Miracle-dz/UniFAR-FTUs huggingface dataset:
Protein tyrosine phosphatase receptor type O (PTPRO) knockdown enhances the proliferative, invasive and angiogenic activities of trophoblast cells by suppressing ER resident protein 44 (ERp44) expression in preeclampsia

ABSTRACT Preeclampsia (PE), a pregnancy-specific syndrome, is the primary cause of maternal mortality. This work was designed to investigate the specific functions of PTPRO/ ERp44 in the biological behaviors of trophoblast cells and elucidate the underlying molecular mechanism. Constructed siRNA-PTPRO and ERp44 overexpression plasmids were transfected into HTR-8/SVneo and JEG-3 cells for further functional experiments. Subsequently, the proliferation and invasion of trophoblast cells were identified by performing CCK-8, flow cytometry and transwell assay. In addition, tube formation assay was employed to estimate the angiogenic ability of HUVECs incubated with the conditioned media (CM) of HTR-8/SVneo or JEG-3 cells. Importantly, the interaction between PTPRO and ERp44 was analyzed through Co-IP. In the current investigation, it was discovered that downregulation of PTPRO notably facilitated the proliferation and invasion of trophoblast cells and induced a stronger in vitro angiogenesis. Moreover, PTPRO interacted with ERp44 to regulate ERp44 expression. ERp44 overexpression suppressed the proliferative, invasive and angiogenic activities of trophoblast cells. As a result, functions of PTPRO knockdown in the biological behaviors of trophoblast cells were partially abrogated upon elevation of ERp44. To sum up, this current research systematically evidenced that PTPRO could regulate the biological behaviors of trophoblast cells by modulating ERp44. Findings may contribute to a novel therapeutic strategy for PE.
Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair

Treatment of articular cartilage injuries especially osteochondral tissue requires intervention of bioengineered scaffold. In this study, we investigated the potential of the tissue‐engineered cryogel scaffold fabricated using cryogelation technology. Two types of cryogels viz. chitosan‐gelatin‐chondroitin sulfate (CGC) for articular cartilage and nano‐hydroxyapatite‐gelatin (HG) for subchondral bone were fabricated. Further, novel bilayer cryogel designed using single process fabrication of two layers (CGC as top layer and HG as the lower layer) was designed to mimic osteochondral unit. CGC cryogel was tested for their biocompatibility using the enzymatically isolated chondrcoytes from goat articular cartilage while HG cryogel was tested using pre‐osteoblast cell line. Extracellular vesicles, specifically exosomes were isolated from the spent media of chondrocytes to validate their effect over cell proliferation and migration which are required for defect healing and infiltration respectively. These isolated exosomes were characterized and analyzed for confirming their size distribution profile and visualized morphologically using advanced microscopy techniques. For cartilage part, CGC cryogels were examined as delivery system for delivering exosomes at defect site, where 80% of release was observed in 72 h. Release of 18.7 µg chondroitin sulfate/mg cryogel was obtained in a period of one week from CGC cryogel (termed cryogel extract) which has chondroprotective effect. Further, effect of exosome concentration (10 and 20 µg/ml), CGC extract and combination of exosome and CGC extract (Exo‐Ex) were assessed over the chondrocytes. In addition, in vitro scratch wound assay was performed to analyse the migration capacity over the micro‐injury when treated with exosomes, cryogel extract and Exo‐Ex. The overall results thus answer key questions of therapeutic potential of chondrocyte exosomes, cryogel extract in addition to potential of CGC and HG cryogel for osteochondral repair.
Nonlinear time delay estimation based model reference adaptive impedance control for an upper-limb human-robot interaction

A nonlinear Time Delay Estimation (TDE) based model reference adaptive impedance controller was developed for Tarbiat Modares University Upper Limbs Rehabilitation Robot (TUERR). The proposed controller uses a stable reference impedance model, which produces desired dynamic relationship between applied force and position error for the robot End-effector to track the desired trajectory. TDE based model reference adaptive controller estimates unknown system dynamics and uncertainties, and the adaption law modifies the controller gains. Using a Lyapunov function was shown trajectory tracking errors in the overall system are bounded. In addition, a performance-based velocity profile proposed to modify the pace of trajectory planning considering the deviation from the desired path. Finally, the performance of the presented controller and rehabilitation process is experimentally investigated for TUERR.
Enhancer architecture-dependent multilayered transcriptional regulation orchestrates RA signaling-induced early lineage differentiation of ESCs

Abstract Signaling pathway-driven target gene transcription is critical for fate determination of embryonic stem cells (ESCs), but enhancer-dependent transcriptional regulation in these processes remains poorly understood. Here, we report enhancer architecture-dependent multilayered transcriptional regulation at the Halr1–Hoxa1 locus that orchestrates retinoic acid (RA) signaling-induced early lineage differentiation of ESCs. We show that both homeobox A1 (Hoxa1) and Hoxa adjacent long non-coding RNA 1 (Halr1) are identified as direct downstream targets of RA signaling and regulated by RARA/RXRA via RA response elements (RAREs). Chromosome conformation capture-based screens indicate that RA signaling promotes enhancer interactions essential for Hoxa1 and Halr1 expression and mesendoderm differentiation of ESCs. Furthermore, the results also show that HOXA1 promotes expression of Halr1 through binding to enhancer; conversely, loss of Halr1 enhances interaction between Hoxa1 chromatin and four distal enhancers but weakens interaction with chromatin inside the HoxA cluster, leading to RA signaling-induced Hoxa1 overactivation and enhanced endoderm differentiation. These findings reveal complex transcriptional regulation involving synergistic regulation by enhancers, transcription factors and lncRNA. This work provides new insight into intrinsic molecular mechanisms underlying ESC fate determination during RA signaling-induced early differentiation.
Neuronal Induction of Bone‐Fat Imbalance through Osteocyte Neuropeptide Y

A differentiation switch of bone marrow mesenchymal stem/stromal cells (BMSCs) from osteoblasts to adipocytes contributes to age‐ and menopause‐associated bone loss and marrow adiposity. Here it is found that osteocytes, the most abundant bone cells, promote adipogenesis and inhibit osteogenesis of BMSCs by secreting neuropeptide Y (NPY), whose expression increases with aging and osteoporosis. Deletion of NPY in osteocytes generates a high bone mass phenotype, and attenuates aging‐ and ovariectomy (OVX)‐induced bone‐fat imbalance in mice. Osteocyte NPY production is under the control of autonomic nervous system (ANS) and osteocyte NPY deletion blocks the ANS‐induced regulation of BMSC fate and bone‐fat balance. γ‐Oryzanol, a clinically used ANS regulator, significantly increases bone formation and reverses aging‐ and OVX‐induced osteocyte NPY overproduction and marrow adiposity in control mice, but not in mice lacking osteocyte NPY. The study suggests a new mode of neuronal control of bone metabolism through the ANS‐induced regulation of osteocyte NPY.
Household Water Is the Main Source of Iodine Consumption among Women in Hargeisa, Somaliland: A Cross-Sectional Study

ABSTRACT Background Iodine status surveys of women in Somaliland present widely conflicting results. Previous research indicates elevated concentrations of iodine (IQR 18–72 μg/L) in groundwater used for drinking and cooking, but the relation with iodine intake is not well characterized. Objectives We aimed to investigate the contributions of household water iodine concentration (WIC), breastfeeding, total fluid intake, hydration levels, and urine volume on urinary iodine concentration (UIC) and excretion (UIE) over a 24-h period and to define iodine status from iodine intake estimates and median UIC, normalized to a mean urine volume of 1.38 L/d (hydration adjusted). Methods The study sample comprised 118 nonpregnant, healthy women aged 15–69 y. All participants resided in Hargeisa, and 27 were breastfeeding. Data collection consisted of a 24-h urine collection, a 24-h fluid intake diary, a beverage frequency questionnaire, and a structured recall interview. We measured UIC and WIC in all urine and in 49 household water samples using the Sandell-Kolthoff reaction. Results WIC ranged between 3 and 188 μg/L, with significant median differences across the water sources and city districts (P < 0.003). Nonbreastfeeding women were borderline iodine sufficient [hydration-adjusted median urinary iodine concentration (mUIC) 109 μg/L; 95% CI: 97, 121 μg/L], whereas breastfeeding women showed a mild iodine deficiency (73 μg/L; 95% CI: 54, 90 μg/L). There were strong correlations (ρ: 0.50–0.69, P = 0.001) between WIC and UIC, with iodine from household water contributing more than one-half of the total iodine intake. Multivariate regression showed hydration and breastfeeding status to be the main predictors of UIC. Conclusions Iodine from household water is the main contributor to total iodine intake among women in Hargeisa, Somaliland. Variation in female hydration and spatial and temporal WIC may explain diverging mUIC between studies. Water sources at the extremes of low and high iodine concentrations increase the risk of subpopulations with insufficient or more than adequate iodine intake.
Early Screening for Risks of Bipolar Disorder at the Preclinical Stage.

INTRODUCTION Bipolar disorder (BD) is characterized by a high rate of prevalence in the general population varying from 0.6% to 5.84% (Yildiz 2015). BD is one of the leading causes of disability and mortality from suicide and comorbid diseases (Johnson et al. 2017). Individual symptoms of the disease in the form of cyclothymia-like mood fluctuations can be detected in adolescence and have potential for predicting risk for BD (Tijssen et al. 2010). The key issue here is untimely diagnosis of BD (Mosolov et al. 2014, Bardenshteyn et al. 2016). Early screening for risks of bipolar disorder at the preclinical stage. SUBJECTS AND METHODS The study involved 137 students aged from 18 to 20 years (mean age 18.93±0.09). The clinical-psychopathological method as well as the screening method of research were used: the Mini-International Neuropsychiatric Interview (M.I.N.I.), (Sheehan et al. 1998), the Hamilton Depression Rating Scale (HDRS 1960), the Mood Disorder Questionnaire (MDQ) (Hirschfeld 2000). The statistical data processing included descriptive statistical methods (p<0.05). RESULTS Clinical diagnostics of the responders using ICD-10 (WHO, 1992, Chapter V [F00-F99]) excluded the diagnosis of bipolar disorder. The MDQ screening method revealed a statistically significant excess of the average values for hypomania throughout the sample (M±m: 6.46±0.44; p<0.05). The total score of 64 interviewees (46.7%; 95% CI: 38.1-55.3) exceeded the threshold value (≥7). 68 responders (49.6%; 95% CI 41.0-55.3) showed one-stage manifestation of certain signs of mood rise. 72 interviewees (52.6%; 95% CI 43.9-58.3) reported absence of mood rise, associated with conflict behaviour, family problems etc. According to the HDRS scale, 45 responders (32.85%; 95% CI: 24.14-40.95) showed signs of mild depression (M±m: 6.51±0.39; p<0.05). Also, a group of responders (18.2%; 95% CI: 11.78-24.72) manifested exceeding indicators both for hypomania and depression. CONCLUSIONS According to the MDQ scale, 46.7% of the responders showed threshold values exceeding; with the one-stage manifestation of hypomania signs in 49.6% of the respondents. 32.85% of the responders showed signs of mild depression (the HAMD scale). 18.2% of the interviewees exceeded threshold values for both hypomania and depression. The discovered cyclothymia-like conditions at the preclinical stage have potential for predicting risk for their transformation to bipolar disorder which directs further outpatient clinical and dynamic observation.
mBodyMap: a curated database for microbes across human body and their associations with health and diseases

Abstract mBodyMap is a curated database for microbes across the human body and their associations with health and diseases. Its primary aim is to promote the reusability of human-associated metagenomic data and assist with the identification of disease-associated microbes by consistently annotating the microbial contents of collected samples using state-of-the-art toolsets and manually curating the meta-data of corresponding human hosts. mBodyMap organizes collected samples based on their association with human diseases and body sites to enable cross-dataset integration and comparison. To help users find microbes of interest and visualize and compare their distributions and abundances/prevalence within different body sites and various diseases, the mBodyMap database is equipped with an intuitive interface and extensive graphical representations of the collected data. So far, it contains a total of 63 148 runs, including 14 401 metagenomes and 48 747 amplicons related to health and 56 human diseases, from within 22 human body sites across 136 projects. Also available in the database are pre-computed abundances and prevalence of 6247 species (belonging to 1645 genera) stratified by body sites and diseases. mBodyMap can be accessed at: https://mbodymap.microbiome. cloud.
ID3 promotes homologous recombination via non-transcriptional and transcriptional mechanisms and its loss confers sensitivity to PARP inhibition

Abstract The inhibitor of DNA-binding 3 (ID3) is a transcriptional regulator that limits interaction of basic helix-loop-helix transcription factors with their target DNA sequences. We previously reported that ID3 loss is associated with mutational signatures linked to DNA repair defects. Here we demonstrate that ID3 exhibits a dual role to promote DNA double-strand break (DSB) repair, particularly homologous recombination (HR). ID3 interacts with the MRN complex and RECQL helicase to activate DSB repair and it facilitates RAD51 loading and downstream steps of HR. In addition, ID3 promotes the expression of HR genes in response to ionizing radiation by regulating both chromatin accessibility and activity of the transcription factor E2F1. Consistently, analyses of TCGA cancer patient data demonstrate that low ID3 expression is associated with impaired HR. The loss of ID3 leads to sensitivity of tumor cells to PARP inhibition, offering new therapeutic opportunities in ID3-deficient tumors.
How to classify all finite dimensional Hopf algebras over the Suzuki algebra

The Suzuki algebra A μλ Nn was introduced by Suzuki Satoshi in 1998, which is a class of cosemisimple Hopf algebras. It is not categorically Morita-equivalent to a group algebra in general. In this paper, the author gives a complete set of simple Yetter-Drinfeld modules over the Suzuki algebra A μλ N 2n and investigates the Nichols algebras over those simple Yetter-Drinfeld modules. The involved finite dimensional Nichols algebras of diagonal type are of Cartan type A1, A1 × A1, A2, A2 × A2, Super type A2(q; I2) and the Nichols algebra ufo(8). There are 64, 4m and m-dimensional Nichols algebras of non-diagonal type over A μλ N 2n . The 64-dimensional Nichols algebras are of dihedral rack type D4. The 4m and m -dimensional Nichols algebras B(Vabe) discovered first by Andruskiewitsch and Giraldi can be realized in the category of Yetter-Drinfeld modules over A μλ Nn . By using a result of Masuoka, we prove that dimB(Vabe) = ∞ under the condition b = (ae)−1, b ∈ Gm for m ≥ 5.
Design of a seed implantation robot with counterbalance and soft tissue stabilization mechanism for prostate cancer brachytherapy

This article focuses on the topic of the structural design of surgical radioactive surgery robot for prostate cancer. To improve the weight-to-payload ratio of surgery robot end-effector, the energy consumption and stability of robot joint drive and reducing the displacement and deformation of needle insertion in soft tissue. This article discusses the new static torque balancing method and multi-needle insertion soft tissue stabilization mechanisms that may be used in previously articulated seed implantation robots. Compared with the existing balancing system schemes, we adopt the idea of mutual conversion of gravitational potential energy and elastic potential energy and establish a static balancing model. With preloaded displacement parameter of the spring α, the variable gravity torque balance of robot arm can be achieved. Torque and equivalent gravity balancing distribution with the spring balance system and the quantitative evaluation experiment were performed, and experiment results provide evidence that these spring balance devices can basically compensate the gravity torque of the robot arm. In addition, we used nonlinear spring–damper model to establish multi-needles insertion soft tissue force model. Then, a variable multi-needle insertion soft tissue stabilization device is designed with six working modes. The innovative design of this device is the use of the first four needles that are introduced simultaneously on either side of the midline. Initially completed displacement simulation of different numbers of needle insertion prostate tissue, experiment results indicate that multi-needle puncture mechanism could reduce prostate displacement in the y- or z-direction. By this method, the prostate may be fixed, thus this mechanism maybe reduces rotation of the prostate and enabling subsequent needles to be inserted accurately.
Development of an ergonomic wearable robotic device for assisting manual workers

Sometimes the automation equipment cannot solve all the problems for industrial enterprises, and human workers cannot be replaced by machines in production activities. The possibility that the workers develop work-related musculoskeletal disorders, while performing high intensity and repetitive installation and commissioning work over a long period of time, is very high. A mechanical design of a passive upper extremities exoskeleton suit to reduce the muscles effort of upper limbs is proposed in this article. Thereby, a decrease in the work-related musculoskeletal disorders risk is expected. To evaluate the ergonomic contribution of the passive upper extremities exoskeleton suit, both static and dynamic tool lift experiments were designed, in which 10 volunteers were asked to participate in the experiments. The surface electromyography is captured from these volunteers to measure the magnitude of muscle output forces that are applied with and then without passive upper extremities exoskeleton suit assistance during the process of manual handling, and the tests are collected for comparison. Results show that there is a significant decrease in the output force and fatigue in deltoid, biceps brachii, and brachioradiali, especially in biceps brachial which is up to 67.8%. The implementation of passive upper extremities exoskeleton suit is not only a benefit to reduce workers’ upper extremities fatigue but also ultimately increase the work efficiency by minimizing work-related musculoskeletal disorders and safety accidents.
Constellation-shaped three-dimension 14QAM mapping scheme based on regular tetrahedron cells

Abstract. An innovative constellation-shaped three-dimensional 14-quadrature amplitude modulation QAM (3D-14QAM) mapping scheme based on regular tetrahedron is proposed. The non-uniform 14QAM signals are designed by Huffman code and mapped onto the designed 3D constellation based on regular tetrahedron. The simulation demonstration is carried out successfully by VPI software in a 4 × 100 Gb / s wavelength division multiplexing (WDM) polarization division multiplexed coherent optical communication system with a transmission distance of 300 km. For WDM system, the simulation results show that the proposed scheme is capable of achieving about 1.8 dB OSNR gain over the traditional 3D-16QAM scheme at the hard-decision forward error correction (HD-FEC) limit of 3.8 × 10 − 3. In addition, the proposed 3D-14QAM can obtain 1.1 dB OSNR gain compared with the traditional 3D-16QAM for single-channel scenario at the HD-FEC. The outstanding performance indicates that the proposed scheme can provide a performance improvement for both single carrier system and WDM system. And benefited from the ability of suppress nonlinearity, the proposed scheme can obtain a larger gain in the WDM system than a single carrier system.
Determination of reference ranges for automated erythrocyte and reticulocyte parameters in healthy adults

Abstract Objectives Recent advances in hematology analyzers have enabled to improve the reliability in the results and also provided additional hematological parameters. In the present study, we aimed to determine the reference ranges for automated erythrocyte and reticulocyte parameters in healthy individuals on Sysmex XN 1000 hematology analyzer. Methods One hundred and thirty-three subjects with normal physical examination and complete blood count results within the reference ranges were included in the study. Venous blood samples collected in tubes containing K2-EDTA were analyzed on Sysmex XN-1000. The references intervals for IRF, RBC He, Ret He, LFR, MFR, HFR, Delta He, Micro R, Macro R, Hypo He and Hyper He were determined according to CLSI EP28-A3c. Results The reference ranges of the parameters were estimated with 90% confidence intervals. The reference ranges were 3.4–17% for IRF, 26.9–32.8 pg for Ret-He, 25.2–30.5 pg for RBC-He and 0.5–3.7 pg for delta-He. Gender specific reference ranges were calculated for of Ret-He (male (M): 26.8–32.9 pg, female (F): 23.9–33.6 pg), RBC-He (M: 26.3–30.8 pg, F: 25.3–30.5 pg) and delta-He (M: 0.5–3.7 pg, F: 0.3–3.7 pg). Conclusions The new reticulocyte and erythrocyte parameters may be conveniently used in clinical diagnosis and follow-up of patients, as they offer reliable, automated and cheap results. Each laboratory is recommended to determine its own reference intervals considering the differences like the instrument used and population studied.
The parallel lives of autonomous systems: ASN allocations vs. BGP

Autonomous Systems (ASes) exist in two dimensions on the Internet: the administrative and the operational one. Regional Internet Registries (RIRs) rule the former, while BGP the latter. In this work, we reconstruct the lives of the ASes on both dimensions, performing a joint analysis that covers 17 years of data. For the administrative dimension, we leverage delegation files published by RIRs to report the daily status of Internet resources they allocate. For the operational dimension, we characterize the temporal activity of ASNs in the Internet control plane using BGP data collected by the RouteViews and RIPE RIS projects. We present a methodology to extract insights about AS life cycles, including dealing with pitfalls affecting authoritative public datasets. We then perform a joint analysis to establish the relationship (or lack of) between these two dimensions for all allocated ASNs and all ASNs visible in BGP. We characterize the usual behaviors, specific differences between RIRs and historical resources, as well as measure the discrepancies between the two "parallel" lives. We find discrepancies and misalignment that reveal useful insights, and we highlight through examples the potential of this new lens to help pinpoint malicious BGP activity and various types of misconfigurations. This study illuminates a largely unexplored aspect of the Internet global routing system and provides methods and data to support broader studies that relate to security, policy, and network management.