Miracle-dz/UniFAR-FTUs huggingface dataset:
Context-dependent reproductive site choice in a Neotropical frog

In organisms whose offspring develop in discrete habitat patches such as pools, studies have frequently shown that adults avoid sites based on a single risk factor facing offspring. However, natural reproductive sites often vary in multiple risk factors in both space and time. In this study, I used choice tests among field mesocosms to determine whether adults of a Neotropical anuran, Edalorhina perezi, select the safest pools for offspring based on two biotic risks of different magnitude: insect predators and conspecific competitors. I also investigated whether adult site-choice was context dependent (i.e., whether it varied by sex or by season or whether it was based on local pool quality). I found that both sexes avoided pools containing predatory insects, but only females significantly avoided those containing conspecific tadpoles. When offered two risky options, both sexes favored pools with competitors over those with predators. Site-choice behavior also varied depending on the temporal and spatial context. Female sensitivity to insect predators decreased late in the season. In addition, both sexes exhibited dampened reproductive activity when only risky sites were available locally. This study emphasizes that social and environmental factors simultaneously impact reproductive site choice. Whether a site is accepted ultimately depends not only on the assessment of mortality risks to offspring but also on the sex and spatiotemporal context of the decision maker. Copyright 2003.
The Mediating Effects of Marital Intimacy and Work Satisfaction in the Relationship between Husbands’ Domestic Labor and Depressive Mood of Married Working Women

The purpose of the study was to examine the relationships between the husbands’ domestic labor and marital intimacy, work satisfaction, and depressive mood in married working women. We used the sixth (2016) dataset from the Women and Families Panel Survey conducted by the Korean Women’s Development Institute (KWDI). The subjects were 791 married working women who lived with a wage-earner husband and who did not have a housework assistant. The correlations between variables were measured and the fit of the structural equation model was assessed. We used a mediation model in which the husbands’ domestic labor affected the depressive mood of married working women through mediation of marital intimacy and work satisfaction. Bootstrapping was used to verify the significance of the indirect effects of the mediating variables. Husbands’ domestic labor had a significant effect on married women’s marital intimacy and work satisfaction, but no significant direct effect on depressive mood. Marital intimacy had a significant effect on work satisfaction, and these two variables were significantly related to reductions in the depressive mood score. Husbands’ domestic labor was found to be a complete mediator of depressive mood through its effects on marital intimacy and work satisfaction. Husbands’ domestic labor did not directly reduce married working women’s depressive mood scores, but instead reduced them indirectly through effects on marital intimacy and work satisfaction.
Glioma induced alterations in fecal short-chain fatty acids and neurotransmitters

Aim: To explore fecal short-chain fatty acids and neurotransmitter alterations in a mouse–glioma model and glioma patients. Methods: Liquid chromatography–mass spectrometry and 16S rRNA-sequencing from fecal samples were performed to measure metabolite levels and taxa abundance in mice/humans. Mice underwent GL261 implantation with/without temozolomide. Glioma patients were compared with healthy controls. Results: Glioma altered several short-chain fatty acids and neurotransmitter levels. Reduced 5-hydroxyindoleaceic acid and norepinephrine levels were seen in mice and humans. Interestingly, temozolomide treatment abrogates the effects of glioma on fecal metabolites. Conclusion: Our findings demonstrate the interplay between glioma and the gut–brain axis. Further work is required to identify pathways within the gut–brain axis by which glioma influences and promotes the modulation of fecal metabolites and microbiome.
Soluble SORLA Enhances Neurite Outgrowth and Regeneration through Activation of the EGF Receptor/ERK Signaling Axis

SORLA is a transmembrane trafficking protein associated with Alzheimer's disease risk. Although SORLA is abundantly expressed in neurons, physiological roles for SORLA remain unclear. Here, we show that cultured transgenic neurons overexpressing SORLA feature longer neurites, and accelerated neurite regeneration with wounding. Enhanced release of a soluble form of SORLA (sSORLA) is observed in transgenic mouse neurons overexpressing human SORLA, while purified sSORLA promotes neurite extension and regeneration. Phosphoproteomic analyses demonstrate enrichment of phosphoproteins related to the epidermal growth factor (EGFR)/ERK pathway in SORLA transgenic mouse hippocampus from both genders. sSORLA coprecipitates with EGFR in vitro, and sSORLA treatment increases EGFR Y1173 phosphorylation, which is involved in ERK activation in cultured neurons. Furthermore, sSORLA triggers ERK activation, whereas pharmacological EGFR or ERK inhibition reverses sSORLA-dependent enhancement of neurite outgrowth. In search for downstream ERK effectors activated by sSORLA, we identified upregulation of Fos expression in hippocampus from male mice overexpressing SORLA by RNAseq analysis. We also found that Fos is upregulated and translocates to the nucleus in an ERK-dependent manner in neurons treated with sSORLA. Together, these results demonstrate that sSORLA is an EGFR-interacting protein that activates EGFR/ERK/Fos signaling to enhance neurite outgrowth and regeneration. SIGNIFICANCE STATEMENT SORLA is a transmembrane trafficking protein previously known to reduce the levels of amyloid-β, which is critical in the pathogenesis of Alzheimer's disease. In addition, SORLA mutations are a risk factor for Alzheimer's disease. Interestingly, the SORLA ectodomain is cleaved into a soluble form, sSORLA, which has been shown to regulate cytoskeletal signaling pathways and cell motility in cells outside the nervous system. We show here that sSORLA binds and activates the EGF receptor to induce downstream signaling through the ERK serine/threonine kinase and the Fos transcription factor, thereby enhancing neurite outgrowth. These findings reveal a novel role for sSORLA in promoting neurite regeneration through the EGF receptor/ERK/Fos pathway, thereby demonstrating a potential neuroprotective mechanism involving SORLA.
Coinhibition of activated p38 MAPKα and mTORC1 potentiates stemness maintenance of HSCs from SR1‐expanded human cord blood CD34 + cells via inhibition of senescence

The stemness of ex vivo expanded hematopoietic stem cells (HSCs) is usually compromised by current methods. To explore the failure mechanism of stemness maintenance of human HSCs, which were expanded from human umbilical cord blood (hUCB) CD34+ cells, by differentiation inhibitor Stem Regenin 1 (SR1), an antagonist of aryl hydrocarbon receptor, we investigated the activity of p38 mitogen‐activated protein kinase α (p38 MAPKα, p38α) and mammalian target of rapamycin complex 1 (mTORC1), and their effect on SR1‐expanded hUCB CD34+ cells. Our results showed that cellular senescence occurred in the SR1‐expanded hUCB CD34+ cells in which p38α and mTORC1 were successively activated. Furthermore, their coinhibition resulted in a further decrease in hUCB CD34+ cell senescence without an effect on apoptosis, promoted the maintenance of expanded phenotypic HSCs without differentiation inhibition, increased the hematopoietic reconstitution ability of multiple lineages, and potentiated the long‐term self‐renewal capability of HSCs from SR1‐expanded hUCB CD34+ cells in NOD/Shi‐scid/IL‐2Rγnull mice. Our mechanistic study revealed that senescence inhibition by our strategy was mainly attributed to downregulation of the splicesome, proteasome formation, and pyrimidine metabolism signaling pathways. These results suggest that coinhibition of activated p38α and mTORC1 potentiates stemness maintenance of HSCs from SR1‐expanded hUCB CD34+ cells via senescence inhibition. Thus, we established a new strategy to maintain the stemness of ex vivo differentiation inhibitor‐expanded human HSCs via coinhibition of multiple independent senescence initiating signal pathways. This senescence inhibition‐induced stemness maintenance of ex vivo expanded HSCs could also have an important role in other HSC expansion systems.
Serum C-reactive protein to albumin ratio and mortality associated with peritoneal dialysis

Abstract Background Serum C-reactive protein to albumin ratio (CAR) was recently identified as a poor marker of prognosis among various populations. The current study aimed to examine the association between CAR and all-cause mortality among patients undergoing peritoneal dialysis (PD). Methods A total of 758 patients with PD were included in this study during the period from 1 November 2005 to 28 February 2017 and followed up until 31 May 2017. The primary outcome was all-cause mortality. We used multivariate Cox proportional hazard models and Kaplan-Meier survival curves to assess the relationship between CAR and all-cause mortality in these patients. Results Among 758 participants, mean age was 49.1 ± 14.2 years, with 56% males and 18.6% prevalence of diabetes. Median CAR was 0.13 (interquartile range [IQR], 0.07–0.34). After 27 months (IQR, 14–40 months) of follow-up, 157 deaths had been reported. After adjusting for confounding factors, we found a significant association between serum CAR and all-cause mortality among those in the highest CAR group (hazard ratio 1.91, 95% confidence interval 1.05– 3.47, p = 0.034). Conclusions In patients undergoing PD, an increase in serum CAR is independently associated with increased risk for all-cause mortality.
Efficacy of an Internet-Based Program to Promote Physical Activity and Exercise after Inpatient Rehabilitation in Persons with Multiple Sclerosis: A Randomized, Single-Blind, Controlled Study

Background: Multimodal rehabilitation improves fatigue and mobility in persons with multiple sclerosis (PwMS). Effects are transient and may be conserved by internet-based physical activity promotion programs. Objective: Evaluate the effects of internet-based physical activity and exercise promotion on fatigue, quality of life, and gait in PwMS after inpatient rehabilitation. Methods: PwMS (Expanded Disability Status Scale (EDSS) ≤ 6.0, fatigue: Würzburg Fatigue Inventory for Multiple Sclerosis (WEIMuS) ≥ 32) were randomized into an intervention group (IG) or a control group (CG). After rehabilitation, IG received 3 months of internet-based physical activity promotion, while CG received no intervention. Primary outcome: self-reported fatigue (WEIMuS). Secondary outcomes: quality of life (Multiple Sclerosis Impact Scale 29, MSIS-29), gait (2min/10m walking test, Tinetti score). Measurements: beginning (T0) and end (T1) of inpatient rehabilitation, 3 (T2) and 6 (T3) months afterwards. Results: 64 of 84 PwMS were analyzed (IG: 34, CG: 30). After rehabilitation, fatigue decreased in both groups. At T2 and T3, fatigue increased again in CG but was improved in IG (p < 0.001). MSIS-29 improved in both groups at T1 but remained improved at T2 and T3 only in IG. Gait improvements were more pronounced in IG at T2. Conclusions: The study provides Class II evidence that the effects of rehabilitation on fatigue, quality of life, and gait can be maintained for 3–6 months with an internet-based physical activity and exercise promotion program.
A Roadmap for the Success of Oncolytic Parvovirus-Based Anticancer Therapies.

Autonomous rodent protoparvoviruses (PVs) are promising anticancer agents due to their excellent safety profile, natural oncotropism, and oncosuppressive activities. Viral infection can trigger immunogenic cell death, activating the immune system against the tumor. However, the efficacy of this treatment in recent clinical trials is moderate compared with results seen in preclinical work. Various strategies have been employed to improve the anticancer activities of oncolytic PVs, including development of second-generation parvoviruses with enhanced oncolytic and immunostimulatory activities and rational combination of PVs with other therapies. Understanding the cellular factors involved in the PV life cycle is another important area of investigation. Indeed, these studies may lead to the identification of biomarkers that would allow a more personalized use of PV-based therapies. This review focuses on this work and the challenges that still need to be overcome to move PVs forward into clinical practice as an effective therapeutic option for cancer patients. Expected final online publication date for the Annual Review of Virology, Volume 7 is September 29, 2020. Please see http://www.annualreviews. org/page/journal/pubdates for revised estimates.
Galvanic Redox Potentiometry-Based Microelectrode Array for Synchronous Ascorbate and Single-Unit Recordings in Rat Brain.

Neuronal communication relies on the cooperation between the chemical and electrical patterns of neurons. Thus, techniques for illustrating the linkage of the neurochemical events and action potentials with high temporal and spatial resolution is imperative to gain a comprehensive understanding of the intricacies of brain function. Herein, we integrate galvanic redox potentiometry (GRP) and electrophysiological recording onto a 16-site Au microelectrode array (MEA), one of which for indicating ascorbate concentration while the rest of Au sites for single-unit activity assessment. The electrochemical probing site was modified with single-walled carbon nanotubes (SWNTs) to promote electron-transfer kinetics of ascorbate at low overpotential so as to enlarge the driving force for the spontaneous ascorbate/O2 cell reaction. The resulting GRP-based MEA outputs open-circuit potential that is in linear relationship of the logarithmic ascorbate concentration and exhibits high selectivity against a set of coexisting electroactive species. Furthermore, no reciprocal interference between the two recording systems is observed during concurrent GRP sensing of ascorbate and single-unit recording in rat brain. In vivo feasibility of the GRP-based MEA is demonstrated by synchronous real-time measurement of ascorbate release and electrical activity from multiple neuronal populations during spreading depression (SD). Our GRP-based MEA sensor creates new opportunities to realize high-throughput screening or mapping of neurochemical patterns in a larger dimension and correlate them to neuron functions across spatial scale.
Cryogenic OrbiSIMS Localizes Semi‐Volatile Molecules in Biological Tissues

OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. Here we report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS decreases ion-beam induced fragmentation, allowing large molecules such as triglycerides to be more reliably identified. It also increases chemical coverage to include biomolecules with intermediate or high vapor pressures, such as free fatty acids and semi-volatile organic compounds (SVOCs). We find that Cryo-OrbiSIMS reveals the hitherto unknown localization patterns of SVOCs with high spatial and chemical resolution in diverse plant, animal and human tissues. We also show that Cryo-OrbiSIMS can be combined with genetic analysis to identify enzymes regulating SVOC metabolism. Cryo-OrbiSIMS is applicable to high resolution imaging of a wide variety of non-volatile and semi-volatile molecules across many areas of biomedicine.
Orphan G Protein Coupled Receptors in Affective Disorders

G protein coupled receptors (GPCRs) are the main mediators of signal transduction in the central nervous system. Therefore, it is not surprising that many GPCRs have long been investigated for their role in the development of anxiety and mood disorders, as well as in the mechanism of action of antidepressant therapies. Importantly, the endogenous ligands for a large group of GPCRs have not yet been identified and are therefore known as orphan GPCRs (oGPCRs). Nonetheless, growing evidence from animal studies, together with genome wide association studies (GWAS) and post-mortem transcriptomic analysis in patients, pointed at many oGPCRs as potential pharmacological targets. Among these discoveries, we summarize in this review how emotional behaviors are modulated by the following oGPCRs: ADGRB2 (BAI2), ADGRG1 (GPR56), GPR3, GPR26, GPR37, GPR50, GPR52, GPR61, GPR62, GPR88, GPR135, GPR158, and GPRC5B.
Diabetes Self-Management in the Age of Social Media: Large-Scale Analysis of Peer Interactions Using Semiautomated Methods

Background Online communities have been gaining popularity as support venues for chronic disease management. User engagement, information exposure, and social influence mechanisms can play a significant role in the utility of these platforms. Objective In this paper, we characterize peer interactions in an online community for chronic disease management. Our objective is to identify key communications and study their prevalence in online social interactions. Methods The American Diabetes Association Online community is an online social network for diabetes self-management. We analyzed 80,481 randomly selected deidentified peer-to-peer messages from 1212 members, posted between June 1, 2012, and May 30, 2019. Our mixed methods approach comprised qualitative coding and automated text analysis to identify, visualize, and analyze content-specific communication patterns underlying diabetes self-management. Results Qualitative analysis revealed that “social support” was the most prevalent theme (84.9%), followed by “readiness to change” (18.8%), “teachable moments” (14.7%), “pharmacotherapy” (13.7%), and “progress” (13.3%). The support vector machine classifier resulted in reasonable accuracy with a recall of 0.76 and precision 0.78 and allowed us to extend our thematic codes to the entire data set. Conclusions Modeling health-related communication through high throughput methods can enable the identification of specific content related to sustainable chronic disease management, which facilitates targeted health promotion.
Perspective and Evolution of Gesture Recognition for Sign Language: A Review

This review analyses the different gesture recognition systems through a timeline, showing the different types of technology, and specifying which are the most important features and their achieved recognition rates. At the end of the review, Leap Motion sensor possibilities are described in detail, in order to consider its application on the field of sign language. This device has many positive characteristics that make it a good option for sign language. One of the most important conclusions is the ability of the Leap Motion sensor to provide 3D information from the hands for due identification.
Enhanced Expression of Catalase in Mitochondria Modulates NF-κB–Dependent Lung Inflammation through Alteration of Metabolic Activity in Macrophages

Key Points Mitochondrial catalase regulates inflammation by altering cellular metabolism. NAD(H) levels modulate NF-κB activation in macrophages. NF-κB is a reduction-oxidation–sensitive transcription factor that plays a key role in regulating the immune response. In these studies, we intended to investigate the role of mitochondrial-derived reactive oxygen species in regulating NF-κB activation by studying transgenic mice that overexpress mitochondrial-targeted human catalase (mCAT). We treated wild-type (WT) and mCAT mice with intratracheal instillation of Escherichia coli LPS and found that mCAT mice had exaggerated NF-κB activation in the lungs, increased neutrophilic alveolitis, and greater lung inflammation/injury compared with WT mice. Additional studies using bone marrow chimeras revealed that this hyperinflammatory phenotype was mediated by immune/inflammatory cells. Mechanistic studies using bone marrow–derived macrophages (BMDMs) showed that LPS treatment induced a sustained increase in NF-κB activation and expression of NF-κB–dependent inflammatory mediators in mCAT BMDMs compared with WT BMDMs. Further investigations showed that cytoplasmic, but not mitochondrial, hydrogen peroxide levels were reduced in LPS-treated mCAT BMDMs. However, mCAT macrophages exhibited increased glycolytic and oxidative metabolism, coupled with increased ATP production and an increased intracellular NADH/NAD+ ratio compared with BMDMs from WT mice. Treatment of BMDMs with lactate increased the intracellular NADH/NAD+ ratio and upregulated NF-κB activation after LPS treatment, whereas treatment with a potent inhibitor of the mitochondrial pyruvate carrier (UK5099) decreased the NADH/NAD+ ratio and reduced NF-κB activation. Taken together, these findings point to an increased availability of reducing equivalents in the form of NADH as an important mechanism by which metabolic activity modulates inflammatory signaling through the NF-κB pathway.
Modeling Emission Flow Pattern of a Single Cruising Vehicle on Urban Streets with CFD Simulation and Wind Tunnel Validation

Transportation has become one of the primary sources of urban atmospheric pollutants and it causes severe diseases among city residents. This study focuses on assessing the pollutant dispersion pattern using computational fluid dynamics (CFD) numerical simulation, with the effect and results validated by the results from wind tunnel experiments. First, the wind tunnel experiment was carefully designed to preliminarily assess the flow pattern of vehicle emissions. Next, the spatiotemporal distribution of pollutant concentrations around the motor vehicle was modeled using a CFD numerical simulation. The pollutant concentration contours indicated that the diffusion process of carbon monoxide mainly occurred in the range of 0−2 m above the ground. Meanwhile, to verify the correctness of the CFD simulation, pressure distributions of seven selected points that were perpendicular along the midline of the vehicle surface were obtained from both the wind tunnel experiment and the CFD numerical simulation. The Pearson correlation coefficient between the numerical simulation and the wind tunnel measurement was 0.98, indicating a strong positive correlation. Therefore, the distribution trend of all pressure coefficients in the numerical simulation was considered to be consistent with those from the measurements. The findings of this study could shed light on the concentration distribution of platoon-based vehicles and the future application of CFD simulations to estimate the concentration of pollutants along urban street canyons.