Miracle-dz/UniFAR-FTUs huggingface dataset:
Growth performance of Acacia mangium provenances in Parung Panjang, Bogor and its correlation with physical and mechanical wood properties

Wood quality and growth are two important parameters that need to be analyzed for the utilization and selection of superior genotypes. The objective of this research was to investigate the ten provenances growth characteristics and their physical and mechanical properties of Acacia mangium at Parung Panjang, Bogor. The provenance trial was arranged in three blocks of a randomized completely blocks design. In every provenance, 18 trees were sampled resulting in a total of 180 trees in each block representing each provenance measured for their total height, clear bole height, stem diameter at breast height, stem form, branching system, and crown form. Three individual trees were selected randomly from each provenance for wood properties traits, i.e. moisture content, density, modulus of elasticity (MOE) and modulus of rapture (MOR). Significant differences among provenances were detected for stem diameter, clear bole height, branching system and crown form, while total height and stem form revealed not significantly different. Most of growth parameters was not significantly correlated with physical and mechanical wood traits, except for MOR, it revealed significant correlation with the total tree height, stem form, and branching system. Generally, some growth characteristics were not a good indicator for selecting the best provenance in wood properties. The best growth performance was revealed by Kiriwo/Serisa WP, Bimadebun Village, and Balimo District, but from wood quality, the superior provenance was revealed by Bimadebun Village with the lower moisture content, the highest wood density, the highest MOE and MOR. The Bimadebun Village provenance is potential to be developed as provenance seed stand, especialy for solid (contruction) wood.
Identification of wild hosts of tomato yellow leaf curl virus in South-Eastern Iran

Abstract In this research, to identify natural wild hosts of TYLCV, 44 symptomatic samples were collected in or around harvested tomato fields in south-eastern Iran and tested for the TYLCV infection by PCR. Among four PCR-positive plant species, full-length genome of TYLCV was amplified by rolling circle amplification (RCA) method only from a ground cherry (Physalis divaricata L., Solanaceae) sample. Cloning and full-length genome sequencing of a ground cherry isolate (G4) showed that it comprises 2756 nucleotides (nts) in length and shares 87.7%–95.2% nt sequence identities with TYLCV isolates reported from Iran, Oman and Pakistan. The G4 isolate of TYLCV was successfully transmitted to tomato plants and the original host (P. divaricata) via agroinoculation and showed typical symptoms. According to the results of this research, four symptomatic weed species appear to be alternative hosts of TYLCV and play the role of the primary inoculum for the viral infection.
Dynamic shifts of visual and saccadic signals in prefrontal cortical regions 8Ar and FEF

Active vision is a fundamental process by which primates gather information about the external world. Multiple brain regions have been studied in the context of simple active vision tasks in which a visual target’s appearance is temporally separated from saccade execution. Most neurons have tight spatial registration between visual and saccadic signals, and in areas such as prefrontal cortex (PFC) some neurons show persistent delay activity that links visual and motor epochs and has been proposed as a basis for spatial working memory. Many PFC neurons also show rich dynamics, which have been attributed to alternative working memory codes and the representation of other task variables. Our study investigated the transition between processing a visual stimulus and generating an eye movement in populations of PFC neurons in macaque monkeys performing a memory guided saccade task. We found that neurons in two subregions of PFC, the frontal eye fields (FEF) and area 8Ar, differed in their dynamics and spatial response profiles. These dynamics could be attributed largely to shifts in the spatial profile of visual and motor responses in individual neurons. This led to visual and motor codes for particular spatial locations that were instantiated by different mixtures of neurons, which could be important in PFC’s flexible role in multiple sensory, cognitive, and motor tasks. New and Noteworthy A central question in neuroscience is how the brain transitions from sensory representations to motor outputs. The prefrontal cortex contains neurons that have long been implicated as important in this transition and in working memory. We found evidence for rich and diverse tuning in these neurons, that was often spatially misaligned between visual and saccadic responses. This feature may play an important role in flexible working memory capabilities.
The complete chloroplast genome of Blepharis ciliaris (Acanthaceae)

Abstract Blepharis ciliaris is an important medicinal plant and endemic species in Saudi Arabia. This study reported the complete chloroplast genome of B. ciliaris, the second to be sequence in non cystolith clade of Acanthaceae. The genome is 149, 717 bp in size and consisted of a pair of inverted repeat (25, 331 bp each) separating the two single copy region, the large single copy (LSC) 82, 057 bp and small single copy SSC 16, 998 bp. The plastome has overall GC content of 38.5% and 112 genes comprising of 79 protein coding genes, 30 tRNA genes and 4 rRNA genes. The phylogenetic relationship analysis showed that B. ciliaris is sister to Aphelandra knappiea. The cp genome reported in this study will useful in genetic diversity and evolutionary studies of the species.
A REVIEW ARTICLE ON: SUPERDISINTEGRANTS

Disintegration has play a major role in improving the drug activity and hence increases the patient compatibility. The therapeutic activity of the formulations is obtained by disteintegration followed by dissolution. The inclusion of right disintegrants is a prerequisite to get optimal bioavailability in tablets and capsules. Disintegrants are the substances that causes the rapid disintegration of the capsules or tablets into smaller particles that dissolves more rapidly than in the absence of the disintegrants. On the other hand superdisintegrants ,as it name suggests superior to disintegrants are the substances which facilitates or increases the disintegration time even at low level ,typically 1-10% by weight relative to the total weight of the dosage unit. This article comprises of study of superdisintegrants which are being used in the formulation to provide safe and effective drug delivery with improved patient compliance.
Morphological differences among phyllosoma larvae of the pronghorn spiny lobster, Panulirus penicillatus, from the western, central, and eastern areas of the Pacific Ocean

̶ The pronghorn spiny lobster, Panulirus penicillatus , is known to have the widest distribution among palinurid lobster species, occurring in tropical and sub-tropical areas of the Indo-Paciﬁc Ocean. In the Paciﬁc Ocean, mitochondrial DNA analyses have revealed that the western–central and eastern populations are genetically isolated. We performed morphological investigations of mid- to late-stage phyllosoma larvae collected in western, central, and eastern areas of the Paciﬁc Ocean. The larvae from the western area had a signiﬁcantly longer cephalic shield, shorter abdo -men, and longer eyestalk than those from the eastern area. Additionally, for larger lar vae, the widest part of the cephalic shield in larvae from the western area was located closer to the middle of the median line of the cephalic shield than in larvae from the eastern area. The ratio of width to length of cephalic shield and the ratio of cephalic shield width to thorax width are key traits for distinguishing between the larvae from the western and eastern areas. The morphology of larvae from the central area tended to be similar to that of larvae from the western
Overlap in synaptic neurological condition susceptibility pathways and the neural pannexin 1 interactome revealed by bioinformatics analyses

Many neurological conditions exhibit synaptic impairments, suggesting mechanistic convergence. Additionally, the pannexin 1 (PANX1) channel and signalling scaffold is linked to several of these neurological conditions and is an emerging regulator of synaptic development and plasticity; however, its synaptic pathogenic contributions are relatively unexplored. To this end, we explored connections between synaptic neurodevelopmental disorder and neurodegenerative disease susceptibility genes discovered by genome-wide association studies (GWASs), and the neural PANX1 interactome (483 PANX1-interacting proteins identified from mouse Neuro2a cells). To identify shared susceptibility genes, we compared synaptic suggestive GWAS candidate genes amongst autism spectrum disorders, schizophrenia, Parkinson’s disease, and Alzheimer’s disease. Next, to further probe PANX1 signalling pathways at the synapse, we used bioinformatics tools to identify PANX1 interactome signalling pathways and protein-protein interaction clusters. To shed light on synaptic disease mechanisms potentially linking PANX1 and these four neurological conditions, we performed additional cross-analyses between gene ontologies enriched for the PANX1 synaptic and disease-susceptibility gene sets. Finally, to explore the regional specificity of synaptic PANX1-neurological conditions connections, we identified brain region-specific elevations of synaptic PANX1 interactome and GWAS candidate gene set transcripts. Our results confirm considerable overlap in risk genes for autism spectrum disorders and schizophrenia and identify potential commonalities in genetic susceptibility for neurodevelopmental disorders and neurodegenerative diseases. Our findings also pinpointed novel putative PANX1 links to synaptic disease-associated pathways, such as regulation of vesicular trafficking and proteostasis, warranting further validation.
The underestimated taxa: the role of non-bee pollinators in temperate vegetable crops, experimental research in strawberry (Fragaria spp.) crops

THE UNDERESTIMATED TAXA: THE ROLE OF NON-BEE POLLINATORS IN TEMPERATE VEGETABLE CROPS, EXPERIMENTAL RESEARCH IN STRAWBERRY (Fragaria spp.) CROPS Ellen Richard University of Guelph, 2019 Advisor(s): Dr. Nigel E. Raine Dr. Dirk Steinke Pollination services are critical to agricultural systems, providing a third of global food production. Non-bee pollinators have received little recognition with regards to their role in commercial agricultural pollination. Diverse pollinator communities often provide better pollination services, and non-bee pollinators represent 95% of this diversity. Additionally, research demonstrates that many non-bee pollinators are more resilient to land use intensification and climate change due to their nomadic life-history and tolerance to inclement weather. The aim of this thesis is two-fold. It demonstrates the diversity of non-bee insects that visit temperate vegetable crops in a comprehensive review. Secondly, it presents research on the non-bee floral visiting community of dayneutral strawberries in Southern-Ontario. Using barcoding methods as well as quantitative analysis it characterises flower visitor communities, their foraging preferences and levels of floral fidelity. Hoverflies were found to be important non-bee flower visitors, carrying comparable amounts of pollen to bees.
Epidemiological study of the patients referred for thalassemia diagnosis using chorionic villous sampling (CVS) in Genetic Laboratory of Dastgheib Hospital, Shiraz, 2011

Objective: Pre-natal diagnosis is the most effected way to prevent genetic diseases in a society. The aim of this research was to show the prevention level of the birth of the children with major thalassemia disorder and the demographic condition of the people referring to the Shahid Dastgheib Genetic Center in Shiraz for the pre natal diagnosis. Materials and methods: The present research was a cross- sectional (descriptive, analytical) one. In this study, the amount of sampling was done by census in a way that all the case (372 cases) related to the year 2010. The questionnaire was prepared based on the information present in the files. In order to compare the quantitative and qualitative variables, two sample t - test and K sample t- test were used. Results: Out of 372 fetuses tested, 25.5% had major thalassemia, 48.7% minor thalassemia, 0.8% intermediate, 1.3% sickle cell, and 23.7% were healthy. All the cases diagnosed with thalassemia were introduced for abortion, and abortion was carried out. Major thalassemia was more prevalent in Lore tribes (32.9%), which was more in comparison to the members of others tribes. Conclusion: In order to prevent major thalassemia, it is important to identify the gene carriers and prevent their marriage. Nevertheless, in many places in the country, especially in the villages and rural areas, the couples do the experiment after they have already gotten emotionally involved and made the arrangements to get married; therefore they're unwilling to stop the marriage. As a result, post-nuptial CVS during pregnancy is crucial.
Comparison of carcass traits, meat quality and expressions of MyHCs in muscles between Mashen and Large White pigs

Abstract The aim of this study was to evaluate the differences in carcase traits, meat quality and muscle characteristics (mean fibre diameter and MyHCs levels of expression) between Mashen and Large White pigs. A total of 60 pigs (30 Mashen pigs and 30 Large White pigs, half male and half female in each breed) were used in this experiment. Results showed that Mashen pigs exhibited lower slaughter weight, reduced back-fat thickness, reduced loin eye area and lower lean meat ratio than Large White pigs (p < .05). Meat from Large White pigs had higher pH1, greater drip losses, higher Warner-Bratzler shear force values, higher lightness, higher yellowness, and greater mean diameter of muscle fibres. Also, meat from Large white pigs had lower cooked yield, lower redness, and reduced intramuscular fat content than meat from Mashen pigs (p < .05). The contents of 17 amino acids, total amino acids, essential amino acids and umami amino acids in the longissimus dorsi of Mashen pigs were higher than those in Large White pigs (p < .05). Large White pigs had higher monounsaturated fatty acids and lower polyunsaturated fatty acids than Mashen pigs (p < .05). Meanwhile, the expression of MyHCI was significantly higher and the expression of MyHCIIb was lower in Mashen than in Large White pigs (p < .05). The higher MyHCI and lower MyHCIIb found in muscles from Mashen pigs might partially explain meat quality differences found between the two breeds in the present study. These results provide valuable information for meat quality differences between Mashen and Large White pigs. Highlights Carcase traits and meat quality between Large White and Mashen pigs exhibited significant differences. The total amino acids, essential amino acids and umami amino acids in Mashen pigs were higher than those in Large White pigs. Large White pigs had higher monounsaturated fatty acids and lower polyunsaturated fatty acids than Mashen pigs. The expression of MyHCI was significantly higher and the expression of MyHCIIb was lower in Mashen than in Large White pigs.
Bacteriophages—a new hope or a huge problem in the food industry

Bacteriophages are viruses that are ubiquitous in nature and infect only bacterial cells. These organisms are characterized by high specificity, an important feature that enables their use in the food industry. Phages are applied in three sectors in the food industry: primary production, biosanitization, and biopreservation. In biosanitization, phages or the enzymes that they produce are mainly used to prevent the formation of biofilms on the surface of equipment used in the production facilities. In the case of biopreservation, phages are used to extend the shelf life of products by combating pathogenic bacteria that spoil the food. Although phages are beneficial in controlling the food quality, they also have negative effects. For instance, the natural ability of phages that are specific to lactic acid bacteria to destroy the starter cultures in dairy production incurs huge financial losses to the dairy industry. In this paper, we discuss how bacteriophages can be either an effective weapon in the fight against bacteria or a bane negatively affecting the quality of food products depending on the type of industry they are used.
Diversity of bacterial communities in the rhizosphere of the endangered plant, Paeonia jishanensis

The microbial community in the rhizosphere is thought to provide plants with a second set of genomes, which plays a pivotal role in plant growth. In the present study, soil samples were collected from the rhizosphere of an endangered plant, Paeonia jishanensis. The plants were divided into three groups: well-growing plants, poor-growing plants and dead plants. Metagenomic DNA was isolated from rhizosphere soil samples of these plants and 16S rRNA genes were sequenced by the PacBio-RS II system. The results of taxonomic analysis showed that the dominant phyla were Proteobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Actinobacteria in all three sample types. Linear discriminate analysis Effect Size (LEfSe) showed that 5 species, Hirschia baltica, Arcobacter aquimarinus, Gimesia maris, Magnetococcus marinus and Pseudoxanthobactor soli, were significantly enriched in the rhizosphere of well-growing plants. Additionally, the results of PCA, MDS and clustering analysis indicated that the bacterial community in the rhizosphere of living P. jishanensis plants was similar. With the death of plants, the bacterial community changed considerably. These findings suggest that the abundance of many beneficial rhizospheric microbes declined with the death of P. jishanensis. This is a potential way to preserve endangered plants by inoculating declining species with beneficial microbes.
Interactive Effects of Food Supplementation and Snake Fungal Disease on Pregnant Pygmy Rattlesnakes and Their Offspring

Abstract. In viviparous organisms, the ability to feed while pregnant may mitigate energetic trade-offs experienced during the reproductive process and enhance fecundity. However, anorexia during pregnancy has been reported in many crotaline snakes. The potential costs and benefits of feeding while pregnant are not completely described in the literature, and experimental studies have been conducted in a limited number of taxa, rendering our understanding of the forces that may underlie the evolution of anorexia in pregnant snakes incomplete. Here, we examine the impact of food supplementation during mid to late pregnancy on mothers and offspring in a viviparous crotaline snake species, the Pygmy Rattlesnake (Sistrurus miliarius). Specifically, we offered multiple large meals to mothers held in outdoor enclosures and measured a suite of maternal and offspring traits including maternal body condition, offspring length and mass, maternal disease state, and offspring foraging behaviors. We focused on interactions between feeding, clinical signs of snake fungal disease (SFD), and the presence of its causative agent, Ophidiomyces ophiodiicola, in mothers. Pregnant females fed readily when offered food, but feeding did not impact offspring traits. Food supplementation significantly increased maternal postparturient body condition, but also increased clinical signs of disease in mothers and led to a significantly higher reproductive failure rate in the treatment group, particularly in mothers afflicted with SFD. Our results suggest that food supplementation during pregnancy may disrupt the reproductive process in pregnant rattlesnakes, and that such disruption is particularly pronounced in mothers suffering from SFD.
PPARD CC and ACTN3 RR genotype prevalence among elite soccer players

ABSTRACT Objectives: Soccer is a complex sport in which players are required to perform variety of aerobic and anaerobic activities. The purpose of the study was to explore aerobic (PPARD CC) and anaerobic (ACTN3 RR) performance-related genotype frequencies among young soccer players and compare to those of sprinters/jumpers (S/J), long distance runners (LDR) and controls. Methods: Genomic DNA was extracted from buccal epithelial cells of 170 national level athletes (60 soccer players, 51 S/J, 59 LDR) and 51 non-athletic controls . Genotypes were determined using Taqman allelic discrimination assay. Results: Soccer players had non-significant lower frequency (15%) of the PPARD 294CC genotype compared to LDR (19%), but higher compared to S/J (10%). ACTN3 R577X genotype and allele frequencies of soccer players (28%) were not significantly different from those of S/J (39%)and LDR athletes (20%). Conclusions: Soccer players do not have a noticeable or clear genotype tendency compared to S/J and LDR. This may suggest that soccer players rely more on diverse physiological qualities as well as other factors that are required in the game, rather than on a single physiological attribute. It should be also noticed that the different physiological demands for diverse positional roles in soccer may contribute to the results’ heterogeneity.
The RGG Domain of the Yeast Nucleolin Nsr1 Is Required for the Genome Instability Associated with Co-transcriptionally Formed G4 DNA

A significant increase in genome instability is associated with the conformational shift of a guanine-run-containing DNA strand into the four-stranded G4 DNA. Until recently, the mechanism underlying the recombination and genome rearrangements following the formation of G4 DNA in vivo has been difficult to elucidate but has become better clarified by the identification and functional characterization of several key G4 DNA-binding proteins. Mammalian nucleolin NCL is a highly specific G4 DNA-binding protein with a well-defined role in the transcriptional regulation of genes with associated G4 DNA-forming sequence motifs at their promoters. The consequence of the in vivo interaction between G4 DNA and nucleolin in respect to the genome instability has not been previously investigated. We show here that G4 DNA-binding is a conserved function in the yeast nucleolin Nsr1. Furthermore, we demonstrate that the Nsr1-G4 DNA complex formation is a major factor in inducing the genome instability associated with the co-transcriptionally formed G4 DNA in the yeast genome. The G4-associated genome instability and the G4 DNA-binding in vivo requires the arginine-glycine-glycine (RGG) repeats located at the C-terminus of the Nsr1 protein. Nsr1 with the deletion of RGG domain supports normal cell growth and is sufficient in in vitro G4 DNA binding as well as protection from the cytotoxicity of a G4 ligand TMPyP4. However, this deletion results in abrogation of in vivo binding to the co-transcriptionally formed G4 DNA and in severe reduction in the G4-assicated genome instability. Our data suggest that the interaction between Nsr1 with intact RGG repeats and G4 DNA impairs genome stability by precluding the access of G4-resolving proteins and obstructing replication.