Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of Minor N-linked Glycans on Antibodies Using Endo H Release and MALDI–Mass Spectrometry

Abstract A MALDI mass spectrometry method using Bruker Daltonic's LIFT technology for MS/MS analysis has been developed for profiling and characterizing low abundant N-glycans from recombinant immunoglobulin G (IgG) antibodies. In this method, Endoglycosidase H (Endo H) released N-glycans are derivatized at their reducing end with 2-aminobenzamide (2-AB) and separated by normal phase chromatography. Endo H hydrolyses the bond between the two GlcNAc residues of the trimannosyl core of high mannose and hybrid N-linked glycans, leaving the core GlcNAc attached to the protein. High mannose and hybrid type N-glycans are released from the glycoprotein whereas the more abundant, complex biantennary type oligosaccharide structures are unaffected. Analysis of Endo H treated glycan moieties by MALDI mass spectrometry identified several minor species of high mannose and hybrid type glycans. Subsequent MALDI TOF MS/MS analysis of the resulting products yielded information about structural features of the high mannose and hybrid type glycans. This study involving Endo H treatment followed by MALDI mass spectrometry coupled with LIFT technology for MS/MS analysis offers a specific and sensitive technique for visualizing, and characterizing minor glycan species.
LEGENDRE COLLOCATION METHOD FOR LINEAR SECOND ORDER FREDHOLM VOLTERRA INTEGRO-DIFFERENTIAL EQUATIONS

This paper discusses the development of a new numerical solution of second order linear Fredholm Volterra integro-dffierential equations by Legendre collocation method. The Fredholm Volterra integro-differential equation is rst converted into integral equation and then transformed into linear algebraic equations which are then solved using matrix inversion method. Numerical solution shows that the method gives better accuracy than the existing methods.
Non-fragile exponential polynomial observer design for a class of nonlinear fractional-order systems with application in chaotic communication and synchronisation

ABSTRACT This paper addresses the design of a non-fragile exponential polynomial observer for a class of fractional-order nonlinear systems. Existence of the observer is proven and a sufficient condition for the stability of the state estimation error dynamics with a predetermined exponential convergence rate is derived employing the Lyapunov stability theorem. The exponential stability criterion is proposed in the form of linear matrix inequalities (LMIs). Moreover, some numerical examples have been provided to illustrate the effectiveness of the proposed approach. The synchronisation of fractional-order Lorenz systems has been investigated using the proposed method. Then, the proposed method has been applied to the chaotic communication problem of fractional-order chaotic systems with four scroll attractors.
Two-level games and market constraints on politics in Europe

Financial markets understood the Euro crisis as a two-level game. They monitored national politics as a source of both national and European policy. The incentives to conform to the market’s preference were weaker for creditor countries than for debtor countries because debtors were providers of their own macroeconomic policy, but each creditor was one of several contributing to bailouts. Worries about default caused investors to sell the bonds of debtors and thereby constrained debtors by raising interest rates. By contrast, if creditor behaviour reduced the probability of a bailout of debtors, the response again would be to sell assets linked to the debtor. The implication is that market responses to creditor elections should have been larger and more turbulent than reactions to debtor elections. We test this theory by analyzing credit default swaps of eleven countries around fifteen elections and conducting a content analysis of 3,126 reports from Bloomberg terminals.
Comparative Developmental and Reproductive Biology of Three Populations of Propylea quatuordecimpunctata (Coleoptera: Coccinellidae)

Abstract Comparative studies showed no measurable differences among three populations of Propylea quatuordecimpunctata L. (Coleoptera: Coccinellidae) in their developmental and reproductive responses to temperature or photoperiod. Populations of P. quatuordecimpunctata from Canada, France, and Turkey reared on a diet of pea aphids, Acyrthosiphon pisum (Harris), at constant temperatures from 14 to 30°C required approximately 212 degree days (DD) > 10°C to complete preimaginal development. Survival of the three populations generally was highest at 22 and 26°C. On the basis of rearing under five constant-temperature conditions, the Turkish population produced the lowest percentage females (39%), whereas females of the Canadian population constituted the highest percentage (51%). No significant differences were observed among the three populations in the critical photoperiod (ca. L:D 15:9) for diapause induction at 22°C. A comparison of life table parameters, at 26°C, showed that the intrinsic rates of increase ( r m ), total progeny produced, preoviposition period, and net reproductive rates did not differ among the three populations.
Photosynthetic response and transcriptomic profiling provide insights into the alkali tolerance of clone halophyte Leymus chinensis

Alkali stress is one of the important factors in restricting agriculture production. Leymus chinensis is constructive halophyte species in alkalized grassland in China. In order to investigate the gene expression response of L. chinensis to alkali stress, we used PacBio technology to obtain reference full-length transcript sequences for transcriptomic analysis of alkali stress response. In order to elucidate the alkali tolerance mechanisms of L. chinensis, we measured the photosynthetic parameters, concentrations of ions and compatible solutes, chloroplast ultrastructure and anatomy of control and stressed plants. Our results showed that L. chinensis shares many alkali-tolerance mechanisms with glycophytes. Higher stability of photosynthetic apparatus under alkali stress may be prominent alkali-tolerance trait of L. chinensis. L. chinensis may have a strong capacity to decline the toxicity of Na+ to organelles and cytoplasmic proteins. Enhanced expression of dehydrin and LEA genes and increased accumulation of carbohydrates may contribute to the development of Na+-specific stress tolerance of L. chinensis under alkali stress.
Germinação de sementes osmocondicionadas e não osmocondicionadas e crescimento inicial de Physalis angulata L. (Solanaceae) em ambientes salinos

O genero Physalis pertencente a familia Solanaceae, possui 110 especies, das quais 11 ocorrem no Brasil. Physalis angulata L. destaca-se no genero pela presenca de vitaesteroides, principalmente as fisalinas, encontradas em raizes, caules e folhas, com propriedades imunomoduladora, antimicrobiana e anticancerigena. Este trabalho teve por objetivo avaliar a germinabilidade de sementes osmocondicionadas e nao osmocondicionadas e o crescimento inicial de P. angulata em ambientes salinos. Foram realizados experimentos nos quais as sementes foram submetidas ao osmocondicionamento (PEG 6000) e colocadas para germinar em solucoes com diferentes concentracoes de NaCl (2, 4, 6, 10, 12, 14 e 16 dS m-1). O controle (0 dS m-1) foi realizado com agua destilada. Os parâmetros avaliados foram a germinabilidade das sementes, o indice de velocidade de germinacao das sementes (IVG), o coeficiente de uniformidade de germinacao e a massa seca total das plântulas. Houve diminuicao da germinabilidade e do IVG, em ambos os tratamentos. No entanto, as sementes osmocondicionadas apresentaram maior tolerância ao estresse salino. Apesar da reducao significativa da massa seca em relacao ao aumento da salinidade do meio, o osmocondicionamento como tratamento pre-germinativo poderia ser utilizado para favorecer a tolerância ao estresse salino, uma vez que a germinabilidade foi maior para sementes osmocondicionadas.
Study on lightning protection scheme of multi‐terminal MMC‐MVDC distribution system

Based on a ±10 kV three-terminal MMC-MVDC (medium voltage DC distribution system based on modular multilevel converter) system, in this study, the authors mainly study on the influencing factors and protection schemes of the lightning intruding overvoltage (including direct lightning and back-flashover lightning). Firstly, a lightning intruding overvoltage simulation model of the MMC-MVDC distribution system using overhead lines is established based on the PSCAD/EMTDC program. The influence of steady-state operating voltage and the number of converter stations on the simulation results are simulated and analysed. Meanwhile, the proper simulation modelling method of the lightning intruding overvoltage for multi-terminal MMC-MVDC system is proposed. Secondly, the lightning intruding overvoltage is simulated when the line is struck by the lightning stroke current that occurs once in 20 years and the different lightning protection schemes are proposed. Finally, based on the lightning impulse withstand voltage, lightning trip-out rate and the cost of the protection schemes, the lightning protection schemes in different scenarios are proposed. The simulation results show that if the cost of the system is prioritised, the protection scheme of increasing the number of parallel stacks of the DL arresters can be selected, and if the power supply reliability of the system is prioritised, the protection scheme of installing the overhead ground wire as well as reducing grounding resistance can be selected.
Amino Acid Supplementation Improves the Production of Extracellular Peptidases by Aspergillus Section Flavi and their Ionic Immobilization

: Bioprocess studies have been highlighted due to the importance of physiological processes and industrial applications of enzymes. The potential of peptidase production from Aspergillus section Flavi using different amino acids as a supplemental reversibly immobilized on ionic supports, DEAE-Agarose and MANAE-Agarose the derivative showed different profiles of thermal and pH stabilities. Hence, this study revealed the basic properties and biochemical characteristics that allowed the production improvement of this class of enzyme. Moreover, with known properties stabilization and immobilization process is required to further explore its biotechnological capacities.
Software Reliability for Agile Testing

It is known that quantitative measures for the reliability of software systems can be derived from software reliability models, and, as such, support the product development process. Over the past four decades, research activities in this area have been performed. As a result, many software reliability models have been proposed. It was shown that, once these models reach a certain level of convergence, it can enable the developer to release the software and stop software testing accordingly. Criteria to determine the optimal testing time include the number of remaining errors, failure rate, reliability requirements, or total system cost. In this paper, we present our results in predicting the reliability of software for agile testing environments. We seek to model this way of working by extending the Jelinski–Moranda model to a “stack” of feature-specific models, assuming that the bugs are labeled with the features they belong to. In order to demonstrate the extended model, two use cases are presented. The questions to be answered in these two cases are: how many software bugs remain in the software and should one decide to stop testing the software?
What Is the Source of Occupational Stress and Burnout?

The purpose of this research was to examine the differences between the two genders in terms of occupational stress and burnout, as well as the effect of years of service on 141 Primary Education teachers. The degree of correlation between stress and burnout was also examined. A demographic questionnaire, teachers’ emotional stress questionnaire and teachers’ burnout questionnaire were used for data collection. The results of the study showed that there were no differences between men and women in terms of work stress and burnout. It has also been shown that years of service do not affect the degree of stress and burnout experienced by teachers and are not associated with feelings of exhaustion, depersonalisation, and personal achievement. However, according to the correlation analysis, a statistically significant relationship between work stress and occupational burnout was observed. The findings of this study are in agreement with relevant studies and can be used for early recognition and more effective occupational coping and treatment.
Rhizobium rhizogenes-mediated transformation of Rhodiola rosea leaf explants

: Rhodiola rosea L. is an endangered medicinal plant distributed in mountains and in high latitude regions. For its conservation, sustainable methods for the obtaining of its bioactive compounds must be developed. This work hypothesized that leaf, stem and rhizome explants of R. rosea from different geographical origins respond differently to inoculation with Rhizobium rhizogenes agropine strain ATCC43057. The objective was to generate R. rosea hairy roots (HRs) containing rol -genes. These HRs could be cultivated under axenic conditions for the extraction of the medical compounds rosavinoids and salidroside. Hereby, production of bioactive compounds could be improved per plant biomass. Thirteen R. rosea accessions of Alpine, Scandinavian, Nordic Gene Bank (NGB) and Russian origins were compared for their explant survival and HR formation. Significant differences were observed among plants from different geographical origins, where the NGB leaf explants exhibited up to 70% of HR formation and the Russian accessions did not exhibit HRs at all. Moreover, maintaining explants in light conditions after R. rhizogenes inoculation resulted in higher explant survival and HR formation rate (35%) when compared with explants kept in darkness (9%). Taken together, an efficient HR formation in roseroot by inoculation of R. rhizogenes following culturing in light was reported as a required step. This work represents a stepping-stone to R. rosea HR cultivation in bioreactors as well as regenerating whole plants. Hence, it is initiating a novel route towards high-throughput production of bioactive compounds as well preventing depletion of natural roseroot populations. following with . The effect of different from thirteen accessions of this from the and the and explants of rosea from different geographical differently with
The impact of brand familiarity, customer brand engagement and self-identification on word-of-mouth

PurposeThe purpose of this paper is to check for the effects of brand familiarity, customer brand engagement and self-identification on word-of-mouth (WOM) communication.Design/methodology/approachA systematic review of the literature regarding brand familiarity and customer brand engagement CBE) was conducted and data were analyzed using structural equation modeling.FindingsThe results revealed that brand familiarity had a positive impact on CBE; self-identification also had a positive impact on WOM communication.Research limitations/implicationsThe model was tested in the context of service sector; future research may investigate in different context.Practical implicationsThe framework advances insight into customer engagement and service dominant logic, which, despite having been recognized for their significant theoretical fit, have remained largely disparate in the literature.Originality/valueThis study is among the first few attempts to examine the impact of brand familiarity on different dimensions, namely, cognitive, affective and activation dimensions of CBE. This study contributes to a more detailed description of the brand familiarity construct and improves understanding of WOM communication. The study provides implications for practitioners and marketers.
A Framework for Total Quality Management of Diesel Generator Fuel Consumption using Machine Learning and Internet of Things (IoT)

Decision making on quantity of fuel consumption requirement is playing a very important role in industrial applications, for establishment of the production process which became more complicated and essential especially in Arab countries which have major shortage of fuel availability and price fluctuation and subsequently, Decision Making becomes very hard. Over the years, most of decisions were generated, based on personal experience which may not be effective due to many parameters such as level of experience of decision making and the state of the production system. Developing the ability to predict
Impact of direct drilling and conventional tillage on seasonal changes of soil water content in Chromic Haploxerert (south-west Spain)

A continuous monitoring of spatial and temporal variability of soil water content was studied under two soil management systems: direct drilling (DD) and conventional tillage (CT), during four consecutive seasons. The soil water content was read at different soil depths using multisensor capacitance probes in each soil-management treatment. During the first season (2003–2004), soil water content dynamics for both treatments were similar during the rainy season, although the DD plot was able to retain more water in the soil profile, and during the maximum evapotranspirative period faster soil water depletion took place in the CT plot. The 2004–2005 season registered a high evapotranspiration rate (1741 mm) with low rainfall (228 mm), promoting a low soil water recharge for both treatments. The 2005–2006 season registered an increase in rainfall, promoting a greater recovery of the soil water reserve in DD than CC plot. Finally, during the 2006–2007 season with an evapotranspiration rate ET0 and rainfall of 1504 and 560 mm, respectively, DD retained more soil water content, mainly in the deeper zones, with progressive soil water depletion during the maximum evapotranspirative period in comparison to CT. Thus, DD was demonstrated to be a promising soil management technique for improving the soil water content.
Development of freezing tolerance in roots and shoots of Scots pine seedlings at nonfreezing temperatures

The hardening of hydroponically cultured Scots pine (Pinus sylvestris L.) seedlings and their recovery after freezing was studied at the end of the second growing season (LD), after 3 weeks of short day treatment (SD), after a gradual decrease in temperature to 5°C over 4 weeks (H1), and after 4 weeks at 5°C (H2). Frost hardiness was determined by several methods and the recovery as survival of the seedlings. The highest frost hardiness was achieved in the distal parts of needles (-21 to -27°C) and in the proximal parts of needles (-18 to -25°C), followed by woody roots (-7 to -9°C), the 1-year-old and current stem (-8°C), and the fine roots (-5°C), all at the end of H2. Hardening of needles was induced by SD, but the stem and woody roots started to harden later, as a response to low temperature. As a result of frost treatment during LD and SD, potential plasma membrane roman H+-ATPase activity of roots decreased as electrolyte leakage increased and extracellular resistance decreased, but this relationshi.
The ecology and feeding biology of two Polydora species (Polychaeta: Spionidae) found to ingest the embryos of host hermit crabs (Anomura: Decapoda) from the Philippines

The ecology and feeding biology of two shell-burrowing polydorids associated with hermit crabs from the Philippines were investigated. Polydora umangivora and P. robi were found in shells of 15 and 28 gastropod species, respectively, occupied by nine hermit crab species. Polydora umangivora constructs U-shaped burrows in the outer shell while P. robi constructs burrows in the apex of the shell, extending to an opening along the columella of the upper body whorls. Polydora umangivora and P. robi preyed upon the embryos of three and seven species, respectively, of diogenid hermit crabs collected in the field. In laboratory feeding trials, P. robi ingested up to 230 developing eggs and 70 embryos of Calcinus gaimardii over a 6-h period. The impact of embryo predation is more apparent in hermit crab species that produce small broods of eggs ( Paguristes runyanae ) than in species that produce large broods of eggs ( Calcinus gaimardii ). Polydora robi transports the embryos to the mouth by a combination of muscular and ciliary action of the palps; ingestion follows breakage of the attachment stalk between embryo and pleopod seta. Invertebrates known and suspected to feed on host hermit crab eggs are reviewed and the evolution of polydorid/hermit crab symbioses is discussed.
THE ROLE OF PREDICTOR PLACENTAL GROWTH FACTOR, SOLUBLE ENDOGLIN, SOLUBLE-FMS-LIKE-TYROSINE KINASE-1 AND PULSATIL UTERINA ARTERIAL INDEX TO PREDICT THE EARLY ONSET OF PREECLAMPSIA

Objective: In preeclampsia, there will be an increase in SFlt-1 and in and decrease in PlGF levels. This condition will cause disorders of vasculogenesis and angiogenesis in fetomaternal circulation, which will eventually lead to preeclampsia syndrome such as proteinuria, hypertension and endothelial dysfunction. Methods: An observational study design with nested case-control. The study was conducted at Bunda Thamrin Hospital, Tanjung Mulia Medika Hospital, Sundari Hospital and private practice, from March to November 2018, with a sample of 64 research subjects. Results: The results of this study indicate that there were significant values with p<0.05, namely the pulsatile value of the uterine artery index with a 1.228 cut-off point, Area Under Curve (AUC) of 78.2% (95% CI 59.3%-97%), sensitivity 80%, specificity 64.6%, PlGF level with 441 pg/ml cut-off point, Area Under Curve (AUC) of 82.5% (95% CI 61.5%-100%), sensitivity 80%, specificity 87.7%, sFlt-1 level with a cut-off point of 10087.5 pg/ml, Area Under Curve (AUC) of 81.2% (95% CI 63.6%-98.9%), sensitivity 80%, specificity 67.7% while sEng with p value>0.05 which means it is not significant. Conclusion: From this study, no significant differences were found in sEng, whereas differences were found in the pulsatile value of the uterine artery index, PlGF levels, and sFlt-1 levels in the incidence of early-onset preeclampsia. From the multivariate analysis, an examination of PlGF levels alone is sufficient as a predictor of early-onset preeclampsia.
Design and implementation of speed fluctuation reduction for a ball screw feed system at low-speed operation

Abstract. In the high-precision servo feed system, when the permanent magnet synchronous motor (PMSM) is operated at low speed in the classical drive feed system (CDFS), the speed fluctuation caused by the motor torque harmonics seriously affects the speed smoothness of the servo system. In this paper, a novel double-drive differential feed system (DDFS) is proposed to effectively suppress the effect of torque harmonics of PMSM on speed fluctuation of the linear feed system at low-speed operation. Firstly, the effect of motor torque harmonics on motor speed for the DDFS is analyzed by the sensitivity function of the servo system, which indicates that the torque harmonics have little effect on the motor speed at high-speed operation. Then, in the DDFS, we make two motors rotate in the same direction at high speed and differentially synthesize at the ball screw to obtain low-velocity linear motion. Compared with the CDFS, the DDFS can suppress the effect of motor torque harmonics on speed fluctuation of the table and improve speed smoothness at low-speed operation.