Miracle-dz/UniFAR-FTUs huggingface dataset:
Temperature dependency of predation: Increased killing rates and prey mass consumption by predators with warming

Abstract Temperature dependency of consumer–resource interactions is fundamentally important for understanding and predicting the responses of food webs to climate change. Previous studies have shown temperature‐driven shifts in herbivore consumption rates and resource preference, but these effects remain poorly understood for predatory arthropods. Here, we investigate how predator killing rates, prey mass consumption, and macronutrient intake respond to increased temperatures using a laboratory and a field reciprocal transplant experiment. Ectothermic predators, wolf spiders (Pardosa sp.), in the lab experiment, were exposed to increased temperatures and different prey macronutrient content (high lipid/low protein and low lipid/high protein) to assess changes in their killing rates and nutritional demands. Additionally, we investigate prey mass and lipid consumption by spiders under contrasting temperatures, along an elevation gradient. We used a field reciprocal transplant experiment between low (420 masl; 26°C) and high (2,100 masl; 15°C) elevations in the Ecuadorian Andes, using wild populations of two common orb‐weaver spider species (Leucauge sp. and Cyclosa sp.) present along the elevation gradient. We found that killing rates of wolf spiders increased with warmer temperatures but were not significantly affected by prey macronutrient content, although spiders consumed significantly more lipids from lipid‐rich prey. The field reciprocal transplant experiment showed no consistent predator responses to changes in temperature along the elevational gradient. Transplanting Cyclosa sp. spiders to low‐ or high‐elevation sites did not affect their prey mass or lipid consumption rate, whereas Leucauge sp. individuals increased prey mass consumption when transplanted from the high to the low warm elevation. Our findings show that increases in temperature intensify predator killing rates, prey consumption, and lipid intake, but the responses to temperature vary between species, which may be a result of species‐specific differences in their hunting behavior and sensitivity to temperature.
The role of the gene Egalitarian in Drosophila oocyte determination and polarity

Development can be described as the process by which cells become different from their siblings. The Drosophila oocyte forms from one of sixteen interconnected sister cells; the other fifteen cells form polyploid feeder cells known as nurse cells. In egalitarian (egl) mutants, the cell that should become the oocyte instead follows the same fate as its sister cells, forming a sixteenth nurse cell. The function of the egl gene is required in the oocyte and nurse cells of the ovary and egl mRNA and protein localize to the developing oocyte very early in oogenesis. Three lines of evidence show that egl interacts with BicaudalD (BicD), another gene required for oocyte determination. First, the Egl and BicD proteins colocalize within the egg and this localization requires the function of both genes. Second, the penetrance of BicD dominant mutations can be altered by changing the egl gene dosage, showing a genetic interaction between the loci. Third, Egl and BicD proteins can be coimmunoprecipitated from ovary extracts, indicating that the two proteins form part of a protein complex. The polarity of the oocyte forms in several steps, each of which requires the polarized microtubule cytoskeleton. Early in oogenesis, microtubules emanate from a microtubule organizing center (MTOC) at the posterior cortex of the oocyte. At this stage, microtubules are essential for oocyte determination; later in oogenesis, the location of the MTOC shifts to the anterior cortex of the oocyte and microtubules are essential for the dorso-ventral polarity of the oocyte. Egl protein is localized in a pattern reminiscent of the location of the minus ends of microtubules and Egl protein localization requires microtubules. Also, egl mutants expressing low amounts of egl produce eggs that lack dorsal structures, indicating that egl is also required for the dorso-ventral polarity of the oocyte. egl function may provide a link between microtubule function in oocyte determination and RNA localization in oocyte axis formation. Indeed, the elaboration of the oocyte/nurse cell axis may be the first step in the iterative elaboration of polarity in the Drosophila oocyte Thesis supervisor: Ruth Lehmann Title: Associate Professor of Biology
Spatial grain of adaptation is much finer than ecoregional‐scale common gardens reveal

Abstract Adaptive variation among plant populations must be known for effective conservation and restoration of imperiled species and predicting their responses to a changing climate. Common‐garden experiments, in which plants sourced from geographically distant populations are grown together such that genetic differences may be expressed, have provided much insight on adaptive variation. Common‐garden experiments also form the foundation for climate‐based seed‐transfer guidelines. However, the spatial scale at which population differentiation occurs is rarely addressed, leaving a critical information gap for parameterizing seed‐transfer guidelines and assessing species’ climate vulnerability. We asked whether adaptation was evident among populations of a foundational perennial within a single “empirical” seed‐transfer zone (based on previous common‐garden findings evaluating very distant populations) but different “provisional” seed zones (groupings of areas of similar climate and are not parameterized from common‐garden data). Seedlings from three populations originating from similar conditions within an intermediate elevation were planted into gardens nearby at the same elevation, or 250–450 m higher or lower in elevation and 0.4–25 km away. Substantial variation was observed between gardens in survival (ranging 2%–99%), foliar crown volume (7.8–22.6 dm3), and reproductive effort (0%–65%), but not among the three transplanted populations. The between garden variation was inversely related to climatic differences between the gardens and seed‐source populations, specifically the site differences in maximum–minimum annual temperatures. Results suggest that substantial site‐specificity in adaptation can occur at finer scales than is accounted for in empirical seed‐transfer guidance when the guidance is derived from broadscale common‐garden studies. Being within the same empirical seed zone, geographic unit, and even within 10 km distance may not qualify as “local” in the context of seed transfer. Moving forward, designing common‐garden experiments so that they allow for testing the scale of adaptation will help in translating the resulting seed‐transfer guidance to restoration projects.
Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis

Recognition of conserved microbial molecules activates immune responses in plants, a process termed pattern-triggered immunity (PTI). Similarly, insect eggs trigger defenses that impede egg development or attract predators, but information on the nature of egg-associated elicitors is scarce. We performed an unbiased bioactivity-guided fractionation of eggs of the butterfly Pieris brassicae. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry of active fractions led to the identification of phosphatidylcholines (PCs). PCs are released from insect eggs, and they induce salicylic acid and H2O2 accumulation, defense gene expression and cell death in Arabidopsis, all of which constitute a hallmark of PTI. Active PCs contain primarily C16 to C18-fatty acyl chains with various levels of desaturation, suggesting a relatively broad ligand specificity of cell-surface receptor(s). The finding of PCs as egg-associated molecular patterns (EAMPs) illustrates the acute ability of plants to detect conserved immunogenic patterns from their enemies, even from seemingly passive structures such as eggs.
Identification of NUF2 and FAM83D as potential biomarkers in triple-negative breast cancer

Background Breast cancer is a heterogeneous disease. Compared with other subtypes of breast cancer, triple-negative breast cancer (TNBC) is easy to metastasize and has a short survival time, less choice of treatment options. Here, we aimed to identify the potential biomarkers to TNBC diagnosis and prognosis. Material/Methods Three independent data sets (GSE45827, GSE38959, GSE65194) were downloaded from the Gene Expression Omnibus (GEO). The R software packages were used to integrate the gene profiles and identify differentially expressed genes (DEGs). A variety of bioinformatics tools were used to explore the hub genes, including the DAVID database, STRING database and Cytoscape software. Reverse transcription quantitative PCR (RT-qPCR) was used to verify the hub genes in 14 pairs of TNBC paired tissues. Results In this study, we screened out 161 DEGs between 222 non-TNBC and 126 TNBC samples, of which 105 genes were up-regulated and 56 were down-regulated. These DEGs were enriched for 27 GO terms and two pathways. GO analysis enriched mainly in “cell division”, “chromosome, centromeric region” and “microtubule motor activity”. KEGG pathway analysis enriched mostly in “Cell cycle” and “Oocyte meiosis”. PPI network was constructed and then 10 top hub genes were screened. According to the analysis results of the Kaplan-Meier survival curve, the expression levels of only NUF2, FAM83D and CENPH were associated with the recurrence-free survival in TNBC samples (P < 0.05). RT-qPCR confirmed that the expression levels of NUF2 and FAM83D in TNBC tissues were indeed up-regulated significantly. Conclusions The comprehensive analysis showed that NUF2 and FAM83D could be used as potential biomarkers for diagnosis and prognosis of TNBC.
One-year results after transitioning from etanercept originator to biosimilar in a setting promoting shared decision-making in rheumatology

Abstract Objective The aim was to study the effect of non-mandatory transitioning from etanercept originator to etanercept biosimilar on retention rates in a setting promoting shared decision-making. Methods In 2016, all patients treated with etanercept originator and stable disease at the Rheumatology department in Bernhoven were offered transitioning to etanercept biosimilar by an opt-in approach. A historical cohort of patients treated with etanercept originator in 2015 was identified as the control group. Etanercept discontinuation was compared between the cohorts using Cox regression. To study the nocebo effect, reasons for discontinuation were categorized into objective reasons (e.g. laboratory abnormalities, increase in swollen joint count, allergic reaction) and subjective health complaints (symptoms perceptible only to the patient, e.g. tiredness, arthralgia). An adjusted Kaplan–Meier curve for retention of the etanercept biosimilar was made, censoring subjective health complaints as the reason for discontinuation. Results Seventy of the 79 patients eligible for transitioning agreed to transition (89%). The 1-year crude retention rate of etanercept in the transition cohort was 73% (95% CI: 0.62, 0.83), compared with a retention rate of 89% (95% CI: 0.81, 0.95) in the historical cohort (P = 0.013). This resulted in a higher risk of treatment discontinuation in the transition cohort (adjusted hazard ratio = 2.73; 95% CI: 1.23, 6.05, P = 0.01). After adjusting for the nocebo effect, the cohorts had comparable retention rates (86 vs 89%, P = 0.51). Conclusion Non-mandatory transition from etanercept originator to its biosimilar using an opt-in approach in a setting promoting shared decision-making resulted in a higher discontinuation of etanercept compared with the historical cohort. This could be attributed largely to the nocebo effect.
RAPD Marker Variation in Eriastrum densifolium (Polemoniaceae): Implications for Subspecific Delimitation and Conservation

Eriastrum densifolium is a perennial sub-shrub exhibiting complex morphological variation. Five subspecies are recognized, differentiated by flower dimensions and geographic/elevational distribution. One subspecies, E. d. subsp. sanctorum, is known from a single population and is recognized as a federally endangered taxon. The subspecies are difficult to distinguish and do not adequately represent the total morphological diversity in the species. Variation in the species is examined with Random Amplified Polymorphic DNA markers in an attempt to identify genetically cohesive groups of populations. Random Amplified Polymorphic DNA marker diversity indicates that genetic variation is not partitioned geographi- cally or elevationally in the species, but genetic distance and geographic distance are well correlated. Furthermore, population relationships suggest that the currently recognized subspecies are not natural groups. Eriastrum densifolium subsp. sanctorum possesses relatively low genetic diversity in comparison to close geographic neighbors, is not genetically divergent from other populations, and is not a member of a distinct population group. In general, geographic variation in different Random Amplified Polymorphic DNA markers is discordant, and the resulting pattern suggests a continuum of neutral genetic marker variation in Eriastrum densifolium, with no intraspecific taxa evident.
Limits to the Energy Budget in a Rodent, Peromyscus maniculatus: The Central Limitation Hypothesis

The maximum rate of energy assimilation in lactating Peromyscus maniculatus (100 kJ/d, body mass = 25 g) was lower than in cold-exposed females (115 kJ/d, body mass = 20 g). This pattern differs from that found in a few species of voles and mice, in which lactating females achieve higher rates of assimilation than cold-exposed animals. The results do not support the hypothesis that energy budgets of animals are limited centrally by the rate of energy assimilation from food, irrespective of which metabolic function constitutes the main energy expenditure. The discussion about physiological limits to energy budgets must involve information about the life strategies of the animals.
Seasonal Changes in the Active Bat Community of the Kisatchie National Forest, Louisiana

Abstract Few North American studies have quantified differences in bat community composition between summer and winter. In southerly regions, especially the coastal plain of the Gulf of Mexico, winters are mild and experience only short periods of freezing weather annually. In regions such as this, there may be a substantive community of bats that are active in the winter. We examined seasonality of the bat community in the Kisatchie National Forest of Louisiana. We mist-netted bats for 130 nights during winter and 51 nights during summer and caught 200 and 190 bats, respectively, from 10 different species. Corynorhinus rafinesquii (Rafinesque's Big-eared Bat), Lasiurus borealis (Eastern Red Bat), and Lasionycteris noctivagans (Silver-haired Bat) were more frequently captured in winter, all other species were captured more frequently in summer. Significant differences existed between summer and winter in species richness and abundance of bats, but not for Shannon's diversity index. Across the entire year and in winter, more bats were caught on nights with higher temperature than on nights with lower temperatures. Although there was much temporal variation in species composition, we found a substantial bat community that is active in the winter in the Kisatchie National Forest of Louisiana.
Movements of Juvenile Winter Flounder in a Southern Maine Estuary

Abstract Overfishing and habitat loss have reduced Pseudopleuronectes americanus (Winter Flounder) populations within the Gulf of Maine (GOM), and despite strict management regulations, abundance is still low. As the GOM continues to warm, there is need to characterize Winter Flounder movements in estuaries to better understand essential habitat use. To characterize movements and habitat use of Winter Flounder, we tagged 17 juvenile flounder (115–170 mm) with acoustic transmitters (Vemco V7-2x) and monitored them using passive tracking over a 4-month period between July and November 2017 in the Saco River Estuary (SRE) in southern Maine. Movement within the SRE was highly variable, with cumulative movements of individual tracked flounder varying between 0 m (e.g., detected only at the release site) and 17,016 m. Tagged flounder were most often present in moderate (15–20 °C) temperatures and salinities (18–26 ppt), and were rarely observed in the highest temperatures (>20 °C) and salinities (>26 ppt). Movements upstream and downstream by flounder appeared to be tidally driven, with most downstream movements occurring during ebb tides and upstream movements during incoming tides. In addition, large flounder moved further away from freshwater, while small flounder either stayed within the river mouth's jetties or moved upstream. The speeds of flounder movements among receiver locations (mean = 4.49 body lengths per second, SE = 0.85) were much faster than expected for flatfish, potentially facilitated by use of tides. The GOM populations of Winter Flounder may benefit from management of juvenile habitat as environmental conditions change.
Exosomes derived from Vδ2-T cells control Epstein-Barr virus–associated tumors and induce T cell antitumor immunity

Exosomes from expanded allogeneic Vδ2-T cells kill EBV-associated tumors and promote antitumor immune responses. No holds barred for antitumor attack Epstein-Barr virus (EBV) is a common pathogen that contributes to the development of several cancers, which can be difficult to treat. γδ-T cells have innate activity and have been proposed as a cancer immunotherapy, but they have been difficult to apply, particularly because they have to be individually prepared for each patient. To overcome the hurdles associated with whole γδ-T cell–based therapy, Wang et al. isolated exosomes from a subtype of activated γδ-T cells, which contained multiple immunostimulatory molecules and cell death–inducing ligands. These exosomes were effective against multiple mouse models of EBV-associated cancers and did not require customized preparation unlike cell-based therapies. Treatment of life-threatening Epstein-Barr virus (EBV)–associated tumors remains a great challenge, especially for patients with relapsed or refractory disease. Here, we found that exosomes derived from phosphoantigen-expanded Vδ2-T cells (Vδ2-T-Exos) contained death-inducing ligands (FasL and TRAIL), an activating receptor for natural killer (NK) cells (NKG2D), immunostimulatory ligands (CD80 and CD86), and antigen-presenting molecules (MHC class I and II). Vδ2-T-Exos targeted and efficiently killed EBV-associated tumor cells through FasL and TRAIL pathways and promoted EBV antigen–specific CD4 and CD8 T cell expansion. Administration of Vδ2-T-Exos effectively controlled EBV-associated tumors in Rag2−/−γc−/− and humanized mice. Because expanding Vδ2-T cells and preparing autologous Vδ2-T-Exos from cancer patients ex vivo in large scale is challenging, we explored the antitumor activity of allogeneic Vδ2-T-Exos in humanized mouse cancer models. Here, we found that allogeneic Vδ2-T-Exos had more effective antitumor activity than autologous Vδ2-T-Exos in humanized mice; the allogeneic Vδ2-T-Exos increased the infiltration of T cells into tumor tissues and induced more robust CD4 and CD8 T cell–mediated antitumor immunity. Compared with exosomes derived from NK cells (NK-Exos) with direct cytotoxic antitumor activity or dendritic cells (DC-Exos) that induced T cell antitumor responses, Vδ2-T-Exos directly killed tumor cells and induced T cell–mediated antitumor response, thus resulting in more effective control of EBV-associated tumors. This study provided proof of concept for the strategy of using Vδ2-T-Exos, especially allogeneic Vδ2-T-Exos, to treat EBV-associated tumors.
Cavin3 Suppresses Breast Cancer Metastasis via Inhibiting AKT Pathway

Objective Cavin3 is a putative tumor suppressor protein. However, its molecular action on tumor regulation is largely unknown. The aim of the current study is to explore the implication of cavin3 alteration, its clinical significance, and any potential molecular mechanisms in the regulation of breast cancer (BC). Methods TCGA (The Cancer Genome Atlas) and GTEx (Genotype-Tissue Expression) data bases, and 17 freshly paired BC and adjacent normal tissues were analyzed for mRNA levels of Cavin3. Furthermore, cavin3 protein expression from 407 primary BC samples were assessed by immunohistochemistry (IHC) and measured by H-score. The clinical significance of cavin3 expression was explored by Kaplan-Meier analysis and the Cox regression method. In vitro biological assays were performed to elucidate the function and underlying mechanisms of cavin 3 in BC cell lines. Results Cavin3 mRNA was dramatically down-regulated in BC compared with the negative control. The median H-score of cavin3 protein by IHC was 50 (range 0-270). There were 232 (57%) and 175 (43%) cases scored as low (H-score≤50) and high (H-score >50) levels of cavin3, respectively. Low cavin3 was correlated with a higher T and N stage, and worse distant metastasis-free survival (DMFS) and overall survival (OS). Multivariate survival analysis revealed low cavin3 was an independent fact for worse DMFS. In BC cells, an overexpression of cavin3 could inhibit cell migration and invasion, and significantly decreased the level of p-Akt. Knockout of cavin3, meanwhile, promoted cell invasion ability and increased the level of p-AKT. Conclusion Cavin3 expression is significantly lower in BC and is correlated with distant metastasis and worse survival. Cavin3 functions as a metastasis suppressor via inhibiting the AKT pathway, suggesting cavin3 as a potential prognostic biomarker and a target for BC treatment.
Comparing responses to public health and industry-funded alcohol harm reduction advertisements: an experimental study

Objectives Conduct a head-to-head experimental test of responses to alcohol harm reduction advertisements developed by alcohol industry Social Aspects/Public Relations Organisations (SAPROs) versus those developed by public health (PH) agencies. We hypothesised that, on average, SAPRO advertisements would be less effective at generating motivation (H1) and intentions to reduce alcohol consumption (H2) but more effective at generating positive perceptions of people who drink (H3). Design Online experiment with random assignment to condition. Participants 2923 Australian adult weekly drinkers (49% high-risk drinkers) recruited from an opt-in online panel. Interventions Participants viewed 3 of 83 advertisements developed by PH agencies (n=2174) or 3 of 28 advertisements developed by SAPROs (n=749). Primary outcome measures Participants reported their motivation to reduce the amount of alcohol consumed; behave responsibly and/or not get drunk; and limit their drinking around/never supply to minors, as well as intentions to avoid drinking alcohol completely; reduce the number of drinking occasions; and reduce the amount of alcohol consumed per occasion. Participants also reported their perceptions of people who drink alcohol on six success-related items and four fun-related items. Results Compared with drinkers exposed to PH advertisements, those exposed to SAPRO advertisements reported lower motivation to reduce the amount of alcohol consumed (β=−0.091, 95% CI −0.171 to −0.010), and lower odds of intending to avoid alcohol completely (OR=0.77, 0.63 to 0.94) and to reduce the amount of alcohol consumed per occasion (OR=0.82, 0.69 to 0.97). SAPRO advertisements generated more favourable fun-related perceptions of drinkers (β=0.095, 0.013 to 0.177). Conclusions The alcohol harm reduction advertisements produced by alcohol industry SAPROs that were tested in this study were not as effective at generating motivation and intentions to reduce alcohol consumption as those developed by PH organisations. These findings raise questions as to whether SAPROs should play a role in alcohol harm reduction efforts.