Miracle-dz/UniFAR-FTUs huggingface dataset:
Resourcefulness Intervention Efficacy for Parent Caregivers of Technology-Dependent Children: A Randomized Trial

Parent caregivers of children who require lifesaving technology (e.g., mechanical ventilation, feeding tubes) must maintain a high level of vigilance 24/7. A two-arm randomized controlled trial tested the efficacy of a resourcefulness intervention on parents’ mental/physical health and family functioning at four time points over six months. Participants (n = 93) cared for their technology-dependent children <18 years at home. The intervention arm received teaching on social (help-seeking), personal (self-help) resourcefulness skills; access to the intervention video and skill application video-vignettes; four weeks of skills reinforcement using daily logs; four weekly phone contacts; and booster sessions at two- and four-month postenrollment. The attention control arm received phone contact at identical time points plus the current standard of care. Statistically significant improvement was noted; fewer depressive cognitions and improved physical health for the intervention participants than attention control participants over time after controlling for covariates. The findings support the resourcefulness intervention efficacy.
Is a BTKi or BCL2i preferable for first “novel” therapy in CLL? The case for BTKis

The BTKi ibrutinib was first approved in 2014 and has completely changed the treatment paradigm in CLL/small lymphocytic lymphoma (SLL). Now, 8 years after the initial clinical studies were started, it has an established track record of durable responses. Although there were some significant toxicities that emerged, namely atrial arrhythmia and bleeding, there is now long-term experience with toxicity management. Nearly all adverse events of interest decrease in frequency over time with the 1 exception being hypertension (all grades, 11% during 0-1 years of therapy, 20% at .2-3 years of therapy). In addition, these toxicities appear to be less frequent with “next generation,” more specific BTKis. Thus, BTKis are an efficacious and tolerable choice for the majority of patients.
Cardiac rehabilitation following coronary artery dissection: recommendations and patient considerations

ABSTRACT Introduction : Cardiac rehabilitation (CR) is a multidisciplinary intervention for secondary prevention, improving functional capacity, enhancing quality of life, and improving psychosocial wellbeing in broad range of cardiovascular disease. It has been well studied over a number of years and is a Class I recommendation in multiple guidelines. However, there is a paucity of data regarding the usefulness of CR in patients with spontaneous coronary artery dissection (SCAD). Areas Covered : This narrative review aims to give an overview of the evidence underpinning CR as well as the pathophysiological mechanisms of SCAD and how they relate to exercise and shear stress. Furthermore, the evidence of the usefulness of CR in the SCAD population will be reviewed. Expert Opinion : Traditional CR programs are safe and effective in SCAD cohorts, however SCAD specific CR (SCAD-CR) has significant benefits including reductions in MACE. The principles of SCAD-CR should be applied to any CR for SCAD patients for optimal outcomes and minimization of harm.
Solving the Conundrum of Eosinophils in Alloimmunity

Eosinophils are bone-marrow-derived granulocytes known for their ability to facilitate clearance of parasitic infections and their association with asthma and other inflammatory diseases. The purpose of this review is to discuss the currently available human observational and animal experimental data linking eosinophils to the immunologic response in solid organ transplantation. First, we present observational human studies that demonstrate a link between transplantation and eosinophils yet were unable to define the exact role of this cell population. Next, we describe published experimental models and demonstrate a defined mechanistic role of eosinophils in downregulating the alloimmune response to murine lung transplants. The overall summary of this data suggests that further studies are needed to define the role of eosinophils in multiple solid organ allografts and points to the possibility of manipulating this cell population to improve graft survival.
Validation of cardiac diffusion tensor imaging sequences: A multicentre test–retest phantom study

Cardiac diffusion tensor imaging (DTI) is an emerging technique for the in vivo characterisation of myocardial microstructure, and there is a growing need for its validation and standardisation. We sought to establish the accuracy, precision, repeatability and reproducibility of state‐of‐the‐art pulse sequences for cardiac DTI among 10 centres internationally. Phantoms comprising 0%–20% polyvinylpyrrolidone (PVP) were scanned with DTI using a product pulsed gradient spin echo (PGSE; N = 10 sites) sequence, and a custom motion‐compensated spin echo (SE; N = 5) or stimulated echo acquisition mode (STEAM; N = 5) sequence suitable for cardiac DTI in vivo. A second identical scan was performed 1–9 days later, and the data were analysed centrally. The average mean diffusivities (MDs) in 0% PVP were (1.124, 1.130, 1.113) x 10−3 mm2/s for PGSE, SE and STEAM, respectively, and accurate to within 1.5% of reference data from the literature. The coefficients of variation in MDs across sites were 2.6%, 3.1% and 2.1% for PGSE, SE and STEAM, respectively, and were similar to previous studies using only PGSE. Reproducibility in MD was excellent, with mean differences in PGSE, SE and STEAM of (0.3 ± 2.3, 0.24 ± 0.95, 0.52 ± 0.58) x 10−5 mm2/s (mean ± 1.96 SD). We show that custom sequences for cardiac DTI provide accurate, precise, repeatable and reproducible measurements. Further work in anisotropic and/or deforming phantoms is warranted.
Novel method for sub‐grouping of genotype II African swine fever viruses based on the intergenic region between the A179L and A137R genes

Abstract Background African swine fever (ASF) is a highly contagious and deadly viral disease affecting domestic and wild pigs of all ages. African swine fever virus (ASFV) has spread rapidly through Eastern and Southeastern Asia first appearing in Vietnam in 2019. Objectives Molecular typing of African swine fever virus (ASFV) in Vietnam has identified two principal variants circulating based on the sequencing of the intergenic region (IRG) between the I73R and I329L genes. Identification of additional genetic markers would enable higher resolution tracing of outbreaks within the country. Methods Sequence analysis suggested the IRG between the A179L and A137R genes may also exhibit variability, PCR primers were designed and samples from Vietnam were subject to Sanger sequencing. Results We developed a novel method for sub‐grouping of ASFV based on the IRG between the A179L and A137R genes of ASFV. Our results demonstrated that the finding of the insertion or deletion of an 11‐ nucleotide sequence (GATACAATTGT) between the A179L‐A137R genes. Conclusions The sub‐grouping method may provide useful insights into the evolution of genotype II ASFV as well as providing evidence of a relationship between geographically separated outbreaks.
Alteration of the gut microbiota following SARS-CoV-2 infection correlates with disease severity in hamsters

ABSTRACT Mounting evidence suggests that the gut-to-lung axis is critical during respiratory viral infections. We herein hypothesized that disruption of gut homeostasis during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may associate with early disease outcomes. To address this question, we took advantage of the Syrian hamster model. Our data confirmed that this model recapitulates some hallmark features of the human disease in the lungs. We further showed that SARS-CoV-2 infection associated with mild intestinal inflammation, relative alteration in intestinal barrier property and liver inflammation and altered lipid metabolism. These changes occurred concomitantly with an alteration of the gut microbiota composition over the course of infection, notably characterized by a higher relative abundance of deleterious bacterial taxa such as Enterobacteriaceae and Desulfovibrionaceae. Conversely, several members of the Ruminococcaceae and Lachnospiraceae families, including bacteria known to produce the fermentative products short-chain fatty acids (SCFAs), had a reduced relative proportion compared to non-infected controls. Accordingly, infection led to a transient decrease in systemic SCFA amounts. SCFA supplementation during infection had no effect on clinical and inflammatory parameters. Lastly, a strong correlation between some gut microbiota taxa and clinical and inflammation indices of SARS-CoV-2 infection severity was evidenced. Collectively, alteration of the gut microbiota correlates with disease severity in hamsters making this experimental model valuable for the design of interventional, gut microbiota-targeted, approaches for the control of COVID-19. Abbreviations: SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; COVID-19, coronavirus disease 2019; SCFAs, short-chain fatty acids; dpi, day post-infection; RT-PCR, reverse transcription polymerase chain reaction; IL, interleukin. ACE2, angiotensin converting enzyme 2; TMPRSS2, transmembrane serine protease 2.
Examination of Student–Teacher Interpersonal Relationships Circumplex Model in the Greek Educational Context

Student–teacher interpersonal relationships contribute significantly to the academic trajectory and achievement of children and adolescents. The Questionnaire on Teacher Interaction (QTI) is one of the most widely applied measures for assessing students' perceptions about the teachers’ interpersonal behaviour. QTI comprises eight subscales that are assumed to follow a circumplex model. Prior studies on QTI’s psychometric properties are inconclusive and report mixed findings. The purpose of this study was to examine the applicability of QTI in the Greek cultural context, by testing its circumplex structure and levels of reliability. QTI was administered to 1669 secondary education students, from 85 different classrooms. A cross-validation approach and a variety of statistical techniques were employed. Subscales’ internal consistency and their ability to discriminate among classes were satisfactory. Exploratory statistical techniques provided initial support of the circular pattern. Application of a specifically designed package for testing the circumplex structure of an instrument, showed that a model in which the eight QTI subscales are placed on the circumference of a circle with equal distances form the centre was tenable. However, the assumption of equal distances was not confirmed. Deviation from the theoretical position of the subscales was mainly due to students’ difficulty to discriminate teachers’ proximity behaviour, a finding reported in various studies and across different cultural contexts. Suggestions for improving the psychometric properties of the QTI are discussed.
Decreased mobility, lack of social support, haemosiderosis and use of antidepressant medications may predict recurrent venous leg ulcers within 12 months of healing: A prospective longitudinal study

Aim To identify clinical, medical and psychosocial predictors of venous leg ulcer recurrence within 12 months of healing. Methods A multi-site study was conducted in Australia in community and hospital outpatient settings. Adults with venous leg ulcers were recruited within 4 weeks of healing and data were collected on preventative treatments and health, medical, clinical and psychosocial factors. Follow-up data on recurrences were collected every 3 months until ulcer recurrence, or until 12 months after healing pending which occurred first. Factors associated with time to recurrence were analysed using a Cox proportional hazards regression model. Design Secondary data analysis of a multi-site, prospective longitudinal study to validate a risk assessment tool for recurrence. Results A sample of 143 participants was recruited (51% male, M age = 73 years, SD 13.6). Almost half (49.6%) had an ulcer recurrence within 12 months, with a mean time to ulcer recurrence of 37 weeks (SE 1.63, 95% CI 33.7–40.1). Factors measured at the time of healing that were significant independent predictors of recurrence were: prescribed antidepressant medications (p = .035), presence of haemosiderosis (p = .006), decreased mobility (longer sitting times) (p = .007) and lower social support scale scores (p = .002). Participants who wore compression systems providing 20 mmHg or higher for at least 5 days/week were less likely to recur, although not reaching statistical significance (p = .06). Conclusion Results provide evidence that antidepressant medications, haemosiderosis, decreased mobility and lack of social support are risk factors associated with ulcer recurrence; therefore, these variables are modifiable and could guide early intervention.
AEROACOUSTIC SOURCE CHARACTERISATION USING SUBSPACE AND DECONVOLUTION TECHNIQUES

The use of phased microphone arrays has become a standard technique for aeroacoustic testing. The ultimate goal is to characterise aeroacoustic source mechanisms with respect to their location, their strength and their spectrum. A number of different analysing methods are available, ranging from classic delay-and-sum beamforming to more sophisticated methods that involve deconvolution of the beamforming map. Practical results from wind tunnel measurements using different processing techniques are presented. Both aerofoil trailing edge noise and noise from a rod-aerofoil configuration serve as examples. The processing techniques used include classic methods as well as such using the signal subspace, such using the noise subspace, the deconvolution approach for the mapping of acoustic sources (DAMAS) and a deconvolution method based on spatial source coherence (CLEAN-SC). The comparison from the results of different techniques shows significant discrepancies. This leads to the conclusion that not a single technique should be used for source characterisation, but the right one should be chosen depending on whether the location or the source strength is of primary interest.
Growth hormone-induced STAT5B regulates Star gene expression through a cooperation with cJUN in mouse MA-10 Leydig cells.

Leydig cells produce androgens that are essential for male sex differentiation and reproductive function. Leydig cell function is regulated by several hormones and signaling molecules, including growth hormone (GH). Although GH is known to upregulate Star gene expression in Leydig cells, its molecular mechanism of action remains unknown. The STAT5B transcription factor is a downstream effector of GH signaling in other systems. While STAT5B is present in both primary and Leydig cell lines, its function in these cells has yet to be ascertained. Here we report that treatment of MA-10 Leydig cells with GH or overexpression of STAT5B induces Star mRNA levels and increases steroid hormone output. The mouse Star promoter contains a consensus STAT5B element (TTCnnnGAA) at -756 bp to which STAT5B binds in vitro (EMSA and supershift) and in vivo (ChIP) in a GH-induced manner. In functional promoter assays, STAT5B was found to activate a -980 bp mouse Star reporter. Mutating the -756 bp element prevented STAT5B binding but did not abrogate STAT5B-responsiveness. STAT5B was found to functionally cooperate with DNA-bound cJUN. The STAT5B/cJUN cooperation was only observed in Leydig cells and not in Sertoli or fibroblast cells, indicating that additional Leydig cell-enriched transcription factors are required. The STAT5B/cJUN cooperation was lost only when both STAT5B and cJUN elements were mutated. In addition to identifying the Star gene as a novel target for STAT5B in Leydig cells, our data provide important new insights into the mechanism of GH and STAT5B action in the regulation of Leydig cell function.
The partition wavenumber in acoustic backscattering from a two-scale rough surface described by a power-law spectrum

Scattering from the ocean bottom is often assumed to be controlled by two spatial scales: the larger scale associated with reflections from plane facets, and the smaller one associated with diffuse scattering from height variations. Choosing the wavenumber for this partitioning has proven to be important but troublesome. For this work, scattering data are simulated using Helmholtz-Kirchhoff or physical optics theory and selected input geomorphology. These data are inverted to provide rms slope of facets and rms heights of small-scale roughness using a simple two-scale roughness model introduced previously (J. W. Caruthers and J. C. Novarini, IEEE J. Oceanic Eng., vol. 18, pp. 100-106, 1993). Bottom relief is described by power spectra of the power law form, and the bottom is assumed to be impenetrable. The work introduces a new criterion for effecting this partition based on setting a roughness parameter equal to unity. The criterion is shown to be valid for the cases analyzed based on the ability of the inversion model to recover the input geomorphology. >
Does group-based cognitive therapy improve functional ability, pain, catastrophic thoughts and quality of life in patients with persistent low back pain and psychological risk factors? A randomised controlled trial in a secondary care setting

Objective To investigate the effects of a group-based cognitive behavioural intervention for patients with persistent low back pain (LBP) and psychological risk factors referred to secondary care. Design A randomised controlled trial. Setting Silkeborg Regional Hospital, Denmark. Subjects A total of 136 participants with chronic LBP and psychological risk factors. Interventions Participants were randomised to the standard care group, including examination by a rheumatologist and/or a physiotherapist, or the intervention group, including standard care plus a multidisciplinary group-based pain management programme. Main measures Patient-reported outcomes were collected at baseline and after 6 and 12 months. The primary outcome was disability (Roland Morris Disability Questionnaire). Secondary outcomes included low back pain intensity, pain catastrophising, health-related quality of life, and sick leave. Results Among 136 participants, 68 (mean age: 41.7 years (SD 11.8)) were randomised to standard care and 68 (mean age: 46.0 years (SD 10.8)) were randomised to the intervention group. Except for age, baseline characteristics were comparable between groups. 12-month follow-up was completed by 92.6% in the intervention group and 80.9% in the standard care group. Both groups achieved significant improvements on disability, with a reduction of −4.8 points (SD 6.1) in the intervention group compared to −3.7 points (SD 5.2), resulting in an insignificant difference between groups. No significant differences were found in the secondary outcomes. Conclusion A group-based pain management programme was no more effective than standard care for patients with persistent LBP and psychological risk factors.
Porcine coronary arteries: immunohistochemical profile of TNF-alpha, IL-1beta, TGF-beta1 and ICAM-1.

In our study we used immunohistochemical technique to demonstrate the presence of the cytokines TNF-a, IL-1b, TGF-b1 and ICAM-1 in porcine coronaries even in physiological conditions. Inflammatory cytokines are polypeptide mediators which act as a communication signal between immune system cells and other types of cellsin different organs and tissues, both in human and pig coronary circulation. Our results show that pro-inflammatory cytokines TNF-a, IL-1b, TGF-b1 and ICAM-1 are also present in the medium tunica of the coronary arteries under physiological conditions. These results may be compared with those found in coronary atherosclerosis, where the increase in TNF-a has a dramatic effect on the function of the left ventricle, and the high value of IL-1 correlates directly with the extent of myocardial necrosis. In our study we observe the damage and activation of endothelial cells, this induces endothelial dysfunction by accumulation and oxidation of LDL (Low Density Lipoproteins). The formation of oxidized LDL (ox LDL) could play a central role in the amplification of the inflammatory response causing an increased expression of pro-inflammatory cytokines which promotes leukocyte recruitment in the intimal layer. These leukocytes, after the adhesion to the endothelium, penetrate the intimate tunic. Therefore inflammatory processes promote the onset and evolution of atheroma and the development of thrombotic complications.
Rapid identification of a pathogenic variant of PROS1 in a thrombophilic family by whole exome sequencing

Abstract Rationale: Venous thrombosis remains a significant problem in modern days. Genetic factors contribute to a subset of patients with venous thrombosis. It is sometimes challenging to identify the underlying culprit in thrombophilic individuals based on traditional laboratory testing and Sanger sequencing. Patient concerns: A thrombophilic family presented with multiple venous thrombosis was examined. Diagnoses: Molecular genetic analysis revealed a pathogenic missense variant of the PROS1 gene. Based on this finding and clinical manifestations, a final diagnosis of protein S deficiency was made. Interventions: Whole exome sequencing (WES) of the proband was performed to identify disease-causing variants. Subsequently, Sanger sequencing was performed to validate the variant in the affected members. Outcomes: Using WES, we rapidly identified a proven pathogenic missense variant (c.1543C > T, p.Arg515Cys) in the sex hormone-binding globulin domain of PROS1, which was confirmed by Sanger sequencing. The decreased level and activity of protein S caused by the variant explained the phenotypes of the family. Patients received rivaroxaban as a long-term anticoagulation therapy and achieved a good prognosis. Lessons: Our study suggests WES as a rapid search strategy to identify the genetic factors underlying thrombophilic disorders. Patients with venous thrombosis caused by PROS1 mutations could receive rivaroxaban as the first choice of anticoagulation therapy.
Real time planning method for multiple mobile robots

This paper proposes a motion/task planning method for multiple mobile robots. It has two characteristics as follows: (1) facility of implementing tasks, and (2) adapting ability to environment. A layered hierarchical structure is adopted for the proposed planner. The lower layer called "virtual impedance method" makes a real-time plan to follow the generated trajectory while avoiding obstacles and other robots. The upper layer determines the design parameters of the lower layer which can express "motion skills". Simulation results on motion planning and transporting tasks indicate effectiveness of the proposed method.