Miracle-dz/UniFAR-FTUs huggingface dataset:
I. Sequence Evolution and Conservation in a Highly Repeated DNA Family’

The restriction enzyme Tag1 digests 0.2% of the genomic DNA from the grasshopper Caledia captiva to a family of sequences 168 bp in length (length of consensus sequence). The sequence variation of this “Taq family” of repeat units was examined among four races from C. captiva to assay the pattern of evolution within this highly repeated DNA. The Tag-family repeats are located in C-banded heterochromatin on at least one member of each homologous pair of chromosomes; the locations range from centromeric to telomeric. Thirty-nine cloned repeats isolated from two population IA individuals along with 11 clones from seven populations taken from three of the races demonstrated sequence variation at 72 positions. Pairwise comparisons of the cloned repeats, both within an individual and between different races, indicate that levels of intraspecific divergence, as measured by reproductive incompatibility, do not correlate with sequence divergence among the 168bp repeats. A number of subsequences within the repeat remain unchanged among all 50 clones; the longest of these is 18 bp. That the same 18-bp subsequence is present in all clones examined is a finding that departs significantly (P < 0.01) from what would be expected to occur at random. Two other cloned repeats, from a reproductively isolated race of C. captiva, have sequences that show 56% identity with this 18-bp conserved region. An analysis showed that the frequency of occurrence of an RsaI recognition site within the 168-bp repeat in the entire Tag family agreed with that found in the cloned sequences. These data, along with a partial sequence for the entire Tag family obtained by sequencing uncloned repeats, suggest that the consensus sequence from the cloned copies is representative of this highly repeated family and is not a biased sample resulting from the cloning procedure. The 18-bp conserved sequence is part of a 42-bp sequence that possesses dyad symmetry typical of protein-binding sites. We speculate that this may be significant in the evolution of the Tag family of sequences.
Groups with many generalized 𝐹 𝐶 -subgroup

Let 𝐹𝐶 0 be the class of all ﬁnite groups, and for each non-negative integer 𝑚 deﬁne by induction the group class 𝐹𝐶 𝑚 +1 consisting of all groups 𝐺 such that the factor group 𝐺/𝐶 𝐺 ( 𝑥 𝐺 ) has the property 𝐹𝐶 𝑚 for all elements 𝑥 of 𝐺 . Clearly, 𝐹𝐶 1 is the class of 𝐹𝐶 -groups and every nilpotent group with class at most 𝑚 belongs to 𝐹𝐶 𝑚 . The class of 𝐹𝐶 𝑚 -groups was introduced in [6]. In this article the structure of groups with ﬁnitely many normalizers of non- 𝐹𝐶 𝑚 -subgroups (respectively, the structure of groups whose subgroups either are subnormal with bounded defect or have the property 𝐹𝐶 𝑚 ) is investigated.
Towards a Methodology for Appropriate Acquisition of Design Software in Concurrent Engineering Design Organisations

Concurrent Engineering (CE) attempts to process as many product development tasks in parallel as possible while at the same time maximising the use of downstream life-cycle knowledge at early stages of the design process. An effective implementation of this strategy can reduce the duration of design projects, save development costs, and provide better quality products. The successful management and execution of CE projects within such a highly distributed, knowledge intensive environment requires an appropriate selection of Knowledge Based Engineering (KBE) software as well as Computer Aided Engineering (CAE) tools for product and process modelling and analysis, and includes tools for such functions as data management, decision-making and communications between stakeholders. Because such tools and technology play a significant role in the successful implementation of CE, an important challenge to modern industry is the ability to quickly and accurately assess and acquire the most appropriate software tools to support product development. A framework that utilises the Analytic Hierarchy Process (AHP) technique and incorporates the unique requirements of the CE product development domain is proposed.
Massive preparation of giant vesicles by squeezing a marshmallow-like silicone gel for accelerating artificial cell researches

by squeezing a silicone gel for artificial cell A simple method of preparation giant vesicles by squeezing out with a buffer from flexible porous monoliths impregnated with lipids was reported. TEXT Vesicles (liposomes), membranes similar to cell and organelle membranes have been used as a simple model for understanding living cell systems and their interfacial functions. Vesicles are indispensable for both elucidation of life phenomena and basic scientific research on topics such as artificial cells, microsynthesis, drug delivery, and cosmetics. 1-3 Various processes have been used to produce vesicles, such as hydration, ultrasonic treatment, membrane emulsification, centrifugal sedimentation, and use of microflow channels. 4-8 Each method has advantages and disadvantages, and thus the process most suitable for a particular purpose is selected. Especially
Signiﬁcance of Light, Sugar, and Amino Acid Supply for Diurnal Gene Regulation in Developing Barley Caryopses

The caryopses of barley ( Hordeum vulgare ), as of all cereals, are complex sink organs optimized for starch accumulation and embryo development. While their early to late development has been studied in great detail, processes underlying the caryopses’ diurnal adaptation to changes in light, temperature, and the ﬂuctuations in phloem-supplied carbon and nitrogen have remained unknown. In an attempt to identify diurnally affected processes in developing caryopses at the early maturation phase, we monitored global changes of both gene expression and metabolite levels. We applied the 22 K Barley1 GeneChip microarray and identiﬁed 2,091 differentially expressed (DE) genes that were assigned to six major diurnal expression clusters. Principal component analysis and other global analyses demonstrated that the variability within the data set relates to genes involved in circadian regulation, storage compound accumulation, embryo development, response to abiotic stress, and photosynthesis. The correlation of amino acid and sugar proﬁles with expression trajectories led to the identiﬁcation of several hundred potentially metabolite-regulated DE genes. A comparative analysis of our data set and publicly available microarray data disclosed suborgan-speciﬁc expression of almost all diurnal DE genes, with more than 350 genes speciﬁcally expressed in the pericarp, endosperm, or embryo tissues. Our data reveal a tight linkage between day/night cycles, changes in light, and the supply of carbon and nitrogen. We present a model that suggests several phases of diurnal gene expression in developing barley caryopses, summarized as starvation and priming, energy collection and carbon ﬁxation, light protection and chaperone activity, storage and growth, and embryo development.
Detection of Mbo II Polymorphism at the 5 * Promoter

pregnancy (5, 9–11). In the case we report, monomeric prolactin increased, but macroprolactin remained the major immunoreactive prolactin component in serum during pregnancy. Given the medical history and subsequent progress of this present patient, it is unlikely that the vast majority of her circulating prolactin (up to 90% macroprolactin) was bioactive. The data presented on this patient confirm that, depending on the assay system used, widely varying estimates of serum prolactin concentrations may be encountered in serum containing macroprolactin. It seems probable that the varying response of assay systems reflects the variable structure of macroprolactin and the availability of epitopes on the prolactin component to react with assay antibodies.
Combining and evaluating regression methods for galaxy redshift estimates

In this thesis I develop a Machine Learning method to combine galactic red-shift estimates of previous authors into an aggregate estimate. I investigate weather combining earlier results into a single estimate is a worthwhile eﬀort. Disagree-ment between the earlier results is used as a metric the quality of estimation. This disagreement is exploited using kernel density estimation to iteratively selecting a subset of the problem that is smaller, but harder to solve. The iteration is repeated until either the problem is solved, or the remaining subset is too diﬃcult to solve reliably.
Phase I study of vemurafenib in children with recurrent or progressive BRAF V600E mutant brain tumors: Pacific Pediatric Consortium study (PNOC-002).

Background: BRAF V600E mutation is present in a subset of pediatric brain tumors. Vemurafenib is an oral, selective ATP-competitive inhibitor of BRAF V600E kinase. The goal of this multi-center study conducted through the Pacific Pediatric Neuro-Oncology Consortium (PNOC) was to determine the recommended phase 2 dose (RP2D) and dose limiting toxicities (DLTs) in children < 18 years with recurrent or progressive BRAF V600E mutant brain tumors. Results: Nineteen eligible patients were enrolled. Eleven patients had received three or more prior therapies. Data reported are from the start of treatment for the first patient (April 30 2014) through August 31 2019. The RP2D was defined as 550 mg/m 2 twice daily after DLT criteria adjustment for rash. Related grade ≥ 3 adverse events included secondary keratoacanthoma ( n = 1); rash ( n =16); and fever ( n = 5). Subjects received a median of 23 cycles (range 3–63). Four patients remain on treatment. Centrally reviewed best radiographic responses included 1 complete response, 5 partial responses, and 13 stable disease. The steady-state area under the curve (AUC 0-∞ median) was 604 mg*h/L (range 329–1052). Methods: Vemurafenib was given starting at 550 mg/m 2 , twice daily which corresponds to the adult RP2D. Adverse events were graded using the NIH Common Terminology Criteria for Adverse Events (CTCAE) version 4.0. Central imaging review was performed. Pharmacokinetic sampling was performed. Conclusions: Vemurafenib has promising anti-tumor activity in recurrent BRAF V600E-positive brain tumors with manageable toxicity. A phase 2 study is ongoing (NCT01748149).
Molecular Cloning and Expression of Abscisic Acid-Responsive Genes in Embryos of Dormant Wheat Seeds1

Hydrated dormant cereal seeds do not germinate even when environmental conditions are favorable for germination. By using cDNA cloning and differential screening, we have identified mRNAs from five gene families that are abundant in the embryos of imbibed, but developmentally arrested wheat (Triticum aesti- vum L.) seeds. Gene transcript levels of these mRNAs are maintained and even increase in embryos of imbibed dormant seeds for as long as the seeds remain dormant. In contrast, transcript levels decline in nondormant seeds after imbibition and disappear as germination occurs. All the identified genes are ABA responsive. Based on these data we conclude that wheat seeds in the hydrated dormant state exhibit prolonged expression of ABA-responsive genes.
The distribution of neutral gas in the Milky Way

Over the past decade, the uncertainty on the H2 mass of the inner Galaxy has been reduced from a factor ∼4 to less than a factor 2, comparable to the uncertainty which still exists for H i. Gas masses beyond the solar circle remain more uncertain, owing mainly to uncertainties in the rotation curve and CO‐to‐H2 mass conversion factor there. H i and H2 masses are roughly equal within the solar circle, but H i dominates beyond. GMCs are superior to H i clouds for tracing Galactic structure, but kinematic distance errors and severe velocity crowding near the terminal velocity in the inner Galaxy remain formidable obstacles to sorting out the structure of that region.Large‐scale near‐infrared surveys of the Galactic plane, particularly those recently obtained by the COBE satellite, hold great promise for determining both distances and masses for large GMCs in the inner Galaxy. An earlier survey by the Infrared Telescope (IRT) has already revealed a remarkably tight anticorrelation between CO and near‐infrared.
Factors influencing Tourist Satisfaction: The Moderating Role of Gastronomy and Conceptual Perspective

The study proposes the theoretical concepts to establish the moderating effect of gastronomic experience on the relationship between Service Quality, Marketing Mix, and tourist satisfaction in the Southern Thailand. The primary data will be gathered through questionnaires from 384 foreign tourists in Phuket, Surat Thani, Krabi, and Phang Nga provinces. This study will clarify the moderating effects of gastronomic experience on the relationship between Service Quality, Marketing Mix, and tourist satisfaction of Southern Thailand. The significance of this study will contribute to insights into the tourism industry with gastronomic experience and develop strategies to increase satisfaction, retention, and increased tourists to travel in Southern Thailand. The findings not only contribute to travelling operators' understanding which can create competitive advantages in terms of Service Quality, Marketing Mix and gastronomy experience in tourism in the Southern region, but contribute to the development of Social Exchange Theory (SET) and Expectation-Disconfirmation Theory (EDT) also.
Collinear and soft limits of multi-loop integrands in N = 4 Yang-Mills

: It has been argued in arXiv:1112.6432 that the planar four-point integrand in N = 4 super Yang-Mills theory is uniquely determined by dual conformal invariance together with the absence of a double pole in the integrand of the logarithm in the limit as a loop integration variable becomes collinear with an external momentum. In this paper we reformulate this condition in a simple way in terms of the amplitude itself, rather than its logarithm, and verify that it holds for two- and three-loop MHV integrands for n > 4. We investigate the extent to which this collinear constraint and a constraint on the soft behavior of integrands can be used to determine integrands. We ﬁnd an interesting complementarity whereby the soft constraint becomes stronger while the collinear constraint becomes weaker at larger n . For certain reasonable choices of basis at two and three loops the two constraints in unison appear strong enough to determine MHV integrands uniquely for all n .
DIAGNOSTIC LANGUAGE ASSESSMENT FOR L2 PRONUNCIATION: A WORKED EXAMPLE

Although pronunciation teaching has come back into fashion, pronunciation assessment remains mostly focused on evaluating global qualities (Isaacs & Trofimovich, 2017; Kang & Ginther, 2017). This assessment approach offers little to learners and teachers who desire finer-grained information about their pronunciation abilities to help guide their learning activities. Diagnostic language assessment, however, can be used to identify learner strengths and weaknesses to guide learning. While diagnostic assessment holds great promises for informing learning, there are relatively few examples of truly diagnostic tests, and this paucity is especially stark for pronunciation. This article describes the design of a diagnostic pronunciation test for Korean as a second language, which includes tasks targeting both production and perception of Korean phonemes. From this test, learners receive information on how well they perceive and produce Korean sounds, which can raise awareness of difficulties and steer learners toward suitable learning activities. In addition to describing test and task specifications, key principles for pronunciation diagnosis are reviewed. Finally, an approach to interpreting and validating diagnostic test scores is discussed.
Deﬁciency in L -Serine Deaminase Interferes with One-Carbon Metabolism and Cell Wall Synthesis in Escherichia coli K-12 (cid:1)

Escherichia coli K-12 provided with glucose and a mixture of amino acids depletes L -serine more quickly than any other amino acid even in the presence of ammonium sulfate. A mutant without three 4Fe4S L -serine deaminases (SdaA, SdaB, and TdcG) of E. coli K-12 is unable to do this. The high level of L -serine that accumulates when such a mutant is exposed to amino acid mixtures starves the cells for C 1 units and interferes with cell wall synthesis. We suggest that at high concentrations, L -serine decreases synthesis of UDP– N - acetylmuramate– L -alanine by the murC -encoded ligase, weakening the cell wall and producing misshapen cells and lysis. The inhibition by high L -serine is overcome in several ways: by a large concentration of L -alanine, by overproducing MurC together with a low concentration of L -alanine, and by overproducing FtsW, thus promoting septal assembly and also by overexpression of the glycine cleavage operon. S -Adenosylmethionine reduces lysis and allows an extensive increase in biomass without improving cell division. This suggests that E. coli has a metabolic trigger for cell division. Without that reaction, if no other inhibition occurs, other metabolic functions can continue and cells can elongate and replicate their DNA, reaching at least 180 times their usual length, but cannot divide. The Escherichia coli three genes, sdaA , sdaB , and
Functional studies of GST2, a morphogenetic transition regulator on nitrogen starvation, in Candida albicans

i CONTENTS . iii LIST OF FIGURES . vi LIST OF TABELS . viii LIST OF ABBREVIATIONS . ix INTRODUTION . 1 1. Dimorphism, an important factor of virulence . 7 2. Signaling pathways and transcriptional regulators required for morphogenesis . 8 2.1 Mitogen-activated protein kinase pathway . 8 2.2 cAMP-dependent protein kinase A pathway . 8 2.3 Ras1p-mediated signaling pathway . 10 3. Morphogenetic auto-regulatory substances . 11 4. Nitrogen starvation and morphogenesis . 12 4.1 MEP2 is required for filamentous growth of C. albicans under nitrogen starvation conditions . 12 4.2 Control of MEP2 expression by the GATA transcription factors . 13 4.3 TOR controls the GATA transcription factors . 13 5. Purpose of this study . 14 MATERIALS AND METHODS . 17 iv 1. C. albicans strains and culture conditions . 17 2. Rapid amplification of cDNA ends . 18 3. Recombinant protein construction and glutathione transferase activity assay . 18 4. Gene disruption . 19 5. DNA isolation and Southern blot analysis . 21 6. Oxidative stress sensitivity assay . 21 7. Northern hybridization . 22 8. RT-PCR and real-time quantitative PCR . 22 9. Plasmid constructions for morphogenesis . 23 10. Rapamycin sensitivity assay . 23 RESULTS . 31 1. Identification, characterization and deletion of GST2 . 31 1.1 Identification and characterization of GST2 . 31 1.2 GST2 of C. albicans has a Gst activity . 31 1.3 Deletion of GST2 increases oxidative sensitivity . 32 2. Nitrogen starvation-induced filamentous growth depends on the presence of Gst2p in C. albicans . 39 2.1 The Δgst2 mutant cells defect their filamentation under nitrogen starvation condition . 39 2.2 Nitrogen starvation induces GST2 expression . 40
Subramanian, Nachiappan and Abdulrahman, Muhammad D. and Wu, Lin and Nath, Prithwiraj (2015) Green competence framework: evidence from China

Recently Human Resources Management (HRM) functions such as recruitment, selection, training and performance evaluation are expected in considering environmental management (EM) issues. Environmental protective acts with adequate ecological knowledge and socioeconomic behavior and skills are referred to in this paper as green competencies. However, a systematic approach for developing and understanding key factors that enhance individuals’ green competencies is lacking. This study contributes to green human resource literature by integrating environmental consumer behavior literature with traditional skills and competencies literature to help firms to select the right individuals to achieve their environmental goals. Using Robert’s competencies framework and structural equation modeling (SEM), this paper empirically examines the influence of individual green competencies on organizations’ green practices and performance objectives. Our model is tested using a sample of 1,230 employees working in key industries in the Chinese coastal city of Ningbo. The results indicate that acquired green competencies are more positively associated with individuals’ green competencies and green behavior. The study empirically demonstrates that verifying acquired green competence attributes such as environmental knowledge, green purchase attitude and intention during employee selection would certainly be helpful for firms to identify individual green performance potential.
Increasing security for RC4 algorithm by using PUF-based random number generators

Rivest Cipher 4 (RC4) algorithm is the considerably stream cipher, and can be used of internet protocols. There is a quantity of weaknesses bytes even after the first 256 rounds (the size of the RC4 permutation) of the Pseudo random generation algorithm (PRGA). So far various modified RC4 research were presented but all of them have either ordinary privacy or accomplishment evaluation challenges. This paper introduces enhanced RC4 algorithm depending on Physical Unclonable Function (RC4 - PUF) which has solved both of these challenges. The principal development of the proposed RC4 - PUF methodology is that the changing of the s array reorganized to rely on the creation of a specific hardware random generator Method (HRGA) and the proposed methodology outcomes as follows: Output = Plaintext XOR generated key XOR random values from HRGA. The outcome of the tests demonstrates the refinement of the privacy of ciphers (average secrecy), randomness and accomplishment assessment (Encryption time and throughputs) over the variable key length and miscellaneous plaintext size of the proposed encryption RC4 – PUF methodology.
Projected Features for Hindi Speech Recognition System

: Automatic speech recognition (ASR) is a technology that allows a computer to identify the words uttered by a person using a microphone or telephone. The processing required is divided into two parts: the signal processing front end and statistical framework of hidden Markov model (HMM) at back-end for pattern classification. This paper presents a comparative study of different types of feature reduction techniques in the context of Hindi language. Experimental results show a significant improvement in ASR performance by using extended MF-PLP (PLP derived from Mel scale filter bank) feature extraction technique at front-end with the help of Hetroscedastic linear discriminant analysis (HLDA) projection scheme. All the investigations are based on the experiments conducted in typical field conditions using standard close talking microphone.