Miracle-dz/UniFAR-FTUs huggingface dataset:
Human Umbilical Cord Blood is a Functionally and Developmentally Relevant Source of Macrophages with Therapeutic Potential

Recently, two major themes have emerged in the field of macrophage biology: developmental origin and self-renewal potential. While the overlap between these two themes remains elusive, recent mouse data demonstrate distinct functions between macrophages generated via primitive hematopoiesis versus definitive hematopoiesis. As for self-renewal potential, terminally differentiated mouse macrophages proliferate via a self-renewal gene regulatory network. Yet, how these observations are associated with each other, and how they extend to human macrophages remain poorly understood. Herein, we hypothesize that human umbilical cord blood (hUCB) is an alternative source of macrophages distinct from human peripheral blood mononuclear cell-derived macrophages (hPBMC MΦ). First, we demonstrate that macrophage-specific functions, such as phagocytosis, reactive oxygen species (ROS) production, and cytokine secretion are comparable between hPBMC MΦ and hUCB MΦ. In contrast, hUCB MΦ are distinct from hPBMC MΦ with respect to primitive hematopoiesis and self-renewal-associated genes. We highlight significantly stronger correlations between these genes in hUCB MΦ, by disrupting
Perancangan Model Data Flow Diagram Untuk Mengukur Kualitas Website Menggunakan Webqual 4.0

The more competition the e-commerce company and the development of technology company in Indonesia, website of Zalora Indonesia traffic rank declining. Measuring the quality of website by using WebQual 4.0 will help manage the web to be able to adjust the quality of the web with user perception. The research aims to make design of Data Flow Diagram model to measure website quality using WebQual 4.0 based on user satisfaction variable. A case study was conducted on the Zalora Indonesia website. Data Flow model is used to make design of system model recommendation, while WebQual 4.0 method is used to measure website quality to user satisfaction. The research data using primary data in the form of questionnaires involving 384 respondents in the city of Bandung who had transacted on the website Zalora Indonesia. Data analysis technique applies descriptive analysis. Based on the research result on the quality of the website Zalora Indonesia, simultaneous positive and significant impact on user satisfaction Zalora Indonesia website. t test result showed that three variables partially have a posotive impact on user satisfaction Zalora Indonesia website is usability quality, information quality and service interaction quality, with Information quality variable has largest impact. Therefore, the modeling system using the Context Diagram-Data Flow Diagram focused on information quality variable.
Insecticidal counting dataset based on one solar insecticidal lamp and two cameras

COPYRIGHT © 2022 Huang, Shu, Li, Feng, Jiang and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. TYPE Data Report PUBLISHED 18 October 2022 DOI 10.3389/fpls.2022. 995118
Principal component analysis of salivary cytokines and hormones in the acute stress response

The acute stress response is characterized by activation of multiple interconnected systems in the body, resulting in the release of a flood of hormones and immune mediators into circulation. In addition to detection of these molecules in the serum, saliva can serve as a source of these markers as well and can be collected in a non-invasive way. The complete profile of salivary biomarkers associated with the hypothalamic pituitary adrenal/gonadal axes and the immune system during the acute stress response has not been fully elucidated. In a cohort of 62 first responders engaged in a stress training exercise, we set out to determine patterns of cytokine, chemokine and hormone shifts during the acute stress response. Salivary samples were collected immediately before (pre-stress), immediately after (post-stress) and 1 h after the stress test (recovery). Multiplex ELISA panels of 42 cytokines and 6 steroid and thyroid hormones were used to determine concentrations of these biomarkers during the three aforementioned time points. Principal components analysis was conducted to determine patterns in the large data sets collected. In our ≥0.3 loading principal components analysis, for pre-stress vs. post, post-stress vs. recovery and pre-stress vs. recovery, a total of three, four and three factors accounted for 56.6, 68.34, and 61.70% of the biomarker variation for each phase respectively. In the ≥0.7 loading principal components analysis, three, four and three factors were found for pre-stress vs. post, post-stress vs. recovery and pre-stress vs. recovery stages, respectively. Of note, in our ≥0.3 loading principal components analysis, MCP1 was present in all three factors from pre-stress to post-stress, and fractalkine was found to be in all four factors post-stress vs. recovery and pre vs. recovery from stress. Additionally, hormones testosterone, estradiol, T4 and T3 grouped together consistently in the same factor for all phases of acute stress in both ≥0.3 and ≥0.7 principal components analysis. Overall, our results identified specific patterns of immune markers and hormones that shift during acute stress and warrant further investigation to understand their mechanistic role in regulating the stress response.
OneArmPhaseTwoStudy: An R Package for Planning, Conducting, and Analysing Single-Arm Phase II Studies

In clinical phase II studies, the efficacy of a promising therapy is tested in patients for the first time. Based on the results, it is decided whether the development programme should be stopped or whether the benefit-risk profile is promising enough to justify the initiation of large phase III studies. In oncology, phase II trials are commonly conducted as single-arm trials with planned interim analyses to allow for an early stopping for futility. The specification of an adequate study design that guarantees control of the type I and II error rates is a key task in the planning stage of such a trial. A variety of statistical methods exists which can be used to optimise the planning and analysis of such studies. However, there are currently neither commercial nor non-commercial software tools available that support the practical application of these methods comprehensively. The R package OneArmPhaseTwoStudy was implemented to fill this gap. The package allows determining an adequate study design for the particular situation at hand as well as monitoring the progress of the study and evaluating the results with valid and efficient analyses methods. This article describes the features of the R package and its application.
The genome of single-petal jasmine (Jasminum sambac) provides insights into heat stress tolerance and aroma compound biosynthesis

Jasmine [Jasminum sambac (L.) Aiton] is a commercially important cultivated plant species known for its fragrant flowers used in the perfume industry, medicine and cosmetics. In the present study, we obtained a draft genome for the J. sambac cultivar ‘Danbanmoli’ (JSDB, a single-petal phenotype). We showed that the final genome of J. sambac was 520.80 Mb in size (contig N50 = 145.43 kb; scaffold N50 = 145.53 kb) and comprised 35,363 genes. Our analyses revealed that the J. sambac genome has undergone only an ancient whole-genome duplication (WGD) event. We estimated that the lineage that has given rise to J. sambac diverged from the lineage leading to Osmanthus fragrans and Olea europaea approximately 31.1 million years ago (Mya). On the basis of a combination of genomic and transcriptomic analyses, we identified 92 transcription factors (TFs) and 206 genes related to heat stress response. Base on a combination of genomic, transcriptomic and metabolomic analyses, a range of aroma compounds and genes involved in the benzenoid/phenylpropanoid and terpenoid biosynthesis pathways were identified. In the newly assembled J. sambac genome, we identified a total of 122 MYB, 122 bHLH and 69 WRKY genes. Our assembled J. sambac JSDB genome provides fundamental knowledge to study the molecular mechanism of heat stress tolerance, and improve jasmine flowers and dissect its fragrance.
Utilisation of injectable flurochromes for quantification of bone growth in a sheep tibial dynamic fixation model

This project examined the use of an augmented histological technique for bone tissue using fluorochrome labelling in a newly developed experimental sheep fracture model. The fracture model encompassed the surgical creation of an experimental fracture which was precisely controlled and independent of external mechanical forces in order to study the effect of the mechanical environment on fracture healing. This research outlined the development and a pilot study combining sequential fluorochrome labelling into the experimental fracture model as a means to enhance the histological analysis of fracture healing and guide development of improved fracture fixation and tissue engineering strategies.
Design and implementation of multiuser mimo precoding algorithms

Los sistemas multiple-input multiple-output (MIMO) se han convertido en la tecnologia clave para los estandares de comunicacion de banda ancha. La principal presente en los sistemas MIMO multiusuario reside en el reto de habilitar la adquisicion de datos no cooperativa en los terminales receptores. Las tecnicas de precodificacion, tales como VP (del ingles vector precoding) realizan una etapa de pre-ecualizacion en la estacion base, de tal manera que la senal en cada receptor se pueda interpretar independientemente y sin el conocimiento del estado general del canal. La principal consecuencia de la incorporacion de un vector de perturbacion en las tecnicas de VP es una considerable mejora en el rendimiento con respecto a los metodos de precodificacion lineales. Esta tesis aborda las dificultades que se derivan del proceso de perturbacion en sistemas VP desde una perspectiva tanto teorica como de implementacion practica.
Optimizing interactions to protein binding sites by integrating docking-scoring strategies into generative AI methods

The identification and optimization of promising lead molecules is essential for drug discovery. Recently, artificial intelligence (AI) based generative methods provided complementary approaches for generating molecules under specific design constraints of relevance in drug design. The goal of our study is to incorporate protein 3D information directly into generative design by flexible docking plus an adapted protein-ligand scoring function, thereby moving towards automated structure-based design. First, the protein-ligand scoring function RFXscore integrating individual scoring terms, ligand descriptors, and combined terms was derived using the PDBbind database and internal data. Next, design results for different workflows are compared to solely ligand-based reward schemes. Our newly proposed, optimal workflow for structure-based generative design is shown to produce promising results, especially for those exploration scenarios, where diverse structures fitting to a protein binding site are requested. Best results are obtained using docking followed by RFXscore, while, depending on the exact application scenario, it was also found useful to combine this approach with other metrics that bias structure generation into “drug-like” chemical space, such as target-activity machine learning models, respectively. Graphical Abstract
Association Between Marginal Jawbone Loss and Onset of Rheumatoid Arthritis and Relationship to Plasma Levels of RANKL

To investigate whether periodontitis, characterized by marginal jawbone loss, precedes the onset of symptoms of rheumatoid arthritis (RA), and to analyze plasma levels of RANKL (a cytokine that is crucial for bone resorption) and anti–citrullinated peptide antibodies (ACPAs) in presymptomatic individuals compared with matched referent controls.
Luteoloside Inhibits IL-1β-Induced Apoptosis and Catabolism in Nucleus Pulposus Cells and Ameliorates Intervertebral Disk Degeneration

Intervertebral disk degeneration (IDD) is the major cause of low back pain (LBP), which affects 80% of the world’s population. Interleukin 1 beta (IL-1β) is a major inflammatory factor that accelerates disk degeneration, and IL-1β levels increase in degenerative disks. It has recently been reported that luteoloside—a type of flavonoid glycoside—has anti-inflammatory properties. In the present study, we investigated the protective potential of luteoloside in IDD. We found that luteoloside maintains cell morphology and inhibits apoptosis (indicated by the reduced expression of cleaved caspase 3) in IL-1β-treated nucleus pulposus (NP) cells. It also suppresses inflammatory mediators—nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), cyclooxygenase 2 (COX-2), and inducible nitric oxide synthase (iNOS)—in IL-1β-treated NP cells. Furthermore, we found increased collagen II and aggrecan expression and reduced MMP13 and ADAMTS5 expression in luteoloside-treated NP cells in the presence of IL-1β. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is involved in apoptosis, inflammation, and extracellular matrix (ECM) homeostasis. Mechanistic studies revealed that the NF-κB signaling pathway is inhibited by luteoloside, and Nrf2 is involved in the regulation of luteoloside in NF-κB signaling because Nrf2 knockdown reduced the suppressive effect of luteoloside on NF-κB signaling. We also established a puncture-induced rat IDD model and demonstrated that the persistent intraperitoneal injection of luteoloside ameliorates the progression of IDD. In conclusion, we demonstrated that luteoloside activates the Nrf2/HO-1 signaling axis and is a potential therapeutic medicine for IDD.
Serum Levels of Soluble Fractalkine in Patients with Systemic Lupus Erythematosus

Background: Fractalkine (Fkn)/CX3CL1 which is a unique member of the CX3C chemokine subfamily, and expressed on inflamed endothelium appears to possess immunoregulatory properties that affect inflammatory/immune cell interactions and inflammatory responses at sites of inflammation. Objective: The purpose of the present study was to determine the Fractalkine/CX3CL1 level in SLE patients and correlates that level with indices of disease activity and damage, trying to disclose its role in the pathogenesis of SLE. Methods: The study was carried on forty SLE patients (classified into 15 active and 25 inactive by using clinical and the BILAG disease activity index assessment), thirty patients with rheumatoid arthritis (RA) as disease control group and twenty healthy as control group. Levels of soluble Fkn were measured by enzyme-linked immunosorbent assay. Expression of Fkn /CX3CL1 was quantified by real-time polymerase chain reaction. Results: Both serum sFkn levels and mRNA expression of Fkn /CX3CL1 were significantly higher in patients with SLE compared with RA patients and healthy controls (P<0.05) and were significantly higher in SLE patients with active disease than in those with inactive disease. Also, serum levels of sFkn were positively correlated with disease activity, organ damage, anti–double-stranded DNA (anti-dsDNA) antibody titers, anti-Sm antibody titers, immune complex C1q levels, anti-phospholipid, anti-RNP and ESR level and negatively correlated with total hemolytic complement activity (CH50). Conclusion: sFkn and CX3CL1 mRNA expression play crucial roles in the pathogenesis of SLE and that sFkn may serve as a serologic inflammatory marker of disease activity and organ damage. Key wards: Soluble Fractalkine (sFkn); Fractalkine (Fkn)/CX3CL1; SLE; Disease activity.
Interference Analysis of Massive MIMO Downlink with MRT Precoding and Applications in Performance Analysis

Recently, massive MIMO (multiple-input-multiple-output) systems, where the base station (BS) is equipped with hundreds of small, low-cost, and low-power antennas, have been proposed as one of the promising technologies for the next generation cellular systems. The thesis works on the performance analysis of single-cell multi-user massive MIMO downlink. Perfect channel state information (CSI) is assumed at the BS and maximum ratio transmission (MRT) precoding scheme is adopted. We first investigate the distribution of the interference power and derive its probability density function (pdf) by central limit theory. After that, analytical results on the outage probability and the sum-rate are derived. Different to existing work using the law of large numbers to derive the asymptotic deterministic signal-to-interference-plusnoise-ratio (SINR), the randomness of the interference in the SINR is kept intact in our work, which allows the derivation of the outage probability. We further extend to networks with per-antenna power constraint. A modified MRT precoding scheme is proposed and the performance of the modified scheme is analyzed. Our work show that the modified MRT precoding can achieve lower outage probability and higher sum-rate than MRT precoding, even with more strict power constraint.
Smartphone Use Predicts Being an “Active Couch Potato” in Sufficiently Active Adults

Purpose: To investigate the relationship between smartphone use, television viewing, and the “active couch potato” in sufficiently active adults. Methods: A sample of 328 sufficiently active adults aged 18 to 80 years (mean ± SD = 38 ± 15 years) completed surveys assessing smartphone use, television viewing, physical activity, and sedentary behavior. Active couch potatoes were defined as individuals who met physical activity guidelines and engaged in 8 or more hours per day of sedentary behavior. Results: Mean smartphone use was 4.04 ± 3.74 hours per day. There were 128/328 (39%) participants coded as active couch potatoes. Active couch potato was significantly positively (Wald = 7.326, P = .007) associated with smartphone use. Being an active couch potato was not (Wald = 0.658, P = .417) associated with television viewing. Sedentary behavior and smartphone use were significantly greater (t ≥ 3.55, P < .001) among active couch potatoes (11.35 ± 3.25 hours sitting per day, 4.95 ± 4.5 hours smartphone use per day) than sufficiently active, nonsedentary individuals (5.06 ± 1.64 hours sitting per day, 3.45 ± 3.04 hours smartphone use per day). Conclusion: In sufficiently active adults, smartphone use predicted being an active couch potato and television viewing did not.
Facial Expression Recognition Method Based on Stacked Denoising Autoencoders and Feature Reduction

Based on the deep learning theory, a novel facial expression recognition method, which utilizes both Principal Component Analysis (PCA) and stacked denoising autoencoders (SDAE), is proposed in this paper. At first, PCA is used as a linear dimensionality reduction method on the expression features, and subsequently non-linear dimensionality is further learnt by a greed layer-wise method of stacked denoising autoencoders. Then, some meaningful and low-dimensioned expression features can be learnt and used to classify. The comparative experiment results show that the proposed method is more effective than some other expression recognition methods based on deep learning theories and it can also get higher expression recognition accuracy than traditional non-deep learning based expression recognition methods.
Cardiometabolic Risk Profile Among Young Adult Females With a History of Premature Adrenarche

Abstract Context Premature adrenarche (PA) is associated with childhood overweight and hyperinsulinemia; the long-term cardiometabolic outcome is unknown. Objective To study cardiometabolic profile in adult women with previous PA. Design and participants Thirty women with PA and 41 control subjects were followed from prepuberty to young adulthood. Main outcome measures Prevalence of the metabolic syndrome (MetS) and clinical and biochemical cardiovascular risk factors. Results There were no differences in the prevalence of MetS or in any parameters indicating dyslipidemia, hypertension, hepatosteatosis, atherosclerosis, or low-grade inflammation between the study groups. However, prevalence of insulin resistance (IR; P = 0.014) and acanthosis nigricans (P = 0.010) was higher in the PA group. Neither fasting glucose nor insulin concentrations differed between the study groups, but HbA1c [adjusted for body mass index (BMI) P = 0.011] and Homeostatic Model Assessment of Insulin Resistance (P = 0.044; BMI-adjusted P = nonsignificant) were higher in the PA group. Although BMI and fat percentage were comparable between the study groups, the PA group had higher central fat mass than the control group. In the whole study population, MetS and IR were associated with greater adult fat mass, but no prepubertal factors predicting later IR were found. Conclusion PA does not seem to be associated with MetS, dyslipidemia, hypertension, atherosclerosis, or low-grade inflammation in young adult women. However, some women with PA may be at an increased risk of unfavorable glucose metabolism, which is associated with increased central adiposity at adult age rather than determined by prepubertal factors.
Multimodal Targets in Speech Production: Acoustic, Articulatory and Dynamic Eevidence from Formant Perturbation

Multimodal targets in speech production: Acoustic, articulatory and dynamic evidence from formant perturbation Chris Neufeld Masters of Science Graduate Department of Speech Language Pathology University of Toronto 2013 This thesis presents evidence from a formant perturbation experiment which supports the hypothesis that speech targets are multimodal. A real-time auditory feedback perturbation is used to gradually shift English speakers’ formants from the vowel /E/ towards /I/. Most speakers compensate at the level of acoustics, adjusting their production towards /æ/ such that they hear themselves producing the correct vowel. Subjects’ articulation is tracked with electromagnetic-articulography. The articulatory data shows that subjects tend to produce marginal /E/s at the level of articulation remaining within the normal articulatory bounds for that vowel, while adjusting the position of individual articulators to a sufficient extent to create an acoustic compensation to the perturbation. The higher-order relationship between speed and curvature is shown to differ across different vowel phonemes. However, this measure remains constant under formant perturbation. These findings are argued to show that phonemic targets are multi-modal, having acoustical, kinematic, and dynamic components.
Use of Imaging Techniques to Assess Skin Wound Healing for Tissue Engineering and Regenerative Medicine

Citation: Feldman D (2018) Use of Imaging Techniques to Assess Skin Wound Healing for Tissue Engineering and Regenerative Medicine. Int J Biol Instrum 1:002 Copyright: © 2018 Feldman D. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. *Corresponding author: Dale Feldman, PhD, Department of Biomedical Engineering, University of Alabama, 361 HOEN, AL 35294, Birmingham, USA, Tel: 205-934-8426, Fax: 205-975-4919, E-mail: dfeldman@uab.edu Received: September 01, 2017: Accepted: March 20, 2018: Published: March 22, 2018 VIBGYOR ISSN: 2631-5025