Miracle-dz/UniFAR-FTUs huggingface dataset:
Sanguinarine synergistically potentiates aminoglycoside‐mediated bacterial killing

Aminoglycosides are one of the oldest classes of antimicrobials that are being used in current clinical practice, especially on multi‐drug resistant Gram‐negative pathogenic bacteria. However, the serious side effects at high dosage such as ototoxicity, neuropathy and nephrotoxicity limit their applications in clinical practice. Approaches that potentiate aminoglycoside killing could lower down their effective concentrations to a non‐toxic dosage for clinical treatment. In this research, we screened a compound library and identified sanguinarine that acts synergistically with various aminoglycosides. By checkerboard and dynamical killing assay, we found that sanguinarine effectively potentiated aminoglycoside killing on diverse bacterial pathogens, including Escherichia coli, Acinetobacter baumannii, Klebsiella pneumonia and Pseudomonas aeruginosa. The mechanistic studies showed an elevated intracellular ROS and DNA oxidative level in the bacterial cells treated by a combination of sanguinarine with aminoglycosides. Furthermore, an enhanced level of sanguinarine was observed in bacteria in the presence of aminoglycosides, suggesting that aminoglycosides promote the uptake of sanguinarine. Importantly, sanguinarine was shown to promote the elimination of persister cells and established biofilm cells both in vivo and in vitro. Our study provides a novel insight for approaches to lower down the clinical dosages of aminoglycosides.
Cambodian lending-deposit rate spread: the results of non-market influences

In the study the asymmetric adjustments in the Cambodian lending-deposit rate spread are documented. This article plans to apply Enders and Siklos (2001) procedure to test for the long-run asymmetric co-integrating relationship and Granger causality between the lending rate and the deposit rate for Cambodian, a poor developing country in Southeast Asia using monthly time series data over the period from May 1994 to June 2010. The deposit rates adjust faster when the spread is narrowing than when it is widening. These findings are consistent with the observed monopolistic/oligopolistic structure of the banking sector. The revealed predatory pricing behavior impede Cambodian industrial developments and hence slow economic developments and social progresses. The root causes of the problems are excessive government intervention and political connections, management corruptions, inefficiency and ineffectiveness. Strong political will and commitment to implement would be needed to establish a more competitive market economy for further economic growth and social progress in Cambodia.
Illegal Drug Use, Depressive Symptoms, and General Health: Exploring Co-occurrence across 11 Years in a National Sample

ABSTRACT The co-occurrence of illegal drug use, symptoms of depression, and a lower perception of general health among adolescents continues to be of substantive interest for researchers and the general public alike. Research on this topic, however, remains relatively stagnant, focusing on narrow developmental periods and each association independently, with limited consideration for the existence of a nexus between the three constructs as individuals age. Considering these limitations, the current study examines the longitudinal progression, from adolescence to early adulthood, of illegal drug use, symptoms of depression, and a lower perception of general health. The National Longitudinal Survey of Youth 1997 (NLSY97; N = 8,984), measures over an eleven-year data collection period, and between-and within-individual analytical strategies were used to evaluate the nexus between the constructs. The findings suggested that illegal drug use, depressive symptoms, and general health at previous time periods directly and indirectly predicted illegal drug use, depressive symptoms, and general health at subsequent time periods. Moreover, the within-individual change in illegal drug use was associated with the change in depressive symptoms, and the change in depressive symptoms was associated with the change in general health. Practitioners should consider this co-occurrence when treating symptoms related to illegal drug use, symptoms of depression, and physical health.
GoCo : Planning Expressive Commitment Protocols ( Article Abstract ) ?

The full article published in Autonomous Agents and MultiAgent Systems addresses the challenge of planning coordinated activities for a set of autonomous agents, who coordinate according to social commitments among themselves. The article develops a multi-agent plan in the form of a commitment protocol that allows the agents to coordinate in a flexible manner, retaining their autonomy in terms of the goals they adopt so long as their actions adhere to the commitments they have made. The setting considered allows parameterized predicates with probabilistic uncertainty over action outcomes. For this rich setting, the article contributes the first practical means to derive protocol enactments which maximise expected utility from the point of view of one agent. To do so, the article employs hierarchical planning techniques to check whether a commitment protocol can be enacted efficiently, and to generate protocol enactments under a variety of conditions. The approach is illustrated on a real-world healthcare scenario. 1 Coordination in Socio-Technical Systems The full article by Meneguzzi et al [3] recognizes that agents in an open system, although autonomous, may be interdependent in subtle ways. The physical, social, or organisational environment in which they interact can be complex. We need ways to accommodate the environment while supporting decoupling of the agents’ internals from their interaction, thus facilitating the composition of multi-agent systems. The notion of a socio-technical system (STS) provides a basis for representing such interactions between agents in the context of an organisation, and respecting technical artefacts required for the effective operation of the organisation. Activities in an STS are characterized by a combination of the goals sought by and the specified interactions between the agents. An agent’s goals relate to its social commitments at two levels: at design time, the collaborative STS design process produces a commitment protocol; and at run time, agents consider the protocol and their respective goals and make their ? This is an extended abstract of [3]. Appeared at BNAIC’18, ’s-Hertogenbosch, Netherlands, November 2018
Temper Tantrums in Toddlers and Preschoolers: Longitudinal Associations with Adjustment Problems

This article has supplementary material on the web site: www.jdbp. org. ABSTRACT: Objective: We examined parent reports of temper tantrum characteristics (e.g., frequency, duration, and behavioral profile) in toddlers and preschoolers and their longitudinal association with internalizing and externalizing adjustment problems. Methods: Parents of 1- to 5-year-olds (N = 861, Mage = 36 months, 47% girls) reported their child's temper tantrum frequency, duration, and behaviors. A subsample also reported on their child's tantrums and adjustment problems 1 year later (n = 252). We first compared the distribution of temper tantrum frequency and duration for different ages. Next, we examined which factors underlie the tantrum behaviors and whether behavioral profiles could be distinguished based on configurations of these factors within children. Finally, we performed regression analyses predicting internalizing and externalizing adjustment problems by temper tantrum frequency, duration, and behavioral profile, controlling for child sex and age. Results: Chi-square tests indicated that overall, tantrum frequency declined, whereas tantrum duration increased across the 1- to 5-year age range. We found that based on 4 tantrum behavior factors (anger, distress, aggression, and self-injurious behavior), 3 profiles characterized the tantrum behavior of children in the sample: a low-intensity profile (26%), a moderate-intensity profile (32%), and a high aggressive/self-injurious profile (42%). More frequent tantrums predicted more externalizing problems, whereas longer tantrum duration predicted internalizing problems. The high aggressive/self-injurious profile predicted adjustment problems above and beyond tantrum duration and frequency. Conclusion: Parent reports of different tantrum characteristics are uniquely predictive of different types of problems and may each be important to include in screening efforts for adjustment problems in young children.
A Near-Global Slowing of Background Activity and Epileptic Discharges in Children With Mild to Moderately Symptomatic COVID-19 Infection: An Electro-Neurophysiological Study

Background. To assess the functional involvement of the central nervous system (CNS) via quantitative electroencephalography (EEG) analysis in children with mild to moderate COVID-19 infection who were otherwise previously healthy children. Methods. This prospective, case-control study was conducted between June and September 2020. Sleep EEG records of at least 40 min were planned for children who tested positive for COVID-19 using real-time PCR analysis and within 4–6 months post-recovery. All of the EEG analyses in this study were performed on an Ubuntu 20.04. 2 LTS Operating System with the developed software using Python 3.7. 6. The quantitative analysis of the epileptic discharges within the EEG records was performed using random forest after elimination of the artifacts with a model training accuracy of 98% for each sample data point. The frequency analysis was performed using the Welch method. Results. Among the age and sex-matched groups, the global mean frequency was significantly lower among the COVID-19 patients, with a P-value of 0.004. The spike slow-wave and sharp slow-wave indices were significantly higher in the patients when compared to the controls. The mean frequency values were significantly lower in almost all of the electrodes recording the frontal, central, and occipital areas. For the temporal and parietal areas, those significantly low mean frequencies were limited to the right hemisphere. Conclusion. A near-global involvement of background activity with decreased frequency, in addition to epileptic discharges, was recorded in mild to moderately COVID-19 infected children post-infection.
When the parent imitates the child: Strategic renewal through separation and reintegration of subsidiaries

This article shows how the separation and subsequent reintegration of a subsidiary becomes a source of strategic renewal for the parent company. We develop a process model that reveals how structural ambidexterity can generate “proximate isomorphism” that gives rise to parent level exploration and the parent’s gradual convergence with the subsidiary. This creates the conditions for reintegration, and ultimately, strategic renewal. We identify the triggers for proximate isomorphism as well as the mechanisms through which it unfolds. We draw on the longitudinal analysis of strategic renewal of Immochan between 2006 and 2018. Our findings contribute to extant research by developing the link between structural ambidexterity and strategic renewal and, particularly, by showing that proximate isomorphism acts as an integrating mechanism between the parent and the explorative unit.
Adult attachment styles and negativistic beliefs about the social world: The role of self-image and other-image

This article is concerned with the relationship between adult attachment styles and generalized negativistic social beliefs (i.e. pessimistic expectations concerning human nature and interpersonal relations). Two general dimensions of attachment styles, avoidance and anxiety, are considered to be manifestations of an individual’s image of other people and of the self, respectively. We suggest that both dimensions may be a substantial basis for formulating negative beliefs about the social world. Firstly, we believe that a high level of negativistic social beliefs can be positively predicted by the growth of avoidance (negative image of others) and anxiety (negative image of self). Secondly, we formulate an expected interaction effect. Although the nature of such an interaction is ambiguous, it may be argued as having a synergistic as well as antagonistic pattern. These hypotheses were tested and supported (in favor of an antagonistic pattern of interaction in the case of the second hypothesis) on a representative sample of adult Poles (N = 853).
Rapamycin limits CD4+ T cell proliferation in simian immunodeficiency virus–infected rhesus macaques on antiretroviral therapy

Proliferation of latently infected CD4+ T cells with replication-competent proviruses is an important mechanism contributing to HIV persistence during antiretroviral therapy (ART). One approach to targeting this latent cell expansion is to inhibit mTOR, a regulatory kinase involved with cell growth, metabolism, and proliferation. Here, we determined the effects of chronic mTOR inhibition with rapamycin with or without T cell activation in SIV-infected rhesus macaques (RMs) on ART. Rapamycin perturbed the expression of multiple genes and signaling pathways important for cellular proliferation and substantially decreased the frequency of proliferating CD4+ memory T cells (TM cells) in blood and tissues. However, levels of cell-associated SIV DNA and SIV RNA were not markedly different between rapamycin-treated RMs and controls during ART. T cell activation with an anti-CD3LALA antibody induced increases in SIV RNA in plasma of RMs on rapamycin, consistent with SIV production. However, upon ART cessation, both rapamycin and CD3LALA–treated and control-treated RMs rebounded in less than 12 days, with no difference in the time to viral rebound or post-ART viral load set points. These results indicate that, while rapamycin can decrease the proliferation of CD4+ TM cells, chronic mTOR inhibition alone or in combination with T cell activation was not sufficient to disrupt the stability of the SIV reservoir.
A mixed methods end-user assessment to determine the ideal supraglottic airway device for inclusion into the medic's aid bag

What's the best airway for medics to carry in their aid bag? We sought to answer that question. Data converged around the i-gel and the BaskaMask. BACKGROUND Many advancements in supraglottic airway technology have occurred since the start of the Global War on Terrorism. While the Tactical Combat Casualty Care guidelines previously recommend the i-gel device, this is based on little data and minimal end-user input. OBJECTIVE We sought to use a mixed methods approach to investigate the properties of an ideal device for inclusion into the medic's aid bag. METHODS We performed prospective, serial qualitative studies to uncover and articulate themes relative to airway device usability with 68W-combat medics. 68W are trained roughly to the level of a civilian advanced emergency medical technician with a heavier focus on trauma care. Physicians with airway expertise demonstrated the use of each device and provided formal training on all the presented devices. We then administered performed focus groups to solicit end-user feedback along with survey data. RESULTS We enrolled 250 medics during the study. The preponderance of medics were of the rank E4 (28%) and E5 (44%). Only 35% reported ever placing a supraglottic airway in a real human. When reporting on usability, the i-gel had the highest median score, ease of manipulation, grip comfort and ease of insertion while also scoring the best in regard to requiring minimal training. The other compared devices had no clear highest score. Qualitative data saturated around a strong preference for the BaskaMask and/or the i-gel airway device, with the least favorite being the AirQ and the LMA Fastrach airway devises. There was a strong qualitative alignment in how both the BaskaMask and i-gel provided ease of use and simplicity of training. CONCLUSION There were strong qualitative preferences for two specific airway devices: Baska Mask and i-gel. However, many medics commented on their previous experience with the i-gel compared with the other devices, which may have biased them toward the i-gel. The overall data suggest that medics would prefer a device engineered with features from several devices. LEVEL OF EVIDENCE Therapeutic/care management; Level V.
Risk analysis of fast spreading species in a Kashmir Himalayan National Park (Dachigam) for better monitoring and management

Huge economic costs and ecological impacts of invasive alien species (IAS) in the protected areas (PAs) worldwide make their timely prediction and potential risk assessment of central importance for effective management. While the preborder weed risk assessment framework has been extensively evaluated and implemented, the postborder species risk assessment framework has not been subjected to the same degree of scrutiny. Here we used a rather more realistic modified version of the Australian Weed Risk framework (AWRM) for Dachigam National Park (DNP) in Kashmir Himalaya against 84 plant species, including 55 alien species and 29 fast spreading native species, for risk analysis. We found two very high‐risk species, three high‐risk species, 10 medium‐risk species, 29 low‐risk species, and 40 negligible‐risk species in the DNP. The containment scores accordingly ranged from 14.4 to 293.5 comprising of 27 species that can be contained with very high feasibility, 23 species with high feasibility, 14 species with medium feasibility, and 12 species which cannot be contained easily thereby having low feasibility of containment (FOC) score. However, eight species which have a negligible FOC score are difficult to contain within their infestation sites. Our results demonstrate the merit of the AWRM with a caution that the necessary region‐specific modifications may help in its better implementation. Overall, these results provide quite a promising tool in the hands of protected area managers to timely and effectively deal with the problem of plant invasions.
Effects of Loaded Plyometric Exercise on Post-Activation Performance Enhancement of Countermovement Jump in Sedentary Men

ABSTRACT Purpose: Explosive performance is increased right after performing loaded resistance exercise, which is known as post-activation performance enhancement (PAPE). Method: We investigated the effects of a plyometric exercise (PLYO) consisting of five sets of six drop-jumps from a 52-cm platform with a load corresponding to 20% body mass on changes in countermovement jump (CMJ) height in sedentary young men. Eleven young nonresistance trained men (19.6 ± 1.8 y, 69 ± 9 kg, 1.76 ± 0.08 m) who showed more than 4% increase in CMJ height at 4 min after five back squats with five-repetition maximum load participated in the study. Their responses to the back squat exercise were examined before (baseline) and 15 minutes, 24 and 48 hours post-PLYO. Exercise-induced muscle damage markers (maximal voluntary contraction torque [MVC], and quadriceps muscle soreness) were assessed at baseline, 15 minutes, 24 and 48 hours following PLYO. Results: MVC torque decreased (p < .05) at 15 minutes post-PLYO (−15.1 ± 9.7%) but returned to the baseline at 24 hours post-PLYO. Muscle soreness developed (p < .05) at 48 hours (21.0 ± 20.3 mm) after PLYO, indicating minor muscle damage. CMJ height increased (p < .05) after the five squats at baseline (7.6 ± 3.8%) indicating PAPE, but no such increase was found at 15 minutes, 24 and 48 hours after PLYO. However, CMJ height before the squat exercise was greater (p < .05) at 24 (5.9 ± 7.0%) and 48 hours post-PLYO (9.1 ± 8.5%) than the baseline. Conclusions: These results showed that PAPE disappeared after PLYO that induced minor muscle damage, but CMJ height increased at 24–48 hours in the recovery from PLYO exhibiting a priming effect.
A CCaMK/Cyclops response element in the promoter of L. japonicus Calcium-Binding Protein 1 (CBP1) mediates transcriptional activation in root symbioses

Early gene expression in arbuscular mycorrhiza (AM) and the nitrogen-fixing root nodule symbiosis (RNS) is governed by a shared regulatory complex. Yet many symbiosis-induced genes are specifically activated in only one of the two symbioses. The Lotus japonicus T-DNA insertion line T90, carrying a promoterless uidA (GUS) gene in the promoter of Calcium Binding Protein1 (CBP1) is exceptional as it exhibits GUS activity in both root endosymbioses. To identify the responsible cis- and trans-acting factors, we subjected deletion/modification series of CBP1 promoter:reporter fusions to transactivation and spatio-temporal expression analysis and screened EMS-mutagenized T90 populations for aberrant GUS expression. We identified one cis-regulatory element required for GUS expression in the epidermis and a second element, necessary and sufficient for transactivation by the Calcium and Calmodulin-dependent protein kinase (CCaMK) in combination with the transcription factor Cyclops and conferring gene expression during both AM and RNS. Lack of GUS expression in T90 white mutants could be traced to DNA hypermethylation detected in and around this element. We concluded that the CCaMK/Cyclops complex can contribute to at least three distinct gene expression patterns on its direct target promoters NIN (RNS), RAM1 (AM), and CBP1 (AM and RNS), calling for yet-to-be identified specificity-conferring factors.
Italian university graduates ’ transition to work

This paper explores the patterns of education-job ( mis)matching of recent university graduates, focussing on the impact of interregional migration. With the aim of offering a place-based perspective on the topic, the paper loo ks at the three Italian macro-regions of the North, the Centre and the South, comparing them wit h the country as a whole. We use an indicator of education-job (mis)matching drawn and adapted from the literature, and apply both ordered logit and probit models with self-sele ction to a dataset on graduates’ entry in the labour market produced by the Italian National Stat istical Institute. Our results suggest that, in line with most previous studies, interregional migr ation contributes to reduce education-job gaps: however, we find that the analysis for Italy as a whole masks stark differences between macro-regions, for which the typical North-South du alism still holds, confirming once more the cumulative and path-dependent nature of regiona l development trajectories.
Making the switch: The role of Gq in driving GRK selectivity at GPCRs

Selective engagement of signal transducers such as G proteins and β-arrestins with GPCRs upon stimulation with biased agonists is thought to be due to distinct receptor conformations. Kawakami et al. propose an additional mechanism whereby activation of Gq determines GPCR kinase subtype selectivity to the activated angiotensin receptor, leading to distinct binding modalities of β-arrestins and functional outcomes. Description A study reveals more evidence of the interplay between G proteins and β-arrestins in determining GPCR signaling.
Shifting Governance in Slovensky Raj National Park.

This paper explores the role of social capital and governance in rural development within Slovensky Raj National Park. Based on the theory of Common Pool Resources and Network Governance, the case study explores the external and internal influences on cooperation. Current decision making in the Park is still affected by post socialist relations. In particular inefficient institutional design and non-robust governance of the resources have resulted in over-exploitation of natural resources and treating common property as open-access. On one hand, evidence emerged on domination of interpersonal trust and failure of institutional design. These were found as barriers for the National Park to be viewed by various actors as an asset. On the other hand, municipal and tourism networks show that cooperation is gradually moving from being externally to internally driven, while displaying characteristics of bottom-up development. A hierarchical governance structure is thus slowly opening up, shifting towards networks.