Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterisation of Drug Delivery Efficacy Using Microstructure-Assisted Application of a Range of APIs

Polymer-based solid microstructures (MSts) have the potential to significantly increase the quantity and range of drugs that can be administered across the skin. MSt arrays are used to demonstrate their capacity to bypass the skin barrier and enhance permeability by creating microchannels through the stratum corneum, in a minimally invasive manner. This study is designed to demonstrate the ability of MSts to exceed the current boundaries for transdermal delivery of compounds with different molecular weights, partition coefficients, acid dissociation constants, melting points, and water solubilities. In vitro permeation of a range of selected molecules, including acetyl salicylic acid (aspirin), galantamine, selegiline hydrochloride (Sel-HCl), insulin, caffeine, hydrocortisone (HC), hydrocortisone 21-hemisuccinate sodium salt (HC-HS) and bovine serum albumin (BSA) has been studied across excised porcine skin with and without poke and patch application of MSts. Permeation of the molecules was monitored using Franz diffusion cells over 24 h. MSts significantly increased the permeation of all selected molecules up to 40 times, compared to topical applications of the molecules without MSts. The greatest increase in permeation was observed for caffeine with 70 ± 8% permeation and the lowest enhancement was observed for HC with a 2.4 ± 1.3% increase in permeation. The highest obtained flux was BSA (8133 ± 1365 μg/cm2/h) and the lowest flux observed for HC (11 ± 4 μg/cm2/h). BSA and HC also showed the highest (16,275 ± 3078 μg) and the lowest (73 ± 47 μg) permeation amount after 24 h respectively. MSt-treated skin exhibits greatly increased permeation. The molecule parameters (size, acid dissociation constant, partition coefficient and solubility)—traditional hurdles associated with passive diffusion through intact skin—are overcome using MSt skin treatment.
The Effects of Grain Boundary Misorientation on the Mechanical Properties and Mechanism of Plastic Deformation of Ni/Ni3Al: A Molecular Dynamics Study

The effects of grain boundary misorientation angle (θ) on mechanical properties and the mechanism of plastic deformation of the Ni/Ni3Al interface under tensile loading were investigated using molecular dynamics simulations. The results show that the space lattice arrangement at the interface is dependent on grain boundary misorientations, while the interfacial energy is dependent on the arrangement. The interfacial energy varies in a W pattern as the grain boundary misorientation increases from 0° to 90°. Specifically, the interfacial energy first decreases and then increases in both segments of 0–60° and 60–90°. The yield strength, elastic modulus, and mean flow stress decrease as the interfacial energy increases. The mechanism of plastic deformation varies as the grain boundary misorientation angle (θ) increases from 0° to 90°. When θ = 0°, the microscopic plastic deformation mechanisms of the Ni and Ni3Al layers are both dominated by stacking faults induced by Shockley dislocations. When θ = 30°, 60°, and 80°, the mechanisms of plastic deformation of the Ni and Ni3Al layers are the decomposition of stacking faults into twin grain boundaries caused by extended dislocations and the proliferation of stacking faults, respectively. When θ = 90°, the mechanisms of plastic deformation of both the Ni and Ni3Al layers are dominated by twinning area growth resulting from extended dislocations.
Pentafluoro‐3‐hydroxy‐pent‐2‐en‐1‐ones Potently Inhibit FNT‐Type Lactate Transporters from all Five Human‐Pathogenic Plasmodium Species

The protozoan parasite Plasmodium falciparum causes the most severe and prevailing form of malaria in sub‐Saharan Africa. Previously, we identified the plasmodial lactate transporter, PfFNT, a member of the microbial formate–nitrite transporter family, as a novel antimalarial drug target. With the pentafluoro‐3‐hydroxy‐pent‐2‐en‐1‐ones, we discovered PfFNT inhibitors that potently kill P. falciparum parasites in vitro. Four additional human‐pathogenic Plasmodium species require attention, that is, P. vivax, most prevalent outside of Africa, and the regional P. malariae, P. ovale and P. knowlesi. Herein, we show that the plasmodial FNT variants are highly similar in terms of protein sequence and functionality. The FNTs from all human‐pathogenic plasmodia and the rodent malaria parasite were efficiently inhibited by pentafluoro‐3‐hydroxy‐pent‐2‐en‐1‐ones. We further established a phenotypic yeast‐based FNT inhibitor screen, and found very low compound cytotoxicity and monocarboxylate transporter 1 off‐target activity on human cells, particularly of the most potent FNT inhibitor BH267.meta, allowing these compounds to proceed towards animal model malaria studies.
Fatty Acid Profile and Enterolactone Content of Early and Commercial Milk of Dairy Cows Supplemented with Flaked Flaxseed during the Dry Period

Simple Summary Several studies have been published on n-3 fatty acids enrichment of food of animal origin by the use of specific supplements in animal feeding. Flaxseed (Linum usitatissimum) is known as an excellent source of unsaturated fatty acids, particularly of alpha-linolenic acid. Moreover, most of the plant lignans contained in flaxseeds are converted by the animal in mammalian lignans, such as enterodiol and enterolactone. Thus, milk with elevated enterolactone may be an efficient strategy to optimize the effects of lignans on human health. This study aimed to investigate the effect of flacked flaxseed supplementation on dairy cow during the pre-partum period. The increase of n-3 on milk obtained from cow fed the flaxseed diet was detected only in the first days of lactation. In addition, an increase of enterolactone on milk from flaxseed fed cows was observed only at 15th day after calving; a higher amount of enetrolactone was detected in plasma and milk from the 15th day after calving, independently from the treatment group. This study suggests that the duration of n-3 carry-over into the milk is dependent on the n-3 feeding source concentration. Finally, more studies are needed to investigate enterolactone metabolism in ruminants. Abstract Various supplementations in animal feeding have been investigate in order to enrich food of animal origin with n-3 fatty acids. Although the effects of flaxseeds inclusion in diets for lactating dairy have already been assessed, few studies have focused on this n-3 source supplementation during the transition period. The aim of this work was to evaluate the effects of flacked flaxseed (200 g/head/day; 2.13% DM) dietary treatment during the dry period on milk yield and quality in the 30 days after calving. In addition, the enterolactone content in plasma (before and after calving) and in milk of cows fed diets supplemented or not with flaxseed was considered. The study demonstrated that the carry-over effect on the milk profile of C18:2, C18:3 n-3, and C20:5 n-3 was significantly higher in flaxseed diet than in the control one at 4th day of lactation. A significant increase of enterolactone on milk from flaxseed fed cows was observed only at 15 sampling day. The quick modification in fatty acid (FA) profile of the milk in the first few days of lactation suggests that the carry over effect from pre-calving flaxseed feeding at this concentration was very short lasting.
Cellular, Extracellular and Extracellular Vesicular miRNA Profiles of Pre-Ovulatory Follicles Indicate Signaling Disturbances in Polycystic Ovaries

Cell-free RNAs have the potential to act as a means of gene expression regulation between cells and are therefore used as diagnostic markers describing the state of tissue environment. The origin and functions of such RNAs in human ovarian follicle, the environment of oocyte maturation, are unclear. The current study investigates the difference in the microRNA profiles of fertile women and polycystic ovary syndrome (PCOS) patients in three compartments from the same preovulatory follicle: mural granulosa cells (MGC), cell-free follicular fluid (FF), and extracellular vesicles (EV) of the FF by small RNA sequencing. In silico analysis was used for the prediction and over-representation of targeted pathways for the detected microRNAs. PCOS follicles were distinguished from normal tissue by the differential expression of 30 microRNAs in MGC and 10 microRNAs in FF (FDR < 0.1) that commonly regulate cytokine signaling pathways. The concentration of EV-s was higher in the FF of PCOS patients (p = 0.04) containing eight differentially expressed microRNAs (p < 0.05). In addition, we present the microRNA profiles of MGC, FF, and EV in the fertile follicle and demonstrate that microRNAs loaded into EVs target mRNAs of distinct signaling pathways in comparison to microRNAs in FF. To conclude, the three follicular compartments play distinct roles in the signaling disturbances associated with PCOS.
Hand Function in 8- to 12-Year-Old Children with Bilateral Cerebral Palsy and Interpretability of the Both Hands Assessment

Abstract Aim To describe bimanual performance in a sample of Australian children with bilateral cerebral palsy (CP) and, examine the qualitative meaning (or interpretability) of scores on the Both Hands Assessment (BoHA). Methods Children with bilateral CP aged 8–12 years (n = 54) classified Manual Ability Classification System (MACS) level I = 20, II = 18, III = 16 were examined using the BoHA. Results Bimanual performance was significantly different across MACS levels I-III (p < 0.001). Mean (95%CI) BoHA-unit for each MACS level were I = 85 (81–89), II = 72 (68–76) and III = 53 (49–56). Children with asymmetrical hand use (≥ 20% difference between upper limbs, n = 10) were classified MACS levels II and III and had a mean (95%CI) BoHA-unit of 56 (51–62). Children with symmetrical hand use were classified in MACS level I-III and had a mean (95%CI) BoHA-unit of 74 (70–79). Conclusions The BoHA quantified observations of bimanual performance for children with bilateral CP, differentiated between MACS levels I-III and provided clinically meaningful information. The BoHA may facilitate tailoring of upper limb intervention. Future research is recommended to examine inter-rater and intra-rater reliability and responsiveness of the BoHA, as well as longitudinal studies of bimanual hand skill development in children with bilateral CP.
Knowledge about the nature of science increases public acceptance of science regardless of identity factors

While people’s views about science are related to identity factors (e.g. political orientation) and to knowledge of scientific theories, knowledge about how science works in general also plays an important role. To test this claim, we administered two detailed assessments about the practices of science to a demographically representative sample of the US public (N = 1500), along with questions about the acceptance of evolution, climate change, and vaccines. Participants’ political and religious views predicted their acceptance of scientific claims, as in prior work. But a greater knowledge of the nature of science and a more mature view of how to mitigate scientific disagreements each related positively to acceptance. Importantly, the positive effect of scientific thinking on acceptance held regardless of participants’ political ideology or religiosity. Increased attention to developing people’s knowledge of how science works could thus help to combat resistance to scientific claims across the political and religious spectrum.
Biotechnological Approaches on Two High CBD and CBG Cannabis sativa L. (Cannabaceae) Varieties: In Vitro Regeneration and Phytochemical Consistency Evaluation of Micropropagated Plants Using Quantitative 1H-NMR

High cannabidiol (CBD) and cannabigerol (CBG) varieties of Cannabis sativa L. , a species with medicinal properties, were regenerated in vitro. Explants of nodal segments including healthy axillary bud, after sterilization, were placed in Murashige-Skoog (MS) culture medium. The shoots formed after 30 days were subcultured in full- or half-strength MS medium supplemented with several concentrations of 6-benzyl-amino-purine (BA) or thidiazuron (TDZ). The highest average number and length of shoots was achieved when both full and half-strength MS media were supplemented with 4.0 μM BA. The presence of 4.0 μM TDZ showed also comparable results. BA and TDZ at concentrations of 4.0, 8.0 μM and 2.0, 4.0 μM respectively, displayed the maximum shooting frequency. The new shoots were transferred on the same media and were either self-rooted or after being enhanced with different concentrations of indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA). Presence of 2.0 or 4.0 μM IBA or 4.0 μM NAA resulted to the optimum rooting rates. The maximum average number and length of roots per shoot was observed when the culture media was supplemented with 4.0 μM IBA or NAA. Approximately 92% of the plantlets were successfully established and acclimatized in field. The consistency of the chemical profile of the acclimatized in vitro propagated clones was assessed using quantitative 1H-NMR high throughput screening. In each variety, analysis of the micropropagated plant in comparison with the mother plant showed no statistically significant differences (p ≤ 0.05) in CBD+ cannabidiolic acid (CBDA) and CBG+ cannabigerolic acid (CBGA) content respectively, thus indicating stability of their chemical profile.
Acute Compartment Syndrome of the Lower Leg Using Navigated Endoprosthesis of the Knee Akutes Kompartmentsyndrom des Unterschenkels nach navigierter Kniegelenksendoprothetik – ein Fallbericht

Abstract Acute compartment syndrome of the lower leg following computer navigated primary total knee arthroplasty is rare but potentially devastating. This could be triggered by a setting of the bicortical tibial navigation pin. It is essential to take care during the operation, and to implement close post-operative control, especially if there are risk factors or nerve blocks. Zusammenfassung Ein Kompartmentsyndrom des Unterschenkels bei navigierter Kniegelenksendoprothetik ist selten, es existieren nur vereinzelt Kasuistiken hierzu. Kommt es jedoch zu einem manifesten Kompartmentsyndrom, so ist dies eine schwerwiegende Komplikation mit teilweise verheerenden Verläufen. Vermutlicher Auslöser in unserem Fallbeispiel ist das traumatische Setzen eines bikortikalen tibialen Navigations-Pins. Die Sorgfältigkeit während der Operation, ebenso wie die engmaschige postoperative Kontrolle des Lokalbefundes, insbesondere beim Vorliegen von Risikofaktoren oder additiv gesetzten Regionalanästhesien, die das klinische Bild verschleiern können, sind essenziell und müssen dem Operateur sowie dem betreuenden Ärzte- und Pflegeteam stets bewusst sein.
Validation of the Five-Factor Obsessive–Compulsive Inventory in a Mental Health Sample

The Five-Factor Obsessive–Compulsive Inventory (FFOCI) is a recently developed measure of obsessive–compulsive personality disorder (OCPD) based on an established dimensional model of general personality, the five-factor model. The present study aimed to evaluate the construct validity of the FFOCI by examining its associations with both traditional and dimensional diagnostic models of OCPD. This study used an archival data set of mental health patients that employed a multimethod design. A total of 214 individuals (and their nominated informants) completed various personality inventories indexing the Diagnostic and Statistical Manual for Mental Disorders–Fifth edition traditional and Alternative Model of Personality Disorders (AMPD) diagnostic criteria for OCPD, as well as other individual AMPD personality traits deemed conceptually relevant to the FFOCI. The results were generally quite supportive of construct validity. They showed that FFOCI scale scores converged with traditional measures of OCPD and AMPD–OCPD traits and impairment in a conceptually expected manner, with a few exceptions. Overall, it was clear from these findings that the FFOCI takes a broader and more comprehensive approach to the assessment of obsessive–compulsive than traditional Diagnostic and Statistical Manual and AMPD operationalizations.
Firefighters Are More Physically Active On-Duty Compared to Off-Duty

Physical inactivity, coupled with increasing obesity levels, in firefighters plays a key role in aggregated cardiovascular events. The purpose of this study was to investigate device-measured physical activity (PA) for firefighters while on- and off-duty to have a clearer understanding of their overall PA level. Methods: Twenty-nine career firefighters participated in this non-experimental, within-subjects study by wearing an accelerometer to assess PA intensities and step-count. Obesity was classified using body mass index (BMI). Dependent t-tests were used to examine mean differences in PA intensities when on- and off-duty. Pearson product-moment correlations were used to assess the association between PA intensities when on and off-duty. Results: According to the World Health Organization BMI categorizations, 20 firefighters were overweight, 9 were obese, and, thus, none were normal weight. Only light PA (LPA) was statistically significant (p = 0.026) for on- and off-duty days with a small-to-medium effect size (d = 0.47), meaning that on average, firefighters performed more minutes of LPA when on-duty compared to off. There was a significant difference between on- (9060.2 ± 2636.4) and off-duty (7495.3 ± 2835.8) daily step counts (p = 0.011). Conclusion: As the results demonstrate, there is a dire need for increased PA levels in firefighters while on- and off-duty.
Proteomic analysis of potato responding to the invasion of Ralstonia solanacearum UW551 and its type III secretion system mutant.

The infection of potato with Ralstonia solanacearum (R. solanacearum) UW551 gives rise to bacterial wilt disease via colonization of roots. The Type III Secretion System (T3SS) is a determinant factor for the pathogenicity of R. solanacearum. To fully understand perturbations in potato by R. solanacearum Type III Effectors(T3Es), we use proteomics to measure differences in potato root protein abundance after inoculation with R. solanacearum UW551 and T3SS mutant (UW551△HrcV). We identified 21 differentially accumulated proteins (DAPs). Compared to inoculation with UW551△HrcV, ten proteins showed significantly lower abundance in potato roots after inoculation with UW551, indicating those proteins were significantly down-regulated by T3Es during the invasion. To identify their functions in immunity, we silenced those genes in Nicotiana benthamiana and tested the resistance of the silenced plants to the pathogen. Results showed that miraculin, HBP2, TOM20 contribute to immunity to R. solanacearum. In contrast, PP1 contribute to susceptibility. Notably, none of four downregulated proteins (HBP2, PP1, HSP22, TOM20) were downregulated at the transcriptional level, suggesting that they were significantly down-regulated at the post-transcriptional level. We further co-expressed those four proteins with thirty-three core T3Es. To our surprise, multiple effectors were able to significantly decrease the sudied protein abundances. In conclusion, our data showed T3Es of R. solanacearum could subvert potato root immune-related proteins in a redundant manner.
Microalgae Encapsulation Systems for Food, Pharmaceutical and Cosmetics Applications

Microalgae are microorganisms with a singular biochemical composition, including several biologically active compounds with proven pharmacological activities, such as anticancer, antioxidant and anti-inflammatory activities, among others. These properties make microalgae an interesting natural resource to be used as a functional ingredient, as well as in the prevention and treatment of diseases, or cosmetic formulations. Nevertheless, natural bioactives often possess inherent chemical instability and/or poor solubility, which are usually associated with low bioavailability. As such, their industrial potential as a health-promoting substance might be severely compromised. In this context, encapsulation systems are considered as a promising and emerging strategy to overcome these shortcomings due to the presence of a surrounding protective layer. Diverse systems have already been reported in the literature for natural bioactives, where some of them have been successfully applied to microalgae compounds. Therefore, this review focuses on exploring encapsulation systems for microalgae biomass, their extracts, or purified bioactives for food, pharmaceutical, and cosmetic purposes. Moreover, this work also covers the most common encapsulation techniques and types of coating materials used, along with the main findings regarding the beneficial effects of these systems.
Rural Midwestern Mexican Fathers’ Perspectives on Family Food, Mealtimes, and Physical Activity

ABSTRACT Two focus group interviews with eight low-income Mexican fathers in two communities in a rural Midwestern state explored fathers’ perceptions, behaviors, and roles related to child and family food consumption, mealtimes, and physical activity. On average, fathers were 34 years of age, had 10 years of formal education, and annual household incomes ranged from $36,250 to $46,249. Findings include the identification of fifteen themes through the process of thematic analysis that are organized by five thematic areas of interest (family food patterns, strategies for healthy eating, family roles in food shopping and preparation, family mealtimes, physical activity among families). Findings revealed ways fathers contributed to their children’s and families’ health and well-being, and challenges they faced. Health promotion efforts aimed at rural, low-income Mexican immigrant families can benefit from embracing fathers’ perspectives and how they influence the health and well-being of their children and families.
Plasmonic Hybrids of MoS2 and 10-nm Nanogap Arrays for Photoluminescence Enhancement

Monolayer MoS2 has attracted tremendous interest, in recent years, due to its novel physical properties and applications in optoelectronic and photonic devices. However, the nature of the atomic-thin thickness of monolayer MoS2 limits its optical absorption and emission, thereby hindering its optoelectronic applications. Hybridizing MoS2 by plasmonic nanostructures is a critical route to enhance its photoluminescence. In this work, the hybrid nanostructure has been proposed by transferring the monolayer MoS2 onto the surface of 10-nm-wide gold nanogap arrays fabricated using the shadow deposition method. By taking advantage of the localized surface plasmon resonance arising in the nanogaps, a photoluminescence enhancement of ~20-fold was achieved through adjusting the length of nanogaps. Our results demonstrate the feasibility of a giant photoluminescence enhancement for this hybrid of MoS2/10-nm nanogap arrays, promising its further applications in photodetectors, sensors, and emitters.