Miracle-dz/UniFAR-FTUs huggingface dataset:
Capillary malformation as a locus minoris resistentiae for the development of basal cell carcinoma

znidazole (5 mg/kg/day) for 30 days with total remission of lesions. A 62-year-old male presented with a 10-day history of a painful erythematous-brownish plaque on the left foot (Fig. 1c). Five months previously, he had received a heart transplant due to Chagas cardiomyopathy and was taking tacrolimus (6 mg), mycophenolate mofetil (360 mg) and prednisone (15 mg). A biopsy specimen showed lobular panniculitis with intracellular Trypanosoma cruzi amastigotes (Fig. 1g). Qualitative (PCR) assay for T. cruzi in blood samples was positive. He was treated with benznidazole (5 mg/kg/day) for 60 days with total clearance of the lesion. Reactivation of Chagas disease is a severe complication that can occur in solid organ transplant recipients. Most cases are related to kidney or heart transplant, particularly in the first year post-transplant. Liver recipients are at lower risk due to lower doses of immunosuppressants. Indeed, we could not observe any sign of Chagas disease reactivation in our liver recipients, even in those with a diagnosis of chronic Chagas disease. It can manifest with high fever, cardiomyopathy, meningoencephalitis and several painful skin lesions. Mild systemic symptoms were observed in only one patient (case 1); however, all of them developed painful panniculitis lesions. Diagnosis can be made through parasitaemia, histopathological exam, immunohistochemistry and/or PCR. In our patients, the diagnosis was done through histopathological analysis, immunohistochemistry and qualitative PCR for T. cruzi in blood samples. Treatment can be nifurtimox or benznidazole. Our patients showed good tolerability to benznidazole with remission of lesions. In endemic areas of Chagas disease such as Latin America, Chagas disease reactivation must be considered as an important diagnosis in solid organ transplant recipients, since it can cause graft rejection and death. It also should be kept in mind in developed regions such as the United States and Europe because of the immigrants from endemic areas.
Chemical Synthesis and Biological Applications of O‐GlcNAcylated Peptides and Proteins

All human cells use O‐GlcNAc protein modifications (O‐linked N‐acetylglucosamine) to rapidly adapt to changing nutrient and stress conditions through signaling, epigenetic, and proteostasis mechanisms. A key challenge for biologists in defining precise roles for specific O‐GlcNAc sites is synthetic access to homogenous isoforms of O‐GlcNAc proteins, a result of the non‐genetically templated, transient, and heterogeneous nature of O‐GlcNAc modifications. Toward a solution, this review details the state of the art of two strategies for O‐GlcNAc protein modification: advances in “bottom‐up” O‐GlcNAc peptide synthesis and direct “top‐down” installation of O‐GlcNAc on full proteins. We also describe key applications of synthetic O‐GlcNAc peptide and protein tools as therapeutics, biophysical structure–function studies, biomarkers, and as disease mechanistic probes to advance translational O‐GlcNAc biology.
Structural basis for substrate specificity of l‐methionine decarboxylase

l ‐Methionine decarboxylase (MetDC) from Streptomyces sp. 590 is a vitamin B6‐dependent enzyme and catalyzes the non‐oxidative decarboxylation of l ‐methionine to produce 3‐methylthiopropylamine and carbon dioxide. We present here the crystal structures of the ligand‐free form of MetDC and of several enzymatic reaction intermediates. Group II amino acid decarboxylases have many residues in common around the active site but the residues surrounding the side chain of the substrate differ. Based on information obtained from the crystal structure, and mutational and biochemical experiments, we propose a key role for Gln64 in determining the substrate specificity of MetDC, and for Tyr421 as the acid catalyst that participates in protonation after the decarboxylation reaction.
The Relationship between the Family Functioning of Individuals with Drug Addiction and Relapse Tendency: A Moderated Mediation Model

To explore the relationship between family functioning, psychological capital, life history strategy, and relapse tendency of individuals with drug addiction, 842 individuals with drug addiction completed a questionnaire. The results showed that (1) there was a significant negative correlation between the family functioning of individuals with drug addiction and their relapse tendency; (2) psychological capital played an intermediary role between family functioning and relapse tendency; and (3) life history strategy regulated the mediating effect of psychological capital. The results of this study suggest that family members should collaborate with drug addiction treatment centers and participate in the education and treatment process to help reduce drug relapse tendency. Increasing the psychological capital and self-efficacy of individuals with drug addiction through group psychological counseling and psychological education courses could also reduce drug relapse tendency.
180-day readmission risk model for older adults with acute myocardial infarction: the SILVER-AMI study

Objective To develop a 180-day readmission risk model for older adults with acute myocardial infarction (AMI) that considered a broad range of clinical, demographic and age-related functional domains. Methods We used data from ComprehenSIVe Evaluation of Risk in Older Adults with AMI (SILVER-AMI), a prospective cohort study that enrolled participants aged ≥75 years with AMI from 94 US hospitals. Participants underwent an in-hospital assessment of functional impairments, including cognition, vision, hearing and mobility. Clinical variables previously shown to be associated with readmission risk were also evaluated. The outcome was 180-day readmission. From an initial list of 72 variables, we used backward selection and Bayesian model averaging to derive a risk model (N=2004) that was subsequently internally validated (N=1002). Results Of the 3006 SILVER-AMI participants discharged alive, mean age was 81.5 years, 44.4% were women and 10.5% were non-white. Within 180 days, 1222 participants (40.7%) were readmitted. The final risk model included 10 variables: history of chronic obstructive pulmonary disease, history of heart failure, initial heart rate, first diastolic blood pressure, ischaemic ECG changes, initial haemoglobin, ejection fraction, length of stay, self-reported health status and functional mobility. Model discrimination was moderate (0.68 derivation cohort, 0.65 validation cohort), with good calibration. The predicted readmission rate (derivation cohort) was 23.0% in the lowest quintile and 65.4% in the highest quintile. Conclusions Over 40% of participants in our sample experienced hospital readmission within 180 days of AMI. Our final readmission risk model included a broad range of characteristics, including functional mobility and self-reported health status, neither of which have been previously considered in 180-day risk models.
Evaluation of the selection of proper metro and tram vehicle for urban transportation by using a novel integrated MCDM approach

This paper presents a novel integrated multi-criteria decision-making model consists of the CRITIC (CRiteria Importance Through Intercriteria Correlation) technique and the EDAS (The Evaluation based on Distance from Average Solution) method to evaluate the selection of the urban rail vehicles operated in the public transport systems. In order to determine the selection criteria in a more realistic perspective, a board of experts consists of seven members, who perform as senior executives in the public transport institutions of the country, was constructed and many round tables meetings were organized with together the members of the board for determining the procedure to follow to reach meaningful and applicable results. At the beginning of the research, approximately fifty selection criteria were determined by the research team and some of them were eliminated during the preparation process of this study. Finally, 22 factors were determined as the selection criteria, which will be used in this research. These factors are the selection criteria directly or indirectly affecting the selection process and results. Also, rail tram types currently operated in the various cities of Turkey were determined as decision alternatives. These options were evaluated by using the proposed integrated MCDM approach. When the obtained results are evaluated, it can be seen that the proposed model has the potential to give very successful results for evaluating the selection process of the urban rail systems. Moreover, it can be implemented as a decision support system by decision-makers, who perform in the public transport authorities. Finally, it can also be applied for decision-making problems faced in the various fields.
Chitin Synthases Are Critical for Reproduction, Molting, and Digestion in the Salmon Louse (Lepeophtheirus salmonis)

Chitin synthase (CHS) is a large transmembrane enzyme that polymerizes Uridine diphosphate N-acetylglucosamine into chitin. The genomes of insects often encode two chitin synthases, CHS1 and CHS2. Their functional roles have been investigated in several insects: CHS1 is mainly responsible for synthesizing chitin in the cuticle and CHS2 in the midgut. Lepeophtheirus salmonis is an ectoparasitic copepod on salmonid fish, which causes significant economic losses in aquaculture. In the present study, the tissue-specific localization, expression, and functional role of L. salmonis chitin synthases, LsCHS1 and LsCHS2, were investigated. The expressions of LsCHS1 and LsCHS2 were found in oocytes, ovaries, intestine, and integument. Wheat germ agglutinin (WGA) chitin staining signals were detected in ovaries, oocytes, intestine, cuticle, and intestine in adult female L. salmonis. The functional roles of the LsCHSs were investigated using RNA interference (RNAi) to silence the expression of LsCHS1 and LsCHS2. Knockdown of LsCHS1 in pre-adult I lice resulted in lethal phenotypes with cuticle deformation and deformation of ovaries and oocytes in adult lice. RNAi knockdown of LsCHS2 in adult female L. salmonis affected digestion, damaged the gut microvilli, reduced muscular tissues around the gut, and affected offspring. The results demonstrate that both LsCHS1 and LsCHS2 are important for the survival and reproduction in L. salmonis.
TLC Bio-autography Guided Identification of Antioxidant Fraction from Aegle marmelos Rind.

The present study was devised to identify the antioxidant potential fraction from rind of Aegle marmelos. The rind extract of Aegle marmelos was analyzed by TLC bioautography. The fraction showing DPPH reduction property in TLC bioautography were further ran on preparative TLC and the positive fraction was extracted and analyzed using GC-MS. The rind of Aegle marmelos has found to have anti-oxidant compound such as Phenol 3,5-Bis (1,1-Dimethylethyl).
The Differential Heritability of Social Adjustment by Sex

Sex differences in social adjustment are frequently observed; however, there has been very little research on adaptability in the individual and social domains. The aim of this study was to investigate the sex difference in social abilities, such as high self-appeal, sociability, school adaptation, and home adaptation between school-age males and females. The sample for this study included both same-sex and opposite-sex twin pairs: a total of 467 twin pairs. We classified them into three groups: a group of those in lower classes of elementary school, a group of those in higher classes of elementary school, and a group of those in junior high school. The heritability of school adaptation was estimated to be 95% in males and 54% in females in the junior high school group. The full sex-limitation model showed a better fit in this group, and this means that a qualitative genetic difference exists. For school adaptation, there was no sex difference in lower elementary school classes; however, a quantitative difference appeared in higher classes of elementary school. Moreover, a qualitative difference appeared in junior high school. From this research, it became clear that sex differences in heritability exist for school adaptation, and there was a marked increase from the elementary school children to the junior high school children.
Energy-Efficient Hybrid Routing Protocol for IoT Communication Systems in 5G and Beyond

One of the major concerns in wireless sensor networks (WSNs) is most of the sensor nodes are powered through limited lifetime of energy-constrained batteries, which majorly affects the performance, quality, and lifetime of the network. Therefore, diverse clustering methods are proposed to improve energy efficiency of the WSNs. In the meantime, fifth-generation (5G) communications require that several Internet of Things (IoT) applications need to adopt the use of multiple-input multiple-output (MIMO) antenna systems to provide an improved capacity over multi-path channel environment. In this paper, we study a clustering technique for MIMO-based IoT communication systems to achieve energy efficiency. In particular, a novel MIMO-based energy-efficient unequal hybrid clustering (MIMO-HC) protocol is proposed for applications on the IoT in the 5G environment and beyond. Experimental analysis is conducted to assess the effectiveness of the suggested MIMO-HC protocol and compared with existing state-of-the-art research. The proposed MIMO-HC scheme achieves less energy consumption and better network lifetime compared to existing techniques. Specifically, the proposed MIMO-HC improves the network lifetime by approximately 3× as long as the first node and the final node dies as compared with the existing protocol. Moreover, the energy that cluster heads consume on the proposed MIMO-HC is 40% less than that expended in the existing protocol.
The endolysosomal adaptor PLEKHM1 is a direct target for both mTOR and MAPK pathways

The lysosome is a cellular signalling hub at the point of convergence of endocytic and autophagic pathways, where the contents are degraded and recycled. Pleckstrin homology domain‐containing family member 1 (PLEKHM1) acts as an adaptor to facilitate the fusion of endocytic and autophagic vesicles with the lysosome. However, it is unclear how PLEKHM1 function at the lysosome is controlled. Herein, we show that PLEKHM1 coprecipitates with, and is directly phosphorylated by, mTOR. Using a phosphospecific antibody against Ser432/S435 of PLEKHM1, we show that the same motif is a direct target for ERK2‐mediated phosphorylation in a growth factor‐dependent manner. This dual regulation of PLEKHM1 at a highly conserved region points to a convergence of both growth factor‐ and amino acid‐sensing pathways, placing PLEKHM1 at a critical juncture of cellular metabolism.
Feasibility of a Pilot Randomized Controlled Trial Examining a Multidimensional Intervention in Women with Gynecological Cancer at Risk of Lymphedema

There is limited knowledge on non-invasive lymphedema risk-reduction strategies for women with gynecological cancer. Understanding factors influencing the feasibility of randomized controlled trials (RCTs) can guide future research. Our objectives are to report on the design and feasibility of a pilot RCT examining a tailored multidimensional intervention in women treated for gynecological cancer at risk of lymphedema and to explore the preliminary effectiveness of the intervention on lymphedema incidence at 12 months. In this pilot single-blinded, parallel-group, multi-centre RCT, women with newly diagnosed gynecological cancer were randomized to receive post-operative compression stockings and individualized exercise education (intervention group: IG) or education on lymphedema risk-reduction alone (control group: CG). Rates of recruitment, retention and assessment completion were recorded. Intervention safety and feasibility were tracked by monitoring adverse events and adherence. Clinical outcomes were evaluated over 12 months: presence of lymphedema, circumferential and volume measures, body composition and quality of life. Fifty-one women were recruited and 36 received the assigned intervention. Rates of recruitment and 12-month retention were 47% and 78%, respectively. Two participants experienced post-operative cellulitis, prior to intervention delivery. At three and six months post-operatively, 67% and 63% of the IG used compression ≥42 h/week, while 56% engaged in ≥150 weekly minutes of moderate-vigorous exercise. The cumulative incidence of lymphedema at 12 months was 31% in the CG and 31.9% in the IG (p = 0.88). In affected participants, lymphedema developed after a median time of 3.2 months (range, 2.7–5.9) in the CG vs. 8.8 months (range, 2.9–11.8) in the IG. Conducting research trials exploring lymphedema risk-reduction strategies in gynecological cancer is feasible but challenging. A tailored intervention of compression and exercise is safe and feasible in this population and may delay the onset of lymphedema. Further research is warranted to establish the role of these strategies in reducing the risk of lymphedema for the gynecological cancer population.
Magnetic enhancement during the crystallization of ferrihydrite at 25 and 50°C

Soil formation usually results in an increase in magnetic susceptibility. The magnetic properties of the products of transformation of ferrihydrite, a typical precursor of other soil Fe oxides, were examined in the present work. Synthetic 2-line ferrihydrite was aged at two temperatures (25 and 50°C) and two different relative humidities (80 and 100%) in the presence of silicate, phosphate, citrate, and tartrate as adsorbed ligands (molar anion/Fe ratio = 1–3%). The ligands delayed or prevented the transformation of ferrihydrite to hematite. The magnetic susceptibility of the ferrihydrite transformation products increased with aging, the rate of increase depending on the type of ligand added and its concentration. The largest increase in magnetic susceptibility, sixfold, was obtained with ferrihydrite in a citrate/Fe ratio of 1%, after 1500 days. The resulting magnetic products exihibited superparamagnetic behavior at room temperature and high coercivity at 5 K. The formation of an intermediate ferrimagnetic phase in the ferrihydrite-to-hematite transformation might explain the magnetic enhancement observed in many aerobic soils lacking other sources of magnetic minerals.
Implementation of an Online Auditory Attention Detection Model with Electroencephalography in a Dichotomous Listening Experiment

Auditory attention detection (AAD) is the tracking of a sound source to which a listener is attending based on neural signals. Despite expectation for the applicability of AAD in real-life, most AAD research has been conducted on recorded electroencephalograms (EEGs), which is far from online implementation. In the present study, we attempted to propose an online AAD model and to implement it on a streaming EEG. The proposed model was devised by introducing a sliding window into the linear decoder model and was simulated using two datasets obtained from separate experiments to evaluate the feasibility. After simulation, the online model was constructed and evaluated based on the streaming EEG of an individual, acquired during a dichotomous listening experiment. Our model was able to detect the transient direction of a participant’s attention on the order of one second during the experiment and showed up to 70% average detection accuracy. We expect that the proposed online model could be applied to develop adaptive hearing aids or neurofeedback training for auditory attention and speech perception.
Towards Robust Multiple Blind Source Localization Using Source Separation and Beamforming

Multiple blind sound source localization is the key technology for a myriad of applications such as robotic navigation and indoor localization. However, existing solutions can only locate a few sound sources simultaneously due to the limitation imposed by the number of microphones in an array. To this end, this paper proposes a novel multiple blind sound source localization algorithms using Source seParation and BeamForming (SPBF). Our algorithm overcomes the limitations of existing solutions and can locate more blind sources than the number of microphones in an array. Specifically, we propose a novel microphone layout, enabling salient multiple source separation while still preserving their arrival time information. After then, we perform source localization via beamforming using each demixed source. Such a design allows minimizing mutual interference from different sound sources, thereby enabling finer AoA estimation. To further enhance localization performance, we design a new spectral weighting function that can enhance the signal-to-noise-ratio, allowing a relatively narrow beam and thus finer angle of arrival estimation. Simulation experiments under typical indoor situations demonstrate a maximum of only 4∘ even under up to 14 sources.
Albumin‐PEG‐Based Biomaterial for Laser‐Tissue Soldering and Its Real‐Time Monitoring With Swept‐Source Optical Coherence Tomography

The study presents a noninvasive, real‐time monitoring technique for the cross‐sectional imaging of the laser‐tissue soldering process with a swept‐source optical coherence tomography (SSOCT) system. The study also aims at performing a comparative study of the laser‐tissue soldering (LTS) process using optimized compositions of albumin as solder biomaterials.
Targeted Sequencing of Mitochondrial Genes Reveals Signatures of Molecular Adaptation in a Nearly Panmictic Small Pelagic Fish Species

Ongoing climatic changes, with predictable impacts on marine environmental conditions, are expected to trigger organismal responses. Recent evidence shows that, in some marine species, variation in mitochondrial genes involved in the aerobic conversion of oxygen into ATP at the cellular level correlate with gradients of sea surface temperature and gradients of dissolved oxygen. Here, we investigated the adaptive potential of the European sardine Sardina pilchardus populations offshore the Iberian Peninsula. We performed a seascape genetics approach that consisted of the high throughput sequencing of mitochondria’s ATP6, COI, CYTB and ND5 and five microsatellite loci on 96 individuals coupled with environmental information on sea surface temperature and dissolved oxygen across five sampling locations. Results show that, despite sardines forming a nearly panmictic population around Iberian Peninsula, haplotype frequency distribution can be explained by gradients of minimum sea surface temperature and dissolved oxygen. We further identified that the frequencies of the most common CYTB and ATP6 haplotypes negatively correlate with minimum sea surface temperature across the sampled area, suggestive of a signature of selection. With signatures of selection superimposed on highly connected populations, sardines may be able to follow environmental optima and shift their distribution northwards as a response to the increasing sea surface temperatures.
Danthron attenuates experimental atherosclerosis by targeting foam cell formation

What is the central question of this study? Does danthron alleviate experimental atherosclerosis by inhibiting the formation of foam cells? What are the main findings and their importance? Danthron improved serum lipid profiles and significantly reduced the atherosclerotic plaque areas and lipid accumulation in the aortic root of ApoE−/− mice. Danthron inhibited foam cell formation in oxidized low‐density lipoprotein‐induced RAW264.7 macrophages. Furthermore, danthron exerted its function in atherosclerosis at least partly through activating the AMP‐activated protein kinase–sirtuin 1 signalling pathway. These findings suggest that danthron has the potential to alleviate atherosclerosis.