Miracle-dz/UniFAR-FTUs huggingface dataset:
Retinal Thickness Asymmetry in Highly Myopic Eyes with Early Stage of Normal-Tension Glaucoma

Purpose To investigate the retinal thickness asymmetry parameters of circumpapillary retinal nerve fiber layer (cpRNFL) and macular layers measured by spectral-domain optical coherence tomography in highly myopic (HM) patients with an early stage of normal-tension glaucoma (NTG). Methods This cross-sectional study included 55 eyes of HM patients with early NTG and 37 eyes of HM normal participants. High myopia was defined as spherical equivalence more myopic than −6 diopters. Thickness differences and asymmetry indices (AIs) of cpRNFL between superior and inferior corresponding parts and thickness differences and AIs of the total macular layer (TML) and inner macular layers between superior and inferior hemispheres were calculated. The areas under the receiver operating characteristic curves (AROCs) were analyzed and compared. Results In the cpRNFL asymmetry analysis, the thickness differences and AIs of cpRNFL between temporal-superior and temporal-inferior sectors (P < 0.0001 and P < 0.0001, respectively) and between superior and inferior quadrants (P = 0.002 and P < 0.0001, respectively) were significantly different between HM control subjects and HM NTG patients. In the macular asymmetry analysis, the thickness difference and AI of TML were significantly different between superior and inferior hemispheres (P < 0.0001 and P < 0.0001, respectively). The thickness difference and AI of the macular ganglion cell layer (mGCL) were significantly different between superior and inferior hemispheres (P < 0.0001 and P < 0.0001, respectively). The AROCs for thickness difference of TML (0.845) and thickness difference of mGCL (0.773) were comparable to AROCs for average cpRNFL thickness (0.842), macular retinal nerve fiber layer thickness (mRNFL) thickness (0.871), and mGCL thickness (0.822). Conclusion In our study, HM NTG patients had retinal thickness asymmetry in cpRNFL, TML, and mGCL. The diagnostic capabilities for thickness asymmetry of TML and mGCL were comparable to the diagnostic capabilities for cpRNFL thickness, mRNFL thickness, and mGCL thickness. Asymmetry analysis of retinal thickness can be an adjunctive tool for the early detection of HM NTG.
Burnout and job dissatisfaction as negative psychological barriers in school settings: A mixed-methods investigation of Iranian teachers

BACKGROUND: Despite the multitude of studies comparing teacher burnout with an ample of variables, a need for seeking what English language teachers think about their job and the interfering variables regarding the context seems essential. The aim of this study was primarily to investigate the relationship between teacher burnout and job satisfaction of English language teachers and, then, to find out the impact of teaching experience and gender on teacher burnout and job satisfaction. MATERIALS AND METHODS: It was a mixed-methods study conducted on 103 teachers who were conveniently sampled and were investigated through questionnaires and interviews among high school English language teachers in Sistan and Baluchestan province. The sampling method was purposive, and data were collected through questionnaires and semi-structured interviews. Data analyses were performed using mixed–methods approach. RESULTS: The results revealed a moderate negative correlation between the first two elements of teacher burnout (emotional exhaustion and depersonalization) and job satisfaction (P < 0.01), whereas a positive correlation was found between the next element, i.e., personal accomplishments and job satisfaction (P < 0.05). Afterward, no statistically significant difference was detected between demographic characteristics (i.e., gender and teaching experience) (P < 0.01). Then, 15 teachers voluntarily participated in the interview sessions and expressed their opinions about the way teachers see the environment they work in. CONCLUSIONS: There are factors that influence on the quality of teaching and learning processes and lead to burnout and job dissatisfaction; therefore, it seems advisable to remove them to reduce their negative effects.
Tailoring Body Mass Index for Prediction of Obesity in Young Adults: A Multi-Centric Study on MBBS Students of Southeast India

Introduction: Body mass index (BMI) has been used for a long period as a surrogative measure for obesity. But BMI does not differentiate between fat and nonfat tissue (blood, bone, and muscle) due to which it is not considered accurate anymore. But since BMI is easier to estimate and used widely for assessment of obesity, it is better if it is re-standardized according to the body fat percentage (BFP) of a specific population, community, and their ethnicity. Objective: To estimate and propose the BMI cut-off values in young Indian population especially MBBS students taking BFP as a standard. Design: This is a cross-sectional study. Anthropometric data (age, gender, height, weight, waist circumference, and hip circumference) were collected from the participants after taking consent. BMI was calculated using Quetelet’s Rule. BFP was estimated using Omron Body fat Monitor (HBF 385). It measures the BFP by the bioelectrical impedance (BI) method. Data were analyzed with appropriate statistical tests and receiver operating curve (ROC) curves were drawn to find the cut-off values of BMI to determine obesity. Setting: The present study is a multi-centric study conducted in four medical colleges (two in each state; Odisha and Andhra Pradesh, India). Participants: Apparently healthy MBBS students aged 18-24 years were included in this study. Students having any chronic or acute illnesses were excluded from the study. Out of 904 students contacted from four medical colleges, 863 (430 males and 433 females) consented and participated. Results: Some 863 MBBS students have participated in this study. After adjusting for age, BMI was found to be higher in males. BMI was found to be 29.33 for males and in females it was 29.06. BFP was higher in females (34.23) as compared to males (20.77). Waist hip ratio was found to be higher in females (0.92) than in males (0.84). Whereas, fat free mass (FFM) and fat free mass index (FFMI) are higher in males, i.e., 56.24 and 18.48 respectively. Most appropriate cut-off value for obesity on ROC curve was found to be 22.09 (sensitivity 84.5%, specificity 83.46%) in males and that of females was 23.73 (sensitivity 85.26, specificity 81.23). Whereas, the conventional cut-off of 25 for males had sensitivity of only 46% and that of females was 70.5%. For total population BMI cut-off value was found to be 22.2 with 81% sensitivity and 74% specificity. Conclusion: We propose the cut-off value for overweight/obesity in males to be 22.09 kg/m2 and for females to be 23.73 kg/m2 in young adult Indian population. These values were found to have more sensitivity and specificity than current BMI cut-off value.
Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps

A random-pattern skin flap plays an important role in the field of wound repair; the mechanisms that influence the survival of random-pattern skin flaps have been extensively studied but little attention has been paid to endogenous counterinjury substances and mechanism. Previous reports reveal that the apelin-APJ axis is an endogenous counterinjury mechanism that has considerable function in protecting against infection, inflammation, oxidative stress, necrosis, and apoptosis in various organs. As an in vivo study, our study proved that the apelin/APJ axis protected the skin flap by alleviating vascular oxidative stress and the apelin/APJ axis works as an antioxidant stress factor dependent on CaMKK/AMPK/GSK3β signaling. In addition, the apelin/APJ-manipulated CaMKK/AMPK/GSK3β-dependent mechanism improves HUVECs' resistance to oxygen and glucose deprivation/reperfusion (OGD/R), reduces ROS production and accumulation, maintained the normal mitochondrial membrane potential, and suppresses oxidative stress in vitro. Besides, activation of the apelin/APJ axis promotes vascular migration and angiogenesis under relative hypoxia condition through CaMKK/AMPK/GSK3β signaling. In a word, we provide new evidence that the apelin/APJ axis is an effective antioxidant and can significantly improve the vitality of random flaps, so it has potential be a promising clinical treatment.
Role of Microneedling in Atrophic Post-Acne Scars: An Experience from a Tertiary Care Hospital

Objective To evaluate the outcomes of microneedling in patients with atrophic post-acne scars. Methodology A retrospective cross-sectional study was conducted at the Department of Dermatology, Patel Hospital for a duration of six months. Patients who were diagnosed with moderate to severe-grade atrophic acne scars were enrolled in the study. Patients with a history of photosensitivity, systemic lupus erythematosus, and xeroderma pigmentosum were excluded from the study. Goodman and Baron’s acne scar-grading system was used to evaluate the degree of atrophic scarring. A total of three sessions divided into four weekly intervals were conducted. The scar-grading was done before the procedure and after three treatment sessions using Goodman and Baron's acne scar grading system. Results A total of 50 patients were included in this study; 36 (72%) were females and 14 (28%) were males, with a mean age of 30.92±6.19 years. A decrease in the acne scar-grade was noted from grade III to grade II after three sessions of treatment. A significantly higher number of patients were in grade II after three-month sessions compared with before treatment (p=0.009), and a significantly lesser number of patients were in grade III (p=0.045). A significantly higher proportion of patients with moderate acne scars, as compared to severe, were in grade II (77.3% vs. 22.7%; p<0.001). Conclusion In this study, we found an improvement in the scar grade after microneedling sessions. However, as the study was retrospective, we suggest prospective randomized controlled trials in our set-up to better uncover the role of microneedling in reducing acne scars.
Fabrication and Characterization of the Core-Shell Structure of Poly(3-Hydroxybutyrate-4-Hydroxybutyrate) Nanofiber Scaffolds

Tissue engineering scaffolds with nanofibrous structures provide positive support for cell proliferation and differentiation in biomedical fields. These scaffolds are widely used for defective tissue repair and drug delivery. However, the degradation performance and mechanical properties of scaffolds are often unsatisfactory. Here, we successfully prepared a novel poly(3-hydroxybutyrate-4-hydroxybutyrate)/polypyrrole (P34HB-PPy) core-shell nanofiber structure scaffold with electrospinning and in situ surface polymerization technology. The obtained composite scaffold showed good mechanical properties, hydrophilicity, and thermal stability based on the universal material testing machine, contact angle measuring system, thermogravimetric analyzer, and other methods. The results of the in vitro bone marrow-derived mesenchymal stem cells (BMSCs) culture showed that the P34HB-PPy composite scaffold effectively mimicked the extracellular matrix (ECM) and exhibited good cell retention and proliferative capacity. More importantly, P34HB is a controllable degradable polyester material, and its degradation product 3-hydroxybutyric acid (3-HB) is an energy metabolite that can promote cell growth and proliferation. These results strongly support the application potential of P34HB-PPy composite scaffolds in biomedical fields, such as tissue engineering and soft tissue repair.
Improved sensitivity roll-off in dual reference, buffered spectral-domain optical coherence tomography

Abstract. Significance: While spectral-domain optical coherence tomography (SD-OCT) is a preferred form of OCT imaging, sensitivity roll-off limits its applicability for certain biomedical imaging applications. Aim: The aim of this work is to extend the imaging range of conventional SD-OCT systems for imaging large luminal organs such as the gastrointestinal tract. Approach: We present an SD-OCT system operating at a center wavelength of 1300 nm that uses two delayed reference arms to reduce sensitivity roll-off and an optical switch and a fiber optic delay line to ensure that the interference spectra are acquired from the same sample time window. Result: The proposed system was used to image swine colon ex vivo and duodenum in vivo, demonstrating improved image quality due to a ∼14 dB increase in sensitivity at the edges of the ranging depth. Conclusion: The proposed system requires modest hardware implementation and is compatible with catheter-based endoscopic helical scanning with enhanced sensitivity for the samples at a distance of ∼6 mm from the zero delay point.
Attitude and perception of gender equity among students and teachers of a rural school in West Bengal: A mixed-method approach

INTRODUCTION: “Gender” denotes the differences in the social roles of different sexes. This interaction results in variable health outcomes and care-seeking behavior. The present study was conducted to find out the attitude of school-going adolescents regarding gender equity and also to explore teachers’ perceptions in this context in a school of tribal belt of West Bengal. MATERIALS AND METHODS: A cross-sectional, mixed-method study was conducted among the students of classes eight to twelve, chosen by complete enumeration technique, with a predesigned, pretested validated questionnaire. The questionnaire was based on Gender Equitable Men scale and International Men and Gender Equality Survey questionnaire, comprising the following domains: ‘Gender restrictions’, ‘Gender attributes’, and ‘Gender domination’. The respondents were categorized into ‘high’, ‘moderate’, and ‘low’ groups as per their domain-wise attitude scores. In-depth interviews (IDIs) were conducted among seven teachers chosen purposively. Thematic analysis was done with the qualitative data. RESULTS: Out of 191 students, 112 were girls (58.6%) and 47 belonged to scheduled tribe category (24.6%). Overall, 170 (89.0%), 80 (41.9%), and 96 (50.3%) students had a high gender-equitable attitude regarding the domains of ‘gender restrictions’, ‘gender attributes’, and ‘gender domination’ respectively. Girls had higher percentage of high gender-equitable attitude in all the three domains (92.0%, 57.1%, and 50.9%, respectively). Regarding ‘gender restrictions’, 91.2% had high gender-equitable attitude in lower age group, compared to 81.4% in higher age group. Among the lower and higher age groups, 52.7% and 41.9% respectively, had high gender-equitable attitude on ‘gender domination’. Gender inequality amidst poverty, women deprived of health care, and gender violence were the major themes derived from the IDIs. CONCLUSION: Girls had a better attitude toward gender equity. Laws regarding gender dominance and violence should be focused. Conducting intensified awareness campaigns on gender equity and addressing women's right to health is a much-needed timely intervention for the health of womenfolk.
Characterizing reduced scattering coefficient of normal human skin across different anatomic locations and Fitzpatrick skin types using spatial frequency domain imaging

Abstract. Significance: Spatial frequency domain imaging (SFDI), a noncontact wide-field imaging technique using patterned illumination with multiple wavelengths, has been used to quantitatively measure structural and functional parameters of in vivo tissue. Using SFDI in a porcine model, we previously found that scattering changes in skin could potentially be used to noninvasively assess burn severity and monitor wound healing. Translating these findings to human subjects necessitates a better understanding of the variation in “baseline” human skin scattering properties across skin types and anatomical locations. Aim: Using SFDI, we aim to characterize the variation in the reduced scattering coefficient (μs′) for skin across a range of pigmentation and anatomic sites (including common burn locations) for normal human subjects. These measurements are expected to characterize baseline human skin properties to inform our use of SFDI for clinical burn severity and wound healing assessments. Approach: SFDI was used to measure μs′ in the visible- and near-infrared regime (471 to 851 nm) in 15 subjects at 10 anatomical locations. Subjects varied in age, gender, and Fitzpatrick skin type. Results: For all anatomical locations, the coefficient of variation in measured μs′ decreased with increasing wavelength. High intersubject variation in μs′ at visible wavelengths coincided with large values of the melanin extinction coefficient at those wavelengths. At 851 nm, where intersubject variation in μs′ was smallest for all anatomical locations and absorption from melanin is minimal, significant intrasubject differences in μs′ were observed at the different anatomical locations. Conclusions: Our study is the first report of wide-field mapping of human skin scattering properties across multiple skin types and anatomical locations using SFDI. Measured μs′ values varied notably between skin types at wavelengths where absorption from melanin was prominent. Additionally, μs′ varied considerably across different anatomical locations at 851 nm, where the confounding effects from melanin absorption are minimized.
Euryops arabicus Promotes Healing of Excised Wounds in Rat Skin: Emphasis on Its Collagen-Enhancing, Antioxidant, and Anti-Inflammatory Activities

Euryops arabicus Steud (E. arabicus) belongs to the family Asteraceae. It has several uses in folk medicine in the Arabian Peninsula. The current study aimed at evaluating the wound healing properties of the E. arabicus extract in rats. Primarily, E. arabicus successfully accelerated cell migration in vitro and it also showed no signs of dermal toxicity. Topical application of E. arabicus extract (5% or 20%) expedited healing of excised skin in rats. Histological examinations indicated that E. arabicus shortened epithelization period, stimulated fibroblast activity, and increased collagen deposition in wound tissues. The plant extract exerted antioxidant activity as evidenced by inhibition of GSH depletion and MDA accumulation and enhanced mRNA expression of Sod1 in wound tissues collected at the end of the experiment. Further, E. arabicus inhibited the rise of TNF-α and IL-1β in the skin wound region. The anti-inflammatory was confirmed by the observed down regulation of Ptgs2, Nos2, IL-6, and NF-κB mRNA expression. In addition, the extract enhanced the expression of TGF-β1 and HIF-1α in wounded skin tissues as indicated immunohistochemically. Conclusively, E. arabicus expedites excision wound healing in rats. Collagen-enhancing, anti-inflammatory, and antioxidant properties mediate the observed wound healing activity. These findings might contribute to our understanding of the ethnobotanical use of E. arabicus in wounds.
What Is the “Optimal” Target Mismatch Criteria for Acute Ischemic Stroke?

We aimed to compare Perfusion Imaging Mismatch (PIM) and Clinical Core Mismatch (CCM) criteria in ischemic stroke patients to identify the effect of these criteria on selected patient population characteristics and clinical outcomes. Patients from the INternational Stroke Perfusion Imaging REgistry (INSPIRE) who received reperfusion therapy, had pre-treatment multimodal CT, 24-h imaging, and 3 month outcomes were analyzed. Patients were divided into 3 cohorts: endovascular thrombectomy (EVT), intravenous thrombolysis alone with large vessel occlusion (IVT-LVO), and intravenous thrombolysis alone without LVO (IVT-nonLVO). Patients were classified using 6 separate mismatch criteria: PIM-using 3 different measures to define the perfusion deficit (Delay Time, Tmax, or Mean Transit Time); or CCM-mismatch between age-adjusted National Institutes of Health Stroke Scale and CT Perfusion core, defined as relative cerebral blood flow <30% within the perfusion deficit defined in three ways (as above). We assessed the eligibility rate for each mismatch criterion and its ability to identify patients likely to respond to treatment. There were 994 patients eligible for this study. PIM with delay time (PIM-DT) had the highest inclusion rate for both EVT (82.7%) and IVT-LVO (79.5%) cohorts. In PIM positive patients who received EVT, recanalization was strongly associated with achieving an excellent outcome at 90-days (e.g., PIM-DT: mRS 0-1, adjusted OR 4.27, P = 0.005), whereas there was no such association between reperfusion and an excellent outcome with any of the CCM criteria (all p > 0.05). Notably, in IVT-LVO cohort, 58.2% of the PIM-DT positive patients achieved an excellent outcome compared with 31.0% in non-mismatch patients following successful recanalization (P = 0.006). Conclusion: PIM-DT was the optimal mismatch criterion in large vessel occlusion patients, combining a high eligibility rate with better clinical response to reperfusion. No mismatch criterion was useful to identify patients who are most likely response to reperfusion in non-large vessel occlusion patients.
Purificação, caracterização físico-química e atividade anticoagulante de glicosaminoglicanos isolados da pele de tilápia do Nilo (Oreochromis niloticus)

Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). Glycosaminoglycans (GAGs) comprise the extracellular matrix of animal tissues, and residues from processing fish commercially harvested may offer new anticoagulant agents to substitute the heparin (HEP) in medical sciences. The aim of this study was to purify, characterize physico-chemically and evaluate the anticoagulant activity of GAGs isolated from the skin of Nile tilapia, Oreochromis niloticus. The GAGs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cystein and EDTA (5 mM), followed by ion-exchange chromatography on a DEAE-cellulose column using a NaCl gradient. The obtained fractions were lyophilizated and submitted to 0.5% agarose gel electrophoresis. The anticoagulant activity was assessed by the activated partial thromboplastin time (APTT) using normal human plasma and HEP standard (193 IU mg -1 ). The chromatographic profile separated into two different GAGs fractions (F I and F II) eluted at 0.50 and 0.75 M of NaCl, respectively, revealing fractions pattern distinct in the charge densities, but both showing GAGs with mobilities similar to standard dermatan sulfate (DS), suggesting the preliminary identification of this GAG. The GAGs modified the APTT, whose activities were, respectively, 4.72 (F I) and 23.80 (F II) IU mg -1 , and expressed themselves dose-dependent. Therefore, the anticoagulant DS from O. niloticus suggests an interesting source for posterior studies of antithrombotic activity.