Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of red algal bloom on growth and production of carps

An experiment was carried to assess the effect of red algal bloom on growth and production of carp, water quality and profit from carp for 120 days at Aquaculture Farm of Agriculture and Forestry University, Chitwan. The experiment included two treatments: carp polyculture in non-red pond and carp polyculture in red pond with algal bloom each with three replicates. Carp fingerlings were stocked at 1 fish/m2 and fed with pellet containing 24% CP at 3% body weight. Net yield of rohu was found significantly higher (p<0.05) in non-red ponds (0.38±0.01 t ha-1) than red ponds (0.24±0.05 t ha-1). Survival of rohu (84.9±1.4%), bighead (95.2±2.0%) and mrigal (88.1±14.4%) were also significantly higher (p<0.05) in non-red ponds than red ponds. Red algal bloom affected DO, nitrate and chlorophyll-a, nitrite, total nitrogen, total phosphorus, total dissolved solids and conductivity. However, overall carp production and profit from carp remained unaffected.
The existence of a fire‐mediated tree‐recruitment bottleneck in an Asian savanna

A considerable proportion of the global savanna biome has been mis‐classified as forest, especially in Asia. However, the structure and responses of dominant tree species to fire can help to clarify this ambiguity. Here, we examine demographic structure and fire responses of four dominant tree species in a deciduous dipterocarp forest (DDF) of the continental Southeast Asia. We examine whether fire creates a tree‐recruitment bottleneck in the DDF, as in savannas on other continents.
3D Perfusable Hydrogel Recapitulating the Cancer Dynamic Environment to in Vitro Investigate Metastatic Colonization

Metastasis is a dynamic process involving the dissemination of circulating tumor cells (CTCs) through blood flow to distant tissues within the body. Nevertheless, the development of an in vitro platform that dissects the crucial steps of metastatic cascade still remains a challenge. We here developed an in vitro model of extravasation composed of (i) a single channel-based 3D cell laden hydrogel representative of the metastatic site, (ii) a circulation system recapitulating the bloodstream where CTCs can flow. Two polymers (i.e., fibrin and alginate) were tested and compared in terms of mechanical and biochemical proprieties. Computational fluid-dynamic (CFD) simulations were also performed to predict the fluid dynamics within the polymeric matrix and, consequently, the optimal culture conditions. Next, once the platform was validated through perfusion tests by fluidically connecting the hydrogels with the external circuit, highly metastatic breast cancer cells (MDA-MB-231) were injected and exposed to physiological wall shear stress (WSS) conditions (5 Dyn/cm2) to assess their migration toward the hydrogel. Results indicated that CTCs arrested and colonized the polymeric matrix, showing that this platform can be an effective fluidic system to model the first steps occurring during the metastatic cascade as well as a potential tool to in vitro elucidate the contribution of hemodynamics on cancer dissemination to a secondary site.
Ectopic overexpression of a membrane-tethered transcription factor gene NAC60 from oilseed rape positively modulates programmed cell death and age-triggered leaf senescence.

Senescence is an integrative final stage of plant development that is governed by internal and external cues. The NAC (NAM, ATAF1/2, CUC2) transcription factor (TF) family is specific to plants and membrane-tethered NAC TFs (MTTFs) constitute a unique and sophisticated mechanism in stress responses and development. However, the function of MTTFs in oilseed rape (Brassica napus L.) remains unknown. Here, we report that BnaNAC60 is a MTTF associated with endoplasmic reticulum (ER) membrane. Expression of BnaNAC60 was induced during the progression of leaf senescence. Translocation of BnaNAC60 into nuclei was induced by ER stress and oxidative stress treatments. It binds to the NTLBS motif, rather than the canonical NAC recognition site (NACRS). Overexpression of BnaNAC60 devoid of transmembrane domain, but not the full-length BnaNAC60, induces significant ROS accumulation and hypersensitive response (HR)-like cell death in both tobacco and oilseed rape protoplasts. Moreover, ectopic overexpression BnaNAC60 devoid of transmembrane domain, but not the full-length BnaNAC60 in Arabidopsis also induces precocious leaf senescence. Furthermore, screening and expression profiling identified an array of functional genes that are significantly induced by BnaNAC60 expression. Further it was found that BnaNAC60 can activate the promoter activities of BnaNYC1, BnaRbohD, BnaBFN1, BnaZAT12 and multiple BnaVPEs in a dual-luciferase reporter assay. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation coupled to quantitative PCR (ChIP-qPCR) assays revealed that BnaNAC60 directly binds to the promoter regions of these downstream target genes. To summarize, our data show that BnaNAC60 is a MTTF that modulates cell death, ROS accumulation and leaf senescence.
Energy Harvesting Circuit with High RF-to-DC Conversion Efficiency

This paper presents an energy harvesting circuit for high efficiency performance. The proposed circuit consists of an RLC shunt resonance circuit integrated to a RF antenna. The resonance circuit is used to (i) selectively pick the desired signal whose output is fed to a Cockcroft-Walton voltage multiplier circuit; and (ii) match the impedance of the antenna with the Cockcroft-Walton voltage multiplier circuit for optimum power (DC) transfer. The proposed circuit exhibits an efficiency of 68% for an input power of $200\ \mu\mathrm{W}$.
End-of-Life-Care Quality in ICUs Is Associated With Family Surrogates’ Severe Anxiety and Depressive Symptoms During Their First 6 Months of Bereavement*

OBJECTIVES: Evidence linking end-of-life-care quality in ICUs to bereaved family members’ psychologic distress remains limited by methodological insufficiencies of the few studies on this topic. To examine comprehensively the associations of family surrogates’ severe anxiety and depressive symptoms with end-of-life-care quality in ICUs over their first 6 months of bereavement. DESIGN: Prospective, longitudinal, observational study. SETTING/PARTICIPANTS: Family surrogates (n = 278) were consecutively recruited from seven medical ICUs at two academically affiliated medical centers in Taiwan. MEASUREMENTS AND STATISTICAL ANALYSIS: Family surrogates’ anxiety and depressive symptoms were assessed 1, 3, and 6 months postloss using the Hospital Anxiety and Depression Scale. Family satisfaction with end-of-life care in ICUs was assessed 1-month postloss by the Family Satisfaction in the ICU questionnaire. Patients’ end-of-life care was documented over the patient’s ICU stay. Associations of severe anxiety and depressive symptoms (scores ≥ 8 for each subscale) with end-of-life-care quality in ICUs (documented by patient care received and family satisfaction with end-of-life care in ICUs) were examined by multivariate logistic regression models with generalized estimating equation. MAIN RESULTS: Prevalence of severe anxiety and depressive symptoms decreased significantly over time. Surrogates’ lower likelihood of severe anxiety or depressive symptoms 3–6 month postloss was associated with death without cardiopulmonary resuscitation, withdrawing life-sustaining treatments, and higher family satisfaction with end-of-life care in ICUs. Bereaved surrogates’ higher likelihood of these symptoms was associated with physician-surrogate prognostic communication and conducting family meetings before patients died. CONCLUSIONS: End-of-life-care quality in ICUs is associated with bereaved surrogates’ psychologic well-being. Enhancing end-of-life-care quality in ICUs by improving the process of end-of-life care, for example, promoting death without cardiopulmonary resuscitation, withdrawing life-sustaining treatments, and increasing family satisfaction with end-of-life care, can lighten bereaved family surrogates’ severe anxiety symptoms and severe depressive symptoms.
NMR and Computational Studies as Analytical and High-Resolution Structural Tool for Complex Hydroperoxides and Diastereomeric Endo-Hydroperoxides of Fatty Acids in Solution-Exemplified by Methyl Linolenate

A combination of selective 1D Total Correlation Spectroscopy (TOCSY) and 1H-13C Heteronuclear Multiple Bond Correlation (HMBC) NMR techniques has been employed for the identification of methyl linolenate primary oxidation products without the need for laborious isolation of the individual compounds. Complex hydroperoxides and diastereomeric endo-hydroperoxides were identified and quantified. Strongly deshielded C–O–O–H 1H-NMR resonances of diastereomeric endo-hydroperoxides in the region of 8.8 to 9.6 ppm were shown to be due to intramolecular hydrogen bonding interactions of the hydroperoxide proton with an oxygen atom of the five-member endo-peroxide ring. These strongly deshielded resonances were utilized as a new method to derive, for the first time, three-dimensional structures with an assignment of pairs of diastereomers in solution with the combined use of 1H-NMR chemical shifts, Density Functional Theory (DFT), and Our N-layered Integrated molecular Orbital and molecular Mechanics (ONIOM) calculations.
The Effect of Emotional and Cultural Intelligences on Networks’ Behaviors in International SMEs: Evidence from Portugal

The major purpose of this research was to study the predictive value of the top managers’ psychological characteristics regarding their networking behavior. In the international business management context of small- and medium-sized enterprises, we took the top managers’ cultural intelligence and emotional intelligence as determinant capabilities to perform better in their external networking. The sample was composed of 307 Portuguese SMEs’ international decision-makers, specifically founders, owners, chief executive officers (CEOs), managers of international activities, international market managers, or commercial managers. The data was collected from 2–30 April 2019 through online surveys directed to the Portuguese decision-makers that were directly responsible for the firms’ international activities. As a data collection instrument, the surveys were pretested and sent by e-mail. The average age of the participants was approximately 50 years old for males and 45 years old for females. We used self-reported measures to assess the different constructs and the hierarchical regression analysis to test our hypotheses. The results showed that cultural intelligence and emotional intelligence were significant drivers of decision-makers’ external networking behavior. A new factor structure concerning external networking behavior was retained. The major results exhibited the predictive value of some cultural and emotional intelligence dimensions over the new retained external networking behavior factors. Therefore, in the international business management context, the capability to adapt to new cultural contexts, as well as the capability to reason about emotions, improved the international decision-makers’ external networking behavior.
Proteomic analysis of mitochondrial biogenesis in cardiomyocytes differentiated from human induced pluripotent stem cells.

Mitochondria play key roles in the differentiation and maturation of human cardiomyocytes (CMs). As human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold potential in the treatment of heart diseases, we sought to identify key mitochondrial pathways and regulators, which may provide targets for improving cardiac differentiation and maturation. Proteomic analysis was performed on enriched mitochondrial protein extracts isolated from hiPSC-CMs differentiated from dermal fibroblasts (dFCM) and cardiac fibroblasts (cFCM) at time points between 12 and 115 days of differentiation, and from adult and neonatal mouse hearts. Mitochondrial proteins with a 2-fold change at time points up to 120 days relative to 12 days were subjected to Ingenuity Pathway Analysis (IPA). The highest upregulation was in metabolic pathways for fatty acid oxidation (FAO), the tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS) and branched chain amino acid (BCAA) degradation. The top upstream regulators predicted to be activated were peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1-α), the insulin receptor (IR) and the retinoblastoma protein (Rb1) transcriptional repressor. IPA and immunoblotting showed upregulation of the mitochondrial LonP1 protease - a regulator of mitochondrial proteostasis, energetics and metabolism. LonP1 knockdown increased FAO in neonatal rat ventricular cardiomyocytes (nRVMs). Our results support the notion that LonP1 upregulation negatively regulates FAO in cardiomyocytes to calibrate the flux between glucose and fatty acid oxidation. We discuss potential mechanisms by which IR, Rb1 and LonP1 regulate the metabolic shift from glycolysis to OXPHOS and FAO. These newly identified factors and pathways may help in optimizing the maturation of iPSC-CMs.
Phosphomatics: interactive interrogation of substrate-kinase networks in global phosphoproteomics datasets

MOTIVATION Mass spectrometry-based phosphoproteomics can routinely identify and quantify thousands of phosphorylated peptides from a single experiment. However interrogating possible upstream kinases and identifying key literature for phosphorylation sites is laborious and time-consuming. RESULTS Here, we present Phosphomatics - a publicly available web resource for interrogating phosphoproteomics data. Phosphomatics allows researchers to upload phosphoproteomics data and interrogate possible relationships from a substrate-, kinase-, or pathway-centric viewpoint. AVAILABILITY Phosphomatics is freely available via the internet at: https://phosphomatics.com. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
A minimally invasive catheterization of the external jugular vein in suckling piglets using ultrasound guidance

There is a growing interest for minimally invasive surgical procedures to improve experimental animal welfare. Minimally invasive catheterization procedures in pigs have been already developed using Seldinger technique but reproducibility is low, especially in young pigs. A novel method for a minimally invasive catheterization of external jugular vein was evaluated in suckling piglets of 21 days of age. Growth performance and haptoglobin concentration in plasma were measured throughout a four-week study in a group of seven catheterized piglets and a group of seven non-catheterized piglets. Catheterization was performed using Seldinger technique under continuous ultrasound monitoring for vein detection and needle insertion. The surgical procedure was quick and showed a great reproducibility. All catheters remained functional during the first week after catheterization. Catheterization in piglets did not significantly affect body weight (BW) and feed intake during four weeks after the surgical intervention compared to non-catheterized piglets (P > 0.10). Haptoglobin concentration in plasma was greater in catheterized piglets compared with non-catheterized piglets, with a significant increase over two weeks after catheter insertion (P < 0.05), suggesting the development of a chronic inflammation in catheterized piglets. This method can be easily performed in piglets with minimal effect on growth and feeding behaviour. Transposition to heavier pigs should be considered.
Affective touch experiences across the lifespan: Development of the Tactile Biography questionnaire and the mediating role of attachment style

The sense of touch develops early in life and becomes a determinant aspect of our personal narratives, providing crucial information about the world around us and playing a prominent role in affective and social interactions. In this study we aimed to explore whether individual differences in touch experiences across the lifespan are related to adult attachment styles and to perceived touch deprivation. For this we first developed an instrument, namely the Tactile Biography, to quantify individual differences in affective touch experiences throughout life. Secondly, we performed a set of regressions models and a mediation analysis to investigate the role of attachment in relation to both the tactile history and perceived touch deprivation. We found that experiences of affective touch during childhood and adolescence seem to be closely associated with adult attachment styles and adult social touch experiences. Avoidant attachment appears to serve as a mediator in the relationship between earlier (childhood/adolescent) and later (adult) affective touch experiences, as well as between earlier affective touch experiences and perceived touch deprivation. These findings offer further support to existing literature, providing novel insights for the fields of social affective touch and attachment research.
Newly Synthesized Fluorinated Cinnamylpiperazines Possessing Low In Vitro MAO-B Binding

Herein, we report on the synthesis and pharmacological evaluation of ten novel fluorinated cinnamylpiperazines as potential monoamine oxidase B (MAO-B) ligands. The designed derivatives consist of either cinnamyl or 2-fluorocinnamyl moieties connected to 2-fluoropyridylpiperazines. The three-step synthesis starting from commercially available piperazine afforded the final products in overall yields between 9% and 29%. An in vitro competitive binding assay using l-[3H]Deprenyl as radioligand was developed and the MAO-B binding affinities of the synthesized derivatives were assessed. Docking studies revealed that the compounds 8–17 were stabilized in both MAO-B entrance and substrate cavities, thus resembling the binding pose of l-Deprenyl. Although our results revealed that the novel fluorinated cinnamylpiperazines 8–17 do not possess sufficient MAO-B binding affinity to be eligible as positron emission tomography (PET) agents, the herein developed binding assay and the insights gained within our docking studies will certainly pave the way for further development of MAO-B ligands.
A data-driven characterisation of natural facial expressions when giving good and bad news

Facial expressions carry key information about an individual’s emotional state. Research into the perception of facial emotions typically employs static images of a small number of artificially posed expressions taken under tightly controlled experimental conditions. However, such approaches risk missing potentially important facial signals and within-person variability in expressions. The extent to which patterns of emotional variance in such images resemble more natural ambient facial expressions remains unclear. Here we advance a novel protocol for eliciting natural expressions from dynamic faces, using a dimension of emotional valence as a test case. Subjects were video recorded while delivering either positive or negative news to camera, but were not instructed to deliberately or artificially pose any specific expressions or actions. A PCA-based active appearance model was used to capture the key dimensions of facial variance across frames. Linear discriminant analysis distinguished facial change determined by the emotional valence of the message, and this also generalised across subjects. By sampling along the discriminant dimension, and back-projecting into the image space, we extracted a behaviourally interpretable dimension of emotional valence. This dimension highlighted changes commonly represented in traditional face stimuli such as variation in the internal features of the face, but also key postural changes that would typically be controlled away such as a dipping versus raising of the head posture from negative to positive valences. These results highlight the importance of natural patterns of facial behaviour in emotional expressions, and demonstrate the efficacy of using data-driven approaches to study the representation of these cues by the perceptual system. The protocol and model described here could be readily extended to other emotional and non-emotional dimensions of facial variance.
Concordance of Paraphilic Interests and Behaviors

ABSTRACT We examined the concordance of paraphilic interests and behaviors across 13 themes in an online sample of 1,036 men and women. Paraphilic interests were significantly and positively correlated with behaviors across all 13 themes. Associations were strongest for masochism and sadism, and weakest for pedohebephilia and frotteurism. Paraphilic interest and behavior were significantly and positively correlated after accounting for gender and sexual orientation. Moderated moderation analysis was significant for five themes. Gender was a moderator for eroticized gender, but only among heterosexual participants, where concordance was higher for heterosexual men than for heterosexual women. For both exhibitionism and frotteurism, gender was a significant moderator, but only for nonheterosexual participants, where concordance was stronger for nonheterosexual men than for nonheterosexual women. For pedohebephilia, interest was significantly associated with behavior for heterosexual men, heterosexual women, and nonheterosexual men, but not for nonheterosexual women. For zoophilia, there was a significant association between interest and behavior for heterosexual men, nonheterosexual men, and nonheterosexual women, but not heterosexual women. Additional analyses found sex drive moderated the positive associations of 7 of 13 paraphilic themes, with 5 of these 7 showing the expected effect of higher concordance of interests and behaviors at higher levels of sex drive.
Trabecular Bone is Increased in a Rat Model of Polycystic Ovary Syndrome

Abstract Polycystic ovary syndrome (PCOS) in an intricate disorder characterized by reproductive and metabolic abnormalities that may affect bone quality and strength along with the lifespan. The present study analysed the impact of postnatal androgenization (of a single dose of testosterone propionate 1.25 mg subcutaneously at day 5 of life) on bone development and markers of bone metabolism in adult female Wistar rats. Compared with healthy controls, the results of measurements of micro-computed tomography (microCT) of the distal femur of androgenized rats indicated an increased cortical bone volume voxel bone volume to total volume (VOX BV/TV) and higher trabecular number (Tb.n) with reduced trabecular separation (Tb.sp). A large magnitude effect size was observed in the levels of circulating bone formation Procollagen I N-terminal propeptide (P1NP) at day 60 of life; reabsorption cross-linked C-telopeptide of type I collagen (CTX) markers were similar between the androgenized and control rats at days 60 and 110 of life. The analysis of gene expression in bone indicated elements for an increased bone mass such as the reduction of the Dickkopf-1 factor (Dkk1) a negative regulator of osteoblast differentiation (bone formation) and the reduction of Interleukin 1-b (Il1b), an activator of osteoclast differentiation (bone reabsorption). Results from this study highlight the possible role of the developmental programming on bone microarchitecture with reference to young women with PCOS.