Miracle-dz/UniFAR-FTUs huggingface dataset:
Women Deprivation Index and Family Planning Utilisation in Urban Geography of West African Countries

Inequalities in health care utilisation and outcomes vary significantly across geographies. Though available evidence suggests disparity in contraceptive uptake in favour of urban compared with rural geographies, there are unassessed nuances among women in urban communities. This study examines some of these disparities within the context of socioeconomic deprivations and family planning utilisation among urban women in West Africa. A secondary analysis of the most recent Demographic and Health Survey dataset of five selected West African countries was conducted, using pooled data of 21,641 women aged 15–49 years. Associations between family planning utilisation and women's deprivation status were investigated using a binary logistic regression model. The findings show that more than one-quarter of the women were severely deprived across the countries except Senegal (17.4%), and the severely deprived consistently have relatively low contraceptive prevalence rates (CPR) (16.0–24.3%) compared with women with no/low deprivation across the countries except Senegal (39.8%). The results for long-acting reversible contraceptives (LARC) were not consistent across the five countries: whereas, LARC utilisation was lower among severely deprived women in Nigeria (9.1%), Guinea (9.6%), and Mali (19.3%), utilisation was similar across the deprivation groups in Benin and Senegal. In the multivariable analyses, the log-odds of modern contraceptive utilisation decreases by 0.27 among the moderately deprived (ß = −0.27, SE = 0.05, p < 0.01) and by 0.75 among the severely deprived women (ß = −0.75, SE = 0.05, p < 0.01) compared with those with no/low deprivation, with variations across the countries. Similarly, the log-odds of LARC utilisation decreases by 0.44–0.72 among the severely deprived women compared with those with no/low deprivation across the countries except Senegal. This study concluded that family planning intervention programmes and policies need to underscore the deprivation context of urban geographies, particularly among women living in informal settlements.
Diversity of Indian Barnacles in Marine Provinces and Ecoregions of the Indian Ocean

The present study is the first completed and taxonomically validated literature review of the biodiversity of barnacles (Cirripedia) in India. A total of 144 species in 75 genera and 19 families have been recorded in India. The highest number of species has been recorded from the Bay of Bengal province, located on the eastern side of the Indian Peninsula, comprising the Eastern India ecoregion (76 species) and Northern Bay of Bengal ecoregion (34 species). The West and South India Shelf province has fewer species (Western India ecoregion: 29 species; South India and Sri Lanka ecoregion: 40 species; and Maldives ecoregion: 10 species) compared to the Bay of Bengal province. The Andaman province is composed of the Andaman and Nicobar Islands, and contains 65 species. Most of the coral-associated barnacles (family Pyrgomatidae) have been recorded in the corals reefs of the Andaman and Nicobar Islands (7 species), Eastern India (6 species), and Northern Bay of Bengal ecoregions (5 species). Sponge-associated barnacles (mostly in the subfamily Acastinae) were recorded in the Eastern India ecoregion, Southern India and Sri Lanka, and Andaman and Nicobar Islands ecoregions. Deepwater species were recorded the most extensively in the Andaman and Nicobar Islands ecoregion (21 species), followed by the South India and Sri Lanka ecoregion (9 species) and Eastern India ecoregion (7 species). Six Atlantic/boreal cold water species previously reported in India were removed due to incorrect identification, and some incorrectly identified species were validated and corrected.
Early Effects of Passive Leg-Raising Test, Fluid Challenge, and Norepinephrine on Cerebral Autoregulation and Oxygenation in COVID-19 Critically Ill Patients

Background: Coronavirus disease 2019 (COVID-19) patients are at high risk of neurological complications consequent to several factors including persistent hypotension. There is a paucity of data on the effects of therapeutic interventions designed to optimize systemic hemodynamics on cerebral autoregulation (CA) in this group of patients. Methods: Single-center, observational prospective study conducted at San Martino Policlinico Hospital, Genoa, Italy, from October 1 to December 15, 2020. Mechanically ventilated COVID-19 patients, who had at least one episode of hypotension and received a passive leg raising (PLR) test, were included. They were then treated with fluid challenge (FC) and/or norepinephrine (NE), according to patients' clinical conditions, at different moments. The primary outcome was to assess the early effects of PLR test and of FC and NE [when clinically indicated to maintain adequate mean arterial pressure (MAP)] on CA (CA index) measured by transcranial Doppler (TCD). Secondary outcomes were to evaluate the effects of PLR test, FC, and NE on systemic hemodynamic variables, cerebral oxygenation (rSo2), and non-invasive intracranial pressure (nICP). Results: Twenty-three patients were included and underwent PLR test. Of these, 22 patients received FC and 14 were treated with NE. The median age was 62 years (interquartile range = 57–68.5 years), and 78% were male. PLR test led to a low CA index [58% (44–76.3%)]. FC and NE administration resulted in a CA index of 90.8% (74.2–100%) and 100% (100–100%), respectively. After PLR test, nICP based on pulsatility index and nICP based on flow velocity diastolic formula was increased [18.6 (17.7–19.6) vs. 19.3 (18.2–19.8) mm Hg, p = 0.009, and 12.9 (8.5–18) vs. 15 (10.5–19.7) mm Hg, p = 0.001, respectively]. PLR test, FC, and NE resulted in a significant increase in MAP and rSo2. Conclusions: In mechanically ventilated severe COVID-19 patients, PLR test adversely affects CA. An individualized strategy aimed at assessing both the hemodynamic and cerebral needs is warranted in patients at high risk of neurological complications.
Rail Transport Delay and Its Effects on the Perceived Importance of a Real-Time Information

Various psychological feelings that commuters might experience due to the shortcomings of a public transport are a major concern for transport policy makers. Those shortcomings would be translated into various negative psychological feelings, which would consequently tarnish the perceived quality of the public transport system in terms of its characteristics, e.g., the perceived quality of the passengers' information system (PIS). A delay has often been defined as the difference between the real arrival of a transport and the scheduled arrival of based on the PIS. The main question this study seeks to answer is how passengers view the PIS while undergoing various psychological negative impacts due to delay? This is especially important when the PIS is not precise. Previous studies on the importance of real-time information mainly focus on the impact of PIS on the satisfaction of commuters, or the reliability of the public transport. However, they rarely consider the negative psychological impacts that delays might have on commuters, and how those negative feelings might be aggravated by providing inaccurate information for the commuters. The proposed study is based on completed questionnaires by 396 passengers waiting for a rail transport in Malaysia; the rail transport was experiencing frequent long delays due to various mechanical malfunctions. In addition, the PIS provided for the passengers were mainly imprecise, and was updated regularly. The relationship between various considered variables, and a related latent factor, were formed by means of factor analysis. The results of internal consistency and validity highlight acceptable factors to be considered for a structural equation modeling (SEM) model. Three latent factors were found to impact the latent factor of PIS. For instance, it was found that the relationship between motion sickness factor and the response of PIS is not by a direct relationship between those two factors, but through the mediation of a latent physiological factor. On the other hand, the impact of the psychological feelings of the commuter by PIS is higher than its physiological effects. The results of this study have an important managerial implication for policy makers that even if the delay is inevitable, an accurate PIS could be provided to reduce the associated negative feelings of delay. Extensive discussion has been had about identification of a best fit model and process of model's parameters' estimation.
Omics Approaches to Study Formation and Function of Human Placental Syncytiotrophoblast

Proper development of the placenta is vital for pregnancy success. The placenta regulates exchange of nutrients and gases between maternal and fetal blood and produces hormones essential to maintain pregnancy. The placental cell lineage primarily responsible for performing these functions is a multinucleated entity called syncytiotrophoblast. Syncytiotrophoblast is continuously replenished throughout pregnancy by fusion of underlying progenitor cells called cytotrophoblasts. Dysregulated syncytiotrophoblast formation disrupts the integrity of the placental exchange surface, which can be detrimental to maternal and fetal health. Moreover, various factors produced by syncytiotrophoblast enter into maternal circulation, where they profoundly impact maternal physiology and are promising diagnostic indicators of pregnancy health. Despite the multifunctional importance of syncytiotrophoblast for pregnancy success, there is still much to learn about how its formation is regulated in normal and diseased states. ‘Omics’ approaches are gaining traction in many fields to provide a more holistic perspective of cell, tissue, and organ function. Herein, we review human syncytiotrophoblast development and current model systems used for its study, discuss how ‘omics’ strategies have been used to provide multidimensional insights into its formation and function, and highlight limitations of current platforms as well as consider future avenues for exploration.
The Relationship Between Decisional Regret and Well-Being in Patients With and Without Depressive Disorders: Mediating Role of Shared Decision-Making

Background: The objectives of this study were two-fold: (1) to assess the relationship between patients' decisional regret and their well-being and (2) to examine the mediated effect of shared decision-making (SDM) on this relationship. Methods: A cross-sectional survey was conducted in five cities in Southern China. Patients were asked to fill out questionnaires assessing their decisional regret, SDM, subjective well-being, and depressive status. Mediation analysis was used to investigate the effect of SDM on the relationship between patients' decisional regret and their subjective well-being. Results: The findings showed significant direct negative effects of decisional regret on subjective well-being and SDM. For non-depressive patients, SDM exerted a significant and indirect effect on reducing the negative influence of decisional regret on subjective well-being. Conclusions: Findings suggest that implementation of SDM can decrease patients' decisional regret and improve their well-being; however, there is a need to examine their depressive status as part of routine healthcare.
Notch1 and Galectin-3 Modulate Cortical Reactive Astrocyte Response After Brain Injury

After a brain lesion, highly specialized cortical astrocytes react, supporting the closure or replacement of the damaged tissue, but fail to regulate neural plasticity. Growing evidence indicates that repair response leads astrocytes to reprogram, acquiring a partially restricted regenerative phenotype in vivo and neural stem cells (NSC) hallmarks in vitro. However, the molecular factors involved in astrocyte reactivity, the reparative response, and their relation to adult neurogenesis are poorly understood and remain an area of intense investigation in regenerative medicine. In this context, we addressed the role of Notch1 signaling and the effect of Galectin-3 (Gal3) as underlying molecular candidates involved in cortical astrocyte response to injury. Notch signaling is part of a specific neurogenic microenvironment that maintains NSC and neural progenitors, and Gal3 has a preferential spatial distribution across the cortex and has a central role in the proliferative capacity of reactive astrocytes. We report that in vitro scratch-reactivated cortical astrocytes from C57Bl/6J neonatal mice present nuclear Notch1 intracellular domain (NICD1), indicating Notch1 activation. Colocalization analysis revealed a subpopulation of reactive astrocytes at the lesion border with colocalized NICD1/Jagged1 complexes compared with astrocytes located far from the border. Moreover, we found that Gal3 increased intracellularly, in contrast to its extracellular localization in non-reactive astrocytes, and NICD1/Gal3 pattern distribution shifted from diffuse to vesicular upon astrocyte reactivation. In vitro, Gal3–/– reactive astrocytes showed abolished Notch1 signaling at the lesion core. Notch1 receptor, its ligands (Jagged1 and Delta-like1), and Hes5 target gene were upregulated in C57Bl/6J reactive astrocytes, but not in Gal3–/– reactive astrocytes. Finally, we report that Gal3–/– mice submitted to a traumatic brain injury model in the somatosensory cortex presented a disrupted response characterized by the reduced number of GFAP reactive astrocytes, with smaller cell body perimeter and decreased NICD1 presence at the lesion core. These results suggest that Gal3 might be essential to the proper activation of Notch signaling, facilitating the cleavage of Notch1 and nuclear translocation of NICD1 into the nucleus of reactive cortical astrocytes. Additionally, we hypothesize that reactive astrocyte response could be dependent on Notch1/Jagged1-Hes5 signaling activation following brain injury.
Leishmania Parasites Drive PD-L1 Expression in Mice and Human Neutrophils With Suppressor Capacity

Neutrophils play an important role in the outcome of leishmaniasis, contributing either to exacerbating or controlling the progression of infection, a dual effect whose underlying mechanisms are not clear. We recently reported that CD4+ and CD8+ T cells, and dendritic cells of Leishmania amazonensis-infected mice present high expression of PD-1 and PD-L1, respectively. Given that the PD-1/PD-L1 interaction may promote cellular dysfunction, and that neutrophils could interact with T cells during infection, we investigated here the levels of PD-L1 in neutrophils exposed to Leishmania parasites. We found that both, promastigotes and amastigotes of L. amazonensis induced the expression of PD-L1 in the human and murine neutrophils that internalized these parasites in vitro. PD-L1-expressing neutrophils were also observed in the ear lesions and the draining lymph nodes of L. amazonensis-infected mice, assessed through cell cytometry and intravital microscopy. Moreover, expression of PD-L1 progressively increased in neutrophils from ear lesions as the disease evolved to the chronic phase. Co-culture of infected neutrophils with in vitro activated CD8+ T cells inhibits IFN-γ production by a mechanism dependent on PD-1 and PD-L1. Importantly, we demonstrated that in vitro infection of human neutrophils by L braziliensis induced PD-L1+ expression and also PD-L1+ neutrophils were detected in the lesions of patients with cutaneous leishmaniasis. Taken together, these findings suggest that the Leishmania parasite increases the expression of PD-L1 in neutrophils with suppressor capacity, which could favor the parasite survival through impairing the immune response.
Making Virtual Learning Environments Accessible to People with Disabilities in Universities in Uganda

Public and private universities in Uganda have been using Virtual Learning Environments (VLEs) since early 2000s to support delivery of blended learning owing to the increased uptake of technology in many aspects of life, and the benefits of blended learning/eLearning. eLearning is of particular benefit to people with disabilities, since they may find it difficult to attend classes on a university campus. Accessibility of a VLE has a strong impact on user engagement and adoption and consequently on students’ learning outcomes. Current research on use of VLEs and eLearning in general in Ugandan universities focuses on sensitization and training, the potential of social media like WhatsApp and Facebook, and required resources like Internet connectivity, and change management. In stark contrast, there is no investigation of accessibility to people with disabilities, even though about 12.4% of the population have some form of disability. This paper examines the extent to which Uganda’s policy environment promotes making eLearning accessible, reviews the accessibility of a sample of VLEs of public and private universities in Uganda, and suggests recommendations on addressing the existing accessibility gaps in policy and implementation of VLEs.
Upregulated Expression of Profilin1 on Dendritic Cells in Patients With Severe Aplastic Anemia

Severe aplastic anemia (SAA) is a life-threatening form of bone marrow failure that is associated with very high mortality. Dendritic cells (DCs) are antigen presenting cells (APCs) with powerful movement ability, which is an important factor affecting immune function. The expression of profilin1 (Pfn1) plays an important role in the regulation of cell movement ability. We detected the expression of Pfn1 mRNA in the bone marrow (BM) myeloid dendritic cells (mDCs) from patients with SAA using RT-PCR. Next, we examined Pfn1 expression on mDCs using flow cytometry (FCM). We also assessed the relationship between Pfn1 expression and cytokine levels. Our data showed increased Pfn1 mRNA expression in patients with SAA. The expression of Pfn1 in BM mDCs increased in SAA patients. The expression of Pfn1 on mDCs and cytokines (TNF-α and IFN-γ) were positively correlated in the serum of untreated patients with SAA. Taken together, we found that the expression of Pfn1 on mDCs of SAA patients increased, which may affect the function of mDCs. Profilin 1 may be involved in the immunopathogenesis of SAA.
ETS-Related Gene Expression in Healthy Femoral Arteries With Focal Calcifications

Bone development-related genes are enriched in healthy femoral arteries, which are more prone to calcification, as documented by the predominance of fibrocalcific plaques at the femoral location. We undertook a prospective histological study on the presence of calcifications in normal femoral arteries collected from donors. Since endothelial-to-mesenchymal transition (EndMT) participates in vascular remodeling, immunohistochemical (IHC) and molecular markers of EndMT and chondro-osteogenic differentiation were assessed. Transmission electron microscopy (TEM) was used to describe calcification at its inception. Two hundred and fourteen femoral arteries were enrolled. The mean age of the donors was 39.9 ± 12.9 years; male gender prevailed (M: 128). Histology showed a normal architecture; calcifications were found in 52 (24.3%) cases, without correlations with cardiovascular risk factors. Calcifications were seen on or just beneath the inner elastic lamina (IEL). At IHC, SLUG was increasingly expressed in the wall of focally calcified femoral arteries (FCFA). ETS-related gene (ERG), SLUG, CD44, and SOX-9 were positive in calcifications. RT-PCR showed increased levels of BPM-2, RUNX-2, alkaline phosphatase, and osteocalcin osteogenic transcripts and increased expression of the chondrogenic marker, SOX-9, in FCFA. TEM documented osteoblast-like cells adjacent to the IEL, releasing calcifying vesicles from the cell membrane. The vesicles were embedded in a proteoglycan-rich matrix and were entrapped in IEL fenestrations. In this study, ERG- and CD44-positive cell populations were found in the context of increased SLUG expression, thus supporting the participation of EndMT in FCFA; the increased transcript expression of osteochondrogenic markers, particularly SOX-9, reinforced the view that EndMT, osteochondrogenesis, and neoangiogenesis interact in the process of arterial calcification. Given its role as a transcription factor in the regulation of endothelial homeostasis, arterial ERG expression can be a clue of endothelial dysregulation and changes in IEL organization which can ultimately hinder calcifying vesicle diffusion through the IEL fenestrae. These results may have a broader implication for understanding arterial calcification within a disease context.
Perpetual Peacebuilding: A New Paradigm to Move Beyond the Linearity of Liberal Peacebuilding

ABSTRACT This article will introduce the ‘perpetual peacebuilding’ paradigm. Academics and policy-makers have accepted that the linearity of the liberal peace neither reflects, nor should it drive, the tumult of peacebuilding. Nevertheless, practitioners have made merely cosmetic changes to their approaches. Within the perpetual peacebuilding paradigm, peacebuilding is envisioned as an ever-developing process manifested in a series of (re-)negotiations of the social and political contract. Notions of success and failure and concepts such as ‘tracks’ and ‘peace agreements’ are abandoned, and peace is both utopian and subjective. Lastly, the peacebuilding community is called upon to display greater courage and creativity.
Preparation of Reactive and Thermal Stable Hyperbranched Graft Copolymers/ Clay Nanocomposite via ‘Living’ Free Radical Polymerization

Exfoliated poly (Chloromethyl styrene-co-styrene)-g-polyacrylonitryle/organo- modified montmorillonite [P(CMSt-co-St)-g-PAN/O-MMT] nanocomposite was synthesized through solution intercalation method by using atom transfer and nitroxide mediated radical polymerization. At first, poly (chloromethyl styrene-costyrene) copolymer was synthesized by nitroxide - mediated “living” free radical polymerization (NMRP). This chlorinated copolymer used as a macro initiators for atom transfer radical polymerization (ATRP). These macro initiators were polymerized acrylonitryle monomers in the presence of CuCl as a catalyst and the 2, 2’ – bi pyridine (bpy) as a legend in the tetrahydrofurane (THF) solvent at 90oC. Organophilic montmorillonite (O-MMT) was obtained after treating by hexadecyl trimethyl ammonium chloride salt by ion-exchange process. Finally, (PCMSt-co-PSt)-g-PMMA / O-MMT nanocomposite was prepared by solution intercalation method. The exfoliating structure of nanocomposite was probed by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
Improvement of Water-, Sulfur Dioxide-, and Dust-Resistance in Selective Catalytic Reduction of NOx with NH3 Using a Wire-Mesh Honeycomb Catalyst

A novel V2O5/WO3/TiO2/Al2O3/wire-mesh honeycomb (WMH) catalyst was prepared for selective catalytic reduction (SCR) of NOx with NH3. The resistances to H2O, SO2, and dust were investigated for the WMH catalyst, which were compared with those for ceramic honeycomb (CH) catalysts. The results showed that the WMH catalyst kept above 95% NOx conversion in the broad temperature window (250–425 °C) and provided nearly 92% NOx conversion during H2O and SO2 durability test, which might be attributed to the unique three-dimensional structure. Furthermore, the WMH catalyst could provide nearly 90% NOx conversion during 40 h dust exposure experiment owing to the little dust deposition of 2.9 g/m2, whereas the amount of dust deposited on the CH catalyst with the same cell density reached 6.7 g/m2, which resulted in a decrease of the NOx conversion from 72% to 58%.