Miracle-dz/UniFAR-FTUs huggingface dataset:
Associations between results of post-stroke NDT-Bobath rehabilitation in gait parameters, ADL and hand functions.

BACKGROUND In patients after a stroke there are variable disorders. These patients often need rehabilitation in more than one area beceause of multiple limitations of the ability to perform everyday activities. OBJECTIVES The aim of the study was to assess correlations - statistical relationships between observed gait parameters, ADL and hand functions - results of rehabilitation of patients after ischaemic stroke according to the NDTBobath method for adults. MATERIAL AND METHODS The investigated group consisted of 60 patients after ischaemic stroke, who participated in the rehabilitation programme. 10 sessions of the NDT-Bobath therapy were provided in 2 weeks (10 days of the therapy). The calculation of correlations was made based on changes of parameters: Bobath Scale (to assess hand functions), Barthel Index (to assess ADL), gait velocity, cadence and stride lenght. Measurements were performed in every patient twice: on admission (before the therapy) and after last session of the therapy to assess rehabilitation effects. RESULTS The main statistically relevant corellations observed in the study were as follows: in the whole group of patients: poor and moderate (negative) correlation between changes of gait parameters and Bobath Scale and Barthel Index, moderate and severe (negative) correlation between changes of gait parameters and Bobath Scale and Barthel Index in the group of women, correlation between changes in Bobath Scale and Barthel Index in the group of patients with left side of paresis, (negative) correlation between changes of gait parameters and Bobath Scale in group of patients younger than 68 years, moderate, high and very high correlations between changes in gait parameters in groups of women, men, younger than 68 years and older than 68 years. CONCLUSIONS There have been observed statistically significant and favourable changes in the health status of patients, described by gait parameters, changes in hand functions and ADL. Based on the presented correlations there is an assumption that it is hard to achieve simultaneous recovery in all areas: gait parameters, hand functions and ADLs in two weeks of rehabilitation.
Anaerobic activation of the entire denitrification pathway in Pseudomonas aeruginosa requires Anr, an analog of Fnr

The Pseudomonas aeruginosa gene anr, which encodes a structural and functional analog of the anaerobic regulator Fnr in Escherichia coli, was mapped to the SpeI fragment R, which is at about 59 min on the genomic map of P. aeruginosa PAO1. Wild-type P. aeruginosa PAO1 grew under anaerobic conditions with nitrate, nitrite, and nitrous oxide as alternative electron acceptors. An anr deletion mutant, PAO6261, was constructed. It was unable to grow with these alternative electron acceptors; however, its ability to denitrify was restored upon the introduction of the wild-type anr gene. In addition, the activities of two enzymes in the denitrification pathway, nitrite reductase and nitric oxide reductase, were not detectable under oxygen-limiting conditions in strain PAO6261 but were restored when complemented with the anr+ gene. These results indicate that the anr gene product plays a key role in anaerobically activating the entire denitrification pathway.
Advanced Thermal Modeling and Experimental Performance of Oil Distribution Transformers

This paper presents an analysis of the thermal behavior of a 15-kVA ONAN distribution transformer. Design, performance, and service-life improvements come into view from this study. The mathematical model, conceived as a 3-D solid-oil-environment extended system, considers all modes of heat transfer, including radiation. We computed oil velocity profile and oil and winding temperature distribution, patterns thereafter experimentally validated in accordance with the IEEE-1995 Loading Guide. Since this paper identifies hot-spot and flow patterns more accurately than its predecessors, and reveals a serious malfunction in refrigeration channels, it creates interesting possibilities for new designs in transformers below 75 kVA.
Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage

Objectives: To construct a one-plasmid expression system of the armored RNA containing long chimeric RNA byincreasing the number and affinity of the pac site. Methods: The plasmid pET-MS2-pac was constructedwith one C-variant pac site, and then the plasmid pM-CR-2C containing 1,891-bp chimeric sequences and two C-variant pac sites was produced. Meanwhile, three plasmids (pM-CR-C, pM-CR-2W and pM-CR-W) were obtained as parallel controls with a different number and affinity of the pac site. Finally, the armored RNA was expressed and purified. Results: The armored RNA with 1,891 bases target RNA was expressed successfully by the one-plasmid expression system with two C-variant pac sites, while for one pac site, no matter whether the affinity was changed or not, only the 1,200 bases target RNA was packaged. It was also found that the C-variant pac site could increase the expression efficiency of the armored RNA. The armored RNA with 1,891-bp exogenous RNA in our study showed the characterization of ribonuclease resistance and stability at different time points and temperature conditions. Conclusions: The armored RNA with 1,891 bases exogenous RNA was constructed and the expression system can be used as a platform for preparation of the armored RNA containing long RNA sequences.
Effect of organomercurials on the renal concentrating operation in hydropenic man: comments on site of action.

The renal site of action of organomercurials has variously been placed within the proximal or distal tubule on the basis of numerous observations in dog and man (1-9). Experiments in maximally hydrated man recently reported from this laboratory showed that free water clearance remained relatively fixed throughout the major portion of the diuresis induced by the organomercurial, meralluride (10). In addition, free water clearance could generally be augmented by superimposing a nonspecific solute diuretic ' during the mercurial diuresis. A hypothesis was suggested that placed a major inhibitory action of organomercurials at a late distal segment, beyond the water-clearing site where, it was assumed, a process of isosmotic salt and water reabsorption takes place. Previous data concerning the effect of mercurial diuresis on the concentrating operation in hydropenic man have led to varied conclusions. Some have argued that a mercurial diuresis limits the renal concentrating capacity (11). Others have found that a mercurial diuresis superimposed upon or developing simultaneously with a nonspecific solute diuresis does not limit the capacity of the collecting duct to extract solute-free water (12, 13). Medullary hypertonicity is established primarily by the deposition within the interstitium of salt absorbed from the ascending limb of the loop of Henle. Under conditions of maximal hydropenia, the variable hypotonicity produced in the ascending limb is dissipated by the outward diffusion of water through a permeable tubular mem-
A Novel Method for Similarity Search over Meteorological Time Series Data based on the Coulomb's Law

Several areas of knowledge use systematic and controlled observation, obtained from measurements taken at regular intervals, as a tool for behavioral analysis of phenomena, such as meteorology, which uses the observations to predict the climate behavior. Furthermore, with the advance of technology, the instruments used to measure observations have grown dramatically and the amount of data available for analysis has become greater than the ability to analyze them. In this context, this paper aims to propose a method, based on the principle of Coulomb's Law, for similarity search in time series and thus discovering intrinsic knowledge from these data. Experimental results conducted on climatic data of Brazilian cities and the sea surface temperature showed that the proposed method outperforms traditional methods on performance and accuracy and it is promising for finding similarity in series.
Development of zebrafish (Danio rerio) pectoral fin musculature

During posthatching development the fins of fishes undergo striking changes in both structure and function. In this article we examine the development of the pectoral fins from larval through adult life history stages in the zebrafish (Danio rerio), describing in detail their pectoral muscle morphology. We explore the development of muscle structure as a way to interpret the fins' role in locomotion. Genetic approaches in the zebrafish model are providing new tools for examining fin development and we take advantage of transgenic lines in which fluorescent protein is expressed in specific tissues to perform detailed three‐dimensional, in vivo fin imaging. The fin musculature of larval zebrafish is organized into two thin sheets of fibers, an abductor and adductor, one on each side of an endoskeletal disk. Through the juvenile stage the number of muscle fibers increases and muscle sheets cleave into distinct muscle subdivisions as fibers orient to the developing fin skeleton. By the end of the juvenile period the pectoral girdle and fin muscles have reoriented to take on the adult organization. We find that this change in morphology is associated with a switch of fin function from activity during axial locomotion in larvae to use in swim initiation and maneuvering in adults. The examination of pectoral fins of the zebrafish highlights the yet to be explored diversity of fin structure and function in subadult developmental stages. J. Morphol. © 2005 Wiley‐Liss, Inc.
Herpes Simplex Virus Capsid Structure: DNA Packaging Protein UL25 Is Located on the External Surface of the Capsid near the Vertices

ABSTRACT UL25 is one of seven herpes simplex virus-encoded proteins involved specifically in DNA encapsidation. Its role appears to be to stabilize the capsid so that DNA is prevented from escaping once it has entered. To clarify the function of UL25, we have examined capsids with the goal of defining where it is located. Analysis of trypsin-treated capsids showed that UL25 is sensitive to cleavage like other proteins such as the major capsid and portal proteins that are exposed on the capsid surface. Internal proteins such as the scaffolding protein and protease were not affected under the same experimental conditions. Capsids were also examined by electron microscopy after staining with gold-labeled antibody specific for UL25. Images of stained capsids demonstrated that most labeled sites (71% in C capsids) were at capsid vertices, and most stained C capsids had label at more than one vertex. A quantitative immunoblotting method showed that the capsid contents of UL25 were 56, 20, and 75 copies per capsid in A, B, and C capsids, respectively. Finally, soluble UL25 protein was found to bind in vitro to purified capsids lacking it. The amount of bound UL25 corresponded to the amount present in B capsids, and bound UL25 was found by immunoelectron microscopy to be located predominantly at the capsid vertices. The results are interpreted to suggest that five UL25 molecules are found at or near each of the capsid vertices, where they are exposed on the capsid surface. Exposure on the surface is consistent with the view that UL25 is added to the capsid as DNA is packaged or during late stages of the packaging process.
Lack of Hypoxia-Inducible Factor-1α Impairs Midbrain Neural Precursor Cells Involving Vascular Endothelial Growth Factor Signaling

Oxygen tension is critical for proliferation of human and murine midbrain-derived neural precursor cells (mNPCs). Here, we conditionally inactivated the hypoxia-responsive transcription factor hypoxia-inducible factor-1α (HIF-1α) in murine NPCs to determine its role in proliferation, survival, and dopaminergic differentiation in vitro as well as survival of murine dopaminergic neurons in vivo. HIF-1α conditional knock-out (HIF-1α CKO) mNPCs showed midbrain-specific impairment of survival and proliferation. Dopaminergic differentiation of HIF-1α CKO mNPCs in vitro was markedly reduced. Expression of vascular endothelial growth factor (VEGF) mRNA was reduced in HIF-1α CKO mNPCs, whereas erythropoietin signaling was not affected. Treatment of HIF-1α CKO mNPCs with 50 ng/ml VEGF partially recovered proliferation and dopaminergic differentiation in vitro. In substantia nigra (SN) of adult HIF-1α CKO mice, protein levels of dopaminergic marker molecules such as tyrosine hydroxylase (TH) and aldehyde dehydrogenase were reduced by 41 and 61%, respectively. The cell survival marker Bcl-2 was reduced by 58% while caspase-3 was activated. Nonbiased stereological cell counts of TH-positive neurons in SN of young adult HIF-1α CKO mice revealed a reduction of 31% compared with cre/wt mice (in which the wild- type Hif1a allele is expressed in parallel with the Cre recombinase allele). However, we found no impairment of striatal dopamine concentrations or locomotor behavior. In conclusion, HIF-1α seems to be a transcription factor relevant to the development and survival of substantia nigra dopaminergic neurons involving VEGF signaling.
Optimal power allocation for transmitter selection in MISO Rayleigh fading channels

In this paper we derive optimal power allocation (PA) in space and time that minimizes BER for transmitter selection in multi-input-single-output (MISO) Rayliegh fading channels assuming perfect channel state information (CSI) is available at receiver and transmitter. This power allocation scheme achieves exponential diversity order with a single RF front end and requires feedback of the best channel only. The presented scheme results in 7.5 dB gain over optimal single-input-single-output (SISO) power allocation scheme for 4-QAM modulation at SER of 10-4.
Spirituality, Depression, and Loneliness among Jewish Seniors Residing in New York City

This article reports the results of research that examined a randomized group of 118 Jewish seniors who were clients of one of three Jewish social service agencies in New York City. They were interviewed by four Clinical Pastoral Education residents at the Jewish Institute for Pastoral Care. During the interview, participants were asked to respond to the questions contained in the Brief Depression Scale, Version 3 of the UCLA Loneliness Scale, and the Index of Core Spiritual Experience—INSPIRIT. A statistically significant positive correlation was found between the depression and loneliness scores, r(116) = . 56, p< . 001. Spirituality was not correlated with either of these scales. Both depression and loneliness were significantly higher among women, among people who had physical impairments and those who had been victims of Nazi persecution. Depression and loneliness were inversely related to participants' ability to venture out of their house and to their relationship with their families. Having a sense of meaning or purpose in life was also inversely related to depression and loneliness. Spirituality tended to be higher among women, those participants with more years of religious education, and those with physicals impairments, but only the gender effect was statistically significant.
(TA)8 allele in the UGT1A1 gene promoter of a Caucasian with Gilbert's syndrome.

BACKGROUND AND OBJECTIVE Gilbert's syndrome, a chronic non-hemolytic unconjugated hyperbilirubinemia, is caused by a reduction in the activity of hepatic bilirubin UDP-glucuronosyltransferase (UGT1A1). This reduction has been shown to be due to a polymorphism in the promoter region of the UGT1A1 gene. The presence of seven thymine adenine (TA) repeats reduces the efficiency of transcription of the UGT1A1 gene. To elucidate the genetic background of a patient affected by Gilbert's syndrome, we collected blood samples from family members for the analysis of the A(TA)nTAA motif in the promoter region of the UGT1A1 gene. DESIGN AND METHODS Analysis of the A(TA)nTAA motif in the promoter region of the UGT1A1 gene was performed by PCR. Estimation of UGT1A1 promoter containing the variable (TA) repeats was performed by using a luciferase reporter system. RESULTS Three different genotypes were identified due to the presence of (TA)6, (TA)7 and (TA)8 repeats. The production of luciferase decreases in inverse relation to the number of repeats. INTERPRETATION AND CONCLUSIONS The (TA)7 polymorphism, associated with Gilbert syndrome, is the only allele found up to now in white populations, while two other variants (TA)5 and (TA)8 have been identified in black populations. We describe here the first case of a subject affected by Gilbert's syndrome who is heterozygous for the (TA)8 allele in the promoter region of the UGT1A1 gene. This polymorphism, as well as the (TA)7 one, is associated with an increased level of bilirubin and a significant reduction of transcription activity of the UGT1A1 gene.
Differential utilization of nuclear factor-kappaB signaling pathways for gingival epithelial cell responses to oral commensal and pathogenic bacteria.

INTRODUCTION Human beta-defensin-2 (hBD-2) is an antimicrobial peptide, induced by bacterial stimuli and inflammation, that plays a role in mucosal and skin innate immune defense. The nuclear factor-kappaB (NF-kappaB) transcription factor family is important in innate and adaptive immune responses to bacteria and proinflammatory cytokines. NF-kappaB operates via the traditional IKKbeta signaling, as well as an alternative pathway utilizing IKKalpha signaling, which is important in keratinocyte differentiation. Our previous studies showed that pathogenic, but not commensal, bacteria used NF-kappaB signaling in hBD-2 induction. The objective of this study was to understand which arm of the NF-kappaB pathway is involved in gingival epithelial cell responses to pathogenic bacteria, including hBD-2 induction. METHODS Cultured oral epithelial cells were transfected with synthetic small interfering RNAs (siRNAs) specific for various steps in each pathway, namely IKKbeta, TRAF6 and MyD88 in the canonical, and IKKalpha and TRAF3 in the alternative pathway, and subsequently stimulated with various oral bacteria. RESULTS The hBD-2 induction level was reduced to 21-61% in cells in which the alternative NF-kappaB pathway was blocked and subsequently stimulated with pathogenic bacteria, while cells in which the canonical pathway was blocked showed reduction to 78-99%. Cells stimulated with commensals showed little change in hBD-2 induction level regardless of the siRNA used. Microarray analysis showed that oral epithelia differentially regulated numerous innate immune markers in response to pathogens and commensals. CONCLUSION Our data suggest a role for the IKKalpha/TRAF3 pathway in NF-kappaB activation by pathogenic bacteria, while commensal bacteria do not utilize either NF-kappaB pathway, for hBD-2 induction.
Link Prediction Benchmarks Haifeng Qian

This document describes two temporal link prediction benchmarks that can be downloaded at [10]. Data, code for evaluation and code for several predictors are included in the packages. Section 1 describes the first benchmark, which is link prediction in coauthorship social networks and is a replication of the experiments in [6]. Performances of several predictors are also presented. Section 2 describes the second benchmark, which is link prediction in Wikipedia. Performances of several predictors are also presented. Section 3 describes formats of data files and commands to run and evaluate predictors.
Diagnosis of liver diseases using machine learning

Liver Diseases account for over 2.4% of Indian deaths per annum. [14] Liver disease is also difficult to diagnose in the early stages owing to subtle symptoms. Often the symptoms become apparent when it is too late. [1] This paper aims to improve diagnosis of liver diseases by exploring 2 methods of identification-patient parameters and genome expression. The paper also discusses the computational algorithms that can be used in the aforementioned methodology and lists demerits. It proposes methods to improve the efficiency of these algorithms.
A General Framework for the Development of MDD Projects

The main characteristic of the Model-Driven Development paradigm is that it allows the automatic (or semiautomatic) generation of software systems from the conceptual models that specify these systems. The successful application of MDD relies in the selection of appropriate diagrams and tools. However, software engineers do not have frameworks that help them to select the MDD tools that better fit to specific projects needs. To face this issue, in this paper we have analysed a set of models and tools that can be used to support MDD projects. From this analysis, a general framework is presented.
Remineralization Effect of Eggshell versus Nano-hydroxyapatite on Caries-like Lesions in Permanent Teeth (In Vitro)

Background: Eggshell (ES) is a rich source of minerals since it contains calcium, phosphorous, magnesium, strontium, and fluoride. This study sought to compare the efficacy of nano-hydroxyapatite (NHA) and ES for remineralization of enamel caries-like lesions by pH cycling. Materials and Methods: In this in vitro experimental study, artificial enamel carious lesions were induced in 47 human teeth using an acidic buffer solution. Deionized water was used as the control group, and 10% NHA and 3% and 10% ES powders in deionized water were tested as therapeutic agents. The surface microhardness of the samples was measured by a Vickers hardness tester before and after demineralization and after the application of therapeutic agents in a 7-day pH-cycling model. The data were analyzed using repeated measures analysis of variance. Results: Microhardness significantly decreased in all samples after demineralization and significantly increased after exposure to therapeutic solutions. Microhardness of the samples was not significantly different among the therapeutic groups after pH cycling. Conclusion: Based on the results of this study, ES can be used as a remineralization agent in enamel caries-like lesions.
Electrolyte and acid-base disturbances in critically ill patients : a retrospective and propensity-matched study

Background: Electrolyte and acid-base disturbances are common in critically ill patients. Their relationship with hospital mortality has not been clearly clarified. Methods: A retrospective propensity score matched analysis was used to evaluate the effects of electrolyte and acid-base disturbances on hospital mortality in critically ill patients from 2014 to 2015 in our hospital. Multiple logistic regression models and Cox proportional hazards model after propensity score matching were employed to identify their association. Results: A total of 682 critically ill patients met the inclusion criteria. The incidences of electrolyte and acid-base disturbances were higher in nonsurvivors than survivors. Many disorders occurred during hospitalization and were not corrected at discharge or before death. Patients from different categories of intensive care unit (ICU) had various distribution characteristics of electrolyte and acid-base disturbances between survivors and non-survivors. After adjusted by propensity score matching, hypouricemia, hyperuricemia, acidosis (pH<7.35) and strong ion gap >2 mmol/L at admission and hypernatremia, acidosis (pH<7.35) and AKI during hospitalization were independent risk factors for hospital mortality. Conclusions: Electrolyte and acid-base disturbances, especially hypernatremia and acidosis were highly associated with hospital mortality.