Miracle-dz/UniFAR-FTUs huggingface dataset:
Gradient Microstructure in a Gear Steel Produced by Pressurized Gas Nitriding

A tempered martensitic gear steel (18CrNiMo7-6) sample was nitrided on two sides using a 5 atm pressurized gas at 530 °C for five hours. The nitrided sample was characterized by means of microhardness, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. A microhardness gradient was identified over a distance of 1000 µm with hardness values from 900 HV0.1 at the surface to 300 HV0.1 in the center matrix. The gradient microstructure was mainly divided into three zones: (i) a nitride compound layer at the top surface (~20 µm thick), (ii) a diffusion zone with containing precipitates (~350 µm thick), and (iii) the center matrix of the tempered martensite. Compared with carburized sample, the harder surface of the nitrided one ensures a better performance of the present pressured gas nitrided gears.
Effects of the Diet Inclusion of Common Vetch Hay Versus Alfalfa Hay on the Body Weight Gain, Nitrogen Utilization Efficiency, Energy Balance, and Enteric Methane Emissions of Crossbred Simmental Cattle

Simple Summary Nitrogen utilization efficiency and enteric methane emission from ruminants remain the primary concerns when developing ruminant feed globally. Nitrogen utilization efficiency is the ratio of retained nitrogen in body tissue to the total nitrogen intake, which is the main factor in the body weight gain of ruminants, and usually range from 15% to 40%. The methane emissions of ruminants are an inevitable by-product when feeds have been fermented in the rumen and represents a 2% to 12% loss of diet energy. The low nitrogen utilization of ruminants can damage air quality and lead to soil nitrification and acidification, whereas high methane emissions from ruminants can increase global warming. Our study investigated the effects of two kinds of legumes (alfalfa and common vetch) with different levels (20% vs. 40%) of total dry matter allowance on body weight gain, nutrient digestibility, nitrogen utilization efficiency, and enteric methane emissions for crossbred Simmental cattle. Our results suggested that nitrogen utilization efficiency and methane emissions are significantly affected by the legume species and proportions. These results could be beneficial for the development of regional or national ruminant feeding systems, thereby improving nitrogen utilization efficiency and reducing methane emissions. Abstract A low nitrogen utilization efficiency (NUE, the ratio of retained N to N intake) and high methane (CH4) emissions of ruminants can lead to potentially high diet protein wastage and directly contribute to global warming. Diet manipulation is the most effective way to improve NUE or reduce CH4 emissions. This study investigated how replacing oat hay with alfalfa hay (AH) or common vetch hay (CVH) with different proportions (20% (20) and 40% (40) of the total dry matter (DM) allowance) affects the body weight gain (BWG), NUE, and CH4 emissions of crossbred Simmental cattle. The forage dry matter intake (DMI) and the total DMI of cattle fed on a CVH40 diet were significantly higher than the values for those fed on AH20 or AH40 diets (p < 0.05). There were no differences in the BWG for the four treatments observed, however, nutrient digestibility significantly decreased in the AH40 diet as compared with the AH20 diet (p < 0.05). The NUE was significantly lower in AH40 than in CVH20. The CH4 emissions were significantly lower for the CVH40 diet than with the AH20 diet (p < 0.05). Our findings suggest that a 20% AH and 40% CVH substitution for oat hay are the optimal proportions to maintain the BWG, NUE, nutrient digestibility, and reduce the CH4 emissions of crossbred Simmental cattle. Overall, CVH has a greater potential to reduce CH4 emissions than AH.
A new resolution function to evaluate tree shape statistics

Phylogenetic trees are frequently used in biology to study the relationships between a number of species or organisms. The shape of a phylogenetic tree contains useful information about patterns of speciation and extinction, so powerful tools are needed to investigate the shape of a phylogenetic tree. Tree shape statistics are a common approach to quantifying the shape of a phylogenetic tree by encoding it with a single number. In this article, we propose a new resolution function to evaluate the power of different tree shape statistics to distinguish between dissimilar trees. We show that the new resolution function requires less time and space in comparison with the previously proposed resolution function for tree shape statistics. We also introduce a new class of tree shape statistics, which are linear combinations of two existing statistics that are optimal with respect to a resolution function, and show evidence that the statistics in this class converge to a limiting linear combination as the size of the tree increases. Our implementation is freely available at https://github.com/WGS-TB/TreeShapeStats.
Dual-Band Dual-Polarized Wearable Button Array With Miniaturized Radiator

A dual-band dual-polarized wearable array is proposed, based on a miniaturized innovating button radiator topology. The diameter of the rigid button is only 19.5 mm (0.29 λ at 4.5 GHz), which optimizes the users’ comfort, and makes it the smallest up to date in literature. The operational bands are 4.50–4.61 GHz and 5.04–5.50 GHz. The antenna thus covers the 4.5–4.6 unlicensed future 5th generation (5G) communication band for the internet of things (IoT), and the 5.1–5.5 GHz wireless local area network (WLAN) band, respectively. Two orthogonal linear polarizations are obtained in each band. A low mutual coupling between the button antenna elements (below −18 dB) and between the two ports within each element (below −20 dB) is achieved, guaranteeing a good diversity performance. The envelope correlation coefficient (ECC) and the specific absorption rate (SAR) performance are also analyzed. In order to demonstrate the robustness of the button antenna and to mimic realistic situations, a more complicated asymmetrical ground plane model of the button antenna is studied for the first time. A prototype of a two-element button array has been fabricated. The measurement results match well with the simulations. A 10-element button array is studied within the context of a 3-D channel model, taking into account the button element's radiation pattern. A high achievable spectral efficiency (SE) is obtained.
Efficacy of alkyltrimethylammonium bromide for decontaminating salt‐cured hides from the red heat causing moderately halophilic bacteria

Conventionally, animal hide and skin necessitates 95% saturated brine solution (SBS) for its preservation. This salt is primarily derived from different sources including solar‐saltern, evaporation ponds, etc., which are laden with different types of halophilic micro‐organisms. Previous studies confirmed that the presence of moderately halophilic bacteria caused red heat on cured hide, which adversely affects the leather quality and causes substantial economic losses for leather industries. Thus, this investigation was carried out to examine the effects of different concentrations of alkyltrimethylammonium bromide (ATMB) on selected halophilic‐bacteria attributed to the deterioration of hide quality. In nutrient broth solution (NBS), ATMB at 250 and 500 ppm reduced individual halo‐bacteria, that is, Halomonas halodenitrificans, Halomonas eurihalina, Alkalibacillus haloalkaliphilus and Salimicrobium album, by averages of 0·64 and 1·90, 1·5 and 2·61, 0·90 and 2·27, 1·65 and 3·36 log CFU per ml respectively in 5 min. ATMB treatment in SBS at 500 ppm for 18 h resulted in a reduction of H. halodenitrificans, H. eurihalina, A. haloalkaliphilus and S. album by averages of 1·9, 1·25, 0·96 and 1·34 log CFU per ml respectively, when compared with the controls. Likewise, 5000 ppm ATMB reduced the cocktail population nearly to zero from that cultivated in SBS for 18 h.
A World of Gorse: Persistence of Ulex europaeus in Managed Landscapes

Gorse (Ulex europeus L.) is a woody legume and invasive woody weed that has been introduced to temperate pastoral landscapes worldwide. Despite the apparent cosmopolitan distribution of gorse across much of the temperate agroecological landscapes of the world, research and practice pertaining to the management of gorse has been largely constrained to single-treatments, regions, or timeframes. Gorse eradication has been widely attempted, with limited success. Using the PRISMA (preferred reporting items for systematic reviews and meta-analysis) method and a quasi-metanalytical approach, we reviewed the seminal ~299 papers pertaining to gorse management. We identified (i) the ecological characteristics of the species that predispose gorse to behaving invasively, and (ii) the success of management actions (from a plant ecological life history perspective) in reducing weed vigour and impact. A broad ecological niche, high reproductive output, propagule persistence, and low vulnerability to pests allow for rapid landscape exploitation by gorse throughout much the world. Additionally, there are differences in flowering duration and season in the northern and southern hemisphere that make gorse particularly pernicious in the latter, as gorse flowers twice per year. The implications of these life history stages and resistance to environmental sieves after establishment are that activity and efficacy of control is more likely to be favourable in juvenile stages. Common approaches to gorse control, including herbicides, biological controls, and fire have not been ubiquitously successful, and may in fact target the very site resources—sward cover, soil stability, hydrological balance—that, when degraded, facilitate gorse invasion. Ongoing seedling regeneration presents difficulties if eradication is a goal, but facilitated competition may reduce costs via natural suppression. Mechanical methods of gorse removal, though highly successful, induce chronic soil erosion and land degradation and should hence be used sparingly.
Interactive Effects of Salicylic Acid and Nitric Oxide in Enhancing Rice Tolerance to Cadmium Stress

Cadmium (Cd) is one of the prominent environmental hazards, affecting plant productivity and posing human health risks worldwide. Although salicylic acid (SA) and nitric oxide (NO) are known to have stress mitigating roles, little was explored on how they work together against Cd-toxicity in rice. This study evaluated the individual and combined effects of SA and sodium nitroprusside (SNP), a precursor of NO, on Cd-stress tolerance in rice. Results revealed that Cd at toxic concentrations caused rice biomass reduction, which was linked to enhanced accumulation of Cd in roots and leaves, reduced photosynthetic pigment contents, and decreased leaf water status. Cd also potentiated its phytotoxicity by triggering reactive oxygen species (ROS) generation and depleting several non-enzymatic and enzymatic components in rice leaves. In contrast, SA and/or SNP supplementation with Cd resulted in growth recovery, as evidenced by greater biomass content, improved leaf water content, and protection of photosynthetic pigments. These signaling molecules were particularly effective in restricting Cd uptake and accumulation, with the highest effect being observed in “SA + SNP + Cd” plants. SA and/or SNP alleviated Cd-induced oxidative damage by reducing ROS accumulation and malondialdehyde production through the maintenance of ascorbate and glutathione levels, and redox status, as well as the better activities of antioxidant enzymes superoxide dismutase, catalase, glutathione S-transferase, and monodehydroascorbate reductase. Combined effects of SA and SNP were observed to be more prominent in Cd-stress mitigation than the individual effects of SA followed by that of SNP, suggesting that SA and NO in combination more efficiently boosted physiological and biochemical responses to alleviate Cd-toxicity than either SA or NO alone. This finding signifies a cooperative action of SA and NO in mitigating Cd-induced adverse effects in rice, and perhaps in other crop plants.
In Vivo Photoacoustic Sentinel Lymph Node Imaging Using Clinically-Approved Carbon Nanoparticles

Objective: Breast cancer is the most common type of invasive cancer and one of the leading causes of cancer death in women worldwide. Correct staging of breast cancer is critical to the survival rate of the patients. Sentinel lymph node (SLN) biopsy (SLNB), currently the gold standard technique for breast cancer staging, requires preoperative and intraoperative image guidance for noninvasive SLN identification and minimal surgical invasion. However, existing image guidance techniques suffer from a variety of limitations, such as ionizing radiation, high cost, and poor imaging depth. To address the clinical challenges, new methodology has to be developed. Methods: We developed a photoacoustic (PA) imaging procedure for noninvasive and nonradioactive SLN identification and biopsy guidance enhanced with a clinically-approved lymphatic tracer, i.e., carbon nanoparticles (CNPs) suspension injection. Results: In vivo experiments show that the proposed procedure could sensitively identify the SLN and provide high-contrast image guidance for fine-needle aspiration simulation. In addition, we demonstrated that CNPs have significantly better performance than other commonly-used contrast agents, such as methylene blue and indocyanine green. Conclusion: PA imaging technique using clinically-approved CNPs as the contrast agent is capable for noninvasive and nonradioactive SLN identification and high-contrast biopsy guidance, and should be considered as a new tool for assisting SLNB in breast cancer staging. Significance: The proposed CNPs-enhanced PA imaging technique provides a practical way for SLN identification and biopsy guidance for breast cancer patients and paves the way for clinical translation of PA SLN imaging.
Structure of an RNA aptamer in complex with the fluorophore tetramethylrhodamine

Abstract RNA aptamers—artificially created RNAs with high affinity and selectivity for their target ligand generated from random sequence pools—are versatile tools in the fields of biotechnology and medicine. On a more fundamental level, they also further our general understanding of RNA-ligand interactions e. g. in regard to the relationship between structural complexity and ligand affinity and specificity, RNA structure and RNA folding. Detailed structural knowledge on a wide range of aptamer–ligand complexes is required to further our understanding of RNA–ligand interactions. Here, we present the atomic resolution structure of an RNA–aptamer binding to the fluorescent xanthene dye tetramethylrhodamine. The high resolution structure, solved by NMR-spectroscopy in solution, reveals binding features both common and different from the binding mode of other aptamers with affinity for ligands carrying planar aromatic ring systems such as the malachite green aptamer which binds to the tetramethylrhodamine related dye malachite green or the flavin mononucleotide aptamer.
Overexpression of BUNDLE SHEATH DEFECTIVE 2 improves the efficiency of photosynthesis and growth in Arabidopsis

Summary Bundle Sheath Defective 2, BSD2, is a stroma‐targeted protein initially identified as a factor required for the biogenesis of ribulose 1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) in maize. Plants and algae universally have a homologous gene for BSD2 and its deficiency causes a RuBisCO‐less phenotype. As RuBisCO can be the rate‐limiting step in CO2 assimilation, the overexpression of BSD2 might improve photosynthesis and productivity through the accumulation of RuBisCO. To examine this hypothesis, we produced BSD2 overexpression lines in Arabidopsis. Compared with wild type, the BSD2 overexpression lines BSD2ox‐2 and BSD2ox‐3 expressed 4.8‐fold and 8.8‐fold higher BSD2 mRNA, respectively, whereas the empty‐vector (EV) harbouring plants had a comparable expression level. The overexpression lines showed a significantly higher CO2 assimilation rate per available CO2 and productivity than EV plants. The maximum carboxylation rate per total catalytic site was accelerated in the overexpression lines, while the number of total catalytic sites and RuBisCO content were unaffected. We then isolated recombinant BSD2 (rBSD2) from E. coli and found that rBSD2 reduces disulfide bonds using reductants present in vivo, for example glutathione, and that rBSD2 has the ability to reactivate RuBisCO that has been inactivated by oxidants. Furthermore, 15% of RuBisCO freshly isolated from leaves of EV was oxidatively inactivated, as compared with 0% in BSD2‐overexpression lines, suggesting that the overexpression of BSD2 maintains RuBisCO to be in the reduced active form in vivo. Our results demonstrated that the overexpression of BSD2 improves photosynthetic efficiency in Arabidopsis and we conclude that it is involved in mediating RuBisCO activation.
Adaptation of Mammalian Cells to Chemically Defined Media

In order to circumvent ethical, technical, and economic drawbacks regarding the use of animal serum in cell culturing, it is possible to adapt mammalian cells to serum‐free media. Nowadays, there are several serum‐free formulations available, including fully animal derived–free and chemically defined media, and different adaptation techniques. This article focuses on the gradual adaptation of a mammalian suspension cell culture to a chemically defined medium. The first step is to transfer the cells cultured in medium supplemented with fetal bovine serum (FBS) to a chemically defined medium of your choice, containing the same amount of FBS. The next steps consist of progressively reducing the amount of FBS, while monitoring cell growth and viability up to the complete elimination of FBS. This protocol has been successfully used to adapt THP‐1 cells to a chemically defined medium with similar maximum specific growth rate as those cultured with FBS. © 2019 by John Wiley & Sons, Inc.
Enhancement of zaleplon oral bioavailability using optimized self-nano emulsifying drug delivery systems and its effect on sleep quality among a sample of psychiatric patients

Abstract The aim of this work is to develop self-nano emulsifying drug delivery system (SNEDDS) to enhance the oral bioavailability of zaleplon (Zal) as a poorly water-soluble drug. Moreover, the bioavailability and the effect on the quality of sleep among a sample of psychiatric patients is to be assessed. D-optimal mixture design was used for optimization. Optimized SNEDDS formulation was evaluated for droplet size, transmission electron microscope (TEM) and in-vitro dissolution test. Zal bioavailability was evaluated by determining its serum concentration and pharmacokinetic parameters in 8 patients after oral administration. Effect on sleep quality was assessed among 40 psychiatric patients. Patients’ sleep quality was assessed in 40 psychiatric patients before and after medication using the Arabic version of the Pittsburgh Sleep Quality Index (PSQI). Zal- SNEDDS appeared as nano-sized spherical vesicles. Moreover, Zal was completely dissolved from optimized formulation after 45 min indicating improved dissolution rate. Zal-SNEDDS showed significantly higher C max, T max and AUC 0→∞ compared to commercial product after oral administration. Zal-SNEDDS significantly improved the total score of PSQIs (p < .001) with higher subjective sleep quality, reduced sleep latency, improved day time function and sleep disturbance (p < .001). Using sleep medication was reduced significantly (p = .027). However, it did not modify sleep duration or sleep efficiency. SNEDDS have improved Zal solubility and enhanced its bioavailability. Furthermore, Zal-SNEDDS have improved the total score of PSQIs and may be considered a good choice to enhance the quality of sleep among psychiatric patients.
Effect of Herbals Tulsi and Turmeric on Cortisol, Enzymatic and Biochemical constituents in Serum of Heat stressed Broilers

An experiment of 42 days was taken up to study the efficacy of herbal antioxidants Tulsi (Ocimum sanctum) and Turmeric (Curcuma longa) in reducing heat stress induced changes in serum constituents during summer months. 168 birds (day old) were divided into 7 groups (24 each) in completely randomized design. Basal diet (BD) was offered to G-I (Heat stressed (HS)) without any antioxidant supplementation. The herbals Tulsi and Turmeric were supplemented solely at concentrations of 0.25%, 0.5% and 0.2, 0.4% to groups II, III, IV and V, respectively and as combinations (0.25% Tulsi + 0.2%Turmeric and 0.5% Tulsi + 0.4% Turmeric) to groups VI and VII, respectively. Additionally 24 day old chicks were raised separately in stress free environment that served as the control group. The blood samples were collected at 4th and 6th weeks of age and estimated for cortisol concentration, alkaline phosphatase (ALP) and alkaline transaminase (ALT) enzymes and protein profiles (Total protein (TP),albumin and globulin) in serum. The serum ALP (1430 and 1849 IU/l at 4thand 6th wk, respectively) ALT (17.74 and 51.42 IU/L at 4th and 6th wk, respectively) and cortisol (4.54 and 8.77 μg/dl at 4th and 6th wk, respectively) were higher (P≤0.01) in HS group and lowest in stress free group. While, TP, albumin and globulin concentrations were significantly (P≤0.01) lower in HS group compared to the control group. Supplementation of BD with Tulsi and Turmeric lowered (P≤0.01) the activity of serum enzymes (ALP and ALT) and cortisol concentration and increased the TP, albumin and globulin concentration compared to HS birds. Herbals in combination supplemented at higher concentration (0.5% Tulsi and 0.4% Turmeric) showed significant (P≤0.01) improvement than when supplemented at lower concentration (0.25 and 0.2%, respectively). However, combination of herbals at either doses did not show any additive advantage than their sole inclusions. Tulsi and Turmeric supplemented at 0.5 and 0.4%, respectively were effective in mitigating the changes in serum constituents induced due to heat stress.
Novel Epigenetic Biomarkers in Pregnancy-Related Disorders and Cancers

As the majority of cancers and gestational diseases are prognostically stage- and grade-dependent, the ultimate goal of ongoing studies in precision medicine is to provide early and timely diagnosis of such disorders. These studies have enabled the development of various new diagnostic biomarkers, such as free circulating nucleic acids, and detection of their epigenetic changes. Recently, extracellular vesicles including exosomes, microvesicles, oncosomes, and apoptotic bodies have been recognized as powerful diagnostic tools. Extracellular vesicles carry specific proteins, lipids, DNAs, mRNAs, and miRNAs of the cells that produced them, thus reflecting the function of these cells. It is believed that exosomes, in particular, may be the optimal biomarkers of pathological pregnancies and cancers, especially those that are frequently diagnosed at an advanced stage, such as ovarian cancer. In the present review, we survey and critically appraise novel epigenetic biomarkers related to free circulating nucleic acids and extracellular vesicles, focusing especially on their status in trophoblasts (pregnancy) and neoplastic cells (cancers).