Miracle-dz/UniFAR-FTUs huggingface dataset:
RNA determinants of junction site selection in RNA virus recombinants and defective interfering RNAs.

RNA recombination plays an important role in the diversification and evolution of RNA viruses. Most of these events are believed to be mediated by an actively copying viral replicase switching from a donor template to an acceptor template, where it resumes synthesis. In addition, intramolecular replicase-mediated events (i.e., rearrangements) can lead to the generation of replicable deleted forms of a viral genome, termed defective interfering (DI) RNAs. To gain further insight into the recombination process, the effect of various primary and secondary structures on recombination site selection in vivo was examined using plant RNA tombusviruses. The effect of sequence identity and complementarity on deletion events that generate DI RNAs was also investigated. Our results suggest that (1) 5' termini and strong hairpin structures in donor templates represent preferred sites for recombinations, (2) junction sites in acceptor templates do not occur in double-stranded regions, (3) nucleotide homology can shift donor and acceptor recombination sites closer to regions of identity and, (4) both sequence identity and complementarity can direct deletion sites in DI RNAs. These results further define RNA determinants of tombusvirus RNA recombination and rearrangement.
Short-term Memory Impairment after Isoflurane in Mice Is Prevented by the &agr;5 &ggr;-Aminobutyric Acid Type A Receptor Inverse Agonist L-655,708

Background:Memory blockade is an essential component of the anesthetic state. However, postanesthesia memory deficits represent an undesirable and poorly understood adverse effect. Inhibitory &agr;5 subunit–containing &ggr;-aminobutyric acid subtype A receptors (&agr;5GABAA) are known to play a critical role in memory processes and are highly sensitive to positive modulation by anesthetics. We postulated that inhibiting the activity of &agr;5GABAA receptors during isoflurane anesthesia would prevent memory deficits in the early postanesthesia period. Methods:Mice were pretreated with L-655,708, an &agr;5GABAA receptor–selective inverse agonist, or vehicle. They were then exposed to isoflurane for 1 h (1.3%, or 1 minimum alveolar concentration, or air-oxygen control). Then, either 1 or 24 h later, mice were conditioned in fear-associated contextual and cued learning paradigms. In addition, the effect of L-655,708 on the immobilizing dose of isoflurane was studied. Motor coordination, sedation, anxiety, and the concentration of isoflurane in the brain at 5 min, 1 h, and 24 h after isoflurane were also examined. Results:Motor and sensory function recovered within minutes after termination of isoflurane administration. In contrast, a robust deficit in contextual fear memory persisted for at least 24 h. The &agr;5GABAA receptor inverse agonist, L-655,708, completely prevented memory deficits without changing the immobilizing dose of isoflurane. Trace concentrations of isoflurane were measured in the brain 24 h after treatment. Conclusions:Memory deficits occurred long after the sedative, analgesic, and anxiolytic effects of isoflurane subsided. L-655,708 prevented memory deficit, suggesting that an isoflurane interaction at &agr;5GABAA receptors contributes to memory impairment during the early postanesthesia period.
Towards application of text mining for enhanced power network data analytics - Part II: Offline analysis of textual data

Text mining is a subdivision of data mining technologies used to extract useful information from unstructured textual data. In recent years, power distribution networks have become more complex due to the versatile consumer demand and integration of distributed energy resources. This has led to the need for enhanced data processing and analysis, i.e., data analytics, in distribution system studies. This paper for the first time explores the feasibility of application of text mining methods as a part of power system data analytics. The focus is on identifying and describing the steps that need to be taken for the knowledge extraction from large offline textual document collections and on demonstrating the effectiveness of the whole process if undertaken by a power system engineer, i.e., a nonspecialist in the area of text mining.
Recruiting for Small Business Growth : Micro-Level Evidence

We examine the link between new employees in leading positions and subsequent productivity in small- and medium-sized (SME) enterprises. Managers and professionals are likely to possess important tacit knowledge. They are also in a position to influence the employing firm. Exploiting rich and comprehensive panel data for Sweden in the 2001-2010 period and employing semi-parametric and quasi-experimental estimation techniques, we find that newly recruited leading personnel have a positive and statistically significant impact on the productivity of the hiring SME. Interestingly, our results suggest that professionals with experience from international firms and enterprise groups contribute the most to total factor productivity. Overall, the findings suggest the importance of mobility of leading personnel for productivity-enhancing knowledge spillovers to SMEs.
A new approach for biased affine estimation

The problem of biased affine estimation is discussed in the present paper. Affine estimation is a technique for improving parameter estimation through the inclusion of a-priori information in a non-bayesian setting. Here, a new optimality criterion for affine estimation is presented and developed. The closed form optimal transformation for this criterion is obtained through the use of the KKT conditions, as the new criterion is posed as a convex optimization problem. This new criterion is compared with other affine estimation criteria through a numerical example.
Approximate Gaussian Density Evolution Based Analysis of Distributed and Adaptive Turbo Codes

In this paper, the non-selective Decode-and-Forward (DF) scheme proposed in is analyzed using approximate Gaussian density evolution (DE). Based on the DE analysis, performance is shown to be improved by adaptively changing the transmit code at the relay node in response to the channel conditions. Simulation results show that the proposed adaptive scheme improves performance, particularly when the relay is closer to the destination than to the source. In addition, the adaptive scheme does not increase network overhead.
An examination of the factors affecting people’s participation in future health examinations based on community health exam interventions

Community-based intervention health examinations were implemented at a health care facility to comply with the government’s primary health care promotion policy. The theory of planned behavior model was applied to examine the effect that community-based health examinations had on people’s health concepts regarding seeking future health examinations. The research participants were individuals who had received a health examination provided at two branches of a hospital in central Taiwan in 2012. The hospital’s two branches held a total of 14 free community-based health examination sessions. The hospital provided health examination equipment and staff to perform health examinations during public holidays. We conducted an exploratory questionnaire survey to collect data and implemented cross-sectional research based on anonymous self-ratings to examine the public’s intention to receive future community-based or hospital-based health examinations. Including of 807 valid questionnaires, accounting for 89.4% of the total number of questionnaires distributed. The correlation coefficients of the second-order structural model indicate that attitudes positively predict behavioral intentions (γ = .66, p < .05), and subjective norms also positively predict behavioral intentions (γ = .66, p < .01). By contrast, perceived behavioral control has no significant relationship with behavioral intentions (γ = −.71, p > .05). The results of the first-order structural model indicated that the second-order constructs had a high explanatory power for the first-order constructs. People’s health concepts regarding health examinations and their desire to continue receiving health examinations must be considered when promoting health examinations in the community. Regarding hospital management and the government’s implementation of primary health care, health examination services should address people’s medical needs to increase coverage and participation rates and reduce the waste of medical resources.
AN OBJECT-ORIENTED APPROACH FOR HYBRID SYSTEM MODELLING

Abstract This paper presents a novel modelling approach for hybrid industrial plants. It is based on the introduction of object-oriented concepts to the Differential Predicate Transition nets. To handle the system complexity a top-down methodology is considered, where class models are successively refined and decomposed. The UML language is also used to represent different views of the modelled system. A cane sugar factory is used as an example to illustrate the proposed approach.
Quasi-linear evolution of tearing modes

The growth of a tearing instability in Rutherford's non-linear regime is investigated. Using a singular perturbations technique, we recover to lowest order Rutherford's result. To the following order we show that the mode generates a quasi-linear deformation of the equilibrium flux profile, whose resistive diffusion slows down the growth and shows the possibility of a saturation of the instability Etude de la croissance d'une instabilite de dechirure dans le regime non lineaire de Rutherford. L'usage des techniques de perturbations singulieres permet de trouver a l'ordre le plus bas, les resultats de Rutherford. A l'ordre suivant on montre que le mode engendre une deformation quasi lineaire du profil d'equilibre du flux magnetique; la diffusion resistive de cette deformation ralentit la croissance du mode et indique la possibilite d'une saturation de l'instabilite
Noncircadian Regulation and Function of Clock Genes Period and Timeless in Oogenesis of Drosophila Melanogaster

Circadian clock genes are ubiquitously expressed in the nervous system and peripheral tissues of complex animals. While clock genes in the brain are essential for behavioral rhythms, the physiological roles of these genes in the periphery are not well understood. Constitutive expression of the clock gene period was reported in the ovaries of Drosophila melanogaster; however, its molecular interactions and functional significance remained unknown. This study demonstrates that period(per) and timeless(tim) are involved in a novel noncircadian function in the ovary. PER and TIM are constantly expressed in the follicle cells enveloping young oocytes. Genetic evidence suggests that PER and TIM interact in these cells, yet they do not translocate to the nucleus. The levels of TIM and PER in the ovary are affected neither by light nor by the lack of clock-positive elements Clock(Clk) and cycle(cyc). Taken together, these data suggest that per and tim are regulated differently in follicle cells than in clock cells. Experimental evidence suggests that a novel fitness-related phenotype may be linked to noncircadian expression of clock genes in the ovaries. Mated females lacking either per or tim show nearly a 50% decline in progeny, and virgin females show a similar decline in the production of mature oocytes. Disruption of circadian mechanism by either the depletion of TIM via constant light treatment or continuous expression of PER via GAL4/UAS expression system has no adverse effect on the production of mature oocytes.
ensity and phase equilibrium for ice and tructure I hydrates using the Gibbs – Helmholtz onstrained equation of state

A new, rigorous framework centered around the multi-scale GHC equation of state is presented for predicting bulk density and phase equilibrium for light gas–water mixtures at conditions where hexagonal ice and structure I hydrate phases can exist. The novel aspects of this new framework include (1) the use of internal energies of departure for ice and empty hydrate respectively to determine densities, (2) contributions to the standard state fugacity of water in ice and empty hydrate from lattice structure, (3) computation of these structural contributions to standard state fugacity from compressibility factors and EOS parameters alone, and (4) the direct calculation of gas occupancy from phase equilibrium. Numerical results for densities and equilibrium for systems involving ice and/or gas hydrates predicted by this GHC-based framework are compared to predictions of other equations of state, density correlations, and experimental data where available. Results show that this new GHC-based EOS framework accurately predicts the densities of hexagonal water ice and structure I gas hydrates as well as phase equilibrium for methane–water and CO2–water mixtures. © 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
2.4GHz WLAN RF energy harvester for passive indoor sensor nodes

This paper presents the design and measurement results of an RF Energy Harvester aimed to power sensor nodes like temperature, humidity, chemical, or radiation in an indoor industrial or residential environment. The harvester operates at 2.42 GHz WiFi-WLAN frequency band. It consists of multiple microstrip patch antennas, power combiner, voltage quadruple Greinacher rectifier circuit, and a super capacitor to store the harvested energy. All elements are designed using low-loss Rogers RO3206 substrate. The impedance matching of the power combiner with a rectifier is a non-trivial issue due to change in diode impedance with the input power. The peak efficiency is measured to be 57.8% at 6 to 8dBm input power. In the presence of realistic -10dBm continuous signal, the system can charge a 33mF super capacitor to 1.6V in 20 minutes. This collected energy is enough to power 10mW sensor node for a period of more than 4 seconds to perform wake up, sense and transmit functions, and put a sensor back to sleep mode.
Control of the acute phase response. Demonstration of C-reactive protein synthesis and secretion by hepatocytes during acute inflammation in the rabbit

To determine the cell of origin of C-reactive protein (CRP) and to cast light on the mechanisms leading to the acute phase response, we used an immunoenzymatic technique to visualize this protein in livers from rabbits at intervals after intramuscular injection of turpentine. CRP was detected only in hepatocytes. 8 h after turpentine injection, CRP was demonstrated in occasional periportal hepatocytes. With time, larger numbers of positive cells were detected successively in perilobular, midlobular, and centrilobular areas. On electron microscopy, CRP was detected in rough endoplasmic reticulum (RER), smooth endoplasmic reticulum (SER), and Golgi apparatus (GA). When colchicine was administered to inhibit cellular secretion of CRP, intensity of reaction and number of CRP-containing hepatocytes were substantially greater than without colchicine, but the sequence of intralobular distribution was similar. At peak serum response 38 h after turpentine injection, CRP could be demonstrated in most hepatocytes. Electron microscopic studies showed accumulation of CRP on membranes and lumina of RER, SER, GA, and in cytoplasmic vacuoles. These findings indicate that CRP is produced by progressively increasing numbers of hepatocytes after inflammatory stimulus and suggest that a mediator, acting initially in portal zones, is responsible for recruitment of cells to CRP production.
Compact Wideband Phase Shifter Using Microstrip Self-Coupled Line and Broadside-Coupled Microstrip/CPW for Multiphase Feed-Network

In this letter, a compact wideband phase shifter using a microstrip self-coupled line and a broadside-coupled microstrip/CPW (BCMC) structure is proposed for multiphase feed-network. With a uniform phase reference, such a phase shifter can achieve multiphase responses by adjusting instinct self- and broadside couplings for constant phase shifts within a wideband. Then, with the combination of proposed phase shifters and a microstrip Wilkinson power divider, a multiphase feed-network can be implemented. To verify the mechanisms mentioned earlier, a wideband feed-network is fabricated with measured multiphase responses (i.e., 0°, 5.625°, 11.25°, 22.5°, 45°, 90°, and 180°) and maximum insertion loss of 1.57 dB from 1.7 to 2.3 GHz.
A Classification-Based Virtual Machine Placement Algorithm in Mobile Cloud Computing

In recent years, cloud computing services based on smart phones and other mobile terminals have been a rapid development. Cloud computing has the advantages of mass storage capacity and high-speed computing power, and it can meet the needs of different types of users, and under the background, mobile cloud computing (MCC) is now booming. In this paper, we have put forward a new classification-based virtual machine placement (CBVMP) algorithm for MCC, and it aims at improving the efficiency of virtual machine (VM) allocation and the disequilibrium utilization of underlying physical resources in large cloud data center. By simulation experiments based on CloudSim cloud platform, the experimental results show that the new algorithm can improve the efficiency of the VM placement and the utilization rate of underlying physical resources.
Disruption of Transplant Tolerance by an “Incognito” Form of CD8 T Cell–Dependent Memory

Several approaches successfully achieve allograft tolerance in preclinical models but are challenging to translate into clinical practice. Many clinically relevant factors can attenuate allograft tolerance induction, including intrinsic genetic resistance, peritransplant infection, inflammation, and preexisting antidonor immunity. The prevailing view for immune memory as a tolerance barrier is that the host harbors memory cells that spontaneously cross‐react to donor MHC antigens. Such preexisting “heterologous” memory cells have direct reactivity to donor cells and resist most tolerance regimens. In this study, we developed a model system to determine if an alternative form of immune memory could also block tolerance. We posited that host memory T cells could potentially respond to donor‐derived non‐MHC antigens, such as latent viral antigens or autoantigens, to which the host is immune. Results show that immunity to a model nonself antigen, ovalbumin (OVA), can dramatically disrupt tolerance despite undetectable initial reactivity to donor MHC antigens. Importantly, this blockade of tolerance was CD8+ T cell–dependent and required linked antigen presentation of alloantigens with the test OVA antigen. As such, this pathway represents an unapparent, or “incognito,” form of immunity that is sufficient to prevent tolerance and that can be an unforeseen additional immune barrier to clinical transplant tolerance.
Clutch Size in the Tropical Scincid Lizard Emoia sanfordi, a Species Endemic to the Vanuatu Archipelago

Abstract The majority of species in the scincid genus Emoia (Squamata: Scincidae) have a fixed clutch size of two eggs per clutch and produce between two and four clutches per year. One lineage within Emoia, the Emoia samoensis species group, consists of 13 species occurring in Melanesia and the islands of the southwestern Pacific Ocean, and exhibits variation in clutch size, with previously reported clutch sizes of two to five eggs. Little is known about reproduction in several members of this lineage including Emoia sanfordi, a large-bodied lizard endemic to the archipelago of Vanuatu in the South Pacific. We analyzed reproduction and clutch size in E. sanfordi females and discovered that there is a substantial amount of intraspecific variation, with clutch size ranging from two to seven eggs, with a modal clutch size of five eggs. Females were reproductively active throughout the study period of June through October and appear to be laying multiple clutches. The variation in clutch size seen in E. sanfordi is congruent with the variation previously reported within other closely related species.
A Quantitative Study of General Dental Practitioner Orthodontic Care in Scottish Health Board Areas (1966–1975)

A quantitative assessment of the true dental practitioner contribution to orthodontic care in Scottish Health Board areas during the period 1966–1975, shows that the amount of orthodontic treatment given by general dental practitioners in Scotland has diminished in the 10 year period studied. A wide variation was observed in respect of the mean number of orthodontic treatments per year per practitioner, and there was also a lack of uniformity in the proportion of practitioners providing an orthodontic service relative to the different area health boards. The findings have also shown that between 1966–1975, a considerable reduction has occurred in the mean number of practitioner orthodontic treatments commenced each year in the Greater Glasgow and Lothian Health Board areas. The evidence of this study also suggests that orthodontics should become recognized as a postgraduate dental specialty and that it would be appropriate to introduce specialist registration as a means of improving the quality and quantity of orthodontic care within the National Health Service. This should also be accompanied by certain administrative changes, as the present anomalous situation whereby specialist practitioners are permitted to practise within the general dental service should be discontinued.
Contribution of the dimeric state to the thermal stability of the flavoprotein D‐amino acid oxidase

The flavoenzyme DAAO from Rhodotorula gracilis, a structural paradigm of the glutathione‐reductase family of flavoproteins, is a stable homodimer with a flavin adenine dinucleotide (FAD) molecule tightly bound to each 40‐kD subunit. In this work, the thermal unfolding of dimeric DAAO was compared with that of two monomeric forms of the same protein: a Δloop mutant, in which 14 residues belonging to a loop connecting strands βF5–βF6 have been deleted, and a monomer obtained by treating the native holoenzyme with 0.5 M NH4SCN. Thiocyanate specifically and reversibly affects monomer association in wild‐type DAAO by acting on hydrophobic residues and on ionic pairs between the βF5–βF6 loop of one monomer and the αI3′ and αI3″ helices of the symmetry‐related monomer. By using circular dichroism spectroscopy, protein and flavin fluorescence, activity assays, and DSC, we demonstrated that thermal unfolding involves (in order of increasing temperatures) loss of tertiary structure, followed by loss of some elements of secondary structure, and by general unfolding of the protein structure that was concomitant to FAD release. Temperature stability of wild‐type DAAO is related to the presence of a dimeric structure that affects the stability of independent structural domains. The monomeric Δloop mutant is thermodynamically less stable than dimeric wild‐type DAAO (with melting temperatures (Tms) of 48°C and 54°C, respectively). The absence of complications ensuing from association equilibria in the mutant Δloop DAAO allowed identification of two energetic domains: a low‐temperature energetic domain related to unfolding of tertiary structure, and a high‐temperature energetic domain related to loss of secondary structure elements and to flavin release.
A security concept for OLAP

A data warehouse collects and integrates data from multiple, autonomous, heterogeneous sources with the purpose of efficiently implementing decision support or OLAP queries. Much working data warehousing has been performed on view materialization and data integration, we focus on access and security management in OLAP and N-dimensional cube. Since data in data warehouse are valuable and an important cooperate resource, we define a security model for data warehouses which describes security constrains for roles in the data warehouse. Each user in the data warehouse has a role and each role has a security constrain list that builds the security profile of the role. According these role profile the user is authorized to query data from the data warehouse.