Miracle-dz/UniFAR-FTUs huggingface dataset:
Sinomenine restrains breast cancer cells proliferation, migration and invasion via modulation of miR-29/PDCD-4 axis

Abstract Sinomenine (Sino) is diffusely applied in heal rheumatoid arthritis and neuralgia. Howbeit, the activities of Sino in breast cancer cells remain confused. The research attempted to probe the anti-tumor function of Sino in breast cancer cells and divulge the feasible molecular mechanism. Sion at the 1–16 μM concentrations was exploited for the exposure of MDA-MB-231 or MCF7 cells, and cell growth, migration, invasion, cell cycle-relevant and apoptosis-correlative factors were estimated. Micro RNA (miR)-29 expression was evaluated via enforcing qRT-PCR, and the actions of miR-29 in MDA-MB-231 cells growth, migration and invasion were appraised after the overexpressed or suppressed vectors transfection. The functions of PDCD-4 in JNK and MEK/ERK pathways were estimated by employing western blot. We found that, Sino exposure impeded cell proliferation, provoked cell apoptosis and barricaded cell migration and invasion in MDA-MB-231 and MCF7 cells. Enhancement of miR-29 was observed in Sino-managed cells, and miR-29 overexpression further potentiated the activities of Sino in MDA-MB-231 cells. Additionally, Sino remarkably enhanced PCDC-4 expression via adjusting miR-29 in MDA-MB-231 cells. Beyond that, overexpressed PCDC-4 obstructed JNK and MEK/ERK pathways in MDA-MB-231 cells. Taken together, the explorations unveiled that Sino restrained MDA-MB-231 cells proliferation, migration, invasion, and provoked apoptosis through modulation of miR-29/PDCD-4 axis. Highlight Sino inhibits MDA-MB-231 and MCF7 cells proliferation and provokes apoptosis; Sino restrains MDA-MB-231 and MCF7 cells migration and invasion; Sino ascends miR-29 expression in MDA-MB-231 and MCF7 cells; Sino adjusts cell growth, migration and invasion via modulating miR-29; Sino up-regulates PDCD-4 expression through mediating miR-29; PDCD-4 obstructs JNK and MEK/ERK pathways in MDA-MB-231 cells.
Parental psychological control and academic functioning in Chinese high school students: A short-term longitudinal study.

The present study aimed to examine the reciprocal relationship between parental psychological control and students' academic functioning in urban China. Participants were 731 Chinese high school students in grade 10 (356 boys; Mage = 15.64 years, SD = 0.68). Two waves of 1-year longitudinal data were collected using student reports of parental psychological control and academic-related beliefs, strategies, and behaviours. Results showed that parental psychological control at Time 1 significantly triggered an increase in students' maladaptive academic functioning at Time 2; and students' adaptive academic functioning at Time 1 significantly predicted parental psychological control at Time 2. Limitations of the present study and implications for practice are discussed. Statement of contribution What is already known on this subject? According to self-determination theory, parental psychological control has been found to be harmful on students' academic learning in Western societies. We know little about the relation between parental psychological control and academic functioning (adaptive vs. maladaptive) in Eastern societies such as China. What does this study add? Parental psychological control increased maladaptive academic functioning, and adaptive academic functioning decreased parental psychological control, suggesting a more fluid, dynamic parenting-child interaction over time. The predicted relations between parental psychological control and academic functioning of high school students hold across gender. More urbanized adolescents had a high tendency to perceive their parents as psychological controlling, suggesting a change in culture regarding the importance of personal autonomy for more urbanized adolescents.
Development of Intelligent Prefabs Using IoT Technology to Improve the Performance of Prefabricated Construction Projects

Prefabrication (PC) projects have many advantages, such as cost and energy savings and waste reduction. However, some problems still exist that hamper the development of prefabrication projects. To improve PC project performance and advance innovation in construction, this study introduces an innovative method that incorporates Radio Frequency Identification (RFID) and Long Range (LoRa) technologies, sensor networks, the BIM model and cloud computing to automatically collect, analyze and display real-time information about PC components. It can locate PC components on a construction site and monitor their structural performance during the installation process. RFID technology and strain sensors were used to collect the required data on a construction site. All the data was transmitted to a server using LoRa technology. Then, the cloud-based Building Information Modelling (BIM) model of the project was developed to store and vividly present project information and real-time onsite data. Moreover, the cloud-based BIM model enables project team members to access the project information from anywhere by using mobile devices. The proposed system was tested on a real PC project to validate its effectiveness. The results indicate that the sensor network can provide reliable data via LoRa technology, and a PC component can be accurately located on site. Also, the monitoring data of structural performance for the PC component during the installation process is acceptable. The proposed method using innovative technologies can improve PC project performance and help industry professionals by providing sufficient required information.
Being an insider and outsider: whiteness as a key dimension of difference

This article demonstrates the significance of engaging with whiteness as a key dimension of difference shaping research in multi-faceted ways. I critically reflect on a research project that included interviews with Muslim men in Rotherham, a northern English town that had experienced a child sexual exploitation crisis involving Pakistani Muslim men. It raised significant methodological and epistemological issues regarding my position in the research, as a white female researcher, and my relationships with local Pakistani Muslim men and women. I highlight the fluidity of my insider–outsider position through exploring political and ethical dilemmas involved in carrying out the research and structural and experiential aspects of researcher subjectivity. I show how being white both facilitated and obstructed the research as I steered my way through a highly sensitive set of circumstances and how engaging with whiteness is key in democratising research and shedding light on unequal power relations in knowledge production.
Force generated by myosin cross-bridges is reduced in myofibrils exposed to ROS/RNS.

Skeletal muscle weakness is associated with oxidative stress and oxidative post-translational modifications on contractile proteins. There is indirect evidence that reactive oxygen/nitrogen species (ROS/RNS) affect skeletal muscle myofibrillar function, although the details of ROS/RNS' acute effects on myosin-actin interactions are not known. In this study, we examined the effects of peroxynitrite (ONOO-) on the contractile properties of individual skeletal muscle myofibrils by monitoring myofibril-induced displacements of an atomic force cantilever upon activation and relaxation. The isometric force decreased by approximately 50% in myofibrils treated with the ONOO-donor (SIN-1) or directly with ONOO-, which was independent of the cross-bridge abundancy condition (i.e. rigor or relaxing condition) during SIN-1 or ONOO-treatment. The force decrease was attributed to an increase in the cross-bridge detachment rate (gapp) in combination with a conservation of the force redevelopment rate (kTr), hence an increase in the population of cross-bridges transitioning from force-generating to non-force-generating cross-bridges during steady-state. Taken together, the results of this study provide important information of how ROS/RNS affect myofibrillar force production which may be of importance for conditions where increased oxidative stress is part of the pathophysiology.
Lichenihabitans psoromatis gen. nov., sp. nov., a member of a novel lineage (Lichenihabitantaceae fam. nov.) within the order of Rhizobiales isolated from Antarctic lichen.

Two Gram-stain-negative, facultative anaerobic chemoheterotrophic, pink-coloured, rod-shaped and non-motile bacterial strains, PAMC 29128 and PAMC 29148T, were isolated from lichen. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strains PAMC 29128 and PAMC 29148T belong to lichen-associated Rhizobiales-1 (LAR1), an uncultured phylogenetic lineage of the order Rhizobiales and the most closely related genera were Methylocapsa (<93.9 %) and Methylosinus (<93.8 %). The results of phylogenomic and genomic relatedness analyses also showed that strains PAMC 29128 and PAMC 29148T were clearly distinguished from other species in the order Rhizobiales with average nucleotide identity values of <71.4 % and genome-to-genome distance values of <22.7 %. Genomic analysis revealed that strains PAMC 29128 and PAMC 29148T did not contain genes involved in atmospheric nitrogen fixation or utilization of carbon compounds such as methane and methanol. Strains PAMC 29128 and PAMC 29148T were able to utilize certain monosaccharides, disaccharides, sugar alcohols and other organic compounds as a sole carbon source. The major fatty acids (>10 %) were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c; 33.7-39.7 %), summed feature 3 (C16 : 1 ω7c and/or C 16:1 ω6c; 25.2-25.4 %) and C19 :0 cyclo ω8c (11.9-15.4 %). The major respiratory quinone was Q-10. The genomic DNA G+C contents of PAMC 29128 and PAMC 29148T were 63.0 and 63.1 mol%, respectively. Their distinct phylogenetic position and some physiological characteristics support the proposal of Lichenihabitans gen. nov., with the type species Lichenihabitans psoromatis sp. nov. (type strain, PAMC 29148T=KCCM 43293T=JCM 33311T). Lichenihabitantaceae fam. nov. is also proposed.
Terrestrial N2O emissions and related functional genes under climate change: A global meta‐analysis

Nitrous oxide (N2O) emissions from soil contribute to global warming and are in turn substantially affected by climate change. However, climate change impacts on N2O production across terrestrial ecosystems remain poorly understood. Here, we synthesized 46 published studies of N2O fluxes and relevant soil functional genes (SFGs, that is, archaeal amoA, bacterial amoA, nosZ, narG, nirK and nirS) to assess their responses to increased temperature, increased or decreased precipitation amounts, and prolonged drought (no change in total precipitation but increase in precipitation intervals) in terrestrial ecosystem (i.e. grasslands, forests, shrublands, tundra and croplands). Across the data set, temperature increased N2O emissions by 33%. However, the effects were highly variable across biomes, with strongest temperature responses in shrublands, variable responses in forests and negative responses in tundra. The warming methods employed also influenced the effects of temperature on N2O emissions (most effectively induced by open‐top chambers). Whole‐day or whole‐year warming treatment significantly enhanced N2O emissions, but daytime, nighttime or short‐season warming did not have significant effects. Regardless of biome, treatment method and season, increased precipitation promoted N2O emission by an average of 55%, while decreased precipitation suppressed N2O emission by 31%, predominantly driven by changes in soil moisture. The effect size of precipitation changes on nirS and nosZ showed a U‐shape relationship with soil moisture; further insight into biotic mechanisms underlying N2O emission response to climate change remain limited by data availability, underlying a need for studies that report SFG. Our findings indicate that climate change substantially affects N2O emission and highlights the urgent need to incorporate this strong feedback into most climate models for convincing projection of future climate change.
Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses

The normal sequential development of the hip joint (HJ) was considered for the evaluation of the morphological and ultrastructural aspects of the joint cartilage of the proximal femoral head epiphysis in human fetuses between 16 to 31 week s f intra uterine life (IUL). Twenty human fetuses were fixed in 10 % formalin solution. Fetuses were divided into 4 groups (n=5): Group 1 ( 1): 16-19 weeks IUL; Group 2 (G2): 20-23 weeks IUL; Group 3 (G3): 24-27 weeks IUL and Group 4 (G4): 28-31 weeks IUL. The right moieties of the HJ were subjected to light microscopy to determine the chondrocyte area, volume, and density and the extracellu lar matrix (ECM) density. The collagen component in ECM was qualitatively evaluated using Safranin-O and picrosirius techniques und er polarized light. The left portions were analyzed using scanning electron microscopy (SEM). The advance of age revealed a gradua l increase in chondrocyte area and volume and in ECM density, and a decrease in chondrocyte density. The apparent prevalence of t ype II collagen fibers in G1 and type III collagen fibers in G4, as well as a balance between type I and III collagen fibers in G2 and G3 suggest a process of cartilaginous evolution and repair. The pantographic organization of the collagen fiber meshes from the depth to t he car ilage surface of the femoral head suggests that the arcade collagen network architecture starts at the fetal stage, regardless of the compressiv forces applied. The morphological data may contribute not only to a better understanding of the maturation and cartilage organi z tion in this area but also to serve as a theoretical basis for aspects related to diseases and joint malformations.
Involvement of D2 receptor in the NAc in chronic unpredictable stress-induced depression-like behaviors

Abstract D2 receptors (D2Rs) located in both pre- and postsynaptic membranes of medium spiny neurons (MSNs) in the nucleus accumbens (NAc) are involved in the stress response and associated behaviors. The role of D2Rs in chronic unpredictable stress (CUS)-induced depression-like behaviors is not clear. Quinpirole (a D2R agonist) and eticlopride (a D2R antagonist) were stereotactically delivered into the NAc before Sprague Dawley rats underwent CUS. CUS-induced depression-like behaviors were accompanied by a significant decrease in both the dopamine (DA) level and D2R expression in the NAc. Eticlopride reversed CUS-induced depression-like behavior and rescued the DA levels in the NAc, and microinjection of DA into the NAc of CUS individuals had the same effect as eticlopride. By contrast, delivery of quinpirole into the NAc of control animals induced depression-like behaviors accompanied by a decrease in the DA level in the NAc. These results show that DA plays a key role in CUS-induced depression-like behaviors and the D2R exerts a presynaptic negative feedback on DA levels during CUS. Microinjection of quinpirole into the NAc also decreased the level of the kalirin-7 protein in the NAc of both control and stressed animals, while eticlopride increased its level in the NAc of rats. In agreement with these results, intraperitoneal injection of eticlopride in mice also caused an increase in both the kalirin-7 protein level in the NAc and spine density in MSNs, while quinpirole reduced them. These results suggest that regulation of kalirin-7 through D2R in the NAc is a general pathway in rats and mice, and is involved in CUS-induced depression-like behaviors. Kalirin-7 may be directly regulated through the D2R postsynaptic pathway or indirectly through the presynaptic pathway in the NAc. The interaction between D2R and kalirin-7 needs to be investigated further.
Owners’ Attitudes toward Their Companion Dogs Are Associated with the Owners’ Depression Symptoms—An Exploratory Study in South Korea

Background: Various health benefits from the ownership of companion dogs have been studied from a One Health perspective. However, the preventive effects on depression are unclear, with inconsistent results across studies. We hypothesized that heterogeneity among owners would be related to the mixed results. Specifically, the difference in the strength of the bond between the owners and their companion dogs would modify the effect of dog ownership. As an exploratory study, we compared the depression symptoms of the owners with favorable attitudes toward their dogs, with those of the owners with unfavorable attitudes, to investigate the potential effect modification of owners’ attitudes on the association between the ownership and depression symptom. Methods: We conducted a web-based questionnaire survey of 654 19- to 39-year-old adults who had companion dogs in Seoul, South Korea, where a major health burden is depression among young adults. We measured the owners’ attitudes toward their dogs using the modified Pet Attitude Scale (PAS-M) and their depression symptoms using the short version of the Center for Epidemiologic Studies depression scale (CESD-10). Demographic and socioeconomic factors were measured to adjust for the association between attitude and depression symptoms. Multivariate logistic regression models were used in this study. Results: The owners who had less favorable attitudes toward their dogs (lower PAS-M scores) tended to have depression symptoms. The direction and significance were maintained either when the PAS-M variable was used as a continuous variable (odds ratio (OR) for one score increase in PAS-M was 0.95 (95% confidence interval (CI) = 0.94–0.96)) or as a categorical variable (OR for lower PAS-M was 3.19 (95% CI = 2.28 –4.47)). Conclusion: We found a positive significant association between owners’ depression symptoms and unfavorable attitudes toward their dogs, although causal direction could not be determined. Future studies should investigate the potential causal link.
Nonword Repetition and Word Learning in Children with Specific Language Impairment

Children with specific language impairment (SLI) are notorious for inconsistent use of grammatical morphemes, as well as a small vocabulary. This lack of vocabulary is linked to their difficulty in learning new words, which requires a strong phonological memory. Tasks of nonword repetition call upon this same skill. This overlap in skills suggests a strong relationship between the two tasks. The current study explores the relationship between nonword repetition performance and novel word learning abilities in preschool-aged children with SLI as compared to their typically developing (TD) agematched peers. Nine children with SLI and nine TD children completed a nonword repetition test (NRT) and a novel word learning task. Analysis of the relationship between the two tasks revealed few significant meaningful correlations for TD children and no significant correlations for those with SLI. The findings suggest that tasks of nonword repetition and encoding in word learning may not be tapping into the same mechanism, and that the relationship between the two is not as strong as first assumed.
QSAR‐Based Estimation of Species Sensitivity Distribution Parameters: An Exploratory Investigation

Ecological risk assessments are hampered by limited availability of ecotoxicity data. The present study aimed to explore the possibility of deriving species sensitivity distribution (SSD) parameters for nontested compounds, based on simple physicochemical characteristics, known SSDs for data‐rich compounds, and a quantitative structure–activity relationship (QSAR)‐type approach. The median toxicity of a data‐poor chemical for species assemblages significantly varies with values of the physicochemical descriptors, especially when based on high‐quality SSD data (from either acute median effect concentrations or chronic no‐observed‐effect concentrations). Beyond exploratory uses, we discuss how the precision of QSAR‐based SSDs can be improved to construct models that accurately predict the SSD parameters of data‐poor chemicals. The current models show that the concept of QSAR‐based SSDs supports screening‐level evaluations of the potential ecotoxicity of compounds for which data are lacking. Environ Toxicol Chem 2019;38:2764–2770. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC
Different Characteristics and Heritabilities of Alcohol Use Disorder Classes: A Population-Based Swedish Study.

AIMS The aims of the present study are to identify alcohol use disorder (AUD) classes among a population-based Swedish sample, determine if these classes differ by variables known to be associated with AUD and determine whether some AUD classes have stronger genetic influences than others. METHODS A latent class analysis (LCA), based on types of registrations, was conducted on Swedish individuals with an AUD registration born between 1960 and 1990 (N = 184,770). These classes were then validated using demographics; patterns of comorbidity with drug abuse, psychiatric disorders and criminal behavior; and neighborhood-level factors, i.e. peer AUD and neighborhood deprivation. The degree of genetic and environmental influence was also investigated. RESULTS The best-fit LCA had four classes: (a) outpatient/prescription, characterized by a mix of outpatient medical and prescription registrations, (b) low-frequency inpatient, characterized entirely by inpatient medical registrations, with the majority of individuals having one AUD registration, (c) high-frequency mixed, characterized by a mix of all four registration types, with the majority having four or more registrations and (d) crime, characterized almost entirely by criminal registrations. The highest heritability for both males and females was found for Class 3 (61% and 65%, respectively) and the lowest for Class 1 (20% for both), with shared environmental influences accounting for 10% or less of the variance in all Classes. CONCLUSIONS Using comprehensive, nationwide registry data, we showed evidence for four distinct, meaningful classes of AUD with varying degrees of heritability.
Radiotherapy and immunotherapy promote tumoral lipid oxidation and ferroptosis via synergistic repression of SLC7A11.

A challenge in oncology is to rationally and effectively integrate immunotherapy with traditional modalities including radiotherapy. Here, we demonstrate that radiotherapy induces tumor cell ferroptosis. Ferroptosis agonists augment and ferroptosis antagonists limit radiotherapy efficacy in tumor models. Immunotherapy sensitizes tumors to radiotherapy by promoting tumor cell ferroptosis. Mechanistically, IFNgamma derived from immunotherapy-activated CD8+ T cells and radiotherapy-activated ATM independently, yet synergistically repress SLC7A11, a unit of the glutamate-cystine antiporter xc-, resulting in reduced cystine uptake, enhanced tumor lipid oxidation and ferroptosis, and improved tumor control. Thus, ferroptosis is an unappreciated mechanism and focus for the development of effective combinatorial cancer therapy.