Miracle-dz/UniFAR-FTUs huggingface dataset:
Blends of gelatin and hyaluronic acid stratified by stereolithographic bioprinting approximate cartilaginous matrix gradients.

Stereolithographic bioprinting holds great promise in the quest for creating artificial, biomimetic cartilage-like tissue. To introduce a more biomimetic approach, we examined blending and stratifying methacrylated hyaluronic acid (HAMA) and methacrylated gelatin (GelMA) bioinks to mimic the zonal structure of articular cartilage. Bioinks were suspended with porcine chondrocytes before being printed in a digital light processing approach. Homogenous constructs made from hybrid bioinks of varying polymer ratios as well as stratified constructs combining different bioink blends were cultivated over 14 days and analyzed by histochemical staining for proteoglycans/collagen type II, cartilage marker expression analysis, and for cellular viability. The stiffness of blended bioinks increased gradually with HAMA content, from 2.41 ± 0.58 kPa (5% GelMA, 0% HAMA) to 8.84 ± 0.11 kPa (0% GelMA, 2% HAMA). Cell-laden constructs maintained vital chondrocytes and supported the formation of proteoglycans and collagen type II. Higher concentrations of GelMA resulted in increased formation of cartilaginous matrix proteins and a more premature phenotype. However, decreased matrix production in central areas of constructs was observed in higher GelMA content constructs. Biomimetically stratified constructs retained their gradient-like structure even after ECM formation, and exclusively exhibited a significant increase in COL2A1 gene expression (+178%). Concluding, we showed the feasibility of blending and stratifying photopolymerizable, natural biopolymers by SLA bioprinting to modulate chondrocyte attributes and to create zonally segmented ECM structures, contributing to improved modeling of cartilaginous tissue for regenerative therapies or in vitro models.
The effect of backpack weight on the performance of basic short-term/working memory tasks while walking along a pre-determined route

Abstract This study investigated possible backpack weight effects on the performance of three basic short-term/working memory (STM/WM) tasks conducted concurrently with the physical task of route walking. The STM/WM tasks were the Corsi block-tapping, digit span, and 3-back tasks, and, were employed to examine the visuo-spatial sketchpad, phonological loop and central executive components of the WM system. Four backpack weight levels (0%, 15%, 25% and 40% of body mass) were considered. Thirty participants conducted the three experimental tasks requiring physical-cognitive multitasking. Data analyses revealed that: (1) increased backpack weight resulted in decreases in the performance of the Corsi block-tapping and the 3-back task, but (2) backpack weight did not significantly affect the digit span task performance. The study results suggest that reducing backpack weight could benefit the performance of various cognitive tasks during route walking. The study findings may be useful for the ergonomics design of body-worn equipment and human-system interfaces. Practitioner summary: This study examined the backpack weight effects on the performance of three basic short-term/working memory tasks conducted concurrently with the physical task of route walking. The study revealed that reducing backpack weight could benefit various cognitive tasks during physical-cognitive multitasking, especially cognitive tasks that require visuospatial processing and executive control.
Design of Multi-Channel Photoelectric Signals Acquisition and Storage System Based on FPGA

In the view of the problem on the measurement of projectile coordinate based on laser screen thatneeds massive optical signals, the design of massive optical signals acquisition and storage system is proposed. FP-GA is used as the chip of acquisition and control and FLASH is used as the chip of storage. The collected photoelec-tric signals are temporarily placed in the FPGA build-in FIFO, and then these signals are processed in the FPGAand stored in the FLASH, and USB interface is used to communicate with computer. The simulation results showthat the system could collect and store 64 digital signals successfully, it has flexible structure, simple operation, ac-curate data and large storage.
Current state of preconception care in sub-Saharan Africa: A systematic scoping review

Background Preconception care (PCC) utilisation is essential to extend and complete the health continuum. However, these services are not yet incorporated into many low-income countries’ existing maternal health services. Aim This study aims to review the current literature on the knowledge, utilisation and provision of PCC. Setting This included women and healthcare workers (HCWs) in Sub-Saharan African (SSA) countries. Methods Arksey and O’Malley’s scoping review methodology framework is used in this study. The following databases, Google Scholar, Science Direct, PubMed, Scopus and Dissertation via ProQuest, were searched. Articles that met the eligibility criteria were included in this study. Results Out of the 451 retrieved articles, 39 were relevant. In most studies, women’s utilisation and HCW’s provision of PCC were considered limited. Their knowledge, however, varies between studies, and there were a few studies conducted among women with chronic conditions. Several factors influenced women and HCWs’ knowledge, utilisation and provision of PCC, including age, level of education, employment, practice area, resources and knowledge. Preconception care interventions most commonly identified, utilised and provided were HIV testing, counselling and family planning, while preconception folic acid supplementation was the least. Conclusion The estimates of knowledge and utilisation were suboptimal among women, while provision was the worst affected among HCWs. Gaps exist between the HCW knowledge and practice of PCC. There is a need to promote, prioritise, integrate and optimise the opportunistic provision of PCC in SSA. There is also a need for more studies on PCC provision and utilisation among women with chronic medical conditions.
Angiotensin1-7 Protects Against Renal Ischemia-Reperfusion Injury via Regulating the Expression of NRF2 and microRNAs in Fisher 344 Rats.

Ischemia/reperfusion (I/R) is considered the primary cause of acute kidney injury and is higher among older individuals. While ischemic episodes are hard to predict and prevent, detrimental ischemic effects could be mitigated by exogenous intervention. This study aims to identify the protective role of angiotensin (ANG)1-7 against I/R-induced renal injury in adult vs. aged rats. Adult and aged male Fisher 344 rats were subjected to 40-minute bilateral renal ischemia followed by 28-days reperfusion. ANG1-7 was administered intraperitoneally in ischemic rats for 28 days without or with Mas receptor antagonist A779. I/R increased blood pressure, plasma creatinine, urinary 8-isoprostane, and renal infiltration of pro and anti-inflammatory macrophages and reduced glomerular filtration rate in both adult and aged rats compared to shams. In addition to causing glomerular sclerosis and tubular damage, I/R increased the expression of pathogenic microRNAs (miRNAs): miR-20a-5p, miR-21-5p, miR-24-3p, and miR-194-5p in both the age groups. ANG1-7 treatment of ischemic rats mitigated oxidative stress and renal inflammation, restored renal structure and function, and reduced high blood pressure. Also, ANG1-7 suppressed the expression of pathogenic miRNAs. In addition, ANG1-7 treatment of I/R rats increased the expression of redox-sensitive transcription factor NRF2 and phase II antioxidant enzymes. The beneficial effects of ANG1-7 were sensitive to A779. Collectively, these data suggest that ANG1-7 associated with NRF2 activation could alleviate post-I/R-induced kidney injury and therefore serve as a potential therapeutic compound to protect against biochemical and morphological pathologies of I/R in both adults and aged populations.
Reappraisal of sepsis-3 and CLIF-SOFA as predictors of mortality in patients with cirrhosis and infection presenting to the emergency department: A multicenter study

Background/Aims Sepsis-3 criteria and quick Sequential Organ Failure Assessment (qSOFA) have been advocated to be used in defining sepsis in the general population. We aimed to compare the Sepsis-3 criteria and Chronic Liver Failure-SOFA (CLIF-SOFA) scores as predictors of in-hospital mortality in cirrhotic patients admitted to the emergency department (ED) for infections. Methods A total of 1,622 cirrhosis patients admitted at the ED for infections were assessed retrospectively. We analyzed their demographic, laboratory, and microbiological data upon diagnosis of the infection. The primary endpoint was inhospital mortality rate. The predictive performances of baseline CLIF-SOFA, Sepsis-3, and qSOFA scores for in-hospital mortality were evaluated. Results The CLIF-SOFA score proved to be significantly better in predicting in-hospital mortality (area under the receiver operating characteristic curve [AUROC], 0.80; 95% confidence interval [CI], 0.78–0.82) than the Sepsis-3 (AUROC, 0.75; 95% CI, 0.72–0.77, P<0.001) and qSOFA (AUROC, 0.67; 95% CI, 0.64–0.70; P<0.001) score. The CLIF-SOFA, CLIF-C-AD scores, Sepsis-3 criteria, septic shock, and qSOFA positivity were significantly associated with in-hospital mortality (adjusted hazard ratio [aHR], 1.24; 95% CI, 1.19–1.28; aHR, 1.13; 95% CI, 1.09–1.17; aHR, 1.19; 95% CI, 1.15–1.24; aHR, 1.88; 95% CI, 1.42–2.48; aHR, 2.06; 95% CI, 1.55–2.72; respectively; all P<0.001). For CLIF-SOFA scores ≥6, in-hospital mortality was >10%; this is the cutoff point for the definition of sepsis. Conclusions Among cirrhosis patients presenting with infections at the ED, CLIF-SOFA scores showed a better predictive performance for mortality than both Sepsis-3 criteria and qSOFA scores, and can be a useful tool of risk stratification in cirrhotic patients requiring timely intervention for infection.
Functional Assessment of 3D-Printed Multifunction Assistive Hand Device for Chronic Stroke Patients

Patients with chronic stroke often have difficulty opening their hands and performing grasping movements. Several passive hand orthoses for assisting hand rehabilitation have been developed and demonstrated to be clinically effective. However, current devices have several limitations, such as supporting only a single grasping motion and using an abnormal grasping posture. Therefore, this study developed a three-dimensional (3D)-printed multifunctional hand device (3DP-MFHD) to solve these problems and evaluated the feasibility of using the device during home rehabilitation. Six participants were enrolled, and each of them was provided with the 3DP-MFHD. In addition to a task-oriented training course, the participants were asked to train at home for 4 weeks for at least 5 days per week and 40 min per day. The results revealed that hand grip force increased by 36.1%, lateral pinch force increased by 17.6%, and the Action Research Arm Test score increased by 54.1%. The 3DP-MFHD is a promising means to facilitate hand rehabilitation and improve hand strength and function in patients with chronic stroke. The 3DP-MFHD can be used as part of home rehabilitation.
Use of the von Willebrand factor concentrate with low factor VIII content to manage patients with inherited von Willebrand disease requiring surgical or secondary long‐term prophylaxis: An expert opinion paper from an Italian panel

The present review aims to summarize the state‐of‐the‐art von Willebrand disease (VWD) treatment focusing on specific clinical settings (obstetrics, surgery, long‐term prophylaxis and comorbidities) as well as on the use of a Von Willebrand factor (VWF) concentrate with low FVIII content.
Halo‐seq: An RNA Proximity Labeling Method for the Isolation and Analysis of Subcellular RNA Populations

The subcellular localization of specific RNA molecules promotes localized cellular activity across a variety of species and cell types. The misregulation of this RNA targeting can result in developmental defects, and mutations in proteins that regulate this process are associated with multiple diseases. For the vast majority of localized RNAs, however, the mechanisms that underlie their subcellular targeting are unknown, partly due to the difficulty associated with profiling and quantifying subcellular RNA populations. To address this challenge, we developed Halo‐seq, a proximity labeling technique that can label and profile local RNA content at virtually any subcellular location. Halo‐seq relies on a HaloTag fusion protein localized to a subcellular space of interest. Through the use of a radical‐producing Halo ligand, RNAs that are near the HaloTag fusion are specifically labeled with spatial and temporal control. Labeled RNA is then specifically biotinylated in vitro via a click reaction, facilitating its purification from a bulk RNA sample using streptavidin beads. The content of the biotinylated RNA is then profiled using high‐throughput sequencing. In this article, we describe the experimental and computational procedures for Halo‐seq, including important benchmark and quality control steps. By allowing the flexible profiling of a variety of subcellular RNA populations, we envision Halo‐seq facilitating the discovery and further study of RNA localization regulatory mechanisms. © 2022 Wiley Periodicals LLC.
Phenotypic expansion and variable expressivity in individuals with JARID2‐related intellectual disability: A case series

Loss of function variants in JARID2 were recently reported in 16 patients with a neurodevelopmental disorder characterized by delays, intellectual and learning disability, autism, behavioral abnormalities, and dysmorphic features. Most cases were de novo, with only one variant inherited from an affected parent. Here, we present seven additional individuals from five families with pathogenic or likely pathogenic JARID2 variants, confirming this gene‐disease association and highlighting palatal abnormalities and heart defects as part of the phenotype. In addition, we report inheritance of JARID2 variants from mildly affected parents, demonstrating the variable expressivity of the disease. We also note the high prevalence of intragenic JARID2 copy number variants, emphasizing the importance of exon‐level analysis.
Coping with ill-health while lacking access to health care: Acceptability of health service provision in rural Malawi – a qualitative study

ABSTRACT Background Large parts of Malawi`s population lack access to health care. A high burden of disease, chronic poverty, and a growing population accelerate the need for extending and improving health care. One region that is struggling with service provision is Malawi´s rural district Phalombe. In addition to adequate resources, acceptability of service provision and productive patient-provider engagements are crucial determinants of health-seeking behaviour. Objective This study aimed to better understand the interdependencies between acceptability, patient-provider engagement, and health-seeking behaviour in Phalombe. By targeting health care providers and community members, different perspectives were assessed and triangulated. Methods Following a qualitative approach, group interviews were conducted with community members of three rural villages (n = 21) in Phalombe. Semi-structured interviews (n = 2) and a group interview among management staff (n = 3) provided insight into experiences of health care providers. Results Community members perceived health care providers’ behaviour as disrespectful, resulting in power gaps between patients and providers. Providers blamed community members’ cultural beliefs and lack of awareness regarding health care as barriers to seek formal services. Systemic shortcomings diminished community members’ trust in service provision, while increasing frustration among providers and thus impacting patient-provider engagement. Due to insufficient resources, lack of acceptability and trust in receiving adequate services, potential patients turned into non-users of health care. Conclusions A patient-centred approach is needed that empowers communities by involving them in health care planning, in facility management, and by raising awareness towards health issues. Trainings for providers need to focus on improving communication and building trustful patient-provider interactions. Yet, without addressing systemic constraints, providers’ frustration and patients’ lack of trust in service provision will remain and impact their health-seeking behaviour. Thus, further budget needs to be allocated to Malawi’s health care sector in order to provide resources needed.
The Use of Graphene and its Derivatives in Chemical and Biological Sensing

Abstract A chemical sensor is defined as a transducer comprised of, or coated with, a layer that responds to changes in its local chemical environment. Chemical sensors convert various forms of energy into a measurable signal. For instance, the chemical energy involved with bonds breaking or forming can change the electronic properties of the transducer, creating an observable signal such as an increase or decrease in electrical resistance. Chemical sensing is important in many facets of research including environmental, bio-medical/pharmaceutical, industrial, automotive, and human safety. For a sensor to be practical it must interact preferentially with the target chemical analyte. A sensor should be precise, accurate, robust, cost efficient to manufacture, low in power consumption, portable otherwise the sensor is undesirable. Another key value of chemical sensors is it must exhibit rapid detection. Prior to portable sensors chemical analysis was performed in a laboratory on large, expensive instruments, which is costly in time, equipment fees, and personnel wages to operate. These sophisticated instruments are accurate and precise, however, it is far more beneficial to have a miniature, on-site detection apparatus. The first environmental, on-site sensor was used by the mining industry to monitor subterranean air quality; the canary. Carbon monoxide and methane (colorless, odorless gases) are large iv problems in the mining industry; smaller life forms are more susceptible to being poisoned by toxic gases. Today sensor constructs are far different from that of a canary, however, they serve the same purpose. Carbon nanomaterials such as graphene and single-walled carbon nanotubes and other derivatives prove to be of great importance in sensor research due to their unique electronic properties, and they’re high aspect ratio allowing them to be highly sensitive to small perturbations in local electronic environments.
Electrical erosion test and condition assessment of SF6 CB contact sets

Dynamic resistance measurement (DRM) was proved to be an important method for condition assessment of contact sets of SF6 circuit breakers (CBs). The erosion of contact sets due to arc energy is a main factor that influences the electrical lifetime of the extinguishing chamber. In this study, a high-voltage CB synthetic circuit was used to produce fault current for electrical erosion tests on the contact sets. The contact sets were close to the end of their service life after a series of erosion tests. DRMs with DC amperages of 0.5, 1.0, and 1.5 kA were conducted on the two tested breakers in the interval of each erosion test. Five essential diagnostic parameters were extracted from the contact resistance curves as a function of contact travel. The variation of these parameters during the whole erosion tests were investigated to find an effective condition assessment method for contact sets. From the power balance at the contact surface, the material erosion rate due to metal evaporation for each erosion test was calculated and in good agreement to the experimental results. Finally, the contact length and contact resistance were employed as diagnostic parameters for condition assessment of contact sets.
Nursing training and retraining on cardiopulmonary resuscitation: a theoretical-practical intervention

ABSTRACT Objective: To analyze the retention of knowledge and skills of nursing professionals following training and retraining on cardiopulmonary resuscitation. Method: This is an intervention, prospective, and analytical study in which 56 nursing professionals received theoretical and practical training in in-service cardiopulmonary resuscitation. Nine months after the first training (T1), these professionals participated in a retraining (T2). They were followed up for 18 months. The linear trend of knowledge and skills in the period following training was calculated and the Wilcoxon test was applied. Results: Interventions increased the knowledge and skills of professionals significantly; however, in the subsequent period, skills decreased. Despite this, after a period of nine months, they were still higher than those identified before the study. There was a reduction of 18.2% in knowledge in the theoretical test after T1 vs 13.0% after T2 (p < 0.01) and a reduction of 7.6% in skills on the practical test after T1 vs 5.3% after T2 (p < 0.01). Conclusion: Nurses were able to retain more knowledge and skills on cardiopulmonary resuscitation after retraining, which stresses the importance of regular training and continuing education in health.
Sixth Grade Students’ Images Of Engineering: What Do Engineers Do?

There has been a growing interest in teaching engineering in the K-12 context. It is therefore somewhat surprising to find that there have been few investigations on students’ views of engineering and the nature of engineering (VNOE) at this level. This project is based on the assumption that understanding students’ VNOE has as much potential for impact on engineering education as the views of the nature of science investigations have had on the field of science education. The overall goal of this study was to investigate 6 th -grade students’ perceptions of the nature of engineering (NOE), and to understand the experiences that have shaped students’ perceptions of the field of engineering. The results of twenty 6 th grade students’ drawings of an engineer or engineers at work with associated interviews related students’ own drawings from two mid-west middle schools are presented. Results indicated many students held common misand naïve conceptions about engineering, engineers, and what engineers do; specifically that engineers drive trains, fix cars, build houses, and work alone.
Research of AP1000 passive core cooling system and system design transient

The loss of active core cooling system of the plant immediately relates to severe safety accident.A unique safety-related system,called the passive core cooling system(PXS),is applied in AP1000 standard design,since a special steel containment shell is adopted.The PXS utilizes passive means to achieve the reactor safety functions: core cooling,coolant make-up,and radioactivity release limiting.This report emphasizes on system function design,and then discusses system design transients by using the bounding method that focuses on some important design transients.
Potensi Waste to Energy Sampah Perkotaan untuk Kapasitas Pembangkit 1 MW di Propinsi DIY

The aim of this work was to study the potential of the waste to be converted into electrical energy (waste to energy) with a target capacity of the biomass power plant (PLTBiomassa) of 1MW. This study was focused in DIY province-Indonesia and the organic waste type used is municipal solid waste (MSW) that collected at the landfill area. The method used i n this paper is by calculat ing the number of waste based on the composition of waste and finding the potential of electric al power that can be converted from each type of waste through thermochemical and biochemical conversion process. The amount of waste collected in the landfill in DIY province have been sufficient to fulfill target of 1 MW electrical power. The potential of power generated by MSW in landfill are 1541.97 kW generator with 1039.95 kW for thermochemical system (gasification technology) and 502.02 kW for biochemcal system (anaerobic digestion).