Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of glioma-associated antigens for brain tumor immunotherapy

Glioma is a tumor occurring in the brain and one of the most devastating cancers, affecting children and young adults and having a very high morbidity with poor prognosis. The propensity of glioma cells to invade normal brain structures and to resist current treatments makes new therapeutic strategies an urgent need for this disease. Among them, tumor immunotherapy, defined as use of the immune system to fight cancer, is showing very promising results in many malignancies. The knowledge that the rules governing immune responses in other tissues are also valid for the brain has allowed development of immunotherapy for glioma. Among currently investigated glioma immunotherapies, T cell vaccines and adoptive cell therapy are restrained by the availability of tumor-derived antigens specifically expressed on tumor cells but absent, or expressed at very low levels, on normal cells, to avoid collateral damage on healthy parts of the body. Thus, for glioma, an incessant search for tumor-associated antigens has been ongoing, and we contributed to this field by identifying novel glioma-associated peptides with demonstrated presentation at the tumor cell surface, which we will present in the first part of this thesis. These results have been readily translated into clinical application via formulation of a peptide vaccine (IMA950) that is currently being tested with Poly-ICLC in grade IV glioma patients in our institution with promising results. We will present this trial and preliminary immunomonitoring data in the second part of this work. Then, to further extend the use of the IMA950 peptide vaccine to patients with less aggressive disease, who are more prone to develop clinically relevant immune responses, we assessed expression of the IMA950 antigens in a series of glioma patients with grade II and III tumors. This will be developed in the third part of this report. Finally, the discussion will integrate some early results on the effect of radiotherapy and chemotherapy on the immune system of patients, in order to understand how to better combine our vaccine with standard treatments. All in all, we will show that our research resulted in the development of a new peptide vaccine for patients with glioma, which is in clinical trial and can be proposed through all tumor grades.
5-{2-[(2-Hydroxy-5-methylphenyl)(phenyl)methyleneamino]phenyliminomethyl}pyrrole-2-carbaldehyde

The title compound, C26H21N3O2, is an unsymmetrical tetradentate Schiff base ligand. The hydroxy group forms an intramolecular O—H⋯N hydrogen bond with an adjacent N atom. An intermolecular N—H⋯O hydrogen bond creates centrosymmetric dimers in the crystal packing.
Investigating the engagement of New Zealand diagnostic radiographers with research

• This Thesis represents my own work; • The contribution of supervisors and others to this work was consistent with the Unitec Regulations and Policies. • Research for this work has been conducted in accordance with the Unitec Research Ethics Committee Policy and Procedures, and has fulfilled any requirements set for this project by the Unitec Research Ethics Committee. Research Ethics Committee Approval Number: 1354931
3,3-Dichloro-1-(chloromethyl)indolin-2-one

In the title compound, C9H6Cl3NO, the pyrrole ring is almost coplanar with the benzene ring [dihedral angle = 1.90 (9)°], while the Cl—C—N—C torsion angle is 98.78 (17)°. In the crystal, pairs of molecules are interconnected by pairs of Cl⋯Cl interactions [3.564 (5) Å], forming dimers, which are further peripherally connected through intermolecular C—H⋯O=C and π–π interactions [centroid–centroid distances = 4.134 (7), 4.134 (6) and 4.238 (7) Å], forming a two-dimensional network.
4-[(E)-(4-Hydroxybenzylidene)amino]-3-methyl-1H-1,2,4-triazole-5(4H)-thione

The title compound, C10H10N4OS, is nearly planar with the mean planes of the hydroxybenzyl and triazole rings inclined at an angle of only 3.2 (7)°. In the crystal, O—H⋯N hydrogen bonds between the hydroxy group and the triazole ring in concert with weak N—H⋯S intermolecular interactions between the triazole ring and thione group form chains along [-210] enclosing R 2 2(8) graph-set motifs. A weak intramolecular C—H⋯S interaction and intermolecular π–π interactions [centroid–centroid distance = 3.5990 (15) Å] are also observed.
4-Methyl-N-[(E)-4-methyl-1-(4-methylphenylsulfonyl)-1,2-dihydropyridin-2-ylidene]benzenesulfonamide

The reaction of 2-(aminomethyl)pyridine and 4-toluenesulfonyl chloride in CH2Cl2 at pH 8 led to the title compound, C20H20N2O4S2. The aromatic rings are almost perpendicular to each other and the dihedral angles between the aromatic ring planes are 74.33 (9) (central pyridine versus benzene ring of the tosyl group bonded to the imine functionality), 73.77 (6) (pyridine versus benzene ring of the tosyl group bonded to pyridinic N atom) and 79.83 (9)° (benzene rings of tosyl groups). In the crystal structure, intermolecular aromatic π–π stacking interactions [centroid–centroid separation = 3.6274 (14) Å] help to consolidate the packing.
1-[2-(2,4-Dichlorobenzyloxy)-2-(furan-2-yl)ethyl]-1H-benzotriazole

In the title compound, C19H15Cl2N3O2, the benzotriazole ring system is approximately planar [maximum deviation = 0.018 (2) Å] and its mean plane is oriented at dihedral angles of 30.70 (5) and 87.38 (4)°, respectively, to the furan and benzene rings while the dihedral angle between furan and benzene rings is 74.46 (6)°. In the crystal, weak C—H⋯N hydrogen bonds link the molecules into chains along the b axis. π–π stacking interactions between the parallel dichlorobenzene rings of adjacent molecules [centroid–centroid distance = 3.6847 (9) Å] and weak C—H⋯π interactions are also observed.
Tricyclohexyl(3,5-dibromo-2-hydroxybenzoato-κO)tin(IV)

In the title compound, [Sn(C6H11)3(C7H3Br2O3)], the Sn atom is four-coordinate and possesses a distorted Sn(C3O) tetrahedral geometry, with Sn—C bond lengths in the range 2.132 (6)–2.144 (6) Å and with Sn—O = 2.086 (4) Å. The uncoordinated carboxylate O atom forms a weak contact with the Sn atom, with an Sn⋯O separation of 2.962 (2) Å.
(E)-3-(3-Bromophenyl)-1-(4-methylphenyl)prop-2-en-1-one

The title compound, C16H13BrO, was synthesized from the reaction of 3-bromobenzaldehyde and 4-methylacetophenone in the presence of KOH. The molecule adopts an E configuration with respect to the C=C double bond of the propenone unit. The dihedral angle formed by the aromatic rings is 46.91 (14)°. The crystal structure is stabilized by Br⋯Br interactions [3.4549 (11) Å].
2-Chloro-3-(4-methylanilino)naphthalene-1,4-dione

In the title compound, C17H12ClNO2, the naphthoquinone system is essentially planar [maximum deviation = 0.078 (2) Å] and makes a dihedral angle of 52.38 (7)° with the benzene ring. The crystal structure features N—H⋯O interactions.
[(Z)-N,O-Disopropylthiocarbamato-κS](tricyclohexylphosphine-κP)gold(I)

In the title compound, [Au(C7H14NOS)(C18H33P)], the AuI atom is coordinated within an S,P-donor set that defines a slightly distorted linear geometry [S—Au—P = 174.94 (2)°], with the distortion due to a short intramolecular Au⋯O contact [2.908 (2) Å].
1-{(1Z)-1-[3-(2,4-Dichlorophenoxy)propoxy]-1-(2,4-difluorophenyl)prop-1-en-2-yl}-1H-1,2,4-triazole

In the title compound, C20H17Cl2F2N3O2, the triazole ring makes dihedral angles of 28.0 (3) and 72.5 (2)° with the 2,4-dichloropheny and 2,4-difluorophenyl rings, respectively, and the molecule adopts a Z-conformation about the C=C double bond. In the crystal, C—H⋯O and C—H⋯N hydrogen bonds link the molecules.
1,4-Dimethoxy-2,5-bis{2-[4-(trifluoromethyl)phenyl]ethynyl}benzene

The asymmetric unit of the title compound, C26H16F6O2, contains one half of the molecule situated on an inversion centre. In the rod-like molecule, the two terminal benzene rings form a dihedral angle of 71.9 (1)° with the central benzene ring. The trifluoromethyl group is rotationally disordered over two orientations in a 0.53 (1):0.47 (1) ratio. The crystal packing exhibits no classical intermolecular interactions.
4-[(5-Chloro-2-hydroxybenzylidene)amino]-3-ethyl-1H-1,2,4-triazole-5(4H)-thione

The title compound, C11H11ClN4OS, crystallizes with two molecules, A and B, in the asymmetric unit in which the dihedral angles between the triazole and benzene rings are 54.6 (3) and 56.0 (3)°. Both molecules feature an intramolecular O—H⋯N hydrogen bond, which generates an S(6) ring. In the crystal, A–B dimers are linked by pairs of weak C—H⋯S hydrogen bonds along with π–π stacking interactions between the triazole rings [centroid–centroid separations = 3.631 (3) and 3.981 (4)Å]. N—H⋯S hydrogen bonds link the dimers into [100] chains, which feature R 2 2(8) loops.
7-Diethylamino-3-{(E)-4-[(E)-2-(pyridin-4-yl)ethenyl]styryl}-2H-chromen-2-one

In the title coumarin derivative, C28H26N2O2, the coumarin unit is approximately planar, with a maximum deviation of 0.048 (3) Å. The central benzene ring is oriented at dihedral angles of 30.15 (14) and 10.51 (11)°, respectively, to the pyridine ring and coumarin ring system. In the crystal, weak C—H⋯O and C—H⋯N hydrogen bonds and weak C—H⋯π interactions link the molecules into a three-dimensional supramolecular architecture.
Carbonylchlorido(1-methylsulfanylpenta-1,3-dien-1-yl-5-ylidene)bis(triphenylphosphane)osmium(II)

The crystal structure of the title compound, [Os(C6H7S)Cl(C18H15P)2(CO)], confirms the formulation as an osmabenzene. There is a slightly distorted octahedral coordination environment at the OsII ion, with the triphenylphosphane ligands mutually trans and the chloride cis to the carbon bearing the –SMe substituent. Within the metallacyclic ring, the C—C distances are appropriate for aromatic bonds and the two Os—C distances are shorter than typical Os—C single bonds. The maximum deviation from the least-squares plane through the osmabenzene ring occurs for the carbon bearing the SMe substituent [0.1037 (18) Å].
Revisión de los métodos de evaluación de la actividad antioxidante in vitro del vino y valoración de sus efectos in vivo.

effects. The aim of this paper is to evaluate the significance of these methods, as well as to correlate the antioxidant activity of wines with their phenolic profile, both in qualitative and quantitative terms. Red wines show higher antioxidant capacities than white ones and the magnitude of these differences depends on the method used. The antioxidant activity of wine can not be mainly ascribed to a particular phenolic compound, instead it is explained by the global interaction of all of them. To evaluate the influence of red wine consumption in the human organism, plasma antioxidant capacity has been frequently used as biomarker, and studies have shown that it increases after wine ingestion. We can conclude that it is necessary to use a battery of methods that provide different and complementary information to properly interpret the results. Phenolic compounds undergo metabolic transformations in the organism which modify their activities. In vivo assays do consider these changes. From the studies performed up to date we can conclude that acute ingestion of wine directly acts on plasma antioxidant capacity due to phenolic compounds and indirectly influences by means of changes on plasmatic concentration of endogenous antioxidants.
2-[3,5-Bis(4-methoxyphenyl)-4,5-dihydro-1H-pyrazol-1-yl]-4,6-bis(4-methoxyphenyl)pyrimidine

In the title compound, C35H32N4O4, the pyrazole ring forms a dihedral angle of 15.04 (8)° with the adjacent pyrimidine ring. The pyrimidine ring forms dihedral angles of 9.95 (8) and 1.86 (7)° with its adjacent methoxy-substituted benzene rings, whereas the equivalent angles are 80.24 (9) and 11.55 (9)° for the pyrazole ring and its adjacent benzene rings. The crystal packing features π–π interactions, the centroid–centroid distance between the pyrimidine and methoxyphenyl rings being 3.604 (1) Å. The pyrazole ring is nearly planar, with a maximum deviation of 0.020 (3) Å for the –CH2– carbon.
5-Dimethylamino-N,N-dimethyl-2-nitrobenzamide

In the title compound, C11H15N3O3, one of the methyl groups attached to the benzamide unit is slightly twisted with a C—N—C—C torsion angle of 4.04 (13)°. The crystal packing is stabilized by weak intermolecular C—H⋯O hydrogen bonds together with a weak C—H⋯π interaction.
{3-[(2-Chloro-1,3-thiazol-4-yl)methyl]-1,3-thiazolidin-2-ylideneamino}formonitrile

In the title compound, C8H7ClN4S2, the dihedral angle between the thiazolidine ring (r.m.s. deviation = 0.028 Å) and the thiazole ring (r.m.s. deviation = 0.004 Å) is 74.74 (6)°. The formonitrile group is almost coplanar with the attached ring [C—N—C—N torsion angle = 167 (2)°.
(E)-2-[2-(3-Nitrophenyl)ethenyl]quinolin-8-ol

In the title compound, C17H12N2O3, the mean planes of the benzene ring and the quinoline moiety are inclined to one another by 11.0 (1)°. The nitro substituent is twisted at an angle of 7.9 (2)° with respect to the attached benzene ring. Intramolecular O—H⋯N and C—H⋯N hydrogen bonds occur. The crystal is constructed of molecular stacks without involvement of π-stacking interactions, but showing interstack association via O—H⋯O and C—H⋯O hydrogen bonding. Thus, the supramolecular architecture of the crystal results from stacked molecules stabilized by hydrogen bonding between the stacks.
5-(Hydroxymethyl)furan-2-carbaldehyde

The title compound (HMF), C6H6O3, is one of the products of acid-catalyzed dehydration of high-fructose corn syrup, and has been shown to be toxic to honey bees. The compound was crystallized at 276 K, and it was found that the two independent molecules in the asymmetric unit form an infinite O—H⋯O hydrogen-bonding chain that is linked into a three-dimensional network structure by weak intermolecular C—H⋯O contacts.
5-(4-Hydroxyphenyl)imidazolidine-2,4-dione

The title compound, C9H8N2O3, was prepared by reaction of phenol, glyoxylic acid and urea in water. The imidazolidine ring adopts an almost planar conformation (r.m.s. deviation = 0.012 Å) and is twisted by 89.3 (1)° relative to the benzene ring. In the crystal, molecules are linked by N—H⋯O and O—H⋯O hydrogen bonds into a three-dimensional framework.
[5-Chloro-2-hydroxy-N′-(2-oxidobenzylidene)benzohydrazidato]pyridinecopper(II)

In the title complex, [Cu(C14H9ClN2O3)(C5H5N)], the CuII ion exhibits a distorted trans-CuN2O2 square-planar geometry arising from the O,O,N-tridentate ligand and a pyridine molecule. An intramolecular O—H⋯N hydrogen bond occurs. In the crystal structure, weak intermolecular C—H⋯π interactions generate a chain. The crystal studied was an inversion twin.
15-Methoxy-14,15-dihydroandranginine

The title polycyclic alkaloid, C22H26N2O3, an indole derivative obtained from Melodinus yunnanensis, comprises three chiral C atoms and crystallizes as a racemate. Its seven-membered heterocyclic ring has a twisted conformation, with the N atom within the plane of the indole moiety and with two adjacent C atoms deviating in opposite directions from its plane by 0.756 (3) (methylene C) and −0.802 (3) Å (methine C). In the crystal, pairs of N—H⋯O hydrogen bonds connect the molecules into centrosymmetric dimers.
4-[(2-Chloro-4-nitrophenyl)diazenyl]-N,N-diethylaniline

In the title compound, C16H17ClN4O2, the aromatic ring is twisted slightly with respect to the plane of the diazene group [N—N—C—C torsion angle = −3.9 (4)°]. The NO2 group is twisted by 16.2 (4)° relative to the aromatic ring. The two ethyl chains are positioned such that one ethyl chain lies above and the other below the ring.
1-Phenyl-1-[(1-phenylethyl)sulfonylmethylsulfonyl]ethane

There are two molecules in the asymmetric unit of the title compound, C17H20O4S2. There are slight differences in the twist of the two rings relative to the S–C–S chain [dihedral angles of 48.41 (18) and 87.58 (16)° in the first molecule and 45.98 (18) and 87.02 (18)° in the second] and the difference in the C—S—C—S torsion angles [176.68 (17) and −77.6 (2)° for the two independent molecules].
2,2′-(Ethane-1,2-diyl)bis(1H-benzimidazole)

The complete molecule of the title compound, C16H14N4, is generated by crystallographic inversion symmetry. In the crystal, molecules are linked by N—H⋯N hydrogen bonds, generating (001) sheets. Weak aromatic π–π stacking interactions [centroid–centroid distances = 3.7383 (13) and 3.7935 (14) Å] are also observed.
(5-Bromo-2-hydroxyphenyl)(4-propylcyclohexyl)methanone

In the title compound, C16H21BrO2, the cyclohexane ring adopts a chair conformation. The hydroxy and carbonyl groups are involved in an intramolecular O—H⋯O hydrogen bond. In the crystal, weak C—H⋯O interactions link the molecules into zigzag chains along [010].
4,4′-[2,2-Dimethylpropane-1,3-diylbis(nitrilomethylidyne)]dibenzonitrile

The title compound, C21H20N4, is a bidentate Schiff base ligand. An intramolecular C—H⋯N hydrogen bond forms a five-membered ring, producing an S(5) ring motif. The cyano and imino [–C(H2)—N=C–] functional groups are coplanar with the benzene ring in each half of the molecule. The packing of the molecules is controlled by C—H⋯π and π–π interactions [centroid-to-centroid distance = 3.6944 (8) Å].
6-Methoxy-2-[(E)-phenyliminomethyl]phenol

The title compound, C14H13NO2, was obtained by the condensation reaction of o-vanillin and aniline in ethanol. The molecule adopts the phenol–imine tautomeric form and an E conformation with respect to the azomethine C=N bond. The dihedral angle between the aromatic rings is 30.57 (10)°. In the crystal, molecules are linked by intermolecular C—H⋯O hydrogen bonds into zigzag chains parallel to the b axis.
Articular and periarticular fractures in the dog and cat

ARTICULAR and periarticular fractures are common in the dog and cat and, from the point of view of treatment, are often very unforgiving injuries. Failure to deal with these fractures appropriately may result in the development of significant degenerative joint disease (osteoarthritis) and periarticular fibrosis and adhesions, which, in turn, may lead to permanent loss of joint and limb function. Open reduction and internal fixation is generally indicated, and postoperative care is an extremely important aspect of management. This article reviews the various types of articular and periarticular fractures, and discusses the management and prognosis of the most common of these injuries in dogs and cats (with the exception of distal limb fractures and fractures involving shear injuries). Postoperative care techniques and potential complications are also described.
1,6-Bis(diphenylarsino)hexane

The title diphenylarsino compound, C30H32As2 or Ph2As(CH2)6AsPh2, lies about a crystallographic inversion centre located at the mid-point of the central Csp 3—Csp 3 bond of the methylene chain. The two benzene rings bonded to As are inclined to one another at a dihedral angle of 75.98 (8)°. In the crystal structure, weak intermolecular C—H⋯π interactions stack the molecules down the b axis.
Dichloridobis[2-(2-chloroethyl)-1,2,3,4-tetrahydropyrazino[1,2-a]benzimidazole-κN]cobalt(II)

In the title compound, [CoCl2(C12H14ClN3)2], the central CoII ion lies on a twofold rotation axis and adopts a distorted tetrahedral coordination geometry defined by two N atoms from two 2-(2-chloroethyl)-1,2,3,4-tetrahydropyrazino[1,2-a]benzimidazole ligands and two chloride anions. The Cl atom located in the side chain of the ligand is involved in intermolecular C—H⋯Cl hydrogen bonding, which links neutral complex units into a one-dimensional right-handed helical chain running along a crystallographic 41 axis. Such hydrogen-bonded helical chains are connected to each other to form a homochiral three-dimensional supramolecular network. One C atom of the 2-chloroethyl chain is disordered over two positions, with site-occupancy factors of 0.52 and 0.48.
Incidência de complicações locais no cateterismo venoso periférico e fatores de risco associados

Objective To estimate the incidence of local complications associated with peripheral catheters and identify risk factors for the development of most common complications. Methods This prospective cohort study included 92 adult inpatients at clinical and surgical units who had peripheral catheterization. By daily observance of the catheters we determined time of permanence and local complications due to the use of a complete safety catheter. All actions began after training of nursing teams. Statistical tests used were the Fisher exact test, G test (Williams), chi-square, Mann-Whitney U test, and relative risk. Results Local complications occurred in 56.2% of cases. Time of catheter permanence over 72 hours increased the risk for phlebitis development in 2.34% of cases (RR; p=0.0483; CI [0.91; 6.07]). Conclusion Incidence of local complications was high. Phlebitis was the predominant complication and the time of catheter permanence over 72 hours was a considered risk factor for this complication.
Bis(4-{2-[4-(diethylamino)phenyl]ethenyl}pyridine-κN)diiodidozinc

In the title compound, [ZnI2(C17H20N2)2], the ZnII atom is four-coordinated by two I atoms and the N atoms of two pyridine rings belonging to different ligands in a distorted tetrahedral geometry. The coordinating pyridine rings are oriented in an almost perpendicular fashion, making a dihedral angle of 83.7 (5)°.
Understanding-in-the-large

Developing and maintaining large industrial software products implies programming-in-the-large activities. Related concepts are usually represented in terms of low level features such as file system hierarchies, preprocessor files, makefiles, shell scripts, sccs archives, etc. Understanding the information embedded in such artifacts is an important but difficult task, especially with neither conceptual framework, nor tool assistance. To emphasize the importance of this issue, this paper makes the distinction between understanding-in-the-large and understanding-in-the-small. Using a conceptual classification, understanding-in-the-large problems are described in a structured way. Difficulties in building reverse-engineering-in-the-large tools are then analyzed and illustrated taking preprocessor files as a case study. The Champollion approach to these problems is briefly presented.
(1R,2R)-N,N′-Diisobutyl-N,N′-dimethylcyclohexane-1,2-diamine

The title compound, C16H34N2, is a chiral diamine with fixed R configuration at both stereogenic carbon centres of the cyclohexane backbone. Due to their different substituents, the two N atoms also become stereogenic. In the crystal structure, the configuration at one of the two nitrogen centres is fixed, with the free electron pair pointing inward and the isobutyl group in a trans position towards the cyclohexane backbone resulting in an R configuration. The isobutyl group at the second N atom, however, is disordered with 75% S configuration and 25% R configuration. In both cases, the isobutyl group is arranged in a trans position towards the cyclohexane backbone.
Detection of Mycoplasma orale contamination in cell culture by PCR method

IJMM 1392; 7(2): P 7-14 :فادها و هنیمز يگدولآ تشک اه ی اهامسلاپوکيام طسوت يلولس هلئسم یا مهم روانف رد ی تشک بوسحم يلولس يم دوش زا ييلااب نازيم . تشک اه ی هب هدولآ يلولس هلاروا امسلاپوکيام دنتسه هک ًامومع طسوت نانکراک تروص هاگشيامزآ م ي دريگ سانش نياربانب . ييا خشت و صي هب نآ زا یريگولج روظنم ز ناي یاه نيا اب يگدولآ لابند هب مهم ناگمادنازير اه (Microorganism) تيمها زئاح رايسب تسا نيا فده . هعلاطم هار زادنا ی و هب گراک یري يلپ هريجنز شنکاو شور هب زارم شور ناونع ي يصاصتخا و ساسح ،عيرس صيخشت یارب هلاروا امسلاپوکيام رد گدولآ ي یاه و يلولس تشک هدروارف اه ی تسيز يتخانش تسا . :راک شور و داوم اب هب گراک یري درادناتسا هيوس هلاروا امسلاپوکيام ، تست PCR تفج زا هدافتسا اب هنوگ يصاصتخا یاهرميارپ هلاروا امسلاپوکيام نژ هيحان رد 16S rRNA هار زادنا ی . ديدرگ 26 هنومن تشک لآ هب کوکشم يلولس هاگشيامزآ هب يعاجرا امسلاپوکيام يگدو سنرفر زا سپ یزار هسسوم امسلاپوکيام جارختسا DNA شيامزآ دروم PCR . دنتفرگ رارق هتفاي اه : تست PCR هار زادنا ی اب هدش DNA امسلاپوکيام هلاروا شنکاو یاراد تبثم يتروص رد ،دوب رياس اب هک هنوگ اه ی :ريظن امسلاپوکيام لاپوکيام مويراويلاس امس ، امسلاپوکيام سينيرويه ، امسلاپوکيام