Miracle-dz/UniFAR-FTUs huggingface dataset:
Apports de l'échange hydrogène/deutérium couplé à la spectrométrie de masse en protéomique structurale pour la caractérisation de complexes multi-protéiques.

Ce travail de these porte sur developpement de methodes en spectrometrie de masse structurale pour l’analyse de proteines recombinantes et de leurs complexes associes. L’objectif central s’est porte sur des developpements methodologiques en echange hydrogene/deuterium couple a la spectrometrie de masse (HDX-MS). Les techniques biophysiques de caracterisation structurale a haute resolution comme la cristallographie ou la RMN se heurtent regulierement a des problemes de productions de cristaux, de taille de complexes analysables ou encore de quantite de materiel necessaire importante. Le developpement de methodes specifiques HDX-MS a permis de realiser une caracterisation structurale de systemes proteiques varies, et refractaires aux approches haute resolution. La combinaison de cette approche a differents outils de MS structurale est aussi illustree, et montre tout son interet pour l’obtention d’informations a resolution augmentee.
Stress Relief Effectiveness of Visual Elements Presented Through Videos

Stress can negatively impact both the academic aspect and the social aspect of high school students’ life[1]. Although many schools recognize this problem and have employed counselors to help students deal with stress, students seldom contact the counselors because students prefer to deal with the problem alone [2], which rarely yields results. This study aims to test the effectiveness of visual elements such as general movement, rhythm, and texture of the object presented through videos when it comes to stress relief in high school students and tries to explore the therapeutic value of stress relief videos. A 1-minute video showing a device simulating a balloon being repeatedly inflated and deflated while timed to a 4/4 beat was created using 3DS Max and later distributed to participants via WeChat along with a PSS [3] translated by the researcher and a questionnaire with open-ended questions that ask for feedback on the video. The difference in PSS results and the response to the questionnaire suggest that the visual cue of movement affected the perception of stress the most and 70.00% of participants feel relaxed after watching the video.
Production and characterization of semi-quinolone antibiotic produced by Streptomyces griseorubens

This work is an attempt to overcome antimicrobial resistance problem which dispersed worldwide inparticular developing countries due to misuse of antibiotics. Actinobacteria were isolated and screenedagainst selected resistant Gram-negative bacteria to detect the powerful antibacterial activity.Identification of the most potent actinobacterial isolate has been carried out using classical and geneticalmethods. Antibacterial compound has been extracted, purified and characterized using accurate and morespecific techniques and instruments. Among forty actinobacterial isolates, only twenty-two isolates couldinhibit the growth of Gram-negative bacteria. The most potent isolate Eg-7 was identified as S.griseorubens, which has a typical 16S rRNA gene. The antibacterial compound was extracted using ethylacetate, and separated by High Performance Liquid Chromatography using methanol and water as amobile phase. Five active peaks were displayed and retained in the range 40 – 45 min, but the last threepeaks were retained at 41.90, 43.43, and 44.54 min, respectively. The crude extract was analyzed byliquid chromatography mass spectrum, where the active peak was displayed at 721.325 m/z. Theantibacterial compound was purified using flash column chromatography and gel filtration columnchromatography. The active fraction was analyzed by Infra-Red spectrum, where a broad absorption at3338 cm-1 was displayed. Molecular formula of an antibacterial compound was determined by massspectrum as C35H26N6O4. Nuclear magnetic resonance analysis was carried out for an antibacterialcompound. These results suggest that a new antibacterial compound that similar quinolone could beproduced by S. griseorubens and exhibited a higher activity against Gram-negative bacteria.
Analysis of the Geomagnetic Component Z Daily
Variation Amplitude Based on the China Geomagnetic
Network

Abstract. The daily variation amplitude of geomagnetic component Z is one of the important data products in Geomagnetic Network of China (GNC). It comes from the difference between maximum and minimum of the component Z recorded by the geomagnetic instrument in a day. Based on this data product, the daily variation amplitude of Z is analyzed in the past twelve years (2008–2019), including variation for each month in high and low solar activity years, seasonal variations and comparisons between the stations in Yunnan Province and in southeast China. The study indicates that the ionospheric conductivity mainly contributes to the Z daily variations amplitude in the same month or season changing along with solar activity. But the neutral wind in ionosphere could make the Z daily variations amplitude in equinox months equal to or greater than it in summer solstice months during some solar high activity years. Due to the complicated underground electrical structures in Yunnan province, the conductivity underground acts as an amplifier to make the Z daily variations amplitude increase by about 12 % ~ 41 % in Yunnan Province during equinox and summer solstice months.
NAMED ORGANIC REACTIONS (A-D)

Abderhalden ninhydrin reaction Ruhemann, S. J. Chem. Soc. 1910, 97, 2025 Ruhemann, S. J. Chem. Soc. 1911, 99, 792; 1486 Abderhalden, E. ; Schmidt, H. Z. Physiol. Chem. 1911, 72, 37 Abderhalden, E. ; Schmidt, H. Z. Physiol. Chem. 1913, 85, 143 Abderhalden, E. ; Andryewsky, P.Muench. Med. Wschr. 1913, 60, 1641 Abderhalden, E. ; Weil, A. Muench. Med. Wschr. 1913, 60, 1703 Abderhalden, E. Z. Physiol. Chem. 1913, 81, 90 Abderhalden, E.Muench. Med. Wschr. 1913, 59, 2172 Abderhalden, E.Muench. Med. Wschr. 1913, 60, 2774 Abderhalden, E. Deutsche Med. Wschr. 1913, 38, 2160 Abderhalden, E. Deutsche Med. Wschr. 1914, 40, 428 Abderhalden, E.Muench. Med. Wschr. 1914, 61, 233; 546 Abderhalden, E. ; Fodar, A. Muench. Med. Wschr. 1914, 61, 765 Abderhalden, E. ; Holle, H. ; Strauss, H.Muench. Med. Wschr. 1914, 61, 804 Abderhalden, E. ; Paquin, M. Muench. Med. Wschr. 1914, 61, 806 Abderhalden, E. Fermentforschung 1921, 4, 338 Abderhalden, E. Fermentforschung 1922, 5, 84;119;130;163; 342 Abderhalden, E. ; Wertheimer, E. Fermentforschung 1923, 7, 106 Abderhalden, E. Fermentforschung 1927, 9, 119 Abderhalden, E. ; Haas, R. Fermentforschung 1927, 9, 132 Abderhalden, E. Fermentforschung 1940-1941, 16, 297; 327 Abderhalden, E. Fermentforschung 1942, 16, 414
Enabling Native Coexistence Between ICN and TCP/IP Architectures Over the Same Domain

Information-Centric Networking (ICN) has emerged as an alternative to overcome some issues imposed by TCP/IP architecture, such as the lackof mobility, security, and Quality-of-Service (QoS) native support. Seeing asTCP/IP will not disappear anytime soon, given the size of today’s Internet, mechanisms to coexist multiples ICN architectures along with TCP/IP are neces-sary. Through concepts like Network Function Virtualization (NFV), Software-Defined Networking (SDN) and Data Plane Programmability (DPP), we propose in this paper the FIACS, a system capable of allowing pairs of entitiesto communicate transparently with each other by using its L2 access link andnative protocols stacks over the same underline infrastructure.
Application of modified Logistic and Monod models in a single equations system framework to improve cell culture growth modeling and estimation

In modeling cell culture growth using unstructured model, two types of equations are normally used: logistic and Monod. However, these two equations are known for their limitations to model death phase of cell culture growth and to account for dead cells accumulation data. In this paper, we present a modeling framework whereby both Logistic and Monod equations can be used in a single set of equations system to overcome these limitations. First, it can be shown that the increase of total cell population that consists of viable and dead cells follows a logistic growth pattern with its own intrinsic growth rate and total carrying capacity. Furthermore, a hybrid Logistic-Monod equation with first-order decay kinetics can be used to model viable cell growth data with decline phase effectively. With this paradigm, a pseudo-rate equation can be written to account for dead cells accumulation data using population balancing with a simple understanding that dead cell population is simply the difference between total and viable cells. These equations can be adjoined with substrate consumption and product generation rate equations to depict complete batch growth data that covers exponential growth and death phases. This modeling framework has been fitted successfully to fit batch growth data of two cell lines from published literature with complete depictions of dead cell accumulation and cell viability profiles. The implication of this modeling framework for chemostat culture performance analysis is further investigated.
A Propensity Score Analysis of the Effect of a Single Dose Vitamin A Supplementation on Child Hemoglobin Status in Bangladesh

ABSTRACT Vitamin A deficiency (VAD) may cause anemia. The research aimed at evaluating the effect of Vitamin A supplementation (VAS) on hemoglobin (Hb) is, however, limited and, conclusions drawn from a few studies have been concluded inconsistently. In Bangladesh, the prevalence of childhood anemia is high, as well as the prevalence of VAD. Therefore, the purpose of this study was to assess the effect of single high dose VAS on Hb of 6–59 months aged children in Bangladesh. A total of 2,058 children was extracted from the 2011 Bangladesh Demographic Health Survey (BDHS), who received a single dose of VAS in the preceding 6 months. Propensity score matching (PSM) was used by carefully incorporating the survey design to make causal inferences based on this nationally representative survey data. Results show that in sociodemographic variables, VA supplemented and non-supplemented groups were homogeneous. According to PSM, VAS was not significantly related to child Hb status. Stratified analysis based on household wealth status indicates that wealth status was not changing this relationship. Single high dose of VAS alone was not improved child Hb status in Bangladesh. Accordingly, there is a need to strengthen the food fortification program, enhance dietary diversity, increase nutritional education and awareness. Furthermore, future research is needed to comprehend the complex pathways.
Reconhecimento de Padrões de Falhas Tipo Fenda em Chapa de Alumínio Utilizando Clusterização Fuzzy

Pedro Henrique S. Coutinho, Thiago P. das Chagas, Fábio da C. Cruz DCET UESC Ilhéus, Bahia, Brasil Emails: coutinho.p92@gmail.com, tpchagas@uesc.br, fabiocruz28@yahoo.com. br Eduardo F. Simas Filho PPGEE UFBA Salvador, Bahia, Brasil Email: eduardo.simas@ufba.br Maria C. S. Albuquerque, Ivan C. Silva, Cláudia T. T. Farias LEUS IFBA Salvador, Bahia, Brasil Emails: cleaalbuquerque@ifba.edu. br, ivansilva@ifba.edu. br, cfarias@ifba.edu. br
Design and Validation of the Brief Self Online Scale (SO-8) in Early Adolescence: An Exploratory Study

For the Z-Generation, the Internet has become a very important experimentation laboratory for the discovery and validation of their identity. Despite the importance of the process of building the self in the adolescent, there are hardly any validated instruments that measure the self online. The aim of this research was to design and validate the Brief Self Online Scale (SO-8). A total of 843 students (384 boys, 45.6%), with an age range of 10 to 14 years participated. Confirmatory factor analysis (CFA) confirmed the hypothesized model of two correlated factors (Online Self-Perception and Online Idealized Projection), previously obtained through exploratory factor analysis (EFA). The reliability coefficients of Self Online dimensions were adequate. Indicators of convergent validity were obtained, finding significant correlations with self-concept, problematic Internet use, and online emotional intelligence. The SO-8 has adequate psychometric properties to be considered a reliable and valid tool to measure the construct of the Self Online in adolescents.
Theatre as a teaching resource to work on socio-environmental problems in primary education

The theatre as a didactic resource in the educational field has a great pedagogical value (Cutillas-Sánchez, 2006). Unlike in other European and Latin American countries, in Spain it has not had a great protagonist (Baldwin, 2014; De Diego, 2004). However, in recent years in Spain there are various experiences that are taking place in educational classrooms where the theatre is used as a teaching and learning tool, highlighting its usefulness to promote the teaching-learning processes. Therefore, and based on the research conducted, we can affirm that the benefits of this resource at the educational level are related to educational implications referring to both competencies and skills, as well as to the teaching-learning processes (table 1).
Brain-electric activity during eyes open Brahma Kumaris Rajayoga meditation.

Brahma Kumaris Rajayoga’s open eyed ‘seed stage meditation’ was studied in 52 meditators. Meditation stages: concentration on peace, realization of being a soul and communion with the Supreme Soul. Frequency domain brain electric source localization was used on multichannel EEG recordings to establish activation differences between meditation and open eyed, task-free resting. Additional exploratory analyses probed for effects of passing time from initial rest through meditation to final rest. Meditation showed reduced activity in delta and increased activity in low alpha frequencies. Affected brain areas comprised the following networks: central executive network, mirroring network, task-positive and task-negative network. These altered activations reflect the main cognitive-affective and behavioral specifics of seed stage meditation: attention modulation, self-related processing, experiencing the soul as a point of light between the eyes, endowing the soul with the properties (peacefulness) of the Supreme Soul. Future studies need to differentiate between the stages of the meditation.
Determination of Structural Carbohydrates and Lignin in Biomass

NREL Laboratory Analytical Procedures for standard biomass analysis are available electronically at DISCLAIMER These Standard Biomass Analytical Methods (" Methods ") are provided by the National Renewable Energy Laboratory (" NREL "), which is operated by the Alliance for Sustainable Energy, LLC (" ASE ") for the Department Of Energy. Access to and use of these Methods shall impose the following obligations on the user. The user is granted the right, without any fee or cost, to use, copy, modify, alter, enhance and distribute these Methods for any purpose whatsoever, except commercial sales, provided that this entire notice appears in all copies of the Methods. Further, the user agrees to credit NREL/ASE in any publications that result from the use of these Methods. The names NREL/ASE, however, may not be used in any advertising or publicity to endorse or promote any products or commercial entity unless specific written permission is obtained from NREL/ASE. The user also understands that NREL/ASE is not obligated to provide the user with any support, consulting, training or assistance of any kind with regard to the use of these Methods or to provide the user with any updates, revisions or new versions.
Addressing Socio-Material Issues for an Emerging Innovation Ecosystem: Insights From Cultural Heritage

This article deals with how innovation ecosystems emerge and become established. It focuses on technologies and how they favor interaction among actors, resources, and practices to facilitate an ecosystem's workability from a long-range perspective. The intrinsically complex and systemic nature of an innovation ecosystem is investigated by looking at the social and material issues concerning the organizing of social interactions, technologies, and other resources. This article adopts an action research approach through the combination of theory and practice and the support of actors—including the authors—involved in the empirical context of the Technological District for Cultural Heritage (DATABENC). This article contribute to the innovation ecosystem literature by assuming that innovation ecosystems emerge from the interplay of deliberate and unforeseen processes. Prompted by technologies, three milestones emerge supporting innovation ecosystem viability: 1) setting the socio-material context; 2) establishing interdependencies among actors, activities, and resources; and 3) giving continuity to social- and material-oriented interactions. In this context, technology is not merely an artefact; it takes three different roles: context builder, mediating mechanism, and institutionalization process enabler.
HIF-1α and Hypoxia Responsive Genes are Differentially Expressed in Leukocytes From Survivors and Non-Survivors Patients During Clinical Sepsis

Supplemental Digital Content is available in the text ABSTRACT Hypoxia inducible factor 1 alpha (HIF-1α) is linked to the metabolic and immune alterations in septic patients. Stabilization of HIF-1α by hypoxia or inflammation promotes the expression of several genes related to glycolytic metabolism, angiogenesis, coagulation, cell proliferation, and apoptosis. Here, we analyzed public available blood transcriptome datasets from septic patients and evaluated by PCR array the expression of HIF-1α and other hypoxia responsive genes in peripheral blood mononuclear cells from patients with sepsis secondary to community acquired infections. Samples were collected at intensive care unit admission (D0, n=29) and after 7 days follow-up (D7, n = 18); healthy volunteers (n = 10) were included as controls. Hypoxia and glycolysis were among the top scored molecular signatures in the transcriptome datasets. PCR array showed that 24 out of 78 analyzed genes were modulated in septic patients compared with healthy volunteers; most of them (23/24) were downregulated at admission. This same pattern was observed in surviving patients, while non-survivors presented more upregulated genes. EGLN1, EGLN2, and HIF1AN, inhibitors of HIF-1α activation were downregulated in patients, regardless of the outcome, while HIF-1α and other target genes, such as PDK1 and HMOX1, expression were higher in non-survivors than in survivors, mainly at D7. Non-survivor patients also presented a higher SOFA score and lower PaO2/FiO2 ratio. Our results indicate a differential modulation of hypoxia pathway in leukocytes between septic patients who survived and those who did not survive with an increased intensity at D7, which is possibly influenced by disease severity and may affect the immune response in sepsis.
Applied Study of Eco-Interior Concept in Lemongrass Restaurant, Bogor

The application of eco-interior to buildings and interiors has become a phenomenon and is required by developing environmental issues. Most restaurants in Indonesia have developed the waste zero management environmental issue. Environmental issues sustainable need to applicable for the interior and building space associated with the division of space. The development of restaurants in Jakarta is very developed and all require applying eco-design concept. Restaurants was established with a variety of that are quite interesting and make visitors feel comfortable in the restaurant. One of them is Lemongrass Restaurant founded in a natural modern restaurant with indoor and outdoor concepts as well as a green open area with a garden and pool concept. As a very important public facility and needs aspects of applied eco-interior. Seen from the whole building starting from architecture, interior, until the parts that are designed together with each other to become an eco-friendly tropical paradise. Certainly, Lemongrass Restaurant combines colors and tropical plants to make it very natural modern. Selection of special design styles to unite the design with the natural surroundings. The design concept of Lemongrass Restaurant is creating atmosphere with a touch of nature that is equipped with a variety of unique and 'eye catching' support sustain systems and materials.
pH-Triggered release of gemcitabine from polymer coated nanodiamonds fabricated by RAFT polymerization and copper free click chemistry

Prodrug (gemcitabine)-based polymer coated nanodiamonds (NDs) were fabricated by grafting onto method and strain-promoted alkyne–azide cycloaddition (SPAAC) click chemistry. The polymers were synthesized by polymerization of a gemcitabine prodrug monomer and oligoethylene glycol methyl ether acrylate (OEGMEA) using a dibenzocyclooctyne (DBCO) containing reversible addition-fragmentation transfer (RAFT) agent. The covalent conjugation between the polymers and surface groups was carried out through an efficient approach by copper free click chemistry. The resulting NDs were found to release the drug in its active form much faster in acidic environment than in physiological condition. The composition of the polymer shell and the coating modality significantly influence the IC50 values of the obtained NDs for AsPC-1 cells. Specifically, with the NDs coated with copolymers containing higher OEGMEA content displaying comparable IC50 as free gemcitabine, whereas NDs coated with lower fraction of OEGMEA result in higher IC50 values.
Quality of Life after Total Parathyroidectomy in Patients with Secondary Hyperparathyroidism

Background. Patients with end-stage renal disease (ESRD) have a decreased quality of life (QoL), which is attributable in part to secondary hyperparathyroidism (SHPT). Surgery is the definitive treatment for SHPT. The aim of this study is to assess the effect of total parathyroidectomy (PTX) with deltoid autotransplantation on QoL.Methods: A total of 201 ESRD patients with SHPT were enrolled. The operation efficacy was evaluated by analyzing preoperative and postoperative values, including the levels of intact parathyroid hormone (PTH), serum phosphorus, serum calcium, alkaline phosphatase (ALP) and calcium-phosphorus product. The QoL was evaluated by scores on the Medical Outcomes Study 36-item short-form (SF-36) health survey preoperatively and 6 months postoperatively.Results: Compared with preoperatively, postoperatively, the levels of iPTH (2033 pg/ml vs 62.5 pg/ml), serum phosphorus (2.30 mg/dl vs 1.60 mg/dl), serum calcium (3.62 mg/dl vs 1.84 mg/dl), and calcium-phosphorus product were all decreased. Variations in the number and anatomic location of thyroid glands were found in the patients. QoL improved significantly in all 8 individual and 2 component summary scales, with a more significant decrease in the physical health scales.Conclusion: PTX significantly improves QoL in patients with SHPT.