Miracle-dz/UniFAR-FTUs huggingface dataset:
Activation and Cytokine Response Vivo Augmentation of T and B Cell TIRC7 Deficiency Causes In Vitro and In

The membrane protein T cell immune response cDNA 7 (TIRC7) was recently identiﬁed and was shown to play an important role in T cell activation. To characterize the function of TIRC7 in more detail, we generated TIRC7-deﬁcient mice by gene targeting. We observed disturbed T and B cell function both in vitro and in vivo in TIRC7 (cid:1) / (cid:1) mice. Histologically, primary and secondary lymphoid organs showed a mixture of hypo-, hyper-, and dysplastic changes of multiple lymphohemopoietic compartments. T cells from TIRC7 (cid:1) / (cid:1) mice exhibited signiﬁcantly increased proliferation and expression of IL-2, IFN- (cid:2) , and IL-4 in response to different stimuli. Resting T cells from TIRC7 (cid:1) / (cid:1) mice exhibited decreased CD62L, but increased CD11a and CD44 expression, suggesting an in vivo expansion of memory/effector T cells. Remarkably, activated T cells from TIRC7 (cid:1) / (cid:1) mice expressed lower levels of CTLA-4 in comparison with wild-type cells. B cells from TIRC7-deﬁcient mice exhibited signiﬁcantly higher in vitro proliferation following stimulation with anti-CD40 Ab or LPS plus IL-4. B cell hyperreactivity was reﬂected in vivo by elevated serum levels of various Ig classes and higher CD86 expression on B cells. Furthermore, TIRC7 deﬁciency resulted in an augmented delayed-type hypersensitivity response that was also reﬂected in increased mononuclear inﬁltration in the skin obtained from TIRC7-deﬁcient mice food pads. In summary, L H inducible NO synthase indirect immunoperoxidase polyclonal L H NO
Social Entrepreneurs’ Musings: A Premise for Value Creation

The purpose of this study is to identify the gap left behind by conventional medical practices which is filled by non-allopathic medicine and the motivation behind initiation of this step by founders/creators of such businesses. Semi structured interviews are conducted from non-allopathic service provider organizations and interview data is analyzed to detect themes behind their motivations and ultimate ob-jective of conducting such a business in a non-favorable market. Identification of issues is synonymous with founder’s prior experience in said context, market opportunity identification, financial survival and suggestion of a viable solution to address the issue on sustaina-ble grounds is the objective. The classification of social enterprise businesses is dependent on creation of social value primarily and financial value on secondary level through provision of alternative solutions. This study takes a new step in identifying market gaps on the basis of interchangeable financial to social value objectives.
A SEMIPARAMETRIC REGRESSION MODEL FOR PAIRED LONGITUDINAL OUTCOMES WITH APPLICATION IN CHILDHOOD BLOOD PRESSURE DEVELOPMENT 1

This research examines the simultaneous inﬂuences of height and weight on longitudinally measured systolic and diastolic blood pressure in children. Previous studies have shown that both height and weight are positively associated with blood pressure. In children, however, the concurrent increases of height and weight have made it all but impossible to discern the effect of height from that of weight. To better understand these inﬂuences, we propose to examine the joint effect of height and weight on blood pressure. Bivariate thin plate spline surfaces are used to accommodate the potentially nonlinear effects as well as the interaction between height and weight. Moreover, we consider a joint model for paired blood pressure measures, that is, systolic and diastolic blood pressure, to account for the underlying correlation between the two measures within the same individual. The bivariate spline surfaces are allowed to vary across different groups of interest. We have developed related model ﬁtting and inference procedures. The proposed method is used to analyze data from a real clinical investigation.
Structural Analogues in Human Colon Carcinoma HT-29 Cells in Vitro1

The new Adriamycin (ADR) analogue, 3'-(3-cyano-4-morpholinyl)-3'-deaminoadriamycin (CMA), is the most potent anthracycline yet devel oped. The cellular pharmacology of CMA and . V-(4-morphulmyl)-. V-deaminoadriamycin (MA), and their 5-imino derivatives, ICMA and IMA, were compared with ADR in a human colon carcinoma (HT-29) cell line in vitro. In a soft agar clonogenic assay, the order of antitumor activity was CMA>ICMA>ADR>MA>IMA, for both 2- and 24-h drug exposure periods, indicating a requirement for the cyanide group and an intact quinone ring for the potent antitumor effect of CMA. The cellular uptake of CMA was 2-fold less than that of MA, although, consistent with its greater nuclear binding, the degree of efflux of CMA was less than that of MA. The order of cytotoxicity of the analogues correlated approximately with their effects on cellular DNA synthesis, indicating that this feature may contribute to the antitumor effect. Using isolated nuclei, the order of inhibition of DNA transcription by the analogues was ( MA>M Y>AI >R, which was similar to their nuclear affinities, suggest ing that their effects on cellular nucleic acid synthesis were due to a direct interaction of drug with DNA. However, CMA did not appear to differ from the other drugs in its base specificity as all the analogues preferentially inhibited Escherichia coli RNA polymerase activity di rected by poly(dAdT) â€¢poly(dAdT) compared to poly(dGdC) â€¢poly(dGdC).
cd T cells promote the maturation of dendritic cells during West Nile virus infection

gd T cells are important for the early control of West Nile virus (WNV) dissemination. Here, we investigated the role of gd T cells in the regulation of CD4 1 T-cell response following a WNV challenge. Splenic dendritic cells (DCs) of WNV-infected gd T-cell-deﬁcient (TCR d (cid:2) / (cid:2) ) mice displayed lower levels of CD40, CD80, CD86 and major histocompatibility complex (MHC) class II expression and interleukin-12 (IL-12) production than those of wild-type mice. Naı¨ve DCs cocultured with WNV-infected gd T cells showed enhanced levels of costimulatory molecules, MHC class II expression and IL-12 production. Further, coculture of CD4 1 T cells from OT II transgenic mice with DCs of WNV-infected TCR d (cid:2) / (cid:2) mice induced less interferon- g (IFN- g ) and IL-2 production than with those of wild-type controls. Viral antigens were detected in WNV-infected gd T cells.WNV infection or toll-like receptor (TLR) agonist treatment of gd T cells induced the production of IFN- g , tumor necrosis factor- a and IL-6, which are known to promote DC maturation. Nevertheless, the levels of TLRs 2, 3, 4 and 7 expression of WNV-infected gd T cells were not different from those of noninfected cells. Overall, these data suggest that WNV-induced gd T-cell activation promotes DC maturation and initiates CD4 1 T-cell priming.
Facial Recognition and Emotion Detection System for Dynamic Advertisement Allocation

—Advertisements represent a persuasive method of communication for convincing people to change their thoughts or attitudes. Conventional advertisements do not always provide optimal marketing effectiveness because the advertisements are presented uniformly to viewers. To overcome the limitations of traditional methods in advertising research, a dynamic advertisement model is proposed in this paper, and facial expression detection is applied to real-time measurement during media exposure. This is a novel model to recognize viewers’ facial expression for emotion regulation and then adjust the decision of content sequence according to their emotions. A decision tree algorithm is used, and each demographic measurement results from a few scenarios. The decision is determined through bottom-up branch searching. Based on the study results, personalized advertising and audience targeting with accurate facial expression analysis can allow marketing and advertising researchers to better understand viewers’ emotional valence and behavior and to employ mathematical formulation for establishing the optimal advertising approach.
Refusal Parenting Style is Easier to Perceive Non-physical Bullying for Students in the Upper Grades of Elementary School: Mediation Effect of Negative Affect Experience and Negative Coping Style

: To explore the impact of parenting styles on the perception of campus non-physical bullying, 492 students in upper elementary school grades were surveyed by using the Delaware Bullying Victimization Scale, the Negative Coping Style Scale, the Negative Affect Scale, and the Egna Minnen Beträffende Uppfostran Questionnaire. The questionnaire survey was conducted in the fifth and sixth grades of eight primary schools in Zhejiang province. The results showed that cyberbullying was not significantly related to an anxious parenting style, but negative affect experiences, negative coping styles, negative family parenting styles, and the perception of campus non-physical bullying were all positively correlated with each other ( p < 0.05). The refusal parenting style was shown to be an important factor that affected students’ perception of campus non-physical bullying; it was observed to directly affect students’ perception of campus non-physical bullying and indirectly affect students’ perception of campus non-physical bullying by influencing negative affect experiences and negative coping styles. In conclusion, negative affect experiences and negative coping styles had a chain-like mediating effect between the refusal parenting style and students’ perception of campus verbal bullying. Moreover, negative affect experiences had a partial mediating effect between the refusal parenting style and students’ perceptions of campus cyberbullying, relationship bullying, and non-physical bullying total scores. Implications and suggestions based on these results are also discussed.
A CTIN INTERACTING PROTEIN 1 CONTROLS ASSEMBLY AND PERMEABILITY OF INTESTINAL 1 EPITHELIAL APICAL JUNCTIONS 2

Adherens junctions (AJs) and tight junctions (TJs) are crucial regulators of the integrity and 36 restitution of the intestinal epithelial barrier. The structure and function of epithelial junctions 37 depends on their association with the cortical actin cytoskeleton that, in polarized epithelial cells, 38 is represented by a prominent perijunctional actomyosin belt. The assembly and stability of the 39 perijunctional cytoskeleton is controlled by constant turnover (disassembly and reassembly) of 40 actin filaments. Actin interacting protein (Aip) 1 is an emerging regulator of the actin 41 cytoskeleton, playing a critical role in filament disassembly. In this study, we examined the roles 42 of Aip1 in regulating the structure and remodeling of AJs and TJs in human intestinal epithelium. 43 Aip1 was enriched at apical junctions in polarized human intestinal epithelial cells and normal 44 mouse colonic mucosa. Knockdown of Aip1 by RNA interference increased the paracellular 45 permeability of epithelial cell monolayers, decreased recruitment of AJ/TJ proteins to steady- 46 state intercellular contacts, and attenuated junctional reassembly in a calcium switch model. The 47 observed defects of AJ/TJ structure and functions were accompanied by abnormal organization 48 and dynamics of the perijunctional F-actin cytoskeleton. Moreover, loss of Aip1 impaired the 49 apico-basal polarity of intestinal epithelial cell monolayers, and inhibited formation of polarized 50 epithelial cysts in 3-D Matrigel. Our findings demonstrate a previously unanticipated role of 51 Aip1 in regulating the structure and remodeling of intestinal epithelial junctions, and early steps 52 of epithelial morphogenesis. 53
Acute Toxicity of Water Soluble Fraction of Crude Oil to the Early Life Stages of the African Catfish ( Clarias gariepinus )

Acute toxicity bioassay of water soluble fraction of crude oil (Escravos blend), was conducted to evaluate the toxicity on the early life stages of the African catfish ( Clarias gariepinus ) under static bioassay conditions. Five concentrations of water soluble fraction (WSF) of the crude oil (0, 1.25, 2.5, 5, 10 and 20%) were constituted in glass aquaria (25 cm ´ 24 cm ´ 10 cm). Twenty laboratory-reared frys were introduced in triplicate in each of the test aquaria and exposure conducted for 96 h. The median lethal concentration (LC 50 ) and the 95% confidence interval was estimated using Trimmed Spearman-Karber method. The physicochemical characteristic of the test media was also evaluated. The 96 h median lethal concentration (LC 50 ) at 95% confidence interval was estimated to give 2.84% (upper and lower confidence interval of 3.83% and 2.10%) of the water soluble fraction. There was no marked change in pH of the test media compared to the control. However, the dissolved oxygen content and the conductivity showed a concentration dependent decline in the test media when compared to the control. The frys were observed to be weak and swimming activities were reduced. It can be concluded from the study that the water soluble fraction of crude oil (Escravos blend) is toxic to early life stages of C. gariepinus .
To study the knowledge about tuberculosis management and national tuberculosis program among medical students and aspiring doctors in a high tubercular endemic country

Background : More than eight million people develop active tuberculosis annually and most of the cases are detected in the developing world. This study aims to describe the knowledge about tuberculosis management and knowledge about national tuberculosis control program among medical students and young doctors in a developing country. Materials and Methods : A questionnaire based survey was conducted among 112 interns and post graduate students of a tertiary care hospital to study the knowledge about tuberculosis and national tuberculosis control program among these doctors. Results : Of the 112 respondents to the questionnaire The number of sputum specimens required for diagnosis under RNTCP was responded correctly by only 57%, the time duration in which sputum specimen should be processed was told correctly by only 42.5%, while the sputum examination guidelines for extra-pulmonary tuberculosis was responded correctly by only 27%. The awareness of serious forms of sputum-negative pulmonary tuberculosis was 21%, while serious form of extra pulmonary tuberculosis was known to be only 33%. The correct categorization of tuberculosis patients was done by only 56% of the respondents, while treatment of tubercular meningitis was marked correctly by 69% of the respondents. Conclusions : This study indicates a low level of knowledge among participants despite DOTS covering the entire country at present. This study indicates an enormous challenge and an urgent need to revamp and reform undergraduate medical education and change in medical curriculum with need instituting practical training at Directly Observed treatment Short Course (DOTS) centers in the curriculum of medical students in India.
Applicability of Okun’s Law in Nigeria: A Critical Evaluation: An Autoregressive Lag (ARDL) Model

The research focused on evaluating the relevance of Okun’s Law in Nigeria’s economy. Okun’s law suggest that a 3:1 negative relationship exists between output growth (GDP) and unemployment rate. The theory suggests that increases in GDP will automatically leads to a reduction in unemployment rate. We made use of recent data set between 1980 – 2017 obtained from the Central Bank of Nigeria’s (CBN) Statistical Bulletin and extracts from National Bureau of Statistics (NBS). Having ascertained that the data set were fractionally integrated of order-zero I(0) and order-one I(1), we applied the Autoregressive Distributed Lag Model. The Bounds Test conintegration result indicates that a longrun relationship exists between the variables. Bearing in mind the influence of oil in Nigeria’s economy, we introduced oil revenue as a check variable to align with realities. The result emanating from the estimated shortrun coefficients indicate that with passage of time, a positive and significant relationship emerge between output growth and unemployment rate in Nigeria. As Nigeria witnessed substantial increases in GDP, her unemployment rate was equally growing. Our finding is at variance with Okun’s law and by implication invalidates its application in Nigeria. The findings provided evidence that output growth in Nigeria is non-inclusive and such demonstrates the attributes of “Jobless” growth syndrome. Based on the findings, we suggest that policy makers should shift attention from GDP growth as such could be misleading; rather they should pay attention to structural transformations that will enhance job creation and reduction of poverty. means that the Okun’s law does not apply in Nigeria rather it could be country specific. Our findings show that contemporaneously, there exists a negative relationship of -0.218 between unemployment rate and economic growth but the relationship is not significant and also it is less-than the Okun’s coefficient of 3:1 relationship. It further reveals that with passage of time, a positive rather-than negative relationship exists between the two variables. This
Implementing Factors of Information Security in Governmental Organizations of Jordan

— To maintain the integrity of information technology in governmental organizations, efficacious success factors in information security must be applied. Upon broad assessment and screening on the factors that influence information security, no permutation to successful assessment was reached. In addition, the influence of such factors has yet to be evaluated across the stages of assessment. This research’s objective is the discovery of successful implementation of information security factors.
Convergence in Green Growth as the Key to Fighting Climate Change, 1990–2019

The consequences of climate change are an important point of contention in the current policy debate on the sustainability of economic development and growth. Climate change contributes to many socio-economic disadvantages and risks. The primary objective of global policy 2050 is to fully understand the negative externalities of environmental change. Goal and methodology: the main goal of the study is to examine convergence to the steady green growth state for a sample of 130 countries from 1990 to 2019. Setting up an efficient and consistent policy to fight climate change demands empirical knowledge on green growth. Using log (t) test methodology, we investigate convergence in green growth indicators for selected samples based on available country data. Findings: our assumptions are: (1) economies are not motivated and eager to foster energy transition; instead, they accept it as a random policy goal and (2) economies want to foster energy transition, but they are limited by a significant trade-off factor pulling their efforts down. Convergence testing shows the existence of two significant convergence clubs, one with countries moving to energy transition and the second still promoting national output based on fossil fuels. The findings indicate future divergence between the two clubs and significant convergence within identified convergence clubs. Conclusions: the energy transition is moving forward at two-tier speed with green leaders and green follower clubs. Policymakers and practitioners must closely monitor the dynamics of green growth to assess the risk and uncertainties of climate change.
ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF SOME TERPENOIDS AND FLAVONOIDS FROM ROOTS OF BERGENIA LIGULATA (SAXIFRAGACEAE)

: For the first time five compounds have been isolated from the roots of Bergenia ligulate . Lanost-7(8)-en-3  -formyl-6  -acetoxy-11  ,15  -diol-26-heptanoxy-27-oic acid, Urs-12(13)-en-3  -22  -diol-15  -formyl-21  glucoside, 9,11-Seco-lanost-20(22)-en-3  -formyl-18-phenoxyoate-12  -D-glucose, Sumatrol, and 5`-Methyleriodictyol-3`-O-  -D-galactopyranosyl(1  4)-  -L-rhamno-pyranose. Their structures were elucidated by chemical and physical data (IR, UV, 1 H-NMR, 13 C-NMR and Mass spectra).
EFFECT OF MONENSIN AND PROPYLENE GLYCOL AS A PROPHYLACTIC TOOLS FROM CAPRINE LAMB LOSSES AND THERE EFFECTS ON CONCEPTION RATES

Thirty does at the late stage of pregnancy were used in this study, aged 3-5 years and housed under the same environmental conditions in the same locality (Cairo-alexandria desert area). All does were given antiparasitic treatment for external and internal parasites. The does were divided into 3 groups, group1 (N=10) used as a control group, group 2 (N=10) supplemented with monensin extract as a premix in a dose of 2.72 mg/head/day for 15 successive days. Group 3 (N=10) were drenched with propylene glycol in a dose of 100 ml/head/day for 15 successive days. The treatment was done at the beginning of 4 th month of pregnancy. Serum samples were analysed from all treated and control groups for blood level of glucose, urea, calcium, triglyceride, total cholesterol, inorganic phosphorous, BHB, NEFA, insulin, liver enzymes and thyroid hormones. Present results revealed that elevation of serum calcium, cholesterol, insulin, glucose, BHB and NEFA in propylene treated group while liver enzymes, total triglycerides and thyroid hormones was decreased followed by monensin treated group in the same manner. The study also illustrated shortened postpartum open days in propylene treated group followed by monensin treated group. while conception rates was significantly increased in propylene drenched group. The study preferred uses of propylene glycol and monensin extract for pregnant does at the late stage of pregnancy for prophylaxis of lamb losses and also for enhancing some fertility parameters.
HIV_A_301510 581..592

1Benishangul-Gumuz, Wombera Sineor Secondary and Preparatory School, Benishangul, Ethiopia; 2University of Gondar, College of Natural and Computational Science, Departmentof Statistics, Gondar, Ethiopia Background: Among the many worldwide health problems, HIV/AIDS has caused severe health problems in several countries. The problem is also widely seen in Ethiopia. The general objective of the study is to cluster HIV patients and to find out the factors that mostly affect the prevalence of HIV within a group (cluster) and between groups (clusters) of HIV patients. Methods: The study is made based on the 2016 Ethiopian Demographic Health Survey (EDHS) which was collected by the Central Statistical Agency (CSA) of Ethiopia, and the survey collected a total of 26,753 samples, of which 14,785 were women and 11,968 were men and the age group was between 15 and 49 years for both. Binary logistic regression, principal component analysis, cluster analysis, and ANOVA were applied to analyze the data. Results: The result from binary logistic regression reveals that 15 factors such as ever heard of AIDS, region, water not available for at least a day in the last 2 weeks, has a radio, family members wash their hands, location of the source of water, everything completed to water to make it harmless to drink, food cooked in the house/separate house/outside, has a mobile telephone, has a table, type of place of residence, highest education level attained, current marital status, sex of household members, and age of household members are all significant factors that affect HIV status. Conclusion: Using these significant variables, 12 principal components are identified which describe 78% of the variation in the data. The result of HIV patients are clustered into 3 clusters and determine the status of HIV levels. Mainly, cluster 2 accounts for 50% of HIV patients whereas cluster 3 and 1 accounts for 40% and 10%, respectively.