Miracle-dz/UniFAR-FTUs huggingface dataset:
Next-generation sequencing of microRNAs uncovers expression signatures in 1 polarized macrophages 2

Next-generation sequencing of microRNAs uncovers expression signatures in 1 polarized macrophages 2 Viviana Cobos Jiménez, Edward J. Bradley, Antonius M. Willemsen, Antoine H.C. 3 van Kampen , Frank Baas and Neeltje A. Kootstra 4 Department of Experimental Immunology, Sanquin Research, Landsteiner 5 Laboratory, and Center for Infectious Diseases and Immunity Amsterdam (CINIMA), 6 Academic Medical Center of the University of Amsterdam, Amsterdam, The 7 Netherlands 8 Department of Genome Analysis, Academic Medical Center of the University of 9 Amsterdam, Amsterdam, The Netherlands 10 Bioinformatics Laboratory, Department of Clinical Epidemiology, Biostatistics and 11 Bioinformatics, Academic Medical Center of the University of Amsterdam, 12 Amsterdam, The Netherlands 13 Department of Biosystems Data Analysis, Swammerdam Institute for Life Sciences, 14 University of Amsterdam, Amsterdam, The Netherlands 15 Running Head: microRNA expression in human polarized macrophages 16 *Corresponding Author: Dr. Neeltje A. Kootstra 17 Department of Experimental Immunology (M01-120), Academic Medical Center, 18 University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. 19 Tel: +31-20-5668298; E-mail: n.a. kootstra@amc.uva. nl 20
Simultaneous determination of memantine and amantadine in human plasma as fluorescein derivatives by micellar electrokinetic chromatography with laser‐induced fluorescence detection and its clinical application

A nonionic surfactant MEKC method with LIF detection was developed for the simultaneous determination of memantine, an anti‐Alzheimer's disease agent, and amantadine, an anti‐Parkinson's disease drug, in human plasma. Before analysis, the plasma samples were pretreated by liquid–liquid extraction with ethyl acetate, and derivatized with 6‐carboxyfluorescein N‐hydroxysuccinimide ester. The chemical derivatization is performed with 6‐carboxyfluorescein N‐hydroxysuccinimide ester in ACN – 5 mM pH 9.0 borate buffer (40:60, v/v) at 35°C for 3 h. After the derivatization reaction, hydrodynamic injection (0.5 psi, 8 s) was used to introduce the derivatized solution, and the separation was performed in borate buffer (30 mM, pH 9.5) with the nonionic surfactant Brij‐35® (0.07%, w/v); the separation voltage was 6 kV. The linear ranges of the method for the determination of memantine and amantadine in human plasma were over a range of 2.0–60.0 ng/mL. The detection limit was 0.5 ng/mL (S/N=3). This method was applied successfully to monitor the concentration of memantine or amantadine in patients with Alzheimer's disease or Parkinson's disease.
Programming Projects in C - for Students of Engineering, Science, and Mathematics

Like a pianist who practices from a book of tudes, readers of Programming Projects in C for Students of Engineering, Science, and Mathematics will learn by doing. Written as a tutorial on how to think about, organize, and implement programs in scientific computing, this book achieves its goal through an eclectic and wide-ranging collection of projects. Each project presents a problem and an algorithm for solving it, and the reader is guided through implementing the algorithm in C and compiling and testing the results. It is not necessary to carry out the projects in sequential order. The projects contain suggested algorithms and partially completed programs for implementing them to enable the reader to exercise and develop skills in scientific computing; require only a working knowledge of undergraduate multivariable calculus, differential equations, and linear algebra; and are written in platform-independent standard C. The Unix command-line is used to illustrate compilation and execution. The primary audience of this book is graduate students in mathematics, engineering, and the sciences. The book will also be of interest to advanced undergraduates and working professionals who wish to exercise and hone their skills in programming mathematical algorithms in C. A working knowledge of the C programming language is assumed.
17β‐OESTRADIOL REGULATES THE EXPRESSION OF Na+/K+‐ATPase β1‐SUBUNIT, SARCOPLASMIC RETICULUM Ca2+‐ATPase AND CARBONIC ANHYDRASE IV IN H9C2 CELLS

1 It is necessary to improve our understanding of the effect of 17β‐oestradiol (E2) on the heart at a molecular and cellular level. In the present study, the effects of E2 on Na+/K+‐ATPase, sarcoplasmic/endoplasmic reticulum Ca2+‐ATPase (SERCA) and carbonic anhydrase IV (CAIV) in H9C2 cells were investigated. To identify the mechanism of action of E2 on these proteins, the oestrogen receptor (ER) antagonist tamoxifen was used. 2 The results indicated that 1 and 100 nmol/L E2 can enhance the activity of Na+/K+‐ATPase and SERCA and upregulate the expression of the Na+/K+‐ATPase β1‐subunit, SERCA2a and CAIV at both the mRNA and protein level compared with 0 and 0.01 nmol/L E2. 17β‐Oestradiol had the greatest effect at 100 nmol/L; 1 µmol/L E2 did not further protein expression compared with 100 nmol/L E2. 3 Tamoxifen (10 nmol/L) significantly decreased the activity of SERCA, as well as the expression of the Na+/K+‐ATPase β1‐subunit and SERCA at the mRNA and protein level, in H9C2 cells cultured with 1 nmol/L E2. Tamoxifen alone had no significant effect on these proteins in H9C2 cells. 4 It may be hypothesized that a suitable E2 concentration has a protective effect on the heart and that the actual dose of E2 used in hormone‐replacement therapy is important in menopausal women.
A fuzzy bi-clustering approach to correlate web users and pages

With the rapid development of information technology, the significance of clustering in the process of delivering information to users is becoming more eminent. Especially in the web information space, clustering analysis can prove particularly beneficial for a variety of applications such as web personalisation and profiling, caching and prefetching and content delivery networks. In this paper, we propose a bi-clustering approach, which identifies groups of related web users and pages. The proposed approach is a three-step process that relies on the principles of spectral clustering analysis and provides a fuzzy relation scheme for the revealed users' and pages' clusters. Experiments have been conducted on both synthetic and real datasets to prove the proposed method's efficiency and reveal hidden knowledge.
Shoulder Dystocia: An Evidence-Based Evaluation of the Obstetric Nightmare

The term “shoulder dystocia” appears to have been first used by Fieux in 1902, during a discussion of a communication of Chambrelent. As far back as 1730, however, Smellie had written: “A sudden call to a gentlewoman in labor. The child’s head delivered for a long time—but even with hard pulling from the midwife, the remarkably large shoulder prevented delivery. I have been called by midwives to many cases of this kind, in which the child was frequently lost. ” On January 15, 1879, Dr. Beech of Seville, Ohio, was called to attend to Miss Anne Swan during her second delivery. Three days later, Dr. Beech ruptured the patient’s membranes; the total amount of amniotic fluid that poured out was estimated at six gallons. An operative vaginal delivery with forceps was attempted, but they could not be applied because the vagina measured 12 inches along the posterior wall. When the shoulders were subsequently noted to be “stuck fast,” Dr. J.D. Robinson of Wooster, Ohio, arrived to complete the delivery: “It was our desire to deliver the child without mutilation so we passed a strong bandage over the neck of the child, we made strong traction downwards and laterally and finally after a laborious siege we succeeded in delivering our patient of a male child weighing 233⁄4 lbs. with a length of 30 inches. ” Shoulder dystocia continues to represent the “infrequent, unanticipated, unpredictable nightmare of the obstetrician. ” Despite its rare occurrence, each accoucheur can vividly remember at least one shoulder dystocia episode that has occurred during an obstetric career. This scary clinical phenomenon has been vividly captured by Morris: Correspondence: Robert B. Gherman, MD, Dept. of OB/GYN, Division of Maternal-Fetal Medicine, National Naval Medical Center, Bethesda Naval Hospital, 8901 Wisconsin Avenue, Bethesda, MD 20889. E-mail: ghermtoo@aol.com The views expressed in this article are those of the authors and do not reflect the official policy or position of the Department of the Navy, the Department of Defense, or the United States Government. PROD. # GRF20217
Performance Assessment of UWB-Over-Fiber and Applications

Since 2002, the Federal Communications Commission (FCC) authorized the use of ultra-wideband (UWB) signal transmissions for unlicensed use, in the range from 3.1 to 10.6 GHz, leading to a revived interest in research activities and to new opportunities for companies to explore and develop new broadband indoor and outdoor applications [1]. Moreover, UWB is seen as a promising technology for short range high speed wireless networks.
Learning Effects of Knowledge of Results upon Time Estimation

College students of both sexes served as their own controls to test three hypotheses in each of two separate experiments designed to assess the learning effects of knowledge of results upon time estimation. The results indicated that (1) knowledge of results in the form of feedback to the nearest hundredth of a second significantly increased the mean accuracy of time estimations obtained by the method of production, (2) that knowledge of results significantly decreased the variance of the time estimations, and (3) that sex difference as a main effect was not significant in either experiment. A major conclusion of the present study was that variance represents an authentic and independent measure of learning.
Spiritual Correlates of Functional Well-Being in Women With Breast Cancer

Breast cancer is the most common cancer in women in the United States and constitutes a major source of medicaland psychological morbidity. The psychosocial sequelae, while not always severe, can last up to a year after diagnosis. This may be a time when spiritual and social resources become important. It is known that practical and emotional support during treatment for breast cancer benefits the patient’s mood and quality of life, but it is unclear as to how spirituality and spiritual well-being may benefit the patient. This study examined this question by exploring the relationships between spirituality, spiritual well-being, physical well-being, functional well-being, mood, and adjustment style in a sample of 191 women recently diagnosed with breast cancer or who had metastatic cancer. The women were asked to complete questionnaires pertaining to the above topics at enrollment to a group intervention study. Measures of spirituality, spiritual well-being, physical well-being, functional well-being, mood, and adjustment style taken at the same point in time were then correlated with each other. There were more significant correlations of spirituality and spiritual well-being with functional well-being than physical well-being, but items pertaining to meaning and peace tended to correlate significantly with physical well-being. Spirituality also correlated significantly with several coping styles, but not avoidance as has been previously suggested. Regression analyses were also performed to find the best combination of variables to predict physical and functional well-being. A combination of social well-being and several questions pertaining to peacefulness accounted for 18% of the variance in physical well-being. However, a combination of social well-being and the spiritual scales accounted for 46% of the variance in functional well-being. When the spiritual scales were examined alone, they accounted for 40% of the variance in functional well-being. The results of this study confirm the importance of spirituality and spiritual well-being in both physical and functional well-being. Asking patients about the role of spirituality in their lives may be a useful marker to predict patient’s ability to cope with stress in their lives and of their quality of life.
WİNGATE ANAEROBİK GÜÇ TESTİNDE OPTİMAL YÜKÜN BELİRLENMESİ

Bu calismanin amaci, literaturdeki calismalara dayanarak Wingate Anaerobik Guc Testi (WAnT) icin en uygun optimal yukun belirlenmesidir. WAnT Israil’de, Wingate Beden Egitimi ve Spor Enstitusu’nun Arastirma ve Spor Sagligi Bolumu’nde 1970’lerde gelistirilmistir. Ilk prototipi sunuldugundan beri tum dunyada bircok laboratuarda kas gucunu, kas dayanikliligini ve yorgunlugunu belirlemekte kullanilan bir test olarak kabul gormektedir (1). WAnT uygulamasi basit, ozel becerili personel gerektirmeyen, ucuz ve kolay edinilebilir aletlerle yapilabilen, invaziv olmayan ve toplumun her kesimine, hatta cocuklara ve engellilere bile uygulanabilen bir test olarak gelistirilmistir. WAnT alt ekstremitelere oldugu kadar ust ekstremitelere de uygulanabilir. WAnT 30 saniye sure ile vucut agirligina dayanan sabit bir yuke karsi maksimum hizla pedal cevirmeyi kapsayan supramaksimal bir testtir. Uygulanacak sabit yuk, en yuksek mekanik gucu saglayacak sekilde belirlenir. Wingate Anaerobik Guc Testi’nde optimal yuku belirlerken elde edilen anaerobik guc ve anaerobik kapasite degerleri bisiklet ergometresine yerlestirilen yukten etkilenmektedir. Bu yuzden maksimal anaerobik gucun degerlendirilmesinde, her denek icin en yuksek pik guc ve ortalama guc degerlerine ulasabilecekleri yukun ayarlanmasi cok onemlidir. Wingate testi icin orjinal olarak ileri surulen yuk vucut agirliginin kg'i basina 75 gr'dir. Bu yuk antrenmansiz genclerden olusan kucuk bir grup uzerinde yapilan bir calismaya dayanarak tespit edilmistir ve cogu yetiskin icin dusuk kalmistir. Sonuc olarak literatuerde yapilan calismalarda genellikle onerilen optimal yuk sporcu olmayan erkekler icin vucut agirliginin kg'i basina 95 g.kg-1, kadinlar icin 86 g.kg-1, cocuklar icin 75 g.kg-1 iken yetiskin atletler icin 100 g.kg-1’dir
Golimumab in patients with active rheumatoid arthritis despite treatment with methotrexate: a randomized, double-blind, placebo-controlled, dose-ranging study.

OBJECTIVE To assess the efficacy, safety, and pharmacology of subcutaneous administration of golimumab in patients with active rheumatoid arthritis (RA) despite treatment with methotrexate (MTX). METHODS Patients were randomly assigned in a double-blinded manner to receive injections of placebo plus MTX or 50 mg or 100 mg golimumab every 2 or 4 weeks plus MTX through week 48. Patients originally assigned to receive injections every 2 weeks had the interval increased to every 4 weeks starting at week 20. The primary end point was the proportion of patients meeting the American College of Rheumatology 20% improvement criteria (achieving an ACR20 response) at week 16. The study was powered to detect a difference in the primary end point when the combined golimumab groups and at least 1 of the individual dose groups were compared with placebo. RESULTS The primary end point was attained. Sixty-one percent of patients in the combined golimumab plus MTX dose groups achieved an ACR20 response at week 16 compared with 37% of patients in the placebo plus MTX group (P=0.010). In addition, 79% of patients in the group receiving 100 mg golimumab every 2 weeks achieved an ACR20 response (P<0.001 versus placebo). Through week 20 (after which patients receiving placebo were switched to active infliximab therapy), serious adverse events were reported in 9% of patients in the combined golimumab groups and in 6% of patients in the placebo group. CONCLUSION Golimumab plus MTX effectively reduces the signs and symptoms of RA and is generally well tolerated in patients with an inadequate response to MTX.
Measuring Inhibitory Control in Children

The Stop Signal Task is a measure of inhibitory control in which subjects must press a button in response to a stimulus. On certain trials, the subject receives a second stimulus (the Stop Signal) after the primary stimulus and must withhold his/her response during those trials. The onset of the Stop Signal is varied, sometimes coming immediately after the primary stimulus (inhibition is easy); at other times, the Stop Signal arrives quite late, making inhibition difficult. Results from the Stop Signal Task were obtained from children with attention-deficit/hyperactivity disorder (ADHD) and from controls; children with ADHD had significantly more difficulty inhibiting their responses than did controls. In a second study, results from the Stop Signal Task were obtained from a large sample of both behaviorally disturbed and community children; variables from the Stop Signal Task correlated well with both laboratory observations and teacher ratings of inattention and hyperactivity. J Dev Behav Pediatr, 18:254–259, 1997.
Secure Data Dynamics in Tandem with Dynamic Resource Allocation

In Cloud computing both security with perfect data dynamics and optimal resource allocation are essential. For best realization of cloud computing parallel and reliable data processing is required. There are many providers of cloud services such as Oracle, Microsoft, IBM, and Google. The existing systems used for cloud computing are homogenous in nature. The resource allocation and execution of jobs parallelly has some limitations. The security is also concerns as the cloud servers are treated as un-trusted by the cloud users. In this paper parallel processing, dynamic resource allocation challenges are addressed. We built a prototype application to demonstrate the proof of concept and the empirical results are encouraging.
Energy efficiency based on joint mobile node grouping and data packet fragmentation in short‐range communication system

Energy efficiency is a key requirement for the design of short‐range network. In order to alleviate energy consumption constraint, a two‐layer short‐range communication architecture is proposed in this paper. We designed an energy‐efficient scheme on the basis of joint mobile nodes (MNs) grouping and data packet fragmentation, analyzed the energy efficiency corresponding to different packet length and group sizes, and established an appropriate energy consumption model. Simulation results show that when MNs of short‐range system are grouped by the proposed MN grouping algorithm and all nodes of each group are operating with the same size of data packet fragmentation, the short‐range communication system can provide significant energy savings. The results provide useful insights into the possible operation of the strategies and show that significant energy reductions are possible. Copyright © 2014 John Wiley & Sons, Ltd.
Enhanced Inhibition of Human Anti-Gal Antibody Binding to Mammalian Cells by Synthetic α-Gal Epitope Polymers

Neoglycopolymers of polyacrylamide backbone conjugated with varying densities of Galα1−3Galβ1−4Glcβ trisaccharide epitopes (α-Gal epitopes) were designed and synthesized to study the inhibition of the binding of human natural anti-Gal antibodies to either α-Gal-containing glycoproteins or α-Gal antigens on the surface of mammalian cells. An inhibition ELISA using mouse laminin and a flow cytometry assay using pig kidney cells (PK15) were established to determine the binding affinity of the synthesized polymers. In comparison to the α-Gal monomer (Galα1−3Galβ1−4GlcNHAcβ), the α-Gal polymers dramatically enhanced the inhibition of human anti-Gal antibodies (IgG, IgM, and IgA) binding to mouse laminin or mammalian cells. Increases of 7.8 × 103- and 5.0 × 104 -fold in inhibitory potential of polymer 7C to IgA and IgM (with IC50s of 7.0 and 5.6 nM respectively) were observed over the monomer in inhibition ELISA. The results also indicated that binding enhancement of α-Gal polymers is greater for anti-Gal IgA a.
Measurement of Sap Flow in Conifers by Heat Transport.

An account of the various methods which have been used in an attempt to measure the rate of sap flow in plants has been given by Crafts, Currier and Stocking (2). Methods which require the measurement of the amount of material moving through a known cross-sectional area, or the timing of the movement of some material indicator such as salts, dyes or radioactive material, suffer from the disadvantage that the vessels conveying the sap stream must be severed, either to measure the quantity of sap or to introduce the indicator, causing an unknown disturbance to the initial state. Our experience confirms that on cutting the vessels of a living tree the rate of sap flow always changes greatly in magnitude, often instantaneously. Heat used as an indicator, however, does not have this disadvantage as it can enter the sap stream by thermal conduction through the walls of the vessels. Measurements using heat have been made by Huber (5) and his school (e.g., Huber and Schmidt (6)), and Dixon (3). Huberts first experiments were made on a tropical liana with such a high rate of sap flow that heat applied for one or two seconds was still recognizable as a pulse at the junctions of a thermocouple 30 cm downstream from the heater. The time till the first appearance of heat at the thermocouple was assumed to be the same as the time taken for the sap to move this distance. For slower sap speeds it became essential to distinguish between the effect of convection by the moving sap and the transport of heat by thermal conduction. Dixon achieved this by placing the thermoj unctions at distances of 1 cm and 2 cm from the heater and considering only cases when the more distant, junction at some stage indicated a higher temperature than the nearer one. Huber and Schmidt (6) devised a method for the same purpose in which one junction was 2.0 cm downstream and the other 1.6 cm upstream from the heater. In all these experiments the heater wires and the thermojunctions were laid either on the intact bark or just under the bark on the surface of the sap wood. There are two fundamental drawbacks to these heat transport experiments. First, it will be shown below that the assumption made in each case that the speed of the sap is identical with that of the heat pulse is not justified. In conifers the sap speed is actually about three times that of the heat pulse. Secondly, comparison between the curves presented for galvanometer deflections versus time and those calculated theoretically shows that, in spite of cotton wool lagging, there was considerable loss of heat; this reduces the usefulness of the results.
Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution.

Mutational studies indicate that the superantigen staphylococcal enterotoxin A (SEA) has two separate binding sites for major histocompatibility complex (MHC) class II molecules. Direct evidence is provided here for the formation of SEA-MHC class II trimers in solution. Isoelectric focusing separated SEA-HLA-DR1 complexes into both dimers and HLA-DR1.SEA2 trimers. The molar ratio of components was determined by dual isotope labeling. The SEA mutant SEA-F47S, L48S, Y92A, which is deficient in MHC class II alpha-chain binding, formed only dimers with HLA-DR1, whereas a second SEA mutant, SEA-H225A, which lacks high-affinity MHC class II beta-chain binding was incapable of forming any complexes. Thus SEA binding to its MHC receptor is a two-step process involving initial beta-chain binding followed by cooperative binding of a second SEA molecule to the class II alpha chain.