Miracle-dz/UniFAR-FTUs huggingface dataset:
Fluorescent reporter zebrafish line for estrogenic compound screening generated using a CRISPR/Cas9-mediated knock-in system.

An increasing number of compounds in our diet and environment are being identified as estrogenic, causing serious and detrimental effects on human, animal, and ecosystem health. Time- and cost-effective biological tools to detect and screen these compounds with potential high-throughput capabilities are in ever-growing demand. We generated a knock-in zebrafish transgenic line with enhanced green fluorescent protein (EGFP) driven by the regulatory region upstream of vitellogenin 1 (vtg1), a well-studied biomarker for estrogenic exposure, using CRISPR/Cas9 technology. Exposure to 17β-estradiol (E2; 0-625 nM) starting at 4-hours post-fertilization in dechorionated embryos resulted in the significant induction of hepatic EGFP with ≥5 nM E2 as early as 3-days post-fertilization. Concentration- and time-dependent increase in the percentage of hepatic EGFP-positive larvae and extent of fluorescence expression, categorized into 3 expression levels, were observed with E2 exposure. A strong correlation between the levels of EGFP mRNA, vtg1 mRNA, and EGFP fluorescence levels were detected. Image analysis of the area and intensity of hepatic EGFP fluorescence resulted in high-fidelity quantitative measures that could be used in automated screening applications. In addition, exposure to bisphenol A (0-30 μM) resulted in quantitative responses showing promise for the use of this transgenic line to assess estrogenic activity of endocrine-disrupting chemicals. These results demonstrate that this novel knock-in zebrafish reporter allows for distinct screening of in vivo estrogenic effects, endpoints of which can be used for laboratory testing of samples for estimation of possible human and environmental risks.
BTrackS: A Low-Cost, Portable Force Plate for Objectively Measuring Balance Deficits and Fall Risk.

ow naded rom httpsournals.lw w om /om ehealthenurseonline by hD M fePH KazEoum tQ fN 4a+khEZgbsIH o4XM hC yw C X1AW YQ /IlQ rH D 3tdU 4D sBzQ 2+sQ 2N eD b09C gH 4qoN VuIJJEN 40ID W 65e6O cH vg== on 116/2019 Downloadedfromhttps://journals.lww. com/homehealthcarenurseonlinebyBhDMf5ePHKav1zEoum1tQfN4a+kJLhEZgbsIHo4XMi0hCywCX1AWnYQp/IlQrHD3ctdU4DsFBzQ2+sQ2NkeDb09CgH4qoNVu7IJJEN4g0IDW6k5e6OcHvg==on11/06/2019 FOCUS ON THERAPY
AMPKα promotes basal autophagy induction in Dictyostelium discoideum

Autophagy is a degradation process, wherein long‐lived proteins, damaged organelles, and protein aggregates are degraded to maintain cellular homeostasis. Upon starvation, 5′‐AMP‐activated protein kinase (AMPK) initiates autophagy. We show that ampkα− cells exhibit 50% reduction in pinocytosis and display defective phagocytosis. Re‐expression of AMPKα in ampkα− cells co‐localizes with red fluorescence protein‐tagged bacteria. The ampkα− cells show reduced cell survival and autophagic flux under basal and starvation conditions. Co‐immunoprecipitation studies show conservation of the AMPK–ATG1 axis in basal autophagy. Computational analyses suggest that the N‐terminal region of DdATG1 is amenable for interaction with AMPK. Furthermore, β‐actin was found to be a novel interacting partner of AMPK, attributed to the alteration in macropinocytosis and phagocytosis in the absence of AMPK. Additionally, ampkα− cells exhibit enhanced poly‐ubiquitinated protein levels and allied large ubiquitin‐positive protein aggregates. Our findings suggest that AMPK provides links among pinocytosis, phagocytosis, autophagy, and is a requisite for basal autophagy in Dictyostelium.
A non-glitch speed-up event in the Crab Pulsar

Context. The rotation history of the Crab Pulsar is well described by (1) a rotation frequency ν and a slowdown model that is specified by its first two time derivatives \hbox{$\dot \nu$} and \hbox{$\ddot \nu$}, known as the secular slowdown model; (2) occasional (once in ≈2 yr) significant and abrupt increases in the magnitude of ν and \hbox{$\dot \nu$} (occurring on timescales of minutes), known as glitches; and (3) much slower increases and decreases in ν and \hbox{$\dot \nu$} (occurring over months and years) that are an order of magnitude smaller, known as timing noise. Aims. This work reports a speed-up event in the Crab Pulsar that occurred around 2015 February that is distinct from glitches and timing noise. Methods. Monthly ν s and \hbox{$\dot \nu$}s of the Crab Pulsar, obtained at radio frequencies and published by Jodrell Bank Observatory (JBO), are used to demonstrate the speed-up event. Monthly arrival times of the Crab Pulsar’s pulse, also published by JBO, combined with X-ray data from the RXTE, Swift , and NuSTAR observatories are used to verify the result. Results. The speed-up event is caused by a persistent increase in \hbox{$\dot \nu$}, which results in a monotonic increase in ν . Over the last ≈550 days, ν has increased monotonically by an amount that is ≈10 times larger than the timing noise level. Conclusions. This is a unique event in the Crab Pulsar. This is probably due to a small increase in the Crab Pulsar’s internal temperature. In its absence, the next large glitch in the Crab Pulsar is expected to occur around 2019 March. However, this event could have an important bearing on its occurrence.
Testbeam results of irradiated ams H18 HV-CMOS pixel sensor prototypes

HV-CMOS pixel sensors are a promising option for the tracker upgrade of the ATLAS experiment at the LHC, as well as for other future tracking applications in which large areas are to be instrumented with radiation-tolerant silicon pixel sensors. We present results of testbeam characterisations of the 4th generation of Capacitively Coupled Pixel Detectors (CCPDv4) produced with the ams H18 HV-CMOS process that have been irradiated with different particles (reactor neutrons and 18 MeV protons) to fluences between 1× 1014 and 5× 1015 1−MeV− neq. The sensors were glued to ATLAS FE-I4 pixel readout chips and measured at the CERN SPS H8 beamline using the FE-I4 beam telescope. Results for all fluences are very encouraging with all hit efficiencies being better than 97% for bias voltages of 85 V. The sample irradiated to a fluence of 1× 1015 neq—a relevant value for a large volume of the upgraded tracker—exhibited 99.7% average hit efficiency. The results give strong evidence for the radiation tolerance of HV-CMOS sensors and their suitability as sensors for the experimental HL-LHC upgrades and future large-area silicon-based tracking detectors in high-radiation environments.
The influence of sleep quality and circadian preferences on upper extremity rehabilitation in stroke patients after constraint-induced movement therapy

Our aim was to explore the influence of sleep and circadian preference on upper extremity (UE) rehabilitation in stroke patients after constraint-induced movement therapy (CIMT) in a cross-sectional retrospective observational study. Forty-three patients were selected to complete questionnaires on circadian preference, sleep quality, excessive daytime sleepiness, and risk of obstructive sleep apnea. They had undergone a 10-day standard CIMT program without medical complications and with normal to minimal cognitive dysfunction. All pre- and postrehabilitation scores (patient perception of the quantity and quality of use of the affected UE and self-quantification of motor ability) were analyzed retrospectively. All patients had improved perception of the quantity and quality of use of the affected UE and self-quantified motor ability. Patients with an evening-type chronotype demonstrated less improvement than those with morning and intermediate types. In addition, patients with poor sleep quality showed less improvement in functional ability than those with good sleep quality. Circadian preferences and sleep quality impacted the improvements in motor performance of patients with stroke after CIMT rehabilitation. This is the first study to suggest that rehabilitation sessions must respect the circadian preferences of patients and that sleep quality can affect outcomes. Future studies should investigate the relationship and mechanisms between circadian preference and poor sleep quality and rehabilitation outcomes on a larger scale.
Modular Strategies to Build Cell-Free and Cell-Laden Scaffolds towards Bioengineered Tissues and Organs

Engineering three-dimensional (3D) scaffolds for functional tissue and organ regeneration is a major challenge of the tissue engineering (TE) community. Great progress has been made in developing scaffolds to support cells in 3D, and to date, several implantable scaffolds are available for treating damaged and dysfunctional tissues, such as bone, osteochondral, cardiac and nerve. However, recapitulating the complex extracellular matrix (ECM) functions of native tissues is far from being achieved in synthetic scaffolds. Modular TE is an intriguing approach that aims to design and fabricate ECM-mimicking scaffolds by the bottom-up assembly of building blocks with specific composition, morphology and structural properties. This review provides an overview of the main strategies to build synthetic TE scaffolds through bioactive modules assembly and classifies them into two distinct schemes based on microparticles (µPs) or patterned layers. The µPs-based processes section starts describing novel techniques for creating polymeric µPs with desired composition, morphology, size and shape. Later, the discussion focuses on µPs-based scaffolds design principles and processes. In particular, starting from random µPs assembly, we will move to advanced µPs structuring processes, focusing our attention on technological and engineering aspects related to cell-free and cell-laden strategies. The second part of this review article illustrates layer-by-layer modular scaffolds fabrication based on discontinuous, where layers’ fabrication and assembly are split, and continuous processes.
High Potency of SN-38-Loaded Bovine Serum Albumin Nanoparticles Against Triple-Negative Breast Cancer

Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer with a worse prognosis than other types. There are currently no specific approved treatments for TNBC. Albumin is a promising biomimetic material that may be fabricated into nanoparticles to possibly exert passive effects on targeted tumors. Irinotecan has been extensively used in clinical settings, although a high dosage is required due to its low efficiency of conversion into the active metabolite SN-38, also known as 7-ethyl-10-hydroxy-camptothecin. The aim of this work was to optimize SN-38-loaded bovine serum albumin nanoparticles (sBSANPs) and evaluate their potency against TNBC. The sBSANPs were characterized by a small size of about 134–264 nm, a negative charge of −37 to −40 mV, an entrapment efficiency of 59–71%, and a particle yield of 65–86%. The cytotoxicity assays using sBSANPs showed a higher potency specifically against both MDA-MB-468 and MDA-MB-231 cells (ER−, PR−, HER2−) compared to MCF-7 (ER+, PR+, HER2−), and exhibited an extremely low IC50 at the nanomolar levels (2.01–6.82 nM). The release profiles indicated that SN-38 presented an initial burst release within 12 h, and sBSANPs had a slow release pattern. Flow cytometry results showed that the fluorescence intensity of sBSANPs was significantly higher than that of the control group. The confocal images also confirmed that sBSANPs were taken up by MDA-MB-468 cells. Moreover, we found that a larger BSANP size resulted in an increased hemolytic effect. In vivo animal studies demonstrated that loading of SN-38 into bovine serum albumin nanoparticles could minimize the initial concentration without extending the elimination half-life, but significantly minimized the Cmax (p < 0.001) as compared with irinotecan treatment.
Molecular Detection and Predominance of Human Torque Teno Virus in Children’s with acute hepatitis and Environmental Waters

Introduction: Epidemiological studies have shown that TTV is described worldwide in various populations. The prevalence of TTV viremia in healthy adults of developed countries is in the range of 1-34%. Prevalence reported from third world countries was found to be higher, typically 40-70%. In people who have received multiple blood transfusion; the virus is almost universally present with more than one subtype in each individual. Objectives: This study was carried out to determine the incidence of TTV infection among children's, wastewater, and drinking water, also genotyping of detected TTV strains circulating in the studied samples collected from Cairo. Subjects and Methods: stool samples were collected from 152 Children’s with age range from 6 months to 12 years. Also, 45 wastewater samples and 45 drinking water samples were collected monthly from inlet and outlet from wastewater treatment plant (Zenin) and from El-Giza drinking water treatment plant. Collected samples were submitted to seminested PCR for amplification of 3' non translated region of TTV. The expected fragment sizes of PCR products were 390 bp and 271 bp for the first and second round of PCR, respectively. PCR products, of 2 round of some positive samples, were purified for nucleotide sequence analysis in both directions. Fragments nucleotide sequences were compared to sequences derived from the corresponding TTV genome of the same regions deposited in the Gene Bank. Results and Conclusion: Obtained data showed that the incidence of TTV in children's stool samples was 59.2% (90/152), and in sewage samples was 64.4% (29/45), while it was 17.8% (8/45) in drinking water samples. Statistical analysis indicated that there no significant difference in TTV infection between male and female in infant and early childhood age groups. The phylogenic tree of positive samples confirmed that the isolated virus sequencing was 100% of nucleotide identity to TTV isolate isolated in Germany in 2001 and deposited in the GeneBank with accession no. AF435014, and the sequence of TTV isolated in the present study belong to TTV genotype 28. [Shoeib, A.R. S, El-Esnawy, N.A and Zarouk, A. W. Molecular Detection and Predominance of Human Torque Teno Virus in Children’s with acute hepatitis and Environmental Waters. Life Science Journal,. 2011;8(3):165-172] (ISSN: 1097-8135). http://www.lifesciencesite. com.
CONSTANT QUALITY HOUSE PRICES IN AN AUSTRALIAN CONTEXT A CASE STUDY OF PORT PIRIE, SOUTH AUSTRALIA

This paper examines the relationship between median and mean house prices, which are commonly misconstrued in the popular press to mean the asset price, and constant-quality house prices as measured by hedonic equations and repeat sales. Median and mean house prices for relatively homogeneous sub markets are also examined, to determine whether there is potential to gauge asset price movements without the time and effort involved in constructing repeat sale and hedonic price indices. The case chosen for examination is Port Pirie, in South Australia. This small city has had large apparent price increases over a period of time in which real prices of housing in many Australian regional areas have apparently fallen. The sales data is divided into more homogeneous submarkets, to ascertain whether mean or median closely reflect the asset prices in these sub markets. Hedonic price and repeat sales indices are then constructed so that the usefulness of mean and median prices as housing price movement indicators in Port Pirie can be ascertained.
The I Online - Identity Conceptualisation in Social Media and the Implications for Online Marketing Communications

Purpose: To determine which, why and how social media tools are used by young adults to conceptualise identity/identities online, the extent of such activity and the implications for online marketing communications. Literature review: New internet technologies have altered the way brands and consumers interact with power shifting from organisations to consumers’. Social media allows users the possibility of creating and maintaining multiple identities making the task of locating them increasingly difficult for marketers. In reality we adopt several aspects of self but the extent of this behaviour online is little researched. For marketers to understand and successfully target their audience it is necessary to also understand individual uses and gratifications of online interaction. Methodology: The research undertakes a qualitative data collection. Primarily a pilot study was undertaken. Thereafter primary data for the main study was collected through in-depth interviews and secondary data was collected from academic literature and reports. Findings: The social media tools adopted are motivated by respondents private and career interests. Self representation is apparent and differs between sites regarding what part of their identity to disclose and conceal. Status, empowerment and reputation are important motivators for the respondents’ social media interaction, through which we also discover their central uses and gratifications of social media. In terms of brand interaction we discover various ways in which twoway online communication between both users and brands is greatly appreciated. Conclusion: Through social media tools, young adults choose to disclose different parts of their self depending on the uses and gratifications of the site. Both communication among peers and direct brand communication are important when adopting brands. Although they are individualistic, peergroup support does play a role in their online interactions. Motivations for young adults online are immediacy, empowerment and the creation and management of identity. For marketers the challenge lies in regaining partial control by motivating consumer interaction.
course : a DEDIPAC-study

Background: Ecological models are currently the most used approaches to classify and conceptualise determinants of sedentary behaviour, but these approaches are limited in their ability to capture the complexity of and interplay between determinants. The aim of the project described here was to develop a transdisciplinary dynamic framework, grounded in a system-based approach, for research on determinants of sedentary behaviour across the life span and intervention and policy planning and evaluation. Methods: A comprehensive concept mapping approach was used to develop the Systems Of Sedentary behaviours (SOS) framework, involving four main phases: (1) preparation, (2) generation of statements, (3) structuring (sorting and ranking), and (4) analysis and interpretation. The first two phases were undertaken between December 2013 and February 2015 by the DEDIPAC KH team (DEterminants of DIet and Physical Activity Knowledge Hub). The last two phases were completed during a two-day consensus meeting in June 2015. (Continued on next page) * Correspondence: Sebastien.Chastin@gcu.ac. uk Institute for Applied Health Research, School of Health and Life Science, Glasgow Caledonian University, Glasgow G4 0BA, UK Full list of author information is available at the end of the article © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Chastin et al. International Journal of Behavioral Nutrition and Physical Activity
Molecular cloning , sequence characterization , and gene expression profile of a novel water buffalo ( Bubalus bubalis ) gene : Na + , K +-ATPase β 2-subunit ( ATP 1 B 2 )

The Na+, K+-ATPase is a transmembrane carrier protein which plays an important role in Na-K transport and energy metabolism. The aim of this study was to obtain the full-length coding sequence (CDS) of water buffalo Na+, K+-ATPase β2-subunit (ATP1B2) using RT-PCR and to investigate the characterizations of its sequence and tissue expression patterns. Sequence analysis revealed that the CDS of water buffalo ATP1B2 encodes an enzyme of 290 amino acid residues with a deduced molecular weight of 33.39 KDa and a PI of 8.37. Water buffalo ATP1B2 was presumed to have a signal peptide, a strong hydrophobic region and to exert its function in the plasma membrane with high reliability. In addition, water buffalo ATP1B2 has a conserved Na+, K+-ATPase β domain which belongs to Na+, K+-ATPase superfamily. The sequence of water buffalo ATP1B2 gene shares 97.6, 97.4, 92.2, 93.2, 89.9, 93.2, 89.5 and 62.9 % identify with its homologous sequence of cattle, sheep, dog, human, mouse, baboon, rat and African clawed frog, respectively. Phylogenetic tree analysis based on the CDS of ATP1B2 gene showed that water buffalo has a closer genetic relationship with cattle than with other species. The ATP1B2 gene was widely expressed in the tissues examined, being high in the pituitary gland and brain, moderate in the muscle, spleen, liver, mammary gland, kidney and rumen, weak in the heart, small intestines and skin, and not expressed in the lung and adipose tissue. This study will establish a foundation for further insights into this novel water buffalo gene. *These authors contributed equally to this study. Open Access Archiv Tierzucht 57 (2014) 15, 1-12 Received: 26 July 2013 doi: 10.7482/0003-9438-57-015 Accepted: 30 April 2014 Online: 30 June 2014 Corresponding author: Yongwang Miao, email: yongwangmiao999@163.com or miaoyw1@ynau.edu. cn Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, China © 2014 by the authors; licensee Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany. This is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution 3.0 License (http://creativecommons.org/licenses/by/3.0/). Song et al.: Cloning, characterization and expression profile of ATP1B2 2
Measurement of the b-hadron production cross section using þÿ �

Aaltonen , T , Mehtälä , P , Orava , R , Mäki , T , Saarikko , H , Österberg , K & CDF Collaboration 2009 , ' Measurement of the b-hadron production cross section using decays þÿ t o 1⁄4 D 0 X f i n a l s t a t e s i n p p c o l l i s i o n s a t " s = 1 . 9 6 T e V ' P h y s i c a l R e v i e w D : P a r t i c l e s , Fields, Gravitation and Cosmology , vol. 79 , no. 9 , pp. 092003 . https://doi.org/10.1103/PhysRevD.79. 092003
The Dipterose of Black Soldier Fly (Hermetia illucens) Induces Innate Immune Response through Toll-Like Receptor Pathway in Mouse Macrophage RAW264.7 Cells

In our study, a novel bioactive polysaccharide was identified in the larvae of the black soldier fly (BSF) (Hermetia illucens) as a molecule that activates the mammalian innate immune response. We attempted to isolate this molecule, which was named dipterose-BSF, by gel-filtration and anion-exchange chromatography, followed by nitric oxide (NO) production in mouse RAW264.7 macrophage cells as a marker of immunomodulatory activity. Dipterose-BSF had an average molecular weight of 1.47 × 105 and consisted of ten monosaccharides. Furthermore, in vitro assays demonstrated that dipterose-BSF enhanced the expression of proinflammatory cytokines and interferon β (IFNβ) in RAW264.7 cells. The inhibition of Toll-like receptor 2 (TLR2) and 4 (TLR4) significantly attenuated NO production by dipterose-BSF, indicating that dipterose-BSF stimulates the induction of various cytokines in macrophages via the TLR signaling pathway. This observation was analogous with the activation of nuclear factor kappa B in RAW264.7 cells after exposure to dipterose-BSF. Our results suggest that dipterose-BSF has immunomodulatory potential through activating the host innate immune system, which allows it to be a novel immunomodulator for implementation as a functional food supplement in poultry, livestock, and farmed fish.