Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcription Proﬁling-Based Identiﬁcation of Staphylococcus aureus Genes Regulated by the agr and/or sarA Loci

The advent of transcription proﬁling technologies has provided researchers with an unprecedented ability to study biological processes. Accordingly, a custom-made Affymetrix GeneChip, constituting >86% of the Staphylococcus aureus genome, was used to identify open reading frames that are regulated by agr and/or SarA, the two best-studied regulators of the organism’s virulence response. RNA extracted from wild-type cells and agr , sarA , and agr sarA mutant cells in the early-, mid-, and late-log and stationary phases of growth was analyzed. Open reading frames with transcription patterns expected of genes either up- or downregulated in an agr -and/or SarA-dependent manner were identiﬁed. Oligonucleotide microarray and Northern blot analyses conﬁrmed that the transcription of several known virulence genes, including hla (alpha-toxin) and spa (protein A), is regulated by each effector and
MYELOID NEOPLASIA Subclonal mutations in SETBP1 confer a poor prognosis in juvenile myelomonocytic leukemia

disorders. These lesions were detected in nearly 10% of patients with JMML and have been characterized as secondary events. We hypothesized that rare subclones with SETBP1 mutations are present at diagnosis in a large portion of patients who relapse, but are below the limits of detection for conventional deep sequencing platforms. Using droplet digital polymerase chain reaction, we identified SETBP1 mutations in 17/56(30%) of patientswho were treated in the Children’sOncology Group sponsored clinical trial, AAML0122. Five-year event-free survival in patients with SETBP1 mutations was 18% 6 9% compared with 51% 6 8% for those without mutations ( P 5 .006). ( Blood . 2015;125(3):516-524)
Edinburgh Research Explorer Post-transcriptional control of nuclear-encoded cytochrome oxidase subunits in Trypanosoma

Trypanosomes represent an excellent model for the post-transcriptional regulation of gene expression because their genome is organized into polycistronic transcription units. However, few signals governing developmental stage-specific expression have been identified, with there being no compelling evidence for widespread conservation of regulatory motifs. As a tool to search for common regulatory sequences we have used the nuclear-encoded components of the cytochrome oxidase (COX) complex of the trypanosome respiratory chain. Components of this complex represent a form of post-transcriptional operon because trypanosome mitochondrial activity is unusual in being developmentally programmed. By genome analysis we identified the genes for seven components of the COX complex. Each mRNA exhibits bloodstream stage-specific instability, which is not mediated by the RNA silencing pathway but which is alleviated by cycloheximide. Reporter assays have identified regulatory regions within the 3 0 -untranslated regions of three COX mRNAs operating principally at the translational level, but also via mRNA stability. Interrogation of the mapped regions via oligonucleotide frequency scoring provides evidence for genome-wide conservation of regulatory sequences among a large cohort of procyclic-enriched transcripts. Analysis of the co-regulated subunits of a stage-specific enzyme is therefore a novel approach to uncover cryptic regulatory sequences controlling gene expression at the post-transcriptional level.
Security Enhancement using Dynamic Cache on DSR Based MANETS

: In the DSR Routing Algorithm, when a node needs to transmit a packet to some other node it has to find the route every time. If the route is not found, then the node generates a route request (RREQ) which has (Source id, destination id, sequence number and route number). When a node receives a RREQ , the node searches for available routes to the destination from its route cache. If it finds an available route, a node sends a route reply packet (RREP), which contains route information from the source to the destination, to the source. Otherwise, it appends its id to the source route in the RREQ and rebroadcasts the RREQ. Thus black hole node attacks the network at the time of route finding and start acting as if it has the correct path for the destination. This cause serious problem in the operations and services of the networks . They may lead to the problem of system failure in terms of network availability. It makes the ad hoc node unable to transmit and receive information. This eventually brings down the overall performance of the network. Current solutions may either found the intrusion too late or produce a heavy network overhead . Many solutions are assuming as if there is only one black hole node in the network but black hole attack can be cooperative. A black hole attack and DOS attack increases network overhead, decreases the network’s lifetime by boosting energy consumption, and finally destroys the network. We are representing the solution for above problem
Adenoviral Gene Transfer Allows Smad-Responsive Gene Promoter Analyses and Delineation of Type I Receptor Usage of Transforming Growth Factor- (cid:1) Family Ligands in Cultured Human Granulosa Luteal Cells

In the human ovary, cell growth and differentiation are reg- ulated by members of the TGF- (cid:1) superfamily, including growth differentiation factor-9 (GDF9), TGF- (cid:1) and activin. TGF- (cid:1) and activin are known to signal via Smad3 activation, and we have recently shown the involvement of Smad3 in cellular responses to GDF9. Recent studies with Smad3-defi-cient mice have also indicated a key role for this signaling mediator in ovarian folliculogenesis. We now demonstrate the use of a Smad3 reporter (CAGA-luciferase) adenovirus in pri- mary cultures of human granulosa-luteal (hGL) cells to detect GDF9, TGF- (cid:1) , and activin responses. In rodent granulosa cells, TGF- (cid:1) and GDF9 signal through the TGF- (cid:1) type I receptor or activin receptor-like kinase 5 (Alk5), whereas the effect of activin is mediated though the activin type IB receptor, also known as Alk4. We now show that the GDF9 response in hGL cells is markedly potentiated upon overexpression of Alk5 by adenoviral gene transduction, as measured by the CAGA-luciferase reporter activity. A similar response to Alk5 overexpression was observed for TGF- (cid:1) , but not for activin. Adenoviral overexpression of the activin type IB receptor Alk4 in hGL cells specifically potentiated activin signaling, but not GDF9 or TGF- (cid:1) signaling. Alk5 overexpression in hGL cells also potentiated the GDF9 response when inhibin B production was used as the read-out. These results indicate that the CAGA-luciferase adenovirus can be used to study Smad3 signaling in primary cultures of human cells, and that adeno- viral overexpression of wild-type receptors of the TGF- (cid:1) superfamily can be used to amplify the cellular response to li- gands such as GDF9, TGF- (cid:1) , and activin. Furthermore, these studies indicate the involvement of Alk5 in GDF9 signaling in human cells and therefore, along with other recent studies, highlight how a limited number of type I and II receptors cooperate to generate specificity of action within the TGF- (cid:1) superfamily. ( J Clin Endocrinol Metab 90: 271–278, 2005) ,
This information is current as and Membrane-Associated Antigens Signalosomes in Response to Both Soluble Formation of Polarized B Cell Receptor Actin Reorganization Is Required for the

B cells encounter both soluble Ag (sAg) and membrane-associated Ag (mAg) in the secondary lymphoid tissue, yet how the physical form of Ag modulates B cell activation remains unclear. This study compares actin reorganization and its role in BCR signalosome formation in mAg- and sAg-stimulated B cells. Both mAg and sAg induce F-actin accumulation and actin polymerization at BCR microclusters and at the outer rim of BCR central clusters, but the kinetics and magnitude of F-actin accumulation in mAg-stimulated B cells are greater than those in sAg-stimulated B cells. Accordingly, the actin regulatory factors, coﬁlin and gelsolin, are recruited to BCR clusters in both mAg- and sAg-stimulated B cells but with different kinetics and patterns of cellular redistribution. Inhibition of actin reorganization by stabilizing F-actin inhibits BCR clustering and tyrosine phosphorylation induced by both forms of Ag. Depolymerization of F-actin leads to unpolarized microclustering of BCRs and tyrosine phosphorylation in BCR microclusters without mAg and sAg, but with much slower kinetics than those induced by Ag. Therefore, actin reorganization, mediated via both polymerization and depolymerization, is required for the formation of BCR signalosomes in response to both mAg and sAg. The Journal of Immunology , 2012, 188: 000–000.
Effect of Brewer’s yeast and soya bean cake on brood rearing, pollen gathering and honey yield in honey bee colonies.

This work was conducted in the Apiary of Plant Protection Institute at Quantar, Qaluobia to study the effect of additive pollen substitute feeding of honey bee colonies on brood rearing, queen’s egg laying, pollen gathering and honey production through the period of 1/3/2012 to 29/08/2012. Results revealed that feeding the honey bee colonies on additive pollen substitute and sugar syrup (1:1) make honey bee colonies have significantly more brood rearing, queen’s egg laying, pollen gathering and honey production than the control colonies which fed with sugar syrup only.
CONTRAST THE HISTOPATHOLOGICAL IMPACTS OF ACEMANNAN WITH THAT OF FORMOCRESOL AS MASH DRESSING SPECIALISTS IN ESSENTIAL TEETH WENT THROUGH PULPOTOMY

: Aim: The current exploration was directed to relate histopathological effects of acemannan with those of primary cresol, as experts in biting dressings for basic teeth went through pulpotomy. Methods: Weight partitions (WPM) containing neighborhood components of polysaccharide and glycoprotein were picked up utilizing the hyper-dry ensured structure in the wake of cleaning the booted materials through running water from the Aloe Vera gel slurry. 48 first fundamental teeth of 6-8-year-old, who had made plans for successive extraction sometime prior, were chosen. Our current research was conducted at Services Hospital, Lahore from April 2019 to March 2020. The posterity stayed separated into two equal gatherings: twelve teeth each. Pulpotomy was acted in commonly sets, where Group I was treated by AHM, acemannan, as the dressing subject matter expert, while primary cresol was utilized as the dressing administrator in Group II. Teeth from each gathering were taken out at two, four and half a month stretches for histopathological appraisal. Results: In Set I (Acemannan), subsequently thirteen weeks, more advantageous results regarding deteriorating of pounding, insufficient advancement of the dentinal association and relationship of sensitive tissues were appeared (p < 0.07). Most of the acemannan-treated gatherings indicated no pound disturbance, with slight deteriorating of pulverize underneath the site of presentation (4/17, 27%) and just 1 of every 13 teeth (10.6%) demonstrated partial attitude of the dentinal association. Alternately, Group II (molded cresol) demonstrated moderate to outrageous deteriorating of squash (11/13, 87%), with no dentine association demeanor noticed. In basically all cases (12/16, 87%), the sensitive squash tissue was not viable. Conclusion: Based on these ends, AHM, acemannan could remain the sensible expert for pulpotomy of fundamental teeth. Likewise, the fire response was less cut and no rot was found after pulpotomy with acemannan related to underlying cresol.
INCIDENCE OF SALMONELLAE IN CHILLED CHICKEN CARCASSES IN RETAILS PORT-SAID CITY

_________________ ________________________________________ __________________ A total of 120 samples were collected from retail markets from November 2011 to February 2012 . Salmonella spp. was detected in 14 (11.7 %) of the samples analyzed. Among the chicken samples examined giblets had higher contamination level of Salmonella 7 (5.8 %) while the meat samples had lower percent of contamination 3(2.5%). Out of 14 Salmonella isolates, 5 different serotypes were identified S. typhimurium (42.9) was the most frequent followed by S. enteritidis 21.4%, S. virchow 21.4%, S.anatum 7.1% and Salmonella type (cid:23) 7.1%. The results of the present study indicated that there was a high level of Salmonella contamination of chicken meat and giblets in retail markets, which could be considered as one of the major potential source of human salmonellosis in Port-Said, city. ___________________________________________________________________________
A Genetic Programming Approach to Identifying the Workers Ability Impacts on Safety Execution in Fireworks Industry

: This study uses Genetic Programming (GP)-based modelling to determine and propose the type of task that a specific worker with certain fundamental characteristics is considered suitable for in a fireworks industrial unit in order to improve industrial safety in the process. It seeks to identify and suggest for safety execution for the kind of process in the fireworks industry. The basic characteristics of firework industry workers are taken into consideration of age, experience, task training, Personal protective equipment (PPE) knowledge, awareness training and history of accidents. The data set were collected from the survey based questionnaire. The data is divided into three categories of set I, set II and set III. , each with mix of above listed characteristics. A GP model is built for each set of data. A genetic algorithm which selects the best combination of worker’s ability for ensure the safety to attain the low risk level of each candidate safety measure. Predict the best fitness of training, testing and validation on set III is 0.3315,0.5062,0.4972 respectively. The model produces outcomes to recommend for understand the task, avoid the unsafe conditions, regardless of worker’s characteristics.
Constraint-Induced Movement Therapy for the Paretic Lower Limb in Acute and Sub-Acute Stroke

Stroke is currently one of the major causes of physical incapacity worldwide. Patients with stroke often exhibit impaired movements, frequently aggravated by conditioned suppression of the use of the affected segments (learned disuse). Constraint-Induced Movement Therapy (CIMT), developed from animal experiments in the 1950s and 60s, has proven to be quite efficient in rehabilitating the paretic upper limb after unilateral pathologies, such as stroke. This therapy seeks to stimulate the use of the affected segment, reducing the effects of learned disuse and helping to promote positive changes in brain structure and function. CIMT was also adapted to treat the paretic lower limb, but there are still fewer studies when compared to the upper limb. After stroke, applying this technique to lower limbs has demonstrated good results, with important effects on motor function and functional activities performed with these limbs. In the early phases after stroke, CIMT contributes to acquiring adequate movement patterns in the lower limbs, acting in the phase with the greatest cerebral plasticity to promote better recovery. However, CIMT to lower limbs is more commonly applied in the chronic phase of the disease, similar to the upper limbs. This review discusses the use of CIMT in the lower limbs after stroke, with emphasis on the acute and subacute phases of the disease. as in therapy for the upper allows more equal weight transfer lower
A novel protective function for cytokinin in the light stress response is mediated by the AHK2 and AHK3 receptors

One sentence summary: Cytokinin protects plants from the consequences of light stress by acting on ROS scavenging and D1 protein levels, thereby preventing photoinhibition. Abstract Cytokinins are plant hormones that regulate diverse processes in plant development and responses to biotic and abiotic stresses. In this study we show that Arabidopsis thaliana plants with a reduced cytokinin status, i.e. cytokinin receptor mutants and transgenic cytokinin-deficient plants, are more susceptible to light stress compared to wild-type plants. This was reflected by a stronger photoinhibition after 24 hours high light (~1000 µmol m -2 s -1 ), as shown by the decline in maximum quantum efficiency of photosystem II photochemistry (Fv/Fm). Photosystem II, especially the D1 protein, is highly sensitive to the detrimental impact of light. Therefore, photoinhibition is always observed when the rate of photodamage exceeds the rate of D1 repair. We demonstrate that in plants with a reduced cytokinin status the D1 protein level was strongly decreased upon light stress. Inhibition of the D1 repair cycle by lincomycin treatment indicated that these plants experience a stronger photodamage. The efficiency of photoprotective mechanisms, such as non-enzymatic and enzymatic scavenging systems, was decreased in plants with a reduced cytokinin status which could be a cause for the increased photodamage and subsequent D1 degradation. Additionally, slow and incomplete recovery in these plants after light stress indicated insufficient D1 repair. Mutant analysis revealed that the protective function of cytokinin during light stress depends on the AHK2 and AHK3 receptors and the type-B response regulators ARR1 and ARR12. We conclude that proper cytokinin signaling and regulation of specific target genes is necessary to protect leaves efficiently from light stress.
How to treat the troll? An empirical analysis of counterproductive online behavior, personality traits and organizational behavior

Purpose – Online environments, such as social networks and online forums, offer new possibilities and a wide variety of identity and social relationship management for the users. However, besides functional contributions like mutual support and easy ways of establishing contacts there are critical perspectives on computer-mediated communication (CMC) regarding detrimental behavior like provoking, overbearing, attacking and insulting other users, especially when anonymity is high. Recent research has shown that these kinds of online behavior are associated with personality traits like sadism, machiavellianism, narcissism, and psychopathy (Buckels, Trapnell & Paulhus, 2014) and can lead to severe trouble, negative affect and dysfunction in online communities (Cheng, Danescu-Niculescu-Mitzil & Leskovec, 2015). As such, in the public perception “trolls“ have become a synonym for counterproductive and dysfunctional behavior (Bishop, 2014a, 2014b). Our research aim was to shed more light on trolling and counterproductive online behavior theoretically as well as empirically. In other words: We wanted to know who is behind the troll? How can he or she be characterized in terms of personality traits and what can be expected from trolls when it comes to the organizational context and job performance? Design/methodology/approach – In a first step, we formulated a theoretical framework on counterproductive online behavior. On that ground, two online surveys (N = 122; N = 133) were conducted. The first study’s goal was to develop and validate a questionnaire on counterproductive online behavior. The second study analyzed counterproductive online behavior and tested for possible interrelations to personality traits and work-related outcomes. Originality/value – Using explanatory factor analyses we developed a 40-item questionnaire with two higher dimensions: Constructiveness and destructiveness. 15 subscales focus on different communication styles and trolling strategies. The second study tested the two dimensions of
Study the Effect of Weight Loss in Improvement of Hepatic Fibrosis in Patients with Chronic HCV

Background: Liver fibrosis considered as a reversible disease, but curing the primary hepatic disease, sometimes, doesn’t mean fibrosis improvement. Steatosis could be one of the reasons responsible for this failure. Weight loss has been shown to improve fibrosis, but few data is available from Egypt. Objective: to test the effect of weight reduction on the degree of fibrosis improvement in patients with chronic hepatitis C (CHC). Methods: Adult patients (> 18 years) were recruited from hepatitis Cout patient’s clinic, National Liver Institute (NLI), University of Menoufyia, from 2016 to 2018. Results: Thirty CHC patients with moderate and severe steatosis, who were initially assessed by liver biopsy and Transient elastography (TE) for their fibrosis stage, were followed up by Transient elastography after losing >10% of their weight. Median age was 44.7 years (29 – 59 years) and 50% were men. The degree of fibrosis and its values improved with the significant weight loss (p < 0.01 for both). Conclusion: Weight reduction is associated with improvement of hepatic fibrosis in patient with CHC. Elastography; NAFLD: Nonalcoholic Fatty Liver Disease; N: Number, SD: Standard Deviation, BMI: Body Mass Index; ALT: Alanine Aminotransferase, AST: Aspartate Aminotransferase, RNA: Ribonucleic Acid, INR: International Normalized Ratio, kPa: Kilopascal
Stochastic traffic modelling and decentralised signal control based on a state transition probability model

In this study, the authors develop a state transition probability model for an isolated intersection on the basis of link-based state transition probability matrix. The state of an intersection is defined as the combination of its input links’ states which represent their respective congestion level by occupancy rate. This way of modelling is further extended to a compact region by likening the region to a virtual node with several virtual input links to avoid dimension expansion. Based on the state transition probability model, the stochastic signal control problem for both intersections and compact regions is formulated as a Markov decision process with the specified definition of state, action, probability and reward. A sensitivity-based policy iteration algorithm is employed to solve the Markov decision process in real-time, which has a great advantage in computational efficiency. The results of the numerical study on a calibrated network of Caohejing District in Shanghai indicate that the authors’ proposed method outperforms the fixed-time and actuated signal control at high loads in terms of many indices and greatly decreases the variability in the traffic performance. In addition, the compact region control can improve the optimisation efficiency while providing similar performance to the intersection control at different loads.
Case Report Implant-Supported Overdenture with a Custom Cad/ Cam-Fabricated Bar Attachment in Combination with Antirotational Locator® Attachments in a Severely Atrophied Mandible: A Case Report

Background : An atrophied mandible, inserting two implants in the edentulous mandible is an established way to improve prosthesis stability. Multiple options for subsequent retention of the denture are available. Methods: For oral rehabilitation, a modified bar supplemented by antirotational Locator® was used for prosthesis anchoring. This solution ensured and improved retention, despite of the reduced bone supply associated with severe infraposition of the floor of the mouth and limited vertical space. Results: The surgical and prosthetic procedures provided high denture stability and good chewing ability Conclusion: It can be concluded that the technique described allows good clinical outcome with minimal complications. It can be recommended to restore edentulous and atrophied mandible.