Miracle-dz/UniFAR-FTUs huggingface dataset:
Overexpression of mtlD gene in potato (solanum tuberosum L.), cv. Arinda improves salt tolerance

Potato as one of the world’s main tuber crops of the solanaceae family plays an important role in the economics scene. Since one of the major reasons for yield decreasing in the world are abiotic stresses. The aim of this research was producing transgenic potatoes with more tolerance to salinity stress and studying GMO potato features. To produce GMO plants, new gene (mtlD, mannitol -1phosphate dehydrogenase, E.C.1.1.1.17) was transferred to a plant by using agrobacterium plasmid. For the production of transformed potato plant resistant to salinity stress, mannitol, 1-phosphate dehydrogenase gene (mtlD, E.C.1.1.1.17) was used. At first mtld gene was isolated from pCabmtld plasmid of E. Coli and then was cloned in PBI121 plasmid. The promoters that have been most commonly used in the production of abiotic stress tolerant plants so far, include the CaMV 35S (Bhatnagar-mathur et al., 2007). This vector was inserted into Agrobacterium tumefaciens which was used for producing transformed potato plant and was stored in 28°c temperature. The freeze-thaw method (Holsters et al., 1978) was used for replacement of recombinant binary vector PBI-VHH into Agrobacterium tumefaciens strains LBA4404. Existence of recombinant gene in transgenic plants was confirmed by PCR technique. For final confirmation, the features of transgenic potato were studied. Briefly, the transgenic potatoes and nontransgenic potatoes lines showed the different amounts of tolerance to salinity stress because the transgenic lines produced mannitol which increased osmotic pressure in salinity stress. The salt tolerance of transgenic potato (+mtlD) was recorded higher than that of nontransgenic potato (-mtlD). Growth analyses include CGR (Crop Growth Rate) and RGR (Relative Growth Rate) in transgenic and nontransgenic potatoes lines were showed that +mtlD potatoes lines grew better than –mtlD potatoes lines in salinity stress. This approved that +mtlD had more tolerance to salinity stress due to production of mannitol. The comparison of linear regression equations of transgenic (+mtlD) and nontransgenic potatoes (-mtlD) showed the more tolerance of +mtlD to salinity stress than –mtlD potatoes. Osmotic pressure in this GMO plant was increased by producing of mannitol and consequently producing mannitol certify that the mtlD gene was successfully transferred to potato.
Support Vector Machine for Text Categorization using Principle Component Analysis in Data Mining

Data mining is the withdrawal of concealed prescient information also obscure data, examples, connections and learning by investigating the enormous informational collections which are hard to discover and distinguish with customary measurable techniques. The major issues in text categorization are classification accuracy and computation time. To overcome these issues, an efficient classification method is needed for high differentiation exactness as fine as minimizing the computation period. In this work, we propose the classification of data using support vector machine for text categorization along with principle component analysis. Bolster Vector Machines is a managed learning system with numerous attractive characteristics that make it a prevalent calculation. Principle Component Analysis (PCA) is the feature removal technique is used towards mine the features with in the text. Chi-Square is a further assortment technique it is used to selecting the features from removed features. Finally by this proposed work, the classification accuracy also computation period is improved than other existing algorithms in many applications
The Discontinuity of Independent Nursing Care Documentation Using the Fishbone Diagram: A Case Study

Quality nursing care documentation based on facts, accurate, complete, concise, organized, timely, easy to read, and continuity. Continuity of nursing care is fulfilled if the range of health services is appropriate, the availability of quality caregivers professionals and information related to patient care are delivered on an ongoing basis. The method used is a case study with observation, interviews, secondary data, and document review. The results showed that the documentation evaluation of nursing care for patients development in the midwifery and gynecology room in X Hospital in Jakarta had not reflected the continuity of nursing care. The causes include the lack of optimal competence in providing independent nursing care, not optimal nursing manager functions. The continuity is overcome by optimizing the nursing manager's role by making SOP integrated patient development note, revision of midwife's job description, review of guide to nursing practice. The electronic-based documentation system, placing midwives by the service area the authority and standard of the profession, the structured supervision schedule, and make sure the midwife's job description is by the policy.
Monocot Pseudanthia Revisited: Floral Structure of the Mycoheterotrophic Family Triuridaceae

The floral units of Triuridaceae, commonly regarded as flowers, may equally plausibly be interpreted as pseudanthia, i.e., modified and highly reduced partial inflorescences. This hypothesis is new for Triuridaceae, but earlier authors have noted strong similarities between the inflorescences of Cyclanthaceae and Pandanaceae (Pandanales) and the putatively pseudanthial floral units of some Alismatales (e.g., Potamogeton). The morphology of the floral units of Triuridaceae, particularly the female and hermaphrodite structures, closely resembles a smaller version of a Pandanus pistillate inflorescence. In some species of Pandanus, the female flower consists of a single uniovulate carpel with a single stigma, as in Triuridaceae. This striking morphological similarity is remarkable in the context of recent evidence from a single 18S nuclear rDNA sequence for Sciaphila that placed it as sister to Pandanaceae within Pandanales. However, because pseudanthia have been reported in both Alismatales and Pandanales, a pseudanthial interpretation does not in itself support or refute a close relationship with either of these orders, and a more detailed analysis of the phylogenetic placement of Triuridaceae is desirable. A pseudanthial interpretation provides a possible explanation for the inside‐out “flowers” of Lacandonia schismatica, in which the three stamens are distal to the numerous carpels; these may not be flowers but highly reduced partial inflorescences. Pseudanthia have apparently evolved several times in monocots and other basal angiosperms, and it may be useful to assess both inflorescences and flowers more flexibly in these groups in which developmental pathways are less canalized than in more derived angiosperm lineages.
INTERTIDAL ASSEMBLAGES ON COASTAL DEFENCE STRUCTURES IN SINGAPORE II. CONTRASTS BETWEEN ISLANDS AND THE MAINLAND

– In Singapore, breakwaters have replaced natural rocky shores as the predominant hardsubstratum intertidal coastal habitat on both the main island as well as the smaller islands to the south. These form isolated and non-contiguous areas of relatively homogenous rock (granite), separated by sandy shore or water. Insular assemblages of species at island locations are expected to be more stochastic in organisation than those on the mainland, due in part to variable patterns of colonisation and extinction. As such, there should be differences between the mainland and islands in terms of assemblage composition, structure and in the variability of abundances of taxa at a range of spatial scales. This study examines the structure of assemblages on seawall habitats located on the Singapore mainland and its southern islands. Analyses revealed differences between island and the mainland in the structure, but not the composition of intertidal assemblages, and also in the magnitude of variation in abundances of individual taxa at the spatial scales examined. Differences were more pronounced in assemblages lower on the shore than in high shore and supralittoral assemblages. In addition, assemblages tended to be more similar within locations, but there was no tendency for variability in the mean abundances of any taxa to be consistently higher at any habitat or spatial scale. On the whole, the results do not support the model of higher stochasticity of assemblages on artificial intertidal rocky habitats on islands. Instead, it revealed that high spatial variability at mainland locations could be linked to highly localised environmental conditions. Community organisation on these seemingly homogenous structures is shown to be complex and idiosyncratic. Factors and mechanisms influencing assemblage structure in natural rocky intertidal habitats are likely to operate differently on these artificial habitats, and should be examined critically prior to management decisions assuming or relying on the surrogacy of these structures as alternative marine habitats.
Do multiple-trial games better reflect prosocial behavior than single-trial games?

Most prior research on the external validity of mixed-motive games has studied only one single game version and/or one specific type of real-life prosocial behavior. The present study employs a different approach. We used multiple game trials — with different payoff structures — to measure participants’ behavior in the Prisoner’s Dilemma, the Commons Dilemma, and the Public Goods Dilemma. We then examined the associations between these aggregated game behaviors and a wide set of self-reported prosocial behaviors such as donations, commuting, and environmental behaviors. We also related these prosocial behavior measures to a dispositional measure of prosociality, social value orientation. We report evidence that the weak statistical relationships routinely observed in prior studies are at least partially a consequence of failures to aggregate. More specifically, our results show that aggregation over multiple game trials was especially effective for the Prisoner’s Dilemma, whereas it was somewhat effective for the Public Goods Dilemma. Yet, aggregation on the side of the prosocial behaviors was effective for both these games, as well as for social value orientation. The Commons Dilemma, however, turned out to yield invariably poor relationships with prosocial behavior, regardless of the level of aggregation. Based on these findings, we conclude that the use of multiple instances of game behavior and prosocial behavior is preferable to the use of only a single measurement.
The Chromosome Numbers of North American Coreopsis with Phyletic Interpretations

A summary of the known chromosome numbers for the North American species of Coreopsis (Compositae) is presented. New chromosome counts are presented for C. delphinifolia (2n = 52), C. major var. major (n = 13), C. major var. stellata (n = 13), and C. verticillata (n = 26). Including this paper, chromosome counts of 43 species of the approximately 50 species described for North America have been reported; counts from all sections are available. The major phyletic lines in the genus are suggested, based on correlation between 17 morphological trends and presumed chromosomal derivation. The following new combinations are suggested: C. wrightii (A. Gray) H. M. Parker (based on C. basalis var. wrightii), C. tinctoria Nutt. var. similis (F. Boynton) H. M. Parker (based on C. similis), and C. tinctoria Nutt. var. atkinsoniana (Dougl.) H. M. Parker (based on C. atkinsoniana). A pattern of descending aneuploidy is indicated for the North American species of Coreopsis, with a primitive base number of x = 14. The bearing of this base number on that for the tribe Heliantheae is considered.
Performance of Bali Heifers and Calves Prior to Weaning in a Feedlot System

A study on performance of Bali heifers and their first calves kept in a feedlot system was carried out using 20 heifers fed two feeding regimes namely elephant grass only ( group A ) and a mixture of 40 % elephant grass and 60 % concentrate containing 20.7 % CP and 77.3 % TDN ( group B ). Liveweight up to calving, production and quality of milk, calfbirth, and daily gain prior to weaning in this cattle breed were significantly affected (P>0.05) by concentrate supplementation. However, service per conception and gestation length was not significantly (P>0.05) different between the two groups of feeding regimes. It seemed that in the short term, improvement in management particularly feeding a higher quality ration was needed to overcome the problem in performance degradation of Bali cattle which has become some concerned recently.
Long-Term Outcomes of Behçet's Syndrome-Related Uveitis: A Monocentric Italian Experience

Objectives To examine demographic and clinical characteristics and long-term visual outcome in a cohort of Italian patients affected by Behçet's uveitis (BU). Materials and Methods Retrospective chart review of 47 patients with BU attending our unit between January 2018 and December 2019. Ophthalmologic manifestations, best-corrected visual acuity (BCVA), fluoroangiography and optical coherence tomography findings, and ocular complications were recorded. Predictive factors of a poor visual outcome and long-term complications were also investigated. Results Forty-seven patients (23 males and 24 females) for a total of 84 eyes were enrolled. Uveitis was bilateral in 37 (78.7%) patients with panuveitis being the most frequent anatomical pattern (40 out of 84 eyes), whereas 27 eyes presented a posterior uveitis. Isolated anterior uveitis was detected in 16 eyes. A significant improvement of median BCVA between baseline and last follow-up values was detected (p = 0.042). A higher risk of poor visual prognosis was observed in patients with uveitis duration greater than 15 years (p = 0.019). A significant resolution of retinal vasculitis was detected between baseline and last follow-up evaluation (p < 0.0001) whereas the mean ± SD macular thickness did not decrease significantly between baseline (376.00 ± 97.45 μm) and last follow-up evaluation (338.08 ± 55.81 μm). Forty-two eyes developed 57 complications during the disease course. Cataract was the most frequent (n = 12), followed by epiretinal membranes (n = 11) and cystoid macular edema (n = 6). The following variables were identified as predictors of long-term complications: human leukocyte antigen- (HLA-) B51 (p = 0.006), panuveitis (p = 0.037), and a uveitis duration of more than 15 years (p = 0.049). Conclusions In Italian patients, BU typically arises in the third decade and predominantly manifests as a bilateral posterior uveitis or panuveitis. Its duration is associated with a poor visual prognosis. Uveitis duration, the presence of HLA-B51, and panuveitis are predictors of long-term structural complications, thus representing main drivers in the treatment decision-making.
Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model

The adipose-derived stromal vascular fraction (SVF) is an effective source for autologous cell transplantation. However, the quality and quantity of SVFs vary depending on the patient's age, complications, and other factors. In this study, we developed a method to reproducibly increase the cell number and improve the quality of adipose-derived SVFs by surgical procedures, which we term “wound repair priming. ” Subcutaneous fat from the inguinal region of BALB/c mice was surgically processed (primed) by mincing adipose parenchyma (injury) and ligating the subcutaneous fat-feeding artery (ischemia). SVFs were isolated on day 0, 1, 3, 5, or 7 after the priming procedures. Gene expression levels of the primed SVFs were measured via microarray and pathway analyses which were performed for differentially expressed genes. Changes in cellular compositions of primed SVFs were analyzed by flow cytometry. SVFs were transplanted into syngeneic ischemic hindlimbs to measure their angiogenic and regeneration potential. Hindlimb blood flow was measured using a laser Doppler blood perfusion imager, and capillary density was quantified by CD31 staining of ischemic tissues. Stabilization of HIF-1 alpha and VEGF-A synthesis in the SVFs were measured by fluorescent immunostaining and Western blotting, respectively. As a result, the number of SVFs per fat weight was increased significantly on day 7 after priming. Among the differentially expressed genes were innate immunity-related signals on both days 1 and 3 after priming. In primed SVFs, the CD45-positive blood mononuclear cell fraction decreased, and the CD31-CD45-double negative mesenchymal cell fraction increased on day 7. The F4/80-positive macrophage fraction was increased on days 1 and 7 after priming. There was a serial decrease in the mesenchymal-gated CD34-positive adipose progenitor fraction and mesenchymal-gated CD140A-positive/CD9-positive preadipocyte fraction on days 1 and 3. Transplantation of primed SVFs resulted in increased capillary density and augmented blood flow, improving regeneration of the ischemic limbs. HIF-1 alpha was stabilized in the primed cutaneous fat in situ, and VEGF-A synthesis of the primed SVFs was on a peak on 5 days after priming. Wound repair priming thus resulted in SVFs with increased number and augmented angiogenic potential.
Lactobacillus plantarum And Inulin: Therapeutic Agents to Enhance Cardiac Ob Receptor Expression and Suppress Cardiac Apoptosis in Type 2 Diabetic Rats

Background T2DM may cause increased levels of oxidative stress and cardiac apoptosis through elevated blood glucose. The present study investigated the effects of Lactobacillus plantarum (L. plantarum) as a probiotic strain and inulin as a prebiotic supplement on cardiac oxidative stress and apoptotic markers in type 2 diabetes mellitus (T2DM) rats. Methods A high-fat diet and a low dose of streptozotocin were used to induce type 2 diabetes. The rats were divided into six groups which were supplemented with L. plantarum, inulin, or their combination for 8 weeks. Results The results showed improved activity of cardiac antioxidant parameters including total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (P < 0.001, P < 0.01, and P < 0.01, respectively) and decreased level of cardiac malondialdehyde (MDA) concentration (P < 0.05). These changes were accompanied with increased protein expression of cardiac obesity receptor (Ob-R) (P = 0.05) and reduced apoptotic markers such as tumor necrosis factor-alpha (TNF-α), Fas ligand (FasL), and caspase proteins (P < 0.001, P = 0.003, and P < 0.01, respectively) in T2DM rats after concurrent L. plantarum and inulin supplementation. Moreover, a remarkable correlation of cardiac Ob-R and oxidative stress parameters with cardiac apoptotic markers was observed (P < 0.01). Conclusion The concurrent use of L. plantarum and inulin seems to be beneficial, as they can lead to decreased heart complications of T2DM via reducing cardiac apoptotic markers.
Yolkin Isolated from Hen Egg Yolk as a Natural Immunoregulator, Activating Innate Immune Response in BMDM Macrophages

One of the goals of biomedical sciences is to search and identify natural compounds that are safe, have no side effects, and possess immunostimulatory activity. It has been proven that medicines of natural origin can be effective agents, supporting the therapy of many diseases, not only in the weakened immune system of the body but also in the prevention of many diseases in healthy people. It has been shown that yolkin, a polypeptide complex isolated from hen egg yolk as a fraction accompanying immunoglobulin Y (IgY), possesses potential biological activity. However, the mechanism of its action has not been explained. The objective of this investigation was to examine the molecular mechanisms of innate immune response, activated in response to yolkin, in murine bone marrow-derived macrophages (BMDM). It was shown that yolkin induced phosphorylation of extracellular signal-kinases (ERK1/2) and c-Jun N-terminal kinase (JNK) and upregulated expression and production of type I interferons, TNF-α (tumor necrosis factor α), and nitric oxide (NO), in BMDM cells. Using pharmacological inhibitors of ERK 1/2 and JNK kinases, we revealed that the JNK signaling cascade is required for yolkin-induced inducible NOS expression and upregulation of NO production in mouse macrophages. Using the TLR4-deficient BMDM cell line, we established that yolkin can activate macrophages in a TLR4-dependent manner. It was also shown that NO, TNF-α, and type I IFNs (α/β) produced by BMDM cells in response to yolkin triggered antiviral activity. These data indicate that yolkin affects the regulation of the immune system and antiviral response; therefore, it can be used as an effective immunostimulator of the innate immunity or as a supplement of the conventional therapy of immunodeficiency.
Systems Pharmacology-Dissection of the Molecular Mechanisms of Dragon's Blood in Improving Ischemic Stroke Prognosis

Materials and Methods (1) Based on system-pharmacology platform, the potential active compounds of DB are screened out according to ADME. (2) The ischemic stroke-related targets are predicted by utilizing these active compounds as probes, mapping the targets to the CTD database to establish a molecular-target-disease network. (3) To analyze the mechanism of DB treatment for the prognosis of ischemic stroke, we used the Metascape and DAVID databases to construct “ischemic stroke pathways”. (4) PC12 cells were used to explore the protective effect of loureirin B on oxygen-glucose deprivation/reperfusion (OGD/R) injury, and BV-2 cells were used to determine the anti-inflammation effect of 4′,7-dihydroxyflavone. Results Finally, we obtained 38 active compounds and 58 stroke-related targets. Network and pathway analysis indicate that DB is effective in the treatment of ischemic stroke by enhancing cell survival and inhibiting inflammatory and antiplatelet activation. In in vitro experiments, the main component loureirin B promoted the expression of HO-1 and Bcl-2 via positive regulation of PI3K/AKT/CREB and Nrf2 signaling pathways in PC12 cells against OGD/R damage. And the anti-inﬂammatory activity of 4′,7-dihydroxyflavone was related to the inhibition of COX-2, TNF-α, and IL-6 in LPS-induced BV-2 cells. Conclusions In our study, the results illustrated that DB in improving ischemic stroke prognosis may involve enhancing cell survival and antioxidant, anti-inflammation, and antiplatelet activities.
Limitations of Systemic Oncological Therapy in Breast Cancer Patients with Chronic Kidney Disease

Breast cancer is the most common malignancy, affecting middle-age and older women frequently suffering from other chronic diseases, including chronic kidney disease. The risk of breast cancer development in women on renal replacement therapy (peritoneal dialysis and haemodialysis) is higher than in the general population. Chronic kidney disease does not limit surgical treatment or radiotherapy; however, it affects the pharmacokinetics of drugs used in the systematic treatment to a different extent, increasing their toxicity and the risk of adverse drug reactions. This article summarizes the current knowledge (published studies accessed through PUBMED) on drugs used in chemotherapy, hormone therapy, anti-HER2 drugs, CDK4/6 inhibitors, PARP inhibitors, and immune therapy in breast cancer patients undergoing dialysis. We discuss the data, the optimal choice of the chemotherapeutic protocol, and the administration of drugs in a specific time relation to the haemodialysis session to ensure the most effective and safe treatment to breast cancer patients.
Longevity and Aging in Vertebrate Evolution

Mammalian lifespan has a close allometric association with brain weight, body weight, metabolic rate, and body temperature. Longevity, then, if governed by natural selection acting on positive genetic mechanisms for stabilizing life processes, rather than by the random accumulation of ''senescence genes. '' This paper compares the social implications of these hypotheses.