Miracle-dz/UniFAR-FTUs huggingface dataset:
Defensive Projection, Superimposed On Simplistic Object Relations, Erodes Patient-Provider Relationships in High-Risk Pregnancy: an Empirical Investigation

In an attempt to illustrate the relevance of psychoanalytic theory and research to behavior medicine, an empirical investigation was conducted of females treated at a high-risk pregnancy specialty clinic (N = 58). Drawing from psychoanalytic object relations theory, it was hypothesized and confirmed that use of projection as a defense mechanism during pregnancy, superimposed on simplistic object relations, predicted an erosion of patient-provider relationships during the pregnancy/postdelivery period. Findings are interpreted through the perspective of mentalization, pertaining to individuals’ ability to understand the mental states of self and others, specifically under significant stress. Implications for psychoanalytically oriented assessment and treatment, and for the rift between psychoanalysis and research, are discussed.
Functional Heterogeneity within Rat Orbitofrontal Cortex in Reward Learning and Decision Making

Rat orbitofrontal cortex (OFC) is located in the dorsal bank of the rhinal sulcus, and is divided into the medial orbital area, ventral orbital area, ventrolateral orbital area, lateral orbital area, dorsolateral orbital area, and agranular insular areas. Over the past 20 years, there has been a marked increase in the number of publications focused on the functions of rat OFC. While collectively this extensive body of work has provided great insight into the functions of OFC, leading to theoretical and computational models of its functions, one issue that has emerged relates to what is defined as OFC because targeting of this region can be quite variable between studies of appetitive behavior, even within the same species. Also apparent is that there is an oversampling and undersampling of certain subregions of rat OFC for study, and this will be demonstrated here. The intent of the Viewpoint is to summarize studies in rat OFC, given the diversity of what groups refer to as “OFC,” and to integrate these with the findings of recent anatomical studies. The primary aim is to help discern functions in reward learning and decision-making, clearing the course for future empirical work.
Analysis of web content delivery effectiveness and efficiency in responsive web design using material design guidelines and User Centered Design

Nowadays, websites are not only be accessed by computers with a large screen. They were mostly accessed via mobile devices such as tablets and smartphones that relatively have smaller screen size. Almost every website has their different style and visual appearance concerning content and information delivery. Even though they were visually looked different, they were mostly designed and developed by using one of one column, two columns, or three columns layout. The way to properly deliver content and information on a smaller screen size becomes a challenging matter. Responsive Web Design approach allows a single web page to be differently visualized based on the screen size of the accessing device. Such layout changing may affect the amount of information to be displayed on the screen, hence affecting the effectiveness and efficiency of information delivery in a web page. This study compares the effectiveness and efficiency of a web page that is being displayed on a computer screen, tablet, and smartphones. How the implementation of Materials Design Guidelines and User-Centered Design approaches in the design process may affect the effectiveness and efficiency of content and information delivery of a web page on smaller screens were also evaluated and presented. This research shows that User Centered Design and Material Design Guidelines improve the effectiveness and efficiency of content delivery on both tablet and smartphone. Materials Design Guidelines provide better usability improvement than User Centered Design approach for the smartphone screen regarding content delivery efficiency for web pages designed in three columns layout.
Contact based robot control through tactile maps

This paper deals with the problem of controlling a robot during a physical interaction through a representation of its tactile system. Although the force feedback has been widely used to control the interaction with objects (e.g., object grasping and manipulation), the use of tactile data in more complex interaction tasks did not receive a comparable attention. The lack of an available skin-like technology has limited the development of tactile based control strategies. The major contribution of this paper is to describe a control strategy controlling the interaction task of a robot arm, equipped with tactile sensors, in contact with an external object or human through the somatosensory map. Experiments are used to validate the approach.
Differential Action of Methamphetamine on Tyrosine Hydroxylase and Dopamine Transport in the Nigrostriatal Pathway of μ-Opioid Receptor Knockout Mice

ABSTRACT Extensive anatomical and functional interactions exist between central dopaminergic and opioidergic systems and both systems are proposed to be targets for amphetamine-like drugs. We have previously reported that μ-opioid receptor (μ-OR) knockout mice are resistant to the loss of dopamine in the striatum and the development of behavioral sensitization induced by repeated methamphetamine (METH) treatment. The present study assessed whether METH-treated μ-OR knockout mice exhibit a differential response of the expression of dopamine transporter and tyrosine hydroxylase (TH), the rate-limiting enzyme for dopamine synthesis and maintaining dopamine levels. Mice daily received intraperitoneal injection of METH (0, 0.6, 2.5, or 10 mg/kg) for 7 days and sacrificed on day 11 (4 days after the last injection). The expression of TH protein in the striatum and the levels of TH mRNA and number of TH positive neurons in the substantia nigra were reduced in wild-type mice treated with METH (2.5 and 10 mg/kg), but not in the μ-OR knockout mice. In contrast, METH exposure at the highest dose (10 mg/kg) reduced dopamine transporter levels in both strains of mice. These results suggest that the μ-OR contributes to METH-induced loss of dopamine and behavioral sensitization by decreasing the expression of TH.
Protein Sam68 regulates the alternative splicing of survivin DEx3

Messenger RNA alternative splicing (AS) regulates the expression of a variety of genes involved in both physiological and pathological processes. AS of the anti-apoptotic and proliferation-associated survivin (BIRC5) gene generates six isoforms, which regulate key aspects of cancer initiation and progression. One of the isoforms is survivin DEx3, in which the exclusion of exon 3 generates a unique carboxyl terminus with specific anti-apoptotic functions. This isoform is highly expressed in advanced stages of breast and cervical tumors. Therefore, understanding the mechanisms that regulate survivin DEx3 mRNA AS is clearly important. To this end, we designed a minigene (M), and in combination with a series of deletions and site-directed mutations, we determined that the first 22 bp of exon 3 contain cis-acting elements that enhance the exclusion of exon 3 to generate the survivin DEx3 mRNA isoform. Furthermore, using pulldown assays, we discovered that Sam68 is a possible trans-acting factor that binds to this region and regulates exon 3 splicing. This result was corroborated using a cell line in which the Sam68 binding site in the survivin gene was mutated with the CRISPR/Cas system. This work provides the first clues regarding the regulation of survivin DEx3 mRNA splicing.
Microcontroller-based backlight module with DPLL function for reducing the parasitic capacitance effect in LCD panel housing

A microcontroller-based backlight module is proposed to reduce the leakage current effect in LCD. First, the mathematical model of the parasitical capacitance in LCD panel is conducted to explore the influence of leakage current effect. Moreover, a class D resonant backlight inverter is employed to act as the main circuit. Next, the phase angle variations caused by the parasitic capacitances is considered as a reference parameter in the proposed control strategy. By using the primary-side control and incorporating the DPLL function programmed by software to form a feedback mechanism to track the optimal operating frequency. And then the influence of parasitic capacitance can be reduced so as to eliminate the leakage current effect, hence, the system efficiency and stability will be improved. Complete mathematical analysis and design considerations are detailed. Experimental results agree with the theoretical predictions.
Study of hepatitis C virus genotype in Guizhou area of southwestern China.

OBJECTIVE To determine the concordance in various hepatitis C (HCV) genotyping methods and to investigate the distribution of HCV genotypes in Guizhou area of Southwest China. METHODS Serum samples from 206 patients (100 with chronic hepatitis and 106 with hemopathy) were detected for antibody of HCV by second generation enzyme-labelled immunosorbent assay (ELISA). Thirty-five anti-HCV positive samples were detected for HCV RNA by RT-polymerase chain reaction (PCR) and 30 HCV RNA positive samples were determined for their genotypes by three various genotyping methods [PCR with type-specific primers at the core region (primer-set), slot-blot hybridization with type-specific probes at NS5B region (blotting) and the restric fragment length polymorphism analysis of PCR products of 5' NC region (RFLP)]. Ten samples with the known genotype were analysed by the direct sequencing. RESULTS Of 30 samples with positive HCV RNA, the types of 22 could be classified by three methods, and the genotypes determined by various methods had complete concordance. The types of 6 samples could be classified by two methods and 5 had agreement subtypes. The types of two samples could be classified only by RFLP. Overall, 27 (90.0%) had subtype 1b infection and 3 (10.0%) had subtype 2a infection. The nucleotide sequence of 8 samples with subtype 1b and one with subtype 2a were analysed by the direct sequencing. The subtypes determined by sequence analysis were in complete concordance with those decided by various genotyping methods. CONCLUSIONS Subtype 1b is the predominent HCV genotype in Guizhou area, while subtype 2a is less common. There was a good concordance with the genotyping results obtained by various HCV genotyping methods.
On a battery/supercapacitor powered SRM drive for EV with integrated on-board charger

This paper develops a battery/supercapacitor powered switched-reluctance motor (SRM) drive for electric vehicle (EV) propulsion. Its on-board integrated charger is formed using the embedded motor drive components. The boosted motor drive DC-link voltage is established by an interleaved DC/DC boost converter from the battery. The super-capacitor (SC) is interfaced to the DC-link via a DC/DC buck converter. And it is connected to the battery bank by a diode to assist the battery in acceleration and deceleration. Good driving and regenerative braking performances are achieved by proper control. In idle condition, the grid-to-vehicle (G2V) battery charging can be conducted by the formed on-board integrated charger with good line drawn power quality. The established charger consists of a full-bridge boost switch-mode rectifier (SMR) and a buck DC/DC converter based charger.
Regulation of type 2 nitric oxide synthase by type 1 interferons in macrophages infected with Leishmania major

We recently reported that the infection of macrophages with Leishmania major led to the release of type 1 interferons (IFN‐α /β ). Moreover, at day 1 of infection of mice with L. major, IFN‐α /β was required for the expression of type 2 (inducible) NO synthase (NOS2 or iNOS) which, however, was restricted to a few macrophages in the dermis. Here, we further characterized the regulation of NOS2 by IFN‐α /β . Macrophages that were either simultaneously or sequentially exposed to L. major promastigotes and IFN‐α /β expressed NOS2 and anti‐leishmanial activity. In contrast, when high amounts of IFN‐α /β were used or when IFN‐α /β was added to the macrophages 2 h prior to the parasites, almost no induction of NOS2 was observed. After pretreatment with IFN‐α /β , tyrosine phosphorylation and nuclear DNA binding of Stat1α, the degradation of the NF‐κB inhibitor (IκBα and β), and the nuclear translocation of NF‐κB were strongly impaired compared with macrophages exposed to IFN‐α /β and L. major simultaneously. Thus, IFN‐α /β exerts agonistic or antagonistic effects on the expression of NOS2 in macrophages infected with a microbial pathogen, depending on the sequence of the stimuli and the amount of IFN‐α /β added. The limited number of NOS2‐positive macrophages at day 1 of infection in vivo might result from a blockage of non‐infected macrophages by IFN‐α /β that is released by neighboring infected cells.
Stable Adaptive Teleoperation

Telerobotics, the body of science and technology which bridges human control and purely autonomous machines, is expected to be a merging point of modern developments in robotics, control theory, cognitive science, machine design, and computer science. Besides traditional applications in space, subsea, and handling of hazardous material, many new potential uses of advanced telerobotic systems have recently been suggested or explored, such as safety applications or microsurgery. This paper is a preliminary study of what role recent results in adaptive robot control and the understanding of time-delays may play in the development of effective telerobotics systems, which would best exploit the presence of the human operator while making full use of available robot control technology and computing power. The key paradigm it explores is that of simplifying, transforming, or enhancing the remote dynamics perceived by the operator by proper use of adaptive robot control techniques and tools from passivity theory.
EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS.

Some properties of a complement-fixing (CF) antigen for Eaton pleuropneumonia-like organism (PPLO) (Mycoplasma pneumoniae) are described. CF antigen was found to be heat stable and extractable with organic solvents. The properties of the antigen indicated that it either was lipid or was associated with lipid. Raw antigen was extremely anticomplementary (AC). AC activity of whole antigen could be reduced by boiling, while AC activity of solvent-soluble antigens was low. Antigen extracted by either chloroform or ether behaved as a complete antigen by eliciting antibody production in rabbits, while antigen produced by chloroformethanol-aqueous KCl partition (L antigen) was at best poorly immunogenic. For diagnostic purposes L antigen was more specific and demonstrated larger antibody responses than either boiled antigen or ether-extracted antigen. Testing of 40 paired sera from Eaton PPLO isolation-positive patients revealed 36 with 4-fold antibody titer rises, while the 4 others had high antibody in the first sera obtained 7 or more days after onset of illness. Geometric mean titer of first sera was 1:2.3 and of second sera 1:48, indicating an average 20-fold antibody response. Only 12 of 364 (3%) other patients with similar acute respiratory disease who were negative for Eaton PPLO isolation showed 4-fold antibody titer rises with L antigen.
Bacterial display in combinatorial protein engineering

Technologies for display of recombinant protein libraries are today essential tools in many research‐intensive fields, such as in the drug discovery processes of biopharmaceutical development. Phage display is still the most widely used method, but alternative systems are available and are becoming increasingly popular. The most rapidly expanding of the alternative systems are the cell display‐based technologies, offering innovative strategies for selection and characterization of affinity proteins. Most investigations have focused on eukaryotic yeast for display of protein libraries, but similar systems are also being developed using prokaryotic hosts. This review summarizes the field of bacterial surface display with a strong emphasis on library applications for generation of new affinity proteins. The main focus will be on the most recent progress of the work on primarily Escherichia coli, but also on studies using a recently developed system for display on Gram‐positive Staphylococcus carnosus. In addition, general strategies for combinatorial protein engineering using cell display are discussed along with the latest developments of new methodologies with comparisons to mainly phage display technology.
A MAS NMR Study of the Bacterial ABC Transporter ArtMP

ATP‐binding cassette (ABC) transport systems facilitate the translocation of substances, like amino acids, across cell membranes energised by ATP hydrolysis. This work describes first structural studies on the ABC transporter ArtMP from Geobacillus stearothermophilus in native lipid environment by magic‐angle spinning NMR spectroscopy. The 2D crystals of ArtMP and 3D crystals of isolated ArtP were prepared in different nucleotide‐bound or ‐unbound states. From selectively 13C,15N‐labelled ArtP, several sequence‐specific assignments were obtained, most of which could be transferred to spectra of ArtMP. Residues Tyr133 and Pro134 protrude directly into the ATP‐binding pocket at the interface of the ArtP subunits, and hence, are sensitive monitors for structural changes during nucleotide binding and hydrolysis. Distinct sets of NMR shifts were obtained for ArtP with different phosphorylation states of the ligand. Indications were found for an asymmetric or inhomogeneous state of the ArtP dimer bound with triphosphorylated nucleotides. With this investigation, a model system was established for screening all functional states occurring in one ABC transporter in native lipid environment.
Differential protein adduction by seven organophosphorus pesticides in both brain and thymus

There is a need for mechanistic understanding of the lasting ill health reported in several studies of workers exposed to organophosphorus (OP) pesticide. Although the acute toxicity is largely explicable by acetylcholinesterase inhibition and the lasting effects of frank poisoning by direct excitotoxicity or indirect consequences of the cholinergic syndrome, effects at lower levels of exposure would not be predicted from these mechanisms. Similarly, reversible interactions with nicotinic and muscarinic receptors in adults would not predict continuing ill health. Many OP pesticides produce protein adduction, and the lasting nature of this makes it a candidate mechanism for the production of continuing ill health. We found significant adduction of partially characterized protein targets in both rat brain and thymus by azamethiphos, chlorfenvinphos, chlorpyrifos—oxon, diazinon—oxon, dichlorvos and malaoxon, in vitro and pirimiphos-methyl in vivo. The diversity in the adduction pattern seen across these agents at low dose levels means that any longer term effects of adduction would be specific to specific organophosphates, rather than generic. This presents a challenge to epidemiology, as most exposures are to different agents over time. However, some adducted proteins are also expressed in blood, notably albumin, and so may provide exposure measures to increase the power of future epidemiological studies. Human & Experimental Toxicology (2007) 26, 347—353
The Adjacency Matrix of One Type of Directed Graph and the Jacobsthal Numbers and Their Determinantal Representation

Recently there is huge interest in graph theory and intensive study on computing integer powers of matrices. In this paper, we consider one type of directed graph. Then we obtain a general form of the adjacency matrices of the graph. By using the well-known property which states the (𝑖,𝑗) entry of 𝐴𝑚 (𝐴 is adjacency matrix) is equal to the number of walks of length 𝑚 from vertex 𝑖 to vertex 𝑗, we show that elements of 𝑚th positive integer power of the adjacency matrix correspond to well-known Jacobsthal numbers. As a consequence, we give a Cassini-like formula for Jacobsthal numbers. We also give a matrix whose permanents are Jacobsthal numbers.
Functional expression of the glycine transporter 1 on bullfrog retinal cones

Using patch clamp techniques, we characterized glycine-induced currents from cones in bullfrog retinal slices. Application of glycine to cone terminals induced an inward current, which was in part suppressed by strychnine. The remaining strychnine-resistant current component, which did not show polarity reversion in a range of −120 mV to +40 mV, was blocked by N[3-(4′-fluorophenyl)-3-(4′-phenylphenoxy)propyl] sarcosine, an antagonist of glycine transporter 1 (GlyT1), but not affected by amoxapine, an inhibitor of glycine transporter 2. Application of sarcosine, an agonist of GlyT1, to cone terminals induced an inward current that was completely suppressed by N[3-(4′-fluorophenyl)-3-(4′-phenylphenoxy)propyl] sarcosine or when external Na+ in Ringer's was replaced by choline. All these results show for the first time the functional expression of GlyT1 on bullfrog cones.
Facial beauty prediction model based on self-taught learning and convolutional restricted Boltzmann machine

The research of facial beauty mostly focuses on geometric features, which may easily lose much feature information characterizing facial beauty and rely heavily on the accurate manual localization of landmark facial features. Therefore, a novel method to extract apparent features of face images by convolutional restricted Boltzmann machine (CRBM) without relying on artificial features selection is proposed. Massive beautiful and ugly training samples are required by traditional machine learning methods, and it is hard to be satisfied because most face images are actually neutral beauty. A better method of relaxing strict restrictions of training samples is self-taught learning, which automatically improves CRBM to understand the characteristics of data distribution even if the requirements of the class and number of training samples are not satisfied, thus the facial beauty prediction model could be established reasonably. Experimental results show that the proposed facial beauty prediction model can achieve recognition rate of 87.3% on three classes of beautiful, ordinary and unbeautiful face images, and 95% on two classes of beautiful and unbeautiful face images. Meanwhile, the extracted apparent features can effectively characterize feature information of beautiful faces.
Investigation of Leader Motive Between Transformational Leadership and Pro-Social Voice: An Empirical Study in China

Prosocial voice, as a form of citizenship behavior with a purpose of expressing constructive changes and improving the status quo, is desirable in both teams and organizations. Also, transformational leadership (TL) has been documented as a leadership style that prompts employees to engage in prosocial voice. Recently, whether or not the effects of transformational leadership on prosocial voice have boundaries becomes a topic of interest to organizational researchers. We presented two separate models for the moderating effects of perceived leader motive (altruistic vs. instrumental) on the relationship between transformational leadership and prosocial voice in the workplace. From an employee perspective, this study documents that one’s perception towards his/her leader’s motive (altruistic vs. instrumental) underpinning leadership behaviors is related to the boundaries of TL’s effect on prosocial voice. Data with 167 employees at an auto maker in China were used and the analysis results provided support for the models.