Miracle-dz/UniFAR-FTUs huggingface dataset:
Aluminum-Induced Alterations to the Cell Wall and Antioxidant Enzymes Involved in the Regulation of the Aluminum Tolerance of Chinese Fir (Cunninghamia lanceolata)

Chinese fir (Cunninghamia lanceolata), which is an important coniferous tree species in China, is mainly planted in acidic soils with toxic aluminum (Al) levels. However, the consequences of Al toxicity and its resistance mechanism in Chinese fir remain largely uncharacterized. In this study, the Al-induced modification and possible role of cell wall in regulating Al tolerance in Chinese fir were investigated by using seedlings with contrasting Al tolerance, namely, Al-sensitive (YX02) and Al-resistant (YX01) genotypes. The results in present work showed that Al treatment resulted in a dose- and time-dependent inhibition of root growth and oxidative damage in both genotypes, but more in YX02 than in YX01. The severe oxidative damage observed in YX02 under Al stress was found to correlate with lower antioxidant enzyme activities as compared with YX01. The greater root growth inhibition observed in YX02 compared with YX01 was associated with a higher accumulation of Al in pectin and hemicllulose 1 (HC1) fraction because of the higher pectin and HC1 contents and the lower degree of pectin demethylation due to enhanced pectin methylesterase activity in YX02, which ultimately enhanced cell wall binding capacity for Al in YX02. Taken together, our results suggested that enhancement of antioxidant enzyme activities and cell wall modification-induced Al exclusion are the two mechanisms responsible for the Al tolerance of Chinese fir.
TRP Channels as Molecular Targets to Relieve Endocrine-Related Diseases

Transient receptor potential (TRP) channels are polymodal channels capable of sensing environmental stimuli, which are widely expressed on the plasma membrane of cells and play an essential role in the physiological or pathological processes of cells as sensors. TRPs often form functional homo- or heterotetramers that act as cation channels to flow Na+ and Ca2+, change membrane potential and [Ca2+]i (cytosolic [Ca2+]), and change protein expression levels, channel attributes, and regulatory factors. Under normal circumstances, various TRP channels respond to intracellular and extracellular stimuli such as temperature, pH, osmotic pressure, chemicals, cytokines, and cell damage and depletion of Ca2+ reserves. As cation transport channels and physical and chemical stimulation receptors, TRPs play an important role in regulating secretion, interfering with cell proliferation, and affecting neural activity in these glands and their adenocarcinoma cells. Many studies have proved that TRPs are widely distributed in the pancreas, adrenal gland, and other glands. This article reviews the specific regulatory mechanisms of various TRP channels in some common glands (pancreas, salivary gland, lacrimal gland, adrenal gland, mammary gland, gallbladder, and sweat gland).
Improved Utilization of Nitrate Nitrogen Through Within-Leaf Nitrogen Allocation Trade-Offs in Leymus chinensis

The Sharply increasing atmospheric nitrogen (N) deposition may substantially impact the N availability and photosynthetic capacity of terrestrial plants. Determining the trade-off relationship between within-leaf N sources and allocation is therefore critical for understanding the photosynthetic response to nitrogen deposition in grassland ecosystems. We conducted field experiments to examine the effects of inorganic nitrogen addition (sole NH4+, sole NO3– and mixed NH4+/NO3–: 50%/50%) on N assimilation and allocation by Leymus chinensis. The leaf N allocated to the photosynthetic apparatus (NPSN) and chlorophyll content per unit area (Chlarea) were significantly positively correlated with the photosynthetic N-use efficiency (PNUE). The sole NO3– treatment significantly increased the plant leaf PNUE and biomass by increasing the photosynthetic N allocation and Chlarea. Under the NO3 treatment, L. chinensis plants devoted more N to their bioenergetics and light-harvesting systems to increase electron transfer. Plants reduced the cell wall N allocation or increased their soluble protein concentrations to balance growth and defense under the NO3 treatment. In the sole NH4+ treatment, however, plants decreased their N allocation to photosynthetic components, but increased their N allocation to the cell wall and elsewhere. Our findings demonstrated that within-leaf N allocation optimization is a key adaptive mechanism by which plants maximize their PNUE and biomass under predicted future global changes.
The Linkage Between Leadership Styles, Employee Loyalty, and Turnover Intention in Healthcare Industry

The coronavirus disease-2019 (COVID-19) pandemic in Indonesia has resulted in a heavy burden upon the country’s healthcare system, especially frontline health workers who are at constant risk of exposure to COVID-19 (Fahlevi and Alharbi, 2021a). Nearly two thousand health workers have died during the COVID-19 outbreak (BBC, 2021), and the death rate for health workers in Indonesia is the highest in Asia and the third-largest in the world. Apart from the risk of infection, another serious risk is the effect on workers’ mental health, including burnout syndrome and mental fatigue (Vizheh et al., 2020). Health workers traditionally have high exposure to very tremendous levels of stress, but no rules or policies currently exist that adequately protect them. Research conducted by a research team from the Master of Occupational Medicine Study Program, Faculty of Medicine, University of Indonesia (2020) determined that as many as 83% of health workers in Indonesia have experienced moderate and severe burnout syndrome, a statistic which threatens the effective operation of the healthcare system as a whole. The high risk of healthcare workers suffering from burnout syndrome due to increased exposure to unusually severe stress during the pandemic can have long-term effects on the quality of medical services. Workers can feel depressed, experience extreme fatigue, and feel a lack of competence in carrying out their duties, all of which have a negative impact on efforts to fight COVID19 (Chirico et al., 2020). The Research Team from the Master of Occupational Medicine Study Program, consisting of Dr. Ray W Basrowi, MKK; Dr. Levina Chandra Khoe, MPH; and Dr. Marsen Isbayuputra, SpOK, found that in addition to the percentage of workers experiencing burnout syndrome (Faculty of Medicine, University of Indonesia, 2020):
Evaluation of Ecological Suitability and Quality Suitability of Panax notoginseng Under Multi-Regionalization Modeling Theory

Panax notoginseng is an important medicinal plant in China, but there are some limitations in the ecological suitability study, such as incomplete investigation of species distribution, single regionalization modeling, and lack of collaborative evaluation of ecological suitability, and quality suitability. In this study, the maximum entropy model was used to analyze the ecological suitability of P. notoginseng under current and future climates. The multi-source chemical information of samples was collected to evaluate the uniformity between quality and ecology. The results showed that the current suitable habitat was mainly in southwest China. In the future climate scenarios, the high suitable habitat will be severely degraded. Modeling based on different regionalization could predict larger suitable habitat areas. The samples in the high suitable habitat had both quality suitability and ecological suitability, and the accumulation of chemical components had different responses to different environmental factors. Two-dimensional correlation spectroscopy combined with deep learning could achieve rapid identification of samples from different suitable habitats. In conclusion, global warming is not conducive to the distribution and spread of P. notoginseng. The high suitable habitat was conducive to the cultivation of high-quality medicinal materials. Actual regionalization modeling had more guiding significance for the selection of suitable habitats in a small area. The multi-regionalization modeling theory proposed in this study could provide a new perspective for the ecological suitability study of similar medicinal plants. The results provided a reference for the introduction and cultivation, and lay the foundation for the scientific and standardized production of high-quality P. notoginseng.
Considerations for Balance Between Fundamental Treatment and Improvement of Quality of Life of Pediatric Thyroid Cancer Patient: Comparative Analysis With Adult Using Propensity Score Matching

Background Thyroid cancer is very rarely observed in children and adolescents, some reports have shown that the long-term outcome of treatment is better than that of adult patients, despite many treatment failures or a high risk of recurrence. This study considers whether it is appropriate to treat pediatric thyroid cancer patients aggressively, as per the ATA guidelines, based on the balance between the fundamental treatment of thyroid cancer and the improvement of the long-term quality of life of pediatric patients. Methods A total of 1,950 patients were recruited, including 83 pediatric and 1,867 adult patients, who were diagnosed with thyroid cancer and underwent surgical treatment at one of our medical center hospitals from March 2000 to January 2020. Results Sixty-nine pairs of pediatric and adult patients were matched in a ratio of 1:2 through propensity score matching. When compared through propensity score matching, there was no significant difference in prognosis such as recurrence rate in children and adults at the same stage. Conclusion This study showed that the prognosis of both pediatric and adult patients who underwent a total thyroidectomy and lobectomy was not significantly different. If more pediatric patients can be considered for the less-aggressive lobectomy than a total thyroidectomy through various preoperative examinations and meticulous pre-diagnosis, it may be possible to properly determine the balance between improving long-term quality of life while providing fundamental cancer treatment.
Diagnostic Value of Delayed Contrast-Enhanced Cardiac Computed Tomography for Detecting Left Atrial Appendage Thrombus in Patients With Atrial Fibrillation

Objective Delayed enhancement cardiac CT is a reliable tool for the diagnosis of left atrial appendage thrombus but limited for scanning heterogeneity. We aimed to explore the improvement of the 1 and 3-min delay phase at the diagnostic level to detect left atrial appendage thrombus, in order to set up a reasonable CT scanning scheme. Materials and Methods A total of 6,524 patients were continuously retrieved from January 2015 to September 2020 retrospectively. The patients had undergone Transesophageal echocardiography (TEE) and cardiac CT with complete period include the arterial enhancement phase, 1 and 3-min delay phase, TEE were used as the reference standard. The final study included 329 patients. Three experienced radiologists independently assessed each phase of the cardiac CT images for thrombus diagnosis. We explored the improvement of the diagnostic ability of different delayed contrast-enhanced phases for left atrial appendage thrombus detection. Multiple logistic regression analysis were used for further high-risk stratification to avoid an additional 1-min delayed scan. Results In total, 29 thrombosis were detected at TEE. For all cardiac CT phases, sensitivity and negative predictive were 100%. The specificity were 0.54, 0.93, and 1.00, respectively; The positive predictive values (PPV) were 0.17, 0.57, and 1.00, respectively; Area under curve (AUC) were 0.75, 0.95, and 0.98, respectively. High risk factors that cannot be clearly diagnosed with 1-min delay phase included reduced cardiac function, increased CHA2DS2-VAScscore and left atrial enlargement. Compared with the arterial enhanced phase, increased radiation doses in the 1 and 3-min delay phases were 1.7 ± 1.3 msv and 1.5 ± 0.8 msv (mean ± standard deviation). Conclusion Using TEE as the reference standard, early contrast-enhanced CT scanning with 1 and 3-min delay is necessary for the diagnosis of left appendage thrombus, which could significantly improve the diagnostic efficiency. Patients with high-risk stratification are suitable for direct 3-min delayed scanning.
Dietary Energy and Protein Levels During the Prelay Period on Production Performance, Egg Quality, Expression of Genes in Hypothalamus-Pituitary-Ovary Axis, and Bone Parameters in Aged Laying Hens

Nutrition during the pre-lay period takes effect on the production performance in the laying flock. This study evaluated the effects of dietary energy and protein levels in pre-lay diet on performance during the whole laying period and the egg quality, bone quality, and mRNA expression of hypothalamus-pituitary-gonadal (HPG) axis-related genes of hens at the end of the laying cycle. A total of 1,856 15-wk old Hy-Line brown pullets were randomly assigned to one of the four dietary treatments: using a 2 × 2 factorial arrangement with 2 energy levels (2,700 and 2,800 kcal/kg ME, respectively) and 2 protein levels (15 and 16.5% CP, respectively). Pullets were fed ad libitum from 15 to 20 wk and from 20 wk onward, fed with a similar laying diet till 72 wk of age. At 72 wk, the expression of genes in the hypothalamus, pituitary, ovarian, and follicles and bone quality was evaluated. At 72wk, there were no differences in production performance, BW, organ index, and ovarian parameters among the dietary treatments. High-CP diet increased the egg shape index and eggshell thickness (p < 0.05), but the eggshell breaking strength, Haugh unit, and albumen height did not differ among the treatments. Neither dietary energy nor protein level took an effect of bone quality. Low-energy diet increased the mRNA expression of gonadotropin-releasing hormone-1 (GnRH-1) in the hypothalamus (p < 0.05). The mRNA expression level of estrogen receptor-1 (ESR-1) in the hypothalamus and ovary was elevated by the 2,700 ME-15%CP diet (p < 0.05). The expression of cytochrome family 17 subfamily A polypeptide 1 (CYP17A1) in the large white follicle (LWF), small yellow follicles (SYF) and dominant follicle (DF) was decreased by the 2,800 kcal/kg diet (p < 0.05). These results indicate that the prelay diet had no influence on the production performance but had minimal effect on the eggshell characteristics and bone parameters. These results suggest that the energy and protein level of the prelay diet changes the expression of HPG axis-related genes of hens around the end of the laying cycle without changing the circulating sex hormone profile. The effect of prelay diet on the endocrinal adjustment at the end of the laying cycle needs to be investigated further.
Molecular Mechanism of the β-Lactamase Mediated β-Lactam Antibiotic Resistance of Pseudomonas aeruginosa Isolated From a Chinese Teaching Hospital

Pseudomonas aeruginosa can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. To investigate the molecular characteristics of β-lactam antibiotic resistance of P. aeruginosa isolated from a hospital population between 2015 and 2017, in this study, the antimicrobial susceptibility and the resistance gene profile of the bacteria were determined. The Pulsed-field gel electrophoresis (PFGE) was used to characterize the clonal relatedness and sequencing and comparative genomic analysis were performed to analyze the structure of the resistance gene-related sequences. As a result, of the 260 P. aeruginosa strains analyzed, the resistance rates for 6 β-lactam antibiotics ranged from 4.6 to 9.6%. A total of 7 genotypes of 44 β-lactamase genes were identified in 23 isolates (8.9%, 23/260). Four transconjugants from different donors carrying blaCARB-3 exhibited a phenotype of reduced susceptibility to piperacillin–tazobactam, ceftazidime, and cefepime, and 2 transconjugants harboring blaIMP-45 exhibited a phenotype of reduced susceptibility to carbapenems. blaCARB positive isolates (n = 12) presented six PFGE patterns, designated groups A to F. Two bla genes (blaIMP-45 and blaOXA-1) in PA1609 related to a class 1 integron (intI1-blaIMP-45-blaOXA-1-aac(6′)-Ib7-catB3-qacE∆1-sul1) were encoded on a plasmid (pPA1609-475), while the blaCARB-3 gene of PA1616 also related to a class 1 integron was located on the chromosome. The results suggest that β-lactam antibiotic resistance and clonal dissemination exist in this hospital population. It indicates the necessity for molecular surveillance in tracking β-lactamase-producing strains and emphasizes the need for epidemiological monitoring.
Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin

Microtubules are cytoskeletal filaments underlying the morphology and functions of all eukaryotic cells. In higher eukaryotes, the basic building blocks of these non-covalent polymers, ɑ- and β-tubulins, are encoded by expanded tubulin family genes (i.e., isotypes) at distinct loci in the genome. While ɑ/β-tubulin heterodimers have been isolated and examined for more than 50 years, how tubulin isotypes contribute to the microtubule organization and functions that support diverse cellular architectures remains a fundamental question. To address this knowledge gap, in vitro reconstitution of microtubules with purified ɑ/β-tubulin proteins has been employed for biochemical and biophysical characterization. These in vitro assays have provided mechanistic insights into the regulation of microtubule dynamics, stability, and interactions with other associated proteins. Here we survey the evolving strategies of generating purified ɑ/β-tubulin heterodimers and highlight the advances in tubulin protein biochemistry that shed light on the roles of tubulin isotypes in determining microtubule structures and properties.
Exploring English as a Foreign Language Instructors’ Self-Derived English Language Policies at Higher Education Level: A Case Study in the Saudi Context

Education in Saudi Arabia employs English as a foreign language. Several researchers have examined language policies governing English use. Current research suggests that official policies do not exist and self-English language policies (ELPs) are in place without any type of governance. Therefore, this study aims to investigate the nature of self-ELPs in Saudi Arabia and how the country can benefit from engendering institutional changes. An eight-item survey was conducted with an open-ended section and received responses from faculty members affiliated with English departments around the country (n = 213). Semi-structured interviews with eight chairpersons from different English departments were also organized. The findings reveal that the majority of faculty practice self-ELPs very frequently, in both official and non-official situations. It is concluded that English departments in the academia in Saudi Arabia are ready to enforce official ELPs and accept the idea of internationalizing their academic services worldwide.
Using a Design Science Research Methodology Process to Design a Text Entry Technique for Mobile VR

Mobile-based virtual reality (VR) is the more affordable way for the general public to experience immersive VR, and text input is a frequent process in mobile VR. Usually, the raycasting selection technique is used to perform this task. However, using this technique may present some limitations. Hence, this short paper aims to briefly present the work in progress of designing and developing an alternative text entry technique for mobile-based VR using a design science research (DSR) methodology process. Our technique uses a one-handed ambiguous keyboard and focuses on improving user performance and typing experience for short-term text input. Further improvement and the evaluation of the technique will compose the next steps of this research.
Fullerenes’ Interactions with Plasma Membranes: Insight from the MD Simulations

Understanding the interactions between carbon nanoparticles (CNPs) and biological membranes is critically important for applications of CNPs in biomedicine and toxicology. Due to the complexity and diversity of the systems, most molecular simulation studies have focused on the interactions of CNPs and single component bilayers. In this work, we performed coarse-grained molecular dynamic (CGMD) simulations to investigate the behaviors of fullerenes in the presence of multiple lipid components in the plasma membranes with varying fullerene concentrations. Our results reveal that fullerenes can spontaneously penetrate the plasma membrane. Interestingly, fullerenes prefer to locate themselves in the region of the highly unsaturated lipids that are enriched in the inner leaflet of the plasma membrane. This causes fullerene aggregation even at low concentrations. When increasing fullerene concentrations, the fullerene clusters grow, and budding may emerge at the inner leaflet of the plasma membrane. Our findings suggest by tuning the lipid composition, fullerenes can be loaded deeply inside the plasma membrane, which can be useful for designing drug carrier liposomes. Moreover, the mechanisms of how fullerenes perturb multicomponent cell membranes and how they directly enter the cell are proposed. These insights can help to determine fullerene toxicity in living cells.
Non-Binding Secret Reserve Prices: The Case of Wholesale Used-Car Auctions

I study secret reserve prices in auctions that are non-binding in the sense that the sellers can accept bids below them. Such a reserve price has a bite only when the winning bid exceeds it, in which case the winning bid is accepted without seller’s action. This work investigates the motivation for this puzzling practice that many real-world auctions take, such as wholesale used-car auctions. I estimate a structural model of ascending auctions using the auction data in the wholesale used-car market. To microfound seller’s decision of the secret reserve price, I posit that the seller has uncertainty as to the value of the item when she sets the reserve price and that this uncertainty is resolved after she observes the auction price. I compare the status quo with two counterfactual auction formats: (i) no reserve prices and the seller gets to accept or reject every winning bid, and (ii) the seller commits to the secret reserve price. I observe very little difference among them in terms of probability of trade, seller’s payoff and revenue. I discuss how the current format may be rationalized as reducing transaction costs for asking sellers’ conﬁrmation of all winning bids and avoiding sellers’ cognitive cost of committing to a reserve price. primitives — the CDF of private value component of sellers in particular can be estimated in an en-vironment of ascending auctions with non-binding secret reserve prices. I show that the probability that the seller accepts an auction price in the after-auction process is a convolution of the distribution of game-level heterogeneity (Comonent (ii)), the distribution of market hotness (Component (iii)), and the CDF of private quo non-binding secret non-binding secret reserve prices and binding secret reserve prices. similar after-auction