Miracle-dz/UniFAR-FTUs huggingface dataset:
Prevalence and clonality of methicillin-resistant (MRSA) in the Atlantic Azores islands: predominance of SCC types IV, V and VI

In order to obtain insights into the methicillinresistant Staphylococcus aureus (MRSA) population structure in the Azores archipelago, 106 MRSA isolates were collected from patients attending an Azorean central hospital between January 2007 and February 2008. Antimicrobial resistance was determined for all isolates. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE), spa typing, multilocus sequence typing (MLST), staphylococcal chromosome cassette mec (SCCmec) typing and the presence of Panton–Valentine leukocidin (PVL). The majority of the isolates (87%, n=92) belonged to the EMRSA-15 clone (ST22, SCCmec-IVh), followed by the Pediatric clone (ST5-VI/IVc) (11%, n = 12). The Berlin clone (ST45-IVa) and a new clone (spa type t1839, ST1339 and SCCmec V variant) were represented by single isolates. All of the isolates carried SCCmec types IV, V or VI and a non-multiresistant antibiotic profile, resembling the currently emerging community MRSA. Moreover, PVL was described for the first time to be associated with the Pediatric clone carrying SCCmec type VI. We provided the first description of the population structure of MRSA in the Azores islands, which seems to be shaped by genetic events occurring locally, as well as by the regular population exchange between the islands, continental Portugal, the United Kingdom and the United States.
Maximum Power Point Tracking of Dish Stirling System in Partially Shadow condition using Fuzzy Logic Controller based on PSO Algorithm

This paper presents a novel approach based on fuzzy logic controller to track optimum operational point of dishstirling system in uniform irradiance condition. The proposed method considers all details of system including thermo mechanical and electrical aspects of dish stirling system where previous studies use a simple model to assess this issue. Multi parallel dish stirling systems have more extremum operation points in partially condition system. Hence, this study utilize fuzzy logic controller to find global maximum power point tracking of system when all aspects of system are taken in to account. Finally, In order to validate the proposed method, a comprehensive case study has been conducted on dish stirling systems. Simulation results show the accuracy of the proposed method in different conditions in comparison with previous methods.
Outcomes In Newborn Preterm Lambs

*joint first authors 8 9 1 Neonatal Research, Murdoch Childrens Research Institute, Parkville, Australia. 10 2 Neonatology, The Royal Children’s Hospital, Parkville, Australia 11 3 Neonatal Research, The Royal Women’s Hospital, Parkville, Australia 12 4 Department of Paediatrics, University of Melbourne, Melbourne, Australia 13 5 NICU, Fondazione IRCCS Ca’ Granda, Ospedale Maggiore Policlinico-Università 14 degli Studi di Milano, Milano, Italy. 15 6 TBM Lab, Dipartimento di Elettronica, Informazione e Ingegneria Biomedica-DEIB, 16 Politecnico di Milano University, Milano, Italy. 17 7 Systems and Computer Engineering, Carleton University, Ottawa, Canada 18 8 Fraunhofer Project Group for Automation in Medicine and Biotechnology, 19 Mannheim, Germany 20 9 Department of Anaesthesiology and Intensive Care Medicine, University Medical 21 Centre Schleswig-Holstein, Campus Kiel, Kiel, Germany 22 10 Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, 23 Australia. 24 25 Corresponding Author: 26 A/Prof David G Tingay MB BS DCH FRACP Ph.D. 27 Neonatal Research 28 Murdoch Childrens Research Institute 29 Royal Children’s Hospital 30 Flemington Rd. 31 Parkville 3052 Victoria 32 Australia 33 +61 3 9345 4023 34 +61 3 9345 5067 (fax) 35 david.tingay@rch.org. au 36 37 Running head: Fixed Duration vs Volume-Response Defined SI at birth. 38 39 Word Count: 5447 words 40 41 Articles in PresS. Am J Physiol Lung Cell Mol Physiol (September 25, 2015). doi:10.1152/ajplung.00277. 2015
How Male versus Female Shoppers’ Feeling, Thinking, and Personality Impact Satisfaction

Shopper satisfaction is among the top interest of marketers. This is because satisfaction leads to sales and profit, both now and in the future. This paper approaches satisfaction from the shopper point of view. In particular, we propose a conceptual framework to depict how the feeling, thinking, as well as personality of the shoppers would impact their satisfaction. The authors also compare gender (male versus female) to see how those factors differently impact on satisfaction of shoppers who have different gender. To test the research hypotheses and to compare the impact of the factors determining the differences in shopper satisfaction between male and female, the authors use multiple regression with multi-group techniques. The results show that factors influencing satisfaction of shoppers are different across genders. The findings of this paper would help scholars and practitioners to better understand shoppers, and that practitioners could apply the findings in their marketing practice in order to better satisfy their shoppers.
Development of Yawning Detection Algorithm for Normal Lighting Condition and IR Condition

— Drowsiness monitoring system has been widely used in this current technology to monitor the driver’s state while driving. This paper presents a drowsiness detection method through the activity of yawning for both normal lighting condition and Infrared (IR) condition. Development of the algorithm consists of several steps. Initially, the detection of face and mouth implementing the Viola-Jones algorithm takes place. For IR condition, the mouth is detected by applying the geometrical measurements of a face. After the detection process is done, the tracking process for both face and mouth takes place utilizing the Kanade-Lucas-Tomasi (KLT) algorithm which is basically a point tracking algorithm. Based on the tracked mouth, the region of interest (ROI) is selected which is to be used as an input image in the image processing step in order to get a clearer image of the mouth. From the finalized mouth image in the preprocessing step, the properties of the image are further used in the yawning detection step. In the indication of yawning, the height of the mouth opening reading score is observed. The performance of the proposed method is tested on 5 subjects and achieved an overall accuracy of 98.89% for normal lighting condition and 95.29% for IR condition.
Quantitative Detection of Burkholderia pseudomallei in Soil samples Using Multiple Gene Targets in Real Time PCR

6 1 Friedrich Loeffler Institute of Medical Microbiology, University Medicine Greifswald, 7 Greifswald, Germany; 8 2 Institute of Hygiene, Microbiology and Environmental Medicine, Medical University 9 Graz, Graz, Austria 10 Institute of Microbiology and Biotechnology, Vietnam National University, Hanoi, 11 Vietnam 12 Institute for Geography and Geology, Ernst-Moritz-Arndt-University Greifswald, 13 Greifswald, Germany; 14 Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, 15 Mahidol University, Bangkok, Thailand; 16 London School of Hygiene and Tropical Medicine, London, United Kingdom; 17 Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, 18 Bangkok, Thailand; 19 Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA 20
Ribosome hibernation facilitates tolerance of stationary-phase bacteria to 1 aminoglycosides 2 3

1 aminoglycosides 2 3 Susannah L. McKay and Daniel A. Portnoy 4 5 Department of Molecular and Cellular Biology, and School of Public Health, 6 University of California, Berkeley, California 94720-3202 7 8 Running title: HPF mediates tolerance to aminoglycosides 9 10 # Corresponding author. Mailing addresses: Department of Molecular & Cell 11 Biology, 510 Barker Hall MC-3202, University of California, Berkeley, Berkeley, 12 CA 94720-3202. Phone (510) 643-3926. Fax (510) 643-6334. E-mail: 13 portnoy@berkeley.edu. 14 15
Pre-proof First report of a bla VIM-1 metallo-β-lactamase-possessing Klebsiella michiganensis

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Proliferation to Evaluate Preliminarily the Presence of Enduring Self ‐ Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

(1) Background: a cell evaluation focused to verify the self‐regenerative antioxidant activity is performed on cerium doped bioactive glasses. (2) Methods: the glasses based on 45S5 Bioglass®, are doped with 1.2 mol%, 3.6 mol% and 5.3 mol% of CeO2 and possess a polyhedral shape (~500 μm2). Glasses with this composition inhibit oxidative stress by mimicking catalase enzyme (CAT) and superoxide dismutase (SOD) activities; moreover, our previous cytocompatibility tests (neutral red (NR), 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) and Bromo‐2‐deoxyUridine (BrdU)) reveal that the presence of cerium promotes the absorption and vitality of the cells. The same cytocompatibility tests were performed and repeated, in two different periods (named first and second use), separated from each other by four months. (3) Results: in the first and second use, NR tests indicate that the presence of cerium promotes once again cell uptake and viability, especially after 72 h. A decrease in cell proliferation it is observed after MTT and BrdU tests only in the second use. These findings are supported by statistically significant results (4) Conclusions: these glasses show enhanced proliferation, both in the short and in the long term, and for the first time such large dimensions are studied for this kind of study. A future prospective is the implantation of these bioactive glasses as bone substitute in animal models.
Numerical Modeling the Flood and Pollutant Transport Processes in Residential Areas with Different Land Use Types

Large-scale flooding causes widespread disaster, and harmful pollutant concentration in water following flood affects public safety and the environment. In this study, a numerical model for solving the 2D shallow water equations and the solute transport equation is proposed to simulate overland flood and pollutant transport caused by floods. ,e present model is verified by comparing the predictions with the analytical solutions and simulation results; sufficiently high computational accuracy is achieved. ,e model is also used to simulate flood inundation and pollution spread in the area of Hun and Taizi Lane (HTL) in China due to river dike breaches; the results show that the coupling model has excellent performance for simulating the flooding process and the temporal and spatial distribution of pollutants in urban or rural areas. We use remote sensing techniques to acquire the land coverage in the area of HTL based on Landsat TM satellites. ,e impacts of changed land use on mitigation of flooding waves and pollutant spread are investigated; the results indicate that the land cover changes have an obvious influence on the evolution process of flood waves and pollutant transport in the study areas, where the transport of pollutants is very dynamic during flood inundation in HTL area. Furthermore, the motion of pollutants considering anisotropic diffusion is more reasonable than that due to isotropic dispersion in simulating pollutant transport associated with the flood in urban or farmland environments.
Essential Cell Division Protein in Escherichia coli K-12

ftsQ is an essential cell division gene in Escherichia coli. The ftsQ gene has been sequenced, and a presumptive open reading frame has been identified; however, no protein product has been observed (A. C. Robinson, D. J. Kenan, G. F. Hatfull, N. F. Sullivan, R. Spiegelberg, and W. D. Donachie, J. Bacteriol. 160:546-555, 1984, and Q.-M. Yi, S. Rockenbach, J. E. Ward, Jr., and J. Lutkenhaus, J. Mol. Biol. 184:399-412, 1985). The ftsQ gene was isolated on a 970-base-pair EcoRI-PvuII fragment of the E. coli chromosome and used to construct a trp-lac (PtaC) transcriptional fusion in plasmid pKK223-3. The fused construct (pDSC78) complemented anftsQI(Ts) mutant strain in trans, restoring growth at 42°C on low-salt medium. AnftsQI(Ts) mutant strain transformed with pDSC78 appeared normal upon microscopic examination, with no indication of filamentation. TheftsQ gene product was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis of radiolabeled, isopropyl-|1-D-thiogalactopyranoside-induced maxicell and normal cell extracts. As predicted from the nucleotide sequence, the 970-base-pair EcoRI-PvuII fragment encoded a polypeptide of approximately 31,400 daltons. Analysis of the data obtained from pulse-chase experiments in maxicells and normal cells suggests that the FtsQ protein is stable. Most of the radiolabeled FtsQ protein from maxicells was found in the inner membrane. On the basis of available information, the prior inability to detect FtsQ can be attributed to low levels of transcription or translation rather than to proteolysis.
Protein Antigens in Adjuvants Presentation during the Immune Response to Neutrophils Influence the Level of Antigen

Neutrophils modulated Ag presentation following immunization with Ags in CFA or IFA or alum. The neutrophils had an important negative role in the CD4 T cell and B cell responses to three protein Ags: hen egg white lysozyme, OVA, and listeriolysin O. In their absence (by depleting with Abs for only the ﬁrst 24 h, or using genetically neutropenic mice), the cellular responses increased several-fold. The CD8 response was not affected or slightly decreased. Competition for Ag between the presenting cells and the neutrophils, as well as an effect on the response to Ag-bearing dendritic cells (DCs), was documented. Neutrophils entered the draining lymph nodes rapidly and for a brief period of several hours, localizing mainly to the marginal sinus and superﬁcial cortex. There they established brief contact with DCs and macrophages. Moreover, neutrophils imprinted on the quality of the subsequent DC–T cell interactions, despite no physical contact with them; by intravital microscopy, the clustering of Ag-speciﬁc T cells and DCs was improved in neutropenic mice. Thus, neutrophils are obligate cells that brieﬂy enter sites of immunization and set the level of Ag presentation. A brief depletion may have a considerably positive impact on vaccination. The Journal of Immunology , 2010, 185: 000–000.
Health benefits of reducing NOx emissions in the presence of epidemiological and atmospheric nonlinearities

Recent epidemiological evidence suggests that the logarithmof concentration is a better predictor of mortality risk from long-termexposure to ambientPM2.5 andNO2 than concentration itself. A logconcentration-response function (CRF)predicts a heightened excess risk per unit concentration at low levels of exposure that further increases as the air becomes less polluted.Using an adjoint air quality model, we estimate the public health benefits of reducingNOx emissions, on a per-ton and source-bysource basis.Our estimates of benefits-per-ton assume linear in concentration and log-concentration CRFs forNO2 and aCRF that is linear in concentration forO3.We apply risk coefficients estimated using theCanadianCensusHealth andEnvironmentCohort.Wefind that a log-concentrationCRF for NO2 leads almost consistently to larger benefits-per-ton than a linear in concentrationCRF (e.g., $500 000 ton compared to $270 000 ton forOttawa). We observe that concentrations gradually decline due towidespread, progressive emissions abatement, entailing increasinghealth benefits as a result of (1) a log-concentrationCRF forNO2 and (2) the nonlinear response ofO3 toNOx emissions. Our results indicate thatNOx abatement has the potential to incur substantial and increasing health benefits, by up tofive timeswith 85%emission reductions, forCanada into the future.
Cleistogamous spike and chasmogamous spike carbon remobilization improve the seed potential yield of Cleistogenes songorica under water stress

Abstract Developmental signals and environmental stresses regulate carbon distribution in the vegetative and reproductive organs of plants and affect seed yield. Cleistogenes songorica is a xerophytic grass with great potential application value in ecological restoration. However, how carbohydrate transport and distribution during grain filling affect the seed yield of C. songorica under water stress is not clear. The present study showed that the soluble sugar and starch contents of cleistogamous (CL) spikes and chasmogamous (CH) spikes were significantly higher at the milk stage, which was attributed to a significantly higher seed number and seed yield per spike under water stress conditions than under well-watered conditions (P < 0.01). RNA-seq data revealed a total of 54,525 differentially expressed genes (DEGs) under water stress conditions, but only 3744 DEGs were shared among all comparison groups. Weighted gene co-expression network analysis showed that the transport and distribution of carbohydrates were regulated by ABA-responsive genes (CsABA8OX1_1, CsABA8OX1_2, CsABA8OX2_1, CsABA8OX2_2, CsNCED3, CsNCED1_1, CsNCED1_2 and CsNCED4_1) and sugar transport and starch synthesis genes (CsSUS1, CsSUS2, CsSUS3, CsAGP1, CsAGP4, CsAGP5, CsSSS1 and CsSBE5) under water stress conditions. These genes jointly regulated carbohydrate remobilization in sources (stems, leaves and sheaths) to promote grain filling and improve seed yield. The present study helped to clarify the phenotypic, metabolic and transcriptional response mechanisms of vegetative organs, such as stems and leaves, and reproductive organs, such as CL spikes and CH spikes, to promote carbohydrate redistribution under water stress, and it provides theoretical guidance for improving seed yields.
University of Birmingham Human rights violations, political conditionality and public attitudes to foreign aid

There has been much criticism of donor governments who give aid to states that violate human rights. This has fuelled concerns about how such coverage affects public support for foreign aid. In response, donors increasingly use aid suspensions to signal to domestic audiences that a regime has been sanctioned and aid is not misspent. This article examines how reports of rights violations affect attitudes to aid and what, if any, impact donor responses have on public perceptions. We conduct survey experiments using nationally representative samples of the British public. Our findings demonstrate that reports of rights abuses reduce public support for aid. However, contrary to conventional wisdom, any response from donors, whether it be to justify continuing aid or to cut aid, prevents a decline in support. In policy terms, the findings demonstrate the importance of government responsiveness in maintaining public support for a frequently contested aspect of foreign policy.
DDDT_A_260419 3589..3599

1Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; 2Pharmaceutics Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; 3Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran; 4Neuroscience Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran; 5Afzal Research Institute (NGO), Kerman University of Medical Sciences, Kerman, Iran Purpose: Human butyrylcholinesterase (BChE) serves as a bio scavenger to counteract organophosphate poisoning. It is also a potential drug candidate in several therapeutic fields. Therefore, in the present study, we constructed a new dual-promoter plasmid consisting of Cytomegalovirus (CMV) and human elongation factor 1α (EF-1α) promoters and transfected that into HEK-293 cells using Lipofectamine to enhance the BChE secretion. Methods: The new dual-promoter construction (pBudCE dual BChE) including two copies of the BChE gene was designed and transfected into cells by liposomal structures. The cloned plasmids were evaluated by enzyme digestion and gel electrophoresis analysis. Experimental groups were categorized into the cells transfected by pBudCE dual BChE (treatment), pCMV (positive control) vectors, and nontransfected cells (negative control). BChE gene expression was evaluated by qRT-PCR and the enzyme activity was assessed using modified Ellman’s method. The freeze-thaw process was carried out for analyzing the stability of the pBudCE dual BChE vector. Results: Validation examination of the cloned plasmids confirmed the successful cloning process. The gene expression level and Ellman’s method value in pBudCE dual BChE was higher than the other groups. CMV promoter has also increased the enzyme activity, although the difference was not significant compared with the control group. Interestingly, freeze-thaw cycles followed by several passages did not affect the enzyme activity. Conclusion: The designed construction with CMV and EF-1α promoters could increase BChE gene expression and the activity of the BChE enzyme in HEK-293 cell line. Largescale production of BChE enzyme can be achieved by using dual-promoter plasmid construction compared to a single-promoter vector to be used in clinical trials.
The DNA Damage response and cell competition are p53- and Xrp1-dependent processes that suppress hyperplastic aneuploidy

It is important to understand how p53 suppresses tumorigenesis. P53 activity contributes to many instances of cell competition in mammals. This has not been seen for Drosophila p53, where the transcription factor Xrp1 is an effector of cell competition. Xrp1 is induced in a p53-dependent manner by DNA damage, and we report that Xrp1 mediates multiple functions of p53 in the DNA damage response, contributing to p53-dependent gene transcription and DNA damage-induced apoptosis. Differences in either Xrp1 or p53 activity, occurring between wild type and mutant cells experiencing mild genotoxic stress, both resulted in cell competition. Unexpectedly, cell competition due to differential p53 activity did not require Xrp1 but instead was restrained by Xrp1. We show that Xrp1 has a p53-independent role in removing genomically-altered cells. Both Xrp1 and p53 limit the accumulation of abnormal cells that results from genotoxicity, and we propose that genomic alterations enhance cellular growth of p53 mutant cells and promote cell competition, potentially contributing to the tumorigenesis in p53 mutants.