Miracle-dz/UniFAR-FTUs huggingface dataset:
RAB GTPASE REGULATION OF APICAL CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR RECYCLING IN POLARIZED INTESTINAL EPITHELIAL CELLS

The regulated recycling of endocytosed membrane proteins at the plasma membrane is an important intracellular trafficking process that can regulate the copy number of proteins at the membrane and thereby modulate their function and the physiology of the tissue as a whole. The Cystic Fibrosis Transmembrane conductance Regulator (CFTR), a cAMP/PKA-activated anion channel, undergoes rapid and efficient recycling at the apical plasma membrane in polarized epithelial cells. The cellular mechanisms that facilitate CFTR recycling are understood poorly, especially in polarized cell systems, yet this process ensures the proper channel copy number at the apical membrane, and it is defective in the common CFTR mutant, ΔF508. Using a physiologically relevant model that recapitulates the in vivo secretory functions of the intestinal epithelia, I tested the hypothesis that distinct members of the Rab family of GTPases mediate the recycling of CFTR at the apical plasma membrane and that this process facilitates transepithelial anion secretion by maintaining a physiologically functional surface density of CFTR channels. The role of the Rab11 isoforms in CFTR recycling was investigated using the colonic epithelial cell line, T84, and rat intestine for in vivo studies. Immuno-isolation of Rab11 vesicles revealed endogenous CFTR within both the Rab11a and Rab11b compartments; however, only perturbing Rab11b expression or function attenuated the CFTR-mediated, cAMP-activated anion efflux. In polarized epithelial monolayers, mutant Rab11b inhibited the forskolin-stimulated transepithelial anion secretion by reducing the apical membrane density of CFTR. Biotin protection assays revealed a dependence of CFTR recycling on functional Rab11b, not Rab11a, demonstrating the selective requirement for the Rab11b isoform. Therefore, apical CFTR copy number is regulated by Rab11b-mediated recycling, which facilitates the agonist-stimulated, transepithelial anion response in polarized intestinal epithelial cells. The identification of Rab11b as a mediator of recycling reveals an apical recycling itinerary unidentified previously and emphasizes the importance of elucidating the membrane recycling itineraries in polarized epithelial cell models, which mimic the normal physiology of tissue. Understanding these processes within relevant models have direct implications for understanding normal cell biology and tissue physiology and form a foundation for future clinical therapies designed to correct disease processes arising from improper membrane recycling.
Accurate Personal Authentication by Combining Left and Right Palm Print Images

This paper develops the accurate personal identification by combining the left and right palm print Images. Recognition of persons by means of biometric characteristics is an important technology in the society, because biometric identifiers can’t be shared and they intrinsically represent the individual’s bodily identity. But in single biometric technology there is more number of chances for fraudulent activities. When we use the multi biometric, it can provide higher accuracy than any other technologies. Among those palm print identification has received much attention because of its good performance. Combining the left and right palm print images to perform multi biometric is easy to implement and can obtain better results. In this paper, we proposed a novel framework to perform multi biometrics by comprehensively combining the left and right palm print images. This framework integrated three kinds of scores generated from the left and right palm print images to perform matching score-level fusion. The first two kinds of scores were, respectively, generated from the left and right palm print images and can be obtained by any palm print identification method, whereas the third kind of score was obtained using a specialized algorithm proposed in this paper.
Reinforced logistics: Automated tour planning with reinforcement learning

In times of ever-growing and flexible demand for freight transports, the ability to quickly create efficient tour plans becomes increasingly important. Currently, this process is often carried out manually and requires trained personnel with years of experience. Due to the increasing number of goods to be shipped, as well as short reaction and planning times, this task becomes more and more complex and time-consuming. In this paper, we present a novel approach for automated tour planning based on reinforcement learning methods. This enables us to learn on the basis of historical tour data, react flexibly to changes in transport conditions, and create tours similar to the ones created by human planners. Our focus is on short-range business-to-business transports, where the delivery areas are not as densely populated with customers as within the consumer transport sector.
Production and economics of edible oyster culturedin an estuarine system of Kerala

Edible oyster Crassostrea madrasensis locally known as "Kadal muringa" In Malayalam occurs In the intertidal zones and estuaries. They are exploited by flshermen and sold in the local market. Oyster culture Is practised mainly in temperate countries and the annual world production of oyster by aquaculture was 9,52,195 tonnes in 1992. Many countries in the tropics have embarked upon programmes to develop oyster culture in view of the growing demand for oyster meat in the international market.
A simple modified method of DNA extraction from seeds for PCR amplifications

Rapid and reliable protocols for testing genetic purity of seed are an important requirement for quality assurance. This method was applied to Indian mustard, rice, wheat and cotton. Yield and quality of DNA was good for PCR analysis. DNA yield ranged from 16.4 to 76.22 ng/mg seed depending on plant species and varieties. The A260/280 ratio ranged from 1.65-1.91, indicating that the isolated DNA was fairly clean and free from protein and RNA contaminations. By adopting this method nearly 200–300 sample can be extracted in 24 hr, which will be useful for assessment of genetic purity of seed lots during seed multiplication as well as in breeding programmes that requires rapid screening of large population.
Enabling white-box reasonings on black-box composition operators in a domain-independent way. (Un modèle de raisonnement pour les opérateurs de composition logicielle décrits en boite noire)

The complexity of software systems made it necessary to split them up and reunite them afterward. Separating concerns is a well-studied challenge and teams separate the work to be done beforehand. Still, separating without considering the recomposition leads to rushed, unsafe, and time-consuming recomposition. The composition should create the right and best system with minimal human effort. Composition operators are often ad-hoc solutions developed by non-specialist development teams. They are not developed using high-level formalism and end up being too complicated or too poorly formalized to support proper reasonings. We call them "black-boxes" as existing techniques requiring knowledge of its internals cannot be applied or reused. However, black-box operators, like others, must ensure guarantees: one must assess their idempotency to use them in a distributed context; provide an average execution time to assess usage in a reactive system; check conflicts to validate that the composed artifact conforms to business properties. Despite the black-box aspect, none of these properties are domain-specific. In this thesis, we present a domain-independent approach that enables (i) reasonings on composition equation, (ii) to compose them safely, (iii) by assessing properties similar to the ones from the state-of-the-art. We validated the approach in heterogeneous application domains: 19 versions of Linux kernel with 54 rewriting rules, fixing 13 antipatterns in 22 Android apps, and validating the efficiency of the approach on the composition of 20k Docker images.
Mission Profile Based Reliability Evaluation of Building Blocks for Modular Power Converters

The major challenge of the power electronics design is to be cost effective while fulﬁlling the reliability requirements. Since the thermal cycling is one of the most severe causes of failure in power electronics, this paper presents a test bench for the thermal testing of power converters. This is particularly important for modular power converters consisting of several building blocks, which cannot be tested easily. For thermal design evaluation it is proposed to test the building blocks under real mission proﬁles. As a study case, the thermal behavior of an Modular Multilevel Converter (MMC) is given to demonstrate the approach.
Outdoor People Tracking

We address the problem of automatically detecting and tracking outdoor people. We propose to combine background subtraction based tracking with tracking-by-detection approach in a Kalman filtering framework. For the detection purpose, we use HOG detector, which detects people only in those regions suggested by background subtraction model. Since detectors may give false positives or miss targets, background subtraction is used to complement the detector outcomes.
Web-Based Program Exposure and Retention in the Families Improving Together for Weight Loss Trial.

BACKGROUND Interventions that incorporate behavioral skills training and parental involvement have been effective for promoting weight loss among middle and upper class youth; however, few studies have produced similar weight loss effects in underserved ethnic minority youth. PURPOSE This study examined whether online program exposure (in both an online tailored intervention and an online health education comparison program) predicted greater retention among African American youth and their parents in the Families Improving Together (FIT) for Weight Loss trial. METHODS Parent-adolescent dyads (N = 125) were randomized to either an online tailored intervention program (n = 63) or an online health education comparison program (n = 62). Paradata including login data were used to determine the number of sessions viewed (0-8) and the number of minutes spent online per session. Study retention, defined as collection of adolescent anthropometric measures at 6 months postintervention, was the outcome. RESULTS Logistic regression analyses showed a significant effect for login rate on retention (OR = 1.21, 95% CI [1.04, 1.39]). Total number of sessions viewed, child age, child sex, parent age, and parent sex accounted for 11% of the variance in retention at 6 months post- intervention. Participants who were retained spent a significantly greater number of minutes during each session (M = 12.99, SD = 11.63) than participants who were not retained (M = 7.77, SD = 11.19), t(123) = 2.24, p = . 027, d = 0.45. CONCLUSIONS The use of paradata from online interventions is a novel and feasible approach for examining exposure in web-based interventions and program retention in underserved ethnic minority families. TRIAL REGISTRATION ClinicalTrials.gov NCT01796067. Registered January 23, 2013.
The plastic self : investigating the processes of self-face recognition

This thesis investigates self-face recognition and the plasticity of self-representations through several different behavioural and brain stimulation manipulations. The experiments in Chapters 3 and 4 explore the involvement of the temporoparietal area in self-other discrimination processes. Using transcranial direct current stimulation (tDCS) to modulate neural excitability in this region, the experiments in these chapters explore self-other face discrimination and mental body rotation. Enhancing neural excitability in this area appears to enhance familiar other-recognition and the ability to take another’s spatial perspective. Chapters 5 and 6 target brain areas associated with multisensory bodily illusions with tDCS, and investigate their involvement in self-updating. Chapter 5 explores the contribution of the intraparietal sulcus to multisensory integration during the Enfacement illusion. Chapter 6 investigates the effect of tDCS on somatosensory cortex during a modified enfacement illusion paradigm to investigate self-updating. Chapters 7 and 8 explore the novel use of a self-association paradigm to update bodily and conceptual aspects of self. The experiments in these chapters demonstrate that a previously unfamiliar face can be incorporated into the conceptual self-representation. Chapter 7 investigates the existence of a bidirectional modulatory link between conceptual and bodily aspects of self, and found that changes to the conceptual self did not have an effect on the bodily self-representation. The experiment in Chapter 8 investigates the nature of the association created between the self-representation and the novel face in Chapter 7 by testing whether the newly associated face affects recognition of well-established perceptual and conceptual aspects of self.
Collaborating to improve Healthcare: A case study

To tackle the societal challenges of the blooming Healthcare sector, different organizations are increasingly seeking to connect and cooperate. They are looking towards a more holistic vision of Healthcare in which products or services are not simply aiming to suppress a particular illness, but at increasing well-being in patients. It is deemed of primary importance to include patients in innovation processes, as innovators must be able to grasp the complexity of their needs if they want to meet them accordingly. That is what the Crowd Ideas project aims to achieve when it comes to breast cancer care in the Västra Götalands region. The pilot project assembles actors from the private, public, adademic and civil sectors, around the goal of improving the life of people affected by breast cancer. This report examines the way these actors with different organizational cultures and goals managed to work together and come up with innovative solutions. To investigate this cross-sector collaboration, the author conducted semi-open interviews of the members of the steering committee of Crowd Ideas as well as sent them a survey. The study revealed that high levels of jointness in decision-making processes, as well as the building of administrative and social leadership mechanisms, were important for this collaboration to take decisions and action. It proved equally important to establish mutual partner relationships in which organizations are open about sharing their interests and resources. Furthermore, a trust building process between actors enabled the innovative project to be pursued without formal hurdles. However, the author has identified a need for new legal tools to facilitate cross-sector innovative collaborations, especially concerning the joint ownership of project results, which is a sensitive issue. More research has to be conducted in this area to encourage the Healthcare ecosystems to co-innovate with increased oppenness. TABLE OF CONTENTS CHAPTER
Exploring the boundaries of shallow phylogeny in the YESS group and the dynamics of gene cluster and operon formation in bacterial genomes

In this thesis I look at two different problems in bacterial genomic analysis. The first involves reconstructing the evolutionary history between a group of closely related bacteria. I addressed whether or not it is possible to separate such genomes into different genera, species and strains. Specifically, I addressed how different approaches such as the use of 16S rRNA phylogenetic trees, phylogenetic supertrees and concatenation of individual genes in order to construct phylogenetic trees compare with one another. What effect will problems associated with resolving shallow-phylogeny have on recovering a tree of life? Ultimately I show that for the group of genomes involved, different methods and data produce different results and that the true tree, if a tree-like structure does indeed exist for these genomes, is unrecoverable using such approaches. In the second part of my thesis I examine the phenomenon of gene clustering in bacterial genomes. I present a software program, GenClust, for the identification, analysis and visualisation of gene clusters. I show how GenClust can be used to recover and analyse clusters of genes involved in amino acid biosynthesis across a large ! -proteobacterial dataset. Finally, I examine models of gene cluster and operon formation and test them with real data, using a combined approach of comparing clusters on both structural similarity and the underlying phylogenetic signals of the clustered genes. I provide a hypothesis for the selective forces driving cluster and operon formation in bacterial genomes.
Improving WiMAX Capacity Of A Cell By Applying Relay Stations And Adaptive Antenna Systems

Worldwide interoperability for Microwave Access (WiMAX) is a broadband wireless technology based on IEEE 802.16 standard . A WiMAX cell which is to be considered in this study applies multicast mode of operation , the main limitation of maximizing the capacity of the system is the signal to noise ratio (subscribers near the base station apply 64 QAM and 3⁄4 Reed Solomon code , while those which are near the border of the cell applies PSK and 1⁄2 Reed Solomon code to compensate for the S/N effect). Results indicate that better capacity is achieved by introducing Relay Stations (RSs) and Adaptive Antenna System (AAS) to the cell with TDD mode of operation, other factors like MAC and PHY layers overheads are studied and taken into account in the calculation of the system capacity .
Un nuevo mtodo de anlisis digital para la determinacin de los niveles de consumo foliar

A protocol using the computer program Adobe Photoshop CS2 (version 9.0) is proposed for determining the level of herbivory on intact and consumed leaves, using mangroves as an example. The usual manual methods consist of drawing the leaf on graph paper, restoring the original shape in cases of damaged edges. The computerized methods are intended to accelerate the leaf measurement time with applications, digitalization equipment, and portable area measurers that are more efficient, faster, and more economically viable than the manual methods. The need to restore the edges of the consumed leaves is an obstacle to all methodologies. Nonetheless, the reconstruction of the consumed foliar edges is shown to be more realistic with Adobe Photoshop, as this allows a better fit to the original shape of the leaf. Moreover, this application allows the use of compacted files such as JPEG, permitting a simple, rapid, and precise method for determining the levels or rates of foliar herbivory.
Challenges of Men in Early Childhood Education -- Case Study of an American Male Early Childhood Teacher

Early childhood education is recognized as a gender-atypical occupation for men, so men who choose to teach at this level are often under public suspicion and scrutiny. This paper focuses on the experiences of an American male early childhood teacher for an in-depth understanding of the nature of his experiences. Semi-structured interviews were conducted with the participant to elicit his perspectives. Moreover, data of observation and document review were also provided to guarantee the credibility of the findings. It was found that the participant was confronted with many challenges in his interaction with female colleagues, parents and students. Further, these challenges were deeply rooted in traditional gender norms of the culture. Keywords—early childhood education; gender; masculinity; challenge
Effect of treatment with carnosin on cataract development in experimental ocular hypertension

E-mail: iradoc@mail.ru Background: Senile cataract and glaucoma are the major age-related and degenerative ocular disorders that can cause loss of vision and blindness. To date, the question of the possible mechanisms involved in the impact of the glaucomatous process on the lens is still open. Purpose: To investigate the effect and mechanism of action of carnosine on the light-induced cataract model in the presence of experimental ocular hypertension (OHP). Materials and Methods: Rabbits were exposed to light from mercury arc-discharge lamps (type DRF-1000, 1000 W; bandpass range, 350-1150 nm) for 9 hours a day during a 10-week period to investigate the effect of regularly instilled (twice daily during the same period) carnosine on the development of opacities and thiol levels in the lens in the light-induced rabbit cataract model. OHP was induced bilaterally by injecting 0.1 mL of 0.3% carbomer solution into the anterior chamber of both eyes. Results: Mild changes in lens clarity (grades 1 to 3) were more common in rabbits treated with carnosine, whereas more apparent changes in lens clarity were more common in untreated animals. At 10 weeks, grade 5 lens opacity was noted in 8.3% of the rabbits treated with carnosine and in 20.3% of the untreated animals. Introduction of carnosine in the rabbit model of light-induced cataract in the presence of OHP resulted in 49.2% increase in the reduced glutathione levels, 28.3% decrease in oxidized glutathione levels, 40.7% increase in the sulfhydryl (thiol) levels and 28% decrease in the disulphide levels in the lenses of treated experimental animals compared to untreated experimental animals. Conclusion: A long-term intraocular treatment with carnosine in the rabbit cataract model (a) improved the resistance of the lens to cataractogenic phototoxicity in the presence of ocular hypertension and (b) contributed to substantial normalization of thiol levels in the lens, thus preventing oxidative damage to proteins.
Faithful visualisation of similarities in high dimensional data

In the last fifteen years, new methods of dimension reduction have been invented that enable much improved visualisation of high-dimensional data-sets. Conventionally, the data-sets are visualised as two-dimensional scatterplots, and similarity relationships between data-cases are revealed by grouping and proximity of points in the plane. But the arrangement of points in a 2D scatterplot cannot faithfully represent complex high-dimensional structure: more expressive 2D visualisations are needed. This thesis develops new types of diagram that can represent data-similarities more expressively than a mere scatterplot. The approach is to automatically select a graph to overlay on the scatterplot, in order to enable a richer visualisation of similarities than is possible by the arrangement of points alone, and to correct distortions inherent in scatterplot visualisation. Methods and software are developed for selecting and graphically representing the overlay graph as a diagram that humans can read. These diagrams enable correct and informative human interpretation of scatterplots that would otherwise be hard to interpret or misleading.
A Review on different heirarical routing protocols for WSN using Moving Base Station.

: WSN is the wireless sensor network have various network related issues used as wireless network for small difficult terrain. Each node in the sensor network is battery operated. The life time of the node is directly is dependent on the direct battery usage. While transmission of the sensed data and while receiving the data large amount of energy will be required. Various protocols based on different type of working techniques are being researched to minimizes the energy usage. Less energy means more life time of the node. The hierarchical based routing protocols are most suitable type for energy saving. Because it sub divide the total network into smaller clusters. Each cluster will be having randomly distributed nodes. Based on residual energy a cluster head will be selected. Each sensor node has to sends the data to the cluster head. From cluster head to second level cluster head then to the base station. The chain or hierarchy of the network depends upon the size of the network area. There are various research papers which has mentioned the network with moving base station. Base station is energy harvested. It can move on fixed type of path. While moving on the path it collects the data from the cluster head. This will in result in less wastage of the energy.