Miracle-dz/UniFAR-FTUs huggingface dataset:
LC-ESI-QTOF/MS Profiling of Australian Mango Peel By-Product Polyphenols and Their Potential Antioxidant Activities

Mango (Mangifera indica L.) is one of the most important fruits in the world. Mango peel is an important by-product that is rich in polyphenols and it could have high economic value if it is effectively utilized. Phenolic characterization is an essential step in the commercial utilization of mango peel by-products as food ingredients. Herein, qualitative and quantitative analyses of two Australian mango peel “Keitt” and “Kensington Pride” (K&P) by-products were conducted while using liquid chromatography coupled to electrospray ionisation and quadrupole time of flight mass spectrometry (LC-ESI-QTOF/MS) and high-performance liquid chromatography coupled to photodiode array detector (HPLC-PDA). A total of 98 polyphenols compounds were tentatively identified in both Keitt peel and K&P peel extracts, with greater concentrations of these compounds being detected in Keitt peel. The total phenolic content (TPC), total flavonoid content (TFC), and a total tannin content (TTC) were determined. The antioxidant activity of mango peel by-products was determined while using 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Keitt peel contained higher concentrations of total phenolic compounds, flavonoids, and tannins and had higher antioxidant capacity in DPPH, FRAP, and ABTS assays as compared to K&P peel. In HPLC-PDA quantification, the predominant phenolic compounds in Keitt peel and K&P peel were catechin (62.32 ± 0.01 mg/gd.w.) and syringic acid (17.78 ± 0.01 mg/gd.w).
Gadolinium at Low Concentration Suppresses both Osteoclastic and Osteoblastic Activities in the Scales of Goldfish

For determining the effect of environmental pollutants on fish bone metabolism, we have developed an in vitro bioassay system using teleost scales that has osteoclasts, osteoblasts and bone matrix as markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this bioassay, the influence of gadolinium (Gd) on osteoclasts and osteoblasts of goldfish scales was examined in the present study. Gd sensitively inhibited TRAP activity. Even Gd at 10-13 M suppressed TRAP activity at 6 hours of incubation. At 18 hours of incubation, this inhibition occurred only at 10-7 and 10-6 M. After 36 hours of incubation, Gd did not influence TRAP activity. In osteoblasts, ALP activity was also suppressed by Gd in the range of 10-10 to 10-6 M for 6 to 18 hours of incubation. At 36 and 64 hours of incubation, ALP activity was significantly suppressed by Gd (36 hours: 10-9 to 10-6 M; 64 hours: 10-7 and 10-6 M). At 96 hours of incubation, however, Gd did not influence ALP activity. This is the first report to indicate the toxicity of Gd on fish bone metabolism using TRAP and ALP enzyme activities. The toxicity of Gd to osteoblasts is comparable to that of tributyltin, an aquatic environmental pollutant used as a biocide in anti-fouling paint. Gd is used in Magnetic Resonance Imaging (MRI) for clinical diagnoses. To avoid the toxicity of Gd ions, chelated forms, known as Gd-based contrast agents, are used for MRI diagnosis. Without a specific recycling process, these compounds are quickly released by urinary excretion and released into environmental waters. Therefore, it is possible that anthropogenic Gd influences aquatic animals. Considering our present data together with that of anthropogenic Gd pollution, we should conduct a Gd risk assessment to protect the ecosystem in the aquatic environment.
A follow-up study on post myocardial infarction depression in a tertiary healthcare centre of Assam

Background: Major depressive disorder is widely prevalent in post myocardial infarction (MI) period. Various studies have reported a significant relationship between these two major disease states. Aim: To examine depression after MI and its socio-demographic variation. Methods: The study was a follow up study on patients of acute MI (n=50) attending cardiology outpatient department of the Assam Medical College Hospital in four to six weeks after the index event. Screening was done by the Primary Care Evaluation of Mental Disorders and diagnoses of major depressive disorder were established according to the text revision of the fourth edition of the Diagnostic and Statistical Manual for Mental Disorders criteria. Severity of the depression was assessed by the Beck Depression Inventory. Study populations were again reassessed after eight weeks from the index event. Statistical software packages like SPSS-20, XLSTAT, and Microsoft Office Access were used for analysis. Results: Twenty eight and 32% of the study subjects were depressed on their first and second visit respectively. Conclusion: Similar pattern of post MI depression is found in our state likewise in correlation to western and Indian studies which were conducted in different states. So we recommend vigilance from the clinician’s side while handling cases of MI.
Prognostic value of the time interval between surgery and adjuvant radiotherapyin patients treated for early stage invasive cervical cancer

A i m. Assessment of the prognostic value of the time interval between surgery and adjuvant postoperative radiotherapy. Material and methods . A retrospective analysis of 96 early stage cervical cancer patients, initially treated with surgery. All patients received postoperative radiotherapy in case of positive pelvic lymph nodes or inappropriate surgical margins. All patients received external beam radiotherapy, dose range 43.2 Gy and 46 Gy, and additionally, intracavitary brachytherapy – (LDR) with a mean dose 35 Gy, measured at 0.5 cm below the vaginal cylinder. Cox's proportional hazards model was performed to evaluate independent factors affecting survival. The incidence of treatment-related toxicity was also recorded and scored according to the RTOG/EORTC toxicity scale. The minimum follow-up was 60 months. Results . Among 96 patients in 5-year follow- up, 78 (81%) were still alive without evidence of disease. The 5-year disease free survival for the node negative group was 92%, and for the nodes positive group – 56% (p<0.0004). The analysis of prognostic value of the time interval between surgery and adjuvant radiotherapy for the group of 71 patients has demonstrated that the time interval of 32.5, 44.5, and over 60 days, resulted in a 5-year disease – free survival of 91%, 88% and 83% respectively. The time interval between surgery and radiotherapy over 60 days was also a significant factor negatively affecting survival (p<0.035). Conclusions . The results of our study confirm the role of the nodal status as the most important prognostic factor affecting the survival of patients treated surgically for stage Ib cervical carcinoma. For patients after non radical surgery the delay between surgery and radiotherapy is a statistically significant prognostic factor affecting survival.
Factors predicting the outcome of intravenous thrombolysis in stroke patients before rt-PA administration

Background: To determine whether it is possible to predict intravenous thrombolytic therapy (IVT) outcome after 3 months in acute ischemic stroke patients who are candidate to receive recombinant tissue plasminogen activator (rt-PA), before rt-PA administration based on their risk factors and some available laboratory results. Methods: We enrolled 118 ischemic stroke patients who were treated with standard dose of Alteplase in our hospital. Baseline characteristics, door-to-needle time (DTN), onset-to-treatment time (OTT), the National Institute Health Stroke Scale (NIHSS), systolic and diastolic blood pressure on admission, history of diabetes, hypertension, dyslipidemia, coronary artery disease (CAD), previous ischemic stroke, atrial fibrillation (AF), laboratory results were retrospectively collected. The modified Rankin Scale (mRS) was recorded after 3 months of admission and patients were divided into good (mRS 2) and poor (mRS>2) outcome groups. Chi-square test and t-test were used for categorical and continuous variables, respectively. Predictors for outcome after 3 months were studied by multivariable logistic regression. Results: Good outcome was seen in 60 (51%) patients and poor outcome was seen in 58 (49%) patients. Significant predictors for outcome at 3 months according to multivariable regression analysis were NIHSS score (odds ratio [OR], 0.61; 95% confidence interval [CI], 0.498-0.750; p<0.001), SBP (OR, 0.95; 95% CI, 0.925-0.991; P=0.01), AF (OR, 0.09; 95% CI, 0.013- 0.708; P=0.02), CAD (OR, 17.08; 95% CI, 0.013-0.708; p=0.003). Conclusion: Higher NIHSS score, higher SBP on admission, AF and history of CAD could be the independent predictors of outcome after IVT in acute ischemic stroke patients.
Strategy To Improve Printability of Renewable Resource-Based Engineering Plastic Tailored for FDM Applications

This work features the first-time use of poly(trimethylene terephthalate) (PTT), a biobased engineering thermoplastic, for fused deposition modeling (FDM) applications. Additives such as chain extenders (CEs) and impact modifiers are traditionally used to improve the processability of polymers for injection molding; as a proof of concept for their use in FDM, the same strategies were applied to PTT to improve its printability. The filament processing conditions and printing parameters were optimized to generate complete, warpage-free samples. The blends were characterized through physical, thermal, viscoelastic, and morphological analyses. In the optimal blend (90 wt % PTT, 10 wt % impact modifier, and 0.5 phr CE), the filament diameter was improved by ∼150%, the size of the spherulites significantly decreased to 5% of the ∼26 μm spherulite size found in neat PTT, and the melt flow index decreased to ∼4.7 g/10 min. From this blend, FDM samples with a high impact performance of ∼61 J/m were obtained, which are comparable to other conventional FDM thermoplastics. The ability to print complete and warpage-free samples from this blend suggests a new filament feedstock material for industrial and home-use FDM applications. This paper discusses methods to improve hard-to-print polymers and presents the improved printability of PTT as proof of these methods’ effectiveness.
First Reported Nosocomial Outbreak Of NDM-5-Producing Klebsiella pneumoniae In A Neonatal Unit In China

Purpose Carbapenem-resistant Klebsiella pneumoniae (CRKP) have emerged worldwide and also being a major threat to children and neonate. In this study, we describe a nosocomial outbreak of NDM-5-producing Klebsiella pneumoniae in neonatal unit of a teaching hospital in China from September 2015 to September 2016. Patients and methods We collected 12 carbapenem-resistant K. pneumoniae outbreak strains from 12 newborns and characterized these isolates for their antimicrobial susceptibility, clone relationships, and multi-locus sequence types using vitek-2 compact system, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Resistant genes were detected by using PCR and sequencing. Plasmid conjugation experiment was carried out to determine the transferability of carbapenem resistance. PCR-based replicon typing (PBRT), S1 nuclease-PFGE, and southern blotting were conducted for plasmid profiling. Results All 12 K. pneumoniae isolates were resistant to carbapenems and carried blaNDM-5, blaTEM-1 and blaSHV-11. Furthermore, PFGE analysis showed that NDM-5-producing K. pneumoniae were clonally related and MLST assigned them to sequence type 337. Conjugative assays showed that plasmids harboring blaNDM-5 gene were self-transmissible. Plasmid analysis suggested that all blaNDM-5 gene located on a ~45 kb IncX3 type plasmid. Conclusion To the best of our knowledge, this is the first report of a clone outbreak of blaNDM-5-carrying K. pneumoniae isolates from neonates. There is an urgent need for effective infection control measures to prevent blaNDM-5 variants from becoming epidemic in the neonates in the future.
Optimisation of Zn-Mn co-electrodeposition on aluminium utilising direct- and pulse current in alkaline and acidic electrolyte

Aluminium used in heat exchanger application is susceptible towards corrosion in aggressive environments. Protection of the aluminium surface is important for the metal to retain mechanical and chemical properties. Diffusion coating is considered a possibility for aluminium surface protection, where sacrificial metal is diffused into the metal surface and reducing the corrosion potential for the substrate. Zn has previously been utilised as sacrificial metal, but alloying elements in the diffusion coating are of interest due to their synergistic effect towards corrosion protection. Methods used for depositing coating on aluminium include thermal spray coatings. However problems with spray coating application, like overuse of feedstock, have lead to other possible methods being considered. One of these are electrodeposition. Deposition of coating with electrodeposition is easy to control with parameters like deposition time, temperature and current. In this thesis, electrodeposition of Zn-Mn was attempted using aluminium as substrate and utilising two different electrolytes; alkaline pyrophosphate and acidic sulphate electrolyte. Usage of two different current application, direct and pulse current, were examined. Characterisation of deposits through morphology, chemical composition and chemical depth profile was achieved by using SEM/EDS, GDOES and Raman spectroscopy methods for examination. Current efficiency of the baths for both current application were also reviewed, and the optimal setup was concluded for at the end. All samples in both electrolytes included oxygen in the final coating, with varying at. %. Most of the oxygen content was found, from EDS analysis, GDOES and Raman spectroscopy, to be from oxidation of metal deposited at the surface. Especially was this evident when Mn had deposited at surface, as the lowest content of oxygen found was that of the sample where Mn was mainly inside the coating. Inclusion of the less noble metal, Mn, in the coating was favoured for all samples when applying larger cathodic current densities. This result was effected from different hydrogen evolution intensity regarding deposition. Pulse current application favoured more compact and uniform deposit distribution for lower current densities, and less uniformity and more clustered morphology for larger current densities. Co-deposition of Zn-Mn was favoured using PC setup, and more specifically at the edges of the samples where the cathodic current density was largest. Current efficiency was overall larger for alkaline pyrophosphate than the acidic sulphate bath, and is the electrolyte displaying best properties.
Impact of the teaching method of the rub-in technique for learning hygienic hand disinfection in medical studies: a comparative effectiveness analysis of two techniques

Objective: Hygienic hand disinfection is of major importance regarding nosocomial infections and antibiotic resistance. The six-step technique is the most commonly taught method, but its superiority has not been empirically demonstrated. This study compares two hand disinfection techniques with regard to their total distribution of the disinfectant. Methods: In this comparative effectiveness analysis, medical students were randomized into two groups. Group 1 was instructed in the 6-step technique, group 2 was referred to a self-responsible application. Learning success was measured using fluorescent disinfectant and black light photographs at three time points (directly, few days later, 5–12 weeks later). Photographs were evaluated quantitatively. Results: 198 students were included in the study (Group 1: 6-step technique; n=103, Group 2: self-responsible disinfection; n=95). 186 were followed up at the second measurement, 182 at the third measurement. Directly after training, there were no significant differences between the two groups. At the second measurement, Group 2 outperformed Group 1 for total, dorsal, and palmar areas (p<0.001, p=0.002, p<0.001). At the third measurement, Group 2 was significantly better (p=0.019) for palmar-sided hands. In Group 1, areas of disinfected skin deteriorated significantly between measurement 1 and 2 (p=0.019) and measurement 2 and 3 (p<0.001). Group 2 did not deteriorate between measurement 1 and 2 (p=0.269) but between measurement 2 and 3 (p<0.001). Conclusions: Compared to the established six-step technique, a self-responsible application method results in measurably better distribution of the hand disinfectant.
Structural transformation of graphite by passage of electric current

Abstract The passage of an electric current through graphite, or few-layer graphene, at high temperatures can produce dramatic structural transformations. These involve the formation of carbon structures with highly irregular morphologies, displaying many unusual features, including nanotube–graphene junctions. A number of studies of the transformed carbon have been carried out, but many aspects of its structure and formation mechanism are still not understood. This paper summarises the studies that have been made on this phenomenon, drawing mainly on the author’s own work. It is shown, using high-tilt transmission electron microscopy imaging, that the transformed carbon is largely flat, and not three-dimensional as suggested in previous papers. The possible mechanism of the transformations is discussed.
Biodegradation of malachite green by a newly isolated Bacillus vietnamensis sp. MSB17 from continental slope of the Eastern Arabian Sea: Enzyme analysis, degradation pathway and toxicity studies

Abstract A marine bacterial isolate, Bacillus vietnamensis sp. MSB17 from the sediments collected from the continental slope of the eastern Arabian Sea exhibited good Malachite Green (MG) decolorization ability in mineral medium supplemented with glucose and yeast extract under static conditions. The complete decolorization of dye (50 mg/L) was attained within 4 h of incubation. The activities of enzymes such as tyrosinase (0.62 IU/ml), laccase (12.02 U/ml), and manganese peroxidase (6.23 U/ml) were observed during dye degradation. UV-VIS, FT-IR, and GC-MS analysis of dye samples extracted with ethyl acetate confirmed the biodegradation of MG into methanone, [4-(dimethylamino) phenyl] phenyl- and 2, 6-bis (1, 1-dimethylethyl) phenol. The metabolites obtained from B. vietnamensis sp. MSB17 mediated MG degradation did not exhibit any microbial toxicity and phytotoxicity. The present study indicated the efficiency of Bacillus vietnamensis sp. MSB17 to effectively degrade MG, revealing its potential to be further utilized for bioremediation purposes.
PREVENTION AND MANAGEMENT OF HIV AIDS

HIV (human immunodeficiency virus) is the virus that causes AIDS. This virus may be passed from one person to another when infected blood, semen, or vaginal secretions come in contact with an uninfected person's broken skin or mucous membrane. In addition, infected pregnant women can pass HIV to their baby during pregnancy or delivery, as well as through breast-feeding. HIV symptoms associate with confusion and forget fullness nausea, stomach cramps diarrhea, vomiting vision loss Rapid weight loss Dry cough . the center for control of diseases estmates 70 % of the annual infections trans mitted by persons who are un aware of infection. The immune dysfunction progress to aids development of infections from months to years. The initial findings include leucopenia ulcers lymphadenopathy hepatosplenomegaly neurological exam. The HIV is diagnosis by serology test Elisa test western Blot, PCR test NAT test. The anti viral drugs such as zidovudine didanosine nelfinavir lamivudine Comvivir abvacavir used to treat the disease. The combination of doses and immunization therapy is used to prevent the aids. The prevention of the disease is through media. Establishing the counseling centers rehabilitation centers in the country.
Screening and Comparative Characterization of Microorganisms from Iranian Soil Samples Showing ω-Transaminase Activity toward a Plethora of Substrates

In this study, soil microorganisms from Iran were screened for ω-transaminase (ω-TA) activity based on growth on minimal media containing (rac)-α-methylbenzylamine (rac-α-MBA) as a sole nitrogen source. Then, for the selection of strains with high enzyme activity, a colorimetric o-xylylendiamine assay was conducted. The most promising strains were identified by 16S rDNA sequencing. Five microorganisms showing high ω-TA activity were subjected to determine optimal conditions for ω-TA activity, including pH, temperature, co-solvent, and the specificity of the ω-TA toward different amine donors and acceptors. Among the five screened microorganisms, Bacillus halotolerans turned out to be the most promising strain: Its cell-free extract showed a highly versatile amino donor spectrum toward aliphatic, aromatic chiral amines and a broad range of pH activity. Transaminase activity also exhibited excellent solvent tolerance, with maximum turnover in the presence of 30% (v/v) DMSO.
Professional practices and recommendations Physical exercise supervised or not by a physiotherapist in the treatment of lower-limb osteoarthritis. Elaboration of French clinical practice guidelines

Objectives. – To develop clinical practice guidelines concerning supervised or unsupervised kinesiotherapy for treating lower-limb osteoarthritis (OA). Method. – The SOFMER (French Physical Medicine and Rehabilitation Society) methodology, associating systematic literature review, collection of everyday clinical practice, and external review by a multidisciplinary expert panel, was used to develop guidelines. Results–conclusion. – Physical exercise is a part of the treatment of lower-limb OA. An educational pre-program is recommended to inform the patient about the ease and effectiveness of the physical exercise. Use of the SOFMER methodology led to recommending a program of initial physical exercise supervised by a physiotherapist, then an unsupervised program at home with compliance. The type, intensity, and frequency of the exercises must be adapted to each patient. Complementary randomized controlled studies are necessary to characterize the best exercises and their intensity and frequency. The OA location and gravity, functional need, and characteristics of patients will be useful in future studies. © 2007 Elsevier Masson SAS. All rights reserved.
Proximate and phenolic composition of selected native Australian food plants

Edible portions of Leucopogon parviflorus, Arthropodium strictum, Carpobrotus rossii, Rhagodia candolleana, Typha orientalis, Correa alba, Dianella revoluta and Acacia longifolia were collected in 2017 and 2019 from Warrnambool, Victoria Australia, and studied for phenolics and proximate composition. The plant species recorded consistent antioxidant activity with no nutritional alterations. Except for A. longifolia, total phenolic contents (TPC) were higher in 2019 than 2017 (P < 0.05). All the plant species contained Ca, Mg, Na and K. Except for betanin, isolated from R. candolleana (700 mg L⁻¹) and C. rossii (244 mg L⁻¹), higher quantities of polyphenols were extractable in methanol than water (P < 0.05). The plant fatty acid composition was predominantly palmitic, oleic and linoleic acids with A. longifolia containing 44% linoleic acid. While the plant nutrients and fatty acids were fairly stable, TPCs, some of the minerals and polyphenols varied between the years.
Impact of health information technology on delivery and quality of patient care

by AMANDA JANE HESSELS A Dissertation submitted to the Graduate School-Newark Rutgers, the State University of New Jersey in partial fulfillment of the requirements for the degree of Doctor of Philosophy Graduate Program in Nursing written under the direction of Linda Flynn, PhD, RN, FAAN and approved by _____________________________________ _____________________________________ _____________________________________ _____________________________________ _____________________________________