Microbiological Evaluation of Poultry Meat Obtained from Different Retail Markets in Khulna District

The research work conducted to evaluate microbial load in poultry meat from different retail markets in the Khulna district (Nirala, Dumuria, and Fultola). The objective of the present study was to get quality poultry products from commercial farms and retail markets. A total of 48 samples collected and brought to Quality Feed Lab. for laboratory assay. Samples were propagating in nutrient agar followed by culture on selective media, Eosin Methylene Blue Agar, Mac-Conkey agar, Brilliant Green Agar, Salmonella-Shigella Agar. Total numbers of 48 samples examined, and 30 samples were found positive to E. coli, and the prevalence of E. coli in this study was 62.20 %. 37.50% of thigh meat samples were positive for E. coli within 24 tested samples whereas 87.50% of breast meat samples were positive within 24 tested samples for this bacteria. On the other hand, 23 samples were found positive for Salmonella spp, and the prevalence of Salmonella spp in this study was 49.91 %. 29.16% of thigh samples were positive for Salmonella spp within 24 tested samples whereas 66.66% of litter samples were positive within 24 tested samples for this bacteria. Total Viable Count (TVC), Total Coliform Count (TCC), Total Salmonella Count (TSC), and Total Campylobacter Count (CPC) in meat samples of different broiler markets like Nirala market at Khulna city corporation, Dumuria, and Fultola were determined. GJMR-G Classification: NLMC Code: QW 70 MicrobiologicalEvaluationofPoultryMeatObtainedfromDifferentRetailMarketsinKhulnaDistrict Strictly as per the compliance and regulations of: Microbiological Evaluation of Poultry Meat Obtained from Different Retail Markets in Khulna District Bidyut Matubber , Joyanta Kumar Das , Md. Ahsan Habib , Sabuj Kanti Nath Ѡ & Md. Uzzal Hossain ¥ AbstractThe research work conducted to evaluate microbial load in poultry meat from different retail markets in the Khulna district (Nirala, Dumuria, and Fultola). The objective of the present study was to get quality poultry products from commercial farms and retail markets. A total of 48 samples collected and brought to Quality Feed Lab. for laboratory assay. Samples were propagating in nutrient agar followed by culture on selective media, Eosin Methylene Blue Agar, MacConkey agar, Brilliant Green Agar, Salmonella-Shigella Agar. Total numbers of 48 samples examined, and 30 samples were found positive to E. coli, and the prevalence of E. coli in this study was 62.20 %. 37.50% of thigh meat samples were positive for E. coli within 24 tested samples whereas 87.50% of breast meat samples were positive within 24 tested samples for this bacteria. On the other hand, 23 samples were found positive for Salmonella spp, and the prevalence of Salmonella spp in this study was 49.91 %. 29.16% of thigh samples were positive for Salmonella spp within 24 tested samples whereas 66.66% of litter samples were positive within 24 tested samples for this bacteria. Total Viable Count (TVC), Total Coliform Count (TCC), Total Salmonella Count (TSC) ,and Total Campylobacter Count (CPC) in meat samples of different broiler markets like Nirala market at Khulna city corporation, Dumuria, and Fultola were determined. Mean of TVC, TCC, TSC, and TCpC for the Nirala market at Khulna city corporation, Dumuria and Fultola markets were 5.61, 5.84, 6.29 log10 CFU/g, 4.72, 4.50, 4.47 log10 CFU/g, 3.19, 3.44, 3.49, log10 CFU/g and 2.31, 2.50, 2.54 log10 CFU/g, respectively. It observed that the mean values of TVC, TCC, TSC and TCpC in the case of Dumuria, and Fultola market exceeded the ICMSF recommendations, which may cause alarm to consumer’s health. The research work conducted to evaluate microbial load in poultry meat from different retail markets in the Khulna district (Nirala, Dumuria, and Fultola). The objective of the present study was to get quality poultry products from commercial farms and retail markets. A total of 48 samples collected and brought to Quality Feed Lab. for laboratory assay. Samples were propagating in nutrient agar followed by culture on selective media, Eosin Methylene Blue Agar, MacConkey agar, Brilliant Green Agar, Salmonella-Shigella Agar. Total numbers of 48 samples examined, and 30 samples were found positive to E. coli, and the prevalence of E. coli in this study was 62.20 %. 37.50% of thigh meat samples were positive for E. coli within 24 tested samples whereas 87.50% of breast meat samples were positive within 24 tested samples for this bacteria. On the other hand, 23 samples were found positive for Salmonella spp, and the prevalence of Salmonella spp in this study was 49.91 %. 29.16% of thigh samples were positive for Salmonella spp within 24 tested samples whereas 66.66% of litter samples were positive within 24 tested samples for this bacteria. Total Viable Count (TVC), Total Coliform Count (TCC), Total Salmonella Count (TSC) ,and Total Campylobacter Count (CPC) in meat samples of different broiler markets like Nirala market at Khulna city corporation, Dumuria, and Fultola were determined. Mean of TVC, TCC, TSC, and TCpC for the Nirala market at Khulna city corporation, Dumuria and Fultola markets were 5.61, 5.84, 6.29 log10 CFU/g, 4.72, 4.50, 4.47 log10 CFU/g, 3.19, 3.44, 3.49, log10 CFU/g and 2.31, 2.50, 2.54 log10 CFU/g, respectively. It observed that the mean values of TVC, TCC, TSC and TCpC in the case of Dumuria, and Fultola market exceeded the ICMSF recommendations, which may cause alarm to consumer’s health. Author α: Lecturer, Department of Microbiology and Public Health, Khulna Agricultural University, Khulna, Bangladesh. e-mail: bidyutm78@gmail.com Author σ: MS, Department of Microbiology and Public Health, Patuakhali Science and Technology University, Patuakhali, Bangladesh. e-mail: joydvm99@gmail.com Author ρ ¥: Lecturer, Department of Livestock Production and Management, Khulna Agricultural University, Khulna, Bangladesh. e-mail: ahhelal313@gmail.com Author Ѡ: Lecturer, Department of Animal Nutrition, Khulna Agricultural University, Khulna, Bangladesh.