Miracle-dz/UniFAR-FTUs huggingface dataset:
Preparation, Characterization, and Properties of Novel Ti-Zr-Be-Co Bulk Metallic Glasses

We developed novel Ti-Zr-Be-Co bulk metallic glasses through Co addition based on a ternary Ti45Zr20Be35 alloy. By altering the alloying routes and alloying contents, the influence of Co alloying on glass-forming ability, thermal stability, thermoplastic formability, crystallization behavior, and corrosion resistance has been investigated systematically. It was found that the best alloying route for enhancing the glass-forming ability, thermoplastic formability, compressive plasticity, and corrosion resistance is to replace Be by Co. Ti45Zr20Be23Co12 possesses the largest critical diameter of 15 mm for glass formation. Ti45Zr20Be27Co8 possesses the highest thermoplastic formability which is comparable to that of Vitreloy alloys. Ti45Zr20Be25Co10 exhibits the largest room temperature plasticity of 15.7% together with a high specific strength of 3.90 × 105 Nm/kg. The addition of Co also strongly affects the crystallization behavior of the base alloy, resulting in a more complex crystallization process. The corrosion resistance of Ti-Zr-Be alloy in 1 mol/L HCl solution can also be enhanced by Co alloying. The related mechanisms have been explained in detail, which provide guidance for the composition design of Ti-based metallic glasses with improved properties.
Response‐surface methodology for the production and the purification of a new H2O2‐tolerant alkaline protease from Bacillus invictae AH1 strain

This work deals with the optimization of the culture conditions of Bacillus invictae AH1 in order to increase the production level of the proteolytic activity. Response‐surface methodology (RSM) was applied for the most significant fermentation parameters (concentration of wheat bran and K2HPO4/KH2PO4) that were earlier identified by Plackett–Burman Design from seven possible factors. A central composite design was used and the quadratic regression model of producing active protease was built. A maximum protease activity was reached and validated experimentally, using a maximum wheat bran concentration (50 g/L) with increased K2HPO4/KH2PO4 concentration (2.275 g/L). Protease production obtained experimentally coincident with the predicted value and the model was proven to be adequate. Interestingly, the use of RSM increased the protease production by four times (7,000 U/mL) using a low‐cost substrate and a culture time of 40 hr, as compared to the standard culture conditions. In the second part of this study, a H2O2‐tolerant alkaline protease produced from B. invictae AH1 with a molecular mass of about 41 kDa, noted P3, was purified by successive steps of ultrafiltration, gel filtration and ion exchange chromatography. The K m and Vmax values of the purified protease using casein, as substrate, were about 4 mg/mL and 27 μM/min, respectively. The highest enzyme activity was found at pH 9.0 and a temperature of 60°C. In addition, the enzyme showed a quasi‐total stability against H2O2 (5% for 1 hr) and against most of the tested solid and liquid detergents, suggesting its eventual use in bio‐detergent formulations.
Deep Sequencing Analysis Reveals Distinctive Non-Coding RNAs When Comparing Tumor Multidrug-Resistant Cells and Extracellular Vesicles with Drug-Sensitive Counterparts

Multidrug resistance (MDR) is one of the main limitations of cancer treatment. The overexpression of drug-efflux pumps, such as P-glycoprotein (P-gp), is a major cause of MDR. Importantly, different studies have shown that extracellular vesicles (EVs) participate in the communication between MDR cells and drug-sensitive counterparts, promoting dissemination of the MDR phenotype. In the present work, we aimed to identify RNA species present in MDR cells and in EVs released by those cells, which may be associated with the MDR phenotype. The RNA content from two pairs (leukemia and lung cancer) of MDR (P-gp overexpressing) cells and their drug-sensitive counterparts, as well as from their EVs, was analyzed by deep sequencing. Our results showed distinctive transcripts for MDR cells and their EVs, when compared with their drug-sensitive counterparts. Remarkably, two pseudogenes (a novel pseudogene and RNA 5.8S ribosomal pseudogene 2) were found to be increased in EVs released by MDR cells in both leukemia and lung cancer models. Moreover, six miRs (miR-204-5p, miR-139-5p, miR-29c-5p, miR-551b-3p, miR-29b-2-5p, and miR-204-3p) exhibited altered levels in lung cancer MDR cells and their EVs. This study provides insights into the contribution of EVs to MDR.
No Acute Effects of Exogenous Glucose-Dependent Insulinotropic Polypeptide on Energy Intake, Appetite, or Energy Expenditure When Added to Treatment With a Long-Acting Glucagon-Like Peptide 1 Receptor Agonist in Men With Type 2 Diabetes

OBJECTIVE Dual incretin receptor agonists in clinical development have shown reductions in body weight and hemoglobin A1c (HbA1c) in patients with type 2 diabetes, but the impact of glucose-dependent insulinotropic polypeptide (GIP) receptor activation remains unclear. Here, we evaluated the effects of high-dose exogenous GIP on energy intake, energy expenditure, plasma glucose, and glucose-regulating hormones in patients with type 2 diabetes treated with a long-acting glucagon-like peptide 1 receptor (GLP-1R) agonist. RESEARCH DESIGN AND METHODS In a randomized, double-blind design, men with type 2 diabetes (n = 22, mean ± SEM HbA1c 6.8 ± 0.1% [51 ± 1.5 mmol/mol]) treated with metformin and long-acting GLP-1R agonists were subjected to two 5-h continuous infusions (separated by a washout period of ≥3 days): one with GIP (6 pmol/kg/min) and another with saline (placebo). After 60 min of infusion, a liquid mixed-meal test was performed, and after 270 min of infusion, an ad libitum meal was served for evaluation of energy intake (primary end point). RESULTS Energy intake was similar during GIP and placebo infusion (648 ± 74 kcal vs. 594 ± 55 kcal, respectively; P = 0.480), as were appetite measures and energy expenditure. Plasma glucagon and glucose were higher during GIP infusion compared with placebo infusion (P = 0.026 and P = 0.017) as assessed by area under the curve. CONCLUSIONS In patients with type 2 diabetes, GIP infusion on top of treatment with metformin and a long-acting GLP-1R agonist did not affect energy intake, appetite, or energy expenditure but increased plasma glucose compared with placebo. These results indicate no acute beneficial effects of combining GIP and GLP-1.
The Penetration of Internet of Things in Robotics: Towards a Web of Robotic Things

As the Internet of Things (IoT) penetrates different domains and application areas, it has recently entered also the world of robotics. Robotics constitutes a modern and fast-evolving technology, increasingly being used in industrial, commercial and domestic settings. IoT, together with the Web of Things (WoT) could provide many benefits to robotic systems. Some of the benefits of IoT in robotics have been discussed in related work. This paper moves one step further, studying the actual current use of IoT in robotics, through various real-world examples encountered through a bibliographic research. The paper also examines the potential of WoT, together with robotic systems, investigating which concepts, characteristics, architectures, hardware, software and communication methods of IoT are used in existing robotic systems, which sensors and actions are incorporated in IoT-based robots, as well as in which application areas. Finally, the current application of WoT in robotics is examined and discussed.
Protective Effect of Zizyphus lotus L. (Desf.) Fruit against CCl4-Induced Acute Liver Injury in Rat

In Morocco, “Zizyphus lotus L. ” is one of the most widely and traditionally used plant species to treat various diseases, including liver disorders. The present study was conducted to evaluate the aqueous extract of Zizyphus lotus L. fruit against carbon tetrachloride- (CCL4-) induced liver damage in Wistar rats. The animals were daily treated with the aqueous extract of Zizyphus lotus L. fruit using two doses separately 200 and 400 mg/kg body weight for 14 days. CCL4 was injected intraperitoneally (1 ml/kg body weight) at two times, 7th and 14th days. At the end of the treatment, rats were sacrificed, and the blood was collected for the assessment of biochemical parameters. Moreover, the body weight as well as liver weight was determined. The injection of CCl4 to the rats induced various alterations such as the increase of relative liver weight, serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, triglycerides, very low-density lipoproteins, total cholesterol (slight increase), creatinine, urea, uric acid, and malondialdehyde. On the contrary, the effects of CCL4 lead to the reduction in serum levels of high-density lipoprotein. However, the daily administration of the aqueous extract of Zizyphus lotus L. fruit to the injected rats with CCL4 restored this abnormal variation in these biochemical parameters to normal values. Based on the results obtained in this study, it seems that the aqueous extract of Zizyphus lotus L. fruit has an hepatoprotective effect against hepatic lesions induced by CCL4 in rats.
Reducing Amplitude Fluctuation by Gradual Phase Shift in Midair Ultrasound Haptics

Ultrasound emitted from an array of transducers can produce various tactile sensations by temporally controlling the phase and amplitude of the transducers. However, the controllability in haptic applications has not been well examined. This article clarifies a phase shift of the driving signal causes amplitude fluctuation of emitted ultrasound, even under a constant driving amplitude. We demonstrate theoretically that this problem exists in general resonant systems with various quality factors, and point out that it produces radiation force decrease and audible noise. We also show a method to reduce the fluctuation and quantitatively evaluate the effectiveness. The results provide the measure of the displayed force fluctuation by a fast focus movement and enable silent haptic stimulation.
Indonesian Version of Addiction Rating Scale of Smartphone Usage Adapted from Smartphone Addiction Scale-Short Version (SAS-SV) In Junior High School

BACKGROUND: The increase of smartphone user among Indonesian junior high school students, particularly for teenagers, indicates the addiction factor of the smartphone. AIM: This research is designed to adapt the Smartphone Addiction Scale-Short Version (SASS-SV) to Indonesian version based on cultural adaptation of the rating scale. METHODS: This study involves 300 participants consisted of 151 of male children and 149 female children with an average age is 13.27 years-of-old. The validity of concurrent was used to obtain the validity, while the internal consistency and Receiver Operating Characteristic (ROC) were conducted to confirm the reliability of the rating scale. RESULTS: In purpose to measure internal consistency, the Cronbach alpha has been applied. The Cronbach alpha value was 0.740, and Concurrent validity was checked to NMP the Nomophobia Questionnaire (NMP-Q) based on Indonesian version. The analysis of ROC showed that the value of Area under the Curve (AUC) was 0.997 (0.990-1.000), with cut-off value accounted for ≥ 32, a sensitivity value of 0.91 and specificity value was 0.973 for the male children. On the hand of female children, the results showed similarity with the AUC was 0.996 (0.998-1.000), and the cut-off, sensitivity and specificity values were accounted for ≥ 34, 0.91, and 0.974 respectively. CONCLUSION: The Indonesian version of SAS-SV provided acceptable validation results as well as the reliability, and this version can be used to evaluate the smartphone addiction in Indonesia.
Invertible Generative Modeling using Linear Rational Splines

Normalizing flows attempt to model an arbitrary probability distribution through a set of invertible mappings. These transformations are required to achieve a tractable Jacobian determinant that can be used in high-dimensional scenarios. The first normalizing flow designs used coupling layer mappings built upon affine transformations. The significant advantage of such models is their easy-to-compute inverse. Nevertheless, making use of affine transformations may limit the expressiveness of such models. Recently, invertible piecewise polynomial functions as a replacement for affine transformations have attracted attention. However, these methods require solving a polynomial equation to calculate their inverse. In this paper, we explore using linear rational splines as a replacement for affine transformations used in coupling layers. Besides having a straightforward inverse, inference and generation have similar cost and architecture in this method. Moreover, simulation results demonstrate the competitiveness of this approach's performance compared to existing methods.
Selective Targeting of Cancer Stem Cells (CSCs) Based on Photodynamic Therapy (PDT) Penetration Depth Inhibits Colon Polyp Formation in Mice

Targeting cancer stem cells (CSCs) without damaging normal stem cells could contribute to the development of novel radical cancer therapies. Cells expressing leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) constitute a cancer-causing population in the colon; therefore, targeting of Lgr5+ cells is expected to provide an opportunity to mitigate colon cancer. However, the expression of Lgr5 in normal stem cells makes it difficult to prove the efficacy of therapies targeted exclusively at Lgr5+ cancer cells. We used a modified photodynamic therapy technique involving cellular radiative transfer between green fluorescent protein (GFP)-expressing cells and a rose bengal photosensitizer. After treatment, tumors containing GFP-Lgr5+ cells were observed to be significantly suppressed or retarded with little effect on GFP-Lgr5+ stem cells at the crypt bottom. Lgr5+ CSCs were specifically eradicated in situ, when localized based on the depth from the colon lumen, revealing the potential preventive efficacy of Lgr5-targeted therapy on tumor growth. This study supports the idea that Lgr5+ cells localized near the colon luminal surface are central to colorectal cancer. With further development, the targeting of localized Lgr5+ cancer stem cells, which this study demonstrates in concept, may be feasible for prevention of colon cancer in high-risk populations.
One-Fifth of Children with Sickle Cell Anemia Show Exercise-Induced Hemoglobin Desaturation: Rate of Perceived Exertion and Role of Blood Rheology

Perceived exertion is an important self-limiting factor influencing functional capacity in patients with sickle cell anemia (SCA). Exercise-related hemoglobin desaturation (EHD) may occur during a six-minute walking test (6MWT) and could influence the perceived rate of exertion. The aims of this study were (1) to compare the 6MWT responses (heart rate, perceived rate of exertion, and distance covered) between SCA children with and without EHD, and (2) to test the associations between EHD and several biological/physiological parameters. Nine of 51 SCA children (18%) at steady state (mean age 11.9 ± 3.8 years) exhibited EHD at the end of the 6MWT. The rate of perceived exertion increased with exercise in the two groups, but reached higher values in the EHD group. Heart rate and performance during the 6MWT did not differ between the two groups. The magnitude of change in SpO2 during the 6MWT was independently associated with the red blood cell (RBC) deformability and RBC aggregates strength. This study demonstrates that SCA children with EHD during a 6MWT have a higher rate of perceived exertion than non-EHD children despite a similar physiological demand, and that abnormal RBC rheology determinants appear to be significant contributors.
Metamaterial Cell-Based Superstrate towards Bandwidth and Gain Enhancement of Quad-Band CPW-Fed Antenna for Wireless Applications

A multiband coplanar waveguide (CPW)-fed antenna loaded with metamaterial unit cell for GSM900, WLAN, LTE-A, and 5G Wi-Fi applications is presented in this paper. The proposed metamaterial structure is a combination of various symmetric split-ring resonators (SSRR) and its characteristics were investigated for two major axes directions at (x and y-axis) wave propagation through the material. For x-axis wave propagation, it indicates a wide range of negative refractive index in the frequency span of 2–8.5 GHz. For y-axis wave propagation, it shows more than 2 GHz bandwidth of near-zero refractive index (NZRI) property. Two categories of the proposed metamaterial plane were applied to enhance the bandwidth and gain. The measured reflection coefficient (S11) demonstrated significant bandwidths increase at the upper bands by 4.92–6.49 GHz and 3.251–4.324 GHz, considered as a rise of 71.4% and 168%, respectively, against the proposed antenna without using metamaterial. Besides being high bandwidth achieving, the proposed antenna radiates bi-directionally with 95% as the maximum radiation efficiency. Moreover, the maximum measured gain reaches 6.74 dBi by a 92.57% improvement compared with the antenna without using metamaterial. The simulation and measurement results of the proposed antenna show good agreement.
Aggregation of CAT tails blocks their degradation and causes proteotoxicity in S. cerevisiae

The Ribosome-associated Quality Control (RQC) pathway co-translationally marks incomplete polypeptides from stalled translation with two signals that trigger their proteasome-mediated degradation. The E3 ligase Ltn1 adds ubiquitin and Rqc2 directs the large ribosomal subunit to append carboxy-terminal alanine and threonine residues (CAT tails). When excessive amounts of incomplete polypeptides evade Ltn1, CAT-tailed proteins accumulate and can self-associate into aggregates. CAT tail aggregation has been hypothesized to either protect cells by sequestering potentially toxic incomplete polypeptides or harm cells by disrupting protein homeostasis. To distinguish between these possibilities, we modulated CAT tail aggregation in Saccharomyces cerevisiae with genetic and chemical tools to analyze CAT tails in aggregated and un-aggregated states. We found that enhancing CAT tail aggregation induces proteotoxic stress and antagonizes degradation of CAT-tailed proteins, while inhibiting aggregation reverses these effects. Our findings suggest that CAT tail aggregation harms RQC-compromised cells and that preventing aggregation can mitigate this toxicity.
Acoustic Patterning of Growth Factor for 3D Tissue Engineering.

Temporal and spatial presentations of biological cues are critical for tissue engineering. There is a great need in improving the incorporation of bioagent(s) (specifically growth factor(s)) onto three-dimensional (3D) scaffolds. In this study, we developed a process to combine additive manufacturing technology with acoustic droplet ejection (ADE) technology to control growth factor distribution. More specifically, we implemented ADE to control the distribution of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) onto polycaprolactone (PCL)-based tissue engineering constructs. Three substrates were used in this study: (1) succinimide-terminated PCL (PCL-NHS) as model material, (2)alkali-treated PCL (PCL-NaOH) as first control material, and (3) fibrin-coated PCL (PCL-Fibrin) as second control material. It was shown that our process enables a pattern of BMP-2 spots of ~250 µm in diameter with ~700 µm center-to-center spacing. An initial concentration of BMP-2 higher than 300 µg/L was required to retain a detectable amount of growth factor on the substrate after a wash with phosphate buffer solution. However, to obtain detectable osteogenic differentiation of C2C12 cells, the initial concentration of BMP-2 higher than 750 µg/L was needed. The cells on PCL-NHS samples showed spatial alkaline phosphatase staining correlating with local patterns of BMP-2 although the intensity was lower than the controls (PCL-NaOH and PCL-Fibrin). Our results have demonstrated that the developed AM-ADE process holds great promise in creating tissue engineering constructs with highly controlled growth factor patterning.
Inference of gene regulatory networks based on nonlinear ordinary differential equations

MOTIVATION Gene regulatory networks capture the regulatory interactions between genes, resulting from the fundamental biological process of transcription and translation. In some cases, the topology of GRNs is not known, and has to be inferred from gene expression data. Most of the existing GRNs reconstruction algorithms are either applied to time-series data or steady-state data. Although time-series data include more information about the system dynamics, steady-state data imply stability of the underlying regulatory networks. RESULTS In this paper, we propose a method for inferring GRNs from time-series data and steady-state data jointly. We make use of a nonlinear ordinary differential equations framework to model dynamic gene regulation and an importance measurement strategy to infer all putative regulatory links efficiently. The proposed method is evaluated extensively on the artificial DREAM4 dataset and two real gene expression datasets of yeast and Escherichia coli. Based on public benchmark datasets, the proposed method outperforms other popular inference algorithms in terms of overall score. By comparing the performance on the datasets with different scales, the results show that our method still keeps good robustness and accuracy at a low computational complexity. AVAILABILITY AND IMPLEMENTATION The proposed method is written in the Python language, and is available at: https://github.com/lab319/GRNs_nonlinear_ODEs. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
Soluble Fiber and Insoluble Fiber Regulate Colonic Microbiota and Barrier Function in a Piglet Model

The main purpose of the present study was to assess the effect of soluble and insoluble fiber on colonic bacteria and intestinal barrier function in a piglet model. A total of 24 piglets (25 ± 1 d old; 7.50 ± 0.31 kg) were randomly allotted to 4 treatments: basal diet (control, CON), 1% insoluble dietary fiber (IDF) diet, 1% soluble dietary fiber (SDF) diet, and 0.5% insoluble fiber + 0.5% soluble dietary fiber (MDF) diet. The trial lasted 28 days. SDF-fed piglets showed a higher (P < 0.05) bacterial a-diversity (observed_species, chao1, and ACE) and a higher relative abundance of Proteobacteria and Actinobacteria, Solobacterium, Succinivibrio, Blautia, and Atopobium in colonic digesta than CON, IDF, and MDF groups (P < 0.05). At the same time, Bacteroidetes, Euryarchaeota, Phascolarctobacterium, Coprococcus_1, and Prevotella_1 were significantly increased in the IDF group when compared with CON, SDF, and MDF groups (P < 0.05). Furthermore, Bacteroidetes and Enterobacteriaceae, Selenomonas, Phascolarctobacterium, and Alloprevotella(P < 0.05) were significantly higher in the MDF group than those in the other three groups (P < 0.05). SDF diet increased the concentrations of short-chain fatty acid (SCFA) in colonic digesta (P < 0.05) when compared with the CON group and enhanced weight index of the colon (P < 0.05) than the CON and IDF groups. Furthermore, compared with the CON group, SDF, IDF, and MDF diets all upregulated the mRNA expressions of claudin-1 (CLDN-1) in colonic mucosa (P < 0.05), SDF and IDF diets upregulated the mRNA expressions of mucin 2 (MUC2) (P < 0.05), SDF diet increased mRNA expressions of zonula occludens 1 (ZO-1) and occludin (OCLN), while the IDF group enhanced the secretory immunoglobulin A (sIgA) concentrations (P < 0.05), respectively. IDF and MDF diets decreased expressions of TNF-α(P < 0.05). We concluded that the influence of soluble fiber on colonic microbiota was more extensive than that of insoluble fiber. Moreover, soluble fiber could more effectively improve colonic barrier function by upregulating gene expressions of the gut barrier.