Miracle-dz/UniFAR-FTUs huggingface dataset:
Étude cellulaire et moléculaire de la germination chez Medicago truncatula

La germination est un processus en continuite avec la fecondation, l'embryogenese et la maturation de la graine. Il debute avec l'imbibition mais est soumis a des controles internes et externes : balance hormonale qui regule les acteurs de la germination et conditions environnementales. Ce travail apporte des informations sur la germination des semences de Medicago truncatula. Chez cette espece, la germination resulte uniquement de la croissance cellulaire par elongation. La restructuration des parois joue un role cle et serait la cible de l'acide abscissique (ABA). Elle correspond a la modification de la composition en oses et a l'evolution des structures internes a l'echelle des polymeres ; notamment, l'apparition de callose et des changements au niveau des ramifications des pectines. Les parois sont riches en arabinose, presentent une heterogeneite tissulaire, et delimitent des structures en fuseaux dans l'axe embryonnaire. Le gene MtSAP1 a ete etudie au niveau structural et fonctionnel. Il code pour une proteine possedant les domaines A20 et AN1. Chez les mammiferes ses homologues regulent les processus inflammatoire et la mort cellulaire. Chez le riz, les proteines A20/AN1 joueraient un role dans la transmission du signal de stress. Le travail effectue ici montre que MtSAP1 s'exprime dans l'axe embryonnaire dans la semence en fin de maturation. Son expression chute durant la germination et il est fortement induit par l'ABA et par l'hypoxie. Des interactions existent entre MtSAP1 et des proteines liees au developpement et aux stress. Son role pourrait s'exercer au niveau de la transmission du signal stress lie a la dessiccation et le faible taux d'oxygene.
New Zealand and south-east Australian ilyarachninae (isopoda: asellota: munnopsidae) and their worldwide relationships

The diversity of New Zealand and Australian asellote isopods remains largely unknown. Even less is known about how this fauna is related to the rest of the world. This study focuses on the munnopsid subfamily Ilyarachninae Hansen, 1916, to explore the phylogenetic and biogeographic relationships of the local fauna to others from this group worldwide. To analyse the relationships within the Ilyarachninae, a suitable outgroup, in this case, the sister group, was needed to be determined. To do this, a cladistic analysis of the Munnopsidae was undertaken. In addition to determining a suitable outgroup, the family level analysis also served to test the monphyly of the nine subfamilies and to resolve the placement of the three genera, Munneurycope Stephensen, 1913, Munnicope Menzies and George, 1972 and Munnopsurus Richardson, 1912b which were not placed in any exsiting subfamily. The cladistic analysis of the Munnopsidae included 72 species representing all munnopsid genera, including where possible, the type species and was based on 122 morphological characters. A well resolved strict consensus tree was found. The results show Munnopsurus to be the sister group of the Ilyarachninae and the Munnopsinae to be the most ancestral group of the Munnopsidae. Of all the subfamilies, the Betamorphinae is not a monophyletic. The results show that although some subfamily names are upheld, others show no congruence to the topology of the results, and therefore the use of subfamily names are abandoned. Four families are instead recognized, the Munnopsidae (restricted to the former Munnopsinae), Acanthocopidae Wolff, 1962, the Syneurycopidae Wolff, 1962 and the Lipomeridae Tattersall, 1905a, with the latter including the former Ilyarachninae (‘Ilyarachna-group’). Within the Ilyarachna-group, two new genera and 28 species were described, including 25 species new to science. Two genera, Notopais and Epikopais n. gen. are found exclusively in the southern hemisphere. Of the more cosmopolitan genera, some New Zealand and south-east Australian species display close affinities to each other and with Antarctic species. These relationships were tested using cladistic phylogenetic methods, and in addition to
Development of Novel Tools for Radial Glia Lineage Tracing and Modeling Central Nervous System Tumor

Methods that combine lineage tracing with cellular transgenesis are needed in order to determine mechanisms that specify neural cell types. Currently available methods include viral infection and Cre-mediated recombination. In utero electroporation (IUE) has been used in multiple species to deliver multiple transgenes simultaneously into neural progenitors. In standard IUE, most plasmids remain episomal, are lost during cell division, and so transgenes are not expressed in the complete neural lineage. Here we combined IUE with a binary piggyBac transposon system (PB-IUE), and show that unlike conventional IUE, a single embryonic transfection of neocortical radial glia with a piggyBac transposon system results in stable transgene expression in the neural lineage of radial glia: cortical neurons, astrocytes, oligodendrocytes, and olfactory bulb interneurons. We also developed a modular toolkit of donor and helper plasmids with different promoters that allows for shRNA, bicistronic expression, and trangenesis in subsets of progenitors. As a demonstration of the utility of the toolkit we show that transgenesis of epidermal growth factor receptor (EGFR) expands the number of astrocytes and oligodendrocyrtes generated from progenitors. The relative ease of implementation and experimental flexibility should make the piggyBac IUE method a valuable new tool for tracking and manipulating neural lineages.
Effect of TRX Suspension Training on Abdominal Strength and Isometric Muscle Endurance of Police College Students

This study discussed the effects of 12-week TRX suspension training and instrument resistance training on abdominal muscle strength and isometric abdominal muscle endurance of police college students. Thirty male students from Nanjing Forest Police College were randomly divided into TRX suspension training group and instrument resistance training group. Each group received a 90-minute training course twice a week for 12 weeks. The abdominal muscle strength and abdominal muscle tolerance were measured before and after training. After statistical analysis of T test of dependent samples, it was found that TRX suspension training group had significant improvement in abdominal muscle strength and other longissimus muscle strength, while instrument resistance training group had significant improvement only in abdominal muscle strength. Therefore, the choice of TRX suspension training, compared with the device resistance training, can significantly enhance the core muscle capacity. Keywords—Police college; TRX; Abdominal strength; Isometric muscle endurance
Evolutionary relationships in the Spermacoceae alliance (Rubiaceae) using information from six molecular loci: insights into systematic affinities of Neohymenopogon and Mouretia

Several recent phylogenetic studies of Rubiaceae have dealt with enigmatic taxa whose systematic positions have been previously unknown or controversial. We address evolutionary relationships in the Spermacoceae alliance (Rubioideae) with special emphasis on the Asian genera Mouretia and Neohymenopogon, here sequenced for the first time. Both genera belong in the tribe Argostemmateae and have persistent calyx lobes on the fruit in common with Argostemma and Mycetia. Other previous uncertainties are resolved with strong support; Saprosma is sister to Paederieae s. str. and Carpacoce is sister to remaining Anthospermeae. Our results further reveal some phylogenetic problems. Danaideae is sister to remaining taxa in the Spermacoceae alliance with high posterior probability, which contradicts results in a recent study. The uncertainty concerning evolutionary relationships of Dunnia and Theligonum is reinforced, despite a denser taxon sampling in the Spermacoceae alliance compared with earlier studies. We also demonstrate yet another example of the controversial correlation between molecular substitution rate and plant life history.
Effects of BSE on depression/anxiety in women diagnosed with breast cancer

This study employed three questionnaires to determine if depression, anxiety and mood levels in women recently diagnosed with breast cancer differed when the breast lump was discovered via breast self-exam versus discovery by physician examination, mammogram or accidental discovery by self or spouse. Depression was measured by the Beck Depression Inventory, anxiety was measured by the Spielberger State-Trait Anxiety Scale and mood was measured by the General Health Questionnaire. The Neuman Systems Model was the conceptual framework for this study. Sixty-two women between the ages of 26-80 years completed the three questionnaires while awaiting the physician during an office visit. All women completed the questionnaires within 12 weeks of diagnosis. Data was analyzed utilizing Analysis of Variance and Analysis of Covariance. Significant differences in depression, mood and anxiety were not found to exist between women who discovered the lump through BSE and women diagnosed as a result of physician examination, mammogram or accidental discovery. Depression and anxiety scores were statistically significant when groups were separated into BSE practice versus no BSE practice regardless of the method of discovery. Women who practiced BSE were less depressed and less anxious overall than women who did not practice BSE.
Analysis of limb movement synchronization in primates locomotion

Abstract The authors present an original mathematical model based on features identified with discrete variables using vector and hierarchical cluster analysis in primates locomotion. Proposed model allows to formalize and analyze the synchronization variability of movements in given locomotion types of adaptation and specialization in monkeys, apes and humans. The material covers observations of 102 forms including 9 species of primates: the chimpanzee, bonobo, orangutan, gibbon, gelada, mandrill, brown capuchin and ring–tailed lemur. The studies included also the synchronization of locomotory movements in man. The sequences of moves of pectoral and pelvic limbs, right and left, were studied in four categories: walking, running, jumping and brachiation. The locomotion movements depend on the brain centers and allow to find phylogenetic relations between examined forms in the evolution process. The knowledge of the pattern of movements is used in the treatment of paraplegia and paraparesis in humans.
Kronik Hastalıklarda Hastalık Algısı, Yaşam Kalitesi, Öz-Bakım Yönetimi

Uzun sureli ve yavas ilerleyen kronik hastaliklar saglik hizmetlerinde yukselen bir sorundur ve bir veya daha fazla kronik hastaligi olan bireylerin sayisi her gecen gun artmaktadir. Bu kesitsel calisma, kronik hastaligi olan bireylerde hastalik algisi, oz bakim yonetimi ve yasam kalitesi arasindaki iliskiyi saptamak amaciyla yapildi. Calismanin orneklemini ic hastaliklari ve cerrahi kliniklerinde yatan, en az bir kronik hastaligi olan ve calismaya katilmayi kabul eden 127 hasta olusturdu. Veriler kisisel bilgi formu, hastalik algisi anketi (IPQ), kronik hastaliklarda oz bakim yonetimi olcegi (SCMP-G Anketi) ve yasam kalitesi anketinin EQ-5D-5L genel kalitesi ile toplanmistir. Istatistiksel analiz sonucunda, oz bakim yonetimi olceginin kendi kendini koruyan alt olcegi puanlarinin kadinlarda erkeklerden anlamli duzeyde daha yuksek oldugu gorulmustur. Solunum sistemi hastaligi olan hastalarin oz bakim yonetimi, diyabetik hastalardan anlamli derecede yuksekti. Bu calismanin sonucunda, kadin hastalarin erkek hastalara gore daha iyi oz bakim yonetimine sahip olduklari gorulmustur. Ancak, erkeklerin kadinlardan daha iyi bir yasam kalitesine sahip olduklari bulunmustur. Solunum hastaligi olan hastalar, diyabetik hastalardan daha iyi bir oz bakim yonetimine sahipti.
The Effect of Lentils on Postprandial Glycemic Response in Healthy Adults and the Ability of In Vitro Digestion to Predict Glycemic Response

THE EFFECT OF LENTILS ON POSTPRANDIAL GLYCEMIC RESPONSE IN HEALTHY ADULTS AND THE ABILITY OF IN VITRO DIGESTION TO PREDICT GLYCEMIC RESPONSE Dita Moravek Advisors: University of Guelph Dr. Alison M. Duncan Dr. Dan D. Ramdath Diets rich in carbohydrates that have a low glycemic index (GI) are inversely associated with type 2 diabetes (T2D) risk (Ley et al. 2016) due to the favourable postprandial glycemic response (PPGR). Whether substituting pulses, such as lentils, for a portion of a different starchy foods improves PPGR is unknown. The objectives of this research were: 1) to determine the effects on PPGR of consuming rice or potato alone or in combination with lentils, 2) to determine the effects on PPGR of consuming lentil-based food products compared to the same (control) foods prepared with high-GI starches, and 3) to relate the composition of lentil starch to in vivo PPGR and in vitro digestion variables. For objective #1, healthy adults completed a randomized, crossover clinical trial in which acute glycemic response was examined following consumption of rice (n=24) or potato (n=24) alone or in combination with one of three lentil varieties (large green, small green or split red). Blood glucose incremental area under the curve (iAUC) and maximum concentration (CMAX) were significantly lower following the small green and split red lentil treatments compared to the rice control and for all lentil treatments compared to potato control. For objective #2, healthy adults completed a randomized, crossover clinical trial in which acute glycemic response was examined following consumption of food products (muffin, n=24; chili, n=24; soup, n=20) that contained one of two different lentil varieties (small green or split red) or a commonly consumed starch source (wheat, rice or potato). Blood glucose iAUC and CMAX were significantly lower following lentil-containing chilies and soups compared to controls, while only the blood glucose iAUC for red lentil muffins was significantly lower compared to the control. Finally, the data from these trials was used in combination with in vitro data to explore relationships and discover if in vitro measurements could predict in vivo PPGR. Although more methodological consistency was required to produce reliable relationships, in vitro measurements were strongly correlated with in vivo glycemic and insulinemic responses. These results support the consumption of lentils to attenuate postprandial glycemia.
Social Networks and Fitness Consequences of Early Sociality in Wild Bottlenose Dolphins (Tursiops sp.)

Early social development in long-lived social mammals has important implications for adult behavior, particularly in taxa that exhibit stable long-term bonds. In one such species, bottlenose dolphins, calves have precocious locomotion, enabling them to separate from their mothers soon after birth and associate with other dolphins in the absence of direct maternal influence. To investigate mother and calf social patterns while together and separated, we analysed the ego networks of 27 mother–calf pairs constructed using group composition data from focal follows and evaluated differences based on calf sex. When separated, all calves had larger, less dense ego networks than their mothers, whereas ego networks of mother and calf when together were similar in size to those of calves, but significantly less dense. Most intriguingly, during separations, male calves’ relationships with other male calves were stronger than expected, foreshadowing the long-term bonds between adult male alliance partners. Female–female calf relationships were not stronger than expected, but when together, mothers and female calves had strong relationships with juvenile females. These results support the social bonds hypothesis and suggest that temporary separations allow calves to build and strengthen their social networks. Since bottlenose dolphins show bisexual philopatry and infant * A version of this chapter appears as: Stanton, M.A., Gibson, Q.A. , & Mann, J. 2011. Animal Behavior, 82, 405-412. 104 relationships can persist into adulthood, calf independence and early social development probably have implications for future success.
Effects of policosanol on older hypercholesterolemic patients consumed calcium channel blockers

El policosanol es un medicamento reductor del colesterol con efectos anti-plaquetarios concomitantes cuya eficacia, seguridad y tolerabilidad se documentan en estudios clinicos y de post-comercializacion. El objetivo de este estudio fue investigar si el policosanol modifica los efectos antihipertensivos de los bloqueadores del canal de calcio y/o si dicha combinacion dana algun indicador de seguridad o induce algunos eventos adversos especificos en ancianos. Para este estudio se analizaron los registros de todos los pacientes (313) que tomaban bloqueadores de canales de calcio incluidos en un estudio de prevencion en ancianos en el que 1470 pacientes con alto riesgo coronario fueron aleatorizados y recibieron policosanol 5 mg/d o placebo durante 3 anos. El analisis fue por intencion de tratar. Las caracteristicas basales fueron similares en ambos grupos. La eficacia del policosanol para reducir el colesterol fue evidente y persistente. El policosanol no afecto los indicadores de seguridad investigados. La frecuencia de eventos adversos severos en pacientes tratados con policosanol (4/155; 2,6%) fue menor que en el grupo placebo (24/158; 15,2%), asi como la frecuencia de eventos adversos leves o moderados (36/155, 23,2%) tambien fue menor que en el placebo (52/158; 32,9%). Se concluye que el policosanol fue bien tolerado, sin aumentar ningun evento adverso en comparacion con el placebo. Estos resultados confirman que el consumo de policosanol por ancianos hipercolesterolemicos que tambien toman bloqueadores de los canales de calcio lejos de revelar cualquier interaccion medicamentosa adversa, fue beneficioso para los pacientes del estudio.
Sarcopenic obesity negatively affects muscle strength, physical function and quality of life in obese elderly women

We evaluated the negative effect of sarcopenic obesity (SO) on muscle strength, physical function and quality of life in obese elderly women using the appendicular lean mass (aLM) adjusted for BMI, and aLM adjusted for height and fat mass residuals approach. Participated fifty-eight obese elderly women aged 60 to 70 years separated by two groups (OS) and non-sarcopenic (OS) in two different approaches. The prevalence of SO was also verified between methods and its effects on handgrip strength, lean body mass, chair-stand test, time-up-and-go test, six-minute-walk test, peak O 2 consumption, heart rate recovery and quality of life. The SO group presented significantly lower lean body mass, lower handgrip strength, inferior scores in the functional tests, inferior aerobic fitness, an impaired heart rate recovery, and an inferior aspect of quality of life as compared with the NSO group. Furthermore, no elderly woman was classified with SO by the aLM adjusted for height and fat mass residuals method. The cutoff-point addressed by the aLM/BMI represents a tool in clinical geriatric practice to identify and prevent this obesity/muscle syndrome in elderly women.
Determination of the Frequency of blaCTX-M, blaSHV and blaTEM Genes in Escherichia Coli Isolated from Burns Patients in Tehran Shahid Motahari Hospital

The genes blaSHV, blaTEM, blaCTX-M of the betalactamase enzymes are located on transportable elements. The aim of this study was to determine the abundance of Escherichia coli producing ESBL, lactamase genes of blaSHV, blaTEM, blaCTX-M by Multiplex PCR and their relationships by creating antibiotic resistance in strains of E. Coli. Clinical samples were collected from burns patients hospitalized at Motahari hospital of Tehran in the last quarter of 2015. Bacteria were identified by standard biochemical methods. Antibiotic sensitivity of E. Coli against common antibiotics in the pharmaceutical market of Iran,was determind by Kirby – Bauer method. DNA was then isolated from the bacteria and presence of antibiotic resistant genes was determined by PCR and multiplex PCR. Antibiotic resistance and presence of blaTEM, blaSHV, blaCTX-M genes were determined in 100 clinical strains of Escherichia coli and standard strain ATCC 25922. Findings: In total, 167 urine samples were investigated and after biochemical testing 100 E. Coli were isolated from the samples. Antibiotic suscep tibility test results indicated that the highest and the lowest resistances were related to Ampicillin (97%) and Nitrofurantoin (11%), Imipenem (0%) respectively. It means none of the isolates was found to be resistant to Imipenem. 63 samples of ESBL were positive, analysis of extension of blaSHV, blaTEM and blaCTX-M genes were 44.4%, 61.90% and 71.42% respectively. The results indicate that the process of antibiotic resistance is on the rise. Development of strains resistant to several antibiotics (MDR) is a significant health threat. Resistance patterns in each region can vary; therefore, accurate identification of resistant strains of each region, providing antibiogram, education of laboratory staff on the correct interpretation of the results of antibiogram and providing detailed reports to physicians can reduce mortality, reduce treatment costs, prevent failures and reduce the spread of resistance.
Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-a gene with rheumatoid arthritis

Introduction Multifunctional pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in a variety of inflammatory diseases including rheumatoid arthritis (RA). TNF-α polymorphisms are mostly located in its promoter region and play a significant role in disease susceptibility and severity. We therefore sought to investigate TNFA –863C/A (rs1800630) polymorphism association with RA activity in our Pakistani study group. Material and methods A total of 268 human subjects were enrolled. Among them, 134 were RA patients and 134 were controls. In this study the physical parameters of RA patients were collected, and the disease activity was measured by DAS28. The genotypes were determined following the allele-specific PCR along with the pre-requisite internal amplification controls. Subsequently, data were analyzed statistically for any significant association including χ2/Fisher’s exact test using GraphPad prism 6 software. Results We found that the TNF-α –863 C/A (rs1800630) variant was not differentially segregated between cases and controls in either genotype frequency, with χ2 of 2.771 and a p-value of 0.2502, or allele frequency, with χ2 of 2.741 and a p-value of 0.0978, with an odds ratio (95% CI) of 0.7490 (0.5317–1.055). Conclusions The lack of positive association of TNF-α –863(rs1800630) polymorphism in our study group implies that TNF-α –863 polymorphism is not a susceptible marker to RA and cannot serve as a genetic factor for screening RA patients in Pakistan. There might be other factors that may influence disease susceptibility. However, further investigations on additional larger and multi-regional population samples are required to determine the consequences of genetic variations for disease prognosis.
Functional recombinant antibodies against human chorionic gonadotropin expressed in plants

Single-chain variable fragments, diabodies and chimeric antibodies (mouse variable domains and human immunoglobulin constant domains) were engineered by DNA recombinant technique and expressed transiently in tobacco leaves. The plants expressed the three types of antigen-binding moieties, accurately and faithfully. The yield obtained was 32 mg, 40 mg and 20 mg respectively, per kg of wet weight of leaves. The chimeric antibody had high affinity for human chorionic gonadotropin (Ka = 1.9 x 10 1 0 M - 1 ). All three forms of the recombinant antibodies expressed by plants inhibited the binding of hCG to receptor on Leydig cells.