Miracle-dz/UniFAR-FTUs huggingface dataset:
Long term effects of fire frequency and season on the woody vegetation dynamics of the Sclerocarya birrea/Acacia nigrescens savanna of the Kruger National Park

A lack of knowledge together with vacillating fire management approaches in the Kruger National Park until the mid 1950s, gave rise to a long term fire research experiment aimed at shedding light on savanna responses to various combinations of fire fre- quencies and seasons. This trial was laid out in 1954 in four of the six major vegetation zones of the park. With the future of the experiment now being reconsidered, full scale vegetation surveys have been conducted on all the plots and compared to the surveys done in 1954. This paper examines the woody vegetation responses to fourteen fire treatments in the Knobthorn/Marula savanna. Parameters of interest were woody species composition responses, together with tree & shrub density and structural changes. The results indicate that no significant changes in woody species had occurred for the peri- od 1954 vs 1998, while density decreased on biennial and increased on triennial treatments. The proportion of single stemmed plants increased over the period. Season of burn has a marked effect on structure, with April and August burns giving rise to the largest basal areas but the lowest heights. Environmental parameters such as climate, varying herbivory and differing soils, and their respective interactions on vegetation morphology, together with fire behaviour, further influenced results.
LincRNA-p21 promotes classical macrophage activation in acute respiratory distress syndrome by activating NF-κB

Abstract Background: Previous studies have revealed the important role of alveolar macrophages (AMs) in the pathogenesis of acute respiratory distress syndrome (ARDS) and potential anti-inflammatory properties of lincRNA-p21. This study aims to study the association between lincRNA-p21 and active AMs to understand the molecular mechanisms of AMs-mediated inflammatory responses in ARDS. Methods: This study was mainly investigated in mice with the intratracheal instillation of lipopolysaccharide (LPS) or LPS-treated AMs. The expression of lincRNA-p21 and classical macrophage markers, IL-12β and iNOS, was detected by quantitative RT-PCR, while NF-κB p65 translocation was measured by western blotting analysis. And, NF-κB activity was analyzed through luciferase report assays. Gain- and loss-of-function studies were also performed for further investigations. Results: Elevated lincRNA-p21 levels were observed in both LPS-induced ARDS mice and LPS-treated AMs, with upregulated expression of IL-12β and iNOS, namely M1 activation, and p65 nuclear translocation. Further in vitro studies showed that LPS-induced M1 activation could be counteracted by both lincRNA-p21 inhibition and inhibited NF-κB activation. Moreover, both p65 nuclear translocation and NF-κB activity were promoted by lincRNA-p21 overexpression, while lincRNA-p21 inhibition showed a negative effect on LPS-induced p65 nuclear translocation and increase of NF-κB activity. Additionally, LPS-induced lung injuries could be attenuated by lincRNA-p21 inhibition in vivo. Conclusion: This study revealed elevated lincRNA-p21 levels in LPS-induced ARDS and investigated the potential role of lincRNA-p21 in LPS-induced pro-inflammatory response via NF-κB/p65 mediated pathways, suggesting the potential application of lincRNA-p21 for ADRS therapy.
Women Convicted of Intimate Partner Homicide in Oromiya Regional State of Ethiopia: Profile and Associated Factors

This study explored the factors associated with the engagement of women in intimate partner homicide (IPH) through 10 in-depth interviews with women incarcerated in three correction facilities in Oromiya Regional State of Ethiopia. In addition, key informant interviews and six focus groups involving traditional leaders, Kebele administrators, and community members help triangulate the findings. The study revealed insight into women’s social, psychological, and economic status prior to their crime. Low levels of education and economic opportunity, forced marriage, marital dispute due to polygamy, spousal violence, and low levels of community support were associated with IPH. Implications for research, policy, and practice are discussed.
An improved method for isolation of fertile zona‐free mouse eggs

Studies of sperm-egg fusion using zona-free mouse eggs are impaired by the procedures used for removal of the zona pellucida. Methods involving proteolytic digestion or mechanical aspiration through micropipettes are limited in that proteases can adversely affect fertility and mechanical removal often results in low egg yields. An efficient procedure for preparation of zona-free mouse eggs was developed using a combined enzymatic (chymotrypsin) mechanical approach (CT-M procedure). Zona-intact eggs, obtained after hyaluronidase treatment, were exposed to 0.001% α-chymotrypsin in medium containing 0.5% bovine serum albumin (BSA). Brief (2 minute) exposure to chymotrypsin under these conditions caused pronounced zona distention in a majority (80-90%) of the eggs, facilitating mechanical removal and resulting in a high yield of zona-free eggs. Eggs prepared by the CT-M method displayed identical penetration levels relative to mechanically denuded eggs. CT-M prepared eggs also showed sperm concentration dependent penetration levels and demonstrated a plasma membrane block to polyspermy, qualities previously observed in mechanically prepared eggs [Wolf DP, 1978, Dev Biol 64:1–10]. Eggs could be exposed to 0.001% CT for zona distention over a 2-10-minute time period with no detrimental effects on fertility. The effect of chymotrypsin was also studied by treating zona-free eggs for 30 minutes over a 1-1,000–μg/ml range of enzyme, and a concentration-dependent reduction in penetration levels was observed. These results indicate that the CT-M method is a useful procedure for the isolation of large numbers of zona-free mouse eggs.
Regular Coffee Consumption Is Associated with Lower Regional Adiposity Measured by DXA among US Women.

BACKGROUND Coffee is among the most popular daily beverages in the United States. Importantly, coffee consumption has been associated with a lower risk of multiple health outcomes including a reduction in adiposity. DXA is a means to assess body fat and distribution. OBJECTIVES The aim of this study was to examine the relation between coffee consumption and DXA-assessed adiposity and adiposity distribution. METHODS Cross-sectional data from the NHANES were used. Participants were adults aged 20-69 y from the 2003-2004 and 2005-2006 waves. Information on coffee consumption was assessed through the FFQ (categorized as no coffee, 0 to <0.25 cup/d, 0.25 to <1 cup/d, 1 cup/d, 2-3 cups/d, or ≥4 cups/d). Both caffeinated and decaffeinated coffee consumption were included. Trunk fat and total fat percentage were measured via whole-body DXA scans. The association between coffee consumption and body fat was investigated using age-adjusted and multivariable-adjusted linear regression models which accounted for sample weights. RESULTS Higher coffee consumption was associated with significantly lower total body fat percentage and trunk body fat percentage in a dose-response manner (all P values < 0.05) among women. Although this dose-response relation was nonsignificant among men, men aged 20-44 y who drank 2-3 cups/d had 1.3% (95% CI: -2.7%, 0.1%) less total fat and 1.8% (95% CI: -3.3%, -0.4%) less trunk fat than those who did not consume coffee. Furthermore, the association between coffee consumption and body fat percentage exhibited for both caffeinated and decaffeinated coffee among women (all P for trend < 0.001) but not among men (all P for trend > 0.05). CONCLUSIONS The present study found a significant association between higher coffee consumption and lower DXA-measured adiposity. Moreover, a gender difference in this association in the general US adult population was also observed.
Transgenic wheat with enhanced fungal resistance causes no effects on Folsomia candida (Collembola : Isotomidae)

Summary The potential environmental impact of genetically modified (GM) plants is a major concern arising from the use of these novel crops. Therefore an ecological risk assessment should be done prior to the wide scale use of a GM crop. Soil microarthropods are a group of non-target organisms that should be considered as part of this risk assessment due to their importance for the decomposition process of organic matter and due to their intense exposure to crop residues in the soil. In the study described here, two different transgenic wheat varieties were used that express a gene from an Ustilago maydis -infecting virus. The gene product (KP4 protein) is known for its growth inhibitory activity against fungi in the Ustilaginales. These KP4-transgenic wheat plants show an enhanced resistance against stinking smut, Tilletia tritici . We describe different laboratory bioassays and a glasshouse study where we tested effects of the two transgenic KP4-wheat varieties on the Collembola Folsomia candida . Feeding bioassays in which dried root material from transgenic and non-transgenic wheat plants was fed to individual F. candida revealed no effect of transformation or wheat variety on any of the life-history parameters evaluated. The comparison to an optimal food source (yeast) showed that certain parameters, i.e. insect development, egg cluster size and insect weight, are very sensitive parameters to detect differences in food quality. Other parameters including egg development and egg viability, revealed no difference between plant material and yeast fed F. candida . The glasshouse study showed that population development of F. candida did not differ between pots in which transgenic or non-transgenic plants of the two varieties were grown. However, a significant variety effect was detected.
On the function of flowers

Most flowers are bisexual in function, but counting secondary allocation to attractive structures such as the corolla as equally male and female leads to the paradoxical conclusion that plants bearing perfect flowers invariably allocate much more to female than to male function. A method of calculating the gender of secondary floral allocation is described, and it is speculated that this allocation is predominantly male. Observations and experiments with natural populations of herbs, designed to test this hypothesis gave the following major results, (i) Insects visit larger flowers more frequently (Fragaria), and removal of floral biomass causes a reduction in the frequency of insect visits proportional to the fraction of biomass removed (Impatiens). (ii) Removal of attractive structures may cause a decline in the probability that a fruit will be formed but has no effect on the number of seeds set per fruit; thus, mutilation of essentially solitary flowers has no effect on seed-set per fruit (Impatiens), while removal of flowers from inflorescences in a species that forms several many-seeded fruits per inflorescence reduces fruit-set per inflorescence but has no effect on seed-set per fruit (Asclepias), and removal of sterile flowers from an inflorescence in which the fertile flowers yield one-seeded fruits is effective in reducing seed-set per inflorescence (Viburnum), (iii) Larger flowers may disperse a greater fraction of their pollen in unit time (Impatiens) and the removal of flowers from inflorescences causes a steep reduction in total pollen exported and a weak decline in the quantity of pollen exported per flower (Asclepias). These results are consistent with the hypothesis that a single insect visit (or a very few visits) suffices to fertilize almost all available ovules and is procured by a very small allocation to attractive structures, while much greater allocation is necessary to procure the numerous visits required to disperse a large fraction of the pollen. This inference is supported by a comparative survey of sexually dimorphic plants, in which male flowers are generally larger than female flowers, male inflorescences bear more flowers, and male plants bear more inflorescences. It is concluded that the flower is primarily a male organ, in the sense that the bulk of allocation to secondary floral structures is designed to procure the export of pollen rather than the fertilization of ovules. This conclusion may be sensitive to whether it is the flower or the inflorescence as a whole that represents the primary unit of attraction to insects. It was found that the performance of a given flower was substantially affected by other flowers in the same compact inflorescence (Asclepias), though not by other inflorescences on the same plant nor by those borne by nearby plants (Fragaria, Impatiens). A general quantitative theory of flower and inflorescence design is outlined, and used to organize the extensive experimental results for Asclepias.
Conventional weight loss interventions across the different BMI obesity classes: A systematic review and quantitative comparative analysis.

OBJECTIVE The recommendation for conventional body weight loss (BWL) treatment in obesity is 5-10%. It is not clear whether BWL is similar across the three different body mass index (BMI) obesity classes. The aim was to provide an overview on BWL across these classes in moderate lifestyle/diet intervention programs. METHOD A systematic literature search was conducted and the evidence of randomized controlled trials (RCTs) and pre-post design studies synthesized. The outcome was BWL. RESULTS For RCTs, mean BWL in the intervention group was 3.6 kg (class I) and 5.3 kg (class II), which equates to 4 and 5% BWL, respectively. None of the assessed class III obesity studies met the inclusion criteria. For pre-post design studies, mean BWL was 5.4 kg (class I), 5.5 kg (class II) and 7.9 kg (class III), with high variation within and across studies in the latter. This equates to 6, 5 and, 6% BWL, respectively. CONCLUSIONS BWL of moderate BWL programs are similar across the different obesity classes. For class I obesity, the results differ between RCT and pre-post design studies by 2% BWL. The high variation of BWL in class III obesity might reflect different states of motivation such as the attitude towards bariatric surgery.
Differences in MC4R mRNA levels between Casertana and Large White pig breeds

Riassunto Differenze tra i livelli di mRNA del gene MC4R nelle razze suine Casertana e Large White. Allo scopo di analizzare differenze nei livelli di espressione genica tra due razze suine a fenotipo estremo è stata costruita una popolazione sperimentale composta da 17 soggetti di razza Casertana e 16 di razza Large White. Impiegando l’approccio dei geni candidati è stato scelto e studiato il gene “Recettore-4 della Melanocortina”, importante nella via metabolica che regola omeostasi energetica ed appetito. L’analisi è stata condotta utilizzando la tecnica della Real-Time PCR impiegando il gene della “Gliceraldeide 3-Fosfato Deidrogenasi” come riferimento. L’ RNA totale è stato isolato da tessuto muscolare campionato al macello. Le analisi hanno evidenziato una grande variabilità individuale nell’espressione relativa del gene in ambo le razze (es.: valori di espressione compresi in un range di 0,05÷11,08 in Casertana e di 0,44÷4,86 in Large White). Inoltre è stata evidenziata una differenza significativa di espressione tra i campioni delle due razze considerate, risultato che però va verificato su un numero maggiore di campioni.
Cerebrospinal fluid-contacting neurons affect the expression of endogenous neural progenitor cells and the recovery of neural function after spinal cord injury

Abstract Objective To explore the relationship between cerebrospinal fluid-contacting neurons (CSF-cNs) and endogenous neural progenitor cells (ENPCs) and whether CSF-cNs are involved in nerve repair after spinal cord injury (SCI). Methods Cholera toxin B-horseradish peroxidase complex (CB-HRP) and cholera toxin B conjugated with saporin (CB-SAP) were injected into the lateral ventricles of spinal cord injured rats to mark and destroy the CSF-cNs. Then the rats in the experimental group were injured by SCI. Observe the content and co-expression of CSF-cNs and ENPCs in rats of each group, and observe the recovery of motor function after SCI in each group. Results After the destruction of CSF-cNs, the number of ENPCs decreased significantly in the long term after the surgery, and the recovery of motor function also deteriorated as compared to the group with intact CSF-cNs. Meanwhile some cells in the spinal cord express both the biological marker of CSF-cNs and ENPCs. Conclusion This study shows that the population of ENPCs and motor function recovery in SCI rats declined after the destruction of CSF-cNs, suggesting that CSF-cNs affect the ENPCs population and may be involved in the recovery of neural function after SCI.
School Principals Putting Bullying Policy to Practice

School principals must rely on state statutes and district policies to navigate reports of school bullying. Investigating reports to determine the outcomes may vary depending upon the districts definition of bullying, the investigation process, and follow through to reporting the findings of the investigation to the involved children and youth’s parents. However, investigating reports can be challenging due to the confusion of what constitutes bullying. This confusion can be especially troubling for parents who believe their child is being bullied. In order to understand principals' perspectives on bullying, two focus groups were conducted with nine urban school principals. Researchers examined principals’ perceptions of how state- and district-level policies were used within their bully investigation practices. These principals suggest that a clear, specific district wide definition of bullying and step by step procedures to investigate reports, along with the state anti-bullying statute, provide a valuable guide for follow-through and back up in determining cases of school bullying. In addition, they identified how policies and district mandates affected parents, particularly when reporting their investigation findings. Implications for bully prevention policies are discussed.
On the Effects of Constitutive Properties and Roughness of a Hard Inclusion in Soft Tissue on B-mode Images

We perform finite element modeling of pulse-echo ultrasound of a hard inclusion in a soft tissue to gain a better understanding of B-mode image brightness characteristics. We simulate a pressure wave emitted by an ultrasound transducer through the inclusion-tissue medium by prescribing suitable boundary conditions, and collect the scattered wave response to simulate the behavior of the transducer array used for pulse-echo ultrasound. We form B-mode images from simulated channel data using standard delay and sum beamforming. We establish the accuracy of the finite element model by comparing the point spread function with that obtained from Field II ultrasound simulation program. We also demonstrate qualitative validation by comparing the brightness characteristics of rough and smooth surfaced circular inclusions with experimental images of a cylindrical metal tool immersed in a water tank. We next conduct simulation studies to evaluate changes in B-mode image brightness intensity and contrast related to different constitutive properties, namely, compressibility of the inclusion, impedance contrast between the host and inclusion, and surface roughness of the inclusion. We find that the intensity observed behind a hard inclusion in the axial direction is strongly affected by the compressibility and roughness of the inclusion. Also, the perceived width of the stone based on the intensity is greater for rougher stones. Our study indicates that imaging of compressible inclusions may benefit from targeted B-mode image forming algorithms. Our modeling framework can potentially be useful in differentiating hard inclusions from surrounding parenchyma, and for classifying kidney stones or gallstones.
PHOSPHORUS, NITROGEN AND THE GROWTH OF ALGAE IN LAKE KINNERET 1

The intracellular concentrations of carbon, nitrogen, phosphorus and chlorophyll a of phytoplankton and zooplankton in Lake Kinneret were determined from 1969 to 1973. The ratios C:P, C:N, chlorophyll a:P, chlorophyll a:N of the algae showed fluctuations which could be related to the nutrient conditions that influence the annual pattern of phytoplankton development, especially in respect to the dinoflagellate bloom of Peridinium cinctum (OFM) Ehr. f. westii (Lemm.) Lef. Relatively high intracellurar P values at the start of the bloom indicated adequate availability of this nutrient and luxury consumption over a short period of time. Later, Peridinium continued to grow despite unusually high C:P ratios (> 300:1). In most years, phosphorus may have eventually limited growth, however, in 1970, the bloom censed despite comparatively high intracellular P concentrations. These observations, together with supplementary evidence from nutrient addition experiments and determinations of specific alkaline phosphatase levels, indicated that, for most of the growth phase of the bloom, Peridinium cells were not directly limited by P. The decline of the bloom usually, but not always (e.g., in 1970), was marked by very high C:P ratios. Thus, a shortage of P may often be a contributory factor to the cessation of the Peridinium bloom and may be limiting phytoplankton growth in the fall. Over the years 1969–73, possibly due to an overall drop in salinity, there appears to be a trend to lower levels of biologically bound phosphorus in Lake Kinneret, without a concomitant decrease in carbon biomass.
Phenolic lipids as unique bioactive compounds: a comprehensive review on their multifunctional activity toward the prevention of Alzheimer’s disease

Abstract Phenolic lipids are multifunctional compounds which play an important biological role in the body. Their unique biologic functionality stems from their strong amphiphilic character which allows them to be incorporated in erythrocytes. Through membrane incorporation, these compounds exert their biological effects on neurons which are not modulated by hydrophilic compounds. These bioactive compounds are present in nature as secondary plant metabolites, and consequently their availability is limited, for dietary and medical purposes. In this review, the pathways and mechanisms associated with the pathogenesis of Alzheimer’s disease will be described. In addition, the modulatory effects of phenolic lipids on these pathways and a list of several synthetic, semi synthetic and natural sources of phenolic lipids will be examined as having the potential to prevent or combat Alzheimer’s disease.
Understanding ethnic variations in HIV prevalence in Kenya: the role of cultural practices

Abstract Patterns of HIV prevalence in Kenya suggest that areas where various cultural practices are prevalent bear a disproportionate burden of HIV. This paper examines (i) the contextual effects of cultural practices (polygyny, male circumcision) and related sexual behaviour factors on HIV prevalence and (ii) the extent to which specific cultural practices in a community/county might explain existing ethnic variations in HIV prevalence in Kenya. The analysis applies multilevel logistic regression to data from the 2012/13 Kenya AIDS Indicator Survey. The results reveal striking ethnic variations in HIV prevalence in Kenya. The prevalence of polygyny in a community is positively associated with HIV prevalence, while a higher level of male circumcision in a county is protective for both men and women. The effects of these factors are stronger for men than women at both individual and contextual (community/county) levels. These cultural practices and associated risk factors partly explain existing ethnic differences in HIV prevalence in Kenya, but there remain significant ethnic variations that are not explained by these cultural practices or related sexual behaviour factors. These call for stronger empirical evidence to offer stronger theoretical explanations and inform effective policy and practice to address HIV epidemic in adversely affected communities in Kenya and similar settings in sub-Saharan Africa.