Miracle-dz/UniFAR-FTUs huggingface dataset:
Manual wheelchair propulsion cost across different components and configurations during straight and turning maneuvers

Aim Maneuvering manual wheelchairs is defined by changes in momentum. The amount of effort required to maneuver a wheelchair is dependent on many factors, some of which reflect the design and configuration of the wheelchair. Objective The objective of this study was to measure the work required to propel a manual wheelchair configured with three weight distributions, three drive wheels and four casters. Methods A novel wheelchair-propelling robot was used as the test platform to measure work while traversing two surfaces using three different maneuvers which were defined to highlight different kinetic energies and energy loss mechanisms. Results Overall, propulsion cost decreased with an increase in load on the drive wheels. Pneumatic drive wheels exhibited lower propulsion costs compared to a solid tire. Two casters, a 4″ dia × 1.5″ and a 5″ dia × 1″, exhibited better overall performance compared to 5″ dia × 1.5″ solid and 6″ dia × 1″ pneumatic casters. Discussion The results indicate that drive wheel load and types of drive wheels and casters impact propulsion cost and their influences differ across maneuvers and surfaces. The approach is well suited to assess equivalency in components and configurations. Assessment of performance equivalency would empower clinicians and users with important knowledge when selecting components.
Harmonization efforts add value to clinical laboratory and improve patient safety

Laboratory tests provide a basis for medical decisions; for this reason they are of great relevance to healthcare, both in clarifying diagnoses, in prescribing or monitoring treatment, and in making decisions about hospitalization or hospital discharges. Reliability of test results depends on technical quality, the technology that must be employed within strict specifications, the use of materials and supplies of good origin, efficiency and implementation of mechanisms for potential failure prevention.
PERCEIVED STRESS IN UNDERGRADUATE PHYSICAL THERAPY STUDENTS OF PESHAWAR, PAKISTAN

Background: Stress results from different kind of stressors that affect students either positively ornegatively depending on their coping ability. Academic stressors are the main cause of stress anddepression among undergraduate allied health sciences’ students. The aim of this study was todetermine the prevalence of perceived stress and its association with gender among undergraduatephysical therapy students of Peshawar. Methodology: Level of perceived stress was assessed usingcross sectional study design at different Physiotherapy institutes of Peshawar, Pakistan. A total of 300students were recruited from five different institutes. Degree and prevalence of stress was assessedusing Perceived stress scale (Cohen PSS 10). Results: Seventy-two percent students were found to bemoderately stressed, 23.3% were highly stressed whereas 4.7% had low level of stress. Female studentsshowed significantly higher stress levels (23.50±4.80 vs 21.50±5.60, p=0.01). Control on irritation andconfidence were considered as positive stressors whereas nervousness, stress and loss of patience werefound to be the important negative stressors. Conclusion: A significant number of physiotherapystudents fall in moderate to high stress categories with female preponderance.
Potential Use of Sofosbuvir in the Prophylaxis for Rabies

1 Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John’s University, Queens, NY, United States, 2 Departments of Pathology and Obstetrics and Gynecology and Women’s Health, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY, United States, 3 Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, University of Toledo, Toledo, OH, United States
Alcohol use disorder increases the risk of necrotizing fasciitis

Abstract This nationwide retrospective cohort study determined the association between alcohol use disorder (AUD) and the risk of necrotizing fasciitis (NF). This study used health insurance claims data of 52,212 in-patients with AUD and 208,848 controls randomly frequency-matched by age and sex at a 1:4 ratio. The AUD cohort included patients newly diagnosed with AUD between January 1, 2000 and December 31, 2008. The NF event occurrence was observed until December 31, 2011. We used the Kaplan–Meier method to present the cumulative incidence curve and Cox proportional hazard models to depict the risk of NF in patients with AUD. The incidence of NF was 19.4 per 10,000 person-years in the AUD cohort, which was nearly 7.73-fold higher than that in the comparison cohort (2.54 per 10,000 person-years). After adjustment for age, sex, and comorbidities, the patients with AUD exhibited a 3.55-fold higher risk of NF than did the controls (hazard ratio [HR] = 3.55, 95% confidence interval [CI] = 3.00–4.20). Nevertheless, in the AUD groups without any comorbidity, patients with AUD exhibited a significant 15.2-fold higher risk of NF than did the comparison cohort (HR = 15.2, 95% CI = 10.9–21.3). Moreover, the adjusted HRs of NF risk with respect to the severity of AUD were 2.15 (95% CI = 1.76–2.62), 4.54 (95% CI = 3.67–5.62), and 10.7 (95% CI = 8.66–13.2) for mild, moderate, and severe AUD, respectively. This study indicated that AUD should be considered an independent and significant risk factor for NF.
Versatile workflow for cell type–resolved transcriptional and epigenetic profiles from cryopreserved human lung

Complexity of lung microenvironment and changes in cellular composition during disease make it exceptionally hard to understand molecular mechanisms driving development of chronic lung diseases. Although recent advances in cell type–resolved approaches hold great promise for studying complex diseases, their implementation relies on local access to fresh tissue, as traditional tissue storage methods do not allow viable cell isolation. To overcome these hurdles, we developed a versatile workflow that allows storage of lung tissue with high viability, permits thorough sample quality check before cell isolation, and befits sequencing-based profiling. We demonstrate that cryopreservation enables isolation of multiple cell types from both healthy and diseased lungs. Basal cells from cryopreserved airways retain their differentiation ability, indicating that cellular identity is not altered by cryopreservation. Importantly, using RNA sequencing and EPIC Array, we show that gene expression and DNA methylation signatures are preserved upon cryopreservation, emphasizing the suitability of our workflow for omics profiling of lung cells. Moreover, we obtained high-quality single-cell RNA-sequencing data of cells from cryopreserved human lungs, demonstrating that cryopreservation empowers single-cell approaches. Overall, thanks to its simplicity, our workflow is well suited for prospective tissue collection by academic collaborators and biobanks, opening worldwide access to viable human tissue.
Parkinson's Disease Diagnosis Using Neostriatum Radiomic Features Based on T2-Weighted Magnetic Resonance Imaging

Background: Parkinson's disease (PD) is a neurodegenerative disease in which the neostriatum, including the caudate nucleus (CN) and putamen (PU), has an important role in the pathophysiology. However, conventional magnetic resonance imaging (MRI) lacks sufficient specificity to diagnose PD. Therefore, the study's aim was to investigate the feasibility of using a radiomics approach to distinguish PD patients from healthy controls on T2-weighted images of the neostriatum and provide a basis for the clinical diagnosis of PD. Methods: T2-weighted images from 69 PD patients and 69 age- and sex-matched healthy controls were obtained on the same 3.0T MRI scanner. Regions of interest (ROIs) were manually placed at the CN and PU on the slices showing the largest respective sizes of the CN and PU. We extracted 274 texture features from each ROI and then used the least absolute shrinkage and selection operator regression to perform feature selection and radiomics signature building to identify the CN and PU radiomics signatures consisting of optimal features. We used a receiver operating characteristic curve analysis to assess the diagnostic performance of two radiomics signatures in a training group and estimate the generalization performance in the test group. Results: There were no significant differences in the demographic and clinical characteristics between the PD patients and healthy controls. The CN and PU radiomics signatures were built using 12 and 7 optimal features, respectively. The performance of the two radiomics signatures to distinguish PD patients from healthy controls was good. In the training and test groups, the AUCs of the CN radiomics signatures were 0.9410 (95% confidence interval [CI]: 0.8986–0.9833) and 0.7732 (95% CI: 0.6292–0.9173), respectively, and the AUCs of the PU radiomics signature were 0.8767 (95% CI: 0.8066–0.9469) and 0.7143 (95% CI: 0.5540–0.8746), respectively. Vertl_GlevNonU_R appeared simultaneously in both the CN and PU radiomics signatures as an optimal feature. A t-test analysis revealed significantly higher levels of texture values of the CN and PU in the PD patients than healthy controls (P < 0.05). Conclusion: Neostriatum radiomics signatures achieved good diagnostic performance for PD and potentially could serve as a basis for the clinical diagnosis of PD.
Analysis of Mrgprb2 Receptor-Evoked Ca2+ Signaling in Bone Marrow Derived (BMMC) and Peritoneal (PMC) Mast Cells of TRPC-Deficient Mice

Mast cells are a heterogeneous group of immune cells. The simplest and commonly accepted classification divides them in two groups according to their protease content. We have compared the action of diverse secretagogues on bone marrow derived (BMMC) and peritoneal (PMC) mast cells which represent classical models of mucosal and connective tissue type mast cells in mice. Whereas, antigen stimulation of the FcεRI receptors was similarly effective in triggering elevations of free intracellular Ca2+ concentration ([Ca2+]i) in both BMMC and PMC, robust [Ca2+]i rise following Endothelin-1 stimulation was observed only in a fraction of BMMC. Leukotriene C4 activating cysteinyl leukotriene type I receptors failed to evoke [Ca2+]i rise in either mast cell model. Stimulation of the recently identified target of many small-molecule drugs associated with systemic pseudo-allergic reactions, Mrgprb2, with compound 48/80, a mast cell activator with unknown receptor studied for many years, triggered Ca2+ oscillations in BMMC and robust [Ca2+]i rise in PMCs similarly to that evoked by FcεRI stimulation. [Ca2+]i rise in PMC could also be evoked by other Mrgprb2 agonists such as Tubocurarine, LL-37, and Substance P. The extent of [Ca2+]i rise correlated with mast cell degranulation. Expression analysis of TRPC channels as potential candidates mediating agonist evoked Ca2+ entry revealed the presence of transcripts of all members of the TRPC subfamily of TRP channels in PMCs. The amplitude and AUC of compound 48/80-evoked [Ca2+]i rise was reduced by ~20% in PMC from Trpc1/4/6−/− mice compared to Trpc1/4−/− littermatched control mice, whereas FcεRI-evoked [Ca2+]i rise was unaltered. Whole-cell patch clamp recordings showed that the reduction in compound 48/80-evoked [Ca2+]i rise in Trpc1/4/6−/− PMC was accompanied by a reduced amplitude of Compound 48/80-induced cation currents which exhibited typical features of TRPC currents. Together, this study demonstrates that PMC are an appropriate mast cell model to study mechanisms of Mrgprb2 receptor-mediated mast cell activation, and it reveals that TRPC channels contribute at least partially to Mrgprb2-mediated mast cellactivation but not following FcεRI stimulation. However, the channels conducting most of the Ca2+ entry in mast cells triggered by Mrgprb2 receptor stimulation remains to be identified.
Morphological Estimation of Cellularity on Neo-Adjuvant Treated Breast Cancer Histological Images

This paper describes a methodology that extracts morphological features from histological breast cancer images stained for Hematoxilyn and Eosin (H&E). Cellularity was estimated and the correlation between features and the residual tumour size cellularity after a Neo-Adjuvant treatment (NAT) was examined. Images from whole slide imaging (WSI) were processed automatically with traditional computer vision methods to extract twenty two morphological parameters from the nuclei, epithelial region and the global image. The methodology was applied to a set of images from breast cancer under NAT. The data came from the BreastPathQ Cancer Cellularity Challenge 2019, and consisted of 2579 patches of 255×255 pixels of H&E histopatological samples from NAT treatment patients. The methodology automatically implements colour separation, segmentation and morphological analysis using traditional algorithms (K-means grouping, watershed segmentation, Otsu’s binarisation). Linear regression methods were applied to determine strongest correlation between the parameters and the cancer cellularity. The morphological parameters showed correlation with the residual tumour cancer cellularity. The strongest correlations corresponded to the stroma concentration value (r = −0.9786) and value from HSV image colour space (r = −0.9728), both from a global image parameters.
DNA replication and chromosome positioning throughout the interphase in three dimensional space of plant nuclei

Despite the recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains limited. In this study, DNA replication timing and interphase chromosome positioning was analyzed in seven Poaceae species differing in genome size. A multidisciplinary approach combining newly replicated DNA labelling by EdU, nuclei sorting by flow cytometry, three-dimensional immuno-FISH, and confocal microscopy revealed similar replication timing order for telomeres and centromeres as well as for euchromatin and heterochromatin in all seven species. The Rabl configuration of chromosomes that lay parallel to each other and their centromeres and telomeres are localized at opposite nuclear poles, was observed in wheat, oat, rye and barley with large genomes, as well as in Brachypodium with a small genome. On the other hand, chromosomes of rice with a small genome and maize with relatively large genome did not assume proper Rabl configuration. In all species, the interphase chromosome positioning inferred from the location of centromeres and telomeres was stable throughout the interphase. These observations extend earlier studies indicating a more complex relation between genome size and interphase chromosome positioning, which is controlled by factors currently not known. Highlight Telomere and centromere replication timing and interphase chromosome positioning in seven grass species differing in genome size indicates a more complex relation between genome size and the chromosome positioning.
A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property. Materials and Methods: Twenty-five adult (8–10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology. Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated. Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.
Genome-Wide Identification, Phylogeny, and Expressional Profiles of the Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Pyropia yezoensis

Mitogen-activated protein kinase kinase kinases (MAPKKKs) are pivotal components of MAPK cascades. Pyropia yezoensis, belonging to Rhodophyta, is an important macroalga growing in the intertidal zone of temperate Japan and China. At present, little is known about MAPKKKs and their potential regulatory mechanisms in response to environmental stressors in red algae, particularly with regard to how this gene family retained its ancient characteristics. In this study, we identified 33 MAPKKK genes in P. yezoensis by bioinformatics analysis. Phylogenetic analysis classified them into Raf, MEKK and ZIK groups, which contained conserved kinase motif with minor variations. The number of intron of all PyMAPKKK is few, up to 3. Regulatory elements prediction indicated that the cis-acting elements included many motifs involved in the stress response. Furthermore, expression analysis of PyMAPKKKs under different stress conditions revealed that numerous PyMAPKKK genes were involved in various signaling pathways with different expression patterns. Additionally, 16 PyMAPKKK genes were validated their expression patterns by Realtime-PCR analysis. An interactome network of PyMAPKKK was constructed to show that most of the interacting proteins were serine/threonine protein kinases, MAPKs, and ubiquitin-binding proteins. Our study provided helpful information for understanding the evolution and function of the MAPKKK family in P. yezoensis.
Modeling Morphological Priming in German With Naive Discriminative Learning

Both localist and connectionist models, based on experimental results obtained for English and French, assume that the degree of semantic compositionality of a morphologically complex word is reflected in how it is processed. Since priming experiments using English and French morphologically related prime-target pairs reveal stronger priming when complex words are semantically transparent (e.g., refill–fill) compared to semantically more opaque pairs (e.g., restrain–strain), localist models set up connections between complex words and their stems only for semantically transparent pairs. Connectionist models have argued that the effect of transparency should arise as an epiphenomenon in PDP networks. However, for German, a series of studies has revealed equivalent priming for both transparent and opaque prime-target pairs, which suggests mediation of lexical access by the stem, independent of degrees of semantic compositionality. This study reports a priming experiment that replicates equivalent priming for transparent and opaque pairs. We show that these behavioral results can be straightforwardly modeled by a computational implementation of Word and Paradigm Morphology (wpm), Naive Discriminative Learning (ndl). Just as wpm, ndl eschews the theoretical construct of the morpheme. Ndl succeeds in modeling the German priming data by inspecting the extent to which a discrimination network pre-activates the target lexome from the orthographic properties of the prime. Measures derived from an ndl network, complemented with a semantic similarity measure derived from distributional semantics, predict lexical decision latencies with somewhat improved precision compared to classical measures, such as word frequency, prime type, and human association ratings. We discuss both the methodological implications of our results, as well as their implications for models of the mental lexicon.
HUMAN PANCREATIC CANCER CELLS UNDERGO PROFOUND METABOLIC REPROGRAMMING TOWARDS CELLULAR STEMNESS AS ADAPTATION TO INHIBITION OF THE AKT PATHWAY

Cancer cells acquire resistance to cytotoxic therapies targeting major survival pathways by adapting their metabolism. The AKT pathway is a major regulator of human pancreatic adenocarcinoma progression. The mechanisms of adaptation to long-term silencing of AKT isoforms of pancreatic cancer cells were studied. Following silencing, cancer cells remained quiescent for long periods of time, after which they recovered proliferative capacities. Adaptation caused profound proteomic changes largely affecting mitochondrial biogenesis, energy metabolism, and acquisition of a number of distinct cancer stem cell (CSC) characteristics depending on the AKT isoform that was silenced. The adaptation to AKT1 silencing drove most de-differentiation and acquisition of stemness through C-MYC down-modulation and NANOG up-regulation, which were required for survival of adapted CSCs. The changes associated to adaptation sensitized cancer cells to inhibitors targeting regulators of oxidative respiration and mitochondrial biogenesis. In vivo pharmacological co-inhibition of AKT and mitochondrial metabolism effectively controlled pancreatic adenocarcinoma growth in pre-clinical models.
Processing Method Altered Mouse Intestinal Morphology and Microbial Composition by Affecting Digestion of Meat Proteins

Our previous study showed that the intake of meat proteins dynamically affected fecal microbial composition. However, the digestion of processed meat proteins in vivo and its relationship with gut microbiota and host remain unclear. In this study, we collected cecal contents and intestinal tissue from the mice fed with casein, soybean protein (SP), and four processed pork proteins for 8 months, and analyzed the amino acid (AA) files, cecum microbial composition and metabolites, and intestinal morphology. Dry-cured pork protein and stewed pork protein (SPP) groups had significantly higher total AA content in gut content than the other groups, but the content of the SPP group was relatively lower in the serum. The microbial composition of the processed meat protein groups differed from the casein or SP group, which is consistent with changes in AA composition. Emulsion sausage protein and SP diets upregulated the microbial AA metabolism, energy metabolism, signaling molecules and interaction, translation, and digestive system function but downregulated the microbial membrane transport, signal transduction and cell motility function compared to the casein diet. The SPP diets increased concentrations of acetate, propionate, butyrate, and isovalerate by specific gut microbes, but it decreased the relative abundance of Akkermansia. Moreover, the mice fed SP diet had relatively lower crypt depth, higher villus height and V/C ratio in duodenum, with the longer small intestines and the heavier cecum than other diets. These results suggested that processing methods altered bioavailability of meat proteins, which affected the intestinal morphology and the cecum microbial composition and function.