Miracle-dz/UniFAR-FTUs huggingface dataset:
Oxygen permeation of various archetypes of oxygen membranes based on BSCF

Ba0.5Sr0. 5Co0.8Fe0. 2O3-δ tubes, capillaries, capillary modules, and asymmetric membranes were prepared and tested for oxygen permeation in a dead-end vacuum operation mode at temperatures up to 850°C. The capillary module was built up by reactive air brazing using seven capillaries and a supply tube. Two machined discs were used as an end cap and as a connector plate. The oxygen permeation behaves according to Wagner at small driving forces, but significant negative deviations were observed for asymmetric membranes and single capillaries at higher ones. This is caused by pressure drops at the vacuum side for single capillaries. The highest oxygen flux was revealed for the capillary module with 175.5 mL(STP)/min at a low-vacuum pressure of 0.042 bar at 850°C, but the asymmetric membrane showing a little bit higher flux at moderate vacuum pressures above 0.07 bar. © 2012 American Institute of Chemical Engineers AIChE J, 58: 3195–3202, 2012
κ Opioid Receptor-Dynorphin Signaling in the Central Amygdala Regulates Conditioned Threat Discrimination and Anxiety

Abstract Neuropeptides within the central nucleus of the amygdala (CeA) potently modulate neuronal excitability and have been shown to regulate conditioned threat discrimination and anxiety. Here, we investigated the role of κ opioid receptor (KOR) and its endogenous ligand dynorphin in the CeA for regulation of conditioned threat discrimination and anxiety-like behavior in mice. We demonstrate that reduced KOR expression through genetic inactivation of the KOR encoding gene, Oprk1, in the CeA results in increased anxiety-like behavior and impaired conditioned threat discrimination. In contrast, reduction of dynorphin through genetic inactivation of the dynorphin encoding gene, Pdyn, in the CeA has no effect on anxiety or conditioned threat discrimination. However, inactivation of Pdyn from multiple sources, intrinsic and extrinsic to the CeA phenocopies Oprk1 inactivation. These findings suggest that dynorphin inputs to the CeA signal through KOR to promote threat discrimination and dampen anxiety.
A 12-Week, Multicenter, Randomized, Double-Blind, Placebo-Controlled Clinical Trial for Evaluation of the Efficacy and Safety of DKB114 on Reduction of Uric Acid in Serum

This study sought to investigate the antihyperuricemia efficacy and safety of DKB114 (a mixture of Chrysanthemum indicum Linn flower extract and Cinnamomum cassia extract) to evaluate its potential as a dietary supplement ingredient. This clinical trial was a randomized, 12-week, double-blind, placebo-controlled study. A total of 80 subjects (40 subjects with an intake of DKB114 and 40 subjects with that of placebo) who had asymptomatic hyperuricemia (7.0–9.0 mg/dL with serum uric acid) was randomly assigned. No significant difference between the DKB114 and placebo groups was observed in the amount of uric acid in serum after six weeks of intake. However, after 12 weeks of intake, the uric acid level in serum of subjects in the DKB114 group decreased by 0.58 ± 0.86 mg/dL and was 7.37 ± 0.92 mg/dL, whereas that in the placebo group decreased by 0.02 ± 0.93 mg/dL and was 7.67 ± 0.89 mg/dL, a significant difference (p = 0.0229). In the analysis of C-reactive protein (CRP) change, after 12 weeks of administration, the DKB114 group showed an increase of 0.05 ± 0.27 mg/dL (p = 0.3187), while the placebo group showed an increase of 0.10 ± 0.21 mg/dL (p = 0.0324), a statistically significant difference (p = 0.0443). In the analysis of amount of change in apoprotein B, after 12 weeks of administration, the DKB114 group decreased by 4.75 ± 16.69 mg/dL (p = 0.1175), and the placebo group increased by 3.13 ± 12.64 mg/dL (p = 0.2187), a statistically significant difference between the administration groups (p = 0.0189). In the clinical pathology test, vital signs and weight measurement, and electrocardiogram test conducted for safety evaluation, no clinically significant difference was found between the ingestion groups, confirming the safety of DKB114. Therefore, it may have potential as a treatment for hyperuricemia and gout. We suggest that DKB114 as a beneficial and safe food ingredient for individuals with high serum uric acid. Trial registration (CRIS.NIH. go. Kr): KCT0002840.
Vapor isotopic evidence for the worsening of winter air quality by anthropogenic combustion-derived water

Significance Water vapor emitted from anthropogenic combustion for winter heating in northern China may exacerbate air pollution. This hypothesis is of considerable scientific and environmental interest. We conducted a multiyear sampling campaign of air vapor isotope compositions and associated atmospheric data from the city of Xi’an, located in an enclosed basin in northwestern China. We found that the fraction of combustion-derived water vapor increases with increasing relative humidity and with the concentration of particulate matter with an aerodynamic diameter less than 2.5 μm in polluted conditions based on field observation, isotopic analysis, and numerical simulation. Our results demonstrated that combustion-derived water is nontrivial when considering energy policy for improving air quality. Anthropogenic combustion-derived water (CDW) may accumulate in an airshed due to stagnant air, which may further enhance the formation of secondary aerosols and worsen air quality. Here we collected three-winter-season, hourly resolution, water-vapor stable H and O isotope compositions together with atmospheric physical and chemical data from the city of Xi’an, located in the Guanzhong Basin (GZB) in northwestern China, to elucidate the role of CDW in particulate pollution. Based on our experimentally determined water vapor isotope composition of the CDW for individual and weighted fuels in the basin, we found that CDW constitutes 6.2% of the atmospheric moisture on average and its fraction is positively correlated with [PM2.5] (concentration of particulate matter with an aerodynamic diameter less than 2.5 μm) as well as relative humidity during the periods of rising [PM2.5]. Our modeling results showed that CDW added additional average 4.6 μg m−3 PM2.5 during severely polluted conditions in the GZB, which corresponded to an average 5.1% of local anthropogenic [PM2.5] (average at ∼91.0 μg m−3). Our result is consistent with the proposed positive feedback between the relative humidity and a moisture sensitive air-pollution condition, alerting to the nontrivial role of CDW when considering change of energy structure such as a massive coal-to-gas switch in household heating in winter.
Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro

Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.
The within-host evolution of antimicrobial resistance in Mycobacterium tuberculosis

ABSTRACT Tuberculosis (TB) has been responsible for the greatest number of human deaths due to an infectious disease in general, and due to antimicrobial resistance (AMR) in particular. The etiological agents of human TB are a closely-related group of human-adapted bacteria that belong to the Mycobacterium tuberculosis complex (MTBC). Understanding how MTBC populations evolve within-host may allow for improved TB treatment and control strategies. In this review, we highlight recent works that have shed light on how AMR evolves in MTBC populations within individual patients. We discuss the role of heteroresistance in AMR evolution, and review the bacterial, patient and environmental factors that likely modulate the magnitude of heteroresistance within-host. We further highlight recent works on the dynamics of MTBC genetic diversity within-host, and discuss how spatial substructures in patients’ lungs, spatiotemporal heterogeneity in antimicrobial concentrations and phenotypic drug tolerance likely modulates the dynamics of MTBC genetic diversity in patients during treatment. We note the general characteristics that are shared between how the MTBC and other bacterial pathogens evolve in humans, and highlight the characteristics unique to the MTBC.
Many Different LINE-1 Retroelements Are Activated in Bladder Cancer

Human genomes contain about 100,000 LINE-1 (L1) retroelements, of which more than 100 are intact. L1s are normally tightly controlled by epigenetic mechanisms, which often fail in cancer. In bladder urothelial carcinoma (UC), particularly, L1s become DNA-hypomethylated, expressed and contribute to genomic instability and tumor growth. It is, however, unknown which individual L1s are activated. Following RNA-immunoprecipitation with a L1-specific antibody, third generation nanopore sequencing detected transcripts of 90 individual elements in the VM-Cub-1 UC line with high overall L1 expression. In total, 10 L1s accounted for >60% of the reads. Analysis of five specific L1s by RT-qPCR revealed generally increased expression in UC tissues and cell lines over normal controls, but variable expression among tumor cell lines from bladder, prostate and testicular cancer. Chromatin immunoprecipitation demonstrated active histone marks at L1 sequences with increased expression in VM-Cub-1, but not in a different UC cell line with low L1 expression. We conclude that many L1 elements are epigenetically activated in bladder cancer in a varied pattern. Our findings indicate that expression of individual L1s is highly heterogeneous between and among cancer types.
Bis-Tridendate Ir(III) Polymer-Metallocomplexes: Hybrid, Main-Chain Polymer Phosphors for Orange–Red Light Emission

In this work, hybrid polymeric bis-tridentate iridium(III) complexes bearing derivatives of terpyridine (tpy) and 2,6-di(phenyl) pyridine as ligands were successfully synthesized and evaluated as red-light emitters. At first, the synthesis of small molecular bis-tridendate Ir(III) complexes bearing alkoxy-, methyl-, or hydroxy-functionalized terpyridines and a dihydroxyphenyl-pyridine moiety was accomplished. Molecular complexes bearing two polymerizable end-hydroxyl groups and methyl- or alkoxy-decorated terpyridines were copolymerized with difluorodiphenyl-sulphone under high temperature polyetherification conditions. Alternatively, the post-polymerization complexation of the terpyridine-iridium(III) monocomplexes onto the biphenyl-pyridine main chain homopolymer was explored. Both cases afforded solution-processable metallocomplex-polymers possessing the advantages of phosphorescent emitters in addition to high molecular weights and excellent film-forming ability via solution casting. The structural, optical, and electrochemical properties of the monomeric and polymeric heteroleptic iridium complexes were thoroughly investigated. The polymeric metallocomplexes were found to emit in the orange–red region (550–600 nm) with appropriate HOMO and LUMO levels to be used in conjunction with blue-emitting hosts. By varying the metal loading on the polymeric backbone, the emitter’s specific emission maxima could be successfully tuned.
A new knockdown resistance (kdr) mutation F1534L in Aedes aegypti associated with insecticide resistance

The control of Aedes aegypti borne-infections mainly dengue, chikungunya, yellow fever and Zika virus relies mainly on vector control measures in the absence of specific drugs or vaccines available against these infections. Emergence of insecticide resistance in Ae. aegypti may pose serious threat to the success of insecticide-based vector control programme. Here, we report the presence of multiple knockdown resistance (kdr) mutations present in an Indian Ae. aegypti population including a new mutation F1534L (not reported earlier in Ae. aegypti) which is associated with DDT and pyrethroid resistance. DNA sequencing of partial domain II, III and IV of the voltage gated sodium channel (VGSC) performed on Ae. aegypti collected from Bengaluru, India, revealed the presence of four kdr mutations, i.e., V1016G and S989P in domain II and two alternative kdr mutations F1534C and F1534L in domain III. Allele specific PCR assays (ASPCR) were developed for the detection of kdr mutations V1016G and S989P while a PCR-RFLP based strategy was adopted for the genotyping of all three known mutations in domain III (F1534L, F1534C and T1520I). Genotyping of 572 Ae. aegypti samples collected in 2014 and 2015 revealed a moderate frequency of V1016G/S989P (18.27%) and F1534L (17.48%), a relatively high frequency of F1534C (50.61%) and absence of T1520I in the population. Mutations V1016G and S989P were in complete linkage disequilibrium while they were having negative linkage disequilibrium with kdr alleles F1534C and F1534L. The new mutation F1534L showed significant protection against permethrin, deltamethrin and DDT whereas F1534C showed protection against permethrin and DDT but not against deltamethrin.
Underpotential Deposition of Copper on Gold and the Effects of Thiourea Studied by AC Impedance

This paper reports ac impedance and cyclic voltammetry studies of underpotential deposition (UPD) of Cu on Au in an electrolyte composed of CuSO/sub 4/ and H/sub 2/SO/sub 4/. A theoretical kinetic model was developed which fits the experimental impedance spectra over frequencies ranging from 0.001 to 20,000 Hz. Spectra are characterized by a two-time constant process; two capacitive loops were observed in the Nyquist diagrams corresponding to two distinctive peaks in the Bode phase plot. The high frequency time constant is associated with the double-layer capacitance, while the low frequency process is due to relaxation phenomena associated with the charge transfer involved in Cu/sup 2/ electrosorption. Thiourea is one of the most important organic additives for commercial CuSO/sub 4/ plating baths. Therefore, the coadsorption of thiourea and UPD Cu on Au was also studied by ac impedance. Adsorbed thiourea was found to have a dramatic effect on the high frequency peak in the Bode phase plot. Measurements such as these are proposed as a means of determining organic additive concentrations in Cu plating and electroforming baths.
Charge Carrier Transport Mechanism in Amorphous Layers of the Composition Ge4Se5Te

The non-linear current-voltage behaviour of amorphous thin films of the composition Ge4Se5Te is studied in dependence on the temperature and on the thickness of the layers. The current-voltage characteristics are interpreted in terms of the three-dimensional Poole-Frenkel theory for single centres. Other mechanism could be excluded. An exact way for analysing the curves is shown relating the phenomenological Poole-Frenkel theory with the model of Onsager for electric-field-enhanced dissociation of weak electrolytes deduced from statistical thermodynamics. Es wird uber das nichtlineare Strom–Spannungsverhalten dunner amorpher Schichten der Zusammensetzung Ge4Se5Te in Abhangigkeit von Temperatur und Schichtdicke berichtet. Die Kennlinien sind im dreidimensionalen Modell des Poole-Frenkel-Effekts interpretierbar. Andere Mechanismen konnen ausgeschlossen werden. Es wird ein exakter Weg zur Auswertung der Kurven aufgezeigt, der sich aus der Aquivalenz der phanomenologischen Beschreibung des Poole-Frenkel-Effekts und der aus der statistischen Thermodynamik abgeleiteten Theorie der feldinduzierten Dissoziation schwacher Elektrolyte von Onsager ergibt.
SugarPy facilitates the universal, discovery-driven analysis of intact glycopeptides

Motivation Protein glycosylation is a complex post-translational modification with crucial cellular functions in all domains of life. Currently, large-scale glycoproteomics approaches rely on glycan database dependent algorithms and are thus unsuitable for discovery-driven analyses of glycoproteomes. Results Therefore, we devised SugarPy, a glycan database independent Python module, and validated it on the glycoproteome of human breast milk. We further demonstrated its applicability by analyzing glycoproteomes with uncommon glycans stemming from the green alga Chlamydomonas reinhardtii and the archaeon Haloferax volcanii. SugarPy also facilitated the novel characterization of glycoproteins from the red alga Cyanidioschyzon merolae. Availability The source code is freely available on GitHub (https://github.com/SugarPy/SugarPy), and its implementation in Python ensures support for all operating systems. Contact mhippler@uni-muenster.de and pohlschr@uni-muenster.de Supplementary information Supplementary data are available online.
Interferon Beta Activity Is Modulated via Binding of Specific S100 Proteins

Interferon-β (IFN-β) is a pleiotropic cytokine used for therapy of multiple sclerosis, which is also effective in suppression of viral and bacterial infections and cancer. Recently, we reported a highly specific interaction between IFN-β and S100P lowering IFN-β cytotoxicity to cancer cells (Int J Biol Macromol. 2020; 143: 633–639). S100P is a member of large family of multifunctional Ca2+-binding proteins with cytokine-like activities. To probe selectivity of IFN-β—S100 interaction with respect to S100 proteins, we used surface plasmon resonance spectroscopy, chemical crosslinking, and crystal violet assay. Among the thirteen S100 proteins studied S100A1, S100A4, and S100A6 proteins exhibit strictly Ca2+-dependent binding to IFN-β with equilibrium dissociation constants, Kd, of 0.04–1.5 µM for their Ca2+-bound homodimeric forms. Calcium depletion abolishes the S100—IFN-β interactions. Monomerization of S100A1/A4/A6 decreases Kd values down to 0.11–1.0 nM. Interferon-α is unable of binding to the S100 proteins studied. S100A1/A4 proteins inhibit IFN-β-induced suppression of MCF-7 cells viability. The revealed direct influence of specific S100 proteins on IFN-β activity uncovers a novel regulatory role of particular S100 proteins, and opens up novel approaches to enhancement of therapeutic efficacy of IFN-β.
Promoting Reminiscences with Virtual Reality Headsets: A Pilot Study with People with Dementia

This study aimed to explore the feasibility and effects of promoting reminiscences, using virtual reality (VR) headsets for viewing 360° videos with personal relevance, with people with dementia. A study with a mixed methods design was conducted with nine older adults diagnosed with dementia. Interventions consisted of four sessions, in which the participants’ engagement, psychological and behavioral symptoms, and simulation sickness symptoms were evaluated. Neuropsychiatric symptomatology and quality of life were measured pre- and post-intervention. Caregivers were interviewed regarding the effect of the approach. In most cases, participants appeared to enjoy the sessions, actively explored the 360° environment, and shared memories associated with the depicted locations, often spontaneously. There were no cases of significant increases in simulator sickness and psychological and behavioral symptoms during sessions, with only some instances of minor eyestrain, fullness of head, anxiety, irritability, and agitation being detected. Although there were no significant changes in the measured outcomes after intervention, the caregivers assessed the experience as potentially beneficial for most participants. In this study, promoting reminiscences with VR headsets was found to be a safe and engaging experience for people with dementia. However, future studies are required to better understand the added value of immersion, using VR, in reminiscence therapy.
Effect of Geographical Indication Information on Consumer Acceptability of Cooked Aromatic Rice

Geographical indication (GI) labeling is used to represent information about specific geographical origins of target products. This study aimed at determining the impact of GI information on sensory perception and acceptance of cooked aromatic rice samples. Ninety-nine participants evaluated cooked rice samples prepared using each of three aromatic rice varieties both with and without being provided with GI information. Participants rated the acceptance and intensity of the cooked rice samples in terms of appearance, aroma, flavor, texture, and overall liking, and also reported how important the GI information was to them. The results showed that consumers rated the cooked rice samples higher in appearance and overall liking when provided with GI information. Interestingly, participants who valued “state-of-origin” information more highly exhibited increased hedonic ratings of cooked rice samples when provided with GI information, but not when no GI information was given. Participants provided with GI information rated flavor or sweetness intensities of cooked aromatic rice samples closer to just-about-right than those without such information. This study provides empirical evidence about how GI information modulates sensory perception and acceptance of cooked aromatic rice samples. The findings will help rice industry, farmers, and traders better employ GI labeling to increase consumer acceptability of their rice products.
The N2N3 domains of ClfA, FnbpA, and FnbpB in Staphylococcus aureus bind to human complement factor H, and their antibodies enhance the bactericidal capability of human blood.

In the complement system, the opsonin C3b binds to the bacterial cell surface and mediates the opsonophagocytosis. However, the cell wall protein SdrE of Staphylococcus aureus inhibits the C3b activity by recruiting the complement regulatory protein factor H (fH). SdrE binds to fH via its N-terminal N2N3 domain, which are also found in six other staphylococcal cell wall proteins. In this study, we report that not only the N2N3 domain of SdrE but also those of ClfA, FnbpA, and FnbpB can bind to fH. When immobilized on a microplate, the N2N3 domains recruited fH and enhanced the factor I (fI)-mediated cleavage of C3b. When mixed with fH and S. aureus cells, the N2N3 domains inhibited the fH binding to S. aureus cells and reduced the fI-mediated C3b cleavage on the bacterial cell surface. The F(ab)'2 fragments of the rabbit N2N3 antibodies also inhibited the fH-binding to the S. aureus cell surface. When added to human blood, the N2N3 antibodies or the N2N3 domain proteins significantly increased the bactericidal activity. Based on these results, we conclude that, in S. aureus, not only SdrE but also ClfA, FnbpA, and FnbpB can contribute to the inhibition of C3b-mediated opsonophagocytosis.