Miracle-dz/UniFAR-FTUs huggingface dataset:
In Silico Prediction of the Phosphorylation of NS3 as an Essential Mechanism for Dengue Virus Replication and the Antiviral Activity of Quercetin

Simple Summary Dengue is a mosquito-borne virus that infects up to 400 million people worldwide annually. Dengue infection triggers high fever, severe body aches, rash, low platelet count, and could lead to Dengue hemorrhagic fever (DHF) in some cases. There is currently no cure, nor a broadly effective vaccine. The interaction of two viral proteins, nonstructural Proteins 3 and 5 (NS3 and NS5), is required for viral replication in the infected host’s cells. Our computational modeling of NS3 suggested that phosphorylation of a serine residue at position 137 of NS3 by a specific c-Jun N-terminal kinase (JNK) enhances viral replication by increasing the interaction of NS3 and NS5 through structural changes in amino acid residues 49–95. Experimental studies have shown that inhibition of JNK prevents viral replication and have suggested that the plants’ flavonoid Quercetin, Agathis flavone, and Myricetin inhibit Dengue infection. Our molecular simulations revealed that Quercetin binds NS3 and obstructs serine 137 phosphorylation, which may decrease viral replication. This work offers a molecular mechanism that can be used for anti-Dengue drug development. Abstract Dengue virus infection is a global health problem for which there have been challenges to obtaining a cure. Current vaccines and anti-viral drugs can only be narrowly applied in ongoing clinical trials. We employed computational methods based on structure-function relationships between human host kinases and viral nonstructural protein 3 (NS3) to understand viral replication inhibitors’ therapeutic effect. Phosphorylation at each of the two most evolutionarily conserved sites of NS3, serine 137 and threonine 189, compared to the unphosphorylated state were studied with molecular dynamics and docking simulations. The simulations suggested that phosphorylation at serine 137 caused a more remarkable structural change than phosphorylation at threonine 189, specifically located at amino acid residues 49–95. Docking studies supported the idea that phosphorylation at serine 137 increased the binding affinity between NS3 and nonstructural Protein 5 (NS5), whereas phosphorylation at threonine 189 decreased it. The interaction between NS3 and NS5 is essential for viral replication. Docking studies with the antiviral plant flavonoid Quercetin with NS3 indicated that Quercetin physically occluded the serine 137 phosphorylation site. Taken together, these findings suggested a specific site and mechanism by which Quercetin inhibits dengue and possible other flaviviruses.
“Yay, Another Lady Starting a Log!”: Women’s Fitness Doping and the Gendered Space of an Online Doping Forum

This study aims to investigate and dissect the meanings attached to women’s use of performance and image enhancing drugs (PIEDs), how fitness doping can be understood in terms of gender and spatiality, and what implications this has for women’s communicative engagement with one another within an online forum. The study is based on a netnographic and qualitative methodology. Theoretically, it considers a women’s online forum for PIEDs and analyzes it as a community of practice (CofP) and a spatiality in which gender, bodies, and side effects are discussed and negotiated. The results show that although the women’s forum provides a space for women to share their own unique experiences, there is a limit to the extent to which the discussions mirror the experiences and experimentations of women. Instead, discussions are often dominated by men’s voices/experiences. This has two main implications. Firstly, the prevalence of men’s voices can block the development of a women’s CofP. Symbolically, men engage in a sort of cultural manspreading by encroaching on the women’s forum space. Secondly, it has implications for women’s PIED use and use practices. Women seeking out advice or the experiences of other women must navigate through and around men’s contributions.
Organizational value creation and information technologies in hospitals: the case of electronic patient records

What types of organizational value do information technologies such as Electronic Health Records (EHRs) create in hospitals? Building on results from multi-site empirical research, this paper explores this issue in the case of the French EHR. Considering, on the basis of a theory of “management tools”, the organizational changes induced by the implementation of EHR, it suggests that the organizational transformation expected by public decision makers is only starting. At the same time, it argues that an enriched conceptualization of the notion of “organizational value” could make it possible to identify and manage some weak signals differently, thus indicating that on-going collective learning is likely to reconfigure practices and work processes. Departing from a dominant view focused on the improvement of care quality, security and efficiency, this article thus contributes to enriching the notion of the “organizational value” of information technologies in hospitals. Key-words organizational value, learning, Information Technology, organizational transformation, hospitals
Thesis #12 Report for CDSF (e-Horizon project)

With the tremendous growth of services and applications highly uploadingdata as much as downloading it, such as video conferencing and Virtual Real-ity (VR) streaming, the uplink throughput monitoring is required. However,throughput is considered as a crucial QoS metric in cellular networks. Infact, the chaotic radio phenomena characterizing the real environment leadto a degradation for the different QoS metrics, especially the throughput.Such phenomena include noise, multipath fading, and interference, etc. It isincreasingly hard to understand how the throughput changes rapidly depend-ing on the environmental situations. Therefore, radio phenomena should beconsidered when monitoring the uplink throughput in such environments. Asrecent researchers endorse the use of 4G standard for the short and middleterm services/applications, the uplink throughput monitoring in such net-works is then considered.
Efficacy and Safety of Tanshinone for Chronic Kidney Disease: A Meta-Analysis

Objective To systematically evaluate the efficacy and safety of tanshinone for chronic kidney disease (CKD). Methods Randomized controlled trials (RCTs) on the treatment of CKD using tanshinone were searched using 4 Chinese databases (China National Knowledge Infrastructure (CNKI), Value In Paper (VIP), Wanfang, and Chinese Biology Medicine (CBM)) and 3 English databases (PubMed, Cochrane Library, and Excerpta Medica Database (Embase)). The results included data on blood urine nitrogen (BUN), serum creatinine (Scr), glomerular filtration rate (GFR), 24 h urine protein, microalbuminuria (mALB), β2-macroglobulin (β2-MG), cystatin C (CysC), and safety events. The data were analyzed using Revman 5.3 and Stata 12.0 software. Results Twenty-one studies were entered into this meta-analysis, which involved 1857 patients including 954 cases from the tanshinone treatment group and 903 cases from the control group. BUN levels in the tanshinone treatment group were significantly reduced compared with the control (standardized mean difference (SMD) = −0.65, 95% confidence interval (CI): −0.81 to −0.49, p < 0.01). In addition, subgroup analysis indicated that tanshinone had a significant effect in reducing Scr levels at 14, 21, and 28 days. Scr levels in the tanshinone treatment group were significantly reduced compared with the control group (SMD = −1.40, 95% CI: −2.09 to −0.71, p < 0.01); subgroup analysis based on treatment time also yielded the same results. GFR in the tanshinone treatment group was better than that in the control group (SMD = 0.83, 95% CI: 0.59 to 1.07, p < 0.01). In terms of urine protein levels, 24 h urine protein level, mALB, and β2-MG of CKD patients were reduced to some degree compared with controls, and CysC levels in the tanshinone treatment group were also significantly reduced compared with the control group (SMD = −0.24, 95% CI: −0.44 to −0.03, p < 0.05). Safety in the tanshinone treatment group did not differ significantly from that of the control group (risk ratio (RR) = 7.78, 95% CI: 0.99 to 61.05, p > 0.05). Conclusion This meta-analysis showed that tanshinone could control urine protein level in CKD patients, improve kidney function, and delay the evolution of CKD without significant side effects. However, the results were limited and should be interpreted with caution because of the low quality of the included studies. In the future, more rigorous clinical trials need to be conducted to provide sufficient and accurate evidence.
Changes of Selected Egg Quality Traits Depending on the Laying Period of Semi-Intensively Raised Guinea Fowl Hens

The aim of this study was to evaluate selected egg quality traits depending on the laying period of pearl grey guinea fowl hens reared in semi-intensive system. In each of three evaluation terms (the second, fourth and sixth month of laying period) 40 eggs were collected, with the total of 120 eggs were used for quality evaluation. Methods of descriptive analysis and one-way ANOVA were used for data analysis. Overall values of three evaluation terms for egg weight, shell thickness, yolk to albumen ratio, Haugh units and yolk colour were 40.63 ± 0.27 g, 0.50 ± 0.01 mm, 0.58 ± 0.01, 75.37 ± 0.41 and 12.74 ± 0.11, respectively, whereas overall values of shell, albumen and yolk proportion were 15.32 ± 0.15, 53.75 ± 0.16 and 30.94 ± 0.17%, respectively. Significant differences were found for values of egg quality traits depending on the laying period, but the average value was generally comparable with literature data reported for similar rearing conditions for this Guinea fowl variety.
Stock assessment of pelagic fish in the eastern part of Java Sea: A case study in offshore Regency of Rembang and Tuban, Indonesia

Total annual landing of pelagic fish resources from the eastern part of Java Sea tend to be increased, but the stock status is not known well. This study was aimed to analyse optimal exploitation and stock status of pelagic fish in the eastern part of Java Sea and enlighten overfishing issues. Monthly catch of pelagic fish and effort data (trips) based on fishing gear during the period 2010 to 2016 were collected from four representative fish landing base or fishing port (i.e. Tasik Agung, Sarang, and Karang Anyer of Rembang Regency, and Bulu of Tuban Regency). Analyses were carried out using a surplus production model, and the best fit estimation was the Schaefer Model with Fox algorithm approach. Estimated bio-technique parameter of pelagic fish stock such as intrinsic growth rate (r) was 0.27 ton per year, catchability coefficient (q) was 0.000017 ton per unit, and environmental carrying capacity (K) was 622,614.72 ton per year. Result also showed that optimal production (h), effort (E) and profit (π) at Maximum Sustainable Yield (MSY) level 42,553.58 ton per year, 8,138 trips per year, and 263,783 billion rupiah (IDR), respectively. Meanwhile, those three variables at Maximum Economic Yield (MEY) were 41.670,87 ton per year, 6,966 trips per year, and 271,468 billion rupiah. However, the average of actual production, effort, and profit were 40,409.83 ton per year, 6,972 trips per year, and 260,408 billion rupiah. Moreover, total production and effort in 2016 were 42,009 ton and 10,114 trips. The status of pelagic fish stock in this area tends to be economic overfishing due to increased effort and less effective of fishing cost. Reducing fishing effort must be applied in fisheries management.
Are high school teachers and university academics on the same page? A case of the university readiness of business education learners at two schools and two universities

Many studies nationally and internationally continue to find that schools are not preparing learners adequately to cope with the demands of higher education, hence the high dropout rate particularly at the first-year level. We found that, in most cases, research studies conducted in South Africa focus solely on a particular stakeholder’s perception to gain insights into students’ readiness for university as opposed to corroborating perceptions of both teachers and academics. Thus, the aim of our paper is to explore the perceptions of both business education high school educators and lecturers in terms of how they view the readiness of learners for university, particularly for the bachelor of commerce degree programme. The findings highlight the lack of communication and collaboration between schools and universities. Recommendations are proposed to assist the staff of high schools and universities to work collaboratively in order to provide high school learners with adequate foundational knowledge and skills that will ensure that they are ready for higher education studies. We argue that exploring the perceptions of high school teachers and academics collectively could be a step in the right direction towards paving the way for successful learning at university for first-year students in bachelor of commerce degree programmes.
Origini e sviluppo dell'anatomia patologica a Trieste: Una storia paradigmatica

The history of the Italian city of Trieste is somewhat unique. In 1382, being unable to compete with nearby Venice, it gave itself up to the Archduchy of Austria, which, while ensuring Trieste a certain degree of autonomy, did nothing to improve the town's economy. Things changed, however, after 1719, when Trieste was declared a Free Port. The city, at the time poor and lacking the human and economic resources that might have given life to the long-awaited trading activities, started to attract flocks of immigrant labourers and professionals, including many physicians. Thus, from mid-18th C onwards, Trieste witnessed the rise of a local class of physicians. Graduates from the major European universities of the times, these physicians were highly active in the rapid professional and cultural growth of the city. Being free from traditions or affiliations to specific medical schools, this unusually multicultural class of professionals provides a sample of the times' medical class which is particularly suitable for understanding what schools of thought dominated during that period. A number of publications, largely dealing with pathology, and many historical circumstances show that this newly formed class of physicians was influenced by the scientific method advanced by Morgagni in his De Sedibus. It is also clear that Morgagni's influence greatly benefited the city's doctors and soon turned them into fertile and original promoters of ideas, as well as highly skilled professionals. When the times became ripe to take on a specialist in pathology, both medical class and local authorities showed great interest in the field by making sure that the successful candidate, Dr. Simon Pertot, had all the resources and equipment necessary for the specialist to become a central figure in professional training and medical research in Trieste. Thus, the kind of clinical pathology where a single physician performed macroscopic examinations to identify his patient's cause of death gave way to a more specialised discipline which was able to transfer--to post-mortem examinations first and soon after to surgically removed tissues--the wealth of notions developed in the middle of the 19th C in the basic sciences of chemistry, microbiology and physiology, as had been advanced by the second founding father of the discipline, Virchow. Trieste, therefore, may be said to offer a unique example of the origins and development of pathologic anatomy in Central Europe. And the recollection of the city's experience promotes, in the modern pathologist, an awareness of belonging to a discipline which has always been the soundest leader in the advancement of medicine.
Consumer behaviour factors and contemporary trends on the luxury goods market

Regardless of the economic crisis all over the world, the market of luxury goods thrives continuously. The research aims to provide an extensive overview of the theoretical background of this occurrence and contemporary trends, analyse the consumer behaviour factors in buying luxury goods and the effect of online shopping as a form of digitalisation on the sales of luxury goods, with particular emphasis upon the sale of luxury handbags. The methods were consisted of an overview of relevant scientific and expert literature, reports of market experts for the luxury goods markets and official statistic databases. Research resulted in detecting digitalisation as the biggest change and trend of luxury industry. Online sales and revenues of luxury goodsâ€™ continuous growth indicates that digitalisation and its tools such as online shopping are an important consumer behaviour factor, affecting the sales of luxury goods, including luxury handbags, to a significant extent.
RNA Isolation from Purified Rice Necrosis Mosaic Virus Particles

Rice, particularly grows in tropical and subtropical areas, is one of the most important crops worldwide. Rice necrosis mosaic virus (RNMV) was first reported in Japan and causes necrotic lesions on leaf and yellowing. This virus has been classified as member of the Potyviridae family's Bymovirus genus. The fungus, Polymixa graminis, transmits RNMV. In this study, the methodology first explained RNMV virus particle purification by ultra-centrifugation along with sucrose and cesium chloride gradient is described. Further, confirmation of purified RNMV particles under the electron-microscope is discussed. In the last RNMV RNA isolation from the purified RNMV particles were explained. Method Article Ganpatrao et al.; ACRI, 19(3): 1-6, 2019; Article no.ACRI. 53474 2
The Development Of Tracer Study System For PEP Alumni Graduate School Unnes By Online Based On The Website

The background of the study is needs of PEP study programs about alumni data and users for the necessity of the development of study programs and accreditation. The study aims to develop an effective way of tracer study, which is easy to use, easy to access information, and able to provide detailed feedback. The method of collecting data with distributed instruments in the form of open and closed questionnaires through the website that has been made. Research on tracer study alumni was conducted with online media including WhatsApp messenger, email, website and google form applications. The results of the study showed that the most complete information is collected by Google form and website. But google form has limitations because just be accessed by the admin using the email registered on the google form. The easiest system to access information, and able to provide detailed feedback, is the system who was developed online based on the website.
Design of Adaptive Planar Microstrip Patch Array Operating at 28 GHz for 5G Smart Mobile System

Smart antenna system has been studied extensively for the fifth generation of wireless communication systems, because it has made a system better performance of higher capacity and coverage as well as of power-saving. The present paper introduces a design of planar microstrip patch antenna array for a smart mobile system operating at 28 GHz. The present smart antenna has an adaptive radiation pattern that adjusts its main beam automatically to the desired direction by following the signal environment. This is based on the processing of an algorithm called the Least Mean Square (LMS) resulting in a change in the magnitude and phase of the feeding current for each element in the antenna array. From the obtained results, the main beam can be steered 180 degrees in the phi (azimuth) plane at a constant theta (elevation) angle. The planar antenna array was designed and simulated using CST Microwave Studio and MATLAB software that is used to find the required exciting current for each element. It is found that the antenna bandwidth is greater than 1 GHz while its gain is about 21 dB.
The Use of Mobile Wallet Services Apps in Urban Living

Urban life refers to people living in towns and cities, the most important feature of which is high mobility. Living in urban areas has been found to reduce leisure time because of a highly scheduled life, which means that many residents are now relying on technology to achieve their schedules. In this digital era, mobile applications such as mobile wallet services are making it easier for non-cash transactions, such as food and beverages, equipment, health, education, restaurants, hotels, transportation, communication, and others. This study used a quantitative approach using online questionnaires on 80 students living in the Jakarta Metropolitan Area; Jakarta, Bogor, Depok, Tangerang, and Bekasi; to assess their use of mobile wallet applications, from which it was found that the top three uses were for food and beverages, to top up phone credit, and for movies or concert tickets, and the top three factors influencing their adoption of mobile wallets services were the wallet promotions, and the easier and faster transactions. These perceived benefits of using mobile wallet services are developing a cashless society. Keywords—cashless society, digital mobile technology, mobile wallet services, mobility, urban life
Assessment of Heavy Metal Contamination in Waters due to Mineral Salts Company from Mighan playa/lake, Arak, Iran

The Mighan playa/lake is characterized as a closed catchment. In the recent years, the rapid industrialization and urbanization has resulted in a pollution area in the city of Arak. In this work, we focus on six regions around the playa/lake to study the distribution of heavy metals in the waters and their contamination risk. A total of 32 water samples are analyzed to determine the contamination degree of heavy metals, i.e. Hg, As, Cd, Cr, Cu, Pb, and Zn. The heavy metal pollution index, heavy metal evaluation index, and degree of contamination are utilized to assess the pollution extent of these metals. The spatial distribution patterns reveal that the waters in different areas of playa/lake are in a good condition. The island, lake in playa, and the Wastewater Mineral Salts Company are most seriously polluted with Pb, being higher than the standard of drinking water quality limit. Water in the wastewater treatment plant is polluted with Hg and As. The correlation matrix, factor analysis, and cluster analysis are used to support the idea that Pb may be mainly derived from the atmospheric input, and As and Hg from the wastewater treatment plant, agricultural lands, and domestic waste. Many native and migratory birds live in the Mighan playa, which is exposed to heavy metals. Therefore, it is required to monitor heavy metals in the Arak playa and to manage the municipal, industrial, and agricultural activities around it and to reduce them.