Miracle-dz/UniFAR-FTUs huggingface dataset:
Downregulated miR-18a and miR-92a synergistically suppress non-small cell lung cancer via targeting Sprouty 4

ABSTRACT As a novel noncoding RNA cluster, miR-17-92 cluster include six members: miR-17, miR-18a, miR-19a, miR-19b-1, miR-20a, and miR-92a. Dysregulation of miR-17-92 has been proved to be connected with the advancement of a series of human diseases, but the roles of miR-17-92 cluster in non-small cell lung cancer (NSCLC) have not been absolutely elaborated. Herein, we determined that miR-17-92 cluster were upregulated significantly in NSCLC tissues, and the cell proliferation, migration and cycle progression of NSCLC were also facilitated under the function of miR-17-92 cluster. Sprouty 4 (SPRY4) was a direct target of miR-92a, and its overexpression restrained the exacerbation of NSCLC induced by miR-92a. Furthermore, the tumor xenograft assay showed that miR-92a facilitated tumor growth by inhibiting the expression of SPRY4 and mediating Epithelial-Mesenchymal Transition (EMT) in vivo. Finally, we looked into the synergistic effects of miR-92a and miR-18a on NSCLC, and found that antagomiR-18a treatment arrested the tumor growth rate of xenografted mice markedly. GRAPHICAL ABSTRACT
Pathway‐Specific Remodeling of Thalamostriatal Synapses in a Mouse Model of Parkinson's Disease

The network pathophysiology underlying the motor symptoms of Parkinson's disease (PD) is poorly understood. In models of late‐stage PD, there is significant cell‐specific remodeling of corticostriatal, axospinous glutamatergic synapses on principal spiny projection neurons (SPNs). Neurons in the centrolateral nucleus (CLN) of the thalamus that relay cerebellar activity to the striatum also make axospinous synapses on SPNs, but the extent to which they are affected in PD has not been definitively characterized.
Underwater acoustic target recognition method based on a joint neural network

To improve the recognition accuracy of underwater acoustic targets by artificial neural network, this study presents a new recognition method that integrates a one-dimensional convolutional neural network and a long short-term memory network. This new network framework is constructed and applied to underwater acoustic target recognition for the first time. Ship acoustic data are used as input to evaluate the network performance. A visual analysis of the recognition results is performed. The results show that this method can realize the recognition and classification of underwater acoustic targets. Compared with a single neural network, the relevant indices, such as the recognition accuracy of the joint network are considerably higher. This provides a new direction for the application of deep learning in the field of underwater acoustic target recognition.
Emerging therapies for human hearing loss

ABSTRACT Introduction More than 5% of the world’s population have a disabling hearing loss which can be managed by hearing aids or implanted electrical devices. However, outcomes are highly variable, and the sound perceived by recipients is far from perfect. Sparked by the discovery of progenitor cells in the cochlea and rapid progress in drug delivery to the cochlea, biological and pharmaceutical therapies are currently in development to improve the function of the cochlear implant or eliminate the need for it altogether. Areas covered This review highlights progress in emerging regenerative strategies to restore hearing and adjunct therapies to augment the cochlear implant. Novel approaches include the reprogramming of progenitor cells to restore the sensory hair cell population in the cochlea, gene therapy, and gene editing to treat hereditary and acquired hearing loss. A detailed review of optogenetics is also presented as a technique that could enable optical stimulation of the spiral ganglion neurons, replacing or complementing electrical stimulation. Expert opinion Increasing evidence of substantial reversal of hearing loss in animal models, alongside rapid advances in delivery strategies to the cochlea and learnings from clinical trials will amalgamate into a biological or pharmaceutical therapy to replace or complement the cochlear implant.
Detecting Prejudice From Egalitarianism: Why Black Americans Don’t Trust White Egalitarians’ Claims

Although White Americans increasingly express egalitarian views, how they express egalitarianism may reveal inegalitarian tendencies and sow mistrust with Black Americans. In the present experiments, Black perceivers inferred likability and trustworthiness and accurately inferred underlying racial attitudes and motivations from White writers’ declarations that they are nonprejudiced and egalitarian (Experiments 1 and 2). White writers believed that their egalitarianism seemed more inoffensive and indicative of allyship than was perceived by Black Americans (Experiment 1a). Linguistic analysis revealed that, when inferring racial attitudes and motivations, Black perceivers accurately attended to language emphasizing humanization, support for equal opportunity, personal responsibility, and the idea that equality already exists (Experiment 1b). We found causal evidence that these linguistic cues informed Black Americans’ perceptions of White egalitarians (Experiment 2). Suggesting societal costs of these perceptions, White egalitarians’ underlying racial beliefs negatively predicted Black participants’ actual trust and cooperation in an economic game (Experiment 3). Our experiments (N = 1,335 adults) showed that White Americans’ insistence that they are egalitarian itself perpetuates mistrust with Black Americans.
SAturation‐recovery and Variable‐flip‐Angle–based three‐dimensional free‐breathing cardiovascular magnetic resonance T1 mapping at 3 T

The purpose of the current study was to develop and validate a three‐dimensional (3D) free‐breathing cardiac T1‐mapping sequence using SAturation‐recovery and Variable‐flip‐Angle (SAVA). SAVA sequentially acquires multiple electrocardiogram‐triggered volumes using a multishot spoiled gradient‐echo sequence. The first volume samples the equilibrium signal of the longitudinal magnetization, where a flip angle of 2° is used to reduce the time for the magnetization to return to equilibrium. The succeeding three volumes are saturation prepared with variable delays, and are acquired using a 15° flip angle to maintain the signal‐to‐noise ratio. A diaphragmatic navigator is used to compensate the respiratory motion. T1 is calculated using a saturation‐recovery model that accounts for the flip angle. We validated SAVA by simulations, phantom, and human subject experiments at 3 T. SAVA was compared with modified Look‐Locker inversion recovery (MOLLI) and saturation‐recovery single‐shot acquisition (SASHA) in vivo. In phantoms, T1 by SAVA had good agreement with the reference (R2 = 0.99). In vivo 3D T1 mapping by SAVA could achieve an imaging resolution of 1.25 × 1.25 × 8 mm3. Both global and septal T1 values by SAVA (1347 ± 37 and 1332 ± 42 ms) were in between those by SASHA (1612 ± 63 and 1618 ± 51 ms) and MOLLI (1143 ± 59 and 1188 ± 65 ms). According to the standard deviation (SD) and coefficient of variation (CV), T1 precision measured by SAVA (SD: 99 ± 14 and 60 ± 8 ms; CV: 7.4% ± 0.9% and 4.5% ± 0.6%) was comparable with MOLLI (SD: 99 ± 25 and 46 ± 12 ms; CV: 8.8% ± 2.5% and 3.9% ± 1.1%) and superior to SASHA (SD: 222 ± 89 and 132 ± 33 ms; CV: 13.8% ± 5.5% and 8.1% ± 2.0%). It was concluded that the proposed free‐breathing SAVA sequence enables more efficient 3D whole‐heart T1 estimation with good accuracy and precision.
Adverse Childhood Experiences and Oral Health Conditions Among Middle-aged and Older Chinese Adults: Exploring the Moderating Roles of Education and Gender

This study aims to examine whether adverse childhood experiences (ACEs) are associated with oral health conditions (denture use, difficulty in chewing, and edentulism) among middle-aged and older adults in China and if gender and adulthood education moderate the associations. Data were obtained from the 2014 and 2018 surveys from the China Health and Retirement Longitudinal Study (N = 17,091) and logistic regressions were carried out. Results show that childhood hunger (OR = 1.12), loneliness (OR = 1.10) and family relations (OR = 1.07) were significantly associated with higher odds of denture use and there were significant associations between hunger (OR = 1.16) and difficulty in chewing. For the female subsample, education significantly moderated the adverse effect of childhood hunger on denture use and difficulty in chewing. Findings suggest that ACEs have long-lasting impacts on oral health conditions in later life and adulthood education might offer critical resources for females, helping them buffer the detrimental health impacts of ACEs.
The evidence behind the use of LASER for genitourinary syndrome of menopause, vulvovaginal atrophy, urinary incontinence and lichen sclerosus: A state‐of‐the‐art review

In recent years, LASER has been introduced as a minimally invasive treatment for a broad range of vaginal and vulvar symptoms and diseases. However, the efficacy and safety of vaginal and vulvar LASER has continuously been questioned. The aim of this study is to create an overview of the current literature and discuss the controversies within the use of LASER for genitourinary syndrome of menopause, vulvovaginal atrophy, urinary incontinence and lichen sclerosus. A search string was built in PubMed. The search was commenced on August 25, 2021 and closed on October 27, 2021. Two authors screened the studies in Covidence for inclusion according to the eligibility criteria in the protocol. The data were extracted from the studies and are reported in both text and tables. This review included 114 papers, of which 15 were randomized controlled trials (RCTs). The effect of LASER as a vaginal treatment was investigated for genitourinary syndrome of menopause in 36 studies (six RCTs), vulvovaginal atrophy in 34 studies (four RCTs) and urinary incontinence in 30 studies (two RCTs). Ten studies (three RCTs) investigated the effect of vulvar treatment for lichen sclerosus. Half of the included RCTs, irrespective of indication, did not find a significant difference in improvement in women treated with vaginal CO2 or Er:YAG LASER compared with their respective controls. However, most non‐comparative studies reported significant improvement after exposure to vaginal or vulvar LASER across all indications. Included studies generally had a short follow‐up period and only a single RCT followed their participants for more than 6 months post treatment. Adverse events were reported as mild and transient and 99 studies including 51 094 patients provided information of no serious adverse events. In conclusion, this review found that the effect of vaginal and vulvar LASER decreases with higher study quality where potential biases have been eliminated. We therefore stress that all patients who are treated with vaginal or vulvar LASER should be carefully monitored and that LASER for those indications as a treatment should be kept on a research level until further high‐quality evidence is available.
Diet or medication in primary care patients with IBS: the DOMINO study - a randomised trial supported by the Belgian Health Care Knowledge Centre (KCE Trials Programme) and the Rome Foundation Research Institute

Background In Europe, IBS is commonly treated with musculotropic spasmolytics (eg, otilonium bromide, OB). In tertiary care, a low fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAP) diet provides significant improvement. Yet, dietary treatment remains to be explored in primary care. We evaluated the effect of a smartphone FODMAP-lowering diet application versus OB on symptoms in primary care IBS. Methods IBS patients, recruited by primary care physicians, were randomised to 8 weeks of OB (40 mg three times a day) or diet and followed for 24 weeks. We compared IBS Symptom Severity Score and the proportion of responders (improvement ≥50 points) in all patients and the subgroup fulfilling Rome IV criteria (Rome+). We also evaluated treatment efficacy, quality of life, anxiety, depression, somatic symptom severity (Patient Health Questionnaire (PHQ15, PHQ9)) and treatment adherence and analysed predictors of response. Results 459 primary care IBS patients (41±15 years, 76% female, 70% Rome+) were randomised. The responder rate after 8 weeks was significantly higher with diet compared with OB (71% (155/218) vs 61% (133/217), p=0.03) and more pronounced in Rome+ (77% (118/153) vs 62% (98/158), p=0.004). Patients allocated to diet (199/212) were 94% adherent compared with 73% with OB (148/202) (p<0.001). The significantly higher response rate with diet was already observed after 4 weeks (62% (132/213) vs 51% (110/215), p=0.02) and a high symptom response persisted during follow-up. Predictors of response were female gender (OR=2.08, p=0.04) for diet and PHQ15 (OR=1.10, p=0.02) for OB. Conclusion In primary care IBS patients, a FODMAP-lowering diet application was superior to a spasmolytic agent in improving IBS symptoms. A FODMAP-lowering diet should be considered the first-line treatment for IBS in primary care. Trial registration number NCT04270487.
Association between hypertensive medication during pregnancy and risk of several maternal and neonatal outcomes in women with chronic hypertension: a population-based study

ABSTRACT Background Several studies have reported an association between perinatal complications and the severity of the hypertensive disease. The increasing number of pregnancies complicated by hypertension and the small assurance about the perinatal effects of hypertensive drug use during pregnancy involves the need of studying the better management of hypertensive mothers. Objective To evaluate the association between maternal use of antihypertensive drugs and maternal and neonatal outcomes in women with chronic hypertension. Study design We conducted a population-based study including all deliveries of hypertensive women that occurred between 2007–2017 in the Lombardy region, Italy. We evaluated the risk of several maternal and neonatal outcomes among women who filled antihypertensive prescriptions within the 20th week of gestation. Propensity score stratification was used to account for key potential confounders. Results Out of 5,553 pregnancies, 2,138 were exposed to antihypertensive treatment. With respect to no-users, users of antihypertensive drugs showed an increased risk of preeclampsia (RR:1.68, 95%CI:1.42–1.99), low birth weight (1.30,1.14–1.48), and preterm birth (1.25,1.11–1.42). These results were consistent in a range of sensitivity and subgroup analyses. Conclusion Early exposure to antihypertensive drugs in the first 20 weeks of gestation was associated with an increased risk of preeclampsia, low birth weight, and preterm birth.
Development and Evaluation of Internet-based Health Technology in Pediatric Oncology: A Scoping Review

Objective: There is an increased awareness to identify symptomatic experiences in children undergoing chemotherapy. An Internet-based health technology accessible and friendly for children and parents to report health problems during chemotherapy has been well-developed in developed countries. The purpose of this scoping review is to provide a comprehensive view of relevant research related to the emergence of health applications in pediatric oncology so that it can provide information for design and evaluation in the future. Methods: Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines support this scoping review. To investigate the evidence on the development of Internet-based health technology, Science Direct, Scopus, PubMed, EBSCOHOST Medline, IEEEXplore, ProQuest, and Nature databases were searched between 2015 and 2021. Results: 14 articles met the inclusion criteria with nine Internet-based health technologies. Moreover, four of nine mobile health apps use a theoretical foundation (SPARK for children and family member, Empower Stars! , THRIVE, and Facebook-based “Healthy Teens for Soaam”), three apps apply four stages of development and testing, and all apps have met the category of the degree of attachment of patients to the application. Conclusion: The effect of Internet-based health technology through a scientific process by paying attention to the underlying theories, user needs, developer passion, application testing, and evaluation methods is the key to success.
Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis

Abstract During meiosis, homologous chromosomes pair and recombine, which can result in reciprocal crossovers that increase genetic diversity. Crossovers are unevenly distributed along eukaryote chromosomes and show repression in heterochromatin and the centromeres. Within the chromosome arms, crossovers are often concentrated in hotspots, which are typically in the kilobase range. The uneven distribution of crossovers along chromosomes, together with their low number per meiosis, creates a limitation during crop breeding, where recombination can be beneficial. Therefore, targeting crossovers to specific genome locations has the potential to accelerate crop improvement. In plants, meiotic crossovers are initiated by DNA double-strand breaks that are catalyzed by SPO11 complexes, which consist of 2 catalytic (SPO11-1 and SPO11-2) and 2 noncatalytic subunits (MTOPVIB). We used the model plant Arabidopsis thaliana to coexpress an MTOPVIB-dCas9 fusion protein with guide RNAs specific to the 3a crossover hotspot. We observed that this was insufficient to significantly change meiotic crossover frequency or pattern within 3a. We discuss the implications of our findings for targeting meiotic recombination within plant genomes.
Dynamics of particle network in composite battery cathodes

Improving composite battery electrodes requires a delicate control of active materials and electrode formulation. The electrochemically active particles fulfill their role as energy exchange reservoirs through interacting with the surrounding conductive network. We formulate a network evolution model to interpret the regulation and equilibration between electrochemical activity and mechanical damage of these particles. Through statistical analysis of thousands of particles using x-ray phase contrast holotomography in a LiNi0.8Mn0. 1Co0.1O2-based cathode, we found that the local network heterogeneity results in asynchronous activities in the early cycles, and subsequently the particle assemblies move toward a synchronous behavior. Our study pinpoints the chemomechanical behavior of individual particles and enables better designs of the conductive network to optimize the utility of all the particles during operation. Description Observations of cathode evolution The loss of capacity in a rechargeable battery can be due to changes in the electrode structure that occur with cycling. Li et al. used hard x-ray holotomography to visualize the structure of a nickel-rich LiNi0.8Mn0. 1Co0.1O2 composite cathode (see the Perspective by Xiao). They were able to track the behavior of thousands of individual particles with time and thus determine the relationship between structure and performance as well as the deterioration of the cathode at a size scale that is not generally accessible. They found that damage during cycling is driven not only by each particle but also by its surrounding neighbors, although the contributions shift over time. This work suggests ways to better design electrodes to maximize their performance. —MSL Active particles in composite electrodes initially show asynchronous activity that evolves toward synchronous behavior.
Quantification methods for viruses and virus-like particles applied in biopharmaceutical production processes

ABSTRACT Introduction Effective cell-based production processes of virus particles are the foundation for the global availability of classical vaccines, gene therapeutic vectors, and viral oncolytic treatments. Their production is subject to regulatory standards ensuring the safety and efficacy of the pharmaceutical product. Process analytics must be fast and reliable to provide an efficient process development and a robust process control during production. Additionally, for the product release, the drug compound and the contaminants must be quantified by assays specified by regulatory authorities. Areas covered This review summarizes analytical methods suitable for the quantification of viruses or virus-like particles. The different techniques are grouped by the analytical question that may be addressed. Accordingly, methods focus on the infectivity of the drug component on the one hand, and on particle counting and the quantification of viral elements on the other hand. The different techniques are compared regarding their advantages, drawbacks, required assay time, and sample throughput. Expert opinion Among the technologies summarized, a tendency toward fast methods, allowing a high throughput and a wide applicability, can be foreseen. Driving forces for this progress are miniaturization and automation, and the continuous enhancement of process-relevant databases for a successful future process control.
ROS and Rosbridge

The advent of ROS, the Robot Operating System, has nally made it possible to implement and use state-of-the-art navigation and manipulation algorithms on widely-available, inexpensive standard robot platforms. With the addition of the Rosbridge application programming interface, interface designers and applications programmers can create robot interfaces and behaviors without venturing into the specialized world of robotics engineers. This tutorial introduces ROS and Rosbridge, and shows how quickly and easily these tools can be used to design and conduct large-scale online HRI experiments, access algorithms for autonomous robot behavior, and leverage the huge ecosystem of general-purpose web-based and application-oriented software engineering for robotics and HRI research. Tutorial attendees will learn the basics of autonomous and teleoperated navigation and manipulation, as well as interface design for online interaction with robots. During the tutorial they will design and write their own remote presence application, as well as develop strategies for incorporating autonomy and dealing with data collection.
Rendering and Simulation for Tires Rolling on Snow

approved: Yue Zhang Modeling tire-snow interaction is important in designing effective snow tires, which directly affects road safety during wintry weather. Unfortunately, tires have complex tread designs and the physical properties of snow have not been characterized. We employ the Material Point Method(MPM) for simulating a material that mimics the fracturing and plasticity of snow using established physical models. This realistic simulation allows us to assess the interactions between a breakable material like snow and a continuum structure like rubber tires, and determine the effect of tread patterns on tire traction. We also design a novel snow rendering technique for particle simulation using a combination of surface reconstruction, surface instanced geometry and sub-surface scattering. c ©Copyright by Alexander Clucas June 14, 2016 All Rights Reserved Rendering and Simulation for Tires Rolling on Snow