Miracle-dz/UniFAR-FTUs huggingface dataset:
In vivo studies on the Agrobacterium tumefaciens virulence protein VirE2 reveal self-association at the microtubules

VirE2 is an effector protein involved in Agrobacterium-Mediated Transformation (AMT) of plant cells. Here we show, using both yeast cells and plant protoplasts, that a fluorescent VirE2 fusion protein is visible as a filament between the spindle poles in dividing cells and that these filamentous structures co-localize with microtubules. Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET) visualization studies show that VirE2 self-associates and physically interacts with the Tub1 tubulin. Moreover, using a BiFC assay, we showed that VirE2 interacts with the Arabidopsis thaliana VIP1 and Agrobacterium VirE3 proteins, both possibly involved in nuclear uptake of VirE2. These interactions co-localize with the spindle pole body component Spc42. Our data suggest a role for VirE2 in guiding the T-DNA complex to the host nucleus through transport along microtubule structures and a possible role of factors located at the spindle pole bodies in nuclear uptake of VirE2. and 428-34VC[VirE2]/35VN, and by flow cytometry. Yeast cells were in MY medium supplemented with the appropriate nutrients and diluted ten-fold before flow cytometry. The Guave EasycyteTM system from MILLIPORE was used and data were analyzed with CytoSoftTM software. A 488 nm laser and a 510-540 nm band pass filter were used to detect YFP fluorescence. For each analysis 3000 cells were used.
HYBRID MEDIA APPLICATION FOR LANGUAGE STUDIES IN ELEMENTARY SCHOOL

The aim of the study was to develop a new hybrid media product for learning environments and to evaluate its suitability and potential for studying English as a foreign language in elementary school. The prototype made for the study consisted of an English book linked to a mobile phone through 2D codes. By using the code reader the user was able to carry out listening tasks and interactive grammar exercises which opened up in the mobile phone’s web browser. The hybrid book concept was evaluated by ten elementary school teachers. According to the user tests with the teachers the hybrid book would motivate pupils and allow them to study regardless of time and place. The interviewed teachers had a very positive attitude to using a mobile phone side-by-side with the text book in order to carry out tasks not possible with the text book alone (e.g. listening tasks). Most of the teachers were willing to use a hybrid book for teaching if it was available. All the interviewed teachers thought that pupils would be interested in using a hybrid book, and hence it will be interesting to observe how the pupils themselves evaluate the use of the hybrid book in the next phase of the study.
Evolution of the Oceanic Lithosphere in the equatorial Atlantic, evidence for small-scale convection from the PI-LAB experiment

The oceanic lithosphere is a primary component of the plate tectonic system, yet its evolution and its asthenospheric interaction has rarely been quantified by in-situ imaging at slow spreading systems. We use Rayleigh wave tomography from noise and teleseismic surface waves to image the shear wave velocity structure of the oceanic lithosphere-asthenosphere system from 0 to 80 My at the Equatorial Mid-Atlantic Ridge using data from the Passive Imaging of the Lithosphere-Asthenosphere Boundary (PI-LAB) experiment. We observe fast lithosphere (V SV > 4.4 km/s) that thickens from ~30 km near the ridge axis to > 50 – 80 km at seafloor > 60 My. We observe several punctuated low velocity anomalies (V SV < 4.1 km/s) in the asthenosphere between 50-150 km depth, not necessarily focused beneath the ridge axis. Some of the low velocity regions are located within 100 km of the ridge axis, but other low velocity regions are observed at distances > 400 km from the ridge. We observe a high velocity lithospheric downwelling drip beneath 30 My seafloor that extends to 80-130 km depth. The asthenospheric low velocities likely require partial melt. Although melt is present off axis, the lack of off-axis volcanism suggests the lithosphere acts as a permeability boundary for deeper melts. The punctuated and off-axis character of the asthenospheric anomalies and lithospheric drip suggest small-scale We observe a tectonic plate that generally thickens with age, but the thickening is not simple and monotonic everywhere, and there may be evidence of drips coming off the base of the plate. We also see evidence for small pockets of melt and rising mantle far away from the ridge axis. Taken together this suggests that flow in the mantle is more complicated than previously thought. Small-scale convection may occur beneath a range of seafloor ages, which may also affect the way the lithosphere cools and ages. Thus, our result has implications for how tectonic plates evolve and interact with the mantle below and how plate tectonics works to cool the earth since its formation billions of years ago.
Real‐time neural sliding mode field oriented control for a DFIG‐based wind turbine under balanced and unbalanced grid conditions

This study proposes a real-time sliding mode field oriented control for a doubly-fed induction generator (DFIG)-based wind turbine prototype connected to the grid. The proposed controller is used to track the desired direct current (DC) voltage reference at the output of the DC link, to maintain constant the grid power factor at the step-up transformer terminals controlled by the grid side converter, and to force independently the stator active and reactive power to track desired values through the rotor currents controlled by the rotor side converter. This control scheme is based on a recurrent high-order neural network (RHONN) identifier trained on-line by an extended Kalman filter. The RHONN is used to approximate the DC link and the DFIG mathematical models. The adequate approximation helps to calculate the exact equivalent control part of the sliding mode controller and to eliminate the effects of disturbances and unknown dynamics appearing in the grid, which improves the robustness of the control scheme. This controller is experimentally validated on a 1/4 HP DFIG prototype and tested for variable wind speed to track a time-varying power reference and to extract the maximum power from the wind, under both balanced and unbalanced grid conditions.
Source Term Modeling of Vortex Generators

Vortex generators (VGs) are effectively used to improve the performance of the wind turbine blades by means of flow control. VGs can be represented by local geometrical disturbance on the aerodynamic surfaces. This disturbance generates vortices in the streamwise direction, energizing the boundary layer and hence delaying or suppressing the flow separation. In this way, the entire flow field is reshaped. Use of Computational Fluid Dynamics (CFD) combined with the experiments is desirable to be able to find the most efficient geometrical shape of VG and the installation of it based on the flow characteristics of the problem. However, there is a challenge of using CFD for VG simulation which is the large number of grid points required to obtain an accurate solution. One of the ways to overcome this difficulty is to model the effect of VG. Therefore this thesis will investigate the ways to model a vortex generator and validate the capability of such a model to simulate the optimal VG shape and installation.
Vascular Responses in a Model of Sepsis Leukotrienes in Local Innate Immune versus Opposing and Hierarchical Roles of

The 5-lipoxygenase (5-LO)-derived leukotrienes (LTs) inﬂuence both local innate immunity and vascular responses, but the relative importance of effects on these two processes in sepsis is unknown. In a cecal ligation and puncture model of peritonitis with severe sepsis, 5-LO (cid:1) / (cid:1) mice showed a reduction in peritoneal neutrophil accumulation and an increase in the number of bacteria in the peritoneal cavity. Despite this impairment of local innate immunity, the null mice exhibited a marked improvement in survival, and this protection was also seen in wild-type animals treated with the LT synthesis inhibitor MK 886. A survival advantage in severe sepsis was also observed in mice treated with the cysteinyl-LT receptor antagonist MK 571, but not with the LTB 4 receptor antagonist CP 105, 696. Protection in the 5-LO (cid:1) / (cid:1) mice was associated with reduced vascular leak and serum lactate levels. Moreover, wild-type mice treated with MK 571 exhibited less sepsis-induced hypotension. These data demonstrate opposing effects of cysteinyl-LTs on innate immune vs hemodynamic responses, demonstrating protective effects on local immunity and deleterious effects on the vasculature. They also suggest the possible therapeutic utility of targeting vascular events in sepsis with cysteinyl-LT blockade. The Journal of Immunology, 2005, 174: 1616–1620.
PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF AQUEOUS AND HYDROALCOHOLIC EXTRACT OF MUSA ACUMINATA LEAVES

: This study describes the in-vitro antioxidant activity of aqueous and hydroalcoholic extract of the plant of Musa Acuminata leaves belonging to the family-Musaceace. Folin ciocalteau assaywas used to determine the total Phenolic content while aluminum chloride colorimetric method was used to determine the total flavonoid content. Some of the classical methods to estimate in-vitro antioxidant activity were the Hydrogen peroxide scavenging assay, superoxide radicals scavenging activity, nitric oxide radical scavenging assay, reducing antioxidant power assay, and hydroxyl radical scavenging activity. Less phenolic concentration was identified in aqueous extract (51.01 mg/g of extract) while more phenolic concentration was found in hydroalcoholic extract (103.4 mg/g of extract) which is similar to the case of flavonoid content. Concentration of the content played a major role in determining the reducing power activity and hydrogen peroxide scavenging assay. From the above results, the hydroalcoholic extract of Musa Acuminata shows a significant activity when compared to aqueous extract. assay, radical scavenging microsomal lipid assay.
Some Remarks on Foundation Pile Testing Procedures

This work presents the review of pile capacity testing techniques. In an overview, the key points in pile designing are: determination of the appropriate computational schemes, reliable data on loads and the properties of structural materials (in particular, of the soil mass, which is marked by the greatest variability). The procedure of constructing a pile foundation should include: carrying out soil tests in the scope that ensures safe designing, selecting a piling technology that is relevant both to geotechnical conditions and expected loads, drafting a piling design together with the design of load tests, setting up a testing station for further load tests, static and/or dynamic tests of pile load capacity, preceded by supplementary soil tests when the conditions of test pile installation fail to comply with the design assumptions or when the pile length exceeds the depth of the previously investigated soil, making documentation of load capacity tests (with an additional correction of the piling design), the actual piling (ongoing analysis of pile driving logs and, if necessary, testing the piles’ integrity), drawing up the as-built documentation. Unfortunately, the design is corrected after the load test have been conducted only if the piles fail to show the designed bearing capacity. The designer is then obliged to revise the design assumptions on the basis of tests results. If the test results account for the a greater bearing capacity than necessary and it would be recommendable to limit the extent of the planned (i.e. set out in the contract) piling works, usually neither the contractor nor the designer, nor even the Construction Site Supervisor, acting for the benefit of the Investor, are willing to take on the responsibility for reducing the scope of the piling works. The necessity of conducting additional control tests before and during the implementation of the construction project is often treated by the investors as an attempt at extorting extra financial resources or at delaying the project implementation. The designer, however, has no other possibility (and often – he/she does not have required qualifications) to verify the obtained test results.
MYST: Automated DevOps for distributed applications across heterogeneous Cloud, Fog and Edge infrastructures

The amount of data gathered by humans is increasing [1] and more and more data is being generated at the edge of the network. To lower strain in the network, we should move processing closer to the data. Moving applications closer to the edge, distributes functionality geographically, increasing complexity. Distributed applications require smarter tools that allow a developer to maintain control over their applications. We enable developers to, not only move their applications closer to the Edge but also separate them over a distributed infrastructure across the Cloud, Fog and Edge. By adding a tool in the DevOps environment, current cloud engineers can expand their applications from cloud to the fog and edge. We combine literature research with practical research, providing insights from both academia and industry. Most DevOps tools provide specific solutions for one problem and not the whole process. Current solutions[2–5] automate the process on service level infrastructure provided by cloud providers, not on the Fog and Edge. We proposed a conceptual framework, called MYST, that can automate the process of orchestrating, configuring and deploying composite applications over Cloud, Fog, and Edge devices. MYST provides control, reconfiguration and quality maintenance during the lifetime of the application. In the future, we want to implement MYST and be able to run experiments on it.
Delft University of Technology Assessing total water storage and identifying flood events over Tonlé Sap basin in Cambodia using GRACE and MODIS satellite observations combined with hydrological models

16 In this study, satellite observations including gravity (GRACE), terrestrial reflectance (MODIS), and 17 global precipitation (TRMM) data, along with the output from the PCR-GLOBWB hydrological model, 18 are used to generate monthly and sub-monthly terrestrial water storage (TWS) estimates and quantify 19 flood events over the Tonlé Sap basin between 2002 and 2014. This study is the first time GRACE data 20 have been used to investigate the hydrological processes over the Tonlé Sap basin. To improve the 21 accuracy of the TWS estimates from GRACE, a signal restoration method was applied in an effort to 22 recover the signal loss (i.e., signal leakage) inherent in the standard GRACE post-processing scheme. The 23
How to Use Qualitative Interviews in E-health Research

—Qualitative interviews are much used in e-health research. It is a challenge that qualitative studies in e-health are of varying quality, and not always based in an explicit methodological framework. In this paper, we present easy-to-use guidelines for using qualitative interviews in e-health research that are firmly based in social science methodology. The paper outlines some topics and practical advice that are of special interest for e-health. We draw on the qualitative methods literature and our own experiences from e-health research, where we have used qualitative interviews for more than 10 years and in studies among several different user groups. Qualitative interviews stand out as a well-suited method to grasp the socio-technical complexity and rapid changes that characterise the e-health sector.
Exploring Impacts of a Flipped-Instruction Mode for a Disciplinary Computer Applications Course

Dr. Hylton is an Assistant Professor of Mechanical Engineering and Coordinator of the First-Year Engineering experience for the T.J. Smull College of Engineering at Ohio Northern University. He previously completed his graduate studies in Mechanical Engineering at Purdue University, where he conducted research in both the School of Mechanical Engineering and the School of Engineering Education. Prior to Purdue, he completed his undergraduate work at the University of Tulsa, also in Mechanical Engineering. He currently teaches first-year engineering courses as well as various courses in Mechanical Engineering, primarily in the mechanics area. His pedagogical research areas include standards-based assessment and curriculum design, including the incorporation of entrepreneurial thinking into the engineering curriculum and especially as pertains to First-Year Engineering.
The Mobilisation of Muslim Women in Denmark

: Under the headline of ‘the mobilisation of Muslim women in Denmark’, this paper contains a series of introductory considerations as well as a few preliminary findings on the relatively unexplored question of how and why Muslim women in Denmark form organisations, and via their organisations interact with the surrounding society. Previous studies of immigrant mobilisation in Denmark has not paid special attention to gender as a differentiating factor, but this paper argues that there are good reasons to actually do so. Based on a series of qualitative interviews with members of various immigrant women´s organisations, this paper aims to explore patterns of networking among these women, and the level and nature of their interaction with other organisations and societal institutions.
Laser ultrasonic monitoring of the elastic properties of an apple

: Fruit firmness estimates usually require contacting or destructive methods. However, we propose to estimate firmness from an all optical, non-contacting method that generates and detects elastic waves in an apple. The compressional and shear wave velocities are governed by the fruit's elastic constants and density, which can be used to estimate firmness, non-destructively. High energy pulses of light are used to excite elastic waves via thermo-elastic expansion. Elastic waves are detected using a laser Doppler vibrometer. By exciting the apple surface at many locations we accurately estimate the compressional, shear and Rayleigh wave velocities of an apple. Repeated realizations of this experiment over 15 days to observe changes in apple elastic properties over time. Softening of the apple decreases the resonant mode frequencies and elastic constants, as well as increases wave attenuation. We find that attenuation is the most sensitive parameter to the aging of the apple. We believe that our novel method of apple interrogation could be automated, and extended to other fruits. The earliest part of the same waveforms highlight the lower-amplitude arrival of the P-waves. A linear regression on this arrival results in a P-wave speed estimate v p = 182 ± 7 m/s.
273-PRELIMINARY RESULTS OF TUNA DIET STUDIES IN THE WEST EQUATORIAL INDIAN OCEAN.

In the framework of the Thetis project, a trip on board a purse seiner was done in the West equatorial part of the Indian Ocean. 112 stomachs have been collected and analyzed. The paper presents the preliminary results of that study. The number of empty stomachs was high among the fish caught from floating objects schools. The ratio empty/filled stomachs was greater in the small size classes than in the large size. The composition of the stomach content differs according to the species and the size. Crustacean preys dominated in the diet of Skipjack caught either from free schools either from schools associated with floating object. For the individuals caught on free school, crustacean constitute the exclusive prey. Whilst its proportion decreased on FADs. These be linked to different behaviour (with emphasis on vertical migration) among the species and the size of the individuals. The fishing zone characterized by the presence of large free schools of skipjack that were feeding actively. The crustaceans were essentially represented by a small size stomatopod (Natosquilla investigatoris). The abundance of this prey among the forage might explain the low occurence of large size yellowfin and bigeye free schools in the area.
Rapid Transcriptional Regulation by Phytochrome of the Genes for Phytochrome and Chlorophyll a/b-Binding Protein in Avena sativa

We have examined phytochrome-regulated transcription of phytochrome (phy) and chlorophyll a/b binding protein (cab) genes in dark-grown Avena seedlings by using run-on transcription in isolated nuclei. Kinetic analysis of phy transcription following pulse-light treatments to produce various amounts of Pfr, the active form of phytochrome, leads to these conclusions. (i) Transcription decreases rapidly (discernible within 5 min) after Pfr formation, reaching an essentially undetectable level by 1 h. (ii) The response is very sensitive; <1% Pfr is sufficient to produce maximum feedback repression over the first 30 min. (iii) The duration of transcriptional repression is proportional to the Pfr concentration; derepression begins once the concentration fails below some saturation level because of degradation of Pfr. Concurrent analysis of cab transcription leads to these conclusions. (i) After Pfr formation, transcription increases -10-fold by 3 h, but this response is not detectable until after a 30-min lag. (ii) Detectable induction of cab requires a >30-fold-higher Pfr level than is needed to repress phy expression. (iii) Transcription returns to the preirradiation level considerably sooner than does phy transcription (<12 h versus >24 h respectively), indicating that a high level of Pfr is needed to sustain the increased transcription of cab. Taken together, these results suggest that differences in the phytochrome signal transduction pathway are responsible for the distinct patterns of regulation of these genes.