Miracle-dz/UniFAR-FTUs huggingface dataset:
Mild hearing loss in C57BL6/J mice after exposure to antiretroviral compounds during gestation and nursing

Abstract Objective There is evidence of ototoxicity from antiretrovirals (ARVs), and ARV therapy in pregnant/nursing mothers can expose offspring to these compounds. The current work modelled whether exposure to ARVs in utero and during nursing altered the functioning of the auditory system in offspring mice. Design The females of seven breeding pairs of C57BL6/J mice were given daily doses of ARVs lamivudine and tenofovir disoproxil fumarate by oral gavage during gestation and nursing. Three breeder females were given equivalent volumes of water as controls. At wean age (3 weeks after birth), the offspring mice were tested with auditory brainstem responses (ABRs). At the conclusion of the experiment, the offspring mice’s cochleae were examined for hair cell counts. Study sample Ten breeder female C57BL6/J mice and 69 offspring mice. Results The offspring mice exposed to ARVs during development showed higher ABR thresholds than the control offspring. No differences were found in supra-threshold ABRs. There was no evidence of missing hair cells. Conclusions Hearing impairment may be a possible consequence of exposure to ARVs during gestation and development. Because the threshold differences were not large, if they are occurring in humans, it is unlikely they would be identified in any hearing screening tests.
A Nonlinear Model Predictive Control Algorithm for an Unmanned Ground Vehicle on Variable Terrain

This thesis presents a Nonlinear Model Predictive Controller (NMPC) for an Unmanned Ground Vehicle (UGV) capable of controlling the vehicle over both smooth and rough terrain using measurements from GPS and a Light Detection And Ranging (LiDAR) unit equipped to the vehicle. Linear Model Predictive Control (MPC) and NMPC have become more widely used to control dynamic systems as computers have become more capable of handling the computational expense required by model predictive control. Though the use of NMPC rather than linear MPC creates an additional computational expense, NMPC allows for path planning in addition to control of the vehicle. This is particularly advantageous in scenarios in which the UGV is traversing terrain that contains obstacles of which the vehicle has no a priori knowledge. Rough, off-road terrain contains multiple hazards for an UGV. In this thesis, hazards are classified into three groups: obstacles, rough traversable terrain, and rough untraversable terrain. These three types of hazards create a rollover risk for a UGV. The NMPC presented in this thesis is designed to mitigate this risk of rollover. Simulations of the NMPC in several different scenarios are presented, as well as results from experimental implementation of the NMPC on a test vehicle. Results from simulation and experimental implementation are provided that show the NMPC is able to navigate a UGV around obstacles to a target location without requiring the use of a priori knowledge of terrain and obstacles.
Expository discourse skills of students with reading comprehension difficulties

Abstract Purpose: The ability to produce expository discourse (the use of language to convey information) is important for classroom participation and access to the curriculum, particularly during the middle school years. This study investigated the spoken expository discourse skills of students with reading comprehension (RC) difficulties compared to their peers with average reading skills. Method: In this study, we administered a modified favourite game or sport (M-FGS) task developed by Heilmann and Malone to 48 students who were in their fifth year of schooling (9.33 − 11.11 years of age). Expository language samples were transcribed and analysed on measures of (a) microstructure: syntax (MLU in words) and vocabulary (number of different words [NDW]); and (b) macrostructure (Expository Scoring Scheme [ESS]). Result: Compared to their peers with average RC skills, students with RC difficulties demonstrated significant difficulties at the micro- and macro-structure levels. Subgroup analysis revealed the importance of spoken language comprehension proficiency (at text level) for expository discourse skills. Conclusion: The results from this small-scale investigation demonstrated the usefulness of the M-FGS task in describing challenges in expository discourse of students with RC difficulties, with clear implications for intervention.
Dementia-friendly initiatives within the context of COVID-19 pandemic: Challenges and strategies perceived by service professional stakeholders from the USA and China

Purposes Dementia-friendly initiatives (DFI) are community-based movements aimed to address stigma, exclusion, and discrimination associated with dementia. This study examined the challenges faced and strategies used by DFI prior to and during the COVID-19 pandemic from the perspectives of stakeholders in the USA and China. Methods Qualitative interviews with 17 stakeholders involved in DFI from the United States and mainland China were conducted via the Zoom platform. Semi-structured interview questions focused on DFI challenges and strategies prior to and during the pandemic. Thematic analysis was used to analyze the data. Results Three major challenges prior to the COVID-19 pandemic included low participation of persons with dementia, difficulties in building community collaborations, and limited funding and resources needed to sustain DFI. During the COVID-19 pandemic, challenges included exacerbated difficulties of involving persons with dementia and reduced policy support for DFI. Strategies implemented prior to COVID-19 included partnerships with community organizations to outreach and engage persons with dementia, and coordination of resources and diversification of funding sources to sustain DFI. Strategies during the COVID-19 pandemic centered on the implementation of person-centered technology to support persons with dementia and family caregivers, and the development of new programs that integrated efforts to address the impact of COVID-19. Implications DFI in the USA and mainland China shared similar challenges for DFI prior to and during COVID-19. During the COVID-19 pandemic, DFI in both countries showed resourcefulness through reliance on technology, community collaboration, and COVID-19–related resources to provide support and services. While it remains critical to advocate to the central government to fund DFI, DFI in both societies need to be open to other funding sources, hire persons with dementia as key staff members of DFI, and demonstrate its effectiveness through rigorous evaluation.
Temperature and Rainfall Patterns Constrain the Multidimensional Rewilding of Global Forests

The long‐term contribution of global forest restoration to support multiple dimensions of biodiversity and ecosystem function remains largely illusive across contrasting climates and forest types. This hampers the capacity to predict the future of forest rewilding under changing global climates. Here, 120 studies are synthesized across five continents, and it is found that forest restoration promotes multiple dimensions of biodiversity and ecosystem function such as soil fertility, plant biomass, microbial habitat, and carbon sequestration across contrasting climates and forest types. Based on global relationship between stand age and soil organic carbon stock, planting 350 million hectares of forest under the UN Bonn Challenge can sequester >30 Gt soil C in the surface 20 cm over the next century. However, these findings also indicate that predicted increases in temperature and reductions in precipitation can constrain the positive effects of forest rewilding on biodiversity and ecosystem function. Further, important tradeoffs are found in very old forests, with considerable disconnection between biodiversity and ecosystem function. Together, these findings provide evidence of the importance of the multidimensional rewilding of forests, suggesting that on‐going climatic changes may dampen the expectations of the positive effects of forest restoration on biodiversity and ecosystem function.
International Validation of the Canadian Syncope Risk Score

BACKGROUND The Canadian Syncope Risk Score (CSRS) was developed to predict 30-day serious outcomes not evident during emergency department (ED) evaluation. OBJECTIVE To externally validate the CSRS and compare it with another validated score, the Osservatorio Epidemiologico della Sincope nel Lazio (OESIL) score. DESIGN Prospective cohort study. SETTING Large, international, multicenter study recruiting patients in EDs in 8 countries on 3 continents. PARTICIPANTS Patients with syncope aged 40 years or older presenting to the ED within 12 hours of syncope. MEASUREMENTS Composite outcome of serious clinical plus procedural events (primary outcome) and the primary composite outcome excluding procedural interventions (secondary outcome). RESULTS Among 2283 patients with a mean age of 68 years, the primary composite outcome occurred in 7.2%, and the composite outcome excluding procedural interventions occurred in 3.1% at 30 days. Prognostic performance of the CSRS was good for both 30-day composite outcomes and better compared with the OESIL score (area under the receiver-operating characteristic curve [AUC], 0.85 [95% CI, 0.83 to 0.88] vs. 0.74 [CI, 0.71 to 0.78] and 0.80 [CI, 0.75 to 0.84] vs. 0.69 [CI, 0.64 to 0.75], respectively). Safety of triage, as measured by the frequency of the primary composite outcome in the low-risk group, was higher using the CSRS (19 of 1388 [0.6%]) versus the OESIL score (17 of 1104 [1.5%]). A simplified model including only the clinician classification of syncope (cardiac syncope, vasovagal syncope, or other) variable at ED discharge-a component of the CSRS-achieved similar discrimination as the CSRS (AUC, 0.83 [CI, 0.80 to 0.87] for the primary composite outcome). LIMITATION Unable to disentangle the influence of other CSRS components on clinician classification of syncope at ED discharge. CONCLUSION This international external validation of the CSRS showed good performance in identifying patients at low risk for serious outcomes outside of Canada and superior performance compared with the OESIL score. However, clinician classification of syncope at ED discharge seems to explain much of the performance of the CSRS in this study. The clinical utility of the CSRS remains uncertain. PRIMARY FUNDING SOURCE Swiss National Science Foundation & Swiss Heart Foundation.
“They have given you the morale and confidence:” adolescents and young adults want more community-based oral HIV self-testing options in Kenya

ABSTRACT Community-based delivery of oral HIV self-testing (HIVST) may expand access to testing among adolescents and young adults (AYA). Eliciting youth perspectives can help to optimize these services. We conducted nine focus group discussions (FGDs) with HIV negative AYA aged 15–24 who had completed oral HIVST following community-based distribution through homes, pharmacies, and bars. FGDs were stratified by distribution point and age (15–17, 18–24). Participants valued HIVST because it promoted greater autonomy and convenience compared to traditional clinic-based testing. AYA noted how HIVST could encourage positive behavior change, including using condoms to remain HIV negative. Participants recommended that future testing strategies include individualized, ongoing support during and after testing. Support examples included access to trained peer educators, multiple community-based distribution points, and post-test support via phones and websites. Multiple distribution points and trained peer educators’ involvement in all steps of distribution, testing, and follow-up can enhance future community-based HIVST programs.
The effect of rapid weight loss on sports injury in elite taekwondo athletes

ABSTRACT Objectives The purpose of this study was to reveal the relationship of injury patterns between normal training period and weight loss period in Korea elite taekwondo athletes. Methods Since 2021, data for elite taekwondo athletes have been collected prospectively by the Korean Training Institute, there were 102 male and 95 female. The data were classified by sex, weight class, injury location, injuries during the normal training and weight loss periods. The χ2 test was used to compare groups. The injury incidence rates were calculated as the number of injuries per 1,000 hours of training. Results A total of 703 injuries were recorded during normal training, and total of 149 injuries were recorded during the weight loss period. Taekwondo athletes showed a higher incidence of during the weight loss period than during the normal training period (3.66 VS 6.88; p < 0.001). The body parts affected by injury differed significantly between the normal training period and weight loss period (p = 0.033). Injury to the lower extremities or to the head and neck was relatively higher during the normal training period, while injury to the trunk and upper extremities was relatively higher during the weight loss period. Muscle, ligament, and bone injuries were common during both the normal training and weight loss periods. Level I injuries were relatively more frequent during normal training, while level II and III injuries were relatively more frequent during weight loss (p < 0.001). Conclusion Rapid weight loss is related to the injury patterns in taekwondo athletes. Injury incidence rate increases during rapid weight loss periods. Moreover, the injury site and injury severity depend on during normal training and during the weight loss period.
The role of the genetic variant FECH rs11660001 in the occurrence of anti‐tuberculosis drug‐induced liver injury

The pathogenic mechanism of anti‐tuberculosis drug‐induced liver injury (AT‐DILI) is still largely unknown. Recent studies have indicated that rifampicin and isoniazid cotreatment causes the accumulation of endogenous protoporphyrin IX in the liver through the haem biosynthesis pathway. Alanine synthase 1 (ALAS1) and ferrochelatase (FECH) are the rate‐limiting enzymes in the production of haem. The present study aimed to investigate the genetic contribution of the ALAS1 and FECH genes to the risk of AT‐DILI in an Eastern Chinese Han population.
The role of the unilateral pectoralis major muscle flap in the treatment of deep sternal wound infection and dehiscence

Deep sternal wound infection and dehiscence has been classified as complex wound, and its treatment is a challenge for the surgeon. There are many flap choices for its treatment, each one having advantages and drawbacks. The article by Wang et al. evidenced that the unilateral pectoralis major muscle flap is a simple and effective option for wound closure resulting from sternotomy dehiscence in infants and children. The report discussed herein highlights that the unilateral pectoralis major muscle flap has been a good and feasible option for the reconstruction of the sternal wound in adults, as previously described by our group and other authors. This technique presents low morbidity and acceptable esthetic and functional results, providing stability to the sternal region.
The Nab2 RNA-binding protein patterns dendritic and axonal projections through a planar cell polarity-sensitive mechanism

Abstract RNA-binding proteins support neurodevelopment by modulating numerous steps in post-transcriptional regulation, including splicing, export, translation, and turnover of mRNAs that can traffic into axons and dendrites. One such RNA-binding protein is ZC3H14, which is lost in an inherited intellectual disability. The Drosophila melanogaster ZC3H14 ortholog, Nab2, localizes to neuronal nuclei and cytoplasmic ribonucleoprotein granules and is required for olfactory memory and proper axon projection into brain mushroom bodies. Nab2 can act as a translational repressor in conjunction with the Fragile-X mental retardation protein homolog Fmr1 and shares target RNAs with the Fmr1-interacting RNA-binding protein Ataxin-2. However, neuronal signaling pathways regulated by Nab2 and their potential roles outside of mushroom body axons remain undefined. Here, we present an analysis of a brain proteomic dataset that indicates that multiple planar cell polarity proteins are affected by Nab2 loss, and couple this with genetic data that demonstrate that Nab2 has a previously unappreciated role in restricting the growth and branching of dendrites that elaborate from larval body-wall sensory neurons. Further analysis confirms that Nab2 loss sensitizes sensory dendrites to the genetic dose of planar cell polarity components and that Nab2-planar cell polarity genetic interactions are also observed during Nab2-dependent control of axon projection in the central nervous system mushroom bodies. Collectively, these data identify the conserved Nab2 RNA-binding protein as a likely component of post-transcriptional mechanisms that limit dendrite growth and branching in Drosophila sensory neurons and genetically link this role to the planar cell polarity pathway. Given that mammalian ZC3H14 localizes to dendritic spines and controls spine density in hippocampal neurons, these Nab2-planar cell polarity genetic data may highlight a conserved path through which Nab2/ZC3H14 loss affects morphogenesis of both axons and dendrites in diverse species.
Manipulation and Mechanical Deformation of Leukemia Cells by High-Frequency Ultrasound Single Beam

Ultrasound single-beam acoustic tweezer system has attracted increasing attention in the field of biomechanics. Cell biomechanics play a pivotal role in leukemia cell functions. To better understand and compare the cell mechanics of the leukemia cells, herein, we fabricated an acoustic tweezer system in-house connected with a 50-MHz high-frequency cylinder ultrasound transducer. Selected leukemia cells (Jurkat, K562, and MV-411 cells) were cultured, trapped, and manipulated by high-frequency ultrasound single beam, which was transmitted from the ultrasound transducer without contacting any cells. The relative deformability of each leukemia cell was measured, characterized, and compared, and the leukemia cell (Jurkat cell) gaining the highest deformability was highlighted. Our results demonstrate that the high-frequency ultrasound single beam can be utilized to manipulate and characterize leukemia cells, which can be applied to study potential mechanisms in the immune system and cell biomechanics in other cell types.
Choosing By Advantages incorporated framework for a user-involved design process

ABSTRACT Participation of stakeholders, such as users, in the design process remains significant in generating value of construction projects. This calls for new approaches in the interactions between designers and users towards collaboratively understanding and meeting user needs. A systematic framework, incorporating the Choosing By Advantages (CBA) decision system is, therefore, proposed as a contribution to improving designer–user collaboration for value generation in construction projects. The framework, resulting from an action-oriented constructive research approach, is based on a combination of empirical knowledge from the case application of CBA on selected projects in Ghana, and relevant theory. It is structured along the Input-Mediator-Output-Input scheme of team process, containing six performance episodes connected with reciprocal dependency loops. Guided by the pragmatist stance on truth, the workability of the framework was evaluated in a case study. The paper contributes to design process management and stakeholder involvement by providing a systematic guide on employing a multi-attribute decision system for designers and users to collaboratively discover, generate and authenticate value at the design stage of construction projects.
Hippocampals neurogenesis is impaired in mice with a deletion in the coiled coil domain of Talpid3—implications for Joubert syndrome

Abstract Mutations in Talpid3, a basal body protein essential for the assembly of primary cilia, have been reported to be causative for Joubert Syndrome (JS). Herein, we report prominent developmental defects in the hippocampus of a conditional knockout mouse lacking the conserved exons 11 and 12 of Talpid3. At early postnatal stages, the Talpid3 mutants exhibit a reduction in proliferation in the dentate gyrus and a disrupted glial scaffold. The occurrence of mis-localized progenitors in the granule cell layer suggests a role for the disrupted glial scaffold in cell migration resulting in defective subpial neurogenic zone-to-hilar transition. Neurospheres derived from the hippocampus of Talpid3fl/flUbcCre mouse, in which Talpid3 was conditionally deleted, lacked primary cilia and were smaller in size. In addition, neurosphere cells showed a disrupted actin cytoskeleton and defective migration. Our findings suggest a link between the hippocampal defects and the learning/memory deficits seen in JS patients.
Platelet Function Is Associated With Dementia Risk in the Framingham Heart Study

Background Vascular function is compromised in Alzheimer disease (AD) years before amyloid and tau pathology are detected and a substantial body of work shows abnormal platelet activation states in patients with AD. The aim of our study was to investigate whether platelet function in middle age is independently associated with future risk of AD. Methods and Results We examined associations of baseline platelet function with incident dementia risk in the community‐based FHS (Framingham Heart Study) longitudinal cohorts. The association between platelet function and risk of dementia was evaluated using the cumulative incidence function and inverse probability weighted Cox proportional cause‐specific hazards regression models, with adjustment for demographic and clinical covariates. Platelet aggregation response was measured by light transmission aggregometry. The final study sample included 1847 FHS participants (average age, 53.0 years; 57.5% women). During follow‐up (median, 20.5 years), we observed 154 cases of incident dementia, of which 121 were AD cases. Results from weighted models indicated that platelet aggregation response to adenosine diphosphate 1.0 µmol/L was independently and positively associated with dementia risk, and it was preceded in importance only by age and hypertension. Sensitivity analyses showed associations with the same directionality for participants defined as adenosine diphosphate hyper‐responders, as well as the platelet response to 0.1 µmol/L epinephrine. Conclusions Our study shows individuals free of antiplatelet therapy with a higher platelet response are at higher risk of dementia in late life during a 20‐year follow‐up, reinforcing the role of platelet function in AD risk. This suggests that platelet phenotypes may be associated with the rate of dementia and potentially have prognostic value.