Miracle-dz/UniFAR-FTUs huggingface dataset:
Challenges and Opportunities of Providing Pediatric Funeral Services: A National Survey of Funeral Professionals

Background: Funeral professionals offer a unique opportunity to support, educate, and advocate for grieving families after the death of a child. From the initial point of contact to the burial, funeral professionals shape the final moments and memories of a family and a community. And yet, little is known about the needs of funeral professionals to provide high quality services when a child dies. Objectives: To conduct a needs assessment among funeral professionals in conducting funeral services for children. Methods: Active National Funeral Directors Association members were surveyed on their experience and needs in providing pediatric funeral services. The survey contained 41 questions about experience, skills, and knowledge in providing pediatric funerals. The survey included multiple choice, yes/no, and open-ended questions. Descriptive statistics were generated from the survey items and comments/open-ended questions were coded and assessed for themes. Results: More than 200 responses were received. The respondents conducted 43 pediatric funeral services in a 5-year period (approx. 8 services/year). The top 5 self-identified training needs included grieving parents, grieving siblings, memorials, comforting the community, and outreach. Themes from the open-ended questions were (a) maintaining professionalism, (b) managing emotions and experiences, (c) assessing family dynamics, (d) managing professional skills, and (e) building collaborative networks. Conclusions: Specific training needs were identified that will inform the development of targeted training for funeral professionals to improve their skills and knowledge of pediatric funeral services.
Low-dose theophylline in addition to ICS therapy in COPD patients: A systematic review and meta-analysis

Background A synergism has been reported between theophylline and corticosteroids, wherein theophylline increases and restores the anti-inflammatory effect of inhaled corticosteroids (ICS) by enhancing histone deacetylase-2 (HDAC) activity. Several studies have explored the efficacy of low-dose theophylline plus ICS therapy on chronic obstructive pulmonary disease (COPD) but the results are discrepant. Method We conducted searches in electronic database such as PubMed, Web Of Science, Cochrane Library, and Embase to find out original studies. Stata/SE 15.0 was used to perform all data analysis. Result A total of 47,556 participants from 7 studies were included in our analysis and the sample size of each study varied from 24 to 10,816. Theophylline as an add-on therapy to ICS was not associated with the reduction of COPD exacerbations (HR: 1.08, 95% CI: 0.97 to 1.19, I2 = 95.2%). Instead, the theophylline group demonstrated a higher hospitalization rate (HR: 1.12, 95% CI: 1.10 to 1.15, I2 = 20.4%) and mortality (HR: 1.19, 95% CI: 1.14 to 1.25, I2 = 0%). Further, the anti-inflammatory effect of low-dose theophylline as an adjunct to ICS on COPD was controversial. Besides, the theophylline group showed significant improvement in lung function compared with the non-theophylline group. Conclusion Based on current evidence, low-dose theophylline as add-on therapy to ICS did not reduce the exacerbation rate. Instead, the hospitalization rate and mortality increased with theophylline. Thus, we do not recommend adding low-dose theophylline to ICS therapy in COPD patients. Trial registration PROSPERO Registration CRD42021224952.
Investigation of mass-scale drift effects in the milli-mass range: Influence on high mass resolution mode multicollector-inductively coupled plasma mass spectrometer isotope ratio measurements.

The consequences, possible origins, and prevention of mass-scale drifts in the high mass resolution mode (HR, M/ΔM ≈ 8000) under constant conditions were investigated and simulated in case of a multicollector-inductively coupled plasma mass spectrometer (MC-ICP-MS) using silicon enriched in 28 Si as the main element in this survey. A drifting mass scale strongly impairs the precise and accurate determination of isotope ratios, depending especially on the peak/plateau width. For example, 29 Si+ in Si highly enriched in 28 Si has an extremely small mass plateau width of ΔM ≤ 4 × 10-3 u, compare ΔM(56 Fe+ ) ≈ 18 × 10-3 u, which is to our knowledge one of the smallest plateaus routinely investigated in isotope ratio measurements, thus requiring extreme stability. During warm-up of the double-focusing sector field mass spectrometer, a mass drift up to ΔM/Δt ≥ 0.006 u/hr has been observed. Long-term studies on mass scale stability and simulations concerning fluctuations of the magnetic field B, acceleration voltage Uacc and ESA voltage UESA are reported. A change of one of these quantities of 0.01% induces changes of the mass scale of 6 × 10-3 u, 3 × 10-3 u, and 1 × 10-3 u in the case of B, Uacc , and UESA , respectively. After identifying electrical charging/discharging effects in the mass spectrometer affecting the mass scale stability, the instrument was completely dismantled and carefully reinstalled. Additional stability tests using silicon, strontium, and lead finally yielded a mass drift of ΔM/Δt ≤ 0.001 u/8 h in the case of silicon. This enhanced stability guarantees measurements of isotope ratios on smallest plateaus with lowest uncertainty. The importance of a stable mass scale is pointed out and the relevant quantities of a typical magnetic sector field mass spectrometer are discussed.
Orexin a levels of adolescents with major depressive disorder

Abstract Objective This study examines orexin A levels in adolescents with major depressive disorder (MDD). Methods Serum orexin A levels of adolescents with MDD (n = 40) were compared to healthy controls (n = 38) using ANCOVA test. In addition, the relationship between orexin A levels and MDD symptom severity (i.e., child depression inventory) was investigated in the MDD group using correlation and linear regression analyses. Results Orexin A levels of the subjects with MDD were similar to controls while controlling for age, gender, body mass index, and anxiety levels of the subjects. In addition, correlation and regression analyses did not reveal any relationship between orexin A and MDD symptoms. Discussion Adolescent MDD is not associated with orexin A according to the findings of this study. Future studies considering the effect of stress on this relationship would improve our understanding of this issue. Key Points Adult studies exploring the relationship between orexin A and major depressive disorder reported contradictory findings. This study showed no relationship between serum orexin A levels and depressive symptom severity among adolescents with major depressive disorder. Orexin A levels of the subjects with major depressive disorder are not significantly different from healthy adolescents.
CDCA2 promotes tumorigenesis and induces radioresistance in oesophageal squamous cell carcinoma cells

Cell division cycle-associated 2 (CDCA2) overexpression has been demonstrated to serve a significant role in tumorigenesis in certain types of cancer. Nevertheless, its role in tumour proliferation and radioresistance in oesophageal squamous cell carcinoma (ESCC) remains to be elucidated. Thus, the present study aimed to elucidate these roles. Data were downloaded from The Cancer Genome Atlas (TCGA) to compare the gene expression profiles. The expression of CDCA2 was higher in ESCC tissues compared with normal tissues. Gene set enrichment analysis was performed based on the ESCC cohorts in TCGA database. This demonstrated that higher expression of CDCA2 was significantly associated with the expression of related components of the cell cycle phase transition and G2/M phase transition pathways. Collectively, the results revealed that CDCA2 could serve as an underlying target to regulate tumour growth and radioresistance among patients with ESCC.
Seeking tirelessly for better health and life conditions for the child with myelomeningocele*

Objective: to understand the experience of families in the care of children with myelomeningocele. Method: a qualitative research study, which adopted Symbolic Interactionism and Grounded Theory as its theoretical and methodological framework, and the semi-structured interview as a data collection instrument. Twenty-eight participants from thirteen families living in a municipality in the inland of So Paulo took part in the study. Results: the family tirelessly seeks better health and life conditions for the child with myelomeningocele; aiming at the childs autonomy, it is mobilized to the necessary treatments, to learn the care, to adapt the routine, as well as to supply for all the childs needs so that it reaches potential development, looking for a less dependent future with inclusion and social ascension. Conclusion: family nursing shows potential support in the face of myelomeningocele, due to its ability to apprehend the family system, evaluate it and enable intervention proposals in the care process. In this study, rehabilitation nursing was also emphasized, since it goes together with the child and the family who experience myelomeningocele.
On the theory of metal nanoparticles based on quantum mechanical calculation.

Metal nanoparticles have attracted considerable attention owing to their unusual physical and chemical properties from those of their molecular and bulk counterparts and are fundamental to surface science applications such as catalysts, optics, photonics, sensors, and spectroscopy. Traditionally, the optical absorption spectra are derived from the collective oscillations of free electrons of conduction band in metal nanoparticles as a consequence of incident electromagnetic radiation polarizing the nanoparticles. This phenomenon, known as the localized surface plasmon resonance, is unique to metallic nanostructures and has been modelled by Gustav Mei in 1908 based on the Maxwell’s equations. It is the most-cited scientific paper of 20th century and this classical approach is still used widely. However, the theory cannot account for quantum confinement effects of the electronic structure, the fundamental physical properties of metal nanoparticles. More satisfying treatment of photons interacting with metal nanoparticles is by a quantum theory approach. When UV-visible light impinging on a metal nanoparticle, occupied ground-state conduction electrons absorb photons and excite to higher unoccupied higher energy-state of the conduction band of the particle. In this development we used time-independent Schrodinger equation of the ground-state energy of Thomas-Fermi-Dirac-Weizsacker atomic model and also the density function in the final Euler-Lagrange equation was algebraically substituted with the absorption function. The total energy functional was computed numerically for isolated silver and gold nanospheres at various sizes. The electronic transitions within the conduction band are limited only by the Lagrange multiplier and the quantum number selection rules. The calculated absorption peaks fall within the experimental regimes. The results show a red-shift absorption peak increases with the increase of particle diameter corresponds to a decrease in the conduction band energy of metal nanoparticles.
Bartonella henselae Persistence within Mesenchymal Stromal Cells Enhances Endothelial Cell Activation and Infectibility That Amplifies the Angiogenic Process

Some bacterial pathogens can manipulate the angiogenic response, suppressing or inducing it for their own ends. In humans, Bartonella henselae is associated with cat-scratch disease and vasculoproliferative disorders such as bacillary angiomatosis and bacillary peliosis. Although endothelial cells (ECs) support the pathogenesis of Bartonella, the mechanisms by which Bartonella induces EC activation are not completely clear, as well as the possible contributions of other cells recruited at the site of infection. Mesenchymal stromal cells (MSCs) are endowed with angiogenic potential and play a dual role in infections, exerting antimicrobial properties but also acting as a shelter for pathogens. ABSTRACT Some bacterial pathogens can manipulate the angiogenic response, suppressing or inducing it for their own ends. In humans, Bartonella henselae is associated with cat-scratch disease and vasculoproliferative disorders such as bacillary angiomatosis and bacillary peliosis. Although endothelial cells (ECs) support the pathogenesis of B. henselae, the mechanisms by which B. henselae induces EC activation are not completely clear, as well as the possible contributions of other cells recruited at the site of infection. Mesenchymal stromal cells (MSCs) are endowed with angiogenic potential and play a dual role in infections, exerting antimicrobial properties but also acting as a shelter for pathogens. Here, we delved into the role of MSCs as a reservoir of B. henselae and modulator of EC functions. B. henselae readily infected MSCs and survived in perinuclearly bound vacuoles for up to 8 days. Infection enhanced MSC proliferation and the expression of epidermal growth factor receptor (EGFR), Toll-like receptor 2 (TLR2), and nucleotide-binding oligomerization domain-containing protein 1 (NOD1), proteins that are involved in bacterial internalization and cytokine production. Secretome analysis revealed that infected MSCs secreted higher levels of the proangiogenic factors vascular endothelial growth factor (VEGF), fibroblast growth factor 7 (FGF-7), matrix metallopeptidase 9 (MMP-9), placental growth factor (PIGF), serpin E1, thrombospondin 1 (TSP-1), urokinase-type plasminogen activator (uPA), interleukin 6 (IL-6), platelet-derived growth factor D (PDGF-D), chemokine ligand 5 (CCL5), and C-X-C motif chemokine ligand 8 (CXCL8). Supernatants from B. henselae-infected MSCs increased the susceptibility of ECs to B. henselae infection and enhanced EC proliferation, invasion, and reorganization in tube-like structures. Altogether, these results indicate MSCs as a still underestimated niche for persistent B. henselae infection and reveal MSC-EC cross talk that may contribute to exacerbate bacterium-induced angiogenesis and granuloma formation.
Mothers’ perceptions on and learning from infant and young child-feeding videos displayed in Mother and Child Health Centers in Kenya: a qualitative and quantitative approach

Abstract Objective: The objective was to explore mothers’ perceptions on educational videos on infant and young child-feeding practices, and to assess whether viewing frequencies would influence maternal knowledge, attitudes and feeding practices (KAP). Design: A set of forty-seven videos were displayed in health centres for 6 months. At 3 months, we conducted focus group discussions (FGD) with mothers and, at 6 months, administered KAP-questionnaire-based interviews to mothers. Using a quasi-experimental design, we compared groups according to video viewing frequencies. Setting: The study was conducted in a slum in Nairobi and a rural area in Machakos, Kenya. We installed TV screens in waiting rooms of six Mother and Child Health Centers, where mothers could choose to watch them. Participants: Forty-three mothers with children aged 0–48 months participated in six FGD and 547 mothers of children aged 0–23 months in KAP interviews. Results: The mothers from the FGD found the videos acceptable and beneficial. Videos enhanced mothers’ learning and empowered them to support others in learning. The KAP data showed that after adjustments, breast-feeding (P = 0·06), complementary feeding knowledge (P = 0·01), complementary feeding attitudes (P = 0·08) and hygiene knowledge and practices (P = 0·003) were better among mothers who had seen videos three to four times, or five or more times, compared with mothers who had seen the videos once or twice. Conclusions: Videos were an accepted form of education and were beneficial when watched repeatedly. The videos could be a good addition to current infant and young child-feeding education efforts in Kenya.
Bovine semen sexing: Sperm membrane proteomics as candidates for immunological selection of X‐ and Y‐chromosome‐bearing sperm

Abstract The use of sexed semen in dairy and beef farms ensures the production of animals of the desired sex, resulting in a reduction of costs and an improvement of environmental sustainability. Several methods have been developed over the years, but most of them were abandoned due to their limited efficacy. Currently, the only commercially available method for the separation of X‐ and Y‐chromosome‐bearing sperm is fluorescence‐activated cell sorting. However, this technique is expensive and has limited usefulness for the industry, considering that it cannot produce doses of sexed semen with the desired number of sperm for artificial insemination. Immunological methods have emerged as an attractive alternative to flow cytometry and proteomic knowledge of X‐ and Y‐sperm could be useful to the development of a new method. In this review, we identify the main applications of sexed semen, describe the existing methods and highlight future research opportunities in the field. We consider that immunological methods, based on sperm cell's surface proteins differentially expressed between X‐ and Y‐sperm, could be an interesting and promising approach to semen sexing.
The role of angiotensin-(1-7) on acquired platinum resistance-induced angiogenesis in non-small cell lung cancer in vitro and in vivo.

Renin-angiotensin system (RAS) signaling has been implicated in the development of cancer. The new RAS ACE2/Ang-(1-7)/Mas axis antagonizes the classical ACE/Ang II/AT1R axis. Ang-(1-7) has pleiotropic roles in lung cancer including suppressing proliferation, angiogenesis, and metastasis. This research was designed to investigate the effect of Ang-(1-7) on tumor-associated angiogenesis in DDP-resistant lung cancer cell lines. We first established acquired DDP-resistant cell lines A549 (A549-DDP) and LCC (LLC-DDP). We next performed RT-qPCR, western blot, ELISA, tube formation, microvessel density detection, immunohistochemistry, and tumor formation assays. The results showed that the mRNA and protein levels of RAS components and vascular endothelial growth factor A (VEGFa) were lessened in the A549/LLC-DDP-Ang-(1-7) group compared with the A549/LLC-DDP group. This effect could be blocked by the MAS receptor antagonist A779. The data revealed that Ang-(1-7) could perform its antiangiogenic function by PI3K/AKT and MAPK pathways. Furthermore, the impact of Ang-(1-7) on tumor-associated angiogenesis has been confirmed in lung cancer xenograft model with acquired DDP resistance. These results provide a theoretical basis for designing therapeutic strategies for targeting Ang-(1-7) in the treatment of NSCLC.
Aberrant expression of SATB2, CDX2, CDH17 and CK20 in hepatocellular carcinoma: a pathological, clinical and outcome study

Data regarding expression of intestinal markers in hepatocellular carcinoma (HCC) are limited. We determined the clinicopathological associations of cytokeratin (CK)19, a progenitor liver epithelial cell marker as well as biliary epithelial marker, and intestinal immunohistochemical markers expression in HCC and assessed their prognostic value.
Using Electronic Health Records to Mitigate Workplace Burnout Among Clinicians During the COVID-19 Pandemic: Field Study in Iran

Background The COVID-19 pandemic spread worldwide in 2020. Notably, in the countries dealing with massive casualties, clinicians have worked in new conditions characterized by a heavy workload and a high risk of being infected. The issue of clinician burnout during the pandemic has attracted considerable attention in health care research. Electronic health records (EHRs) provide health care workers with several features to meet a health system’s clinical needs. Objective We aim to examine how the use of EHR features affects the burnout of clinicians working in hospitals that have special wards for confirmed COVID-19 cases. Methods Using an online survey, we collected data from 368 physicians, physician assistants, and nurses working in six hospitals that have implemented EHRs in the city of Tehran in Iran. We used logistic regression to assess the association between burnout and awareness of EHR features, EHR system usability, concerns about COVID-19, technology solutions, hospital technology interventions, hospital preparedness, and professional efficacy adjusted for demographic and practice characteristics. Results The primary outcome of our study was self-reported burnout during the COVID-19 pandemic. Of the 368 respondents, 36% (n=134) reported having at least one symptom of burnout. Participants indicated that the leading cause of EHR-related stress is inadequate training for using technology (n=159, 43%), followed by having less face-to-face time with patients (n=140, 38%). Positive perceptions about the EHR’s ease of use were associated with lower odds of burnout symptoms. More interventions, such as clear communication of regulations; transparency in policies, expectations, and goals regarding the use of technology in the clinical workflow; and hospital preparedness to cope with the challenges of the pandemic, were associated with lower odds of burnout. Conclusions The use of EHR applications, hospital pandemic preparation programs, and transparent technology-related policies and procedures throughout the epidemic can be substantial mitigators of technology-based stress and clinician burnout. Hospitals will then be better positioned to devise or modify technology-related policies and procedures to support physicians’ and nurses’ well-being during the COVID-19 pandemic. Training programs, transparency in communications of regulations, and developing a clear channel for informing clinicians of changes in policies may help reduce burnout symptoms among physicians and nurses during a pandemic. Providing easily accessible mentorship through teleconsultation and 24-hour available information technology support may also help to mitigate the odds of burnout.
Vehicle Detection From UAV Imagery With Deep Learning: A Review

Vehicle detection from unmanned aerial vehicle (UAV) imagery is one of the most important tasks in a large number of computer vision-based applications. This crucial task needed to be done with high accuracy and speed. However, it is a very challenging task due to many characteristics related to the aerial images and the used hardware, such as different vehicle sizes, orientations, types, density, limited datasets, and inference speed. In recent years, many classical and deep-learning-based methods have been proposed in the literature to address these problems. Handed engineering- and shallow learning-based techniques suffer from poor accuracy and generalization to other complex cases. Deep-learning-based vehicle detection algorithms achieved better results due to their powerful learning ability. In this article, we provide a review on vehicle detection from UAV imagery using deep learning techniques. We start by presenting the different types of deep learning architectures, such as convolutional neural networks, recurrent neural networks, autoencoders, generative adversarial networks, and their contribution to improve the vehicle detection task. Then, we focus on investigating the different vehicle detection methods, datasets, and the encountered challenges all along with the suggested solutions. Finally, we summarize and compare the techniques used to improve vehicle detection from UAV-based images, which could be a useful aid to researchers and developers to select the most adequate method for their needs.