Miracle-dz/UniFAR-FTUs huggingface dataset:
Korean Traditional Medicine (Jakyakgamcho-tang) Ameliorates Colitis by Regulating Gut Microbiota

The objective of this study was to examine the anti-colitis activity of Jakyakgamcho-tang (JGT) in dextran sulfate sodium (DSS)-induced colitis and explore changes of the gut microbial community using 16S rRNA amplicon sequencing and metabolomics approaches. It was found that treatment with JGT or 5-aminosalicylic acid (5-ASA) alleviated the severity of colitis symptoms by suppressing inflammatory cytokine levels of IL-6, IL-12, and IFN-γ. The non-metric multidimensional scaling analysis of gut microbiome revealed that JGT groups were clearly separated from the DSS group, suggesting that JGT administration altered gut microbiota. The operational taxonomic units (OTUs) that were decreased by DSS but increased by JGT include Akkermansia and Allobaculum. On the other hand, OTUs that were increased by DSS but decreased by 5-ASA or JGT treatments include Bacteroidales S24-7, Ruminococcaceae, and Rikenellaceae, and the genera Bacteroides, Parabacteroides, Oscillospira, and Coprobacillus. After JGT administration, the metabolites, including most amino acids and lactic acid that were altered by colitis induction, became similar to those of the control group. This study demonstrates that JGT might have potential to effectively treat colitis by restoring dysbiosis of gut microbiota and host metabolites.
Biased cognition in East Asian and Western cultures

The majority of cognitive bias research has been conducted in Western cultures. We examined cross-cultural differences in cognitive biases, comparing Westerners’ and East Asians’ performance and acculturation following migration to the opposite culture. Two local (UK, Hong Kong) and four migrant (short-term and long-term migrants to each culture) samples completed culturally validated tasks measuring attentional and interpretation bias. Hong Kong residents were more positively biased than people living in the UK on several measures, consistent with the lower prevalence of psychological disorders in East Asia. Migrants to the UK had reduced positive biases on some tasks, while migrants to Hong Kong were more positive, compared to their respective home counterparts, consistent with acculturation in attention and interpretation biases. These data illustrate the importance of cultural validation of findings and, if replicated, would have implications for the mental health and well-being of migrants.
A novel MYCN-specific antigene oligonucleotide deregulates mitochondria and inhibits tumor growth in MYCN-amplified Neuroblastoma.

Approximately half of high-risk neuroblastoma (NB) is characterized by MYCN-amplification. N-Myc promotes tumor progression by inducing cell growth and inhibiting differentiation. MYCN has also been shown to play an active role in mitochondrial metabolism, but this relationship is not well understood. While N-myc is a known driver of the disease, it remains a target for which no therapeutic drug exists. Here, we evaluated a novel MYCN-specific antigene PNA oligonucleotide (BGA002) in MYCN-amplified (MNA) or MYCN-expressing NB, and investigated the mechanism of its anti-tumor activity. MYCN mRNA and cell viability were reduced in a broad set of NB cell lines following BGA002 treatment. Furthermore, BGA002 decreased N-myc protein levels and apoptosis in MNA-NB. Analysis of gene expression data from neuroblastoma patients revealed that MYCN was associated with increased reactive oxygen species (ROS), downregulated mitophagy and poor prognosis. Inhibition of MYCN caused profound mitochondrial damage in MNA-NB cells through downregulation of the mitochondrial molecular chaperone TRAP1, which subsequently increased ROS. Correspondigly, inhibition of MYCN reactivated mitophagy. Systemic administration of BGA002 downregulated N-myc and TRAP1 with a concomitant decrease in MNA-NB xenograft tumor weight. In conclusion, this study highlights the role of N-myc in blocking mitophagy in NB and in conferring protection to ROS in mitochondria through upregulation of TRAP1. BGA002 is a potently improved MYCN-specific antigene oligonucleotide that reverts N-myc dysregulated mitochondrial pathways, leading to loss of the protective effect of N-myc against mitochondrial ROS.
Ultrafast Large-Scale Chemical Sensing With CMOS ISFETs: A Level-Crossing Time-Domain Approach

The introduction of large-scale chemical sensing systems in CMOS which integrate millions of ISFET sensors have allowed applications such as DNA sequencing and fine-pixel chemical imaging systems to be realised. Using CMOS ISFETs provides advantages of digitisation directly at the sensor as well as correcting for non-linearity in its response. However, for this to be beneficial and scale, the readout circuits need to have the minimum possible footprint and power consumption. Within this context, this paper analyses an ISFET based pH-to-time readout using an inverter in the time-domain as a level-crossing detector and presents a 32 × 32 array with in-pixel digitisation for pH sensing. The inverter-based sensing pixel, controlled by a triangular waveform, converts the pH response into a time-domain signal whilst also compensating for sensor offset and thus resulting in an increase in dynamic range. The sensor pixels interface to a 15-bit asynchronous column-wise time-to-digital converter (TDC), enabling fast asynchronous conversion whilst using minimal silicon area. Parallel outputs of 32 TDC interfaces are serialised to achieve fast data throughput. This system is implemented in a standard 0.18<inline-formula><tex-math notation="LaTeX">$\,\mu$</tex-math></inline-formula>m CMOS technology, with a pixel size of 26 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m × 26 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m and a TDC area of 26 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m × 180 <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>m. Additionally, we investigate the use of additional offset compensation by having half of the array implemented with the floating gate tied down via a well diode. Measured results demonstrate the system is able to sense reliably with an average pH sensitivity of 30 mV/pH, whilst being able to compensate for sensor offset by up to <inline-formula><tex-math notation="LaTeX">$\pm$</tex-math></inline-formula>7 V. A resolution of 0.013 pH is achieved and noise measurements show an integrated noise of 0.08 pH within 2–500 Hz and SFDR of 42.6 dB. The total power consumption of the system is measured to be 11.286 mW when operating at a high frame rate of 1 KFPS.
Transcriptional Modulation of the Host Immunity Mediated by Cytokines and Transcriptional Factors in Plasmodium falciparum-Infected Patients of North-East India

Complications due to malaria are caused mostly by host immunological responses. Plasmodium falciparum subverts host immunity by various strategies, including modulation in the host immune responses by regulating cytokines. The transcriptional alterations of major cytokines and immunoregulators were analyzed in this study through gene expression profiling in clinically defined subgroups of P. falciparum patients. Malaria patients were included from Dhalai district hospital of Tripura with uncomplicated malaria (UC) and severe malaria (SM) and healthy controls from endemic and non-endemic areas of India. qPCR gene expression analysis was performed for all factors and they were grouped into three clusters based on their altered expressions. The first cluster was downregulated with an increased parasitic burden which included T-BET, GATA3, EOMES, TGF-β, STAT4, STAT6 and cytokines IFN-γ, IL-12, IL-4, IL-5, and IL-13. RANTES, IL-8, CCR8, and CXCR3 were decreased in the SM group. The second cluster was upregulated with severity and included TNF-α, IL-10, IL-1β and IL-7. PD-1 and BCL6 were increased in the SM group. The third cluster comprised of NF-κB and was not altered. The level of perforin was suppressed while GrB expression was elevated in SM. P. falciparum malaria burden is characterized by the modulation of host immunity via compromization of T cell-mediated responses and suppression of innate immune-regulators.
Dual drug-loaded biodegradable Janus particles for simultaneous co-delivery of hydrophobic and hydrophilic compounds

Bicompartmental Janus particles have many advantages in drug delivery, including co-delivery of two compounds with varying solubilities, differential release kinetics, and two surfaces available for targeting ligands. We present a novel strategy using the double emulsion method for the coencapsulation and staggered release of a hydrophobic and hydrophilic drug from anisotropic PLGA/PCL Janus particles, as well as a UV detection method to measure the release of two different compounds from Janus particles. Curcumin and quercetin were chosen as the model hydrophobic compounds for drug loading studies, while acetaminophen (APAP) and naproxen were chosen as the model hydrophilic–hydrophobic drug pair for encapsulation methods and drug loading. Also, a similar double emulsion method was also applied for PLGA/Preicrol® Janus particles containing Doxorubicin and Curcumin. Hydrophobic drugs were encapsulated by the single O/W emulsion technique. Hydrophilic compounds required special modifications due to their poor oil solubility and tendency to escape to the outer aqueous phase during the emulsification and solvent evaporation steps. In total, three different strategies for incorporating hydrophilic drugs were employed: (1) O/W emulsion with partially water miscible solvent, (2) O/W emulsion with co-solvent (i.e. acetone, methanol, ethanol), or (3) W/O/W double emulsion. The encapsulation efficiencies and drug loading percentages were measured using UV/Vis spectroscopy and compared for the different synthesis methods. It was found that the double emulsion method resulted in the highest encapsulation efficiency and drug loading of the hydrophilic drug.
Intensification of dairy production can increase the GHG mitigation potential of the land use sector in East Africa

Sub‐Saharan Africa (SSA) could face food shortages in the future because of its growing population. Agricultural expansion causes forest degradation in SSA through livestock grazing, reducing forest carbon (C) sinks and increasing greenhouse gas (GHG) emissions. Therefore, intensification should produce more food while reducing pressure on forests. This study assessed the potential for the dairy sector in Kenya to contribute to low‐emissions development by exploring three feeding scenarios. The analyses used empirical spatially explicit data, and a simulation model to quantify milk production, agricultural emissions and forest C loss due to grazing. The scenarios explored improvements in forage quality (Fo), feed conservation (Fe) and concentrate supplementation (Co): FoCo fed high‐quality Napier grass (Pennisetum purpureum), FeCo supplemented maize silage and FoFeCo a combination of Napier, silage and concentrates. Land shortages and forest C loss due to grazing were quantified with land requirements and feed availability around forests. All scenarios increased milk yields by 44%–51%, FoCo reduced GHG emission intensity from 2.4 ± 0.1 to 1.6 ± 0.1 kg CO2eq per kg milk, FeCo reduced it to 2.2 ± 0.1, whereas FoFeCo increased it to 2.7 ± 0.2 kg CO2eq per kg milk because of land use change emissions. Closing the yield gap of maize by increasing N fertilizer use reduced emission intensities by 17% due to reduced emissions from conversion of grazing land. FoCo was the only scenario that mitigated agricultural and forest emissions by reducing emission intensity by 33% and overall emissions by 2.5% showing that intensification of dairy in a low‐income country can increase milk yields without increasing emissions. There are, however, risks of C leakage if agricultural and forest policies are not aligned leading to loss of forest to produce concentrates. This approach will aid the assessment of the climate‐smartness of livestock production practices at the national level in East Africa.
Household Smoking Status and Heavy Metal Concentrations in Toenails of Children

There is limited evidence on the distribution of heavy metals and its association with secondhand smoking (SHS) within Pacific Island children living in New Zealand. Certain heavy metals such as cadmium (Cd), lead (Pb), and aluminum (Al) bioaccumulates in the body and can deteriorate health in both children and adults. Others, such as chromium (Cr) and nickel (Ni) in trace amounts are necessary but become toxic at high levels. Exposure routes of these elements include food, water, and air. The purpose of this study was to identify the distribution of toxic metal concentrations and its possible correlation with SHS within the Pacific Island children. A sub-sample of children within Pacific Island families longitudinal study, at the nine-year phase, who were living in the New Zealand city of Auckland were invited to participate, (n = 278). Toenails were used as a biomarker to determine Cr, Pb, Cd, Cu, Ni, and Al concentration using inductively coupled plasma mass spectrometry. Reliable and validated questionnaires were used for demographics, lifestyle, and health outcome variables. Significant differences between household smoking status and ethnicity, as well as parents’ marital status, were observed (p < 0.05). There was no statistical difference in heavy metal concentrations in smoking versus non-smoking households. However, Cr, Pb, Cd, Cu, and Ni concentrations were all higher than the required optimal health value in both groups. A high concentration of heavy metals was observed in these children that exceeded the value required for optimal health, although no significant difference in heavy metals with regards to secondhand smoking was observed. SHS was associated with children’s ethnicity and parental marital status, but not with household income levels or maternal education.
Temperature Impact in LoRaWAN—A Case Study in Northern Sweden

LoRaWAN has become popular as an IoT enabler. The low cost, ease of installation and the capacity of fine-tuning the parameters make this network a suitable candidate for the deployment of smart cities. In northern Sweden, in the smart region of Skellefteå, we have deployed a LoRaWAN to enable IoT applications to assist the lives of citizens. As Skellefteå has a subarctic climate, we investigate how the extreme changes in the weather happening during a year affect a real LoRaWAN deployment in terms of SNR, RSSI and the use of SF when ADR is enabled. Additionally, we evaluate two propagation models (Okumura-Hata and ITM) and verify if any of those models fit the measurements obtained from our real-life network. Our results regarding the weather impact show that cold weather improves the SNR while warm weather makes the sensors select lower SFs, to minimize the time-on-air. Regarding the tested propagation models, Okumura-Hata has the best fit to our data, while ITM tends to overestimate the RSSI values.
Experimental Evaluation on the Heating Efficiency of Magnetoferritin Nanoparticles in an Alternating Magnetic Field

The superparamagnetic substance magnetoferritin is a potential bio-nanomaterial for tumor magnetic hyperthermia because of its active tumor-targeting outer protein shell, uniform and tunable nanosized inner mineral core, monodispersity and good biocompatibility. Here, we evaluated the heating efficiency of magnetoferritin nanoparticles in an alternating magnetic field (AMF). The effects of core-size, Fe concentration, viscosity, and field frequency and amplitude were investigated. Under 805.5 kHz and 19.5 kA/m, temperature rise (ΔT) and specific loss power (SLP) measured on magnetoferritin nanoparticles with core size of 4.8 nm at 5 mg/mL were 14.2 °C (at 6 min) and 68.6 W/g, respectively. The SLP increased with core-size, Fe concentration, AMF frequency, and amplitude. Given that: (1) the SLP was insensitive to viscosity of glycerol-water solutions and (2) both the calculated effective relaxation time and the fitted relaxation time were closer to Néel relaxation time, we propose that the heating generation mechanism of magnetoferritin nanoparticles is dominated by the Néel relaxation. This work provides new insights into the heating efficiency of magnetoferritin and potential future applications for tumor magnetic hyperthermia treatment and heat-triggered drug release.
Water Source Preferences and Water Quality Perceptions among Women in the Eastern Region, Ghana: A Grounded Theory Study

Residents in the Eastern Region, Ghana with access to improved water sources (e.g., boreholes and covered wells) often choose to collect water from unimproved sources (e.g., rivers and uncovered wells). To assess why, we conducted two field studies to coincide with Ghana’s rainy and dry seasons. During the rainy season, we conducted semi-structured in-depth interviews among a convenience sample of 26 women in four rural communities (including one woman in the dry season). We asked each participant about their attitudes and perceptions of water sources. During the dry season, we observed four women for ≤4 days each to provide context for water collection and water source choice. We used a grounded theory approach considering the multiple household water sources and uses approach to identify three themes informing water source choice: collection of and access to water, water quality perception, and the dynamic interaction of these. Women selected water sources based on multiple factors, including season, accessibility, religious/spiritual messaging, community messaging (e.g., health risks), and ease-of-use (e.g., physical burden). Gender and power dynamics created structural barriers that affected the use of unimproved water sources. A larger role for women in water management and supply decision-making could advance population health goals.
Can Ciprofloxacin be Used for Precision Treatment of Gonorrhea in Public STD Clinics? Assessment of Ciprofloxacin Susceptibility and an Opportunity for Point-of-Care Testing

Background: Given the lack of new antimicrobials to treat Neisseria gonorrhoeae (NG) infections, reusing previously recommended antimicrobials has been proposed as a strategy to control the spread of multi-drug-resistant NG. We assessed ciprofloxacin susceptibility in a large sample set of NG isolates and identified correlates associated with ciprofloxacin-resistant NG infections. Methods: NG isolates collected in Baltimore, Maryland between 2014 and 2016 were evaluated by Gyrase A (gyrA) PCR and E-test for susceptibility to ciprofloxacin. Clinical characteristics and demographics were evaluated by multivariate regression analysis to identify correlates of ciprofloxacin-resistant NG infections. Results: 510 NG isolates from predominately African American (96.5%), heterosexual (85.7%), and HIV-negative (92.5%) male subjects were included in the study. The overall percentage of isolates with mutant gyrA sequences, indicative of ciprofloxacin resistance, was 32.4%, and significantly increased from 24.7% in 2014 to 45.2% in 2016 (p < 0.001). Participants older than 35 years of age were 2.35 times more likely to have a gyrA mutant NG infection than younger participants (p < 0.001). Race, sexual orientation, symptomology, or co-infection the HIV or syphilis were not associated with a particular NG gyrA genotype. Conclusions: Resistance to ciprofloxacin in Baltimore is lower than other regions and indicates that in this environment, use of ciprofloxacin may be appropriate for targeted treatment provided utilization of enhanced surveillance tools. The targeted use of ciprofloxacin may be more beneficial for individuals under 35 years of age. Point-of-care tests for NG diagnosis and susceptibility testing are urgently needed to identify individuals who can be treated with this targeted approach.
Smart Hydrogels in Tissue Engineering and Regenerative Medicine

The field of regenerative medicine has tremendous potential for improved treatment outcomes and has been stimulated by advances made in bioengineering over the last few decades. The strategies of engineering tissues and assembling functional constructs that are capable of restoring, retaining, and revitalizing lost tissues and organs have impacted the whole spectrum of medicine and health care. Techniques to combine biomimetic materials, cells, and bioactive molecules play a decisive role in promoting the regeneration of damaged tissues or as therapeutic systems. Hydrogels have been used as one of the most common tissue engineering scaffolds over the past two decades due to their ability to maintain a distinct 3D structure, to provide mechanical support for the cells in the engineered tissues, and to simulate the native extracellular matrix. The high water content of hydrogels can provide an ideal environment for cell survival, and structure which mimics the native tissues. Hydrogel systems have been serving as a supportive matrix for cell immobilization and growth factor delivery. This review outlines a brief description of the properties, structure, synthesis and fabrication methods, applications, and future perspectives of smart hydrogels in tissue engineering.
Proteoglycan/glycosaminoglycan and collagen content in the arterial wall of patients with end-stage renal disease - new indicators for vascular disease.

INTRODUCTION Cardiovascular (CV) comorbidity is high in chronic kidney disease, particularly in end-stage renal disease (ESRD). Novel biomarkers are of interest. OBJECTIVES Investigate relationships between matrix proteoglycans/glycosaminoglycans (PG/GAGs), collagen and calcifications in arteries with selected serum and plasma markers of endothelial dysfunction, inflammation, oxidative stress and bone turnover in patients with ESRD. PATIENTS AND METHODS 47 adult patients (32 male) with stage 5 chronic kidney disease were included. Fibrinogen, soluble thrombomodulin (sTM), plasminogen activator inhibitor-1 (PAI-1), stromal cell-derived factor-1 alpha (SDF-1α), calcium (Ca), phosphate (Pi), intact parathormone (iPTH), interleukin-6 (IL-6), highly sensitive C-reactive protein (hsCRP), ferric reducing ability of plasma (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, ferric reducing ability of ascorbate in plasma (FRASC), fetuin-A, fibroblast growth factor 23 (FGF-23), osteopontin (OPN), osteoprotegerin (OPG), osteocalcin (OC), transforming growth factor-β (TGF-β), hepatocyte growth factor (HGF), secreted protein acidic and rich in cysteine (SPARC), and matrix metalloproteinase 2 (MMP-2) were investigated. Radial artery specimens were stained with alizarin red for calcifications, alcian blue for PGs/GAGs and sirius red for collagen. RESULTS PGs/GAGs, collagen, and calcification stainings were all positively inter-correlated. Most intense (grade 3) alcian blue staining was significantly associated with diabetes, higher CaxPi, hsCRP, fibrinogen, SDF-1α, PAI-1, and thrombomodulin. PAI-1 was the only significant predictor of grade 3 alcian blue in multiple logistic regression adjusted for hemodialysis, CaxPi and hsCRP. CONCLUSIONS Coagulative and endothelial dysfunction are hallmarks of ESRD. SDF-1α, PAI-1, thrombomodulin and fibrinogen may be novel risk indicators for early vascular wall alterations and serve as CV risk biomarkers.