Miracle-dz/UniFAR-FTUs huggingface dataset:
OTT_A_270688 11583..11594

1Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, People’s Republic of China; 2College of Translational Medicine, The First Affiliated Hospital of Jilin University, Jilin University, Changchun, Jilin 130021, People’s Republic of China; 3Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, People’s Republic of China Purpose: Ovarian cancer (OC) has the highest mortality among gynecological malignancies. Therefore, it is urgent to explore prognostic biomarkers to improve the survival of OC patients. One of the most prominent metabolic characteristics of cancer is effective glycolysis. Hexokinase 1 (HK1), as the first rate-limiting enzyme in glycolysis, is closely related to cancer progression. However, the role of HK1 in OC remains unclear. Materials and Methods: The Cancer Genome Atlas (TCGA) database was used to detect the expression of HK1 in OC patients. The chi-squared test was performed to examine the correlations between HK1 and patients’ clinical characteristics. Survival analyses were undertaken to determine the relationship between HK1 and patient survival, while the univariate/multivariate Cox model was used to evaluate the role of HK1 in patient prognosis. Gene Set Enrichment Analysis (GSEA) was performed to ascertain the related signaling pathways of HK1. RT-qPCR was implemented to validate the mRNA expression of HK1 in OC cells. MTT was used to detect cell viability after adding 2DG and knocking down HK1 in OC cells. HK1 protein expression was examined by Western blotting. Glucose uptake, lactate production, and ATP assays were undertaken following knockdown of HK1 in OC cells. Colony formation assays were performed to determine OC cell proliferation after HK1 knockdown. Transwell and wound healing assays were carried out to detect the invasion and migration of OC cells after HK1 knockdown. Results: We found that HK1 expression was increased in OC tissues and cells, and HK1 was related to the clinical characteristics of OC patients. Survival analysis revealed that OC patients in the HK1 overexpression group had poor survival. Moreover, univariant/multivariate analyses showed that HK1 may be an independent biomarker for the poor prognosis of OC patients. OC cell viability and proliferation decreased after knockdown of HK1. Consistently, glucose uptake, lactic acid production, ATP production, invasion, and migration were also decreased. Finally, GSEA enrichment analysis and Western blotting showed that HK1 was involved in MAPK/ERK signaling. Conclusion: HK1 may be a biomarker for the poor prognosis of OC patients and a potential therapeutic target.
Design and Simulation of a Dual-Mode Resonant Temperature Sensor based on MEMS Technology

: In this paper, a dual-mode (DM) micromechanical elliptical ring resonator for use in temperature sensing is reported. The proposed resonator is made of single crystal silicon and works based on beat frequency ( f b ). In the designed sensor, temperature coefficient of beat frequency (TC f b ) will be increased significantly by minimizing the f b and provides better temperature sensitivity. This proposed device was designed and simulated by COMSOL Multyphysics software. By engineering the device geometry, we introduce two adjacent resonant frequencies which produce very small f b in the range of 2 kHz. The device shows TC f 1 of about 44 ppm/ºC and TC f 2 of 5 ppm/ºC. Combination of small f b and large ΔTC f , present temperature coefficient of beat frequency (TC f b ) about 112000 ppm/ºC which has approximately 75 × improvement in TC f b compared to previous demonstrated DM resonators.
International Journal of Advanced Research in Computer Science and Software Engineering

: Computational grids that couple geographically distributed resources are becoming the de-facto computing platform for solving large-scale problems in science, engineering, and commerce. Software to enable grid computing has been primarily written for Unix-class operating systems, thus severely limiting the ability to effectively utilize the computing resources of the vast majority of Windows-based desktop computers. Addressing Windows-based grid computing is particularly important from the software industry‘s viewpoint where interest in grids is emerging rapidly. Microsoft‘s . NET Framework has become near-ubiquitous for implementing commercial distributed systems for Windows-based platforms, positioning it as the ideal platform for grid computing in this context. In this paper we present Alchemi1, a . NET based framework that provides the runtime machinery and programming environment required to construct enterprise/desktop grids and develop grid applications. It allows flexible application composition by supporting an object-oriented application programming model in addition to a file-based job model. Cross-platform support is provided via a web services interface and a flexible execution model supports dedicated and non-dedicated (voluntary) execution by grid nodes.
Facilitating Collaborative Engineering Analysis Problem Solving in Immersive Virtual Reality

Dr. Siddharth Savadatti received his PhD in Computational Mechanics from North Carolina State University in 2011 and has since been on the faculty of the College of Engineering at the University of Georgia. He teaches mechanics and numerical methods courses such as Statics, Fluid Mechanics, Programming, Numerical Methods for Engineers and Finite Element Analysis. In addition to traditional face-to-face classes, he has designed and taught courses in fully online and completely flipped formats.
DNA Sequence Analysis of rdxA and frxA from 12 Pairs of Metronidazole-Sensitive and -Resistant Clinical Helicobacter pylori Isolates

We previously reported that inactivation of rdxA and/or frxA converted Helicobacter pylori from metronidazole sensitive to metronidazole resistant. To examine the individual roles of rdxA and frxA in the development of metronidazole resistance in H. pylori , we examined the status of rdxA and frxA from 12 pairs of metronidazole-sensitive and -resistant H. pylori isolates obtained following unsuccessful therapy containing metronidazole. Arbitrary primed ﬁngerprinting analyses revealed that the genotypes of 11 sensitive and resistant pairs of strains were essentially identical. Amino acid sequence identities of RdxA and FrxA from the 14 metronidazole-sensitive isolates ranged from 92 to 98% and 95 to 98%, respectively, compared to that of H. pylori J99 (MIC, 1 m g/ml). All strains with high-level metronidazole resistance (MICs, 128 m g/ml) contained premature truncation of both RdxA and FrxA caused by nonsense and/or frameshift mutations. Strains with intermediate resistance to metronidazole (MICs, 32 to 64 m g/ml) contained a single premature truncation and/or altered RdxA and FrxA caused by nonsense, frameshift, and unique missense mutations. The low-level metronidazole- resistant strains (MICs, 8 m g/ml) contained unique missense mutations in FrxA but no speciﬁc changes in RdxA. The results demonstrate that alterations in both the rdxA and frxA genes are required for moderate and high-level metronidazole resistance and that metronidazole resistance that develops during anti- H. pylori therapy containing metronidazole is most likely to involve a single sensitive strain infection rather than a coinfection with a metronidazole-resistant strain. fragments were determined for both strands at the Molecular Genetics Core Facility of Baylor College of Medicine. DNA sequence determinations of the cloned fragments were repeated by SEQWRITE, Houston, Tex. , to conﬁrm the identity of the DNA sequences. In parallel, PCR ampliﬁcation and DNA se- quence determination of the known rdxA and frxA genes from H. pylori ATCC 700392 (26) were also performed to ensure PCR ampliﬁcation ﬁdelity. The resulting DNA sequences were analyzed by the Genetics Computer Group Programs (4), and the similarity search of the deduced protein sequences was performed by the BLASTX algorithm of the National Center for Biotechnology Information.
Physical Properties and Biodegradable Study of Metalized and Non-Metalized Polypropylene (PP) Films: A Comparative Research

Packaging industry plays vital role in the economy of Pakistan. Food packaging industries needs plastic films with well-defined barrier properties with respect to gases, humidity and aroma. The basic goal of this study was to assess the difference between barrier properties of metalized and non-metalized Polypropylene (PP) plastic films with respect of degradation. The PP films of 20 microns were produced by standard cast film extrusion process i.e. metalized PP films coated by aluminum and other without any coating. The main of this work is to compare the environmental impact of metalized and non-metalized CPP films. The samples were manufactured on industrial scale and to meet the food industry requirement metalized films has excellent barrier properties but it has taken about 30 days for small degradation (1 by 1cm metalized CPP). However the barrier properties of non-metalized CPP films shows poor result but it has been easily degraded as compare to metalize films. barrier properties of non-metalized with metallized PP films. Two types of Polypropylene (PP) films were manufactured by cast co-extrusion process on industrial scale. Barrier properties finished PP films were tested by using ASTMD-3985, D 882 and F1249standard methods. Results shows that non-metallized film has range of Water vapor transmission rate (WVTR) 6-8g/m 2 /day and Oxygen Transmission Rate (OTR) range 2300-2500cc/m²/atm/day. The obtained barrier properties data were considered from practical point of view. However metalized PP film shows about 50% to 80%improvement in the OTR and WVTR. It was estimated that metallization with aluminum has improved the barrier properties. Biodegradation of polymer is governing by nature of polymer and types of microorganism utilized it. In current studies the extend of degradation of plastic by fungal colonization (Aspergillus and Penicilliums) was measure by the structure investigation using FTIR spectroscopy, results indicate fewer changes in the peak after exposed to the fungi in synthetic media. The spectrum peak in the control on non-metalized film observe at 37736 cm -1 ,38913 cm -1 , and 6742 cm -1 , which are absent after degradation. Similarly, in metalized plastic peaks at 3779.8 cm -1 ,3771.1 cm -1 ,2357.2 cm -1 and 2007.2 cm -1 were disappeared after degradation. This indicates fungi use the plastic as the source of carbon and degrade it.
Analysis of One Dimensional Advection Diffusion Model Using Finite Difference Method

—In this paper, one dimensional advection diffusion model is analyzed using ﬁnite difference method based on Crank-Nicolson scheme. A practical problem of ﬁlter cake washing of chemical engineering is analyzed. The model is converted into dimensionless form. For the grid Ω × ω = [0 , 1] × [0 ,T ] , the Crank-Nicolson spatial derivative scheme is used in space domain and forward difference scheme is used in time domain. The scheme is found to be unconditionally convergent, stable, ﬁrst order accurate in time and second order accurate in space domain. For a test problem, numerical results are compared with the analytical ones for different values of parameter.
in Computer

- Cloud computing provides shared processing resources and data to computers and other devices on demand over the internet. Any configurable computing resource like, servers, networks, storage, applications and services can be provided immediately and released with lowest management effort. Although it seems highly useful and coherent, there is always the security and privacy concerns related to cloud as service providers can access the data in it at any time. Information could be altered or deleted or shared with third parties if required for the purpose of law and order without any prior notice.Authentication plays a majority role for information security that is a mechanism to find identity proof to get system access. In this paper we perform a survey of various security issues, existing user authentication techniques for cloud and discuss the growth as well as scope of multifactor authentication method in particular.
Effect of some Plant Extracts on Sugar Beet Powdery Mildew

his work was carried out under field conditions of two Governorates i.e. Menoufiya and Giza, during two successive sugar beet growing seasons (2013/14 and 2014/15). The efficacy of using extracts of four medicinal and aromatic plants, i.e. Fennel, Rosemary, Marjoram and Thyme extract by three organic solvents (ethanol, petroleum ether and chloroform) on the disease severity of sugar beet powdery mildew was studied. All treatments significantly reduced the disease severity of powdery mildew disease. Rosemary ethanol extract was the best treatment in reducing disease severity in the both seasons at the two governorate followed by fennal ethanol extract. On the other hand, marjoram petroleum ether extract was the least effective one. Also, the application of these treatments increased some crop parameters, i.e. total soluble solids (T.S.S), sucrose percent in root, purity of sugar and root weight.
DevelopmentReceptor Modulation During Thymocyte Receptor Knockin Mice Reveal Dynamic Green Fluorescent Protein-Glucocorticoid

To delineate the cellular targets and mechanisms by which glucocorticoids (GCs) exert their actions, we generated mice in which a green ﬂuorescent protein (GFP)-GC receptor (GR) fusion gene is knocked into the GR locus. In these mice, the GFP-GR protein, which is functionally indistinguishable from endogenous GR, allows the tracking and quantitation of GR expression in single living cells. In GFP-GR thymus, GR expression is uniform among embryonic thymocyte subpopulations but gradually matures over a 3-wk period after birth. In the adult, GR is speciﬁcally induced to high levels in CD25 (cid:1) CD4 (cid:2) CD8 (cid:2) thymocytes and returns to basal levels in CD4 (cid:1) CD8 (cid:1) thymocytes of wild-type and positively selecting female HY TCR-transgenic mice, but not negatively selecting male HY TCR-transgenic mice. In GFP-GR/recombinase-activating gene 2 (cid:2) / (cid:2) thymocytes, GR expression is down-regulated by pre-TCR complex stimulation. Additionally, relative GR expression is dissociated from GC-induced apoptosis in vivo. Results from these studies deﬁne differential GR expression throughout ontogeny, suggest pre-TCR activation as a speciﬁc mechanism of GR down-regulation, deﬁne immature CD8 (cid:1) thymocytes as novel apoptosis-sensitive GC targets, and separate receptor abundance from susceptibility to apoptosis across thymocyte populations. The Journal of Immunology, 2002, 169: 1309–1318.
A novel prediction model for diagnosing hepatocellular carcinoma in patients with chronic liver disease based on serological biomarkers

Background: Hepatocellular carcinoma (HCC), the most common type of digestive cancers, is a common complication of chronic liver disease (CLD). Currently, HCC is often diagnosed by radiological examination. 5-Hydroxymethylcytosine (5hmC) in a vital mammalian DNA epigenetic modification that is found in circulating cell-free DNA (cfDNA) and carries tumor-specific epigenetic information. It may also serve as a potential cancer biomarker for HCC diagnosis. Our study aimed to establish a statistical diagnostic model for diagnosing the risk of HCC in individual patients with CLD according objective tests, especially serological tumor markers. Methods:Based on the 5hmC-seal technique, the genome-wide-5-hydroxymethylcytosine (5hmC) and gene body 5hmC content in cfDNA samples from 96 patients were analyzed. Patients were recruited from the Second Affiliated Hospital of Nanchang University; 64 patients had HCC and 32 patients had CLD. Peripheral blood samples were prospectively collected, followed by blood preparation and cfDNA extraction. Independent risk factors associated with HCC were identified by logistic regression, and a diagnostic model was developed and evaluated based on sensitivity, specificity, and correct diagnostic results. Results:The ﬁnal model revealed sex, age, 5-Hydroxymethylcytosine (5hmC), and afetoprotein (AFP) as diagnostic indicators in a “discovery” data set. A novel statistical model was formed using the formula: Z = -18.160 + 0.081*age + 1.867*sex + 1.066*(logAFP) + 1.964*(log5hmC), which had an area under the receiver operating curve of 0.973. With 0.7636 as the cutoff point, the model had an estimated sensitivity of 0.891 and a specificity of 0.969. The novel model provides a reliable and stable capacity to predict HCC in patients with CLD. Conclusions: The study findings provide an objective blood serum marker-based model that could enhance the diagnostic efficiency of HCC and form a basis for the early detection of surgically resectable liver cancers in high-risk individuals.
Finite element solutions of the neutron transport equation with applications to strong heterogeneities

The Galerkin formulation of the finite element method is applied in space and angle to the equivalent integral law, or weak form, of the first-order neutron transport equation. The existence of a unique solution to the resultant system of algebraic equations is demonstrated using the positivity of the transport operator. Numerical results are given for the one-dimensional plane geometry application, including comparisons with the one-dimensional discrete ordinates code ANISN. A problem with strong heterogeneities is considered, and the use of discontinuous angular and spatial finite elements is shown to result in a marked improvement in the results. The success of the discontinuous elements is examined and it is seen that the discontinuous angular elements effectively match the analytical discontinuity in the angular flux at . mu. = 0 for plane geometry. Also, the use of discontinuous spatial elements is shown to result in treating continuity of the angular flux at an interface as a natural interface condition in the direction of neutron travel.
t-Dependent Pathway g Survival via a ROR T Cell Factor 1 Regulates Thymocyte

Survival of CD4 + CD8 + double-positive (DP) thymocytes plays a critical role in shaping the peripheral T cell repertoire. However, the mechanisms responsible for the regulation of DP thymocyte lifespan remain poorly understood. In this work, we demonstrate that T cell factor (TCF)-1 regulates DP thymocyte survival by upregulating ROR g t. Microarray analysis revealed that ROR g t was signiﬁcantly downregulated in TCF-1 2 / 2 thymocytes that underwent accelerated apoptosis, whereas ROR g t was greatly upregulated in thymocytes that had enhanced survival due to transgenic expression of a stabilized b -catenin ( b -cat Tg ), a TCF-1 activator. Both TCF-1 2 / 2 and ROR g t 2 / 2 DP thymocytes underwent similar accelerated apoptosis. Forced expression of ROR g t successfully rescued TCF-1 2 / 2 DP thymocytes from apoptosis, whereas ectopically expressed TCF-1 was not able to rescue the defective T cell development because of the lack of ROR g t-supported survival. Furthermore, activation of TCF-1 by stabilized b -catenin was able to enhance DP thymocyte survival only in the presence of ROR g t, indicating that ROR g t acts downstream of TCF-1 in the regulation of DP thymocyte survival. Moreover, b -catenin/TCF-1 directly interacted with the ROR g t promoter region and stimulated its activity. Therefore, our data demonstrated that TCF-1 enhances DP thymocyte survival through transcriptional upregulation of ROR g t, which we previously showed is an essential prosurvival molecule for DP thymocytes. The Journal of Immunology , 2011, 187: 5964–5973.
Rat Brain Microglia Prothrombin Kringle-2 Activates Cultured

Microglia, the major immune effector cells in the CNS, become activated when the brain suffers injury. In this study, we observed that prothrombin, a zymogen of thrombin, induced NO release and mRNA expression of inducible NO synthase, IL-1 (cid:1) , and TNF- (cid:2) in rat brain microglia. The effect of prothrombin was independent of the protease activity of thrombin since hirudin, a speciﬁc inhibitor of thrombin, did not inhibit prothrombin-induced NO release. Furthermore, factor Xa enhanced the effect of prothrombin on microglial NO release. Kringle-2, a domain of prothrombin distinct from thrombin, mimicked the effect of prothrombin in inducing NO release and mRNA expression of inducible NO synthase, IL-1 (cid:1) , and TNF- (cid:2) . Prothrombin and kringle-2 both triggered the same intracellular signaling pathways. They both activated mitogen-activated protein kinases and NF- (cid:3) B in a similar pattern. NO release stimulated by either was similarly reduced by inhibitors of the extracellular signal-regulated kinase pathway (PD98059), p38 (SB203580), NF- (cid:3) B ( N -acetylcysteine), protein kinase C (Go6976, bisindolylmaleimide, and Ro31-8220), and phospholipase C (D609 and U73122). These results suggest that prothrombin can activate microglia, and that, in addition to thrombin, kringle-2 is a domain of prothrombin independently capable of activating microglia. The Journal of Immunology,
Cyclic AMP-Independent Activation of Transcription Factor NF-KB in HL60 Cells by Tumor Necrosis Factors (x and 1B

No correlation exists in HL60 cells between NF-KcB activation by tumor necrosis factor (TNFa) and TNFI and intracellular levels of cyclic AMP. Cyclic AMP levels did not increase upon treatment of cells with each of these cytokines, although NF-KB was activated. Forskolin or 1-isobutyl-3-methylxanthine drastically increased intracellular levels of cyclic AMP, but neither activated NF-KB nor influenced TNF-induced NF-KB activation. Tumor necrosis factor a (TNFa) is a multifunctional cytokine which is released from macrophages upon treatment with several external stimuli (for a review, see reference 4). TNFa has diverse biological effects and induces expression of a number of different genes (see reference 15). TNFa activates transcription factor NF-KB (for reviews, see references 13, 14, and TNFa-induced of, e.g., the p55 the interleukin 2 (IL-2) and 31) and of the human immunodeficiency virus enhancer in chronically might, at in be mediated by NF-KB. NF-KB consists of two 51-kDa and two 65-kDa subunits. The 51-kDa subunits contain the DNA-binding the 65-kDa subunits for of NF-KB by and
Tissue Distribution of Cells Binding MRC 0 X22 Monoclonal Antibody Flow Cytofluorograph Analysis

Thymus-der ived cells of the mouse, rat, and human can be divided, on the basis of differential expression o f cell surface antigens, into phenotypically distinct subsets, and this phenotypic heterogenei ty is corre la ted with functional differences (1-3). In the rat, the monoclonal ant ibody W 3 / 2 5 (5) has been shown to label the T subset that contains helper cells for B cells (5) and cytotoxic T cells (6, 7) and also cells that mediate graft-versus-host (GvH) 1 reactivity (5). T h e monoclonal ant ibody MRC OX-8 labels the subset that contains suppressor and cytotoxic activities (2, 6, 8). In this paper a new monoclonal ant ibody MRC OX-22 is described that labels a subset of the W 3 / 2 5 ÷ cells in the rat. Data are presented demonstra t ing that this phenotypic division is corre la ted with a functional one, with cells mediating help for B cells and those mediat ing GvH reactivity in different T cell subsets. Biochemical studies of the MRC OX-22 antigen demonst ra te that it reacts with the high molecular weight form o f the rat leukocyte-common antigen (L-CA).