Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatio-Spectral Feature Representation for Motor Imagery Classification Using Convolutional Neural Networks

Convolutional neural networks (CNNs) have recently been applied to electroencephalogram (EEG)-based brain–computer interfaces (BCIs). EEG is a noninvasive neuroimaging technique, which can be used to decode user intentions. Because the feature space of EEG data is highly dimensional and signal patterns are specific to the subject, appropriate methods for feature representation are required to enhance the decoding accuracy of the CNN model. Furthermore, neural changes exhibit high variability between sessions, subjects within a single session, and trials within a single subject, resulting in major issues during the modeling stage. In addition, there are many subject-dependent factors, such as frequency ranges, time intervals, and spatial locations at which the signal occurs, which prevent the derivation of a robust model that can achieve the parameterization of these factors for a wide range of subjects. However, previous studies did not attempt to preserve the multivariate structure and dependencies of the feature space. In this study, we propose a method to generate a spatiospectral feature representation that can preserve the multivariate information of EEG data. Specifically, 3-D feature maps were constructed by combining subject-optimized and subject-independent spectral filters and by stacking the filtered data into tensors. In addition, a layer-wise decomposition model was implemented using our 3-D-CNN framework to secure reliable classification results on a single-trial basis. The average accuracies of the proposed model were 87.15% (±7.31), 75.85% (±12.80), and 70.37% (±17.09) for the BCI competition data sets IV_2a, IV_2b, and OpenBMI data, respectively. These results are better than those obtained by state-of-the-art techniques, and the decomposition model obtained the relevance scores for neurophysiologically plausible electrode channels and frequency domains, confirming the validity of the proposed approach.
Fecal Microbiota Nutrient Utilization Potential Suggests Mucins as Drivers for Initial Gut Colonization of Mother-Child-Shared Bacteria

The initial bacterial colonization of human infants is crucial for lifelong health. Understanding the factors driving this colonization will therefore be of great importance. ABSTRACT The nutritional drivers for mother-child sharing of bacteria and the corresponding longitudinal trajectory of the infant gut microbiota development are not yet completely settled. We therefore aimed to characterize the mother-child sharing and the inferred nutritional utilization potential for the gut microbiota from a large unselected cohort. We analyzed in depth gut microbiota in 100 mother-child pairs enrolled antenatally from the general population-based Preventing Atopic Dermatitis and Allergies in Children (PreventADALL) cohort. Fecal samples collected at gestational week 18 for mothers and at birth (meconium), 3, 6, and 12 months for infants were analyzed by reduced metagenome sequencing to determine metagenome size and taxonomic composition. The nutrient utilization potential was determined based on the Virtual Metabolic Human (VMH, www.vmh.life) database. The estimated median metagenome size was ∼150 million base pairs (bp) for mothers and ∼20 million bp at birth for the children. Longitudinal analyses revealed mother-child sharing (P < 0.05, chi-square test) from birth up to 6 months for 3 prevalent Bacteroides species (prevalence, >25% for all age groups). In a multivariate analysis of variance (ANOVA), the mother-child-shared Bacteroides were associated with vaginal delivery (1.7% explained variance, P = 0.0001). Both vaginal delivery and mother-child sharing were associated with host-derived mucins as nutrient sources. The age-related increase in metagenome size corresponded to an increased diversity in nutrient utilization, with dietary polysaccharides as the main age-related factor. Our results support host-derived mucins as potential selection means for mother-child sharing of initial colonizers, while the age-related increase in diversity was associated with dietary polysaccharides. IMPORTANCE The initial bacterial colonization of human infants is crucial for lifelong health. Understanding the factors driving this colonization will therefore be of great importance. Here, we used a novel high-taxonomic-resolution approach to deduce the nutrient utilization potential of the infant gut microbiota in a large longitudinal mother-child cohort. We found mucins as potential selection means for the initial colonization of mother-child-shared bacteria, while the transition to a more adult-like microbiota was associated with dietary polysaccharide utilization potential. This knowledge will be important for a future understanding of the importance of diet in shaping the gut microbiota composition and development during infancy.
Vascular biotransformation of organic nitrates is independent of cytochrome P450 monooxygenases

Organic nitrates such as nitroglycerin (NTG) or pentaerythritol tetranitrate (PETN) have been used for over a century in the treatment of angina or ischaemic heart disease. These compounds are prodrugs which release their nitrovasodilators upon enzymic bioactivation by aldehyde dehydrogenase (ALDH2) or cytochromes P450 (CYP). Whereas ALDH2 is known to directly activate organic nitrates in vessels, the contribution of vascular CYPs is unknown and was studied here.
Computerized Assessment of Occlusion and Muscle Activity during Use of a Multilayer Clear Retainer: A Preliminary Study

The aim of this preliminary study was to evaluate the short-term changes of occlusal contacts and muscle activity after orthodontic treatment during the use of a multi-layer clear retainer. Evaluation was done with the T-scan and BioEMG systems. A total of 18 subjects were included, who were evaluated at three time intervals—T0 at debonding, T1 at one month after retainer delivery, and T2 at four months after retainer delivery. The T-scan and electromyography (EMG) data were recorded simultaneously. The T-scan system recorded the occlusion time, disclusion time and force distribution. The EMG waves were quantified by calculating the asymmetry index and activity index. The time variables changed but not significantly. Occlusal force decreased in the anterior dentition and increased in the posterior dentition during T0–T2. There was no clear evidence of a relationship between unbalanced occlusal forces and muscle activity. In most subjects, the temporalis anterior muscle was more dominant than the masseter muscle. From this preliminary computerized study, there were no significant changes in the state of the occlusion or muscle activity during the short-term retention period.
The ATR-WEE1 kinase module inhibits the MAC complex to regulate replication stress response

Abstract DNA damage response is a fundamental mechanism to maintain genome stability. The ATR-WEE1 kinase module plays a central role in response to replication stress. Although the ATR-WEE1 pathway has been well studied in yeasts and animals, how ATR-WEE1 functions in plants remains unclear. Through a genetic screen for suppressors of the Arabidopsis atr mutant, we found that loss of function of PRL1, a core subunit of the evolutionarily conserved MAC complex involved in alternative splicing, suppresses the hypersensitivity of atr and wee1 to replication stress. Biochemical studies revealed that WEE1 directly interacts with and phosphorylates PRL1 at Serine 145, which promotes PRL1 ubiquitination and subsequent degradation. In line with the genetic and biochemical data, replication stress induces intron retention of cell cycle genes including CYCD1;1 and CYCD3;1, which is abolished in wee1 but restored in wee1 prl1. Remarkably, co-expressing the coding sequences of CYCD1;1 and CYCD3;1 partially restores the root length and HU response in wee1 prl1. These data suggested that the ATR-WEE1 module inhibits the MAC complex to regulate replication stress responses. Our study discovered PRL1 or the MAC complex as a key downstream regulator of the ATR-WEE1 module and revealed a novel cell cycle control mechanism.
Cardiogenic shock teams and centres: a contemporary review of multidisciplinary care for cardiogenic shock

Cardiogenic shock (CS) portends high morbidity and mortality in the contemporary era. Despite advances in temporary mechanical circulatory supports (MCS), their routine use in CS to improve outcomes has not been established. Delays in diagnosis and timely delivery of care, disparities in accessing adjunct therapies such revascularization or MCS, and lack of a systematic approach to care of CS contribute to the poor outcomes observed in CS patients. There is growing interest for developing a standardized multidisciplinary team‐based approach in the management of CS. Recent prospective studies have shown feasibility of CS teams in improving survival across a spectrum of CS presentations. Herein, we will review the rationale for CS teams focusing on evidence supporting its use in streamlining care, optimizing revascularization strategies, and patient identification and MCS selection. The proposed structure and flow of CS teams will be outlined. An in‐depth analysis of four recent studies demonstrating improved outcomes with CS teams is presented. Finally, we will explore potential implementation hurdles and future directions in refining and widespread implementation of dedicated cross‐specialty CS teams.
Binary Amplitude Reflection Gratings for X-ray Shearing and Hartmann Wavefront Sensors

New, high-coherent-flux X-ray beamlines at synchrotron and free-electron laser light sources rely on wavefront sensors to achieve and maintain optimal alignment under dynamic operating conditions. This includes feedback to adaptive X-ray optics. We describe the design and modeling of a new class of binary-amplitude reflective gratings for shearing interferometry and Hartmann wavefront sensing. Compact arrays of deeply etched gratings illuminated at glancing incidence can withstand higher power densities than transmission membranes and can be designed to operate across a broad range of photon energies with a fixed grating-to-detector distance. Coherent wave-propagation is used to study the energy bandwidth of individual elements in an array and to set the design parameters. We observe that shearing operates well over a ±10% bandwidth, while Hartmann can be extended to ±30% or more, in our configuration. We apply this methodology to the design of a wavefront sensor for a soft X-ray beamline operating from 230 eV to 1400 eV and model shearing and Hartmann tests in the presence of varying wavefront aberration types and magnitudes.
A Systematic Review of the Legal Considerations Surrounding Medicines Management

Background and Objectives: There is a paucity of integrated knowledge regarding legal considerations required to ensure patient safety through safe medicines management. This study explores the legal considerations surrounding medicines management, providing a synthesis of existing knowledge. An integrative systematic review of the current international knowledge was performed. Materials and Methods: The search encompassed the online databases of PubMed (including Medline), Scopus, CINAHL, and Web of Science using MeSH terms and relevant keywords relating to the legal considerations of medicines management in healthcare settings. Results: The search process led to the identification of 6051 studies published between 2010 and 2020, of which six articles were found to be appropriate for data analysis and synthesis based on inclusion criteria. Research methods were varied and included qualitative interviews, mixed-methods designs, retrospective case reports and cross-sectional interrupted time-series analysis. Their foci were on the delegation of medicines management, pharmacovigilance and reporting of adverse drug reactions (ADRs) before and after legislation by nurses, physicians and pharmacists, medico-legal litigation, use of forced medication and the prescription monitoring program. Given the heterogenicity of the studies in terms of aims and research methods, a meta-analysis could not be performed and, therefore, our review findings are presented narratively under the categories of ‘healthcare providers’ education and monitoring tasks’, ‘individual and shared responsibility’, and ‘patients’ rights’. Conclusion: This review identifies legal aspects surrounding medicines management, including supervision and monitoring of the effects of medicines; healthcare providers’ knowledge and attitudes; support and standardised tools for monitoring and reporting medicines’ adverse side effects/ADRs; electronic health record systems; individual and shared perceptions of responsibility; recognition of nurses’ roles; detection of sentinel medication errors; covert or non-voluntary administration of medication, and patient participation.
Is There an Association between Executive Function and Receptive Vocabulary in Bilingual Children? A Longitudinal Examination

Dual language management has been proposed as the reason for bilingual children’s sometimes enhanced executive functioning (EF). We sought to identify the directionality of the relation between language proficiency and EF, using measures of receptive vocabulary, inhibitory control, and cognitive flexibility. Data were collected twice, a year apart, on 35- to 66.8-month-old bilingual (n = 41, M = 49.19 months) and monolingual preschool children (n = 37, M = 47.82 months). The longitudinal results revealed that while the monolingual children’s vocabulary at Time 1 predicted EF at Time 2, EF at Time 1 did not predict vocabulary at Time 2. In contrast, for bilingual children the relation was not present at all. The results were similar after the one-time analyses. The absence of relations between EF and language in bilinguals, while present in monolinguals, challenges the current conceptualization of the EF advantage in bilinguals, and emphasizes the need for more research on the development of bilingual children.
Contamination Sources and Transmission Routes for Campylobacter on (Mixed) Broiler Farms in Belgium, and Comparison of the Gut Microbiota of Flocks Colonized and Uncolonized with Campylobacter

Biosecurity seems to be the most promising tool for Campylobacter control on poultry farms. A longitudinal molecular epidemiological study was performed during two production cycles, in which the broilers, the poultry house, and the environment of 10 (mixed) broiler farms were monitored weekly. Cecal droppings from the second production cycle were also used for 16S metabarcoding to study the differences in the microbiota of colonized and uncolonized flocks. Results showed that 3 out of 10 farms were positive for Campylobacter in the first production cycle, and 4 out of 10 were positive in the second. Broilers became colonized at the earliest when they were four weeks old. The majority of the flocks (57%) became colonized after partial depopulation. Before colonization of the flocks, Campylobacter was rarely detected in the environment, but it was frequently isolated from cattle and swine. Although these animals appeared to be consistent carriers of Campylobacter, molecular typing revealed that they were not the source of flock colonization. In accordance with previous reports, this study suggests that partial depopulation appears to be an important risk factor for Campylobacter introduction into the broiler house. Metabarcoding indicated that two Campylobacter-free flocks carried high relative abundances of Megamonas in their ceca, suggesting potential competition with Campylobacter.
The Application of NMR Spectroscopy and Chemometrics in Authentication of Spices

Spices and herbs are among the most commonly adulterated food types. This is because spices are widely used to process food. Spices not only enhance the flavor and taste of food, but they are also sources of numerous bioactive compounds that are significantly beneficial for health. The healing effects of spices are connected with their antimicrobial, anti-inflammatory and carminative properties. However, regular consumption of adulterated spices may cause fatal damage to our system because adulterants in most cases are unhealthy. For that reason, the appropriate analytical methods are necessary for quality assurance and to ensure the authenticity of spices. Spectroscopic methods are gaining interest as they are fast, require little or no sample preparation, and provide rich structural information. This review provides an overview of the application of NMR spectroscopy combined with chemometric analysis to determine the quality and adulteration of spices.
Theoretical Evaluation of Novel Thermolysin Inhibitors from Bacillus thermoproteolyticus. Possible Antibacterial Agents

The search for new antibacterial agents that could decrease bacterial resistance is a subject in continuous development. Gram-negative and Gram-positive bacteria possess a group of metalloproteins belonging to the MEROPS peptidase (M4) family, which is the main virulence factor of these bacteria. In this work, we used the previous results of a computational biochemistry protocol of a series of ligands designed in silico using thermolysin as a model for the search of antihypertensive agents. Here, thermolysin from Bacillus thermoproteolyticus, a metalloprotein of the M4 family, was used to determine the most promising candidate as an antibacterial agent. Our results from docking, molecular dynamics simulation, molecular mechanics Poisson–Boltzmann (MM-PBSA) method, ligand efficiency, and ADME-Tox properties (Absorption, Distribution, Metabolism, Excretion, and Toxicity) indicate that the designed ligands were adequately oriented in the thermolysin active site. The Lig783, Lig2177, and Lig3444 compounds showed the best dynamic behavior; however, from the ADME-Tox calculated properties, Lig783 was selected as the unique antibacterial agent candidate amongst the designed ligands.
Three‐Body Association Reactions of NO+ with O2, N2, and CO2

The association of NO+ with CO2, NO++CO2+He→NO+·CO2+He, is found to occur with a rate constant 1 × 10−29 cm6 sec−1 at 197°K, decreasing at higher temperatures. When N2 or Ar are used as the third body the rate constant is about 3 times larger. The association of NO+ with O2 and N2 was found to be very much less efficient. It was found that NO+·CO2 reacts rapidly with NO to produce NO+·NO, with H2O to produce NO+·H2O, and with NH3 to produce NO+·NH3. It was found that N4+ charge transfers rapidly with O2, and N3+ reacts with O2 to produce NO+, O2+, and NO2+.
Diagnostic Accuracy of Amyloid versus 18F‐Fluorodeoxyglucose Positron Emission Tomography in Autopsy‐Confirmed Dementia

The purpose of this study was to compare the diagnostic accuracy of antemortem 11C‐Pittsburgh compound B (PIB) and 18F‐fluorodeoxyglucose (FDG) positron emission tomography (PET) versus autopsy diagnosis in a heterogenous sample of patients.
Modeling Aqueous Silica Chemistry in Alkali Media

A method for modeling the reactions of siliceous species is presented, which allows chemically accurate deprotonation and dimerization energetics to be calculated, at conditions reflecting those of high pH hydrothermal zeolite synthesis. The free energies of condensation reactions leading to silicate species up to the linear tetramer are considered at room temperature and at 450 K.
Comment: Advances in Studying the Vocal Expression of Emotion: Current Contributions and Further Options

I consider the five contributions in this special section as evidence that the research area dealing with the vocal expression of emotion is advancing rapidly, both in terms of the number of pertinent empirical studies and with respect to an ever increasing sophistication of methodology. I provide some suggestions on promising areas for future interdisciplinary research, including work on emotion expression in singing and the potential of vocal symptoms of emotional disorder. As to the popular discussion of the respective role of universality versus language/culture differences, I suggest to move on from exclusively studying the accuracy of recognition in judgment studies to a more differentiated approach adding production aspects, taking into account the multiple vocal and acoustic features that interact to communicate emotion.
The applicability of disintegration tests for cohesive organic soils

AbstractThe use of ripened fine-grained organic dredged materials as construction materials, e.g. as top soil on slopes such as landfills or dikes, is an important contribution to environmental engineering science. The materials are legally considered a waste and need to be beneficially re-used. Therefore, not only standard geotechnical parameters have to be determined but also their erosion resistance which is a particularly critical environmental parameter. There is a variety of different tests to determine the flow dependent erosion resistance of soils, such as the erosion function apparatus (Briaud et al. 2001). In this study, however, the focus lays on the aggregate stability as an indicator for the erosion resistance under static loading, which can be determined using wet sieving and disintegration tests. The disintegration tests after Weismann (2003) and Endell (RPW 2006) have a similar setup; however, the specific boundary conditions for the tests as well as the evaluation procedures are different.
The Synthesis of Methylene Blue Photosensitiser Conjugated with Gold Nanoparticles

Nowadays, nanocarriers possess growing interest for medical usage such as photodynamic therapy. Conjunction of these nanoparticles with photosensitising drugs leads to more cellular uptake, higher photocytotoxicty, higher biocompatibility and more selectivity. Previous papers conclude that the 50 nm size nanoparticles give the highest cellular uptake for the purpose of photodynamic therapy. In this paper the gold nanoparticles were synthesized based on TurkevichFrens method. Moreover, methylene blue as a relevant photosensitiser was conjugated to achieved AuNPs. The spectrometric results of UV-visible and Fourier Transform Infrared spectroscopy (FTIR) as well as Atomic Force Microscopy (AFM) emphasis on the presence of MB sensitizers connected to resulting 50 nm gold nanoparticles.
A Large 13CO deficit in the lower Antarctic stratosphere due to “ozone hole” chemistry: Part II, Modeling

Recently, isotopically extremely light CO (δ13C = −32 to −43‰) was found in whole air samples from the high southern-latitude lower stratosphere (Part I). To investigate the cause of these unprecedented observations, we simulate in a photochemical model the typical history of air masses at polar latitudes in the lowermost stratosphere over austral winter and spring. The model results show that the observations may be explained by the very efficient isotope fractionation in the reaction R1: CH4 + Cl → HCl + CH3. Extremely high levels of Cl atom concentrations prevail in the polar lower stratosphere in austral spring for a period of about 10 days after most ozone is lost. Therefore, R1 becomes very efficient, leading to a very high rate of buildup of HCl and an unusually high isotopic CO fractionation.