Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial Distribution of Dissolved Inorganic Nutrients and Phytoplankton around Kota Kinabalu Wetland, Sabah, Malaysia

Kota Kinabalu Wetland is importantly habited of mangrove, diverse aquatic flora and fauna as well as feeding stop for migratory birds. This wetland is inundated with the tidal flow, as connected with a small river and nearby coastal areas, thus. A study was carried out to determine the spatial distribution of dissolved inorganic nutrients and phytoplankton diversity at Kota Kinabalu (KK) Wetlands. Five stations, in which river mouth of Likas Bay, river channel (two stations) and inundated area (two stations) in KK Wetland were selected for this study. In-situ parameters of water, water for nutrients and phytoplankton samples were collected from May 2019 until October 2019. The highest concentration of nitrate (0.115 mg/L) was recorded at inundated area of wetland (S5) while the lowest nitrate concentration (0.0047 mg/L) was found at river (S3) flowing towards wetland. The concentrations of ammonia (0.2004 to 2.311 mg/L) were recorded relatively higher at every station compared to other dissolved inorganic nutrients (DIN). The concentration of phosphate was determined in the ranges of 0.0089 - 0.0513 mg/L. Nitrate, ammonia and phosphate showed no significant difference (P = 0.737) in terms of DIN concentration at all five sampling stations during the study period. Twenty-four genera of phytoplankton were identified, dominated by diatoms (55.29%), followed by dinoflagellates (24.95%), Chrysophyta (11.15%), Spirotrichea (5.28%) and Cyanophyta (3.33%). Dominating species throughout the study period include Chaetoceros sp. , Pseduo-nitzschia sp. , and Cylindrotheca closterium, Peridinium quinquecorne and Alexandrium sp. Phytoplankton species compositions were observed the highest in river mouth area in July with the highest density of 12.115 × 104 cells/mL. The study showed that nutrient concentration was insignificant (P = 0.614) in altering the phytoplankton density, as influences with the tidal water.
The impact of high hydrostatic pressure (40 MPa and 60 MPa) on the apoptosis rates and functional activity of cryopreserved porcine mesenchymal stem cells

Abstract The aim of the present study was to examine the influence of two varied high hydrostatic pressure (HHP) values on the apoptosis (assessing caspase-8, survivin, CAD, Bax, BclxL and BclxS) and functional activity (using cocultures with bovine embryos) of porcine mesenchymal stem cells (pBMSCs). pBMSCs were isolated from porcine bone marrow and cultured in vitro. Before cryopreservation and storage in liquid nitrogen, pBMSCs were subjected to HHP values of 40 MPa and 60 MPa for 1 h at 24°C. After thawing, the cells were analysed for caspase-8 activity and protein expression of survivin, CAD, Bax, BclxL and BclxS. To indirectly test the influence of HHP on the functional activity of pBMSCs, in vitro maturated bovine oocytes were fertilized in vitro, and the obtained embryos were cultured under 4 different conditions: 1. monoculture in SOF medium; 2. coculture with pBMSCs in SOF medium; 3. coculture with pBMSCs subjected to 40 MPa HHP in SOF medium and 4. coculture with pBMSCs subjected to 60 MPa HHP in SOF medium. The quality of the developed blastocysts was analysed by TUNEL assay. HHP did not induce apoptosis in pBMSCs, as no significant difference was noted in the expression of any of the analysed apoptosis- related proteins between pBMSCs subjected to HHP (40 MPa or 60 MPa) and control. The highest number of obtained blastocysts was observed when the embryos were cultured in SOF. A highly significant difference (P<0.005) was noted between embryos cultured in SOF and embryos cultured in the presence of pBMSCs subjected to 60 MPa HHP or untreated pBMSCs. A significant difference (P<0.05) was noted between embryos cultured in SOF and embryos cultured in the presence of pBMSCs subjected to 40 MPa HHP. In conclusion, HHP does not induce apoptosis in pBMSCs. The obtained results of the blastocysts cocultured in vitro with pBMSCs (HHP-treated and untreated cells) imply that coculture with pBMSCs has a negative impact on the developmental rates of blastocysts.
Transient Expression of Coat Protein of Foot and Mouth Disease Virus (FMDV) in Alfalfa (Medicago sativa) by Agroinfiltration

An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. Moreover, gene expression in the transformed leaves was quantified by ELISA method. The results obtained in this investigation indicated high level of gene expression in alfalfa leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.
Solvent-Free Mechanochemical Approach towards Thiospinel MgCr2S4 as a Potential Electrode for Post-Lithium Ion Batteries

Several new compounds, with desirable properties of ion mobility and working voltage, have been recently proposed using a density functional theory (DFT) computational approach as potential electrode materials for beyond-lithium battery systems. After evaluation of the ‘energy above hull’, thiospinel MgCr2S4 has been suggested as interesting multivalent battery cathode candidate, even though the synthesis of its exact stoichiometry poses serious challenges. In this work, MgCr2S4 is prepared using an innovative mechanochemical route starting from magnesium or magnesium hydride, chromium, and sulfur powders. The progress of such mechanically induced reaction as a function of processing time is carefully monitored by XRD with Rietveld refinement, evidencing the occurrence of a mechanically induced self-propagating reaction (MSR). The effect of parameters associated with the milling apparatus (impact energy) on the products composition are also investigated. To our knowledge, this work represents the first report of the scalable and simple mechanical alloying synthesis of thiospinel MgCr2S4 (space group Fd-3 m, a = 10.09 Å) and opens up interesting possibilities for the exploitation of such material in next-generation post-lithium batteries.
IMPACT OF PRENATAL EXPOSURE TO OBESOGENIC ENVIRONMENT ON LATER-LIFE BROWN AND WHITE ADIPOCYTES AND LIVER OUTCOMES

Materials and methods: The study evaluated female rats (n=20) and their male offspring (n=92). Pregnant rats were exposed to social stress (Pratt, 1989) during the fi rst and third trimester and standard diet (StD, control), nutritional imbalance: high-sugar (Kozar, 2009), high-fat diets (Lintermans, 2011) or their combination – high-sugar and high-fat diet (HSFD). The effects of maternal stress and nutrition on the BAT, WAT and liver were evaluated in 3-month offspring during stress-exposure (Takagi, 1964) without and with abnormal cellular defence (treatment of indomethacin), along with the ELISA levels of IL1β, IL-8, leptin (OB), adiponectin (AdipoQ) and its ratio (OB/AdipoQ index). Results: Maternal exposure to stress in combination with the HSFD resulted in the most prominent histological changes in offspring’s BAT and WAT infl ammation and hepatocellular damage. Such effects were accompanied by most signifi cant changes: increased IL (interleukin)-1β (178%, p ≤ 0.001), IL-8 (57%, p ≤ 0.001), leptin (OB) (34%, p ≤ 0.001) and decrease of adiponectin (Adipoq) (53%, p ≤ 0.001) vs control. In cases of combination of stress and indomethacin injury we noticed an increase of IL-1β (213%, p ≤ 0.001), IL-8 (27%), OB (30%, p ≤ 0.001) as well as decrease of AdipoQ (64%, p ≤ 0.001) vs control. Conclusion: The impairment in the BAT, WAT and liver function in the offspring was related to the sugar overload and stress during the prenatal period. Ectopic fat accumulation, hyperplastic and hypertrophic WAT changes, intracellular lipid and proinfl ammatory secretion, and the OB/AdipoQ index may be a predictive biomarkers of NAFLD.
Mosquito control using Badis badis (Hamilton, 1822) [Teleostei: Perciformes] in ground water (domestic well): Preliminary findings of a laboratory trial.

Food preference and predation potentiality experiments using Badia badis (Hamilton) was studied under laboratory conditions to destroy the mosquito breeding ground in domestic well at Jalpaiguri District of West Bengal. The fish were sampled from the water bodies of Moraghat forest and were maintained in two glass aquariums with chemically untreated well water which was sieved through a phytoplankton net to exclude larvae and planktons. To determine the feeding preference on mosquito larvae, selected fish were kept over night before experiment for acclimatization and starvation. Mosquito larvae were collected several wells and introduced in first aquarium. Remaining larvae were counted every 3 hours interval from 6 am to 6 pm and after counting the number of consumed larvae at every 3-hour interval, the same numbers of larvae were replenished within the glass beaker to maintain the constant prey density. The authors conducted a similar experiment to determine the feeding preference on commercially available fish pellets. In this experiment, formulated fish pellets were given initially in second aquarium and remaining pellets were counted every 3 hours interval from 6 am to 6 pm. The experiment showed greater feeding preference for mosquito larvae than formulated fish diet. The predation potentiality or feeding capability result showed a 3.6 cm length fish could consume 29 larvae per day with predatory index 37.66. The present study concludes that introduction of indigenous Badis badis can effectively reduce mosquito larvae in domestic well (groundwater) and also suggests that this species may help to prevent the mosquito borne diseases biologically.
Life Cycle Assessment of Variable Rate Fertilizer Application in a Pear Orchard

Precision Agriculture (PA) is a crop site-specific management system that aims for sustainability, adopting agricultural practices more friendly to the environment, like the variable rate application (VRA) technique. Many studies have dealt with the effectiveness of VRA to reduce nitrogen (N) fertilizer, while achieving increased profit and productivity. However, only limited attention was given to VRA’s environmental impact. In this study an International Organization for Standardization (ISO) based Life Cycle Assessment (LCA) performed to identify the environmental effects of N VRA on a small pear orchard, compared to the conventional uniform application. A Cradle to Gate system with a functional unit (FU) of 1 kg of pears was analyzed including high quality primary data of two productive years, including also the non-productive years, as well as all the emissions during pear growing and the supply chains of all inputs, projecting them to the lifespan of the orchard. A methodology was adopted, modelling individual years and averaging over the orchard’s lifetime. Results showed that Climate change, Water scarcity, Fossil fuels and Particulate formation were the most contributing impact categories to the overall environmental impact of the pear orchard lifespan, where climate change and particulates were largely determined by CO2, N2O, and NH3 emissions to the air from fertilizer production and application, and as CO2 from tractor use. Concerning fertilization practice, when VRA was combined with a high yield year, this resulted in significantly reduced environmental impact. LCA evaluating an alternative fertilizer management system in a Greek pear orchard revealed the environmental impact reduction potential of that system.
Flocculation Performance and Kinetics of Magnetic Polyacrylamide Microsphere under Different Magnetic Field Strengths

In this study, the flocculation performance and kinetics of magnetic cationic polyacrylamide (MCPAM) microspheres, compared with cationic polyacrylamide (CPAM), were systematically investigated under different magnetic field strengths. Flocculation performance was observed by jar test experiment. The density of flocs was estimated by the determination of floc settlement velocity and image analysis. The frequency distribution of floc size was measured with a Malvern Mastersizer instrument. When the diatomite suspension was treated by MCPAM and CPAM, the residual diatomite turbidity was 16.28 NTU and 244.13 NTU, respectively. The maximum turbidity removal efficiency of MCPAM was about 99.65% under 1000 Gauss magnetic field, which was higher than that (94.75%) of CPAM. The synergy of gravitational and magnetic fields for MCPAM promoted the formation of larger flocs with higher growth rates compared with CPAM. The effective density range of flocs in the MCPAM flocculation was increased to 10–252 kg m−3. The kinetic constants were calculated by monitoring the frequency of floc collisions. The increase of kinetic constant (k) to 25.81 × 10−11 s−1 suggested that interaction of contact and collision between magnetic flocs was sufficient. According to the evolution of the size and density of flocs under the synergy of gravitational and magnetic fields, the magnetic flocculation rate equation dN/dt=−1/9μρ−ρlg+ρkmHdH/dXai2−aj2−ai2e9μt/2ai2ρ+aj2e9μt/2aj2ρai+aj2 was derived. The study of magnetic flocculation kinetics can provide theoretical support for magnetic flocculation and is critical for the analysis of solid-liquid separation processes.
Physiological and biochemical responses of Emiliania huxleyi to ocean acidification and warming are modulated by UV radiation

Abstract. Marine phytoplankton such as bloom-forming, calcite-producing coccolithophores, are naturally exposed to solar ultraviolet radiation (UVR, 280–400 nm) in the ocean's upper mixed layers. Nevertheless, the effects of increasing carbon dioxide (CO2)-induced ocean acidification and warming have rarely been investigated in the presence of UVR. We examined calcification and photosynthetic carbon fixation performance in the most cosmopolitan coccolithophorid, Emiliania huxleyi, grown under high (1000 µatm, HC; pHT: 7.70) and low (400 µatm, LC; pHT: 8.02) CO2 levels, at 15 ∘C, 20 ∘C and 24 ∘C with or without UVR. The HC treatment did not affect photosynthetic carbon fixation at 15 ∘C, but significantly enhanced it with increasing temperature. Exposure to UVR inhibited photosynthesis, with higher inhibition by UVA (320–395 nm) than UVB (295–320 nm), except in the HC and 24 ∘C-grown cells, in which UVB caused more inhibition than UVA. A reduced thickness of the coccolith layer in the HC-grown cells appeared to be responsible for the UV-induced inhibition, and an increased repair rate of UVA-derived damage in the HC–high-temperature grown cells could be responsible for lowered UVA-induced inhibition. While calcification was reduced with elevated CO2 concentration, exposure to UVB or UVA affected the process differentially, with the former inhibiting it and the latter enhancing it. UVA-induced stimulation of calcification was higher in the HC-grown cells at 15 and 20 ∘C, whereas at 24 ∘C observed enhancement was not significant. The calcification to photosynthesis ratio (Cal ∕ Pho ratio) was lower in the HC treatment, and increasing temperature also lowered the value. However, at 20 and 24 ∘C, exposure to UVR significantly increased the Cal ∕ Pho ratio, especially in HC-grown cells, by up to 100 %. This implies that UVR can counteract the negative effects of the “greenhouse” treatment on the Cal ∕ Pho ratio; hence, UVR may be a key stressor when considering the impacts of future greenhouse conditions on E. huxleyi.
Evangelical indigenous radio stations in Colombia: Between the promotion of social change and religious indoctrination

This article refutes dominant views that define evangelical indigenous media as intrinsic tools for religious indoctrination. The case of the Colombian Misak community shows that evangelical radio stations can contribute to community building. However, the degree of the positive or negative contribution of evangelical media depends on the dominance of evangelical presence at indigenous localities. The rapid expansion of indigenized evangelical groups via the provision of social services has radicalized Evangelicals against views different from their own. As a result, these evangelical media are progressively leaving their role as promoters of positive social change to become tools for religious indoctrination.
Species traits explain long‐term population trends of Finnish cuckoo wasps (Hymenoptera: Chrysididae)

Kleptoparasitic and parasitoid insects are expected to be particularly sensitive to changes in habitat availability due to their high trophic position and small population sizes compared with their hosts, but there are only few quantitative studies on their population changes. Here, we studied the distribution and abundance of 48 kleptoparasitic and parasitoid species of cuckoo wasps (Chrysididae) and eight selected host species recorded in Finland from 1840 to 2015 based on an extensive survey of entomological collections. Population trends were assessed by studying changes in occupancy in 10 × 10 km grid squares between two study periods, 1840–1967 and 1968–2015. Statistically significant decreases in occurrence were found for 11 cuckoo wasp species and one host species, while significant increases were not observed for any species. Trends of cuckoo wasps and their hosts were positively correlated, and changes were generally stronger in cuckoo wasps than in their hosts. In a comparative analysis of species traits, abundance, body size and nesting type of host were related to occurrence changes of cuckoo wasps. Scarce and small species that use above ground‐nesting hosts declined more than abundant and large species that use ground‐nesting hosts. Cuckoo wasp species dependent on dead wood are more vulnerable to changes in the environment than species associated with open sandy habitats. While both groups of species have probably suffered from habitat loss, the emergence of secondary habitats may have benefitted species living in sandy areas and compensated for the negative impact of habitat destruction.
VOC Monitoring and Ozone Generation Potential Analysis Based on a Single-Photon Ionization Time-of-Flight Mass Spectrometer

The single-photon ionization time-of-flight mass spectrometer (SPI-TOFMS), which has high sensitivity, high accuracy, and a short response time, is effective for the real-time monitoring of volatile organic compounds (VOCs). In this study, the theory and structural composition of the SPI-TOFMS are described. Its detection limit can reach parts per billion by volume (ppbv), the dynamic range is better than three orders of magnitude, and the response speed can reach milliseconds. The distribution of VOCs and the ozone generation contribution rate in the Hefei Economic Development Zone are analyzed using this instrument with a vehicle platform for online navigation detection. The experimental results showed that aromatics were the primary components of the total volatile organic compounds (TVOCs), and aromatics and alkenes contributed more to ozone formation in an industrial manufacturing area. This research indicates that the SPI-TOFMS can rapidly and accurately conduct online monitoring of VOCs in industrial development zones. In addition, it has been applied in the fields of atmospheric composition observation, environmental monitoring, and industrial VOC leakage monitoring.
Handbook of the mammals of the world : vol. 3 : primates

Everybody knows that humans are members of the mammalian Order Primates, but ask somebody to name some of our cousins and they would at best come up with five or six of the most widely known. This volume counts more than 470 of them: 138 species of prosimiansthe lemurs, lorises, pottos and tarsiers in Africa and Asia; 156 species of monkeys in the New World tropics; another 158 monkeys in the Old World tropics; and 25 apes, 19 of them gibbons along with six chimpanzees, gorillas and orangutans. This publication presents these species in all their remarkable variety of shapes, sizes, colors, habits and specializations.
Nationwide revisitation reveals thousands of local extinctions across the ranges of 713 threatened and rare plant species

Despite increasing awareness of global biodiversity loss, we lack quantitative data on local extinctions for many species. This is especially true for rare species, which are typically assessed on the basis of expert judgment rather than data. Revisiting previously assessed populations enables estimation of local extinction rates and the identification of species characteristics and habitats with high local extinction risk. Between 2010 and 2016, in a nationwide revisitation study, 420 volunteer botanists revisited 8,024 populations of the 713 rarest and most threatened plant species in Switzerland recorded between 1960 and 2001. Of the revisited 8,024 populations, 27% had gone locally extinct. Among critically endangered species, the local extinctions increased to 40%. Species from ruderal and freshwater habitat types showed the highest proportion of local extinctions. Our results provide compelling evidence for rapid and widespread local extinctions and suggest that current conservation measures are insufficient. Local extinctions precede and provide early warnings for global extinctions. The ongoing loss of populations suggests that we will lose species diversity unless we scale up species‐targeted conservation and restoration measures, especially in anthropogenic landscapes.
A BiLSTM-CNN model for predicting users’ next locations based on geotagged social media

ABSTRACT Location prediction based on spatio-temporal footprints in social media is instrumental to various applications, such as travel behavior studies, crowd detection, traffic control, and location-based service recommendation. In this study, we propose a model that uses geotags of social media to predict the potential area containing users’ next locations. In the model, we utilize HiSpatialCluster algorithm to identify clustering areas (CAs) from check-in points. CA is the basic spatial unit for predicting the potential area containing users’ next locations. Then, we use the LINE (Large-scale Information Network Embedding) to obtain the representation vector of each CA. Finally, we apply BiLSTM-CNN (Bidirectional Long Short-Term Memory-Convolutional Neural Network) for location prediction. The results show that the proposed ensemble model outperforms the single LSTM or CNN model. In the case study that identifies 100 CAs out of Weibo check-ins collected in Wuhan, China, the Top-5 predicted areas containing next locations amount to an 80% accuracy. The high accuracy is of great value for recommendation and prediction on areal unit.