Miracle-dz/UniFAR-FTUs huggingface dataset:
Tear Meniscus Height Determination Using the OCT2 and the RTVue-100

Purpose. To compare anterior segment spectral-domain optical coherence tomography (SOCT) tear meniscus height (TMH) measures with those from the more commonly used time-domain OCT (TOCT). Methods. The right eye of 50 healthy subjects had images of their TMH captured with TOCT (OCT2) and SOCT (RTVue-100). Data were acquired using two different anterior segment lenses, the cornea/anterior module-short (CAM-S) and long (CAM-L), on the SOCT. Images were then analyzed for differences in their derived TMH. Results. The average TMH for TOCT was 0.280 ± 0.139 mm, whereas the mean TMH measured using the SOCT was 0.354 ± 0.163 mm and 0.345 ± 0.167 mm for the CAM-S and CAM-L, respectively. There was a significant difference (p < 0.001) when comparing TOCT with either of the SOCT lenses. There was no statistically significant difference between the CAM-L and CAM-S (p = 1.0). Bland-Altman analysis showed poor agreement between TOCT and SOCT (95% limits of agreement −0.138 to + 0.285 mm for the CAM-S and −0.185 to +0.315 mm for the CAM-L). Conclusions. The RTVue-100 produces TMH measurements that are significantly higher than OCT2 in a normal patient population. However, the RTVue-100 showed a number of other advantages over the OCT2 in the measurement and analysis of images. Future work needs to determine the causative factors behind the observed differences.
Application of Immunoreaction Enhancer Solutions to an Enzyme-Linked Immunosorbent Assay for Antigen-Specific IgE in Mice Immunized with Recombinant Major Mite Allergens or Ovalbumin

Background: Weak signals for allergen-specific IgE are a problem in murine models for the study of allergies. It has been reported that the removal of IgG from murine sera enhances signal intensity. Very recently, buffer solutions designed to enhance signals in immunoassays have been developed and made commercially available. Methods: Sera from mice immunized either with a recombinant form of one of the major mite allergens Der p 1, Der f 1 and Der f 2, or with ovalbumin adsorbed to alum were used for the assays. Total IgE was measured by a sandwich enzyme-linked immunosorbent assay (ELISA). Allergen-specific IgE was assayed using plates coated with the allergens after the removal of IgG from sera with protein G-coupled sepharose beads in wells of other plates or with the use of commercially available enhancer solutions without the removal of IgG. IgE binding was detected with horseradish peroxidase-conjugated anti-murine IgE monoclonal antibody as the secondary antibody. Results: Significant levels of total IgE were produced after the immunizations. The in-well pretreatment of diluted sera (1/10 dilution) with protein G-coupled beads enhanced the signals for allergen-specific IgE. The use of the enhancer solutions for dilution of the sera and secondary antibody and prolonged incubation remarkably enhanced the signals at a more extensive dilution of sera (1/200 or less) without the removal of IgG. Conclusions: An ELISA simply modified with the use of immunoreaction enhancer solutions has advantages in terms of signal intensity and ease of handling for the detection of allergen-specific murine IgE and would be useful for the study of allergies with murine models.
CHD5 a tumour suppressor is epigenetically silenced in hepatocellular carcinoma

Chromodomain helicase DNA binding protein 5 (CHD5) has recently been identified as a potent tumour suppressor by acting as a master regulator of a tumour‐suppressive network. Its inactivation resulted from aberrant methylation in the promoter occurs in several types of human malignancy and is associated with malignant tumour behaviour. In human hepatocellular carcinoma (HCC), CHD5 gene expression, methylation status and tumour‐suppressive function have not been elucidated.
Development and preliminary validation of the cognitive behavioral dieting scale.

OBJECTIVE The purpose of this research was to empirically develop the Cognitive Behavioral Dieting Scale (CBDS), a measure of current dieting. METHOD The first study involved item generation and a procedure to boost internal consistency while reducing scale length. Study 2 involved a factor analysis and measures of scale reliability. The third study evaluated the ability of the CBDS to predict calorie intake and negative calorie balance from a 24-hr diet recall. Study 4 evaluated construct validity by comparing the CBDS to dietary restraint, body image, and health behavior self-efficacy. RESULTS The CBDS is a 14-item scale which measures current dieting behavior and related thoughts within the past 2 weeks. Internal consistency was alpha = . 95 and 2-day test-retest reliability was r = . 95. This scale provides a method for operationalizing dieting, provides a construct that is different from restraint, and assess dieting behavior on a continuum. Additionally, this scale was able to predict calorie intake and negative calorie balance above and beyond the predictive ability of physical variables (i.e., body mass index BMI] and exercise calories). An additional study of construct validity showed the CBDS was related to poor body image esteem and dietary restraint, but minimally related to healthy eating self-efficacy. DISCUSSION In conclusion, the CBDS shows promise as a valid and reliable measure of dieting behavior. This scale should have utility in future research on how current dieting relates to eating disorders, dietary restraint, and obesity.
Identification of the bona fide DHDPS from a common plant pathogen

Agrobacterium tumefaciens is a Gram‐negative soil‐borne bacterium that causes Crown Gall disease in many economically important crops. The absence of a suitable chemical treatment means there is a need to discover new anti‐Crown Gall agents and also characterize bona fide drug targets. One such target is dihydrodipicolinate synthase (DHDPS), a homo‐tetrameric enzyme that catalyzes the committed step in the metabolic pathway yielding meso‐diaminopimelate and lysine. Interestingly, there are 10 putative DHDPS genes annotated in the A. tumefaciens genome, including three whose structures have recently been determined (PDB IDs: 3B4U, 2HMC, and 2R8W). However, we show using quantitative enzyme kinetic assays that nine of the 10 dapA gene products, including 3B4U, 2HMC, and 2R8W, lack DHDPS function in vitro. A sequence alignment showed that the product of the dapA7 gene contains all of the conserved residues known to be important for DHDPS catalysis and allostery. This gene was cloned and the recombinant product expressed and purified. Our studies show that the purified enzyme (i) possesses DHDPS enzyme activity, (ii) is allosterically inhibited by lysine, and (iii) adopts the canonical homo‐tetrameric structure in both solution and the crystal state. This study describes for the first time the structure, function and allostery of the bona fide DHDPS from A. tumefaciens, which offers insight into the rational design of pesticide agents for combating Crown Gall disease. Proteins 2014; 82:1869–1883. © 2014 Wiley Periodicals, Inc.
Current considerations in pediatric speech audiometry

Current considerations in pediatric speech perception assessment are highlighted in this article with a focus on specific test principles and variables that must be addressed when evaluating speech perception performance in children. Existing test materials are reviewed with an emphasis on the level of sensitivity and standardization that they have for accurate assessment of a child's speech perception performance. A test battery approach is advocated because speech perception is an abstract construct, and in order to provide a comprehensive assessment of a child's capabilities, information is needed from several sources of concrete data. The importance of ongoing speech perception assessment in children is also emphasized because a child's progress over time must be monitored to determine if improvements need to be made with amplification and intervention efforts. Results from pediatric speech perception assessments can provide practical information regarding the prognosis of speech, language, reading, and cognitive abilities of children as well as steps that need to be taken in the intervention process.
Integration of physiological and accelerometer data to improve physical activity assessment.

PURPOSE Accurate measurement of physical activity (PA) is a prerequisite to determine dose-response relationships between activity and health. The combination of HR and accelerometers (ACC) holds promise for improving the accuracy of PA assessment, but it is unclear how currently proposed modeling techniques compare and to what extent different levels of individual calibration (IC) of HR influence monitoring accuracy. METHODS A total of 10 men and women (25.8 +/- 3.4 yr, 1.70 +/- 0.1 m, 71.7 +/- 11.8 kg, 24.4 +/- 5.0 kg.m-2) were recruited for this study, in which IC of HR to PA energy expenditure (PAEE) during both arm crank and treadmill activity were available. Participants completed 6 h of free-living activity, during which PAEE (obtained with indirect calorimetry), HR, hip ACC, arm ACC, and leg ACC were collected. PAEE was then modeled from two different methods of combining HR and ACC (arm-leg HR+M and branched model), both with IC and group-level calibration (GC) of HR, and also from hip ACC estimates alone. Estimates of PAEE were compared with criterion values for PAEE. RESULTS Combined estimates of PAEE from the arm-leg HR+M and the branched model were similar when IC was used (R2 = 0.81, SEE = 0.55 METs and R2 = 0.75, SEE = 0.61 METs, respectively). When using GC, all estimates of PAEE had larger error, but the performance of the branched model suffered less than the arm-leg HR+M model (R2 = 0.75, SEE = 0.67 METs and R2 = 0.67, SEE = 0.88 METs, respectively). Both combination modeling techniques were more precise than single-measure hip ACC estimates (R2 = 0.41, SEE = 0.96 METs). CONCLUSION The combination of HR and ACC improves the accuracy of PAEE estimates and could be applied in large-scale epidemiological studies.
Glycosylation Defect in Lec1 Chinese Hamster Ovary Mutant Is Due to a Point Mutation in N-Acetylglucosaminyltransferase I Gene*

The Lec1 Chinese hamster ovary (CHO) mutant is a leuco-phytohemagglutinin resistant cell line unable to synthesize complex and hybrid N-glycans due to the lack of N-acetylglucosaminyltransferase I (GnTI) activity. Here we have identified the lec1 mutation. Using specific antibodies to GnTI we demonstrate that Lec1 cells synthesize an inactive GnTI protein identical in size to the wild-type CHO enzyme. We have cloned and sequenced the gene coding GnTI from parental CHO and Lec1 mutant cells. Comparison of GnTI sequences detected three mutations within the luminal domain of Lec1 GnTI, each resulting in an amino acid substitution. The effect of each mutation on enzyme activity was analyzed by site-directed mutagenesis of wild-type rabbit GnTI and transient expression in COS cells. One of the three mutations (Cys123 → Arg123) resulted in complete loss of activity, whereas the other two mutations had no apparent effect on enzyme activity. This conclusion was confirmed by expression of GnTI mutants in the GnTI null background of Saccharomyces cerevisiae. Both Lec1 GnTI and the GnTI mutant (Cys123 → Arg123) are correctly localized to the Golgi apparatus, indicating that the inactive GnTI molecules are sufficiently well folded for efficient transport from the endoplasmic reticulum. These results demonstrate that the lec1 mutation is a point mutation and that Cys123 is a critical residue for GnTI activity.
Electromagnetic analysis of a PMSM with different rotor topologies

This paper realizes a study of the influence of specific rotor design parameters on the permanent magnet synchronous machine (PMSM) electromagnetic behavior. The study is carried out in a FEM analysis environment. Four permanent magnet synchronous machines with the same stator and different rotor topologies have been simulated and their electromagnetic characteristics are compared to each other. The rotor topologies with surface-mounted magnets, magnets embedded in the surface, tangentially embedded magnets and cross buried magnets are considered. The simulations performed offer an insight and comparison between the four motors electromagnetic variables and behavior under no load and full load with two different stator currents and the same speed operation.
A holonic approach for task scheduling in manufacturing systems

This paper deals with a new architecture and negotiation protocol for the dynamic scheduling of manufacturing systems. The architecture is based on two paradigms: multi-agent systems and holonic systems. The main contribution in the architecture is the existence of holons representing tasks together with holons representing resources. The well known contract net protocol has been adapted to handle temporal constraints and to deal with conflicts. The purpose of this protocol is to dynamically assign operations to the resources of the manufacturing system in order to accomplish the proposed tasks. This protocol involves a renegotiation phase whenever exceptions appear. It also deals with conflict situations, namely with the case of the "indecision problem". The approach we are using assumes that deadlines are the most important constraints to consider, thus the acceptance or refusal of a resource for a specific operation depends on the capability of executing the operation in the specified deadline.
A Case of VoIP Implementation at A Financial Services Office

Customer service and customer retention are critical to an organization’s success in the service sector. In the Midwest retail office of Anthem Consultants, the old PBX system was incapable of handling customer inquires during the busy tax return season. The inefficient systems exposed the organization to missed and delayed calls, which lead to a considerable number of customer complaints and lost revenue. This paper presents a case for implementing a VoIP PBX system at Anthem Consultants. Several VoIP systems were tested to determine the suitability of VoIP as the replacement telecommunications platform. The new system increases the availability, speed, and reliability of the information provided to customers and at the same time reduces the staff load. The system implementation also fosters an updated IT plan that will help this organization chart its business strategy for future years.
Purification and characterization of Staphylococcus aureus FRI 1169 and 587 toxic shock syndrome exotoxins

An exotoxin was purified from a toxic shock toxin (TST)-producing Staphylococcus aureus strain, FRI 1169, and another exotoxin was purified from a pyrogenic exotoxin C (PEC)-producing S. aureus strain, 587. Both strains had been isolated from toxic-shock syndrome patients. The two exotoxins were purified by the same method of ion-exchange chromatography, chromatofocusing, and gel filtration. After purification, those exotoxins gave a line of identity against an anti-TST serum and also were immunologically similar to TST in a double-diffusion test. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, each exotoxin gave a single band with a relative mobility identical to that of the other. Their molecular weights (24,000), isoelectric points (7.0), amino acid compositions, and NH2-terminal amino acid sequences (the first four residues) were identical. Both produced fever and enhanced host susceptibility to lethal endotoxin shock in rabbits, comparable with PEC. These findings show that the two exotoxins are the same protein, which is assumed to be TST. When injected into rabbits, the culture supernatant of strain 587 showed biological activity like that described above, whereas the culture supernatant neutralized with anti-TST immunoglobulin did not. This showed that PEC-producing strain 587 does not produce any toxin with these biological activities in rabbits except TST.
On the microphysical effects of observed cloud edge charging

Liquid layer clouds are abundant globally. Lacking strong convection, they do not become electrified by the usual thunderstorm mechanisms of collisional electrification between hydrometeors of different phases. Instead, the background global circuit current flow in fair weather is largely unaffected by the layer cloud's presence, and, if the layer cloud is extensive horizontally, the vertical fair weather conduction current passes through the cloud. A consequence of the vertical current flow is that, at the cloud–air boundary where there is a conductivity transition and droplets form or evaporate, droplet charging occurs. Charge can affect both droplet evaporation and droplet–droplet collisions. Using new radiosonde instrumentation, the charge observed at layer cloud edges is evaluated for both these microphysical droplet processes. This shows that the charging is more likely to affect collision processes than activation, for small droplets. Enhancing the collection efficiency of small droplets modifies their evolution and propagates through the size distribution to shorten the autoconversion time‐scale to rain drops, and the cloud radiative properties. Because the conduction current density is influenced by both external (e.g. solar modulation of high‐energy particles) and internal (e.g. El Niño–Southern Oscillation) factors, current flow leading to layer cloud edge charging provides a possible route for expressing solar influences on the climate system and a teleconnection mechanism for communicating internal climate variability.
Predicting dynamic computational workload of a self-driving car

This study aims at developing a method that predicts the CPU usage patterns of software tasks running on a self-driving car. To ensure safety of such dynamic systems, the worst-case-based CPU utilization analysis has been used; however, the nature of dynamically changing driving contexts requires more flexible approach for an efficient computing resource management. To better understand the dynamic CPU usage patterns, this paper presents an effort of designing a feature vector to represent the information of driving environments and of predicting, using regression methods, the selected tasks' CPU usage patterns given specific driving contexts. Experiments with real-world vehicle data show a promising result and validate the usefulness of the proposed method.
Unique Glycan Signatures Regulate Adeno-Associated Virus Tropism in the Developing Brain

ABSTRACT Adeno-associated viruses (AAV) are thought to spread through the central nervous system (CNS) by exploiting cerebrospinal fluid (CSF) flux and hijacking axonal transport pathways. The role of host receptors that mediate these processes is not well understood. In the current study, we utilized AAV serotype 4 (AAV4) as a model to evaluate whether ubiquitously expressed 2,3-linked sialic acid and the developmentally regulated marker 2,8-linked polysialic acid (PSA) regulate viral transport and tropism in the neonatal brain. Modulation of the levels of SA and PSA in cell culture studies using specific neuraminidases revealed possibly opposing roles of the two glycans in AAV4 transduction. Interestingly, upon intracranial injection into lateral ventricles of the neonatal mouse brain, a low-affinity AAV4 mutant (AAV4.18) displayed a striking shift in cellular tropism from 2,3-linked SA+ ependymal lining to 2,8-linked PSA+ migrating progenitors in the rostral migratory stream and olfactory bulb. In addition, this gain-of-function phenotype correlated with robust CNS spread of AAV4.18 through paravascular transport pathways. Consistent with these observations, altering glycan dynamics within the brain by coadministering SA- and PSA-specific neuraminidases resulted in striking changes to the cellular tropisms and transduction efficiencies of both parental and mutant vectors. We postulate that glycan signatures associated with host development can be exploited to redirect novel AAV vectors to specific cell types in the brain. IMPORTANCE Viruses invade the CNS through various mechanisms. In the current study, we utilized AAV as a model to study the dynamics of virus-carbohydrate interactions in the developing brain and their impact on viral tropism. Our findings suggest that carbohydrate content can be exploited to regulate viral transport and tropism in the brain.
Partial Breast Brachytherapy as the Primary Treatment for Breast Cancer Diagnosed After Mantle Radiation Therapy for Hodgkin's Disease

Purpose:Mastectomy is the most widely accepted treatment for managing breast cancer in patients who have previously received mantle radiation therapy (RT). With accumulating evidence supporting partial breast irradiation, we treated newly diagnosed breast cancer among lymphoma survivors who had history of RT with lumpectomy and breast brachytherapy. Methods:Five patients, with history of Hodgkin disease (HD) underwent lumpectomy and sentinel lymphadenectomy ± axillary dissection followed by low dose rate Ir-192 interstitial brachytherapy. A multicatheter technique was used and all patients received 45 Gy to the target volume over 4 to 5 day time interval. Adjuvant systemic therapy when indicated was administered after completion of brachytherapy. Results:The median time from the treatment of the HD to the diagnosis of breast cancer was 242 months (range: 68–322 months). The median T-size of the invasive breast cancer is 0.7 cm. The range of follow up after brachytherapy is 5 to 67 months. All 5 patients have an intact breast and are without evidence of relapse. No patients developed an infection. No skin toxicity ≥grade 2 was observed. All patients have an excellent cosmetic result. No excessive fibrosis or necrosis has been observed. Conclusion:The preliminary experience using partial breast brachytherapy (PBB) in patients with previous history of mantle RT suggests low complication rates, and acceptable cosmetic results. The excellent local control and freedom from mastectomy for local recurrence warrants further investigation to establish brachytherapy as an acceptable alternative to mastectomy for early stage breast cancer among HD survivors with history of prior RT.