Miracle-dz/UniFAR-FTUs huggingface dataset:
Midwifery Benchmarking in Oregon: Increasing Participation to Deliver Change

Benchmarking is an evidence based method of quality improvement (QI) supported by the literature (Anand & Kodali, 2008; Yasin, 2002). The ACNM Benchmarking Project has been in existence since 2004, but has struggled with increasing member participation, ultimately limiting the strength of the produced data (Collins-Fulea et al., 2005; Walker et al., 2008). This project aimed to assess current benchmarking practices in Oregon, identify individual practices for benchmarking assistance, and increase Oregon participation in the national project. Oregon ACNM members were surveyed and found widespread agreement in the value of QI and benchmarking awareness. The greatest barriers cited, regardless of years submitting data, were time and health system’s issues such as data collection and practice structure. Seven practices requesting practice-level assistance were identified and contacted and provided with the help, education, and reminders they needed. Thirteen practices participated this year, an increase from the ten practices last year. This project’s noted strengths were quality improvement initiative among Oregon nurse-midwives and an initial assessment that can be used for future projects. Recommendations include increasing publication of success stories related to application of benchmarking data for practice improvement or advancement; reducing practice-level barriers to data collection by creating an easily accessible list of best practice for data collection; and lastly, utilization of QI efforts to drive progress in midwifery care. MIDWIFERY BENCHMARKING IN OREGON: DELIVERING CHANGE 3 Midwifery Benchmarking in Oregon: Increasing Participation to Deliver Change The American College of Nurse-Midwives (ACNM) is the professional association representing Certified Nurse-Midwives (CNMs) and Certified Midwives (CMs) in the United States. The mission of ACNM is to support midwives and advance the practice of midwifery in order to achieve optimal health for women through their lifespan. This mission recognizes the profession’s expertise in well-woman and gynecologic care, promotion of optimal pregnancy, physiologic birth, postpartum care, and care of the newborn. The ACNM mission is realized in part through the core value of evidence-based practice and use of quality measurement to improve care. A product of that value is the ACNM Benchmarking Project, a tool to collect aggregate clinical data to determine midwifery-based quality assurance. Additionally, the benchmarking project produces accurate and tangible evidence of improved outcomes and lower costs of midwifery care on a national level. Lastly, this information can be utilized to advocate for policy and clinical practice change. Data collected by the ACNM benchmarking project provides a unique opportunity to solidify the profession of midwifery as a key stakeholder in the current initiatives to improve national health care.
Wafer-scale graphene quality assessment using micro four-point probe mapping

Micro four-point probes (M4PP) provide rapid and automated lithography-free transport properties of planar surfaces including two-dimensional materials. We perform sheet conductance wafer maps of graphene directly grown on a 100 mm diameter SiC wafer using a multiplexed seven-point probe with minor additional measurement time compared to a four-point probe. Comparing the results of three subprobes we find that compared to a single-probe result, our measurement yield increases from 72%–84% to 97%. The additional data allows for correlation analysis between adjacent subprobes, that must measure the same values in case the sample is uniform on the scale of the electrode pitch. We observe that the relative difference in measured sheet conductance between two adjacent subprobes increase in the transition between large and low conductance regions. We mapped sheet conductance of graphene as it changed over several weeks. Terahertz time-domain spectroscopy conductivity maps both before and after M4PP mapping showed no significant change due to M4PP measurement, with both methods showing the same qualitative changes over time.
Bovine Bone Promotes Osseous Protection via Osteoclast Activation

The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL/6 mouse alveolar regeneration bone model (n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin/eosin and tartrate-resistant acid phosphatase staining. A separate group (n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group (n = 28) for examination of osseous changes and cellular functions with micro–computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts’ function. Osteoclasts’ inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts’ inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.
Bacteriophage Based Biosensors: Trends, Outcomes and Challenges

Foodborne pathogens are one of the main concerns in public health, which can have a serious impact on community health and health care systems. Contamination of foods by bacterial pathogens (such as Staphylococcus aureus, Streptococci, Legionella pneumophila, Escherichia coli, Campylobacter jejuni and Salmonella typhimurium) results in human infection. A typical example is the current issue with Coronavirus, which has the potential for foodborne transmission and ruling out such concerns is often difficult. Although, the possible dissemination of such viruses via the food chain has been raised. Standard bacterial detection methods require several hours or even days to obtain the results, and the delay may result in food poisoning to eventuate. Conventional biochemical and microbiological tests are expensive, complex, time-consuming and not always reliable. Therefore, there are urgent demands to develop simple, cheap, quick, sensitive, specific and reliable tests for the detection of these pathogens in foods. Recent advances in smart materials, nanomaterials and biomolecular modeling have been a quantum leap in the development of biosensors in overcoming the limitations of a conventional standard laboratory assay. This research aimed to critically review bacteriophage-based biosensors, used for the detection of foodborne pathogens, as well as their trends, outcomes and challenges are discussed. The future perspective in the use of simple and cheap biosensors is in the development of lab-on-chips, and its availability in every household to test the quality of their food.
The Oral Archaeome: A Scoping Review

The Archaea domain was recognized as a separate phylogenetic lineage in the tree of life nearly 3 decades ago. It is now known as part of the human microbiome; however, given that its roles in oral sites are still poorly understood, this review aimed to establish the current level of evidence regarding archaea in the oral cavity to guide future research, providing insights on the present knowledge about the human oral archaeome. A scoping review was conducted with the PRISMA Extension for Scoping Reviews checklist. Five electronic databases were searched, as well as gray literature. Two independent reviewers performed the selection and characterization of the studies. Clinical studies were included when the target population consisted of humans of any age who were donors of samples from the oral cavity. A qualitative analysis was performed, based on the type of oral site and by considering the methods employed for archaeal identification and taxonomy, including the DNA extraction protocols, primers, and probes used. Fifty articles were included in the final scoping review, published from 1987 to 2019. Most studies sampled periodontal sites. Methanogens were the most abundant archaea in those sites, and their presence could be associated with other periodontal pathogens. No consistent relationship with different disease conditions was observed in studies that evaluated the microbiota surviving in endodontic sites. Few articles analyzed the presence of archaea in dental caries, saliva, or tongue microbiota, as well as in archaeologic samples, also showing a relationship with healthy microbiota. Archaea have been detected in different oral niches of individuals from diverse geographic locations and clinical conditions, suggesting potential roles in oral diseases. Methodological limitations may hamper our current knowledge about archaeal diversity and prevalence in oral samples, and future research with diversified methodological approaches may lead to a better comprehension of the human oral archaeome.
Oncology nurses' experiences of meeting with men with cancer-related fatigue: a qualitative study.

BACKGROUND Cancer treatment often causes side effects, among which fatigue is common and can persist for years among disease-free cancer survivors. Living with fatigue can lead to reduced life expectancy and quality of life. AIMS AND OBJECTIVES To describe oncology nurses' experiences of meeting with men with cancer and talking about cancer-related fatigue. DESIGN AND METHODS The data were collected via semi-structured interviews with nine oncology nurses recruited using a purposeful sampling method and analysed using thematic content analysis. This qualitative design was conducted to describe and interpret the content of experiences. ETHICAL ISSUES AND APPROVAL The study was approved by a regional Ethical Review Board, and research ethical principles were followed. RESULTS The analysis revealed one major theme, namely take the whole person into consideration, and three subthemes: the importance of (i) establishing trust in the nurse-patient relationship, (ii) supporting the patient's understanding of cancer-related fatigue, and (iii) managing the challenging emotions experienced by patients. The major theme describes the oncology nurses' approach when meeting with men with cancer-related fatigue. CONCLUSION The results provide insight into how oncology nurses can increase their knowledge about fatigue to establish trust in nurse-patient relationships. They can acquire knowledge about how to make men with cancer-related fatigue feel safe when handling their daily lives despite their fatigue. Oncology nurses require knowledge, presence and commitment in their interactions with men with cancer-related fatigue to be able to take the whole person into consideration. This study demonstrates the importance of the approach oncology nurses take when interacting with men with cancer-related fatigue and the strategies required when talking about cancer-related fatigue.
Suicide amongst the Inuit of Nunavut: An Exploration of Life Trajectories

This article reports results of the life trajectories from 92 Inuit who died by suicide, matched for age and gender with 92 living-controls. A proxy-based procedure and semi-structured interviews with informants were conducted to obtain trajectories of developmental events occurring over the life course for suicide and community-matched controls. Results from this research indicate two different trajectories that differentiate the control-group from the suicide-group throughout the life course. Even though the number of suicide attempts are similar between both groups, the suicide-group had a more important burden of adversity, which seemed to create a cascading effect, leading to suicide.
Subsecond EEG-fMRI analysis for presurgical evaluation in focal epilepsy.

OBJECTIVE The authors recently reported a novel subsecond analysis method of analyzing EEG-functional MRI (fMRI) to improve the detection rate of epileptic focus. This study aims to validate the utility of this method for presurgical evaluation in pharmacoresistant focal epilepsy. METHODS Among 13 patients with focal epilepsy undergoing presurgical examinations including simultaneous EEG-fMRI at 3T, 11 patients had interictal epileptiform discharges (IEDs) during fMRI. The authors used the sequence of topographic maps during the IEDs as a reference to obtain subsecond fMRI activation maps with the same temporal resolution as the EEG data, and constructed "spike-and-slow-wave-activation-summary" (SSWAS) maps that showed the activation frequency of voxels during IEDs. Clusters were defined by thresholding the SSWAS maps (voxel value > 10), and those containing voxels with the top 3 highest activation frequencies were considered significant. Significant hemodynamic responses using conventional event-related (ER) analysis and SSWAS maps were compared with the resection areas and surgical outcomes at 1 year after surgery. RESULTS Using ER analysis, 4 (36%) of 11 patients had significant hemodynamic responses. One of 4 patients had significant hemodynamic responses in the resection area and good surgical outcome. Using SSWAS maps, 10 (91%) of 11 patients had significant hemodynamic responses. Six of 10 patients had significant hemodynamic responses in the resection area, and 5 of the 6 patients achieved good surgical outcomes. The remaining 4 patients had significant hemodynamic responses distant from the resection area, and only 1 of the 4 patients achieved good surgical outcomes. The sensitivity, specificity, positive predictive value, and negative predictive value of SSWAS maps were 83.3%, 75.0%, 83.3%, and 75.0%, respectively. CONCLUSIONS This study demonstrated the clinical utility of SSWAS maps for presurgical evaluation of pharmacoresistant focal epilepsy. The findings indicated that subsecond EEG-fMRI analysis may help surgeons choose the resection areas that could lead to good surgical outcomes.
The exercise pressor reflex and chemoreflex interaction: cardiovascular implications for the exercising human

Although the exercise pressor reflex (EPR) and the chemoreflex (CR) are recognized for their sympathoexcitatory effect, the cardiovascular implication of their interaction remains elusive. We quantified the individual and interactive cardiovascular consequences of these reflexes during exercise and revealed various modes of interaction. The EPR and hypoxia‐induced CR interaction is hyper‐additive for blood pressure and heart rate (responses during co‐activation of the two reflexes are greater than the summation of the responses evoked by each reflex) and hypo‐additive for peripheral haemodynamics (responses during co‐activation of the reflexes are smaller than the summated responses). The EPR and hypercapnia‐induced CR interaction results in a simple addition of the individual responses to each reflex (i.e. additive interaction). Collectively, EPR:CR co‐activation results in significant cardiovascular interactions with restriction in peripheral haemodynamics, resulting from the EPR:CR interaction in hypoxia, likely having the most crucial impact on the functional capacity of an exercising human.
The Incidence and Nature of Adverse Medical Device Events in Dutch Hospitals: A Retrospective Patient Record Review Study

Supplemental digital content is available in the text. Objectives Despite widespread use of medical devices and their increasing complexity, their contribution to unintended injury caused by healthcare (adverse events, AEs) remains relatively understudied. The aim of this study was to gain insight in the incidence and types of AEs involving medical devices (AMDEs). Methods Data from two patient record studies for the identification of AEs were used. Identification of AMDEs was part of these studies. Patient records of 6894 admissions of a random sample of 20 hospitals in 2011/2012 and 19 hospitals in 2015/2016 were reviewed for AMDEs by trained nurses and physicians. Results In 98.7% of the admissions, a medical device was used. Adverse events involving medical devices were present in 2.8% of the admissions, with 24% of the AMDEs being potentially preventable. Of all AEs, in 40%, medical devices were involved. Of all potentially preventable AEs, in 44%, medical devices were involved. Implants were most often involved in potentially preventable AMDEs. Conclusions Medical devices are substantially involved in potentially preventable AEs in hospitals. Research into AMDEs is of great importance because of the increasing use and complexity of medical devices. Based on patient records, most improvements could be made for placement of implants and prevention of infections related to medical devices. Safety and safe use of medical devices should be a subject of attention and further research.
Community response to hiv/aids: a case of pastoralists in Kilosa district, Tanzania .

A study to assess the response of pastoralist community towards HIV/AIDS was conducted in two villages of Kilosa district in Morogoro region. A cross sectional research design was adopted whereby a purposive sampling technique was used in combination with simple random sampling method so as to obtain a sample of 51 adult respondents and snow-ball technique was used to obtain 42 youth respondents. In addition 20 key informants were sampled. A structured questionnaire was the main instrument used in data collection. Checklists were used for key informants and focus group discussions (FGDs). The data were statistically analyzed using SPSS computer programme to obtain frequencies and percentages. Results have shown that all respondents were aware of the HIV/AIDS pandemic. More than 90% showed positive attitudes towards HIV/AIDS and PLWHA. Education level was observed to be statistically associated with HIV/AIDS testing status at p<0.05. Individuals with primary education were more likely to go for HIV testing than those with no formal education. Also the study observed negative and positive responses shown by individuals and households towards HIV/AIDS. Negative responses observed were: taking children out of school, sending children to relatives, selling of productive assets (livestock) for food and medical costs, skipping some meals and depending on wild food. The positive observed responses included asking for loans, selling labour and move to crop farming. Generally, response by civil society organizations (CSOs) were observed to be positive such as care and support for infected and affected individuals and households by providing material support such as monetary, food, clothes, medical care and medicine, moral or spiritual support, guidance in counselling and testing for HIV/AIDS and mitigation of HIV/AIDS. Central government responses were also very positive such as educating the community on HIV/AIDS, care and support for PLWHA. ii
The antinociceptive effects of a dual kappa-delta opioid receptor agonist in the mouse formalin test

Pain management is a challenging and unmet medical need. Despite their demonstrated efficacy, currently used opioid drugs and nonsteroidal anti-inflammatory drugs are frequently associated with several adverse events. The identification of new and safe analgesics is therefore needed. MP1104, an analogue of 3′−iodobenzoyl naltrexamine, is a potent nonselective full agonist at mu (MOR), kappa (KOR), and delta (DOR) opioid receptors, respectively. It was shown to possess potent antinociceptive effects in acute thermal pain assays without aversion in mice. In this study, we investigated MP1104 in the formalin test, a model of tonic pain. MP1104 (0.05, 0.1, and 1.0 mg/kg) reduced pain-like behaviors in phases I and II of the formalin test in male and female ICR mice. Pretreatment with KOR antagonist (norbinaltorphimine 10 mg/kg) and DOR antagonist (naltrindole 10 mg/kg) abolished the antinociceptive effects of MP1104 in the formalin test. These findings support the development of MP1104 for further testing in other pain models.
Identification and Genome Analysis of Vibrio coralliilyticus Causing Mortality of Pacific Oyster (Crassostrea gigas) Larvae

Vibrio coralliilyticus is known as a coral pathogen that also infects marine bivalve larvae worldwide. It is considered to be one of the major constraints in artificial marine bivalve seed production as it causes mortality. In this study, we first isolated and characterized a high virulent of V. coralliilyticus designated as SNUTY-1 that was the cause of Pacific oyster larvae mortality in Korea. In the pathogenicity test, exposure to 2.14 × 105 CFU/mL for 24 h caused mortality to 88.65 ± 2.4% of the tested healthy Pacific oyster larvae. SNUTY-1 showed anti-microbial resistance to β-lactams, such as penicillins, cephalosporins, and carbapenems. We sequenced and assembled the complete genome of SNUTY-1 (5,842,676 bp), consisting of two chromosomes (Chr I and Chr II) and two plasmids (pSNUTY1 and pSNUTY2). The COG functional analysis confirmed that Chr I had more genes associated with basic cellular functions in comparison to Chr II. The results of the phylogenetic trees based on OrthoANI values indicated that the SNUTY-1 was closely related to V. coralliilyticus strains. SNUTY-1 had a unique plasmid (pSNUTY2), which could mean that the Korean isolate is different from other sequenced V. coralliilyticus strains from different geographical origins. Toxic proteins such as cytolysin/hemolysin and extracellular metalloprotease genes were encoded on Chr I and Chr II of SNUTY-1. These data facilitate the control of V. coralliilyticus infections in aquaculture by providing valuable insights into the biodiversity of this organism and valuable information for the study of virulence factors.
Identification of the Core Pollen-Specific Regulation in the Rice OsSUT3 Promoter

The regulatory mechanisms of pollen development have potential value for applications in agriculture, such as better understanding plant reproductive regularity. Pollen-specific promoters are of vital importance for the ectopic expression of functional genes associated with pollen development in plants. However, there is a limited number of successful applications using pollen-specific promoters in genetic engineering for crop breeding and hybrid generation. Our previous work led to the identification and isolation of the OsSUT3 promoter from rice. In this study, to analyze the effects of different putative regulatory motifs in the OsSUT3 promoter, a series of promoter deletions were fused to a GUS reporter gene and then stably introduced into rice and Arabidopsis. Histochemical GUS analysis of transgenic plants revealed that p385 (from −385 to −1) specifically mediated maximal GUS expression in pollen tissues. The S region (from −385 to −203) was the key region for controlling the pollen-specific expression of a downstream gene. The E1 (−967 to −606), E2 (−202 to −120), and E3 (−119 to −1) regions enhanced ectopic promoter activity to different degrees. Moreover, the p385 promoter could alter the expression pattern of the 35S promoter and improve its activity when they were fused together. In summary, the p385 promoter, a short and high-activity promoter, can function to drive pollen-specific expression of transgenes in monocotyledon and dicotyledon transformation experiments.