Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects on Edibility of Reused Frying Oils in the Catering Industry

In this study, significant fatty acids, trans fatty acids, peroxide value, and free fatty acids of 25 samples collected from general restaurants, fast-food restaurants, and catering companies in Afyonkarahisar province and neighboring areas were analyzed. Fresh sunflower oil was used as the reference oil. An amount of 50 g of potato pieces was fried at 180°C for 3 min in every frying interval for a total of 15 times of frying in the same oil. Oleic, linoleic, linolenic, trans fatty acids, peroxide value, and free fatty acids of this fresh oil were measured after every frying process and compared to oils collected from different sources. The purpose of this study was to determine the utilization level of frying oils for the restaurant sector to reveal their effect on human nutrition and health as an investigation on recycling oils for the national economy. As a result of the study, free fatty acid levels reached a maximum codex level of 0.6 g/100 g oil at the eighth time frying interval and this level in it reached a 0.3 g/100 g oil codex level at the third time frying. This point was assumed as the start of the rancidity level. A sixth time frying interval gave 10 meq/kg of peroxide value and this point was accepted as the maximum codex level of the Turkish Food Codex, but a hydroperoxide occurence level of 5 meq/kg seemed to be the result of these findings at the third frying interval. It could be said that three times of frying could be done with the same oil, but these oils must be filtered after each use and stored in cold conditions. The results showed that more than half of the companies used these frying oils at least more than three times.
Current challenges and future opportunities of phage therapy.

Antibiotic resistance is a major public health challenge worldwide, whose implications for global health might be devastating if novel antibacterial strategies are not quickly developed. As natural predators of bacteria, (bacterio)phages may play an essential role in escaping such a dreadful future. The rising problem of antibiotic resistance has revived the interest in phage therapy and important developments have been achieved over the last years. But where do we stand today and what can we expect from phage therapy in the future? This is the question we set to answer in this review. Here, we scour the outcomes of human phage therapy clinical trials and case reports and address the major barriers that stand in the way of using phages in clinical settings. We particularly address the potential of phage resistance to hinder phage therapy and discuss future avenues to explore the full capacity of phage therapy.
DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis

Bacillus cereus is an opportunistic pathogen causing food poisoning manifested by diarrhoeal or emetic syndrome. It is closely related to animal and human pathogens Bacillus anthracis and the insect pathogen Bacillus thuringiensis. In the present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were studied. Bacillus cereus resistant to Penicillin (10 units/ml) and Amoxycillin (10 µg/ml); sensitive to Cobalt and Mercury even at 1 mM concentration were determined. All the isolate had plasmid ranging in number from 3-5 and their molecular weight ranged from 3.0 kb-2.0 kb. Plasmid DNA band specific to 6,557 bp has been found in Bacillus cereus isolated from sewage water, air, fresh water, sea water and milk.
The Relationship between Emotionally Laden Landmarks, Spatial Abilities, and Personality Traits: An Exploratory Study

Separate research lines have shown that the way we process spatial information is influenced by individual factors, such as personality traits and basic spatial abilities. Alongside, recent studies suggest that environmental landmarks can be represented differently depending on their emotional content. However, to our knowledge, no study has addressed so far the issue of whether there is a relationship between individual factors and the way we represent and use spatial information that conveys emotional content. Therefore, this exploratory study aimed to (i) investigate the relationship between personality traits and the use of spatial strategies in relation to emotional stimuli; (ii) investigate if a different pattern emerges according to a body- or object-based spatial encodings. After watching movies of routes characterized by positive, negative, or neutral landmarks, participants performed a “route continuation” (RC, i.e., left/right decision) and a “distance comparison” task (DC, i.e., what was the landmark closest to X? ). Furthermore, participants performed a mental rotation task (MR), the Corsi block tapping (CBT), and the Bergen right-left discrimination tests (B-RL). Personality traits were assessed through the Ten Item Personality Inventory (TIPI). Results showed that a better performance at the RC task was associated with higher scores at CBT tasks in the positive condition and at B-RL test and agreeableness scale from TIPI in both positive and neutral conditions. Instead, the MR task positively correlated with the DC task in all conditions. In sum, individuals’ spatial abilities, personality traits, and task requests influenced the way emotionally laden landmarks were memorized.
Daily Morning Blue Light Therapy Improves Daytime Sleepiness, Sleep Quality, and Quality of Life Following a Mild Traumatic Brain Injury

Objective: Identify the treatment effects of 6 weeks of daily 30-minute sessions of morning blue light therapy compared with placebo amber light therapy in the treatment of sleep disruption following mild traumatic brain injury. Design: Placebo-controlled randomized trial. Participants: Adults aged 18 to 45 years with a mild traumatic brain injury within the past 18 months (n = 35). Main Outcome Measures: Epworth Sleepiness Scale, Pittsburgh Sleep Quality Index, Beck Depression Inventory II, Rivermead Post-concussion Symptom Questionnaire, Functional Outcomes of Sleep Questionnaire, and actigraphy-derived sleep measures. Results: Following treatment, moderate to large improvements were observed with individuals in the blue light therapy group reporting lower Epworth Sleepiness Scale (Hedges' g = 0.882), Beck Depression Inventory II (g = 0.684), Rivermead Post-concussion Symptom Questionnaire chronic (g = 0.611), and somatic (g = 0.597) symptoms, and experiencing lower normalized wake after sleep onset (g = 0.667) than those in the amber light therapy group. In addition, individuals in the blue light therapy group experienced greater total sleep time (g = 0.529) and reported improved Functional Outcomes of Sleep Questionnaire scores (g = 0.929) than those in the amber light therapy group. Conclusion: Daytime sleepiness, fatigue, and sleep disruption are common following a mild traumatic brain injury. These findings further substantiate blue light therapy as a promising nonpharmacological approach to improve these sleep-related complaints with the added benefit of improved postconcussion symptoms and depression severity.
Presence and Health Risks of Obsolete and Emerging Pesticides in Paddy Rice and Soil from Thailand and China

Organochlorine (OCPs) and organophosphorus pesticides (OPPs) have been intensively applied in rice paddy field farming to control pest infestation and increase the yield. In this study, we investigated the presence of organochlorine and organophosphorus pesticides in paddy rice and soil from rice plantations in Thailand and China. According to concentration and distribution of OCPs, the most abundant OCPs residues in rice and soil from Thailand and China were dichlorodiphenyltrichloroethane and hexachlorocyclohexanes. The OPPs of methidathion, carbophenothion, chlorpyrifos, and diazinon were common to Thailand and China in both types of samples. The detection frequency of multiple types of these pesticides was greater than 50% of total samples. The relative concentration of some OPPs residues in rice and soil from Thailand and China were significantly different from each other (p < 0.0083), whereas, no significant difference was observed for the relative concentration of OCPs residues in rice and soil from both countries, except for HCHs (p < 0.05). Bioaccumulation factors of OCPs between rice and soil samples indicated that OCPs and OPPs in soil could accumulate in rice. The carcinogenic and non- carcinogenic risks of OCPs and OPPs seem to be in the safe range as recommended by the European Union.
Is character weighting a Panacea for the problem of data heterogeneity in phylogenetic analysis

We recently advocated a philosophical framework for the treatment of potentially diverse data in phylogenetic analysis (Bull et al., 1993). In broad outline, this method involves evaluating whether two or more data sets (partitions of the total available data) provide strongly conflicting esti? mates of the phylogenetic relationships for a group of taxa. We suggested that parti? tioned data should be combined without
From infancy to adulthood—Developmental changes in pulmonary quantitative computed tomography parameters

Purpose Quantified computed tomography (qCT) is known for correlations with airflow obstruction and fibrotic changes of the lung. However, as qCT studies often focus on diseased and elderly subjects, current literature lacks physiological qCT values during body development. We evaluated chest CT examinations of a healthy cohort, reaching from infancy to adulthood, to determine physiological qCT values and changes during body development. Method Dose-optimized chest CT examinations performed over the last 3 years using a dual-source CT were retrospectively analysed. Exclusion criteria were age >30 years and any known or newly diagnosed lung pathology. Lung volume, mean lung density, full-width-at-half-maximum and low attenuated volume (LAV) were semi-automated quantified in 151 patients. qCT values between different age groups as well as unenhanced (Group 1) and contrast-enhanced (Group 2) protocols were compared. Models for projection of age-dependant changes in qCT values were fitted. Results Significant differences in qCT parameters were found between the age groups from 0 to 15 years (p < 0.05). All parameters except LAV merge into a plateau level above this age as shown by polynomial models (r2 between 0.85 and 0.67). In group 2, this plateau phase is shifted back around five years. Except for the volume, significant differences in all qCT values were found between group 1 and 2 (p < 0.01). Conclusion qCT parameters underly a specific age-dependant dynamic. Except for LAV, qCT parameters reach a plateau around adolescence. Contrast-enhanced protocols seem to shift this plateau backwards.
Cost-effective analysis of childhood malaria vaccination in endemic hotspots of Bangladesh

Introduction Bangladesh has a history of endemic malaria transmission, with 17.5 million people at risk. The objective of this study was to assess the cost-effectiveness of universal childhood malaria vaccination in Chittagong Hill Tracts (CHT) of Bangladesh with newly developed RTS,S/AS01 malaria vaccines. Methods A decision model was been developed using Microsoft® Excel to examine the potential impact of future vaccination in Bangladesh. We estimated the economic and health burden due to malaria and the cost-effectiveness of malaria vaccination from the health system and societal perspective. The primary outcomes include the incremental cost per Disability-Adjusted Life Year (DALY) averted, incremental cost per case averted, and the incremental cost per death averted. Results Introducing childhood malaria vaccination in CHT in Bangladesh for a single birth cohort could prevent approximately 500 malaria cases and at least 30 deaths from malaria during the first year of vaccination. The cost per DALY averted of introducing the malaria vaccine compared to status quo is US$ 2,629 and US$ 2,583 from the health system and societal perspective, respectively. Conclusions Introduction of malaria vaccination in CHT region is estimated to be a cost-effective preventive intervention and would offer substantial future benefits particularly for young children vaccinated today. Policies should, thus, consider the operational advantages of targeting these populations, particularly in the CHT area, with the vaccine along with other malaria control initiatives.
Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais

Insecticide resistance patterns among 16 Brazilian populations of the maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), were recognized by surveying resistance to three organophosphates (chlorpyrifos‐methyl, malathion, and pirimiphos‐methyl) and three pyrethroids (cypermethrin, deltamethrin, and permethrin). Two population clusters were obtained: one with three populations (Bragança Paulista, Cristalina, and Nova Andradina) showing low frequency of cypermethrin resistance (13–36%) and negligible frequency of deltamethrin resistance (2–9%); and another with six populations (Campos dos Goytacazes, Ivinhema, Patos de Minas, Penápolis, Uberlândia, and Venda Nova) showing low to negligible levels of pyrethroid resistance (0–23%). The remaining seven populations, including a susceptible, and a DDT‐ and pyrethroid‐resistant reference populations (Sete Lagoas and Jacarezinho, respectively), were significantly different from each other and from the two recognized clusters. In contrast with pyrethroid resistance, organophosphate resistance was negligible except for chlorpyrifos‐methyl in two populations (Fátima do Sul and Penápolis). There was no correlation between geographic distance and the Mahalanobis distance estimated from the resistance pattern ordination of the populations by canonical variate analysis, suggesting local selection and/or broad dispersal of resistant populations by grain trade. The results of biochemical in vitro studies measuring the activity of detoxification enzymes (esterases and glutathion S‐transferases) in conjunction with canonical correlation analysis suggest a major involvement of enhanced conjugation by glutathione S‐transferases (> 2‐fold increase) in pyrethroid resistance and, in the case of cypermethrin resistance, enhanced phosphotriesterase activity.
Evidence for Common Horizontal Transmission of Wolbachia among Ants and Ant Crickets: Kleptoparasitism Added to the List

While Wolbachia, an intracellular bacterial symbiont, is primarily transmitted maternally in arthropods, horizontal transmission between species has been commonly documented. We examined kleptoparasitism as a potential mechanism for Wolbachia horizontal transmission, using ant crickets and their host ants as the model system. We compared prevalence and diversity of Wolbachia across multiple ant cricket species with different degrees of host specificity/integration level. Our analyses revealed at least three cases of inter-ordinal Wolbachia transfer among ant and ant crickets, and also showed that ant cricket species with high host-integration and host-specificity tend to harbor a higher Wolbachia prevalence and diversity than other types of ant crickets. This study provides empirical evidence that distribution of Wolbachia across ant crickets is largely attributable to horizontal transmission, but also elucidates the role of intimate ecological association in successful Wolbachia horizontal transmission.
Negative Capacitance Vacuum Channel Transistors for Low Operating Voltage

This study proposes negative capacitance vacuum channel transistors. The proposed negative capacitance vacuum channel transistors in which a ferroelectric capacitor is connected in series to the gate of the vacuum channel transistors have the following two advantages: first, adding a ferroelectric capacitor in series with a gate capacitor makes the turn-on voltage lower and on–off transition steeper without causing hysteresis effects. Second, the capacitance matching between a ferroelectric capacitor and a vacuum channel transistor becomes simplified because the capacitance of a vacuum channel transistor as seen from a ferroelectric capacitor is constant.
Characterization of Ionic Liquid Lignins Isolated from Spruce Wood with 1-Butyl-3-methylimidazolium Acetate and Methyl Sulfate and Their Binary Mixtures with DMSO

Ionic liquids (ILs) based on 1-butyl-3-methylimidazolium (bmim) cation have proved to be promising solvents for the fractionation of plant biomass with the production of cellulose and lignin. This study deals with the characterization of lignins isolated from coniferous (spruce) wood using [bmim]OAc and [bmim]MeSO4 ionic liquids and their binary mixtures with DMSO (80:20). Molecular weight distributions, functional composition, and structural features of IL lignins were studied by size-exclusion chromatography, NMR spectroscopy (1D and 2D) and atmospheric pressure photoionization high-resolution mass spectrometry. It was shown that the interaction of ILs with lignin leads to significant chemical changes in the biopolymer; a decrease in the degree of polymerization and in the content of free phenolic hydroxyl groups due to alkylation, the disappearance (in the case of [bmim]OAc) of carbonyl groups and a significant destruction of β-O-4 bonds. The chemical reactions between lignin and 1-butyl-3-methylidazolium cation with covalent binding of ionic liquids or products of their decomposition is evidenced by the presence of a large number of nitrogen-containing oligomers in IL lignins.
Origin of HAV strains responsible for 2016–2017 outbreak among MSM: Viral phylodynamics in Lazio region

In Europe HAV infection occurs mainly among specific risk groups, such as consumers of specific food. Sexual transmission of HAV has been demonstrated, particularly among Men-Who-Have-Sex-With-Men (MSM), causing MSM-specific outbreaksin Europe. Here we report a molecular epidemiologic and phylodynamic analysis on HAV sequences in Lazio (central Italy)to identify genetic background and the phylogenetic relations, and test the HAV infection dynamics during a large outbreak through phylodynamic model.Among all HAV sequences found during 2013–2018 in Lazio, low genetic diversity was observed in HAV population in 2016 and 2017, along with high frequenciesVRD_521_2016and RIVM-HAV16-090, suggesting a large expansion event of viral population. The initial expansion of both VRD_521_2016 and RIVM-HAV16-090 clusters dated back to 2012 (95% HPD:2006–2015). During the2016-2017outbreak in Lazio region, the Re peaked around mid-2016, with a value of 1.73 (95% HPD: 1.03–2.37), consistent with incidence trend of AHA cases in Lazio between 2016 and mid-2017. This study showed the magnitude of HAV outbreak in Lazio during 2016–2017, demonstrating the epidemic continuity to MSM-specific outbreak in Europe. The HAV dataset is available on interactive phylodynamic platform https://nextstrain.org to real-time update of future outbreaks.
Negative regulation of Lyn protein‐tyrosine kinase by c‐Cbl ubiquitin‐protein ligase in FcɛRI‐mediated mast cell activation

Background: Recent studies have demonstrated that c‐Cbl functions as a ubiquitin‐protein ligase toward immune receptors and non‐receptor protein‐tyrosine kinase Syk by facilitating their ubiquitination and subsequent targeting to proteasomes. However, it was not clear whether Src family kinase Lyn is regulated by the Cbl family of ubiquitin‐protein ligases.
Cationic Magnetite Nanoparticles for Increasing siRNA Hybridization Rates

An investigation of the interaction principles of nucleic acids and nanoparticles is a priority for the development of theoretical and methodological approaches to creating bionanocomposite structures, which determines the area and boundaries of biomedical use of developed nanoscale devices. «Nucleic acid—magnetic nanoparticle» type constructs are being developed to carry out the highly efficient detection of pathogens, create express systems for genotyping and sequencing, and detect siRNA. However, the data available on the impact of nanoparticles on the behavior of siRNA are insufficient. In this work, using nanoparticles of two classical oxides of inorganic chemistry (magnetite (Fe3O4) and silica (SiO2) nanoparticles), and widely used gold nanoparticles, we show their effect on the rate of siRNA hybridization. It has been determined that magnetite nanoparticles with a positive charge on the surface increase the rate of siRNA hybridization, while negatively charged magnetite and silica nanoparticles, or positively charged gold nanoparticles, do not affect hybridization rates (HR).
Determinants of Treatment Response in Painful Diabetic Peripheral Neuropathy: A Combined Deep Sensory Phenotyping and Multimodal Brain MRI Study

Painful diabetic peripheral neuropathy (DPN) is difficult to manage, as treatment response is often varied. The primary aim of this study was to examine differences in pain phenotypes between responders and nonresponders to intravenous lidocaine treatment using quantitative sensory testing. The secondary aim was to explore differences in brain structure and functional connectivity with treatment response. Forty-five consecutive patients who received intravenous lidocaine treatment for painful DPN were screened. Twenty-nine patients who met the eligibility criteria (responders, n = 14, and nonresponders, n = 15) and 26 healthy control subjects underwent detailed sensory profiling. Subjects also underwent multimodal brain MRI. A greater proportion of patients with the irritable (IR) nociceptor phenotype were responders to intravenous lidocaine treatment compared with nonresponders. The odds ratio of responding to intravenous lidocaine was 8.67 times greater (95% CI 1.4–53.8) for the IR nociceptor phenotype. Responders to intravenous lidocaine also had significantly greater mean primary somatosensory cortex cortical volume and functional connectivity between the insula cortex and the corticolimbic circuitry. This study provides preliminary evidence for a mechanism-based approach for individualizing therapy in patients with painful DPN.
Health of Youth in Transition in Hong Kong

This study aimed to examine the impact of individual (level of vigorous physical activity (VPA) and frequency of using sports and recreation facilities), interpersonal (perceived social cohesion (PSC)), and neighborhood environmental (availability of sports and recreation facilities) factors on youths’ health in transition in Hong Kong. A sample of 508 individuals aged 17–23 years from all Hong Kong council districts randomly completed validated questionnaires by telephone survey. Of 508,302 individuals with complete data pertaining to address geocoding were selected for further analyses. Overall, more than half of them (56.3%) used sports and recreation facilities once per month or less. Structural equation modeling was used to examine the relationship among the studies’ constructs. The results indicated that the proposed model sufficiently fitted the data (χ2 (24) = 32.23, p < 0.12; CFI = 0.977; SRMR = 0.051; RMSEA = 0.034 (90% CI = 0.000 to 0.061). However, two items of PSC were sequentially removed due to their low standardized factor loadings (<0.3). A structural model was reinserted into data analyses, and the modified model fitted the data well as indicated by fit indices (χ2 (11) = 15.29, p < 0.17; CFI = 0.987; SRMR = 0.054; RMSEA = 0.036 (90% CI = 0.000 to 0.075). Only VPA (β = 0.27, p = 0.0005) and PSC (β = 0.12, p = 0.048) were significantly related to perceived health at an individual level. To promote youth health, the Hong Kong government may work with the business sector, community groups, or education institutions to develop community programs to keep youths active (especially VPA) and to build more cohesive, trustful relationships among youths in the neighborhood.