Miracle-dz/UniFAR-FTUs huggingface dataset:
Sustainable Maintenance of Connected Dominating Set by Solar Energy Harvesting for IoT Networks

A new scheme, Sustainability in Dominating Set (SID), to construct and maintain a sustainable and adaptive node disjoint weighted connected dominating set (CDS) is presented for solar energy harvesting-enabled Internet of Things (IoT) networks. The primary contribution of this work is the design of an approximation algorithm for CDS construction and maintenance in energy harvesting-enabled IoT networks, which is triggered when energy of any CDS node falls below a threshold. This is a new CDS construction approach of approximation ratio <inline-formula> <tex-math notation="LaTeX">$\frac {\delta w(G) + 1}{2} + (1 + \ln 5) |O_{p}|$ </tex-math></inline-formula>, where <inline-formula> <tex-math notation="LaTeX">$\delta w(G)$ </tex-math></inline-formula> and <inline-formula> <tex-math notation="LaTeX">$|O_{p}|$ </tex-math></inline-formula> are weighted average degree of the network and optimal number of Steiner nodes, respectively. The extensive simulation results show that per-slot-rotation-based SID (SIDS) improves residual energy (saves by 14.3%, 25.6%, 8.25% and 8%), message complexity (reduces by 88.5%, 34% and 72.75% and 83.25%), and average CDS size (reduces by 9.01%, 9.09%, 41.75%, and 54.5%) for two scenarios and cloud cover 0.25, 0.5 and 0.8, compared to LMW, Improved LMW (ILMW), DataCDS and UCDS, respectively. Merely based on threshold switching, SID enhances residual energy, message complexity, and CDS size by 3.08%, 51.88%, and 4% for all scenarios and cloud covers compared to SIDS, respectively.
Soluble Papain to Digest Monoclonal Antibodies; Time and Cost-Effective Method to Obtain Fab Fragment

Antigen binding fragments (Fabs) used in research (e.g., antibody mimetics, antibody-drug conjugate, bispecific antibodies) are frequently obtained by enzymatic digestion of monoclonal antibodies using immobilised papain. Despite obtaining pure Fab, using immobilised papain to digest IgG has limitations, most notably slow digestion time (more than 8 h), high cost and limited scalability. Here we report a time and cost-effective method to produce pure, active and stable Fab using soluble papain. Large laboratory scale digestion of an antibody (100 mg) was achieved using soluble papain with a digestion time of 30 min and isolated yields of 55–60%. The obtained Fabs displayed similar binding activity as Fabs prepared via immobilised papain digestion. Site-specific conjugation between Fabs and polyethylene glycol (PEG) was carried out to obtain antibody mimetics FpF (Fab-PEG-Fab) indicating that the native disulphide bond had been preserved. Surface-plasmon resonance (SPR) of prepared FpFs showed that binding activity towards the intended antigen was maintained. We anticipate that this work will provide a fast and less costly method for researchers to produce antibody fragments at large scale from whole IgG suitable for use in research.
Somatic Mobilization: High Somatic Insertion Rate of mariner Transposable Element in Drosophila simulans

Simple Summary Transposable elements are capable of promoting somatic mobilization, and this may potentially have biological consequences. However, the rate of somatic mobilization is poorly known. The mariner element causes somatic mobilization in Drosophila simulans. We used an assay based on sequencing of the flanking mariner region and developed a pipeline to identify new mariner insertions in D. simulans. We found that flies carrying two mariner copies (one autonomous and one non-autonomous) had approximately 300 new somatic insertions during fly development (eggs to adult). We show that the rate of somatic insertion of the mariner element in D. simulans is high. Abstract Although transposable elements (TEs) are usually silent in somatic tissues, they are sometimes mobilized in the soma and can potentially have biological consequences. The mariner element is one of the TEs involved in somatic mobilization (SM) in Drosophila and has a high rate of somatic excision. It is also known that temperature is an important factor in the increase of the mariner element SM in the fly. However, it is important to emphasize that excision is only one step of TE transposition, and the final step in this process is insertion. In the present study, we used an assay based on sequencing of the mariner flanking region and developed a pipeline to identify novel mariner insertions in Drosophila simulans at 20 and 28 °C. We found that flies carrying two mariner copies (one autonomous and one non-autonomous) had an average of 236.4 (±99.3) to 279 (±107.7) new somatic insertions at 20 °C and an average of 172.7 (±95.3) to 252.6 (±67.3) at 28 °C. In addition, we detected fragments containing mariner and others without mariner in the same regions with low-coverage long-read sequencing, indicating the process of excision and insertion. In conclusion, a low number of autonomous copies of the mariner transposon can promote a high rate of new somatic insertions during the developmental stages of Drosophila. Additionally, the developed method seems to be sensitive and adequate for the verification and estimation of somatic insertion.
Effects of Oat Beta-Glucan Intake on Lipid Profiles in Hypercholesterolemic Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

(1) Background: hyperlipidemia is one of the cardiovascular diseases which becomes a great threat to the health of people worldwide. Oat beta-glucan is reported to have a beneficial effect on lowering blood lipids. To probe the effect of oat beta-glucan consumption on serum lipid profiles (total cholesterol, total triglyceride, high-density lipoprotein-cholesterol, and low-density lipoprotein-cholesterol), we carried out a systematic search on randomized controlled trials of oat beta-glucan intervention on hypercholesterolemic individuals. (2) Methods: the pieces of literature were obtained from PubMed, Scopus, Cochrane Library, Web of Science, and the Embase from inception to 28 February 2022. The results were presented with the weighted mean difference (WMD) with a 95% CI. The random-effects or fixed-effects model was applied according to the heterogeneity. The subgroup analysis and meta-regression were used to identify the source of heterogeneity. (3) Results: thirteen trials with 927 participants were included in our meta-analysis. Overall, oat beta-glucan supplementation significantly reduced levels of TC (pooled WMD = −0.24 mmol/L; 95%CI: −0.28 to −0.20 mmol/L), LDL-c (pooled WMD = −0.27 mmol/L; 95%CI: −0.35 to −0.20 mmol/L). Furthermore, beta-glucan consumption did not show significant effects on TG (pooled WMD = −0.04 mmol/L; 95%CI: −0.13 to 0.05 mmol/L), HDL-c (pooled WMD = 0.00 mmol/L; 95%CI: −0.05 to 0.05 mmol/L). Subgroup analysis indicated that critical factors, such as disease severity of participants, the daily intervention of oat beta-glucan, source of oat beta-glucan, and duration of intervention had impacts on outcomes. (4) Conclusions: oat beta-glucan intake may significantly decrease the level of TC and LDL-c while no significant changes in TG and HDL-c were observed. This meta-analysis supports the health benefits of oat beta-glucan, especially for its cholesterol-lowering features, although it has some inevitable limitations.
RETRACTED ARTICLE: Torque Coordinated Control of Hybrid Power System Based on Feedback Iterative Algorithm in Industry 4.0

We, the Editor, Institution and Publisher of the journal IETE Journal of Research, have retracted the following article, which is part of the Special Issue titled “Federated Learning for Blockchain Systems and Industrial Internet of Things”: Yunlong Wang, Hongtian Zhang & Yuantao Sun (2022) Torque Coordinated Control of Hybrid Power System Based on Feedback Iterative Algorithm in Industry 4.0, IETE Journal of Research, DOI: 10.1080/03772063.2022. 2069605 Following publication, concerns were raised about the peer review and decision-making processes for this special issue. After an investigation by the Taylor & Francis Publishing Ethics & Integrity team, in full cooperation with the Editor-in-Chief and the Institution, it was confirmed that the articles included in this special issue were not reviewed appropriately, in line with the Journal's peer review standards and policy. As the stringency of the peer review process is core to the integrity of the publication process, the Editor and Publisher have decided to retract all of the articles within the above-named Special Issue. The journal has not confirmed if the authors were aware of this compromised peer review process. The journal is committed to correcting the scientific record and will fully cooperate with any institutional investigations into this matter. The authors have been informed of this decision. We have been informed in our decision-making by our editorial policies and the COPE guidelines. The retracted articles will remain online to maintain the scholarly record, but they will be digitally watermarked on each page as ‘Retracted’.
Influence of the Carbon and Nitrogen Sources on Diabolican Production by the Marine Vibrio diabolicus Strain CNCM I-1629

Recent advances in glycobiotechnology show that bacterial exopolysaccharides (EPS) presenting glycosaminoglycan (GAG)-like properties can provide a valuable source of bio-active macromolecules for industrial applications. The HE800 EPS, named diabolican, is a marine-derived anionic high-molecular-weight polysaccharide produced by Vibrio diabolicus CNCM I-1629 which displays original structural features close to those of hyaluronic acid. We investigated the impact of carbon and nitrogen substrates on both Vibrio diabolicus growth and diabolican production. Both substrates were screened by a one-factor-at-a-time method, and experimental designs were used to study the effect of glucose, mannitol, and ammonium acetate various concentrations. Results showed that the medium composition affected not only the bacterium growth and EPS yield, but also the EPS molecular weight (MW). EPS yields of 563 and 330 mg L−1 were obtained in the presence of 69.3 g L−1 glucose and 24.6 g L−1 mannitol, respectively, both for 116.6 mM ammonium acetate. MW was the highest, with 69.3 g L−1 glucose and 101.9 mM ammonium acetate (2.3 × 106 g mol−1). In parallel, the bacterial maximum specific growth rate was higher when both carbon and nitrogen substrate concentrations were low. This work paves the way for the optimization of marine exopolysaccharide production of great interest in the fields of human health and cosmetics.
The poly(A) polymerase PAPS1 mediates pollen maturation by regulating sperm cell differentiation in plants

Abstract In flowering plants, a haploid microspore undergoes an asymmetric division to produce the male germline that encounters a mitotic division to produce two germ cells. The resulting germ cells undergo a series of specialization events to produce the two sperm cells required for double fertilization. These events include to upregulate male germline‐specific while downregulating male germline‐nonspecific regulon, but how these specializations events are regulated, are still unresolved. To know how plant sperm cell is specialized, we mutagenized Arabidopsis double homozygous transgenic line (MGH3p‐MGH3::eGFP and ACTIN11p‐H2B::mRFP) by an ethyl methane sulfonate (EMS) treatment and isolated a mutant with sperms identity loss, resulting in a completely male defective plant. Second‐generation sequencing identified a point mutation G/A causing premature stop codon TGG/TGA in the poly(A) polymerase PAPS1 that is linked with phenotype. Further, we found that paps1 mutant fails to upregulate male germline‐specific regulon and to downregulate male germline‐nonspecific factors required for sperm cell differentiation and attaining pollen maturation. Previously, polyadenylation of pre‐mRNAs by PAPS1 has been found crucial for both RNA‐based silencing processes and the processing of pre‐mRNAs into mature mRNAs ready for translation. This study concludes that PAPS1 mediates sperm cell differentiation through upregulating specific while silencing the nonspecific factors of male germlines.
TITLE: A standardized approach for case selection and genomic data analysis of maternal exomes for the diagnosis of oocyte maturation and early embryonic developmental arrest in IVF. RUNNING: Identifying genetic variants in infertility

OBJECTIVE : To develop a methodology for case selection and whole-exome sequencing (WES) analysis in infertile women due to recurrent oocyte maturation defects(OOMD) and/or preimplantation embryo lethality (PREMBL). DESIGN : Retrospective cohort study. PATIENTS : A statistical methodology for identification of infertile endophenotypes (recurrent low oocyte maturation rate, LMR, low fertilization rate, LFR, and preimplantation developmental arrest, PDA, was developed using a large IVF dataset (11,221 couples). 28 OOMD/PREMBL infertile women were subsequently enrolled for WES. INTERVENTION : 30X-WES was performed on women’s gDNA. Pathogenic variants were prioritized using a custom-made bioinformatic pipeline set to minimize false positive discoveries through resampling in control cohorts (i.e., HGDP and 1,343 WES from oocyte donors). Individual scRNAseq data from 18 human MII oocytes and antral granulosa cells(AGC) was used for genome-wide validation. prioritization sample variants in CEP128 gene, participating in the formation of the spindle in mitosis and ciliogenesis. CONCLUSIONS : This analytical framework revealed known and new genes associated with isolated recurrent OOMD/PREMBL, providing essential indications for scaling this strategy to larger studies.
Open area 2 × 2 MIMO channel model for 2 GHz low‐elevation links with diversity and capacity applications

In civil surveillance applications, unmanned aerial vehicles (UAV) are being increasingly used in floods, fires, and law enforcement scenarios. In order to transfer large amounts of information from UAV‐mounted cameras, relays, or sensors, large bandwidths are needed in comparison to those required for remotely commanding the UAV. This demands the use of higher‐frequency bands, in all probability in the vicinity of 2 or 5 GHz. Novel hardware developments need propagation channel models for the ample range of operational scenarios envisaged, including multiple‐input, multiple‐output (MIMO) deployments. These configurations may enable a more robust transmission by increasing either the carrier‐to‐noise ratio statistics or the achievable capacity. In this paper, a 2 × 2 MIMO propagation channel model for an open‐field environment capable of synthesizing a narrowband time series at 2 GHz is described. Maximal ratio combining diversity and capacity improvements are also evaluated through synthetic series and compared with measurement results. A simple flat, open scenario was evaluated based on which other, more complex environments can be modeled.
Experimental Assessment of PV Panels Front Water Cooling Strategy

The electrical performance and reliability of flat- type photovoltaic (PV) modules can be severely affected by elevated cell operating temperature due to elevated ambient temperatures. In this work the free flow front water cooling solutions to enhance flat-type PV module electrical performance is experimentally explored on laboratory scale module operated outdoors under natural light luminance. Water-cooling is implemented using a perforated tube disposed on the top of the panel and a chilled water source, both attached to the module active surface. The cooling water directly wets the module active surface, thereby decreasing the light reflection loss and cleaning the panel surface in the same time. For given meteorological conditions, the efficiency of free water cooling was measured and the corresponding additionally electrical energy yield was determined. The equivalent electric circuit of the PV panel cooling system is developed and an appropriate algorithm for its parameters experimental determination is proposed.
Matchingand control study on driving system of full hydraulically-driven chassis

Pertaining to the working characteristics of construction vehicles,a special-purpose chassis,which is suitable for construction vehicles,is developed using the hydrostatic transmission technique for driving mode selection via road conditions.By analyzing the dynamic models,the required wheel property field is established.Meanwhile,the hydraulic system parameters are matched.Based on the precise control advantage of hydrostatic transmission,the construction vehicles can be precisely operated so as to enhance the vehicle driving capability through full-wheel driving.
Fast and Accurate Frequency Estimation for Renewable Energy Systems using Maximum Decay Sidelobes Windows

Fast and accurate signal frequency estimation has great importance i.a. in renewable energy systems and power control systems. The energy produced by these systems shall fulfill quality requirements as defined in the respective directives and standards. More accurate and faster grid signal frequency estimation (which can be used to control the inverter) can improve the energy quality. This article presents an overview of methods for spectrum interpolation and signal frequency estimation and a generalized method for very accurate and fast frequency grid estimation by using the Fast Fourier Transform procedure and maximum decay sidelobes windows. An important feature of this algorithm is the elimination of the impact of the conjugate component on the estimation result. The article shows the statistical properties of the frequency estimator as well as the effect of noise for the estimation. The results of the simulation show that the algorithm can be successfully used for a fast and accurate estimation of the grid signal frequency i.a. in renewable energy systems and power control systems. The accuracy of the frequency estimation is in the order of 5·10 -11 Hz for a 5 ms measurement window. Using different windows from the discussed family allows to adjust to the actual requirements of estimation.
di Easy Tuition

: ERP is a system that is system that need to implement in almost companies to growth the business, traditional companies inevitably have to keep up with the times, especially technology, otherwise, the company will be slow to develop their daily business, especially in managing company operations which is often as much problem for many companies in the present. Therefore, to answer these problems we do research that focuses on how to implement a good ERP system in organization, whether it’s in the field of developing systems that are integrated with all company operations to systems related to decision making. Nowadays, ERP systems are growing and has implemented a web base version that can be accessed in real time 24 hours a day anywhere and anytime. This research emphasizes the implementation of an ERP system in a company. this study uses the Research and Development or R&D method which is a very essential method to complement and update existing products that are useful as learning materials. The results of this study is to give the correct information for implementing an ERP system in a company based on world framework, thus, that every company would be understand how to manage company operations by IT system to help the company grow forward.
Mice 1 Repertoire in Inbred and F after Vaccinia Virus Infection and the Naive T Cell Immunodominance + Altered CD8

Previous studies of CD8 + T cell immunodominance after primary virus infection of F 1 mice compared with their inbred parents have generally concluded that no dramatic changes occur. In this study, we revisit this issue using vacciniavirus (VACV), which has a large genome, a recently deﬁned immunodominance hierarchy in mice, and is a candidate vector for vaccines. We found that immunogenicity of VACV peptides deﬁned using inbred mice was highly variable in F 1 progeny: some peptides were equally immunogenic in F 1 and inbred, whereas others elicited responses that were reduced by >90% in F 1 mice. Furthermore, the dominance of a peptide in the relevant inbredparent did not predict whether it wouldbe poorly immunogenic inF 1 mice. This result held usingF 1 hybrids of MHC-congenic mice, suggesting that MHC differences alone were responsible. It was also extended to foreign epitopes expressed by an rVACVvaccine.F 1 micewerelessabletomountresponsestothepoorlyimmunogenicpeptideswhenusedasasoleimmunogen,ruling out immunodomination. In addition, conserved TCR V b usage between inbred and F 1 mice did not always correlate with strong responses in F 1 mice. However, direct estimation of naive precursor numbers showed that these were reduced in F 1 compared with inbred mice for speciﬁcities that were poorly immunogenic in the hybrids. These data have implications for our understanding of the extent to which MHC diversity alters the range of epitopes that are immunogenic in outbred populations. The Journal of Immunology , 2010, 184: 45–55.
Evaluation of Green Residential Housing Market Maturity: Empirical Evidence from Nanjing, China

With the exacerbation of the human living environment and the clear increase in the public′s awareness of the need for energy saving, the Chinese government has increasingly been focusing upon the green housing market. The green housing market, however, is developing relatively slowly. The quality of the ″green″ houses being produced and the current levels of market supply and demand are under-developed. Therefore, this article aims to provide a more comprehensive understanding and evaluation of the green housing market and its degree of maturity in China. Authors achieve this understanding through system dynamics simulation results. Firstly, by analyzing the internal effects and interactions of demand subsystems, development subsystems, and government action subsystems, a system dynamics model (which is used to describe the green housing market) is established. Secondly, our study builds a maturity assessment model, which is based on the theory of maturity. In addition, our study dynamically conducts an evaluation of the development of the green housing market. Thirdly, our study combines the simulation results with the maturity evaluation, in order to achieve a dynamic evaluation and prediction of the development of the green housing market. Finally, this paper puts forward some policy suggestions.
PoKE: A Prompt-based Knowledge Eliciting Approach for Event Argument Extraction

Eliciting knowledge from pre-trained language models via prompt-based learning has shown great potential in many natural language processing tasks. Whereas, the applications for more complex tasks such as event extraction are less studied since the design of prompt is not straightforward for the structured event containing various triggers and arguments. % Meanwhile, current conditional generation methods employ large encoder-decoder models, which are costly to train and serve. In this paper, we present a novel prompt-based approach, which elicits both the independent and joint knowledge about different events for event argument extraction. The experimental results on the benchmark ACE2005 dataset show the great advantages of our proposed approach. In particular, our approach is superior to the recent advanced methods in both fully-supervised and low-resource scenarios.