Miracle-dz/UniFAR-FTUs huggingface dataset:
Context-dependent signalling of CXC chemokine receptor 4 (CXCR4) and atypical chemokine receptor 3 (ACKR3)

Resonance Energy Transfer; Receptor; cluster of differentiation; Abstract G protein-coupled receptors (GPCRs) are regulated by complex molecular mechanisms, both in physiological and pathological conditions, and their signalling can be intricate. Many factors influence their signalling behaviour, including the type of ligand that activates the GPCR, the presence of interacting partners, the kinetics involved or their location. The two CXC type chemokine receptors CXCR4 and ACKR3, both members of the GPCR superfamily, are important and established therapeutic targets in relation to cancer, HIV infection and inflammatory diseases. Therefore, it is crucial to understand how the signalling of these receptors works to be able to specifically target them. In this review, we discuss how the signalling pathways activated by CXCR4 and ACKR3 can vary in different situations. G protein signalling of CXCR4 depends on the cellular context and discrepancies exist depending on the cell lines used. ACKR3, as an atypical chemokine receptor, is generally reported to not activate G proteins, but can broaden its signalling spectrum upon heteromerisation with other receptors, such as CXCR4, endothelial growth factor receptor (EGFR) or the α 1 -adrenergic receptor ( α 1 -AR). Also, CXCR4 forms heteromers with CCR2, CCR5, the Na + /H + exchanger regulatory factor 1 (NHERF1), CXCR3, α 1 -AR and the opioid receptors, which results in differential signalling to that of the monomeric subunits. In addition, CXCR4 is present on membrane rafts, but can go into the nucleus during cancer progression, probably acquiring different signalling properties. In this review, we also provide an overview of the currently known critical amino acids involved in CXCR4 and ACKR3 signalling. studies show that the arrestins attenuate G protein signalling. In human embryonic cells, overexpression of CXCR4 with either β -arrestins reduced inhibition of cAMP potency of G α i upon CXCR4 activation with CXCL12 decreased in cells co-expressing ACKR3. Moreover, ACKR3 co-expression with CXCR4 in HEK293 cells induced a conformational change between the pre-coupled CXCR4-YFP and G α i -Rluc. The same study also demonstrated that knockdown of ACKR3 expression in T lymphocytes resulted in more potent migration at lower CXCL12 concentrations, addressing the scavenging function of ACKR3 (Levoye et al., 2009).
RMHP_A_287382 743..756

1University of Claude Bernard Lyon 1, HESPER Laboratory, Lyon, EA 7425, France; 2CRIFONDO, Caribbean Renaissance Initiative, Département de Gérontologie, Martinique, France; 3Hospices Civils De Lyon, Pôle De Santé Publique, Public Health, Lyon, France Rationale: Although the literature recognizes the importance of older individuals’ subjective perceptions about their living environments, past studies on the subject have been primarily restricted to nursing home settings. Objective: This study aimed to better understand the experiences, perceptions, coping mechanisms, and needs of older people living in Martinique who had to modify their living environment because of a decline of autonomy. Design: Qualitative study using content analysis. Methods: Semi-structured one-on-one interviews were conducted with older people living in three different types of environment i) at home with professional support, ii) in a foster care family, iii) in a nursing home. Interviews were conducted until data saturation was reached. A conventional content analysis approach was used. Results: Thirty-four participants were interviewed. Subjects perceived ageing as a factor leading to changes in their living environment. However, they did not spontaneously evoke their functional/ structural impairments nor their activity limitations as if the change of living environment could reduce the perceived loss of autonomy by maintaining an acceptable participation. Participants mostly experienced change as both inevitable and as a relief as it took them out of isolation and domestic hardship. This reaction was somewhat facilitated by spiritual beliefs as the changes were interpreted as the will of spiritual forces. Family and social relationships appeared to be more important determinants of participants’ perception of changes in living environment than was health status. We found differences between the three groups regarding familial relationship, fear of death, acceptance of change, and unmet needs. Unmet needs were particularly expressed by those living in foster care. The concept of “feeling at home”, which emerged as essential for all participants, was lacking in foster care families. Conclusion: Perceived autonomy, including not only functional/structural impairments, but also, social interactions, should be carefully considered when developing support services for older individuals.
Comparison of Anterior Segment Parameters and Axial Length Using Two Scheimpflug Devices with Integrated Optical Biometers

Purpose: To assess the repeatability of anterior segment parameters and axial length (AL) using Pentacam AXL and Galilei G6 and the agreement between both devices. Materials and Methods: Eighty-four eyes of 84 participants were measured prospectively with two devices. Outcome measurements included corneal curvatures, anterior chamber depth (ACD), AL, pupil size, and white-to-white distance (WTW). Intra-device repeatability was assessed using intraclass correlation coefficient (ICC), within-subject standard deviation (Sw), test–re-test repeatability (TRT=2.77 Sw), and coefficient of variation (CoV). Agreement between two devices was analyzed using Bland–Altman plots. Results: For each device, the Sw of corneal curvatures, ACD, and AL were lower than 0.25 D, 0.04 mm, and 0.04 mm, respectively. The ICC was higher than 0.90 in all parameters measured by Pentacam AXL, whereas three parameters measured by Galilei G6 (steep meridian at anterior and posterior cornea, and pupil size) were lower than 0.90. Comparing to Galilei G6, Pentacam AXL led to significantly lower mean anterior cornea curvatures (Km) with the mean difference (95% level of agreement; LoA) of −0.12 D (−0.36, 0.12, P <0.001). For ACD, there was no significant difference between the two devices. Pentacam AXL led to significantly lower AL, pupil size, and WTW, with the mean differences (95% LoA) of −0.04 mm (−0.35, 0.27), −0.18 mm (−0.71, 0.35), and −0.35 mm (−0.61, −0.10), respectively. Conclusion: We found good repeatability of corneal curvature, ACD, and AL in both devices. Most parameters obtained from Pentacam AXL were statistically significantly different from those obtained from Galilei G6, except for steep meridians and ACD.
A novel rapid assay for evaluating the efficacy of biocides to inhibit the development of marine photoautotrophic biofilms

In this paper a novel settlement and growth assay is presented that uses field-collected marine 6 photoautotrophic biofilms as inoculum. The multitude of indigenous species that are the 7 potential foulers are therefore included in the assay, which overcomes the limitation of testing 8 only those species that can be cultivated in the laboratory. The assay was evaluated using 9 eight antifouling biocides. The methodological considerations are discussed thoroughly and 10 full concentration response curves are presented for all tested biocides. The efficacy ranking 11 based on EC 98 values from the most to the least efficacious compound is as follows: copper 12 pyrithione > TPBP > DCOIT > tolylfluanid > zinc pyrithione > medetomidine > copper 13 (Cu 2+ ). The algaecide irgarol did not cause a full inhibition of settlement and growth but the 14 inhibition leveled out at 95% already at 30 nmol l -1 , at a concentration that is clearly lower 15 than for any other of the tested biocides. 16 17 18
A Proposed Software Structural Design for Instigating Power Management Practices within Intelligent TAGS

— Power consumption has become a serious issue in the development of remote mobile devices such as intelligent tags where charging and recharging of the battery cannot be done frequently. Longevity of the battery has to be increased by power saving. Many existing techniques address the issue of power management in embedded systems. This paper has implemented an efficient method for power management in TAG. In this paper software architecture has been presented for implementing the power management method. Embedded code has been developed and the same is experimented by building an embedded system for the purpose.
Synapse Device Based on Charge-Trap Flash memory for Neuromorphic Application

—This paper proposes a synaptic device based on charge trap flash (CTF) memory that has good CMOS compatibility and excellent reliability compared to other synaptic devices. Using hot-electron injection (HEI) and hot-hole injection (HHI), we designed the operation method to fulfill incremental-step-pulse program (ISPP). To demonstrate the ability of the device for neuromorphic, the device simulation (TCAD) and the MATLAB simulation were performed simultaneously. We also implemented the multi-level operation.
Numerische Methoden für hochdimensionale parabolische Gleichungen am Beispiel von Optionspreisaufgaben

Zunehmend an Bedeutung in einer Vielzahl von Anwendungsfeldern gewinnen hochdimensionale Aufgabenstellungen, oft in der Form von partiellen Differentialgleichungen. Kernpunkt dieser Arbeit sind Diskretisierungsverfahren speziell fur diese Problemklasse, wobei en passent auch auf die untrennbar verbundenen Aspekte iterativer Gleichungslosung und speziell auch der Modellreduktion (hier in Form von asymptotischer Analysis zur Reduzierung der Dimension) eingegangen wird. Als Anwendungsbeispiel werden Optionspreisaufgaben in bis zu dreisig Dimensionen studiert, da viele Produkte am Markt durch die Anzahl der Faktoren direkt auf hochdimensionale (Un-)Gleichungen fuhren und auserdem die oft unabdingbare stochastische Modellierung von Marktdaten die Dimension weiter hochtreibt. Die Basis dazu bilden dunne Gitter. Mittels der Kombinationstechnik wird die Dunngitter-Losung aus Finite-Differenzen-Losungen auf einer Familie anisotroper kartesischer Gitter extrapoliert, wodurch die Zahl der Freiheitsgrade entscheidend reduziert und der Algorithmus auf naturliche Weise parallelisiert werden kann. Aus einer geeigneten Fehlerdarstellung am kartesischen Gitter werden (Dunngitter-)Fehlerabschatzungen in einer geschlossenen Form fur beliebige Dimensionen abgeleitet und ein zusatzlicher multivariater Extrapolationsschritt fur eine hohere Konvergenzordnung motiviert. Dies erlaubt die numerische Differentiation mit hinreichender Genauigkeit. Optimale Komplexitat des Gesamtalgorithmus sowie eine effiziente Lastverteilung werden durch robuste Mehrgitterverfahren mit Block-Glattern und angepassten Transferoperatoren an freien Randern (bei Amerikanischen Optionen) erzielt. Am Beispiel von Basket-Optionen wird schlieslich demonstriert, wie Probleme, deren Dimensionalitat den mit dunnen Gittern behandelbaren Rahmen (ungefahr sechs) ubersteigt, mittels Hauptkomponentenanalyse und asymptotischer Analysis durch Aufgaben drastisch reduzierter Dimension mit hoher Genauigkeit approximiert werden konnen.
(2015). Alternative splicing of TIA-1 in human colon cancer regulates VEGF isoform expression, angiogenesis, tumour growth and bevacizumab resistance. Molecular (1), 167-78.

The angiogenic capability of colorectal carcinomas (CRC), and their susceptibility to antiangiogenic therapy, is determined by expression of vascular endothelial growth factor (VEGF) isoforms. The intracellular protein T-cell Intracellular Antigen (TIA-1) alters post-transcriptional RNA processing and binds VEGF-A mRNA. We therefore tested the hypothesis that TIA-1 could regulate VEGF-A isoform expression in colorectal cancers. TIA-1 and VEGF-A isoform expression was measured in colorectal cancers and cell lines. We discov-ered that an endogenous splice variant of TIA-1 encoding a truncated protein, short TIA-1 (sTIA-1) was expressed in CRC tissues and invasive K-Ras mutant colon cancer cells and tissues but not in adenoma cell lines. sTIA-1 was more highly expressed in CRC than in normal tissues and increased with tumour stage. Knockdown of sTIA-1 or over-expression of full length TIA-1 (ﬂTIA-1) induced expression of the anti-angiogenic VEGF isoform VEGF-A 165 b. Whereas ﬂTIA-1 selectively bound VEGF-A 165 mRNA and increased translation of VEGF-A 165 b, sTIA-1 prevented this binding. In nude mice, xenografted colon cancer cells over-expressing ﬂTIA-1 formed smaller, less vascular tumours than those expressing sTIA-1, but ﬂTIA-1 expression inhibited the effect of anti-VEGF antibodies. These results indicate that alternative splicing of an RNA binding protein can regulate isoform
An analysis for North American firms

This study examines the relationship between the CEO and CFO gender in large, public North American firms and credit risk, through the analysis of filings and delisting for bankruptcy, from 2007 to 2016. Using panel logit regressions for two models with similar specifications to those used by Campbell et al. (2008), I find with some statistical significance that, everything else constant, firms with females in these top positions are only less than 0.02 percentage points more likely to file or be delisted for bankruptcy, thus concluding that gender has relatively no effect on firm creditworthiness.
Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells - liquid biopsies for monitoring pregnancy

Our understanding of how cells communicate has undergone a paradigm shift since the recent recognition of the role of exosomes in intercellular signaling. In this study, we investigated whether oxygen tension alters the exosome release and miRNA profile from extravillous trophoblast (EVT) cells, modifying their bioactivity on endothelial cells (EC). Furthermore, we have established the exosomal miRNA profile at early gestation in women who develop pre-eclampsia (PE) and spontaneous preterm birth (SPTB). HTR-8/SVneo cells were used as an EVT model. The effect of oxygen tension (i.e. 8% and 1% oxygen) on exosome release was
Systems Biology: Confronting the Complexity of Cancer Cancer Research

The AACR-NCI Conference "Systems Biology: Confronting the Complexity of Cancer" took place from February 27 to March 2, 2011, in San Diego, CA. Several themes resonated during the meeting, notably (i) the need for better methods to distill insights from large-scale networks, (ii) the importance of integrating multiple data types in constructing more realistic models, (iii) challenges in translating insights about tumorigenic mechanisms into therapeutic interventions, and (iv) the role of the tumor microenvironment, at the physical, cellular, and molecular levels. The meeting highlighted concrete applications of systems biology to cancer, and the value of collaboration between interdisciplinary researchers in attacking formidable problems. Cancer Res; 71(18); 1 – 4. (cid:1) 2011 AACR. a staggered treatment by EGFR inhibition by rewires signaling in BT20 cell lines, to DNA damage. A partial least squares model based on a 35-protein phosphoprotein assay that EGFR inhibition reactivates a Caspase-8 dependent pathway required for
Lumbar Spine Reduce the Risk of Adjacent Segment Disease? Do PEEK Rods for Posterior Instrumented Fusion in the

Background: Polyetheretherketone (PEEK) rods were clinically introduced in the mid-2000s as an alternative to titanium (Ti) rods for posterior instrumented lumbar spine fusion, theorized to reduce the risk of adjacent segment disease (ASD). However, few studies have follow-up beyond 2 years. Consequently, we conducted a matched cohort study using data from Kaiser Permanente’s spine registry to compare the 2 rod systems and risk for outcomes. Methods: Patients aged (cid:2) 18 undergoing first posterior lumbar fusion for a degenerative diagnosis from 2009 to 2018 using either a PEEK or a Ti rod were identified. Fusions using Ti rods were 2:1 propensity score matched to PEEK rods on the following factors: patient age, body mass index, smoking, American Society of Anesthesiologists classification, diagnosis, interbody use, bone morphogenic protein use, number of levels fused, fusion levels, and operative year. The matched sample included 154 PEEK and 308 Ti fusions. We used Cox regression to evaluate ASD and nonunion, and logistic regression to evaluate 90-day emergency department (ED) visit, readmission, and complication. Results: We did not observe a difference in risk for ASD (hazard ratio ¼ 1.02, 95% confidence interval [CI] ¼ 0.66– 1.59) or ED visit (odds ratio [OR] ¼ 0.88, 95% CI ¼ 0.48–1.59). A lower likelihood of readmission (OR ¼ 0.34, 95% CI ¼ 0.13–0.94) was observed following PEEK fusion compared with Ti. No nonunions or 90-day complications were observed for the PEEK group; 5 (2-year cumulative incidence ¼ 0.7%) nonunions and 4 (1.3%) complications were observed for the Ti group. Conclusions: Our multicenter study did not support the hypothesis that PEEK rods are associated with a lower ASD risk. Reasons for readmission need to be identified to better understand the differences observed here. Further study of patients with TLIF using Ti and PEEK rods and posterolateral fusion with Ti and PEEK rods is needed. Clinical Relevance: The present study adds to the literature supporting their midterm effectiveness of PEEK rods compared with Ti rods for both their safety and their effectiveness at the 5–7-year follow-up.
Inﬂammatory cytokines (IL-1 (cid:97) , TNF- (cid:97) ) and LPS modulate the Ca 2 (cid:49) signaling pathway in osteoblasts

Inﬂammatory cytokines (IL-1 (cid:97) , TNF- (cid:97) ) and LPS modulate the Ca 2 (cid:49) signaling pathway in osteoblasts. Am. J. Physiol. 274 ( Cell Physiol. 43): C1686–C1698, 1998. —Locally derived growth factors and cytokines in bone play a crucial role in the regulation of bone remodeling, i.e., bone formation and bone resorption processes. We studied the effect of interleukin (IL)-1 (cid:97) , tumor necrosis factor (TNF)- (cid:97) , and Escherichia coli lipopolysaccharide (LPS) on the hormone-activated Ca 2 (cid:49) message system in the osteoblastic cell line UMR-106 and in osteoblastic cultures derived from neonatal rat calvariae. In both cell preparations, IL-1 (cid:97) , TNF- (cid:97) , and LPS did not alter basal intracellular Ca 2 (cid:49) concentration ([Ca 2 (cid:49) ] i ) but attenuated Ca 2 (cid:49) transients evoked by parathyroid hormone (PTH) and PGE 2 in a dose (1–100 ng/ml)- and time (8–24 h)- dependent fashion. The cytokines modulated hormonally induced Ca 2 (cid:49) inﬂux (estimated by using Mn 2 (cid:49) as a surrogate for Ca 2 (cid:49) ) as well as Ca 2 (cid:49) mobilization from intracellular stores. The latter was linked to suppressed production of hormonally induced inositol 1,4,5-trisphosphate. The effect of cytokines on [Ca 2 (cid:49) ] i was abolished by the tyrosine kinase inhibitor herbimycin A (50 ng/ml). The cytokine’s effect was, however, independent of nitric oxide (NO) production, since NO donors (sodium nitroprusside) as well as permeable cGMP analogs augment, rather than attenuate, hormonally induced Ca 2 (cid:49) transients in osteoblasts. Given the stimulatory role of cytokines on NO production in osteoblasts, the dispar- ate effects of cytokines and NO on the Ca 2 (cid:49) signaling pathway may serve an autocrine/paracrine mechanism for modulating the effect of calciotropic hormones on bone metabolism. Antigen-antibody precipitation was done by 100% ethanol. Results are expressed as picomoles of cAMP per 10 6 cells.
for the detection of reveals their presence in a range of environmental samples.

A bioassay Cobamides are a family of enzyme cofactors that include vitamin B 12 (cobalamin) and are produced solely by prokaryotes. Structural variability in the lower axial ligand has been observed in cobamides produced by diverse organisms. Of the three classes of lower ligands, the benzimidazoles are uniquely found in cobamides, whereas the purine and phenolic bases have additional biological functions. Many organisms acquire cobamides by salvaging and remodeling cobamides or their precursors from the environment. These processes require free benzimidazoles for incorporation as lower ligands, though the presence of benzimidazoles in the environment has not been previously investigated. Here, we report a new puriﬁcation method and bioassay to measure the total free benzimidazole content of samples from microbial communities and laboratory media components. The bioassay relies on the “calcoﬂuor-bright” phenotype of a bluB mutant of the model cobalamin-producing bacterium Sinorhizobium meliloti . The concentrations of individual benzimidazoles in these samples were measured by liquid chromatography-tandem mass spectrometry. Several benzimidazoles were detected in subpicomolar to subnanomolar concentrations in host-associated and environmental samples. In addition, benzimidazoles were found to be common contaminants of laboratory media components. These results suggest that benzimidazoles present in the environment and in laboratory media have the potential to inﬂuence microbial metabolic activities.
Effect of bendiocarb based (Ficam® 80% WP) Indoor Residual Spraying on feeding patterns of malaria vectors in Burkina Faso, West Africa. CURRENT STATUS: POSTED

Background : Vector control is a key component in malaria prevention strategies in many malaria endemic countries in Africa. In Burkina Faso, malaria transmission is seasonal in most parts of country, so a single round of spraying should provide effective protection against malaria, provided the insecticide remains effective over the entire malaria transmission season. The primary effects of IRS towards curtailing malaria transmission are : i) to reduce the life span of vector mosquitoes and ii) to reduce the density of the vector mosquitoes. Methods : CDC light trap and pyrethrum spray catches were carried out monthly to determine the change in malaria vector indices in sprayed (Diebougou) and unsprayed sites (Dano). The females malaria vectors collected by CDC light trap and PSC were used to determine their blood feeding and sporozoites rate using enzyme-linked immunosorbent assay (ELISA) and their biting rate and entomological inoculation rate Results : Three species belonging to the Anopheles gambiae complex ( Anopheles gambiae s.s . , Anopheles coluzzii and Anopheles arabiensis ) are present throughout the transmission season, but An. gambiae s.s. was the most frequent species of the complex (P =0.0005), comprising 88% of the total collected and the most infected species. Malaria vectors densities were significantly lower in sprayed villages (n=4,303) compared with unsprayed villages (n=12,569) during post-spraying period (P = 0.0012). In addition, mean human biting rate of An. gambiae sl and An . funestus ss were significantly lower in sprayed areas compared to unsprayed areas (P<0.05). Overall, malaria vector transmission risk was significant lower in villages which received IRS (P=0.0001) whatever the malaria vectors species ( An. gambiae sl and An. funestus ss). Conclusion: The results showed that in the sprayed area (Diebougou), vector densities, human biting rates and malaria transmission risks were very lower than unsprayed areas (Dano). The findings also showed a change in vector behavior especially within An. funestus which became more zoophagic following IRS. The indoor residual spraying could be recommanded as control tool in areas where malaria transmission occured throughout the year.