Miracle-dz/UniFAR-FTUs huggingface dataset:
Modulation of Countermovement Jump-Derived Markers of Neuromuscular Function With Concurrent vs. Single-Mode Resistance Training.

Pattison, KJ, Drinkwater, EJ, Bishop, DJ, Stepto, NK, and Fyfe, JJ. Modulation of countermovement jump-derived markers of neuromuscular function with concurrent vs. single-mode resistance training. J Strength Cond Res XX(X): 000-000, 2020-This study assessed changes in countermovement jump (CMJ)-derived markers of neuromuscular function with concurrent training vs. resistance training (RT) alone and determined associations between changes in CMJ parameters and other neuromuscular adaptations (e.g., maximal strength gain). Twenty-three recreationally active men performed 8 weeks of RT alone (RT group, n = 8) or combined with either high-intensity interval training cycling (HIIT + RT group, n = 8) or moderate-intensity continuous cycling (MICT + RT group, n = 7). Maximal strength and CMJ performance were assessed before (PRE), after 4 weeks of training (MID), and >72 hours (maximal strength) or >5-7 days (CMJ performance) after (POST) the training intervention. Improvements in CMJ relative peak force from both PRE to MID and PRE to POST were attenuated for both HIIT + RT (effect size [ES]: -0.44; ±90% confidence limit, ±0.51 and ES: -0.72; ±0.61, respectively) and MICT + RT (ES: -0.74; ±0.49 and ES: -1.25; ±0.63, respectively). Compared with RT alone, the change in the flight time to contraction time ratio (FT:CT) was attenuated from PRE to MID for MICT + RT (ES: -0.38; ±0.42) and from PRE to POST for both MICT + RT (ES: -0.60; ±0.55) and HIIT + RT (ES: -0.75; ±0.30). PRE to POST changes in both CMJ relative peak force and flight time:contraction time (F:C) ratio were also associated with relative 1 repetition maximum leg press strength gain (r = 0.26 and 0.19, respectively). These findings highlight the utility of CMJ testing for monitoring interference to improvements in neuromuscular function with concurrent training.
A Comparison of Kinetic and Kinematic Variables During the Pull From the Knee and Hang Pull, Across Loads.

Meechan, D, McMahon, JJ, Suchomel, TJ, and Comfort, P. A comparison of kinetic and kinematic variables during the pull from the knee and hang pull, across loads. J Strength Cond Res XX(X): 000-000, 2020-Kinetic and kinematic variables during the pull from the knee (PFK) and hang pull (HP) were compared in this study. Eighteen men (age = 29.43 ± 3.95 years; height 1.77 ± 0.08 m; body mass 84.65 ± 18.79 kg) performed the PFK and HP with 40, 60, 80, 100, 120, and 140% of 1-repetition maximum (1RM) power clean, in a progressive manner. Peak force (PF), mean force (MF), peak system velocity (PSV), mean system velocity (MSV), peak power (PP), mean power (MP), and net impulse were calculated from force-time data during the propulsion phase. During the HP, small-to-moderate yet significantly greater MF was observed compared with the PFK, across all loads (p ≤ 0.001; Hedges g = 0.47-0.73). Hang pull PSV was moderately and significantly greater at 100-140% 1RM (p = 0.001; g = 0.64-0.94), whereas MSV was significantly greater and of a large-to-very large magnitude compared with PFK, across all loads (p < 0.001; g = 1.36-2.18). Hang pull exhibited small to moderate and significantly greater (p ≤ 0.011, g = 0.44-0.78) PP at 100-140%, with moderately and significantly greater (p ≤ 0.001, g = 0.64-0.98) MP across all loads, compared with the PFK. Hang pull resulted in a small to moderate and significantly greater net impulse between 100 and 140% 1RM (p = 0.001, g = 0.36-0.66), compared with PFK. The results of this study demonstrate that compared with the PFK, the HP may be a more beneficial exercise to enhance force-time characteristics, especially at loads of ≥1RM.
Comparison of mesenchymal stem cell sheets and chondrocyte sheets in a rabbit growth plate injury model

Background/aim The treatment of posttraumatic deformities and differences in length between the extremities resulting from physeal injury remains controversial. The aims of this study were to compare the efficacy of tissue-engineered, monolayer, and allogeneic mesenchymal stem cell sheets and chondrocyte sheets for physeal arrest treatment and to investigate cell sheet technology as a novel method for cell transplantation in physeal cartilage repair. Materials and methods A proximal tibial physeal injury was induced in New Zealand rabbits. Allogeneic mesenchymal stem cells (MSCs) and chondrocytes were cultured in temperature-responsive culture dishes and applied to the iatrogenic partial growth plate defects in single-sheet grafts (cell sheets). Treatment efficacy was determined using radiological measurements, as well as histological and immunohistochemical staining. Results Treatment with MSCs and chondrocytes prevented endochondral ossification in the physeal plate, and bone growth resumed after treatment in both the MSC and chondrocyte cell groups. We found significant differences in radiological evaluations between pre- and posttreatment measurements in both MSC and chondrocyte groups. Transplanted cells were observed in the damaged area in both of the groups, which differentiated in the direction of growth plate cartilage. Conclusion Our results support the hypothesis that MSC or chondrocyte transplantation using the cell-sheet technique described in the present study aids in the regeneration of cartilage tissue during physeal arrest after growth plate damage.
Dialogical Integration of Life Experiences and Successful Aging

This article examines the hypothesis that the dialogical integration of life experiences is related to successful aging. Life story interviews with 93 older Czech adults were sorted into categories characterized by specific patterns of life experience integration: (i) without dialogical processes, (ii) with differentiated I-positions, (iii) with dialogical relationships, (iv) partially integrated, and (v) completely integrated. The results indicated that the categories were ordered, yielding low-level correlations with scales of successful aging in predicted directions. A comparison of the categories revealed that they were related to successful aging in a cumulative way, starting with the most essential indicator (lower scores of rumination) in the participants who had developed at least dialogical relationships, continuing to higher well-being linked with partial integration, and ending with an advanced indicator (optimism toward future) linked with complete integration. These relationships were summarized in a hypothetical model that is open to further examination.
Accuracy of quantitative HIV‐1 RNA test methods at 1000 copies/mL and the potential impact of differences in assay calibration on therapy monitoring of patients

The World Health Organization (WHO) recommends the clinical use of a human immunodeficiency virus 1 (HIV‐1) viral load (VL) threshold level of 1000 copies (cp)/mL in patients on antiretroviral therapy (ART) to distinguish between viral control (VL < 1000 cp/mL) and viral failure or poor adherence (VL > 1000 cp/mL). The accuracy of five quantitative HIV‐1 RNA assays at this level was compared by replicate testing (n = 24) of 1000 cp/mL samples prepared from the Viral Quality Control (VQC) HIV‐1 subtype B standard, which is in use for validation of nucleic acid testing methods since 1995. Until 2004 the VL assays reported geometric mean (95% confidence interval [CI]) values ranging between 449 (188‐1067) and 3162 (3057‐2367) cp/mL when using the Siemens bDNA 3.0 assay as reference method for an assigned value of 1000 (962‐1038) cp/mL. In 2018, the following values (95% CI) were found by 24 replicate tests in each of the VL assays on the 1000 cp/mL samples: Abbott RealTime 1084 (784‐1572), BioMerieux EasyQ 1110 (533‐2230), Roche CAP/CTM 1277 (892‐1828), Hologic Aptima 1616 (1324‐1973), and Cepheid GeneXpert 2502 (1713‐3655) cp/mL. Calibration studies involving three consecutive WHO replacement standards showed a significant drift in the amount of RNA copies per International Unit overtime. Heat inactivation of HIV‐1 standards was found to cause a destandardizing effect. Our study underlines the limitations in HIV‐1 RNA assay calibration based on frequently replaced WHO international standards. It is therefore proposed that clinicians interpret the recommended 1000 cp/mL alert level in therapy monitoring with an inaccuracy range of 500 to 2000 cp/mL.
Cryo-EM structure in situ reveals a molecular switch that safeguards virus against genome loss

The portal protein is a key component of many double-stranded DNA viruses, governing capsid assembly and genome packaging. Twelve subunits of the portal protein define a tunnel, through which DNA is translocated into the capsid. It is unknown how the portal protein functions as a gatekeeper, preventing DNA slippage, whilst allowing its passage into the capsid, and how these processes are controlled. A cryo-EM structure of the portal protein of thermostable virus P23-45, determined in situ in its procapsid-bound state, indicates a mechanism that naturally safeguards the virus against genome loss. This occurs via an inversion of the conformation of the loops that define the constriction in the central tunnel, accompanied by a hydrophilic–hydrophobic switch. The structure also shows how translocation of DNA into the capsid could be modulated by a changing mode of protein–protein interactions between portal and capsid, across a symmetry-mismatched interface.
Yoga in school sports improves functioning of autonomic nervous system in young adults: A non-randomized controlled pilot study

Background Yoga in school is a beneficial tool to promote the good health and well-being of students by changing the way they react to stress. The positive effects of yoga—taught in schools—on children, youth and young adults have been demonstrated in former studies using mostly subjective psychometric data. Aim The present trial aims to evaluate the potential effects of yoga on autonomic regulation in young adults by analyzing heart rate variability (HRV). Methods This study is a non-randomized, explorative, two-arm-pilot study with an active control group. Fourteen healthy young adults took part in a 10-week yoga program (90 min once a week) in school and were compared to a control group of 11 students who participated in conventional school sports (90 min once a week over 10 weeks). 24-hour electrocardiograms (ECGs) were recorded at baseline and following the 10-week intervention. From 20-minute of nocturnal sleep phases, HRV parameters were calculated from linear (time and frequency domain) and nonlinear dynamics (such as symbolic dynamics and Poincaré plot analysis). Analyses of variance (ANOVA) followed by t-tests as post-hoc tests estimating both statistical significance and effect size were used to compare pre-post-intervention for the two groups. Results The statistical analysis of the interaction effects did not reveal a significant group and time interaction for the individual nocturnal HRV indices. Almost all indices revealed medium and large effects regarding the time main effects. The changes in the HRV indices following the intervention were more dramatic for the yoga group than for the control group which is reflected in predominantly higher significances and stronger effect sizes in the yoga group. Conclusion In this explorative pilot trial, an increase of HRV (more parasympathetic dominance and overall higher HRV) after ten weeks of yoga in school in comparison to regular school sports was demonstrated, showing an improved self-regulation of the autonomic nervous system.
Survival of Helicobacter pylori in drinking water and associated biofilms

Helicobacter pylori is a Gram-negative bacterium closely related to Campylobacter spp. Although most people who are infected with H. pylori are asymptomatic, the bacterium has been implicated in the development of certain diseases, such as gastric ulcers and stomach cancers. In the last 10 years, H. pylori has become one of the hot-topics for scientists who have managed to discover many aspects of the bacterium’s physiology, as well as describe its prevalence in the human population in several countries all around the world and clarify its role in certain human diseases. However, the route (or routes) of transmission among the human population are yet to be identified. This lack of information assumes particular importance to Portugal, a country where the data available indicates that H. pylori prevalence ranks amongst the highest in the European Community. With this work it was attempted to clarify the role of water as a means of transmission of H. pylori, with special emphasis being given to biofilms formed on pipe surfaces, where the diversity of microenvironments created by the heterotrophic consortium of bacteria could provide a more suitable environment for the survival of the pathogen. As such, a new molecular probe based on peptide nucleic acids was designed, which allow the in situ identification in this type of consortia. The application of this technique in lab-formed biofilms has shown that H. pylori subsists close to the basal layer of the biofilm, which suggests that the bacterium is able to migrate to low redox zones created by these structures to enhance survival. The influence of different environmental parameters on H. pylori adhesion was also studied. The bacteria appeared to adhere in higher numbers in the absence of shear forces, indicating biofilms formed on the surfaces exposed to well-water as preferential places for the location of the bacteria. After viability-staining of the adhered pathogen, it was observed that H. pylori would start to become non-viable after 48 hours, which indicates that co-aggregation with other species might be necessary for the survival of the bacteria. After being proved in this work that a nutrient shock occurred in H. pylori when it was recovered from water to a nutrient-rich media, a new low-nutrient medium was developed. The medium has caused an improvement of recovery of more than 30% for three out of
Influence of oocyte selection, activation with a zinc chelator and inhibition of histone deacetylases on cloned porcine embryo and chemically activated oocytes development

Summary The aim of this study was to evaluate the effects of alternative protocols to improve oocyte selection, embryo activation and genomic reprogramming on in vitro development of porcine embryos cloned by somatic cell nuclear transfer (SCNT). In Experiment 1, in vitro-matured oocytes were selected by exposure to a hyperosmotic sucrose solution prior to micromanipulation. In Experiment 2, an alternative chemical activation protocol using a zinc chelator as an adjuvant (ionomycin + N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) + N-6-dimethylaminopurine (6-DMAP)) was compared with a standard protocol (ionomycin + 6-DMAP) for the activation of porcine oocytes or SCNT embryos. In Experiment 3, presumptive cloned zygotes were incubated after chemical activation in a histone deacetylase inhibitor (Scriptaid) for 15 h, with the evaluation of embryo yield and total cell number in day 7 blastocysts. In Experiment 1, cleavage rates tended to be higher in sucrose-treated oocytes than controls (123/199, 61.8% vs. 119/222, 53.6%, respectively); however, blastocyst rates were similar between groups. In Experiment 2, cleavage rates were higher in zygotes treated with TPEN than controls but no difference in blastocyst rates between groups occurred. For Experiment 3, the exposure to Scriptaid did not improve embryo development after cloning. Nevertheless, the total number of cells was higher in cloned zygotes treated with Scriptaid than SCNT controls. In conclusion, oocyte selection by sucrose as well as treatments with zinc chelator and an inhibitor of histone deacetylases did not significantly improve blastocyst yield in cloned and parthenotes. However, the histone deacetylases inhibitor produced a significant improvement in the blastocyst quality.
Emerald Ash Borer (Agrilus planipennis) Density and Canopy Dieback in Three North American Ash Species

Emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae), a phloem-feeding insect native to Asia, was identified in 2002 as the cause of widespread ash (Fraxinus) mortality in southeast Michigan, U.S. and Windsor, Ontario, Canada. Little information about A. planipennis is available from its native range and it was not known whether this invasive pest would exhibit a preference for a particular North American ash species. We monitored A. planipennis density and canopy condition on green ash (F. pennsylvanica) and white ash (F. americana) street trees in four neighbor- hoods and on white and blue ash (F. quadrangulata) trees in two woodlots in southeast Michigan. Green ash street trees had significantly more canopy dieback and higher A. planipennis densities than white ash trees growing in the same neighborhood. Density increased by two- to fourfold in both species over a 3-year period. Canopy dieback increased linearly from 2002 to 2005 as A. planipennis density increased (R 2 0.70). In each of the woodlots, A. planipennis densities were significantly higher on white ash trees than blue ash trees. Woodpecker predation occurred in all sites and accounted for 35% of the A. planipennis that developed on trees we surveyed. Results indicate that surveys for A.
Substituted adamantylphthalimides: Synthesis, antiviral and antiproliferative activity

In this study, three groups of adamantylphthalimides, bearing different substituents at the phthalimide moiety, N‐(4′‐R2)phthalimidoadamantanes (1–7), 3‐[N‐(4′‐R2)phthalimido]‐1‐adamantanols (8–10), and 3‐[N‐(4′‐R2)phthalimido]adamantane‐1‐carboxylic acids (11–15), were synthesized and screened against tumor cells and viruses. The most potent compounds are not substituted at the adamantane and bear an OH or NH2 substituent at the phthalimide (compounds 3 and 5). The antiproliferative activities of compounds 3 and 5 are in the micromolar range, much higher than the one of thalidomide. A minor antiviral activity against cytomegalovirus and varicella‐zoster virus was found for compounds 3 and 5, but these compounds lacked selectivity. The results presented are important for the rational design of the next‐generation compounds with anticancer and antiviral activities.
FAK Promotes Early Osteoprogenitor Cell Proliferation by Enhancing mTORC1 Signaling

Focal adhesion kinase (FAK) has important functions in bone homeostasis but its role in early osteoprogenitor cells is unknown. We show herein that mice lacking FAK in Dermo1‐expressing cells exhibited low bone mass and decreased osteoblast number. Mechanistically, FAK‐deficient early osteoprogenitor cells had decreased proliferation and significantly reduced mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling, a central regulator of cell growth and proliferation. Furthermore, our data showed that the pharmacological inhibition of FAK kinase‐dependent function alone was sufficient to decrease the proliferation and compromise the mineralization of early osteoprogenitor cells. In contrast to the Fak deletion in early osteoprogenitor cells, FAK loss in Col3.6 Cre‐targeted osteoblasts did not cause bone loss, and Fak deletion in osteoblasts did not affect proliferation, differentiation, and mTORC1 signaling but increased the level of active proline‐rich tyrosine kinase 2 (PYK2), which belongs to the same non–receptor tyrosine kinase family as FAK. Importantly, mTORC1 signaling in bone marrow stromal cells (BMSCs) was reduced if FAK kinase was inhibited at the early osteogenic differentiation stage. In contrast, mTORC1 signaling in BMSCs was not affected if FAK kinase was inhibited at a later osteogenic differentiation stage, in which, however, the concomitant inhibition of both FAK kinase and PYK2 kinase reduced mTORC1 signaling. In summary, our data suggest that FAK promotes early osteoprogenitor cell proliferation by enhancing mTORC1 signaling via its kinase‐dependent function and the loss of FAK in osteoblasts can be compensated by the upregulated active PYK2. © 2020 American Society for Bone and Mineral Research.
Usual care and a self-management support programme versus usual care and a relaxation programme for people living with chronic headache disorders: a randomised controlled trial protocol (CHESS)

Introduction Chronic headaches are poorly diagnosed and managed and can be exacerbated by medication overuse. There is insufficient evidence on the non-pharmacological approaches to helping people living with chronic headaches. Methods and analysis Chronic Headache Education and Self-management Study is a pragmatic randomised controlled trial to test the effectiveness and cost-effectiveness of a self-management education support programme on top of usual care for patients with chronic headaches against a control of usual care and relaxation. The intervention is a 2-day group course based on education, personal reflection and a cognitive behavioural approach, plus a nurse-led one-to-one consultation and follow-up over 8 weeks. We aim to recruit 689 participants (356 to the intervention arm and 333 to the control) from primary care and self-referral in London and the Midlands. The trial is powered to show a difference of 2.0 points on the Headache Impact Test, a patient-reported outcome measure at 12 months post randomisation. Secondary outcomes include health related quality of life, self-efficacy, social activation and engagement, anxiety and depression and healthcare utilisation. Outcomes are being measured at 4, 8 and 12 months. Cost-effectiveness will be expressed in terms of incremental cost per quality-adjusted life year gained. Ethics and dissemination This trial will provide data on effectiveness and cost-effectiveness of a self-management support programme for chronic headaches. The results will inform commissioning of services and clinical practice. North West – Greater Manchester East Research Ethics Committee have approved the trial. The current protocol version is 3.6 date 7 March 2019. Trial registration number ISRCTN79708100.
Chloroquine and bafilomycin A mimic lysosomal storage disorders and impair mTORC1 signalling

Abstract Autophagy is dependent upon lysosomes, which fuse with the autophagosome to complete the autophagic process and whose acidic interior permits the activity of their intraluminal degradative enzymes. Chloroquine (CQ) and bafilomycin A1 (BafA) each cause alkalinisation of the lumen and thus impair lysosomal function, although by distinct mechanisms. CQ diffuses into lysosomes and undergoes protonation, while BafA inhibits the ability of the vacuolar type H+-ATPase (v-ATPase) to transfer protons into the lysosome. In the present study, we examine the impact of CQ and BafA on the activity of mammalian target of rapamycin complex 1 (mTORC1), inhibition of which is an early step in promoting autophagy. We find each compound inhibits mTORC1 signalling, without affecting levels of protein components of the mTORC1 signalling pathway. Furthermore, these effects are not related to these agents’ capacity to inhibit autophagy or the reduction in amino acid supply from lysosomal proteolysis. Instead, our data indicate that the reduction in mTORC1 signalling appears to be due to the accumulation of lysosomal storage material. However, there are differences in responses to these agents, for instance, in their abilities to up-regulate direct targets of transcription factor EB (TFEB), a substrate of mTORC1 that drives transcription of many lysosomal and autophagy-related genes. Nonetheless, our data imply that widely used agents that alkalinise intralysosomal pH are mimetics of acute lysosomal storage disorders (LSDs) and emphasise the importance of considering the result of CQ and BafA on mTORC1 signalling when interpreting the effects of these agents on cellular physiology.