Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytokinesis and postabscission midbody remnants are regulated during mammalian brain development

Significance During prenatal brain development, neural stem cells must divide to produce billions of daughter cells. At early stages, they produce more stem cells. Later, they produce neurons. Errors in their divisions can result in abnormal brains. We address whether cytokinesis—cleavage furrowing and intercellular bridge severing (abscission)—occurs differently at different ages and in a small-brained mouse mutant of a kinesin motor protein gene. We developed a method to image neural stem cells undergoing cytokinesis in brain explants. We found that remnants of severed intercellular bridges are more likely to remain on daughter cells at earlier ages. In the kinesin gene mutant, bridge severing occurs faster. These findings suggest that precise regulations of cytokinesis are important during early brain development. Building a brain of the proper size and structure requires neural stem cells (NSCs) to divide with tight temporal and spatial control to produce different daughter cell types in proper numbers and sequence. Mammalian NSCs in the embryonic cortex must maintain their polarized epithelial structure as they undergo both early proliferative divisions and later neurogenic divisions. To do this, they undergo a polarized form of cytokinesis at the apical membrane that is not well understood. Here, we investigate whether polarized furrowing and abscission in mouse NSCs are regulated differently at earlier and later stages and in a cytokinesis mutant, Kif20b. This mutant was previously shown to have microcephaly and elevated apoptosis of NSCs. We developed methods to live image furrow ingression and midbody abscission in NSCs within cortical explants. We find that polarized furrow ingression occurs at a steady rate and completes in ∼15 min at two different ages. However, ingression is slower in a subset of Kif20b mutant NSCs. Abscission is usually observed on both sides of the midbody and takes 65 to 75 min to complete. Surprisingly, abscission is accelerated in the Kif20b mutant NSCs. Postabscission midbody remnants are observed at the apical membranes of daughter cells and are much more abundant in early-stage cortices. After NSC divisions in vitro, midbody remnants are more often retained on the daughter cells of early proliferative divisions. Altogether, these results suggest that regulation of abscission timing and midbody remnants in embryonic NSCs may influence proper brain growth and structure.
Loci associated with conception rate in crossbred beef heifers

The inability of beef cattle to maintain full term pregnancies has become an economic concern for the beef industry. Herd management and nutritional improvements have alleviated environmental impacts on embryonic and fetal loss, yet additional gains can be made through genomic selection. The objectives of this study were to identify loci and gene-sets in crossbred beef heifers associated with the number of services required to become pregnant (TBRD) and heifer conception rate at first service (HCR1). Heifers (n = 709) from a commercial beef operation underwent one round of artificial insemination, before exposure to bulls for natural service for 50 days. Pregnancy and time of conception was determined by ultrasound 35 days after the breeding season. Heifers were genotyped using the GeneSeek (Lincoln, NE) Bovine GGP50K BeadChip prior to genome-wide association analyses (GWAA) conducted using an EIGENSTRAT-like model to identify loci associated (P < 1 × 10−5) with TBRD and HCR1. One locus was associated (P = 8.97 × 10−6) with TBRD on BTA19 and included the positional candidate gene ASIC2, which is differentially expressed in the endometrium of fertility classified heifers, and the positional candidate gene, SPACA3. Gene-set enrichment analyses using SNP (GSEA-SNP) data, was performed and identified one gene-set, oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen as enriched (NES = 3.15) with TBRD and contained nine leading edge genes that contributed to the enrichment of the gene set. The enriched gene-set is involved in catalyzing oxidation-reduction reactions, which have been associated with oxidative stressors impacting pregnancy success. No loci were associated nor gene-sets enriched with HCR1. Identification of loci, positional candidate genes, gene-sets and leading edge genes enriched for fertility facilitate genomic selection that allows producers to select for reproductively superior cattle, reduce costs associated with infertility, and increase percent calf crop.
Near-Complete Genome Sequences of New Strains of Nylanderia Fulva Virus 1 from Solenopsis invicta

Nylanderia fulva virus 1 (NfV-1) is a single-stranded positive-sense RNA virus that infects the tawny crazy ant. Three near-complete genomes of NfV-1SI (92% to 94% nucleotide identity to reference strain NfV-1) found infecting the red imported fire ant were determined. The genomes have 10,904 to 10,918 nucleotides and include most of the coding region for the polyprotein. ABSTRACT Nylanderia fulva virus 1 (NfV-1) is a single-stranded positive-sense RNA virus that infects the tawny crazy ant. Three near-complete genomes of NfV-1SI (92% to 94% nucleotide identity to reference strain NfV-1) found infecting the red imported fire ant were determined. The genomes have 10,904 to 10,918 nucleotides and include most of the coding region for the polyprotein.
[Effect of herb-partitioned moxibustion at fanwei point on plasma motilin and serum gastrin in patients of diabetic gastroparesis].

OBJECTIVE To explore the clinical therapeutic effect of herb-partitioned moxibustion at fanwei point in patients of diabetic gastroparesis differentiated as spleen and stomach deficiency and retention of turbid dampness as well as its effect mechanism. METHODS A total of 134 patients with diabetic gastroparesis were randomized into an observation group and a control group, 67 cases in each one. In the observation group, herb-partitioned moxibustion at fanwei point was adopted, 40 min each time, once a day for 5 times a week. In the control group, itopride hydrochloride tablets were prescribed for oral administration, 50 mg each time, three times a day. A total of 6 weeks of treatment was required in the two groups. Before and after treatment, the gastroparesis cardinal symptom index (GCSI) scores, 4-hour gastric emptying rate, TCM symptom score, as well as the levels of plasma motilin and serum gastrin were observed in the patients of the two groups. Additionally, the clinical therapeutic effect was evaluated in the two groups. RESULTS After treatment, the score of every item of GCSI, TCM symptom scores and the levels of plasma motilin and serum gastrin were all reduced as compared with those before treatment in the patients of the two groups (P<0.05), and those in the observation group were lower than the control group (P<0.05). Regarding 4-hour gastric emptying rates, which were increased as compared with those before treatment in the two group (P<0.05), and the rate in the observation group was higher remarkably than that in the control group (P<0.05). The total effective rate was 92.5% (62/67) in the observation group, higher than 74.6% (50/67) in the control group (P<0.05). CONCLUSION Herb-partitioned moxibustion at fanwei point relieves the clinical symptoms in the patients with diabetic gastroparesis and increases the gastric emptying rate, which is probably related to the regulation of the levels of plasma motilin and serum gastrin.
Changes in climate and land-use interact to create an ecological trap in a migratory species.

Anthropogenic landscape alteration and climate change can have multi-scale and inter-related effects on ecological systems. Such changes to the environment can disrupt the connection between habitat quality and the cues that species use to identify quality habitat, which can result in an ecological trap. Ecological traps are typically difficult to identify without fine-scale information on individual survival and fitness, but this information is rarely available over large temporal and spatial scales. The Prairie Pothole Region (PPR) of the United States and Canada has undergone extensive changes in the latter half of the 20th century due to advancements in agricultural technologies, water management practices, and climate change. Historically, the PPR has been a highly productive area for breeding waterfowl. While the overall trends for dabbling ducks in the PPR have exhibited increasing abundances since the late 1980s, some species, such as the northern pintail, have been declining in abundance. We used a long-term data set of pintail counts across the PPR to separate count data into a demographic process and a habitat selection process using a hierarchical model. The hierarchical model provided an alternative way of identifying ecological traps in the absence of individual survival and fitness. Our model also allowed us to account for the indirect pathways by which climate and agriculture impact pintail through their additional contribution to wetland availability, which is a primary driver of pintail demography and habitat selection. Decoupling these processes allowed us to identify an ecological trap related to increasing cropland land cover, in which pintail selected for cropland over alternative nesting habitat, likely due to the similarities with productive native mixed-grass prairie. However, large proportions of cropland within a region resulted in fewer pintail the following year, likely due to nest failures from predation and agricultural practices. In addition, we identified several regions in Canada where this ecological trap is contributing significantly to mismatches between habitat selection and demographic processes.
An Energy Efficient and Secure IoT-Based WSN Framework: An Application to Smart Agriculture

Wireless sensor networks (WSNs) have demonstrated research and developmental interests in numerous fields, like communication, agriculture, industry, smart health, monitoring, and surveillance. In the area of agriculture production, IoT-based WSN has been used to observe the yields condition and automate agriculture precision using various sensors. These sensors are deployed in the agricultural environment to improve production yields through intelligent farming decisions and obtain information regarding crops, plants, temperature measurement, humidity, and irrigation systems. However, sensors have limited resources concerning processing, energy, transmitting, and memory capabilities that can negatively impact agriculture production. Besides efficiency, the protection and security of these IoT-based agricultural sensors are also important from malicious adversaries. In this article, we proposed an IoT-based WSN framework as an application to smart agriculture comprising different design levels. Firstly, agricultural sensors capture relevant data and determine a set of cluster heads based on multi-criteria decision function. Additionally, the strength of the signals on the transmission links is measured while using signal to noise ratio (SNR) to achieve consistent and efficient data transmissions. Secondly, security is provided for data transmission from agricultural sensors towards base stations (BS) while using the recurrence of the linear congruential generator. The simulated results proved that the proposed framework significantly enhanced the communication performance as an average of 13.5% in the network throughput, 38.5% in the packets drop ratio, 13.5% in the network latency, 16% in the energy consumption, and 26% in the routing overheads for smart agriculture, as compared to other solutions.
Astigmatism prediction in small-incision lenticule extraction.

PURPOSE To investigate whether postoperative-induced refractive astigmatism after small-incision lenticule extraction (SMILE) could be predicted by preoperative objective astigmatism measured with autorefraction, keratometry, and Scheimpflug tomography. SETTING University eye clinic. DESIGN Retrospective case series. METHODS Only eyes without preoperative subjective astigmatism treated with SMILE for myopia were included. Postoperative subjective astigmatism was compared with preoperative objective astigmatism. Examinations were performed before SMILE and 3 months postoperatively and included subjective refraction, keratometry, autorefraction, and Scheimpflug tomographer measurements. Astigmatism was analyzed using double-angle plots and multivariate statistics. RESULTS A total of 358 eyes of 358 patients were included. The mean preoperative sphere was -7.33 diopter (D) ± 1.46 (SD). The postoperative spherical equivalent was -0.30 ± 0.49 D. Postoperatively, 79.6% and 98.9% of patients had a subjective cylinder ≤0.50 D and ≤1.00 D, respectively. Preoperative objective astigmatism measured with keratometry, autorefraction, and Scheimpflug tomography was significantly different (P < .05) from postoperative subjective refraction when all patients were analyzed; for patients with postoperative refractive astigmatism ≥0.50 D, preoperative astigmatism with keratometry and Scheimpflug tomography was not significantly different from postoperative refractive astigmatism. Preoperative objective astigmatism ≥0.50 D increased the risk ratio of postoperative subjective astigmatism ≥0.50 D by 2.2 (P < .001). CONCLUSIONS Preoperative objective astigmatism could not be directly interchanged with postoperative subjective astigmatism, but the presence of preoperative astigmatism ≥0.50 D doubled the risk of inducing a postoperative subjective astigmatism ≥0.50 D. Extra care when performing subjective refraction should be taken in the presence of high objective astigmatism.
Obfuscation of Malicious Behaviors for Thwarting Masquerade Detection Systems Based on Locality Features

In recent years, dynamic user verification has become one of the basic pillars for insider threat detection. From these threats, the research presented in this paper focuses on masquerader attacks, a category of insiders characterized by being intentionally conducted by persons outside the organization that somehow were able to impersonate legitimate users. Consequently, it is assumed that masqueraders are unaware of the protected environment within the targeted organization, so it is expected that they move in a more erratic manner than legitimate users along the compromised systems. This feature makes them susceptible to being discovered by dynamic user verification methods based on user profiling and anomaly-based intrusion detection. However, these approaches are susceptible to evasion through the imitation of the normal legitimate usage of the protected system (mimicry), which is being widely exploited by intruders. In order to contribute to their understanding, as well as anticipating their evolution, the conducted research focuses on the study of mimicry from the standpoint of an uncharted terrain: the masquerade detection based on analyzing locality traits. With this purpose, the problem is widely stated, and a pair of novel obfuscation methods are introduced: locality-based mimicry by action pruning and locality-based mimicry by noise generation. Their modus operandi, effectiveness, and impact are evaluated by a collection of well-known classifiers typically implemented for masquerade detection. The simplicity and effectiveness demonstrated suggest that they entail attack vectors that should be taken into consideration for the proper hardening of real organizations.
Good performance of p16/Ki‐67 dual‐stain cytology for detection and post‐treatment surveillance of high‐grade CIN/VAIN in a prospective, cross‐sectional study

The limited sensitivity of Papanicolaou (Pap) cytology and the low specificity of HPV testing in detecting cervical or vaginal lesions means that either precancers are missed or women without lesions are overtreated. To improve performance outcomes, p16/Ki‐67 dual‐stain cytology has been introduced as a useful biomarker.
Students Living in the Islands are Heavier and have Lower Fitness Levels Compared to their Mainland Counterparts; Results from the National Action for Children’s Health (EYZHN) Program

Abstract Few studies have examined variations in obesity by geographic location in youth and its relation with fitness levels. The present study investigated the association between geographic status (islands versus mainland), excess of body weight and fitness levels among 335.810 schoolchildren (male: 51.3%, 6–18 y, during the school year 2014–2015). Students’ anthropometric parameters and fitness levels - accessed via the Euro-fit test - were measured by trained physical education teachers and evaluated according to published norms. Prevalence of overweight (23.0 Vs 21.8%) and obese (10.1 Vs 8.0%) was significantly higher for students living in the islands contrary to their mainland counterparts. A significant difference was also observed for centrally obese children (33.5 Vs 28.2%). Except for speed test . 408), in all other four fitness tests, the students from the islands presented significantly lower performance (≤25th percentile of published age- and sex-specific normative values) versus their mainland counterparts. Boys and girls living in the islands had 48% and 37% increased odds of low physical fitness (as a total), respectively, compared to their mainland counterparts. Likewise, children living in islands presented increased odds of being overweight or obese by 19% and 15% in boys and girls, respectively, as compared to those living in the mainland. Increased general and abdominal adiposity have a direct negative impact on students’ performance in Physical Fitness tests. Our data highlight the problem of excessive body weight that children living in rural areas, face.
Joint Resource Optimization for Orthogonal Frequency Division Multiplexing Based Cognitive Amplify and Forward Relaying Networks

This paper investigates two resource allocation problems in cognitive relaying networks where both secondary network and primary network coexist in the same frequency band and adopt orthogonal frequency division multiplexing (OFDM) technology. The first one is the sum rate maximization problem of a secondary network under total power budget of a secondary network and tolerable interference constraint of a primary network. The second one is the sum rate maximization problem of a secondary network under separate power budgets of a secondary network and tolerable interference constraint of a primary network. These two optimization problems are completely different from those in traditional cooperative communication due to interference management constraint condition. A joint optimization algorithm is proposed, where power allocation and subcarrier pairing are decomposed into two subproblems with reasonable cost. The first one is a closed form solution of power allocation of the secondary network while managing the interference to a primary network under a constraint condition. The other is optimal subcarrier pairing at given power allocation. Simulation results reveal aspects of average signal to noise ratio (SNR), interference level, relay position, and power ratio on the sum rate of a secondary network.
Variability of cost trajectories over the last year of life in patients with advanced breast cancer in the Netherlands

Objective In breast cancer patients, treatment at the end of life accounts for a major share of medical spending. However, little is known about the variability of cost trajectories between patients. This study aims to identify underlying latent groups of advanced breast cancer patients with similar cost trajectories over the last year before death. Methods Data from deceased advanced breast cancer patients, diagnosed between 2010 and 2017, were retrieved from the Southeast Netherlands Advanced Breast Cancer (SONABRE) Registry. Costs of hospital care over the last twelve months before death were analyzed, and the variability of longitudinal patterns between patients were explored using group-based trajectory modeling. Descriptive statistics and multinomial logistic regression were applied to investigate differences between the identified latent groups. Results We included 558 patients. Over the last twelve months before death, mean hospital costs were €2,255 (SD = €492) per month. Costs increased over the last five months and reached a maximum of €3,614 in the last month of life, driven by hospital admissions, while spending for medication declined over the last three months of life. Based on patients’ individual cost trajectories, we identified six latent groups with distinct longitudinal patterns, of which only two showed a marked increase in costs over the last twelve months before death. Latent groups were constituted of heterogeneous patients, and clinical characteristics explained membership only to a limited extent. Conclusions The average costs of advanced breast cancer patients increased towards the end of life. However, we uncovered several latent groups of patients with divergent cost trajectories, which did not reflect the overall increasing trend. The mechanisms underlying the variability in cost trajectories warrants further research.
Distress Due to Urinary Problems and Psychosocial Correlates among Retired Men in Hong Kong

Urinary problems are common among aging men, but there is a paucity of research efforts to understand the psychosocial aspects of the illness. This study aims to understand how common and distressing urinary problems are for newly retired men in Hong Kong and to test the associations between mental health, self-stigma of seeking help, fatigue, self-efficacy, self-esteem, and distress due to urinary problems. To assess this, 139 out of 200 members of a retired men’s social club (mean age 63.5) were successfully interviewed. Two-fifths of the participants felt distressed due to their urinary problems and one-third of the participants had been troubled by urinary incontinence or nocturia in the past six months. Yet the majority of the participants (55%) did not seek help from any medical profession. The group who were distressed by urinary problems showed significantly poorer mental health, reported more fatigue symptoms, were less satisfied with their sexual relationships and overall self-esteem, and were less able to stop unpleasant thoughts or to get social support than the non-distressed group. Cultural perceptions of masculinity and decreased sexual vigor might have affected participants’ willingness to seek help at an early stage. Targeted health education, mutual support groups, and sensitively designed services at the community level are suggested to address these physical and mental health issues.
Oxygen K-edge X-ray Absorption Spectra

We review oxygen K-edge X-ray absorption spectra of both molecules and solids. We start with an overview of the main experimental aspects of oxygen K-edge X-ray absorption measurements including X-ray sources, monochromators, and detection schemes. Many recent oxygen K-edge studies combine X-ray absorption with time and spatially resolved measurements and/or operando conditions. The main theoretical and conceptual approximations for the simulation of oxygen K-edges are discussed in the Theory section. We subsequently discuss oxygen atoms and ions, binary molecules, water, and larger molecules containing oxygen, including biomolecular systems. The largest part of the review deals with the experimental results for solid oxides, starting from s- and p-electron oxides. Examples of theoretical simulations for these oxides are introduced in order to show how accurate a DFT description can be in the case of s and p electron overlap. We discuss the general analysis of the 3d transition metal oxides including discussions of the crystal field effect and the effects and trends in oxidation state and covalency. In addition to the general concepts, we give a systematic overview of the oxygen K-edges element by element, for the s-, p-, d-, and f-electron systems.