Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of m6A methyltransferase-related genes predicts prognosis and immune infiltrates in head and neck squamous cell carcinoma

Background Head and neck squamous cell carcinoma (HNSCC) accounts for 90% of head and neck malignant tumors. As the early symptoms of HNSCC are not obvious, and it is prone to recurrence and metastasis, making the overall survival (OS) rate of patients very low. Existing studies have shown m6A methylation plays a crucial role in various cancers, but it is rarely studied in HNSCC. This study aimed to explore the expression of m6A methylation-related genes in HNSCC and its correlation with prognosis, and to explore its relationship with immune infiltration. Methods The gene expression data of HNSCC patient tumor samples (tumor =510) and adjacent normal tissue samples (normal =50) were extracted from The Cancer Genome Atlas (TCGA) database, and the expression characteristics of m6A regulatory factors were described. Kaplan-Meier survival analysis was used to analyze the relationship between m6A regulatory factors and OS and disease-specific survival (DSS). Least absolute shrinkage and selection operator (LASSO) regression was used to construct the m6A regulatory factor-HNSCC risk prediction model. In addition, the relationship between m6A methylation-related genes and tumor immune infiltration were discussed. Results The differential expression of 20 genes were identified by TCGA, and 18 genes (IGF2BP2, IGF2BP1, IGF2BP3, VIRMA, YTHDF1, YTHDF2, YTHDF3, ZC3H13, METTL14, ALKBH5, METTL3, RBMX, WTAP, YTHDC1, FTO, HNRNPC, HNRNPA2B1, and RBM15) were overexpressed in HNSCC. The survival rate of different gene expression levels was different. The high expression of YTHDC1 and YTHDC2 indicated better OS. Furthermore, for DSS, increased expression of YTHDC2 was also correlated with better clinical outcomes (P<0.05). At the same time, we drew a 3-gene risk score model in the TCGA-HNSCC cohort, and the survival curve showed compared with low-risk patients, high-risk patients had significantly worse OS (P<0.05). Gene enrichment analysis showed EPITHELIAL_MESENCHYMAL_TRANSITIO, MTORC1_SIGNALING, MYC_TARGETS_V1, MYC_TARGETS_V2, MYOGENESIS pathways, high TP53 mutations, and suppressive immunity were related to the high-risk group. The low-risk group was related to ALLOGRAFT_REJECTION, COMPLEMENT, IL6_JAK_STAT3_SIGNALING, INTERFERON_ALPHA_RESPONSE, INTERFERON_GAMMA_RESPONSE pathways, low TP53 mutations, and active immunity. Conclusions The m6A methyltransferase-related genes can predict the prognosis of HNSCC and are related to immune infiltration.
Multi-omics analysis reveals cross-organism interactions in coral holobiont

Corals create an ecosystem, called a holobiont, with intracellular algae (zooxanthellae) and resident bacteria. Zooxanthellae and some bacteria play major roles in the physiological properties of the coral host. However, because of the difficulties in experimental verification of cross-organism interactions, the mechanisms underpinning these interactions are largely unknown. To address this, we here generated and then analyzed multi-omics datasets for corals, zooxanthellae, and bacteria collected at Okinawa, Japan, from November 2014 to September 2016. Using cross-organism co-expression analysis, we successfully characterized the host–alga relationship in the coral holobiont. Specifically, we observed that the coral host dominates the zooxanthellae. The multi-omics analysis also suggested that infection with coral-associated bacteria Endozoicomonas likely involves coral-like ephrin ligands, triggering an immune response of the coral host. This study highlights the potential of the multi-omics approach to elucidate coral–microbe interactions.
Comparative in vitro dissolution and in vivo bioavailability of commercial amlodipine tablets

Purpose: To evaluate the in vivo and in vitro behavior of amlodipine immediate release products. Methods: Three Mexican amlodipine products and the innovator (Norvasc ® ) were evaluated. Three bioequivalence studies were performed in 24 healthy male and female volunteers each. Plasma concentrations were determined using a liquid chromatographic method coupled with tandem mass spectrometry (LC/MS/MS). Dissolution profiles were evaluated using USP type apparatus 2 at 75 rpm and 500 mL of HCl 0.1N, pH 4.5 and pH 6.8. Also, the dissolution behavior of different lots of the innovator product was evaluated using apparatus 1 or 2 and 900 mL of buffer pH 6.8. Results: All the generic products under study were bioequivalent to the innovator. In vitro data showed that although at pH 1.2 and 4.5, the products met the specifications for very rapidly dissolving products but at pH 6.8, neither the innovator nor the test products complied with the criteria for rapidly dissolving products. When the study was performed at pH 6.8 in 900 mL of medium, the innovator showed a rapid dissolution behavior. Conclusion: The results show that the use of WHO conditions (900 mL of media, apparatus 2 at 75 rpm) are more adequate to predict the in vivo behavior of the amlodipine products. Keywords: Biopharmaceutics Classification System (BCS), Dissolution, Bioequivalence, Solubility, Permeability
The Robust Causal Relationships Among Domestic Tourism Demand, Carbon Emissions, and Economic Growth in China

Green and low-carbon development has become a compelling trend of our time. To formulate policies for development and also reduction of carbon emissions, quantifying the trend of tourism in green sustainable development is an essential issue for China, which is undergoing an economic transformation. This study first measured China’s domestic tourism carbon emissions through a bottom-up approach and then used the robust Granger causality test on annual data from 1993 to 2019 to investigate the relationships among China’s domestic tourism revenue, carbon emissions, and economic growth. The empirical results show that: (1) Carbon emissions of the domestic tourism industry are growing steadily, and the carbon emissions of the transportation industry determine the trend of the total carbon emissions of the domestic tourism industry, (2) long-term equilibrium relationships exist among China’s domestic tourism, carbon emissions, and economic growth, and (3) bidirectional causal relationships among economic growth, carbon emissions, and domestic tourism revenue have been detected with the robust Granger causality test, and the time-varying causal relationships may change markedly in these times of significant events and policy changes. Therefore, policymakers should coordinate the relationships among domestic tourism revenue, carbon emissions, and economic growth, in an effort to promote the development of sustainable tourism in China.
Chemical Composition of Saudi Arabian Sukkari variety of Date Seed Oil and Extracts Obtained by Slow Pyrolysis

The present study was designed to convert Saudi Arabian Sukkari waste date seeds into pyrolysis liquid char oil in a slow pyrolysis reactor and determine the phytoconstituents by gas chromatography/mass spectrometry. The date seed in particle form was pyrolysed in an externally heated pan sand bath to obtain 5.2 % v/w liquid char oil. The liquid char oil (20 ml) was dissolved in 100 ml aqueous methanol (30:70) and was four times fractionated with 25 ml of n-hexane. Small portion (1 g) of both methanol and n-hexane fraction was subjected to gas chromatography/mass spectrometry analysis. Phytochemical screening of both fractions revealed the presence of steroids, alkaloids, reducing sugars, phenolics, flavonoids, terpenoids, fatty acids and amino acids. The prevailing compounds found in n-hexane fraction were cyclolanost-24-en-3-ol (11.25 %), stigmast-5-en-3 β–ol (2.02%), lupen-3-one (3.50%), isopropyl myristate (0.64%), palmitic acid, methyl ester (0.74 %), oleic acid (0.77 %), tridecane (5.66 %), and dodecane (4.42 %). Compounds found in methanol fraction were mainly stigmast-5-en-3-ol (5.77 %), propylene glycol monooleate (2.82 %), guanosine (35.92 %), DL-arabinitol (2.11 %), dodecanoic acid (8.14 %), stearic acid (4.22 %), palmitic acid (7.61 %), oleic acid (9.13 %), 1,3,5-triazine-2,4,6-triamine (1.87 %) and 2-butylbutanoic acid (1.01 %). The presence of these bioactive compounds confirmed the application of the Sukkari varieties of date seeds for various medicinal activities in future drug discovery system and could be analyzed for antiinflammatory, antioxidant, cardiovascular, anticancer and immunosuppressant activities. However, isolation of individual phytochemical constituents may proceed to find a novel drug.
The stability estimates of the inverse problem for the weighted Radon transform

ABSTRACT In this paper, we get some new properties of the weighted Radon transform by Fourier transform, convolution, Riesz potential, and so on. Meanwhile, the results of Natterer are generalized to non-uniform attenuation. Furthermore we study the Sobolev estimation of the n-dimensional non-uniform attenuation Radon transform and its dual operator by the Young's inequality. Then, we extend conclusions of Rigaud and Lakhal to the n-dimensional space. Finally, the results of Sharafutdinov are generalized to non-uniform attenuation.
Measurements of neutron total and capture cross sections of 241Am with ANNRI at J-PARC

ABSTRACT Neutron total and capture cross sections of 241Am have been measured with a new data acquisition system and a new neutron transmission measurement system installed in Accurate Neutron Nucleus Reaction measurement Instrument at Materials and Life Science Experimental Facility of Japan Proton Accelerator Research Complex. The neutron total cross sections of 241Am were determined by using a neutron time-of-flight (TOF) method in the neutron energy region from 4 meV to 2 eV. The thermal total cross section of 241Am was derived with an uncertainty of 2.9%. A pulse-height weighting technique was applied to determine neutron capture yields of 241Am. The neutron capture cross sections were determined by the TOF method in the neutron energy region from the thermal to 100 eV, and the thermal capture cross section was obtained with an uncertainty of 4.1%. The evaluation data of JENDL-4.0 and JEFF-3.2 were compared with the present results.
Global cognitive dysfunction and β-amyloid neuropathology in late-life and treatment-resistant major depression

Abstract Background Cognitive impairment is common in late-life depression, which may increase Alzheimer disease (AD) risk. Therefore, we aimed to investigate whether late-life major depressive disorder (MDD) has worse cognition and increases the characteristic AD neuropathology. Furthermore, we carried out a comparison between treatment-resistant depression (TRD) and non-TRD. We hypothesized that patients with late-life depression and TRD may have increased β-amyloid (Aβ) deposits in brain regions responsible for global cognition. Methods We recruited 81 subjects, including 54 MDD patients (27 TRD and 27 non-TRD) and 27 matched healthy controls (HCs). Neurocognitive tasks were examined, including Mini-Mental State Examination and Montreal Cognitive Assessment to detect global cognitive functions. PET with Pittsburgh compound-B and fluorodeoxyglucose were used to capture brain Aβ pathology and glucose use, respectively, in some patients. Results MDD patients performed worse in Montreal Cognitive Assessment (p = 0.003) and had more Aβ deposits than HCs across the brain (family-wise error-corrected p < 0.001), with the most significant finding in the left middle frontal gyrus. Significant negative correlations between global cognition and prefrontal Aβ deposits existed in MDD patients, whereas positive correlations were noted in HCs. TRD patients had significantly more deposits in the left-sided brain regions (corrected p < 0.001). The findings were not explained by APOE genotypes. No between-group fluorodeoxyglucose difference was detected. Conclusions Late-life depression, particularly TRD, had increased brain Aβ deposits and showed vulnerability to Aβ deposits. A detrimental role of Aβ deposits in global cognition in patients with late-onset or non-late-onset MDD supported the theory that late-life MDD could be a risk factor for AD.
Trisomy 21 increases microtubules and disrupts centriolar satellite localization

Trisomy 21, the cause of Down syndrome, causes a 0.5-fold protein increase of the chromosome 21-resident gene Pericentrin (PCNT) and reduces primary cilia formation and signaling. Here we investigate the mechanisms by which PCNT imbalances disrupt cilia. Using isogenic RPE-1 cells with increased chromosome 21 dosage, we find PCNT protein accumulates around the centrosome as a pericentrosomal cluster of enlarged cytoplasmic puncta that localize along and at MT ends. Cytoplasmic PCNT puncta impact the intracellular MT trafficking network required for primary cilia, as the PCNT puncta sequester cargo peripheral to centrosomes in what we call pericentrosomal crowding. The centriolar satellite proteins, PCM1, CEP131 and CEP290, important for ciliogenesis, accumulate at sites of enlarged PCNT puncta in trisomy 21 cells. Reducing PCNT when chromosome 21 ploidy is elevated is sufficient to decrease PCNT puncta, reestablish a normal density of MTs around the centrosome, restore ciliogenesis to wild type levels and decrease pericentrosomal crowding. A transient reduction in MTs also decreases pericentrosomal crowding and partially rescues ciliogenesis in trisomy 21 cells, indicating that increased PCNT leads to defects in the microtubule network deleterious to normal centriolar satellite distribution. We propose that chromosome 21 aneuploidy disrupts MT-dependent intracellular trafficking required for primary cilia. TOC McCurdy et al explore why elevated Pericentrin in trisomy 21 negatively impacts primary cilia formation and signaling. They find that elevated Pericentrin produces more pericentrosomal puncta that associates with and increases microtubules. Changes to Pericentrin and microtubules mislocalizes centriolar satellites in a pericentrosomal crowd.
DFT Study To Explore the Importance of Ring Size and Effect of Solvents on the Keto–Enol Tautomerization Process of α- and β-Cyclodiones

We have explored the effect of ring size on keto–enol tautomerization of α- and β-cyclodiones using the M062X-SMDaq/6-31+G(d,p)//M062X/6-31+G(d,p) level of theory. The calculated results show that the activation free energy barrier for the keto–enol tautomerization process of α-cyclopropanedione (1) is 54.9 kcal/mol, which is lower compared to that of the other cyclic diketo systems studied here. The four-membered α- and β-cyclobutanedione (2 and 6) do not favor keto–enol tautomerization unlike other studied cyclic systems because of the ring strain developed in the transition-state geometries and their corresponding products. Water-assisted keto–enol tautomerization with one molecule reveals that the free energy activation barriers reduce almost half compared to those for the uncatalyzed systems. The two-water-assisted process is favorable as the activation free energy barriers lowered by ∼10 kcal/mol compared to those of the one-water-assisted process. The ion-pair formation seems to govern the lowering of activation barriers of α- and β-cyclodiones with two water molecules during the keto–enol tautomerization process, which however also overcomes the favorable aromatization in the three-membered ring system. The free energy activation barriers calculated with the M062X-SMDaq/6-31+G(d,p) level predicted that the keto–enol tautomerization process for the α-cyclodiones follows the following trend: 2 > 3 > 4 > 5 > 1. Water-assisted tautomerization of α-cyclodiones also predicted 1-W and 1-2W as the most favored processes; however, 5-W and 5-2W were found to be disfavored in this case. The β-cyclodione systems also showed similar trends as obtained with α-diketone systems. The influence of bulk solvent on the keto–enol tautomerization process favors the formation of the enol form in a more polar solvent medium even under mixed solvent conditions in acetonitrile and hexane at M062X-SMDacetonitrile/6-31+G(d,p) and M062X-SMDhexane/6-31+G(d,p) levels of theory.
Universal Rice Primer (URP) and Start Codon Target (SCoT) Markers in Studying Population Structures and Genetic Variation in Ferula AssafoetidaL. Accessions

Ferula assafoetidais an herbaceous, annual and monocarpic genus of the Apiaceae family. So far, there has been common usage of Ferula oleo resinous gum in food and herbal medicines.The origin of F.assafoetidais can be traced back into the steppes of Iran and some reigns of Afghanistan with an extended distribution. Despite the economic value and therapeutic importance of F.assafoetida, only a few studies have reported on the genetic capacity of this herb.The present study was carried out on a set of 90 individual plants belonging to different populations of Ferula assafoetida L. via the start codon target marker (SCoT) and the universal rice primer (URP) markers. Twelve SCoT and twelve URP primers generated 192 and 149 polymorphic fragments, while having 16 and 12.41 fragments respectively on average per primer. The Polymorphism information content (PIC) for URP primers and ScoT ranged from 0.31 to 0.43 and 0.34 to 0.44 respectively, which indicated a good efficiency forboth markers. The diversity indices including heterozygosity (He), percentage of polymorphic bands (PPB), Shannon’s information index (I) and marker index (MI) were calculated based on the SCoT and URP data.The results revealed that SCoT primers were more efficient than URP primers in identifying genetic diversity within populations. Neighbor joining (Nj), as a base for clustering, classified 90 accessions into 5 and 6 groups using SCoT and URP data respectively. Moreover, the combined data (SCoT+URP) succeeded in classifying all accessions into 6 groups, although this did not correspond with the geographical distribution of accessions. Structure analysis divided 90 genotypesinto 5 subpopulations using SCoT and URP markers, whereas the combined data (SCoT+URP) divided the accessions into 6 subpopulations, which confirmed the classification achieved by the Nj method. Principal coordinate analysis (PCoA) corroborated these conclusions. According to the analysis of molecular variance) AMOVA(, a high percentage of genetic diversity was found within the species, suggesting a rich diversity of germplasm for breeding plans. The assessment of population structure demonstrated a high rate of gene flow and the mixture of populations. Taken together, our findings suggest SCoT markers are more efficient than URP markers in assessing genetic diversity among F.assafoetida genotypes. All in all, genetic diversity could help the selection of appropriate markers and special genotypes for breeding plans.
Evaluating the rheological effect of asphalt binder modification using soybean oil

Abstract Brazil is a highlight in the ranking of maize cooking oil production, which after consumption generates a significant amount of waste that the improper disposal of it, which could seriously affect the sewage pipes, groundwater, and soil. This research study the incorporation of 1%, 2%, and 3% of soybean (new and waste) in asphalt binder. The samples were analyzed by FTIR and frequency sweep tests. The results indicate that the optimum oil content is in the range of 1–2%, regardless of being new or residual. In the temperature sweep, it was found that the asphalt binder 55/75-E showed a smaller phase angle in all mixtures compared to the asphalt binder 50/70, thus having a better elastic recovery.
Thermal Conductivity and Photothermal Conversion Performance of Ethylene Glycol-Based Nanofluids Containing Multiwalled Carbon Nanotubes

In this study, the successful hydroxyl functionalization of carbon nanotubes (MWCNT-OH) was prepared by chemical treatments and characterized by Raman and FTIR spectra. MWCNT-OH was successfully dispersed into ethylene glycol (EG) with the Tween-80 surfactant by an ultrasonication method to prepare nanofluids. Thermal conductivity of nanofluid was investigated, and a maximum thermal conductivity enhancement about 24% at 50°C was obtained with nanofluid containing 0.64 vol.% MWCNT concentration. The photothermal conversion performance of nanofluid was also investigated by measuring the change of the temperature under lighting from a solar simulator. The measurement results showed that the highest photothermal conversion was obtained about 4.2% after a 30 min lighting with nanofluid containing 0.48 vol.% CNT concentration compared to ethylene glycol. These obtained results have propose a potential application of CNTs in nanofluids for solar absorption.
A Multidisciplinary Telerehabilitation Approach for Supporting Social Interaction in Autism Spectrum Disorder Families: An Italian Digital Platform in Response to COVID-19

Due to its complexity and high variability in symptomology, autism spectrum disorder (ASD) requires a coordinated and multidisciplinary intervention to better support the different programs over time and to promote social interactions in all contexts of life. Telemedicine can offer a valuable contribution in this regard, providing low-cost and portable applications. In this paper, we presented an Italian project, SUPER, which aimed to foster collaboration and information sharing between ASD families, health services, and schools. SUPER provided a digital platform with several tools that were useful both to enhance general and specific ASD knowledge and to promote personalized programs for children with ASD. We conducted a preliminary user test for the platform with 30 participants (18 therapists and 12 parents of children with ASD) using the system usability scale (SUS). The total mean SUS score (89.2) showed that SUPER is an excellent, usable system. Moreover, we extracted the usability and learnability mean components from the SUS scores, which were 96.1 and 61.7, respectively. Our preliminary results indicate that SUPER is a very user-friendly application and its innovative telemedicine approach could be ahelpful communication and collaboration tool among the different contexts of care for children with ASD.
Study on Droplet Dispersion Influenced by Ventilation and Source Configuration in Classroom Settings Using Low-cost Sensor Network

The COVID‐19 virus can transmit through airborne expiratory droplets and thus, the viral transmission can take place between the occupants in the isolated room. With the school re-opening under the current COVID‐19 pandemic, it is urgent to improve the classroom ventilation system to mitigate the risk of virus transmission. The present study developed a particle concentration monitoring network (PCMN) using low‐cost sensors and deployed it to explore the dispersion of the droplet particles under different ventilation settings and aerosol configurations. Our experiment shows the advance of using a low‐cost sensor network on spatiotemporal air monitoring and demonstrates indoor particle concentration level and distribution are strongly impacted by the ventilation setting and source location. Two recommendations on reducing the viral risk in the classroom were derived from the study. The first is the respiratory droplet source, e.g., the instructor, should be in the location such that the particle dispersion opposes the ventilation flow. The second is the air handling unit (AHU) and fan coil unit (FCU) should be both turned on during class hours despite whether there is a need for thermal comfort, as it allows higher and more uniform ventilation flow to resolve the issue of the dead air zone. © The Author(s).