Miracle-dz/UniFAR-FTUs huggingface dataset:
Nuclear location signal peptide–modified poly (ethyleneimine)/DNA complexes: An efficient gene delivery vector in vitro and in vivo

The low transfection efficiency of nonviral gene delivery systems limits their applications. In this study, we demonstrated a simple method to modify poly(ethyleneimine)/DNA complexes with a nuclear location signal peptide via bis(succinimidyl) penta(ethylene glycol) coupling. The amount of grafted nuclear location signal peptide was controlled within a range of 0–9 µg for poly(ethyleneimine)/DNA complexes containing 10 µg DNA and 100 µg poly(ethyleneimine) by adjusting the grafting agent and peptide feeds. The particle size and surface zeta-potential of the complexes were largely retained after nuclear location signal immobilization. Based on the results of the flow cytometry measurements, the nuclear location signal–modified poly(ethyleneimine)/DNA complexes were internalized into at bone marrow stem cells at a significantly faster rate and a higher amount than the unmodified complexes. In vitro transfection experiments, using plasmid DNA encoding bone morphogenetic protein 2, indicated that the nuclear location signal peptide–modified poly(ethyleneimine)/DNA complexes have significantly higher gene transfection ability toward bone marrow stem cells than unmodified complexes. The porous collagen scaffolds loaded with nuclear location signal–modified poly(ethyleneimine)/plasmid DNA encoding bone morphogenetic protein 2 complexes successfully transfected tissue cells and induced the human bone morphogenetic protein 2 expression in a rat. The modification of the poly(ethyleneimine)/DNA complexes with nuclear location signal peptide was effective in enhancing gene transfection of complexes in vitro and in vivo, thus indicating potential applications for bioactive scaffolds with enhanced tissue regeneration performance.
Synthesis, Characterizations and Biological Screening of Tetrahydro-Quinazoline Analogues

Quinazoline and their fused-ring systems are well known for their potential biological activity. In the present study a new Tetrahydro-quinazoline analogues (MB I-V) were synthesized. The newly synthesized compounds were characterized by IR, NMR and C, H, N, S analyses. All newly synthesized compounds were screened for their antibacterial (Pseudomonas aurigenosa Bacillus subtilis and Escherichia coli) studies. The results revealed that all synthesized compounds have a significant biological activity against the tested microorganisms.
Dipole-Field Interactions Determine the CO2 Reduction Activity of 2D Fe–N–C Single-Atom Catalysts

Electrochemical CO2 Reduction (CO2R) can potentially allow for the sustainable production of valuable fuels and chemicals. Recently, single atom catalysts on a 2D support have been shown to be a promising catalyst candidate. Using state-of-the-art methods, we develop a model for Fe doped graphene which rationalises several critical experimental observations: the contentious origin of the pH dependence of reactivity and the dependence of current-potential relationships on active site. We show that single atom catalysts have the unique ability to stabilise different dipoles associated with critical reaction intermediates, which translates to significant shifts in activity. This provides a new rational design principle and paves the way for rigorous computation-guided catalyst design of new single atom catalysts for CO2R.
TP53 dysfunction in chronic lymphocytic leukemia: clinical relevance in the era of B-cell receptors and BCL-2 inhibitors

ABSTRACT Introduction Patients with TP53 dysfunction, assessed by del(17p) or TP53 mutations, respond poorly to chemo-immunotherapy and fare better with the new therapies (BCR and BCL-2 inhibitors); however, it is unclear whether their response is similar to that of patients without anomalies or whether there is currently an adequate determination of TP53 dysfunction. Area covered A literature search was undertaken on clinical trials and real-world experience data on patients with TP53 dysfunction treated with different protocols. Moreover, data on the TP53 biological function and on the tests currently employed for its assessment were reviewed. Expert opinion Although TP53 dysfunction has less negative influence on the new biological therapies, patients with these alterations, particularly those with biallelic inactivation of TP53, have a worst outcome with these therapies than those without alterations. At present, a determination of TP53, particularly with next generation sequencing (NGS) methodologies, may be sufficient for the identifications of the patients unsuitable for chemo-immunotherapy, although integration with del(17p) would be advisable. For the future, more extensive determinations of the TP53 status, including functional assays, may become part of the current armamentarium for a better patient stratification and treatment with newer protocols.
Group Learning Through the Lens of Learner Autonomy

Learner autonomy describes the individual who exercises personal agency in his or her learning. Similar to an individual, groupsiathat is, collections of people with a defined purpose often represented by organizational subunitsiaalso engage in intentional learning to support and improve existing processes or identify and pursue new opportunities. The purpose of this theoretical discussion is to characterize aspects of intentional group learning using the theoretical framework associated with learner autonomy. Applied to the group level, notions of agentic learning, modes through which agentic learning is exercised, and regulation will be discussed through this lens of autonomy. This discussion may prove useful in describing how organizational groups learn intentionally and in developing processes that improve such learning.
Hα Luminosity of ATLAS18qtd Does Not Plateau in the Nebular Phase

We present new spectroscopic and photometric observations of ATLAS18qtd/SN 2018cqj, a fast-declining SN Ia with variable Hα emission in previously published nebular phase spectra. ATLAS18qtd is undetected in both spectroscopic and photometric observations which occurred at ∼540 days after maximum light and ∼230 days after the last Hα detection. With these new non-detections, we place an upper limit on the Hα luminosity of ≲ 1.1 × 10 36 erg s − 1 indicating the Hα flux decreased by a factor of ≳ 4 since the previous detection. This upper limit excludes Hα emission that plateaus or increases since the previous detection but cannot confirm that the Hα emission decay rate is equivalent to the supernova decay rate.
Effects of ascorbic acid and erythorbic acid on melanosis and quality in different shrimp species

With this study, ascorbic acid and erythorbic acid were used for the first time to prevent melanosis in shrimp. Three shrimp species ( Aristaeomorpha foliacea , Plesionika edwardsi and Melicertus hathor ) were used. It was determined that melanosis scores were higher in the control groups. Combination of ascorbic acid and erythorbic acid with metabisulphite was found to be more effective in inhibiting of melanosis than the application alone. No significant differences were found between the groups in terms of TVB-N values. The TMA-N value of the control group was significantly higher than those of application groups. Ascorbic acid, Erythorbic acid and their combinations with sodium metabisulphite were found effective on inhibition of melanosis and quality changes in three shrimp species. Keywords: reducing agents; ascorbic acid; erythorbic acid; melanosis; quality; shrimp
Brain responses to change in phonological structures of varying complexity in children and adults.

Language-related change-detection processes are often investigated using syllables that are very simple in terms of phonological structure. However, phonological complexity is known to be challenging for young typically developing children and pathological populations. We investigated brain correlates of phonological processing and their age-related changes with a passive change-detection protocol including stimuli of varying phonological complexity, which allowed comparing responses to simple and complex phonological deviancies. Mismatch Negativity (MMN) and Late Discriminative Negativity (LDN) responses were recorded in both school-age children (n = 22) and adults (n = 24). MMN was similar for simple and complex phonological deviancy in both groups, whereas LDN appeared to be modulated by phonological complexity, albeit with different patterns according to age. In response to complex phonological change, children displayed a larger LDN response with a typical fronto-central scalp distribution, while adults showed an additional right-posterior activity but no larger amplitude than for simple change. Thus, LDN appears to be a good electrophysiological index of phonological complexity processing. This study validated the use of the LDN through this protocol for the investigation of phonological complexity processing throughout the development.
Instructional Feedback: An Effective, Efficient, Low-Intensity Strategy to Support Student Success

Instructional feedback is a practical strategy for monitoring student learning in response to instruction and providing prompts to students to confirm, refine, or clarify their misunderstandings. In this article, we describe a step-by-step process for using instructional feedback, with teaching tips to assist successful implementation with all students, including those with emotional and behavioral disorders. Throughout the article, we offer lessons from practitioners who have successfully used instructional feedback in their classrooms.
Mariposas de la familia Pieridae (Lepidoptera: Papilionoidea) de un bosque seco tropical en la cuenca del río Playonero, Santander, Colombia

Colombia is a privileged country given the geographical position, the diversity of ecosystems and the great complexity in plant communities, conditions that allow it to occupy the third place in diversity of butterflies on the World. We establish the composition of species of family Pieridae in four sites of Playonero river basin, located in the northeast of the Colombian Andes in the department of Santander. We made captures of Pieridae butterflies in the project Characterization of the wild Entomofauna of Playonero river basin. We collected 84 individuals belonging to four genera and 12 species. The analysis of sampling quality estimated a potential richness of 14.51 species, a proportion of observed species of 82.71% and a sampling effort of 90.71%. The analysis of similarity shows a grouping of La Tigra, Planadas and La Naranjera. We report the presence of Eurema gratiosa (Doubleday, 1847) and Perrhybris lypera sulphuralis (Butler, 1896), thus expanding the list of species registered in Colombian dry forests. Villalobos-Moreno et al.: Pieridae de un bosque seco tropical en Santander, Colombia. 304
Accounting for fish shoals in single- and multi-species survey data using mixture distribution models

A scientific bottom trawl survey targeting Pacific rockfishes (Sebastes spp.) occasionally yields extraordinary catch events (ECEs) in which catch-per-unit-area is much greater than usual. We hypothesize that ECEs result from trawl catches of fish shoals. We developed mixture distribution models for positive catch rates to identify spatial covariates associated with ECEs or normal trawl catches and used simulation modeling to contrast the performance of mixture distribution and conventional log-linear models for estimating an annual index of positive catch rates. Finally, mixed-effects modeling was applied to multispecies data to evaluate the hypothesis that ECEs are related to shoaling behaviors. Results show that mixture distribution models are often selected over conventional models for shoaling species and that untrawlable habitat has a positive effect on rockfish densities. Simulation shows that mixture distribution models can perform as well as or better than conventional models at predicting positi.
Conservation Genetics of the Threatened Bayou Darter (Percidae: Etheostoma rubrum) in the Bayou Pierre System of Southwestern Mississippi

Abstract The Bayou Darter, Etheostoma rubrum, is endemic to the Bayou Pierre system of southwestern Mississippi where it occupies swift, shallow riffles with coarse, firm substrata. The Bayou Pierre system has experienced extensive erosion in response to rapid headcutting leading to loss of riffle habitat and degradation of riverine conditions. Due to its high degree of habitat specificity along with ongoing habitat fragmentation and potentially reduced gene flow between isolated populations, the Bayou Darter is vulnerable to severe population declines and possible extinction. Our objectives were to quantify levels of genetic diversity in the mitochondrial control region and infer population structure across the range of the species. Sequencing of 106 sampled individuals revealed only three mtDNA haplotypes with one variable site. Haplotype diversity and nucleotide diversity were low (h ≤ 0.11 and π < 0.001), and there was no structure revealed among the four sampled populations. The low genetic diversity in E. rubrum may best be explained by a small range size and recent genetic bottleneck.
A Design of Wide-Range and Low Phase Noise Linear Transconductance VCO with 193.76 dBc/Hz FoMT for mm-Wave 5G Transceivers

This paper presents a wide-range and low phase noise mm-Wave Voltage Controlled Oscillator (VCO) based on the transconductance linearization technique. The proposed technique eliminates the deep triode region of the active part of the VCO, and lowers the noise introduced by the gm-cell. The switch sizes inside the switched capacitor bank of the VCO are optimized to minimize the resistance of the switches while keeping the wide tuning range. A new layout technique shortens the routing of the VCO outputs, and lowers the parasitic inductance and resistance of the VCO routing. The presented method prevents the reduction of the quality factor of the tank due to the long routing. The proposed VCO achieves a discrete frequency tuning range, of 14 GHz to 18 GHz, through a linear coarse and middle switched capacitor array, and offers superior phase noise performance compared to recent state-of-the-art VCO architectures. The design is implemented in a 45 nm CMOS process and occupies a layout area (including output buffers) of 0.14 mm2. The power consumption of the VCO core is 24 mW from the power supply of 0.8 V. The post-layout simulation result shows the VCO achieves the phase noise performances of −87.2 dBc/Hz and −113 dBc/Hz, at 100 kHz and 1 MHz offset frequencies from the carrier frequency of 14 GHz, respectively. In an 18 GHz carrier frequency, the results are −87.4 dBc/Hz and −110 dBc/Hz, accordingly.
The Efect of Green Harvest on the Quality of Organic Grapes Cultivated in Murfatlar Viticultural Centre

Abstract In viticulture, the control of the crop load is largely used as an important instrument for the regulation of yield and grape quality, which in turn leads to quality wines. One of the practices for the regulation of crop load, often used for table grapes, is the green harvest, that is, the removal of some unripe bunches in order to drive more of the vines' strength towards the remaining clusters. At present this practice is also applied for wine grapes, regulating in this way the yield and the quality of grapes and wines, due to a better accumulation of sugar in the berries of the remaining bunches and a better accumulation of aromatic and colour compounds into the grape skins. In this paper we present the results regarding the usability and agro-biological behaviour to green harvesting of two vine cultivars (Feteasca neagra and Cabernet Sauvignon) grown in organic plantations in Murfatlar vine region, as compared to controls without cluster thinning. The main parameters followed were the indicators of grape quality (sugar concentration, total acidity, pH, anthocyanins and total polyphenolic index) and the technological parameters (the average weight of the cluster, the weight and number of the berries, the rachis weight and the yield per vine). The statistical analysis of the results showed that the green harvest had a beneficial effect on the grape composition and wine quality for both varieties.
Antibacterial Activities of Some Plant Extracts Used in Folk Medicine

The antibacterial activities of ethyl acetate, methanol, chloroform, and acetone extracts of four plant species were studied. The dried extracts of the whole plant of Artemisia absinthium (Compositae/Asteraceae) and Urtica dioica (Urticaceae), flowering plants of Fumaria officinalis (Papaveraceae/Fumariaceae) and the leaves of Rosmarinus officinalis (Labiatae/Lamiaceae) were tested in vitro against 12 bacterial species and strains by the agar diffusion method. Bacillus brevis FMC 3, Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus subtilis var. niger ATCC 10, Micrococcus luteus LA 2971, Mycobacterium smegmatus RUT, Escherichia coli DM, Listeria monocytogenes SCOTT A, Staphylococcus aureus ATCC 25923, Streptococcus thermophilus, Pseudomonas fluorescens, and Yersinia enterocolitica O:3 P 41797 were used in this investigation. The results indicated that neither the whole plant extracts of Urtica dioica nor Fumaria officinalis showed antibacterial activity against the test micro-organisms. All the extracts of the leaves of Rosmarinus officinalis showed various inhibitory effects (7-16 mm/20 µl inhibition zone), except the acetone extract against Yersinia enterocolitica. The whole plant ethyl acetate and chloroform extracts of Artemisia absinthium inhibited some of the test micro-organisms (8-16 mm/20 µml inhibition zone).
Robust adaptive fuzzy sliding mode controller for nonlinear uncertain hysteretic systems

This paper deals with the problem of adaptive fuzzy control for a class of nonlinear uncertain systems with hysteresis input. Fuzzy logic systems are employed to approximate the unknown nonlinear behaviors, and the sliding mode technique is used to synthesize an adaptive fuzzy controller. A proportional integral control term is adopted to reduce the chattering phenomenon engendered by both sliding mode control technique and hysteretic characteristic of the system. The proposed control scheme ensures the boundedness of all closed-loop signals, and forces the tracking error to converge to zero. The main contribution of this work is the development of a control strategy for a class of nonlinear hysteretic systems subject to external disturbances and uncertainties. Two case studies are given to illustrate and to prove the effectiveness of the presented approach.
Preparation and consumer acceptability tests of fermented camel milk in Kenya

There are estimated to be 600000 camels (Camelus dromedarius) in Kenya (Wandera, 1985). Almost 80% of these are kept by pastoral tribes living in arid areas in eastern and north-eastern parts of the country. In these regions, camels are important dairy animals. A camel in north-east Kenya can be expected to yield about 4 kg milk daily as compared with 0·5–1·5 kg for a cow in the same area. Most of the camel milk is consumed in the form of fermented milk. The milk is allowed to ferment naturally at ambient temperature and without prior heat treatment until it turns sour. The resulting fermented camel milk is known as Susa. Due to the spontaneous nature of the fermentation, this traditional method results in a product with varying taste and flavour and is often of poor hygienic quality, in addition, because of the limited scale of production, the product can be sold only in the immediate vicinity of the herd. For production of fermented milk under controlled conditions, thermophilic or mesophilic lactic acid cultures are normally used. In warm countries, mesophilic lactic cultured milk offers some advantages, as it can be incubated at ambient temperature (20–30 °C) and the fermentation stops at 1–1·2% lactic acid, eliminating the need for cooling to stop further souring as occurs in the case of yoghurt (Kurwijila, 1980).
Effect of Ethinylestradiol on hsp70 Expression in Transgenic Drosophila melanogaster ( hsp70-lacZ) Bg 9

Summary In the present study the effect of 0.25, 0.50, 1.0 and 2.0 �l/ml of ethinylestradiol was studied on 3 rd instar larvae of transgenic Drosophila melanogaster ( hsp70-lacZ) Bg 9 for 6, 24 and 48 hrs of duration. The treatment of 0.25 �l/ml of ethinylestradiol did not induce significantly the activity of hsp70 as compared to control. The treatments of 0.5, 1.0 and 2.0 �l/ml of ethinylestradiol induced significant increase in the activity of hsp70 for the different durations of exposure. The results of the present study suggest that the doses of 0.5, 1.0 and 2.0 �l/ml of ethinylestradiol are cytotoxic in the 3 rd instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9 .
Actual or Perceived Abundance? Interpreting Annual Survey Data in the Face of Changing Phenologies

Abstract. Many annual monitoring programs assume that availability—the probability that an animal will be visible, make an audible sound, or leave other evidence of its presence—is not changing systematically from year to year. Until recently, this assumption of unchanging availability seemed reasonable, but recent studies report changes in breeding phenology that are presumed to be linked to climate change. Because the rate of bird song is often correlated with stage of breeding, earlier breeding could shift timing of peak bird availability, changing the number of birds counted during annual surveys on set dates. Such changes could be erroneously interpreted as population trends. To better understand how changes in phenology might affect the probability of detecting birds, we modeled availabilities of 31 species in southern Alabama through the breeding season, documenting strong seasonal variation in availability. Then, using our availability estimates, we investigated whether changes in detection probabilities could underlie observed changes in the abundances of some species. We calculated the expected change in the number of times a species would be recorded during surveys conducted within fixed dates by assuming a 1-week shift in breeding activity. We found that summer residents were more likely to show changes in availability but that such changes in availability did not account for trends in Breeding Bird Survey (BBS) data for Alabama. Our results suggest that while summer residents' availability may be declining during BBS dates, population declines observed in BBS data for Alabama cannot be dismissed as due to shifting phenology.
How to inspire customers via social media

Social media has emerged as a new marketing channel and an important source for customer inspiration. Understanding the sources and consequences of customer inspiration via social media is important for both researchers and marketers. However, little has been done to empirically examine customer inspiration in the social media context. Specifically, the process through which customers become inspired and motivated by social media to purchase the advertised products remains elusive. Therefore, this study extends the conceptualisation of customer inspiration into the social media context, and explores the antecedents of customer inspiration from three perspectives: informational, social and personal. This study also examines the impact of customer inspiration on customers' behavioral intention. ,Survey methodology was used and data from 679 respondents were collected in China. To examine the hypothesized relationships, partial least squares (PLS) was used to analyse the data and Smart PLS 3.0 was used for data analysis. ,The study findings demonstrated that the vividness of ads, social influence and promotion focus had positive impacts on the “inspired-by state”, and subsequently the “inspired-to state”, which in turn positively influenced customers' purchasing intentions. However, media richness has a negative impact on the “inspired-by state”. ,This study provides the first evidence of customer inspiration via social media and examines its antecedents and consequences. Practically, this study offers useful guidelines and suggestions for firms on how to inspire customers using social media.