Miracle-dz/UniFAR-FTUs huggingface dataset:
Androgen binding in nuclear matrix of human genital skin fibroblasts from patients with androgen insensitivity syndrome.

Specific sex steroid-binding sites are associated with the salt-insoluble nuclear matrix from which lipids, histones, and chromatin have been extracted. In intact cultured normal human genital skin fibroblasts incubated for 1 h at 37 C with a saturating concentration (2 nM) of [3H]dihydrotestosterone [( 3H]DHT), approximately 50% of the total intracellular androgen receptor-steroid complexes were found in the nucleus. Within isolated nuclei from such cells, 28-49% of the specific androgen receptor binding was associated with the nuclear matrix. The antiandrogen cyproterone acetate inhibited DHT binding within the nuclear matrix. Cultured genital skin fibroblasts from two unrelated patients with receptor-positive complete androgen insensitivity (CAIS, AR+), had normal (approximately 50%) nuclear binding of DHT, and 35% and 45% of it was localized to the nuclear matrix. Genital skin fibroblasts from a patient with receptor-negative complete androgen insensitivity (CAIS, AR-) had no specific DHT binding in isolated nuclei or nuclear matrix. Scatchard analysis of specific DHT binding in the nuclear matrix isolated from cells of normal subjects after an in vitro exchange assay (0 C; 24 h) revealed the presence of saturable (maximum binding, approximately equal to 200 fmol/mg nuclear DNA), high affinity (Kd approximately equal to 1.0 nM) binding sites. By contrast, in the nuclear matrix isolated from cells of a patient with CAIS, AR+, the binding affinity for DHT was 3-fold lower (Kd approximately equal to 3.0 nM). When cytosolic androgen receptor-DHT complexes prepared from cells preincubated at 37 C for 1 h with [3H]DHT were incubated at 0 C for 1 h with isolated nuclei and nuclear matrix in the presence of 0.15 M KCl, 40-60% of specific nuclear binding was associated with the nuclear matrix. In these cell-free in vitro experiments, radiolabeled DHT-receptor complexes prepared from normal or mutant cells were mixed with isolated nuclei and nuclear matrix prepared from cells of normal subjects or patients with CAIS, AR+ or CAIS, AR-. Under these conditions, specific DHT binding in nuclei and nuclear matrix was quantitatively similar in the presence of a mutant (CAIS, AR+) receptor-steroid complex or in the presence of nuclei or nuclear matrix from the mutant cells (CAIS, AR- or AR+) when compared simultaneously with the same subcellular fractions prepared from the cells of normal subjects. (ABSTRACT TRUNCATED AT 400 WORDS)
Institute of Medicine report on prevention of mental disorders. Summary and commentary.

A comprehensive report mandated by the U.S. Congress on the state of the science of prevention recommends a stricter definition of the term prevention; summarizes specific preventive intervention research programs across the life span; and specifies funding, personnel, and coordination priorities to build a national prevention research infrastructure. A major conceptual recommendation is a focus on reducing risk for mental disorders, which has been a fruitful strategy in the prevention of physical illness. The report argues that the prevention field should also draw from advances in mental disorder treatment research and from the major contributions to the knowledge base occurring in the behavioral and biological core sciences. Relevant advances in neuroscience, genetics, epidemiology, and developmental psychopathology are examined in detail. Five major disorders are chosen to illustrate possible approaches to prevention: conduct disorder, depressive disorders, alcohol abuse and dependence, schizophrenia, and Alzheimer's disease.
Effects of a new modified, balanced hydroxyethyl starch preparation (Hextend) on measures of coagulation.

BACKGROUND Hydroxyethyl starch (HES) may affect blood coagulation. We studied the effects of a modified, balanced, high-molecular weight [mean molecular weight (MW) 550 kDa], high-substituted [degree of substitution (DS) 0.7] HES preparation (Hextend) on coagulation in patients undergoing major abdominal surgery. METHODS Patients were allocated randomly to receive Hextend) (n=21), lactated Ringer's solution (RL, n=21) or 6% HES with a low MW (130 kDa) and a low DS (0.4) (n=21). The infusion was started after induction of anaesthesia and continued until the second postoperative day to maintain central venous pressure between 8 and 12 mm Hg. Activated thrombelastography (TEG) was used to assess coagulation. Different activators were used (extrinsic and intrinsic activation of TEG) and aprotinin was added to assess hyperfibrinolytic activity (ApTEG). We measured onset of coagulation [coagulation time (CT=reaction time, r)], the kinetics of clot formation [clot formation time (CFT=coagulation time, k)] and maximum clot firmness (MCF=maximal amplitude, MA). Measurements were performed after induction of anaesthesia, at the end of surgery, 5 h after surgery and on the mornings of the first and second days after surgery. RESULTS Significantly more HES 130/0.4 [2590 (SD 260) ml] than Hextend) [1970 (310) ml] was given. Blood loss was greatest in the Hextend) group and did not differ between RL- and HES 130/0.4-treated patients. Baseline TEG data were similar and within the normal range. CT and CFT were greater in the Hextend) group immediately after surgery, 5 h after surgery and on the first day than in the two other groups. ApTEG MCF also changed significantly in the Hextend) patients, indicating more pronounced fibrinolysis. Volume replacement using RL caused moderate hypercoagulability, shown by a decrease in CT. CONCLUSION A modified, balanced high-molecular weight HES with a high degree of substitution (Hextend) adversely affected measures of coagulation in patients undergoing major abdominal surgery, whereas a preparation with a low MW and low DS affected these measures of haemostasis less. Large amounts of RL decreased the coagulation time.
Characteristics of risk in patients of nurse practitioner safety net practices

Purpose: The purpose of this article is to describe the characteristics of vulnerability in patients at safety net practices where nurse practitioners (NPs) provide the majority of the health care and to discuss the implications regarding patient data collection for all NPs who provide safety net care. Data Sources: Data were collected from patients at four safety net practices: a rural health clinic, a rural nurse‐managed health clinic, an urban nurse‐managed health clinic, and an urban Indian health clinic. Conclusions: The users of these practices reflected a significant degree of risk for poor health outcomes based on characteristics of risk identified in the literature. Patients seen in nurse‐managed health clinics without federal subsidies had more risk that those in federally supported clinics. Implications for Practice: Given the increasing evidence of the impact of social and environment factors on poor health outcomes, the importance of assessing and describing the characteristics of risk is discussed. These data are essential for supporting the value of these NP‐provided safety net services to healthcare insurers and federal health policy makers.
Electron Microscopy Analysis of the Nucleolus of Trypanosoma cruzi

The nucleolus is the main site for synthesis and processing of ribosomal RNA in eukaryotes. In mammals, plants, and yeast the nucleolus has been extensively characterized by electron microscopy, but in the majority of the unicellular eukaryotes no such studies have been performed. Here we used ultrastructural cytochemical and immunocytochemical techniques as well as three-dimensional reconstruction to analyze the nucleolus of Trypanosoma cruzi, which is an early divergent eukaryote of medical importance. In T. cruzi epimastigotes the nucleolus is a spherical intranuclear ribonucleoprotein organelle localized in a relatively central position within the nucleus. Dense fibrillar and granular components but not fibrillar centers were observed. In addition, nuclear bodies resembling Cajal bodies were observed associated to the nucleolus in the surrounding nucleoplasm. Our results provide additional morphological data to better understand the synthesis and processing of the ribosomal RNA in kinetoplastids.
Supervision to prevent childhood unintentional injury

Caregiver developmental knowledge was tested as a moderator of the association between caregiver-perceived self-efficacy and perceived supervision to prevent childhood unintentional injury. Caregivers (N = 123; 95 mothers and 28 fathers) of children (M = 3.5 ± 1.2 years, 49.6% female, 80.8% Caucasian) were recruited from pediatric primary care offices and local message boards. All caregivers completed self-report questionnaires on perceived self-efficacy, developmental knowledge, and perceived supervision to prevent injury. Separate hierarchical linear regression models were conducted to test moderation for mothers and fathers. The interaction effect of perceived self-efficacy and developmental knowledge significantly predicted maternal-perceived supervision, R 2 change = . 06, F(1, 86) = 6.76, p < . 01. No significant findings were detected for fathers. Models of studying injury prevention that consider complex cognitive-behavioral interactions and their potential modifiable role in the development of injury-prevention practices may elucidate upon the attitude–practice gap currently identified in the literature.
Many-server diffusion limits for G/Ph/n+GI queues

This paper studies many-server limits for multi-server queues that have a phasetype service time distribution and allow for customer abandonment. The rst set of limit theorems is for critically loaded G=Ph=n + GI queues, where the patience times are independent, identically distributed following a general distribution. The next limit theorem is for overloaded G=Ph=n + M queues, where the patience time distribution is restricted to be exponential. We prove that a pair of diusion-scaled total-customer-count and server-allocation processes, properly centered, converges in distribution to a continuous Markov process as the number of servers n goes to innity. In the overloaded case, the limit is a multi-dimensional diusion process, and in the critically loaded case, the limit is a simple transformation of a diusion process. When
The Effects of Social Class and Dental Attendance on Oral Health

The relationship between socio-economic status (SES) and oral health is well-established. We investigated whether the association between SES and the number of sound teeth in adults is explained by dental attendance patterns, in turn determined by the effect of SES on barriers to dental attendance. Data on 3817 participants from the 1998 Adult Dental Health Survey in the UK were analyzed. Using structural equation modeling, we found a model with 4 factors (aging, SES, attendance-profile, and barriers-to-dental-attendance) providing an adequate fit to the covariance matrix of the 9 covariates. The final model suggests that the association between SES and the number of sound teeth in adults in the UK is partially explained by the pathway [SES → barriers-to-dental-attendance → dental-attendance-profile → number-of-sound-teeth]. A direct relationship, SES → number-of-sound-teeth, is also significant.
Association study between C7673T polymorphism in apolipoprotein B gene and cerebral infarction with family history in a Chinese population.

BACKGROUND Apolipoprotein B (ApoB) levels have been shown to be associate with risk of ischemic stroke. The apolipoprotein B gene (APOB) polymorphisms may influence levels of ApoB and risk of ischemic stroke, but whether they are associated with risk of ischemic cerebral infarction (CI) with family history (CIFH) or not is unknown. AIMS To investigate the possible association of the polymorphism of APOB C7673T with CIFH in Han Chinese from Changsha. SETTINGS AND DESIGN The study population included 47 patients with CIFH and 83 patients with cerebral infarction with no family history (CINFH). Control population included 100 healthy subjects with no history of ischemic stroke matched for age, sex, and ethnic background. MATERIALS AND METHODS The APOB C7673T polymorphism was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Plasma lipids levels were also examined by standard enzymatic methods and enzyme-linked immunosorbent assay (ELISA). STATISTICS The statistical analysis was done using SPSS 14.0 software package. A P value < 0.05 was taken as significant. RESULTS This study showed an association between the APOB C7673T polymorphism and CI, especially CIFH in Han Chinese from Changsha. The T alleles frequency of the C7673T polymorphism was significantly higher in both the CIFH and CINFH groups when compared with the control group (P < 0.01; P < 0.05; respectively). And the T allele frequency in CIFH group (0.17) was higher than CINFH (0.09) group (P = 0.056). In both the CIFH and CINFH groups the serum levels of TC and LDL cholesterol were significantly high in the TT and TC genotypes than that in the CC genotype (P < 0.01), while the serum level of HDL cholesterol in the TT and TC genotypes was significantly lower than that in the CC genotype (P < 0.05). CONCLUSIONS The APOB C7673T polymorphism is related to CI, especially CIFH through changing serum lipid levels in a Chinese population.
A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord

An antiserum was raised in rabbits immunized with octopamine conjugated to thyroglobulin. The specificity of this antiserum for octopamine is shown by dot blot immunoassay analysis. The antiserum does not crossreact with dopamine, noradrenaline, and serotonin, but slight crossreactivity with the amine tyramine at high concentrations was observed. The tyramine crossreactivity could be eliminated by preabsorption with a tyramine‐glutaraldehyde‐BSA conjugate. Using this antiserum, we describe the topographical distribution of octopamine‐immunoreactive (ir) neuronal elements in wholemounts and paraffin sections of the ventral nerve cord of the American cockroach. The pattern of octopamine immunostaining is completely different from that obtained with an antidopamine serum, and can be blocked by preabsorbing the antioctopamine serum with BSA‐conjugated octopamine.
BOOTSTRAP-BASED PENALTY CHOICE FOR THE LASSO , ACHIEVING ORACLE PERFORMANCE

In theory, if penalty parameters are chosen appropriately then the lasso can eliminate unnecessary variables in prediction problems, and improve the performance of predictors based on the variables that remain. However, standard methods for tuning-parameter choice, for example techniques based on the bootstrap or cross-validation, are not sufficiently accurate to achieve this level of precision. Until Zou’s (2006) proposal for an inversely-weighted lasso, this difficulty led to speculation that it might not be possible to achieve oracle performance using the lasso. In the present paper we show that a straightforward application of the m-out-of-n bootstrap produces adaptive penalty estimates that confer oracle properties on the lasso. The application is of interest in its own right since, unlike many uses of the m-out-of-n bootstrap, it is not designed to estimate a non-normal distribution; the limiting distributions of regression parameter estimators are normal. Instead, the m-out-of-n bootstrap overcomes the tendency of the standard bootstrap to confound the errors committed in determining whether or not a parameter value is zero, with estimation errors for nonzero parameters.
Spectral Analysis of the Cerebral Activity during Voluntary Modulation of Mental States - A High Resolution EEG Study

In the neuroscience field, the use of advanced techniques of EEG recording and analysis, led to look for adequate methodology to prevent type I errors, which occur in computing thousands of univariate tests in order to highlight the brain areas in which significant activity arises. In this paper we illustrate the capability of tracking the brain activity during tasks consisting in tennis playing imagery and spatial navigation imagery, by using advanced high resolution EEG methodology accompanied by the use of appropriate statistical techniques that takes into account the risk of the Type I errors. Results showed that in the Spatial Navigation condition the power spectra activity is significantly different from the rest in the bilateral parietal areas and left motor area, while in the Tennis condition the cortical activity differs from the rest in bilateral parietal areas and in the left sensory-motor cortex. These preliminary findings are in partial accordance with previous hemodynamic studies.
Evaluating organizational efficiency resulting from information technology investment: an application of data envelopment analysis

Abstract. Even during this recessionary era, information technology (IT) expenditure in most organizations continues to grow at a high rate. Because of this increased IT expenditure, more and more senior managers are demanding that IT play a greater role in determining their firm's success by helping them increase organizational efficiencies and perhaps even achieve competitive advantage. The existing information systems literature on IT investment and organizational strategic performance, however, provides very little help to senior managers in making before‐the‐fact IT investment decisions. The present research study puts forth some ‘hard’ evidence relating IT investment to organizational strategic and economic performance by using data envelopment analysis (DEA). Eight IT investment measures were used as inputs and 10 organizational strategic and economic performance ratios were used as outputs for the DEA model. The results indicate that two‐thirds of the organizations in this research study are deemed efficient by DEA. A clear distinction exists between the efficient group and the inefficient group in terms of IT investment and organizational strategic and economic performance. The firms in the efficient group had a much higher return on their information technology investment than the inefficient group. In addition, the DEA results pinpoint the inefficient inputs and deficient outputs for an inefficient firm allowing a senior manager to take corrective actions to compensate for the situation.
Differences in Mexican American and Non-Hispanic White veterans' homocysteine levels.

PURPOSE To compare homocysteine (Hcy) levels and possible modulatory factors, such as nutrient or supplement intake, between Mexican American and Non-Hispanic White (NHW) male military veterans scoring at high- versus low-risk for stroke. DESIGN A cross-sectional survey with a high and low stroke risk biomarkers substudy. METHODS Voluntary participants were Mexican American (n=109) and NHW (n=120) veteran outpatients 54 to 85 years of age at a Southwestern Veterans Administration (VA) medical center. Measures included food frequency, health history, and stroke risk scale derived from the Framingham Study. Biomarker subgroups, 30 Mexican American and 30 NHW, half of each group scoring high or low on stroke risk, who were tested for morning fasting blood levels of Hcy, B12, and folate. FINDINGS In the cross-sectional study (n=229), nutrient intake was comparable between ethnic groups. In the substudy, Mexican Americans (n=30) with high or low stroke risk scores and NHW (n=30) with high stroke risk scores had elevated Hcy levels (12.5; 11.9; 11.4 micromol/L respectively) compared to NHW veterans with low stroke risk scores (7.8 micromol/L) even after controlling for age, education, folate, diabetes, and smoking pack-years (p=.001). Mexican Americans compared to NHW were significantly more likely to be in the preclinical (17% versus 3% >10 micromol/L) and clinical ranges (69% versus 35% >15 micromol/L) for Hcy. CONCLUSIONS Mexican Americans showed higher levels of Hcy whether they scored high or low for stroke, and greater representation in clinical and preclinical Hcy ranges compared to NHW veterans. The Framingham-derived, predominantly NHW population-based stroke risk measure might require ethnically relevant stroke risk factors for Mexican Americans.
A Generalized Image Scene Decomposition-Based System for Supervised Classification of Very High Resolution Remote Sensing Imagery

Very high resolution (VHR) remote sensing images are widely used for land cover classification. However, to the best of our knowledge, few approaches have been shown to improve classification accuracies through image scene decomposition. In this paper, a simple yet powerful observational scene scale decomposition (OSSD)-based system is proposed for the classification of VHR images. Different from the traditional methods, the OSSD-based system aims to improve the classification performance by decomposing the complexity of an image’s content. First, an image scene is divided into sub-image blocks through segmentation to decompose the image content. Subsequently, each sub-image block is classified respectively, or each block is processed firstly through an image filter or spectral–spatial feature extraction method, and then each processed segment is taken as the feature input of a classifier. Finally, classified sub-maps are fused together for accuracy evaluation. The effectiveness of our proposed approach was investigated through experiments performed on different images with different supervised classifiers, namely, support vector machine, k-nearest neighbor, naive Bayes classifier, and maximum likelihood classifier. Compared with the accuracy achieved without OSSD processing, the accuracy of each classifier improved significantly, and our proposed approach shows outstanding performance in terms of classification accuracy.
Reducing the Intrusiveness of Energy and Water End-Use Disaggregation via Social Media and Users Interactions

Water end-use disaggregation from smart-metered water consumption data is key to design water demand management strategies. Yet, despite large investments for smart metering networks, few water utilities are actually exploiting the potential of end-use-based consumer profiling. This is mostly due to the intrusiveness of existing disaggregation algorithms, which require intensive and time consuming human interaction for extracting appliance-specific information and estimating the end-use patterns. We propose to explore the potential for social media in facilitating the interactions between water utilities and water users in order to collect consumption diaries. The evaluation of the value of this user-generated information is performed by using the collected diaries as input for a disaggregation algorithm, which is shown to attain acceptable performance in reproducing the end-use patterns.
Determination of Ammonia Nitrogen in Wastewater Using Electronic Nose

A low cost, fast and convenient method based on electronic nose for determination of ammonia nitrogen in wastewater is developed in this study. The pH of the sample is adjusted to higher than 11 by adding saturated sodium hydroxide solution, through which most of the ammonium ions transform into ammonia and volatilize to the gas phase following Henry's law. A sensor array consist of four metal oxide semiconductor gas sensors responses to the headspace gas of the sample. Response data is analyzed using BP neural network. The result shows a robust relationship between the responses patterns of the electronic nose and the wastewater ammonia nitrogen concentrations.