Miracle-dz/UniFAR-FTUs huggingface dataset:
Profit Maximization Using Knapsack Algorithm for Resource Allocation in Cloud

: Cloud computing allows the customers to dynamically change their requirements on the pay-as-you-use manner. The SLA is an agreement normally negotiated and accepted between the service provider and user. The provisioning of resources can be either lower bound or upper bound, which results in the violation of SLA or wastage of resources and money respectively. In order to overcome such defects an algorithm is used to calculate the number of virtual mahines to satisfy all tenants service level agreement based on which resources can be provisioned dynamically. This paper proposes an algorithm that gives results in the maximization of profit to service provider. The integer linear programming and the greedy-heuristic based approaches are used for the provisioning of resources to the clients. The algorithm is generated on the basis of software as a service. In saas perspective, virtual machines are considered as resources. Therefore, the virtual machines are provisioned by the provider to satisfy the dynamic needs of the clients. In some cases, the clients are served earlier for many reasons like they contain short job and minimum deadline. In these cases, some of the virtual machines can be unused, for utilizing the unused virtual machines they are involved in Auction.
RESEARCH PAPER Utilizing hydrogen sulﬁde as a novel anti-cancer agent by targeting cancer glycolysis and pH imbalance

KEY RESULTS Continuous, but not a single, exposure to H2S decreased cell survival more effectively in cancer cells, as compared to non-cancer cells. Slow H2S-releasing donor, GYY4137, significantly increased glycolysis, leading to overproduction of lactate. H2S also decreased anion exchanger and sodium/proton exchanger activity. The combination of increased metabolic acid production and defective pH regulation resulted in an uncontrolled intracellular acidification, leading to cancer cell death. In contrast, no significant intracellular acidification or cell death was observed in non-cancer cells.
Functional Analysis of glioma-associated homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells

The ability to reversibly switch between invasive and non-invasive phenotype has been considered to be critical for the high malignancy of melanoma. Increasing evidence suggests that this ability is determined by the activities of microphthalmia-associated transcription factor (MITF) and EMT-related transcription factors (EMT-TFs). GLI1 and GLI2, the components of Hedgehog (HH) pathway, are associated with progression and invasiveness of melanoma. Although GLI2 is known to suppress MITF expression and to promote invasive phenotype of melanoma, the role of GLI1 remains elusive. Here I show that inhibition of Shh-pathway by cyclopamine or GLI1 knockdown ( Gli1 KD) decreased migration and invasion activity of mouse and human melanoma without affecting MITF levels. I observed that Gli1 KD B16F10 melanoma cells exhibited a loss of mesenchymal-like characteristic as indicated by an increased expression of E-cadherin and a decreased expression of mesenchymal markers. To gain insight into the molecular mechanism, I analyzed several EMT-TFs that are known to regulate melanoma invasiveness. My analysis suggested that the expression of SNAIL1, ZEB1, and TWIST1, but not SNAIL2 and ZEB2, are directly regulated by GLI1. Collectively my findings suggest a regulatory role of GLI1 on a subset of EMT-TFs to promote invasive phenotype of melanoma independent of MITF. An increased expression of Gli1 has been observed in BRAFi-resistant melanoma cells and patient samples, as well as during melanoma progression. Taken together with my present results, GLI1 may play a role in generating a high level of intratumor heterogeneity in melanoma. Targeting GLI1 may therefore be an effective approach for melanoma therapy. Indeed, accumulating evidence suggests that GLI antagonists, of which GANT61 has been most extensively studied in vitro and in animal models, are promising therapeutic candidates for a wide range of cancers, including melanoma.
Tight Coupling of Astrocyte pH Dynamics to Epileptiform Activity Revealed by 3 Genetically-Encoded pH Sensors 4 5 6

58 59 Astrocytes can both sense and shape the evolution of neuronal network activity and are 60 known to possess unique ion regulatory mechanisms. Here we explore the relationship 61 between astrocytic intracellular pH dynamics and the synchronous network activity that 62 occurs during seizure-like activity. By combining confocal and two-photon imaging of 63 genetically-encoded pH reporters with simultaneous electrophysiological recordings, we 64 perform pH measurements in defined cell populations and relate these to ongoing 65 network activity. This approach reveals marked differences in the intracellular pH 66 dynamics between hippocampal astrocytes and neighboring pyramidal neurons in 67 rodent in vitro models of epilepsy. With three different genetically-encoded pH reporters, 68 3 astrocytes are observed to alkalinize during epileptiform activity, whilst neurons are 69 observed to acidify. In addition to the direction of pH change, the kinetics of 70 epileptiform-associated intracellular pH transients are found to differ between the two 71 cell types, with astrocytes displaying significantly more rapid changes in pH. The 72 astrocytic alkalinization is shown to be highly correlated with astrocytic membrane 73 potential changes during seizure-like events and mediated by an electrogenic 74 Na/HCO3 cotransporter. Finally, comparisons across different cell-pair combinations 75 reveal that astrocytic pH dynamics are more closely related to network activity than are 76 neuronal pH dynamics. This work demonstrates that astrocytes exhibit distinct pH 77 dynamics during periods of epileptiform activity, which has relevance to multiple 78 processes including neurometabolic coupling and the control of network excitability. 79
Running head: Rheumatoid arthritis, multimorbidity and health-related outcomes Title: Examining the relationship between rheumatoid arthritis, multimorbidity and adverse health-related outcomes: a systematic review Authors: Jordan Canning1 MRes,

Objective: Multimorbidity (the co-existence of two or more long-term conditions (LTCs)) is highly prevalent in people with rheumatoid arthritis (RA). This work systematically reviewed the literature to determine the effect of multimorbidity on all-cause mortality, functional status and quality of life in RA. Methods: Six electronic databases were searched: CINAHL, Cochrane Library, Embase, Medline, PsycINFO and Scopus. Full-text longitudinal observational studies in English were selected. Quality appraisal of studies was undertaken using the Cochrane-developed QUIPS tool and a narrative synthesis of findings conducted. Results: 5,343 papers were identified by the search strategy, with 19 studies meeting the inclusion criteria. Nine studies had mortality as an outcome, nine reported functional status and/or quality of life and one study reported both mortality and functional status. The number of participants ranged from 183-18,485, with studies conducted between 1985-2018. Mean age of participants ranged from 53.5-66.6 years; with 60.0-84.2% female. Nine studies reported a significant association between multimorbidity and higher risk of mortality in people with RA. Ten studies reported significant associations between multimorbidity and reduced functional status in RA. Three studies also reported a further association with reduced quality of life. Only one study investigated the influence of mental health comorbidities on outcomes. Conclusion: Our review findings indicate that multimorbidity is a significant predictor for higher mortality and poorer functional status/quality of life in people with RA and should be considered in clinical management plans.
A nity maturation of cross-reactive CR3022 antibody against the receptor binding domain of SARS-CoV-2 via in silico site-directed mutagenesis

Samvedna Saini Netaji Subhas University of Technology, New Delhi, India https://orcid.org/0000-0002-3374-2970 Manusmriti Agarwal Netaji Subhas University of Technology, New Delhi, India https://orcid.org/0000-0002-9356-5857 Amartya Pradhan Netaji Subhas University of Technology, New Delhi, India; Tata Institute of Fundamental Research, Mumbai, India https://orcid.org/0000-0001-7575-8404 Savitha Pareek DELL EMC R&D, Bengaluru, India Ashish K Singh DELL EMC R&D, Bengaluru, India Gagan Dhawan Acharya Narendra Dev College, University of Delhi, New Delhi, India https://orcid.org/0000-0001-7492225X Yatender Kumar (  yatender.kumar@nsut.ac.in ) Netaji Subhas University of Technology, New Delhi, India https://orcid.org/0000-0002-9835-9892
Construction and use of a zebrafish heart voltage and calcium optical 1 mapping system, with integrated electrocardiogram and programmable 2 electrical stimulation 3

40 Zebrafish are increasingly being used as a model of vertebrate cardiology due to mammalian- 41 like cardiac properties in many respects. The size and fecundity of zebrafish make them suitable for 42 large-scale genetic and pharmacological screening. In larger mammalian hearts, optical mapping is 43 often used to investigate the interplay between voltage and calcium dynamics and to investigate their 44 respective roles in arrhythmogenesis. This report outlines the construction of an optical mapping 45 system for use with zebrafish hearts, using the voltage-sensitive dye RH 237 and the calcium indicator 46 dye Rhod-2 using two industrial-level CCD cameras. Using economical cameras and a common 532 nm 47 diode laser for excitation, the rate dependence of voltage and calcium dynamics within the atrial and 48 ventricular compartments can be simultaneously determined. At 140 bpm, the atrial action potential 49 duration was 36 ms and the transient duration was 53 ms. Using a programmable electrical 50 stimulator, a shallow rate dependence of 3 and 4 ms per 100 bpm was observed, respectively. In the 51 ventricle the action potential duration was 109 ms and the transient duration was 124 ms, with a 52 steeper rate dependence of 12 and 16 ms per 100 bpm. Synchronous electrocardiograms and optical 53 mapping recordings were recorded, in which the p-wave wave aligns with the atrial voltage peak and 54 r-wave aligns with the ventricular peak. A simple optical pathway and imaging chamber are detailed 55 along with schematics for the in-house construction of the ECG amplifier and electrical stimulator. 56 Laboratory procedures necessary for zebrafish heart isolation, cannulation and loading are also 57 presented. 58
Stability Analysis of MIMO Microgrid Systems in d–q Axis Platform with Constant Power Loads Based on Popov Absolute Stability Criterion

Abstract Microgrid has several advantages over the conventional utility grid system, thus naturally being the gateway to implement the renewable energy resources. Now it is considered as the primary solution to solve the next generation power demand. However, in practice, it is very challenging to retain the microgrid operational stability due to the dense presence of constant power loads in the system. In this article, we considered a technique of compensating the load side to mitigate the instability issues introduced by CPL. Adopting this technique, a stability analysis was presented here for MIMO (multiple input, multiple output) AC microgrid system in dq axis platform by Popov Absolute Stability Criterion using the sector conditions and Popov plots. Besides the recent researches regarding the microgrid stability techniques and the previous analysis using Popov Absolute Stability Criterion, the modeling of the energy storage-based load side compensated microgrid system is also provided in this article. All the cases and results are rigorously scrutinized in virtual platform such as MATLAB/Simulink and verified through experimental results as well.
New Refractive Index Sensor Depend on Resonance Effect

: In this paper, the new refractive index sensor depend on resonance effect is presents. By combine the fiber optic and radio frequency, the sensor could support the most important sensing applications with high accuracy. The optical fiber could be enhanced by side polished fiber to produce a passing filed sensing functions. The zinc oxide film generates high attenuation effect which allows highly sensitive index. By RF magnetron supporting technique, the zinc oxide has been prepared practically with the fiber sensing thickness of 75. 1µm and zinc film of 70 nm has deposited on the polish surface. In refractive index range from 1.3 to 1.4, the achieved sensitivity is 3721.9 nm/RIU. Additionally, all situations were simulated in MATLAB imitation of refractive index to perform the near infrared and visible wavelength. The results show the sensor sensitivity is 0.899 nm for each salt unit.
Comparison of QTLs for adaptive traits between oak and chestnut based on an EST consensus map

A comparative genetic and QTL mapping was performed between Quercus L. and Castanea sativa Mill. , two major forest tree species belonging to the Fagaceae family. Oak EST derived markers (STSs) were used to align the 12 linkage groups of the two species. Fifty-one and 45 STSs were mapped in oak and chestnut, respectively. These STSs, added to SSR markers previously mapped in both species, provided a total number of 55 orthologous molecular markers for comparative mapping within the Fagaceae family. Homeologous genomic regions identified between oak and chestnut allowed to compare QTL position for three important adaptive traits. Co-location of the QTLs controlling the timing of bud burst was significant between the two species. However, conservation of QTLs for height growth was not supported by statistical tests. No QTL for carbon isotope discrimination was conserved between the two species. Putative candidate genes for bud burst can be identified on the basis of co-locations between EST derived markers and QTLs.
A review on emerging applications of emulgel as topical drug delivery system

Topical drug delivery is a convenient mode of drug delivery to treat localized infections. Topical medications are available in many dosage forms, such as creams, ointments, gels, pastes, and lotions. Both emulsions and gels are known for their benefits as topical preparations with few limitations. The literature on emulgel formulations was searched in June 2021 from various scientific journal articles. From a total of 102 searched articles, 24 duplicated articles and 36 irrelevantly judged on the abstract or full papers were excluded. Finally, 47 articles were selected for review. Emulgel possesses many promising properties for dermatological use such as being greaseless, easily removable, easily spreadable, emollient, non-staining, longer shelf-life, transparent, having an elegant appearance and having less potential to cause serious side-effects. Many formulation scientists have started to develop emulgel using various active pharmaceutical ingredients, especially which are hydrophobic in nature. We conclude that formulated emulgels have shown excellent results in aspects such as appearance, rate of drug penetration to skin, rate of drug release and therapeutic response. This review article is mainly focused on formulation, ingredients, methods, and recent developments in emulgel formulations.
A Semantic-Based Similarity Measure for Human Druggable Target Proteins

—The target identiﬁcation is the ﬁrst step on drug discovery pipeline. Thus, techniques which address the selection of potential “druggable targets” and potential “therapeutical targets” are very relevant to the discovery of new drugs and therapies. Nowadays, public databases with drug target indication provide target similarity searching based on BLAST. We demonstrate that the current protein annotation terms may be used on the development of semantic-based measures to provide target similarity searching. This approach allows to predict target similarities based on known signatures of a given protein even without the knowledge of the whole sequence. Our method produces a semantic ordering of the drug targets and provides a tool for latent information retrieving and for clustering analysis. New candidates may be compared against the known targets in a reduced space vector deﬁned by singular value decomposition.
to accelerate genome-to-phenome with the Functional Annotation of Genomes project.

We describe the organization of a nascent international effort, the Functional Annotation of Animal Genomes (FAANG) project, whose aim is to produce comprehensive maps of functional elements in the genomes of domesticated animal species. Predictive biology: from sequence to consequence Most phenotypes are complex and quantitative in nature, and a major goal of biological research lies in using genome information to predict such complex outcomes, whether it is the efficacy of a drug, susceptibility to can-cer, or the performance of the daughters of an elite dairy bull. Many of the recent advances in biology have been driven by genome sequence information. The capability to sequence and decipher the instructions encoded in complex animal genomes quickly and at modest cost is now well established. The next challenge is to be able to read the subtlety and complexity of these instructions
Volume stabilization via α′ corrections in type IIB theory with fluxes

: We consider the type IIB string theory in the presence of various extra 7 = 7-brane pairs compactiﬂed on a warped Calabi-Yau threefold that admits a conifold singularity. We demonstrate that the volume modulus can be stabilized perturbatively at a non-supersymmetric AdS 4 =dS 4 vacuum by the eﬁective potential that includes the stringy ( ﬁ 0 ) 3 correction obtained by Becker et al. together with a combination of positive tension and anomalous negative tension terms generated by the additional 7-brane-antibrane pairs.
SYNTHESIS AND ANTIMICROBIAL ACTIVITY STUDIES OF 3-(7-HYDROXY-4-METHYL-2-OXO-2H-CHROMEN-8-YL)-1-H-PYRAZOLE-4-CARBALDEHYDE COUMARIN DERIVATIVES

Synthesis, spectral analysis and Antimicrobial activity of new coumarin derivatives are described in this paper. Eight new coumarin derivatives were synthesized in moderate to good yields by N,N-Dimethylformamide and POCl 3 via Vilsmeier-Haack Reaction method. The structures of all the newly synthesized molecules were assigned by elemental analysis and spectral data. The synthesized compounds were screened for their Antimicrobial activities strains using Cup plate method.
Regression Test Suite Minimization using Set Theory

-Regression testing is a software maintenance activity which is performed to ensure the proper functionality of the software. The test suits developed during the development phase are too large and exorbitant that it is not possible to re-run the entire test suite in the given time and constraints. Thus it is advisable to choose certain test cases and prioritised them with the objective to undersize the whole test suite dimension. In this paper, we use the concept of Set theory for test case minimization from a larger test suite. The proposed technique can be proved to produce better results.
MSC-derived miR-181b Overexpressing Exosomes Enhanced Osteointegration by Enhancing M2 Polarization of Macrophages via Targeting the PRKCD/AKT Pathway

Wei Liu Second Military Medical University Muyu Yu Shanghai Jiao Tong University Feng Chen Shanghai Jiao Tong University Dong Xie Peking University People's Hospital Longqing Wang East Region Military Command General Hospital Cheng Ye Second Military Medical University Qi Zhu Second Military Medical University Mingzhe Shao Shanghai Jiao Tong University Lili Yang (  yangll@smmu.edu.cn ) Second Military Medical University https://orcid.org/0000-0001-8764-2177
Relationships between abnormal neural activities and cognitive impairments in patients with drug-naive first-episode schizophrenia

Background Prior resting state functional magnetic resonance imaging studies via the regional homogeneity (ReHo) method have demonstrated inconsistent and conflicting results because of several confounding factors, such as small sample size, medicinal influence, and illness duration. Relationships between ReHo measures and cognitive impairments in patients with drug-naive first-episode schizophrenia (dn-FES) are rarely reported. This study was conducted to explore the correlations between ReHo measures, cognitive deficits, and clinical symptoms in patients with dn-FES. Methods A total of 69 patients with dn-FES and 74 healthy controls were recruited. MATRICS Consensus Cognitive Battery (MCCB), Wechsler Adult Intelligence Scale (WAIS), and Positive and Negative Syndrome Scale (PANSS) were used to assess cognitive function, intelligence quotient (IQ), and clinical symptoms, respectively. The correlations between ReHo map and cognitive deficits, the severity of symptoms were examined using strict correlation analyses within brain areas. Results The ReHo values in the right middle frontal gyrus (MFG) and the superior frontal gyrus (SFG) increased in the dn-FES group, whereas the ReHo values in the right cuneus decreased. Correlation analysis showed that the ReHo values in the right MFG were positively correlated with attention/vigilance impairments, social cognition deficits, the severity of clinical manifestations. Conclusions These findings suggested that abnormal spontaneous activities in the right MFG reflect the illness severity and cognitive deficits, which serves as a basis for establishing the objective diagnostic markers and might be a clinical intervention target for patients with schizophrenia.
Assessing the Bioprintability of Self-Assembling Peptide Bioinks in Terms of Structure Fidelity and Cell Viability

(cid:178) Organ donor shortage as well as an increasing demand for personalized medicine have opened up new avenues in tissue engineering. As 3D bioprinting may provide promising solutions, bioinks of different compositions are being developed to serve bioprinting needs. As for the development of suitable bioinks, certain challenges and limitations still exist including: The use of inorganic, unnatural or undefined natural materials, UV and chemical crosslinking for gelation, and fidelity of 3D structures. Self-assembling peptides boast an advantage of resembling human-like materials and activating instantaneous gelation. In this paper, ultrashort peptides are used for 3D bioprinting. The printed scaffolds are analyzed for structure fidelity, cell viability, and proliferation. The results a(cid:85)e compa(cid:85)ed (cid:90)i(cid:87)h comme(cid:85)cial Biogel(cid:91)(cid:140) pep(cid:87)ide bioink(cid:86) a(cid:86) a benchmark. Our custom-designed robotic 3D bioprinter is used and compared with the commercial Inkredible+ bioprinter. Our results prove the bioprintability of self-assembling peptide IK6 (Ac-ILVAGK-NH 2 ) with enhanced cell viability and structure fidelity. Importantly, our results clearly demonstrate the potential use of Self-Assembling peptides as superior bioinks for various tissue engineering applications. Herein, we report a preliminary study on ultrashort peptide-based hydrogels using a custom-developed 3D bioprinting system. The study aims to assess and compare the bioprinting performance of different peptide bioinks, i.e. an ultrashort hexameric peptide-based hydrogel, IK6 (Ac-ILVAGK-NH ),with a commercially available peptide the structure fidelity, cell and cell of each 3D printed construct different Moreover, the comparison of the bioinks was performed two different 3D bioprinting our in-house developed robotic 3D bioprinter