Miracle-dz/UniFAR-FTUs huggingface dataset:
Brain Testosterone-CYP1B1 (Cytochrome P450 1B1) Generated Metabolite 6β-Hydroxytestosterone Promotes Neurogenic Hypertension and Inflammation

Supplemental Digital Content is available in the text. Previously, we showed that peripheral administration of 6β-hydroxytestosterone, a CYP1B1 (cytochrome P450 1B1)-generated metabolite of testosterone, promotes angiotensin II-induced hypertension in male mice. However, the site of action and the underlying mechanism by which 6β-hydroxytestosterone contributes to angiotensin II-induced hypertension is not known. Angiotensin II increases blood pressure by its central action, and CYP1B1 is expressed in the brain. This study was conducted to determine whether testosterone-CYP1B1 generated metabolite 6β-hydroxytestosterone locally in the brain promotes the effect of systemic angiotensin II to produce hypertension in male mice. Central CYP1B1 knockdown in wild-type (Cyp1b1+/+) mice by intracerebroventricular-adenovirus-GFP (green fluorescence protein)-CYP1B1-short hairpin (sh)RNA attenuated, whereas reconstitution of CYP1B1 by adenovirus-GFP-CYP1B1-DNA in the paraventricular nucleus but not in subfornical organ in Cyp1b1−/− mice restored angiotensin II-induced increase in systolic blood pressure measured by tail-cuff. Intracerebroventricular-testosterone in orchidectomized (Orchi)-Cyp1b1+/+ but not in Orchi-Cyp1b1−/−, and intracerebroventricular-6β-hydroxytestosterone in the Orchi-Cyp1b1−/− mice restored the angiotensin II-induced: (1) increase in mean arterial pressure measured by radiotelemetry, and autonomic imbalance; (2) reactive oxygen species production in the subfornical organ and paraventricular nucleus; (3) activation of microglia and astrocyte, and neuroinflammation in the paraventricular nucleus. The effect of intracerebroventricular-6β-hydroxytestosterone to restore the angiotensin II-induced increase in mean arterial pressure and autonomic imbalance in Orchi-Cyp1b1−/− mice was inhibited by intracerebroventricular-small interfering (si)RNA-androgen receptor (AR) and GPRC6A (G protein-coupled receptor C6A). These data suggest that testosterone-CYP1B1-generated metabolite 6β-hydroxytestosterone, most likely in the paraventricular nucleus via AR and GPRC6A, contributes to angiotensin II-induced hypertension and neuroinflammation in male mice.
Objective measurement of physical activity and sedentary behavior among South Asian adults: A systematic review

Background South Asians are one of the fastest growing ethnic groups in western countries with a high incidence of chronic diseases like metabolic syndrome and cardiovascular disease occurring at younger ages and lower body weight compared with white Europeans. Physically active lifestyle and reduced sedentary time are modifiable risk factors that can decrease burden of chronic diseases. Population-level surveys based on self-report show South Asians engage in low levels of physical activity. Because of known limitations with self-report data, we aimed to synthesize available evidence to generate a physical activity /sedentary time profile of South Asians from studies using accelerometry. Methods We systematically searched Medline, EMBASE, CINAHL, PsycINFO, and SportDiscus. We included studies applying accelerometry to measure physical activity /sedentary time under free-living. Studies with an exclusive focus on drugs or including participants with health conditions/physical disability, and special populations (athletes/pregnant women) were excluded. Two authors independently adjudicated inclusion of citations at title/abstract and full text. We applied a standardized data abstraction form to extract relevant data. We evaluated methodological quality using Newcastle Ottawa Quality Assessment Scale. Due to variability and inconsistencies in measurement and reporting of physical activity /sedentary time, we only provide a narrative synthesis. Findings We identified only 14 studies(n = 1,338). Despite using similar accelerometry assumptions, we noted variability in reported outcomes for physical activity and sedentary time. Sedentary time ranged from 482(98) to 587 min/day. Mean light physical activity ranged from 211.69(67. 38) to 574(227) min/day. Moderate to vigorous physical activity among South Asian women ranged from 17–41 min/day and among men, 32–43 min/day. Conclusion South Asians exhibited higher levels of physical activity when compared to the Canadian population level survey but not when compared to the American population level survey. Overall, fewer studies, and small sample sizes led to considerable variability limiting any effective comparisons. Results highlight the importance of conducting methodologically robust studies based on random sampling to advance the field, and to capture true levels of sedentary time and physical activity in the South Asian population.
A New Volumetric Fusion Strategy with Adaptive Weight Field for RGB-D Reconstruction

High-quality 3D reconstruction results are very important in many application fields. However, current texture generation methods based on point sampling and fusion often produce blur. To solve this problem, we propose a new volumetric fusion strategy which can be embedded in the current online and offline reconstruction framework as a basic module to achieve excellent geometry and texture effects. The improvement comes from two aspects. Firstly, we establish an adaptive weight field to evaluate and adjust the reliability of data from RGB-D images by using a probabilistic and heuristic method. By using this adaptive weight field to guide the voxel fusion process, we can effectively preserve the local texture structure of the mesh, avoid wrong texture problems and suppress the influence of outlier noise on the geometric surface. Secondly, we use a new texture fusion strategy that combines replacement, integration, and fixedness operations to fuse and update voxel texture to reduce blur. Experimental results demonstrate that compared with the classical KinectFusion, our approach can significantly improve the accuracy in geometry and texture clarity, and can achieve equivalent texture reconstruction effects in real-time as the offline reconstruction methods such as intrinsic3d, even better in relief scenes.
Opinion on the follow‐up of the re‐evaluation of sorbic acid (E200) and potassium sorbate (E202) as food additives

Abstract In this opinion, the EFSA Panel on Food Additives and Flavourings (FAF Panel) was requested by the European Commission to carry out a scientific evaluation of an extended one‐generation reproductive toxicity study (EOGRTS) to determine whether it would allow reconsideration of the temporary group acceptable daily intake (ADI) for sorbic acid (E 200) and potassium sorbate (E 202), established by the Panel on Food Additives and Nutrient Sources added to Food (ANS Panel) in 2015. From the EOGTRS, the FAF Panel identified a lower confidence limit of the benchmark dose (BMDL) of 1,110 mg sorbic acid/kg body weight (bw) per day. By applying a default uncertainty factor of 100, the Panel established a group ADI expressed as 11 mg sorbic acid/kg bw per day for sorbic acid (E 200) and its potassium salt (E 202). In addition, European Commission asked EFSA to review a report on the ‘Stability of sorbic acid (E 200) and its potassium salt (E 202) during food processing and storage’ provided by industry. No new information was provided in this report, and therefore, in this opinion, there was no re‐assessment of the EFSA ANS opinion conclusions from 2015 regarding the stability of sorbates in food.
Coxsackievirus A6-induced Hand-Foot-and-Mouth Disease Mimicking Stevens-Johnson Syndrome in an Immunocompetent Adult

Hand-foot-and-mouth disease, a highly contagious viral infection, occurs more common in children than in adults. However, there was a recent outbreak of Coxsackievirus A6-induced infection with an atypical presentation among the adult population. Stevens–Johnson syndrome is a severe mucocutaneous disease characterized by extensive necrosis and detachment of the epidermis, and this condition is commonly caused by medications. Herein, we describe a 30-year-old male patient taking allopurinol for the management of gout. The patient presented with numerous erythematous papules, vesicles, and patches with mucosal eruptions on the whole body, oral mucositis, and fever, and he was finally diagnosed with hand-foot-and-mouth disease.
Assessment of macroglossia as a cause of failed continuous positive airway pressure adherence

Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, Australia; Department of Otolaryngology, ^The Wollongong Hospital, Wollongong, Australia; University of Queensland, St. Lucia, Queensland, Australia; Illawarra ENT Head and Neck Clinic, Wollongong, New South Wales, Australia; Department of Respiratory Medicine, ^The Wollongong Hospital, Wollongong, Australia; Illawarra Sleep Medicine Centre, Wollongong, Australia; PRP Diagnostic Imaging, Wollongong, Australia; School of Medicine, University of Wollongong, Wollongong, Australia; Woolcock Institute of Medical Research, Sydney, Australia Contributions: (I) Conception and design: ME Lam, L Kitipornchai, SG MacKay; (II) Administrative support: ME Lam; (III) Provision of study materials or patients: AC Jones, AJ Petersen, SG MacKay; (IV) Collection and assembly of data: ME Lam, L Kitipornchai, L Chan, L Sarkissian, NJ Hayward; (V) Data analysis and interpretation: ME Lam, NJ Creber; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Matthew E. Lam, MBBS. Department of Otolaryngology, The Wollongong Hospital, 66 Kyle Parade, Kyle Bay NSW 2221, Australia. Email: lam.me. 323@gmail.com.
Provider Engagement in Radiation Oncology Data Science: Workshop Report.

Anshu K. Jain, MD; Sanjay Aneja, MD; Clifton D. Fuller, MD, PhD; Adam P. Dicker, MD, PhD; Caroline Chung, MD, MSc, CIP; Erika Kim, PhD; Justin S. Kirby, BS; Harry Quon, MD, MS; Clara J. K. Lam, PhD, MPH; William C. Louv, PhD; Chris Ahern, PhD; Ying Xiao, PhD; Todd R. McNutt, PhD; Nadine Housri, MD; Ronald D. Ennis, MD; John Kang, MD, PhD; Ying Tang, PhD; Howard Higley, PhD; Michelle A. Berny-Lang, PhD; and Kevin A. Camphausen, MD
Hyaluronic Acid‐Based Bioink Composition Enabling 3D Bioprinting and Improving Quality of Deposited Cartilaginous Extracellular Matrix

In 3D bioprinting, bioinks with high concentrations of polymeric materials are frequently used to enable fabrication of 3D cell‐hydrogel constructs with sufficient stability. However, this is often associated with restricted cell bioactivity and an inhomogeneous distribution of newly produced extracellular matrix (ECM). Therefore, this study investigates bioink compositions based on hyaluronic acid (HA), an attractive material for cartilage regeneration, which allow for reduction of polymer content. Thiolated HA and allyl‐modified poly(glycidol) in varying concentrations are UV‐crosslinked. To adapt bioinks to poly(ε‐caprolactone) (PCL)‐supported 3D bioprinting, the gels are further supplemented with 1 wt% unmodified high molecular weight HA (hmHA) and chondrogenic differentiation of incorporated human mesenchymal stromal cells is assessed. Strikingly, addition of hmHA to gels with a low polymer content (3 wt%) results in distinct increase of construct quality with a homogeneous ECM distribution throughout the constructs, independent of the printing process. Improved ECM distribution in those constructs is associated with increased construct stiffness after chondrogenic differentiation, as compared to higher concentrated constructs (10 wt%), which only show pericellular matrix deposition. The study contributes to effective bioink development, demonstrating dual function of a supplement enabling PCL‐supported bioprinting and at the same time improving biological properties of the resulting constructs.
VIBRATION POWERED PIEZOELECTRIC GENERATOR USING FINITE ELEMENT METHOD JYOTI

Energy harvesting is a renewable and green power generation and is key technology of future having wide application in wireless Sensors, NEMS, MEMS, UAV, medical implant devices, health monitoring and many more (1). Approach used in this model is simple based on basic piezoelectric, beam theory. We applied this model to predict the power generated from a bimorph cantilever beam with harmonic oscillations using PZT as a piezoelectric material to supply output power to wireless sensor node. The model used to develop a genetic algorithm for optimization of an energy harvester design. The existing designs meet all engineering constraints and can be utilized for other applications as well. These results are validated to a certain degree with finite element analysis.
Leucine acutely potentiates glucose-stimulated insulin secretion in fetal sheep.

A nine-day infusion of leucine into fetal sheep potentiates fetal glucose-stimulated insulin secretion (GSIS). However, there were accompanying pancreatic structural changes that included a larger proportion of β-cells and increased vascularity. Whether leucine can acutely potentiate fetal GSIS in vivo before these structural changes develop is unknown. The mechanisms by which leucine acutely potentiates GSIS in adult islets and insulin secreting cell lines are well known. These mechanisms involve leucine metabolism including leucine oxidation. However, it is not clear if leucine-stimulated metabolic pathways are active in fetal islets. We hypothesized that leucine would acutely potentiate GSIS in fetal sheep and that isolated fetal islets are capable of oxidizing leucine. We also hypothesized that leucine would stimulate other metabolic pathways associated with insulin secretion. In pregnant sheep we tested in vivo GSIS with and without an acute leucine infusion. In isolated fetal sheep islets we measured leucine oxidation with a [1-14C]L-leucine tracer. We also measured concentrations of other amino acids, glucose, and analytes associated with cellular metabolism following incubation of fetal islets with leucine. In vivo, a leucine infusion resulted in glucose stimulated insulin concentrations that were over 50% higher than controls (P<0.05). Isolated fetal islets oxidized leucine. Leucine supplementation of isolated fetal islets also resulted in significant activation of metabolic pathways involving leucine and other amino acids. In summary, acute leucine supplementation potentiates fetal GSIS in vivo, likely through pathways related to the oxidation of leucine and catabolism of other amino acids.
POLLINATOR-MEDIATED SELECTION ON THE NECTAR GUIDE PHENOTYPE IN THE ANDEAN MONKEY FLOWER, MIMULUS LUTEUS

Mimulus luteus (Scrophulariaceae) is a perennial herb occurring in the South American Andes that shows a wide variation in the size and shape of a red spot on the lower lobe of the yellow flower. We describe the preference of four insects (three bees and one butterfly) and one hummingbird species for floral characters, and estimated the strength, direction, and form of pollinator-mediated selection through female fitness. We applied geometric morphometrics to describe the preference of pollinator species for different guide shapes. Our results revealed striking differences in the floral phenotypes preferred by insects and hummingbirds. Insects visited flowers with corollas 1.25-fold larger and guides 1.72-fold larger than the hummingbird species did. While insects preferred flowers with nectar guides pointing toward the corolla tube, the hummingbird preferred flowers with heart-shaped nectar guides. Most of the floral preferences shown by pollinators translated into significant linear and nonlinear selection coefficients. When selection was analyzed on a per-flower basis and for female fitness, corolla size was under positive directional selection, and nectar guide size and shape were under disruptive selection. Because the insect and hummingbird pollinators showed a strong segregation in their daily activity time, we suggest that current disruptive selection on the nectar guide phenotype can result from the differential availability of the rewarding floral variants over a day. Our findings suggest that pollinator-mediated selection favoring extreme phenotypes in M. luteus may not only contribute to high nectar guide variation found in this species, but also can promote divergence of corolla and nectar guide traits.
Efficacy of a Low-cost Multidisciplinary Team-led Experiential Workshop
 for Public Health Midwives on Dysphagia Management for Children with
 Cerebral Palsy

Purpose: Over the past decade there has been a growing focus on offering appropriate training to healthcare professionals and caregivers to support safe feeding practices for children with cerebral palsy. Early and consistent multidisciplinary intervention is required to minimise the risks of aspiration pneumonia. The high incidence of complications from aspiration pneumonia among children with cerebral palsy in Sri Lanka has made it necessary to conduct low-cost multidisciplinary team-led dysphagia awareness workshops for healthcare professionals and caregivers. Method: A group of 38 Public Health Midwives (PHMs) was offered an experiential workshop by a small multidisciplinary team (MDT). To determine changes in knowledge, a self-administered questionnaire that included a video-based client scenario was administered pre- and post-workshop. The data were analysed statistically using non-parametric within-participant t-tests. Results: The post-workshop responses to the questionnaire indicated a significant increase in the level of knowledge. This included positive changes in the understanding and knowledge of cerebral palsy (t (37) =-7.44, p=. 000), effects of cerebral palsy on eating and drinking skills (t (37) =-3.91, p=. 000), positioning (t (37) = -9.85, p=. 000), aspiration (t (37) =-3.46, p=. 001), food categorisation (t (37), -3.85, p=. 000) and client video observation (t (37)-3.91, p=. 000) at a p=. 05 level of significance. While there was also an increase in the knowledge on general guidelines during mealtimes, this did not reach statistical significance. Conclusion: The low-cost MDT-led experiential workshop was effective in increasing knowledge of feeding and dysphagia-related issues in cerebral palsy among a group of PHMs. This workshop could serve as a model for training PHMs and Community-Health Workers across the country in order to reach the Sustainable Development Goal of ‘good health and well-being’ for children with cerebral palsy and all children experiencing feeding difficulties. Follow-up workshops and continued professional development courses for midwives on dysphagia care are strongly recommended, in addition to collaborative clinical practice.
ECOPHYSIOLOGICAL AND ANATOMICAL CHARACTERISTICS OF THE SUBTROPICAL SHRUB ZANTHOXYLUM ACANTHOPODIUM (RUTACEAE) IN CONDITIONS OF A TEMPERATE CONTINENTAL CLIMATE (SERBIA)

The evergreen shrub Zanthoxylum acanthopodium DC. (Rutaceae), originating from warm temperate and subtropical Asia, has existed successfully in the Jevremovac Botanical Garden in Belgrade for more than 80 years. The seasonal pattern of water management in leaves, electrolyte leakage, essential oil composition, and leaf anatomy were examined in order to understand the resistance and viability of this subtropical shrub in the temperate continental cli- mate of Belgrade, Serbia.
Growth Potential of Listeria monocytogenes in Three Different Salmon Products

Cold smoked salmon and sushi salmon have been implicated in outbreaks of listeriosis. We performed challenge tests and a durability study with Listeria monocytogenes on different salmon products to determine the growth potential of this important food-borne pathogen. Data from the challenge test showed a significant growth potential of L. monocytogenes on all of the tested salmon products, with faster growth in sushi salmon than in cold smoked salmon. In identical products that were naturally contaminated at low levels, the durability study did not confirm a high growth potential, possibly due to interactions with competing microflora. The injection of sodium lactate (NaL) at a high concentration (30%) into cold smoked salmon significantly reduced the growth potential of L. monocytogenes. In addition to good manufacturing practices, the injection of higher concentrations of NaL may therefore be a useful additional hurdle to prevent growth of L. monocytogenes to high numbers in the tested salmon products.
Antioxidant Status and Liver Function of Young Turkeys Receiving a Diet with Full-Fat Insect Meal from Hermetia illucens

Simple Summary Insects can be used as an alternative source of protein and fat in poultry feed. To date, in most research, the administration of invertebrates as a replacement for soybean meal in chicken diets has produced satisfactory results. We hypothesized that full-fat insect meal from Hermetia illucens (HI) larvae can also be an acceptable source of protein and energy in the diet of young turkeys, and at the same time can improve their antioxidant status and metabolism. Our research showed that the level of HI meal in the diet of turkeys should not exceed 5%. The use of a higher level of HI than 5% in the diet of young turkeys has a negative effect on lipid metabolism, lipid oxidation and fat deposition in the liver. Abstract We hypothesized that full-fat insect meal from Hermetia illucens (HI) larvae can be an acceptable source of protein and energy in the diet of young turkeys, in an amount adapted to the nutritional needs of these birds, and at the same time can improve their antioxidant status and metabolism. The turkeys were fed a control diet (HI0) without the insect meal, and three diets with increasing HI content of 5%, 10% and 15% (treatments HI5, HI10 and HI15, respectively). The use of 10% or 15% HI in the diet of young turkeys, while beneficially raising levels of P, Fe and Hb, has a negative effect on lipid metabolism, increasing TC levels, lipid oxidation, and fat deposition in the liver. The inclusion of 5% HI in the diet of young turkeys has no adverse effect on the lipid status and histology of the liver, but it does not improve antioxidant status. To conclude, the level of HI meal in the diet of turkeys should not exceed 5%. However, as similar studies on turkeys have not yet been published, overly general conclusions should not be drawn from the results of the present study, and further research is necessary.
Dual Trajectories of Dementia and Social Support in the Mexican-Origin Population.

BACKGROUND AND OBJECTIVES In the next few decades, the number of Mexican American older adults with Alzheimer's Disease and Related Disorders (ADRD) will increase dramatically. Given that this population underutilizes formal care services, the degree of care responsibilities in Mexican American families is likely to increase at the same time. However, little is known about the changing need for assistance with instrumental day-to-day activities and emotional support by long-term patterns of cognitive impairment. RESEARCH DESIGN AND METHODS We use seven waves of the Hispanic EPESE (1992/1993-2010/2011) and trajectory modeling to describe long-term patterns of perceived emotional and instrumental support, and dementia. RESULTS Results revealed two latent classes of both emotional and instrumental support trajectories: low and high support. Specifically, those living alone were more likely to belong to the group with low support than to that with high support. Three latent classes for likely dementia were also revealed: likely dementia, increasing impairment, and no impairment. Those living alone were more likely to belong to the increasing impairment and likely dementia groups. The dual trajectory of emotional and instrumental support with likely dementia revealed that the probability of belonging to the low support group was highest for those with increasing impairment. DISCUSSION AND IMPLICATIONS These findings highlight the risk and vulnerability of those who live alone concerning perceived social support and dementia. Implications of the findings for the potential dependency burden on Latino caregivers are discussed.