Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression Levels of miR-590-3p in Hepatocellular Carcinoma and Osteosarcoma Cell Lines and its Downstream Target Genes

Small non-coding RNA (miRNA) sequences of around 18-25 nucleotides can regulate different cellular pathways by acting on tumor suppressors, oncogenes or both. They are mostly tissue-specific and can be up-regulated or down-regulated, depending on the cancer or the tissue in which they are found. hsa-miR-590-3p was found to be involved in several types of cancers. In this study, we used computational analysis to identify potential downstream target genes of hsa-miR-590-3p. We used five databases (TargetScan, miRanda-mirSVR, miRDB, miRTarBase and Diana Tools) to obtain a dataset of potential downstream target genes of hsa-miR-590-3p from each database. We obtained lists of hundreds of genes from these databases. We obtained a shorter list of common genes in all five databases from which we selected fourteen genes with the potential to affect cancer progression and to help further understand the disease pathogenesis, based on previous literature and further in silico analysis using Expression Atlas and The Human Protein Atlas. SOX2, N-cadherin, E-cadherin and FOXA2 were utilized as potential downstream target genes of hsa-miR-590-3p. SNU449 and HepG2, hepatocellular carcinoma cell lines, and U2OS, osteosarcoma cell line, were used to carry out various molecular techniques to further validate our in silico results. mRNA and protein expression levels of these genes were detected using RT-PCR and western blotting respectively. In an attempt to co-localize the hsa-miR-590-3p and its candidate downstream target gene, SOX2, we conducted a miRNA in situ hybridization combined with immunohistochemistry staining using anti-SOX2. The results show that there is an inverse correlation between hsa-miR-590-3p expression and SOX2 protein expression in SNU449 and U2OS. Examining the expression of hsa-miR-590-3p downstream target genes can enhance our understanding of the cancer pathogenesis and how it can be utilized as a therapeutic tool.
Local Differential Privacy and Its Applications: A Comprehensive Survey

With the fast development of Information Technology, a tremendous amount of data have been generated and collected for research and analysis purposes. As an increasing number of users are growing concerned about their personal information, privacy preservation has become an urgent problem to be solved and has attracted significant attention. Local differential privacy (LDP), as a strong privacy tool, has been widely deployed in the real world in recent years. It breaks the shackles of the trusted third party, and allows users to perturb their data locally, thus providing much stronger privacy protection. This survey provides a comprehensive and structured overview of the local differential privacy technology. We summarise and analyze state-of-the-art research in LDP and compare a range of methods in the context of answering a variety of queries and training different machine learning models. We discuss the practical deployment of local differential privacy and explore its application in various domains. Furthermore, we point out several research gaps, and discuss promising future research directions.
Phonological working memory and non-verbal complex working memory as predictors of future English outcomes in young ELLs

Aim of the study: The aim of this longitudinal study was twofold: first, to determine whether the relationship between working memory measures and language performance in young English language learners (ELL) remains constant over the year. The second aim was to determine if performance on working memory tasks predicts future performance on language measures. Methodology: The participants were 27 ELLs between the ages of 5 and 6 years who were in their first year of formal schooling and attended the same mid-low socioeconomic status school in South Africa. The participants were tested three times throughout the year on tasks of working memory and an English assessment battery. Data analysis and results: Mixed effects models and multiple linear regression were used to address the aims of the study. The first aim of the study showed that there are significant correlations between all working memory measures and all language measures in varying strengths across the year. The second research aim further elaborated on this by showing that both phonological working memory and non-verbal complex working memory are implicated in the acquisition of syntax, semantics and pragmatics at different points throughout the year. Implications and originality: Language acquisition in ELLs is not a stand-alone process and working memory measures may be able to predict future language outcomes. This could indicate that working memory measures may be used as an indicator for who may need language intervention, at a time when the ELL only has limited English proficiency and limited English exposure. This research is the first of its kind to originate from Africa, with a sample from low socioeconomic, culturally and linguistically diverse circumstances who are exposed to English consistently for the first time and are tested with working memory tasks with less strong language components.
OPTIMUM REGIONAL IRRIGATION REQUIREMENTS UNDER CHANGING CLIMATE IN BULGARIA

Popova Z, I. Vurlev, M. Ivanova and E. Dimitrov, 2018 optimum regional irrigation requirements under changing climate in Bulgaria, Bulg. J. Agric. Sci., 24 (Suppl. 2):33-42 Net irrigation requirements (NIR, mm) that fully satisfy crop development and yield formation are basic in irrigation systems’ design and management. Bulgarian practice usually adopts the irrigation scheduling developed by Zahariev et al. (1986) that provide information on 31 crops and 97 irrigation regions (IR). Years, having probability of occurrence of an irrigation depth PI=10, PI=25 and PI=50%, were considered. To cope with climate uncertainties and drought aggravation, simulations were performed for past (1950-1980) and present (1951-2004) weather conditions of unified Agro-Climatic AC regions. In former studies the irrigation scheduling simulation WinISAREG model was calibrated for maize using data from long-term experiments carried out in fields of diverse soil, climate and management conditions. Optimal AC regions were defined on the grounds of average reference evapotranspiration totals for July-August relative to the period 1951-2004 EToJul-Aug. Thus, EToJul-Aug served as an indicator of regional NIR and IR unification into AC regions. The impacts of soil properties were characterised by total available soil water TAW, being “small” if TAW=116, “average” if 136<TAW<157 and “large” when 173<TAW<180 mm m-1. NIR were computed by model application to soils of small and large TAW in each AC region and 1951-2004 period. Results indicate that when EToJul-Aug increases from 260 to 330 mm, NIR in “average” demand year (PI=50%) increase from 160 to 310 mm for soils of “small” TAW. Relative to 1951-1980, unified conventional irrigation demands were compared to those simulated. Results showed that the former were mostly in the range of those derived by model simulations. It was concluded that the model took better into account the impact of climate change and different TAW. Maps illustrate findings of the study over country territory in “an average”, “a moderately dry” and “a very dry” season.
“Dude, that Sucks”: Examining Scrum’s Influence on Empathy in Student Teams

ABSTRACT The role of empathy in student team collaborations in technical and professional communication has been understudied. In this mixed methods study, we assess how Scrum affects both student perceptions of empathy and student use of empathetic strategies. We found that students who used Scrum considered themselves to be no more empathetic than students who did not use Scrum. However, a discourse analysis revealed that students who used Scrum deployed significantly more empathetic strategies than students who did not use Scrum.
Social Network Sites Usage and Fear of Missing Out among Female Instagram User

The aim of this investigation was to examine the correlation between social network sites usage and fear of missing out (FOMO) of female instagram user with age ranged from 18 to 27. The responden involved 106 female college student of female instagram user from various university in west, east, and central java, Indonesia. The data collected randomly through an online link Google Form use 2 psychological scale, Student Form Social Media Addiction Scale (SMAS-SF) developed by Sahin and Fear of Missing Out Scale developed by Przybylski. The result show social network sites usage significantly corellate with fear of missing out of female instagram user. Keywords— social network sites, FOMO, Instagram, female user
Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum

Several molds are able to colonize wood and many building products or solid wood causing losses for their valuable uses. Essential oils (EOs) from aromatic plants can be used as an ecofriendly biofungicide against the growth of several molds. EOs from Eucalyptus camaldulensis, Citrus aurantium, and C. sinensis have a broad-spectrum antimicrobial activity. EOs from of E. camaldulensis air-dried aerial parts, C. aurantium leaf and C. sinensis peel, and their combinations (1:1 v/v) were evaluated for their antifungal activity against the growth of four common mold fungi (Aspergillus flavus, A. niger, A. terreus, and Fusarium culmorum). The chemical compositions of the EOs were analyzed with GC/MS. The main compounds in EO from E. camaldulensis were spathulenol (20.84%), eucalyptol (12.01%), and sabinene (9.73%); in C. aurantium were linalyl acetate (42.29%), and linalool (29.76%); and in C. sinensis were D-limonene (73.4%) and γ-terpinene (22.6%). At 50 µL/mL, C. sinensis EO showed the highest fungal mycilial growth inhibition (FMGI) percentage (86.66%) against A. flavus. C. sinensis, E. camaldulensis, and E. camaldulensis/C. sinensis showed FMGI values of 96%, 91.66%, and 75.66% respectively, against A. niger. EOs from C. aurantium and C. sinensis showed potent activity against A. terreus (100% FMGI), while C. aurantium/E. camaldulensis and E. camaldulensis/C. sinensis showed FMGI values of 74.33% and 70.66%, respectively. Potent activity against F. culmorum with 100% was observed as the application of E. camaldulensis and C. sinensis EOs at 50 µL/mL, while E. camaldulensis/C. sinensis (50 µL/mL) showed FMGI value of 65.66%. The results suggest using the EOs and their combinations from E.camaldulensis, C. aurantium, and C. sinensis as a biofungicide against molds. The potent properties of EOs offer the possibility of using them as eco-friendly, safe, and cost-effective antimicrobials for molds that could cause discoloration of the wood packaging or food spoilage.
Degree of conceptual overlap affects eye movements in visual world paradigm

ABSTRACT Several studies employing the visual world paradigm have shown that people will look at visual objects having a semantic relationship with spoken words. However, it remains unclear how eye movements are controlled in the visual world paradigm. The present study examined how listeners guide their eyes in the visual world paradigm by distinguishing the conceptual overlap hypothesis and the uncertainty reduction hypothesis. The uncertainty of the spoken words and the degree of conceptual overlap between visual objects and spoken words were manipulated by varying the spoken words across different hierarchical levels. The result showed that participants looked at the target objects more often when there was greater conceptual overlap between visual objects and spoken words, suggesting that the degree of conceptual overlap between spoken words and visual objects is the major factor that determines the probability of looking at a target object.
Occurrence of Phenolic Endocrine Disruptors in Danube Delta, Romania

In the last decade, the use of chemical compounds with a molecular structure similar to that of BPA has been reported more and more as alternatives to BPA in various industrial products. This comes as a result of banning partial or total use of BPA because of its endocrine disrupting properties. However, bisphenol analogues have been shown to have similar or even greater negative properties than BPA. Thus, particular attention has been given to the risks they have for aquatic systems and human health. In this context, the present study aimed to determine the concentration level at which some of the bisphenol analogues (BPS, BPB, BPE, BPC and BPE), BPA and its major metabolite, 4HAP, are found in surface waters. For this purpose, 11 sampling points were established in the geographical area of the Danube Delta. Among the seven targeted pollutants, only four were detected in the analyzed samples. 4-HAP metabolite was the most abundant compound in the analyzed samples, with concentrations ranging from 3.56 to 30.9 ng/L. BPA concentrations were, in most cases approximately three times lower than those determined for 4-HAP. The next bisphenol analog after 4HAP, in the decreasing order of concentrations, was BPE, for which the concentration level ranged between LOQ and 12.4 ng/L. Lowest concentrations were detected for BPS, with a maximum level of 1.96 ng/L.
Dosing time matters

Circadian precision medicine may supplement genetic precision to improve drug action Physicians diagnose and administer treatment as needed; time of day is rarely considered. Yet, accumulating evidence shows that our molecular clocks orchestrate 24-hour circadian rhythms in vital cardio-metabolic, endocrine, immunologic, and behavioral functions. Time, therefore, adds a potentially important dimension to medicine. Circadian medicine aims to incorporate knowledge of 24-hour biological rhythms to enhance diagnosis and treatment. How is research and technology shaping this endeavor?
The complete mitochondrial genome and phylogenetic analysis of Nerita yoldii (Gastropoda: Neritidae)

Abstract The complete mitochondrial genome of Nerita yoldii has been determined. The whole sequence was 15,719 bp in length and included 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes (12S rRNA and 16S rRNA). The overall base composition is A 29.5%, C 13.8%, G 21.5%, and T 35.2%. In 13 protein-coding genes, the nad2, nad5, and nad6 gene start with ATT, whereas nad4 started with ATA, the other nine genes start with ATG. For the stop codon, COX1, COX3, nad3, and atp8 end with TAG, other nine genes end with TAA. Phylogenetic tree was constructed based on 13 protein-coding genes sequences of Archaeogastropoda species, and the result showed that N. yoldii is most closely related to Clithon retropictus. These mitogenome sequence data will be useful for population genetics and phylogenetic analysis of the family Neritidae.
When the Boss is Far Away and There is Shared Pay: The Effect of Monitoring Distance and Compensation Interdependence on Performance Misreporting

This study investigates how control features influence employees’ perceptions of controls and, subsequently, their reporting behaviors. Specifically, I examine whether implementing a more centralized versus decentralized monitoring control influences employees’ perceptions of whether the control will detect misreported performance. In an experiment that holds the true detection rate constant, I find participants perceive a relatively decentralized control more likely to detect misreporting than a centralized control. Because decisions about monitoring controls and compensation systems are interrelated (O’Donnell 2000) and made contemporaneously (Brenner and Ambos 2013), I also examine whether compensation interdependence influences the effect that control centralization has on misreporting. Prior research documents aggressive reporting behavior increases as compensation becomes more interdependent (Sutter 2009). This suggests that larger increases in perceived detection rates are required to reduce dishonest reporting as compensation interdependency increases. Therefore, for a given level of perceived detection resulting from control centralization, employees are more likely to misreport when compensation is more interdependent than independent. Consistent with this reasoning, I find that a more decentralized control reduces dishonest reporting when compensation interdependence is low. However, when compensation interdependence is high, the effect of control centralization on dishonest reporting is muted. These results suggest that a complex relationship exists between control centralization and compensation interdependence. As such, this study has important implications for academics, control system designers, and compensation system designers.
Peningkatan Hasil Belajar IPA Melalui Model Pembelajaran Group Investigation Berbantuan Media Konkret

Tujuan penelitian ini untuk mengetahui pengaruh model pembelajaran Group Investigation berbantuan media konkret terhadap hasil belajar IPA siswa kelas IV SD di Gugus II Kecamatan Bangli Tahun Pelajaran 2019/2020. Jenis penelitian ini yaitu eksperimen semu dengan desain penelitian Quasi Eksperimen Nonequivalent Posttest Only Control Group Design. Populasi pada penelitian ini adalah seluruh siswa kelas IV di SD Negeri Gugus II Kecamatan Bangli yang berjumlah 180 siswa. Sampel yang digunakan pada penelitian ini yaitu siswa kelas IV SD Negeri 1 Bebalang yang berjumlah 28 orang dan siswa kelas IV SD Negeri 2 Kawan yang berjumlah 30 orang. Pengumpulan data dalam penelitian dilakukan dengan metode tes. Instrumen pengumpulan data berupa tes pilihan ganda. Data Hasil Belajar IPA dianalisis dengan menggunakan teknik analisis statistic deskriptif dan uji prasyarat analisis. Berdasarkan hasil perhitungan nilai rata-rata, diperoleh nilai rata-rata pada kelompok eksperimen sebesar 21 dan kelompok control sebesar 17. Hasil penelitian menunjukkan bahwa thitung sebesar 5,63, sedangkan, ttabel dengan dk 56 pada taraf signifikansi 5% adalah 2,02. Hal ini berarti, thitung lebih besar dibandingkan ttabel (thitung = 5,63 > ttabel = 2.02) sehingga H0 ditolak dan H1 diterima. Dengan demikian, maka dapat disimpulkan bahwa terdapat perbedaan hasil belajar IPA antara kelompok siswa yang dibelajarkan dengan menggunakan model Group Investigation berbantuan media konkret dengan kelompok siswa yang tidak dibelajarkan dengan menggunakan model Group Investigation berbantuan media konkret pada siswa kelas IV SD di Gugus II Kecamatan Bangli.
Within the context of sociological criticism theory, a literary work from the 17th century; Nefhatü’l-Ezhâr

Spanning over a period of six centuries from the 13th century till the 19th century, Classical Turkish Literature takes up a reputed position in Oriental Literature. In the earliest centuries, classical Turkish literature was heavily influenced by Arabic and Persian Literature; however upon completing its foundation, it started to embrace a domestic and national character as of 16th century. With the advent of 16th century, particularly in masnavis as used to narrate lengthy stories, a different path was taken from other Oriental literatures in regards to characters and settings in stories. Stories, then, began to evolve within the borders of Ottoman territory and a wider place was reserved to take notice of witnessed problems. In some of these masnavis it is feasible to come across social reflections on the specific period and certain expressions that would most probably not approved at an age this work was compiled. In that sense one of the salient examples is Nefhatü’l-Ezhâr masnavi written by 17th - century poet Nev’i-zâde Atâyî. In Nefhatü’l-Ezhâr it is detected that defects that the poet witnesses in his society are narrated to his readers in short stories that develop within a plot. In such stories, Atâyî criticizes the kind of people exploiting religion for personal gains and those simple men licking powerful men's shoes for self-interest. In relation to social criticism stealing and injustice of rulers are highlighted-issues by the poet. Further to that, by narrating obscene stories, the poet attempts to unveil a form of corruption that has eroded moral fiber of community. In order to better grasp a literary text and locate the author's messages aimed for the reader, there is need to approach a work from a wide range of perspectives. In our research, stories that are considered to reflect traces from society in the said work of Nev’i-zâde Atâyî will be elaborated within the context of sociological criticism.
An efficient method to compute simplified noise parameters of analog amplifiers using symbolic and evolutionary approach

Symbolic circuit analysis suffers as the symbolic terms increases by many folds with the increase in the size of the circuit. Hence simplification techniques are used for the analysis of the circuits which are larger in size. In this work, multi‐objective evolutionary optimisation algorithms for obtaining simplified symbolic expressions of analog amplifier circuits are presented which are not only very efficient in terms of speed and the amount of circuit reduction but also solve the accuracy problems. Initially, all MOSFETs are replaced with their nullor equivalent model. Then, the formulation of accurate fully‐symbolic noise expressions is carried out by the application of symbolic nodal analysis. The derived exact symbolic expressions are then simplified using an evolutionary approach. This method has been successfully coded in MATLAB. The numerical results obtained before and after simplification are compared with SPICE simulation to demonstrate the efficiency of the proposed methodology.
Addressing Public Mental Health Challenges: A Mixed-Methods Evaluation of Problem-Based Learning

Problem-based learning (PBL) is a pedagogical framework that centers on a problem of interest and structures classroom tasks around teams formulating practical, evidence-based solutions. This approach is particularly well-suited for public health curricula, as it prepares students for a dynamic, interdisciplinary, professional environment. This study explores the piloting of a PBL approach with three student cohorts in a graduate-level public mental health course. Biweekly, interdisciplinary student teams were presented with a public mental health case provided by an expert in the field and asked to develop and present evidence-based solutions through a process grounded in knowledge acquisition, critical thinking, and faculty guidance. A mixed-methods evaluation was completed to examine student perceptions of PBL components and the benefits of PBL and to gather recommendations to enhance this approach. Among 37 students who completed self-administered surveys, more than 70% found case analysis, reviewing additional literature to support case solutions, and discussions with an expert to be the components “Most Helpful” to their learning. Four focus groups with 21 students were conducted to further examine perceived course benefits and suggested changes. Participants appreciated real-world simulations of public health issues and interdisciplinary teams and offered recommendations for future improvements, such as adding a culminating project. Findings support the benefits of a PBL approach in equipping graduate students with the knowledge and skills to successfully pursue professional public health work. Recommendations inform future efforts to implement this approach in other public health curricula as well as for other courses and settings.