Miracle-dz/UniFAR-FTUs huggingface dataset:
The biochemical characterization of the DNA binding activity of pKi67

Ki67 is only expressed in the nucleus of cycling cells. While it is employed as an operational marker of proliferation, little is known of the biochemical properties of this large protein. Using an immunoaffinity strategy for purification of pKi67, this study has shown that it can form higher‐order complexes and can bind to DNA cellulose in vitro. No other co‐purifying proteins could be identified, strongly suggesting that the DNA binding activity is an inherent property of pKi67. Using an electromobility shift assay, the affinity of pKi67 was shown using a range of different forms of DNA as competitors. Single‐stranded DNA was the poorest competitor, followed by double‐stranded DNA, with supercoiled DNA being the best competitor. In addition, it was found that purified pKi67 has a preference for AT‐rich DNA. The DNA binding domain is mapped to the C‐terminal domain of pKi67, and recombinant protein from the terminal 321 residues of pKi67 can bind DNA in vitro. GFP constructs from this domain were used to map regions that could target nucleolar localization and allow DNA binding. Finally, it was found that over‐expression of the C‐terminal 321 residues in cells induced chromatin disruption and apoptosis. These data provide strong evidence that pKi67 has a novel DNA binding activity within the C‐terminal domain and that this protein can influence chromatin structure. Copyright © 2000 John Wiley & Sons, Ltd.
Composition and Larvicidal Activity of Artemisia vulgaris L. Stem Essential Oil Against Aedes aegypti

This study investigates the composition and potential larvicidal activity of the essential oils isolated from the elite plant, Artemisia vulgaris L. which is commonly known as mugwort. Essential oils were hydrodistilled from in vitro raised stems and analysed by GC-MS. The major components of this oil were camphor, camphene, α-thujone, 1,8-cineole, γ-muurolene and β-caryophyllene. Mosquito larvicidal assays were carried out to test the efficacy of the oil against the dengue vector, Aedes aegypti. Laboratory reared third instar larvae were exposed to different concentrations of the oil solution and activity was assessed at different exposure times according to standard WHO procedure. Results showed that 100% larval mortality was achieved when the larvae were treated with 500 ppm oil solution with an exposure time of 8 h. The present study has shown that mugwort oil is a potential larvicide against Aedes aegypti even in low doses of the test solution. The results indicate that the natural insecticides could be used in the place of synthetic insecticides and save our environment from chemical hazards.
Document representation methods for clustering bilingual documents

Globalization places people in a multilingual environment. There is a growing number of users to access and share information in several languages for public or private purpose. In order to deliver relevant information in different languages, efficient multilingual documents management is worthy of study. Generally, classification and clustering are two typical methods for documents management. However, lack of training data and high efforts for corpus annotation will increase the cost for classifying multilingual documents which needs to bridge language gaps as well. Clustering is more suitable to implement in such practical applications. There are two main factors involved in documents clustering, document representation method and clustering algorithm. In this paper, we focus on document representation method and demonstrate that the choice of representation methods has impacts on quality of clustering results. In our experiment, we use parallel corpora (English‐Chinese documents on topic of technology information) and comparable corpora (English and Chinese documents on topics of mobile technology and wind energy) as dataset. We compare four different types of document representation methods: Vector Space Model, Latent Semantic Indexing, Latent Dirichlet Allocation and Doc2Vec. Experimental results show that, accuracy of Vector Space Model were not competitive with other methods in all clustering tasks. Latent Semantic Indexing is overly sensitive to corpora itself, for it behaved differently when clustering two different topics of comparable corpora. Latent Dirichlet Allocation behaves best when clustering documents in small size of comparable corpora while Doc2Vec behaves best for large documents set of parallel corpora. Accordingly, characteristics of corpora should be under considerations for rational utilization of document representation methods to have better performance.
Influence of dietary protein and fat on serum lipids and metabolism of essential fatty acids in rats

A 120 d feeding study with adult rats was conducted to evaluate the influence of two protein sources (casein and gelatin), two protein levels (50 and 300g/kg diet) and two fat levels (50 and 150g/kg diet) on serum lipids (total cholesterol, HDL-cholesterol and triacylglycerols) and liver polyunsaturated fatty acid levels. In general, the concentrations of serum triacylglycerols and total cholesterol and liver phospholipid levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) were higher in rats fed on casein diets compared with those fed on the gelatin diets. These effects were more pronounced in rats fed on the high-casein (300 g/kg)-high-fat (150g/kg) diet. Gelatin was hypocholesterolaemic and also suppressed the liver phospholipid levels of AA and DHA (reported for the first time). The difference in the amino acid composition between casein and gelatin may be responsible for the observed effects. Casein contains higher levels of glutamic acid, methionine, phenylalanine and tyrosine, while gelatin contains higher levels of arginine, glycine and hydroxyproline. It is suggested that a protein source which increases serum cholesterol may also increase the concentrations of AA and DHA in rat tissues.
On the location of plumes and lateral movement of thermochemical structures with high bulk modulus in the 3‐D compressible mantle

The two large low shear velocity provinces (LLSVPs) at the base of the lower mantle are prominent features in all shear wave tomography models. Various lines of evidence suggest that the LLSVPs are thermochemical and are stable on the order of hundreds of million years. Hot spots and large igneous province eruption sites tend to cluster around the edges of LLSVPs. With 3‐D global spherical dynamic models, we investigate the location of plumes and lateral movement of chemical structures, which are composed of dense, high bulk modulus material. With reasonable values of bulk modulus and density anomalies, we find that the anomalous material forms dome‐like structures with steep edges, which can survive for billions of years before being entrained. We find that more plumes occur near the edges, rather than on top, of the chemical domes. Moreover, plumes near the edges of domes have higher temperatures than those atop the domes. We find that the location of the downwelling region (subduction) controls the direction and speed of the lateral movement of domes. Domes tend to move away from subduction zones. The domes could remain relatively stationary when distant from subduction but would migrate rapidly when a new subduction zone initiates above. Generally, we find that a segment of a dome edge can be stationary for 200 million years, while other segments have rapid lateral movement. In the presence of time‐dependent subduction, the computations suggest that maintaining the lateral fixity of the LLSVPs at the core‐mantle boundary for longer than hundreds of million years is a challenge.
Streptococcus anginosus infection in oral cancer and its infection route.

OBJECTIVE To elucidate a possible involvement of Streptococcus anginosus in oral cancer, we assessed the frequency of S. anginosus infection in oral cancer tissues, and investigated its infection route. MATERIALS AND METHOD The tissue specimens were obtained from 46 oral cancer and three precancerous leukoplakia subjects. Frequency of S. anginosus infection was assessed by a species-specific polymerase chain reaction (PCR) assay. The genotype of the clinical isolates taken from cancer tissue and dental plaque samples was analyzed using pulsed-field gel electrophoresis (PFGE). RESULTS S. anginosus DNA was frequently detected in squamous cell carcinoma (19/42), but not in other types of cancer (lymphoma and rhabdomyosarcoma) or leukoplakia samples. A subject-based analysis revealed that S. anginosus was solely detected in dental plaque and not in saliva from all 19 S. anginosus-positive squamous cell carcinoma cases. Further, the genotype of S. anginosus isolated from cancer tissue was identical to that from dental plaque of the same patients. CONCLUSION Infection of S. anginosus could occur frequently in oral squamous cell carcinoma and that dental plaque could be a dominant reservoir of the S. anginosus.
The Team Approach to Home‐Based Primary Care: Restructuring Care to Meet Individual, Program, and System Needs

Team‐based models of care are an important way to meet the complex medical and psychosocial needs of the homebound. As part of a quality improvement project to address individual, program, and system needs, a portion of a large, physician‐led academic home‐based primary care practice was restructured into a team‐based model. With support from an office‐based nurse practitioner, a dedicated social worker, and a dedicated administrative assistant, physicians were able to care for a larger number of patients. Hospitalizations, readmissions, and patient satisfaction remained the same while physician panel size increased and physician satisfaction improved. The Team Approach is an innovative way to improve interdisciplinary, team‐based care through practice restructuring and serves as an example of how other practices can approach the complex task of caring for the homebound.
Passive Radar Array With Low-Power Satellite Illuminators Based on Fractional Fourier Transform

This paper addresses the detection of surface slow moving targets by means of an array passive radar based on low-EIRP satellite illuminators of opportunity. Particularly, the use of the receiving array of K elements is considered to provide increased detection capability for targets with limited radar cross section, together with the capability to estimate the target direction-of-arrival. A full processing scheme is proposed to exploit together both the long integration times and the multiple receiving elements. This includes a coherent space-time integration of the signal received at the K antenna elements during short frames, followed by an incoherent frame to frame integration. The proposed scheme is based on the fractional Fourier transform that is shown to map the signals into a natural rotated frequency-angle plane, where we can easily compensate the target Doppler frequency shifts available at the different antenna elements and temporal frames. Its effectiveness is shown by application to different synthetic scenarios, where the target detection is only achievable when all the possible integration gain is capitalized.
Statistical Video Based Control of Mobile Robots

A typical approach to the control of mobile robots is based on the analysis of signals from various kinds of sensors (e.g. infra-red or ultrasound). Another type of input data used for motion control of robots can be video signals acquired by the cameras. In such case many algorithms can be utilised e.g. SLAM (Simultaneous Localization and Mapping) and its modifications, being still an active field of research (Kalyan et al., 2010), which require usually the full analysis of the images (or video frames) being the input data (Se et al., 2001; 2002), in some applications using also additional operations such as Principal Component Analysis (Tamimi & Zell, 2004). Similar problems may also occur in the robot localisation and map building processes (Hahnel et al., 2003). An interesting alternative can be the application of the fast image analysis techniques based on the statistical experiments. Presented statistical approach is related to the first step of such control systems, which can be considered as the pre-processing technique, reducing the amount of data for further analysis with similar control accuracy. Discussed methods can be utilised for many control algorithms based on the image and video data e.g. the proportional steering of line following robots (Cowan et al., 2003) considered as a representative example in further discussions. Nevertheless, some other applications of the presented approach are also possible e.g. fast object classification based on the shape analysis (Okarma & Lech, 2009) or motion detection, as well as fast reduced-reference image quality estimation (Okarma & Lech, 2008b).
Circulating Osteoprotegerin Affects Bone Metabolism in Dialysis Patients With Mild Secondary Hyperparathyroidism

Abstract: Osteoprotegerin (OPG) is a soluble glycoprotein which inhibits osteoclastic formation and activity. Circulating OPG levels are elevated in uremia. The role of elevated circulating OPG levels in uremia remains unknown. Blood samples were obtained from 22 non‐diabetic dialysis patients who underwent iliac bone biopsy examination. The serum OPG concentration was assayed by ELISA. The circulating OPG levels showed a negative correlation with the ratio of eroded surface/bone surface (ES/BS) in biopsied iliac bone samples among 15 of those with plasma intact PTH levels less than 300 pg/mL (P < 0.05, r2 = 0.270). Patients with serum OPG levels less than 2.0 ng/mL showed significantly greater ES/BS values than those with levels ≥3.0 ng/mL, while the intact PTH levels were comparable among those groups. These tendencies disappeared when seven patients with plasma intact PTH levels more than 300 pg/mL were included into the analysis. In conclusion, circulating OPG levels showed a significant negative correlation with a bone resorption parameters in dialysis patients with mild secondary hyperparathyroidism. Circulating OPG might have a suppressive effect on osteoclastic bone resorption in dialysis patients.
Set Theory Correlation Free Algorithm for HRRR Target Tracking

Abstract : One challenge of simultaneous tracking and identification of targets is the fusion of continuous and discrete information. Recently a few fusionists including Mahler 1 and Mori 2 are using a set theory approach for a unified data fusion theory which is a correlation free paradigm 3 This paper uses the set theory approach as a basis for a method of fusing kinematic-continuous data and identification-discrete feature information. The set of features are high range resolution radar range-bin locations and amplitudes which are collected over a small aperture and a scrambled method is used to order a feature set. Once features are ordered, a recursive belief filter operates in feature space to combine track and identification measurements. The intersection of track and identification methods results in a simultaneous tracking and identification algorithm which accumulates evidence for belief in targets and rules out non-plausible targets.
An introduction to biostatistics.

This introduction to biostatistics and measurement is the first in a series of articles designed to provide Radiology readers with a basic understanding of statistical concepts. Although most readers of the radiology literature know that application of study results to their practice requires an understanding of statistical issues, many may not be fully conversant with how to interpret statistics. The goal of this series is to enhance the ability of radiologists to evaluate the literature competently and critically, not make them into statisticians.
Conceptualizing and measuring generic health outcomes

T IS A PLEASURE and honor to have the opportunity to I address this conference again. My research does not focus on the cancer field and, in that sense, I am an outsider looking in. I have followed your progress in conceptualizing and measuring health and quality of life in studies of cancer with great interest. In addition to the progress you and the American Cancer Society (ACS) have made in the cancer field, you have helped move medicine toward greater understanding and acceptance of the importance of the patient point of view. I realize that in stressing the importance of monitoring the patient perspective regarding the impact of disease and treatment, I will be “preaching to the converted. ” However, I want to take this opportunity to push this field even further in directions in which I think it should go and in which we are now more capable of going. 1 plan to take a very broad perspective, broader than on research alone. 1 will consider issues of public policy, the routine monitoring of the outcomes of medical care from the patient point of view in managing health care systems, and the routine use of standardized patient health surveys in everyday medical practice. As a point of departure in preparing for this conference, I reviewed my comments at the 1983 ACS Methods Workshop. ’ At that time I emphasized the “standardized patient survey” as a method of data collection. Part of that emphasis included recognizing the importance-and
Improved transient response of controllers by synchronizing the modulator with the load step: application to v2ic

V2Ic is a ripple-based control with an excellent performance for load transients and reference voltage tracking because it exhibits a feedforward of the load current and the error of the output voltage. However, if V2Ic is modulated with constant frequency, constant on-time or constant off-time, its dynamic response is hindered by delays in the response. This paper proposes a technique that synchronizes the clock of the converter to initialize the duty cycle when a worst-case load transient occurs using the current through the output capacitor to detect load transients. It is exemplified on a V2Ic control but it is applicable to most of controllers as it only acts on the modulator.
Multislice CT Evaluation of Airway Stents

Tracheobronchial stents are playing an increasing role in the palliative treatment of large airway obstruction due to a variety of conditions, including extrinsic compression, intraluminal disease, and malacia. Computed tomography (CT) plays an important role in aiding planning of stent placement and in detecting various stent complications. In this pictorial essay, we illustrate and review the role of multislice CT in the pre- and post-stent placement settings. A special emphasis is placed upon the characteristic CT appearance of specific stent complications and upon the role of CT multiplanar reformations and 3-dimensional (3-d) reconstruction techniques.
Properties of an ordered ring structure formed by recombinant Treponema pallidum surface antigen 4D

Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-treated 4D is an ordered ring structure of 6-nm diameter. Evidence is presented that the 190-kDa ordered ring is maintained by noncovalent bonds; 19-kDa monomers can reassociate in vitro to reform a 190-kDa molecule. Amino acid composition analysis of 190-kDa 4D showed that the molecule is composed of 45% hydrophobic residues. Evidence relating the structure of the 4D ordered ring to its potential role in the pathogenesis of syphilis is discussed.
Effects of Dietary Wild-ginseng Adventitious Root Meal on Growth Performance, Blood Profiles, Relative Organ Weight and Meat Quality in Broiler Chickens

【This experiment was conducted to evaluate the effects of dietary wild-ginseng adventitious root meal (WGM) on growth performance, blood profiles, relative organ weight and meat quality of broiler chickens. A total of 480, 2-day-old male broiler chicks (BW = $42.8{\pm}1.38\;g$ ) were randomly allocated to 1 of 4 dietary treatments (6 cages with 20 broilers per cage). Dietary treatments were: i) CON (basal diet), ii) WGM0.1 (basal diet+0.1% WGM), iii) WGM0.2 (basal diet+0.2% WGM) and iv) WGM0.3 (basal diet+ 0.3% WGM). Birds fed WGM0.3 diet (p】
A Study of Perceived Organizational Justice, Trust, and Organisational Citizenship Behaviour

The present organizations in which cut throat competition is there among employees, organizational justice and trust become more important to develop organizational citizenship behavior. organisation citizenship behavior is voluntary behavior on the part of employee which does not bring any reward to them. An employee who feels like citizen of organization does something extra for organization and coworkers which is not described in job description. Many previous researches proved impact of trust on organizational citizenship behavior and impact of organizational justice on organizational citizenship behavior. This study attempts to find out impact of trust and justice on OCB separately as well as combined impact of both the variables on OCB in service industry of Gwalior (MP, India). various statistical tools like reliability analysis and regression analysis was applied to the gathered data to fulfill the objective of the study.
The Improvement of the Processes of a Class of Graph-Cut-Based Image Segmentation Algorithms

In this paper, an analysis of the basic process of a class of interactive-graph-cut-based image segmentation algorithms indicates that it is unnecessary to construct n-links for all adjacent pixel nodes of an image before calculating the maximum flow and the minimal cuts. There are many pixel nodes for which it is not necessary to construct n-links at all. Based on this, we propose a new algorithm for the dynamic construction of all necessary n-links that connect the pixel nodes explored by the maximum flow algorithm. These n-links are constructed dynamically and without redundancy during the process of calculating the maximum flow. The Berkeley segmentation dataset benchmark is used to prove that this method can reduce the average running time of segmentation algorithms on the premise of correct segmentation results. This improvement can also be applied to any segmentation algorithm based on graph cuts. key words: graph cut, image segmentation, energy function, maximum flow
SPERM DNA FRAGMENTATION IS INCREASED IN COUPLES WITH UNEXPLAINED RECURRENT PREGNANCY LOSS

Previous studies have indicated that sperm quality may be related to unexplained recurrent pregnancy loss. This study evaluated the degree of sperm DNA fragmentation using the TUNEL assay on sperm from 24 couples with unexplained recurrent pregnancy loss (RPL) compared to sperm from 2 control groups: donors of known fertility and unscreened men from the general population. The percentage of sperm staining positive for DNA fragmentation was increased ( p <.001) in the RPL group (38±4.2) compared to the donor (11.9±1.0) or general population (22±2.0) control groups. In the RPL group, no correlation was observed between semen quality parameters and the TUNEL data. These data indicate that some RPL patients have a significant increase of sperm DNA fragmentation, which may be causative of pregnancy loss in some patients.
Ovulation induced by gonadotrophins in the marsupial, Sminthopsis crassicaudata (Gould).

Summary. Injection of hcg into mature, female S. crassicaudata induced ovulation in three of six animals, whereas injection of pmsg or of pmsg followed after 48 hr by hcg induced ovulation in all of seventeen females. Seven mated but none littered. Ten adult females were injected with pmsg and hcg at the end of an induced oestrous cycle, when the corpora lutea were degenerate. Six mated but none littered. Of eight young females injected with pmsg and hcg, four mated but again none littered. All ova recovered after induced ovulation were unfertilized. The number of ova released at induced ovulation was higher than in untreated animals and many unruptured follicles became luteinized. Ova were retained in the oviducts for longer than normal.