Miracle-dz/UniFAR-FTUs huggingface dataset:
Relationship Distress at Home and Burnout Among 254 Pediatric Intensive Care Nurses*

OBJECTIVES: To assess the prevalence of relationship distress and burnout among PICU nurses. DESIGN: Cross-sectional, web-based survey. SETTING: Pediatric intensive care nursing practices in the United States. Subjects: Pediatric intensive care nurses. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: A total of 254 pediatric intensive care nurses in the United States completed the survey consisting of demographic data, practice, and personal characteristics, Revised Dyadic Adjustment Scale, and modified Maslach Burnout Inventory. Relationship distress in consensus was noted in 45.6% of participants, and 26.3% reported distress in relationship satisfaction. Moderate to high burnout was reported by 65% nurses in the emotional exhaustion domain, 43% in depersonalization, and 27% of nurses reported low personal accomplishment. A significant difference in relationship satisfaction was found among nurses identified in different domains of burnout, showing that nurses who scored higher in depersonalization also reported higher distress in relationship satisfaction (p = 0.045). Interestingly, nurses who reported high personal accomplishment (thereby less burnout) reported higher distress in relationship consensus (p = 0.015). The difference in the satisfaction subscale between different age groups was significant, suggesting distress in satisfaction among nurses over the age of 40 (p = 0.004). Comparison of nurses actively involved in marriage counseling with those not actively involved in marriage counseling demonstrated a significant difference in relationship consensus (p = 0.046; odds ratio = 2.46; 95% CI, 0.99–6.06) and satisfaction (p = 0.004; odds ratio = 3.26; 95% CI, 1.42–7.47), suggesting an association between higher relationship distress and counseling. CONCLUSIONS: This study reflects the prevalence of relationship distress and its association with burnout and other practice and personal factors among PICU nurses. Nurses with high depersonalization experienced significantly higher distress in relationship satisfaction, and nurses who reported high personal satisfaction had significantly higher distress in relationship consensus.
The Efficacy of a Home-Based, Self-Administered Hand Exercise Program for Patients With Systemic Sclerosis

Background For patients with systemic sclerosis (SSc), hand involvement is an underrated clinical manifestation. Therefore, the aim of this study was to investigate the efficacy of a hand exercise program and to demonstrate its effect on hand function, quality of life, anxiety, and depression in patients with SSc. Methods This study was designed as a single blind, randomized controlled comparative study. Sixty-two female patients with SSc were randomized into an exercise group (n = 32) or a control group (n = 30). After some were lost to follow-up, 25 patients were analyzed in each group. In the exercise group, the 8-week intervention consisted of isometric hand exercises and self-administered stretching repeated 10 times/2 sets per day. All patients were assessed using the Hand Mobility in Scleroderma (HAMIS) test, the Duruoz Hand Index (DHI), grip strength, the 36-item short form, Health Assessment Questionnaire–Disability Index (HAQ-DI), Beck Anxiety Inventory (BAI), and the Beck Depression Inventory (BDI) at baseline and then again 4 and 8 weeks later. Within-group comparisons over time were analyzed using the Friedman test. Post hoc analysis was performed using the Wilcoxon signed rank test. A multiple linear regression analysis was used to define the impact of exercise on clinical status. Results Of the 50 total patients, the median age and the median body mass index were 55.5 years and 25.9 kg/m2. The median disease duration was 10.0 years. Thirty-four patients (68.0%) were diffuse cutaneous systemic sclerosis (dcSSC), whereas 16 (32.0%) were limited cutaneous systemic sclerosis (lcSSc). The primary outcome of handgrip strength, as well as the HAMIS, DHI, HAQ-DI, and BDI, significantly improved over time (p < 0.001, p < 0.001, p < 0.001, p < 0.001, and p = 0.005, respectively). The between-group comparison indicated significant improvement in DHI, handgrip strength, HAQ-DI, BAI, and BDI in the exercise group (p = 0.02, p = 0.013, p < 0.001, p = 0.015, and p = 0.036, respectively). In the multiple linear regression analysis, exercise was found to be the most efficient factor affecting the improvement in HAMIS, DHI, HAQ-DI, and grip strength. Conclusions The 8-week intervention composed of isometric hand exercises and self-administered stretching provided a significant improvement in handgrip strength, general health, quality of life, and psychological status for patients with SSc.
Intra-articular formulation of colchicine loaded nanoemulsion systems for enhanced locoregional drug delivery: in vitro characterization, 99mTc coupling and in vivo biodistribution studies

Abstract Colchicine (Col) is a drug used mainly for prevention and treatment of acute gouty arthritis. Unfortunately, colchicine has a narrow therapeutic index, with no obvious differentiation between toxic and nontoxic doses, resulting in a great deal of doubt and a disappointing outcome. To surmount such limitation, colchicine nanoemulsion systems (ColNE) were developed using water titration technique. The pseudoternary phase diagrams of surfactant (Span 20 or Span 60 or Tween 80), cosurfactant (ethanol) and oil (IPM) were constructed. The developed ColNE systems were characterized for particle size (PS), polydispersity index (PDI), zeta potential (ZP) and entrapment efficiency (EE %). ColNE-5 was selected as optimized system with PS = 103.34 ± 5.44 nm, ZP = 34.23 ± 0.94 mV, PDI = 0.26 ± 0.01% and EE % = 75.65 ± 0.34%. To track ColNE-5 in vivo, technetium 99 m (99mTc) was incorporated into this system via coupling with colchicine. 99mTc-ColNE-5 and 99mTc-Col solution (99mTc-ColS) were injected intra-articularly (IA) into the inflamed knee joint of Swiss albino mice joints stimulated by MSU crystals then the biodistribution pattern was studied. The findings revealed that IA injection of 99mTc-ColNE-5 significantly enhanced retention and the pharmacodynamic effects of Col compared to 99mTc-ColS. Herein, we concluded that nanoemulsion (NE) could be used as an IA injectable delivery vehicle to improve retention and localization of Col inside the inflamed joint.
Acupuncture therapy and cognitive dysfunction in patients with type 2 diabetes

Abstract Background: With the aging of society, the incidence of type 2 diabetes (T2DM) is increasing every year, and there is a clear correlation between T2DM and cognitive dysfunction. Acupuncture therapy has been widely used in the treatment of T2DM, but there is no systematic review on the treatment of T2DM associated with cognitive impairment. Therefore, this study aimed to conduct a meta-analysis of acupuncture in the treatment of T2DM with cognitive impairment to clarify its efficacy. Methods: A structured and systematic literature search will be conducted in the following databases up to April 26, 2021: PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science (WOS), China National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (CBM), Chinese Scientific and Journal Database (VIP), and Wan Fang database (Wanfang). We will use the Review Manager 5.4 software provided by the Cochrane Collaborative Network for statistical analysis. We then assessed the quality and risk of the included studies and observed the outcome measures. Results: This meta-analysis further determined the beneficial effects of acupuncture on T2DM with cognitive impairment. Conclusion: The purpose of this meta-analysis was to explore the effect of acupuncture on patients T2DM with and cognitive impairment patients, and provide more options for clinicians and patients to treat T2DM with cognitive impairment. Ethics and dissemination: This systematics review will evaluate the efficacy and safety of acupuncture in the treatment of T2DM with cognitive impairment. Since all the data included were published, the systematic review did not require ethical approval. Registration number: CRD42021245681.
Bis(pyrazolato)-Based Metal–Organic Frameworks Fabricated with 4,4′-Bis((3,5-dimethyl-1H-pyrazol-4-yl)methyl)biphenyl and Late Transition Metals

The reaction between the flexible, bis(pyrazolato)-based ligand 4,4′-bis((3,5-dimethyl-1H-pyrazol-4-yl)methyl)biphenyl (H2DMPMB) and late transition metal ions allowed the isolation of the two series of thermally stable metal–organic frameworks [M(DMPMB)] (M = Co, Zn, Cd) and [M2(DMPMB)] (M = Cu, Ag). X-ray powder diffraction (XRPD) structure determinations demonstrated that the anti conformation adopted by the ligand, juxtaposed to its exo-tetradentate coordination mode, induces the formation of a 3-D architecture with nonpervious 1-D channels in the three isomorphous species [M(DMPMB)] and of corrugated 2-D layers in [Cu2(DMPMB)]. Coupling thermogravimetric analyses and variable temperature XRPD highlighted that the five materials are thermally stable at least up to 300 °C, both under nitrogen and in air, the Cd(II) derivative decomposing only at the remarkably high temperature of 500 °C. No thermal event could be detected; all the materials preserve their structural motifs up to decomposition. The adso.
Porcine OCT4 Reporter System can Monitor Species-Specic Pluripotency During Somatic Cell Reprogramming

l Background: The present study examined the activity and function of pig OCT4 enhancer in porcine reprogramming cells. Dual �uorescent protein reporter systems controlled by the upstream regulatory region of OCT4, which is one of the master regulators for pluripotency, are widely used in studies of the mechanism of pluripotency. We analyzed how this reporter system functions in FGF-or LIF-dependent reprogrammed porcine pluripotent stem cells using the previously established porcine-specic reporter system. l Results: Porcine embryonic �broblasts were coinfected with the pOCT4-∆ PE-eGFP (DE-GFP) and pOCT4-∆ DE-DsRed2 (PE-RFP) vectors, and GFP and RFP expression was veri�ed during a DOX-dependent reprogramming process. We demonstrated that the porcine OCT4 distal enhancer and proximal enhancer were activated in different expression patterns simultaneously as the changes in the expression of pluripotent marker genes during the establishment of porcine-induced pluripotent stem cells (iPSCs). l Conclusions: Porcine OCT4 upstream region-derived dual �uorescent protein reporter systems serve as live naïve/primed pluripotency indicators for porcine induced pluripotent cell establishment. This work demonstrates the applicability of the porcine OCT4 upstream region-derived dual �uorescence reporter system, which may be applied to investigations of species-specic pluripotency in porcine-origin cells. These reporter systems may be useful tools for studies of porcine-specic pluripotency, early embryo development and embryonic stem cells.
Integrative review on the incidence of HIV infection and its socio-spatial determinants.

OBJECTIVES to identify the socio-spatial determinants associated with the incidence of infection by the human immunodeficiency virus (HIV) described in national and international literature. METHODS integrative literature review that included original articles indexed in the LILACS, PUBMED, CINAHL, and Scopus databases, from 2015 to 2019. The synthesis of the articles was done in a descriptive manner in two categories according to the type of approach. RESULTS 13 articles were selected, with a predominance of ecological studies. Regions with deficient access to health services, high population density, higher rates of poverty, intense migratory flow, border areas, as well as precarious socioeconomic conditions, and risk behaviors are associated with higher rates of infection. FINAL CONSIDERATIONS the influence of regional inequalities on the incidence of HIV infection is revealed, confirming the relevance of spatial analysis techniques for mapping the distribution of diseases and identifying risk areas.
Dysregulation of PI3K and hippo signaling pathways synergistically induces chronic pancreatitis via Ctgf upregulation.

The role of PI3K and Hippo signaling in chronic pancreatitis (CP) pathogenesis is unclear. Therefore, we assessed the involvement of these pathways in CP by examining the PI3K and Hippo signaling components PTEN and SAV1, respectively. We observed significant decreases in pancreatic PTEN and SAV1 levels in 2 murine CP models: repeated caerulein injection and pancreatic ductal ligation. Additionally, pancreas-specific deletion of Pten and Sav1 (DKO) induced CP in mice. Pancreatic connective tissue growth factor (CTGF) was markedly upregulated in both CP models and DKO mice, and pancreatic CCAAT/enhancer-binding protein alpha (CEBPA) expression was downregulated in the CP models. Interestingly, in pancreatic acinar cells (PACs), CEBPA knockdown reduced PTEN and SAV1 and increased CTGF levels in vitro. Furthermore, CEBPA knockdown in PACs induced acinar-to-ductal metaplasia and activation of cocultured macrophages and pancreatic stellate cells. These results were mitigated by CTGF inhibition. CP in DKO mice was also ameliorated by Ctgf gene deletion, and caerulein-induced CP was alleviated by antibody-mediated CTGF neutralization. Finally, we observed significantly decreased PTEN, SAV1, and CEBPA and increased CTGF levels in human CP tissues compared to nonpancreatitis tissues. Taken together, our results indicate that dysregulation of PI3K and Hippo signaling induces CP via CTGF upregulation.
Age Differences in Open-Mindedness: From 18 to 87-Years of Age

ABSTRACT Background As health care improves and more people work into later age, it is important to understand what impacts open-mindedness has on decision-making. This paper examined the role of aging on open-mindedness. Methods Open-mindedness was measured across 12 studies before data amalgamation. The Actively Open-minded Thinking (AOT) scale and Actively Open-minded Thinking about Evidence (AOT-e) scale measured open-mindedness in this sample (n = 9010) of participants between 18 and 87-years of age. Results Summary AOT positively correlated with AOT-e (r = 0.27). For two subfactors derived from factor analysis based on the AOT, scores for both subfactors positively correlated with AOT-e (subfactor-1: r = 0.17/subfactor-2: r = 0.31) but negatively correlated with age (subfactor-1: r = −0.01/subfactor-2: r = −0.16). Age negatively correlated with both AOT (r = −0.11) and AOT-e (r = −0.13). Regressions revealed that open-mindedness decreased with aging. Age marginally predicted the change in open-mindedness, and sex differences were not a predictor. Conclusion It is proposed that the observed differences are the result of a reluctance to change long-established values and ideas at the cognitive level and cortical changes that occur with aging. In an aging population where more adults work into later age, the decrease in open-mindedness could influence many areas of judgments of decision-making. Importantly, this demonstrates that open-mindedness varies across lifespan.
Unravelling the mechanisms controlling heme supply and demand

Significance Heme is essential for the survival of virtually all living systems and is involved in many fundamental biological processes. It is also implicated as a signaling/regulatory molecule and must be mobilized in response to cellular demands. This presents a complex logistical problem: heme cannot simply diffuse around cells because it is both insoluble and cytotoxic. We show that the cell exhibits exquisite control over release of heme by limiting its availability to one molecule or less within cellular compartments. We suggest an exchange mechanism between protein partners to control supply and demand. Such a mechanism would provide an in-built buffering capacity for heme, enable cells to hoard supplies of heme, and protect the cell against the undesirable effects of heme. In addition to heme’s role as the prosthetic group buried inside many different proteins that are ubiquitous in biology, there is new evidence that heme has substantive roles in cellular signaling and regulation. This means that heme must be available in locations distant from its place of synthesis (mitochondria) in response to transient cellular demands. A longstanding question has been to establish the mechanisms that control the supply and demand for cellular heme. By fusing a monomeric heme-binding peroxidase (ascorbate peroxidase, mAPX) to a monomeric form of green-fluorescent protein (mEGFP), we have developed a heme sensor (mAPXmEGFP) that can respond to heme availability. By means of fluorescence lifetime imaging, this heme sensor can be used to quantify heme concentrations; values of the mean fluorescence lifetime (τMean) for mAPX-mEGFP are shown to be responsive to changes in free (unbound) heme concentration in cells. The results demonstrate that concentrations are typically limited to one molecule or less within cellular compartments. These miniscule amounts of free heme are consistent with a system that sequesters the heme and is able to buffer changes in heme availability while retaining the capability to mobilize heme when and where it is needed. We propose that this exchangeable supply of heme can operate using mechanisms for heme transfer that are analogous to classical ligand-exchange mechanisms. This exquisite control, in which heme is made available for transfer one molecule at a time, protects the cell against the toxic effect of excess heme and offers a simple mechanism for heme-dependent regulation in single-molecule steps.
Zinc-Biofortified Wheat Intake and Zinc Status Biomarkers in Men: Randomized Controlled Trial.

BACKGROUND Biofortification is a novel method for improving the nutritional value of grains. Wheat is widely consumed worldwide. Thus, wheat zinc biofortification may improve the zinc status of populations. OBJECTIVES We determined the effect of consuming zinc-biofortified wheat on plasma zinc concentrations and biomarkers of zinc-dependent functions in a controlled feeding study. METHODS Thirty-six healthy adult men, aged 18 to 51 y, participated in a 10-wk zinc-controlled feeding trial. After a 2-wk run-in period [metabolic period (MP) 1] (9.3 mg zinc/d and 2.1 g total phytate/d) to standardize zinc status, the participants consumed bread made from zinc-biofortified wheat (10.9 mg zinc/d) with no additional phytate (0.6 g/d total phytate) for 6 wk (MP2). During the final 2 wk (MP3), half of the men took a 25-mg zinc supplement daily to determine if the supplement further altered zinc status biomarkers. Repeated-measures linear regression methods were used to compare plasma zinc concentrations, fatty acid desaturase (FADS) activities, glutathione (GSH) concentrations, and DNA strand breaks assessed at enrollment and the end of each metabolic period. RESULTS Plasma zinc concentrations did not change throughout the study. From the end of MP1 to the end of MP2, the conversion of linoleic acid to γ-linolenic acid (FADS2 activity) increased from 0.020 to 0.025 (P = 0.02), and the conversion of dihomo-γ-linolenic acid to arachidonic acid (FADS1 activity) decreased from 6.37 to 5.53 (P = 0.01). GSH concentrations and DNA strand breaks did not change. Zinc supplementation (25 mg/d) in MP3 did not alter any of the endpoints. CONCLUSIONS In healthy adult men, a 1.6-mg/d increase in dietary zinc from biofortified wheat modified FADS2 and FADS1 activities without changing DNA damage, plasma zinc, or GSH concentrations, demonstrating that FADS activities are more sensitive to small changes in zinc consumed with a meal. This trial was registered at clinicaltrials.gov as NCT03451214.
Modeling, learning, perception, and control methods for deformable object manipulation

Enabling robots to handle deformable objects requires careful integration of data-driven and analytic approaches. Perceiving and handling deformable objects is an integral part of everyday life for humans. Automating tasks such as food handling, garment sorting, or assistive dressing requires open problems of modeling, perceiving, planning, and control to be solved. Recent advances in data-driven approaches, together with classical control and planning, can provide viable solutions to these open challenges. In addition, with the development of better simulation environments, we can generate and study scenarios that allow for benchmarking of various approaches and gain better understanding of what theoretical developments need to be made and how practical systems can be implemented and evaluated to provide flexible, scalable, and robust solutions. To this end, we survey more than 100 relevant studies in this area and use it as the basis to discuss open problems. We adopt a learning perspective to unify the discussion over analytical and data-driven approaches, addressing how to use and integrate model priors and task data in perceiving and manipulating a variety of deformable objects.
Viscid–inviscid interactions of pairwise bubbles in a turbulent channel flow and their implications for bubble clustering

Abstract A series of experiments on turbulent bubbly channel flows observed bubble clusters near the wall which can change large-scale flow structures. To gain insights into clustering mechanisms, we study the interaction of a pair of spherical bubbles rising in a vertical channel through combined experiments and modelling. Experimental imaging identifies that pairwise bubbles of 1.0 mm diameter take two preferential configurations depending on their mutual distance: side-by-side positions for a short distance ($S<5$) and nearly in-line, oblique positions for a long distance ($S>5$), where $S$ is the mutual distance normalized by the bubble radius. In the model, we formulate the motions of pairwise bubbles rising at $Re=O(100)$, where $Re$ is the Reynolds number defined based on the bubble diameter. Analytical drag and lift, and semi-empirical spatio-temporal stochastic forcing, are employed to represent the mean acceleration and the fluctuation due to turbulent agitation, respectively. The model is validated against the experiment through comparing Lagrangian statistics of the bubbles. Simulations using this model identify two distinct time scales of the interaction dynamics which elucidate the preferential configurations. For pairs initially in line, the trailing bubble rapidly escapes from the viscous wake of the leading bubble to take the oblique position. Outside of the wake, the trailing bubble travels on a curved path with a slower velocity driven by potential interaction and horizontally approaches the leading bubble to become side by side. Moreover, statistical analysis identifies that the combination of the wake and the agitation can significantly accelerate the side-by-side clustering of in-line pairs. These results indicate positive contributions of liquid viscosity and turbulence to the formation of bubble clusters.
Effects of non-uniform rheology on the motion of bubbles in a yield-stress fluid

Abstract Experimentally, when bubbles rise through yield-stress fluids they create a pathway that is preferentially followed by subsequent bubbles. The formation of such paths is not fully understood rheologically. Here, we instead study the effect of pathways, modelled as a pathway within which the yield stress is destroyed. We study how bubbles rise along such ‘damaged’ channels and how they may be trapped by a combination of capillary effects and the yield stress of the surrounding fluid. We then study the effects of these channels on distant bubbles. We show that the damaged channels attract bubbles that are mobile but lie at a distance well beyond the yielded envelope of the bubble. Angled channels also attract bubbles, that may either move along the channel or migrate outside. Experiments illustrate these behaviours, which are quantified in a series of two-dimensional computations. The study is motivated by interest in bubble release mechanisms in mined tailing ponds.