Miracle-dz/UniFAR-FTUs huggingface dataset:
Structure and Expression of the Human Small Cytokine B Subfamily Member 11 (SCYB11/formerly SCYB9B, alias I-TAC) Gene Cloned from IFNHuman gamma-Treated Monocytes (THP-1)

Among CXC chemokines, monokine induced by interferon-gamma (IFN-gamma) (MIG) and IGN-gamma-inducible protein, 10 kDa (INP10), constitute a distinct group because of their sequence and function. We studied genomic structure and expression of a third, recently identified member of this group named small inducible cytokine B subfamily member 11 (SCYB11, formerly SCYB9B) or IFN-inducible T cell alpha chemoattractant (I-TAC). The cDNA (1445 bp) for this 94 amino acid protein (Mr 10,364) was cloned from IFN-gamma-treated human myelomonocytic cells (THP-1). The reading frame of SCYB11 is distributed to 4 exons spanning 1197 bp of the genomic sequence. In vitro transcription/translation yielded a single protein of about 10 kDa, indicating that the deduced reading frame is translated by eukaryotic ribosomes. The recombinant 73 amino acid mature protein overexpressed in Escherichia coli was chemotactic for interleukin-2 (IL-2)-selected T memory cells. Studying various cytokines and lipopolysaccharide in THP-1 cells.
Direct Voltage Control of Signaling via P2Y1 and Other Gαq-coupled Receptors*

Emerging evidence suggests that Ca2+ release evoked by certain G-protein-coupled receptors can be voltage-dependent; however, the relative contribution of different components of the signaling cascade to this response remains unclear. Using the electrically inexcitable megakaryocyte as a model system, we demonstrate that inositol 1,4,5-trisphosphate-dependent Ca2+ mobilization stimulated by several agonists acting via Gαq-coupled receptors is potentiated by depolarization and that this effect is most pronounced for ADP. Voltage-dependent Ca2+ release was not induced by direct elevation of inositol 1,4,5-trisphosphate, by agents mimicking diacylglycerol actions, or by activation of phospholipase Cγ-coupled receptors. The response to voltage did not require voltage-gated Ca2+ channels as it persisted in the presence of nifedipine and was only weakly affected by the holding potential. Strong predepolarizations failed to affect the voltage-dependent Ca2+ increase; thus, an alteration of G-protein βγ subunit binding is also not involved. Megakaryocytes from P2Y1-/- mice lacked voltage-dependent Ca2+ release during the application of ADP but retained this response after stimulation of other Gαq-coupled receptors. Although depolarization enhanced Ca2+ mobilization resulting from GTPγS dialysis and to a lesser extent during \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{AlF}_{4}^{-}\) \end{document} or thimerosal, these effects all required the presence of P2Y1 receptors. Taken together, the voltage dependence to Ca2+ release via Gαq-coupled receptors is not due to control of G-proteins or down-stream signals but, rather, can be explained by a voltage sensitivity at the level of the receptor itself. This effect, which is particularly robust for P2Y1 receptors, has wide-spread implications for cell signaling.
Average Age at First Reproduction of the Northern Fur Seal (Callorhinus ursinus)

Three methods of estimating average age at first reproduction of the northern fur seal, Callorhinus ursinus, are presented. For the first, the percentage of females pregnant for the first time is estimated from the percentage of primiparous pregnant females in the population. For the second, the percentage of females pregnant for the first time is estimated from the difference in the percentages of females pregnant at least once at successive ages. The jackknife variances of both estimates are presented. The estimates are calculated for the 1954–64 year-classes of northern fur seals using data collected at sea by the United States and Canada during 1958–74. By fitting parametric curves to the fraction pregnant at least once as a function of age and applying the second method to that resulting curve, the average age at first reproduction is estimated in a third way; this method is applicable to year-classes for which data are incomplete and estimates are given for the 1952, 1953, 1965, and 1966 year-classe.
The influence of diatom abundance on the egg production rate of Calanus helgolandicus in the English Channel

Weekly measurements of Calanus helgolandicus egg production rates and phytoplankton species identification and abundance have been carried out at a station in the English Channel for a period of 7 yr. These data have been analyzed to investigate the effects of the microplankton composition on egg production rates with special emphasis on groups where nutritional value is controversial, such as diatoms and the Prymnesiophyte, Phaeocystis pouchetii. Diatoms did not show any negative effect on the egg production rate. Phaeocystis pouchetii also showed no negative effects, whereas in contrast, a dinoflagellate, Gyrodinium aureolum, clearly showed a negative effect. We conclude that diatoms do not have a negative effect on egg production rates at the concentrations found in the field and for these natural species assemblages, and that when such an effect exists, as is the case with G. aureolum, the effect can be clearly identified.
Vertical Transport, Entrainment, and Scavenging Processes Affecting Trace Gases in a Modeled and Observed SEAC4RS Case Study

The convectively driven transport of soluble trace gases from the lower to the upper troposphere can occur on timescales of less than an hour, and recent studies suggest that microphysical scavenging is the dominant removal process of tropospheric ozone precursors. We examine the processes responsible for vertical transport, entrainment, and scavenging of soluble ozone precursors (formaldehyde and peroxides) for midlatitude convective storms sampled on 2 September 2013 during the Studies of Emissions, Atmospheric Composition, Clouds and Climate Coupling by Regional Surveys (SEAC4RS) study. Cloud‐resolving simulations using the Weather Research and Forecasting with Chemistry model combined with aircraft measurements were performed to understand the effect of entrainment, scavenging efficiency (SE), and ice physics processes on these trace gases. Analysis of the observations revealed that the SEs of formaldehyde (43–53%) and hydrogen peroxide (~80–90%) were consistent between SEAC4RS storms and the severe convection observed during the Deep Convective Clouds and Chemistry Experiment (DC3) campaign. However, methyl hydrogen peroxide SE was generally smaller in the SEAC4RS storms (4%–27%) compared to DC3 convection. Predicted ice retention factors exhibit different values for some species compared to DC3, and we attribute these differences to variations in net precipitation production. The analyses show that much larger production of precipitation between condensation and freezing levels for DC3 severe convection compared to smaller SEAC4RS storms is largely responsible for the lower amount of soluble gases transported to colder temperatures, reducing the amount of soluble gases which eventually interact with cloud ice particles.
Distinction Between the Gram Reaction and the Gram Type of Bacteria

The elimination of the terms “gram positive, negative, and variable” and their replacement by the terms “Gram reaction positive, negative, and variable” and “Gram type positive, negative, and zero” are proposed. This action would permit a distinction between the reporting of the results of the Gram staining reaction and the placing of the bacteria into general taxonomic groups.
Effects of forest harvesting on Bufflehead and Common Loon foraging behavior

ABSTRACT We compared foraging behavior of Bufflehead (Bucephala albeola Linnaeus) and Common Loon (Gavia immer Brünnich) on eight lakes in harvested and unharvested boreal mixedwood forest in northern Alberta, Canada. For one summer before (1996) and two summers after (1997, 1998) forest harvesting around three of the eight lakes, we recorded the duration of Bufflehead and Common Loon dives. After logging, forested buffer strips 100 m-wide separated cut-blocks from lakes (‘harvested lakes’). ‘Unharvested lakes’ were surrounded by ⩾450 m of undisturbed forest throughout the study. There were no detectable differences in dive duration between harvested and unharvested lakes for Bufflehead or Common Loon. Correlations between environmental variables (water clarity, fish biomass, depth) and the duration of Common Loon dives were not significant. However, the duration of Bufflehead dives differed between lakes, unrelated to forest harvesting. The duration of Buffle-head dives was negatively correlated with water clarity but was not significantly correlated with fish biomass. While our study shows that the foraging behavior of Buffle-heads was affected by lake conditions, the utility of aquatic birds as indicators of the effects of forestry on western boreal lakes remains unproven.
Measuring the size of the informal tourism economy in Thailand

This study is the first to estimate the size of the informal tourism economy. Using a dynamic general equilibrium model, this paper first estimates the size of the informal tourism economy and then assesses its linkages to key labour market variables in Thailand. Empirical results indicate that: (a) the informal tourism economy grows faster than the formal tourism and aggregate economy; (b) both formal and informal tourism economies absorb the unemployed; (c) the relationship between formal and informal economies is negative in the aggregate but positive in the tourism sector.
MLAPS: A Machine Learning based Second Line of Defense for Attack Prevention in IoT Network

Intrusion Prevention Systems (IPSs) are processes that are handled as add-ons to the Intrusion Detection Systems (IDSs) in order to effectively resist and prevent invasions in IoT devices. In this paper, a comparative study on various Machine learning (ML) based Intrusion Prevention System (IPS) has been proposed on UNSW-NB15 dataset by applying various ML methods for classification. For Intrusion Prevention System, two level classifiers for dropping of packets are implemented. Level 1 classifier comprises of Decision Tree (DT) whereas Level 2 classifier uses Random Forest (RF). The proposed IPS automatically adapts to system changes and further reduces training time and increases the accuracy of the system and acts as a lightweight Second Line of Defense System for an IoT Network. In this system, threshold value is calculated by finding the average time of execution of all of the data in the training set.
THE POWER OF LANGUAGE IN ORWELL’S NOVEL: ANIMAL FARM A PRAGMATIC STUDY

This research is concerned with analysing pragmatically the power of language upon people. The analysis focuses on the British novel, 'Animal Farm' by George Orwell. This study aims at analysing the language used in the novel and showing how it can be used as an oppressive device that can be manipulated to lead to a totalitarian state. The model of analysis that is used in the current study is Grice’s theory of implicature and the cooperative principles (1975). The researcher employs qualitative method to have deep understanding and examination to the data of the present study. The results of this research reveal that pragmatic analysis shows that language can be used as a tool to spread power and authority. It can lead to a whole totalitarianism when those in power imply their aims and intuitions in the words they use. The implied meaning occurs when the speaker violates the relevance, the manner, the quality and the quantity maxims and being uncooperative. The study also shows the role and power that the language has upon the thought and behaviour of people
Electric field effect on spin waves: Role of magnetic moment current

We show that a static electric field E x gives rise to a shift of the spin wave dispersion relation in the direction of the wave number q y of the quantity . This effect is caused by the magnetic moment current carried by the spin wave itself which generates an additional phase proportional to the electric field, as in the Aharonov-Casher effect. This effect is independent of the possibly present magneto-electric effects of insulating ferromagnets and superimposes to them.
‘I teach them that anything is possible’—exploring how adult leaders perceive and handle social factors of youth mental health in the context of young people’s civic engagement

The aim of this qualitative study was 2-fold: to explore, in the context of young people’s civic engagement in Sweden, (i) how adult leaders perceive social factors of youth mental health and (ii) how adult leaders handle such social factors within their organizations. Interviews were conducted with leaders engaged in various civic organizations that provide leisure activities for young people. Using thematic analysis, three themes were constructed. Firstly, the social landscape of youth mental health described how adult leaders perceived the social factors of youth mental health within the context of civic engagement. Secondly, the organizational structures developed by adult leaders illustrated the organizing forms that leaders created for young people’s civic engagement. Thirdly, adult leaders’ strategies for addressing the social factors of youth mental health reflected the strategies developed to handle e.g. stress and achievement pressure. The adult leaders recognized the importance of their organizations and their huge potential to have a positive impact on youth mental health. However, some participants also saw limitations in terms of their own resources and competence. They found themselves having to address the complex issue of social factors of youth mental health regardless of whether they felt competent and prepared to do this or not. Our findings contribute to the existing knowledge on youth and community development via the role of adult leaders in promoting young people’s mental health by highlighting the organizational structures and leadership strategies developed by them.
No evidence for premenstrual behavioral changes in captive patas monkeys

Les observations preliminaires de singes patas en captivite ne revelent aucune evidence de changement significatif quant aux comportements sociaux ou agonistiques, (a) tout au long du cycle menstruel, (b) plus specifiquement pendant la phase initiale premenstruelle/menstruelle. Malgre une mention de comportements apparentes au PMS (syndrome pre-menstruel) chez les babouins sauvages, il n'existe jusqu'a present aucune preuve suffisante pour justifier l'extension du label PMS aux primates non-humains
Students’ Motivations to Study Abroad: The Case of International Students at the University of Debrecen

Today there is a growing demand among international students to study in Hungary, more specifically with the launching of Stipendium Hungaricum program in 2013. The number of international scholarship holders increases each year, but till now, we have no significant research results about their study motivations, research has focused only on institutional perspectives (Kasza, & Hangyal 2018). The motivation of students is an important research field in higher education, particularly because there could be various factors to motivate students studying abroad. The motives can be categorised as intrinsic and extrinsic. Our research question is, what are the main intrinsic and extrinsic motivational factors that encourage students to study in Hungary? We performed 15 qualitative interviews among international scholarship holders on postgraduate levels. We gathered semi-structured interviews with the help of an interview scheme with five main questions. The investigated dimensions include the following: 1. introduction and context of interviewees, 2. Reasons for leaving the country of origin, 3. Reasons for country choice, 4. Preliminary knowledge about Hungary, 5. Personal expectations. After the data collection, we analysed texts with open coding process in which line by line and word for word was analysed (Khandkar, 2009). Then we were able to form a typology of motivations and we found subcategories within the intrinsic and extrinsic classification.
Cloning and characterization of iaaM and iaaH from Erwinia herbicola pathovar gypsophilae

Erwinia herbicola pv. gypsophilae induces galls on its host, Gypsophila paniculata. A 16-kb DNA fragment derived from a 78-Md native plasmid with homology to the iaa operon of Pseudomonas syringae pv. savastanoi was isolated from an EMBL3 library of E. h. gypsophilae, strain PD713, DNA. A 7.5-kb EcoRI fragment was subcloned into pUC118 to generate pEG101. Escherichia coli DH5a cells transformed with pEG101 produced indole-3-acetic acid (IAA) when cultured in medium supplemented with L-tryptophan (TRP) []
TRADEOFFS BETWEEN ESCAPE BEHAVIOR AND FORAGING OPPORTUNITY BY THE BALEARIC LIZARD (PODARCIS LILFORDI)

Optimal escape theory predicts that prey permit closer approach by predators when fleeing is more costly, but does not predict other aspects of escape such as distance fled or the likelihood of returning to the initial site in the presence or absence of a resource such as food. Because a lizard preparing to feed may lose the feeding opportunity, optimal escape theory predicts that the lizard should allow a predator to approach closer before fleeing when a stationary food source is present than in its absence. In addition, we predicted that when a predator was nearby, lizards would flee a shorter distance and return more often when food was present than absent. We presented adult males of the omnivorous Balearic lizard, Podarcis lilfordi, with a tethered piece of pear or a pebble of similar size and shape. One of us approached a lizard in a standardized manner, stopping and remaining still when the lizard fled. The other investigator recorded escape and return behaviors. Lizards in the presence of food permitted closer approach before fleeing, fled a substantially shorter distance, and were far more likely to return to the site of stimulus presentation than when a pebble was presented. These findings suggest that prey may alter several aspects of escape behavior to reduce costs due to lost opportunities, and present a likelihood that interspecific variation exists in the combination of aspects of antipredatory behavior that are modified.
Conceptualisation of Hotel Guest Experience Assessment Process and Electronic Words of Mouth

The success of a hotel depends on the guests’ interpretation and reviews of their visit experience. Guests who perceived their visit experience as worthiness and satisfactory would circulate their experience to their acquaintances. In today’s information based era, their visit experience can be widely circulated through electronic word of mouth (e-wom) such as the social media. This paper aims to construct a research framework to study hotel guests experience responses as the result from their encounters with the hotel’s products and services during their stay. Unforgettable encounters would influence the guests’ future behaviour, starting with their commitment and eventually translated into reaction in the forms of electronic word of mouth responses. Methodically, an exhaustive review of the literature was carried out to establish a theoretical underpinning of hotel guests experience assessment process and their future responses. Perceived value and satisfaction are deemed as important measurements in the initial stage of the hotel guest visit experience outcomes. In line with the current information technology trends, the guests are expected to use electronic word of mouth to disseminate their visit outcomes. Academically, the study framework reduces the gaps related to the formulation of research constructs for hotel visit experience assessment. It is assertive that this paper would be practically beneficial to hotel operators or relevant stakeholders in order to gain a comprehensive understanding about hotel guests' visit experience responses and their future behaviour, including the electronic word-of-mouth response. Keywords: Commitment, electric word-of-mouth, hotel, perceived value, post visit responses, satisfaction.
MSMEs as Mediation in the Effects of Investment Credit, Interest Rates, and Labor on Economic Growth

An increase in MSMEs in large numbers is very instrumental in increasing economic growth in developing countries, including Indonesia. The research objective is based on identifying the effect of investment credit, interest rates, and labor on economic growth through the role of MSMEs. The design of this research is descriptive-verification to present a structured, factual and accurate picture and test hypotheses empirically through the MRA model. Empirical analysis proves that investment credit and interest rates through the role of MSMEs can influence positively and significantly on Indonesia's economic growth. In other results, it appears that the role of MSMEs is less able to mediate the effect of labor on Indonesia's economic growth. This fact is based on a negative and insignificant relationship. In a sentence or two, enter the implications and limitations of your research. In Indonesia, increasing bank lending to MSMEs in practice is undermined by lending policies by banks and by macroeconomic factors (economic growth, interest rates, investment credit, and labor).
SdAb heterodimer formation using leucine zippers

Single domain antibodies (sdAb) are variable domains cloned from camel, llama, or shark heavy chain only antibodies, and are among the smallest known naturally derived antigen binding fragments. SdAb derived from immunized llamas are able to bind antigens with high affinity, and most are capable of refolding after heat or chemical denaturation to bind antigen again. We hypothesized that the ability to produce heterodimeric sdAb would enable reagents with the robust characteristics of component sdAb, but with dramatically improved overall affinity through increased avidity. Previously we had constructed multimeric sdAb by genetically linking sdAb that bind non-overlapping epitopes on the toxin, ricin. In this work we explored a more flexible approach; the construction of multivalent binding reagents using multimerization domains. We expressed anti-ricin sdAb that recognize different epitopes on the toxin as fusions with differently charged leucine zippers. When the initially produced homodimers are mixed the leucine zipper domains will pair to produce heterodimers. We used fluorescence resonance energy transfer to confirm heterodimer formation. Surface plasmon resonance, circular dichroism, enzyme linked immunosorbent assays, and fluid array assays were used to characterize the multimer constructs, and evaluate their utility in toxin detection.
Notes on the marine algae of the Bermudas. 14. Five additions to the benthic flora, including a distinctive second new species of Crassitegula (Rhodophyta, Sebdeniales) from the western Atlantic Ocean

Abstract: A new red algal species, Crassitegula laciniata (Sebdeniaceae, Sebdeniales), was described from recent collections made on offshore Bermuda reef habitats, and it was distinguished morphologically and molecularly (COI-5P, LSU gene sequences) from congeners in Bermuda and Australia. In addition, four other species were reported as new members of the growing Bermuda macroalgal flora: the chlorophytes Anadyomene lacerata and Codium carolinianum and the rhodophytes Gracilaria occidentalis and Predaea goffiana.