Miracle-dz/UniFAR-FTUs huggingface dataset:
N-substituted phenylbenzamides of the niclosamide chemotype attenuate obesity related changes in high fat diet fed mice

Obesity and insulin resistance are primary risk factors for Non-Alcoholic Fatty Liver Disease (NAFLD). NAFLD is generally exhibited by non-progressive simple steatosis. However, a significant subset of patient’s progress to nonalcoholic steatohepatitis (NASH) that is defined by the presence of steatosis, inflammation and hepatocyte injury with fibrosis. Unfortunately, there are no approved therapies for NAFLD or NASH and therefore therapeutic approaches are urgently needed. Niclosamide is an U.S. Food and Drug Administration (FDA)-approved anthelmintic drug that mediates its effect by uncoupling oxidative phosphorylation. Niclosamide and its salt forms, Niclosamide Ethanolamine (NEN), and Niclosamide Piperazine (NPP) have shown efficacy in murine models of diet induced obesity characterized by attenuation of the prominent fatty liver disease phenotype and improved glucose metabolism. While the exact mechanism(s) underlying these changes remains unclear, the ability to uncouple oxidative phosphorylation leading to increased energy expenditure and lipid metabolism or attenuation of PKA mediated glucagon signaling in the liver have been proposed. Unfortunately, niclosamide has very poor water solubility, leading to low oral bioavailability. This, in addition to mitochondrial uncoupling activity and potential genotoxicity have reduced enthusiasm for its clinical use. More recently, salt forms of niclosamide, NEN and NPP, have demonstrated improved oral bioavailability while retaining activity. This suggests that development of safer more effective niclosamide derivatives for the treatment of NAFLD and Type 2 Diabetes may be possible. Herein we explored the ability of a series of N-substituted phenylbenzamide derivatives of the niclosamide salicylanilide chemotype to attenuate hepatic steatosis using a novel phenotypic in vitro model of fatty liver and the high fat diet-fed mouse model of diet induced obesity. These studies identified novel compounds with improved pre-clinical properties that attenuate hepatic steatosis in vitro and in vivo. These compounds with improved drug properties may be useful in alleviating symptoms and protection against disease progression in patients with metabolic syndrome and NAFLD.
Modeling the Transient Behavior of MOS-Transistors during ESD and Disturbance Pulses in a System with a Generic Black Box Approach

On-chip ESD protection in smart power technologies is often done with MOSFETs, either self-protecting or as dedicated ESD-protection. Turned on by intrinsic gate-drain capacitive coupling they conduct dynamically channel current as well as additional avalanche current depending on MOSFET type. A generic approach to model this transient behavior for system ESD and disturbance pulse simulation is presented.
SSVEP Transient Feature Extraction and Rapid Recognition Method Based on Bistable Stochastic Resonance*

Steady-state Visual Evoked Potential, SSVEP), as the most commonly used communication paradigm for non-implantable Brain-Computer Interface (BCI), boasts the advantages of unnecessity of training, noise immunity and periodicity. The traditional SSVEP extraction methods can effectively identify the target frequency contained in original EEG, however, the required data length usually lasts a few seconds. In this paper, bistable stochastic resonance (BSR) is applied to SSVEP extraction. BSR is very sensitive to amplitude mutation and frequency fluctuation of the input signal, making the output difference can be used for the detection of the target frequency. The processing results illustrate that the proposed method not only has a high recognition accuracy, but also effectively shortens the recognition time, thus improving the calculating speed. Therefore, SSVEP extraction based on BSR has a higher information transmission rate (ITR), which is more suitable for the real-time BCI system.
Molecular Mechanisms of Short-Term Plasticity: Role of Synapsin Phosphorylation in Augmentation and Potentiation of Spontaneous Glutamate Release

We used genetic and pharmacological approaches to identify the signaling pathways involved in augmentation and potentiation, two forms of activity dependent, short-term synaptic plasticity that enhance neurotransmitter release. Trains of presynaptic action potentials produced a robust increase in the frequency of miniature excitatory postsynaptic currents (mEPSCs). Following the end of the stimulus, mEPSC frequency followed a bi-exponential decay back to basal levels. The time constants of decay identified these two exponential components as the decay of augmentation and potentiation, respectively. Augmentation increased mEPSC frequency by 9.3-fold, while potentiation increased mEPSC frequency by 2.4-fold. In synapsin triple-knockout (TKO) neurons, augmentation was reduced by 83% and potentiation was reduced by 74%, suggesting that synapsins are key signaling elements in both forms of plasticity. To examine the synapsin isoforms involved, we expressed individual synapsin isoforms in TKO neurons. While synapsin IIIa rescued both augmentation and potentiation, none of the other synapsin isoforms produced statistically significant amounts of rescue. To determine the involvement of protein kinases in these two forms of short-term plasticity, we examined the effects of inhibitors of protein kinases A (PKA) and C (PKC). While inhibition of PKC had little effect, PKA inhibition reduced augmentation by 76% and potentiation by 60%. Further, elevation of intracellular cAMP concentration, by either forskolin or IBMX, greatly increased mEPSC frequency and occluded the amount of augmentation and potentiation evoked by electrical stimulation. Finally, mutating a PKA phosphorylation site to non-phosphorylatable alanine largely abolished the ability of synapsin IIIa to rescue both augmentation and potentiation. Together, these results indicate that PKA activation is required for both augmentation and potentiation of spontaneous neurotransmitter release and that PKA-mediated phosphorylation of synapsin IIIa underlies both forms of presynaptic short-term plasticity.
Reactive Execution of Learned Tasks With Real-Time Collision Avoidance in a Dynamic Environment

This paper addresses the problem of learning from demonstration (LfD) and subsequent robot safety control in an unstructured dynamic environment different from the demonstrations. Generally, LfD has been successfully exploited for task programming, but the existing methods have not solved the problem of allowing the entire arm to avoid obstacles while satisfying the task motion constraints (e.g., the robotic arm approaching the target object while avoiding obstacles moving within the environment). To achieve this, we present an incremental LfD approach that combines a task-parameterized probabilistic model and the robot security domain to control a robot’s behavior during task execution. Specifically, we propose a safety-oriented and task-oriented control strategy for redundant manipulators that makes full use of the motion redundancy of the manipulator and the space with no task restraints to satisfy the task constraints for human-robot coexistence. We then demonstrate the effectiveness of the proposed approach through a series of pick-and-pour experiments performed by a manipulator with 7 degree of freedom in a dynamic environment, where the robot must both avoid obstacles and satisfactorily complete the learned task with constraints.
Supporting parental caregivers of children living with life‐threatening or life‐limiting illnesses: A Delphi study

BACKGROUND The impact of a child s life-limiting or life-threatening illness is significant on parents who experience a great deal of emotional, physical, and spiritual upheaval. Hope has been identified as an important inner resource for parental caregivers. Specifically, parental hope has been described as having four subproceses including Accepting Reality, Establishing Control, Restructuring Hope, and Purposive Positive Thinking. PURPOSE The purpose of this Delphi study was to gather expert opinions from parents and formal care providers about the four subproceses essential to parental hope, to increase understanding of parental caregivers current support needs. As Phase one of a three-phase study, the findings provided direction in the development of a theory-based hope intervention. DESIGN AND METHODS A Delphi study consisting of three rounds of survey questions and controlled feedback to experts was employed. Experts suggested strategies for each subprocess and ranked them in order of highest to lowest according to feasibility and effectiveness. RESULTS Sixty-eight experts consisting of parental caregivers of children diagnosed with life-limiting or life-threatening illnesses and those who care for them (community members, nurses, social workers, and physicians) were recruited to participate. Through three rounds of survey questions, response rates ranged from 92-97%. A consensus revealed eight major themes that support parental hope: Organize Basic Needs; Connect with Others; Prioritize Self-care; Obtain Meaningful Information; Take Things Day by Day; Advocate for Parental Participation; Manifest Positivity; and Celebrate Milestones. PRACTICE IMPLICATIONS This study identified a wide variety of psychosocial needs for parental caregivers. Results also offered direction for a theory-based hope intervention while highlighting the need for additional research in this area. These results will provide the foundation for a booklet parents can work through in their journey of caring for a child with a life-limiting or life-threatening illness.
Biodiversity of Streptococcus thermophilus Phages in Global Dairy Fermentations

Streptococcus thermophilus strains are among the most widely employed starter cultures in dairy fermentations, second only to those of Lactococcus lactis. The extensive application of this species provides considerable opportunity for the proliferation of its infecting (bacterio)phages. Until recently, dairy streptococcal phages were classified into two groups (cos and pac groups), while more recently, two additional groups have been identified (5093 and 987 groups). This highlights the requirement for consistent monitoring of phage populations in the industry. Here, we report a survey of 35 samples of whey derived from 27 dairy fermentation facilities in ten countries against a panel of S. thermophilus strains. This culminated in the identification of 172 plaque isolates, which were characterized by multiplex PCR, restriction fragment length polymorphism analysis, and host range profiling. Based on this characterisation, 39 distinct isolates representing all four phage groups were selected for genome sequencing. Genetic diversity was observed among the cos isolates and correlations between receptor binding protein phylogeny and host range were also clear within this phage group. The 987 phages isolated within this study shared high levels of sequence similarity, yet displayed reduced levels of similarity to those identified in previous studies, indicating that they are subject to ongoing genetic diversification.
A Game-based Platform to Tackle a Public Health Problem

Mosquito-borne diseases are a global public health concern. Individuals' behavior may affect the transmission dynamics of diseases. For instance, improper storage of water can become a breeding site for the Aedes aegypti mosquito, the vector of dengue, zika, chikungunya and urban yellow fever viruses. Most of the Aedes foci are in or near people's homes and the control of vector population is essential to prevent these diseases. Thereby, population awareness should be part of public health policies. Games are a powerful tool to promote awareness and behavioral changes, including public health. Our research goals are 1) the development and evaluation of a gamification-based system to support entomological and epidemiological surveillance through volunteered contributions, integrated with a 2) a game-based platform to be used as a complementary tool to promote awareness and behavioral changes. Some partial results are the game design, prototypes and demos. The expected results are the release and evaluation of a mixed reality game-based platform to support public health actions based on education and engagement of population aimed at vector control.
Metapop: An individual‐based model for simulating the evolution of tree populations in spatially and temporally heterogeneous landscapes

Metapop is a stochastic individual‐based simulation program. It uses quantitative genetics theory to produce an explicit description of the typical life cycle of monoecious and hermaphroditic plant species. Genome structure, the relationship between genotype and phenotype, and the effects of landscape heterogeneity on each individual can be finely parameterized by the user. Unlike most existing simulation packages, Metapop can simulate phenotypic plasticity, which may have a genetic component, and assortative mating, two important features of tree species. Each simulation is parameterized through text files, and raw data are generated recurrently, describing the allelic state of each quantitative trait locus involved in phenotypic variability. The data can be generated in Genepop or Fstat format, and may thus be analysed with other existing packages. Metapop also automatically computes a range of populations statistics, enabling the user to monitor evolutionary dynamics directly, from gene to metapopulation level.
Roadside Units Placement for Traffic Flows Coverage Requirement in Vehicular Networks

With the development of the vehicular communications technology, roadside units (RSUs) placement problem has recently received considerable attentions in vehicular ad hoc networks (VANETs). This paper addresses the roadside units (RSUs) placement problem for traffic flow coverage requirement number in vehicular ad hoc networks (VANETs). Given a set of road segments, some traffic flows and a coverage requirement number n, the problem is how to find the optimal placement of RSUs, such that each traffic flow is covered by at least n RSUs. We first investigate full RSUs placement problem, which place all the RSUs on the given road segments totally. We prove that this problem is NP-hard. Then, we propose a greedy algorithm to solve the problem and theoretical analysis is given. We also study incremental RSUs placement problem, which place the partial RSUs based on the deployed RSUs on the given road segments. Based on the proposed greedy algorithm, we also propose a algorithm to solve the incremental RSUs placement problem. Extensive simulations have been conducted to show the performance of our proposed algorithms is superior to the other algorithms.
A High Sensitivity Terahertz Imaging System Based on Compressed Sensing

Terahertz superconducting detector has high sensitivity but it's difficult to achieve high-efficiency for large-scale observation with a single detector. To fully improve efficiency and resolution, a high sensitivity terahertz imaging system designed using compressive sensing (CS) principles was proposed in this paper, which uses a single superconducting detector replacing complex and high-cost array detector. With a random selection from linear encoded masks, the proposed imaging system acquired enough incoherent measurements using a 0.85THz superconducting detector. A regularized fr-nurm reconstruction method was employed to reconstruct the imaged scene with fewer measurements compared to the size of the image. Experimental results demonstrated that the proposed imaging system could achieve the improvement of imaging efficiency and resolution.
Pharmacological treatments for functional nausea and functional dyspepsia in children: a systematic review

ABSTRACT Introduction: Chronic idiopathic nausea (CIN) and functional dyspepsia (FD) cause considerable strain on many children’s lives and their families. Areas covered: This study aims to systematically assess the evidence on efficacy and safety of pharmacological treatments for CIN or FD in children. CENTRAL, EMBASE, and Medline were searched for Randomized Controlled Trials (RCTs) investigating pharmacological treatments of CIN and FD in children (4–18 years). Cochrane risk of bias tool was used to assess methodological quality of the included articles. Expert commentary: Three RCTs (256 children with FD, 2–16 years) were included. No studies were found for CIN. All studies showed considerable risk of bias, therefore results should be interpreted with caution. Compared with baseline, successful relief of dyspeptic symptoms was found for omeprazole (53.8%), famotidine (44.4%), ranitidine (43.2%) and cimetidine (21.6%) (p = 0.024). Compared with placebo, famotidine showed benefit in global symptom improvement (OR 11.0; 95% CI 1.6–75.5; p = 0.02). Compared with baseline, mosapride versus pantoprazole reduced global symptoms (p = 0.011; p = 0.009). One study reported no occurrence of adverse events. This systematic review found no evidence to support the use of pharmacological drugs to treat CIN or FD in children. More high-quality clinical trials are needed. Abbreviations: AP-FGID: Abdominal Pain Related Functional Gastrointestinal Disorders; BART: Biofeedback-Assisted Relaxation Training; CIN: Chronic Idiopathic Nausea; COS: Core Outcomes Sets; EPS: Epigastric Pain Syndrome; ESPGHAN: European Society for Pediatric Gastroenterology Hepatology and Nutrition; FAP: Functional Abdominal Pain; FD: Functional Dyspepsia; GERD: Gastroesophageal Reflux Disease; GES: Gastric Electrical Stimulation; H2RAs: H2 Receptor Antagonists; IBS: irritable bowel syndrome; NASPGHAN: North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition; PDS: Postprandial Distress Syndrome; PPIs: Proton Pump Inhibitor; PROMs: Patient Reported Outcome Measures; RCTs: Randomized Controlled Trials; SSRIs: selective serotonin reuptake inhibitors; TCAs: tricyclic antidepressants
Differential expression of a stress‐regulated gene Nr4a2 characterizes early‐ and late‐born hippocampal granule cells

Neural progenitors acquire GFAP expression during the perinatal period and continue to generate granule cells (GCs) in the hippocampal dentate gyrus throughout adulthood. Cellular characteristics of GFAP+ progenitor‐derived late‐born GCs in comparison with early‐born GCs remain unknown. Using genetic fate mapping in mice, we show that early‐ and late‐born GCs are concentrated in the outer and inner side of the GC layer, respectively. We then identify that a nuclear orphan receptor Nr4A2 is preferentially expressed by early‐born GCs. Nr4a2 expression is dynamically regulated in response to restraint stress and glucocorticoid levels, indicating that Nr4a2 is a stress‐regulated gene in GCs. Acute stress suppresses but chronic stress conversely induces Nr4a2 expression in GCs. The survival of newly generated GCs is impaired by chronic restraint stress and long‐term stress after middle age decreases the proportion of late‐born GCs in aged mice. Thus, early‐ and late‐born GCs exhibit characteristic anatomical distribution, differential gene expression, and distinct response to environmental stress.
A Modified Mask for Continuous Cardiac Monitoring during Positive Airway Pressure Therapy

A Positive Airway Pressure mask was modified to be able to measure the heart rate of the wearer during Positive Airway Pressure (PAP) therapy. The mask was modified by attaching sensors to measure Electrocardiography (ECG) and Photoplethysmography (PPG) signals, from which a heart rate was extracted. The ECG signal was recorded using stainless steel electrodes positioned on the wearer’s head and neck. The PPG signal was measured from the wearer’s forehead using a reflectance pulse oximeter attached to the mask. The modified device was tested on 19 healthy participants (mean age30.5 ± 9 years) in a simulated sleeping environment. For both the ECG and PPG signals, the device was able to correctly identify more than 95% of heartbeats for the majority of the participants. However, it was found that by combining the ECG and PPG heartbeat data, the percentage of heartbeats that could be detected was increased to 99%. The results showed that it may be possible to use this device to monitor sleep apnoea patients during PAP therapy in their homes.
Factor Structure and Construct Validity of the Levenson Self-Report Psychopathy Scale (LSRP): A Replication and Extension in Dutch Nonclinical Participants

Abstract The Levenson Self-Report Psychopathy (LSRP) scale is widely used to assess psychopathic traits in noninstitutionalized samples. Recent studies suggest that a three-factor structure measuring Egocentricity, Callousness, and Antisocial factors outperformed the original two-factor structure of the LSRP. This study replicated and extended these findings by examining the factor structure and construct validity of a Dutch version of the LSRP in a community sample (N = 856, subsamples ranging between 140 and 572 participants). Confirmatory factor analysis results corroborated the superiority of the three-factor model of the LSRP, using 19 of the 26 LSRP items. Limitations included the need to specify correlated residuals for some indicators, although these were largely in line with prior studies. Across three subsamples, we found evidence for construct validity of the LSRP subscales. Egocentricity and Antisocial showed a pattern of differential associations with external correlates in accordance with theoretical expectations. Callousness shared some correlates with Egocentricity, others with Antisocial, and uniquely predicted low morality and high physical aggression. Few exceptions to the hypothesized associations were observed, mostly concerning Callousness. Overall, the LSRP three-factor model received further support in a Dutch sample, and is thus recommended in future research, possibly adding items to improve the performance of the Callousness factor.
Method for analysis of electrospray for gene transfer and the impact on cell viability of A549 alveolar epithelial like cells

Electrospray is a process based on creation and acceleration of small sized droplets based on electrostatic repulsion. Spraying plasmid containing liquids this process may be used to transfer genes into cells. Within this paper we report on a method for accessing and evaluating the spray modalities using high speed imaging system with a post processing of image data to obtain estimated volume and velocity of emerging droplets first. Second we investigate on the impact of different media on the spray modalities. Third we evaluate the impact of the spray on cell viability and on transfection efficiency of an eGFP plasmid as reporter gene obtained in an in vitro setup on alveolar epithelial like cells (A549).
Description of Haemaphysalis (Alloceraea) Kolonini sp. nov., a new species in subgenus Alloceraea Schulze (Ixodidae: Haemaphysalis) in China

Southeastern Asia has been postulated as probably the original sites of genus Haemaphysalis, where numerous structurally advanced haemaphysalids now occur. Because of its phylogenetic and biological interest, we report the discovery of a structurally primitive new species Haemaphysalis (A.) kolonini belonging to subgenus Alloceraea from Baoshan and Weixi cities, Yunnan province, southwestern China. Both the morphological and phylogenic characteristics of the species support congruently to erect Hae. (A.) kolonini as a new species. The male and female adults of Haemaphysalis (A.) kolonini are respectively described and illustrated. Taxonomic discussion and keys to Alloceraea species in China are also provided to be able to distinguish species that are close morphologically.