Miracle-dz/UniFAR-FTUs huggingface dataset:
Energy Intake and Growth of 3- to 36-Month-Old German Infants and Children

Recently, new estimations of the energy requirements of infants and children were proposed as a basis of new FAO/WHO energy requirements. We have compared the energy intake of 354 healthy, well-nourished infants taking part in the Dortmund Nutritional and Anthropometric Longitudinal Designed Study (3-day-weighted diet records) with these new estimated energy requirements. The energy intake of breast-fed and formula-fed infants and the energy intake of the 1- to 3-year-old children in the study population corresponded well with the new estimated energy requirements, although these were considerably lower than the 1985 FAO/WHO energy requirements. Breast-feeding rates were lower and the introduction of beikost was earlier than the current recommendations. Height and weight, which were taken as global indicators of the adequacy of the energy intake, were in good accordance with The Netherlands’ third nationwide survey. Our data indicate that the new estimations of energy requirement seem to be more appropriate than the 1985 FAO/WHO energy requirements.
The effect on emergency department visits of raised alcohol minimum prices in Saskatchewan, Canada

INTRODUCTION AND AIMS The province of Saskatchewan, Canada introduced minimum prices graded by alcohol strength in April 2010. As previous research found this intervention significantly decreased alcohol consumption and alcohol-attributable morbidity, we aim to test the association between the intervention and the rate of emergency department (ED) visits in four alcohol-related injury categories [motor vehicle collisions (MVC), assaults, falls and total alcohol-related injuries]. DESIGN AND METHODS Data on ED visits in the city of Regina were obtained from the Saskatchewan Ministry of Health. Auto-regressive integrated moving average time series models were used to test the immediate and lagged effects of the pricing intervention on rates of alcohol-related nighttime. ED visits and controlled for daytime rates of ED visits, economic variables, linear and seasonal trends, and auto-regressive and moving average effects. RESULTS The implementation of an alcohol minimum pricing strategy in Saskatchewan was associated with decreased MVC-related ED visits for women aged 26 and over after a 6 month lag period (-39.4%, P < 0.001). There was no significant abrupt effect of this intervention on ED visits of four injury types in any of four gender-age categories; however, rates of ED visits among young males for MVCs and assaults decreased substantially during this study. DISCUSSION AND CONCLUSIONS The minimum pricing policy change led to a lagged decrease in motor vehicle-collision-related ED visits for women older than 25. Of note, there did not appear to be an instantaneous effect on the rate of alcohol-related injury ED visits immediately after the policy implementation nor lagged effects for other gender-age groups.
Colorectal Cancer Consensus Molecular Subtypes Translated to Preclinical Models Uncover Potentially Targetable Cancer Cell Dependencies

Purpose: Response to standard oncologic treatment is limited in colorectal cancer. The gene expression–based consensus molecular subtypes (CMS) provide a new paradigm for stratified treatment and drug repurposing; however, drug discovery is currently limited by the lack of translation of CMS to preclinical models. Experimental Design: We analyzed CMS in primary colorectal cancers, cell lines, and patient-derived xenografts (PDX). For classification of preclinical models, we developed an optimized classifier enriched for cancer cell–intrinsic gene expression signals, and performed high-throughput in vitro drug screening (n = 459 drugs) to analyze subtype-specific drug sensitivities. Results: The distinct molecular and clinicopathologic characteristics of each CMS group were validated in a single-hospital series of 409 primary colorectal cancers. The new, cancer cell–adapted classifier was found to perform well in primary tumors, and applied to a panel of 148 cell lines and 32 PDXs, these colorectal cancer models were shown to recapitulate the biology of the CMS groups. Drug screening of 33 cell lines demonstrated subtype-dependent response profiles, confirming strong response to EGFR and HER2 inhibitors in the CMS2 epithelial/canonical group, and revealing strong sensitivity to HSP90 inhibitors in cells with the CMS1 microsatellite instability/immune and CMS4 mesenchymal phenotypes. This association was validated in vitro in additional CMS-predicted cell lines. Combination treatment with 5-fluorouracil and luminespib showed potential to alleviate chemoresistance in a CMS4 PDX model, an effect not seen in a chemosensitive CMS2 PDX model. Conclusions: We provide translation of CMS classification to preclinical models and uncover a potential for targeted treatment repurposing in the chemoresistant CMS4 group. Clin Cancer Res; 24(4); 794–806. ©2017 AACR.
O Discurso, a Análise de Discurso e a Metodologia do Discurso do Sujeito Coletivo na Gestão Intercultural

O objetivo deste artigo e estabelecer um dialogo entre o discurso, a analise de discurso e a metodologia do Discurso do Sujeito Coletivo desenvolvida por Lefevre e Lefevre, e exemplificar sua aplicacao em uma pesquisa de gestao intercultural. Inicialmente, discutem-se os conceitos de discurso e de analise de discurso, relacionando-os a metodologia de analise do Discurso do Sujeito Coletivo. Em uma secao em separado, discorre-se criticamente sobre a metodologia do DSC e sua aplicacao em uma pesquisa com gestores interculturais. As consideracoes finais apontam as semelhancas e especificidades do DSC em relacao a analise do discurso tradicional e suas principais contribuicoes.
Comparing auditory, visual and vibrotactile cues in individuals with Parkinson’s disease for reducing risk of falling over different types of soil

Abstract Introduction Several researchers have demonstrated the positive benefits of auditory and visual cueing in the gait improvements among individuals with Parkinson’s disease (PD). However, few studies have evaluated the role of vibrotactile cueing when compared to auditory and visual cueing. This paper compares how these stimuli affect the risk of falling while walking on six types of soil (concrete, sand, parquet, broken stone, two types of carpet). Methods An instrumented Timed Up and Go (iTUG) test served to evaluate how audio, visual and vibrotactile cueing can affect the risk of falling of elderly. This pilot study proposes 12 participants with PD (67.7 ± 10.07 years) and nine age-matched controls (66.8 ± 8.0 years). Both groups performed the iTUG test with and without cueing. The cueing frequency was set at 10% above the cadence computed at the lower risk level of falling (walking over the concrete). A computed risk of falling (ROFA) index has been compared to the TUG time (total TUG duration). Results The index for evaluating the risk of falling appears to have a good reliability (ICC > 0.88) in this pilot study. In addition, the minimal detectable change (MDC) suggests that the proposed index could be more sensitive to the risk of falling variation compared to the TUG time. Moreover, while using the cueing, observed results suggest a significant decrease in the computed risk of falling compared to ‘without cueing’ for most of types of soil, especially for deformable soils, which can lead to falls. Conclusion When compared to other cueing, it seems that audio could be a better neurofeedback for reducing the risk of falling over different walking surfaces, which represent important risk factors for persons with gait disorder or lost functional autonomy.
Fast and Slow Oscillations Recruit Molecularly-Distinct Subnetworks of Lateral Hypothalamic Neurons In Situ

Abstract Electrical signals generated by molecularly-distinct classes of lateral hypothalamus (LH) neurons have distinct physiological consequences. For example, LH orexin neurons promote net body energy expenditure, while LH non-orexin neurons [VGAT, melanin-concentrating hormone (MCH)] drive net energy conservation. Appropriate switching between such physiologically-opposing LH outputs is traditionally thought to require cell-type-specific chemical modulation of LH firing. However, it was recently found that, in vivo, the LH neurons are also physiologically exposed to electrical oscillations of different frequency bands. The role of the different physiological oscillation frequencies in firing of orexin vs non-orexin LH neurons remains unknown. Here, we used brain-slice whole-cell patch-clamp technology to target precisely-defined oscillation waveforms to individual molecularly-defined classes LH cells (orexin, VGAT, MCH, GAD65), while measuring the action potential output of the cells. By modulating the frequency of sinusoidal oscillatory input, we found that high-frequency oscillations (γ, ≈30–200 Hz) preferentially silenced the action potential output orexinLH cells. In contrast, low frequencies (δ-θ, ≈0.5–7 Hz) similarly permitted outputs from different LH cell types. This differential control of orexin and non-orexin cells by oscillation frequency was mediated by cell-specific, impedance-unrelated resonance mechanisms. These results substantiate electrical oscillations as a novel input modality for cell-type-specific control of LH firing, which offers an unforeseen way to control specific cell ensembles within this highly heterogeneous neuronal cluster.
DFT Study on Intermetallic Pd–Cu Alloy with Cover Layer Pd as Efficient Catalyst for Oxygen Reduction Reaction

Detailed density functional theory (DFT) calculations of the adsorption energies (Ead) for oxygen on monolayer Pd on top of the Pd–Cu face-centered cubic (FCC) alloy and intermetallic B2 structure revealed a linear correspondence between the adsorption energies and the d-band center position. The calculated barrier (Ebarrier) for oxygen dissociation depends linearly on the reaction energy difference (ΔE). The O2 has a stronger adsorption strength and smaller barrier on the intermetallic Pd–Cu surface than on its FCC alloy surface. The room-temperature free energy (ΔG) analysis suggests the oxygen reduction reaction (ORR) pathways proceed by a direct dissociation mechanism instead of hydrogenation into OOH. These results might be of use in designing intermetallic Pd–Cu as ORR electrocatalysts.
Structure-sensitive enantiospecific adsorption on naturally chiral Cu(hkl)R&S surfaces

The desorption kinetics of a chiral compound, R-3-methylcyclohexanone (R-3MCHO), have been measured on both enantiomers of seven chiral Cu(hkl)R&S surfaces and on nine achiral Cu single crystal surfaces with surface structures that collectively span the various regions of the stereographic triangle. The naturally chiral surfaces have terrace-step-kink structures formed by all six possible combinations of the three low Miller index microfacets. The chirality of the kink sites is defined by the rotational orientation of the (1 1 1), (1 0 0) and (1 1 0) microfacets forming the kink. R-3MCHO adsorbs reversibly on these Cu surfaces and temperature programmed desorption has been used to measure its desorption energetics from the chiral kink sites. The desorption energies from the R- and S-kink sites are enantiospecific, ΔΔEdesS−R=ΔEdesS−ΔEdesR≠0, on the chiral surfaces. The magnitude of the enantiospecificity is |ΔΔEdesS−R| ≈ 1 kJ mol−1 on all seven chiral surfaces. However, the values of ΔΔEdesS−R are sensitive to elements of the surface structure other than just their sense of chirality as defined by the rotational orientation of the low Miller index microfacets forming the kinks; ΔΔEdesS−R changes sign within the set of surfaces of a given chirality.
Stem cell and skin rejuvenation

ABSTRACT Stem cell-based therapies have been widely used for their abilities to repair and regenerate different types of tissues and organs in cosmetic and plastic surgeries. It involves the clinical application of different types of stem cells. Different stem cells have been reported to be applicable in different areas of cosmetic surgeries like face lipoatrophy, skin rejuvenation, breast enhancement, and body contouring. However, adipose-derived stem cells remain the most widely used by cosmetic surgeons as they have the potential and capability to differentiate into mesenchymal, ectodermal, and endodermal lineages and are easily accessible to harvest. The purpose of this review is to summarize available applications of stem in cosmetic and plastic surgeries.
Community Paramedicine Applied in a Rural Community

RESEARCH OBJECTIVE Abbeville County Emergency Management Services (ACEMS) began a community paramedicine (CP) program to utilize trained paramedics to serve patients who frequently use the emergency department (ED) and have 1 or more of the following diagnoses: hypertension, diabetes, chronic heart failure, asthma, and chronic obstructive pulmonary disease. The objective of this study was to determine if the CP program reduced ED visits in Abbeville while improving patient outcomes. DESIGN A pre/posttest with a comparison group study design was used to evaluate the CP program. The study population had 193 patients (68 enrollees and 125 comparisons) who resided in Abbeville County, South Carolina. Frequent users of the ED were recruited and enrolled in the program by Abbeville Area Medical Center (AAMC) staff starting in October 2013. Records from both AAMC and ACEMS were examined to determine the impact of the CP program. RESULTS Hypertensive patients decreased an average of 7.2 mmHg (P < .0001) in systolic blood pressure and 4.0 mmHg (p < .0001) in diastolic blood pressure. Diabetic patients decreased blood glucose by an average of 33.7 mmol/L (p = .0013). Following enrollment into the program, CP participants decreased ED visits by 58.7% and inpatient visits by 68.8%. Conversely, the comparison group increased ED visits by 4.0% and inpatient visits by 187.5%. CONCLUSIONS The CP program demonstrated a meaningful difference in the health of participants while reducing their health care utilization. CP patients reduced their ED and inpatient use, required less intensive care, had better health outcomes, and reduced health expenses to the community.
Specialty Training’s Organizational Readiness for curriculum Change (STORC): validation of a questionnaire

Background The field of postgraduate medical education (PGME) is continuously evolving as a result of social demands and advancing educational insights. Change experts contend that organizational readiness for change (ORC) is a critical precursor for successful implementation of change initiatives. However, in PGME, assessing change readiness is rarely considered while it could be of great value for managing educational change such as curriculum change. Therefore, in a previous Delphi study the authors developed an instrument for assessing ORC in PGME: Specialty Training’s Organizational Readiness for curriculum Change (STORC). In this study, the psychometric properties of this questionnaire were further explored. Methods In 2015, STORC was distributed among clinical teaching teams in the Netherlands. The authors conducted a confirmatory factor analysis on the internal factor structure of STORC. The reliability of the measurements was estimated by calculating Cronbach’s alpha for all sub-scales. Additionally, a behavioral support-for-change measure was distributed as well to assess correlations with change-related behavior. Results In total, the STORC questionnaire was completed by 856 clinical teaching team members from 39 specialties. Factor analysis led to the removal of 1 item but supported the expected factor structure with very good fit for the other 43 items. Supportive behavior was positively correlated to a higher level of ORC. Discussion In this study, additional steps to collect validity evidence for the STORC questionnaire were taken successfully. The final subscales of STORC represent the core components of ORC in the literature. By breaking down this concept into multiple measurable aspects, STORC could help to enable educational leaders to diagnose possible hurdles in implementation processes and to perform specifically targeted interventions when needed.
Molecular regulation of ERK5 in development of diabetic retinopathy

Diabetic retinopathy (DR) is a major complication of diabetes, and causes pathological changes in retina blood vessels, as the most common cause of vision loss. Extracellular-signal-regulated kinase 5 (ERK5) is the newest discovered member in the mitogen-activated protein kinases (MAPKs) family, and recent evidence demonstrates an essential role of ERK5 signaling in the angiogenesis. However, whether ERK5 signaling may regulate DR development is unknown. Here, we used a streptozocin (STZ)-induce mouse DR model to investigate this question. We detected significant increases in the phosphorylation of ERK5, a signature of ERK5 activation in the purified retinal endothelial cells in DR mice, compared to control mice. In vivo suppression of ERK5 phosphorylation through administration of a specific inhibitor of ERK5 activation, BIX02189, did not prevent the occurrence of STZ-induced diabetes in mice, but significantly alleviated the severity of DR, seemingly through attenuating the retina neovascularization. Thus, our study suggests a previously unappreciated role of ERK5 signaling in DR development.
Frequency-invariant differential microphone array design in the STFT domain

Differential microphone array (DMA) designed in the STFT domain has attracted many researches efforts recently for its flexibility and small size. Theoretically, a DMA can achieve a frequency invariant beampattern that is very helpful for many applications. But in practice, the mismatch between the designed beampattern and the ideal DMA pattern is out of control. In this paper, we propose a new measure on the beampattern misalignment, and deduce a convex solution based on constrained least square (CLS) and second-order cone (SOC) optimization. It is verified that the CLS method can provide almost ideal DMA pattern with controllable white noise gain (WNG). We also show that with more microphones, the overall system performance can be further improved.
Autonomous Pathfinding in Simulated 3D Environment

This paper presents an approach for navigating an autonomous agent in a simulated room environment with multiple obstacles. It presents a technique for depth map estimation using stereoscopic images - a vital intermediate technique for autonomous navigation and pathfinding. It also proposes a path planner interfaced with the depth map for locally planning a sequence of steps to the desired location. A single RGB camera does not contain sufficient information to estimate depth with satisfactory accuracy. Depth cameras are significantly more expensive than RGB cameras, and hence would not be suited for projects requiring lower budget constraints. Hence a middle ground is required which requires depth calculation from limited available input. Depth estimation is accomplished with a convolutional neural network trained on synthetic randomly generated stereo image data. The path planner comprises a short-term path planner for obstacle avoidance, and a long-term path planner for approaching the destination co-ordinates. The results show that this approach yields a factor of approximately 1.8 times the steps of human performance on the same task.
Regulator of G protein signaling-12 modulates the dopamine transporter in ventral striatum and locomotor responses to psychostimulants

Regulators of G protein signaling are proteins that accelerate the termination of effector stimulation after G protein-coupled receptor activation. Many regulators of G protein signaling proteins are highly expressed in the brain and therefore considered potential drug discovery targets for central nervous system pathologies; for example, here we show that RGS12 is highly expressed in microdissected mouse ventral striatum. Given a role for the ventral striatum in psychostimulant-induced locomotor activity, we tested whether Rgs12 genetic ablation affected behavioral responses to amphetamine and cocaine. RGS12 loss significantly decreased hyperlocomotion to lower doses of both amphetamine and cocaine; however, other outcomes of administration (sensitization and conditioned place preference) were unaffected, suggesting that RGS12 does not function in support of the rewarding properties of these psychostimulants. To test whether observed response changes upon RGS12 loss were caused by changes to dopamine transporter expression and/or function, we prepared crude membranes from the brains of wild-type and RGS12-null mice and measured dopamine transporter-selective [3H]WIN 35428 binding, revealing an increase in dopamine transporter levels in the ventral–but not dorsal–striatum of RGS12-null mice. To address dopamine transporter function, we prepared striatal synaptosomes and measured [3H]dopamine uptake. Consistent with increased [3H]WIN 35428 binding, dopamine transporter-specific [3H]dopamine uptake in RGS12-null ventral striatal synaptosomes was found to be increased. Decreased amphetamine-induced locomotor activity and increased [3H]WIN 35428 binding were recapitulated with an independent RGS12-null mouse strain. Thus, we propose that RGS12 regulates dopamine transporter expression and function in the ventral striatum, affecting amphetamine- and cocaine-induced increases in dopamine levels that specifically elicit acute hyperlocomotor responses.
Afferent and Efferent Connections of Nucleus praeeminentialis in the Channel Catfish: A Reevaluation

Nucleus praeeminentialis (nPr) is an isthmic nucleus that has been described in the brains of electrosensory teleost fishes and a single non-electrosensory species. The nucleus receives axon collaterals of ascending medullary sensory lemniscal neurons. Axons of nPr neurons project in turn back down onto those same populations of medullary projection neurons via a descending parallel fiber system (the molecular layer or cerebellar crest). Thus nPr forms a link in a sensory feedback loop that modulates the activity of neurons that relay information from medulla to midbrain. The purpose of this study is to investigate the nature of the afferent and efferent connections of the nPr with the specific aim of investigating other sources of input into this modulatory circuit. Transport of neuronal tracers (horseradish peroxidase, DiI and dextran amines) revealed that nPr has extensive interconnections with nuclei in the basal metencephalon, cerebellum, octavolateralis column and basal medulla. A previously described source of afference, the torus semicircularis in the mesencephalon, was not indicated by our studies. Our studies suggest that in addition to regulating the sensitivity and resolution of electrosensory and mechanosensory lateral line systems, the nPr may play a role in the resolution of signal ambiguities posed by auditory or vestibular stimulation of the saccular endorgan of the inner ear.
Macondo‐1 well oil‐derived polycyclic aromatic hydrocarbons in mesozooplankton from the northern Gulf of Mexico

Mesozooplankton (>200 μm) collected in August and September of 2010 from the northern Gulf of Mexico show evidence of exposure to polycyclic aromatic hydrocarbons (PAHs). Multivariate statistical analysis revealed that distributions of PAHs extracted from mesozooplankton were related to the oil released from the ruptured British Petroleum Macondo‐1 (M‐1) well associated with the R/VDeepwater Horizon blowout. Mesozooplankton contained 0.03–97.9 ng g−1 of total PAHs and ratios of fluoranthene to fluoranthene + pyrene less than 0.44, indicating a liquid fossil fuel source. The distribution of PAHs isolated from mesozooplankton extracted in this study shows that the 2010 Deepwater Horizon spill may have contributed to contamination in the northern Gulf of Mexico ecosystem.