Miracle-dz/UniFAR-FTUs huggingface dataset:
Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input

Controlling gain of cortical activity is essential to modulate weights between internal ongoing communication and external sensory drive. Here, we show that serotonergic input has separable suppressive effects on the gain of ongoing and evoked visual activity. We combined optogenetic stimulation of the dorsal raphe nucleus (DRN) with wide-field calcium imaging, extracellular recordings, and iontophoresis of serotonin (5-HT) receptor antagonists in the mouse visual cortex. 5-HT1A receptors promote divisive suppression of spontaneous activity, while 5-HT2A receptors act divisively on visual response gain and largely account for normalization of population responses over a range of visual contrasts in awake and anesthetized states. Thus, 5-HT input provides balanced but distinct suppressive effects on ongoing and evoked activity components across neuronal populations. Imbalanced 5-HT1A/2A activation, either through receptor-specific drug intake, genetically predisposed irregular 5-HT receptor density, or change in sensory bombardment may enhance internal broadcasts and reduce sensory drive and vice versa.
Factorial Structures of Postpartum Bonding Questionnaire (PBQ): A Systematic Review

Background Postpartum Bonding Questionnaire (PBQ) is a remarkable tool to assess impairments in the mother-infant bonding. The purpose of the present study was to review the factorial structure of the PBQ cross different versions. Materials and Methods The search strategy was implemented on databases of Medline (via PubMed), Scopus, Cochran library and Web of Science through electronic databases. The quality assessment was fulfilled by COSMIN checklist. Results: The four factors were identified in original English version of PBQ. Theses consisted of "impaired bounding", "rejection and anger", "anxiety about care of the baby", and "risk of abuse". Three-factor-solution was confirmed by other English versions. In the Italian version of PBQ, three factors emerged including "annoyance and anger toward the infant", "detachment and rejection" and "anxiety about infant care". In the Spanish version of the PBQ, four factors including "impaired bonding", "anxiety about care", "lack of enjoyment and affection for the baby", and "rejection and risk of abuse". Three studies assessed factorial structure of Japanese version of PBQ. In the first Japanese version, four factors with 14 items were emerged including "impaired bonding ", "rejection and anger" and "anxiety about care", and "lack of affection". In second adapted Japanese version, exploratory factor analysis (EFA) identified a three factor solution: "mother's annoyance with anger toward their baby", "lack of affection" and" rejection and fear". The third version identified one-single solution. In the German version, one single factor emerged that explained 31 of the total variance. Conclusion Original English version of PBQ was not confirmed in Japanese, Italian, Spanish and other English versions.
Assessment of Salivary Cortisol and Perceived Stress in Teachers

Background: This study aimed at correlating the levels of salivary cortisol and perceived stress in male teachers, in three periods of the academic year (March, July, December). Methods: The collection of saliva for cortisol measurement was performed at 6:30 a.m., 11:30 a.m., and 5:30 p.m. on the same day that the BPSS-10 (Perceived Stress Scale, Brazilian version) was applied. The total concentration of cortisol was calculated using the area under the curve with respect to ground (AUCg). Results: When comparing the periods of the academic year, the mean stress score obtained in the month of March (16.46 ± 5.36) and December (17.54 ± 6.56) showed a significant difference compared with the mean results obtained in July (10.77 ± 5.64; p <0.01 and p <0.001. The AUCg value showed no significant difference between the periods analyzed (1.145 ± 0.450 µg/dL, 0.916 ± 0.341 µg/dL, and 1.031 ± 0.374 µg/dL, respectively). When analyzing the correlation between BPSS-10 values and total salivary cortisol throughout the school year, no statistical significance was found according to Pearson correlation coefficient (p = 0.554). Conclusions: The results suggest that the teachers, during the vacation period, might not perceive the physiological reactions of stress, so these reactions were not identified by the BPSS-10 used.
“When I Retire, I’ll Move Out of the City”: Mental Well-being of the Elderly in Rural vs. Urban Settings

This study examines the influence of risk factors on mental well-being at older ages focusing on the level of rurality of the living environment. We used cross-sectional, nationally representative survey data for Catalonia (Spain) from 2015 to 2017 to explain the mental well-being of the population aged 65 years and over. Based on a sample of 2621 individuals, we created a score of current mental well-being using the Short Warwick-Edinburgh Mental Well-being Scale (SWEMWBS). Using logistic regression and non-parametric tests, we identified the sociodemographic, health and lifestyle variables which, in combination with the features of the rural and urban settings of the living space, were associated with poor SWEMWBS scores. Our results reveal that adequate social support is linked to expectations of good mental well-being in later life. Poor self-perceived health and ageing limitations are associated with less deterioration of the well-being for the elderly living in rural areas, whereas living in urban areas is linked to a higher risk of suffering from emotional distress attributable to economic difficulties or low educational attainment. Incentivizing older people to live in rural environments could result in greater well-being in the last stages of life; appropriate prospective studies are needed to test this positive outcome.
Internet Hospitals Help Prevent and Control the Epidemic of COVID-19 in China: Multicenter User Profiling Study

Background During the spread of the novel coronavirus disease (COVID-19), internet hospitals in China were engaged with epidemic prevention and control, offering epidemic-related online services and medical support to the public. Objective The aim of this study is to explore the role of internet hospitals during the prevention and control of the COVID-19 outbreak in China. Methods Online epidemic-related consultations from multicenter internet hospitals in China during the COVID-19 epidemic were collected. The counselees were described and classified into seven type groups. Symptoms were recorded and compared with reported patients with COVID-19. Hypochondriacal suspicion and offline visit motivation were detected within each counselees’ group to evaluate the social panic of the epidemic along with the consequent medical-seeking behaviors. The counselees’ motivation and the doctors’ recommendation for an offline visit were compared. Risk factors affecting the counselees’ tendency of hypochondriacal suspicion and offline visit motivation were explored by logistic regression models. The epidemic prevention and control measures based on internet hospitals were listed, and the corresponding effects were discussed. Results A total of 4913 consultations were enrolled for analysis with the median age of the counselees at 28 years (IQR 22-33 years). There were 104 (2.12%) healthy counselees, 147 (2.99%) hypochondriacal counselees, 34 (0.69%) exposed counselees, 853 (17.36%) mildly suspicious counselees, 42 (0.85%) moderately suspicious counselees, 3550 (72.26%) highly suspicious counselees, and 183 (3.72%) severely suspicious counselees. A total of 94.20% (n=4628) of counselees had epidemic-related symptoms with a distribution similar to those of COVID-19. The hypochondriacal suspicion (n=2167, 44.11%) was common. The counselees’ motivation and the doctors’ recommendation for offline visits were inconsistent (P<.001) with a Cohen kappa score of 0.039, indicating improper medical-seeking behaviors. Adult counselees (odds ratio [OR]=1.816, P<.001) with epidemiological exposure (OR 7.568, P<.001), shortness of breath (OR 1.440, P=.001), diarrhea (OR 1.272, P=.04), and unrelated symptoms (OR 1.509, P<.001) were more likely to have hypochondriacal suspicion. Counselees with severe illnesses (OR 2.303, P<.001), fever (OR 1.660, P<.001), epidemiological exposure history (OR 1.440, P=.01), and hypochondriacal suspicion (OR 4.826, P<.001) were more likely to attempt an offline visit. Reattending counselees (OR 0.545, P=.002) were less motivated to go to the offline clinic. Conclusions Internet hospitals can serve different types of epidemic counselees, offer essential medical supports to the public during the COVID-19 outbreak, reduce the social panic, promote social distancing, enhance the public’s ability of self-protection, correct improper medical-seeking behaviors, reduce the chance of nosocomial cross-infection, and facilitate epidemiological screening, thus, playing an important role on preventing and controlling COVID-19.
Effect of lipid on surimi gelation properties of the three major Chinese carps.

BACKGROUND Gel properties are important in determining the quality of surimi. In addition to myofibrillar proteins, lipids play an important role in the formation of surimi gel. Phospholipids (PL) are amphoteric lipids that cannot be removed through rinsing. Paradoxically, PL addition increases or decreases gel strength. This research aimed to investigate the effects of specific lipids on the gelation properties of surimi from three different carp. RESULTS The hardness, chewiness, and gel strength of bighead carp (Aristichthys nobilis: BC) surimi were higher, whereas the total lipid content was lower when compared with grass carp (Ctenopharyngodon idellus: GC) and silver carp (Hypophthalmichthys molitrix: SC) surimi. BC surimi had lower levels of phosphatidylethanolamine (PE), phosphatidylinositols (PI), and phosphatidylcholine (PC) and higher contents of phosphatidylserines (PS) and sphingomyelins (SM). The gelation properties of surimi increased with increasing concentrations of SM and PS. Furthermore, increased levels of SFA and decreased levels of PUFA increased gelation properties. Finally, higher hydrophobic interactions and more disulfide bonds were shown to increase gel network structure stability, resulting in improving gel strength in BC surimi. CONCLUSION The textural characteristics and gel strength of surimi were dependent on PL contents, including total lipid levels and the types of fatty acids which may account for previous conflicting reports on PLs effects on gel strength. This study provides insight into how the texture of surimi can be improved and provides a starting point for further research. This article is protected by copyright. All rights reserved.
[18F]FET-βAG-TOCA: The Design, Evaluation and Clinical Translation of a Fluorinated Octreotide

The success of Lutathera™ ([177Lu]Lu-DOTA-TATE) in the NETTER-1 clinical trial as a peptide receptor radionuclide therapy (PRRT) for somatostatin receptor expressing (SSTR) neuroendocrine tumours (NET) is likely to increase the demand for patient stratification by positron emission tomography (PET). The current gold standard of gallium-68 radiolabelled somatostatin analogues (e.g., [68Ga]Ga-DOTA-TATE) works effectively, but access is constrained by the limited availability and scalability of gallium-68 radiopharmaceutical production. The aim of this review is three-fold: firstly, we discuss the peptide library design, biological evaluation and clinical translation of [18F]fluoroethyltriazole-βAG-TOCA ([18F]FET-βAG-TOCA), our fluorine-18 radiolabelled octreotide; secondly, to exemplify the potential of the 2-[18F]fluoroethylazide prosthetic group and copper-catalysed azide-alkyne cycloaddition (CuAAC) chemistry in accessing good manufacturing practice (GMP) compatible radiopharmaceuticals; thirdly, we aim to illustrate a framework for the translation of similarly radiolabelled peptides, in which in vivo pharmacokinetics drives candidate selection, supported by robust radiochemistry methodology and a route to GMP production. It is hoped that this review will continue to inspire the development and translation of fluorine-18 radiolabelled peptides into clinical studies for the benefit of patients.
Fabrication of PCL Scaffolds by Supercritical CO2 Foaming Based on the Combined Effects of Rheological and Crystallization Properties

Polycaprolactone (PCL) scaffolds have recently been developed via efficient and green supercritical carbon dioxide (scCO2) melt-state foaming. However, previously reported gas-foamed scaffolds sometimes showed insufficient interconnectivity or pore size for tissue engineering. In this study, we have correlated the thermal and rheological properties of PCL scaffolds with their porous morphology by studying four foamed samples with varied molecular weight (MW), and particularly aimed to clarify the required properties for the fabrication of scaffolds with favorable interconnected macropores. DSC and rheological tests indicate that samples show a delayed crystallization and enhanced complex viscosity with the increasing of MW. After foaming, scaffolds (27 kDa in weight-average molecular weight) show a favorable morphology (pore size = 70–180 μm, porosity = 90% and interconnectivity = 96%), where the lowest melt strength favors the generation of interconnected macropore, and the most rapid crystallization provides proper foamability. The scaffolds (27 kDa) also possess the highest Young’s modulus. More importantly, owing to the sufficient room and favorable material transportation provided by highly interconnected macropores, cells onto the optimized scaffolds (27 kDa) perform vigorous proliferation and superior adhesion and ingrowth, indicating its potential for regeneration applications. Furthermore, our findings provide new insights into the morphological control of porous scaffolds fabricated by scCO2 foaming, and are highly relevant to a broader community that is focusing on polymer foaming.
Failure to Down-Regulate miR-154 Expression in Early Postnatal Mouse Lung Epithelium Suppresses Alveologenesis, with Changes in Tgf-β Signaling Similar to those Induced by Exposure to Hyperoxia

Background: Bronchopulmonary dysplasia (BPD) is a lung disease of preterm born infants, characterized by alveolar simplification. MicroRNA (miR) are known to be involved in many biological and pathological processes in the lung. Although a changed expression has been described for several miR in BPD, a causal role remains to be established. Results: Our results showed that the expression level of miR-154 increases during lung development and decreases postnatally. Further, hyperoxia treatment maintains high levels of miR-154 in alveolar type 2 cells (AT2). We hypothesized that the decrease in miR-154 expression in AT2 cells is required for normal alveologenesis. To test this hypothesis, we generated a novel transgenic mouse allowing doxycycline-based miR-154 overexpression. Maintenance of miR-154 expression in the postnatal distal lung epithelium under normoxia conditions is sufficient to reproduce the hypoalveologenesis phenotype triggered by hyperoxia. Using a pull-down assay, we identified Caveolin1 as a key downstream target of miR-154. Caveolin1 protein is downregulated in response to overexpression of miR-154. This is associated with increased phosphorylation of Smad3 and Tgf-ß signaling. We found that AT2 cells overexpressing miR-154 display decreased expression of AT2 markers and increased expression of AT1 markers. Conclusion: Our results suggest that down-regulation of miR-154 in postnatal lung may function as an important physiological switch that permits the induction of the correct alveolar developmental program, while conversely, failure to down-regulate miR-154 suppresses alveolarization, leading to the common clinically observed phenotype of alveolar simplification.
Assessing stress among medical students in Anbar governorate, Iraq: a cross-sectional study

Introduction A high prevalence of stress has been recorded among medical students worldwide. Additionally, high levels of personal distress may have a negative effect on the cognitive functioning and learning abilities of medical students. Methods A cross-sectional study was conducted using medical students in the Al-Anbar governorate; data collection was carried out from February to March 2018. The assessment of stress levels among these students was administered using the Kessler10 Psychological Distress instrument (K10). Results 231 students (77.5%) reported at least some degree of stress, ranging between severe (30.2%), moderate (25.5%) and mild (21.8%). The highest proportion of students with stress (11.5%) was reported by first-year students; this proportion largely fell within the moderate and severe categories. There was a significant statistical association between gender and stress level. Conclusion There is a high level of stress among medical students in Falluja and Anbar Universities; this may require special programmes to identify any predisposing factors.
The obesity paradox: does it exist in the perioperative period?

At least 30% of the surgical population consists of patients who fulfill the definition of obesity, mirroring the obesity epidemic in the general population in the United States. Nearly 74% of the participants in the American College of Surgeons National Surgical Quality Improvement Program (NSQIP) had an abnormally high body mass index (BMI), including 17% of patients younger than 18 years of age. This development is of particular concern as obesity is associated with an increased risk of metabolic syndrome, hypertension, coronary artery disease (CAD), and diabetes mellitus. However, physicians, nutritionists, and the general public struggle with definitions of obesity based only on total body weight proportionate to height and, therefore, BMI. This leads to the question of whether BMI can adequately describe the physical condition of and potential risks in our patients.
Ultrastructural analysis of the submandibular sialoliths: Raman spectroscopy and electron back-scatter studies

ABSTRACT The aim of work was the epidemiological analysis of the occurrence of sialolithiasis of the submandibular gland in adults and the evaluation of the ultrastructure of salivary stones. The study sample consisted of 44 sialoliths. Analysis of the structure and chemical composition of sialoliths was performed using a Scanning Electron Microscope and Raman Spectroscopy. Comparing our results with the literature we can say that the epidemiology of sialolithiasis has not changed significantly over the past 50 years. A wide variety of sialoliths structure was observed. In 75% (33) cases a layered structure of salivary stones was observed, while in 25% (11) – homogeneous structure. The various distribution of organic and inorganic components was observed among all the analyzed sialoliths. Raman spectroscopy allows for preliminary analysis of the sialoliths structure with only a qualitative assessment of their composition, which significantly reduces the research value of this method. The presence of organic and inorganic compounds in the core and inner layers of the salivary glands stones confirms 2 basic theories of the formation of sialoliths: inflammation and deposition of the inorganic component as a result of disruption of saliva flow in the salivary glands.
3D Hierarchical, Nanostructured Chitosan/PLA/HA Scaffolds Doped with TiO2/Au/Pt NPs with Tunable Properties for Guided Bone Tissue Engineering

Bone tissue is the second tissue to be replaced. Annually, over four million surgical treatments are performed. Tissue engineering constitutes an alternative to autologous grafts. Its application requires three-dimensional scaffolds, which mimic human body environment. Bone tissue has a highly organized structure and contains mostly inorganic components. The scaffolds of the latest generation should not only be biocompatible but also promote osteoconduction. Poly (lactic acid) nanofibers are commonly used for this purpose; however, they lack bioactivity and do not provide good cell adhesion. Chitosan is a commonly used biopolymer which positively affects osteoblasts’ behavior. The aim of this article was to prepare novel hybrid 3D scaffolds containing nanohydroxyapatite capable of cell-response stimulation. The matrixes were successfully obtained by PLA electrospinning and microwave-assisted chitosan crosslinking, followed by doping with three types of metallic nanoparticles (Au, Pt, and TiO2). The products and semi-components were characterized over their physicochemical properties, such as chemical structure, crystallinity, and swelling degree. Nanoparticles’ and ready biomaterials’ morphologies were investigated by SEM and TEM methods. Finally, the scaffolds were studied over bioactivity on MG-63 and effect on current-stimulated biomineralization. Obtained results confirmed preparation of tunable biomimicking matrixes which may be used as a promising tool for bone-tissue engineering.
Antimicrobial Activity of Selected Banana Cultivars Against Important Human Pathogens, Including Candida Biofilm

Ten banana (Musa spp.) cultivars were studied for their antimicrobial properties. Three plant parts (corm, pseudostem and leaves) were collected separately and extracted with different solvents, viz., hexane, acetone, ethanol and water. The 50% inhibitory concentration (IC50) was evaluated using a broth microdilution assay. Eight human bacterial and one fungal pathogen were tested. Acetone and ethanol extract(s) often exhibited significant antimicrobial activity, while hexane extracts were less active. Aqueous extracts often showed microbial growth, possibly by endophytes. Leaf extracts were most active, followed by pseudostem, and corm was least active. All the tested banana cultivars were found to contain antimicrobials, as demonstrated by inhibition of selected human pathogens. However, cultivars such as Dole, Saba, Fougamou, Namwah Khom, Pelipita and Mbwazirume showed a broad-spectrum activity, inhibiting all tested pathogens. Other cultivars such as Petit Naine and Kluai Tiparot showed a narrow-spectrum activity, including antibiofilm activity against Candida albicans. Our results support the use of different parts of banana plants in traditional human medicine for infections, including diarrhea and dysentery, and some sexually transmitted diseases, as well as for packaging spoilable materials like food.