Miracle-dz/UniFAR-FTUs huggingface dataset:
Human immune anomaly and misuse based detection for computer system operations: part II

This paper is a follow up on our previous work on intrusion detection for computer systems. It is motivated by the accelerated growth of computer viruses in computer networks. Most applications and operational systems are overwhelmed by security failures at different levels. If there are no verification and monitoring procedures in the computational system, then security services (such as access control, firewalls and cryptography) become ineffective. In this paper, we present a security system for fraud detection of intruders and misuse of a mail system in a computer network environment. Our intrusion detection model is inspired by the natural immune system. This paper discusses our model, and presents preliminary results for both anomaly and operational misuse of the computer system.
Androgen Receptor Exon 1 Mutation Causes Androgen Insensitivity by Creating Phosphorylation Site and Inhibiting Melanoma Antigen-A11 Activation of NH2- and Carboxyl-terminal Interaction-dependent Transactivation*

Background: Androgen receptor (AR) regulation of transcription is required for male genital development in utero. Results: An AR NH2-terminal mutation caused partial androgen insensitivity by creating a phosphorylation site and disrupting coregulator effects of melanoma antigen-A11 (MAGE-11). Conclusion: MAGE-11 is an obligatory coregulator during human male fetal genital development. Significance: AR signaling during the critical period of male fetal genital development requires MAGE-11. Naturally occurring germ line mutations in the X-linked human androgen receptor (AR) gene cause incomplete masculinization of the external genitalia by disrupting AR function in males with androgen insensitivity syndrome. Almost all AR missense mutations that cause androgen insensitivity syndrome are located in the highly structured DNA and ligand binding domains. In this report we investigate the functional defect associated with an AR exon 1 missense mutation, R405S, that caused partial androgen insensitivity. The 46,XX heterozygous maternal carrier had a wild-type Arg-405 CGC allele but transmitted an AGC mutant allele coding for Ser-405. At birth, the 46,XY proband had a bifid scrotum, hypospadias, and micropenis consistent with clinical stage 3 partial androgen insensitivity. Androgen-dependent transcriptional activity of AR-R405S expressed in CV1 cells was less than wild-type AR and refractory in androgen-dependent AR NH2- and carboxyl interaction transcription assays that depend on the coregulator effects of melanoma antigen-A11. This mutation created a Ser-405 phosphorylation site evident by the gel migration of an AR-R405S NH2-terminal fragment as a double band that converted to the wild-type single band after treatment with λ-phosphatase. Detrimental effects of the R405S mutation were related to the proximity of the AR WXXLF motif 433WHTLF437 required for melanoma antigen-A11 and p300 to stimulate transcriptional activity associated with the AR NH2- and carboxyl-terminal interaction. We conclude that the coregulator effects of melanoma antigen-A11 on the AR NH2- and carboxyl-terminal interaction amplify the androgen-dependent transcriptional response to p300 required for normal human male sex development in utero.
Multiple serially concatenated single parity check codes

Single parity check (SPC) codes are applied in a serial concatenation structure to produce a high performance coding scheme with very low complexity. We show a simple yet elegant way of improving the system performance at low bit error rates (BER), without changing the overall code parameters (code rate, code block length). We compare its BER performance to the best 64-state convolutional codes, and the 16-state turbo codes. Comparisons show that its performance is only slightly poorer. Despite this inferior performance, the scheme is very suitable for practical implementation due to its flexible code parameters. We conclude by showing that its decoding complexity is more than 1 order of magnitude less than that of a 16-state turbo code.
Comparison of CCTT split-winding and EE integrated magnetics for high-power dc-dc converters

This paper investigates integrated magnetic structures for high-power dc-dc converters. A novel CCTT split-winding integrated magnetic (IM) is introduced and is compared to a conventional EE IM structure. The CCTT IM allows for a reduction in external dc inductance in comparison to the EE design. This reduced dc inductance allows for a size/volume reduction. Low-power-loss ferrite is used and this results in a very efficient design. An IM design flowchart is presented which allows for quick CCTT and EE IM designs. The flow chart incorporates fringing equations to determine the dc and ac inductance of the IM structure, thus allowing for a more accurate design. The volume vs. efficiency for both magnetic structures is compared for various values of input current ripple. 3D FEA is used to fine tune the IM designs. High-power, 80 kW, experimental results validate the CCTT IM concept and show that the magnetic component is 99.7 % efficient over the input power range.
Joint Data Detection and Channel Estimation for Fading Unknown Time-Varying Doppler Environments

This work considers a joint channel estimation and data detection technique for Multiple Space-Time Trellis Codes (MSTTCs) operating over unknown time-varying channels with large Doppler spread. We propose an algorithm, called Doppler Adaptive Smoothed Data Detection and Kalman Estimation (DA-SDD-KE), that jointly detects data and estimates the channel as well as the time-varying Doppler. In this scheme, an Adaptive Kalman Predictor (AKP) consisting of a KP and a covariance-based Doppler estimator is incorporated into a Per-Survivor Processing (PSP)-based algorithm that utilizes the past, present and future received symbols for smoothed data detection. For comparison purposes, we also develop a Doppler Adaptive version of the Delayed Mixture Kalman Filtering (DMKF) technique, referred to as DA-DMKF, where the adaptive estimations of the channel and the Doppler shift are based on sequences of importance samples. Moreover, we propose a model for generating a Rayleigh fading process with time-varying Doppler using the sum of sinusoids method. The performance of the DA-SDD-KE and DA-DMKF algorithms over channels with constant, linear and quadratic Doppler functions is evaluated using computer simulations, revealing that the DA-SDD-KE algorithm performs well for all considered Doppler functions, and provides a considerably gain over the DA-DMKF algorithm.
Robust H∞ Load Frequency Control of future power grid with energy storage considering parametric uncertainty and time delay

In future grid environment, the unpredictable fluctuating characteristics of the renewable energy (RE) sources will inevitably cause adverse impacts on the system dynamic performance such as jeopardizing the frequency stability. Meanwhile, the utilization of energy storage (ES) can play a positive and noteworthy role in dealing with above problems. In this paper, we analyze the dynamic model of the grid-connected ES and the closed-loop frequency-domain block diagram is developed. Then a novel controller is proposed based on rigorous linear matrix inequalities (LMI) theory to ensure the robustness and stability of the system, when taking into account the parametric uncertainty and random time delay in control channels in the complex environment. The system can utilize ES to participate in Load Frequency Control (LFC) process so as to assist conventional thermal units. Finally, comprehensive case studies are performed to demonstrate the effectiveness of the proposed methods.
Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites.

BACKGROUND The currently used standards for human chorionic gonadotropin (hCG) and its alpha and beta subunits (hCGalpha and hCGbeta) contain substantial amounts of contaminating variants of hCG and other impurities. Furthermore, some partially degraded forms of hCG and its subunits have become of potential clinical importance, e.g., "nicked" forms of hCG (hCGn) and hCGbeta (hCGbetan), which contain cuts in the peptide backbone between amino acids 44-45 or 47-48 in hCGbeta, and a fragment of hCGbeta (hCGbetacf) consisting of amino acids 6-40 and 55-92 bound together by disulfide bridges. The IFCC appointed a working group with the aim of preparing new standards for hCG and related substances to improve standardization of their immunoassays. METHODS Large amounts of hCG and its subunits as well as of hCGn, hCGbetan, and hCGbetacf were prepared by previously developed purification methods in combination with hydrophobic interaction chromatography and reversed-phase HPLC. Each preparation was characterized on the basis of amino acid and sequence analyses, carbohydrate composition, and electrophoretic patterns. Immunoassays for relevant contaminating proteins were also performed. RESULTS The major preparations were homogeneous and free of contaminating proteins. Concentrations of the final preparations were determined by amino acid analysis. CONCLUSIONS Calibrated in substance concentrations (mol/L) based on amino acid analyses, these preparations will facilitate improved standardization of immunoassays for hCG and its metabolites. The six preparations have now been established by the WHO as new 1st Reference Reagents for immunoassays with the following codes: hCG 99/688, hCGbeta 99/650, hCGalpha 99/720, hCGn 99/642, hCGbetan 99/692, and hCGbetacf 99/708. In contrast to the 3rd International Standard (75/537), the clinically most important Reference Reagent for hCG (99/688) contains no hCGn and negligible amounts of free subunits.
Propriospinal neurons contribute to bulbospinal transmission of the locomotor command signal in the neonatal rat spinal cord

This study examines whether propriospinal transmission contributes to descending propagation of the brainstem locomotor command signal in the in vitro neonatal rat spinal cord. Using double bath partitions, synaptic transmission was suppressed in the cervicothoracic region while monitoring locomotor‐like activity on lumbar ventral roots evoked by either chemical or electrical stimulation of the brainstem. Locomotor‐like activity induced by electrical stimulation was more stable (cycle period coefficient of variation (CV) 11.7 ± 6.1%) than the rhythm induced by chemical stimulation (CV 31.3 ± 6.4%). Ca2+‐free bath solution, elevated Mg2+ ion concentration, excitatory amino acid receptor antagonists (AP5 and/or CNQX), and the muscarinic receptor antagonist, atropine, were used in attempts to block synaptic transmission. Each of these manipulations, except muscarinic receptor blockade, was capable of blocking locomotor‐like activity induced by brainstem stimulation. However, locomotor‐like activity induced by higher intensity electrical stimulation of the brainstem (1.2–5 times threshold) was relatively refractory to synaptic suppression using AP5 and CNQX, and Ca2+‐free solution was more effective if combined with high Mg2+ (15 mm) or EGTA. Enhancement of neuronal excitation in the cervicothoracic region, using Mg2+‐free bath solution, facilitated brainstem activation of locomotor‐like activity in the lumbar cord, consistent with a propriospinal mechanism of locomotor signal propagation. Blockade of brainstem‐induced locomotor‐like activity was related to the number of cervicothoracic segments exposed to synaptic suppression, being most effective if five or more segments were included. These results provide direct evidence that propriospinal pathways contribute to bulbospinal activation of the locomotor network in the in vitro neonatal rat brainstem–spinal cord preparation, and suggest that a propriospinal system is recruited in parallel with long direct projections that activate the locomotor network.
Chloroplast 2010: A Database for Large-Scale Phenotypic Screening of Arabidopsis Mutants1[W][OA]

Large-scale phenotypic screening presents challenges and opportunities not encountered in typical forward or reverse genetics projects. We describe a modular database and laboratory information management system that was implemented in support of the Chloroplast 2010 Project, an Arabidopsis (Arabidopsis thaliana) reverse genetics phenotypic screen of more than 5,000 mutants (http://bioinfo.bch.msu.edu/2010_LIMS; www.plastid.msu.edu). The software and laboratory work environment were designed to minimize operator error and detect systematic process errors. The database uses Ruby on Rails and Flash technologies to present complex quantitative and qualitative data and pedigree information in a flexible user interface. Examples are presented where the database was used to find opportunities for process changes that improved data quality. We also describe the use of the data-analysis tools to discover mutants defective in enzymes of leucine catabolism (heteromeric mitochondrial 3-methylcrotonyl-coenzyme A carboxylase [At1g03090 and At4g34030] and putative hydroxymethylglutaryl-coenzyme A lyase [At2g26800]) based upon a syndrome of pleiotropic seed amino acid phenotypes that resembles previously described isovaleryl coenzyme A dehydrogenase (At3g45300) mutants. In vitro assay results support the computational annotation of At2g26800 as hydroxymethylglutaryl-coenzyme A lyase.
Yersinia enterocolitica ClpB Affects Levels of Invasin and Motility

ABSTRACT Expression of the Yersinia enterocolitica inv gene is dependent on growth phase and temperature. inv is maximally expressed at 23°C in late-exponential- to early-stationary-phase cultures. We previously reported the isolation of a Y. enterocolitica mutant (JB1A8v) that shows a decrease in invasin levels yet is hypermotile when grown at 23°C. JB1A8v has a transposon insertion within uvrC. Described here is the isolation and characterization of a clone that suppresses these mutant phenotypes of the uvrC mutant JB1A8v. This suppressing clone encodes ClpB (a Clp ATPase homologue). The Y. enterocolitica ClpB homologue is 30 to 40% identical to the ClpB proteins from various bacteria but is 80% identical to one of the two ClpB homologues ofYersinia pestis. AclpB::TnMax2 insertion mutant (JB69Qv) was constructed and determined to be deficient in invasin production and nonmotile when grown at 23°C. Analysis ofinv and fleB (flagellin gene) transcript levels in JB69Qv suggested that ClpB has both transcriptional and posttranscriptional effects. In contrast, a clpB null mutant, BY1v, had no effect on invasin levels or motility. A model accounting for these observations is presented.
Refinement of Object-Z Specifications Using Morgan-s Refinement Calculus

— Morgan’s refinement calculus (MRC) is one of the well-known methods allowing the formality presented in the program specification to be continued all the way to code. On the other hand, Object-Z (OZ) is an extension of Z adding support for classes and objects. There are a number of methods for obtaining code from OZ specifications that can be categorized into refinement and animation methods. As far as we know, only one refinement method exists which refines OZ specifications into code. However, this method does not have fine-grained refinement rules and thus cannot be automated. On the other hand, existing animation methods do not present mapping rules formally and do not support the mapping of several important constructs of OZ, such as all cases of operation expressions and most of constructs in global paragraph. In this paper, with the aim of providing an automatic path from OZ specifications to code, we propose an approach to map OZ specifications into their counterparts in MRC in order to use fine-grained refinement rules of MRC. In this way, having counterparts of our specifications in MRC, we can refine them into code automatically using MRC tools such as RED. Other advantages of our work pertain to proposing mapping rules formally, supporting the mapping of all important constructs of Object-Z, and considering dynamic instantiation of objects while OZ itself does not cover this facility.
Accuracy of a third (Dolphin Voyager) versus first generation pulse oximeter (Nellcor N-180) in predicting arterial oxygen saturation and pulse rate in the anesthetized dog.

OBJECTIVE To compare the accuracy of a 3rd (Dolphin Voyager) versus 1st generation pulse oximeter (Nellcor N-180). STUDY DESIGN Prospective laboratory investigation. ANIMALS Eight adult dogs. METHODS In anesthetized dogs, arterial oxygen saturation (SpO(2)) was recorded simultaneously with each pulse oximeter. The oxygen fraction in inspired gas (FiO(2)) was successively reduced from 1.00 to 0.09, with re-saturation (FiO(2) 0.40) after each breathe-down step. After each 3-minute FiO(2) plateau, SpO(2) and pulse rate (PR) were compared with the fractional arterial saturation (SaO(2)) and PR determined by co-oximetry and invasive blood pressure monitoring, respectively. Data analysis included Bland-Altman (B-A) plots, Lin's concordance correlation factor (rho(c)), and linear regression models. RESULTS Over a SaO(2) range of 33-99%, the overall bias (mean SpO(2) - SaO(2)), precision (SD of bias), and accuracy (A(rms)) for the Dolphin Voyager and Nellcor N-180 were 4.3%, 4.4%, and 6.1%, and 3.2%, 3.0%, and 4.3%, respectively. Bias increased at SaO(2) < 90%, more so with the Dolphin Voyager (from 1.6% to 8.6%) than Nellcor N-180 (from 3.2% to 4.5%). The SpO(2) readings correlated significantly with SaO(2) for both the Dolphin Voyager (rho(c) = 0.94) and Nellcor N-180 (rho(c) = 0.97) (p < 0.001). Regarding PR, bias, precision, and accuracy (A(rms)) for the Dolphin Voyager and Nellcor N-180 were -0.5, 4.6, and 4.6 and 1.38, 4.3, and 4.5 beats minute(-1), respectively. Significant correlation existed between pulse oximeter and directly measured PR (Dolphin Voyager: rho(c) = 0.98; Nellcor N-180: rho(c) = 0.99) (p < 0.001). CONCLUSIONS AND CLINICAL RELEVANCE In anesthetized dogs with adequate hemodynamic function, both instruments record SaO(2) relatively accurately over a wide range of normal saturation values. However, there is an increasing overestimation at SaO(2) < 90%, particularly with the Dolphin Voyager, indicating that 3rd generation pulse oximeters may not perform better than older instruments. The 5.4-fold increase in bias with the Dolphin Voyager at SaO(2) < 90% stresses the importance of a 93-94% SpO(2) threshold to ensure an arterial saturation of >or=90%. In contrast, PR monitoring with both devices is very reliable.
How Does Child Labor Affect the Demand for Adult Labor?

Do employers substitute adults for children, or do they treat them as complements? Using data from a Mexican schooling experiment, I find that decreasing child farmwork is accompanied by increasing adult labor demand. This increase was not caused by treatment money reaching farm employers: there were no significant increases in harvest prices and quantities, nonlabor inputs, or nonfarm labor supply. Furthermore, coordinated movements in price and quantity can distinguish this increase in demand from changes in supply induced by the treatment’s income effects. Thus, declining child supply caused increasing adult demand: employers substituted adults for children.
The sialoadhesin (CD169) expressing a macrophage subset in human proliferative glomerulonephritis.

BACKGROUND Sialoadhesin (Sn; CD169) is a lectin-like receptor whose expression is restricted to subsets of tissue and inflammatory macrophages. We have previously identified accumulation of Sn+ macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Sn+ macrophages in human proliferative glomerulonephritis. METHODS Frozen kidney sections from normal adult human kidney (n = 4) and pediatric nephropathy (n = 40) were stained for total macrophages (CD68+ cells), Sn+ macrophages, CD3+ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. RESULTS Sn+ macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n = 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Sn+ macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Sn+ cells are a subset of total CD68+ macrophages. The number of glomerular Sn+ macrophages correlated with the degree of proteinuria and glomerular lesions (r = 0.44, P = 0.0045 and r = 0.82, P<0.0001; respectively), while interstitial Sn+ macrophages correlated with the degree of proteinuria and interstitial damage (r = 0.59, P<0.0001 and r = 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Sn+ macrophages and CD3+ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Sn+ macrophages and CD3+ T-cells (r = 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Sn+ macrophages. CONCLUSION This study has identified Sn+ cells as a macrophage subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Sn+ macrophages may play an important role in progressive renal disease.
Investigation of optimal cues to instruction for pelvic floor muscle contraction: A pilot study using 2D ultrasound imaging in pre‐menopausal, nulliparous, continent women

Optimal urethrovesical positioning (UVP) may be important for continence. Pelvic floor muscle contraction (PFMC) influences UVP. PFMC instruction cues vary and often encourage anterior PFM recruitment that may result in sub‐maximal posterior facilitation. Study hypothesis: posterior or combined cues are more influential in optimizing UVP during PFMC following a brief practice period than anterior cue.
Low-voltage CMOS DC-DC converters for energy harvesting applications

Environmental energy harvesting has attracted much attention as a possible power source for remote electronic devices and implanted/embedded electronics. Low-voltage DC-DC charge pump is the key component for ultralow-power energy harvesting. This work addresses some issues related to low-voltage DC-DC converter based on the mainstream CMOS technology with an example of high-efficient and excellent loading performance cross-coupled charge pump by employing clock-boosting, forward-biasing, pulse-skipping modulation techniques.
Pig-shed air polluted by α-haemolytic cocci and ammonia causes subclinical disease and production losses

There is mounting evidence that bacteria originating from pigs degrade the environment of the pig shed and adversely affect the health of the animals and the pig-shed workers. α-haemolytic cocci (AHC) occur in pig-shed environments, but are regarded as commensals. Ammonia is also a component of the pig-shed environment, and is known to damage upper respiratory tract epithelia. The aim of this study was to determine whether polluted air in pig sheds adversely affected performance indicators in pigs. Modelling revealed a direct effect of AHC on voluntary feed intake and hence AHC are not commensal. No direct effect of ammonia on the pigs was detected, but the combination of AHC and ammonia stimulated the immune system in a progressive manner, and there were direct effects of immune stimulation on food intake and growth resulting in poorer feed-conversion efficiency, even though the effects remained subclinical. The authors conclude that exposure of the respiratory epithelia of pigs to viable AHC in the presence of ammonia redirects nutrients away from production and towards the immune system, explaining the impact of poor pig-shed hygiene on production parameters.