Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of the cell wall and outer membrane of Rhodopseudomonas capsulata

Sucrose density gradient centrifugation of cell envelopes of chemotrophically grown cells of Rhodopseudomonas capsulata St. Louis (= ATCC 23782) resulted in the separation of a cytoplasmic membrane from a cell wall fraction (buoyant densities, 1.139 and 1.215 g/cm3, respectively). The cell wall fractions (untreated or Triton extracted) contained peptidoglycan- and lipopolysaccharide-specific components. Their neutral sugar content, mainly rhamnose and galactose, was high (250 and 100 micrograms/mg [dry weight] of material) due to a non-lipopolysaccharide polymer. The fatty acid content was low (less than or equal to 60 micrograms/mg [dry weight] of material), and half of it was contributed by lipopolysaccharide (3-OH-C10:0, C12:1, and 3-oxo-C14:0). The predominant other fatty acid was C18:1. An outer membrane fraction, obtained by lysozyme treatment of the Triton-extracted cell wall, showed essentially the same chemical composition except for almost complete removal of peptidoglycan. Saline extraction (0.9% NaCl, 37 degrees C, 2 h) removed a lipopolysaccharide-protein(-phospholipid? ) complex from whole cells of R. capsulata St. Louis. The polypeptide patterns of the cell wall and outer membrane as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis comprised 20 to 25 different polypeptides (most of them very faint) and were dominated by a single, heat-modifiable major protein (Mr 69,000 after solubilization below 60 degrees C; Mr 33,000 at temperatures above 70 degrees C).
Exercise and risk of injurious fall in home-dwelling elderly

OBJECTIVES: To examine the relationship between different types of physical exercise and the risk of subsequent fall-related injury. STUDY DESIGN: A prospective study of the home-dwelling elderly. METHODS: A population sample of home-dwelling subjects aged 85 years or older (n = 512) in northern Finland participated in the study. Baseline data were collected by home-nursing staff through postal questionnaires and clinical tests. Frequency and times of physical exercise—that is, walking exercise and other exercise (home exercise, group exercise, gardening, cross-country skiing, dancing, swimming, bicycling)–and falls were recorded by a nurse examiner, who telephoned the participants 8 times during a 2-year follow-up period. Statistical analyses were based on Cox regressions and pooled logistic regressions. RESULTS: The risk of injury-causing falls was reduced by other exercise taken at least 1 hour per week compared with corresponding non-exercise; adjusted odds ratio 0.37 (0.19–0.72) but not by walking exercise. The risk of injury-causing falls was not increased by any kind or amount of exercise taken. Female sex, a history of recent fall-related injury and poor baseline near-vision acuity were the other significant predictors of injury-causing falls. CONCLUSIONS: Habitual physical exercise proved to be safe and some of the exercises were associated with reduced risk of subsequent fall-related injury. Female sex, an injury-causing fall in the recent past and problems with near vision increased the risk.
High-resolution 31P nuclear magnetic resonance study of Chlamydia trachomatis: induction of ATPase activity in elementary bodies

ATPase activity of elementary bodies (EBs) of Chlamydia trachomatis was investigated by using high-resolution 31P nuclear magnetic resonance spectroscopy. ATPase activity was detected in EBs of C. trachomatis serovars A, B, and L2 after treatment with the reducing agents 2-mercaptoethanol and glutathione. ATPase activity was oligomycin sensitive and magnesium ion dependent. EBs heated at 60 degrees C for 10 min or pretreated with Triton X-100 before exposure to 2-mercaptoethanol did not exhibit ATPase activity. Monoclonal antibody to the major outer membrane protein abrogated ATPase activity of EBs, whereas monoclonal antibody to chlamydial lipopolysaccharide only marginally reduced the level of ATPase activity. These findings suggest that EBs possess intrinsic ATPase activity and that cysteine-rich outer membrane proteins of EBs are important in the regulation of ATPase activity. The major outer membrane protein may be the major route through which ATP accesses ATPase.
Downregulation of AFP Protein Expression by RNAi Inhibits the Growth of AFP-Producing Gastric Adenocarcinoma Cell Line

Many studies have examined the biological activities of Alpha-fetoprotein (AFP); however, the modulatory role of AFP in neoplastic growth remains unclear. Three hairpin siRNA expressing plasmids (pSIREN-DNR-DsRed-Express Donor-Vector-AFP-siRNAl/2/3) were constructed and transfected into FU97(AFP-producing gastric adenocarcinoma cell line). At 48h after transfection, the silencing efficiency to AFP gene expression was analyzed by RT-PCR and immunoassays. The influence to FU97 on its growth and apoptosis after silencing the AFP gene were observed by MTT and flow cytometry. The results of RT-PCR and immunoassays showed that the plasmids could inhibit AFP mRNA and protein expression at 48h after transfection. The pSIREN-DNR-DsRed-Express Donor Vector-AFP-siRNAl was more effective than other two. These plasmids could obviously inhibit FU97 cell growth and promote FU97 cell apoptosis.
Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons

Although spatially restricted Ca2+ release from the endoplasmic reticulum (ER) through intracellular Ca2+ channels plays important roles in various neuronal activities, the accurate distribution and dynamics of ER in the dendrite of living neurons still remain unknown. To elucidate these, we expressed fluorescent protein-tagged ER proteins in cultured mouse hippocampal neurons, and monitored their movements using time-lapse microscopy. We report here that a sub-compartment of ER forms in relatively large vesicles that are capable, similarly to the reticular ER, of taking up and releasing Ca2+. The vesicular sub-compartment of ER moved rapidly along the dendrites in both anterograde and retrograde directions at a velocity of 0.2-0.3 μm/second. Depletion of microtubules, overexpression of dominant-negative kinesin and kinesin depletion by antisense DNA reduced the number and velocity of the moving vesicles, suggesting that kinesin may drive the transport of the vesicular sub-compartment of ER along microtubules in the dendrite. Rapid transport of the Ca2+-releasable sub-compartment of ER might contribute to rapid supply of fresh ER proteins to the distal part of the dendrite, or to the spatial regulation of intracellular Ca2+ signaling.
A Differential Evolution for the Tuning of a Chess Evaluation Function

We describe an approach for tuning a chess program evaluation function. The general idea is based on the differential evolution algorithm. The tuning of the evaluation function has been implemented using only final outcomes of games. Each individual of the population represents a chess program with specific (different) parameters of its evaluation function. The principal objective is to ascertain fitness values of individuals in order to promote them into successive generations. This is achieved by competition between individuals of two populations which also changes the individuals of both populations. The preliminary results show that less generations are necessary to obtain good (tuned) parameters. Acquired results have exhibited the fact that population individuals (vectors) are not as diverse as they would be if no changes were made during the competition.
Acceleration through the Dirac-Pauli vacuum and effects of an external field.

The Dirac equation for a charged spin-1/2 particle with an anomalous magnetic moment μ' in a background magnetic field is solved in Rindler coordinates (uniformly accelerated frame). A method based on the existence of a spin operator is presented, which permits us to solve the Dirac-Pauli equation in curvilinear coordinates. This is applied to the Rindler coordinates, and the spectra of conserved quantities such as the energy, particle, and spin densities are calculated. The ratio of the energy and particle densities is not given by a Fermi-Dirac distribution, except in the limit μ'→0. Furthermore, the spectrum of the energy density takes a complicated form that cannot be simply interpreted as thermal even in the zero mass limit
Anthranilate degradation by a cold‐adapted Pseudomonas sp. 

An alpine soil bacterium Pseudomonas sp. strain PAMC 25931 was characterized as eurypsychrophilic (both psychrophilic and mesotolerant) with a broad temperature range of 5–30 °C both for anthranilate (2‐aminobenzoate) degradation and concomitant cell growth. Two degradative gene clusters (antABC and catBCA) were detected from a fosmid clone in the PAMC 25931 genomic library; each cluster was confirmed to be specifically induced by anthranilate. When expressed in Escherichia coli, the recombinant AntABC (anthranilate 1,2‐dioxygenase, AntDO) converted anthranilate into catechol, exhibiting strict specificity toward anthranilate. Recombinant CatA (catechol 1,2‐dioxygenase, C12O) from the organism was active over a broad temperature range (5–37 °C). However, CatA rapidly lost the enzyme activity when incubated at above 25 °C. For example, 1 h‐preincubation at 37 °C resulted in 100% loss of enzyme activity, while a counterpart from mesophilic Pseudomonas putida mt‐2 did not show any negative effect on the initial enzyme activity. These results suggest that CatA is a new cold‐adapted thermolabile enzyme, which might be a product through the adaptation process of PAMC 25931 to naturally cold environments and contribute to its ability to grow on anthranilate there.
Channels selection for motor imagery paradigm — An Itakura distance based method

An offline analysis method is proposed for a brain computer interface paradigm. Changes that appear in brain during the motor tasks should be reflected in the EEG signals. The sequences of EEG data are modeled by autoregressive (AR) processes. Based on Itakura distance (ID), the differences that occur during mental tasks (left and right hand movement imagination) versus relaxation period are measured. After applying statistical tests, channels selection is performed. The data contained in the chosen channels are classified with linear discriminant classifier (LDA), quadratic discriminant classifier (QDA) and Mahalanobis distance classifier (MD). The advantage of channels selection based on ID is that the picked channels contain relevant features. The effectiveness of the method is sustained by the classification rates obtained.
The Proangiogenic Capacity of Polymorphonuclear Neutrophils Delineated by Microarray Technique and by Measurement of Neovascularization in Wounded Skin of CD18-Deficient Mice

Growing evidence supports the concept that polymorphonuclear neutrophils (PMN) are critically involved in inflammation-mediated angiogenesis which is important for wound healing and repair. We employed an oligonucleotide microarray technique to gain further insight into the molecular mechanisms underlying the proangiogenic potential of human PMN. In addition to 18 known angiogenesis-relevant genes, we detected the expression of 10 novel genes, namely midkine, erb-B2, ets-1, transforming growth factor receptor-β2 and -β3, thrombospondin, tissue inhibitor of metalloproteinase 2, ephrin A2, ephrin B2 and restin in human PMN freshly isolated from the circulation. Gene expression was confirmed by the RT-PCR technique. In vivo evidence for the role of PMN in neovascularization was provided by studying neovascularization in a skin model of wound healing using CD18-deficient mice which lack PMN infiltration to sites of lesion. In CD18-deficient animals, neovascularization was found to be significantly compromised when compared with wild-type control animals which showed profound neovascularization within the granulation tissue during the wound healing process. Thus, PMN infiltration seems to facilitate inflammation-mediated angiogenesis which may be a consequence of the broad spectrum of proangiogenic factors expressed by these cells.
A New Multi-Resolution Wavelet Neural Network For Bridge Health Monitoring

Abstract Anew multi-resolution wavelet network for bridge health monitoring is proposed in this paper, which has one hidden layer and multiple outputs. In this network, training data and forecasting data collected from bridge health monitoring are important factors in determining structure. By learning algorithm the finite wavelets and scaling functions are selected for the active function. All outputs vary from the coarsest resolution to the finest one. The theoretical analysis shows that the finest output convergence becomes uniformly.
A practical implementation of ISMS

Nowadays, access to reliable information has become an essential factor leading to success in business. In this regard, adequate security of information and systems that process it is critical to the operation of all organizations. Therefore organizations must understand and improve the current status of their information security in order to ensure business continuity and increase rate of return on investments. Since, information security has a very important role in supporting the activities of the organization and for this reason; it is needed to have a standard or benchmark which regulates governance over information security. Hence, this paper discusses some of Information Security Management System (ISMS) standards in order to determine their strengths and challenges. Then, based on most appropriate standards in the field, a method is proposed to allow information technology-related or based enterprises to implement their ISMS. This method helps identifying critical assets and related threats and vulnerabilities, assessing assets risks and providing necessary risk treatment plans. The proposed method makes it possible and structured to establish information security management system in IT related large-scale enterprises.
Pattern distance of time series based on segmentation by important points

In order to analyze the changing trend of time series, a novel method is proposed in this paper, which supports fast searching similar trend sequence in time series. It first segments time series based on a series of perceptually important points, and then converts important point series into piecewise trend sequence (PTS). And a variable-step algorithm for subtrend sequence searching based on PTS is also proposed. The theoretic analysis and simulation indicate that the algorithm has better performance in time and space than classic ones.
Are Courts Slow? Exposing and Measuring the Invisible Determinants of Case Disposition Time

This article analyses civil case disposition time by developing hypotheses to explain behavioral and structural determinants of so-called 'delay' and suggesting a novel methodology ('Echronometrics') to account for factors, operating at both macro and micro socio-economic levels, that influence the behavior and outputs of civil courts. Our proposed methodology includes more relevant variables, and specifies their interdependence, thus offering a more powerful explanatory tool for future empirical research to account for and measure the complex interactions of time and cost in civil trials
Dopant Activation and Deactivation Phenomena during Advanced Millisecond Anneal Cycles

Achieving very high concentrations of electrically active dopants is essential for minimizing parasitic resistances in advanced CMOS devices. Existing methods are severely challenged by the limits on electrical activation of dopants imposed by their solid solubility, electrical deactivation, segregation at silicon/SiO2 interfaces and by the need to limit process temperatures to allow integration of new materials. This paper investigates the factors in the design of millisecond annealing (MSA) temperature cycles with peak temperatures below 1200ºC that can still improve electrical activation of high doses of As or P atoms ion-implanted in preamorphized silicon. High dose P doping was found to display better activation than could be achieved with As, where activation decreased for higher doses. MSA pulse length was found to have a weak influence on activation. Higher preheat temperatures degraded activation, as might be expected from the consequent increase in the post-pulse thermal budget. The effects of different cooling profiles after the pulsed heating were investigated, but were found to be insignificant for cooling cycle temperatures < 725ºC. The preheating conditions offer further room for optimization because they can affect dopant diffusion and activation during the regrowth of the amorphized layer.
Conspiracist Ideation as a Predictor of Climate-Science Rejection

Reanalysis of the survey data sets of Lewandowsky, Oberauer, and Gignac (2013) and Lewandowsky, Gignac, and Oberauer (2013) indicates that the conclusions of those articles—that conspiracist ideation predicts skepticism regarding the reality of anthropogenic climate change—are not supported by the data. Nonlinear relationships were overlooked in both analyses, and this resulted in model misspecification. The authors used structural equation modeling (SEM) assuming linear relationships between the variables, and it is essential to test this assumption (Bentler and Chou, 1987, p. 86; Ullman, 2007, p. 683). In this Commentary, we show, using nonparametric local regression, that this assumption does not hold for the relationship between conspiracist ideation and views on climate science, the relationship that produced one of the central claims of both articles and the majority of the press interest (e.g., Corner, 2012; Pearlman, 2012).
Medicaid Coverage, Methadone Maintenance, and Felony Arrests: Outcomes of Opiate Treatment in Two States

ABSTRACT A modest number of clinics in Oregon and Washington provide MMT maintenance treatment (MMT) services. More than 10,000 clients in each state were followed for 3 years after an initial admission for opiate use between 1993 and 2000. Medicaid clients in both states had far greater access to MMT than their non-Medicaid counterparts, controlling for differences in client characteristics using propensity scores. Months in MMT were associated with much lower arrest rates than time not in treatment, but unexpectedly this was only true for clients participating in MMT for many months. Despite differences in the treatment systems for opiate addiction in these two states observed in previous studies, the current findings generalized across both states.
The active transport of fluorescein by the retinal vessels and the retina

1. The movement of fluorescein across the retinal surface of the rabbit's eye was estimated by measuring the concentration gradient of the dye in the vitreous body. These measurements were made in vivo by means of a slit‐lamp fluorophotometer, or were taken from frozen sections of enucleated eyes.
Performance analysis of wavelet based denoise system for power quality disturbances

The performance analysis of wavelet based denoise system for power quality disturbances including sag, swell, flicker, harmonic, transient and notch is presented in this paper. The denoise algorithm uses Daubechie family wavelet and a universal soft threshold technique applied on the detailed wavelet coefficients based on its standard deviation and data length. This eliminates and reduces the noise content in the signal. Lastly, the inverse discrete wavelet transform is done to reconstruct and recover the denoised PQ disturbance signal. All the disturbance signals are contaminated with Additive White Gaussian Noise of initial noise SNR of 30dB. The wavelet based denoise system in this paper shows excellent performance for sag, swell and flicker, good performance for harmonic, moderate performance for transient and poor performance for notch. The performance analysis presented in this paper provides an understanding of how the power quality disturbance frequency contents and its magnitude effect the performance of the wavelet based denoise system.
Voltage-Mode All-Pass Filters Using Universal Voltage Conveyor and MOSFET-Based Electronic Resistors

The paper presents two novel realizations of voltage-mode first-order all-pass filters. Both circuits use single universal voltage conveyor (UVC), single capacitor, and two grounded resistors. Using the two NMOS transistors-based realizations of the electronic resistor with two symmetrical power supplies, presented all-pass filter circuits can be easily made electronically tunable. Proposed filter structures provide both inverting and non-inverting outputs at the same configuration simultaneously and they have high-input and low-output impedances that are desired for easy cascading in voltage-mode operations. The nonidealities of the proposed circuits are also analyzed and compared. The theoretical results of both circuits are verified by SPICE simulations using TSMC 0.35 μm CMOS process parameters. Based on the evaluation, the behavior of one of the circuits featuring better performance was also experimentally measured using the UVC-N1C 0520 integrated circuit.
The Consequences of Internet Cafe use on Turkish College Students' Social Capital.

This paper draws on a part of the doctoral research study that investigates the potential impacts of Internet cafe use on Turkish college students’ social capital. In this study, Internet cafe usage was portrayed by the amount of time spent and the frequency of online activities engaged at the cafes. Social capital, on the other hand, was characterized by feelings about loneliness, quality of social network with family, quality of social network with friends, and pro-social attitudes. A quantitative research design with a survey technique was employed. The data were collected from 758 undergraduate students from the College of Education of a major Anatolian university. Multiple linear regression with simple slope analysis were conducted to determine the proportion of variance that Internet cafe use accounts for in social capital and whether participants’ type of accommodation adds anything significant. Results indicated that (a) Internet cafe usage did not significantly influence on loneliness and prosocial attitudes, (b) spending more time at Internet cafes and frequent entertainment-based online activities led to a decrease in the quality of social networks with both family and friends, (c) frequent online communication activities led to an increase in the quality of social networks with friends, and (d) such effects were the same for participants who stay alone, stay with family, and stay with friends. It was concluded that Internet technology was more than a simple and neutral tool that may constitute complex social dimensions involving profound alterations for youth’s social life.
Web 2.0 technologies and workplace paradigms to enable e-professional workstyles

This paper explores current and emerging trends in collaborative working and investigates the role of Web 2.0 technologies in establishing networked forms of collaboration across organisational boundaries. The emerging Web 2.0 enabled workplace model recognizes that people are engaged in different tasks to be carried out in parallel, and are part of multiple projects, teams and even organizations. The new forms of collaborative networks enable people and their business environment to increase their productivity and leverage the creative potential of working collaboratively. In particular we explore the domain of managing complex projects. Based on examples of emerging forms of collaborative networks as well as case studies in actual collaborative work processes, we envisage new models of person-based collaboration and study the way they are embedded in organizational networks. The paper concludes in discussing open problems and proposing research themes in the area of networked collaboration of professionals.