Miracle-dz/UniFAR-FTUs huggingface dataset:
Sex-dependent effects of genetic upregulation of activated protein C on delayed effects of acute radiation exposure in the mouse heart, small intestine, and skin

Accidental exposure to ionizing radiation may lead to delayed effects of acute radiation exposure (DEARE) in many organ systems. Activated protein C (APC) is a known mitigator of the acute radiation syndrome. To examine the role of APC in DEARE, we used a transgenic mouse model with 2- to 3-fold increased plasma levels of APC (high in APC, APCHi). Male and female APCHi mice and wild-type littermates were exposed to 9.5 Gy γ-rays with their hind-legs (bone marrow) shielded from radiation to allow long-term survival. At 3 and 6 months after irradiation, cardiac function was measured with ultrasonography. At 3 months, radiation increased cardiac dimensions in APCHi males, while decreases were seen in wild-type females. At this early time point, APCHi mice of both sexes were more susceptible to radiation-induced changes in systolic function compared to wild-types. At 6 months, a decrease in systolic function was mainly seen in male mice of both genotypes. At 6 months, specimens of heart, small intestine and dorsal skin were collected for tissue analysis. Female APCHi mice showed the most severe radiation-induced deposition of cardiac collagens but were protected against a radiation-induced loss of microvascular density. Both male and female APCHi mice were protected against a radiation induced upregulation of toll-like receptor 4 in the heart, but this did not translate into a clear protection against immune cell infiltration. In the small intestine, the APCHi genotype had no effect on an increase in the number of myeloperoxidase positive cells (seen mostly in females) or an increase in the expression of T-cell marker CD2 (males). Lastly, both male and female APCHi mice were protected against radiation-induced epidermal thickening and increase in 3-nitrotyrosine positive keratinocytes. In conclusion, prolonged high levels of APC in a transgenic mouse model had little effects on indicators of DEARE in the heart, small intestine and skin, with some differential effects in male compared to female mice.
Effect of the FIFA 11+ soccer specific warm up programme on the incidence of injuries: A cluster-randomised controlled trial

Background Soccer players incur injuries that typically affect their performance. Injuries are caused by intrinsic and extrinsic factors that call for multifactorial preventive interventions. The study examines the impact of the FIFA 11+ warm up programme on the incidence and severity of injuries in second division soccer players in Rwanda. Methods Twelve teams (309 players) were randomised in the intervention group and 12 teams (317 players) in the control group using a cluster randomized controlled trial with teams as the unit of randomization. Intervention group teams implemented the FIFA 11+ soccer specific warm-up programme during training and matches at least three times a week over seven months of the Rwandan soccer season. Control group teams continued with usual warm up exercises. The primary outcome of this study was the overall incidence of training and match injuries. Injuries, training and match exposure as well as severity categories were recorded per the F-MARC guidelines. Results A lower proportion of players sustained injuries in the intervention group (52%) compared to the control group (63%) (Odd ratio: 0.7; 95%CI: 0.5–0.9). A significantly lower rate ratio was observed in the intervention group for overall (RR = 0.6; 95%CI: 0.5–0.8) and match (RR = 0.6; 95%CI: 0.5–0.8) injuries. Compliance to the injury prevention programme was 77%. In the intervention group, the incidence of injury was similar across all teams and across the medium and highly compliant teams. There was a statistically significant 55% and 71% reduction of the rate of moderate and severe injuries in the intervention group respectively. Conclusion The 11+ warm up injury prevention programme resulted in a significant reduction in the odds of sustaining injuries. In addition, injuries sustained were less severe. The programme should be rolled out to all teams in Rwanda and may well result in a decrease in the incidence and severity of injury in similar contexts. Trial registration Pan African Clinical Trial Registry (PACTR201505001045388).
Genotoxic effect induced by dried nicotiana tabacum leaves from tobacco barns (kiln-houses) in chinese hamster lung fibroblast cells (V79)

ABSTRACT Nicotiana tabacum is the most cultivated tobacco species in the state of Rio Grande do Sul, Brazil. Workers who handle the plant are exposed to the leaf components during the harvesting process and when separating and classifying the dried leaves. In addition to nicotine, after the drying process, other components may be found including tobacco-specific nitrosamines, polycyclic aromatic hydrocarbons, as well as pesticides residues. The objective of this study was to examine the genotoxicity attributed to the aqueous extract of dried tobacco leaves obtained from tobacco barns using Chinese hamster lung fibroblast cells (V79) as a model system by employing alkaline comet assay, micronucleus (MN) and Ames test. MTT assay was used to assess cytotoxicity and establish concentrations for this study. Data demonstrated cell viability > 85% for concentrations of 0.625–5 mg/ml while the comet assay indicated a significant increase in DNA damage at all concentrations tested. A significant elevation of MN and nuclear buds (NBUD) was found for 5 mg/ml compared to control and other dry tobacco leaves concentrations (0.625–2.5 mg/ml). Mutagenicity was not found using the Salmonella/Microsome test (TA98, TA100, and TA102 strains) with and without metabolic activation. The concentration of inorganic elements was determined employing the PIXE technique, and 13 inorganic elements were detected. Using CG/MS nicotine amounts present were 1.56 mg/g dry tobacco leaf powder. Due to the observed genotoxicity in V79 cells, more investigations are needed to protect the health of tobacco workers exposed daily to this complex mixture of toxic substances present in dry tobacco leaves.
QSAR models for predicting the toxicity of halogenated phenols to Tetrahymena

ABSTRACT Halogenated phenols are industrial pollutants which may cause serious damage to the environment. In this work, a two-dimensional (2D) and two three-dimensional (3D) quantitative structure–activity relationship models for the toxicity prediction of halogenated phenols in Tetrahymena were developed. The structural factors that mainly influence toxicity were explored, agreeing with previously reported data. The results obtained with the 3D model were in agreement with those obtained with the 2D model. According to our findings, several new molecules with different predicted activities were designed.
Diet plays a central role in parental self‐treatment of children with Hirschsprung’s disease—a qualitative study

Hirschsprung's disease is a congenital disorder requiring surgery. Most children operated on for Hirschsprung's disease experience postoperative bowel dysmotility. Although various food is known to influence bowel motility, evidence of diet's role and dietary guidelines in treatment of bowel dysfunction in Hirschsprung's disease is lacking. The aim was to explore parental experiences of dietary effects on bowel function in children with Hirschsprung's disease.
Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression, and cell proliferation.

Cell cycle control is a key aspect of numerous physiological and pathological processes. The contribution of biophysical cues, such as stiffness or elasticity of the underlying extracellular matrix (ECM), is critically important in regulating cell cycle progression and proliferation. Indeed, increased ECM stiffness causes aberrant cell cycle progression and proliferation. However, the molecular mechanisms that control these stiffness-mediated cellular responses remain unclear. Here, we address this gap and show good evidence that lamellipodin, previously known as a critical regulator of cell migration, stimulates ECM stiffness-mediated cyclin expression and intracellular stiffening. We observed that increased ECM stiffness upregulates lamellipodin expression. This is mediated by an integrin-dependent FAK-Cas-Rac signaling module and supports stiffness-mediated lamellipodin induction. Mechanistically, we find that lamellipodin overexpression increased and lamellipodin knockdown reduced stiffness-induced cell cyclin expression and cell proliferation, and intracellular stiffness. Overall, these results suggest that lamellipodin levels may be critical for regulating cell proliferation.
Evidence for 5‐HT1A receptor‐mediated antiallodynic and antihyperalgesic effects of apigenin in mice suffering from mononeuropathy

Neuropathic pain places a devastating health burden, with very few effective therapies. We investigated the potential antiallodynic and antihyperalgesic effects of apigenin, a natural flavonoid with momoamine oxidase (MAO) inhibitory activity, against neuropathic pain and investigated the mechanism(s).
Program sustainability post PEPFAR direct service support in the Western Cape, South Africa

Background Public health practitioners have little guidance around how to plan for the sustainability of donor sponsored programs after the donor withdraws. The literature is broad and provides no consensus on a definition of sustainability. This study used a mixed-methods methodology to assess program sustainability factors to inform donor-funded programs. Methods This study examined 61 health facilities in the Western Cape, South Africa, supported by four PEPFAR-funded non-governmental organizations from 2007 to 2012. Retention in care (RIC) was used to determine health facility performance. Sustainability was measured by comparing RIC during PEPFAR direct service (20072012), to RIC in the post PEPFAR period (2013 to 2015). Forty-three semi-structured in-depth interviews were conducted with key informants. The qualitative data were used to examine how predictor variables were operationalized at a health facility and NGO level. Results Our qualitative results suggest the following lessons for the sustainability of future programs: Sufficient and stable resources (i.e., financial, human resources, technical expertise, equipment, physical space) Investment in organizations that understand the local context and have strong relationships with local government. Strong leadership at a health facility level Joint planning/coordination and formalized skill transfer Local positive perceived value of the program Partnerships Conclusion Sustainability is complex, context dependent, and is reliant on various processes and outcomes. This study suggests additional health facility and community level staff should be employed in the health system to ensure RIC sustainability. Sustainability requires joint donor coordination with experienced local organizations with strong managers before during and after program implementation. If the program is as large as the South African HIV effort some dedicated additional resources in the long term would be required.
Time-dependent viral interference between influenza virus and coronavirus in the infection of differentiated porcine airway epithelial cells

ABSTRACT Coronaviruses and influenza viruses are circulating in humans and animals all over the world. Co-infection with these two viruses may aggravate clinical signs. However, the molecular mechanisms of co-infections by these two viruses are incompletely understood. In this study, we applied air-liquid interface (ALI) cultures of well-differentiated porcine tracheal epithelial cells (PTECs) to analyze the co-infection by a swine influenza virus (SIV, H3N2 subtype) and porcine respiratory coronavirus (PRCoV) at different time intervals. Our results revealed that in short-term intervals, prior infection by influenza virus caused complete inhibition of coronavirus infection, while in long-term intervals, some coronavirus replication was detectable. The influenza virus infection resulted in (i) an upregulation of porcine aminopeptidase N, the cellular receptor for PRCoV and (ii) in the induction of an innate immune response which was responsible for the inhibition of PRCoV replication. By contrast, prior infection by coronavirus only caused a slight inhibition of influenza virus replication. Taken together, the timing and the order of virus infection are important determinants in co-infections. This study is the first to show the impact of SIV and PRCoV co- and super-infection on the cellular level. Our results have implications also for human viruses, including potential co-infections by SARS-CoV-2 and seasonal influenza viruses.
Predicting Brain Age Using Machine Learning Algorithms: A Comprehensive Evaluation

Machine learning (ML) algorithms play a vital role in the brain age estimation frameworks. The impact of regression algorithms on prediction accuracy in the brain age estimation frameworks have not been comprehensively evaluated. Here, we sought to assess the efficiency of different regression algorithms on brain age estimation. To this end, we built a brain age estimation framework based on a large set of cognitively healthy (CH) individuals (<inline-formula><tex-math notation="LaTeX">$N = 788$</tex-math></inline-formula>) as a training set followed by different regression algorithms (22 different algorithms in total). We then quantified each regression-algorithm on independent test sets composed of 88 CH individuals, 70 mild cognitive impairment patients as well as 30 Alzheimer’s disease patients. The prediction accuracy in the independent test set (i.e., CH set) varied in regression algorithms mean absolute error (MAE) from 4.63 to 7.14 yrs, <inline-formula><tex-math notation="LaTeX">$R^2$</tex-math></inline-formula> from 0.76 to 0.88. The highest and lowest prediction accuracies were achieved by Quadratic Support Vector Regression algorithm (MAE <inline-formula><tex-math notation="LaTeX">$= 4.63$</tex-math></inline-formula> yrs, <inline-formula><tex-math notation="LaTeX">$R^2 = 0.88, 95\%$</tex-math></inline-formula> CI <inline-formula><tex-math notation="LaTeX">$= [-1.26, 1.42]$</tex-math></inline-formula>) and Binary Decision Tree algorithm (MAE <inline-formula><tex-math notation="LaTeX">$= 7.14$</tex-math></inline-formula> yrs, <inline-formula><tex-math notation="LaTeX">$R^2 = 0.76, 95\%$</tex-math></inline-formula> CI <inline-formula><tex-math notation="LaTeX">$= [-1.50, 2.62]$</tex-math></inline-formula>), respectively. Our experimental results demonstrate that the prediction accuracy in brain age frameworks is affected by regression algorithms, indicating that advanced machine learning algorithms can lead to more accurate brain age predictions in clinical settings.
Pathogenesis and characteristics of large ameloblastoma of the jaw: a report of two rare cases

Ameloblastoma is a common odontogenic epithelial tumor that exhibits various biological behaviors, ranging from simple cystic expansion to aggressive solid masses characterized by local invasiveness, a high risk of recurrence, and even malignant transformation. We report on two cases of unusually large solid ameloblastomas. We detected epithelial–mesenchymal transition-related gene expression and HRAS gene single nucleotide polymorphisms, providing possible molecular evidence of mesenchymal morphological changes in ameloblastoma. The detailed analysis of the pathogenesis of these two cases of ameloblastoma may deepen our understanding of this rare disease and offer promising targets for future targeted therapy.
Combining ECG and echocardiography to identify transthyretin cardiac amyloidosis in heart failure

Transthyretin amyloid (ATTR) amyloidosis cardiomyopathy is an underdiagnosed, causatively treatable cause of heart failure (HF). The aim of this study was to evaluate the efficacy of electrocardiogram (ECG) and echocardiography on patients with increased interventricular septum diameter (IVSd) to identify ATTR cardiac amyloidosis (ATTR‐CA) patients.
Endocytic adaptation to functional demand by the kidney proximal tubule

The kidney proximal tubule (PT) efficiently recovers the low level of albumin and other proteins that normally escape the glomerular filtration barrier. Two large receptors, megalin and cubilin/amnionless (CUBAM), bind to and efficiently retrieve these predominantly low molecular‐weight proteins via clathrin‐mediated endocytosis. Studies in cell culture models suggest that PT cells may sense changes in shear stress to modulate recovery of filtered proteins in response to normal variations in filtration rate. Impairments in PT endocytic function lead to the excretion of filtered proteins into the urine (tubular proteinuria). Remarkably, when the glomerular filtration barrier is breached, the PT is able to recover excess albumin with a capacity that is orders of magnitude higher than normal. What mediates this excess capacity for albumin uptake under nephrotic conditions, and why doesn't it compensate to prevent tubular proteinuria? Here we propose an integrated new working model to describe the PT recovery of filtered proteins under normal and nephrotic states. We hypothesize that uptake via the fluid phase provides excess capacity to recover high concentrations of filtered proteins under nephrotic conditions. Further, concentration of tubular fluid along the tubule axis will enhance the efficiency of uptake in more distal regions of the PT. By contrast to cells where fluid phase and receptor‐mediated uptake are independent pathways, expression of megalin is required to maintain apical endocytic pathway integrity and is essential for both uptake mechanisms. This model accounts for both the high‐affinity and the high‐capacity responses to filtration load in physiological and pathological states.
SUS-30 years: right and access in a day in the life of Primary Health Care.

OBJECTIVES to understand access in a day in the life Primary Health Care as a fundamental right to health, from users' perspective. METHODS a holistic-qualitative multiple case study based on Comprehensive Sociology of Everyday Life. 77 Unified Health System (SUS) users participated in the study. RESULTS according to users' voice, the right to health is still a matter of struggle, in more than 30 years of SUS. SUS is considered as the place where they have to appeal. The experiences are diverse, from the ease to the difficulty of access, neglecting users' needs, but despite the inequality in relation to the offer of services, the solution always arrives. FINAL CONSIDERATIONS to overcome this historical obstacle of comprehensive access to health, as a fundamental human right, it is necessary to overcome political and administrative decisions that hinder the construction of legitimate SUS, in a truly democratic participation of all social actors.
Attention-Based Meta-Reinforcement Learning for Tracking Control of AUV With Time-Varying Dynamics

Reinforcement learning (RL) is a promising technique for designing a model-free controller by interacting with the environment. Several researchers have applied RL to autonomous underwater vehicles (AUVs) for motion control, such as trajectory tracking. However, the existing RL-based controller usually assumes that the unknown AUV dynamics keep invariant during the operation period, limiting its further application in the complex underwater environment. In this article, a novel meta-RL-based control scheme is proposed for trajectory tracking control of AUV in the presence of unknown and time-varying dynamics. To this end, we divide the tracking task for AUV with time-varying dynamics into multiple specific tasks with fixed time-varying dynamics, to which we apply meta-RL for training to distill the general control policy. The obtained control policy can transfer to the testing phase with high adaptability. Inspired by the line-of-sight (LOS) tracking rule, we formulate each specific task as a Markov decision process (MDP) with a well-designed state and reward function. Furthermore, a novel policy network with an attention module is proposed to extract the hidden information of AUV dynamics. The simulation environment with time-varying dynamics is established, and the simulation results reveal the effectiveness of our proposed method.
Subliminal temporal integration of linguistic information under discontinuous flash suppression

Whether unconscious complex visual information integration occurs over time remains largely unknown and highly controversial. Previous studies have tended to use a combination of strong masking or suppression and a weak stimulus signal (e.g., low luminance), resulting in a low signal-to-noise ratio during unconscious stimulus presentation. To lengthen the stimulus exposure, we introduced intermittent presentation into interocular suppression. This discontinuous suppression allowed us to insert a word during each suppression period and deliver multiple words over time unconsciously. We found that, after participants received the subliminal context, they responded faster to a syntactically incongruent target word in a lexical decision task. We later replicated the finding in a separate experiment where participants exhibited chance performance on locating the subliminal context. These results confirmed that the sentential context was both subjectively and objectively subliminal. Critically, the effect disappeared when the context was disrupted by presenting only partial sentences or sentences with a reversed word order. These control experiments showed that the effect was not merely driven by word–word association but instead required integration over multiple words in the correct order. These findings support the possibility of unconscious high-level, complex information integration.
Sources of variation at the onset of bilingualism: The differential effect of input factors, AOA, and cognitive skills on HL Arabic and L2 English syntax

Abstract Despite growing research on individual differences in child bilinguals, few studies have focused on the development of syntax, included both languages, and studied newly arrived school-age migrant children. Accordingly, this study investigated the syntactic development of heritage language (HL) Syrian Arabic and L2 English by Syrian refugee children (N = 119) recently arrived in Canada using a sentence repetition task. Regression analyses showed that a partially overlapping set of child-level (input and cognitive skills) and language-level (syntactic structure) factors accounted for performance in each language. HL performance was particularly sensitive to language, cognitive, and input variables indexing cumulative HL exposure. L2 performance, however, was sensitive to cognitive and environmental variables indexing current and cumulative L2 use. Finally, despite stronger performance in Arabic than in English, results revealed interdependence between the two languages, indicating that participants with stronger syntactic abilities in their HL tended to have stronger syntactic abilities in their emerging L2.