Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of Body Mass Index and Abdominal Obesity with the Risk of Airflow Obstruction: National Health and Nutrition Examination Survey (NHANES) 2007–2012

Abstract This study aimed to explore the relationship between body mass index (BMI) and abdominal obesity and the risk of airflow obstruction, based on the data from the 2007–2012 National Health and Nutrition Survey (NHANES). Logistic regression was applied to assess the relationships between BMI or abdominal obesity and the risk of airflow obstruction by the fixed ratio method and the lower limit of normal (LLN) method. We further used the restricted cubic splines with 3 knots located at the 5th, 50th, and 95th percentiles of the distribution to evaluate the dose-response relationship. A total of 12,865 individuals aged 20–80 years old were included. In the fixed ratio method, underweight was positively correlated with the risk of airflow obstruction, and overweight and obesity were negatively correlated with the risk of airflow obstruction. In the LLN method, the results were consistent with the fixed ratio method. Abdominal obesity was positively associated with the risk of airflow obstruction only in the fixed ratio method (OR: 1.41, 95% CI: 1.04–1.90). There was an additive interaction between underweight and smoking on airflow obstruction in both methods. Abdominal obesity and smoking had additive interactions in the LLN method. Dose-response analysis indicated that there was a non-linear trend between BMI and the risk of airflow obstruction (P for nonlinearity < 0.01). Our study suggested that underweight and abdominal obesity were associated with the increased risk of airflow obstruction, and overweight and general obesity were associated with the decreased risk of airflow obstruction.
Cytokinin increases vegetative growth period by suppressing florigen expression in rice and maize.

Increasing the vegetative growth period of crops can increase biomass and grain yield. In rice, the concentration of trans-zeatin, an active cytokinin, was high in the leaves during vegetative growth and decreased rapidly upon induction of florigen expression, suggesting that this hormone is involved in the regulation of the vegetative phase. To elucidate whether exogenous cytokinin application influences the length of the vegetative phase, we applied 6-benzylaminopurine (BAP) to rice plants at various developmental stages. Our treatment delayed flowering time by 8 to 9 d when compared with mock-treated rice plants, but only at the transition stage when the flowering signals were produced. Our observations also showed that flowering in the paddy field is delayed by thidiazuron, a stable chemical that mimics the effects of cytokinin.The transcript levels of florigen genes Heading date 3a (Hd3a) and Rice Flowering locus T1 (RFT1) were significantly reduced by the treatment, but the expression of Early heading date 1 (Ehd1), a gene found directly upstream of the florigen genes, was not altered. In maize, similary, BAP treatment increased the vegetative phage by inhibiting the expression of ZCN8, an ortholog of Hd3a. We showed that cytokinin treatment induced the expression of two type-A response regulators (OsRR1 and OsRR2) which interacted with Ehd1, a type-B response regulator. We also observed that cytokinin did not affect flowering time in ehd1 knockout mutants. Our study indicated that cytokinin application increases the vegetative phase duration by delaying the expression of florigen genes in rice and maize by inhibiting Ehd1.
Fraxetin down-regulates polo-like kinase 4 (PLK4) to inhibit proliferation, migration and invasion of prostate cancer cells through the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway

ABSTRACT Fraxetin, a natural product isolated from herb Cortex Fraxini, has been demonstrated to exhibit anti-cancer effects on various cancers. The aim of this work is to investigate the anti-tumor effect of Fraxetin in prostate cancer and the potential mechanisms. In this study, the prostatic epithelial cell RWPE-1 and prostate cancer cell DU145 were exposed to Fraxetin (10, 20, 40, and 80 μM) to detect the changes in cell viability using cell counting kit-8 (CCK-8) assay. Fraxetin (10, 20, and 40 μM) was utilized to treat DU145 cell, then the changes in cell proliferation, apoptosis, migration, and invasion were assessed. Western blot assay was employed to detect the expression of proteins that participate in the above cellular processes as well as Polo-like kinase 4 (PLK4), phosphatidylinositol 3-kinase (PI3K). In addition to 40 μM Fraxetin treatment, DU145 cells were overexpressed with PLK4, and then the above experiments were repeated. Results revealed that Fraxetin markedly decreased DU145 cell viability, but didn’t affect the cell viability of RWPE-1. Fraxetin suppressed cell proliferation, migration, invasion, and induced apoptosis of DU145 cells in a concentration-dependent manner. Furthermore, the expression of PLK4 and phosphorylated PI3K and protein kinase B (Akt) were reduced upon Fraxetin treatment. Finally, PLK4 overexpression significantly reversed all the effects of Fraxetin on DU145 cells. Collectively, Fraxetin acted as a cancer suppressor in prostate cancer through inhibiting PLK4 expression thereby inactivating PI3K/Akt signaling. Graphical Abstract
A Method for Evaluating Coastal Underground Barrier Wall Using Groundwater Tidal Response

Tidal response methods are usually used to estimate the hydraulic parameters of coastal aquifers. In this study an analytical model for aquifer tidal response was used. An existing analytical solution for tidal response of groundwater levels was extended to evaluate a subsurface barrier wall to prevent saltwater penetration in a coastal aquifer. A field feasibility study was conducted at the Komesu Dam, Japan. Groundwater levels were observed at pairs of sites on the seaward and reservoir sides of the wall. Groundwater‐level time series data collected from a reservoir‐side site near a horizontal hollow pipe penetrating the wall contained a visible sinusoidal tidal component, whereas data from another reservoir‐side site did not. Analysis of these observations on the groundwater tidal response derived hydraulic parameters of the barrier wall between the paired observation sites. Although the parameters derived by the used simple formulas seem only approximate or apparent, the difference of the results for the two pairs indicated that the extended tidal response method can be useful for evaluation of the barrier function of the wall.
Size‐adjustable ring‐shape photoacoustic tomography imager in vivo

Photoacoustic tomography (PAT) has become a novel biomedical imaging modality for scientific research and clinical diagnosis. It combines the advantages of spectroscopic optical absorption contrast and acoustic resolution with deep penetration. In this article, an imaging size‐adjustable PAT system is proposed for potential clinical applications such as breast cancer detection and screening, which can adapt to imaging targets with various sizes. Comparing with the conventional PAT setup with a fixed radius ring shape ultrasound transducer (UT) array, the proposed system is more flexible for imaging diverse size targets based on sectorial ultrasound transducer arrays (SUTAs). Four SUTAs form a 128‐channel UT array for photoacoustic detection, where each SUTA has 32 elements. Such four SUTAs are controlled by four stepper motors, respectively, and can change their distribution layout position to adapt for various imaging applications. In this proposed system, the radius of the imaging region of interest (ROI) can be adjusted from 50 to 100 mm, which is much more flexible than the conventional PAT system with a full ring UT array. The simulation experiments using the MATLAB k‐wave toolbox demonstrate the feasibility of the proposed system. To further validate the proposed system, imaging of pencil leads made phantom, ex‐vivo pork breast with indocyanine green (ICG) injected, and in‐vivo human wrist, finger and ankle are conducted to prove its feasibility for potential clinical applications.
From fibre to function: are we accurately representing muscle architecture and performance?

The size and arrangement of fibres play a determinate role in the kinetic and energetic performance of muscles. Extrapolations between fibre architecture and performance underpin our understanding of how muscles function and how they are adapted to power specific motions within and across species. Here we provide a synopsis of how this ‘fibre to function’ paradigm has been applied to understand muscle design, performance and adaptation in animals. Our review highlights the widespread application of the fibre to function paradigm across a diverse breadth of biological disciplines but also reveals a potential and highly prevalent limitation running through past studies. Specifically, we find that quantification of muscle architectural properties is almost universally based on an extremely small number of fibre measurements. Despite the volume of research into muscle properties, across a diverse breadth of research disciplines, the fundamental assumption that a small proportion of fibre measurements can accurately represent the architectural properties of a muscle has never been quantitatively tested. Subsequently, we use a combination of medical imaging, statistical analysis, and physics‐based computer simulation to address this issue for the first time. By combining diffusion tensor imaging (DTI) and deterministic fibre tractography we generated a large number of fibre measurements (>3000) rapidly for individual human lower limb muscles. Through statistical subsampling simulations of these measurements, we demonstrate that analysing a small number of fibres (n < 25) typically used in previous studies may lead to extremely large errors in the characterisation of overall muscle architectural properties such as mean fibre length and physiological cross‐sectional area. Through dynamic musculoskeletal simulations of human walking and jumping, we demonstrate that recovered errors in fibre architecture characterisation have significant implications for quantitative predictions of in‐vivo dynamics and muscle fibre function within a species. Furthermore, by applying data‐subsampling simulations to comparisons of muscle function in humans and chimpanzees, we demonstrate that error magnitudes significantly impact both qualitative and quantitative assessment of muscle specialisation, potentially generating highly erroneous conclusions about the absolute and relative adaption of muscles across species and evolutionary transitions. Our findings have profound implications for how a broad diversity of research fields quantify muscle architecture and interpret muscle function.
Frequency and Correlates of Online Consultations With Doctors or Therapists in Middle-Aged and Older Adults: Nationally Representative Cross-sectional Study

Background A few studies have identified the frequency and correlates of online consultations with doctors or therapists. However, there is a lack of studies using nationally representative data from middle-aged and older adults in Germany. Objective This study aims to determine the frequency and correlates of online consultations with doctors or therapists in Germany. Methods For this study, cross-sectional data were taken from the nationally representative German Ageing Survey (DEAS; n=3067 in the analytical sample; age range 46-98 years). As part of the DEAS, a short survey was conducted between June 8 and July 22, 2020, examining the everyday life and living conditions among these middle-aged and older individuals during the COVID-19 pandemic. The frequency of online consultations with doctors or therapists served as the dependent variable (daily, several times a week, once a week, 1-3 times a month, less often, and never). Multiple logistic regressions were performed. Results In sum, 10.02% (381/3806) of individuals with access to the internet had online consultations with doctors or therapists. Multiple logistic regressions showed that the likelihood of using online consultations with doctors or therapists (compared with those never using such services) was positively associated with higher education (compared with medium education; odds ratio [OR] 1.31, 95% CI 1.01-1.70), living with a partner in the same household (compared with single; OR 1.53, 95% CI 1.05-2.22), poorer self-rated health (OR 1.42, 95% CI 1.16-1.74), increased loneliness (OR 1.45, 95% CI 1.10-1.90), and increased satisfaction with life (OR 1.30, 95% CI 1.03-1.64). Conclusions Study findings suggest that a non-negligible proportion of about 1 out of 10 individuals aged 46 years and over had online consultations with doctors or therapists. However, compared with other countries, this proportion remains small. Knowledge about the correlates of (non)use may assist in identifying corresponding individuals. In times of reshaping the health care system, these efforts in online consultations with doctors or therapists may contribute to addressing patient needs. Moreover, increased use of such services may reduce the risk of getting infected with SARS-CoV-2 by reducing social contact.
Complete Genome Sequences of Arthrobacter Phages Eraser, Kaylissa, and Phives

Bacteriophages Phives, Kaylissa, and Eraser are siphoviruses infecting Arthrobacter globiformis B-2880 that were isolated in fall 2019 in Long Island, New York, from soil samples collected in Old Westbury, New York. All three bacteriophages are assigned to phage cluster AZ based on gene content similarity. While many aspects of the genomes are similar across the three phages, the endolysin genes for the phages are different and are located in different locations within the genomes. ABSTRACT Bacteriophages Phives, Kaylissa, and Eraser are siphoviruses infecting Arthrobacter globiformis B-2880 that were isolated in fall 2019 in Long Island, New York, from soil samples collected in Old Westbury, New York. All three bacteriophages are assigned to phage cluster AZ based on gene content similarity. While many aspects of the genomes are similar across the three phages, the endolysin genes for the phages are different and are located in different locations within the genomes.
Distinct Element Modeling of the Shimizu Tunnel No.3 in Japan

In the present research a highway twin tunnel project completed in Japan in 1998 is used as a case study to verify results of numerical analyses with measurement data. Each of the tunnels had approximately 1.1 km of length. For this project a wide geometry of approximately 18.0 m was selected by the designers to facilitate three lanes per tunnel. A sequential tunneling technique known in Japan as the “TBM pilot and enlargement method” was used along with NATM principles. The tunnel was used as a reference project, involving performance testing and extensive monitoring in order to verify and standardize support requirements for other tunnels excavated under similar geologic conditions in the Tomei II expressway. The tunnel was excavated in a region consisting mainly of soft sedimentary rocks, such as locally weathered sandstone, underlain by interbedded sandstone and mudstone. Due to observed non symmetric deformations and loads in the tunnel, the distinct element and the convergence-confinement methods were used during the numerical simulations. A parametric analysis was performed initially in a pseudo-continuum approach to study the behavior of the wide tunnel geometry under various conditions. The effects of rock mass elastic modulus, in situ Ko ratio and boundary conditions are discussed. More complex parametric studies were performed in a stochastically generated model by using joint spatial data from geotechnical investigations. The Barton-Bandis constitutive law was assumed for the behavior of the joints. The sensitivity of the ground “characteristic curves” was examined by statistical variation of the joint shear strength parameters. A final simulation using the code UDEC and the convergence-confinement method yields interesting results which are comparable to the monitored data.
Cell Trafficking at the Intersection of the Tumor–Immune Compartments

Migration is an essential cellular process that regulates human organ development and homeostasis as well as disease initiation and progression. In cancer, immune and tumor cell migration is strongly associated with immune cell infiltration, immune escape, and tumor cell metastasis, which ultimately account for more than 90% of cancer deaths. The biophysics and molecular regulation of the migration of cancer and immune cells have been extensively studied separately. However, accumulating evidence indicates that, in the tumor microenvironment, the motilities of immune and cancer cells are highly interdependent via secreted factors such as cytokines and chemokines. Tumor and immune cells constantly express these soluble factors, which produce a tightly intertwined regulatory network for these cells’ respective migration. A mechanistic understanding of the reciprocal regulation of soluble factor–mediated cell migration can provide critical information for the development of new biomarkers of tumor progression and of tumor response to immuno-oncological treatments. We review the biophysical and biomolecular basis for the migration of immune and tumor cells and their associated reciprocal regulatory network. We also describe ongoing attempts to translate this knowledge into the clinic.
Preliminary mechanism in fetal alloimmune thrombocytopenia associated with anti‐HPA 15b antibodies

Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a bleeding disease that can cause fetal hydrops, a rare but life‐threatening condition in which abnormal amounts of fluid accumulate in one or two areas of the fetus's body. A case of FNAIT with fetal hydrops caused by anti‐HPA‐15b antibodies was involved in this study, as we investigated whether or not anti‐HPA‐15b antibodies can induce endothelial angiogenesis and apoptosis.
Population PBPK modeling using parametric and nonparametric methods of the Simcyp Simulator, and Bayesian samplers

Physiologically‐based pharmacokinetic (PBPK) models usually include a large number of parameters whose values are obtained using in vitro to in vivo extrapolation. However, such extrapolations can be uncertain and may benefit from inclusion of evidence from clinical observations via parametric inference. When clinical interindividual variability is high, or the data sparse, it is essential to use a population pharmacokinetics inferential framework to estimate unknown or uncertain parameters. Several approaches are available for that purpose, but their relative advantages for PBPK modeling are unclear. We compare the results obtained using a minimal PBPK model of a canonical theophylline dataset with quasi‐random parametric expectation maximization (QRPEM), nonparametric adaptive grid estimation (NPAG), Bayesian Metropolis‐Hastings (MH), and Hamiltonian Markov Chain Monte Carlo sampling. QRPEM and NPAG gave consistent population and individual parameter estimates, mostly agreeing with Bayesian estimates. MH simulations ran faster than the others methods, which together had similar performance.
Identifying barriers and facilitators in the development and implementation of government-led food environment policies: a systematic review

Abstract Context Policy-specific actions to improve food environments will support healthy population diets. Objective To identify cited barriers and facilitators to food environment policy (FEP) processes reported in the literature, exploring these according to the nature of the policy (voluntary or mandatory) and country development status. Data sources A systematic search was conducted of 10 academic and 7 grey-literature databases, national websites, and manual searches of publication references. Data extraction Data on government-led FEPs, barriers, and facilitators from key informants were collected. Data synthesis The constant-comparison approach generated core themes for barriers and facilitators. The appraisal tool developed by Hawker et al. was adopted to determine the quality of qualitative and quantitative studies. Results A total of 142 eligible studies were identified. Industry resistance or disincentive was the most cited barrier in policy development. Technical challenges were most frequently a barrier for policy implementation. Frequently cited facilitators included resource availability or maximization, strategies in policy process, and stakeholder partnership or support. Conclusions The findings from this study will strategically inform health-reform stakeholders about key elements of public health policy processes. More evidence is required from countries with human development indices ranging from low to high and on voluntary policies. Systematic Review Registration PROSPERO registration no. CRD42018115034.
Comparison of Cardiovascular Disease Risk Factors Between 2 Subclinical Atherosclerosis Measures in Healthy Postmenopausal Women

Although carotid artery intima media thickness (CIMT) is a widely used determinant of subclinical atherosclerosis, gray‐scale median of the intima‐media complex (IM‐GSM) of the common carotid artery is a relatively novel measure of echogenicity reflecting composition of the arterial wall. It is important to compare cardiovascular disease (CVD) risk factor correlates across CIMT and IM‐GSM to determine whether these measures reflect distinct aspects of atherosclerosis.
Efficacy of intradermal hyaluronic acid plus polynucleotides in vulvovaginal atrophy: a pilot study

Abstract Objective This study aimed to evaluate the safety and efficacy of vulvovaginal intradermal injections of polynucleotides (PN) combined with hyaluronic acid (HA) in postmenopausal women affected by vulvovaginal atrophy (VVA). Materials and methods Postmenopausal women affected by VVA were treated with vulvar and vaginal intradermal injections of one prefilled syringe of 2 ml PN/HA every 2 weeks for four sessions. Patients were evaluated at T0 (baseline), T1 (after session 4) and T2 (1 month after session 4). Evaluation of the treatment was assessed by three international validated questionnaires: Vaginal Health Index (VHI), Vulvar Health Index (VuHI) and Female Sexual Function Index (FSFI). The Wilcoxon matched-paired signed-rank test was used to compare the differences in VHI, VuHI, FSFI and FSFI domains within the groups. Statistical significance was set at p < 0.05. Results Fifty patients were included in the study (mean age 59.9 ± 7.6 years). Overall, the VHI, VuHI and FSFI reported statistically significant differences between baseline and T1 (p < 0.001) and between baseline and T2 (p < 0.001). All FSFI domains registered a statistically significant increase between baseline and T2 (p < 0.001). No complications or side effects were observed. Conclusions Vulvovaginal intradermal injection of PN/HA is a safe, effective treatment, is not expensive and is a reproducible procedure in postmenopausal women with VVA.