Miracle-dz/UniFAR-FTUs huggingface dataset:
Cloning and characterization of GPD2, a second gene encoding sn‐glycerol 3‐phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae, and its comparison with GPD1

We have cloned and characterized a homologue of the previously isolated GPD1 gene, encoding sn‐glycerol 3‐phosphate dehydrogenase (NAD+) in Saccharomyces cerevisiae. This second gene, called GPD2, encodes a protein of 384 amino acids that shares 69% sequence identity with GPD1. Like GPD1 it has an amino‐terminal extension of unknown function. GPD2 is located on chromosome VII and cross‐hybridizes with GPD1 at chromosome IV as well as with an unknown homologue at chromosome XV. Disruption of the GPD2 gene did not reveal any observable phenotypic effects, whereas overexpression resulted in a slight, but significant, increase of GPD enzyme activity in wild‐type cells. Analysis of gene transcription by a CAT‐reporter gene fused to the GPD promoters revealed decreased transcriptional activity of the GPD2 promoter in cells grown on non‐fermentable as opposed to fermentable carbon sources, and no induction in cells exposed to high osmolarity or heat shock. Similar analysis of GPD1 demonstrated an 8–17‐fold higher basal level of transcription compared to GPD2. Furthermore, such analysis revealed that the GPD1 promoter was it induced by increased osmolarity essentially independent of the type of stress solute used, the level of GPD1 transcription being increased about sevenfold in cells growing at 1.4M NaCl.
Dental Autoradiography I. Choice of Radioisotope

N earlier publication dealt with posteruptive changes of teeth in relation 1A, to the resistance of the tooth to decay. ' Radioisotope uptake was used as a tool for investigation in many of the studies discussed. During the past two years, in continuation of earlier studies of posteruptive changes, autoradiography has been used to supplement other histopathologic technics for the study of the structure of extracted teeth. A simple and satisfactory method for the purpose has been developed, and the findings from its use have proved valuable. They offer information regarding the permeability of the tooth to aqueous solutions in greater measure than that which is available from the usual staining and photographic methods. The technics employed already have been described in general terms by others,2 or are adaptations of common procedures. Yet believing that a description of our methods and findings will be of value to other workers in related fields of investigation, it is planned to describe them in a series of brief presentations, each dealing with specific aspects of the autoradiographic recording of dental findings. The current report will deal with general methodology and with the basis for the choice of specific radioisotopes as tools for study. Searching for an improved method for the measurement of dentinal sclerosis, it was planned to use the capacity for radioisotope uptake as a measure of the internal space of the tooth. To avoid uptake secondary to ion exchange, radioiodine first was used. For purpose of comparison, some autoradiograms also were made using P32, recognizing that this element would become fixed within the dental structure through ion exchange as well as through adsorption. Neither the radioiodine nor the radiophosphorus gave autoradiograms with sharp histologic detail. It was reasoned that the blurring that might be expected from the emission of long range beta particles or gamma rays from the lower layers of the dental structure would serve to obscure the registry on the film of the radiant energy being emitted from the surface of the specimen. As a consequence, radiosulfur was tried. This isotope gives off its radiant energy only in the form of beta particles, and the range of these particles in the case of radiosulfur is slight; they can penetrate a maximum thickness of only 0.2 mm. of water, and about half that thickness of dentin. The autoradiograms obtained through the use of S35 are markedly sharper in
Structural and spectroscopic investigations of CdS/HgS/CdS quantum-dot quantum wells.

Epitaxial growth in a CdS/HgS heterostructure of nanometer dimensions, prepared by methods of wet chemistry, is demonstrated. High-resolution transmission-electron microscopy is used to determine the shape and crystallinity of this system consisting of a quantum well in a quantum dot. The homogeneous absorption and fluorescence spectra are investigated by transient hole burning and fluorescence line-narrowing spectroscopy. The photophysical measurements provide evidence for charge-carrier localization within the HgS well. {copyright} {ital 1996 The American Physical Society. }
ENHANCED WARM H2 EMISSION IN THE COMPACT GROUP MID-INFRARED “GREEN VALLEY”

We present results from a Spitzer mid-infrared spectroscopy study of a sample of 74 galaxies located in 23 Hickson Compact Groups (HCGs), chosen to be at a dynamically active stage of H i depletion. We find evidence for enhanced warm H2 emission (i.e., above that associated with UV excitation in star-forming regions) in 14 galaxies (∼20%), with 8 galaxies having extreme values of L(H2 S(0)-S(3))/L(7.7 μm polycyclic aromatic hydrocarbon), in excess of 0.07. Such emission has been seen previously in the compact group HCG 92 (Stephan's Quintet), and was shown to be associated with the dissipation of mechanical energy associated with a large-scale shock caused when one group member collided, at high velocity, with tidal debris in the intragroup medium. Similarly, shock excitation or turbulent heating is likely responsible for the enhanced H2 emission in the compact group galaxies, since other sources of heating (UV or X-ray excitation from star formation or active galactic nuclei) are insufficient to account for the observed emission. The group galaxies fall predominantly in a region of mid-infrared color–color space identified by previous studies as being connected to rapid transformations in HCG galaxy evolution. Furthermore, the majority of H2-enhanced galaxies lie in the optical “green valley” between the blue cloud and red sequence, and are primarily early-type disk systems. We suggest that H2-enhanced systems may represent a specific phase in the evolution of galaxies in dense environments and provide new insight into mechanisms which transform galaxies onto the optical red sequence.
Analysis of Flight Data Using Clustering Techniques for Detecting Abnormal Operations

The airline industry is moving toward proactive risk management, which aims to identify and mitigate risks before accidents occur. However, existing methods for such efforts are limited. They rely on predefined criteria to identify risks, leaving emergent issues undetected. This paper presents a new method, cluster-based anomaly detection to detect abnormal flights, which can support domain experts in detecting anomalies and associated risks from routine airline operations. The new method, enabled by data from the flight data recorder, applies clustering techniques to detect abnormal flights of unique data patterns. Compared with existing methods, the new method no longer requires predefined criteria or domain knowledge. Tests were conducted using two sets of operational data consisting of 365 B777 flights and 25,519 A320 flights. The performance of cluster-based anomaly detection to detect abnormal flights was compared with those of multiple kernel anomaly detection, which is another data-driven anomaly .
Highly‐efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants

Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for the preparation of genomic DNA (gDNA) template, which could be directly used for PCR, was developed. Cells from colonies or liquid cultures were collected and sequentially treated by microwave, plMAN5C, proteinase K and boiling (MMPB) in a single tube to give cell lysates that were qualified as PCR templates. Single‐copied gDNA fragments o up to 2.8 kb were successfully amplified. We also demonstrated successful application of this method for species in the Ascomycetes and Basidiomycetes and identification of two leucine auxotroph mutants of Rhodotorula glutinis. This method could be widely employed for the screening and genetic engineering of various yeasts. Copyright © 2012 John Wiley & Sons, Ltd.
Communication theory and coding for channels with intersymbol interference

This thesis is primarily concerned with the analysis of discrete-time communication channels with intersymbol interference (ISI) and addi¬ tive white Gaussian noise (AWGN). Complex random variables and processes are useful for describing the baseband equivalent of bandpass communication channels with memory and as shown in this thesis for deriving the capacity of ISI channels with a complex unit-sample response and complex AWGN. The com¬ plex noise process in the equivalent baseband channel usually exhibits certain symmetries in the autocovariance and crosscovariance function of its real and imaginary part. We show that in order to achieve ca¬ pacity the information-carrying process must obey the same covariance symmetries. It is shown that the 'covariance' of complex random variables and processes, when denned consistently with the corresponding notion for real random variables, is specified by the conventional (complex) covari¬ ance and a quantity called the pseudo-covariance. A characterization of uncorrelatedness and wide-sense stationarity in terms of covariance and pseudo-covariance is given. The above covariance symmetries corre¬ spond to a vanishing pseudo-covariance. Complex random variables and processes with a zero pseudo-covariance are called proper. It is shown that properness is preserved under affine transformations and that the complex-multivariate Gaussian density assumes a natural form only for proper random variables. The maximum-entropy theorem is generalized to the complex-multivariate case; the differential entropy of a complex random vector with a fixed correlation matrix is shown to be maximum if and only if the random vector is proper, Gaussian and zero-mean. The notion of circular stationarity is introduced. For proper complex ran¬ dom variables, a discrete Fourier transform correspondence is derived that relates circular stationarity in the time domain to uncorrelatedness
EFFICACY OF A PROPRIETARY IBUPROFEN GEL IN SOFT TISSUE INJURIES: A RANDOMISED, DOUBLE‐BLIND, PLACEBO‐CONTROLLED STUDY

The efficacy of a novel, proprietary topical formulation of ibuprofen 5% gel (Ibugel™) was evaluated in a placebo‐controlled study in patients with soft tissue injuries. Patients received either active gel (n=40) or placebo gel (n=41) for a maximum of seven days. Pain and interference with physical activity were assessed daily using visual analogue scales. There was a significant difference (p<0.001) in favour of active treatment for the time to achieve clinically meaningful reduction in pain. By day 7, 75% of patients in the active gel group had a clinically meaningful reduction of pain compared with 39% of patients who received placebo. Despite differences between study centres, the data for interference with physical activity also showed an advantage for active treatment. By day 7, 79% of patients in the active gel group had a clinically meaningful reduction in interference with physical activity, compared with 44% of patients who received placebo.
Biohydrogen Production by Anaerobic Fermentation of Sewage Sludge - Effect of Initial pH

The aim of this study is to investigate the usability of sewage sludge, a waste from waste water treatment facility, at constant temperature and different pH conditions in the hydrogen production by dark fermentation. It was understood from the results that hydrogen production varies according to the characterization of activated sludge. In the experiments performed at different pH values (pH 4-8) at 35℃, maximum hydrogen production was achieved within the first 24 hours. Except for one performed at pH 8, hydrogen gas release was observed at other pH values within 2 hours. However, according to the results obtained at the 24th hour, maximum hydrogen production (2489 mL/m 3 H 2 ) was observed at pH 8.
Data Mining Engine using Predictive Analytics

analytics is a field of data mining which extracts information from the past and use it to predict the future trends. This paper establishes the importance of predictive analysis. In this paper, we present a system to analyse user stories incorporating the data of energy and health demands of four countries - namely India, China, United States of America and Brazil; for the past 30 years, depict them graphically using Business Intelligence and finally predict the future trend of the parameters. The correlation between various entities is found out using Pearson's coefficient. Finally we can see the predicted values of 30-40 years ahead and predict the emerging trends in the form of Power View charts. We present lessons learned and future directions for improving the user in the loop workflow for predictive analytics. KeywordAnalytics, Regression Analysis, Health and Energy Demands
Mechanical Properties and Microstructural Properties of CrSiN Coating

The present study deals with the characterization of CrSiN coatings obtained by PVD magnetron sputtering systems. CrSiN films were deposited with different Si contents, in order to check the effect of at. % variation on the different properties of the Cr–N system. Coatings were characterized by scanning electron microscopy (SEM) for thickness measurements, X-ray diffraction. Surface morphology and the roughness characteristics were explored using AFM, Mechanicals properties, elastic and plastic deformation resistance of thin films were investigated using nanoindentation test. We observed that the Si addition improved the hardness and the Young’s modulus of the Cr–N system. Indeed, the hardness value is 18,56 GPa for CrSiN coatings. Besides, the Young’s modulus value is 224,22 GPa for CrSiN coatings for Si content of 1.2 at. %. Keywords—Thin film, mechanicals properties, PVD.
Institutional drivers and barriers to faculty adoption of blended learning in higher education

Relatively little research on blended learning ( BL) addresses institutional adoption, although such research would benefit institutions of higher education in strategically adopting and implementing BL. In a prior study, the authors proposed a framework for institutional BL adoption, identifying three stages: (1) awareness/exploration, (2) adoption/early implementation, and (3) mature implementation/growth. The framework also identified key strategy, structure and support issues universities may address at each stage. The current study applies that framework as well as Rogers' diffusion of innovations theory to determine the degree to which institutional strategy, structure and support decisions facilitate or impede BL adoption among higher education faculty. The authors also explore whether faculty members' innovation adoption category (innovator, early adopter, early majority, late majority or laggard) affects which decisions facilitate or impede BL adoption. To achieve these objectives, the authors surveyed 214 faculty members at a university in the adoption/early implementation stage, Brigham Young University-Idaho. We found that the availability of sufficient infrastructure, technological support, pedagogical support, evaluation data and an institution's purpose for adopting BL would most significantly influence faculty adoption. We also identified a wide range of factors that would influence each category of innovation adopters. [ABSTRACT FROM AUTHOR]
CONOPS and autonomy recommendations for VTOL small unmanned aerial system based on Hurricane Katrina operations

This field study examines vertical takeoff and landing (VTOL) small unmanned aerial system (SUAS) operations conducted as part of an 8‐day structural inspection task following Hurricane Katrina in 2005. From the observations of the 32 flights spread over 12 missions, four key findings are identified for concept of operations (CONOPS) and the next level of artificial intelligence for rotary‐wing SUASs operating in cluttered urban environments. These findings are (1) the minimum useful standoff distance from inspected structures is 2–5 m, (2) omnidirectional sensor capabilities are needed for obstacle avoidance, (3) global positioning system waypoint navigation is unnecessary, and (4) these operations require three operators for one SUAS. Based on the findings and other observations, a crewing organization and flight operations protocol for SUASs are proposed. Needed directions in research and development are also discussed. These recommendations are expected to contribute to the design of platforms, sensors, and artificial intelligence as well as facilitate the acceptance of SUASs in the workplace. © 2009 Wiley Periodicals, Inc.
FPGA implementation of multi-band spectral subtraction method for speech enhancement

This paper proposes a hardware architecture of the multi-band spectral subtraction method for real-time speech enhancement. The proposed hardware architecture has been implemented on field programmable gate array (FPGA) device using Xilinx system generator (XSG) and Nexys-4 development board. Multi-band approach is based on the fact the whole speech spectrum does not be affected uniformly by the colored noise. Speech enhancement performances obtained by the hardware architecture are compared to those obtained by MATLAB simulation. The resource utilization and the maximum operating frequency are reported for an Artix-7 FPGA chip.
AIDS-Related Pancreatic BurkittS Lymphoma. EUS-FNA Enhanced Diagnosis with Fluorescence in Situ Hybridization (FISH)

Context Non-Hodgkin's lymphoma is a common complication in HIV-patients that most frequently affects the gastrointestinal tract. Primary pancreatic lymphomas and Burkitt Lymphoma involving the pancreas are uncommon. It is important to recognize them because can mimic an adenocarcinoma or pancreatitis, but their management is completely different. Case report We report a case of a forty-seven-year-old man who presented with an AIDS-related Burkitt Lymphoma with acute pancreatitis as initial manifestation. Initially patient was admitted with abdominal pain and high amylase levels. Computed tomography imaging was suggestive of acute pancreatitis. Later was found to be human immunodeficiency virus seropositive. 4-weeks later, a control computed tomography scan revealed growth of a well-defined large pancreatic mass, with diffuse enlargement of the gland, and a normal-appearing pancreatic duct. Consequently an endoscopic ultrasound-guided fine needle aspiration was performed with a 19-gauge needle and revealed a proliferation of medium lymphocytes, inconspicuous cytoplasm and frequent mitosis. The lymphocytes were positive for CD20 and CD10. The Ki-67 labeling index was almost 80%. BCL-2 and MYC FISH molecular analysis was performed and confirmed t(8;14)(q24;q32). On the basis of these results, pancreatic Burkitt's lymphoma was diagnosed. Positive emission tomography scan completed staging and showed uptake in the pancreas and multiple metastasis. Accordingly patient received chemotherapy by PHETEMA BURKIMAB protocol, obtaining complete remission. Conclusion Pancreatic Lymphoma should be considered in differential diagnosis of pancreatic masses. EUS-FNA including flow cytometry and molecular analysis are useful techniques that may help to establish early diagnosis and prompt treatment avoiding unnecessary surgery.
Guarding 1.5D terrains with demands

In this paper, we consider the 1.5D terrain guarding problem in which every point on the terrain that is to be covered has an integer demand associated with it. The goal is to find a minimum cardinality set of guards such that each point is guarded by a number of guards satisfying its demand. We present a first constant-factor approximation algorithm for the problem, that is, a (1+1/d min)-approximation algorithm, where d min is a minimum demand. The algorithm is based on solving appropriate subproblems established by a decomposition of the main problem due to linear programming relaxation of the corresponding covering problem.
Economic impact and quality of life as endpoints of nutritional therapy

Purpose of reviewThe present review exposes why considering primary endpoints such as cost-effectiveness and quality of life in wasting disease research is critical for promoting medical nutrition therapy. Recent findingsDespite growing evidence that nutritional support improves patients' clinical outcome, its use is not widely considered as a routine by most healthcare professionals. Many factors, depending on physicians, patients and institutions, could explain such a resistance to implement nutritional therapy in routine care. One of these factors is the lack of indisputable evidence that nutritional intervention improves patients' quality of life and is cost-effective. SummaryIn today's resource-constrained environment, disease management strategies are judged in terms of not only clinical efficacy and safety but also patient satisfaction and economic dimensions. The demonstration of a positive costs/saving ratio is crucial to obtain the political backing of health administrators and sustain further investment in research. Moreover, improving patients' quality of life promotes their capacity to cope with psychological distress, increases their tolerance and response to treatments, and enhances the global image of the healthcare system. In wasting diseases research, there is a need for well designed clinical trials from which cost-utility performance of nutritional interventions could be assessed in order to convince all the stakeholders and to get support from clinicians and patients themselves.
Ana4: a 2.5 Framework for Deploying Real Multi-hop Ad hoc and Mesh Networks

We consider the problem of interconnecting several hosts in a spontaneous hybrid and heterogeneous network, i.e. an environment where wired and multi-hop wireless technologies are used. Dealing with issues raised by such hybrid networks and addressing all challenges listed in MANet may require a departure from classical solutions available in the literature. To enable a full multi-hop connectivity without raising problems/inconsistencies regarding IP compatibility, we propose Ana4 (a.k.a.Ananas), a 2.5 layer architecture suitable for hybrid heterogeneous networks. In this article, we present the architecture and implementations of Ana4 in the context of ad hoc and mesh networks as well as application fields where Ana4 is already used. We also present experimental measurements that show good performances with respect to a full IP solution (IP routing) and similar 2.5 approaches like MPLS.
Event-Based Impulsive Control of Continuous-Time Dynamic Systems and Its Application to Synchronization of Memristive Neural Networks

This paper investigates exponential stabilization of continuous-time dynamic systems (CDSs) via event-based impulsive control (EIC) approaches, where the impulsive instants are determined by certain state-dependent triggering condition. The global exponential stability criteria via EIC are derived for nonlinear and linear CDSs, respectively. It is also shown that there is no Zeno-behavior for the concerned closed loop control system. In addition, the developed event-based impulsive scheme is applied to the synchronization problem of master and slave memristive neural networks. Furthermore, a self-triggered impulsive control scheme is developed to avoid continuous communication between the master system and slave system. Finally, two numerical simulation examples are presented to illustrate the effectiveness of the proposed event-based impulsive controllers.