Miracle-dz/UniFAR-FTUs huggingface dataset:
Is It High Time to Increase Elite Soccer Substitutions Permanently?

Rules determine how team sport matches occur. Match-induced fatigue is specific to each sport, and may be associated with injury incidence. For example, the injury rate in soccer is distinctly higher during matches than in training sessions. Understanding the differences between team sports rules might be useful for enhancing rules (e.g., safer sport). Therefore, this study aimed to evaluate the impact of the rule-induced physical demands between soccer, futsal, basketball, and handball, focusing on substitution rules. Data from the elite team sports’ rules (e.g., absolute and relative court dimensions; the number of players, substitutions allowed, total game time, time-outs) were collected, including the changes due to the coronavirus disease (COVID-19) pandemic in soccer substitutions, and comparisons were performed. The data showed that soccer has higher rule-induced physical demands: e.g., substantially lower substitution rate, higher dimensions in absolute (eight to fifteen times), and relative (four to eight times) values. Simulations also showed that soccer has extremely large differences, even considering COVID-19 substitution changes (from three to up to five). We conclude that elite soccer has remarkably higher overall rule-induced physical demands than elite futsal, basketball and handball, and increasing soccer substitutions permanently (e.g., unlimited) might mitigate overall soccer demands.
Hepatic veno-occlusive disease in allogeneic stem cell transplant recipients with prior exposure to gemtuzumab ozogamicin or inotuzumab ozogamicin

Abstract Hepatic veno-occlusive disease (VOD/sinusoidal obstructive syndrome) represents a constellation of clinical findings including right upper quadrant pain, jaundice, hepatomegaly, and ascites. In the post-hematopoietic stem cell transplant (SCT) setting, the reported incidence has been 10–15%, with severe VOD historically resulting in high mortality rates. Novel agents including calicheamicin conjugated with CD33 (gemtuzumab ozogamicin; GO) and CD22 (inotuzumab ozogamicin; InO) are increasingly used for the treatment of acute myeloid leukemia and acute lymphoblastic leukemia, respectively. Both GO and InO are highly active, but also have unique hepatotoxicity profiles, including a higher risk of VOD in recipients of SCT. Introduction of GO and InO into pre-SCT leukemia management adds additional complexity to SCT patient selection and toxicity monitoring. In this article, we describe and review the risks and management associated with VOD in SCT recipients exposed to GO and InO.
ISOLATION OF PROTEASE ENZYME FROM CHAYOTE FRUIT (Sechium edule (Jacq.) Sw.) WITH AMMONIUM SULFATE FRACTINATION METHOD

Protease is an enzyme that is capable to hydrolyze (breakdown) protein molecules intosimpler compounds such as small peptides and amino acids. The aim of the research was toisolate protease enzyme from chayote (Sechium edule (Jacq.) Sw. ) using fractinationammonium sulfate method and to find out the optimum saturation level of the ammoniumsulfate. P rotease activity examination of each fraction of ammonium sulfate was performedusing Anson method. Protein content assay was determined using Biuret method. The resultsshowed that crude extract protease of chayote had specific activity of 3,7338 x 10-3 U/mg. Theoptimal saturation levels of ammonium sulfate for protease chayote precipitation was 40-50%. At this saturation level, the highest enzyme spesific activity were 16,00 x 10-3 U/mg,with four times purifying of protease enzyme from the crude extract protease.
Isolation and identification of canine parvovirus type 2b in Korean dogs

Abstract : Canine parvovirus (CPV) is a major diarrhea-causing agent in puppies. Since CPV type 2 (CPV-2) emergedin 1978, new antigenic variants including CPV-2a, CPV-2b, and CPV-2c have been identified in many countries. Twopuppies died suddenly at a veterinary clinic in Gyeonggi province, South Korea. Two viruses were isolated in A72cells, confirmed as CPV strains based on a CPV rapid kit and an indirect fluorescence test and designated QIACP1403and QIACP1404. The nucleotide sequences of complete VP2 genes of QIACP1403 and QIACP1404 were determined,and the corresponding amino acid sequences were deduced. Molecular analyses revealed that the QIACP1403 andQIACP1404 isolates were type CPV-2b. Several mutated amino acids were detected on VP2 gene residues of the twoisolates. Phylogenetic analyses showed that the two isolates were most closely related to strain CPV-BM11, whichwas isolated from Chinese dogs in 2011. Our results suggest that these isolates may be a candidate for a vaccineto prevent CPV infection in dogs after conducting passages of the isolates in an in vitro culture system.Keywords : CPV-2b, canine parvoviral enteritis, virus isolation
Molecular characterization of the cucumber necrosis virus coat protein gene

A cucumber necrosis tombusvirus (CNV) mutant which lacks the coding sequence for the coat protein protruding domain, PD(-), was constructed by site-directed mutagenesis of an infectious CNV cDNA clone, pK2/M5 (wild-type, 4701 nt). Transcripts of PD(-) were infectious in Nicotiana clevelandii, however, the resulting local lesions were significantly smaller than those on the corresponding leaves of plants inoculated with wild-type transcript. In addition, systemic symptoms took 8 to 12 days longer to develop than in wild type-inoculated plants. N. clevelandii inoculated with sap from systemically infected leaves of PD(-) transcriptinoculated plants showed local lesions the same as wild-type and the delay in systemic symptom development was either reduced or, occasionally, lost. Systemic symptoms in both PD(-) transcriptand sap-inoculated N. clevelandii were either similar to the systemic necrosis of wild-type or an oak-leaf necrosis was observed. High molecular weight RNA from mutantand wild-typeinfected plants was extracted and analyzed by Northern blotting. Full-length PD(-) RNA could be detected only rarely in RNA preparations from transcript-inoculated leaves; a further deleted, stable RNA species of approximately 3800 nucleotides was found in preparations from systemically infected leaves of PD(-) transcriptand sap-inoculated plants. CNV coat protein could not be detected by ELISA or ISEM in PD(-)-infected leaf material. The ca. 3.8 kb RNA, when cloned and sequenced, was found to have lost all but 74 of the 1140 nucleotides of the CNV coat protein open reading frame.
Antilles Arc: a Bayesian Approach

18 The Lesser Antilles subduction zone is a challenging region when it comes to unravel19 ing its seismogenic behavior. Over the last century, the subduction megathrust has been 20 seismically quiet, with no large thrust event recorded, which raises the question whether 21 this subduction zone is able to produce large interplate earthquakes or not. However, 22 two historical earthquakes in the 19th century, a M 7-8 in 1839 and M 7.5-8.5 in 1843, 23 are proposed to have occurred along the subduction megathrust, although no direct ev24 idence exists. Here we provide a new assessment of interseismic coupling for the Lesser 25 Antilles subduction zone, based on updated GPS velocities and the latest models of the 26 slab geometry and elastic crustal structure. We use a Bayesian approach, allowing us to 27 explore the entire range of plausible models and to provide realistic estimates of inter28 seismic coupling and associated uncertainties. We find low to very low coupling along 29 the entire plate interface, including in the proposed rupture areas of the 1839 and 1843 30 events, where the sensitivity of our model is high. While a further understanding of tem31 poral variations in interseismic coupling needs to be addressed in future studies, our re32 sults indicate that the Lesser Antilles subduction zone is uncoupled, which challenges 33 the idea that the 1839 and 1843 earthquakes were thrust events. The updated GPS ve34 locities of this work now also reveal a small, but detectable amount of along-arc exten35 sion, consistent with geological observations of active normal faulting within the arc. 36 Plain Language Summary 37 The Lesser Antilles subduction zone forms the boundary between the Northand 38 South American plates that sink underneath the overlying Caribbean plate. Such down39 going movement typically results in the buildup of stress along the frictional interface 40 between the plates. When these stresses overcome the strength of the plate interface, they 41 can be released through earthquakes, that may have devastating effects on societies. By 42 using measurements from GPS stations on the islands of the Lesser Antilles, we aim to 43 determine how much strain is currently being accumulated along the subduction inter44 face, that is, how coupled the interface is. A high degree of coupling means that large 45 “megathrust” earthquakes are likely, while a low coupling means that they are less likely 46 and/or very rare. Two large earthquakes struck the Lesser Antilles in the 19th century, 47 which have been interpreted to have occurred on the subduction interface. In this work 48 we find a low to very low coupling along the plate interface, which implies that (1) these 49 historical earthquakes are unlikely to have occurred on the subduction interface but rather 50 deeper within the downgoing plate, and (2) large, Tohoku-like, megathrust earthquakes 51 are unlikely and/or must be very rare in the Lesser Antilles. 52
The Chloroplast rpl2 Intron and ORF184 as Phylogenetic Markers in the Legume Tribe Desmodieae

A previous extensive survey showed that most but not all members of the papilionoid tribe Desmodieae lack the intron for the chloroplast gene rpl2 and partially or completely lack the open reading frame 0RF184. We extended the survey of Desmodieae to include all 26 genera recognized in the most recent comprehensive taxonomic treatment of the tribe, and use slot blot hybridization with gene- or intron-specific probes for all taxa, polymerase chain reaction amplification across the rpl2 intron region for a subset of taxa, and DNA sequencing across the rpl2 intron/exon boundary in representatives of selected genera (Desmodium, Lepesdeza, and Phylacium). Our results indicate that rpl2 intron is absent from both genera of the largest subtribe, Desmodiinae, and from core Lespedezinae (Campylotropis, Kummerowia, and Lespedeza). It is indi- cated as present in both genera of Bryinae and in two Lespedezinae genera (Neocollettia and Phylacium). In contrast to the rpl2 intron, the pattern of 0RF184 loss in Desmodiinae and Lespedezinae was extremely complex and difficult to interpret from slot blots, but consistent either with partial deletion or extensive sequence divergence. Bryinae showed no such evidence of loss or partial deletion. In agreement with the distribution of the rpl2 intron, Bryinae have been considered to have affinities outside Desmodieae, while both Phylacium and Neocollettia are anomalous within Lespedezinae. Thus the rpl2 intron loss may represent a synapomorphy for a "core" Desmodieae (Desmodiinae and Lespedezinae).
STUDY OF WEATHER PREDICTION USING BAROTROPIC VORTICITY MODEL

Numerical weather prediction (NWP) plays significant role on daily human life, agriculture, economy and disaster warning. The main aim of this study is to predict (24, 48, 72 and 96) hours ahead of forecast mid-latitude (500) hpa geopotential height by solution of barotropic vorticity equation. Inputted wind velocity was obtained from (ECMWF) for (500) hpa pressure level on one selected day for both winter and summer. The result shows that the model have good accuracy in predict low and high systems but there is a little shifting in determining systems location, And the model present same geopotential height gradient with pattern but it has a shifting from actual pattern. Also The model sensitivity test are carried out, which shows that efficient prediction is below horizontal wind value of (60 m/h), while vice versa for high wind lead to loss of predictability.
CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication

Abstract ATR functions as a master regulator of the DNA-damage response. ATR activation requires the ATR activator, topoisomerase IIβ-binding protein 1 (TopBP1). However, the underlying mechanism of TopBP1 regulation and how its regulation affects DNA replication remain unknown. Here, we report a specific interaction between TopBP1 and the histone demethylase PHF8. The TopBP1/PHF8 interaction is mediated by the BRCT 7+8 domain of TopBP1 and phosphorylation of PHF8 at Ser854. This interaction is cell-cycle regulated and phosphorylation-dependent. PHF8 is phosphorylated by CK2, which regulates binding of PHF8 to TopBP1. Importantly, PHF8 regulates TopBP1 protein level by preventing its ubiquitination and degradation mediated by the E3 ligase UBR5. Interestingly, PHF8pS854 is likely to contribute to regulation of TopBP1 stability and DNA replication checkpoint. Further, both TopBP1 and PHF8 are required for efficient replication fork restart. Together, these data identify PHF8 as a TopBP1-binding protein and provide mechanistic insight into how PHF8 regulates TopBP1 stability to maintain DNA replication.
Biological activities of a recombinant fortilin from Fenneropenaeus merguiensis

Human Fortilin, an antiapoptotic protein, has also been implicated in several diseases; however, several potential uses of fortilin have also been proposed. Bearing the implications of fortilin in mind, fortilin analog, which has no complication with diseases, is required. Since a recombinant full-length fortilin from Fenneropenaeus merguiensis (rFm-Fortilin (FL)) reported only 44% (3e-27) homologous to human fortilin, therefore the biological activities of the Fm-Fortilin (FL) and its fragments (F2, F12, and F23) were investigated for potential use against HEMA toxicity from filling cement to pulp cell. The rFm-Fortilin FL, F2, 12, and F23 were expressed and assayed for proliferation activity. The rFm-Fortilin (FL) showed proliferation activity on human dental pulp cells (HDPCs) and protected the cells from 2-hydroxy-ethyl methacrylate (HEMA) at 1–20 ng/ml. In contrast, none of the rFm-Fortilin fragments promoted HDPC growth that may be due to a lack of three conserved amino acid residues together for binding with the surface of Rab GTPase for proliferative activity. In addition, rFm-Fortilin (FL) activated mineralization and trend to suppressed production of proinflammatory cytokines, including histamine (at 10 ng/ml) and TNF-α (at 100 ng/ml). Besides, the rFm-Fortilin (FL) did not mutate the Chinese hamster ovary (CHO) cell. Therefore, the rFm-Fortilin (FL) has the potential use as a supplementary medical material to promote cell proliferation in patients suffering severe tooth decay and other conditions.
Low Pathogenic Strain of Infectious Pancreatic Necrosis Virus (IPNV) Associated with Recent Outbreaks in Iranian Trout Farms

Infectious pancreatic necrosis (IPN), first described as acute viral catarrhal enteritis, is a highly contagious disease with variable pathogenicity that has been linked to genetic variation in the viral VP2 gene encoding the capsid protein. In this study, the IPN virus (IPNV) is isolated from the moribund fish from five of fourteen Iranian trout farms from 2015 to 2017. The affected fish showed mortality rates ranging from 20% to 60%, with the main clinical signs of exophthalmia, darkened skin, and mild abdominal distension, as well as yellow mucoid fluid in the intestine. Histopathological examination of intestinal sections confirmed acute catarrhal enteritis in all samples. RT-PCR assay of the kidney tissue and cell culture (CHSE-214) samples consistently confirmed the presence of the virus. The phylogenetic analysis of the partial VP2 sequence revealed that the detected isolates belong to genogroup 5, and are closely related to the Sp serotype strains of European origin. Characterization of VP2 of all isolates revealed the P217T221 motif that previously was associated with avirulence or low virulence, while all IPNV-positive fish in this study were clinically affected with moderate mortality. The IPNV isolates from Iran are associated with two lineages that appear to have originated from Europe, possibly via imported eggs.
Preparation of porous Co-Pt alloys for catalytic synthesis of carbon nanofibers

A simple and convenient procedure for the production of highly dispersed porous Co-Pt alloys to be used as catalysts for the synthesis of nanostructured carbon fibers (CNF) has been developed. The technique is based on the thermal decomposition of specially synthesized multicomponent precursors in a reducing atmosphere. A series of porous single-phase alloys Co-Pt (10–75 at% Pt) have been synthesized. The alloys containing 75 and 50 at% Pt were identified by the x-ray diffraction analysis as the intermetallics CoPt3 and CoPt, respectively. Within the region of 10–35 at% Pt, the synthesized alloys are represented by Co1−x Pt x random solid solutions with face-centered cubic lattice. The alloys obtained are characterized by a porous structure consisting of assembled fragments with a size of 50–150 nm. The obtained alloys were tested in the catalytic chemical vapor deposition of the ethylene to CNF. A significant synergistic effect between Co and Pt in the synthesis of carbon nanomaterials (CNMs) was revealed. The yield of CNF (for 30 min reaction) for catalysts containing 25–35 at% Pt was 30–38 g(CNF)/g(cat), whereas those for Co (100%) and Pt (100%) samples were as low as 5.6 and >0.1 g(CNF)/g(cat), respectively. The produced CNM composed of fibers with a segmented structure was shown to be characterized by a rather high specific surface area (200–250 m2 g−1) and structural homogeneity.
Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

ABSTRACT The colonic mucus layer, comprised of highly O-glycosylated mucins, is vital to mediating host-gut microbiota interactions, yet the impact of dietary changes on colonic mucin O-glycosylation and its associations with the gut microbiota remains unexplored. Here, we used an array of omics techniques including glycomics to examine the effect of dietary fiber consumption on the gut microbiota, colonic mucin O-glycosylation and host physiology of high-fat diet-fed C57BL/6J mice. The high-fat diet group had significantly impaired glucose tolerance and altered liver proteome, gut microbiota composition, and short-chain fatty acid production compared to normal chow diet group. While dietary fiber inclusion did not reverse all high fat-induced modifications, it resulted in specific changes, including an increase in the relative abundance of bacterial families with known fiber digesters and a higher propionate concentration. Conversely, colonic mucin O-glycosylation remained similar between the normal chow and high-fat diet groups, while dietary fiber intervention resulted in major alterations in O-glycosylation. Correlation network analysis revealed previously undescribed associations between specific bacteria and mucin glycan structures. For example, the relative abundance of the bacterium Parabacteroides distasonis positively correlated with glycan structures containing one terminal fucose and correlated negatively with glycans containing two terminal fucose residues or with both an N-acetylneuraminic acid and a sulfate residue. This is the first comprehensive report of the impact of dietary fiber on the colonic mucin O-glycosylation and associations of these mucosal glycans with specific gut bacteria.
A SURVEY OF THE PARASITES OF FIVE SPECIES OF BATS1'2

The ectoparasites and endoparasites of Mormoops megalophylla, Myotis velifer, Eptesicus fuscus, Corynorhinus rafinesquei, and Tadarida brasiliensis were collected, identified, and counted. The ectoparasites included fleas, streblids, nycteribiids, mites, and ticks. Mites were the only external parasites found on all five bat species. Endoparasites included trematodes, cestodes, and nematodes. No effort was made to find blood parasites.
Factors for Conversion of Fungal Biovolume Values to Biomass, Carbon and Nitrogen: Variation with Mycelial Ages, Growth Conditions, and Strains of Fungi from a Salt Marsh

Direct-count analysis of fungal biomass cannot be accurate or precise without firm knowledge of values for quantity of mass per unit volume of mycelium. We have determined a range in weight of dry matter, carbon and nitrogen per cm3 of fresh mycelium for salt-marsh fungi, using microecosystems which were rough simulations of a range of natural environments of fungi in salt marshes dominated by Spartina alterniflora Loisel. We found a wide range in dry matter per cm3 of mycelium, averaging 0.2 g cm3 for a group of less dense samples, and 0.9 g cm3 for more dense samples. The high densities were associated with young mycelium of two species known to be well adapted to the salt-marsh environment. The low densities were found for old mycelium, and for young and old mycelium of a species whose degree of adaptation to the salt-marsh environment is uncertain. Nitrogen and carbon percentages of dry fungal biomass were similar to those reported by other investigators (nitrogen, -4%; carbon, -35%).
Expansion and Refinement of Deep Sequence-Coupled Biopanning Technology for Epitope-Specific Antibody Responses in Human Serum

Identifying the specific epitopes targeted by antibodies elicited in response to infectious diseases is important for developing vaccines and diagnostics. However, techniques for broadly exploring the specificity of antibodies in a rapid manner are lacking, limiting our ability to quickly respond to emerging viruses. We previously reported a technology that couples deep sequencing technology with a bacteriophage MS2 virus-like particle (VLP) peptide display platform for identifying pathogen-specific antibody responses. Here, we describe refinements that expand the number of patient samples that can be processed at one time, increasing the utility of this technology for rapidly responding to emerging infectious diseases. We used dengue virus (DENV) as a model system since much is already known about the antibody response. Sera from primary DENV-infected patients (n = 28) were used to pan an MS2 bacteriophage VLP library displaying all possible 10-amino-acid peptides from the DENV polypeptide. Selected VLPs were identified by deep sequencing and further investigated by enzyme-linked immunosorbent assay. We identified previously described immunodominant regions of envelope and nonstructural protein-1, as well as a number of other epitopes. Our refinement of the deep sequence-coupled biopanning technology expands the utility of this approach for rapidly investigating the specificity of antibody responses to infectious diseases.
Age, growth and food of Cheimerius nufar (Ehrenberg, 1820) (Sparidae), collected off St Croix Island, Algoa Bay

Age and growth of the santer, Cheimerius nufar, were determined from otoliths. One hyaline and one opaque ring are laid down yearly. The von Bertalanffy growth function yielded the equation Lt = 953,56 [1-e-654(t + 2,6177)]. The length-mass relationship W = 0,00005 L2,7831 was used to determine growth in mass: Wt = 9790,24 [1-e-0,0654(t+2,6177)]2,7831. Stomach and intestine content analyses were done, using a modification of the points method, as well as the frequency of occurrence method. C. nufar is primarily a piscivore, but also preys on cephalopods and crustaceans.