Miracle-dz/UniFAR-FTUs huggingface dataset:
The Thyroid Hormone Receptor- (TR ) Gene Encoding TR 1 Controls Deoxyribonucleic Acid Damage-Induced Tissue Repair

The thyroid hormone (TH) controls, via its nuclear receptor, TH receptor1 (TR 1), intestinal crypt cell proliferation in the mouse. In order to understand whether this receptor also plays a role in intestinal regeneration after DNA damage, we applied a protocol of -ray irradiation and monitored cell proliferation and apoptosis at several time points. In wild-type mice, the dose of 8 Gy induced cell cycle arrest and apoptosis in intestinal crypts a few hours after irradiation. This phenomenon reverted 48 h after irradiation. TR 0/0 mutant mice displayed a constant low level of proliferating cells and a high apoptosis rate during the period of study. At the molecular level, in TR 0/0 animals we observed a delay in the p53 phosphorylation induced by DNA damage. In our search for the expression of the protein kinases responsible for p53 phosphorylation upon irradiation, we have focused on DNA-dependent protein kinase catalytic subunit (DNA-PKcs). The number of cells expressing DNAPKcs in crypts remained high 48 h after irradiation, specifically in TR mutants. Altogether, in TR 0/0 animals the rate of apoptosis in crypt cells remained high, apparently due to an elevated number of cells still presenting DNA damage. In conclusion, the TR gene plays a role in crypt cell homeostasis by regulating the rate of cell renewal and apoptosis induced by DNA damage. (Molecular Endocrinology 22: 47–55, 2008)
Structuring Electronic Patient Record Data, a Smart Way to Extract Registry Information?

The paper reports from one of the first efforts to generate data for a national register by automatically reusing data recorded in the clinical documentation process. Today, the process of reporting to national registries implies filling out a paper-based or electronic form as the final step of documenting patients’ treatment. The registries’ forms are hence not part of the patients’ Electronic Patient Record (EPR). Therefore, the Norwegian Directorate for e-health has established a program for developing a shared infrastructure for 51 national registers, aiming to improve the utilization and quality of the reported health data. We argue that the quality of the registries’ data rests heavily on an understanding of today’s practice and how and when to capture the data. This paper describes the initial work of facilitating automatic reuse of standardized clinical data recorded from the EPR to the Norwegian registry for spine surgery. The empirical setting is the regional FRESK (Future systems in the clinic) program (2017-2022), in the North Norwegian Health Region.
Forest changes and fragmentation analysis of Hazaribagh district of Jharkhand, India using Geo-spatial technology

Forest cover and its fragmentation need to be assessed regularly for sustainable management of natural resources. In this study forest cover change and its fragmentation based on forest cover classifications derived from remotely sensed images. The study assesses the temporal changes of forest-cover maps from 1987 and 2019 using ArcGIS and FRAGSTATS. Visual interpretation technique involving on screen digitization was used for mapping and assesses the change statistics. In addition, FRAGSTAT was carried out to find the landscape matrix. Results showed that dense forest covers around 21.64% of study area in year 1987 which has decrease to become 18.90% in year 2019. It has also been observed that number of patches for dense forest increase from 116 to 226 and open forest increased from 161 to 446 between 1987 and 2019.This work demonstrates change in forest structure and pattern using geospatial technology which is cost effective and less time consuming.
Binding Sites for Hepatocyte Nuclear Factor 3b or 3g and Pancreas Transcription Factor 1 Are Required for Efficient Expression of the Gene Encoding Pancreatic a-Amylase

Efficient expression of genes under the control of a-amylase 2 5*-flanking sequences in exocrine pancreatic cells requires, in addition to the pancreas transcription factor 1 binding site (M. Cockell, B. J. Stevenson, M. Strubin, O. Hagenbüchle, and P. K. Wellauer, Mol. Cell. Biol. 9:2464–2476, 1989), another cis-acting element at positions260 to286. This DNA element, which contains an AT-rich core, constitutes a high-affinity binding site for nuclear proteins present not only in the pancreas but also in other tissues and cell lines derived from the endoderm. Purification of binding activities from pancreatic cells by DNA affinity chromatography reveals several distinct proteins ranging in size from 45 to 54 kDa (p45, p47/48, and p54). All of these proteins interact with the specific DNA sequence upon renaturation in vitro. Protein sequencing, electrophoretic mobility shift assay, and immunoblot analyses identify p54 and p47/48 as members of the hepatocyte nuclear factor 3 (HNF3 [forkhead]) family of transcription factors. p54 belongs to the subfamily of HNF3b proteins, while p47/48 binding activity includes HNF3g. The cDNAs for two HNF3b proteins differing only in N-terminal amino acid sequences were isolated from a pancreatic cDNA library. The mRNAs encoding the two protein species accumulate to different steady-state levels in poly(A) RNA of pancreatic cells. Our results support a model by which the pancreas-specific expression of the a-amylase gene is mediated by a combination of cell-specific and cell lineage-specific transcription factors.
CIA_A_260134 1449..1460

1Graduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil; 2Graduate Program in Physical Education, Federal University of Rio Grande do Norte, Natal, Brazil; 3Department of Physical Education, Federal University of Rio Grande do Norte, Natal, Brazil; 4Graduate Program in Psychobiology, Federal University of Rio Grande do Norte, Natal, Brazil Purpose: To investigate the short-term effect of self-selected training intensity (SSTI) on ambulatory blood pressure (BP) in hypertensive older women. Participants and Methods: This is a randomized, single-blind, two-arm, parallel-group controlled trial that included 40 medicated hypertensive older women (64.4±3.6 years; resting systolic 118±19 and diastolic BP 68±9 mmHg). SSTI intervention was performed three times per week, 30–50 minutes per session (n=20). The control group participated in health education meetings once per week (n=20). Ambulatory BP (primary outcome) and six-minute walking test performance (secondary outcome) were assessed at baseline and following 8 weeks of intervention. Heart rate (HR), rating of perceived exertion (RPE, 6–20), and affective valence (ie, feeling scale, −5/+5) were recorded during all SSTI sessions. Intention-to-treat and per-protocol analyses were used for data analyses. Results: Fifteen participants from the SSTI group and 17 from the control group completed the study. No differences in ambulatory BP (24-h, awake, and asleep) were observed between SSTI and control groups (intention-to-treat and per-protocol analyses; p>0.05). The SSTI group showed a greater six-minute walking test performance than the control group in the intention-to-treat and per-protocol analyses (p<0.05). The participants exercised at 52±10% of HR reserve reported an RPE of 11±1 and an affective valence of 3.4±1.1 over the 8-week period. Conclusion: SSTI is a feasible approach to induce a more active lifestyle and increase health-related fitness in hypertensive older women, although it does not improve BP control over a short-term period.
University of Birmingham CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV- transformed lymphoblastoid cell lines

Citation for published version (Harvard): Long, H, Haigh, T, Gudgeon, N, Leen, AM, Tsang, C, Brooks, J, Landais, E, Houssaint, E, Lee, S, Rickinson, A & Taylor, G 2005, 'CD4+ T-cell responses to Epstein-Barr virus (EBV) latent-cycle antigens and the recognition of EBV-transformed lymphoblastoid cell lines', Journal of virology, vol. 79, pp. 4896-4907. https://doi.org/10.1128/JVI.79. 8.4896-4907.2005, https://doi.org/10.1128/JVI.79. 8.4896-4907.2005
Title The Value of Media Studies Approaches for the Evaluation of Entertainment Education : A Case Study of East Los High . Permalink

As entertainment education (EE) becomes an increasingly popular medium for delivering health information, evaluation of EE is key to better understanding how it not only influences audiences but also how it can be better implemented. We suggest that EE evaluation has largely focused on impact while its implementation, specifically through narrative, characters, and genre, has remained unexplored. We propose drawing from theories and methods used in media studies to complement current approaches to understanding and evaluation of EE that can help improve EE for health education. East Los High serves as a case study, through which we examine the characters and the narrative demands of the telenovela genre to reveal a disproportional depiction of Latino men and women that ultimately replays familiar Latino stereotypes of criminality and exaggerated sexuality. We discuss the implications this has on the show’s ability to do public health work.
Characterising protein-protein interactions with the fragment molecular orbital method

1Evotec (UK) Ltd., 114 Innovation Drive, Milton Park, Abingdon, Oxfordshire OX14 4RZ, United Kingdom Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, 84538 Bratislava, Slovakia 3Research Department of Structural & Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, United Kingdom 4Research Center for Computational Design of Advanced Functional Materials (CD-FMat), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan
Pilot‐scale evaluation of nitrification control strategies

A significant concern associated with chloramination is nitrification caused by the growth of ammonia‐oxidizing bacteria (AOB) in the distribution system. To prevent nitrification, any measures designed to slow the decay of chloramine and its associated release of ammonia should theoretically reduce the occurrence of AOB growth. Pilot‐scale studies were used to evaluate whether enhanced removal of natural organic matter and pH adjustment delayed the onset of nitrification. For the system described in this article, nitrification occurred at pH values ranging from 7.9 to 8.9, with the time to onset of nitrification being fastest at about pH 8.5. However, pretreatment with enhanced coagulation successfully delayed the onset of nitrification compared with pretreatment with conventional coagulation. Nitrification did not occur when total chlorine concentrations were 2.2 mg/L as Cl2 or more. Similarly, nitrification did not occur in locations with biocide‐to‐food ratios of 1.9 mg Cl2/mg N or more.
Management of bakanae and bacterial seedling blight diseases in nurseries by irradiating rice seeds with atmospheric plasma

A. Ochi, H. Konishi, S. Ando, K. Sato, K. Yokoyama, S. Tsushima, S. Yoshida, T. Morikawa, T. Kaneko and H. Takahashi* Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 981-8555; Yamagata Integrated Agricultural Research Center, Yamagata, 990-2372; Department of Electronic Engineering, Graduate School of Technology, Tohoku University, Sendai, Miyagi, 980-8579; Yamagata Prefecture Plant Protection Office, Yamagata, 990-2372; Environmental Biofunction Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki, 305-8604; and Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, Tsukuba, Toyama, 939-8153, Japan
Phillips-Jones, Mary K. and Harding, Stephen E. (2018) Antimicrobial resistance (AMR) nanomachines: mechanisms for fluoroquinolone and glycopeptide

In this review, we discuss mechanisms of resistance identified in bacterial agents Staphylococcus aureus and the enterococci towards two priority classes of antibiotics—the fluoroquinolones and the glycopeptides. Members of both classes interact with a number of components in the cells of these bacteria, so the cellular targets are also considered. Fluoroquinolone resistance mechanisms include efflux pumps (MepA, NorA, NorB, NorC, MdeA, LmrS or SdrM in S. aureus and EfmA or EfrAB in the enterococci) for removal of fluoroquinolone from the intracellular environment of bacterial cells and/or protection of the gyrase and topoisomerase IV target sites in Enterococcus faecalis by Qnr-like proteins. Expression of efflux systems is regulated by GntR-like (S. aureus NorG), MarR-like (MgrA, MepR) regulators or a two-component signal transduction system (TCS) (S. aureus ArlSR). Resistance to the glycopeptide antibiotic teicoplanin occurs via efflux regulated by the TcaR regulator in S. aureus. Resistance to vancomycin occurs through modification of the D-Ala-D-Ala target in the cell wall peptidoglycan and removal of high affinity precursors, or by target protection via cell wall thickening. Of the six Van resistance types (VanA-E, VanG), the VanA resistance type is considered in this review, including its regulation by the VanSRTCS. We describe the recent application of biophysical approaches such as the hydrodynamic technique of analytical ultracentrifugation and circular dichroism spectroscopy to identify the possible molecular effector of the VanS receptor that activates expression of the Van resistance genes; both approaches demonstrated that vancomycin interacts with VanS, suggesting that vancomycin itself (or vancomycin with an accessory factor) may be an effector of vancomycin resistance. With 16 and 19 proteins or protein complexes involved in fluoroquinolone and glycopeptide resistances, respectively, and the complexities of bacterial sensing mechanisms that trigger and regulate a wide variety of possible resistance mechanisms, we propose that these antimicrobial resistance mechanisms might be considered complex ‘nanomachines’ that drive survival of bacterial cells in antibiotic environments.
Archaeological Heritage Management at the Memphis and its Necropolis World Heritage Site

Abstract This research explores archaeological heritage management at the Memphis and its Necropolis – the Pyramid Fields from Giza to Dahshur World Heritage Site in Egypt. The case study methodology involved a structured questionnaire in order to gather data from key stakeholders. The results suggest only partial implementation of the World Heritage Site (WHS) management plan due in part to financial constraints, while strategies to raise visitor numbers remain limited. Actions are required to promote tourism, and to find resources for site enhancements. Current site management responsibilities appear centralised, with a need to develop an integrated management plan for the WHS. There could be better understanding of the needs of visitors and how these can be met. The study recommends development of a more Integrated Management Plan for the site, perhaps also involving some restructuring of key (state) organisations and/or specifically the creation of an entity responsible for management of the site.
Austin Biomarkers & Diagnosis State Versus Trait Diagnostic Biomarkers in Psychiatry and the Issue of Translation

The trait versus state measures is highlighted as controversy in psychiatry and psychology. Trait markers are associated typically with retest stable genetic characteristics, such as personality dimensions. In clinical psychiatry trait biomarkers are incorporated into the endophenotype model. State dependent biomarkers on the other hand are case and state sensitive, appropriate for monitoring of disease course and outcome. There are reviewed Qualitative EEG (QEEG), structural and functional neuroimaging markers as related to different diagnosis and outcome from treatment in psychiatry. The issue of translation across disciplines involved in psychiatric diagnosis is considered to be underpinned by the concordance and synchronicity of the data under the model of trans-disciplinary validation.
The impact of early life antibiotic use on atopic and metabolic disorders: meta-analyses of recent insights Short title: Early antibiotic use and later-in-life health

The use of antibiotics in infancy has been suggested to increase the risks of atopic and metabolic disorders later in life. Through meta-analyses of more than 100 studies (>3.5mio children), we confirm these risks, and the observed patterns are consistent with this being due to microbiota-driven changes.
Stimulation of Dendritic Cells Pertussis Toxin Enhances Th1 Responses by

Pertussis toxin (PTX) has been widely used as an adjuvant to induce Th1-mediated organ-speciﬁc autoimmune diseases in animal models. However, the cellular and molecular mechanisms remain to be deﬁned. In this study, we showed that dendritic cells (DC) stimulated with PTX (PTX-DC) were able to substitute for PTX to promote experimental autoimmune uveitis (EAU). EAU induced by PTX-DC revealed a typical Th1 response, characterized by high uveitogenic retinal Ag interphotoreceptor retinoid-binding protein (IRBP)-speciﬁc IFN- (cid:1) and IL-12 production in the draining lymph nodes, as well as increased levels of anti-IRBP IgG2a and decreased levels of anti-IRBP IgG1 in the serum of IRBP-immunized mice. Furthermore, PTX-DC preferentially induced T cells to produce the Th1 cytokine, IFN- (cid:1) . After being stimulated with PTX, DC exhibited up-regulation of MHC class II, CD80, CD86, CD40, and DEC205. PTX-DC had also increased allostimulatory capacity and IL-12 and TNF- (cid:2) production. Serum IL-12 was increased in naive mice that received PTX-DC i.p. In addition, PTX activated extracellular signal-regulated kinase in DC. Following the inhibition of extracellular signal-regulated kinase signaling, the maturation of PTX-DC was reduced. Subsequently, the ability of PTX-DC to promote IFN- (cid:1) production by T cells in vitro and to induce EAU in vivo was blocked. The results suggest that PTX might exert an adjuvant effect on DC to promote their maturation and the production of proinﬂammatory cytokines,
Dimethylsulphide (DMS) emissions from the West Paciﬁc Ocean: a potential marine source for the stratospheric sulphur layer

Sea surface and atmospheric measurements of dimethylsulphide (DMS) were performed during the TransBrom cruise in the West Paciﬁc Ocean between Japan and Australia in October 2009. Air-sea DMS ﬂuxes were computed between 0 and 30 µmol m − 2 d − 1 , which are in agreement with those computed by the current climatol- ogy, and peak emissions of marine DMS into the atmosphere were found during the occurrence of tropical storm systems. Atmospheric variability in DMS, however, did not follow that of the computed ﬂuxes and was more related to atmospheric transport processes. The computed emissions were used as input ﬁelds for the Langrangian dispersion model FLEXPART, which was set up with actual meteorological ﬁelds from 10 ERA-interim data and di ﬀ erent chemical lifetimes of DMS. A comparison with aircraft in-situ data from the adjacent HIPPO2 campaign revealed an overall good agreement between modeled versus observed DMS proﬁles over the tropical West Paciﬁc ocean. Based on observed DMS emissions and the meteorological ﬁelds over the cruise track region, the model projected that up to 30 g S per month in the form of DMS can be 15 transported above 17 km in this region. This surprisingly large DMS entrainment into the stratosphere is disproportionate to the regional extent of the cruise track area and mainly due to the high convective activity in this region as simulated by the transport model. Thus, we conclude that the considerably larger area of the tropical West Paciﬁc
IoT Data Anonymisation for Building Energy Analysis

The use of IoT technology can help reduce the energy use of the built environment and increase occupant’s well-being, but growing privacy concerns may slow down its adoption. This project introduces an algorithm called Compact Auto-Encoder (CAE) that anonymises time-series data by taking into account data minimisation and self determinations principles outlined by the General Data Protection Regulation (GDPR), data utility loss and privacy gains. The CAE algorithm is composed of two neural networks, (1) an encoder responsible for creating a latent vector (z) and (2) a decoder that recovers relevant time-series information. During the prediction stage, edge devices host the encoder from the CAE algorithm, i.e. close to where data is being generated, whereas the data-recipient will host its corresponding decoder. Two training scenarios of the CAE algorithm are presented: unsupervised and supervised. The algorithm achieves 28% fewer utility losses in terms of Root Mean Squared Error (RMSE) and higher detection losses when transforming electricity data in the unsupervised scenario. On the second case, supervised anonymisation of CO2 data achieved 43% fewer utility losses and a 5% drop in its correlation with motion detection measurements, a sensitive measurement in the context of this work. The presented solution is considered an enabler of technologies that aim energy savings in the built environment by protecting user’s privacy when sharing the data that is needed to make more accurate analyses.
Dialysis Transplantation Incubation of porcine high-density lipoprotein with the apical surface of LLC-PK 1 renal tubular cells sustains the properties of orientated monolayers

Background. HDL is present in the urine of patients with the nephrotic syndrome. The amount of HDL is directly related to the protein selectivity and therefore to the renal prognosis. Urinary HDL may therefore be involved in the pathogenesis of progressive renal impairment in proteinuric renal disease. Methods. LLC-PK1 cells were grown as orientated monolayers on niters. Uptake of HDL and permeability to inulin were measured. The influence of HDL in the growth medium on monolayer resistance, protein content, and sodium dependent glucose transport was studied. The effects of tetranitromethane (TNM) nitrosylation of HDL and of albumin, mevalonate, or simvastatin were investigated. Results. Confluent LLC-PK1 monolayers took up fluorescently labelled HDL from either epithelial surface and formed a significant diffusion barrier to inulin. Monolayers incubated in 300 ug/ml HDL achieved a protein content and plateau of resistance equal to those in 10% fetal calf serum (FCS); 30-1000 ug/ml HDL applied to the apical surface of confluent monolayers maintained a plateau of resistance as well as 10% FCS and significantly better than serum-free medium. Sodium-dependent glucose transport was preserved in monolayers exposed to HDL. Simvastatin completely, and nitrosylation partially, removed the stimulatory properties of HDL. These were partly reproduced by albumin or mevalonate. Conclusions. HDL can enter renal epithelial cells from the apical surface. HDL added to this surface at confluence, reproducing the conditions found in the nephrotic syndrome, had a measurable positive effect on monolayer resistance. Results with nitrosylated HDL and HMG-CoA blockade suggest that these effects may be mediated via receptors and this enzyme system. Correspondence and offprint requests to: Dr C. P. Streather, Dept. of Medicine, King's College Hospital School of Medicine and Dentistry, Bessemer Road, London SE5 9PJ, UK.