Miracle-dz/UniFAR-FTUs huggingface dataset:
N-acetylcysteine reverses the decrease of DNA methylation status caused by engineered gold, silicon, and chitosan nanoparticles

Introduction: Engineered nanoparticles (ENPs) are one of the most widely used types of nanomaterials. Recently, ENPs have been shown to cause cellular damage by inducing ROS (reactive oxygen species) both directly and indirectly, leading to the changes in DNA methylation levels, which is an important epigenetic mechanism. In this study, we investigated the effect of ENP-induced ROS on DNA methylation. Materials and methods: Human embryonic kidney and human keratinocyte (HaCaT) cells were exposed to three different types of ENPs: gold nanoparticles, silicon nanoparticles (SiNPs), and chitosan nanoparticles (CSNPs). We then evaluated the cytotoxicity of the ENPs by measuring cell viability, morphology, cell apoptosis, cell proliferation, cell cycle distribution and ROS levels. Global DNA methylation levels was measured using 5-methylcytosine immunocytochemical staining and HPLC analysis. DNA methylation levels of the transposable elements, long interspersed element-1 (LINE-1) and Alu, were also measured using combined bisulfite restriction analysis technique. DNA methylation levels of the TEs LINE-1 and Alu were also measured using combined bisulfite restriction analysis technique. Results: We found that HaCaT cells that were exposed to SiNPs exhibited increased ROS levels, whereas HaCaT cells that were exposed to SiNPs and CSNPs experienced global and Alu hypomethylation, with no change in LINE-1 being observed in either cell line. The demethylation of Alu in HaCaT cells following exposure to SiNPs and CSNPs was prevented when the cells were pretreated with an antioxidant. Conclusion: The global DNA methylation that is observed in cells exposed to ENPs is associated with methylation of the Alu elements. However, the change in DNA methylation levels following ENP exposure is specific to particular ENP and cell types and independent of ROS, being induced indirectly through disruption of the oxidative defense process.
Learning Hierarchical Representations for Face Recognition using Deep Belief Network Embedded with Softmax Regress and Multiple Neural Networks

In face recognition and classification, feature extraction and classification based on insufficient labeled data is a well-known challenging problem. In this paper, a novel semi-supervised learning algorithm named deep belief network embedded with Softmax regress (DBNESR) is proposed to address this problem. DBNESR first learns hierarchical representations of feature by deep learning and then makes more efficient classification with Softmax regress. At the same time we design many kinds of classifiers based on supervised learning: BP, HBPNNs, RBF, HRBFNNs, SVM and multiple classification decision fusion classifier——hybrid HBPNNs-HRBFNNs-SVM classifier. The conducted experiments validate: Firstly, the proposed semi-supervised deep learning algorithm DBNESR is optimal for face recognition with the highest and most stable recognition rates; Second, the semi-supervised learning algorithm has better effect than all supervised learning algorithms; Third, hybrid neural networks has better effect than single neural network.
Stents Versus Bypass Surgery for Left Main Coronary Artery Disease: 3-Year Clinical Outcomes Depending on SYNTAX Score

The purpose of our study was to compare the 3-year of follow-up clinical outcomes in patients suffering from left main coronary artery disease (LMCAD) treated either by percutaneous coronary intervention (PCI) or by coronary artery bypass grafting (CABG) depending on SYNTAX score tertiles. The primary end point of the study was all-cause mortality for the PCI arm versus CABG arm depending on SYNTAX score tertiles. The secondary end points were the recurrence of angina pectoris following revascularization, the acute nonfatal myocardial infarction, the reduction of the left ventricular ejection fraction or the need for myocardial revascularization. With regard to patients with LMCAD, at low risk (SYNTAX score 0-22) there was no difference in the frequency of end-point occurrence among patients treated by PCI compared to CABG. Also, the tendency to increase end point occurrence in patients with LMCAD treated by PCI compared to patients treated by CABG was more evident in patients at intermediate risk, but the significant statistical difference was recorded only in the occurrence of acute myocardial infarction. Regarding the patients with LMCAD at high risk (SYNTAX score over 33) the endpoint occurrence was significantly increased, statistically significant differences were recorded in all evaluated endpoints. In conclusion, coronary artery bypass grafting remains the standard treatment for high-risk patients with complex lesions, while for patients with LMCAD at low or intermediate risk, percutaneous coronary intervention by stent implantation remains an alternative that does not pose significant risks.
Performing diagnostic radial access coronary angiography on uninterrupted direct oral anticoagulant therapy: a prospective analysis

Transradial access has long been championed as the safer route for those undergoing diagnostic coronary angiography or percutaneous coronary interventions (PCI). Numerous studies have established its overall safety even in the context of an acute ST-elevation myocardial infarction (STEMI). 1 2 However, little data exist on the safety of transradial access in those on direct oral anticoagulants (DOAC). Conventionally, many interrupt these agents prior to coronary intervention irrespective of the access site selected. There is concern, however, that interrupting anticoagulation for those at high risk poses an unacceptable risk of stroke and embolisation. There is equal concern that continued anticoagulation increases bleeding, both access and non-access sites. Several trials have already determined the safety of cardiac catheterisation with continued vitamin K antagonists.3–6 Currently, there is paucity of randomised data shaping guidelines and practice regarding continued direct oral anticoagulation prior to cardiac catheterisation. The Perioperative Anticoagulant Use for Surgery Evaluation study is an ongoing randomised prospective trial with highly anticipated results. It is designed to address the unanswered question of the safety of interrupting DOAC for atrial fibrillation before elective surgery/procedure. This study includes three parallel cohorts, one for each DOAC. The type of DOAC, renal function and surgery/procedure-related bleeding risk are factored int. The secondary aim is to determine the effect of the preprocedure interruption on residual anticoagulation when measured by the dilute thrombin time …
Quality of life in euthymic patients with unipolar major depressive disorder and bipolar disorder

Objective: This study aimed to compare quality of life (QOL) between patients with major depressive disorder (MDD) in remission and patients with bipolar disorder (BD) in remission, and to explore the relationship between QOL and demographic, clinical, and cognitive variables. Methods: This study included 49 euthymic patients with MDD, 59 euthymic patients with BD, and 52 healthy controls (HC). The 17-item Hamilton Depression Rating Scale (HAMD-17), Hamilton Anxiety Rating Scale (HAMA), and Young Mania Rating Scale (YMRS) were used to assess symptoms of depression, anxiety, and mania respectively. QOL was assessed with the Chinese version of the World Health Organization Quality of Life Scale Brief (WHOQOL-BREF). Cognitive function was assessed with the repeated neuropsychological assessment scale (RBANS). Results: Compared with HC, patients with MDD had lower overall and subdomain scores (except ENVIR) on the WHOQOL-BREF (p<0.05). The BD group had decreased overall WHOQOL-BREF scores and decreased PHYS and SOCIL subdomain scores (p<0.05). PSYCH scores were lower in patients with MDD, compared with patients with BD (p<0.05). Among patients with MDD, HAMD score was negatively correlated with all domains on the WHOQOL-BREF. Marital status was associated with an increase in subdomain scores on the PSYCH and ENVIR subdomains. In the BD group, attention on the RBANS correlated negatively with PSYCH score; age correlated negatively with SOCIL. Conclusions: QOL of patients with MDD and BD in remission is inferior to that of the normal population. QOL among MDD is inferior to that among BD. Marital status was associated with increased QOL among MDD, but not among BD. Residual symptoms related to depression or anxiety decreased QOL in both MDD and BD. More attention should be paid to the QOL of patients with mood disorders, especially MDD, even during euthymic periods.
Meta-Analysis of the Effects of the Catechol-O-Methyltransferase Val158/108Met Polymorphism on Parkinson’s Disease Susceptibility and Cognitive Dysfunction

Background: There is a continued debate and inconsistent findings in previous literature about the relationship of catechol-O-methyltransferase (COMT) and Parkinson’s disease (PD) susceptibility as well as cognitive dysfunction. To substantiate this existing gap, we comprehensively examine COMT genotype effects on the development of PD and test the hypothesis that the Met158 allele of the COMT gene is associated with cognitive dysfunction by conducting a meta-analysis review. Methods: PubMed/MEDLINE, Embase, Cochrane databases search (18/30/08) yielded 49 included studies. Data were extracted by two reviewers and included COMT genotype, publication year, diagnostic status, ancestry, the proportion of male participants, and whether genotype frequencies were consistent with Hardy–Weinberg equilibrium. Unadjusted odds ratios (ORs) were used to derive pooled estimates of PD risk overall and in subgroups defined by ethnicity, gender, and onset of disease. Moreover, the association of certain cognitive domains in PD and COMT gene type was explored. Meta-analyses were performed using random-effect models and p value–based methods. All statistical tests were two-sided. The present study was registered with PROSPERO (CRD42018087323). Results: In the current studies, we found no association between COMT Val158/108Met polymorphism and PD susceptibility. However, the gender-stratified analyses revealed marginally significant effects in heterozygote model analyses in women (P = 0.053). In addition, stratification according to onset of PD also shows significant effects of COMT Val158/108Met polymorphism on late-onset population both in recessive (P = 0.017) and allelic (P = 0.017) genetic models. For the intelligence quotient (IQ) score and Unified Parkinson Disease Rating Scale III (UPDRS III), there was no evidence for genetic association, except in subgroup analyses in Asian populations (IQ score, P = 0.016; UPDRS III, P < 0.001). Conclusion: The COMT Val158/108Met polymorphism is associated with the risk for PD in female or late-onset PD. Methionine/methionine carriers of Asian population performed significantly worse than the valine allele carriers in IQ score and UPDRS III.
Effects of Melatonin on the Defense to Acute Hypoxia in Newborn Lambs

Neonatal lambs, as other neonates, have physiologically a very low plasma melatonin concentration throughout 24 h. Previously, we found that melatonin given to neonates daily for 5 days decreased heart weight and changed plasma cortisol and gene expression in the adrenal and heart. Whether these changes could compromise the responses to life challenges is unknown. Therefore, firstly, we studied acute effects of melatonin on the defense mechanisms to acute hypoxia in the neonate. Eleven lambs, 2 weeks old, were instrumented and subjected to an episode of acute isocapnic hypoxia, consisting of four 30 min periods: normoxia (room air), normoxia after an i.v. bolus of melatonin (0.27 mg kg−1, n = 6) or vehicle (ethanol 1:10 NaCl 0.9%, n = 5), hypoxia (PaO2: 30 ± 2 mmHg), and recovery (room air). Mean pulmonary and systemic blood pressures, heart rate, and cardiac output were measured, and systemic and pulmonary vascular resistance and stroke volume were calculated. Blood samples were taken every 30 min to measure plasma norepinephrine, cortisol, glucose, triglycerides, and redox markers (8-isoprostane and FRAP). Melatonin blunted the increase of pulmonary vascular resistance triggered by hypoxia, markedly exacerbated the heart rate response, decreased heart stroke volume, and lessened the magnitude of the increase of plasmatic norepinephrine and cortisol levels induced by hypoxia. No changes were observed in pulmonary blood pressure, systemic blood pressures and resistance, cardiac output, glucose, triglyceride plasma concentrations, or redox markers. Melatonin had no effect on cardiovascular, endocrine, or metabolic variables, under normoxia. Secondly, we examined whether acute melatonin administration under normoxia could have an effect in gene expression on the adrenal, lung, and heart. Lambs received a bolus of vehicle or melatonin and were euthanized 30 min later to collect tissues. We found that melatonin affected expression of the immediate early genes egr1 in adrenal, ctgf in lung, and nr3c1, the glucocorticoid receptor, in adrenal and heart. We speculate that these early gene responses may contribute to the observed alterations of the newborn defense mechanisms to hypoxia. This could be particularly important since the use of melatonin is proposed for several diseases in the neonatal period in humans.
High Circulating Methylated DNA Is a Negative Predictive and Prognostic Marker in Metastatic Colorectal Cancer Patients Treated With Regorafenib

Background: Regorafenib improves progression free survival (PFS) in a subset of metastatic colorectal cancer (mCRC) patients, although no biomarkers of efficacy are available. Circulating methylated DNA (cmDNA) assessed by a five-gene panel was previously associated with outcome in chemotherapy treated mCRC patients. We hypothesized that cmDNA could be used to identify cases most likely to benefit from regorafenib (i.e., patients with PFS longer than 4 months). Methods: Plasma samples from mCRC patients were collected prior to (baseline samples N = 60) and/or during regorafenib treatment (N = 62) for the assessment of cmDNA and total amount of cell free DNA (cfDNA). Results: In almost all patients, treatment with regorafenib increased the total cfDNA, but decreased cmDNA warranting the normalization of cmDNA to the total amount of circulating DNA (i.e., cmDNA/ml). We report that cmDNA/ml dynamics reflects clinical response with an increase in cmDNA/ml associated with higher risk of progression (HR for progression = 1.78 [95%CI: 1.01–3.13], p = 0.028). Taken individually, high baseline cmDNA/ml (above median) was associated with worst prognosis (HR for death = 3.471 [95%CI: 1.83–6.57], p < 0.0001) and also predicted shorter PFS (<16 weeks with PPV 86%). In addition, high cmDNA/ml values during regorafenib treatment predicted with higher accuracy shorter PFS (<16 weeks with a PPV of 96%), therefore associated with increased risk of progression (HR for progression = 2.985; [95%CI: 1.63–5.46; p < 0.0001). Conclusions: Our data highlight the predictive and prognostic value of cmDNA/ml in mCRC patients treated with regorafenib.
Collagen-Based Tissue Engineering Strategies for Vascular Medicine

Cardiovascular diseases (CVDs) account for the 31% of total death per year, making them the first cause of death in the world. Atherosclerosis is at the root of the most life-threatening CVDs. Vascular bypass/replacement surgery is the primary therapy for patients with atherosclerosis. The use of polymeric grafts for this application is still burdened by high-rate failure, mostly caused by thrombosis and neointima hyperplasia at the implantation site. As a solution for these problems, the fast re-establishment of a functional endothelial cell (EC) layer has been proposed, representing a strategy of crucial importance to reduce these adverse outcomes. Implant modifications using molecules and growth factors with the aim of speeding up the re-endothelialization process has been proposed over the last years. Collagen, by virtue of several favorable properties, has been widely studied for its application in vascular graft enrichment, mainly as a coating for vascular graft luminal surface and as a drug delivery system for the release of pro-endothelialization factors. Collagen coatings provide receptor–ligand binding sites for ECs on the graft surface and, at the same time, act as biological sealants, effectively reducing graft porosity. The development of collagen-based drug delivery systems, in which small-molecule and protein-based drugs are immobilized within a collagen scaffold in order to control their release for biomedical applications, has been widely explored. These systems help in protecting the biological activity of the loaded molecules while slowing their diffusion from collagen scaffolds, providing optimal effects on the targeted vascular cells. Moreover, collagen-based vascular tissue engineering substitutes, despite not showing yet optimal mechanical properties for their use in the therapy, have shown a high potential as physiologically relevant models for the study of cardiovascular therapeutic drugs and diseases. In this review, the current state of the art about the use of collagen-based strategies, mainly as a coating material for the functionalization of vascular graft luminal surface, as a drug delivery system for the release of pro-endothelialization factors, and as physiologically relevant in vitro vascular models, and the future trend in this field of research will be presented and discussed.
Analysis, Modeling and Dynamic Optimization of 3D Time-of-Flight Imaging Systems

The present thesis is concerned with the optimization of 3D Time-of-Flight (ToF) imaging systems. These novel cameras determine range images by actively illuminating a scene and measuring the time until the backscattered light is detected. Depth maps are constructed from multiple raw images. Usually two of such raw images are acquired simultaneously using special correlating sensors. This thesis covers four main contributions: A physical sensor model is presented which enables the analysis and optimization of the process of raw image acquisition. This model supports the proposal of a new ToF sensor design which employs a logarithmic photo response. Due to asymmetries of the two read-out paths current systems need to acquire the raw images in multiple instances. This allows the correction of systematic errors. The present thesis proposes a method for dynamic calibration and compensation of these asymmetries. It facilitates the computation of two depth maps from a single set of raw images and thus increases the frame rate by a factor of two. Since not all required raw images are captured simultaneously motion artifacts can occur. The present thesis proposes a robust method for detection and correction of such artifacts. All proposed algorithms have a computational complexity which allowsreal-time execution even on systems with limited resources (e.g. embeddedsystems). The algorithms are demonstrated by use of a commercial ToF camera.
The Relationship Between Executive Functions and Academic Performance in Primary Education: Review and Meta-Analysis

The purpose of this study was to research the relationship between executive functions and academic performance in primary education (6–12 years). Based on 21 samples (n = 7,947), a meta-analysis of random effects demonstrated a moderately significant weighted effect size (r = 0.365) and was found to be a good predictor of academic performance. For the subjects of language and mathematics, the results of the random effects model were similar and slightly higher for mathematics (r = 0.350; r = 0.365). Thus, the theory that executive functions have greater influence on mathematical performance is supported, especially in aspects such as coding, organization, and the immediate retrieval of information. Regarding the different executive function components (working memory, inhibition, cognitive flexibility, and planning), working memory had the highest presence (k = 14, n = 3,740) and predictive weight for performance, with an effect size of r = 0.370 for random effects, with a moderate level of significance. The moderating effect of variables such as gender and age were also analyzed. After performing a meta-regression, gender resulted in a value of R2 = 0.49; the age variable was not significant. This result is especially important since age has traditionally been considered to be the moderating variable of executive functions. The review reveals a good predictive power of executive functions in the primary education stage, and it is even higher at the early ages, indicating its great significance in describing future performance. The study also revealed the competencies and specific aspects of the executive functions that affect the way in which its components intervene in the academic area, demonstrating the mediating effect of variables such as physical fitness, motor skills, and memory processes.
C++ AMP: Accelerated Massive Parallelism with Microsoft Visual C++

base class, in NBody case study, 30 accelerator about, 48 accelerator_view and, 47, 64 automatically synchronizing data on, 142 constants using, 48 creating views, 48–49 default, 206–207 efficient global memory access, 146–148 functions using, 48 in GPU vs. accelerator memory in array, 64 REF, 205 setting for running parallel_for_each(), 106 supporting double precision support, 162 WARP, 205, 218, 220 accelerators, working with multiple about, 203 braided parallelism, 219 choosing about, 203 default accelerator, 206–207 enumerating accelerators, 203 dynamic load balancing, 216–218 falling back to CPU, 220–221 in Cartoonizer case study forked pipeline strategy, 249–252 strategy for, 246–248 swapping data between accelerators, 211–214 using more than one GPU, 208–211, 212 accelerator_view accelerator and, 48 arrays bounded to, 46 default accelerator and, 207 Direct3D device interop and, 276–277 in arrays, 47 omitting setting code for debugging, 108–109
Psychological Capital of MIS Development Teams, System Effectiveness, and Social Innovation: A Systematic Literature Review

Inter-organizational information systems are critical in the modern business world, as a large portion of economic activities are done through inter-organizational collaborations. One such important collaboration is social innovation/entrepreneurship, which involves multiple parties from diverse areas cooperating in major tasks. Against such a backdrop, exploring beneficial factors for organizational members to actively develop and/or make good use of a management information system (MIS) for collaborative performance has become a vital research question. This conceptual paper argues that positive psychology is critical to encourage members to volunteer to construct an MIS that facilitates social innovation effectiveness. Specifically, we discuss the four dimensions of psychological capital (i.e., hope, optimism, self-efficacy, and resilience) on effective inter-organizational MIS attributes (e.g., connectivity), and on the consequences of social innovation. At the core of this discussion, we believe that a positive psychological foundation is a driver for staff effort to contribute to a better MIS, which could benefit social innovation success. This research contributes by offering inspiration for future studies to link the micro- and macro-level aspects of social innovation and entrepreneurship phenomena.