Miracle-dz/UniFAR-FTUs huggingface dataset:
Correlation of -475 IL-2 Promoter Gene Polymorphisms and the Levels of Serum IL-2 on the Risk of Multiple Sclerosis.

Background The aim of present study is to asset the IL-2 promoter gene (SNP -475) as a candidate gene for multiple sclerosis (MS) susceptibility. Methods This study included 70 patients with relapsing - remitting multiple sclerosis (RRMS) and 50 healthy controls. Following the extraction of genomic DNA from peripheral blood, frequency of genotypes and alleles of SNP -475 was calculated using Restriction fragment length polymorphism-polymer chain reaction (RFLP-PCR) and then the results were analyzed statistically. Results The results revealed the unusual ratio for the heterozygous (AT) was 1.6972 indicating that heterozygous patients were at higher risk of multiple sclerosis than wild homozygous (AA), and homomutant (TT). The results show protective role for - 475 IL-2 promoter among individuals with multiple sclerosis, (O.R: 0.4872; C.I. 95%: 0.1617- 1.4680) and (O.R: 0.9275; C.I. 95%: 0.2476 - 3.4745) for both AA and TT genotypes, respectively. Conclusion Our results showed that in this population of Iraqi patients, the AT genotype / A allele of -475 IL-2 promoter gene SNP may include attributed factors for MS predisposition.
Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement

Cucumbers are rich in vitamins and minerals. The cucumber has recently become one of China’s main vegetable crops. More specifically, the adjustment of the Chinese agricultural industry’s structure and rapid economic development have resulted in increases in the planting area allocated to Chinese cucumber varieties and in the number of Chinese cucumber varieties. After complete sequencing of the “Chinese long” genome, the transcriptome, proteome, and metabolome were obtained. Cucumber has a small genome and short growing cycle, and these traits are conducive to the application of molecular breeding techniques for improving fruit quality. Here, we review the developments and applications of molecular markers and genetic maps for cucumber breeding and introduce the functions of gene families from the perspective of genomics, including fruit development and quality, hormone response, resistance to abiotic stress, epitomizing the development of other omics, and relationships among functions.
In Silico Study of Natural Xanthones as Potential Inhibitors of Alpha-Glucosidase and Alpha-Amylase

: Type 2 diabetes mellitus is a disease caused by insulin resistance. Many types of oral medications exist, but the effectiveness and side effects differ from patient to patient, so alternative drugs are still required. One compound group receiving much scientific interest regarding its antidiabetic potential is xanthones as potential alpha-glucosidase and alpha-amylase inhibitors. This study performed molecular docking simulations on all 515 natural xanthones with alpha-glucosidase and alpha-amylase as the protein targets. We found 31 unique ligands that comply, and the three best ligands per protein target were filtered based on how many active site residues the ligands interacted with the targets. The three best alpha-glucosidase inhibitors are 3,4,5,8-tetrahydroxy-1,2-diisoprenylxanthone, Polygalaxanthone V, and Polygalaxanthone VII. As for alpha-amylase, we found 1-O-primeverosyl-3,8-dihydroxy-5-methoxyxanthone, Garcimangosone C, and Mangostinone as the best inhibitors. The six chosen and standard ligands underwent 2 ns molecular dynamics simulations. Both standard ligands had the highest interaction energies, followed by complexes with glycosylated xanthones, and prenylated xanthones. We also found that the prenylated xanthones could retain their initial protein-ligand interactions. Therefore, this is the first study that revealed prenylated xanthones have a good potential as anti-type 2 diabetes mellitus agents among other xanthones groups through in silico method.
Anxiety towards COVID-19, Fear of Negative Appearance, Healthy Lifestyle, and Their Relationship with Well-Being during the Pandemic: A Cross-Cultural Study between Indonesia and Poland

The COVID-19 pandemic has led to massive changes in almost all aspects of human life, including emotional states such as anxiety and fear, perspectives about healthy lifestyles, and psychological outcomes. This study aimed to disentangle the mechanisms that underlie the relationships of anxiety towards COVID-19 and fear of negative appearance with well-being, we also investigated the effects of cultural variations on levels of anxiety, fear of negative appearance, healthy lifestyles, and well-being. A total of 881 Indonesians (n = 172) and Poles (n = 709) participated in this study. Participants completed self-report measures of psychological well-being, anxiety, fear of negative appearance, compulsive exercise, and eating disorders. Multigroup structural equation modelling (SEM) was used. The results showed no statistically meaningful relationship between anxiety towards COVID-19 and well-being. However, it was found that, in the Polish sample, compulsive exercise and eating disorders mediated the relationship between fear of negative appearance and well-being. Cultural differences were also found in the mean scores of all examined constructs, with eating disorders being an exception. Therefore, this study highlights cultural aspects that determine emotional states, healthy lifestyles, and well-being.
Developing and Implementing an AI-Based Leak Detection System in a Long-Distance Gas Pipeline

This research proposes an artificial intelligence (AI) detection model using convolutional neural networks (CNN) to automatically detect gas leaks in a long-distance pipeline. The change of gap pressure is collected when leakage occurs in the pipeline, and thereby the feature of gas leakage is extracted for building the CNN model. The gas leak patterns in the long-distance pipeline are analyzed. A pipeline detection model based on AI technology for automatically monitoring the leaks is proposed by extracting the feature of gas leakage. This model is tested by collecting gas pressure data from an existing natural gas pipeline system starting from Mailiao to Taoyuan in Taiwan. The testing result shows that the reduced model of leak detection can be used to detect the leaks from the upstream and downstream pipelines, and the AI-based pipeline leak detection system can obtain a satisfactory result.
Melatonin Induced Schwann Cell Proliferation and Dedifferentiation Through NF-ĸB, FAK-Dependent but Src-Independent Pathways.

Background Peripheral nerve injury (PNI) is a common condition that compromises motor and sensory functions. Peripheral nerves are known to have regenerative capability and the pineal hormone, melatonin, is known to aid nerve regeneration. However, the role of Schwann cells and the pathways involved remain unclear. Thus, the aim of this study is to identify the effects of melatonin on Schwann cell proliferation, dedifferentiation, and the involvement of nuclear factor kappa light chain enhancer of activated B cells (NF-ĸB), focal adhesion kinase (FAK) and proto-oncogene tyrosine-protein kinase, Src pathways in this process. Methods Schwann cells was treated with melatonin and its proliferation and dedifferentiation were identified using MTT assay and immunofluorescence staining for SRY (sex determining region Y)-box 2 (SOX2). Next, the protein expressions of NF-ĸB, FAK and Src pathways were identified by Western blot. Results MTT results confirmed increased proliferation of Schwann cells with melatonin treatment, and it was highest at 10 μM melatonin. Immunofluorescent staining revealed an increase in the green fluorescence staining for SOX2 in melatonin-treated cells, showing enhanced dedifferentiation. Western blot assay revealed melatonin increased phospho-NF-ĸB (PNF-ĸB), IKK-α, FAK (D2R2E), phospho-FAK (Tyr 576/577 and Tyr 397) protein expressions as compared with control. However, Src (32G6), Lyn (C13F9), Fyn, Csk (C74C1) protein expressions were not increased as compared with control. Conclusion Melatonin promotes Schwann cell proliferation and dedifferentiation via NF-ĸB, FAK-dependent but Src-independent pathways.
Examining guests’ experience in luxury hotels: evidence from an emerging market

ABSTRACT Consumer experience has long been recognised as a key component in influencing consumers’ habits and attracting, pleasing, and retaining customers. The hospitality sector is often regarded as one of the most experience-rich industries. Lately, the idea of a guest’s experience in a luxury hotel has gained popularity. Limited studies have examined the antecedents and outcomes of luxury hotels guests’ experiences. This research bridges the void by constructing relationships among cognitive, affective, and behavioural constructs. A total of 414 guests in luxury hotels were contacted, to figure out factors influencing the guest experience. Structural equation modelling (SEM) was used to analyse the data. According to the results, hedonism, ambiance, escapism, personalisation, and convenience influence guests’ experience in luxury hotels, which in turn influence consumers’ positive word of mouth (WOM) and revisit intention. In addition, the moderating effect of the guests’ visit’s purpose was also examined.
Halogen-Based 17β-HSD1 Inhibitors: Insights from DFT, Docking, and Molecular Dynamics Simulation Studies

The high expression of 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) mRNA has been found in breast cancer tissues and endometriosis. The current research focuses on preparing a range of organic molecules as 17β-HSD1 inhibitors. Among them, the derivatives of hydroxyphenyl naphthol steroidomimetics are reported as one of the potential groups of inhibitors for treating estrogen-dependent disorders. Looking at the recent trends in drug design, many halogen-based drugs have been approved by the FDA in the last few years. Here, we propose sixteen potential hydroxyphenyl naphthol steroidomimetics-based inhibitors through halogen substitution. Our Frontier Molecular Orbitals (FMO) analysis reveals that the halogen atom significantly lowers the Lowest Unoccupied Molecular Orbital (LUMO) level, and iodine shows an excellent capability to reduce the LUMO in particular. Tri-halogen substitution shows more chemical reactivity via a reduced HOMO–LUMO gap. Furthermore, the computed DFT descriptors highlight the structure–property relationship towards their binding ability to the 17β-HSD1 protein. We analyze the nature of different noncovalent interactions between these molecules and the 17β-HSD1 using molecular docking analysis. The halogen-derived molecules showed binding energy ranging from −10.26 to −11.94 kcal/mol. Furthermore, the molecular dynamics (MD) simulations show that the newly proposed compounds provide good stability with 17β-HSD1. The information obtained from this investigation will advance our knowledge of the 17β-HSD1 inhibitors and offer clues to developing new 17β-HSD1 inhibitors for future applications.
Spin Polarization of Electrons in Two-Color XUV + Optical Photoionization of Atoms

The spin polarization of photoelectrons in two-color XUV + optical multiphoton ionization is theoretically considered using strong field approximation. We assume that both the XUV and the optical radiation are circularly polarized. It is shown that the spin polarization is basically determined by the XUV photoabsorption and that the sidebands are spin polarized as well. Their polarization may be larger or smaller than that of the central photoelectron line depending on the helicity of the dressing field.
Design and Control of a Quasi-Direct Drive Robotic Gripper for Collision Tolerant Picking at High Speed

Faster robotic picking of objects can improve industrial production throughput. A gripper design with appropriate control strategy is demonstrated to pick objects at high speed, while being tolerant to unintentional collisions. The design has a quasi–direct drive with backdrivable, rigid finger mechanism to transfer collision forces with minimal delay towards the motor side. These forces are detected based on band–pass filtered momentum and motor speed monitoring. The motor velocity is observed through Kalman filtering in order to reduce noise in the low–level control loop and band–pass momentum observer, resulting in a higher collision detection sensitivity. On a higher level, the path of the gripper is planned with respect to the trade–off picking speed versus collision tolerance, quantified by the remaining gripper stroke upon impact. The gripper collision tolerance was experimentally verified in several scenarios during high–speed object picking where it mitigated collisions up to 0.6 m/s.
Genome-Wide Identification of Binding Sites for SmTCP7a Transcription Factors of Eggplant during Bacterial Wilt Resistance by ChIP-Seq

Teosinte branched 1/cycloidea/proliferating cell factor (TCP) transcription factors play a key role in the regulation of plant biotic and abiotic stresses. In this study, our results show that SmTCP7a positively regulated bacterial wilt that was caused by Ralstonia solanacearum. ChIP-seq was conducted to analyze the transcriptional regulation mechanism of SmTCP7a before (R0 h) and 48 h after infection (R48 h). SmTCP7a regulated a total of 92 and 91 peak-associated genes in R0 h and R48 h, respectively. A KEGG (Kyoto encyclopedia of genes and genomes) pathway analysis showed that phenylpropanoid biosynthesis, MAPK (mitogen-activated protein kinas) signaling pathway, plant hormone signal transduction and plant-pathogen interactions were involved. The difference in peaks between R0 h and R48 h showed that there were three peak-associated genes that were modulated by infection. A better understanding of the potential target genes of SmTCP7a in response to R. solanacearum will provide a comprehensive understanding of the SmTCP7a regulatory mechanism during the eggplant defense response to bacterial wilt.
IgG from Adult Atopic Dermatitis (AD) Patients Induces Nonatopic Neonatal Thymic Gamma–Delta T Cells (γδT) to Acquire IL-22/IL-17 Secretion Profile with Skin-Homing Properties and Epigenetic Implications Mediated by miRNA

γδT cells mature in the human thymus, and mainly produce IL-17A or IFN-γ, but can also produce IL-22 and modulate a variety of immune responses. Here, we aimed to evaluate whether IgG from AD patients (AD IgG) can functionally modulate thymic nonatopic γδT cells. Thymic tissues were obtained from 12 infants who had not had an atopic history. Thymocytes were cultured in mock condition, or in the presence of either AD IgG or therapeutic intravenous IgG (IVIg). Following these treatments, intracellular cytokine production, phenotype, and microRNA expression profiles were investigated. AD IgG could downregulate α4β7, upregulate CLA, and induce the production of IFN-γ, IL-17, and IL-22 in γδT cells. Although both AD IgG and IVIg could directly interact with γδT cell membranes, AD IgG could reduce γδT cell apoptosis. AD IgG could upregulate nine miRNAs compared to IVIg, and six when compared to the mock condition. In parallel, some miRNAs were downregulated. Target gene prediction and functional analysis indicated that some target genes were enriched in the negative regulation of cellular transcription. This study shows that AD IgG influences the production of IL-17 and IL-22 by intrathymic nonatopic γδT cells, and demonstrates epigenetic implications mediated by miRNAs.
DAN EMPIRICAL ANALYSIS OF GRAPH ALGORITHM IN CONTEXT OF FREQUENT SUBGRAPH MINING ON GIRAPH SYSTEM

The Recurrent Subgraph Extraction plays a key role in the Graph Mining field when our data is distributed over networks. This paper emphasizes different types of graph mining algorithms with the Giraph Distributed System to get more desirable and valuable results than existing methods. We discuss how our proposed model MapReduce Geometric Multi-way Advanced Optimized Frequent Subgraph Mining (MGMAOFSM) impacts different graph mining mechanisms for centralized and distributed systems. The comparison is done for different criteria such as memory requirement or execution time with real four datasets (Facebook Social Network, Coronavirus (COVID-19) tweets, Google web graph, Patent Citation Network) with different threshold values. We implement various algorithms such as Triangle Closing, Shortest Path, Connected Components, and PageRank algorithms, and find out our proposed algorithm that requires less memory with the Triangle closing algorithm whereas in the case of PageRank is lowest with all threshold values.
Genetic Diversity Analysis of the Chinese Daur Ethnic Group in Heilongjiang Province by Complete Mitochondrial Genome Sequencing

Mitochondrial DNA (mtDNA) has the characteristics of maternal inheritance, high mutation rate, high copy number, and no recombination. As the most powerful tool for studying the origin and evolution of modern humans, mtDNA has great significance in the research of population genetics and evolutionary genetics. Here, we provide new insights into the maternal genetic history of the Daur ethnic group by generating complete mitochondrial genomes from a total of 146 Daur individuals in China. We also collected the published complete mitochondrial genome sequences of 5,094 individuals from 56 worldwide populations as reference data to further explore the matrilineal genetic landscape of the Daur ethnic group. First, the haplotype diversity was 0.9943 ± 0.0019 and nucleotide diversity was 0.0428 ± 0.0210. The neutrality tests of the Daur group showed significant negative values and the mismatch distribution curve was obviously distributed in a unimodal pattern. The results showed that the Daur ethnic group has high genetic diversity and may have experienced recent population expansion. In addition, the main haplogroups of the Daur population were haplogroup D (31.51%), M* (20.55%), C (10.28%), F (7.53%), and B (6.85%), all of which were prevalent in northern China. It probably implies the northern Chinese origin of the Daur population. The PCA, F ST, and phylogenetic analysis results indicated that the Daur group formed a cluster with East Asian populations, and had few genetic differences with the populations in northern China. More importantly, we found that disease-related mutation sites of the mitochondrial genome may be related to ethnic groups, which may have important implications for the prevention and occurrence of specific diseases. Overall, this study revealed the complexity and diversity of the matrilineal genetic background of the Daur ethnic group. Meanwhile, it provided meaningful data for the research on the diversity of the human genome.
Protein Markers Related to Non-halal Slaughtering Process of Rat as Mammal Animal’s Model Detected Using Mass Spectrometry Proteome Analysis

Meat produced from non-halal slaughter is forbidden for Moslems. The slaughter methods contribute to the physiological response of animals expressed as different proteome profile. Proteome of two meat obtained from the halal and non-halal slaughter of Wistar rats (Rattus norvegicus) as an animal model was used to search for protein markers related to the slaughter method. The analysis employed Sodium Dodecyl Sulhate Polyacrylamide Gel Electrophoresis (SDS-PAGE), and High-Resolution Mass Spectrometer (HRMS) assisted with Label-Free Quantification (LFQ) Proteome Discoverer software. The non-halal slaughter contributed to the changes in protein expression in animal meat where thirteen proteins were up-regulated and three proteins were specifically identified in the non-halal slaughter, these three proteins are NSFL1 cofactor p47, transketolase, and Von Willebrand. There are three stable peptides identified of those three proteins, SYQDPSNAQFLESIR (m/z = 1755, z = +1) part of NSFL1 cofactor p47, LGQSDPAPLQHQVDVYQK (m/z = 2023, z = +1) part of transketolase, VPLLCTNGSVVHHEVINAMQCR (m/z = 2550, z = +1) part of Von Willebrand. Two of peptides can be targeted as markers in MRM mode LC-MS/MS routine analysis to authentication-halal slaughtering meat. The proposed MRM pair ions are 1755 to 1376, 1262, 1164, and 963, 2033 to 1355, 1016, and 762.
Capacitive Power Transfer with Advanced Compensation and Power Flow Control

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Unravelling Facebook: a pedagogical tool during the Syrian crisis

ABSTRACT This paper aims to analyse the role of Facebook in academia during the Syrian crisis. Data were collected by means of an online survey from 726 students at Damascus University (Males = 446, Females = 280). Results indicated that Facebook facilitated communication and resource sharing in times of war and crisis where safe commuting posed a challenge for students. It served as an alternate trustworthy medium within the failure of the official web sites at Damascus University. The study suggests that Facebook is an effective pedagogical tool for countries going through crisis and war situations, and a promising educational tool that warrants further attention in underdeveloped and Arab countries in general.
Assessment of vitamin D level in women with abnormal uterine bleeding and chronic psychogenic stress

The objective: to study the level of vitamin D (25-hydroxycalciferol – (25(OH)D) in blood serum of women with abnormal uterine bleeding (AUB) and chronic psychogenic stress.Materials and methods. We examined 100 women of reproductive age with AUB and chronic psychogenic stress (basic group) and 50 patients with AUB without psychogenic chronic stress (comparison group). 30 women without AUB and chronic stress were included in the control group. AUB was diagnosis according to the Order No. 353 of the Ministry of Health of Ukraine dated April 13, 2016. The concentration of vitamin D was determined in blood serum in all the women using the ELISA method.Results. The concentration of vitamin D in the blood serum in patients with AUB and chronic psychogenic stress was on 28.01 % lower compared to the controls (p < 0.001), in the patients with AUB without psychogenic chronic stress – 13.94 % (p = 0.045). The number of individuals with optimal concentration of vitamin D in the control group (36.67 %) was in 2.29 times higher compared to the basic group (16.00 %; p=0.03) and in 1.41 times more compared to the comparison group (26.00 %). Suboptimal level of vitamin D had 53.33 % women in the control group, 43.00 % – basic and 58.00 % – comparison one. The rate of persons with deficiency of vitamin D among the patients with AUB and chronic psychogenic stress was in 4.56 times higher compared to the healthy women (41.00 % and 9.00 % individuals, respectively; p = 0.003), and in patients with AUB without chronic stress –1.78 time (16.00 % women). Conclusions. There is the insufficient vitamin D concentration in women with abnormal uterine bleeding which is more severe in the persons with chronic psychogenic stress.