Miracle-dz/UniFAR-FTUs huggingface dataset:
Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice.

AIM To study whether matrix metalloproteinase-9 (MMP-9) is a key factor in epithelial damage in the dextran sodium sulphate (DSS) model of colitis in mice. METHODS MMP-9-deficient and wild-type (wt) mice were given 5% DSS in drinking water for 5 d followed by recovery up to 7 d. On d 5 and 12 after induction of colitis, gelatinases, MMP-2 and MMP-9, were measured in homogenates of colonic tissue by zymography and Western blot, whereas tissue inhibitor of metalloproteinases (TIMPs) were measured by reverse zymography. The gelatinolytic activity was also determined in supernatants of polymorphonuclear leukocytes (PMN) isolated from mice blood. Moreover, intestinal epithelial cells were stimulated with TNF-alpha to study whether these cells were able to produce MMPs. Finally, colonic mucosal lesions were measured by microscopic examination. RESULTS On d 5 of colitis, the activity of MMP-9 was increased in homogenates of colonic tissues (0.24+/-0.1 vs 21.3+/-6.4, P<0.05) and PMN from peripheral blood in wt (0.5+/-0.1 vs 10.4+/-0.7, P<0.05), but not in MMP-9-deficient animals. The MMP-9 activity was also up-regulated by TNF-alpha in epithelial intestinal cells (2.5+/-0.5 vs 14.7+/-3.0, P<0.05). Although colitis also led to increase of TIMP-1 activity, the MMP-9/TIMP-1 balance remained elevated. Finally, in the MMP-9-deficient colitic mice both the extent and severity of intestinal epithelial injury were significantly attenuated when compared with wt mice. CONCLUSION We conclude that DSS induced colitis is markedly attenuated in animals lacking MMP-9. This suggests that intestinal injury induced by DSS is modulated by MMP-9 and that inhibition of this gelatinase may reduce inflammation.
Hardware and software architectures for computationally efficient three-dimensional ultrasonic data compression

Ultrasonic industrial and medical imaging applications involve acquisition of large amount of volumetric data in real time. Therefore, data storage becomes critical in many current day applications which utilise ultrasound technology. Compressing the acquired data allows possessing minimal storage and also helps to rapidly transmit information to remote locations for expert analysis. The objective of this study is to design computationally efficient architectures for implementing discrete wavelet transform-based ultrasonic three-dimensional (3D) data compression algorithm on a reconfigurable ultrasonic system-on-chip (SoC) hardware platform. In this study, hardware and software architectures of the 3D ultrasonic compression algorithm are realised using Xilinx Zynq all programmable SoC. This study demonstrates that, compressing 33 MB of experimental ultrasonic 3D data into 0.42 MB (98.7% compression) requires only 84 ms for hardware architecture, and 1 min for software architecture, making both designs highly suitable for real-time ultrasonic imaging applications. Furthermore, the 3D compression is implemented by using Open Computing Language (OpenCL) targeted on Nvidia GT 750M graphical processing unit. OpenCL implementation of ultrasonic 3D compression algorithm completes the execution in <1 sec. This approach provides improved computational performance as that of hardware architecture, and comparable flexibility as that of software implementation.
Microcatheter contrast injection in stent retriever neurothrombectomy is safe and useful: insights from SWIFT PRIME

Microcatheter contrast injection (MCI) prior to stent retriever deployment for the treatment of acute ischemic stroke may be useful for evaluation of distal anatomy and flow patterns beyond the occlusion. However, prior data from intra-arterialthrombolysis suggested that MCI increases the risk of intracranial hemorrhage (ICH). The safety and utility of MCI has not been investigated in the setting of thrombectomy. Methods We analyzed the Solitaire With the Intention For Thrombectomy as Primary Endovascular Treatment (SWIFT PRIME) trial to correlate pre-intervention MCI flow with collateral flow, and to investigate its impact on ICH and clinical outcome after thrombectomy with the Solitaire device. Results MCI was noted in 52% (n=51) of patients with a prevalence for the M2 location of 71% (n=36). Dichotomized correlation demonstrated a strong inverse relationship for partial collaterals with good MCI flow (p=0.004; OR 8.25). None of the MCI variables (presence, number, or grades) correlated with ICH and clinical outcome. The most significant predictors of non-disabled outcome were higher Alberta Stroke Program early CT Score (ASPECTS) (OR 1.61; p=0.0361) and younger age (OR 0.922; p = 0.0109). Higher ASPECTS was also a strong predictor of lower ICH risk (OR 0.501, p=0.0078). Conclusions Collateral flow inversely correlated with MCI flow in the endovascular arm of the SWIFT PRIME trial. This finding warrants further validation in larger cohorts as MCI may be influenced by individual operator’s technique and choice of syringe size. Evaluation of flow and distal anatomy with MCI prior to stent retriever deployment is safe with no evidence of an impact on ICH or clinical outcome. Clinical trial registration NCT01657461: Post- results
First Laboratory Culture of Phortica variegata (Diptera, Steganinae), a Vector of Thelazia callipaeda

[Extract] The family Drosophilidae includes ~4,500 species in the two subfamilies Drosophilinae and Steganinae that are generally known as fruit flies or pomace flies because of their habit of feeding and growing on fermenting fruit. Within the Drosophilinae, Drosophila species are among the best characterized model organisms in genetic and genomic research. Conversely, knowledge of species ranked within the Steganinae is limited to a few morphological, taxonomic, and systematic investigations of selected members and information of the biology and ecology of most species is scarce. Over the past decade, fruit flies of the genus Phortica have been the subject of intense investigations aimed at identifying the arthropod vector and intermediate host of Thelazia spp. eyeworms (Otranto et al. 2003, 2005, 2006a, 2006b, Dorchies et al. 2007, Roggero et al. 2010). In particular, while Phortica okadai (Maca, 1977), Phortica magna (Okada 1968), and Amiota nagatai Okada, 1971 are vectors of Thelazia spp. in Asian countries (Kamakura et al. 1998), Phortica variegata (Fallen 1823) has been demonstrated, under both experimental (Otranto et al. 2005) and natural conditions (Otranto et al. 2006b), to harbor larvae of Thelazia callipaeda Railliet and Henry 1910 "eyeworm" nematodes in Europe. Phortica spp. differ remarkably from other fruit flies in that they are known to feed not only on decaying fruit and slime fluxes, but also on lachrymal secretions of animals and humans (Bachli et al. 2004). When feeding on the conjunctiva of vertebrates infested with T. callipaeda, P. variegata ingests the first-stage larvae released by adult female nematodes; within the body cavities of the arthropod vector, these larvae develop to second-stage larvae and, subsequently, to infective third-stage larvae which reach the proboscis of the fly and are invade the conjunctival sac of a new, receptive host (reviewed by Otranto et al. 2003). While both adult female and male P. variegata have been shown to harbour larvae of T. callipaeda under experimental settings (Otranto et al. 2005), only the latter gender has been demonstrated to act as a vector of this parasitic nematode under natural conditions (Otranto et al. 2006b, reviewed by Otranto et al. 2008a). The biological bases of this host-parasite relationship are still unclear.
Detection of mental fatigue using an active BCI inspired signal processing chain

Abstract This paper presents a novel electro-encephalography (EEG) signal processing chain designed to classify two levels of mental fatigue that appears after having spent a long time on a tedious task. The decrease in vigilance associated with mental fatigue makes it a dangerous state for operators in charge of complex systems. The processing chain, inspired from active brain computer interface computing, is implemented as follows: the EEG signal is initially filtered in a given frequency band and 15 electrodes out of 32 are then selected using a method based on Riemannian geometry. Next, a spatial filtering step is carried out using 6 common spatial pattern (CSP) filters. Lastly, a binary classification is performed using Fisher's linear discriminant analysis (FLDA). The features used are the log variance of the 6 CSP filtered signals. The results obtained on 20 healthy volunteers are excellent with 100% of accuracy when the beta band is used. These performances drop to 84% and 68% when the same data are processed with a traditional signal processing chain where fatigue is classified by means of a FLDA classifier fed by the averaged power, or relative power, in the beta band extracted from 15 selected electrodes.
Breast Cancer Surveillance Consortium: a national mammography screening and outcomes database.

M ammography is the primary method of detecting early stage breast cancer and has been shown in randomized clinical trials to reduce breast cancer mortality, especially among women 50 years old and older [1-5]. Authorities in cancer screening have bong recognized that the level of efficacy of screening demonstrated in randomized clinical trials may not pertain to community practice for several reasons [6]. These reasons include possible differences in the population groups receiving screening, lower accuracy of screening mammography in the community, and lower compliance with diagnostic follow-up and treatment in community practice, which may result in more adverse outcomes. Screening effectiveness in community practice today could exceed that estimated in trials because the technical and interpretative quality of mammography has improved since the trials were performed. Furthermore, clinical trial efficacy has been estimated on the basis of assignment to receive screening; to the extent that women assigned to screening were not screened or that women in the control groups were screened, efficacy in trials may have been underestimated. To optimally evaluate the performance of mammography in a community setting, the screening prevalence and patterns and the associated sensitivity, specificity, and predictive value of mammography in community screening programs should be determined by linkage with cancer outcomes [7, 8]. A program of monitoring should also provide data on specific populations, such as rural and minority subgroups, that are traditionally underserved by screening programs and that may have different breast cancer mortality rates [9]. Before the Mammography Quality Standards Act (MQSA) of 1992, most mammography facilities in the United States did not maintain records that could provide reliable and comprehensive data to evaluate the performance of screening mammography [10]. The concept of a medical audit of outcomes data had been proposed [ 1 1 ] but has not been routinely practiced in the community. The interim regulations of the MQSA mandated maintaining mammography data and performing a medical outcomes audit [12]. In practical terms, the medical audit requirement of the MQSA was limited to an analysis of patients with tests interpreted as “suspicious abnormality” or “highly suggestive for malignancy,” which permits evaluation of the positive predictive value of such interpretations. However, the MQSA does not require linkage to populationbased cancer registry data or another source of pathology data, without which it is impossible to accurately assess the outcomes of patients with mammograms interpreted as having normal findings. To understand the full effect of
Trajectory of change in pain, depression, and physical functioning after physical activity adoption in fibromyalgia

Fibromyalgia is associated with widespread pain, depression, and declines in physical functioning. The purpose of this study was to examine the trajectory of these symptoms over time related to physical activity adoption and maintenance via motivational interviewing versus education, to increase physical activity. There were no treatment group differences; we divided the sample (n = 184) based on changes in physical activity. Repeated measures analyses demonstrated differential patterns in depression, pain, and physical functioning at 24 and 36 weeks. Findings suggest increased physical activity may serve as a multiple-target intervention that provides moderate to large, long-lasting benefits for individuals with fibromyalgia.
Estrogen in the prevention of atherosclerosis. A randomized, double-blind, placebo-controlled trial.

BACKGROUND Although observational studies suggest that estrogen replacement therapy (ERT) reduces cardiovascular morbidity and mortality in postmenopausal women, use of unopposed ERT for prevention of coronary heart disease in healthy postmenopausal women remains untested. OBJECTIVE To determine the effects of unopposed ERT on the progression of subclinical atherosclerosis in healthy postmenopausal women without preexisting cardiovascular disease. DESIGN Randomized, double-blind, placebo-controlled trial. SETTING University-based clinic. PATIENTS 222 postmenopausal women 45 years of age or older without preexisting cardiovascular disease and with low-density lipoprotein cholesterol levels of 3.37 mmol/L or greater (>/=130 mg/dL). INTERVENTION Unopposed micronized 17beta-estradiol (1 mg/d) or placebo. All women received dietary counseling. Women received lipid-lowering medication if their low-density lipoprotein cholesterol level exceeded 4.15 mmol/L (160 mg/dL). MEASUREMENTS The rate of change in intima-media thickness of the right distal common carotid artery far wall in computer image processed B-mode ultrasonograms obtained at baseline and every 6 months during the 2-year trial. RESULTS In a multivariable mixed-effects model, among women who had at least one follow-up measurement of carotid intima-media thickness (n = 199), the average rate of progression of subclinical atherosclerosis was lower in those taking unopposed estradiol than in those taking placebo (-0.0017 mm/y vs. 0.0036 mm/y); the placebo-estradiol difference between average progression rates was 0.0053 mm/y (95% CI, 0.0001 to 0.0105 mm/y) (P = 0.046). Among women who did not receive lipid-lowering medication (n = 77), the placebo-estradiol difference between average rates of progression was 0.0147 mm/y (CI, 0.0055 to 0.0240) (P = 0.002). Average rates of progression did not differ between estradiol and placebo recipients who took lipid-lowering medication (n = 122) (P > 0.2). CONCLUSIONS Overall, the average rate of progression of subclinical atherosclerosis was slower in healthy postmenopausal women taking unopposed ERT with 17beta-estradiol than in women taking placebo. Reduction in the progression of subclinical atherosclerosis was seen in women who did not take lipid-lowering medication but not in those who took these medications.
SiO2/Si interfacial structure on vicinal Si(100) studied with second-harmonic generation.

Surface second-harmonic generation (SHG) has been used to reveal the symmetry characteristics of buried interfaces on oxidized vicinal Si(100). For native oxides, onefold and threefold symmetries are prominent, arising from the presence of steps and ordered Si-O bonds in the vicinity of the steps, respectively. For thermal oxides grown at 900 o C, twofold symmetry is most prominent, consistent with the presence of highly oriented microcrystallites at the buried interface. SHG measurements of postoxidation annealing of oxides grown in steam are consistent with the amount of stress reduction occurring as a result of annealing being too small to be observed by SHG, and with hydrogen leaving the buried interface below 600 o C
Exploring Children’s Creative Self-Efficacy Affected by After-School Program and Parent–Child Relationships

This study aimed to verify the relationship among children’s creative self-efficacy, parenting style, parent–child relationship, and after-school program. Judgmental sampling was used for subject selection from Taiwan. There are 550 valid participants composed of elementary school to junior high school students; their data were put into the statistical process. The multiple regression analysis was applied in this study. The survey tool was developed based on literature review and related articles. Research result supported the idea that the after-school program was the most significant variable that affected the student’s creative self-efficacy. The “punitive discipline” and “autonomy support” of parenting style can affect positive parent–child relationships as well as students’ creative self-efficacy. Evidence supported the notion that “negative parent–child relationships” will not motivate students’ creative self-efficacy. Besides, the after-school program plays an important role in the students’ creative self-efficacy independently.
Community service, educational performance and social responsibility in Northwest China

The main goal of this paper is to analyse the effect of high school scholarships tied to community service on the development of secondary school students in Northwest China. Using data from three rounds of surveys of thousands of students in 298 classes in 75 high schools in Shaanxi province, the paper documents the implementation of the Compassionate Heart Scholars Program and evaluates the effect of the programme on the educational performance, self‐esteem, self‐efficacy and social responsibility of the participants. We present evidence that part of the protocol of the programme (which includes the nomination and election of programme participants) improves the academic performance and self‐esteem of those involved in the programme. The community service part of the programme is shown to raise the self‐efficacy and the sense of social responsibility of the programme participants. One striking result is that the test scores of the community service participants do not appear to be adversely affected, even though they spend considerable time doing community service. The findings of this study, therefore, suggest that adding extra‐curricular community service to school curricula may be a win–win–win strategy, for the students, for schools and for the local communities served.
Sensitive Determination of Metal Ions in Drinking Water by Capillary Electrophoresis Coupled with Contactless Conductivity Detection Using 18-Crown-6 Ether and Hexadecyltrimethylammonium Bromide as Complexing Reagents

A simple, economical, and sensitive capillary electrophoresis (CE) method integrated with capacitively coupled contactless conductivity detection was developed for the determination of metal ions such as K+, Na+, Mg2+, Sr2+, Ca2+ in drinking water. 18-Crown-6 ether and Hexadecyltrimethylammonium Bromide (CTAB) were employed as complexing reagents. The effects of electrolyte additives, citric acid buffer solution, and other separation conditions of CE were comprehensively investigated and carefully optimized. The best results were obtained in a running buffer solution composed of citric acid (12 mM), 18-crown-6 ether (0.2 mM), and CTAB (0.015 mM) at pH 3.5. Under these conditions, a complete separation of five metal ions was successfully achieved in less than 12 min. The limits of detection for the optimal procedure were determined to be in the range of 0.02 - 0.2 mg·L-1. The repeatability with respect to migration times and peak areas, expressed as relative standard deviations, was better than 2.3% and 5.1%, respectively. Evaluation of the efficiency of the methodology indicated that it was reliable for the determination of metal ions in six different brands of drinking water samples.
Interferon treatment in acute progressive and fulminant hepatitis.

A small proportion of patients with acute viral hepatitis run a progressive fulminant course ending in acute liver failure with encephalopathy, and with a mortality rate of 75-80%. In small children and pregnant women mortality is even higher. We have treated 32 patients of all ages with acute progressive and fulminant hepatitis over the last 7 years in an uncontrolled trial with human interferon-alpha (HulFN-alpha), with i.m. doses of 3 x 10(6) u/day (70,000 u/kg per day for infants) for 8 +/- 3 days (mean +/- SD.) In 17 patients hepatitis was due to hepatitis A virus, in 7 to hepatitis B virus, in 6 to non A-non B virus and in 1 case each to herpes and cytomegalovirus. Sixteen patients (50%) recovered including 9 of 22 (41%) who were in Grades III-IV coma when treatment was started. Only 1 of 8 children less than 4 years of age recovered, whereas 15 of 24 (62%) older children and adults survived. Two of three pregnant women with acute fulminant hepatitis survived. In patients who recovered, improvement was often noted on about the fifth day of IFN treatment: 9 of 16 patients died before completing 5 days of therapy. Our studies of the IFN system response to hepatitis viruses showed that the greater majority of patients produce IFN in the acute stage of the infection. However, a minority have a defective IFN response that is more severe and more common in progressive fulminant hepatitis, and in several of these patients IFN response was completely lacking. It is in these cases that IFN treatment is likely to have the greatest value. On the basis of these encouraging preliminary results, it is suggested that a well-controlled, double-blind study be done to evaluate the effectiveness of HulFN-alpha treatment when given early during the course of acute progressive viral hepatitis.
HSP-CBF Is an NF-Y-dependent Coactivator of the Heat Shock Promoters CCAAT Boxes*

The cellular response to toxic stimuli is elicited through the expression of heat shock proteins, a transcriptional process that relies upon conserved DNA elements in the promoters: the Heat Shock Elements, activated by the heat shock factors, and the CCAAT boxes. The identity of the CCAAT activator(s) is unclear because two distinct entities, NF-Y and HSP-CBF, have been implicated in the HSP70 system. The former is a conserved ubiquitous trimer containing histone-like subunits, the latter a 110-kDa protein with an acidic N-terminal. We analyzed two CCAAT-containing promoters, HSP70 and HSP40, with recombinant NF-Y and HSP-CBF using electrophoretic mobility shift assay, protein-protein interactions, transfections and chromatin immunoprecipitation assays (ChIP) assays. Both recognize a common DNA-binding protein in nuclear extracts, identified in vitro and in vivo as NF-Y. Both CCAAT boxes show high affinity for recombinant NF-Y but not for HSP-CBF. However, HSP-CBF does activate HSP70 and HSP40 transcription under basal and heat shocked conditions; for doing so, it requires an intact NF-Y trimer as judged by cotransfections with a diagnostic NF-YA dominant negative vector. HSP-CBF interacts in solution and on DNA with the NF-Y trimer through an evolutionary conserved region. In yeast two-hybrid assays HSP-CBF interacts with NF-YB. These data implicate HSP-CBF as a non-DNA binding coactivator of heat shock genes that act on a DNA-bound NF-Y.