Miracle-dz/UniFAR-FTUs huggingface dataset:
Online validation of combined mood induction procedures

Film clips, music, and self-referential statements (termed Velten, after their originator) have been successfully used to temporarily induce sadness and happiness. However, there is little research on the effectiveness of these procedures combined, particularly in internet-based settings, and whether Velten statements contribute to alter mood beyond the effect of simple instructions to close one's eyes and enter the targeted mood. In Study 1 (N = 106) we examined the effectiveness 80 Velten statements (positive, negative, neutral-self, neutral-facts) to create brief and effective sets that might be used in future research. In Study 2 (N = 445) we examined the effect size of 8-min combined mood induction procedures, which presented video clips in the first half and music excerpts with Velten statements or closed eyes instructions in the second half. Participants answered questionnaires on social desirability, joviality, and sadness before being randomly assigned to 1 of 7 groups varying in Valence (positive, negative, neutral) and Velten (closed eyes control, self-referential Velten, and, in the case of neutral condition, factual statements). Subsequently, participants completed the joviality and sadness scales a second time. Compared to the neutral conditions, the positive mood inductions increased joviality (Hedges G = 1.35, 95% CI [1.07, 1.63]), whereas the negative mood inductions increased sadness (Hedges G = 1.28, 95% CI [1.01, 1.55]). We did not observe any significant difference between Velten and closed eyes instructions in inducing joviality or sadness, nor did we observe any significant difference between neutral Velten statements referring to self and facts. Although social desirability bias was associated with reports of greater joviality and lower sadness, it could not account for the effects of the positive and negative mood induction procedures. We conclude that these combined mood induction procedures can be used in online research to study happy and sad mood.
Chinese firms' international expansion and performance: Based on the view of industry risk perception and enterprises' ownership as moderator

International marketing and international business researches always focus on the firms' international expansion and international performance. While, only few literatures have studied Chinese firms' expansion questions. The distinct difference between Chinese firms and west countries firms is the enterprises' ownership. We extend the international industry risk perception framework to include the unique features of the Chinese context, using empirical analysis, the result shows that (1) Industry risk perception has negative effect on the international entry mode choice; (2) The enterprises' ownership acts as a very important moderator between industry risk perception and international entry mode choice; (3) Entry mode choice heavily affects firms' international performance.
NON-INVASIVE QUANTIFICATION OF MACROPHAGIC LUNG RECRUITMENT DURING EXPERIMENTAL VENTILATION-INDUCED LUNG INJURY.

BACKGROUND Macrophagic lung infiltration is pivotal in the development of lung biotrauma due to ventilation-induced lung injury (VILI). We assessed the performance of [11C](R)-PK11195, positron emission tomography (PET) radiotracer binding the translocator protein (TSPO), to quantify macrophage lung recruitment during experimental VILI. METHODS Pigs (n=6) were mechanically ventilated under general anesthesia, using protective ventilation settings (baseline). Experimental VILI was performed by titrating tidal volume (VT) to reach a trans-pulmonary end-inspiratory pressure (∆PL) of 35-40 cmH2O. We acquired PET/computed tomography (CT) lung images at baseline, and after 4 hours of VILI. Lung macrophages were quantified in vivo by the standardized uptake value (SUV) of [11C](R)-PK11195 measured in PET on the whole lung and in 6 lung regions; and ex vivo on lung pathology at the end of experiment. Lung mechanics were extracted from CT images, to assess their association with the PET signal. RESULTS ∆PL increased from 9±1 cmH2O under protective ventilation, to 36±6 cmH2O during experimental VILI. Compared to baseline, whole lung [11C](R)-PK11195 SUV significantly increased from 1.8±0.5 to 2.9±0.5 after experimental VILI. Regional [11C](R)-PK11195 SUV was positively associated with the magnitude of macrophage recruitment in pathology (p=0.03). Compared to baseline, whole lung CT-derived dynamic strain and tidal hyperinflation increased significantly after experimental VILI, from 0.6±0 to 2.0±0.4, and 1±1 to 43±19%, respectively. On multivariate analysis, both were significantly associated with regional [11C](R)-PK11195 SUV. CONCLUSIONS [11C](R)-PK11195 lung uptake (a proxy of lung inflammation) was increased by experimental VILI, and was associated with the magnitude of dynamic strain and tidal hyperinflation.
Bundle-branch block complicating acute myocardial infarction.

Nineteen patients with right bundle-branch block (RBBB) and 20 with left bundle-branch block (LBBB) were treated in a coronary care unit for acute myocardial infarction during a 4 1/2-year period. Twenty-six percent of patients with RBBB developed cardiogenic shock, 32% had complete atrioventricular block, and 26% had ventricular asystole. Mortality was 37%, primarily from myocardial failure rather than asystole or arrhythmia. Pacemaker therapy was generally unsuccessful in reducing mortality. In contrast, 70% of the group with LBBB suffered life-threatening ventricular arrhythmia during or in close proximity to the acute phase of myocardial infarction. Mortality was 35%. Electrophysiologic mechanisms may influence ventricular irritability in patients with LBBB and ischemic heart disease. Consideration should be given to prophylactic antiarrhythmic therapy in these patients at least during hospitalization for acute myocardial infarction.
The topology of vitronectin: A complementary feature for neuroblastoma risk classification based on computer‐aided detection

Tumors are complex networks of constantly interacting elements: tumor cells, stromal cells, immune and stem cells, blood/lympathic vessels, nerve fibers and extracellular matrix components. These elements can influence their microenvironment through mechanical and physical signals to promote tumor cell growth. To get a better understanding of tumor biology, cooperation between multidisciplinary fields is needed. Diverse mathematic computations and algorithms have been designed to find prognostic targets and enhance diagnostic assessment. In this work, we use computational digital tools to study the topology of vitronectin, a glycoprotein of the extracellular matrix. Vitronectin is linked to angiogenesis and migration, two processes closely related to tumor cell spread. Here, we investigate whether the distribution of this molecule in the tumor stroma may confer mechanical properties affecting neuroblastoma aggressiveness. Combining image analysis and graph theory, we analyze different topological features that capture the organizational cues of vitronectin in histopathological images taken from human samples. We find that the Euler number and the branching of territorial vitronectin, two topological features, could allow for a more precise pretreatment risk stratification to guide treatment strategies in neuroblastoma patients. A large amount of recently synthesized VN would create migration tracks, pinpointed by both topological features, for malignant neuroblasts, so that dramatic change in the extracellular matrix would increase tumor aggressiveness and worsen patient outcomes.
Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11

One of the challenges encountered by the protein quality control machinery is the need to ensure that members of multiprotein complexes are available in the correct proportions. In this study, we demonstrate that the ubiquitin proteasome system (UPS) mediates the degradation of Apc11, the catalytic core subunit of the anaphase promoting complex/cyclosome (APC/C). In vitro studies have shown that Apc11, together with its E2 enzyme, is sufficient to ubiquitinate substrates independently of the APC/C. Here, we establish that this can occur in living yeast cells. We show that the tight controls regulating the function of the fully assembled APC/C can be circumvented when its substrates are ubiquitinated by the excess levels of Apc11 independently of the assembled complex. We thus suggest that the UPS‐mediated degradation of Apc11 is an overlooked mechanism ensuring that proper function of the APC/C is limited to suitably delimited holoenzymes and that an imbalance in protein expression may result in detrimental gain‐of‐function activity, rather than merely the disruption of protein complex stoichiometry. —Volpe, M. , Levinton, N. , Rosenstein, N. , Prag, G. , Ben‐Aroya, S. Regulation of the anaphase promoting complex/cyclosome by the degradation of its unassembled catalytic subunit, Apc11. FASEB J. 33, 9752–9761 (2019). www.fasebj. org
Conversion and reversion of anti‐John Cunningham virus antibody serostatus: A prospective study

Determination of antibodies against the John Cunningham virus (JCV) is an important tool for risk stratification in Natalizumab‐treated multiple sclerosis (MS) patients. Six‐monthly testing has been suggested for anti‐JCV antibody negative patients and patients with low antibody index in order to detect changes of serostatus. We conducted a prospective study with predefined testing intervals in order to investigate the predictability of anti‐JCV antibody status and the intervals for repetitive testing.
Acute changes in MRI diffusion, perfusion, T1, and T2 in a rat model of oligemia produced by partial occlusion of the middle cerebral artery

Oligemic regions, in which the cerebral blood flow is reduced without impaired energy metabolism, have the potential to evolve toward infarction and remain a target for therapy. The aim of this study was to investigate this oligemic region using various MRI parameters in a rat model of focal oligemia. This model has been designed specifically for remote‐controlled occlusion from outside an MRI scanner. Wistar rats underwent remote partial MCAO using an undersize 0.2 mm nylon monofilament with a bullet‐shaped tip. Cerebral blood flow (CBFASL), using an arterial spin labeling technique, the apparent diffusion coefficient of water (ADC), and the relaxation times T1 and T2 were acquired using an 8.5 T vertical magnet. Following occlusion there was a decrease in CBFASL to 35 ± 5% of baseline throughout the middle cerebral artery territory. During the entire period of the study there were no observed changes in the ADC. On occlusion, T2 rapidly decreased in both cortex and basal ganglia and then normalized to the preocclusion values. T1 values rapidly increased (within approximately 7 min) on occlusion. In conclusion, this study demonstrates the feasibility of partially occluding the middle cerebral artery to produce a large area of oligemia within the MRI scanner. In this region of oligemic flow we detect a rapid increase in T1 and decrease in T2. These changes occur before the onset of vasogenic edema. We attribute the acute change in T2 to increased amounts of deoxyhemoglobin; the mechanisms underlying the change in T1 require further investigation. Magn Reson Med 44:706–712, 2000. © 2000 Wiley‐Liss, Inc.
KEAP 1 Is a Redox Sensitive Target That Arbitrates the Opposing Radiosensitive Effects of Parthenolide in Normal and Cancer Cells

Elevated oxidative stress is observed more frequently in cancer cells than in normal cells. It is therefore expected that additional exposure to a low level of reactive oxygen species (ROS) will push cancer cells toward death, whereas normal cells might maintain redox homeostasis through adaptive antioxidant responses. We previously showed that parthenolide enhances ROS production in prostate cancer cells through activation of NADPH oxidase. The present study identifies KEAP1 as the downstream redox target that contributes to parthenolide's radiosensitization effect in prostate cancer cells. In vivo, parthenolide increases radiosensitivity of mouse xenograft tumors but protects normal prostate and bladder tissues against radiation-induced injury. Mechanistically, parthenolide increases the level of cellular ROS and causes oxidation of thioredoxin (TrX) in prostate cancer cells, leading to a TrX-dependent increase in a reduced state of KEAP1, which in turn leads to KEAP1-mediated PGAM5 and Bcl-xL (BCL2L1) degradation. In contrast, parthenolide increases oxidation of KEAP1 in normal prostate epithelial cells, leading to increased Nrf2 (NFE2L2) levels and subsequent Nrf2dependent expression of antioxidant enzymes. These results reveal a novel redox-mediated modification of KEAP1 in controlling the differential effect of parthenolide on tumor and normal cell radiosensitivity. Furthermore, they show it is possible to develop a tumor-specific radiosensitizing agent with radioprotective properties in normal cells. Cancer Res; 73(14); 1–12. 2013 AACR.
Heterogeneity in Mitogen-Activated Protein Kinase (MAPK) Pathway Activation in Uveal Melanoma With Somatic GNAQ and GNA11 Mutations

Purpose The activation of the mitogen-activated protein kinase (MAPK) pathway has been suggested as the major downstream target when GNAQ and GNA11 (GNAQ/11) are mutated in uveal melanoma (UM). However, clinical trials with single agent MEK inhibitor showed no clinical significance in altering the overall outcome of the disease in UM; therefore, we investigated the correlation between naturally occurring mutations in GNAQ/11 and activation of MAPK pathway in vivo in primary UM. Methods Screening for activating mutations in codons 183 and 209 of GNAQ/11 was carried out by sequencing and restriction fragment length polymorphism (RFLP) in a cohort of 42 primary UM. Activation of the MAPK pathway and other potential downstream signals was assessed by immunohistochemistry and/or Western blot analysis. Potential downstream signaling of mutant and wild type GNAQ/11 was studied by transient transfection assay in nonmutant cell lines. Results Somatic mutations in GNAQ/11 were observed in 35/42 (83.3%) of primary UM. Tumors with GNAQ/11 mutations showed variations in the activation of ERK1/2 with significant tumor heterogeneity. Weak and undetectable ERK1/2 activation was observed in 4/35 (11.4%) and 8/35 (22.9%) of the GNAQ/11 mutant UM, respectively. Tumor heterogeneity of GNAQ/11 mutations was also observed in a subset of tumors. Conclusions Our results indicate that there is marked variation in MAPK activation in UM with GNAQ/11 mutations. Thus, GNAQ/11 mutational status is not a sufficient biomarker to adequately predict UM patient responses to single-agent selective MEK inhibitor therapy.
Dual-Channel Reconstruction Network for Image Compressive Sensing

The existing compressive sensing (CS) reconstruction algorithms require enormous computation and reconstruction quality that is not satisfying. In this paper, we propose a novel Dual-Channel Reconstruction Network (DC-Net) module to build two CS reconstruction networks: the first one recovers an image from its traditional random under-sampling measurements (RDC-Net); the second one recovers an image from its CS measurements acquired by a fully connected measurement matrix (FDC-Net). Especially, the fully connected under-sampling method makes CS measurements represent original images more effectively. For the two proposed networks, we use a fully connected layer to recover a preliminary reconstructed image, which is a linear mapping from CS measurements to the preliminary reconstructed image. The DC-Net module is used to further improve the preliminary reconstructed image quality. In the DC-Net module, a residual block channel can improve reconstruction quality and dense block channel can expedite calculation, whose fusion can improve the reconstruction performance and reduce runtime simultaneously. Extensive experiments manifest that the two proposed networks outperform state-of-the-art CS reconstruction methods in PSNR and have excellent visual reconstruction effects.
Review on sign language recognition methods for supporting communication between deaf and non-deaf persons

With the rapid development of information and communication technology (ICT), the communication sector has improved continuously. Artificial intelligence (AI) is a field which has a broad, highly technical and specialised research area focusing on different types of sub areas and has generated a lot of interest with many novel applications in the IT world. Along with recent developments in image processing, AI has also been used to automatically recognise sign language gestures, in what is known as automatic sign language recognition (ASLR). This paper reviews methods of facilitating the communication between deaf and non-deaf persons.
Mechanisms behind Temsirolimus Resistance Causing Reactivated Growth and Invasive Behavior of Bladder Cancer Cells In Vitro

Background: Although mechanistic target of rapamycin (mTOR) inhibitors, such as temsirolimus, show promise in treating bladder cancer, acquired resistance often hampers efficacy. This study evaluates mechanisms leading to resistance. Methods: Cell growth, proliferation, cell cycle phases, and cell cycle regulating proteins were compared in temsirolimus resistant (res) and sensitive (parental—par) RT112 and UMUC3 bladder cancer cells. To evaluate invasive behavior, adhesion to vascular endothelium or to immobilized extracellular matrix proteins and chemotactic activity were examined. Integrin α and β subtypes were analyzed and blocking was done to evaluate physiologic integrin relevance. Results: Growth of RT112res could no longer be restrained by temsirolimus and was even enhanced in UMUC3res, accompanied by accumulation in the S- and G2/M-phase. Proteins of the cdk-cyclin and Akt-mTOR axis increased, whereas p19, p27, p53, and p73 decreased in resistant cells treated with low-dosed temsirolimus. Chemotactic activity of RT112res/UMUC3res was elevated following temsirolimus re-exposure, along with significant integrin α2, α3, and β1 alterations. Blocking revealed a functional switch of the integrins, driving the resistant cells from being adhesive to being highly motile. Conclusion: Temsirolimus resistance is associated with reactivation of bladder cancer growth and invasive behavior. The α2, α3, and β1 integrins could be attractive treatment targets to hinder temsirolimus resistance.
Oligonucleotide Preparation Approach for Assembly of DNA Synthons

An effective oligonucleotide preparation approach for the thermodynamically balanced, inside-out (TBIO) PCR-based assembly of long synthetic DNA molecules (synthons) is described in the current work. We replaced the necessity to purify individual oligonucleotides with just one purification procedure per approximately 500 base pairs (bp) of duplex DNA. So for an enhanced green fluorescent protein (EGFP) gene of 717 bp, we synthesized 24 oligonucleotides with a length of 50 bases and performed just two solid-phase extraction (SPE) purification procedures. It was found that the capacity of ZipTip microextractors, usually used for sample desalting in proteomics, perfectly corresponds to the gene synthesis scale (40–60 pmol). The robustness of the approach was validated with a 65-mer oligonucleotide design of the same gene. The modification of the oligonucleotide concentration gradient from the original TBIO scheme substantially increased the purity of the PCR product. We proposed a mechanism for the formation of supramolecular structures, which often occur during TBIO assembly. By using the proposed workflow, any laboratory with a standard facility for molecular biology manipulation, a 16-channel oligonucleotide synthesizer, and a conventional thermocycler has the ability to prepare one gene with a length of about 700 bp per day.
Scalable Fabrication of Porous Microchannel Nerve Guidance Scaffolds with Complex Geometries

Microchannel scaffolds accelerate nerve repair by guiding growing neuronal processes across injury sites. Although geometry, materials chemistry, stiffness, and porosity have been shown to influence nerve growth within nerve guidance scaffolds, independent tuning of these properties in a high‐throughput manner remains a challenge. Here, fiber drawing is combined with salt leaching to produce microchannels with tunable cross sections and porosity. This technique is applicable to an array of biochemically inert polymers, and it delivers hundreds of meters of porous microchannel fibers. Employing these fibers as filaments during 3D printing enables the production of microchannel scaffolds with geometries matching those of biological nerves, including branched topographies. Applied to sensory neurons, fiber‐based porous microchannels enhance growth as compared to non‐porous channels with matching materials and geometries. The combinatorial scaffold fabrication approach may advance the studies of neural regeneration and accelerate the development of nerve repair devices.
A peak power reduction scheme for wireless body area networks using UWB-IR

In this paper a peak power reduction scheme by pulse repetition for ultra-wide band (UWB) impulse radio (IR) scheme is experimentally examined for obtaining interference immunity to RF sensitive systems such as medical devices. The effectiveness of the scheme is shown in terms of an improvement of bit error rate (BER) performance.
The accuracy of drug information from pharmaceutical sales representatives.

OBJECTIVE To provide quantitative data about the accuracy of the information about drugs presented to physicians by pharmaceutical sales representatives. DESIGN One hundred six statements about drugs made during 13 presentations by pharmaceutical representatives were analyzed for accuracy. Statements were rated inaccurate if they contradicted the 1993 Physicians' Desk Reference or material quoted or handed out by the sales representative. SETTING University teaching hospital. RESULTS Twelve (11%) of 106 statements about drugs were inaccurate. All 12 inaccurate statements were favorable toward the promoted drug, whereas 39 (49%) of 79 accurate statements were favorable (P = .005). None of 15 statements about competitors' drugs were favorable, but all were accurate, significantly P < . 001) differing from statements about promoted drugs. In a survey of 27 physicians who attended these presentations, seven (26%) recalled any false statement made by a pharmaceutical representative, and 10 (37%) said information from the representatives influenced the way they prescribed drugs. CONCLUSIONS Eleven percent of the statements made by pharmaceutical representatives about drugs contradicted information readily available to them. Physicians generally failed to recognize the inaccurate statements.