Miracle-dz/UniFAR-FTUs huggingface dataset:
Zero Gravity Dynamics of Two-Phase Flow In a Circular Cylinder

The current study investigates the dynamics of a two phase flow in a circular cylinder under zero gravity conditions. Zero gravity fluid mechanics is applicable in both spaceflight and terrestrial applications. Plug formation obstruct lung passages and is the cause of asthma and pulmonary disease . In spaceflight applications, the helium bubbles occlude fuel lines of hydrazine arc-jet thrusters on satellites which cause operational problems. The process of plug formation is not fully understood. Previous studies have been limited to static solutions in cylinders. The goal of the current study is to investigate the dynamics of two phase flow under zero gravity conditions and low bond numbers. The specific objectives of this study are to model a two-phase flow into a circular cylinder via a side tube in zero gravity and confirm the existence of droplet, annulus, and plug topologies through experiment and simulation. Interim stages between topologies and steps of transition are analyzed in terms of flow quantities such as flow rate and kinetic energy. The effect of varying inlet pressure on topology formation is also determined and investigated. An experiment was conducted to determine the dynamics of silicone oil-air interface in a cylinder under low bond number conditions using a high speed camera to capture the fluid. Numerical simulations were carried out for both static and dynamic conditions. Results confirmed the existence of droplet, annulus and plug for the dynamic system. A characteristic time where plug is formed is identified and has a value of approximately 13. An instability regime between the annulus and plug was identified which gets narrower for higher pressure differences. Stable plug solutions are delayed for lower pressure differences. Stable plug solutions in dynamic systems were found to be higher than those in static systems under the same conditions.
Rape Crisis Victim Advocacy: A Systematic Review

While rape crisis center (RCC) advocacy is generally regarded as valuable, there are no prior systematic reviews of the advocacy literature. This review examined RCC advocacy service provision, perceptions and impact of advocacy, and challenges and facilitators to effective service provision. Databases related to health and social sciences were searched including Academic Search Complete, PsychINFO, PubMed, CINAHL, ProQuest, Science Direct, OAlster, WorldCat, and MEDLINE. Empirical articles written in English that examined RCC advocacy service provision and/or impact in the US were included. The researchers reviewed abstracts and titles, and then full texts. Forty-five articles met criteria, were summarized, and double checked. Findings demonstrate advocacy is multi-faceted, beneficial, and challenging. Advocates work directly with survivors and interact with other responders on behalf of survivors. Specifically, advocates provide emotional support, safety plan, support survivors in making decisions, and assist them in navigating other systems. While advocates are generally regarded positively by survivors and responders, some responders have concerns about advocates. In addition, advocates sometimes report victim-blaming and being ill-equipped to meet survivors’ needs. Finally, advocates face specific challenges in their work with survivors and responders. Future research using diverse methodological approaches is needed to understand advocacy utilization and reach; survivors’ perceptions of advocacy; marginalized survivors’ experiences; connections between specific services, implementation, and outcomes; and effective strategies for advocates’ interactions with other responders. Additional resources to help advocates serve all survivors effectively and equitably; to support evaluator-practitioner partnerships; and to share unpublished data on advocacy may help contribute to improvements in advocacy practice.
Validating marker-less pose estimation with 3D x-ray radiography

To reveal the neurophysiological underpinnings of natural movement, neural recordings must be paired with accurate tracking of limbs and postures. Here we validate the accuracy of DeepLabCut (DLC) by comparing it to a 3D x-ray video radiography system that tracks markers placed under the skin (XROMM). We record behavioral data simultaneously with XROMM and high-speed video for DLC as marmosets engage in naturalistic foraging and reconstruct three-dimensional kinematics in a shared coordinate system. We find that DLC tracks position and velocity of 12 markers on the forelimb and torso with low median error (0.272 cm and 1.76 cm/s, respectively) corresponding to 2.9% of the full range of marker positions and 5.9% of the range of speeds. For studies that can accept this relatively small degree of error, DLC and similar marker-less pose estimation tools enable the study of more naturalistic, unconstrained behaviors in many fields including non-human primate motor control. Summary Statement We validate the accuracy of DeepLabCut, a widely used marker-less pose estimation tool, using a marker-based 3D x-ray radiography system (XROMM).
Physical exercise positively modulates nonalcoholic steatohepatitis‐related hepatic endoplasmic reticulum stress

Obesity is a predictive factor for the development of nonalcoholic steatohepatitis (NASH). Although some of the mechanisms associated with NASH development are still elusive, its pathogenesis relies on a complex broad spectrum of (interconnected) metabolic‐based disorders. We analyzed the effects of voluntary physical activity (VPA) and endurance training (ET), as preventive and therapeutic nonpharmacological strategies, respectively, against hepatic endoplasmic reticulum (ER) stress, ER‐related proapoptotic signaling, and oxidative stress in an animal model of high‐fat diet (HFD)‐induced NASH. Adult male Sprague–Dawley rats were divided into standard control liquid diet (SCLD) or HFD groups, with sedentary, VPA, and ET subgroups in both (sedentary animals with access to SCLD [SS], voluntarily physically active animals with access to SCLD [SV], and endurance‐trained animals with access to SCLD [ST] in the former and sedentary animals with access to liquid HFD [HS], voluntarily physically active animals with access to liquid HFD [HV], and endurance‐trained animals with access to liquid HFD [HT] in the latter, respectively). Hepatic ER stress and ER‐related proapoptotic signaling were evaluated by Western blot and reverse transcriptase‐polymerase chain reaction; redox status was evaluated through quantification of lipid peroxidation, protein carbonyls groups, and glutathione levels as well as antioxidant enzymes activity. In SCLD‐treated animals, VPA significantly decreased eukaryotic initiation factor‐2 alpha (eIF2α). In HFD‐treated animals, VPA significantly decreased eIF2α and phospho‐inositol requiring enzyme‐1 alpha (IRE1α) but ET significantly decreased eIF2α and significantly increased both spliced X‐box binding protein 1 (sXBP1) and unspliced X‐box binding protein 1; a significant increase of phosphorylated‐eIF2α (p‐eIF2α) to eIF2α ratio occurred in ET versus VPA. HS compared to SS disclosed a significant increase of total and reduced glutathione, HV compared to SV a significant increase of oxidized glutathione, HT compared to ST a significant increase of p‐eIF2α to eIF2α ratio and sXBP1. Physical exercise counteracts NASH‐related ER stress and its associated deleterious consequences through a positive and dynamical modulation of the hepatic IRE1α–X‐box binding protein 1 pathway.
Participation-based public art & design project model for culture-led urban regeneration

In recent years, public art has evolved to take a c ntral role in urban regeneration in public places and, more recently, has been integrat d into city branding. Published research and selected reports revealed that public art frequently opens up sensitive issues like social acceptance, ownership and cultur al relevance, and showed that a participation-based approach can be used to address th e issues. The literature review confirmed the main issues and the need for a theore tical platform to support future practice. The researcher has been a professional practitioner n the field for many years and was aware of the gap between contemporary practice and academic underpinning, resulting in divergent practices with unpredictable outcomes. Key elements found in the secondary research and overlaid with the fieldw ork experience of the researcher were combined to create an analytical tool to analy se 46 practical cases of public art and design. This revealed five invitation elements [triggers to induce participation] which were found to be connected to four participat ion elements [patterns of participation]. A first conceptual model was formul ated to investigate the interactions and transformation processes between these invitati on nd participation elements. The model was further developed and its validity was te ted through two distinctive action-based research projects in which the researc h r played a leading role. The projects confirmed the validity of the transformati on process in the model and emergent pragmatic value. Expert interviews confirm ed the validity of the model and understanding of how it may become sustainable, res ulting in a toolkit for implementation to engender debate in the academic a nd practitioner community. The final theoretical model offers new thinking for lea ding public art and design practitioners and related stakeholders, to achieve consistent add-value.
Pelvic elevation induces vertical kinetic energy without losing horizontal energy during running single-leg jump for distance

ABSTRACT In a running single-leg jump (RSLJ) for distance, the generation of vertical velocity without loss of horizontal velocity during the take-off phase is ideal, but difficult; however, we hypothesized that the pelvic rotation in the frontal plane achieved it. Here we show the effect of each segment rotation on the horizontal and vertical kinetic energies ( and ) of the centre of mass (CoM) during the take-off phase of an RSLJ for distance. We collected kinematic and ground-reaction-force data during RSLJs for distance by nine male long jumpers, involving an approximately 20-m approach in an outdoor field. We determined the components of the and changes due to each segment movement. Elevation of the pelvic free-leg side increased (0.53±0.16 J/kg, 9±3% of the total change). Pelvic axial rotation decreased , while pelvic elevation did not affect it (0.01±0.02 J/kg, no significant difference from zero). In contrast, forward rotations of the stance-leg shank and thigh decreased while simultaneously increasing . The results showed that pelvic elevation increased the vertical CoM velocity without causing a loss in horizontal velocity, although the lower-limb segments’ effects on the vertical and horizontal velocities exhibited a trade-off, as previously speculated. RSLJs for distance have been frequently assumed as sagittal movements. However, our findings highlight the importance of three-dimensional pelvic movement, particularly in the frontal plane, for controlling both the vertical and horizontal velocities. Highlights l We show the effect of each segment rotation on the horizontal and vertical kinetic energies ( and ) of the centre of mass during the take-off phase of a running single-leg jump for distance. l Elevation of the pelvic free-leg side increased but did not decrease , while the forward rotations of the stance-leg thigh and shank decreased , while simultaneously increasing . l We highlight the importance of pelvic movement in the frontal plane for controlling both the vertical and horizontal velocities with single-leg stance.
Anticholinergics and falls in older adults

ABSTRACT Introduction The use of medications with anticholinergic (ACh) properties is associated with numerous adverse effects especially in older adults. Emerging evidence suggests the presence of long-term effects with ACh use. Areas covered Our article presents an overview of ACh effects and falls in older individuals including examination of emerging evidence on ACh use and cumulative exposure on short-term and long-term falls risk. The databases CINAHL, MEDLINE, EMBASE, and Web of Science were searched for articles published from January 2002 to December 2021. Expert opinion Anticholinergic side effects include muscle weakness, blurred vision, and mental confusion which are likely to lead to increased falls risk. Many commonly used medications such as beta-blockers, calcium-channel blockers, and antihistamines are now known to have mild ACh properties. With polypharmacy now considered unavoidable in older patients, the cumulative effects of the use of multiple drugs with mild ACh properties may also lead to increased falls risk. The relationship between ACh drugs and falls may also be irreversible as ACh effects may extend beyond the period of drug use, due to cognitive and physical deconditioning following the initial exposure to ACh drugs. Future long-term studies with accurate quantification of cumulative ACh exposure and measurement of actual falls outcomes are urgently required.
Renal adaptation in relation to insectivorous feeding habit in the greater mouse‐eared bat, Myotis myotis (Chiroptera: Vespertilionidae)

We examined the histological structure of the kidneys of Myotis myotis to better understand their structural adaptations to dietary habits. M. myotis is an insectivorous bat species that belongs to the family Vespertilionidae . The kidneys of M. myotis are unilobular, bean‐shaped, and surrounded by a renal capsule. The two parts are distinguished by a thin cortex and a thicker medulla. Renal corpuscles consist of the glomerulus and Bowman's capsule. The proximal tubule consists of cubic cells with a well‐developed brush border, whereas the distal tubule is lined with a simple cubic epithelium without a brush border. The Henle's loop, located in the medullary region, was composed of flat cells. The microvilli of proximal tubule epithelial cells and basal lamina of proximal and distal tubule epithelial cells were periodic acid Schiff (PAS)‐positive. The PAS‐positive reaction of the microvilli of proximal tubular epithelial cells and basal lamina of proximal and distal tubule epithelial cells is due to the presence of glycogen, which may be used as an energy substrate during absorption. The presence of acidic glycoconjugates in the papilla was demonstrated by Alcian blue (pH 2.5)‐PAS staining. According to the result of silver impregnation staining, it was determined that reticular fibers form a dense fibrillary network in the kidney parenchymal tissue. Reticular fibers are responsible for demarcating and supporting the borders of cells by forming a thin network of fibrils beneath the basal lamina of the cells. Structural features in the kidney, such as a thin cortex and thicker medulla, long conical papilla, and division of the thick medulla into inner and outer regions, of M. myotis may be an adaptation to produce concentrated urine, thereby reducing water loss associated with insectivorous feeding habits.
Potential Candidates for Biomarkers in Bipolar Disorder: A Proteomic Approach through Systems Biology

Bipolar disorder (BD) is one of the most disabling diseases characterized by severe humor fluctuation. It is accompanied by cognitive and functional impairment in addiction to high suicide rates. BD is often underdiagnosed and treated incorrectly because many of the reported symptoms are not exclusive to the disorder. Once the diagnosis is exclusively clinical, it is not possible to state precisely. From that, proteomic approaches were used to identify, in a large scale, all proteins involved in cellular or tissue processes. This review aggregate data from blood proteomes, by using protein association network, of subjects with BD and healthy controls to suggest dysfunctional molecular pathways involved in disease. Original articles containing proteomic analysis were searched in PubMed. Seven studies were selected and data were extracted for posterior analysis. A protein-protein interaction network was created by STRING database. A final set of proteins in this network were employed as input in ClueGO and, the main biological process was visualized using R package pathview. The analysis revealed proteins associated with many biological processes, including growth and endocrine regulation, iron transportation, protease inhibition, protection against pathogens and cholesterol transport. Moreover, pathway analysis indicated the association of uncovered proteins with two main metabolic pathways: complement system and coagulation cascade. Thus, a better understanding on the pathophysiology of psychiatric disorders and the identification of potential biomarker candidates are essential to improve diagnostic, prognostic and design pharmacological strategies.
Interactions between Influenza A Virus Nucleoprotein and Gene Segment Untranslated Regions Facilitate Selective Modulation of Viral Gene Expression

Influenza A virus (IAV) is a respiratory pathogen that remains a significant source of morbidity and mortality. Escape from host immunity or emergence into new host species often requires mutations that modulate the functional activities of the viral glycoproteins hemagglutinin (HA) and neuraminidase (NA), which are responsible for virus attachment to and release from host cells, respectively. ABSTRACT The influenza A virus (IAV) genome is divided into eight negative-sense, single-stranded RNA segments. Each segment exhibits a unique level and temporal pattern of expression; however, the exact mechanisms underlying the patterns of individual gene segment expression are poorly understood. We previously demonstrated that a single substitution in the viral nucleoprotein (NP:F346S) selectively modulates neuraminidase (NA) gene segment expression while leaving other segments largely unaffected. Given what is currently known about NP function, there is no obvious explanation for how changes in NP can selectively modulate the replication of individual gene segments. In this study, we found that the specificity of this effect for the NA segment is virus strain specific and depends on the untranslated region (UTR) sequences of the NA segment. While the NP:F346S substitution did not significantly alter the RNA binding or oligomerization activities of NP in vitro, it specifically decreased the ability of NP to promote NA segment viral RNA (vRNA) synthesis. In addition to NP residue F346, we identified two other adjacent aromatic residues in NP (Y385 and F479) capable of similarly regulating NA gene segment expression, suggesting a larger role for this domain in gene-segment specific regulation. Our findings reveal a novel role for NP in selective regulation of viral gene segment replication and provide a framework for understanding how the expression patterns of individual viral gene segments can be modulated during adaptation to new host environments. IMPORTANCE Influenza A virus (IAV) is a respiratory pathogen that remains a significant source of morbidity and mortality. Escape from host immunity or emergence into new host species often requires mutations that modulate the functional activities of the viral glycoproteins hemagglutinin (HA) and neuraminidase (NA), which are responsible for virus attachment to and release from host cells, respectively. Maintaining the functional balance between the activities of HA and NA is required for fitness across multiple host systems. Thus, selective modulation of viral gene expression patterns may be a key determinant of viral immune escape and cross-species transmission potential. We identified a novel mechanism by which the viral nucleoprotein (NP) gene can selectively modulate NA segment replication and gene expression through interactions with the segment UTRs. Our work highlights an unexpected role for NP in selective regulation of expression from the individual IAV gene segments.
Validation of a Modified Version of Interpersonal Reactivity Index for Medical Students Using Rasch Analysis

Abstract Construct: Empathy has been accepted to interweave both cognitive aspects (the ability to put oneself in another person’s place), and affective (or emotional) aspects, indicating an emotional reaction or response to another person’s emotional state. Literature supports the positive influences of empathy on doctor-patient relationship, patient satisfaction, and positive clinical outcomes. Background: Many studies have dealt with the development of empathy measurement tools for physicians and medical students. A frequently used empathy measuring instrument for medical students is the “Interpersonal Reactivity Index” (IRI) which was designed to measure the multi-dimensional aspects of empathy in the general adult population. Most previous literature which validated IRI for medical students has used factor analysis, whilst studies applying Rasch models have been limited. Our study aimed to investigate the psychometric properties of a modified version of IRI for medical students using Rasch analysis. Approach: Medical students (1,293) from 15 medical schools in South Korea participated in an online questionnaire consisting of 28 items of the Korean translated version of IRI. We applied exploratory factor analysis (EFA) using polychoric correlation matrix to determine the optimal number of factors followed by Rasch analysis and McDonald’s Omega calculation. Findings: The adapted IRI-MS (IRI for medical students) consisted of 17 items in four dimensions: empathic concern (5), fictitious situation (4), perspective taking (4), and personal distress (4). The overall fit of IRI-MS revealed an acceptable goodness-of-fit for all 17 items and a positive point measure correlation for all items. Reliability indices from the Rasch modeling and McDonald’s Omega values of all four dimensions were satisfactory for research. We found the Wright-Andrich maps and category probability curves of the IRI’s four dimensions to be less than optimal in measuring empathy levels with adequate precision. Conclusions: Rasch analysis of IRI-MS fell short from being able to prove satisfactory validity in measuring the multidimensional nature of empathy in medical students. However, our study applying Rasch analysis may serve as groundwork for future studies, to further develop from the shortcomings of our findings. Supplemental data for this article are available online at at www.tandfonline. com/htlm .
Interactive and Reproducible Workflows for Exploring and Modeling RNA‐seq Data with pcaExplorer, Ideal, and GeneTonic

The generation and interpretation of results from transcriptome profiling experiments via RNA sequencing (RNA‐seq) can be a complex task. While raw data quality control, alignment, and quantification can be streamlined via efficient algorithms that can deliver the preprocessed expression matrix, a common bottleneck in the analysis of such large datasets is the subsequent in‐depth, iterative processes of data exploration, statistical testing, visualization, and interpretation. Specific tools for these workflow steps are available but require a level of technical expertise which might be prohibitive for life and clinical scientists, who are left with essential pieces of information distributed among different tabular and list formats.