Miracle-dz/UniFAR-FTUs huggingface dataset:
Sequential Cell-Processing System by Integrating Hydrodynamic Purification and Dielectrophoretic Trapping for Analyses of Suspended Cancer Cells

Microfluidic devices employing dielectrophoresis (DEP) have been widely studied and applied in the manipulation and analysis of single cells. However, several pre-processing steps, such as the preparation of purified target samples and buffer exchanges, are necessary to utilize DEP forces for suspended cell samples. In this paper, a sequential cell-processing device, which is composed of pre-processing modules that employ deterministic lateral displacement (DLD) and a single-cell trapping device employing an electroactive microwell array (EMA), is proposed to perform the medium exchange followed by arraying single cells sequentially using DEP. Two original microfluidic devices were efficiently integrated by using the interconnecting substrate containing rubber gaskets that tightly connect the inlet and outlet of each device. Prostate cancer cells (PC3) suspended in phosphate-buffered saline buffer mixed with microbeads were separated and then resuspended into the DEP buffer in the integrated system. Thereafter, purified PC3 cells were trapped in a microwell array by using the positive DEP force. The achieved separation and trapping efficiencies exceeded 94% and 93%, respectively, when using the integrated processing system. This study demonstrates an integrated microfluidic device by processing suspended cell samples, without the requirement of complex preparation steps.
Liver tissue classification of en face images by fractal dimension‐based support vector machine

Full‐field optical coherence tomography (FF‐OCT) has been reported with its label‐free subcellular imaging performance. To realize quantitive cancer detection, the support vector machine model of classifying normal and cancerous human liver tissue is proposed with en face tomographic images. Twenty samples (10 normal and 10 cancerous) were operated from humans and composed of 285 en face tomographic images. Six histogram features and one proposed fractal dimension parameter that reveal the refractive index inhomogeneities of tissue were extracted and made up the training set. The other different 16 samples (8 normal and 8 cancerous) were imaged (190 images) and employed as the test set with the same features. First, a subcellular‐resolution tomographic image library for four histopathological areas in liver tissue was established. Second, the area under the receiver operating characteristics of 0.9378, 0.9858, 0.9391, 0.9517 for prediction of the cancerous hepatic cell, central vein, fibrosis, and portal vein were measured with the test set. The results indicate that the proposed classifier from FF‐OCT images shows promise as a label‐free assessment of quantified tumor detection, suggesting the fractal dimension‐based classifier could aid clinicians in detecting tumor boundaries for resection in surgery in the future.
A STUDY OF DEPRESSION AMONG MEDICAL STUDENTS OF PRIVATE MEDICAL COLLEGE IN SOUTH INDIA

CONTEXT: Medical Education in Private Medical Colleges is a great contributor to stress among the medical students & possibly even in developing syndromic depression among medical students which is an area of concern worldwide. The objective of this study is to assess the prevalence of depressive symptoms and its associate factors among medical students. MATERIALS AND METHODS: A cross sectional survey was conducted among 400 medical students from first to fourth year in Private Medical College of South India. Beck Depression Inventory-II was used to assess the level of depression with score of 10 or higher considered depressive. Association between depression and sex, year of study, medium of teaching in 10+2, social factors like alcohol & other substance abuse, family history of depression & family problems, hostel-stay etc. were analyzed by EPI info version 7. RESULTS: A total of 400 medical students participated in the study and the overall prevalence was found to be 64%. The prevalence of depression was higher (79%) among newly entered students (1st year) as compared to 2nd, 3rd and 4th year which was 60%, 57% and 53%, respectively. It was statistically significant (X 2 =38.54, p=0.001252 with Yate's correction). Students who were facing language problem in their MBBS course, because English was not the medium of teaching in their 10+2, reported symptoms suggestive of depression (X 2 =9.2091, p=0.0024). On the other hand students who undertook regular physical exercise were likely to suffer less depression (statistically significant, X2=34, p=0.000). Students taking alcohol (X2=8.315, p=0.00392) and with other substance abuse (X2=6.277, p=0.01233) reported more symptoms suggestive of depression. There was no statistically significant difference in the prevalence of depression among students with family history and staying in hostel. CONCLUSIONS: Prevalence of depression is quite high in students of Private Medical College as revealed by this study. It is recommended that a screening may be carried out based on BDI-II scale at the end of first year and group counseling facilities by the Department of Psychiatry within the medical college should be made available to affected medical students to lead a healthier life.
Fibre‐specific regulation of lignin biosynthesis improves biomass quality in Populus

Summary Lignin is a major component of cell wall biomass and decisively affects biomass utilisation. Engineering of lignin biosynthesis is extensively studied, while lignin modification often causes growth defects. We developed a strategy for cell‐type‐specific modification of lignin to achieve improvements in cell wall property without growth penalty. We targeted a lignin‐related transcription factor, LTF1, for modification of lignin biosynthesis. LTF1 can be engineered to a nonphosphorylation form which is introduced into Populus under the control of either a vessel‐specific or fibre‐specific promoter. The transgenics with lignin suppression in vessels showed severe dwarfism and thin‐walled vessels, while the transgenics with lignin suppression in fibres displayed vigorous growth with normal vessels under phytotron, glasshouse and field conditions. In‐depth lignin structural analyses revealed that such cell‐type‐specific downregulation of lignin biosynthesis led to the alteration of overall lignin composition in xylem tissues reflecting the population of distinctive lignin polymers produced in vessel and fibre cells. This study demonstrates that fibre‐specific suppression of lignin biosynthesis resulted in the improvement of wood biomass quality and saccharification efficiency and presents an effective strategy to precisely regulate lignin biosynthesis with desired growth performance.
Smoking Among U.S. Service Members Following Transition From Military to Veteran Status

Smoking rates among U.S. Service members and veterans have been consistently higher than in civilian populations. While much has been published about tobacco use in both military and veteran populations, smoking patterns during transition from military to veteran status remains unclear. We studied military members participating in the Millennium Cohort Study, who separated from their respective Services between baseline and first follow-up survey (N = 5,510). Two generalized estimating equation models were used to examine any association between smoking status and time to military separation (days between baseline survey and separation), as well as smoking during transition and reason for separation. Reason for separation was categorized into three groups: (1) not meeting military standards or judicial-related reasons, (2) drugs-/alcohol-related misconduct, and (3) other types of separation including retirement, pregnancy, and so on. Statistical models accounted for baseline smoking and demographic/military/health behavioral/mental health characteristics. Overall, we observed a decline in smoking prevalence over time (19.5%, 16.7%, 15.2%, and 12.6%, respectively). However, we found a 22% increase in the odds of smoking among those who stayed in the military between 3 months and 1 year, compared to those who stayed 2+ years. Additionally, participants separating for standard/judicial reason(s) showed 69% increased smoking compared to those with other reasons for separation. The time period immediately prior to Service separation and certain types of separation were associated with increased odds of smoking. Thus, smoking cessation interventions should target Service members during this transition period to potentially reduce smoking prevalence after separation.
Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells

Polyethyleneimine, poly-L-lysine, chitosan and some others cationic polymers have been thoroughly studied as nucleic acid delivery systems in gene therapy. However, the drug release from these systems proceeds at a very low rate due to extremely high binding between a carrier and gene material. To reduce these interactions and to enhance drug release, we developed a set of amphiphilic polypeptides containing positively and negatively charged amino acids as well as a hydrophobic one. The copolymers obtained were characterized by size-exclusion chromatography, static light scattering, HPLC amino acid analysis and 1HNMR spectroscopy. All copolymers formed particles due to a self-assembly in aqueous media. Depending on polypeptide composition, the formation of particles with hydrodynamic diameters from 180 to 900 nm was observed. Stability of polymer particles, loading and release efficiency were carefully studied. Cellular uptake of the particles was efficient and their cytotoxicity was negligible. The application of polymer carriers, containing siRNA, to vascular endothelial growth factor (VEGF-A165) silencing of ARPE-19 cells was successful. The gene silencing was confirmed by suppression of both messenger RNA and protein expression.
Porcine Epidemic Diarrhea Altered Colonic Microbiota Communities in Suckling Piglets

Porcine epidemic diarrhea (PED) is a major gastrointestinal disease afflicting suckling pigs that causes huge industrial economic losses. In this study, we investigated microbiota from the colonic mucosa and content in healthy and PED piglets. High-throughput 16S rRNA gene sequencing was performed to identify inter-group differences. Firmicutes, Fusobacteria, Proteobacteria, and Bacteroidetes were the top four affected phyla. The proportion of Proteobacteria was higher in infected than in healthy piglets, and the opposite was observed for Bacteroidetes (more than four-fold higher in the healthy group). In the infected group, Fusobacterium accounted for 36.56% and 21.61% in the colonic mucosa and contents, respectively, while in the healthy group, they comprised 22.53% and 12.67%, respectively. The percentage of Lactobacillus in healthy colons (15.63%) was considerably higher than that in the disease group (<10%). In both the colonic mucosa and contents, functional enrichment differed significantly between healthy and diseased groups. Overall, infection with the PED virus increased the proportion of harmful bacteria and decreased the proportion of beneficial bacteria in the colons of piglets. Targeting intestinal microbiota could be a promising method for PED prevention, thus opening new avenues for future research.
In Situ Proteolysis Condition-Induced Crystallization of the XcpVWX Complex in Different Lattices

Although prevalent in the determination of protein structures; crystallography always has the bottleneck of obtaining high-quality protein crystals for characterizing a wide range of proteins; especially large protein complexes. Stable fragments or domains of proteins are more readily to crystallize; which prompts the use of in situ proteolysis to remove flexible or unstable structures for improving crystallization and crystal quality. In this work; we investigated the effects of in situ proteolysis by chymotrypsin on the crystallization of the XcpVWX complex from the Type II secretion system of Pseudomonas aeruginosa. Different proteolysis conditions were found to result in two distinct lattices in the same crystallization solution. With a shorter chymotrypsin digestion at a lower concentration; the crystals exhibited a P3 hexagonal lattice that accommodates three complex molecules in one asymmetric unit. By contrast; a longer digestion with chymotrypsin of a 10-fold higher concentration facilitated the formation of a compact P212121 orthorhombic lattice with only one complex molecule in each asymmetric unit. The molecules in the hexagonal lattice have shown high atomic displacement parameter values compared with the ones in the orthorhombic lattice. Taken together; our results clearly demonstrate that different proteolysis conditions can result in the generation of distinct lattices in the same crystallization solution; which can be exploited in order to obtain different crystal forms of a better quality
Lnc‐MEG3 acts as a potential biomarker for predicting increased disease risk, systemic inflammation, disease severity, and poor prognosis of sepsis via interacting with miR‐21

This study aimed to investigate the correlations of long non‐coding RNA maternally expressed gene 3 (lnc‐MEG3), microRNA (miR)‐21, and lnc‐MEG3/miR‐21 axis with disease risk, inflammation, disease severity, and 28‐day mortality of sepsis.
The astrocyte network in the ventral nerve cord neuropil of the Drosophila third‐instar larva

Understanding neuronal function at the local and circuit level requires understanding astrocyte function. We have provided a detailed analysis of astrocyte morphology and territory in the Drosophila third‐instar ventral nerve cord where there already exists considerable understanding of the neuronal network. Astrocyte shape varies more than previously reported; many have bilaterally symmetrical partners, many have a high percentage of their arborization in adjacent segments, and many have branches that follow structural features. Taken together, our data are consistent with, but not fully explained by, a model of a developmental growth process dominated by competitive or repulsive interactions between astrocytes. Our data suggest that the model should also include cell‐autonomous aspects, as well as the use of structural features for growth. Variation in location of arborization territory for identified astrocytes was great enough that a standardized scheme of neuropil division among the six astrocytes that populate each hemi‐segment is not possible at the third instar. The arborizations of the astrocytes can extend across neuronal functional domains. The ventral astrocyte in particular, whose territory can extend well into the proprioceptive region of the neuropil, has no obvious branching pattern that correlates with domains of particular sensory modalities, suggesting that the astrocyte would respond to neuronal activity in any of the sensory modalities, perhaps integrating across them. This study sets the stage for future studies that will generate a robust, functionally oriented connectome that includes both partners in neuronal circuits—the neurons and the glial cells, providing the foundation necessary for studies to elucidate neuron–glia interactions in this neuropil.
Experiences of Tobacco Use among Chinese Individuals with Schizophrenia in Community-Based Residential Settings: A Qualitative Study

Although there is a high prevalence of smoking among individuals with schizophrenia, no previous attempt has been made to explore experiences of tobacco use and cessation within a Chinese sample of this population. A qualitative descriptive study was conducted to explore through the use of individual and semi-structured interviews the experiences of tobacco use and quitting in a sample Chinese population with schizophrenia. Twenty-three eligible participants with schizophrenia who currently smoke were recruited from three community residential mental health service settings. Four main themes representing the experiences of the participants were uncovered in this study: (1) smoking and illness; (2) environment and culture; (3) reasons for smoking; and (4) beliefs about cessation methods. The findings indicated that the participants considered the disease to be far more harmful than smoking, and perceived many benefits to smoking. Although some thought of quitting at times, most gave up the idea or failed to quit due to internal factors such as disease-related suffering, the use of an avoidance coping strategy, and a lack of cessation support. The most notable finding concerns the use of avoidance coping by the participants, who relied on smoking as a way to avoid the suffering brought about by the disease.
Evidence of gut enteropathy and factors associated with undernutrition among slum-dwelling adults in Bangladesh

ABSTRACT Background Adult undernutrition (BMI <18.5 kg/m2) is responsible for immune deficits, increased risk of disease burden, and higher rates of mortality. The prevalence of adult undernutrition in Bangladesh is substantial, but there have been few studies on the etiology of this condition for the inhabitants of urban slums. Objective The aim of this study was to identify the factors associated with undernutrition among slum-dwelling adults in Bangladesh. Methods A case-control study was conducted in the Bauniabadh area of Dhaka, Bangladesh. 270 adult participants (135 cases with a BMI <18.5 and 135 controls with a BMI between 18.5 and 24.9) aged 18–45 y were enrolled between October 2018 and January 2019. Sociodemographic variables, dietary diversity, micronutrient deficiencies, psychological symptoms, infection, and biomarkers of gut health were assessed to identify the factors associated with undernutrition using multivariable logistic regression analysis. Results A higher number of siblings [adjusted odds ratio (aOR): 1.39; 95% CI: 1.11, 1.77], increased self-reporting questionnaire-20 score (an instrument to screen mental health disorders and detect psychological symptoms) (aOR: 1.12; 95% CI: 1.04, 1.23), elevated fecal concentration of α-1 antitrypsin (aOR: 4.82; 95% CI: 1.01, 25.29), and anemia (aOR: 3.63; 95% CI: 1.62, 8.58) were positively associated with undernutrition in adults. Age (aOR: 0.90; 95% CI: 0.84, 0.96), dietary diversity score (aOR: 0.75; 95% CI: 0.56, 0.99), C-reactive protein (aOR: 0.82; 95% CI: 0.73, 0.92), Helicobacter pylori infection (aOR: 0.11; 95% CI: 0.05, 0.23), and always washing hands before eating or preparing foods (aOR: 0.33; 95% CI: 0.12, 0.87) were associated with reduced odds of undernutrition among the study population. Conclusions Our results indicate that undernutrition in slum-dwelling adults in Bangladesh is associated with numerous physiological and sociodemographic factors, including evidence of gastrointestinal inflammation and altered intestinal permeability.
Ruminal Fermentation, Growth Rate and Methane Production in Sheep Fed Diets Including White Clover, Soybean Meal or Porphyra sp.

Simple Summary In ruminant feeding, the use of diets containing seaweeds could be a valuable alternative to conventional diets. The objective of this work was to investigate the ruminal fermentation, growth rate and methane production in sheep fed a diet including Porphyra sp. compared with diets including clover silage or soybean meal. Including Porphyra sp. had little impact on ruminal fermentation and methane production both in vitro and in vivo. Lambs fed Porphyra sp. had a similar growth rate to those fed a diet including soybean meal, confirming previous in vitro and in situ observations on the high-quality protein of Porphyra sp. in ruminant feed. Abstract The aim of the present work was to investigate the potential of Porphyra sp. as an alternative source of protein to soybean meal in diets for sheep. Our experimental treatments included a control diet (CON) based on grass silage and crushed oats and three diets containing protein supplements, clover silage (CLO), soybean meal (SOY) or Porphyra sp. (POR) to increase dietary crude protein concentrations. We studied its effects on rumen fermentation, growth rate and methane emissions. Ruminal fermentation characteristics, kinetics of gas production and methane production were studied in vitro by using batch cultures inoculated with rumen inoculum from sheep. There were no differences among diets in total volatile fatty acids (VFA) production or in the VFA profile in vitro. Across treatments, we measured no differences in methane production either in vitro or in vivo, and we saw no noticeable antimethanogenic effect of Porphyra sp. The present in vivo trial with lambs showed no differences in average daily weight gain when fed diets including Porphyra sp. or soybean meal diets (250 and 254 g/d, respectively). We conclude that Porphyra sp. has a protein value similar to high-quality protein sources like soybean meal.
Optogenetic‐induced sympathetic neuromodulation of brown adipose tissue thermogenesis

The brown adipose tissue (BAT) is a thermogenic organ that plays a major role in energy balance, obesity, and diabetes due to the potent glucose and lipid clearance that fuels its thermogenesis, which is largely mediated via sympathetic nervous system activation. However, thus far there has been little experimental validation of the hypothesis that selective neuromodulation of the sympathetic nerves innervating the BAT is sufficient to elicit thermogenesis in mice. We generated mice expressing blue light‐activated channelrhodopsin‐2 (ChR2) in the sympathetic nerves innervating the BAT using two different strategies: injecting the BAT of C57Bl/6J mice with AAV6‐hSyn‐ChR2 (H134R)‐EYFP; crossbreeding tyrosine hydroxylase‐Cre mice with floxed‐stop ChR2‐EYFP mice. The nerves in the BAT expressing ChR2 were selectively stimulated with a blue LED light positioned underneath the fat pad of anesthetized mice, while the BAT and core temperatures were simultaneously recorded. Using immunohistochemistry we confirmed the selective expression of EYFP in TH positive nerves fibers. In addition, local optogenetic stimulation of the sympathetic nerves induced significant increase in the BAT temperature followed by an increase in core temperature in mice expressing ChR2, but not in the respective controls. The BAT activation was also paralleled by increased levels of pre‐UCP1 transcript. Our results demonstrate that local optogenetic stimulation of the sympathetic nerves is sufficient to elicit BAT and core thermogenesis, thus suggesting that peripheral neuromodulation has the potential to be exploited as an alternative to pharmacotherapies to elicit organ activation and thus ameliorate type 2 diabetes and/or obesity.