Miracle-dz/UniFAR-FTUs huggingface dataset:
Prophylactic Role of Lactobacillus paracasei Exopolysaccharides on Colon Cancer Cells through Apoptosis Not Ferroptosis

Background: Nowadays despite conventional methods in colon cancer treatment, targeting vital molecular pathways and induction of various forms of cell death by safe probiotic components like exopolysaccharides (EPSs) are of great importance and are considered as potential therapeutic agents. This study aimed to investigate the inhibitory effect of the EPS of L. paracasei on different colon cancer cell lines (SW-480, HT-29, and HCT-116). Methods: For this purpose, several cellular and molecular experiments including MTS assay, DAPI staining, Annexin V/PI assay, quantitative real-time PCR (qPCR) and some important ferroptosis-related assays were performed. Results: Based on the findings, L. paracasei EPS can induce apoptosis confirmed by all apoptosis related assays and could not act through ferroptosis pathways. L. paracasei EPS could hinder the Akt1, mTOR, and Jak-1 mRNAs, and induces apoptosis through down-regulation of the antiapoptotic gene (Bcl-2), up-regulation of pro-apoptotic genes (BAX, caspase-3, 8). Conclusion: The exploited EPS of an indigenous probiotic strain with anticancer potential with low/insignificant cytotoxicity to normal cells is proposed for future applications in molecular targeted therapy of colon cancer treatment. Furthermore, in vivo and clinical trials should be performed to evaluate the applicability of this component besides conventional methods to increase the survival rate of colon cancer patients.
The image of Barcelona in Online Travel Reviews during 2017 Catalan independence process

Since the emergence of Online Travel Reviews (OTRs), the collective co-creation of a destination brand image has been more evident than ever before. Stories and emotions expressed in these platforms by experienced tourists can significantly influence other users’ intention to visit. This study explores the extent to which political matters like the incidents that occurred during the last quarter of 2017 related with the Catalan independence process may have a negative impact on the brand image of a destination. This paper has used a specific methodology for content analysis of social media in the field of tourism to study the worst rated OTRs (one-star) of seven different attractions of Barcelona in three social media platforms that are able to geolocate places (TripAdvisor, Google Maps and Facebook) to determine the nature, discourses and the emotions raised. Although most complaints refer to the intrinsic problems of the attractions like high entrance prices and long queues due to tourism overcrowding, results reveal that OTR platforms also include tourists’ personal opinions on such issues as politics and religion. The originality of this research paper is that it delves into users’ co-creation of destination brand image through the analysis of negative OTRs and the emotions expressed in their comments.
The ESCRT-III proteins IST1 and CHMP1B assemble around nucleic acids

ESCRT-III proteins can promote inside-out or outside-in membrane tubulation and fission. In addition, several observations suggest that ESCRT factors may also associate with nucleic acids during development, different stages of the cell cycle, and during retro-transposition of parasitic nucleic acids like LINE1 elements. Two ESCRT-III subunits, IST1 (aka CHMP8) and CHMP1B, can coassemble as an external protein coat around liposomes in vitro and around recycling endosomal tubules in living cells. Here we show that recombinant IST1 and CHMP1B can also copolymerize into double stranded filaments that surround nucleic acids. Electron cryo-microscopy reconstructions of nucleic acid-bound IST1-CHMP1B copolymers revealed that the polynucleotides track along a binding groove formed between filaments of the inner CHMP1B strand. The well-ordered structures also reveal that the C-terminal tails of CHMP1B subunits extrude through the outer IST1 layer to the tube exterior. As a result, the MIT domain binding motifs of both CHMP1B and IST1 are arrayed on the outer surface of the copolymer, where they could bind and recruit MIT domain-containing co-factors, such as the SPASTIN ATPase or the USP8 ubiquitin protease. Our structure raises the possibility that ESCRT-III proteins may form nucleic acid complexes in mammalian cells.
Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae)

Ciguatera fish poisoning is an illness contracted through the ingestion of seafood containing ciguatoxins. It is prevalent in tropical regions worldwide, including in Australia. Ciguatoxins are produced by some species of Gambierdiscus. Therefore, screening of Gambierdiscus species identification through quantitative PCR (qPCR), along with the determination of species toxicity, can be useful in monitoring potential ciguatera risk in these regions. In Australia, the identity, distribution and abundance of ciguatoxin producing Gambierdiscus spp. is largely unknown. In this study we developed a rapid qPCR assay to quantify the presence and abundance of Gambierdiscus lapillus, a likely ciguatoxic species. We assessed the specificity and efficiency of the qPCR assay. The assay was tested on 25 environmental samples from the Heron Island reef in the southern Great Barrier Reef, a ciguatera endemic region, in triplicate to determine the presence and patchiness of these species across samples from Chnoospora sp. , Padina sp. and Sargassum macroalgal hosts. Author’s summary Ciguatera fish poisoning is a human disease contracted by ingesting seafood contaminated with a group of neurotoxins. The group of neurotoxins, named ciguatoxins, are synthesised by species of single celled marine algae from the genus Gambierdiscus. Ciguatera fish poisoning occurs worldwide, particularly in tropical nations. Pacific Island nations are disproportionately impacted, and this impact is predicted to increase as the effects of climate change unfold. Few effective monitoring and mitigation strategies exist for ciguatera fish poisoning, and reporting rates of the disease are estimated to be approximately 20% at best. A global ciguatera strategy was developed by a group of researchers coordinated by UNESCO’s Intergovernmental Oceanographic Commission to characterise the cause and mode of action of ciguatera fish poisoning, as a matter of urgency. In this study, we designed a qPCR assay to detect a species of microalgae, Gambierdiscus lapillus. Gambierdiscus lapillus produces compounds with ciguatoxin-like properties, which may lead to ciguatoxin uptake in fish in the Australian region. This assay was sensitive and able to detect the presence of Gambierdiscus lapillus in a range of environmental samples from the Great Barrier Reef region, Australia.
THE EFFECT OF MARKETING MIX ON CONSUMER SATISFACTION AND ITS IMPACT ON “ROTI MAROS” CONSUMER LOYALTY IN MAROS DISTRICT

Considering the rapid competition of the world economy at this time, so the government must really pay attention to policies, climate change, and renewal of the economy, and participate in making economic policies that have a positive impact in the future. One of the micro businesses as a buffer for the national economy is the food industry which has a very large market demand. The purpose of this study was to determine the effect of marketing mix (price, product, promotion, place) on consumer satisfaction Maros bread, to determine the effect of marketing mix on customer loyalty, to determine the effect of customer satisfaction on customer loyalty, and to determine the effect of marketing mix on loyalty consumers through consumer satisfaction Roti Maros in Maros Regency. The sample used is all consumers who buy Maros bread in Kab. Maros numbering as many as 80 people. Data collection techniques through observation, interviews, documentation and questionnaires. The analysis technique uses validity test, reliability test, descriptive analysis, path analysis, hypothesis testing and sobel test analysis. The results found that the marketing mix had a positive and significant effect on customer satisfaction of Roti Maros. The marketing mix has a positive and significant effect on customer loyalty. Customer satisfaction has a positive and significant effect on customer loyalty Roti Maros. The marketing mix has a positive and significant effect on the customer loyalty of Roti Maros if mediated by customer satisfaction.
Hepatoprotective and nephrotoxic effects of methanol leaf extract of Telfairia occidentalis (Hook f.) in adult female albino rats (Rattus norvegicus).

The leaf extract of the plant Telfairia occidentalis, commonly known as fluted pumpkin is used as a hematinic by post-menstrual and pregnant women and for treatment of many different ailments with little consideration of their toxic potentials. This study assessed the effects of the administration of methanolic leaf extract of Telfairia Occidentalis on serum activity of some enzymes and levels of biochemical indices in female albino rats. Fifteen nulliparous female rats of 12 weeks of age were used for the study. Group A served as untreated control and received only 10 mg/kg distilled water, while groups B and C received 200 and 800 mg/kg body weight of the methanol leaf extract of Telfairia occidentalis respectively for 3 weeks. Results of the study showed that administration of the extract to the treated groups led to significantly (p < 0.05) lower serum activity of alanine aminotransferase on day 14 in group B rats, aspartate aminotransferase on day 7 in group C rats and alkaline phosphatase on day 7 in group B rats. Significantly (p < 0.05) lower serum levels of proteins and cholesterol were recorded for groups B and C rats respectively on day 14. There was significantly (p < 0.05) higher serum urea level in the treated groups on days 14 and 21 and significantly (p < 0.05) higher creatinine level on day 21. It was concluded that administration of methanol extract of T. occidentalis as used in the study might lead to membrane stabilizing effects on hepatocytes, depressed hepatocyte synthetic activity and impaired renal function. Keywords : Telfairia occidentalis . Rattus norvegicus . Ethnomedicine. Nephrotoxicity.
Klasifikasi Kinerja Programmer pada Aktivitas Media Sosial dengan Metode Stochastic Gradient Descent

Kegagalan perusahaan pemula berbasis teknologi (startup) di Indonesia diakibatkan oleh kurang solidnya kinerja tim serta banyaknya kesalahan dalam proses rekrutmen programmer. Kemajuan pesat dalam budaya bermedia sosial dapat dimanfaatkan sebagai salah satu metode untuk memperoleh kandidat programmer terbaik dalam startup. Metode perekrutan yang digunakan dapat berupa melakukan proses klasifikasi konten media sosial kandidat programmer. Klasifikasi tersebut diharapkan dapat menemukan pola kinerja kandidat programmer, dengan hasil baik atau buruk. Metode klasifikasi yang dapat digunakan salah satunya adalah Stochastic Gardient Descent (SGD). Hasil klasifikasi menunjukkan nilai akurasi sebesar 80%, nilai precission 81% dan nilai recall 80%.
A Research on Daily Life Activities and Geriatric Depression Levels of the Elderly in Successful Aging

Technology has gained an important momentum with innovations in the rapid change of today’s world. Data processing (1956), management information system (1965), decision support system (1976), participatory approach (1978), office automation (1980), final user calculation (1981), prototype approach (1982), strategic system planning (1984), information systems that developed as a phenomenological approach (1985) started to be applied in the production sector after 1965 [1]. Today, these technological developments have also affected communication technology. Along with interpersonal communication, communication technology has started to develop in different sectors such as healthcare, home care services, smart home systems, mobile tracking systems, personal security systems, and smartphones. The fact that this sectoral development has reached a significant level with communication technology helps daily life significantly. With distance education, individuals are provided to realize their personal development from their current location. Communication technology has made progress in many areas such as banking transactions, social media, fleet tracking systems, online shopping, video calls, online chat, health, and current information research, hotel reservation, service purchase. These rapid developments have also affected the field of gerontology. Gerontology, a multidisciplinary field with social, cultural, psychological and biological aspects of aging, has been significantly affected by changing technology and innovation. Gerontechnology, which is a multidisciplinary approach that contributes to the improvement of the quality of life of elderly people who try to adapt to their daily life and their environment, and to improve their social, mental and physical conditions, has been an important innovation in this field. Gerontechnology is an important tool in ensuring the active participation of the elderly in life, its harmony with the technological tools, the ergonomic design of technological tools and innovations in terms of the adhesion of the elderly, and providing an important factor in ensuring their physical, social and spiritual support.
Multi-tissue analysis reveals short tandem repeats as ubiquitous regulators of gene expression and complex traits

Short tandem repeats (STRs) have been implicated in a variety of complex traits in humans. However, genome-wide studies of the effects of STRs on gene expression thus far have had limited power to detect associations and provide insights into putative mechanisms. Here, we leverage whole genome sequencing and expression data for 17 tissues from the Genotype-Tissue Expression Project (GTEx) to identify STRs for which repeat number is associated with expression of nearby genes (eSTRs). Our analysis reveals more than 28,000 eSTRs. We employ fine-mapping to quantify the probability that each eSTR is causal and characterize a group of the top 1,400 fine-mapped eSTRs. We identify hundreds of eSTRs linked with published GWAS signals and implicate specific eSTRs in complex traits including height and schizophrenia, inflammatory bowel disease, and intelligence. Overall, our results support the hypothesis that eSTRs contribute to a range of human phenotypes and will serve as a valuable resource for future studies of complex traits.
Analysis of Pan-Tompkins Algorithm Performance with Noisy ECG Signals

The Pan-Tompkins Algorithm is the most widely used QRS complex detector for the monitoring of many cardiac diseases including in arrhythmia detection. This method could provide good detection performance with high-quality clinical ECG signal data. However, the numerous types of noise and artefacts that exist in an ECG signal will produce low-quality ECG signal data. Because of this, the performance of Pan-Tompkins-based QRS detection methods using low-quality ECG signals should be further investigated. In this paper, the performance of the Pan-Tompkins algorithm was analysed in extracting the QRS complex from standard ECG data that includes noise-stressed ECG signals. The algorithm’s QRS detection reliability was tested using MIT-BIH Noise Stress Test data and MIT-BIH Arrhythmia data. The performance of the algorithm was then analysed and presented. This paper shows the capability of the Pan-Tompkins algorithms in handling noisy ECG signals.
A familial Alzheimer’s disease-like mutation in the zebrafish presenilin 1 gene affects brain energy production

To prevent or ameliorate Alzheimer’s disease (AD) we must understand its molecular basis. AD develops over decades but detailed molecular analysis of AD brains is limited to postmortem tissue where the stresses initiating the disease may be obscured by compensatory responses and neurodegenerative processes. Rare, dominant mutations in a small number of genes, but particularly the gene PRESENILIN 1 (PSEN1), drive early onset of familial AD (EOfAD). Numerous transgenic models of AD have been constructed in mouse and other organisms, but transcriptomic analysis of these models has raised serious doubts regarding their representation of the disease state. Since we lack clarity regarding the molecular mechanism(s) underlying AD, we posit that the most valid approach is to model the human EOfAD genetic state as closely as possible. Therefore, we sought to analyse brains from zebrafish heterozygous for a single, EOfAD-like mutation in their PSEN1-orthologous gene, psen1. We previously introduced an EOfAD-like mutation (Q96_K97del) into the endogenous psen1 gene of zebrafish. Here, we analysed transcriptomes of young adult (6-month-old) entire brains from a family of heterozygous mutant and wild type sibling fish. Gene ontology (GO) analysis revealed effects on mitochondria, particularly ATP synthesis, and on ATP-dependent processes including vacuolar acidification.
Towards FoT (Fog-of-Things) enabled Architecture in Governance: Transforming e-Governance to Smart Governance

Cloud computing-based e-Governance is successfully working in many cities nowadays. IoT also plays a vital role in gathering data for various services provided through e-Governance. Various devices are connected to gather and exchange data for different applications by using IoT. IoT has problems like more latency time, security issues, need for high-speed networks for performing computation using cloud computing. These challenges are in the higher end if IoT & cloud are used for e-Governance services. In this paper, the author proposes an FoT (Fog of Things) based architecture for e-Governance as a solution to the above problems. Fog is a type of edge computing in which all the computing is done at a data collection edge/source. Fog will enhance communication between IoT devices and Cloud Computing. We can extend Cloud-of Things to Fog of Things enables the government to move towards smart Governance.
Prisoners of Care: The Experience of Loneliness among Caregivers of Individuals with Alzheimer’s Disease

: Adjusting to an Alzheimer’s disease diagnosis is a complex process. Caregivers tend to experience feelings of loneliness and isolation, which in turn may have adverse effects on their well-being. Although the detrimental effects of caregiving on psychological and physical health are well documented, more research is needed to give a comprehensive portrait of the caregiving experience, especially in the areas of aloneness and social isolation. Loneliness remains a neglected aspect of the caregiving experience. Results from this study offer important insights into the caregiving experience for psychologists and other healthcare professionals.
Determinants of International Financial Integration in ASEAN-5 Countries: Recent Findings Based on Panel GMM Approach

Globalization in the financial and trade sectors has increased capital flows for numerous countries. This study aims to analyze the determinants of international financial integration in ASEAN-5 (Indonesia, Malaysia, Philippines, Singapore and Thailand) from 2008 to 2017. The value for International Financial Integration in this study was calculated using equity-based measurements. This study uses the Generalized Method of Moment (GMM) based on dynamic panel data. The results of this study indicate that all variables significantly affect international financial integration in ASEAN-5, except for domestic credit and exchange rate volatility. Market capitalization, trade openness and GDP per capita have positive relationships to international financial integration, while the inflation variable has a negative relationship to international financial integration.
CHARACTERIZATION OF PROTEIN SOURCES AND THEIR EFFECTS ON BROILER PERFORMANCE, DIGESTIVE TRACT MORPHOLOGY AND CAECAL FERMENTATION METABOLITES

iii ACKNOWLEDGMENTS . iv DEDICATION . v TABLE OF CONTENTS . vi LIST OF TABLES . xi LIST OF FIGURES . xiv LIST OF ABBREVIATIONS . xvi 1.0 INTRODUCTION . 1 2.0 LITERATURE REVIEW . 4 2.1 Factors affecting protein quality in poultry diets. 4 2.1.1 Anti nutritive agents . 4 2.1.1. 1 Glucosinolates . 4 2.1.1.2 Sinapine and tannins. 5 2.1.1.3 Protease inhibitors . 5 2.1.1.4 Phytate . 6 2.1.2 Effects of processing . 8 2.2 Methods for evaluating protein quality . 10 2.2.1 In vivo methods . 10 2.2.2 In vitro methods . 13 2.2.2.1 Chemical methods . 13 2.2.2.2 pH-Stat/Drop method . 15
Factors Associated with Poor Quality of Life in a Canadian Cohort of Patients with Inflammatory Bowel Disease: A Cross-sectional Study

Abstract Background Health-related quality of life (QoL) is often adversely affected in patients with inflammatory bowel disease (IBD). We aimed to identify factors associated with poor QoL among Canadian patients with IBD in clinical remission. Methods We enrolled patients at a single academic tertiary care center with inactive IBD. All eligible patients completed a series of questionnaires that included questions on demographics, disease activity, anxiety, depression and the presence of irritable bowel syndrome (IBS) symptoms. Stool sample for fecal calprotectin (FC) was also collected to assess for subclinical inflammation. The primary outcome measure was QoL assessed by the short inflammatory bowel disease questionnaire (SIBDQ), with planned subgroup comparisons for fatigue, anxiety, depression and IBS symptoms. Results Ninety-three patients were eligible for inclusion in this study. The median SIBDQ scores were lower in patients with anxiety (P < 0.001), depression (P = 0.004), IBS symptoms (P < 0.001) and fatigue (P = 0.018). Elevated FC in patients in clinical remission did not impact QoL. These findings were consistent on multivariate linear regression. Conclusions Anxiety, depression, fatigue and IBS symptoms are all independently associated with lower QoL in patients with inactive IBD. Clinicians are encouraged to screen for these important factors as they may detrimentally impact QoL in IBD patients even in clinical remission.
Factors Influencing Maternal Decision-Making on Infant Feeding Practices

The decision to formula feed or breastfeed a child typically begins with an established prenatal intention. This chapter will examine the multiple dimensions influencing maternal decision-making in regards to the feeding practices of infants including 1) individual maternal characteristics, 2) organizational factors, 3) hospital/provider recommendations, and 4) systematic/policy factors. The chapter will also examine the impact of infant feeding practices on early infant and childhood health outcomes. Research has demonstrated the benefits of breastfeeding on infants and early childhood which includes but is not limited to protection against common illnesses and infections, improved IQ , and even increased school attendance. Moreover, the World Health Assembly global nutrition objectives focus on encouraging breastfeeding support across all sectors in addition to implementing tailored community-based approaches, limiting the excessive marketing of infant formula, and enforcing supportive breastfeeding legislation. The aim of this chapter is to provide an overview of the dynamic interplay between individual, interpersonal, community, and societal factors, such as policies that impact breastfeeding rates and more specifically the health of infants.