Miracle-dz/UniFAR-FTUs huggingface dataset:
Riparian Cottonwood Trees and Adjacent River Sediments Have Different Microbial Communities and Produce Methane With Contrasting Carbon Isotope Compositions

Rivers and their adjacent riparian forests are intimately linked by the exchange of water, nutrients, and organic matter. Both riparian cottonwood trees and adjacent river sediments host microbial communities including archeal methanogens, supporting methane production and emission to the atmosphere. Here we combine microbial community and in vitro stable isotope analyses to characterize the drivers of methane cycling in distinct anoxic habitats (river sediments vs. riparian cottonwood stems) associated with the Oldman River, southern Alberta (Canada). We demonstrate that, differences in the chemical characteristics of organic matter support divergent microbial communities that generate methane from distinct metabolic pathways. Organic matter in river sediments had C/N ratios approximately 50‐fold lower than in tree stems and had more diverse dissolved organic components. Contrasting substrate availability between river sediment and tree stems was likely the primary mechanism for the greater microbial diversity in river sediments than in tree stems, the significantly different bacterial communities, and the trend toward differing abundance of methanogen orders. The methane carbon isotope composition (δ13C values) differed for the tree stem (−103.6‰ to −70.6‰) and river sediment (−55.1‰ to −48.4‰) environments, suggesting that methane was primarily produced via CO2‐reduction in tree stems by Methanobacteriales, while river sediments produced more methane through acetate fermentation primarily by Methanosarcinales. This study demonstrates the importance of organic matter quality and microbial community composition in driving metabolic processes contributing to methane production and emission in rivers and adjacent riparian forests.
DIFFERENTIALLY PRIVATE TRAFFIC PADDING FOR WEB APPLICATIONS

The wide adoption of Web applications in various sectors of our society, such as government, finance, education, health care, media, etc., has implicitly introduced new security challenges. Among such challenges are side channel attacks that may disclose private user inputs from encrypted raffic. Such attacks might have a serious impact upon user privacy in such applications. In this thesis, we propose a new concept and algorithms that can preserve user privacy in Web applications. In order to achieve this, we define a new privacy model based on a well known concept, namely, differential privacy. The intent is to make padded traffic differentially private such that adversaries cannot infer private user inputs even when they possess prior knowlege about such inputs. At the same time, we intent to achieve a balance bewteen privacy and the incurred communication overhead. In order to demonstrate the usefulness of our model, we implement the proposed algorithms and conduct experiments based on data collected from well known Web applications.
Diet composition of abundant fish species in the shallow waters of the Todos os Santos Bay, Bahia, Brazil

Coastal habitats have great ecological importance with estuarine environments providing feeding sites for many fish species, especially during juvenile life stages. This study investigates the diet composition and trophic guild organization of the common and abundant ichthyofauna in shallow areas of the Todos os Santos Bay (TSB), Bahia, Brazil, and their relationships with environmental parameters. Six fish sampling campaigns were carried out in the shallow zones (infralittoral) of the Paraguaçu River estuary which encompasses the inner and outer (marine exposure) reaches of the TSB. The stomach contents of 1231 individuals belonging to common and abundant species were examined. A total of 32 food items were identified and fish were into four trophic guilds: detritivores, zoobenthivores I, zoobenthivores II, and zooplanktivores. The relationship between the guilds and environmental parameters showed that zoobenthivores II and zooplanktivores guilds had a positive relationship with salinity and pH, explaining 86% of data variability, though only the salinity variable was significant. This study provides basic information about the diet composition of common and abundant species in the TSB and their organization into trophic guilds. In addition, demonstrates that shallow waters fish across all guilds have broadly similar diets, comprising food items that are usually associated with bottom waters.
Topology Optimization of Total Femur Structure: Application of Parameterized Level Set Method Under Geometric Constraints

Optimization of the femur prosthesis is a key issue in femur replacement surgeries that provide a viable option for limb salvage rather than amputation. To overcome the drawback of the conventional techniques that do not support topology optimization of the prosthesis design, a parameterized level set method (LSM) topology optimization with arbitrary geometric constraints is presented. A predefined narrow band along the complex profile of the original femur is preserved by applying the contour method to construct the level set function, while the topology optimization is carried out inside the cavity. The Boolean R-function is adopted to combine the free boundary and geometric constraint level set functions to describe the composite level set function of the design domain. Based on the minimum compliance goal, three different designs of 2D femur prostheses subject to the target cavity fill ratios 34%, 54%, and 74%, respectively, are illustrated.
The mediating role of competitive orientation in determining self-construal in unethical decision-making

PurposeThe ethics literature has focused on the influence of self-construal dimensions on unethical decision-making. However, the literature is unclear about why these self-construal dimensions (Independent-self, Relational-self, Collective-self) impact differently on unethical decision-making. Based on the theory of cooperation and competition, this study empirically examines the mediating role of competitive orientation and addresses the theoretically unexplained question of why self-construal dimensions influence differently on unethical decision-making.Design/methodology/approachBased on the deductive approach, a quantitative research study was conducted on the Sri Lankan banking industry because there have been many instances of unethical behavior reported in this sector lately. Data were collected from 305 bank branch managers using a structured survey questionnaire.FindingsThe findings revealed that competitive orientation mediates the self-construal dimensions and explained that competitive orientation is one reason why independent-self, relational-self and collective-self influence differently on unethical decision-making.Originality/valueThis paper addresses the unanswered question of why self-construal dimensions relate to unethical decision-making differently.
International Journal of Systems Biology and Biomedical Technologies

1 Implementation of Recursive Brute Force for Solving Motif Finding Problem on Multi-Core Marwa A. Radad, Department of Computer Science and Engineering, Faculty of Electronic Engineering, Menoufia University, Menouf, Egypt Nawal A. El-Fishawy, Department of Computer Science and Engineering, Faculty of Electronic Engineering, Menoufia University, Menouf, Egypt Hossam M. Faheem, Department of Computer Systems, Faculty of Computer and Information Sciences, Ain Shams University, Cairo, Egypt
β-D-galactopyranoside Seco-phytoporphyrin from Atropa Belladonna and Solanum Tuberosum Yellow Leaves Determined by Nuclear Magnetic Resonance

The study described the isolation of β-D-galactopyranoside seco-phytoporphyrin from Atropa belladonna and Solanum tuberosum yellow leaves. Column chromatography was applied for its isolation. The UV-Vis, mass spectrometry and NMR spectroscopy were used for the structure confirmation. The results demonstrate that the two plants that the same chlorophyll catabolite structure.
Feasibility of bare fiber Bragg grating sensing technology in the measurement of inner wall axial stress along open‐ended pipe pile in sand

Larger‐diameter open‐ended pipe piles are widely used as foundation for offshore structures. The lack of high‐quality inner wall axial stress measurement results in difficulties in loading analysis of open‐ended pipe piles during penetration and service period due to the effect of soil plugging. In this paper, two groups of model tests to assess the feasibility of bare fiber Bragg grating (FBG) sensing technology in monitoring the inner wall axial stress profile of open‐ended aluminous pipe piles during penetration and horizontal loading are presented. One open‐ended aluminous pipe pile was instrumented with bare FBG sensors and then penetrated into the sand bed with the rate of 20 and 30 mm/min, respectively. Two horizontal static loading tests were also conducted after 24‐h penetration. The installation process of bare FBG sensors along open‐ended aluminous pipe pile is introduced. The distribution profiles of axial stress and unit shaft resistance on inner wall and outer wall along model pile is discussed in detail. Model test results indicated that the bare FBG sensing technology was feasible to measure the axial stress of inner wall along open‐ended pipe piles during penetration and horizontal loading in sand. During horizontal loading, the average values of qso/qsi are 0.717 and 0.727, respectively, for model pile with penetration rate of 20 and 30 mm/min. The axial stress difference of inner wall and outer wall along model pile in penetration and horizontal loading is the result of a combination of the inside and outside soil friction as well as of the pipe in‐wall shear stiffness.
Calcium-/Calmodulin-Dependent Protein Kinase II (CaMKII) Inhibition Induces Learning and Memory Impairment and Apoptosis

Objectives Inhibition of calcium-/calmodulin- (CaM-) dependent kinase II (CaMKII) is correlated with epilepsy. However, the specific mechanism that underlies learning and memory impairment and neuronal death by CaMKII inhibition remains unclear. Materials and Methods In this study, KN93, a CaMKII inhibitor, was used to investigate the role of CaMKII during epileptogenesis. We first identified differentially expressed genes (DEGs) in primary cultured hippocampal neurons with or without KN93 treatment using RNA-sequencing. Then, the impairment of learning and memory by KN93-induced CaMKII inhibition was assessed using the Morris water maze test. In addition, Western blotting, immunohistochemistry, and TUNEL staining were performed to determine neuronal death, apoptosis, and the relative signaling pathway. Results KN93-induced CaMKII inhibition decreased cAMP response element-binding (CREB) protein activity and impaired learning and memory in Wistar and tremor (TRM) rats, an animal model of genetic epilepsy. CaMKII inhibition also induced neuronal death and reactive astrocyte activation in both the Wistar and TRM hippocampi, deregulating mitogen-activated protein kinases. Meanwhile, neuronal death and neuron apoptosis were observed in PC12 and primary cultured hippocampal neurons after exposure to KN93, which was reversed by SP600125, an inhibitor of c-Jun N-terminal kinase (JNK). Conclusions CaMKII inhibition caused learning and memory impairment and apoptosis, which might be related to dysregulated JNK signaling.
Engineered ACE2 counteracts vaccine-evading SARS-CoV-2 Omicron variant

The novel SARS-CoV-2 variant, Omicron (B.1.1.529) contains an unusually high number of mutations (>30) in the spike protein, raising concerns of escape from vaccines, convalescent sera and therapeutic drugs. Here we analyze the alteration of neutralizing titer with Omicron pseudovirus. Sera obtained 3 months after double BNT162b2 vaccination exhibit approximately 18-fold lower neutralization titers against Omicron than parental virus. Convalescent sera from Alpha and Delta patients allow similar levels of breakthrough by Omicron. Domain-wise analysis using chimeric spike revealed that this efficient evasion was primarily achieved by mutations clustered in the receptor-binding domain, but that multiple mutations in the N-terminal domain contributed as well. Omicron escapes a therapeutic cocktail of imdevimab and casirivimab, whereas sotrovimab, which targets a conserved region to avoid viral mutation, remains effective. The ACE2 decoy is another virus-neutralizing drug modality that is free, at least in theory, from complete escape. Deep mutational analysis demonstrated that, indeed, engineered ACE2 prevented escape for each single-residue mutation in the receptor-binding domain, similar to immunized sera. Engineered ACE2 neutralized Omicron comparable to Wuhan and also showed a therapeutic effect against Omicron infection in hamsters and human ACE2 transgenic mice. Like previous SARS-CoV-2 variants, some sarbecoviruses showed high sensitivity against engineered ACE2, confirming the therapeutic value against diverse variants, including those that are yet to emerge. One Sentence Summary Omicron, carrying ∼30 mutations in the spike, exhibits effective immune evasion but remains highly susceptible to blockade by engineered ACE2.
A Nonsense Variant in CCDC65 Gene Causes Respiratory Failure Associated with Increased Lamb Mortality in French Lacaune Dairy Sheep

We recently demonstrated that the Lacaune deficient homozygous haplotype 6 (LDHH6) potentially hosts a recessive perinatal lethal mutation in Lacaune dairy sheep mapped on OAR3. In the present study, we have analyzed the whole-genome sequences of two Lacaune ram heterozygous carriers of LDHH6. After variant calling and filtering against the variants of 86 non-carrier rams, we have identified a single nucleotide variant (SNV) in the two LDHH6 carriers whose variant allele induced a premature stop codon (p.Glu111*) in the Coiled-Coil Domain Containing 65 (CCDC65) gene. CCDC65 is involved in the assembly of the nexin-dynein regulatory complex for the formation of microtubules in ciliated cells. In order to identify the phenotype in homozygous sheep, we generated at-risk matings (n = 17) between rams and ewes heterozygous for the candidate variant in CCDC65. A total of 16 lambs were born alive with five genotyped as homozygous carriers. The homozygous lambs suffered from respiratory problems, and four of them died within the first month of life. At necropsy, we observed a broad hepatization of lung lobes possibly induced by infectious pneumonia. The management of this lethal recessive allele (frequency of 0.06) through reasoned mating in the Lacaune sheep selection schemes could reduce lamb mortality by 2%.
Determining Factors Of Long-Term Orientation Of Firm-Supplier Relationships: An Empirical Analysis In The Context Of The Spanish Agro-Food Sector

In an increasingly competitive and dynamic economic environment, the establishment, development and maintenance of enduring relationships between suppliers and customers is critical for both agents, and represents an important source of competitive advantages for those companies able to manage them adequately. Taking as reference the perspective of the customer, we consider that it is important for them to always work with a group of suppliers that have demonstrated an ability to adapt to their specific needs and thereby to reduce the level of uncertainty associated with their supply function. The current work, which takes the Spanish agro-food sector as framework of reference, analyzes the importance of certain factors in the long-term orientation of these relationships.
CXCL12 in Pancreatic Cancer: Its Function and Potential as a Therapeutic Drug Target

Simple Summary Pancreatic cancer is a challenging disease to treat effectively. Fibroblasts associated with pancreatic cancer contribute to disease progression by secreting factors that enhance tumor cell survival and help tumor cells avoid detection by the immune system. This overview focuses on a chemokine, CXCL12, produced by cancer-associated fibroblasts and how CXCL12 signaling enhances pancreatic cancer progression by contributing to various hallmarks of cancer including, but not limited to, tumor growth and evasion of immune response. These pro-oncogenic functions of CXCL12 make it an attractive target in pancreatic cancer. We discuss the different approaches in development to therapeutically target CXCL12 and finally propose a novel approach, the use of the farnesyl transferase inhibitor tipifarnib to inhibit CXCL12 expression in pancreatic fibroblasts. Abstract Pancreatic ductal adenocarcinoma (PDAC) is a disease with limited therapeutic options and dismal long-term survival. The unique tumor environment of PDAC, consisting of desmoplastic stroma, immune suppressive cells, and activated fibroblasts, contributes to its resistance to therapy. Activated fibroblasts (cancer-associated fibroblasts and pancreatic stellate cells) secrete chemokines and growth factors that support PDAC growth, spread, chemoresistance, and immune evasion. In this review, we focus on one such chemokine, CXCL12, secreted by the cancer-associated fibroblasts and discuss its contribution to several of the classical hallmarks of PDAC and other tumors. We review the various therapeutic approaches in development to target CXCL12 signaling in PDAC. Finally, we propose an unconventional use of tipifarnib, a farnesyl transferase inhibitor, to inhibit CXCL12 production in PDAC.
A Calculation Method for the Deformation Behavior of Warp-Knitted Fabric

Abstract In this paper, a new method to simulate the structure and loop deformation behavior of double-bar reflex-lapping warp-knitted fabrics based on the structural characteristics is proposed. A simplified mass-spring model was built in which loops knitted by filaments were considered as particles with the uniform mass distribution connected by structure springs for overlaps and shear springs for underlaps. Deformation forces and direction on particles were analyzed to describe the displacement and deformation behavior of particles. A loop model with eight control points was established, and the relationship between control points and particles was studied combining the quadratic Bezier curves. The deformation simulation was implemented by a simulator program with C# and JavaScript via web technology on Visual Studio 2015. The stereoscopic sense of filaments was realized by changing the direction and intensity of the light. The results show that the fabric deformation and the loop shape can be accurately achieve using the simplified mass-spring model compared with the real sample.
The Effect of Knowledge Management, Skill and Attitude on Employee Performance at Telkom Education Foundation

This research aims to investigate the effect of knowledge management, skills, and attitudes on employee performance at Telkom Bandung Education Foundation. This study using quantitative methods with multiple linear descriptive analysis. Regression analysis, and non-probability sampling with saturated sampling type. The number of respondents in this study was 77 respondents. Research conducted by researchers shows that there is a significant effect of knowledge management to skills, attitudes and employee performance at Telkom Bandung Education Foundation.
Investigation of Circulating Serum MicroRNA-328-3p and MicroRNA-3135a Expression as Promising Novel Biomarkers for Autism Spectrum Disorder

Abstract Circulating microRNAs (miRNAs) are emerging as promising diagnostic biomarkers for autism spectrum disorder (ASD), but their usefulness for detecting ASD remains unclear. Nowadays, development of promising biomarkers for ASD remains a challenge. Recently, dysregulation of the miRNAs expression in postmortem brain tissue, serum and peripheral blood, have been associated with ASD. Circulating miRNAs are known to be secreted by a number of different cells and can interpose delivery of information into receiver cells, thus affecting their functions. Based on this fact, it is supposed that serum miRNAs could be a novel class of biomarkers for prognosis or diagnosis of pathological disorders including ASD. In the current research, we investigated whether the expression patterns of circulating miRNAs showed dysregulation in subjects diagnosed with ASD. Expression levels of serum miR-328-3p and miR-3135a were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) method of subjects diagnosed with ASD in comparison with healthy control subjects. Our data showed that miR-328-3p and miR-3135a were substantially down-regulated in ASD patients than in those of healthy control subjects. Moreover, target gene analysis of altered serum miRNAs displayed that these molecules targeted 162 genes denoted as unique validated targets in the miRWalk database, 71 of which appear to participate in biological pathways involved in synaptic pathways and neurodegenerative condition such as Alzheimer, Huntington and Parkinson diseases. Finally, the results strongly suggested that dys-regulated serum miRNAs might be involved in molecular pathways associated with ASD and miR-328-3p and miR-3135a have the potential to be promising novel biomarkers for ASD.
Perceived Stigma Regarding Mental Illnesses among Rural Adults in Vellore, Tamil Nadu, South India

Background: Stigma is an important factor that determines whether individuals seek treatment for mental illnesses. Studies assessing public perceptions regarding mental illnesses are scarce in India. This study documents the stigma perceived by a rural population toward patients with mental illness and their families. Materials and Methods: A cross-sectional pilot study was done in five villages, selected by simple random sampling, from a rural block in Vellore, Tamil Nadu. Households in each village were selected by systematic random sampling. From the selected households, 150 subjects aged 18–65 years, without known mental disorders, were chosen by convenience sampling, based on availability. Stigma was assessed using the Devaluation of Consumers Scale (DCS) and Devaluation of Consumer's Families Scale (DCFS). Results: The proportion with high perceptions of stigma associated with mentally ill persons was 63.8%, among the 150 interviewed rural respondents (women: 112, median age: 37 years). The proportion which perceived that there was public stigma toward families of those with mental illnesses was 43.4%. Older respondents (>37 years) had higher perceptions of stigma (odds ratio: 2.07; 95% confidence interval: 1.02–4.20) than others. Conclusion: The high perception of stigma associated with persons who are mentally ill as well as their families needs to be kept in mind while planning interventions to decrease the treatment gap for psychiatric morbidity, especially in rural areas.