Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Characterization of Antibiotic Resistant Bacteria from Soil within Rivers State University, Nigeria

Antibiotic resistance has become a subject of global concern to health systems all over the world and the shedding of antibiotic resistant bacteria from humans and animals into the soil is a threat to public health. This research was carried out to molecularly characterize and assess antibiotic resistant bacteria from soil in Rivers State University. Bacteria from soil in four locations; waste dump site, fish farm, poultry farm and home soil were enumerated and identified using standard microbiological techniques such as serial dilution, plating and incubation, isolation, biochemical testing and molecular identification. Sensitivity testing on the bacterial isolates was done using Kirby-Bauer Disk diffusion method. These resistant isolates were also subjected to molecular testing to ascertain the presence of resistant genes. The results revealed mean total Heterotrophic Bacterial Counts (THBC) for 32 samples ranged from 1.05±0.72 x 107 to 3.63±2.58x 107 CFU/g in fish farm and waste dump samples respectively. Total Coliform Counts (TCC) ranged from 3.65±2.91 x105 to 6.92±4.41 x105 CFU/g in Fish farm and Waste dump samples while Total Staphylococcal count ranged from 3.80±1.71 x104 to 11.46±9.21 x104 CFU/g in Fish farm and Waste dump samples respectively. Total Pseudomonad Count (TPC) ranged from 0.09±0.09 x 103 to 2.47±1.67x 103 CFU/g in Home soil and Fish farm samples. There was a difference (p≤0.05) in total heterotrophic bacterial, Staphylococcal and Pseudomonad counts, but no difference (p≥0.05) in total coliform. Seventy-nine (79) bacterial isolates were identified in this study belonging to the following genera; Bacillus spp, Staphylococcus spp. , Micrococcus spp, Pseudomonas spp, Serratia spp, Proteus spp, Klebsiella spp, Salmonella spp and Escherichia spp with Staphylococcus (58.83%) having the highest occurrence and Pseudomonas (11.11%) had the least occurrence from the samples. Antibiotic sensitivity revealed that most isolates were resistant to many antibiotics tested with the highest resistance observed for Cefuroxime, Ceftazidime and Cefixime (100%) for the gram negative organisms and also Cefuroxime, Ceftazidime and Cloxacillin for gram positive bacteria. However, all bacteria showed sensitivity to gentamicin. Multiple Antibiotic resistance (MAR) index for all bacteria were above 0.2. Resistant bacterial isolates were identified molecularly as S. aureus, S. sciuri, M. luteus, B. cereus, B. subtilis, P. aeruginosa, S. mercescens, P. vulgaris, E. coli and Salmonella typhimurium. Pseudomonas aeruginosa, Serratia mercescens, Proteus vulgaris and E. coli had CTX-M gene present in their genome while Staphylococcus aureus, Staphylococcus sciuri, Micrococcus luteus, Bacillus cereus and Bacillus subtilis had the mecA gene present in their genome. This study has highlighted the rise in antibiotic resistance in bacteria from soil, hence, there is need to checkmate indiscriminate use of antibiotics in agriculture.
Under Oshun’s Gaze: Africana religions as a model for repatriation

ABSTRACT Questions about museums’ responsibilities to return looted African religious artifacts in their collections continue to create a number of challenges for US museums. This article assesses these challenges from the perspective of the Brooklyn Museum and the Fowler Museum at UCLA. While pioneering two different strategies, they both reimagine the restitution process as one where source communities and museum professionals work collaboratively together to cultivate relationships around the religious cosmologies of Africana religions and provide important case studies into the processes by which museums might move toward “propatriation”, a process that involves commissioning new artistic creations and repairing relationships. Ultimately, the Brooklyn Museum and the Fowler Museum demonstrate how the religious contexts of African ritual objects can serve as important catalysts for the transformation of museums into ritualized healing spaces where the remaking of relationships is made possible as is the recognition of museums’ participation in colonial violence and theft.
Insights on the evolution of Coronavirinae in general, and SARS-CoV-2 in particular, through innovative biocomputational resources

The structural proteins of coronaviruses portray critical information to address issues of classification, assembly constraints, and evolutionary pathways involving host shifts. We compiled 173 complete protein sequences from isolates belonging to the four genera of the subfamily Coronavirinae. We calculate a single matrix of viral distance as a linear combination of protein distances. The minimum spanning tree (MST) connecting the individuals captures the structure of their similarities. The MST re-capitulates the known phylogeny of Coronovirinae. Hosts were mapped onto the MST and we found a non-trivial concordance between host phylogeny and viral proteomic distance. We also study the chimerism in our dataset through computational simulations. We found evidence that structural units coming from loosely related hosts hardly give rise to feasible chimeras in nature. This work offers a fresh way to analyze features of SARS-CoV-2 and related viruses.
Cytoplasmic p53 aggregates accumulated in p53-mutated cancer correlate with poor prognosis

Abstract Recent studies suggested that aggregates of mutant p53 proteins may propagate and impair normal p53 functioning in recipient cells. Our previous study showed that cancer cell-derived p53 aggregates that cells internalized interfered with p53-dependent apoptosis in recipient cells. However, involvement of p53 aggregate propagation in cancer pathology has not been fully elucidated. Here, we screened patients with high-grade serous ovarian carcinoma, which is characterized by an extremely high frequency of TP53 gene mutations, to show that patients with cytoplasmic p53 deposits have a poor prognosis compared with patients with complete p53 absence or strong nuclear p53 positivity. Cytoplasmic p53 in the patients with poor prognosis consisted of protein aggregates, which suggests that p53 aggregates are oncogenic drivers. Indeed, an inhibitor of p53 aggregation restored cellular apoptosis, a proper p53 function, in p53 aggregate-bearing patient-derived tumor organoids. In cell-based assays, endogenous and exogenous mutant p53 aggregates hindered chemotherapeutic activity of cisplatin, which depends on normal p53 functions. This inhibition was reduced by blocking p53 aggregation or internalization of p53 aggregates. Our study, thus indicates the involvement of p53 aggregate transmission in poor prognosis and in chemotherapy resistance in cancers.
Strategic Location Based On the Availability of Public Facilities Using Multiple-Buffer

Determining strategic location requires some considerations. One of them is to consider the availability of public facilities. This research will find out the strategic location in the East Jakarta area. Land Suitability Index suggests the strategic location can be regarded as from the availability of public facilities. The public facilities are health facilities, schools, public transportation, grocery, and restaurant. This research uses multiple ring buffers to create a map that shows the distance values of each public facility. The distance values will be shown in the form of multiple buffers, with the position of the public facility will be the center point of the buffer. The time needed to travel to the location will be the parameters for the multiple-ring buffers to create the buffers. From the datasets that have been put into the map and using the multiple ring buffers to show the distance values of the public facilities, this research finds out that several areas in East Jakarta can be considered the strategic location and not the strategic location. If there is a lot of buffer in the area, then the area can be regarded as a strategic location. Otherwise, if there is only a little or even no buffer in the area, then the area can be considered as a not strategic location. The research finds that area around Salemba Raya and Kayu Manis street, around Pahlawan Revolusi street, and around Bekasi Timur and Jendral Basuki Rahmat street are the strategic location. Meanwhile, the area around Naga Raya, Rawa Sumur, and between Otista street and Jagorawi highway are considered not strategic locations.
Fine Control of In Vivo Magnetic Hyperthermia Using Iron Oxide Nanoparticles with Different Coatings and Degree of Aggregation

The clinical implementation of magnetic hyperthermia has experienced little progress since the first clinical trial was completed in 2005. Some of the hurdles to overcome are the reliable production of magnetic nanoparticles with controlled properties and the control of the temperature at the target tissue in vivo. Here, forty samples of iron oxide superparamagnetic nanoparticles were prepared by similar methods and thoroughly characterized in terms of size, aggregation degree, and heating response. Selected samples were intratumorally administered in animals with subcutaneous xenografts of human pancreatic cancer. In vivo experiments showed that it is possible to control the rise in temperature by modulating the field intensity during in vivo magnetic hyperthermia protocols. The procedure does not require sophisticated materials and it can be easily implemented by researchers or practitioners working in magnetic hyperthermia therapies.
Classificação Inteligente do Teste de Corte do Cacau com Redes Neurais Convolucionais Profundas

Este trabalho considera a classificação automática de amêndoas de cacau no Teste de Corte como uma tarefa de Visão Computacional abordada com Redes Neurais Convolucionais. Com uma base de dados com exemplos de 14 classes, foram realizados experimentos com validação cruzada holdout os quais mostraram as redes Inception, VGG-16 e EfficientNetB0 como tendo melhor desempenho para a tarefa (acurácia média igual a 68,28 % ± 3,21, 65,54 % ± 3,05 e 55,04 % ± 2,03, respectivamente). Tais modelos foram combinados em um comitê com votação suave cuja acurácia média foi igual a 89,79 % ± 0,92, o que superou os modelos individuais avaliados. Este é um resultado promissor que pode apoiar objetivos estratégicos na melhoria da qualidade da produção do cacau no Brasil.
Constructing a Software Tool to Optimize Performance by Coupling Detection

Coupling relations reflect the interdependencies of software modules and can be used to assess a program's quality— Lower the coupling value will be, the higher the quality of the software will be. Coupling measures are crucial for determining the quality of object-oriented software, from design up to maintenance. Inside software engineering, quality attributes are realized non-functional requirements utilized to assess whether the software is of good quality or not, One of the quality attributes is Performance, which means the system can respond to various actions in a given time or how fast does it respond or execute. To calculate the total amount of time a program will require to run until completion use Time Complexity. In this paper a Computer Aided Software Engineering Tool has been constructed which is called CDPI (Coupling Detection to Performance Improvement). It parse a software source code to extract information about its structure, components, and relationships that connect its parts. That leads to improving the software readability, understandability and for detects coupling among classes of Java software.The CDPI tool detects coupling in software source code and Show it in table to the software engineer, To start the process of decoupling by the software engineer for the candidate coupling. The CDPI tool was tested by inputting software written in OOP by Java language. The CDPI Tool were evaluated by calculating the Time Complexity before and after decoupling. Results, source code after the decoupling is executed faster than before decoupling to produce the required output, which is an indication of Optimize Runtime Performance of the program.
Teriparatide as an Effective Nonsurgical Treatment for a Patient with Basicervical Peritrochanteric Fracture Nonunion—A Case Report

The nonunion rate of surgically treated basicervical peritrochanteric fractures has been reported to be as high as 9%. Due to the high 1-year mortality rate following revision surgery, finding an effective nonsurgical treatment option is of interest. Over the last decade, numerous reports have been published that have suggested teriparatide as an effective treatment for certain types of fracture nonunion. However, the literature focused on teriparatide treatment for proximal femoral fracture nonunion is scanty. A 70-year-old man suffering from a left hip basicervical peritrochanteric fracture received cephalomedullary nail fixation. Nine months after the surgery, the patient still complained of left hip pain referring to the medial thigh with an antalgic limping gait. No sign of healing was noted for more than a consecutive 3 months of follow-up. Fracture nonunion was diagnosed and further confirmed by the computed tomography (CT). The patient preferred nonsurgical treatment after thorough discussion. He then received 4 months of subcutaneous teriparatide injections, 20 mcg daily. After less than 4 months of teriparatide treatment, a follow-up CT confirmed fracture union and the patient’s pain subsided. The patient also tolerated independent ambulation afterward. Teriparatide has been reported to be an effective treatment for certain types of fracture nonunion. Our case goes a step further to expand its possible application for basicervical peritrochanteric fracture nonunion. However, further larger scale studies are needed to confirm its efficacy.
Mikasapteris rothwellii gen. et sp. nov., a Permineralized Fertile Pinnule of a Probable Stem Polypod from the Late Cretaceous of Hokkaido, Japan

Premise of research. The Late Cretaceous Yezo Group in Hokkaido, Japan, yields abundant plant debris in calcareous concretions. Nearly 150 species of plants have been described since 1910. Here we add a new genus of a probable stem polypodioid leptosporangiate fern to the plants of the Yezo Group based on a permineralized fertile pinnule from the Mikasa locality. Methodology. The fertile pinnule was detected in a calcareous concretion as an X-ray computed tomography image. Specimens were studied from serial cellulose acetate peels. Three-dimensional reconstructions of the entire pinnule and the soral, sporangial, and indusial morphologies were rendered using Molcer Plus and Amira. Pivotal results. The pinnule is 11.7 mm long, less than 7 mm wide, and deeply pinnatisect, with an abaxially grooved midrib or “costule” bearing one terminal and six lateral segments with catadromic pinnate venation. Lateral veins are simple or bifurcate once near the segment margin. The sorus is indusiate and abaxial and occurs slightly proximal to the end of the single vein or, when the lateral vein bifurcates, near the end of a much shorter anadromic vein. The indusium is reniform, is attached close to a massive receptacle only at its base, and is not laterally fused with the lamina. Approximately 17 sporangia with a slightly oblique, incomplete annulus, each estimated to contain ca. 64 spores, are formed per sorus. Spores are tetrahedral and trilete, have a triangular amb and slightly convex sides, are 42–45 μm in equatorial diameter, have a smooth exine, and are comparable to Biretisporites-type dispersed spores. Conclusions. The unique characters of the fossil are shared separately by extant basal polypods, most abundantly in Dennstaedtiaceae. However, the oblique annulus of the fossil does not occur in any extant members of Polypodiales unless it appears homoplastically. We therefore describe the fossil as a new taxon of a probable stem polypod, Mikasapteris rothwellii gen. et sp. nov., without any order and family assignments. The presence of Mikasapteris during the Late Cretaceous shows that pre-Polypodiales ferns were more diverse during this period.
Gaviss : Boosting the Performance of GPU-Accelerated NFV Systems via Data Sharing

GPUs have demonstrated the capability of significantly improving the performance of network functions (NF). In an Network Function Virtualization (NFV) system, multiple NFs form a service chain to provide services. However, NFs in state-of-the-art GPU-accelerated NFV systems still utilize a GPU independently where each NF needs to transfer data to the GPU memory for acceleration. As a result, a packet might be transferred into the GPU memory by each NF when it passes through the service chain. We find these expensive and repetitive transfers are the main factor that limits the overall performance of an NFV system. We propose Gaviss, a GPU-accelerated NFV system with effective data sharing. By sharing packets in the GPU memory among network functions, a packet needs to be transferred to the GPU only once, eliminating the performance overhead caused by repetitive transfers. Extensive experimental results show that Gaviss can improve the overall throughput by 2.6-13.2× and reduce the latency by up to 37.9%, when compared with state-of-the-art approaches. Moreover, Gaviss also demonstrates up to 2.5× higher price-performance ratio than CPU-based implementations, making GPUs competitive for building NFV systems.
Sample Selection Models Without Exclusion Restrictions: Parameter Heterogeneity and Partial Identification

This paper studies semiparametric versions of the classical sample selection model (Heckman (1976, 1979)) without exclusion restrictions. We extend the analysis in Honor´e and Hu (2020) by allowing for parameter heterogeneity and derive implications of this model. We also consider models that allow for heteroskedasticity and brieﬂy discuss other extensions. The key ideas are illustrated in a simple wage regression for females. We ﬁnd that the derived implications of a semiparametric version of Heckman’s classical sample selection model are consistent with the data for women with no college education, but strongly rejected for women with a college degree or more.
Effectiveness of Comirnaty® Vaccine and Correlates of Immunogenicity and Adverse Reactions: A Single-Center Prospective Case Series Study

The literature suggests that real-world data on the effectiveness and safety of the BNT162b2 vaccine depend on the characteristics of the vaccinated volunteers. The purpose of this study was to evaluate antibody responses and kinetics, established association with sociodemographic and clinical characteristics, and adverse reactions after complete vaccination with the BNT162b2 vaccine. A single-center prospective case series study was conducted with 112 eligible volunteers who were institutionalized elderly and health care workers with had a negative anti-SARS-CoV-2 IgG test prior to receiving the first dose of vaccine. At least one serological antibody test after each dose of vaccine was performed. Volunteers with a positive SARS-CoV-2 PCR test before vaccination were excluded. A chemiluminescent immunoassay anti-S1 antibody assay performed a serological evaluation. Both vaccine doses elicited positive IgG antibodies 3799.0 ± 2503.0 AU/mL and 8212.0 ± 4731.0 AU/mL after 20 days of the first and second doses of BNT162b2, respectively. Comirnaty® vaccine induced an immune response with antibody production against SARS-CoV-2 in 100% of participants, regardless of age (Spearman rho = −0.10, p-value = 0.312), body mass index (Spearman rho = 0.05, p-value = 0.640), blood group first dose (p-value for Kruskal–Wallis test = 0.093) and second dose (p-value for Kruskal–Wallis test = 0. 268), number of drugs (Spearman rho = −0.07, p-value = 0.490), and number of chronic diseases first dose (p-value for Kruskal–Wallis test = 0.632) and second dose (p-value for Kruskal–Wallis test = 0.510). IgG antibodies to SARS-CoV-2 were intensely elevated after the second administration of the BNT162b2 vaccine. The higher the titer of anti-peptide IgG antibodies generated after the first dose of vaccine, the higher the titer generated by the second dose of vaccine (Spearman rho = 0.86, p-value < 0.001) and the total antibody titer (Spearman rho = 0.93, p-value < 0.001). Furthermore, no serious adverse effects were reported among participants, although mild to moderate adverse effects (local or systemic) were reported after both doses of the BNT162b2 vaccine, being more frequent after the first dose of the vaccine. No participants showed a positive PCR. The BNT162b2 vaccine induces a robust and rapid antibody response regardless of participant characteristics. The second dose might be especially important because of the increased immunogenicity it produces and the possible temporal distancing of the interval between doses. In general, the vaccines were well tolerated.
The Impact of Cultural Heterogeneity on Violence in Indonesia: Fractionalisation versus polarization

ABSTRACT The determinants of large-scale conflict have been examined extensively in the academic literature. The factors contributing to everyday violence have received less attention despite this smaller-scale conflict having a high human and economic cost. We analyse a dataset at the level of 495 Indonesian districts to estimate the determinants of non-domestic, small-scale violence prevalent in this nation. We focus on the role of pronounced cultural heterogeneity that characterizes Indonesia and contribute to the literature by empirically testing whether established conflict theory holds for smaller scale violence data in the case of Indonesia. Ethnic polarization (rather than fractionalization) is the main driver of conflict intensity, suggesting that district-level conflicts are commonly over public goods. Cultural heterogeneity has a curvilinear effect on conflict. It increases the intensity of violence up to a point, after which the level decreases. Overall, our results offer some support for the Esteban and Ray model of conflict.
Cardiac Electrical Changes during Co-administration of Artemether-lumefantrine and Atazanavir/Ritonavir in HIV-infected Nigerian Adults

Background: Malaria and HIV infections are both major public health problems in Africa with significant morbidity and mortality. Atazanavir and artemether-lumefantrine are used in the treatment of HIV and Malaria respectively which are co-administered when there is co-infection with both diseases. Both drugs are known to independently cause cardiac electrical abnormalities. In addition, atazanavir inhibits CYP3A4 which is the enzyme that metabolizes lumefantrine with the potential for increased cardiac electrical abnormalities. We, therefore, assessed the cardiac electrical activities during the co-administration of atazanavir and artemether-lumefantrine. Method: Forty-one consecutive HIV-infected adults attending the Anti-retroviral therapy clinic in a Nigeria General hospital were enrolled on the study using the convenience sampling technique. Study participants had electrocardiographic tracings done before (day 0) and after treatment with artemether/lumefantrine (days 3 and 7). Result: There was an increase in the mean heart rate from day 1 through day 7 but the mean heart rates were within normal limits. In contrast, there was a progressive decline in the mean PR interval from day 0 through day 7 which was not statistically significant. There was also a significant increase in corrected QT interval (QTc) between day 0 and day 3 and day 0 and day 7. Though the value of the mean QTc on day 3 (414.83±34.88ms) and day 7 (411.41 ± 22.63ms) remained within the normal limit, 5% (2) of the participant had prolonged QTc. Conclusion: The results of this study suggested that there may be potential drug-drug interaction resulting from the co-administration of atazanavir based antiretroviral drug and artemether-lumefantrine especially in the prolongation of QTc interval.