Miracle-dz/UniFAR-FTUs huggingface dataset:
Can the speed limit be used as a surrogate for the striking vehicle’s travel speed or delta-V in police-reported rear-end crashes?

Abstract Objective: A recent study by Kidd (2022) recommended that organizations evaluating front crash prevention (FCP) systems like automatic emergency braking and forward collision warning increase speed differentials in existing test scenarios from 25 mph to 45 mph to make the tests more representative of police-reported rear-end crashes. Kidd used the posted speed limit as a proxy for the striking vehicle’s travel speed prior to the crash. The current study used velocity data from event data recorders (EDRs) in rear-end crashes to evaluate this assumption. These same data were used to replicate another study (Farmer 2003) that showed the speed limit was a poor surrogate for delta-V in rear-end crashes. Method: A total of 11,199 crash records during 2017–2020 were extracted from the Crash Investigation Sampling System database. The analysis was restricted to 436 of these records that involved two vehicles with a front-to-rear manner of collision or rear-end crash configuration with EDR data from the striking vehicle. The relationships between the posted speed limit and striking-vehicle travel speed and between the speed limit and delta-V were modeled using regression. Results: On average, the speed limit overestimated striking-vehicle travel speed by 2 mph, but the relationship between the speed limit and travel speed was not linear. The speed limit reasonably approximated travel speed on roads with speed limits of 30 and 50 mph or higher. It slightly overestimated travel speed on roads with 40–45 mph speed limits and underestimated it on roads with limits of 25 mph or less. The probability that the striking vehicle’s travel speed was 25 mph or less on any road was 0.09. In contrast, the probability of the striking vehicle’s travel speed being 45 mph or less was 0.54 overall and 0.75 or higher for roads with a speed limit between 25 and 45 mph. As found in prior research, there was no significant relationship between the speed limit and delta-V. Conclusion: The posted speed limit was a reasonable surrogate for the striking vehicle’s travel speed prior to police-reported rear-end crashes on roads with a speed limit above 25 mph. It was not a reasonable surrogate for delta-V. Travel speeds on roads with speed limits of 25 mph or less were much higher than the speed limit, which suggests that existing scenarios used to evaluate FCP system performance represent travel speeds in fewer police-reported rear-end crashes than previously thought. Increasing speed differentials in existing FCP test scenarios to 45 mph would reflect the striking vehicle’s travel speed in three quarters of rear-end crashes on roads with a speed limit of 45 mph or less.
Insulin resistance by the triglyceride-glucose index in a rural Brazilian population

ABSTRACT Objective: The aim of this study was to estimate the prevalence of insulin resistance (IR) in a rural population in Brazil, to verify its association with sociodemographic, labor, lifestyle, and health factors. Subjects and methods: This is a cross-sectional study with 790 farmers in the state of Espírito Santo/Brazil. Triglyceride-glucose (TyG) was calculated and a cut-off point of Ln 4.52 was used. A hierarchical logistic regression for the association of insulin resistance with sociodemographic, labor, lifestyle and health variables of farmers living in Espírito Santo was performed. Results: The prevalence of insulin resistance was 33.7% (n = 266), and the association with insulin resistance was found in the age group 31 to 40 years of age (OR = 1.85; 95% CI 1.19-2.87); in smokers or former smokers (OR = 1.63; 95% CI 1.08-2.48) and overweight (OR = 3.06; 95% CI 2.22-4.23). Conclusion: The prevalence of insulin resistance was high in a rural population of Brazil, and was mainly associated with age, smoking and obesity. The use of TyG as an instrument for assessing the health of individuals living in areas where access to health services is difficult, such as rural areas, can represent an important advance in terms of health promotion, protection and recovery. In addition, by identifying the risk factors associated with IR, as well as their consequences, a more adequate scheme for the prevention and treatment of these comorbidities can be defined.
Association between sleep quality and occupational factors in Brazilian correctional officers

ABSTRACT Objective To analyze the occupational factors associated with poor sleep quality in correctional officers (COs). Method This is a cross-sectional study involving COs who work at four prison facilities in the state of São Paulo, Brazil. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI). Logistic and linear regression models were used to calculate the odds ratio (OR) and beta coefficient (β) of poor sleep quality and the corresponding 95% confidence interval (CI), respectively. The analyses were adjusted for the main confounders. Results Considering the 256 male COs who participated in the study, 148 (57.8%) had poor sleep quality. In the adjusted analyses, poor sleep quality was associated with higher mental demands (OR = 3.10; 95% CI: 1.40–6.85), having suffered psychological violence at work (OR = 2.13; 95% CI: 1.04–4.35), agreeing with the following statement: “I think about work-related issues in my free time” (OR = 5.13; 95% CI: 1.26–20.78), feeling partially dissatisfied (OR = 2.26; 95% CI: 1.09–4.68) or dissatisfied with work (OR = 4.52; CI: 1.32–15.46) and willingness to find another job (OR = 3.20; 95% CI: 1.53–6.71). The occupational factors dissatisfaction with work (β = 1.02; 95% CI: 0.05–1.98) and having health problems related to the work environment (β = 0.32; 95% CI: 0.24–2.41) were also associated with higher scores on the PSQI. Conclusion Poor quality of sleep is a highly frequent health problem among correctional officers, and this condition is associated with the perception of poor working conditions, professional dissatisfaction and the fact that such professionals think about work during their free time.
Complete chloroplast genomes and comparative analyses of Hippeastrum ‘milady’, Hippeastrum albertii and Hippeastrum reticulatum (Amaryllidaceae)

Hippeastrum is a genus of ornamental plants with large, brightly colored flowers. Due to the very high seed-setting rate of the hybridization of Hippeastrum, the large population of hybrid progeny and the existence of superparent inheritance, it is difficult to trace the origin of the varieties collected from the market during breeding. In this study, we analyzed the chloroplast genomes of Hippeastrum ‘Milady’, H. alberti, and H. reticulatum using the Illumina NovaSeq sequencing platform and generated full-length sequences of 158,067, 158,067, and 158,522 bp, respectively. All three genomes had the typical tetrad structure. The large single copy, small single copy, and inverted repeat regions of H. reticulatum were observed to be respectively 277, 138, and 20 bp longer than the corresponding regions of H. ‘Milady’ and H. alberti. The results of comparative analysis of simple sequence repeats (SSRs), Ka/Ks ratios, codon preferences, and complete sequences of chloroplasts of these three taxa and 14 other plant species were as follows. First, the chloroplast genomes of H. ‘Milady’, H. alberti, and H. reticulatum contain 209, 209, and 211 SSR sites, respectively, most of which (123, 123, and 122, respectively) are single nucleotide repeats. Second, leucine, arginine, and serine are the most frequently used amino acids in the three chloroplast genomes. Third, H. ‘Milady’, H. alberti, and H. reticulatum are more closely related to Lycoris and Narcissus than to Allium and Agapanthus. Our results will provide information on the study of origins or relatedness of native species, and the identification of cultivars.
Continuity and change in loneliness and stress during the COVID‐19 pandemic: A longitudinal study of autistic and non‐autistic adults

Previous studies have suggested that autistic adults may be negatively affected by the COVID‐19 pandemic and its associated restrictions. In this study, we examined continuity and change in loneliness and stress, and their predictors, in 448 autistic and 70 non‐autistic adults living in the Netherlands. Autistic participants were assessed on three occasions using the de Jong Gierveld Loneliness Scale and Perceived Stress Scale (pre‐lockdown (T0), first lockdown (T1), and second lockdown (T2)); non‐autistic participants were assessed twice (T1 and T2). Autistic adults' loneliness and stress levels remained stable across all three time points over 8 months, but were consistently higher than those of non‐autistic adults. Other predictors of higher loneliness and stress levels at the first lockdown (T1) included low perceived social support and high levels of COVID‐19 related worries. Although loneliness and stress were stable at the group level, the wellbeing of some autistic adults worsened over the course of the pandemic, while others improved. For instance, adults with a mental health diagnosis (other than autism) prior to the pandemic were more likely to increase in stress over time, whereas adults with higher perceived social support were more likely to decrease in stress over time (from T1 to T2). Factors contributing to variability in outcome require further examination. Moreover, the relatively high loneliness and stress levels in autistic adults call for attention from clinicians and service providers.
HIV prevention metrics: lessons to be learned from contraception

As the range of effective HIV prevention options, including multiple biomedical tools, increases, there are many challenges to measuring HIV prevention efforts. In part, there is the challenge of varying prevention needs, between individuals as well as within individuals over time. The field of contraception faces many similar challenges, such as the range of prevention methods and changing contraceptive needs, and has developed many metrics for assessing contraceptive use at the program level, using frameworks that move beyond the HIV prevention cascade. We explore these similarities and differences between these two prevention fields and then discuss how each of these contraceptive metrics could be adapted to assessing HIV prevention.
Porous amorphous nitinol synthesized by argon injection: a molecular dynamics study

Porous crystalline nitinol is widely applied in various fields of science and technology due to the unique combination of physical and mechanical properties as well as biocompatibility. Porous amorphous nitinol is characterized by improved mechanical properties compared to its crystalline analogues. Moreover, this material is more promising from the point of view of fundamental study and practical application. The production of porous amorphous nitinol is a difficult task requiring rapid cooling protocol and optimal conditions to form a stable porous structure. In the present work, based on the results of molecular dynamics simulations, we show that porous nitinol with the amorphous matrix can be obtained by injection of argon into a liquid melt followed by rapid cooling of the resulting mixture. We find that the porosity of the system increases exponentially with increasing fraction of injected argon. It has been established that the system should contain about ∼18% –23% argon for obtain an open porous structure, while the system is destroyed by overheated inert gas when the argon fraction is more than ∼23 %. It is shown that the method of argon injection makes it possible to obtain a highly porous system with the porosity ∼70 % consisting the spongy porous structure similar to aerogels and metallic foams.
MURPHREE FESTSCHRIFT: RESEARCH REPORT Identification of novel genes by targeted exome sequencing in Retinoblastoma

Background: Retinoblastoma (RB) is initiated by mutation in both alleles of RB1 gene. However, few cases may occur even in the absence of RB1 mutation suggesting the role of genes other than RB1. Methodology: The current study was planned to utilize targeted exome sequencing in Indian RB patients affected with unilateral non-familial RB. 75 unilateral RB patients below 5 years of age were enrolled. Genomic DNA was extracted from blood and tumor tissue. From peripheral blood DNA, all coding and exon/intron regions were amplified using PCR and direct sequencing. Cases which did not harbor pathogenic variants in peripheral blood DNA were further screened for mutations in their tumor tissue DNA using targeted exome sequencing. Three pathogenicity prediction tools (Mutation Taster, SIFT, and PolyPhen-2) were used to determine the pathogenicity of non-synonymous variations. An in-house bioinformatics pipeline was devised for the mutation screening by targeted exome sequencing. Protein modeling studies were also done to predict the effect of the mutations on the protein structure and function. Results: Using the mentioned approach, we found two novel variants (g.69673_69674insT and g.48373314C>A) in RB1 gene in peripheral blood DNA. We also found novel variants in eight genes (RB1, ACAD11, GPR151, KCNA1, OTOR, SOX30, ARL11, and MYCT1) that may be associated with RB pathogenesis. Conclusion: The present study expands our current knowledge regarding the genomic landscape of RB and also highlights the importance of NGS technologies to detect genes and novel variants that may play an important role in cancer initiation, progression, and prognosis. ARTICLE HISTORY Received 24 February 2022
Basis and current state of computed tomography perfusion imaging: a review

Computed tomography perfusion (CTP) is a functional imaging that allows for providing capillary-level hemodynamics information of the desired tissue in clinics. In this paper, we aim to offer insight into CTP imaging which covers the basics and current state of CTP imaging, then summarize the technical applications in the CTP imaging as well as the future technological potential. At first, we focus on the fundamentals of CTP imaging including systematically summarized CTP image acquisition and hemodynamic parameter map estimation techniques. A short assessment is presented to outline the clinical applications with CTP imaging, and then a review of radiation dose effect of the CTP imaging on the different applications is presented. We present a categorized methodology review on known and potential solvable challenges of radiation dose reduction in CTP imaging. To evaluate the quality of CTP images, we list various standardized performance metrics. Moreover, we present a review on the determination of infarct and penumbra. Finally, we reveal the popularity and future trend of CTP imaging.
Microsatellite instability and oncological outcomes in Thai patients with endometrial cancer

Abstract Here, we determined the frequency of microsatellite instability (MSI) and the impact of MSI-high (MSI-H) on clinical outcomes of Thai patients with endometrial cancer (EC). Tissue samples of 110 Thai patients with EC, who had undergone surgical staging, were tested for mismatch repair (MMR) gene deficiency, and the patients were grouped into MSI-H and MSI-stable (MSI-S) groups; 24.5% had MSI-H. Unlike MSI-S group patients, MSI-H group patients had synchronous and metachronous cancer. They showed better 3-year disease-free survival (DFS) than those in the MSI-S group (p=.182; 92.3% vs. 82.6%). The 3-year overall survival was 96.2% in MSI-H and 86.4% in MSI-S groups (p=.163). Multivariate analyses showed lower uterine involvement (p=.004), myometrial invasion ≥50% (p=.032), lymphovascular space invasion (p<.001) and MSI-S (p=.006) as prognostic factors for DFS. Our study showed that the prevalence of MMR gene deficiency in Thai patients with EC is common and associated with better outcomes. Impact Statement What is already known on this subject? Microsatellite instability (MSI) occurs in approximately 20–40% of endometrial cancer (EC) cases. MSI analysis in EC can identify patients at higher risk of hereditary nonpolyposis colorectal cancer and those having prognostic factors. Additionally, it is predictive of immune checkpoint inhibitor treatment. However, current evidence shows a correlation between clinicopathological characteristics and EC prognosis. Studies on EC and MSI status effect on survival outcome have yielded inconsistent results regarding the pathological significance of MSI in such malignancies. What do the results of this study add? The prevalence of mismatch repair (MMR) gene deficiency in Thai patients with EC is common (24.5%) and associated with better outcomes. What are the implications of these findings for clinical practice and/or further research? This study highlights the prevalence and impact of MSI on oncological outcomes in patients with EC in a low-incidence country. Future studies should focus on the detection of germline mutation to understand the accurate prevalence of Lynch syndrome in Thai patients with EC.
Exploring the Antibacterial Properties of Lignin‐coated Magnetic Nanoparticles Synthesized in a One‐pot Process †

Given the current grave problems with antibiotic resistance, the discovery of novel, unconventional antibacterial drugs is not just important but also urgent. In this contribution, we report on the synthesis and testing of several composite nanomaterials that may find applications as therapeutic drugs or surface disinfectants. These materials are based on magnetic nanostructures coated with lignin, for example, lignin@FeCo. The magnetic properties of these nanocomposites facilitate removal or localization, whereas the lignin shell provides biocompatibility. These nanomaterials are mild antibacterials in the absence of light, but when illuminated become powerful antibacterial agents with typically ≥6 log units of bacterial reduction in 1–5 min of irradiation. These materials are strongly absorbing, including in the very useful NIR biological window, which we illustrate using 810 nm LED irradiation. We also show that in the short time required for antibacterial action, thermal changes are very small (≤5°C). Further, biocompatibility tests using fibroblasts show very limited cell damage and no enhanced adverse effect during 810 nm NIR illumination. As a surface coating for the active material, lignin provides a “trojan horse” strategy to facilitate the antibacterial action.
The nature of waves in the arteries in memoriam: Nico Westerhof and John Tyberg

This memorial remembers the lives of two distinguished researchers who made major contributions to cardiovascular physiology; Nico Westerhof (1937–2022) and John Tyberg (1938–2022). It is a joint memorial not because they collaborated closely but because they held very different views about the nature of waves in the arteries. Their papers and particularly their lively discussions at many scientific meetings stimulated interest in the subject. Both were thoughtful and articulate about their views, and the debates were polite and dignified. They never resolved their differences and, after outlining what these differences were, we will suggest that perhaps there is no resolution. The authors of this memorial were close to one or the other protagonist; a son, a son‐in‐law and two close collaborators. We all have different views about the nature of waves in the arteries, but we all share great respect for both men and felt that a joint memorial was a fitting way to remember them and their many contributions. All of the authors knew the subjects of this memorial as ‘Nico’ and ‘John’, and we will use these informal names throughout.
Trustworthiness in Qualitative Research

To be relevant, all research must be trustworthy. Although quips about statistics are often cynical—as numbers can be manipulated by those with an agenda—at least quantitative research has the facade of being intrinsically trustworthy. This is because the quantitative research process is more systematic and apparent, and finding relationships between numbers is assumed to be more rigorous than deriving meaning from words and actions. Qualitative research, for reasons that will be laid out in this article, is considered by many in the scientific community to be less credible. This perception, whether real or imagined, makes trustworthiness a compelling goal. In this article, I will explore what it means for a qualitative study to be trustworthy, how trustworthiness can be attained by researchers, and how lactation professionals can assess the studies they read for trustworthiness.
Patient-Reported Outcome of Functional Cognition for Traumatic Brain Injury: Item-Level Psychometrics

A patient-reported functional cognition measure that can bridge neuropsychological tests and ecological validity is lacking. The purpose of this study was to present the initial item-level psychometrics of a functional cognition item bank, the Functional Cognition–Patient-Reported Outcome (FC-PRO). Confirmatory factor analysis, Rasch analysis, and convergent validity were conducted to establish item-level psychometrics of the FC-PRO item bank. Four of the six domains met essential unidimensionality criteria; all domains met rating scale criteria; domains had relatively few misfitting items (3%–13%); person reliability (.8–.92), and person strata (2.97–4.29) were satisfactory; the domains showed convergent validity for five of six domains. The FC-PRO showed adequate initial item-level psychometrics for researchers/clinicians to measure specific functional cognitive domains in patients with traumatic brain injury (TBI). This study provides the foundation for the development of future short forms, computer adaptive tests, and keyform recovery maps of patient-reported functional cognition to facilitate clinical utilization.
Activation of the JAK1/STAT1 signaling pathway is associated with peroxiredoxin 6 expression levels in human epididymis epithelial cells.

INTRODUCTION Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis, present in human seminal fluid, and in spermatozoa. The protective role of Prdx6 in maintaining the viability and DNA integrity of human spermatozoa was also detected. Here, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs). MATERIAL AND METHODS Western blotting was used to measure expression levels of key proteins in the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. The malonaldehyde (MDA) levels and antioxidant capacity in HEECs were detected with the commercial kits. Digital gene expression analysis (DGE) was used to identify gene expression patterns in control and Prdx6-interference HEECs. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to validate the DGE findings. RESULTS Compared to control HEECs, the expression levels of JAK1, STAT1, phosphorylated JAK1 and STAT1 were significantly increased, while the expression level of SOCS3 was significantly decreased in Prdx6-interference HEECs. The MDA level and total antioxidant capacity in Prdx6-interference HEECs were significantly increased and decreased compared to that of control, respectively. DGE analysis identified 589 up-regulated and 314 down-regulated genes (including Prdx6) in Prdx6-interference HEECs. Thirteen significantly different pathways were identified between the two groups, with the majority of genes belonging to the CCL, CXCL, IL, and IFIT family of proteins and were related to immunity. In particular, the expression levels of IL6, IL6ST, and eighteen IFN-related genes were significantly increased in Prdx6-interference HEECs compared to control HEECs. CONCLUSIONS We found that reduced Prdx6 expression induced higher ROS levels in HEECs, which resulted in the activation of the IL-6 receptor and IFNγ expression to induce the JAK1/STAT1 signaling pathway.