Miracle-dz/UniFAR-FTUs huggingface dataset:
Validation of the Harmless Acute Pancreatitis Score in Predicting Nonsevere Course of Acute Pancreatitis

Background: The Harmless Acute Pancreatitis Score (HAPS) is a scoring algorithm to identify patients with nonsevere acute pancreatitis. The aim of this study was to evaluate the reproducibility of HAPS outside its original study setting. Method: Baseline information of all hospitalized patients with acute pancreatitis at Karolinska University Hospital, Stockholm, Sweden, between 2004 and 2009 was collected. The parameters constituting HAPS were signs of peritonitis, hematocrit and serum creatinine levels. Since hematocrit was not available in all patients, complete sample analysis was performed by replacing hematocrit with hemoglobin (strongly correlated with hematocrit; r = 0.86). Results: In total, 531 patients with a first-time or a recurrent attack of acute pancreatitis were included. Among 353 patients with complete information on parameters constituting HAPS, 79 patients were predicted to have a nonsevere course, of whom 1 patient developed severe acute pancreatitis. The specificity of HAPS in predicting a nonsevere course of acute pancreatitis was 96.3% (95% CI: 81.0–99.9) with a corresponding positive predictive value of 98.7% (95% CI: 93.1–100). Complete sample analysis replacing hematocrit with hemoglobin level predicted a nonsevere course in 182 patients, of whom 2 patients had severe acute pancreatitis (94.3% specificity and 98.9% positive predictive value). Conclusion: HAPS is a highly specific scoring algorithm that predicts a nonsevere course of acute pancreatitis. Therefore, HAPS might be an additional tool in the clinical assessment of acute pancreatitis where early screening is important to treat the patients at an optimal level of care.
Aggressive natural killer cell leukemia: clinical features and treatment outcome.

of viable CD41+ cells be harvested at day 14 of culture. Bcl-xL mRNA was detected at high levels in all samples of RAEB CD34+ cells at day 0, and it appeared progressively down-regulated at days 7 and 14 of culture (Figure 2A). Moreover, the Bcl-xL expression pattern was similar in control cultures and in cultures containing anti-TGF-β1 neutralizing antibody (Figure 2B). The deregulated expression of Bcl-xL in MDS CD34+ cells was not dependent on impaired TGF-β1 autocrine production. In fact, we have previously demonstrated that MDS CD34+ cells induced towards megakaryocytic differentiation produce TGF-β1. 1 In conclusion, our data confirm that Bcl-xL up-regulation plays a fundamental role in normal megakaryocytopoiesis and demonstrate that it depends on the autocrine TGF-β1 produced by differentiating cells. Furthermore, this study highlights that Bcl-xL expression in RAEB CD34+ cells undergoing megakaryocytic commitment is completely altered and suggests that it can play a role in their defective differentiation capacity. Maurizio Martini,#* Myriam Luongo,#° Luciana Teofili,° Antonella Di Mario,° Giuseppe Leone,* Luigi Maria Larocca* Istituti di*Anatomia Patologica ed °Ematologia, Università Cattolica del Sacro Cuore, Rome, Italy #These authors equally contributed to this work. Funding: This study was supported in part by Grants ex-60% from Ministero dell’Università e della Ricerca, Rome, Italy. Correspondence: Maurizio Martini, M.D., Istituto di Anatomia Patologica, Università Cattolica del Sacro Cuore, largo Francesco Vito n°1, 00168, Rome, Italy. Phone: international +39.06. 30154270. Fax: international +39.06. 30155883. E-mail: llarocca@rm.unicatt. it
Electron Cryomicroscopy Reveals Different F1+F2 Protein States in Intact Parainfluenza Virions

ABSTRACT Electron cryomicrographs of intact parainfluenza virus 5 (PIV5) virions revealed two different surface structures, namely, a continuous layer and distinct individual spikes. The structure of these spikes reconstructed from intact virions was compared with known F ectodomain structures and was found to be different from the prefusion PIV5 F0 structure but, surprisingly, very similar to the human PIV3 F postfusion structure. Hence, we conclude that the individual F1+F2 spikes in intact PIV5 virions also correspond to the postfusion state. Since the observed fusion activity of PIV5 virions has to be associated with prefusion F1+F2 proteins, they have necessarily to be localized in the continuous surface structure. The data therefore strongly suggest that the prefusion state of the F1+F2 protein requires stabilization, most probably by the association with hemagglutinin-neuraminidase. The conversion of F1+F2 proteins from the prefusion toward the postfusion state while embedded in the virus membrane is topologically difficult to comprehend on the basis of established models and demands reconsideration of our current understanding.
A generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species.

Noninvasive fecal glucocorticoid analysis has tremendous potential as a means of assessing stress associated with environmental disturbance in wildlife. However, interspecific variation in excreted glucocorticoid metabolites requires careful selection of the antibody used in their quantification. We compared four antibodies for detecting the major fecal cortisol metabolites in yellow baboons following (3)H cortisol administration, ACTH challenge, and HPLC separation of fecal glucocorticoid metabolites. The most effective antibody (ICN corticosterone RIA; Cat. No. 07-120102) demonstrated relatively high cross-reactivities to the major cortisol metabolites present in feces during peak excretion, following both radiolabel infusion and ACTH challenge. This same antibody also detected increased fecal glucocorticoid metabolites after ACTH administration in the African elephant, black rhinoceros, Roosevelt elk, gerenuk, scimitar-horned oryx, Alaskan sea otter, Malayan sun bear, cheetah, clouded leopard, longtailed macaque, and northern spotted owl. Results suggest that (1) fecal glucocorticoid assays reliably detect endogenous changes in adrenal activity of a diverse array of species and (2) where comparisons were made, the ICN corticosterone antibody generally was superior to other antibodies for measuring glucocorticoid metabolites in feces.
Reliability Investigations for Bulk-FinFETs Implementing Partially-Insulating Layer

This paper presents a detailed analysis of the reliability characteristics of partially-insulated FinFETs (PI-FinFETs) where a new source/drain structure was adapted using a pad-polysilicon side contact (PSC). The PSC structure shows excellent improvements in device performances mainly due to the increment of the contact area by using lateral faces of FinFETs. The hot carrier degradation characteristics are also improved in comparison with a conventional source/drain structure having planar contact. This is due to an advantageous impact ionization position. By applying PSC structure to Pi-FinFETs, an optimized source/drain structure of PI-FinFETs can be obtained with its own advantages.
moLink: Modeling link representation of knowledge base

Knowledge bases are a significant resource for a variety of natural language processing tasks. They can be visualized as directed graphs in which nodes correspond to entities and edges represent relationships. However, knowledge bases are typically incomplete, and it is beneficial to perform knowledge base completion or link prediction, in order to describe a more complete picture of them. Embedding models for knowledge base completion aim at offering a numerical representation of entities and relations by transforming them into low dimensional vector space. In this paper, we propose a novel embedding model, called “moLink”, which is leveraging the knowledge and computing link embedding. The model is designed which is obtained high-quality distributed representation of entities and relations. Empirical experiments have proved the effectiveness of the moLink on knowledge bases.
On the separation of timescales in radio-based positioning

Radio-based positioning methods are generally based on a separation of timescales, where each position update assumes the availability of synchronous measurements. This approach is convenient in the design of positioning algorithms, but fails to account for outdated information. We describe three distinct ways how the separation of timescales can adversely impact the design and execution of positioning methods, and quantify the extent of these impacts analytically.
Procalcitonin as a prognostic marker in patients with acute myocardial infarction

Background: Procalcitonin is involved in the inflammatory response and is associated with adverse prognosis in certain conditions. Aims: To investigate the association between procalcitonin and major adverse cardiac events (MACE), left ventricular (LV) function and remodelling following acute myocardial infarction (AMI). Methods: Plasma procalcitonin was measured in 977 patients with AMI. Subjects were followed for MACE (median 671 days). A subgroup underwent echocardiography at discharge and follow-up LV function and volume assessment. Results: Procalcitonin was associated with MACE on uni- and multivariable analysis. Kaplan–Meier assessment revealed an adverse outcome in subjects with procalcitonin above the median. Procalcitonin was related to markers of LV dysfunction and remodelling. Conclusion: Procalcitonin is associated with MACE, LV dysfunction and remodelling post-AMI.
Using logic programming for adapting models to metamodel evolution

Evolution is inherent in software systems because of the rapid improvement of technologies. As metamodels are the cornerstone of model driven engineering and they evolve iteratively, their evolution affects the rest of artefacts involved in a development process, e.g., models or transformation rules. Therefore, co-evolution tools for models and other artefacts are indispensable. We present in this paper an intelligent approach to adapt models by means of state-based and operator-based techniques. The defined co-evolution process consists of four phases. Initially, changes between two metamodel versions are detected. After that, evolution scenario is reconstructed using logic programming method. Evolution scenario is first calculated from difference model and represented by a set of primitive evolution operations, after that it is transformed using an inference engine to include eventual composite evolution operations; from this scenario adaptation solutions are generated according to the evolution operator impact in model level. Finally, migration scenario is applied on an input model conforming to the old metamodel version to remain compliant with its metamodel.
Single-incision multiport laparoscopy does not cause more pain than conventional laparoscopy: a prospective evaluation in children undergoing appendectomy.

BACKGROUND The benefit of single-incision multiport laparoscopy (SIMPL) remains a matter of vivid discussion. For good reason it has been speculated that SIMPL causes more postoperative pain, because a minilaparotomy is required to place the multiport system. We prospectively evaluated postoperative pain scores and requirement of analgesic medication following conventional laparoscopic (CL) versus SIMPL appendectomy in children. METHODS The access for laparoscopic appendectomy was decided upon the surgeon's preference. Between April and October 2010, individual abdominal pain scores at 8, 16, 24, 48, and 72 hours postoperatively as well as the incidence of umbilical or shoulder pain and the total amount of peri- and postoperative analgesics, operative time, length of hospital stay, and demographics were assessed. Analgesics (paracetamol and/or metamizole, 15 mg/kg body weight) were administered regularly or on inquiry of the patient. Data are presented as means±standard deviation tested at a significance level of P<. 05. RESULTS All operations were laparoscopically completed without conversion or addition of extra ports. Thirty-nine patients (8 SIMPL appendectomy) at a mean age of 12.3±2.4 years and a mean body mass index of 19.16±3.2 kg/m(2) were included. Equal operation times were observed (SIMPL: 68.5±19.9 minutes versus CL: 66.2±19.5 minutes). There were no significant differences for the individual pain scores or the incidence of umbilical and shoulder pain between study groups. The total amount of required analgesic medication was significantly lower after SIMPL appendectomy (SIMPL: 65.73±43.8 mg/kg versus CL: 106.39±46.4 mg/kg, P=.04). CONCLUSION In summary, the present study substantiates the evidence that SIMPL appendectomy in children and adolescents is not only feasible but also beneficial for the patient without translation into increased postoperative pain. Presently, we are conducting a randomized, blinded study to validate these findings.
NADPH oxidase-derived H(2)O(2) contributes to angiotensin II-induced aldosterone synthesis in human and rat adrenal cortical cells.

BACKGROUND The Renin-Angiotensin-Aldosterone-System plays a pivotal role in hypertension. Angiotensin II (Ang II) is a major regulator of aldosterone synthesis and secretion, and it is known to facilitate reactive oxygen species (ROS) generation in many cell types. AIMS Here, we assessed the role of ROS signaling in Ang II-induced aldosterone synthesis by focusing on the regulation of aldosterone synthase (CYP11B2), a cytochrome P450 oxidase that catalyzes the final step in aldosterone biosynthetic pathway. RESULTS Ang II increased CYP11B2 activity, mRNA and protein with a concomitant elevation of 6-Carboxy- 2',7'-dichlorodihydrofluorescein diacetate fluorescence, malondialdehyde and protein carbonyl levels (indices of ROS), NADPH oxidase (Nox) activity, and H(2)O(2) levels in human and rat adrenal cortical cells. The expression of nuclear receptor related 1 protein, a transcription factor known to regulate CYP11B2 expression, was also augmented by Ang II. These Ang II-evoked effects were either abolished or attenuated by pretreatment of cells with either Ang II type I receptor (AT(1)R) antagonist, or antioxidants or Nox inhibitor or siRNA silencing of Nox1, 2 and 4, or inhibitors of phospholipase C and protein kinase C. Exogenous H(2)O(2) mimicked the facilitatory effects of Ang II on CYP11B2 activity, mRNA, and protein expression, and these changes were significantly reduced by PEG-catalase. INNOVATION ROS, particularly H(2)O(2), is identified as a key regulator of aldosterone production. CONCLUSION Our results suggest that Ang II facilitates CYP11B2 activity and the ensuing aldosterone production via activation of AT(1)R-Nox-H(2)O(2) signaling pathway.
New Anthraquinones and Anthraquinone Glycosides from Morinda lucida

Abstract From the trank heartwood of Morinda lucida four new anthraquinones, 3-hydroxy-anthraquinone-2-carbaldehyde, morindone-5-methyl ether, munjistin methyl ester, damna-canthol-ѡ-methyl ether and two glycosides, soranjidiol 6-0-β-primveroside and lucidin 3-0-β-primveroside were isolated and identified by spectroscopic and chemical methods.
Detecting Changes in Multispectral Satellite Images using Time Dependent Angle Vegetation Indices

Change detection using satellite images is of major concern to government agencies, military, and scientists. Government agencies can monitor the construction activity on a region automatically by detecting changes. They can update maps accordingly. Forests in satellite images may be monitored automatically for any change in their condition. As for military applications, strategic plans may be updated based on the observed changes on targets. After a natural disaster, even if that region can not be reached, automatically detecting changes in the region's satellite image may provide necessary information to measure the effect of the damage. To solve all these and related problems, there exists commercially available high resolution satellite images. The problem here is automatically detecting changes using these images. In this study, we introduce a method using multispectral information to detect changes on Ikonos imagery.
Bending effect on antenna with radiation performance for electronic tag

In this paper, the meander line antenna (MLA) have been designed to operate at 915MHz for RFID application. HFSS software is used for simulation designed process. The performance degradation of tag antenna due to its arc curving is investigated. The impedance, gain and maximum working distance of the designed antenna during bending was obtained.
Nuclear binding of purified retinoblastoma gene product is determined by cell cycle-regulated phosphorylation

The retinoblastoma tumor suppressor gene product (pRb) is a nuclear protein subject to cell cycle-regulated hyperphosphorylation. I constructed a recombinant vaccinia virus vector that expresses both the underphosphorylated and hyperphosphorylated forms of pRb and purified the recombinant protein by using immunoaffinity chromatography directed toward a synthetic carboxy-terminal epitope. To investigate the hypothesis that hyperphosphorylation of pRb is a means of controlling its growth-regulating activity, I tested purified pRb for the ability to be reincorporated into pRb-deficient nuclei in vitro. The underphosphorylated form of pRb efficiently reassociated with nuclei, but the hyperphosphorylated form remained soluble in this assay. Nuclear binding of pRb was enhanced by phosphatase treatment and reduced by phosphorylation of pRb effected by using a preparation of the cell cycle-regulatory kinase p34cdc2. Mutant-encoded proteins with altered E1A-binding domains failed to bind to nuclei. Pretreatment of target nuclei with nucleases and high-salt extraction did not alter the specificity of binding for underphosphorylated pRb. These observations demonstrate that hyperphosphorylation of pRb can regulate its interaction with nuclei, supporting the hypothesis that hyperphosphorylation controls the growth-regulatory activities of pRb. Further, at least one target of pRb binding appears to be an integral component of the nuclear envelope.
Development of a Rapid Assimilable Organic Carbon Method for Water

A rapid method for measurement of assimilable organic carbon (AOC) is proposed. The time needed to perform the assay is reduced by increasing the incubation temperature and increasing the inoculum density. The ATP luciferin-luciferase method quickly enumerates the test organisms without the need for plate count media or dilution bottles. There was no significant difference between AOC values determined with strain P17 for the ATP and plate count procedures. For strain NOX, the plate count procedure underestimated bacterial levels in some samples. Comparison of AOC values obtained by the Belleville laboratory (by the ATP technique) and the Stroud Water Research Center (by plate counts) showed that values were significantly correlated and not significantly different. The study concludes that the rapid AOC method can quickly determine the bacterial growth potential of water within 2 to 4 days.
Reference values of vertical jumping performances in healthy Tunisian adolescent

Abstract Background: The assessment of normal values of muscle strength can be determined for the health outcome of adolescents, especially those who are living in a developing country. Aims: The purpose of this study is to identify the relationship between anthropometric variables and vertical jumping performances. The jump height and the average of power were measured to establish reference values of vertical jumping parameters in Tunisian healthy adolescents aged 13–19 in both sexes. Subjects and methods: Five hundred and twenty-five school adolescents (242 males and 283 females) were randomly selected to participate in this study. Maximum height and average of power reached in countermovement jump and squat jump were provided by an Optojump device. Full and stepwise regression models were used to identify which anthropometric parameters significantly contributed to performance variables. Results: All anthropometric parameters increased with age. Reference values and multiple prediction equations of vertical jump parameters were set based on a large sample of healthy Tunisian adolescents. The multiple regressions showed that age, mass, sitting height, waist size, fat-free mass and leg muscle volume for boys and mass for girls were the best predictors of jumping performances. Conclusion: This study provides normative data for jumping performances in Tunisian healthy adolescents aged 13–19 in both sexes. The percentiles values are calculated to estimate the levels of adolescents with high or low jumping performances.
Improved AdaBoost Algorithm Using VQMAP for Speaker Identification

Adaptive boosting (AdaBoost) learning method can improve the performance of a base classifier by mining feature information in depth. But it is computationally expensive, and the base classifier without a suitable accuracy will cause over fitting. In this paper an improved Adaboost algorithm using maximum a posteriori vector quantization model (VQMAP) for speaker identification is presented. A suitable VQMAP classifier matched the size of speaker identification problem is constructed first. Then it is boosted to a strong classifier by AdaBoost with early stopping method. Experiments show that the performance of the boosted VQMAP classifier is better than that of VQMAP, and is slightly lower than that of maximum a posteriori adapted Gaussian mixture model (GMMMAP), but with a faster recognition speed. In the case of limited data and predictable speaker number, it will reach or exceeded GMMMAP.
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1

ABSTRACT In our previous study, virus-binding proteins (VBPs) demonstrating the ability to strongly bind poliovirus type 1 (PV1) were recovered from a bacterial culture derived from activated sludge. The isolated VBPs would be useful as viral adsorbents for water and wastewater treatments. The VBP gene of activated sludge bacteria was isolated, and the cloning system of the VBP was established. The isolation of the VBP gene from DNA libraries for activated sludge bacteria was achieved with the colony hybridization technique. The sequence of the VBP gene consisted of 807 nucleotides encoding 268 amino acids. Fifteen amino acid sequences were retrieved from 2,137,877 sequences by a homology search using the BLAST server at the National Center for Biotechnology Information. The protein encoded in the isolated genome was considered to be a newly discovered protein from activated sludge culture, because any sequences in protein databases were not perfectly matched with the sequence of the VBP. It was confirmed that Escherichia coli BL21 transformed by pRSET carrying the isolated VBP gene could extensively produce the VBP clones. Enzyme-linked immunosorbent assay (ELISA) revealed that the VBP clone exhibited the binding ability with intact particles of PV1. The equilibrium binding constant between PV1 and VBP in the ELISA well was estimated to be 2.1 × 107 (M−1), which also indicated that the VBP clones have a high affinity with the PV1 particle. The VBP cloning system developed in this study would make it possible to produce a mass volume of VBPs and to utilize them as a new material of the specific adsorbent in several technologies, including virus removal, concentration, and detection.