Miracle-dz/UniFAR-FTUs huggingface dataset:
Psychosocial Support in Liver Transplantation: A Dyadic Study With Patients and Their Family Caregivers

Background and aims: Liver transplantation provides an opportunity of survival for patients with liver failure; however, this procedure is known to be psychologically and physically fatiguing for patients and their informal caregivers. The aim of this study was to investigate how perceived social support and the distribution of dependency were associated with the psychological wellbeing of patients waiting for liver transplantation and their caregivers, as a dyad. Methods: The present was a cross-sectional study. Ninety-five participants were recruited at a hospital in Northern Italy, during the psychological evaluation for inclusion in the transplantation list: 51 patients (19 with alcohol-related illness) and 44 family caregivers. Both patients and caregivers filled in a Symptom Checklist and Kelly’s Dependency Grids. Patients also compiled the Medical Outcome Study Social-Support Survey, and caregivers compiled the Family Strain Questionnaire Short-Form. Results: Caregivers reported important levels of strain and strongly related to a worsening of their own and patients’ symptoms. Patients with alcohol-related pathologies had a narrower social network, which corresponded to an increase in family strain. On the sample as a whole, regression analyses showed that perceived social support and dependency measures did not predict patients’ and caregivers’ symptoms. Nevertheless, cluster analysis identified a group of caregivers who distributed their dependency more and experienced lower levels of depression, anxiety, and strain. Conclusions: These results suggest the usefulness of a dyadic approach in the research, prevention, and care of liver diseases. A deeper comprehension of the functioning of dyads will help practitioners in the identification of situations at risk.
Penumbra width determination of single beam and 201 beams of Gamma Knife machine model 4C using Monte Carlo simulation

Abstract Background One of the stereotactic radiosurgery techniques is Gamma Knife radiosurgery, in which intracranial lesions that are inaccessible or inappropriate for surgery are treated using 201 cobalt-60 sources in one treatment session. In this conformal technique, the penumbra width, which results in out-of-field dose in tumour-adjacent normal tissues should be determined accurately. The aim of this study is to calculate the penumbra widths of single and 201 beams for different collimator sizes of Gamma Knife machine model 4C using EGSnrc/BEAMnrc Monte Carlo simulation code and comparison the results with EBT3 film dosimetry data. Methods and materials In this study, simulation of Gamma Knife machine model 4C was performed based on the Monte Carlo codes of EGSnrc/BEAMnrc. To investigate the physical penumbra width (80−20%), the single beam and 201 beams profiles were obtained using EGSnrc/DOSXYZnrc code and EBT3 films located at isocentre point in a spherical Plexiglas head phantom. Results Based on the results, the single beam penumbra widths obtained from simulation data for 4, 8, 14 and 18 mm collimator sizes along X axis were 0·75, 0·77, 0·90 and 0·92 mm, respectively. The data for 201 beams obtained from simulation were 2·61, 4·80, 7·92 and 9·81 mm along X axis and 1·31, 1·60, 1·91 and 2·14 mm along Z axis and from film dosimetry were 3·21, 4·90, 8·00 and 10·61 mm along X axis and 1·22, 1·69, 2·01 and 2·25 mm along Z axis, respectively. Conclusion The differences between measured and simulated penumbra widths are in an acceptable range. However, for more precise measurement in the penumbra region in which dose gradient is high, Monte Carlo simulation is recommended.
Production potential and economic feasibility of sesame (Sesamum indicum) - based intercropping with legumes under rainfed conditions

A field experiment was conducted during rainy season of 2002 and 2003 under rainfed conditions at Oilseeds Research Station, Kangra to find out the most appropriate sesame based intercropping system and their planting pattern for higher productivity and returns. The sesame yield decreased with soybean and blackgram intercropping in all planting pattern by 49.6-69.1 and 41.7-64.7 % respectively. Sole soybean gave the highest sesanle equivalent yield (0.715 tonnes/ha) and net returns (Rs 16 010), whereas sesanle sole the lowest yield (0.456 tonne/ha) and returns (Rs 8 792). Intercropping in sesanle with soybean and blackgran1 increased its productivity by 22.8-32.7% over the sole sesame cropping. Both the sesame and intercrops in the system were equally competitive when broadcast and 1:1 planting pattern, whereas paired row planting of intercrop dominated the sesame crop. Among intercropping systems sesame +blackgram (1:1) gave the maximum land equivalent ratio (1.17), sesame equivalent yield (0.605 tonne/ha) and net return (Rs 13 510).
A full-term infant with type II thanatophoric dysplasia

Abstract Objectives To report a neonate with clinical findings consistent with thanatophoric dysplasia (TD). Only a few cases of this rare and lethal skeletal disorder have been reported in South-East Asia. Case presentation A 37-year-old Asian female, fourth gravida at 39 weeks, presented to our hospital for an elective cesarean section due to polyhydramnios, frank breech and gestational hypertension. The father was a 42-year-old Asian male. There was no history of rashes, fever, alcohol intake, substance drug abuse, smoking habit or radiation exposure. Ultrasound (US) of 34-weeks’ gestation found a baby with frontal bossing, prominent temporal lobe, clover-skull and low nasal bridge. The thoracic diameter was smaller than the abdominal diameter. Short limbs without bowing were noted in femur and humeral bones. The patient delivered a baby boy, 4115 g, APGAR scores were 5 and 7 at 1 and 5 min. The baby had a dysmorphic face, frontal bossing, low nasal bridge, low-set ears and short neck. The thorax was narrow and abdomen was protuberant. The upper and lower proximal limbs appeared short. A chest X-ray revealed short, curved ribs and opacification of both lungs. He had respiratory distress shortly after birth and had persistent severe respiratory distress despite adequate mechanical ventilation. On the third day, he had cardiac arrest; resuscitation was not done due to family request. The baby was declared deceased due to cardiopulmonary failure related to his congenital anomaly. Conclusions Ultrasonography could readily indicate TD prenatally. The pregnancy can continue up to late third trimester without miscarriage. Most of the neonates die in utero; those who survive are dependent on ventilator.
The Involvement of Calcium in Heat-induced Coral Bleaching

89 low temperatures. A similar phenomenon was not observed in experiments with several scleractinian corals (Steen and Muscatine 1987). Muscatine et al. (1991) suggested that release of symbiotic algae from corals could involve thermotropic effects on host cell membranes after cold shock. Fang et al. (1997) found that the concentration of intracellular calcium in coral cells increased following exposure to elevated temperatures. It was demonstrated that the increase of intracellular calcium appeared to trigger the exocytosis of cortical granules from fertilized oocytes (for review, see Knight et al. 1989) and synaptic vesicles from synaptic terminals (Heidelberger et al. 1994). Therefore, the increasing calcium may act as a signal that plays an important role in the mechanism of coral bleaching, such as exocytosis of intracellular vacuoles which contain the zooxanthellae. In experiments described here, we measured the change in concentration of intracellular calcium after cells were subjected to elevated temperatures. Heat-induced bleaching in normal samples was compared with bleaching in samples in which intracellular calcium had been depleted by treatThe Involvement of Calcium in Heat-induced Coral Bleaching Shiao-Ping Huang, Ku-Lin Lin and Lee-Shing Fang* Institute of Marine Resources, National Sun Yat-sen University, Kaohsiung, Taiwan 804, R.O. C. National Museum of Marine Biology and Aquarium, Preparatory Office, Kaohsiung, Taiwan 800, R.O. C. Department of Medical Technology, Foo-Yin Institute of Technology, Kaohsiung, Taiwan 831, R.O. C.
Efficacy Profile of the Homeopathic Combination for Influenza and Acute Respiratory Viral Diseases Treatment and Prevention

Background. Acute respiratory viral infection (ARVI) is accompanied by a general intoxication syndrome and respiratory mucosa predominant damage. A significant number of viral nature pathogens from various nosological groups (influenza viruses, parainfluenza, adenoviruses, respiratory syncytial viruses, coronaviruses, picornaviruses, rhinoviruses, enteroviruses, herpesviruses, etc.) could cause development of ARVI at human respiratory tract. Methods of ethnoscience and non-traditional medicine can be used as part of integrated treatment and prevention of influenza and ARVI. With the help of anti-cold homeopathic remedies, people can be treated from the very beginning of the disease when an elevated temperature is observed and there are all signs of intoxication and inflammation. Objective. Scientific justification for the safety and efficacy of a complex homeopathic formulation in tablet form ( Aconitum napellus D6, Ammonium bromatum D4, Atropa belladonna D6, Bryonia D6, Cinchona pubescens D6, Echinacea D3, Hydrargyrum bicyanatum D8, Rhustoxicodendron D6) intended to enhance the natural immunity in influenza and respiratory diseases, to normalize the functional state of the immune system and upper respiratory tract. Results. Most patients have acute febrile respiratory syndrome, and some population groups have an increased risk of complications in the form of severe illness or death. These groups include elderly people, very young people, and people with concomitant illnesses. Annually, every third inhabitant of the planet suffers from acute respiratory infections. We have conducted a scientific substantiation of the safety and efficacy of a complex homeopathic tablet formulation ( Aconitum napellus D6, Ammonium bromatum D4, Atropa belladonna D6, Bryonia D6, Cinchona pubescens D6, Echinacea D3, Hydrargyrum bicyanatum D8, Rhustoxico ­dendron D6), which can be used to treat and prevention of these diseases. Conclusions. Components of developed homeopathic preparation are used in medical practice as anti-cold and immunomodulating mono- and complex homeopathic preparations for more than 100 years. There are no restrictions on the use of the drug in the context of its safety profile, since the active ingredient concentrations used are completely non-toxic. The preparation enhances the body’s protective responses and promotes the relief of symptoms of acute respiratory infections and flu (headache, sneezing, runny nose, sore throat, body aches, fever) and rapid recovery.
Molecular analysis of the telomeric half of human chromosome 2q

The first part of my thesis dealt with the physical mapping of human chromosome 2 employing the yeast artificial chromosome (YAC) cloning system. To generate a chromosome 2 YAC sublibrary, over 1,000 interspersed repetitive sequence (IRS)-PCR probes were generated and used to screen the CEPH midi YAC library and approximately 2,000 chromosome 2-specific midi YACs were identified. These YACs were divided into 223 YAC groups, i.e., sets of unordered overlapping YACs, and using publicly available contig analysis software, a tentative order of YACs within each YAC group could be established. To order YAC groups, the chromosome 2 YAC sublibrary was screened with 87 genetically mapped microsatellites and cytogenetically mapped expressed sequence tags (ESTs), and 44 YAC groups were localized along the genetic map of chromosome 2q. In addition, 16 known genes were physically linked with microsatellites within YAC groups, thus providing integration points for genetic, cytogenetic and YAC-based physical maps of chromosome 2q. In a subsequent step of the analysis, the chromosome 2 YAC mapping data created by the Whitehead Institute (WI)/MIT Genome Center were integrated into our dataset. The integrated dataset consisted of 240 YAC groups, of which 14 large groups containing both our and WL/MIT Genome Center YAC groups were located on chromosome 2q. These 14 groups consisted of 1,195 YACs, which will form the backbone for the construction of a complete YAC contig for human chromosome 2q.
Cloning and Characterization of thegskGene Encoding Guanosine Kinase ofEscherichia coli

The Escherichia coli gsk gene encoding guanosine kinase was cloned from the Kohara gene library by complementation of the E. coli gsk-1 mutant allele. The cloned DNA fragment was sequenced and shown to encode a putative polypeptide of 433 amino acids with a molecular mass of 48,113 Da. Minicell analysis establishedthesubunitMras43,500.PrimerextensionanalysisindicatedthepresenceofanadequatePribnow box and suggested that the transcript contained a 110-base leader sequence. Strains harboring thegskgene on multicopy plasmids overexpressed both guanosine and inosine kinase activities. N-terminal sequence and amino acid composition analyses of the 43,500-Mr polypeptide band confirmed the correct reading frame assignment and the identity of this band as thegskgene product. Comparison of the amino acid sequence with the protein database revealed similarity to regions of other mononucleotide-utilizing enzymes. Eukaryotic and prokaryotic cells in which ribonucleoside kinase activity has been investigated usually contain two enzymatic activities, one specific for adenosine and the other specific for uridine and cytidine. Escherichia coli and Salmonella typhimurium are exceptions to this because they contain guanosine-inosine kinase (ATP:guanosine 59-phosphotransferase [EC 2.7.1.73]) instead of adenosine kinase (24). Guanosinespecific ribonucleoside kinase activity has been demonstrated with certainty in only a few organisms, including E. coli, S. typhimurium (24), and Trichomonas vaginalis (20). It is also found in the mitochondria of plants and mammals (8, 33). The E. coli enzyme is encoded by the gsk gene, which has been mapped to 11 min on the linkage map (2, 14). The utilization of exogenously supplied or endogenously synthesized guanosine and inosine for nucleotide biosynthesis inE.coliandS. typhimuriumoccursthroughtwopathways.The majorpathwayinvolvesphosphorolysisofguanosineorinosine to the corresponding nucleobase and ribose 1-phosphate. This reaction is catalyzed by purine nucleoside phosphorylase, which is encoded in E. coli by the deoD gene. Guanine and hypoxanthine are subsequently phosphoribosylated to their correspondingnucleosidemonophosphatesbyguanineandhypoxanthine phosphoribosyltransferases (gpt and hpt, respectively). The minor pathway involves direct phosphorylation of guanosine or inosine to the corresponding nucleoside 59monophosphate solely through the action of guanosine kinase. The guanosine-inosine kinase pathway can be studied unambiguously in vivo only indeoDmutant strains where the functionofguanosinekinasehasbeendemonstratedtobeessential for guanosine salvage (11, 14, 15). Methods. The E. coli K-12 strains used in this study are shown in Table 1. The growth medium was either NZY broth (13) or AB minimal medium (7) supplemented with glucose (0.2%) and thiamine (0.5 mg/liter). When necessary, methionine or histidine was added to 40 mg/liter. Hypoxanthine, adenine, or guanine was added to 15 mg/liter, and guanosine was addedto30mg/liter.Ampicillinwasusedataconcentrationof
Running head: Phosphate translocators in red algae

Abstract In chloroplasts of green plants and algae, CO 2 is assimilated into triose-phosphates; a large part of these triose-phosphates is exported to the cytosol by a triose-phosphate/phosphate translocator (TPT) whereas some is stored in the plastid as starch. Plastidial phosphate translocators (PTs) have evolved from transport proteins of the host endomembrane system shortly after the origin of chloroplasts by endosymbiosis. The red microalga Galdieria sulphuraria shares three conserved putative orthologous transport proteins with the distantly related seed plants and green algae. However, red algae, in contrast to green plants, store starch in their cytosol, not inside plastids. Hence, due to the lack of a plastidic starch pool, a larger share of recently assimilated CO 2 needs to be exported to the cytosol. We thus hypothesized that red algal transporters have distinct substrate specificity in comparison to their green orthologs. This hypothesis was tested by expression of the red algal genes in yeast and assessment of their substrate specificities and kinetic constants. Indeed, two of the three red algal PT candidate orthologs have clearly distinct substrate specificities when compared to their green homologs. GsTPT displays very narrow substrate specificity and high affinity; in contrast to green plant TPTs, 3-phosphoglyceric acid is poorly transported and thus not able to serve as a triose phosphate/3-PGA redox shuttle
Studies of cultured neuronal networks using light activated ion channels and pumps

In the presented thesis work, an “Optical Network Electrophysiology “ system that combines optical stimulation using optogenetic tools and multisite neuronal recording using microelectrode arrays was developed and its applicability to address questions related to neuronal network dynamics was demonstrated. The system was used to modify the intrinsic collective dynamics of a cultured neuronal network potentially maximizing spike synchronization using mild whole field photostimulation. It offers an attractive alternative to stimulation paradigms that externally control neuronal networks. Another application of the system was to drive neurons in a naturalistic in-vivo like fashion where fluctuating light waveforms where used to put neurons in “fluctuation driven regime”. This regime is crucial to characterize basic computational properties of neurons such as frequency-input curves, spike triggered average and correlation gain.
General Anaesthesia with Muscle Paralysis in Patients with Anterior Mediastinal Mass – an audit of 15 cases and review of literature

General Anaesthesia (GA) with PPV can lead to cardiorespiratory collapse in patients with AMM. However, adequate relaxation facilitates major surgery and it is important to be able to predict when it is safe to use muscle paralysis. The medical notes of patients who had undergone resection of AMM were studied to look for complications developed due to PPV. The clinical features, lung functions, echocardiography and computed tomography (CT) findings were recorded to see if any of them could predict adverse events.
Macintosh Laryngoscope Assisted Flexible Fiberoptic Endotracheal intubation Versus Flexible Fiberoptic Endotracheal Intubation alone for modified Mallampati III & IV Patients: A Prospective Randomized Controlled Study

Background: The intubation difficulty of using the flexible FOB could be due to the inability to visualize the glottis and/or failure to advance and railroad the endotracheal tube. The combined use of Macintosh laryngoscope with the flexible FOB could be a safe technique that facilitate the flexible FOB intubation function in non-relaxed spontaneously breathing patients predicted to be difficult for endotracheal intubation. Methods: A prospective randomized controlled study included 100 patients predicted to be difficult for airway intubation, ASA physical status Ӏ-III, aging 18-65 years. Patients were randomly allocated into two groups 50 patients each, the control group (Group F) in which flexible- video assisted-FOB was used alone and the study group (Group MF) in which combined Macintosh - flexible-video assisted-FOB used for endotracheal intubation under inhalational general anesthesia out of any muscle relaxant use. The study compared the techniques for endotracheal tube insertion time, 1st trial success rate, number of attempts, complications, and hemodynamic changes between both groups. Results: There were significant decrease in endotracheal tube insertion time Group MF (32.1 ± 7.9) compared to Group F (68.6 ± 12.6), significant increase in 1st trial success in Group MF 49 (98%) compared to Group F 43 (86%), with acceptable Intubation conditions and hemodynamics for both groups. Conclusion: The combined use of Macintosh laryngoscope with the flexible FOB in predicted difficult endotracheal intubation conditions provides significant decrease in endotracheal tube insertion time (near 50% reduction), significant increase in 1st endotracheal intubation trial success rate, acceptable intubating conditions and hemodynamic stability compared to flexible FOB alone.
The conditions of possibilities for recovery: A critical discourse analysis in a Danish psychiatric context.

AIMS AND OBJECTIVES This paper explores the conditions for the possibilities of recovery in a Danish mental healthcare practice, expressed from the perspective of nurses. The results and discussion of the study help to make visible and explore the muddle of conceptualisations of recovery in mental healthcare practice. BACKGROUND Few studies examine the possibilities of recovery for inpatients and outpatients in mental health centres from a nursing perspective. DESIGN A qualitative design using a critical social-constructionist frame of understanding, in which the real world is considered as a series of social constructions. METHOD A Fairclough-inspired critical discourse analysis was chosen as the analytical strategy. The analysis is comprised of ten interviews in mental healthcare and notes, written by nurses, in medical records of ten patients with a mental illness admitted to a mental healthcare centre in 2016-2017. The Consolidated Criteria for Reporting Qualitative Research checklist was used as a guideline to secure accurate and complete reporting of the study (See Supplementary File 1). RESULTS From the findings of the textual analysis and the analysis of the discourse practice, it seems clear that the social relations and structures relating to recovery in Danish psychiatry are steered and controlled by discourses that reflect, in general terms, the essence of the core of neoliberal ideology. CONCLUSION Recovery is generally articulated from an overall discourse of neoliberalism with its embedded discourses of paternalism, biomedicine, self-care, and holism. All these discourses coexist in nursing practice, but the paternalistic discourse becomes the framework for the conditions for the possibility of how recovery is expressed in practice. RELEVANCE TO CLINICAL PRACTICE Nurses need to be supported to seek clarity in the understanding and operationalisation of a recovery-oriented approach, if the agenda is to be truly adopted and strengthened.