Miracle-dz/UniFAR-FTUs huggingface dataset:
TIGIT/PVR and LncRNA ANRIL dual-targetable PAMAM polymeric nanoparticles efficiently inhibited the hepatoma carcinoma by combination of immunotherapy and gene therapy

Abstract Herein, a novel polymeric nanoparticle was designed to inhibit hepatoma carcinoma by simultaneously targeting the T cell immunoreceptor with Ig and ITIM domains (TIGIT)/poliovirus receptor (PVR) and long noncoding RNAs antisense noncoding RNA in the INK4 locus (LncRNA ANRIL). Firstly, the siANRIL-loaded nanoparticles (NP-siANRIL) was developed by methoxy-poly (ethylene glycol)-polyamidoamine (mPEG-PAMAM) and polyamidoamine-poly (ethylene glycol)-disulphide bond-carboxyl (PAMAM-PEG-S2-COOH) using the self-assembly method. Then the DTBP-3 peptide, a newly developed identified peptide which could occupy the binding interface and effectively block the interaction of TIGIT with its ligand PVR, was further conjugated on the surface of NP-siANRIL via the glutathione (GSH)-sensitive disulphide linkage. In this way, the binding ability of DTBP-3 to TIGIT was remained once they were entrapped into the tumour tissues which were abundant of GSH. The present study demonstrated that DTBP-3NP-siANRIL exhibited an excellent anti-tumour effect on hepatoma carcinoma in vivo by simultaneously inhibited the expression of miR-203a and its downstream genes and increased the percentages of NK cells and T cells. In a word, the present study has presented a novel strategy for treatment of hepatoma carcinoma by simultaneously targeting of TIGIT/PVR and LncRNA ANRIL.
Effectiveness and cost‐effectiveness of the Transmural Trauma Care Model investigated in a multicenter trial with a controlled before‐and‐after design: A study protocol

Abstract Objective The rehabilitation of trauma patients in primary care is challenging, and there are no guidelines for optimal treatment. Also, the organization of care is not well‐structured. The Transmural Trauma Care Model (TTCM) has been developed in the Netherlands, aiming to improve patient outcomes by optimizing the organization and the quality of the rehabilitation process in primary care. A recent feasibility study showed that implementation of the TTCM at a Dutch Level 1 trauma center was feasible, patient outcomes were improved, and costs were reduced. This study aims to assess the effectiveness and cost‐effectiveness of the TTCM compared to the usual care in a multicenter trial. Methods A multicenter trial with a controlled before‐and‐after design will be performed at 10 hospitals in the Netherlands. First, participating hospitals will include 322 patients in the control group, receiving usual care as provided in these specific hospitals. Subsequently, the TTCM will be implemented in all participating hospitals, and hospitals will include an additional 322 patients in the intervention group. The TTCM consists of a multidisciplinary team at the outpatient clinic (trauma surgeon and hospital‐based physical therapist), an educated and trained network of primary care trauma physical therapists, and structural communication between them. Co‐primary outcomes will investigate generic and disease‐specific, health‐related quality of life. Secondary outcomes will include pain, patient satisfaction, perceived recovery, and patient‐reported physical functioning. For the economic evaluation, societal and healthcare costs will be measured. Measurements will take place at baseline and after 6 weeks, 3, 6, and 9 months. Analyses will be based on the intention‐to‐treat principle. Missing data will be handled using longitudinal data analyses in the effect analyses and by multivariate imputation in the economic evaluation. Conclusion This trial with a controlled before‐and‐after design will give insight into the effectiveness and cost‐effectiveness of the TTCM in a multicenter trial.
Creatinine and NT-ProBNP levels could predict the length of hospital stay of patients with decompensated heart failure

Abstract Background Heart failure (HF) is a clinical syndrome that causes high morbidity and mortality with a high number of admissions and sometimes prolonged admissions. This study aimed at assessing whether parameters detected during the first 24 h of admission may predict a prolonged hospital stay in patients admitted to hospital for decompensated HF. Methods From January 2016 to December 2019, 2359 admissions of decompensated HF were recorded. In-hospital transfers, de novo HF, deaths and scheduled admissions were discarded to homogenise the sample. Finally, 1196 patients were included. The sample was divided into two groups: (a) non-prolonged admission (n = 643, admission ≤7 days) or (b) prolonged admission (n = 553, admission >7 days). Clinical, analytical, electrocardiographic and echocardiographic variables obtained during the first 24 h of admission were analysed. Results Univariate differences were found at admission in NT-ProBNP, creatinine, history of cardiac surgery, smoking and alcoholism, left and right ventricular ejection fraction, systolic blood pressure and heart rate. The ROC analysis showed significant areas under the curve for the NT-ProBNP (AUC: 0.63, 95% CI: 0.60–0.67; p < 0.001) and creatinine (AUC: 0.69, 95% CI: 0.66–0.72; p < 0.0001). The variables associated with prolonged hospital admission were NT-ProBNP (OR: 1, 95% CI: 1–1; p < 0.001), creatinine (OR: 2.2, 95% CI: 1.8–2.7; p < 0.0001) and previous smoking (OR: 1.5, 95% CI: 0.4–1; p < 0.02) Conclusions Variables such as creatinine and NT-ProBNP at hospital admission may define a subgroup of patients who will probably have a long hospital stay. Therefore, the planning of hospital care and transition to discharge may be enhanced.
Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification

HPLC is established as a fast convenient analytical technology for characterizing the content of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV-based monitoring unfortunately over-estimates the proportion of full capsids and offers little value for characterizing unpurified samples. The present study combines dual-wavelength UV monitoring with intrinsic fluorescence, extrinsic fluorescence, and light-scattering to extend the utility of HPLC for supporting development of therapeutic AAV-based drugs. Applications with anion exchange (AEC), cation exchange (CEC), and size exclusion chromatography (SEC) are presented. Intrinsic fluorescence increases sensitivity of AAV detection over UV and enables more objective estimation of empty and full capsid ratios by comparison of their respective peak areas. Light scattering enables identification of AAV capsids in complex samples, plus semiquantitative estimation of empty and full capsid ratios from relative peak areas of empty and full capsids. Extrinsic Picogreen fluorescence enables semiquantitative tracking of DNA with all HPLC methods at all stages of purification. It does not detect encapsidated DNA but reveals DNA associated principally with the exteriors of empty capsids. It also enables monitoring of host DNA contamination across chromatograms. These enhancements support many opportunities to improve characterization of raw materials and process intermediates, to accelerate process development, provide rapid in-process monitoring, and support process validation.
Adaptive Estimation Algorithm for Correcting Low-Cost MEMS-SINS Errors of Unmanned Vehicles under the Conditions of Abnormal Measurements

In this paper, a low-cost small-sized strap-down inertial navigation system (SINS)—Gyrolab GL-VG 109—is studied. When the system is installed on an unmanned vehicle and works in autonomous mode, it is difficult to determine the navigation parameters of the unmanned vehicle. Correcting the SINS information from the Global Navigation Satellite System (GNSS) can significantly increase the determination accuracy of the navigation parameters. However, this is only available when the GNSS signals are stable. A new adaptive estimation algorithm that can automatically detect, evaluate, and process the abnormal measurements is proposed in the present work. The determination of the navigation parameters can reach the third accuracy class using the proposed method. The effectiveness of the algorithm is verified by the mathematical simulation and the experimental tests (with a real SINS GL-VG 109), which are conducted in urban environments with a GNSS signal containing 15% and 40% abnormal measurements. The results show that the proposed method can significantly reduce the impact of abnormal measurements and improve the estimation accuracy.
Enhanced Recovery after Surgery (ERAS) for Hip and Knee Replacement—Why and How It Should Be Implemented Following the COVID-19 Pandemic

The COVID-19 pandemic has led to a reduction in hip and knee replacement surgery across healthcare systems. When regular operating returns, there will be a large volume of patients and an emphasis on a short hospital stay. Patients will be keen to return home, and capacity will need to maximised. Strategies to reduce the associated risks of surgery and to accelerate recovery will be needed, and so Enhanced Recovery after Surgery (ERAS) should be promoted as the model of care. ERAS protocols are proven to reduce hospital stay safely; however, ERAS pathways may require adaption to ensure both patient and staff safety. The risk of exposure to possible sources of COVID-19 should be limited, and so hospital visits should be minimised. The use of technology such as smartphone apps to provide pre-operative education, wearable activity trackers to assist with rehabilitation, and the use of telemedicine to complete outpatient appointments may be utilised. Also, units should be reminded that ERAS protocols are multi-modal, and every component is vital to minimise the surgical stress response. The focus should be on providing better and not just faster care. Units should learn from the past in order to expedite the implementation of or adaption of existing ERAS protocols. Strong leadership will be required, along with a supportive organisational culture, an inter-professional approach, and a recognised QI method should be used to contextualize improvement efforts.
Back-Gate GaN Nanowire-Based FET Device for Enhancing Gas Selectivity at Room Temperature

In this work, a TiO2-coated GaN nanowire-based back-gate field-effect transistor (FET) device was designed and implemented to address the well-known cross-sensitive nature of metal oxides. Even though a two-terminal TiO2/GaN chemiresistor is highly sensitive to NO2, it suffers from lack of selectivity toward NO2 and SO2. Here, a Si back gate with C-AlGaN as the gate dielectric was demonstrated as a tunable parameter, which enhances discrimination of these cross-sensitive gases at room temperature (20 °C). Compared to no bias, a back-gate bias resulted in a significant 60% increase in NO2 response, whereas the increase was an insignificant 10% in SO2 response. The differential change in gas response was explained with the help of a band diagram, derived from the energetics of molecular models based on density functional theory (DFT). The device geometries in this work are not optimized and are intended only for proving the concept.
A Standardized Buffalo Concussion Treadmill Test Following Sport-Related Concussion in Youth: Do ActiGraph Algorithms Matter?

CONTEXT Current recovery guidelines following sport-related concussion (SRC) include 24-48 hours of rest followed by gradual return-to-activity with heart rate (HR) maintained below symptom threshold. Additionally, the monitoring of physical activity (PA) post-SRC using ActiGraph accelerometers can provide further objective insight on amounts of activity associated with recovery trajectories. Cut-point algorithms for these devices allow minute-by-minute PA to be classified into intensity domains; however, studies have shown different algorithms employed on the same healthy participant dataset can produce varying classifications. OBJECTIVE To identify the most physiologically appropriate cut-point algorithm (Evenson or Romanzini) to analyze ActiGraph data in concussed youth with comparisons to HR response on the Buffalo Concussion Treadmill Test (BCTT). DESIGN Prospective cohort study. SETTING Sport-concussion clinic within a university setting. PATIENTS OR OTHER PARTICIPANTS Eleven high-school students (5 male, 6 female; median [range] age =16 years [15-17], height = 177.8 cm [157.5-198.1], weight = 67 kg [52-98], body mass index = 22 kg/m2 [17-31]) involved in high-risk sport who sustained a physician diagnosed SRC. MAIN OUTCOME MEASURE(S) Evenson and Romanzini algorithm PA intensity domains via ActiGraph data and HR during the BCTT. RESULTS There were differences in moderate (P = .001) and vigorous (P = .002) intensities between algorithms, but no difference in light (P = .548). Evenson classified most of the time as moderate intensity (57.03% [0.00-94.12%]), whereas Romanzini classified virtually all PA as vigorous (88.25% [2.94-97.06%]). PA based on HR (stages 1-7: 20-39% HR reserve (HRR), stages 8-13: 40-59% HRR, stages 14 and above: 60-85% HRR) indicated the BCTT primarily involves light-to-moderate intensity, and therefore is better represented by the Evenson algorithm. CONCLUSIONS The Evenson algorithm better characterizes the HR response during a standardized exercise test in concussed individuals and therefore should be used to analyze ActiGraph PA data in a concussed paediatric population.
Conversion of azide to primary amine via Staudinger reaction in metal–organic frameworks

Facile conversion of azide to primary amine in metal–organic frameworks (MOFs) was accomplished by Staudinger reduction. After the reaction, MOFs retained high crystallinity confirmed by X-ray diffraction patterns, meaning a high usability of this method for post-synthetic modification of MOFs. Bulky phosphine groups provided surface-selective modification of MOFs via a reaction between resulting amine groups of MOF and activated ester with fluorescein, illustrated by confocal laser scanning microscope (CLSM) observation. This method opens up new possibilities for the preparation of MOFs having core-shell structures.
The radical cation of N,N-diethyl-para-phenylendiamine: A possible indicator of oxidative stress in biological samples

The alkoxy and peroxy radicals formed in the degradation of hydroperoxides brought about by transition metal ions in acidic media can convert substrates with suitably low oxidation potentials, such as N,N-diethyl-para-phenylendiamine, to the corresponding radical cations. The possibility that these reactions are used in the evaluation of oxidative stress in human beings is discussed.
Three-dimensional analysis of hand coordination following tendon transfers for posterior interosseous nerve palsy

Esplugas M, Garcia-Elias M, Lluch A, Llusa Perez M. Role of muscles in the stabilization of ligament-deficient wrists. J Hand Ther. 2016, 29: 166–74. Rajan PV, Day CS. Scapholunate interosseous ligament anatomy and biomechanics. J Hand Surg Am. 2015, 40: 1692–702. Tan J, Chen J, Mu S, Tang JB, Garcia-Elias M. Length changes in scapholunate interosseous ligament with resisted wrist radial and ulnar inclination. J Hand Surg Am. 2018, 43: 482 e1–e7. Upal MA, Crisco JJ, Moore DC, Sonenblum SE, Wolfe SW. In vivo elongation of the palmar and dorsal scapholunate interosseous ligament. J Hand Surg Am. 2006, 31: 1326–32.
Microwave Sintering and Microwave Dielectric Properties of (1–x)Ca0.61La0.26TiO3-xNd(Mg0.5Ti0.5)O3 Ceramics

The (1–x)Ca0.61La0. 26TiO3-xNd(Mg0.5Ti0.5)O3 [(1–x)CLT-xNMT, x = 0.35~0.60] ceramics were prepared via microwave sintering. The effects of sintering temperature and composition on the phase formation, microstructure, and microwave dielectric properties were investigated. The results show that the microwave sintering process requires a lower sintering temperature and shorter sintering time of (1–x)CLT-xNMT ceramics than conventional heating methods. All of the (1–x)CLT-xNMT ceramics possess a single perovskite structure. With the increase of x, the dielectric constant (ε) shows a downward trend; the quality factor (Qf) drops first and then rises significantly; the resonance frequency temperature coefficient (τf) keeps decreasing. With excellent microwave dielectric properties (ε = 51.3, Qf = 13,852 GHz, τf = −1.9 × 10−6/°C), the 0.65CLT-0.35NMT ceramic can be applied to the field of mobile communications.
Performance of commercially-available cholesterol self-tests

Background Hypercholesterolemia (plasma cholesterol concentration ≥5.2 mmol/L) is a risk factor for cardiovascular disease and stroke. Many different cholesterol self-tests are readily available at general stores, pharmacies and web shops. However, there is limited information on their analytical and diagnostic performance. Methods We included 62 adult patients who required a lipid panel measurement (cholesterol, high-density lipoprotein (HDL), triglycerides and LDLcalc) for routine care. The performance of five different cholesterol self-tests, three quantitative meters (Roche Accutrend Plus, Mission 3-in-1 and Qucare) and two semi-quantitative strip tests (Veroval and Mylan MyTest), was assessed according to the manufacturers’ protocol. Results The average plasma cholesterol concentration was 5.2 ± 1.2 mmol/L. The mean absolute relative difference (MARD) of the five cholesterol self-tests ranged from 6 ± 5% (Accutrend Plus) to 20 ± 12% (Mylan Mytest). The Accutrend Plus cholesterol meter showed the best diagnostic performance with a 92% sensitivity and 89% specificity. The Qucare and Mission 3-in-1 are able to measure HDL concentrations and can thus provide a cholesterol:HDL ratio. The Passing-Bablok regression analyses for the ratio showed poor performance in both self-tests (Mission 3-in-1: y = 1.62x–1.20; Qucare: y = 0.61x + 1.75). The Accutrend Plus is unable to measure the plasma high-density lipoprotein concentration. Conclusions/interpretation: The Accutrend Plus cholesterol meter (Roche) had excellent diagnostic and analytic performance. However, several of the commercially-available self-tests had considerably poor accuracy and diagnostic performance and therefore do not meet the required qualifications, potentially leading to erroneous results. Better regulation, standardization and harmonization of cholesterol self-tests is warranted.
Advances of microfluidic intestine-on-a-chip for analyzing anti-inflammation of food

Abstract Microfluidic intestine-on-a-chip enables novel means of emulating human intestinal pathophysiology in vitro, which can potentially reduce animal testing and substitute simple 2D culture system. Though a great deal of work has been done in the development of microfluidic platforms for intestinal disease modeling and drug screening, potential investigation of the effect of bioactive food compounds on intestinal inflammation remains largely unexplored. In this review, different biomaterials and chip designs have been explored in the fabrication of intestine-on-a-chip. Other key parameters must be carefully controlled and selected, including shear stress, cell type and cell co-culture spatial configuration, etc. Appropriate techniques to quantify the barrier integrity including trans-epithelial electric resistance, specific tight junction markers and permeability measurements should be standardized and compared with in vivo data. Integration of the gut microbiome and the provision of intestinal-specific environment are the key parameters to realize the in vivo intestinal model simulation and accelerate the screening efficiency of bioactive food compounds.
Preparation of Carbon Nanotubes by Arc-Discharge Evaporation

Extremely thin graphite filaments with a tubular structure were grown in carbon deposits formed on the negative electrode during generation of fullerenes by dc carbon-arc-discharge evaporation. The filaments and small graphite particles coexist in the central portion of the deposits. Three kinds of morphologies of carbon, namely nanotubes, polyhedra and sheets were observed in transmission electron micrographs. The filaments were formed in He, Ar or CH4 gas environment at a pressure between 20-200 Torr.
Removal of aflatoxin b1 and t-2 toxin by bacteria isolated from commercially available probiotic dairy foods

This study isolated lactic acid bacteria from commercially available probiotic foods to determine their capacity to remove aflatoxin B1 (AFB1) and trichothecene-2 (T-2). The removal rates by original live and heat-treated cells of lactic acid bacteria (LAB) were compared to test the effect of heat treatment on efficacy. LAB is capable to remove up to 46% of AFB1 and up to 45% of T-2 toixn. The toxin removal capability increased as toxin concentration increased despite bacterial cell viability declining. Surprisingly, the denatured LAB removed greater percentages of AFB1 (up to 62%) and T-2 (up to 52%) than live bacterial cells (P < 0.05), lending support to the hypothesis that there is higher binding of toxins to the cell membrane of nonviable cells. The research provided practical evidences, which suggest that when ingested into the gut biota, LAB could likely reduce absorption of AFB1 and T-2 from contaminated foods.
Quantitative Evaluations of Hydrogen Diffusivity in V-X (X = Cr, Al, Pd) Alloy Membranes Based on Hydrogen Chemical Potential

Vanadium (V) has higher hydrogen permeability than Pd-based alloy membranes but exhibits poor resistance to hydrogen-induced embrittlement. The alloy elements are added to reduce hydrogen solubility and prevent hydrogen-induced embrittlement. To enhance hydrogen permeability, the alloy elements which improve hydrogen diffusivity in V are more suitable. In the present study, hydrogen diffusivity in V-Cr, V-Al, and V-Pd alloy membranes was investigated in view of the hydrogen chemical potential and compared with the previously reported results of V-Fe alloy membranes. The additions of Cr and Fe to V improved the mobility of hydrogen atoms. In contrast, those of Al and Pd decreased hydrogen diffusivity. The first principle calculations revealed that the hydrogen atoms cannot occupy the first-nearest neighbor T sites (T1 sites) of Al and Pd in the V crystal lattice. These blocking effects will be a dominant contributor to decreasing hydrogen diffusivity by the additions of Al and Pd. For V-based alloy membranes, Fe and Cr are more suitable alloy elements compared with Al and Pd in view of hydrogen diffusivity.
Wildfire Risk Assessment of Transmission-Line Corridors Based on Naïve Bayes Network and Remote Sensing Data

Considering the complexity of the physical model of wildfire occurrence, this paper develops a method to evaluate the wildfire risk of transmission-line corridors based on Naïve Bayes Network (NBN). First, the data of 14 wildfire-related factors including anthropogenic, physiographic, and meteorologic factors, were collected and analyzed. Then, the relief algorithm is used to rank the importance of factors according to their impacts on wildfire occurrence. After eliminating the least important factors in turn, an optimal wildfire risk assessment model for transmission-line corridors was constructed based on the NBN. Finally, this model was carried out and visualized in Guangxi province in southern China. Then a cost function was proposed to further verify the applicability of the wildfire risk distribution map. The fire events monitored by satellites during the first season in 2020 shows that 81.8% of fires fall in high- and very-high-risk regions.