Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of an automatic threshold based detector of waveform limits in Holter ECG with the QT database

The authors evaluate a single-lead threshold based ECG wave boundaries detector with a QT database developed for validation purposes. They also identify its different sources of error distinguishing those that come from precision errors in boundary location from those that come from morphology misclassification. They obtain 71 % of records with correct morphology identification of T wave and variance in boundary location within manual referees variance. The remaining records analyzed correspond to signals with poor SNR at the T wave, or morphology discrepancies between algorithm and experts.
Action research as a congruent methodology for understanding wikis: the case of Wikiversity

It is proposed that action research is an appropriate methodology for studying wikis, and is akin to research 'the wiki way'. This proposal is contextualised within the case of Wikiversity, a project of the Wikimedia Foundation. A framework for a participative research project is outlined, and challenges and implications of such a methodology are discussed. Editors: Andreia Santos (Open University, UK). Reviewers: Rob Nadolski (Open University, NL), Ray Corrigan (Open University, UK), and Andreia Santos (Open University, UK).
The effect of green tea on inflammatory mediators: A systematic review and meta‐analysis of randomized clinical trials

Catechin in green tea might be able to reduce inflammatory mediators; therefore, in this study, we aimed to indicate green tea effects on inflammatory mediators such as tumor necrosis factor‐alpha (TNF‐α), C‐reactive protein (CRP), and interleukin‐6 (IL‐6). The advanced search methods of electronic databases were used to find randomized clinical trials that assessed green tea effect on inflammatory mediators among adult population. Google Scholar, PubMed/Medline, EMBASE, SCOPUS, and ISI Web of Science were searched until January 2019. Delphi checklist was used for assessing the quality of included articles. Mean changes in serum inflammatory biomarkers were calculated by subtracting endpoint values from the baseline in each study arm. Then the effect size for each selected study was estimated as the difference between mean changes in the intervention and control groups. We included 16 articles in our meta‐analysis and 17 articles in systematic review. Our results indicated that green tea could not significantly decrease serum CRP levels and significantly increased IL‐6 and significantly decreased TNF‐α levels. In conclusion, green tea might not be able to change inflammatory mediators especially in diseases with low inflammation, but scientists who want to assess green tea effect on inflammatory mediators should perform their study on patients with high inflammation. Studies exclusive on male or female and considering nutrients intake as a confounding factor are a necessity.
Molecular cloning, characterization and expression analysis of MHCI and chemokines CXCR3 and CXCR4 gene from freshwater carp, Catla catla

The immune system with large number of molecules protects the host against a plethora of continuously evolving microbes. Major histocompatibility complex (MHC) molecules serve as cardinal elements of the adaptive immune system responsible for the activation of the adaptive immunity in the host. The present study reports MHCI molecule in freshwater carp, Catla catla, and its differential expression in immunologically relevant tissues post‐infection with Gram‐negative and Gram‐positive bacteria. The MHCI sequence of C. catla had 502 bp nucleotides encoding putative 146 amino acids. The phylogenetic analysis exhibited its evolutionary conservation within the Cyprinidae family and formed a different clade with the higher vertebrates. Simultaneously, CXCR3 and CXCR4 chemokines were cloned and characterized for their expression in infected tissues. Analysis of immunologically relevant tissues of the infected fish exhibited an increase of MHCI gene expression and the down‐regulation of CXCR3 and CXCR4 chemokines, indicating a tricky interaction between the innate and adaptive immune system. It was found that intestine, skin and spleen played a crucial role in the contribution of the defense activity which instigated the self‐immunity. These immune activities can provide useful information to understand the interaction of self and non‐self‐ immune system in freshwater fish, Catla catla.
Rehabilitation of the Blind using Audio to Visual Conversion Tool

In human beings, eyes play a vital role. A very less research has been done for rehabilitation of blindness for the blind people. This paper discusses the work that helps blind people for recognizing the basic shapes of the objects like circle, square, triangle, horizontal lines, vertical lines, diagonal lines and the wave forms like sinusoidal, square, triangular etc. This is largely achieved by using digital camera, which is used to capture the visual information present in front of the blind person and a software program, which achieves the image processing operations, and finally the processed image is converted into sound. After the sound generation process, the generated sound is fed to the blind person through headphones for visualizing the imaginary image of the object. For visualizing the imaginary image of the object, it needs to train the blind person. Various training process methods had been applied for recognizing the object.
Cancer cachexia induces morphological and inflammatory changes in the intestinal mucosa

Cachexia is a multifactorial and multiorgan syndrome associated with cancer and other chronic diseases and characterized by severe involuntary body weight loss, disrupted metabolism, inflammation, anorexia, fatigue, and diminished quality of life. This syndrome affects around 50% of patients with colon cancer and is directly responsible for the death of at least 20% of all cancer patients. Systemic inflammation has been recently proposed to underline most of cachexia‐related symptoms. Nevertheless, the exact mechanisms leading to the initiation of systemic inflammation have not yet been unveiled, as patients bearing the same tumour and disease stage may or may not present cachexia. We hypothesize a role for gut barrier disruption, which may elicit persistent immune activation in the host. To address this hypothesis, we analysed the healthy colon tissue, adjacent to the tumour.
Extracting depth information from stereo linear images using a genetic approach

Stereo vision is one of the most important topics in computer vision. The goal is to compute depth information of a scene seen by two or more video cameras from different viewpoints. The key problem consists of identifying features in stereo images that are generated by the same physical feature in the three-dimensional space. In this paper we present a genetic approach to the stereo correspondence problem where a new solution encoding is proposed. To evaluate a solution, the fitness function is defined from three competing constraints, such that best matches correspond to its minima. Experimental results are presented to demonstrate the effectiveness of the proposed approach for localizing moving objects of a scene seen by a linear stereoscopic sensor.
The Contribution of a Charitable Organization to Regional Cleft Lip and Palate Services in England and Scotland

Background: From diagnosis through to adulthood, a cleft lip and/or palate (CL/P) poses a number of challenges for those affected. Alongside the care provided by clinical teams, complementary information and support is offered by charitable organizations. In 2011, the Cleft Lip and Palate Association received funding to implement a new regional service across England and Scotland, with the aim of increasing support at a local level. The Centre for Appearance Research at the University of the West of England were commissioned to conduct an independent evaluation of the service over 7 years. Methods: A pragmatic, mixed-methods approach was utilized to assess the impact of the service from the perspective of charity volunteers; children, young people, and adults with CL/P; caregivers; and clinicians. Feedback forms were distributed to stakeholders at a variety of events, and qualitative feedback was collected via focus groups and an online survey. Results: The majority of participants indicated they had gained access to a local support network, felt more able to cope with CL/P-related challenges, and felt more confident in themselves. Qualitative investigation provided further support for these findings and highlighted additional benefits of the regional service for clinical teams. Conclusions: The evaluation provides encouraging evidence toward the contribution of a relatively small charitable organization in the context of cleft care. The importance of a pragmatic approach to community-based evaluation and the benefits of collaborative working between researchers and the charitable sector were also highlighted.
Fault-tolerant QR decomposition for adaptive signal processing

We present an algorithm-based fault tolerant scheme for QR decomposition, that extends the well-known Gentleman-Kung-McWhirter triangular systolic array architecture. Assuming that the array is subject to transient faults, widely separated in time and each affecting a single processor, we give an algorithm that corrects the full triangular array with computational overhead equivalent, on average, to the interpolation of a single extra vector into the data stream.
Resonant frequency and radiation efficiency of meander line antennas

One of the approaches to reducing the size of half-wavelength linear dipole antennas is the meander dipole antenna, where the elements form a meander line. This paper presents a formula for the relationship between the geometrical size and the resonant frequency of the meander line dipole antenna, and a calculation formula for the radiative efficiency is derived from the result. It is shown that the geometrical parameters of the meander line dipole antenna can be determined from the specified radiative efficiency. © 1999 Scripta Technica, Electron Comm Jpn Pt 2, 83(1): 52–58, 2000
Visual Feedback Processing of the Limb Involves Two Distinct Phases

Muscle responses to mechanical disturbances exhibit two distinct phases: a response starting at ~20 ms that is fairly stereotyped, and a response starting at ~60 ms modulated by many behavioral contexts including goal-redundancy and environmental obstacles. Muscle responses to disturbances of visual feedback of the hand arise within ~90 ms. However, little is known whether these muscle responses are sensitive to behavioral contexts. We had 49 human participants (27 male) execute goal-directed reaches with visual feedback of their hand presented as a cursor. On random trials, the cursor jumped laterally to the reach direction, and thus, required a correction to attain the goal. The first experiment demonstrated that the response amplitude starting at 90 ms scaled with jump magnitude, but only for jumps <2 cm. For larger jumps, the duration of the muscle response scaled with the jump size starting after 120 ms. The second experiment demonstrated that the early response was sensitive to goal redundancy as wider targets evoked a smaller corrective response. The third experiment demonstrated that the early response did not consider the presence of obstacles, as this response routinely drove participants directly to the goal even though this path was blocked by an obstacle. Instead, the appropriate muscle response to navigate around the obstacle started after 120 ms. Our findings highlight that visual feedback of the limb involves two distinct phases: a response starting at 90 ms with limited sensitivity to jump magnitude and sensitive to goal-redundancy, and a response starting at 120 ms with increased sensitivity to jump magnitude and environmental factors. SIGNIFICANCE STATEMENT The motor system can integrate proprioceptive feedback to guide an ongoing action in ~60 ms and is flexible to a broad range of behavioral contexts. In contrast, the present study identified that the motor response to a visual disturbance exhibits two distinct phases: an early response starting at 90 ms with limited scaling with disturbance size and sensitivity to goal-redundancy, and a slower response starting after 120 ms with increased sensitivity to disturbance size and sensitive to environmental obstacles. These data suggest visual feedback of the hand is processed through two distinct feedback processes.
Emergence of Influenza A(H7N4) Virus, Cambodia

Active surveillance in high-risk sites in Cambodia has identified multiple low-pathogenicity influenza A(H7) viruses, mainly in ducks. None fall within the A/Anhui/1/2013(H7N9) lineage; however, some A(H7) viruses from 2018 show temporal and phylogenetic similarity to the H7N4 virus that caused a nonfatal infection in Jiangsu Province, China, in December 2017.
Attitudes toward advance care planning among persons with dementia and their caregivers

ABSTRACT Objectives: To examine factors that influence decision-making, preferences, and plans related to advance care planning (ACP) and end-of-life care among persons with dementia and their caregivers, and examine how these may differ by race. Design: Cross-sectional survey. Setting: 13 geographically dispersed Alzheimer’s Disease Centers across the United States. Participants: 431 racially diverse caregivers of persons with dementia. Measurements: Survey on “Care Planning for Individuals with Dementia. ” Results: The respondents were knowledgeable about dementia and hospice care, indicated the person with dementia would want comfort care at the end stage of illness, and reported high levels of both legal ACP (e.g., living will; 87%) and informal ACP discussions (79%) for the person with dementia. However, notable racial differences were present. Relative to white persons with dementia, African American persons with dementia were reported to have a lower preference for comfort care (81% vs. 58%) and lower rates of completion of legal ACP (89% vs. 73%). Racial differences in ACP and care preferences were also reflected in geographic differences. Additionally, African American study partners had a lower level of knowledge about dementia and reported a greater influence of religious/spiritual beliefs on the desired types of medical treatments. Notably, all respondents indicated that more information about the stages of dementia and end-of-life health care options would be helpful. Conclusions: Educational programs may be useful in reducing racial differences in attitudes towards ACP. These programs could focus on the clinical course of dementia and issues related to end-of-life care, including the importance of ACP.
Speaker adaptation through spectral transformation for HMM based speech recognition

The use of spectral transformation to perform speaker adaptation for HMM based speech recognition is investigated. Three estimation methods, namely, minimum mean square error (MMSE), canonical correlation analysis (CCA) and multilayer perceptrons (MLP), for computing the transformation are compared. Using isolated words from the TI-46 database, it is found that CCA has the best adaptation performance. Moreover, a "training-after-adaptation" approach is found to have a higher adaptation performance than the one in which reference HMMs are not re-trained. With a suitable choice of reference speaker, less than 30% of training data from a new speaker is required in order to achieve the same accuracy as the speaker-dependent models of that new speaker, when the CCA method is used with the "training-after-adaptation" approach. <<ETX>>
Self-Priming in Production: Evidence for a Hybrid Model of Syntactic Priming

Syntactic priming in language production is the increased likelihood of using a recently encountered syntactic structure. In this paper, we examine two theories of why speakers can be primed: error-driven learning accounts (Bock, Dell, Chang, & Onishi, 2007; Chang, Dell, & Bock, 2006) and activation-based accounts (Pickering & Branigan, 1999; Reitter, Keller, & Moore, 2011). Both theories predict that speakers should be primed by the syntactic choices of others, but only activation-based accounts predict that speakers should be able to prime themselves. Here we test whether speakers can be primed by their own productions in three behavioral experiments and find evidence of structural persistence following both comprehension and speakers' own productions. We also find that comprehension-based priming effects are larger for rarer syntactic structures than for more common ones, which is most consistent with error-driven accounts. Because neither error-driven accounts nor activation-based accounts fully explain the data, we propose a hybrid model.
Evolution of path finding

Based on evolutionary computation (EC) concepts, we developed an evolutionary navigator (EN) as an approach to path planning and navigation. The EN is characterized by generality, flexibility and adaptability. It integrates the planning process in the evolutionary algorithm, which: (1) accommodates different optimization criteria and changes in these criteria; (2) incorporates various types of problem specific domain knowledge; and (3) enables good tradeoffs among near-optimality of paths, high planning efficiency and effective handling of unknown obstacles.
Small-protein Enrichment Assay Enables the Rapid, Unbiased Analysis of Over 100 Low Abundance Factors from Human Plasma*

Small proteins in plasma regulate diverse processes from metabolism to immune functions and are difficult to study, because of the large dynamic range of protein abundance. To facilitate their detection, we developed a method using protein dissociation, precipitation and size exclusion chromatography called SPEA. Herein, we demonstrate SPEA is capable of reproducible characterization of known small proteins and identification of novel factors such as C5ORF46. When applied to study humans undergoing intermittent fasting, novel changes in iron regulation were observed. Graphical Abstract Highlights Development of a method for low-abundance small protein analysis in human plasma. Deep proteome profiling demonstrated unbiased identification of diverse factors. Method enabled identification of a novel human plasma protein C5ORF46. Analysis of intermittent fasting response showed changes in iron regulation. Unbiased and sensitive quantification of low abundance small proteins in human plasma (e.g. hormones, immune factors, metabolic regulators) remains an unmet need. These small protein factors are typically analyzed individually and using antibodies that can lack specificity. Mass spectrometry (MS)-based proteomics has the potential to address these problems, however the analysis of plasma by MS is plagued by the extremely large dynamic range of this body fluid, with protein abundances spanning at least 13 orders of magnitude. Here we describe an enrichment assay (SPEA), that greatly simplifies the plasma dynamic range problem by enriching small-proteins of 2–10 kDa, enabling the rapid, specific and sensitive quantification of >100 small-protein factors in a single untargeted LC-MS/MS acquisition. Applying this method to perform deep-proteome profiling of human plasma we identify C5ORF46 as a previously uncharacterized human plasma protein. We further demonstrate the reproducibility of our workflow for low abundance protein analysis using a stable-isotope labeled protein standard of insulin spiked into human plasma. SPEA provides the ability to study numerous important hormones in a single rapid assay, which we applied to study the intermittent fasting response and observed several unexpected changes including decreased plasma abundance of the iron homeostasis regulator hepcidin.
PRDM9 forms a trimer by interactions within the zinc finger array

PRDM9 forms a trimer as a soluble protein and in complex with DNA mediated by the ZnF domain. Five ZnFs are already sufficient for multimerization and only one DNA molecule is complexed in the trimer. PRDM9 is a trans-acting factor directing meiotic recombination to specific DNA-binding sites by its zinc finger (ZnF) array. It was suggested that PRDM9 is a multimer; however, we do not know the stoichiometry or the components inducing PRDM9 multimerization. In this work, we used in vitro binding studies and characterized with electrophoretic mobility shift assays, mass spectrometry, and fluorescence correlation spectroscopy the stoichiometry of the PRDM9 multimer of two different murine PRDM9 alleles carrying different tags and domains produced with different expression systems. Based on the migration distance of the PRDM9–DNA complex, we show that PRDM9 forms a trimer. Moreover, this stoichiometry is adapted already by the free, soluble protein with little exchange between protein monomers. The variable ZnF array of PRDM9 is sufficient for multimerization, and at least five ZnFs form already a functional trimer. Finally, we also show that only one ZnF array within the PRDM9 oligomer binds to the DNA, whereas the remaining two ZnF arrays likely maintain the trimer by ZnF–ZnF interactions.
Lack of the Major Multifunctional Catalase KatA in Pseudomonas aeruginosa Accelerates Evolution of Antibiotic Resistance in Ciprofloxacin-Treated Biofilms

During chronic biofilm infections, Pseudomonas aeruginosa bacteria are exposed to increased oxidative stress as a result of the inflammatory response. As reactive oxygen species (ROS) are mutagenic, the evolution of resistance to ciprofloxacin (CIP) in biofilms under oxidative stress conditions was investigated. ABSTRACT During chronic biofilm infections, Pseudomonas aeruginosa bacteria are exposed to increased oxidative stress as a result of the inflammatory response. As reactive oxygen species (ROS) are mutagenic, the evolution of resistance to ciprofloxacin (CIP) in biofilms under oxidative stress conditions was investigated. We experimentally evolved six replicate populations of P. aeruginosa lacking the major catalase KatA in colony biofilms and stationary-phase cultures for seven passages in the presence of subinhibitory levels (0.1 mg/liter) of CIP or without CIP (eight replicate lineages for controls) under aerobic conditions. In CIP-evolved biofilms, a larger CIP-resistant subpopulation was isolated in the ΔkatA strain than in the wild-type (WT) PAO1 population, suggesting oxidative stress as a promoter of the development of antibiotic resistance. A higher number of mutations identified by population sequencing were observed in evolved ΔkatA biofilm populations (CIP and control) than in WT PAO1 populations evolved under the same conditions. Genes involved in iron assimilation were found to be exclusively mutated in CIP-evolved ΔkatA biofilm populations, probably as a defense mechanism against ROS formation resulting from Fenton reactions. Furthermore, a hypermutable lineage due to mutL inactivation developed in one CIP-evolved ΔkatA biofilm lineage. In CIP-evolved biofilms of both the ΔkatA strain and WT PAO1, mutations in nfxB, the negative regulator of the MexCD-OprJ efflux pump, were observed while in CIP-evolved planktonic cultures of both the ΔkatA strain and WT PAO1, mutations in mexR and nalD, regulators of the MexAB-OprM efflux pump, were repeatedly found. In conclusion, these results emphasize the role of oxidative stress as an environmental factor that might increase the development of antibiotic resistance in in vivo biofilms.