Exercise Treatment Effect: The Brain Metabolite Change of Magnetic Resonance Spectroscopy in Intracerebral Haemorrhage Rat Model

Extended Abstract Intracerebral haemorrhage (ICH) consisting in an acute and spontaneous extravasation of blood into the brain parenchyma accounts for 10–15% of all stroke cases and results in the highest rates of mortality and disability of all stroke subtypes [1]. Magnetic resonance spectroscopy (MRS) has been widely used to diagnose neuro-disease. MRS is a noninvasive and in vivo method with high sensitivity and specificity [2]. The objective of this study were to examine the brain metabolite concentration quantification change by in-vivo H-MRS analysis in animal haemorrhage model, and we determined the bio-marker that was shown the effect of exercise treatment in haemorrhage disease. The 6-weeks-old 24 Sprague-Dawley rats were anaesthetized with chloral hydrate (400mg/kg, i.p.) and placed in a stereotactic frame, collagenase injected over the next 5min using a pump. The experimental group 12rats were conducted treadmill training. The training program of this research was implemented in 2 weeks with 55 to 85 percent of V02max and during determined period 15min one a day. All MRI and 1H-MRS experiments were performed on a 3.0Tesla MRI scanner (Achiva Tx 3.0 T; Philips Medical Systems, Netherlands) with a maximum gradient of 200 mT/m using a 4-channel animal coil (CG-MUC18-H300-AP, Shanghai Chenguang Medical Technologies Co., Ltd., China). A 8×6×6 mm voxel was placed within a whole brain parenchyma with hemorrhage area. The H-MRS raw data were analyzed using LCModel software (version 6.3-H, Stephen W. Provencher). The integrating areas under peaks were measured as follows: glutamate (Glu), glutamine (Gln), Choline-containing Compounds (tCho, phosphoryl choline + glycerophosphochline), N-Acetyl Aspartate (NAA), N-Acetyl Aspartyl Glutamate (NAAG). Less than 15% standard deviation (%SD) of metabolite quantification data was allowed. The %SD called the Cramer-Rao lower bound of useful reliability indicators was used for error estimates. The concentration value of (Glu+Gln)/tCr at experimental group after applying the exercise treatment was 1.838±0.606, which was significantly (p=0.021) higher than control group (1.270±0.283). Although the Glu concentration of experimental group (1.340±0.197) was higher than control group (1.153±0.132), but there was statically insignificant (p=0.067). The tCho/tCr concentration value of experimental group was 0.267±0.012, which was significantly (p=0.000) lower than control group (0.352±0.051). The NAA/tCr and NAAG/tCr of concentration could not separate, the (NAA+NAAG)/tCr concentration value for experimental and control group were 1.258±0.226 and 1.2742±0.093 respectively. No significant difference in the concentration levels of experimental and control group were observed (p=0.839). There was great significance in revealing that (Glu+Gln)/tCr value was increased, and tCho/tCr concentration level was decrease applying exercise treatment methods on hemorrhage animal model. Therefore, the metabolite concentration change of (Glu+Gln)/tCr and tCho/tCr can be used as a powerful bio-marker that represented an exercise treatment in hemorrhage patients.