Miracle-dz/UniFAR-FTUs huggingface dataset:
C-Reactive Protein Serum Levels as an Internal Load Indicator of Sprints in Competitive Football Matches

Abstract This study compared internal load variable dynamics across three consecutive football matches and investigated its relationship with the number of sprints performed by players. Twenty-three male players had blood and salivary samples collected for hormonal concentration (testosterone, cortisol, and testosterone-cortisol ratio), and serum analysis (interleukin-6, interleukin-1-beta, and c-reactive-protein), respectively. Sprints were measured through Global Position System devices. Testosterone and testosterone-cortisol-ratio presented a decreasing behavior up to the second match, and all other indicators presented an increasing behavior during the same period, c-reactive-protein was the only indicator observed to significantly rise up to the third match as well (0.38±0.02 mg/L; 0.49±0.05 mg/L; 0.69±0.05 mg/L; 0.89±0.08 mg/L). C-reactive-protein showed strong correlations with sprints in the second and third matches (p<0.01, r=0.71 and 0.79), and weak-to-moderate in the first one (p<0.05, r=0.59). Interleukin-6 and interleukin-1-beta presented weak-to-moderate correlation in every match (p<0.05, r=0.48 to 0.51; r=0.51 to 0.55) while testosterone-cortisol ratio presented weak-to-moderate correlation only in the third one (p<0.05, r=0.42). Multilevel linear regression showed that c-reactive-protein had a higher R2 than other biomarker in any regression model (R2=0.624; p<0.001). Therefore, c-reactive-protein can be a valid and reliable indicator of sprinting in competitive football. Future research should explore longer periods of monitoring and/or others external load variables so that other behaviors may arise to knowledge.
Regarding A Systematic Review and Meta‐analysis of Predictors of Airway Intervention in Adult Epiglottitis

With great interest, I read the recent systematic review and meta-analysis of predictors of airway intervention in adult epiglottitis. I greatly appreciate Sideris et al.’s efforts, but there are several potential methodological pitfalls. Firstly, the authors claimed that the meta-analysis was performed following the guideline of Preferred Reporting Items for Systematic Reviews and MetaAnalyses Protocols, but I found that the predefined protocol was not registered in any platforms (e.g., Cochrane Library, International Prospective Register of Systematic Reviews, and BMJ Open), which may influence its transparency and reproducibility in the process and increase the risk of selective reporting bias. Secondly, the authors mentioned that only MEDLINE and Embase databases were searched in this study, but these databases seemed not to be enough to seek out all the eligible studies. Alternatively, some additional databases such as Clinical Trials Registry, Cochrane Central Register of Controlled Trials, Google Scholar, and Scopus, as well as unpublished data such as gray literature, may better be included, which could establish a more comprehensive searching of related studies. Thirdly, because the design of including studies is all retrospective, it is more appropriate to use “odds ratio (OR),” not “risk ratio (RR)” or “risk difference (RD)”, to act as the pooled-effect estimate of dichotomous variables according to the Cochrane Handbook. Actually, OR, RR, and RD are not the same. The misinterpretation would tend to overestimate/underestimate the actual effect. Therefore, OR may better be used as the summary statistic to pool data in principle. Lastly, publication bias would impact the final results of meta-analysis, so the recognition and processing of publication bias should be an indispensable step. But the authors did not evaluate publication bias. When the number of included studies is lower than 10, the Harbord test may better be routinely performed to assess the publication bias for the binary outcomes. Among these outcomes, the Harbord test and modified Galbraith plot of stridor showed that a significant level of the publication bias was identified (P = 0.003). Moreover, the heterogeneity of groups is very high and had not been explored, but the authors had not made relevant explanations. Therefore, they may better add the subgroup analyses, and the conclusion should be interpreted with caution. In summary, meta-analysis is an important method of evidence-based medicine. However, owing to the limitation of this meta-analysis, further high-quality randomized controlled trials with the larger sample size should be warranted.
Beyond supervised therapy: Promoting behavioral changes in people with MS

A critical aspect of many rehabilitation interventions for people with multiple sclerosis (MS) is incorporating strategies that support behavior change. The main purpose of this topical review was to summarize recent randomized clinical trials (RCTs) of rehabilitation interventions in which participants learn and apply skills or engage in healthy behaviors. The Capability, Opportunity, Motivation, and Behavior (COM-B) framework was used to broadly classify behavior-change strategies. The included RCTs varied widely in terms of dosing, delivery format, and types of interventionist. Commonly used behavior-change strategies include education, persuasion, and training. We recommend that researchers and clinicians use frameworks like Behavior Change Wheel and Behavior Change Technique Taxonomy to describe and classify intervention strategies used to promote behavior change. We also recommend more sophisticated RCTs be conducted (e.g. sequential multiple assignment randomized trial and three-arm RCTs) to better understand ways of promoting behavior change in rehabilitation interventions.
Mobile App Recommendation: An Involvement-Enhanced Approach

Given the ubiquitous and critical role of mobile apps in people’s lives as well as the sheer size of the market, developing effective mobile app recommendation methods that can help users locate the apps they desire is critical for both users and platforms. Premised in involvement theory, we propose a novel mobile app recommendation method that integrates both users’ download and browsing behaviors for mobile app recommendations, in contrast to existing methods that rely on download behaviors but neglect browsing behaviors. Specifically, we introduce a novel model that appropriately combines download and browsing behaviors to learn users’ overall interests in and involvement with apps, develop a new algorithm to infer the model’s parameters, and propose an innovative mobile app recommendation strategy that combines users’ overall interests and their current interests to recommend apps. Finally, using data collected from one of the largest mobile app platforms in China, we demonstrate and analyze the superior performance of our method over several state-of-the-art mobile app recommendation methods.
Using local communities to establish geographical boundaries for case studies.

BACKGROUND Case studies are widely used in the social sciences to explore complex phenomena in natural settings. Applying boundaries is vital to ensure cases are easily identifiable and similar enough to be treated as instances of the same phenomenon. AIM To discuss using local communities to establish geographical boundaries in case studies. DISCUSSION Postal codes can be used to define geographical boundaries. A broad range of statistical data can be collected from these areas, including demographic information, which can assist in identifying communities that have issues or characteristics that may benefit from further research. Collecting data from communities enables researchers to access appropriate populations. CONCLUSION Using local communities to identify case boundaries helps to provide a clear periphery to the case and generate local solutions to local problems. IMPLICATIONS FOR PRACTICE This approach enables researchers to immerse themselves in the local community, ensuring it engages with research projects and findings.
Structural and functional characterization of dynamic oligomerization in Burkholderia cenocepacia HMG-CoA reductase.

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (HMGR), in most organisms, catalyzes the four-electron reduction of the thioester (S)-HMG-CoA to the primary alcohol (R)-mevalonate, utilizing NADPH as the hydride donor. In some organisms, including the opportunistic lung pathogen Burkholderia cenocepacia, it catalyzes the reverse reaction, utilizing NAD+ as a hydride acceptor in the oxidation of mevalonate. Burkholderia cenocepacia HMGR has been previously shown to exist as an ensemble of multiple non-additive oligomeric states, each with different levels of enzymatic activity, suggesting that the enzyme exhibits characteristics of the morpheein model of allostery. We have characterized a number of factors, including pH, substrate concentration and enzyme concentration, that modulate the structural transitions that influence the transition between the multiple oligomeric states. We have also determined the crystal structure of Burkholderia cenocepacia HMGR in the hexameric state bound to coenzyme A and ADP. This hexameric state provides important clues as to how the transition between oligomeric states might occur, and why Burkholderia cenocepacia HMGR, unique among characterized HMGRs, exhibits morpheein-like behavior.
A Model for E-commerce Market Network with Improved Evolution Mechanism

In order to investigate the formation of e-commerce market network, this paper describes an analytical framework from a complex network point of view which contains three steps—definition of network, analysis of network topology and analysis of network environment. Then, an innovative model for explaining the evolutionary process is proposed, with several original factors—growth-factor, select-order-factor, preferential attachment mechanism and global-local-factor. Our research reveals that the attraction mechanism impacts evolutionary trend and network structure to some extent, and also reveals that the global-local-factor and select-order-factor impact the evolutionary structure of the network, the smaller the probability, the smaller the concentration of networks and the more obvious the randomness are. In order to analyze the impact of edge-increasing mechanism on network evolutionary trend, a contrast test is designed with two models. The test reveals that the edge-increasing mechanism makes the network become a small world with a lower average distance and a higher clustering coefficient, and makes it be like a scale-free network with a lower power-law exponent and a higher centralization.
Control of homologous recombination by the HROB–MCM8–MCM9 pathway

In this study, Hustedt et al. use CRISPR-based genetic screens to build a clear picture of the postsynaptic steps of homologous recombination in mammalian cells. They report the identification of C17orf53/HROB, a factor required for cell survival after exposure to a variety of replication stress-inducing genotoxins and for the resolution but not formation of Rad51 foci.
Rethinking the Future of Food Packaging: Biobased Edible Films for Powdered Food and Drinks

In today’s society, packaging is essential. Without this, the materials would be messy and ineffective. Despite the importance and key role of packaging, they are considered to be useless, as consumers see it as a waste of resources and an environmental threat. Biopolymer-based edible packaging is one of the most promising solutions to these problems. Thus, inulin, biopolymers such as agar and sodium alginate, and glycerol were used to develop a single use edible material for food packaging. These biofilms were obtained and tested for three months. For inulin-based films, the results highlight improvements not only in physical properties (homogeneity, well-defined margins, light sweet taste, good optical properties, high solubility capacity or, as in the case of some samples, complete solubilization), but also superior mechanical properties (samples with high inulin content into composition had high tensile strength and extremely high elongation values). Even after three months of developing, the values of mechanical properties indicate a strong material. The optimization establishes the composition necessary to obtain a strong and completely water-soluble material. This type of packaging represents a successful alternative for the future of food packaging: they are completely edible, biodegradable, compostable, obtained from renewable resources, and produce zero waste, at low cost.
Glycolipids Recognized by A2B5 Antibody Promote Proliferation, Migration, and Clonogenicity in Glioblastoma Cells

A2B5+ cells isolated from human glioblastomas exhibit cancer stem cell properties. The A2B5 epitope belongs to the sialoganglioside family and is synthetized by the ST8 alpha-N-acetyl-neuraminidase α-2,8-sialyltransferase 3 (ST8SIA3) enzyme. Glycolipids represent attractive targets for solid tumors; therefore, the aim of this study was to decipher A2B5 function in glioblastomas. To this end, we developed cell lines expressing various levels of A2B5 either by genetically manipulating ST8SIA3 or by using neuraminidase. The overexpression of ST8SIA3 in low-A2B5-expressing cells resulted in a dramatic increase of A2B5 immunoreactivity. ST8SIA3 overexpression increased cell proliferation, migration, and clonogenicity in vitro and tumor growth when cells were intracranially grafted. Conversely, lentiviral ST8SIA3 inactivation in low-A2B5-expressing cells resulted in reduced proliferation, migration, and clonogenicity in vitro and extended mouse survival. Furthermore, in the shST8SIA3 cells, we found an active apoptotic phenotype. In high-A2B5-expressing cancer stem cells, lentiviral delivery of shST8SIA3 stopped cell growth. Neuraminidase treatment, which modifies the A2B5 epitope, impaired cell survival, proliferation, self-renewal, and migration. Our findings prove the crucial role of the A2B5 epitope in the promotion of proliferation, migration, clonogenicity, and tumorigenesis, pointing at A2B5 as an attractive therapeutic target for glioblastomas.
Variations in implementation of bladder training for overactive bladder: an online international survey

Abstract We distributed an online questionnaire to 365 health care professionals specialising in urogynaecology, using the International Urogynaecology Association (IUGA) and British Society of Urogynecolgoy (BSUG) directories to investigate how the bladder training (BT) is provided in their units. We assessed the techniques provided, who provides them, and how these techniques are implemented and taught to the patients. Two hundred and thirteen (59%) completed responses have been received. 88% of the respondents felt that BT is an important and effective tool in treating overactive bladder. 50.7% indicated those physiotherapists are the health care practitioners responsible to deliver BT in their units. 88.3% of respondents use face-to-face consultation to deliver BT, 11% only use leaflets sent to patients. 87% use bladder diaries before implementing their BT. 26.4% use anti-muscarinic medications with BT from the start. IMPACT STATEMENT What is already known on this subject? Bladder training (BT) is a well-established behavioural therapy used to treat overactive bladder with comparable efficacy to pharmacotherapy with less side effects. It I recommended to be offered as first line therapy for at least 6 weeks. However, there is no clarity about the techniques and the methods used to deliver it. What do the results of the study add? The current study aimed to find out how the BT programme delivered to patients using an online questionnaire directed to a mixed group of medical urogynecology practitioners in different geographical regions. It showed that there is a wide variation in how the BT is delivered and lack of consistency in the techniques used and the duration recommended. What are the implications of these findings for clinical practice and/or further research? Future well-designed research is needed to look at the best ways to give BT instructions, the frequency of supervision of patients who use BT, the appropriate time length of the BT programme, and the best strategies to control urgency.
Emergence and Dissemination of mcr-Carrying Clinically Relevant Salmonella Typhimurium Monophasic Clone ST34

Antibiotic resistance in bacteria is one of the urgent threats to both public and global health. The Salmonella Typhimurium monophasic sequence type 34 (ST34) clone, with its rapid dissemination and resistance to numerous critical antimicrobials, has raised global concerns. Here, we present an updated overview on the emerging infections caused by mobile colistin resistance (mcr)-carrying colistin-resistant ST34 isolates, covering their global dissemination and virulence-associated efficacy. The higher rates of mcr-1-positive ST34 in children in China highlights the increasing threat caused by this pathogen. Most of the ST34 isolates carrying the mcr-1 gene were isolated from animals and food products, indicating the role of foodborne transmission of mcr-1. The emergence of multidrug resistance genes along with various virulence factors and many heavy metal resistance genes on the chromosome and plasmid from ST34 isolates will challenge available therapeutic options. The presence of the colistin resistance gene (mcr-1, mcr-3, and mcr-5) with the multidrug-resistant phenotype in ST34 has spread across different countries, and most of the mcr-1 genes in ST34 isolates were detected in plasmid type IncHI2 followed by IncI2, and IncX4. Together, mcr-carrying S. Typhimurium ST34 may become a new pandemic clone. The fast detection and active surveillance in community, hospital, animal herds, food products and environment are urgently warranted.
The combined risk effect among BIN1, CLU, and APOE genes in Alzheimer’s disease

Abstract Genome-wide associations studies (GWAS) are detecting new variants associated with late-onset of Alzheimer’s disease (LOAD), a multifactorial neurodegenerative disorder. The variants rs744373 BIN1, rs11136000 CLU and rs3764650 ABCA7 uncovered by GWAS led to different AD pathways, such as metabolism, trafficking and endocytosis of lipids and inflammation. However, most of the association studies did not replicate these variants with significance. This could be due to a small power effect evident when these variants are tested independently with LOAD. Therefore, we aimed to investigate whether the combination of different variants would additively modify the risk of association with LOAD that is observed in GWAS. We performed an association study testing pairwise variants in metabolism, trafficking and endocytosis of lipid (rs429358 and rs7412 APOE, rs744373 BIN1, rs3764650 ABCA7 and rs11136000 CLU) pathways with LOAD in samples from southeastern Brazil. Our data suggest a risk effect for LOAD between APOE with CLU and APOE with BIN1 genes.
Gene Expression Maps in Plants: Current State and Prospects

For many years, progress in the identification of gene functions has been based on classical genetic approaches. However, considerable recent omics developments have brought to the fore indirect but high-resolution methods of gene function identification such as transcriptomics, proteomics, and metabolomics. A transcriptome map is a powerful source of functional information and the result of the genome-wide expression analysis of a broad sampling of tissues and/or organs from different developmental stages and/or environmental conditions. In plant science, the application of transcriptome maps extends from the inference of gene regulatory networks to evolutionary studies. However, only some of these data have been integrated into databases, thus enabling analyses to be conducted without raw data; without this integration, extensive data preprocessing is required, which limits data usability. In this review, we summarize the state of plant transcriptome maps, analyze the problems associated with the combined analysis of large-scale data from various studies, and outline possible solutions to these problems.
Internet‐based pharmacy and centralised dispensing: an exploratory mixed‐methods study of the views of family practice staff

Over the last decade, technological advances, market competition and increasing pressures for efficiencies across healthcare systems have resulted in changes to the processes and policies involved in medicines prescribing and dispensing. The aim of this study was to explore the views of family practice staff, including GPs, on the perceived impact of changes associated with remote dispensing and the increasing availability of distance‐selling pharmacies.
Ibrutinib monotherapy outside of clinical trial setting in Waldenström macroglobulinaemia: practice patterns, toxicities and outcomes

Ibrutinib‐related data in Waldenström macroglobulinaemia (WM) remain sparse, particularly outside of trials. We report on 80 patients [previously treated, n = 67 (84%), treatment‐naïve, n = 13 (16%)] with WM, evaluated consecutively at Mayo Clinic, who received ibrutinib off‐study after its approval in 2015 for WM. Overall response rate (ORR) was 91%; major‐response rate (MRR) was 78%. The median time to first response and best response was 2·9 [95% confidence interval (CI): 2–4] and 5·7 (95% CI: 4–12) months, respectively. The median follow‐up was 19 (95% CI: 14–21) months; 18‐month progression‐free survival (PFS) was 82%. The median time on therapy was 12·5 (95% CI: 9·3–16·7) months, and the median duration‐of‐response was 32 (range: 23–32) months. Twenty‐five patients (31%) had discontinued therapy at last follow‐up (68% due to treatment‐related toxicities) and 18% of patients required dose reduction. Fatigue (12%) and atrial‐fibrillation (11%) were common non‐haematological toxicities. IgM rebound occurred in 36% of patients who abruptly discontinued ibrutinib. Following ibrutinib discontinuation, 84% of patients received subsequent treatment, achieving an ORR of 57% and MRR of 50%. The median PFS from commencement of subsequent salvage therapy was 18 months. Ibrutinib therapy, outside of clinical trials, is effective in WM, but is associated with toxicities and challenges, including IgM rebound and a high drug discontinuation rate for reasons other than disease progression.
High Performance of Linear Permanent Magnet Synchronous Motor Drive using Unscented Kalman Filter

Precise operation of Permanent Magnet Linear Synchronous Motor (LPMSM) requires information of speed and position of the motor which is generally sensed by a linear encoder. The cost of linear encoder is about one third of the motor. Installation and maintenance of linear encoders are problematic in many cases. To overcome this problem and to reduce the cost of drive, sensor less control of LPMSM drive is proposed in this work. Different estimation methods are in vogue for determination of the mover position and speed from the knowledge of the motor current and voltage. Extended Kalman Filter (EKF) is one amongst them. In this method, the system matrix is linearised up to first order Taylor expansion, and this causes errors owing to truncation of higher order terms. To minimize the estimation error, Unscented Kalman Filter (UKF) is implemented in this paper. Observations confirm superior performance of UKF over EKF in tracking the speed during transient conditions of sudden changes in load and speed. Simulation is performed extensively using Simulink / embedded MATLAB and is validated experimentally using DS1104 controller board.