Miracle-dz/UniFAR-FTUs huggingface dataset:
Pengaruh Estradiol-17? Dan Kolagen Tipe IV Terhadap Ekspresi Gen PIK3CA Untuk Menginduksi Ekspresi C-erbB2 Pada Lini Sel Kanker Ovarium SKOV-3

Ovarian cancer cell metastasis is induced by signaling pathway activated by the binding of type IV collagen to ? 1 integrin receptor on the surface of cancer cells and estrogen binding to estrogen receptor. However, the role of estradiol-17? and type IV collagen on the development of ovarian cancer have not been clearly understood. Therefore, this research was conducted to observe the differential gene expression in SKOV-3 ovarian cancer cells cultured on type IV collagen and treated with estradiol-17? , and incubated for 12, 24 and 72 hours. Differential display RT-PCR was used to express the differential expression gene after treatment. cDNA fragment that expressed differentially was isolated and sequenced. Sequencing result on one of the cDNA fragment showed that PI3K is one of the gene expressed in SKOV-3 ovarian cancer cell. To verify this result, cDNA was amplified using PIK3CA specific primer. The increasing level of PIK3CA is induced by three kinds of receptor activities, those are c-erbB2 receptor bound to estradiol-17? , homodimer receptor of c-erbB2, and the activity of integrin receptor bound to type IV collagen. The increasing level and activity of PIK3CA can also increase the expression of c-erbB2 gene. In SKOV-3 cells cultured on type IV collagen for 72 hours, the increasing of PIK3CA and c-erbB2 expression level is very low. The conclusion is that estradiol-17? gives a more significant effect than type IV collagen to induce the increasing expression of PIK3CA and c-erbB2 genes. Keywords : ovarian cancer, SKOV-3, type IV collagen, estradiol-17? , PIK3CA, c-erbB2
Molecular mapping of linked leaf rust resistance and non-glaucousness gene introgressed from Aegilops tauschii Coss. in hexaploid wheat Triticum aestivum L.

Abstract Aegilops tauschii, the D genome donor of wheat, is an invaluable source of genetic variability, which can be utilized for broadening the wheat gene pool. Linked leaf rust resistance and non-glaucousness genes transferred from Ae. tauschii to cultivated wheat variety WH542 were mapped in the present study. Genetic analysis in an F2 population from a BC3 plant derived from the cross Triticum durum cv. PBW114/Ae. tauschii acc. pau14195//4*T. aestivum cv. WH542 revealed monogenic dominant inheritance for both the traits. The leaf rust resistance and the non-glaucousness gene were tentatively named LrT and IwT, respectively. Leaf rust resistance gene exhibited all stage resistance. SSR markers Xbarc124, Xgdm5, Xgdm35, Xcfd51 and EST-derived markers Xcau96 and Xte6 on chromosome 2DS were linked with both genes. Chromosomal assignments of the genes were confirmed by testing linked SSR markers on Chinese Spring nulli-tetrasomics lines. SSR markers Xcau96 (1.6 cM) and Xbarc124 (0.6 cM) flanked LrT and Xgdm35 (4.1 cM) and Xte6 (2.5 cM) flanked non-glaucousness gene. LrT and IwT showed a recombination distance of 3.4 cM. Hence, IwT can be used as an easy to score morphological marker of LrT during its transfer to other glaucous backgrounds.
Cereal leaf beetles (Oulema spp., Coleoptera: Chrysomelidae) control following various dates of wheat sowing and insecticidal treatments

ABSTRACT In this paper, we report the sowing date and intensity of chemical control on presence of the cereal leaf beetles (CLB) on spring wheat. The CLB monitoring (Oulema melanopus L. and Oulema gallaeciana Voet.) was conducted in May–June of each year, following the announcement of the IOR-PiB in Poznań (Institute of Plant Protection, National Research Institute), that economic threshold levels in Poland of this pest is observed. For the spring sowing dates between (20 March–20 April) and late autumn sowings (21 November–7 December) larger amounts of CLB adults (1.6–1.1) in comparison to early autumn sowings (25 October–02 November) were noticed (0.4). Following the insecticidal seed treatment with imidacloprid, the occurrence of adults was 0.2–0.7 individual per stem, while the use of fungicidal treatment with triadimenol–imazalil–fuberidazol resulted in average 1.1–2.2 individual per stem. The reduction in the number of larvae per stem was 7–10 fold larger following the foliar application (pirimicarb + dimethoate + cypermethrin) compared to the seed treatment with imidacloprid. The lowest grain yield (3.83 t ha−1) from the spring crop without any insecticide was obtained. Foliar application with blend of pirimicarb + dimethoate + cypermethrin increased the yield crop by 0.41 t ha−1 (10.7%) and additionally by 0.78 t ha−1 (20.4%) to compare to the fungicidal control when imidacloprid was used. Wheat seed treatment with imidacloprid and sowing date in autumn showed to be suitable and economically efficient preventive strategies for controlling the pest population.
Optimization of Cultural and Physical Parameters for Phenol Biodegradation by Newly Identified Pseudomonas sp. AQ5-04

Phenol is widely used by many industries and it is one of the highly toxic environ - mental pollutants. Bioremoval is one of the most effective methods to remove phenol compared to other physio-chemical methods. Identification was carried out using 16s rRNA sequencing. Mineral salt media with 0.5 g/L phenol as the sole source of carbon. Factors influencing phenol degradation were optimised via one-factor-at-a-time and response surface methodology. Optimum degradation was achieved at pH 7.5, the temperature of 30°C and ammonium sulphate at 0.4 g/L. Using Response surface methodology the incubation period was reduced to 36 h compared to the OFAT approach where it takes 72 hours. The effect of 10 heavy metals at various concentrations was tested. The optimum values used for tempe - rature, pH, ammonium sulphate and salinity for both the OFAT and RSM have correlated with the only pH displayed the slighted difference of 7.0 for OFAT and 7.5 for RSM. This shows the closest optimum conditions for both methods. The strain is also resistance to some heavy metals usually found in polluted environ - ments together with phenol. Therefore, it can be clearly stated that Pseudomonas sp. strain AQ5-04 is the potential candidate for phenol bioremediation and further studies in the field of bioremediation. The bacterium can degrade phenol in the presence of between 1 to 3 ppm of the heavy metals As, Cd, Co, and Zn while growth and degradation were inhibited by Hg, Ag, Cu and Ni at 1 ppm. The isolate is a potential strain for further bioremediation studies.
Anterior Cingulate MicroRNA Expression Differences in Mood Disorder Patients: Contrasts with Transcriptomic Analyses in an Animal Model of Depression.

MicroRNAs (miRNAs) are small, non-coding RNAs acting as post-transcriptional regulators of gene expression. Though implicated in multiple CNS disorders, miRNAs have not been examined in any psychiatric disease state in anterior cingulate cortex (AnCg), a brain region centrally involved in regulating mood. We performed qPCR analyses of 29 miRNAs previously implicated in psychiatric illness (major depressive disorder (MDD), bipolar disorder (BP) and/or schizophrenia (SZ)) in AnCg of patients with MDD and BP versus controls. miR-132, miR-133a and miR-212 were differentially expressed in BP, miR-184 in MDD and miR-34a in both MDD and BP. In silico target prediction algorithms identified putative targets of differentially expressed miRNAs. Nuclear Co-Activator 1 (NCOA1), Nuclear Co-Repressor 2 (NCOR2) and Phosphodiesterase 4B (PDE4B) were selected based upon predicted targeting by miR34a (with NCOR2 and PDE4B both targeted by miR-184) and published relevance to psychiatric illness. Luciferase assays identified PDE4B as a target of miR-34a and miR-
Vitality and Growth of the Threatened Lichen Lobaria pulmonaria (L.) Hoffm. in Response to Logging and Implications for Its Conservation in Mediterranean Oak Forests

Forest logging can be detrimental for non-vascular epiphytes, determining the loss of key components for ecosystem functioning. Legal logging in a Mediterranean mixed oak forest (Tuscany, Central Italy) in 2016 heavily impacted sensitive non-vascular epiphytes, including a large population of the threatened forest lichen Lobaria pulmonaria (L.) Hoffm. This event offered the background for this experiment, where the potential effects of logging in oak forests are simulated by means of L. pulmonaria micro-transplants (thallus fragments <1 cm). Our working hypothesis is that forest logging could negatively influence the growth of the thalli exposed in logged stands compared to those exposed in unlogged stands. One hundred meristematic lobes and 100 non-meristematic fragments are exposed for one year on 20 Turkey oak trees (Quercus cerris), half in a logged and half in an unlogged stand. Chlorophyll (Chl) a fluorescence emission and total chlorophyll content are used as a proxy for the overall vitality of the transplants, while their growth is considered an indicator of long-term effects. Generally, vitality and growth of the transplants in the logged stand are lower than in the unlogged stand. Both vitality and growth vary between the meristematic and non-meristematic fragments, the former performing much better. Hence, irrespective of forest management, meristematic fragments show higher growth rates (0.16–0.18 cm2 year−1) than non-meristematic ones (0.02–0.06 cm2 year−1). Considering that a conservation-oriented management for this species should be tailored at the habitat-level and, especially, at the tree-level, our results suggest that for appropriate conservation strategies, it is necessary to consider the life cycle of the lichen, since the probability of survival of the species may vary, with meristematic fragments having more chance to survive after logging.
Excellent ONO passivation on phosphorus and boron diffusion demonstrating a 25% efficient IBC solar cell

This work presents results of a laboratory‐scale interdigitated back contact (IBC) solar cell with an independently measured efficiency of 25.0%, featuring open‐circuit voltage of 716 mV, short‐circuit current of 43.0 mA.cm−2 and fill factor of 81.0%. Notably, the high efficiency was achieved based on significant improvements resulting from the optimised cell structure, excellent SiO2‐SiNx‐SiOx (ONO) surface passivation, detailed bulk lifetime management strategy and improved random pyramid texturing. Experimental details and analysis of the individual improvements over prior work are presented in‐depth.
On the Production, Mating and Behavior of Males in Synoeca surinama (Vespidae: Polistinae: Epiponini)

In colonies of Neotropical swarm-founding wasps (Epiponini), males are thought to be produced only in periods when there are many queens present. Little information is available regarding male behavior in and out of the nest, and male mating strategies are poorly understood. Here, a behavioral study of males of Synoeca surinama is provided and copulation behavior inside a nest is described for the first time. A description of an aggregation of non-natal males at a nest is also provided. The behavior of males was observed in three colonies subjected to removal of queens for another study. Nest envelope was partially removed, and the observations were made directly (seen by “the naked eye”) and indirectly (through camcorder recordings), daily. Production of males occurred in large colonies with number of workers being more relevant than the number of the queens. Males of the aggregation were marked and filmed. The aggregation of non-natal males was observed for seven consecutive days next to the largest colony; some of the males attended the aggregation on consecutive days. The males were observed accessing the comb by the opening in the envelope and courting new queens. One successful copulation on the comb of the nest was observed. In the colony where the male aggregation was observed, 47.6% of the new queens were inseminated while in the other two colonies, no new queens were inseminated. These observations suggest males are attracted to, aggregate and seek mating opportunities at non-natal colonies that are producing young adult queens.
Temperature drives Zika virus transmission: evidence from empirical and mathematical models

Temperature is a strong driver of vector-borne disease transmission. Yet, for emerging arboviruses we lack fundamental knowledge on the relationship between transmission and temperature. Current models rely on the untested assumption that Zika virus responds similarly to dengue virus, potentially limiting our ability to accurately predict the spread of Zika. We conducted experiments to estimate the thermal performance of Zika virus (ZIKV) in field-derived Aedes aegypti across eight constant temperatures. We observed strong, unimodal effects of temperature on vector competence, extrinsic incubation period, and mosquito survival. We used thermal responses of these traits to update an existing temperature-dependent model to infer temperature effects on ZIKV transmission. ZIKV transmission was optimized at 29°C, and had a thermal range of 22.7°C - 34.7°C. Thus, as temperatures move toward the predicted thermal optimum (29°C) due to climate change, urbanization, or seasonally, Zika could expand north and into longer seasons. In contrast, areas that are near the thermal optimum were predicted to experience a decrease in overall environmental suitability. We also demonstrate that the predicted thermal minimum for Zika transmission is 5°C warmer than that of dengue, and current global estimates on the environmental suitability for Zika are greatly over-predicting its possible range.
Carbohydrate processing by bacterial pathogens: structural and functional analyses of glycoside hydrolases.

Supervisory Committee Dr. Alisdair B. Boraston (Department of Biochemistry and Microbiology) Supervisor Dr. Paul J. Romaniuk (Department of Biochemistry and Microbiology) Departmental Member Dr. Martin Boulanger (Department of Biochemistry and Microbiology) Departmental Member Dr. John S. Taylor (Department of Biology) Outside Member Carbohydrates are important in a large number of cellular, physiological, and pathological processes. Carbohydrates often function as the human host’s first line of defence against pathogen invasion by coating surfaces of epithelial cells and as glycanrich mucins which line the entrances to the body. Various pathogenic bacteria exploit their hosts by modifying their glycans through the production of carbohydrate-active enzymes. Two kinds of pathogenic bacteria that are notable for their production of carbohydrate-active enzymes are Streptococcus pneumoniae and Clostridium perfringens. Both S. pneumoniae and C. perfringens inhabit glycan-rich niches in the human body, the respiratory and gastrointestinal tracts, respectively. To properly colonize their human hosts both bacteria have developed an extensive repertoire of glycoside hydrolases (GHs) which are enzymes responsible for the breakdown of carbohydrates. These GHs have known or predicted specificities for human glycans, specifically those found in mucins. We chose C. perfringens and S. pneumoniae as model systems to study these enzymes due to their large complements of GHs, many of which are known virulence factors. The objectives are to probe the key features of the GHs from these two different kinds of bacteria that inhabit similar human niches and to study catalysis, modularity and overall enzyme structure. This work uses a multidisciplinary approach and provides molecular level insight into the S. pneumoniae and C. perfringens host-pathogen interaction.
Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

Specific metabolic network responses to mineral starvation are not well-defined. We examined a detailed broad-scale identification of metabolic responses of tomato leaf and root to N, P or K starvation. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and starved (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Overall, the levels of metabolites (lipids, nucleotides, peptides and secondary metabolites) presented in this paper largely showed mineral- and tissue-specific responses. Most strikingly, G3P (glycerol-3-P), GPC (glycerol-P-choline) and choline phosphate responded differently to a type of mineral; an increase in N or K starvation and a decrease in P starvation. A dramatic increase in the levels of secondary metabolites, in particular, rutin and chlorogenate in both tomato tissues during N starvation were observed. Based on these data, it is necessary to clearly elucidate an unknown event taking place in a variety of abiotic impacts, and we are now studying to expand our knowledge on metabolic- and proteomic-responses using GS-MS and LC-MS.
Identifying Developmental Language Disorder in School Age Bilinguals: Semantics, Grammar, and Narratives

ABSTRACT Children with Developmental language disorder (DLD) have particular difficulty learning language despite otherwise general normal development. When school age bilingual children struggle with language, a common question is if the difficulties they present reflect lack of ability or lack of language experience. To address the question of identification of DLD in the context of bilingualism, we explore the diagnostic accuracy of measures administered in two languages. The Bilingual English Spanish Assessment Middle Extension (BESA-ME) assesses semantics and morphosyntax and the Test of Narrative Language (TNL) assesses comprehension and production of narratives. These measures were administered to 112 second graders (19 with DLD) and 64 fourth graders (7 with DLD). We explored the classification accuracy of each of these measures alone and in combination using receiver operator characteristic (ROC) curve analysis. The ROC curve illustrates diagnostic classification of a measure at various cutpoints. We compared the extent to which measures in English, Spanish, or best language account for area under the (ROC) curve. Discriminant function analysis using the best indicator (Spanish, English, best language) from each type of measure (semantics, morphosyntax, narrative) in combination demonstrate classification accuracy above 80%. Morphosyntax in the best language was the strongest predictor of DLD for second grade children. In fourth grade, the three measures contributed more equally in predicting DLD.
Genetic Dissection of Wheat Genotypes Using Morpho-Physiological Traits for Terminal Heat Tolerance

Wheat, a self-pollinated annual temperate crop plant belongs to the true grass family Gramineae (Poaceae), is the largest cereal crop extensively grown as staple food in the world. The development of an efficient plant hybridization program is dependent upon the existence of genetic variability (Kumar et al., 2015). The magnitude of genetic variability present in the breeding materials determines the effectiveness of selection. Thus, the success in genetic improvement of any character depends on the nature of variability present in the gene pool for that character. Hence, for a crop species, it is of utmost International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 02 (2018) Journal homepage: http://www.ijcmas. com
Testosterone-related and seasonal changes in sebaceous glands in the back skin of adult male brown bears (Ursus arctos)

Adult male brown bears (Ursus arctos Linnaeus, 1758) display tree-marking behavior to chemically signal their dominance throughout the nondenning period, and this behavior peaks during the breeding season. Within the scent-marking sequence, back rub is a core marking posture. The present study investigated (i) seasonal changes in sebaceous glands in the back skin of brown bears and (ii) the relationship between those changes and testosterone levels. Back skin tissue samples and blood were collected from captive adult intact and castrated males during prebreeding, transitional, breeding, and postbreeding seasons, which were concurrent with back skin observations. In intact males, during the transitional and breeding seasons, an oily secretion from the back skin was observed along with enlarged sebaceous glands. The plasma testosterone concentrations during the transitional and breeding seasons were increased compared with the pre- and post-breeding seasons. Secretions and enlarged sebaceous glands were not.
Anthracotheriid artiodactyl Anthracokeryx and an upper Eocene age for the Youganwo Formation of southern China

Abstract The two recently collected mammalian dentary fragments from the Eocene Youganwo Formation of Guangdong Province, southern China, are referred to the anthracotheriid species Anthracokeryx naduongensis based on phylogenetic analysis and size comparison. One of these specimens (SYSU-M-1) is the first mammal fossil described from the Youganwo Formation. It was attributed previously to the perissodactyl genus Lunania. Anthracokeryx naduongensis was described originally from the lower upper Eocene Na Duong Formation in Northern Vietnam. The second record of this species supports a basal upper Eocene correlation for the Youganwo Formation, which was estimated previously as middle or late Eocene.
INTRODUCTION OF GLYOXALASE I GENE FOR SALT TOLERANCE INTO RICE (Oryza sativa L.) VARIETIES ‘PR118’ AND ‘KITAAKE’ THROUGH PARTICLE BOMBARDMENT

The present investigation dealing with introduction of glyoxalase I gene for salt tolerance into rice (Oryza sativa L.) through particle bombardment was carried out using two varieties PR118 (indica) and Kitaake (japonica). Calli were induced from both the varieties on MS medium supplemented with 2,4-D (3.0 mgL) + BAP (0.25 mgL) + proline (600 mgL) + maltose (40 gL) + phytagel (3 gL). Embryogenic calli were subcultured on shoot regeneration MS medium supplemented with BAP (4.0 mgL) + NAA (0.2 mgL) + sucrose (30 gL) + phytagel (2 gL) + agar (8 gL). An attempt was made to introduce GlyI gene into both the varieties. Mature seedderived embryogenic calli were used as explants for all the transformation experiments. Success was achieved in both the varieties. Using GlyI gene, in PR118, out of 2600 calli bombarded, 249 putative transgenic plants were regenerated on medium containing hygromycin (30 mgL). Likewise, in Kitaake, out of 615 calli bombarded, 469 putative transgenic plants were regenerated on medium containing hygromycin (30 mgL). Among the 249 regenerants of PR118, 13 plants were PCR positive and in case of Kitaake, among 615 plants 41 showed PCR positive results. Further, these plants were grown to maturity in transgenic glass house. Presence of copy number of transgene was done by Real Time PCR. In total, a set of 10 PCR positive plants (5 of PR118 and 5 of Kitaake) 10 samples were analyzed, among them sample number four has single copy gene and rest has multiple copy ranging from 2-14 copies. To conclude tissue culture base line has been established in two varieties PR118 and Kitaake. Using this baseline, a total of 54 PCR positive transgenic plants were developed. The protocol developed here is genotype independent and is suitable for japonica as well as indica varieties.