Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptional control of glucoamylase synthesis in vegetatively growing and sporulating Saccharomyces species

Three unlinked, homologous genes, STA1, STA2, and STA3, encode the extracellular glycosylated glucoamylase isozymes I, II, and III, respectively, in Saccharomyces species. S. cerevisiae, which is sta0 (absence of functional STA genes in haploids), does carry a glucoamylase gene, delta sta, expressed only during sporulation (W. J. Colonna and P. T. Magee, J. Bacteriol. 134:844-853, 1978; I. Yamashita and S. Fukui, Mol. Cell. Biol. 5:3069-3073, 1985). In this study we examined some of the physiological and genetic factors that affect glucoamylase expression. It was found that STA2 strains grown in synthetic medium produce glucoamylase only in the presence of either Maltrin M365 (a mixture of maltooligosaccharides) or starch. Maximal levels of glucoamylase activity were found in cells grown in rich medium supplemented with glycerol plus ethanol, starch, or Maltrin. When various sugars served as carbon sources they all supported glucoamylase synthesis, although at reduced levels. In any given growth medium glucoamylase isozyme II synthesis was modulated by functionality of the mitochondria. Synthesis of glucoamylase is continuous throughout the growth phases, with maximal secretion taking place in the early stationary phase. In the various regimens, the differences in enzyme accumulation are accounted for by differences in the levels of glucoamylase mRNA. Both glucoamylase mRNA and enzyme activity were drastically and coordinately inhibited in MATa/MAT alpha diploids and by the presence of the regulatory gene STA10. Both effects were partially overcome when the STA2 gene was present on a multicopy plasmid. The STA2 mRNA and glucoamylase were coinduced in sporulating STA2/STA2 diploids. A smaller, coinduced RNA species was also detected by Northern blotting with a STA2 probe. The same mRNA species was detected in sporulating sta0 diploids and is likely to encode the sporulation-specific glucoamylase.
Drones as a Tool for Monoculture Plantation Assessment in the Steepland Tropics

Smallholder tree plantations are expanding in the steepland tropics due to demand for timber and interest in ecosystem services, such as carbon storage. Financial mechanisms are developing to compensate vegetation carbon stores. However, measuring biomass—necessary for accessing carbon funds—at small scales is costly and time-intensive. Therefore, we test whether low-cost drones can accurately estimate height and biomass in monoculture plantations in the tropics. We used Ecosynth, a drone-based structure from motion technique, to build 3D vegetation models from drone photographs. These data were filtered to create a digital terrain model (DTM) and digital surface model (DSM). Two different canopy height models (CHMs) from the Ecosynth DSM were obtained by subtracting terrain elevations from the Ecosynth DTM and a LIDAR DTM. We compared height and biomass derived from these CHMs to field data. Both CHMs accurately predicted the height of all species combined; however, the CHM from the LiDAR DTM predicted heights and biomass on a per-species basis more accurately. Height and biomass estimates were strong for evergreen single-stemmed trees, and unreliable for small leaf-off species during the dry season. This study demonstrates that drones can estimate plantation biomass for select species when used with an accurate DTM.
Design and Control of a Novel 3-DOF Parallel Robot

This paper proposes a new configuration of a 3 DOF parallel robot which uses revolute and spherical joints. It also presents the inverse kinematic model and proposes a PID-type controller with tracking to asymptotically track a desired reference trajectory. This 3 DOF parallel robot can be utilized as a motion simulator or ankle rehabilitator robot. Some simulations were performed with the virtual prototype in MD ADAMS software to verify the performance of the PID-type controller. Finally, preliminary experimental tests using a physical prototype are presented as well.
Demonstration of coherent time-frequency Schmidt mode selection using dispersion-engineered frequency conversion

Time-frequency Schmidt (TFS) modes of ultrafast quantum states are naturally compatible with high bit-rate integrated quantum communication networks. Thus they offer an attractive alternative for the realization of high dimensional quantum optics. Here, we present a quantum pulse gate based on dispersion-engineered ultrafast frequency conversion in a nonlinear optical waveguide, which is a key element for harnessing the potential of TFS modes. We experimentally retrieve the modal spectral-temporal structure of our device and demonstrate a single-mode operation fidelity of 80\%, which is limited by experimental shortcomings. In addition, we retrieve a conversion efficiency of 87.7\% with a high signal-to-noise ratio of 8.8 when operating the quantum pulse gate at the single-photon level.
Performance and scalability of voldemort NoSQL

Currently companies need database engines able to process and store massive quantity of data. Traditional databases due to the large amount of unstructured data have trouble processing this amount of data in an acceptable time for businesses. To solve these problems recently emerged the NoSQL databases, offering great performance, flexibility and scalability. In this paper we evaluate the performance and scalability of a popular NoSQL database, Voldemort, using Yahoo! Cloud Serving Benchmark.
A Wideband Magneto-Electric Dipole Antenna

A new wideband magneto-electric dipole antenna composed of a horizontal bowtie electric dipole and a vertically oriented folded shorted patch antenna is designed. The antenna is excited by a stair-shaped probe feed without the need of an additional balun. A prototype was fabricated and measured. Results show that an impedance bandwidth of 95.2%, with <formula formulatype="inline"> <tex Notation="TeX">${\rm SWR}\leq 2$</tex></formula> from 1.65 GHz to 4.65 GHz, was achieved. Stable radiation pattern with low cross polarization, low back radiation, nearly identical E- and H-plane patterns and an antenna gain of 7.9 <formula formulatype="inline"><tex Notation="TeX">$\pm$</tex></formula> 0.9 dBi was found over the operating frequencies.
Minimizing tracking error while restricting the number of assets

Tracking error minimization is commonly used by the traditional passive fund managers as well as alternative portfolio (for example, hedge fund) managers. We propose a graduated non-convexity method to minimize portfolio tracking error with the total number of assets no greater than a specified integer K. The solution of this tracking error minimization problem is the global minimizer of the sum of the tracking error function and the discontinuous counting function. We attempt to track the globally minimal tracking error portfolio by approximating the discontinuous counting function with a sequence of continuously differentiable non-convex functions, a graduated non-convexity process. We discuss the advantages of this approach, present numerical results, and compare it with two methods from recent literature.
Scale independent raga identification using chromagram patterns and swara based features

In Indian classical music a raga describes the constituent structure of notes in a musical piece. In this work, we investigate the problem of scale independent automatic raga identification by achieving state-of-the-art results using GMM based Hidden Markov Models over a collection of features consisting of chromagram patterns, mel-cepstrum coefficients and timbre features. We also perform the above task using 1) discrete HMMs and 2) classification trees over swara based features created from chromagrams using the concept of vadi of a raga. On a dataset of 4 ragas- darbari, khamaj, malhar and sohini; we have achieved an average accuracy of ~ 97%. This is a certain improvement over previous works because they use the knowledge of scale used in the raga performance. We believe that with a more careful selection of features and by fusing results from multiple classifiers we should be able to improve results further.
The NASA EPOXI mission of opportunity to gather ultraprecise photometry of known transiting exoplanets

Abstract The NASA Discovery mission EPOXI, utilizing the Deep Impact flyby spacecraft, comprises two phases: EPOCh (Extrasolar Planet Observation and Characterization) and DIXI (Deep Impact eXtended Investigation). With EPOCh, we use the 30-cm high resolution visible imager to obtain ultraprecise photometric light curves of known transiting planet systems. We will analyze these data for evidence of additional planets, via transit timing variations or transits; for planetary moons or rings; for detection of secondary eclipses and the constraint of geometric planetary albedos; and for refinement of the system parameters. Over a period of four months, EPOCh observed four known transiting planet systems, with each system observed continuously for several weeks. Here we present an overview of EPOCh, including the spacecraft and science goals, and preliminary photometry results.
The Effect of Intrinsic Motivation and Sport Commitment on the Performance of Iranian National Water Polo Team

The purpose of the present study was to investigate the effect of intrinsic motivation and sport commitment on the performance of Iranian national water polo team. Statistical populations of this study included the whole men players invited to the camp of Iranian national water polo team (N=28). The statistical sample has been selected equal to the sampling group. After confirmation of virtual and contextual validity of intrinsic motivation questionnaires (McAuley et al) and sport commitment model scale (SCMS) by the experts, their reliability in a guide study and via Cronbach’s alpha coefficient were calculated respectively equal to �=0.83 and �=0.92 for the intrinsic motivation and sport commitment questionnaires. For analyzing the data, one-sample Kolmogorov-Smirnov test, one-way analysis of variance (ANOVA) and Tukey post hoc test were used in significance level of P�0.05. Results showed there was a significant difference between sport commitment values of successful, less successful and unsuccessful players and there was no significant difference between the intrinsic motivation values of successful, less successful and unsuccessful players. Also the results indicated there is a significant difference between the sport commitment of water polo players and high, middle and low sport records but there is no significant difference between the intrinsic motivation of water polo players and high, middle and low sport records. According to the study summary, it seems the players’ sport commitment as one of the individual characteristics effective on the team performance may have an important role in success and upraising the sport teams’ performance level.
Redox‐ and Hypoxia‐Responsive MRI Contrast Agents

The development of responsive or “smart” magnetic resonance imaging (MRI) contrast agents that can report specific biomarker or biological events has been the focus of MRI contrast agent research over the past 20 years. Among various biological hallmarks of interest, tissue redox and hypoxia are particularly important owing to their roles in disease states and metabolic consequences. Herein we review the development of redox‐/hypoxia‐sensitive T1 shortening and paramagnetic chemical exchange saturation transfer (PARACEST) MRI contrast agents. Traditionally, the relaxivity of redox‐sensitive Gd3+‐based complexes is modulated through changes in the ligand structure or molecular rotation, while PARACEST sensors exploit the sensitivity of the metal‐bound water exchange rate to electronic effects of the ligand‐pendant arms and alterations in the coordination geometry. Newer designs involve complexes of redox‐active metal ions in which the oxidation states have different magnetic properties. The challenges of translating redox‐ and hypoxia‐sensitive agents in vivo are also addressed.
A Novel Distributed Brillouin Sensor Based on Optical Differential Parametric Amplification

We propose and demonstrate a novel distributed sensor based on coherent interaction of the Brillouin gain and loss via optical differential parametric amplification (ODPA), which provides narrowed parametric Brillouin gain spectrum, strong Brillouin signal via differential gain, and short measurement time simultaneously. In experiment, a 0.5 m spatial resolution with a strain accuracy of 6 με is realized by using 20/15 ns Stokes and anti-Stokes pulse pair in a 15 m polarization-maintaining fiber. The spectrum narrowing phenomenon in ODPA Brillouin sensor is discussed.
A Levenberg-Marquardt Neural Network Model with Rough Set for Protecting Citrus from Frost Damage

The protection of citrus from night frosts is a recurrent and important issue that has been researched for many years. Although some feasible methods can be used to protect against and prevent frost, they should be implemented before the frost actually occurs. Therefore, how to accurately predict the temperature change in advance is a core problem for protecting citrus from frost damage. This paper proposes a new method, which combines the neural network with rough set based on the conditional information entropy, in order to improve the accuracy of temperature prediction. Utilizing attribute reduction drawing on the theory of rough set, the weak interdependency in the neural network can be decreased and the prediction accuracy can be increased. Some experiments show that the ability of a neural network to accurately predict minimum temperature can be improved through attribute reduction.
Pulmonary Emphysema and α1-Antitrypsin Deficiency

Of 72 patients with radiological evidence of pulmonary emphysema, emphysema occurred either alone or in association with bronchitis in 61, and 8 of these (13%) were found to have α1-antitrypsin deficiency. The main features of this condition are: exertional dyspnoea of relatively early onset (generally between 30 and 45 years of age), severely impaired FEV1 and TLCO, and radiological emphysema predominantly affecting the lower zones of the lungs. It is probable that any patient with all the above abnormalities has α1-antitrypsin deficiency. There is evidence to suggest that cigarette smoking may hasten the onset of this type of emphysema.
Color Recovery after Photoconversion of H2B::mEosFP Allows Detection of Increased Nuclear DNA Content in Developing Plant Cells1[W][OA]

Many higher plants are polysomatic whereby different cells possess variable amounts of nuclear DNA. The conditional triggering of endocycles results in higher nuclear DNA content (C value) that in some cases has been correlated to increased cell size. While numerous multicolored fluorescent protein (FP) probes have revealed the general behavior of the nucleus and intranuclear components, direct visualization and estimation of changes in nuclear-DNA content in live cells during their development has not been possible. Recently, monomeric Eos fluorescent protein (mEosFP) has emerged as a useful photoconvertible protein whose color changes irreversibly from a green to a red fluorescent form upon exposure to violet-blue light. The stability and irreversibility of red fluorescent mEosFP suggests that detection of green color recovery would be possible as fresh mEosFP is produced after photoconversion. Thus a ratiometric evaluation of the red and green forms of mEosFP following photoconversion could be used to estimate production of a core histone such as H2B during its concomitant synthesis with DNA in the synthesis phase of the cell cycle. Here we present proof of concept observations on transgenic tobacco (Nicotiana tabacum) Bright Yellow 2 cells and Arabidopsis (Arabidopsis thaliana) plants stably expressing H2B::mEosFP. In Arabidopsis seedlings an increase in green fluorescence is observed specifically in cells known to undergo endoreduplication. The detection of changes in nuclear DNA content by correlating color recovery of H2B::mEosFP after photoconversion is a novel approach involving a single FP. The method has potential for facilitating detailed investigations on conditions that favor increased cell size and the development of polysomaty in plants.
OUTBREAK OF A CHLAMYDOPHILA PSITTACI INFECTION IN LABORATORY RATS

During a study on Trypanosoma equiperdum propagation in adult male Wistar rats, four rats died spontaneously. Two of the dead animals were subjected to post mortem examination. From different organs of the rats, chlamydiae were isolated and confirmed by PCR and DNA sequencing as Chlamydophila psittaci. The results show that infection with Chlamydophila psittaci may occur in laboratory rats. Such outbreaks may have influence on the results of experimental studies. Chlamydial infections in laboratory animals also pose risk to humans (zoonosis).
Discrete Sine and Cosine Transforms for Signal Processing Spectral Overlap Saliencies of Induction Machine

A novel separation procedure applied to overlap condition of saliencies in induction machine is presented. The machine's current reaction to voltage step excitation is used to obtain saliency signals. The proposed procedure is based on a digital signal processing implementation of the discrete sine and cosine transforms for extracting the slotting saliency signal at close and equal frequencies of the slotting and saturation saliencies under heavy load operation. The proposed algorithms are processed based on an online adaptive threshold. Using the proposed separation algorithm guarantees precise estimation of rotor/flux angle and efficient sensorless control of the induction machine. A sensorless induction motor drive is implemented in the laboratory. Extensive experimental results are presented. A performance comparison between the proposed algorithm and previous works is provided. The robustness and effectiveness of the applied signal processing algorithms for sensorless torque control are verified at low speed and heavy load.
HBS and HFS feature selection methods for Chinese folk music classification

In this paper, we perform an exploring on music characteristics of Chinese folk music, in order to do targeted music restoration and perform digital reproduction on music segments. Starting from the point of music classification and music feature selection, we firstly choose SVM as the classifier according to experiment results of different classifiers. Then we introduce two common filter-filter methods: ReliefF-PCA and ReliefF-CA, and put forward to two heuristic filter-wrapper methods: HBS and HFS. At last, we apply these feature selection methods on our music data set and test their performance through experiments. The results show that HBS and HFS algorithms can effectively reduce the dimension of features vector and improve the classification accuracy, while other common feature selection methods perform poorer than them.
Planning with Numeric Timed Initial Fluents

Numeric Timed Initial Fluents represent a new feature in PDDL that extends the concept of Timed Initial Literals to numeric fluents. They are particularly useful to model independent functions that change through time and influence the actions to be applied. Although they are very useful to model real world problems, they are not systematically defined in the family of PDDL languages and they are not implemented in any generic PDDL planner, except for POPF2 and UPMurphi. In this paper we present an extension of the planner POPF2 (POPF-TIF) to handle problems with numeric Timed Initial Fluents. We propose and evaluate two contributions: the first is based on improvements of the heuristic evaluation, while the second considers alternative search algorithms based on a mixture of Enforced Hill Climbing and Best First Search.
Thoughts on screening tests in bacteriology.

Screening tests in bacteriology are not new. However, recent mechanized and automated techniques have greatly increased our technical capacity in this respect, which could lead to an uncritical extension of screening, with the accumulation of large amounts of data which are impossible to assess. Normal values must first be established and this may require detailed surveys of, for example, the antibody levels found in populations and the significance or lack of significance of particular bacteria or viruses in the material from which they are isolated. The uses to which screening tests are put need care. In Britain, the labour of routine screening of food handlers for enteric pathogens is considered scarcely worth while except in special circumstances such as contact of the food handler with relevant infectious disease (Howie, 1970). Those who press for such vast screening programmes need to say if the food handlers should be screened annually, monthly, or dailyand why one interval is chosen rather than another. On the other hand, the screening of waterworks employees to detect typhoid and paratyphoid carriers is probably worth doing because the work is not on such a scale as seriously to divert resources from more productive use.
A Screening Methodology for VMIN Drift in SRAM Arrays with Application to Sub-65nm Nodes

SRAMs are an integral part of system on chip devices. With transistor and gate length scaling to 65nm/45nm nodes, SRAM stability across the product's lifetime has become a challenge. Negative bias temperature instability, defects, or other phenomena that may manifest itself as a transistor threshold voltage (VT) increase can result in VMIN drift of SRAM memory cells through burn-in and/or operation. A direct assessment at time-zero is difficult because the transistor VT has not yet shifted, and therefore no capability to screen VMIN shift at time zero can be developed. This work describes a methodology developed on 65nm low power and high performance process technologies at Texas Instruments for screening SRAM cells at time zero before they become reliability issues
Non-surgical myocardial reduction in hypertrophic obstructive cardiomyopathy.

BACKGROUND Percutaneous transluminal septal ablation was recently introduced as an alternative to surgical treatment of hypertrophic obstructive cardiomyopathy. In this procedure, alcohol is injected into a proximal septal artery to create a localized myocardial infarction. OBJECTIVES To characterize the immediate and medium-term results following PTSMA. METHODS Of 13 patients referred for PTSMA, 8 were found suitable for the procedure. Hemodynamic parameters were evaluated prior to and following the procedure, and clinical and echo-Doppler parameters at 2 weeks and 9 months later. RESULTS The procedure was technically successful in all patients. Resting left ventricular outflow gradient at rest (by Doppler) fell from 82 +/- 37 to 15 +/- 8 mmHg (P < 0.001) 9 months later. Late post-procedural gradient after the Valsalva maneuver was 2 +/- 24 mmHg. The degree of mitral regurgitation fell from 2.0 +/- 0 to 1.5 +/- 0.5 (P < 0.05). New York Heart Association class for dyspnea improved from 2.8 +/- 0.5 to 1.8 +/- 0.8 (P < 0.01) and Canadian Cardiovascular Society class for angina from 2.0 +/- 1.3 to 1.3 +/- 1.2 (P = 0.08). Complete right bundle branch block developed in six patients, temporary complete atrioventricular block in three, and persistent block requiring permanent pacing in one. No flow in the distal left anterior descending coronary artery (presumably due to spilling of alcohol) was seen in one (with development of a small antero-apical infarction) and ventricular fibrillation 2 hours post-procedure in one. None of the patients died. CONCLUSION PTSMA provided a substantial reduction in left ventricular outflow gradient associated with an improvement in symptomatology. Serious complications are not uncommon. Long-term follow-up is unknown.