Miracle-dz/UniFAR-FTUs huggingface dataset:
Cloning of AsRBP1 Gene from Oat(Avena sterilis) and Analysis on Its Expressions to Stresses

Glycine-rich RNA-binding proteins(GR-RBPs) play important roles in the regulation of stress and improve the resistance in plants.In this study,in order to mine stress-related genes and supply important candidate genes for transgenic wheat,AsRBP1 was cloned from Avena sterilis based on homologous cloning method.The results showed that the cDNA of AsRBP1 was 447 bp in length and encoded 148 amino acids.There was a typical RNA recognition motif(RRM) in the N-terminal in AsRBP1 and Glycine-rich domain in the C-terminal,which content was 35.8 %. The Phylogenetic tree showed that AsRBP1 and Arabidopsis AtGR-RBP7 had a close relationship.Real-time PCR analysis of the expression characteristics showed that AsRBP1 was significantly induced by exogenous abscisic acid and low temperature,and at the same time it responded to drought and high salt stress.Therefore,it was speculated that AsRBP1 was a member of GR-RBPs family and might be involved in stress response and enhanced the stress resistance of plants.Finally,it was very useful for wheat molecular breeding aspect to provide good candidate stress genes.
Polymorphic short tandem repeats for diagnosis of the Charcot-Marie-Tooth 1A duplication

Objective:Short tandem repeats (STRs) sequences 4A, 9A and 9B were used to detect gene dup lication of Charcot Marie Tooth disease type 1A(CMT1A). Methods: Polymorphism and heterozygosity of STR 4A, 9A and 9B were analyzed in 100 nor mal controls using polymerase chain reaction and Single strand conformational p olymophisms (PCR SSCP). Then STR 4A, 9A and 9B were used to detect gene duplica tion in 30 unrelated CMT1 patients. Results: The polymorphi sm of the 3 markers is 8, 12 and 11 respectively. The heterozygosity of them is 0 75, 0 84 and 0 81 respectively. 60%(18/30) of CMT1 patients were detected gene duplication using 3 markers. The rate is higher than that(52 9%) which was detected by the primary markers in our laboratory. Conclusion:B oth polymorphism and heterozygosity of STR 4A, 9A and 9B are high in normal cont rols and in CMT1 patients. Especially 9A and 9B have the highest observed hetero zygosity reported to date for CMT1A markers. They are the ideal markers to detec t CMT1A gene duplication clinically.
Rainfall simulators and USDA erosion research: history, perspective, and future

Erosion problems have perplexed mankind throughout recorded history. Activities which generally accelerate erosion above that known as geologic erosion have been responsible for migrations of major cultures from one area to another. This accelerated erosion has been ofmajor concern toconservationists inthe20th Century asintensive farming hasdepleted the soil resource. The earliest quantitative erosion research measurements made in the U.S. were begun by Sampson and associates in 1912 on overgrazed rangelands in Central Utah. Unfortunately, after these efforts, erosion measurements and research to control erosion on rangelands languished until the latter part of the 1970's. Thus, current technology for controlling erosion onrangelands was de veloped primarily for cultivated croplands and transferred, with minimal validation, to rangelands. Rainfall simulators have played, and continue to play, animpor tant role in erosion research because they facilitate controlled experiments at known antecedent conditions which permit orderly evaluation of oneor more factors known to affect erosion pro cesses. The advantages and disadvantages of some of the more commonly used simulators used by the Agricultural Research Service are discussed.
Planting Time Affects Vegetative and Reproductive Growth of Exotic Cultivars of Snapdragon (Antirrhinum majus)

The field performance of 24 exotic cultivars of snapdragon (Antirrhinum majus L.) at three planting dates (November 10, 2009; March 10 and October 10, 2010) was assessed. Data of vegetative traits i.e., number of leaves, nodes, secondary shoots, plant height, fresh weight per plant and reproductive traits, including days to bud opening, spike length, and number of buds/spike, were recorded. Six cultivars (‘Potomac Early Rose’, ‘Maryland Lavender’, ‘Potomac Royal’, ‘Potomac White’, ‘Potomac Early Orange’ and ‘Monaco Violet’) showed superior vegetative growth while five cultivars (‘Potomac Early Pink’, ‘Maryland Golden Brnz’, ‘Monaco Yellow’, ‘Potomac Orange Dark’ and ‘Monaco Baltimore Rose’) performed poorly. ‘Potomac Early Rose’ and ‘Maryland Lavender’ performed best reproductively, and produced better quality flower spikes than all other cultivars. When considering the reproductive performance of these 24 snapdragon cultivars, sowing on November 10, 2009 was most suitable compared to the two other sowing dates. © 2016 Friends Science Publishers
Identification of the Barley stripe mosaic virus (BSMV) from Beijing

BSMV was detected in the infected barley leaves collected from Beijing by ELISA with the antiserum of BSMV Xinjing Isolate(BSMV-XJ). The virus appeared to be rod-shaped,detected by electron microcopy.In indoor inoculation test,this virus proved to be able to infect barley,wheat,Nicotiana benthamlana and Chenopodium Amaranticolor and could be transmitted by barley seeds.The coat protein of BSMV was amplified from the total RNA of infected barley leaves by RT-PCR for the first time in China.All the results above indicate that the virus is one isolate of BSMV,which may be a new isolate in China for the difference from BSMV-XJ in its host range and seed transmission.
Comparison of four microbial communities with wheat straw-degradation capability at normal temperature

Four microbial communities named WSD-5,N,M and D,which with stable wheat straw-degradation capability,were constructed at normal temperature using the restricted cultivation technology.Compared with other three groups,WSD-5 exhibited the potential higher wheat straw-degradation ability during the process of continuous cultivation.On the 15th day,WSD-5 displayed the maximum wheat straw-degradation rate up to 75.6%,compared to N(65.0%),M(69.4%) and D(67.5%). Based on the results,up to 94.2% of cellulose,81.9% of hemicellulose,and 21.3% of lignin could be decomposed by WSD-5.The activities of cellulase,xylanase,and filter paper were detected for all the four communities and the results demonstrated that WSD-5 showed the highest enzyme activity(filter paper activity,1.30 U/mL;cellulose endonuclease activity,4.35 U/mL;exonuclease,0.60 U/mL;β-glucosidase activity,0.43 U/mL;xylanase activity,15.16 U/mL). The result of specific primer PCR showed that WSD-5 was consisted of fungi and bacteria together.
Far‐red dependent changes in the chemical composition of Spirulina platensis

The influence of far‐red light (FRL) was studied on the chemical composition of Spirulina platensis biomass. The following light compositions were used during the culture white light, blue‐red LED light (BRL) and BRL supplemented with FRL (BRFRL). Chlorophyll and phenol contents were measured by spectrophotometric methods, whereas presence of carotenoids, lipids, and phycobiliproteins were estimated by Fourier‐transform Raman spectrometry. Additionally, phenol content was investigated by fluorescence intensity of algae culture in the range of 430–650 nm. The content of chlorophyll and phenols in algae cells depended on the spectral composition of light and was the highest under BRL (16.7 ± 0.5 and 9.1 ± 0.6, respectively). It was shown that there is a positive linear correlation (R = 0.902 at p < 0.0000001) between the ratio of relative fluorescence intensity of S. platensis suspensions at 450 nm to the suspensions at 540 nm (F450/F540) and the content of phenolic compounds in the biomass. Changes in the F450/F540 ratio can explain approximately 80% changes of phenol contents in algae cells. Spirulina platensis Raman spectra demonstrated that the biomass of algae growing under white light and BRL had a significantly higher intensity of phycobiliprotein bands than the algae growing under BRFRL.
Comparison of Methods about Paeonia lutea's Pollen Viability Determination

The fresh pollen of Paeonia lutea was employed as experimental material to study the pollen viability.The effects of different concentration of sucrose,boron,calcium,magnesium and potassium on the germination of P.lutea's pollen were compared through simple factorial experiment.Based on these,the orthogonal design was used to compare the effects of sucrose,boron and calcium on the germination of P.lutea's pollen.Carmine acetate staining,I2-KI staining,and TTC staining were compared in order to seek for a fast determination method of P.lutea's pollen viability.The results showed that the sucrose and boron had great effects on the germination of P.lutea's pollen.Under the optimum pH 6.0 values,the optimum culture solution was sucrose 150 g·L-1 + H3BO3 30 mg·L-1 + CaCl2 20 mg·L-1,the germination rate was 68.7%. When cultured in pure water,the protoplasm of the pollen wouldn't split,and the substance inside wouldn't flow out,but its germination rate was very low.When cultured in the solution with high concentration of sucrose or salt,the protoplasm would dehydrate and separate from the cell wall.These two would inhibit the germination of P.lutea's pollen.And TTC staining showed that the viability rate was 64.9% which was the optimum staining for the fast determination of P.lutea's pollen viability.
Perspectives on contemporary Welsh language maintenance

Analyse des donnees du dernier recensement linguistique au Pays de Galles (1981), comparaison avec les donnees du precedent recensement (1971), et perspectives sur le maintien de la langue. Stabilite des differentes zones geographiques (foyers de resistance — zones ceintures — zones anglicisees). Si l'on enregistre en general un declin de 2 a. 4 %, les regions industrialisees du sud-est, du nord-est et le Radnor connaissent une remontee des galloisants : cela est du a la promotion de l'enseignement du gallois dans ces regions. La langue est donc plus presente dans les grandes villes — presence precaire dans des milieux ou l’anglais domine de facon ecrasante.
Chronic Urticaria Associated with Helicobacter pylori

A 31-year-old male presented with a complaint of chronic pruritus with diffuse urticarial wheals for the past seven months. He underwent extensive workup and was found to have positive Helicobacter pylori (H. pylori) stool antigen test. He was treated with bismuth subsalicylate, metronidazole, tetracycline, and omeprazole for two weeks. Pruritus and urticarial wheals disappeared in four weeks after therapy was started.
Osmoregulation of gene expression in Salmonella typhimurium: proU encodes an osmotically induced betaine transport system

Previous evidence has indicated that a gene, proU, is involved in the response of bacterial cells to growth at high osmolarity. Using Mu-mediated lacZ operon fusions we found that transcription of the proU gene of Salmonella typhimurium is stimulated over 100-fold in response to increases in external osmolarity. Our evidence suggests that changes in turgor pressure are responsible for these alterations in gene expression. Expression of proU is independent of the ompR gene, known to be involved in osmoregulation of porin expression. Thus, there must be at least two distinct mechanisms by which external osmolarity can influence gene expression. We show that there are relatively few genes in the cell which are under such osmotic control. The proU gene is shown to encode a high-affinity transport system (Km = 1.3 microM) for the osmoprotectant betaine, which is accumulated to high concentrations in response to osmotic stress. Even when fully induced, this transport system is only able to function in medium of high osmolarity. Thus, betaine transport is regulated by osmotic pressure at two levels: the induction of expression and by modulation of activity of the transport proteins. We have previously shown that the proP gene encodes a lower-affinity betaine transport system (J. Cairney, I. R. Booth, and C. F. Higgins, J. Bacteriol., 164:1218-1223, 1985). In proP proU strains, no saturable betaine uptake could be detected although there was a low-level nonsaturable component at high substrate concentrations. Thus, S. typhimurium has two genetically distinct pathways for betaine uptake, a constitutive low-affinity system (proP) and an osmotically induced high-affinity system (proU).
Perspectives on the genomics research of important crops in the tribe Andropogoneae: Focusing on the Saccharum complex

Climate changes are shifting the perception of C4 photosynthetic crops due to their superior adaptability to harsh conditions. The tribe Andropogoneae includes some economically important grasses, such as Zea mays, Sorghum bicolor, Miscanthus spp. , and Saccharum spp. , representing C4 photosynthetic grasses. Although the Andropogoneae grasses diverged fairly recently, their genomic structures are remarkably different from each other. As previously reported, the family Poaceae shares the pan-cereal duplication event occurring ca. 65 MYA. Since this event, Sorghum bicolor has never experienced any additional duplication event. However, some lineage-specific duplication events were reported in Z. mays and Saccharum spp. , and, more recently, it was revealed that a shared allotetraploidization event occurred before the divergence between Miscanthus and Saccharum (but after the divergence from S. bicolor), which provided important clues to those two species having large genome sizes with complicated ploidy numbers. The complex genomic structures of sugarcane and Miscanthus (defined as the Saccharum complex along with some other taxa) have had a limiting effect on the use of their molecular information in breeding programs. For the last decade, genomics-associated technologies have become an important tool for molecular crop breeding (genomics-assisted breeding, GAB), but it has not been directly applied to sugarcane and Miscanthus due to their complicated genome structures. As genomics research advances, molecular breeding of those crops can take advantage of technical improvements at a reasonable cost through comparative genomic approaches. Active genomic research of non-model species using closely related model species will facilitate the improvement of those crops in the future.
The association between c.553G>T polymorphism in apolipoprotein A5 gene and level of serum lipid

Objective To investigate the association between c.553GT polymorphism in apo A5 gene and the level of serum lipid in the Han population of Jiangsu Province,China.Methods Using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)we analyzed the genotypes and calculated the frequency of alleles in 199 hypertriglyceridemia(HTG)patients and 225 healthy individuals.The levels of serum lipid profiles were also studied by biochemical methods.Results The frequency of T alleles of c.553GT polymorphism in HTG patients was significantly higher than that of the control group(12.56% vs 4.67%,P0.001). Compared with the wild type GG,TT homozygotes and TG heterozygotes also showed a correlation with increased serum concentration of TG (P0.001) and decreased concentration of HDL-C (P=0.011). Conclusions The apoA5 c.553GT polymorphism is associated with the elevation of serum triglyceride,and is also strongly related to hypertriglyceridemia.
recA is required in the induction of pectin lyase and carotovoricin in Erwinia carotovora subsp. carotovora

Pectin lyase (PNL) and the bacteriocin carotovoricin (CTV) were induced in Erwinia carotovora subsp. carotovora 71 by the DNA-damaging agents mitomycin C, nalidixic acid, and UV light. To determine whether the recA product was involved in the expression of these damage-inducible phenotypes, we cloned the E. carotovora subsp. carotovora recA+ gene, inactivated it by Tn5 insertion, and constructed an E. carotovora subsp. carotovora recA::Tn5 strain by gene replacement via homologous recombination. The RecA- strain was more sensitive to methyl methanesulfonate, nitroquinoline oxide, and UV light than its RecA+ parent. The recA mutation did not affect the production of pectate lyase, polygalacturonase, cellulase, and protease or the ability to cause soft rot of potato tubers. With this mutant, unlike with the RecA+ parent strain, PNL and CTV were not induced by mitomycin C or detected in potato tuber tissue. The RecA+ phenotype, including the inducibility of PNL and CTV, could, however, be restored in the mutant in trans by the recA+ gene from either E. carotovora subsp. carotovora or Escherichia coli. We conclude that, in E. carotovora subsp. carotovora, the recA product is required in the induction of PNL and CTV.
New Bromoperoxidase from Coralline Algae that Brominates Phenol Compounds

Bromoperoxidases were found in coralline algae (Corallinaceae, Rhodophyta) collected from seasides in Japan, and high enzyme activities were observed in Corallina officinalis, Corallina pilulifera and Amphiroa zonata. The optimum pHs of the enzymes from coralline algae were around 6.0. The enzymes were specific for I− and Br−, and did not act on Cl− and F−. The enzymes purified from Corallina pilulifera and Amphiroa ephedraea catalyzed the brominations of phenol and O-hydroxybenzyl alcohol in the presence of Br− and H202 to form the same product, 2,4,6-tribromophenol.
Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis

We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Chromosomal DNA from dinger zones (visible subsurface zones of leptospiral growth) of first semisolid subcultures of field isolates was also isolated and characterized, thus greatly speeding up the diagnostic process.
Review of Steady-State Electric Power Distribution System Datasets

Publicly available grid datasets with electric steady-state equivalent circuit models are crucial for the development and comparison of a variety of power system simulation tools and algorithms. Such algorithms are essential to analyze and improve the integration of distributed energy resources (DERs) in electrical power systems. Increased penetration of DERs, new technologies, and changing regulatory frameworks require the continuous development of the grid infrastructure. As a result, the number and versatility of grid datasets, which are required in power system research, increases. Furthermore, the used grids are created by different methods and intentions. This paper gives orientation within these developments: First, a concise overview of well-known, publicly available grid datasets is provided. Second, background information on the compilation of the grid datasets, including different methods, intentions and data origins, is reviewed and characterized. Third, common terms to describe electric steady-state distribution grids, such as representative grid or benchmark grid, are assembled and reviewed. Recommendations for the use of these grid terms are made.
A comparison of deep machine learning algorithms in COVID-19 disease diagnosis

The aim of the work is to use deep neural network models for solving the problem of image recognition. These days, every human being is threatened by a harmful coronavirus disease, also called COVID-19 disease. The spread of coronavirus affects the economy of many countries in the world. To find COVID-19 patients early is very essential to avoid the spread and harm to society. Pathological tests and Chromatography(CT) scans are helpful for the diagnosis of COVID-19. However, these tests are having drawbacks such as a large number of false positives, and cost of these tests are so expensive. Hence, it requires finding an easy, accurate, and less expensive way for the detection of the harmful COVID-19 disease. Chest-x-ray can be useful for the detection of this disease. Therefore, in this work chest, x-ray images are used for the diagnosis of suspected COVID-19 patients using modern machine learning techniques. The analysis of the results is carried out and conclusions are made about the effectiveness of deep machine learning algorithms in image recognition problems.
Leukocyte esterase-nitrite and bioluminescence assays as urine screens

The 1-min leukocyte esterase (LE)-nitrite test (Chemstrip 9; Biodynamics, Division of Boehringer Mannheim Biochemicals, Indianapolis, Ind.) and a bioluminescence assay (Monolight centrifugation method; Analytical Luminescence Laboratory, Inc., San Diego, Calif.) were tested for their efficacy as urine screens among 453 patients at a tertiary-care teaching hospital. Both methods had the capacity to exclude significant bacteriuria (greater than or equal to 10(5) CFU/ml) when compared with the results of conventional culture methods, with predictive values of 99 and 93%, respectively, for a negative test. Bioluminescence was the more accurate nonculture method used. Sensitivity and specificity values were 97 and 71%, respectively, for bioluminescence, 82 and 60%, respectively, for LE with nitrite, and 72 and 64%, respectively, for LE without nitrite. At reduced levels of bacteriuria less than 10(5) CFU/ml), the sensitivities of LE-nitrite and bioluminescence were decreased but comparable. The addition of protein and blood test results in the Chemstrip 9, along with LE-nitrite as bacteriuria indicators, were unsatisfactory because of the large numbers of false-positive results attributed to protein and blood determinations. LE activity as detected by the LE test was a poor predictor of significant bacteriuria in both male and female patients. The sensitivity (71%) and specificity (57%) of the LE test in male patients were significantly lower than those previously reported and varied with the patient population studied.
Universal primer PCR with SSCP and RFLP for identification of fish disease pathogens

A universal primer PCR(UPPCR), PCR RFLP and PCR SSCP had been screened for a molecular biological method that permitted the rapid identification of fish pathogens among them. The results showed that a universal primer PCR technology was available with specific primers from conserved regions of bacterial 16S ribosomal RNA genes. The bacteria tested included some common causative agents of fish diseases such as Aeromonas hydrophila, Yersinia ruckeri, Vibrio anguillarum, Flexibacter columnaris, beta Streptococcus, Pseudomonas fluorescens. The approach allowed the bacteria above and Escherichia coli and Bifidobacterium catenulatum detectable without any alterations of the experiment conditions. However, this study also found that the identification of species of bacteria tested depended on the combination of UPPCR and SSCP(PPCR SSCP), which was better than that of UPPCR and RFLP(UPPCR RFLP) and made rapid diagnosis of fish disease pathogens possible.