Miracle-dz/UniFAR-FTUs huggingface dataset:
Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa

Pseudomonas aeruginosa causes hospital-borne infections in vulnerable patients, including immunocompromised individuals, burn victims, and cancer patients undergoing chemotherapy. There are no effective treatments for P. aeruginosa infections, which are usually broadly resistant to antibiotics. ABSTRACT Pseudomonas aeruginosa is a human pathogen that relies on quorum sensing to establish infections. The PqsE quorum-sensing protein is required for P. aeruginosa virulence factor production and infection. PqsE has a reported enzymatic function in the biosynthesis of the quorum-sensing autoinducer called PQS. However, this activity is redundant because, in the absence of PqsE, this role is fulfilled by alternative thioesterases. Rather, PqsE drives P. aeruginosa pathogenic traits via a protein-protein interaction with the quorum-sensing receptor/transcription factor RhlR, an interaction that enhances the affinity of RhlR for target DNA sequences. PqsE catalytic activity is dispensable for interaction with RhlR. Thus, the virulence function of PqsE can be decoupled from its catalytic function. Here, we present an immunoprecipitation-mass spectrometry method employing enhanced green fluorescent protein-PqsE fusions to define the protein interactomes of wild-type PqsE and the catalytically inactive PqsE(D73A) variant in P. aeruginosa and their dependence on RhlR. Several proteins were identified to have specific interactions with wild-type PqsE while not forming associations with PqsE(D73A). In the ΔrhlR strain, an increased number of specific PqsE interactors were identified, including the partner autoinducer synthase for RhlR, called RhlI. Collectively, these results suggest that specific protein-protein interactions depend on PqsE catalytic activity and that RhlR may prevent proteins from interacting with PqsE, possibly due to competition between RhlR and other proteins for PqsE binding. Our results provide a foundation for the identification of the in vivo PqsE catalytic function and, potentially, new proteins involved in P. aeruginosa quorum sensing. IMPORTANCE Pseudomonas aeruginosa causes hospital-borne infections in vulnerable patients, including immunocompromised individuals, burn victims, and cancer patients undergoing chemotherapy. There are no effective treatments for P. aeruginosa infections, which are usually broadly resistant to antibiotics. Animal models show that, to establish infection and to cause illness, P. aeruginosa relies on an interaction between two proteins, namely, PqsE and RhlR. There could be additional protein-protein interactions involving PqsE, which, if defined, could be exploited for the design of new therapeutic strategies to combat P. aeruginosa. Here, we reveal previously unknown protein interactions in which PqsE participates, which will be investigated for potential roles in pathogenesis.
Effect of roasting temperature and time on some physical and sensory properties of roasted chickpea (leblebi), and kinetic studies of the changes in leblebi color

The roasted chickpea, which locally called as leblebi, is a widely consumed traditional snack food in Turkey and Middle Eastern countries. Although leblebi is widely consumed, there is limited research on this product except some of its well-known properties. In this study, the change in texture and color of chickpeas during different roasting temperature and duration were determined. Based on preliminary studies, roasting conditions were selected as 150±5 °C, 180±5 °C and 200±5 °C and 4, 6, and 8 minutes. Textural changes were determined by the force deformation curves obtained from compression tests. Statistical analysis of the data indicated that when the roasting temperature and time increased, hardness of leblebi samples was decreased. During roasting process, L* values decreased meanwhile a* and b* values increased. When correlation coefficients (r) were taken into consideration, it was found that L* values followed zero-order reaction kinetics and had 36.81 kj/mol activation energy. Also, the a* and b* values resulted in first-order reaction kinetics and had 14.23 kj/mol and 11.21 kj/mol activation energy, respectively. The lowest value of speckling was determined at 150±5°C for 4 minutes, and the highest value was obtained at 200±5 °C for 8 minutes. Color without speckling was found to be significantly increasing when roasting time and temperature were increased. The lowest value of hardness was observed at 200±5 oC for 8 minutes of roasting.
The Critical Discourse Analysis on Novel Sabtu

This article aims to discuss an interesting phenomenon described in literacy media, the parts of fathers or man’s image in the narrative or story of the novel Sabtu Bersama Bapak (Saturday Together with Father) by Adhitya Mulya. This article uses the critical discourse analysis method from Norman Fairclough, such as text analysis with an analysis of patriarchal culture from Sylvia Walby. Further, the dominant father’s image is manifested from up to regulate the survival of all members of his family even though he (father) has died. Based on this, through the consumption of the text, namely the viewpoint of the researcher himself, the story in this novel represents a patriarchal ideology. The view of the image of men as the master of power and the image of women as controlled, in general, described in this novel through the position of wife and child in the dominance of patriarchal
Comment on the article "Existence and Regularity for an Energy Maximization Problem in Two Dimensi

A revised version of the last appendix of the (previous) paper "Existence and Regularity for an Energy Maximization Problem in Two Dimensions" by S.Kamvissis and E.A. Rakhmanov, that appeared in the Journal of Mathematical Physics, v.46, n.8, 2005. arXiv:0907.5571
Geometric measure of entanglement for symmetric states

Is the closest product state to a symmetric entangled multiparticle state also symmetric? This question has appeared in the recent literature concerning the geometric measure of entanglement. First, we show that a positive answer can be derived from results concerning symmetric multilinear forms and homogeneous polynomials, implying that the closest product state can be chosen to be symmetric. We then prove the stronger result that the closest product state to any symmetric multiparticle quantum state is necessarily symmetric. Moreover, we discuss generalizations of our result and the case of translationally invariant states, which can occur in spin models.
On the zeros of certain modular functions for the normalizers of congruence subgroups of low levels I

We research the location of the zeros of the Eisenstein series and the modular functions from the Hecke type Faber polynomials associated with the normalizers of congruence subgroups which are of genus zero and of level at most twelve. In Part I, we will consider the general theory of modular functions for the normalizers.
Geodetic Line at Constant Altitude above the Ellipsoid

The two-dimensional surface of a bi-axial ellipsoid is characterized by the lengths of its major and minor axes. Longitude and latitude span an angular coordinate system across. We consider the egg-shaped surface of constant altitude above (or below) the ellipsoid surface, and compute the geodetic lines - lines of minimum Euclidean length - within this surface which connect two points of fixed coordinates. This addresses the common "inverse" problem of geodesics generalized to non-zero elevations. The system of differential equations which couples the two angular coordinates along the trajectory is reduced to a single integral, which is handled by Taylor expansion up to fourth power in the eccentricity.
Critical percolation of virtually free groups and other tree-like graphs

This article presents a method for finding the critical probability pc for the Bernoulli bond percolation on graphs with the so-called tree-like structure. Such a graph can be decomposed into a tree of pieces, each of which has finitely many isomorphism classes. This class of graphs includes the Cayley graphs of amalgamated products, HNN extensions or general groups acting on trees. It also includes all transitive graphs with more than one end. The idea of the method is to find a multi-type Galton–Watson branching process (with a parameter p) which has finite expected population size if and only if the expected percolation cluster size is finite. This provides sufficient information about pc. In particular, if the pairwise intersections of pieces are finite, then pc is the smallest positive p such that det(M − 1) = 0, where M is the first-moment matrix of the branching process. If the pieces of the tree-like structure are finite, then pc is an algebraic number and we give an algorithm computing pc as a root of some algebraic function. We show that any Cayley graph of a virtually free group (i.e., a group acting on a tree with finite vertex stabilizers) with respect to any finite generating set has a tree-like structure with finite pieces. In particular, we show how to compute pc for the Cayley graph of a free group with respect to any finite generating set.
Effects of Benzo[]pyrene on DNA Damage and Histological alterations in Gonad of Scallop

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Effects of Benzo[]pyrene on DNA Damage and Histological alterations in Gonad of Scallop Miao Jing-Jing, Pan Lu-Qing, Liu Jing, Zhang Lin
The Effects of Decimalization on Return Volatility Components, Serial Correlation and Trading Costs

We examine the composition of return volatility, serial correlation, and trading costs before and after decimalization on the New York Stock Exchange. We decompose the variance of price changes into components associated with public news, rounding errors, and market-making frictions. We find that when stocks move from a fractional to a decimal trading system, the variance components due to market-making frictions and rounding errors decline significantly, whereas the component due to public news remains unchanged. The serial correlation of price changes weakens substantially after decimalization. The uninformed component of bid-ask spreads decreases significantly whereas the informed component has no significant change. JEL Classification: G1
Plant Science Today

Medicinal plants have been the major source of combating and treating infectious diseases and health disorders throughout the ages. To explore new sources of inexpensive and safe antioxidants and antibiotics. The antioxidant and antibacterial potential of different extracts Syzygium mytifolium (Roxb.) Walp. were evaluated through 1,1-Diphenyl-2-Picryl Hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), α-glucosidase inhibition, disk diffusion and agar well methods. The leaves, bark, root and whole plant of Syzygium mytifolium were extracted with various solvents viz ethanol, methanol and aqueous to give crude extracts, respectively. Among all, the ethanolic extract showed high antioxidant activity in terms of radical scavenging activity, reducing power and alpha-glucosidase inhibition at 1.84 μg/ml, 40.2 mmol/gm and 14.34 μg/ml, respectively. All the tested parts of the plant exhibited moderate to higher range of antibacterial activity against bacterial pathogens with the inhibition zones ranged between 2-27.8 mm. Syzygium mytifolium will serve as a very good source for the development of antioxidant supplements and antibiotics for addressing infectious pathogens and improvement of health status.
The effects of deficiencies of certain mineral nutrients on the activity and various properties of ribulose-1,5-diphosphate carboxylase from barley (Hordeum vulgare L.)

Barley plants grown in Mg, Mn, Zn and Cu deficient media were analyzed for specific activity, total activity and amount of RuDPCase present o Percent dry weight, as well as chlorophyll and soluble protein content were determinedo All deficiencies caused lower chlorophyll and RuDPCase con­ centrations than occurred in the control. Soluble protein content, however, was higher in all deficient plants than in the control, either on a dry or a fresh weight basiso 2+ The addition of 18 μM Mn 2+ increased the activity of Mg containing RuDPCase preparations as much as 133% above normal. The specific activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 60%, 118%, 159% and 149%, respec­ tively, of the controlo After 20 hours preincubation with Mg2+ , RuDPCase from Mg deficient plants attained 96% of the specific activity exhibited by the control. Total activity of RuDPCase from Mg, Mn, Zn and Cu deficient plants was 22%, 51%, 102% and 12 1%, respectively, of the control. Possible roles of Mn 2+ and Cu 2+ in the activation of RuDPCase :=ire disc.11SSPn.
Production and nutritional quality of black oat forage grown in different population stands under a successive cutting regime

The present study aimed to evaluate the agronomic productive and qualitative traits of forage of black oats grown in different stands of plants. The experimental design was a 2 x 2 factorial randomized complete block design consisting of four treatments, two plant stands (175 and 350 plants m) associated with two successive cutting periods (vegetative and full vegetative) and four replications. The stand of plants did not influence the production of dry biomass; with 175 plants m, we obtained a compensatory effect by the greater number of tillers m and/or tiller plant. The population stand influenced the chemical parameters, where 175 plants m resulted in forage with higher contents of hemicellulose and total digestible nutrients and a lower content of neutral detergent fiber. In the comparison between cuts, the first cut compared to the second presented higher values of crude protein (26.53%). It is recommended that the black oat be cultivated with a population stand of 175 plants m for generating an increase in the number of tillers m and tillers plant, in addition to improving the in situ dry matter digestibility of the whole plant, without causing reduction of production of dry biomass per unit area.
УДК 612.821-615.832.9:616.127 ON VALUE OF THE PSECHOLOGICAL SELF-REGULATION METHODS WITHIN COMPLEX THERAPY FOR CHRONIC PAIN PATIENTS

ON VALUE OF THE PSECHOLOGICAL SELF-REGULATION METHODS WITHIN COMPLEX THERAPY FOR CHRONIC PAIN PATIENTS DOI: 10.31618/ESU.2413-9335.2019. 7.66. 330 Ishinova V. A. PhD, medical psychologist, Department of Psychological Rehabilitation, FSBA « Federal Scientific Disabled Rehabilitation Center named after G.A. Albrecht, St. Petersburg Davidov A. T. Doctor of Medical Sciences, Professor, psychiatrist, FSBA «Federal Scientific Disabled Rehabilitation Center named after G.A. Albrecht, St. Petersburg
A Highly Selective and Compact Bandpass Filter with a Circular Spiral Inductor and an Embedded Capacitor Structure Using an Integrated Passive Device Technology on a GaAs Substrate

As one of the most commonly used devices in microwave systems, bandpass filters (BPFs) directly affect the performance of these systems. Among the processes for manufacturing filters, integrated passive device (IPD) technology provides high practicality and accuracy. Thus, to comply with latest development trends, a resonator-based bandpass filter with a high selectivity and a compact size, fabricated on a gallium arsenide (GaAs) substrate is developed. An embedded capacitor is connected between the ends of two divisions in a circular spiral inductor, which is intertwined to reduce its size to 0.024 λg × 0.013 λg with minimal loss, and along with the capacitor, it generates a center frequency of 1.35 GHz. The strong coupling between the two ports of the filter results in high selectivity, to reduce noise interference. The insertion loss and return loss are 0.26 dB and 25.6 dB, respectively, thus facilitating accurate signal propagation. The filter was tested to verify its high performance in several aspects, and measurement results showed good agreement with the simulation results.
Stability structures, motivic Donaldson-Thomas invariants and cluster transformations

We define new invariants of 3d Calabi-Yau categories endowed with a stability structure. Intuitively, they count the number of semistable objects with fixed class in the K-theory of the category ('number of BPS states with given charge' in physics language). Formally, our motivic DT-invariants are elements of quantum tori over a version of the Grothendieck ring of varieties over the ground field. Via the quasi-classical limit 'as the motive of affine line approaches to 1' we obtain numerical DT-invariants which are closely related to those introduced by Behrend. We study some properties of both motivic and numerical DT-invariants including the wall-crossing formulas and integrality. We discuss the relationship with the mathematical works (in the non-triangulated case) of Joyce, Bridgeland and Toledano-Laredo, as well as with works of physicists on Seiberg-Witten model (string junctions), classification of N=2 supersymmetric theories (Cecotti-Vafa) and structure of the moduli space of vector multiplets. Relating the theory of 3d Calabi-Yau categories with distinguished set of generators (called cluster collection) with the theory of quivers with potential we found the connection with cluster transformations and cluster varieties (both classical and quantum).
A gap in the essential spectrum of a cylindrical waveguide with a periodic aperturbation of the surface

It is proved that small periodic singular perturbation of a cylindrical waveguide surface may open a gap in the essential spectrum of the Dirichlet problem for the Laplace operator. If the perturbation period is long and the caverns in the cylinder are small, the gap certainly opens. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Electromagnetic 2-forms on space-time

Two field 2-forms on the space-time manifold, in a relationship of duality, are presented and included in the extended phase-space structure used to describe relativistic particles having both electric and magnetic charges. By exterior derivatives, these forms yield the two groups of Maxwell equations, while specific integrality conditions ensure magnetic monopole or electric charge quantization. Some properties of the common characteristic vector of the dual 2-forms are discussed. It is shown that the coupled energy-density continuity equation and the eikonal equation represent a classical, infinite-dimensional Hamiltonian system.
Contributions of Yersinia pestis outer membrane protein Ail to plague pathogenesis

Purpose of review Pathogenic Yersinia have been a productive model system for studying bacterial pathogenesis. Hallmark contributions of Yersinia research to medical microbiology are legion and include: (i) the first identification of the role of plasmids in virulence, (ii) the important mechanism of iron acquisition from the host, (iii) the first identification of bacterial surface proteins required for host cell invasion, (iv) the archetypical type III secretion system, and (v) elucidation of the role of genomic reduction in the evolutionary trajectory from a fairly innocuous pathogen to a highly virulent species. Recent findings The outer membrane (OM) protein Ail (attachment invasion locus) was identified over 30 years ago as an invasin-like protein. Recent work on Ail continues to provide insights into Gram-negative pathogenesis. This review is a synopsis of the role of Ail in invasion, serum resistance, OM stability, thermosensing, and vaccine development. Summary Ail is shown to be an essential virulence factor with multiple roles in pathogenesis. The recent adaptation of Yersinia pestis to high virulence, which included genomic reduction to eliminate redundant protein functions, is a model to understand the emergence of new bacterial pathogens.
Linear \infty-Harmonic maps between Rienmannian manifolds

In this paper, we give complete classifications of linear $\infty$-harmonic maps between Euclidean and Heisenberg spaces, between Nil and Sol spaces. We also classify all $\infty$-harmonic linear endomorphisms of Sol space and show that there is a subgroup of $\infty$-harmonic linear automorphisms in the group of linear automorphisms of Sol space.
Evaluation of sludge hydrogels resulting from the washing of sand and gravel as an alternative for the treatment of arid soils

En la fase de elaboración de materiales para la construcción, se genera una gran cantidad de residuos como el lodo generado en los procesos de lavado de los materiales agregados, originados de rocas, arena y gravas que no pueden ser reutilizados o aprovechados en los demás procesos de producción. En tal sentido, se busca crear alternativas de manejo y disposición de estos residuos. Para ello se requiere evaluar la fitotoxicidad de hidrogeles formulados a partir de lodos procedentes de las aguas resultantes del lavado de arena y piedra como alternativa para el tratamiento de suelos áridos y se evaluó la fitotoxicidad en los modelos biológicos Raphanus sativus y Brachiaria dictyoneura. De tal manera, para el estudio, se caracterizaron fisicoquímica, microbiológica y microscópicamente los lodos de lavado de arena y piedra. Los lodos fueron formulados tipo hidrogel para valorar la capacidad de retención y recuperación de agua, cinética de evaporación y fitotoxicidad. Los análisis realizados determinaron que el lodo de arena es un buen candidato para el tratamiento de suelos áridos, ya que este es óptimo para la germinación, promueve el crecimiento y desarrollo de las plantas como R. sativus y B. dictyoneura.
Generationand Immunohistological Characterizationof HumanMonoclonal

The purpose of this study was to identify human antibodies generated against autologous breast tumor cells by the host's immune response. Accordingly, lymphocytes from lymph nodes of seven different patients with metastatic breast carcinomas were immortalized by fusing them with a nonsecreting variant of murine myeloma cells. The screening for binding of antibodies to tumor cells was performed by indirect immunoperoxidase stain ing of paraffin-embedded tissue sections of the autologous tu mor. The selected hybrid cells, after being cloned three times, were stable for the secretion of immunoglobulins for over 2 years. A total of 81 human immunoglobulin-producing clones was obtained from an initial 595 wells with hybrid growth. Nine of these clones produced immunoglobulin M, none of which showed detectable binding to tissue antigens in breast. Seventytwo clones produced immunoglobulin G monoclonal antibodies, and 15 of these showed preferential binding to breast carcinoma cells. Three of these immunoglobulin G monoclonal antibodies were subjected to detailed immunohistological evaluations. Using these antibodies at concentrations ranging from 10 to 100 ng/ tissue section, the morphologically normal mammary epithelial cells could be discriminated from their malignant counterparts. The antibodies showed diffuse staining of cytoplasmic compo nents in the malignant counterparts. Under these conditions, lymphocytes, erythrocytes, and stromal cells in breast tissues were unstained. The antibodies showed variable reactivity with malignant epithelial cells of colon and stomach, and with normal epithelial cells lining the renal tubules and sebaceous glands in skin. Antigenic heterogeneity of malignant mammary epithelial cells was revealed. The antibodies may have value in the char acterization of tumor-associated antigens responsible for induc ing autologous immune responses.