Miracle-dz/UniFAR-FTUs huggingface dataset:
RhoE Regulates Actin Cytoskeleton Organization and Cell Migration

ABSTRACT The actin cytoskeleton is regulated by Rho family proteins: in fibroblasts, Rho mediates the formation of actin stress fibers, whereas Rac regulates lamellipodium formation and Cdc42 controls filopodium formation. We have cloned the mouse RhoE gene, whose product is a member of the Rho family that shares (except in one amino acid) the conserved effector domain of RhoA, RhoB, and RhoC. RhoE is able to bind GTP but does not detectably bind GDP and has low intrinsic GTPase activity compared with Rac. The role of RhoE in regulating actin organization was investigated by microinjection in Bac1.2F5 macrophages and MDCK cells. In macrophages, RhoE induced actin reorganization, leading to the formation of extensions resembling filopodia and pseudopodia. In MDCK cells, RhoE induced the complete disappearance of stress fibers, together with cell spreading. However, RhoE did not detectably affect the actin bundles that run parallel to the outer membranes of cells at the periphery of colonies, which are known to be dependent on RhoA. In addition, RhoE induced an increase in the speed of migration of hepatocyte growth factor/scatter factor-stimulated MDCK cells, in contrast to the previously reported inhibition produced by activated RhoA. The subcellular localization of RhoE at the lateral membranes of MDCK cells suggests a role in cell-cell adhesion, as has been shown for RhoA. These results suggest that RhoE may act to inhibit signalling downstream of RhoA, altering some RhoA-regulated responses, such as stress fiber formation, but not affecting others, such as peripheral actin bundle formation.
A simulation model to assess herd-level intervention strategies against E. coli O157

SUMMARY A simulation model of a herd of grazing cattle, which has been developed to provide insight into the infection dynamics of E. coli O157 is described. The spatially explicit model enables the modelling of the infection transmission processes to be realistically addressed under field management conditions. The model is used to explore the efficacy of various potential control strategies in reducing the levels of within-herd infection. These measures include restricting the size of herds, niche engineering, improving housing hygiene and vaccination. While a vaccination strategy remains a hypothetical option, it has the potential to be particularly effective. It is likely that the most successful strategy will involve the implementation of a combination of measures.
Moving toward e-government: effective strategies for increasing access and use of the internet among non-internet users in the U.S. and U.K

Increasing penetration of the Internet for government service delivery (e-government) poses new challenges for the users of those services, especially groups of people who are less likely to use the Internet but are more likely to use government services - people on low incomes, older people, and people with disabilities. Internet access in itself does not mean that those who could benefit most from government online services will use them. While there are many initiatives intended to increase access to and use of the Internet, some of which have an element of increasing use of e-government, there are few detailed accounts of what makes an initiative successful. The aim of this paper is therefore to begin to fill a gap by providing information on strategies that initiatives in the United States and the United Kingdom have used and that have been effective in terms of encouraging marginalized groups of people to use the Internet as a first step toward using e-government services. The strategies reported in this paper were identified through multiple case study research that was conducted in the U.S. and the U.K. between May and November 2006. The paper concludes with recommendations targeted at policymakers and government managers, committed to making e-government information and services universally accessible.
Sleep disordered breathing in medically stable patients with myasthenia gravis

We investigate sleep and breathing in clinically stable myasthenia gravis (MG) patients and ask weather sleep disordered breathing (SDB) is causally linked with MG. Nineteen MG patients with a mean disease duration of 9.7 years underwent sleep studies in two consecutive nights. The primary outcome measure was the respiratory disturbance index (RDI) in terms of snoring and apneas/hypopneas. Further outcome measurements were total sleep time, sleep stage distribution and the number of arousals. A clinically relevant SDB in terms of obstructive sleep apnea (OSA) (defined as RDI > 10/h) was found in four patients. There were only a few central apneas (central apnea index: 0.19 ± 0.4/h). We did not find a relationship between maximum inspiratory pressure and SDB (r = −0.03). There is no evidence for a causal relationship between medically stable MG and SDB in terms of OSA. The extent of respiratory muscle weakness failed to correlate with SDB. Furthermore, our study does not confirm the high occurrence of central respiratory events during sleep in patients with well‐controlled MG.
3D Eye Position Based Interaction within Hierarchically Represented Images

In this paper, we present a methodology on how users might interact with hierarchically represented collections of images using 3D eye position and eye blinks. The movement of a user's eye which is detected by color and circular shape information can provide a convenient, natural, and high-bandwidth source of Human-Computer Interaction for navigating and traversing up and down a hierarchical structure of an image set. Our proposed hierarchical representation scheme of an image set is composed of multiple layers by calculating the 3D position and orientation with automatic feature detection, matching and summary through feature clustering. Applications of eye tracking and hierarchical image representation can be used for view navigation within the future 3DTV system and offers an additional HCI methodology.
Acanthoplesiops cappuccino, a new species of acanthoclinine fish from the Red Sea (Teleostei: Plesiopidae) .

Acanthoplesiops cappuccino is described from the 16.4 mm SL holotype collected from Jeddah, Saudi Arabia, Red Sea. The following combination of characters distinguishes it from congeners: dorsal-fin rays XVIII,4; anal-fin rays VII,4; pectoral-fin rays 18; caudal fin not connected to last rays of dorsal and anal fins by membrane; and caudal peduncle with a pale yellowish brown bar, which extends broadly on to caudal fin. An underwater photo of the anaesthetised holotype is provided, as well as one of the freshly dead holotype and only known specimen of the similar species A. naka. Tables summarizing diagnostic characters of the six known species of Acanthoplesiops are included. congeners by possessing both rostral and maxillary barbels; having the last simple dorsal-fin ray weak and smooth; the lateral line complete, with 40-42+1 pored scales; ½7-½8/1/3½ scales in transverse line from dorsal-fin origin to pelvic-fin origin; 4½ scales between lateral line and anal-fin origin. Hypselobarbus kolus is considered a synonym of H. curmuca, which is redescribed: it is distinguished from all congeners by possessing maxillary barbels only; the last simple dorsal-fin ray weak and smooth; 41-43+1 lateral-line scales; 9-10/1/4½-5 scales in transverse line between origins of dorsal and pelvic fins; and 5½-6 scales between lateral line and anal-fin origin.
In ECAT veritas?

The laboratory criteria of the antiphospholipid syndrome include one coagulation assay (lupus anticoagulant [LA]) and two solid phase assays (anticardiolipin [aCL] and anti-ß2glycoprotein I antibodies [aß2GPI]). External quality control (EQC) surveys show that negative and clearly positive LA samples are classified correctly by about 95% of laboratories. For ‘weak’ LA there is a wide variability in samples’ classification. Furthermore, when a weak LA sample is used in two different EQC surveys more than 50% of laboratories classify it differently. In some surveys weak LA samples were found to be positive for aCL and for aß2GPI by a majority of laboratories; the main reason for laboratories which classified these samples as LA negative was a negative result in the mixing test. It is likely that, depending on the sensitivity of the assay, a weak LA cannot be detected anymore after 1:1 dilution of the sample with normal plasma. Therefore, we recommend the use of integrated assays, such as screen/confirm ratios, for the detection of weak LA samples.
Influence of Burst Failures on the H ∞ Norm

Abstract – In this work, we present an analysis of the Burst failure effe ct in theH∞ norm. We present a procedure to perform an analysis between different Markov Chain model s and a numerical example. In the numerical example the results obtained pointed out that the burst failure effe ct in the performance does not exceed 6.3%. However, this work is an introduction for a wider and more extensive analys is in this subject.
Merozoite surface protein sequence from the Camp strain of the human malaria parasite Plasmodium falciparum.

We previously reported the sequence of the 5' portion of the genomic DNA encoding a major merozoite surface protein from the Plasmodium falciparum Camp strain (1). Subsequently, overlapping Camp strain size-selected genomic DNA fragments containing the remaining 3' portion of the gene were cloned. Below is shown the sequence for this part of the gene with continuation in numbering. The new is very to the analogous sequence from the MAD20 strain (2), previous conclusions about dimorphism and intragenic recombination (1,2).
FPGA based Ternary Content Addressable Memory using SRAM

Ternary content addressable memory, TCAM is a semiconductor memory device, operates for determining a match between received search data and stored data in TCAM bitcells. TCAM performs high speed lookup operations, but when compared to RAM technology the conventional TCAM circuitry suffers from certain limitations such as low access time, low storage capacity, circuit complexity and much high cost. So, here TCAM cells are combined with SRAM to emulate itsfunctionality. A8X4 design has been implemented using vertical partitioning and hybrid partitioning of conventional TCAM table in Xillinx Virtex-2 FPGA. After analysis, it is finding that the results of VP-SRAM based TCAM are better in terms of area and maximum combinational path delay.
Achieving energy efficiency through load balancing: A comparison through formal verification of two WSN routing protocols

Network lifetime is one of the most crucial factors that affect the performance of a Wireless Sensor Network (WSN). In order to maximize the network lifetime, it is essential that a routing protocol is designed from the purpose of energy efficiency. One way through which energy efficiency can be achieved in a protocol is by evenly distributing load among all the nodes in the network. This paper introduces Robust Formally Analysed Protocol for Wireless Sensor Networks Deployment with Load Balancing (RAEED-LB). This protocol takes the decision of selecting the next node on the basis of load balancing. The performance of RAEED-LB and RAEED is compared through formal verification. The formal verification results show that RAEED-LB achieves network lifetime gain in the range of 10% to 35% over RAEED.
Long-term restoration of visual function in end-stage retinal degeneration using subretinal human melanopsin gene therapy

Significance Inherited retinal degenerations may result in blindness due to a progressive loss of photoreceptor cells. We assess subretinal delivery of human melanopsin using an adeno-associated viral vector to remaining retinal cells in a model of end-stage retinal degeneration. Human melanopsin, being already present in the eye, is unlikely to generate an immune response when introduced via gene therapy. Furthermore, this method of delivery has been proven to be safe in clinical trials and may be more effective at delivering vector in primates than the alternative method of intravitreal injection. We demonstrate long-term vector expression and restoration of visual function, indicating that this therapy could be stable and efficacious in the treatment of patients with end-stage retinal degenerations. Optogenetic strategies to restore vision in patients who are blind from end-stage retinal degenerations aim to render remaining retinal cells light sensitive once photoreceptors are lost. Here, we assessed long-term functional outcomes following subretinal delivery of the human melanopsin gene (OPN4) in the rd1 mouse model of retinal degeneration using an adeno-associated viral vector. Ectopic expression of OPN4 using a ubiquitous promoter resulted in cellular depolarization and ganglion cell action potential firing. Restoration of the pupil light reflex, behavioral light avoidance, and the ability to perform a task requiring basic image recognition were restored up to 13 mo following injection. These data suggest that melanopsin gene therapy via a subretinal route may be a viable and stable therapeutic option for the treatment of end-stage retinal degeneration in humans.
Electronic structures of lanthanum, samarium, and gadolinium sulfides

In this study, we report our efforts to elucidate the electronic structures of two lattice structures of lanthanide sulfides (LnS and Ln3S4) and for three lanthanides (Ln = La, Sm and Gd) using density functional theory calculations performed with the CASTEP code. A DFT+U method was used for the corrections of on-site Coulomb interactions with U = 6 eV. The calculated electronic structures show that both lanthanum and gadolinium sulfides have metallic properties, consistent with the available experimental results. However, the calculated electronic structure of Sm3S4 is considerably different from those of the La 3 S 4 and Gd 3 S 4 and is predicted to have semiconducting properties.
A reinforcement learning approach to dynamic optimization of load allocation in AGC system

A Reinforcement Learning (RL) method applied to the dynamic load allocation in AGC system is presented. The problem can be modeled as a Markov Decision Process (MDP). The Q-learning algorithm as a model-free learning algorithm is introduced. It learns an optimal action strategy by experience from exploring an unknown system and getting rewards. Rewards are chosen to express how well actions control the system. The applications of the Q-learning algorithm to the two-area power system model and China Southern Power Grid model are presented. The case study shows that the Q-learning algorithm enhances the performance of AGC system under CPS.
3D Human Action Recognition using Hu Moment Invariants and Euclidean Distance Classifier

This paper presents a new model of scale, rotation, and translations invariant interest point descriptor for human actions recognition. The descriptor, HMIV (Hu Moment Invariants on Videos) is used for solving surveillance camera recording problems under different conditions of side, position, direction and illumination. The proposed approach deals with raw input human action video sequences. Seven Hu moments are computed for extracting human action features and for storing them in a 1D vector which is constringed as one mean value for all the frames’ moments. The moments are invariant to scale, translation, or rotation, which is the robustness point of Hu moments algorithm. The experiments are evaluated using two different datasets; KTH and UCF101. The classification process is executed by calculating the Euclidean distance between the training and testing datasets. Human action with minimum distance will be selected as the winner matching action. The maximum classification accuracy in this work is 93.4% for KTH dataset and 92.11% for UCF101.
Constructing on-line knowledge sharing model for R&D staff: An empirical study in Taiwan

The number of enterprises implementing knowledge management (KM) has grown dramatically in recent years. However, few companies could achieve their target and successfully implement KM, especially in Taiwan. There are several factors that need to be considered while developing or implementing knowledge sharing system. This paper is intended to specify factors which effect knowledge sharing as perceived by R&D engineers. The study conducted a questionnaire survey along with literature review. Confirmatory factor analysis was used to assess the fitness between the model and data. Based on the results, two conclusions were made as follows. First, six aspects composed factors affecting one to share knowledge on-line, which were organizational culture, base construction of discussion board, rewards, job stress, knowledge sharing design, and willingness of sharing knowledge. Second, among the six aspects, the factor of knowledge sharing design influenced most, and then organizational culture, rewards, base construction of discussion board, job stress, and willingness of sharing knowledge orderly.
Reliable guaranteed cost control for uncertain nonlinear systems

This paper deals with the reliable control design problem for uncertain nonlinear systems. A more practical model of actuator failures than outage is adopted. Based on the Hamilton-Jacobi inequality approach from nonlinear H/sub /spl infin// control theory, a method for designing reliable state feedback controllers is presented. The resulting control systems are robustly stable and with an H/sub 2/ performance bound against plant uncertainty and actuator failures.
A Bayesian approach to two-mode clustering

We develop a new Bayesian approach to estimate the parameters of a latent-class model for the joint clustering of both modes of two-mode data matrices. Posterior results are obtained using a Gibbs sampler with data augmentation. Our Bayesian approach has three advantages over existing methods. First, we are able to do statistical inference on the model parameters, which would not be possible using frequentist estimation procedures. In addition, the Bayesian approach allows us to provide statistical criteria for determining the optimal numbers of clusters. Finally, our Gibbs sampler has fewer problems with local optima in the likelihood function and empty classes than the EM algorithm used in a frequentist approach. We apply the Bayesian estimation method of the latent-class two-mode clustering model to two empirical data sets. The first data set is the Supreme Court voting data set of Doreian, Batagelj, and Ferligoj (2004). The second data set comprises the roll call votes of the United States House of Representatives in 2007. For both data sets, we show how two-mode clustering can provide useful insights.
Each of the conserved sequence elements flanking the cleavage site of mammalian histone pre-mRNAs has a distinct role in the 3'-end processing reaction

To study the substrate requirements for the histone 3'-end processing reaction of mammalian histone pre-mRNAs, we created a set of mutations in the sequences flanking the processing site of a mouse H3 gene. We found that deletion of the downstream purine-rich element hypothesized to interact with U7 small nuclear RNA abolishes in vitro 3'-end processing. Somewhat surprisingly, however, mutations in the hairpin loop element which destabilize or destroy the secondary structure reduce but do not abolish 3'-end processing. This is in apparent contrast to results obtained for the sea urchin system, where both sequence elements appear to be absolutely required for 3'-end formation.
Anti‐Listeria effect of enterocin A, produced by cheese‐isolated Enterococcus faecium EFM01, relative to other bacteriocins from lactic acid bacteria

Enterocin A produced by Enterococcus faecium EFM01 displayed a narrow antimicrobial spectrum, mainly directed against Listeria spp. In particular, the bacteriocin was extremely active against 13 Listeria monocytogenes strains. This high specificity of action of enterocin A for Listeria spp. relative to lactic acid bacteria, together with its broad range of activity from pH 4·0 to pH 9·0, are factors which may be of great interest with respect to the potential antilisterial use of this bacteriocin in fermented foods. Assessment of the effect of enterocin A concentration on the extent and kinetics of bactericidal activity on L. monocytogenes Lm 6 (107 cfu ml−1 in culture broth), suggested that viability losses of higher than 5 log10, and time intervals necessary for maximum loss of viability of less than 2 h, could not be obtained. Moreover, it was shown that both parameters are closely dependent on the Listeria strain used. On the other hand, at concentrations inducing destruction of approximately 2 log10 cycles, maximum loss of viability was achieved within time intervals which varied widely from one lactic acid bacteria bacteriocin to another.
Contribution of local renin-angiotensin system to cardiac hypertrophy, phenotypic modulation, and remodeling in TGR (mRen2)27 transgenic rats.

BACKGROUND The transgenic rat TGR(mRen2)27, carrying the mouse Ren-2 gene, is a new model to elucidate the role of the local renin-angiotensin system in vivo. However, the role of the local renin-angiotensin system in the heart remains to be determined in TGR(mRen2)27. METHODS AND RESULTS TGR(mRen2)27 were treated with various antihypertensive drugs for 6 weeks to examine the effects on cardiac hypertrophy and gene expression. Cardiac mRNAs were examined by Northern blot analysis. In TGR(mRen2)27, left ventricular hypertrophy was associated with a decrease in alpha-myosin heavy chain expression of 31% and an increase in skeletal alpha-actin and atrial natriuretic polypeptide expression by 2.6- and 21-fold, respectively (P < .05), thereby showing the shift of myocardium to a fetal phenotype. Furthermore, cardiac collagen and laminin expressions were increased in TGR(mRen2)27 (P < .05), suggesting the occurrence of cardiac remodeling. Although treatment of TGR(mRen2)27 with a high dose of TCV-116 (angiotensin AT1 receptor antagonist) or manidipine (calcium antagonist) combined with atenolol (beta 1-adrenergic receptor blocker) completely normalized blood pressure, TCV-116 regressed cardiac hypertrophy and suppressed the changes in cardiac mRNA levels of TGR(mRen2)27 much more potently than manidipine with atenolol. Furthermore, the inhibitory effects of a low dose of TCV-116 on cardiac hypertrophy and altered gene expressions of TGR(mRen2)27 were greater than those of doxazosin (alpha 1-adrenergic receptor blocker) combined with atenolol, despite their similar hypotensive effects. CONCLUSIONS Our present observations provide evidence that the cardiac renin-angiotensin system in TGR(mRen2)27 is responsible for cardiac hypertrophy, phenotypic modulation, and remodeling.
Maximum Entropy Spectral estimation based on accelerating genetic algorithm

The purpose of this paper was to solve the problems of spectral peak shifting and line splitting existing in Burg's Maximum Entropy Spectral Analysis method (MESA), to enhance the resolution of entropy spectral, and to increase the adaptability of spectral estimation algorithm to signal length, signal noise ratio and initial phase. A method of accelerating Genetic algorithm based maximum Entropy Spectral estimation method (GES) was proposed, where accelerating genetic algorithm was used to optimize the parameters of MESA and the four equivalent conditions of MESA were used as objective function. Three typical simulation cases indicated that the phenomenon of spectral peak shifting and line splitting were absent in the frequency spectral estimated by GES, and the ability to discriminate two closed frequency was improved. Compared with the traditional MESA methods, GES has good performances in signal processing.