteoblast-specific gene osteocalcin (OC) by FGF2 in phenotypically immature MC3T3-E1 calvarial osteoblastic cells. FGF2 mark- edly induces OC mRNA accumulation in MC3T3-E1 cells in the presence of forskolin (FSK). Similarly, OC pro- moter activity (luciferase reporter) is up-regulated 6–10-fold by FGF2/FSK or by FGF2/8-bromo cyclic AMP. Half-maximal induction of OC promoter activity occurs at 1 n M FGF2. By 5 (cid:42) deletion analysis and dinucleotide point mutations, we map one component of this FGF2/ FSK response to a GCAGTCA motif in the region (cid:50) 144 to (cid:50) 138 relative to the OC transcription initiation site. The OC promoter region (cid:50) 154 to (cid:50) 90 confers FGF2/FSK responsiveness on the Rous sarcoma virus minimal pro- moter. By 3 (cid:42) and internal deletion analyses, the region between (cid:50) 90 to (cid:50) 99 is also found to be necessary for FGF2/FSK synergy (encodes a PuGGTCA motif previ- ously identified as a component of FSK induction). A DNA binding activity that recognizes the region (cid:50)
Airmass Transformation over the Semiarid Region around North China anc Abrupt Change in the Structure of the Baiu Front in Early Summer By Kuranoshin Kato

According to the observation data from 1975 to 1979, the "structure change" in the Baiu front in China occurs in late May in each year: the vanishment of the horizontal temperature gradient in the lower layer and the change in the stability for moist convection from stable state to nearly neutral state. The ground surface temperature in North China increases rapidly and its horizontal gradient along *115*E vanishes in China around May in these years. In late May of 1979 the air temperature in the lower layer rises largely in the semiarid region (Takla Makan*Mongol Plateau*Gobi Desert*North China) and the polar frontal zone in the eastern part of Eurasia (90*E*140*E) shifts from *45*N to *60*N. The area where the deep mixed layer due to dry convection with a thickness of more than 200mb (2000m) begins to develop rapidly in late April over the semiarid region from Takla Makan to North China, a month before the "structure change" in the Baiu front in China. The heat budget in the atmosphere over Mongol Plateau suggests that the air temperature rise and the maintenance of high temperature in the lower layer in May are mainly due to the sensible heat supplied from the ground as well as in North China. The intense airmass transformation over that region warms the air parcel to some extent before it reaches North China. This seems to provide the more favorable condition for the change in the thermal structure in the Baiu front in South China. The "structure change" in the Baiu front in China and the northward shift of the polar front in the eastern part of Eurasia seems to be a response to the heating from the ground in the semiarid region from Takla Makan to North China.
Electronic systems for intelligent particle tracking in the high energy physics field

This Ph.D thesis describes the development of a novel readout ASIC for hybrid pixel detector with intelligent particle tracking capabilities in High Energy Physics (HEP) application, called Macro Pixel ASIC (MPA). The concept of intelligent tracking is introduced for the upgrade of the particle tracking system of the Compact Muon Solenoid (CMS) experiment of the Large Hadron Collider (LHC) at CERN: this detector must be capable of selecting at front--end level the interesting particle and of providing them continuously to the back-end. This new functionality is required to cope with the improved performances of the LHC when, in about ten years' time, a major upgrade will lead to the High Luminosity scenario (HL-LHC). The high complexity of the digital logic for particle selection and the very low power requirement of 95% in particle selection and a data reduction from 200 Tb/s/cm2 to 1 Tb/s/cm2. A prototype, called MPA-Light, has been designed, produced and tested. According to the measurements, the prototype respects all the specications. The same device has been used for multi-chip assembly with a pixelated sensor. The assembly characterization with radioactive sources conrms the result obtained on the bare chip
Feature Se lect ion Expert-User Oriented Approach

Abs t rac t . The paper describes the methodology for feature selection and the concept of a user-oriented software package (FS Expert) for feature selection with a consulting system integrated into the package. It attempts to provide a guideline which approach to choose with respect to the extent of a priori knowledge of the problem. The methods implemented in FS Expert are based mostly on the methodology developed by the authors, though it is being built as an open system.
Transverse sectioning in the evaluation of skin biopsy specimens from alopecic dogs

Bearsden, Glasgow, UK 1 Corresponding author email: rbond@rvc.ac. uk O BJECTIVES : Transverse sectioning of skin biopsy specimens has revolutionised assessment of human alopecia by demonstration of every hair in each specimen, allowing quantitative evaluation of follicular activity. Since only vertical sectioning is performed routinely in veterinary laboratories, we aimed to determine whether transverse sectioning was a valuable technique in assessment of canine alopecia. M ETHODS : Paired vertical and transverse sections of biopsy specimens from 31 alopecic dogs were examined independently in triplicate in random order and blinded to previous diagnosis using a standard check-list proforma. Assessments of key features (follicular activity [anagen/telogen], infundibular hyperkeratosis, sebaceous gland abnormalities, pigment clumping, dermal inflammation) by each sectioning method were compared. R ESULTS : In the 31 cases, (atrophic [n = 13], dysplastic [n = 12], inflammatory diseases [n = 6]), follicular inactivity scores (median, [lower-upper quartile]) in transverse sections significantly exceeded those in vertical sections (transverse 4 [3-5], vertical 3 [2-4]). Agreement between
Experimental determination and computational modelling of the damage development during ductile fracture of a free-cutting steel

Ductile fracture of metallic materials is caused by micromechanical damage developing during plastic deformation and finally leading to the initiation and propagation of a crack. Usually the typical damage mechanism is micro-void growth. To characterise this process the micromechanisms of damage have to be taken into regard ("continuum damage mechanics", "local approach"). Local stress and strain properties are calculated by FEM-models to be used for the computational determination of the damage indicators. These damage properties should be able to characterise fracture or crack initiation behaviour independent of stress state (stress triaxiality) or loading history (change of stress triaxiality). Experiments on differently notched tensile specimens have to be done to produce different stress triaxialities. Specimens deformed to a strain lower than the initiation strain are micro-sectioned to measure the damage development in terms of void sizes or void shape parameters. On fracture surfaces of broken specimens the final void size can be measured by SEM observation. Simple ductile damage models are void growth equations. The damage indicator is geometrical. It can be shown that the void size is not constant at failure but depends on the stress state and therefore only gives a rough estimation as a failure criterion. An energy criterium seems to be well fitting to characterise crack initiation in continuum damage mechanics: the damage work density.
Development of a rapid and simpleAgrobacteriumtumefaciens-mediated transformation system for the fungal pathogen Heterobasidionannosum

Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium -mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 1 C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green ﬂuorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green ﬂuorescence protein activity. gentle agitation at 20 1 C. The cocultivation ﬂuid was then spread on Hagems medium plates supplemented with 50 m gmL (cid:2) 1 of hygromycin B and 300 m g mL (cid:2) 1 of cefotaxime. The plates were incubated at 25 1 C in the dark. Heterobasidion annosum colonies were transferred to Hagems medium plates supplemented with hygromycin B (50 m gmL (cid:2) 1 ). After 10 days, the radial growth of the mycelium was determined by the average value from two transects (max & min growth).
Autism Spectrum Disorder and a De Novo Kcnq2 Gene Mutation: A Case Report

The KCNQ2 gene, encoding for the Kv7.2 subunits of the Kv7 voltage potassium channel, is involved in the modulation of neuronal excitability and plays a crucial role in brain morphogenesis and maturation during embryonic life. De novo heterozygous mutations in KCNQ2 genes are associated with early-onset epileptic encephalopathy and neurodevelopmental disorders including developmental delay and intellectual disability. However, little is known about the socio-communicative phenotype of children affected by the KCNQ2 mutation, and a detailed behavioral characterization focused on autistic symptoms has not yet been conducted. This case report describes the clinical behavioral phenotype of a 6-year-old boy carrying a de novo heterozygous KCNQ2 mutation, affected by early-onset seizures and autism spectrum disorder (ASD). We performed a neuropsychiatric assessment of cognitive, adaptive, socio-communicative and autistic symptoms through the administration of standardized tools. The main contribution of this case report is to provide a detailed developmental and behavioral characterization focused on ASD symptoms in a child with [c.812 G > A; p. (Gly271Asp)]mutation in the KCNQ2 gene.
Molecular identiﬁcation of Ca 2 (cid:1) -activated K (cid:1) channels in parotid acinar cells

Molecular identiﬁcation of Ca 2 (cid:1) -activated K (cid:1) channels in parotid acinar cells. Am J Physiol Cell Physiol 284: C535–C546, ajpcell.00044. 2002. —We used molecular biological and patch-clamp techniques to identify the Ca 2 (cid:1) -activated K (cid:1) channel genes in mouse parotid acinar cells. Two types of K (cid:1) channels were activated by intracellular Ca 2 (cid:1) with single-channel conductance values of 22 and 140 pS (in 135 mM external K (cid:1) ), consistent with the intermediate and maxi-K classes of Ca 2 (cid:1) -activated K (cid:1) channels, typiﬁed by the mIK1 ( Kcnn4 ) and mSlo ( Kcnma1 ) genes, respectively. The pres- ence of mIK1 mRNA was established in acinar cells by in situ hybridization. The electrophysiological and pharmacological properties of heterologously expressed mIK1 channels matched those of the native current; thus the native, smaller conductance channel is likely derived from the mIK1 gene. We found that parotid acinar cells express a single, uncom- mon splice variant of the mSlo gene and that heterologously expressed channels of this Slo variant had a single-channel conductance indistinguishable from that of the native, large-conductance channel. However, the sensitivity of this ex- pressed Slo variant to the scorpion toxin iberiotoxin was considerably different from that of the native current. RT- PCR analysis revealed the presence of two mSlo (cid:2) -subunits ( Kcnmb1 and Kcnmb4 ) in parotid tissue. Comparison of the iberiotoxin sensitivity of the native current with that of parotid mSlo expressed with each (cid:2) -subunit in isolation and measurements of the iberiotoxin sensitivity of currents in cells from (cid:2) 1 knockout mice suggest that parotid acinar cells contain approximately equal numbers of homotetrameric channel proteins from the parotid variant of the Slo gene and heteromeric proteins composed of the parotid Slo variant in combination with the (cid:2) 4 -subunit.
HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC STEM BARK EXTRACT OF KNEMA ATTENUATA ( HOO K. F. AND THOMSON) WARB

Objective: To investigate the hepatoprotective activity of ethanolic stem bark extract (ESBE) of Knema attenuata against carbon tetrachloride (CCl 4 ) induced hepatotoxicity in Wistar rats using both in vivo and in vitro models. Methods: Animals were treated orally with ESBE (250 mg kg -1 and 500 mg kg -1 ) once daily for 6 d and CCl 4 on the 4 th d. On the 7 th d, animals were sacrificed and the blood samples were collected to measure the serum levels of biochemical parameters, whereas the liver homogenates were utilized for estimating the antioxidant defense. The hepatoprotective efficacy of the extract was further ensured in vitro using human liver hepatocellular carcinoma (HepG2) cell line against CCl 4 induced toxicity. The cell line viability was determined using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: ESBE effectively reduced (p<0.001) the elevated serum levels of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and Alkaline phosphatase (ALP) when compared to the toxicant control group. ESBE 500 mg kg -1 significantly raised the antioxidant defense (p<0.0001) by reducing the malondialdehyde (MDA) level and enhancing hepatic reduced glutathione (GSH) level in comparison to the CCl 4 control group. The in vitro effect was investigated using CCl 4 exposed HepG2 cells. Pretreatment with ESBE showed a dose-dependent increase in percentage cell viability ranged between 44 to 57% at 12.5-100 μg ml -1 concentrations (p<0.001, when compared to the control cells). Conclusion: Present study confirms the hepatoprotective activity of the stem bark extract of K. attenuata against CCl 4 -induced liver damage. activity [10]. The mechanism of ethanolic extract of stem bark of antioxidant potential. ESBE can reduce free radicals the oxidative damage to the hepatocytes and improve the activities of the liver antioxidant enzymes, thus protecting the liver from CCl induced damage. Its hepatoprotective presence of flavonoids and polyphenolic compounds identified in preliminary phytochemical analysis of extract.