Miracle-dz/UniFAR-FTUs huggingface dataset:
Viral Fitness and Antigenic Determinants of Porcine Parvovirus at the Amino Acid Level of the Capsid Protein

Porcine parvovirus is one of the most important causes of reproductive failure in swine. Recently, despite the continuous use of vaccines, “new” strains emerged, leading to the hypothesis that the emergence of new amino acid substitutions could be a viral adaptation to the immune response against the commercial vaccines. ABSTRACT Since 2001, strains of porcine parvovirus (PPV), designated 27a-like strains, were observed in Europe, suggesting a predominance of these viruses over older strains. The reasons for the obvious evolutionary advantage are unknown. Here, a series of mutants containing amino acid replacements found in the predominant field strains were generated in a PPV-NADL2 background, and their impact on replication efficiency and antibody binding activity was determined. Some amino acid substitutions observed in the 27a-like strains significantly increased viral fitness and decreased neutralization activity of serum samples raised against commercial vaccines and old virus strains (e.g., NADL2). These mutant viruses and a monoclonal antibody raised against a classical PPV strain defined a 27a-specific neutralizing epitope around amino acid 228 of the capsid protein VP2. Based on the analysis of the mutant viruses, it is hypothesized that the predominant factor for the global spread of the PPV-27a strain substitutions is an increased viral fitness of the 27a-like viruses, possibly supported by partial immune selection. This is reminiscent to the evolution of canine parvovirus and worldwide replacement of the original virus by the so-called new antigenic types. IMPORTANCE Porcine parvovirus is one of the most important causes of reproductive failure in swine. Recently, despite the continuous use of vaccines, “new” strains emerged, leading to the hypothesis that the emergence of new amino acid substitutions could be a viral adaptation to the immune response against the commercial vaccines. Our results indicate the amino acid substitutions observed in the 27a-like strains can modify viral fitness and antigenicity. However, an absolute immune escape was not evident.
PERCEPTION OF DIRECT AND INDIRECT COMPENSATIONS FULFILLMENT ON HAZARDOUS WORK ENVIRONMENT: THE RELATIONSHIP WITH AGE, TENURE, EMPLOYEE’S RANK AND WORK STATUS

This paper investigates how ages, tenure, employee’s rank and work status related to perception of direct and indirect compensations fulfillment in a hazardous work environment. This study found that age, tenure, employee’s rank and work status are positively related to perception of direct and indirect compensations fulfillment. Implication for research and practice are discussed. Keywords: Demographic characteristics, direct and indirect compensations, Hazardous work environment
Design and modeling of a Laue lens for radiation therapy with hard x-ray photons

We have designed and modeled a novel optical system composed of a Laue lens coupled to an x-ray tube that produces a focused beam in an energy range near 100 keV (λ = 12.4 picometer). One application of this system is radiation therapy where it could enable treatment units that are considerably simpler and lower in cost than present technologies relying on linear accelerators. The Laue lens is made of Silicon Laue components which exploit the silicon pore optics technology. The lens concentrates photons to a small region thus allowing high dose rates at the focal area with very much lower dose rates at the skin and superficial regions. Monte Carlo simulations with Geant4 indicate a dose deposition rate of 0.2 Gy min−1 in a cylindrical volume of 0.7 mm diameter and 10 mm length, and a dose ratio of 72 at the surface (skin) compared to the focus placed 10 cm within a water phantom. Work is ongoing to newer generation crystal technologies to increase dose rate.
[Xingnao Kaiqiao acupuncture on promoting wake-up of vegetative state after brain injury].

OBJECTIVE To observe the clinical effect of Xingnao Kaiqiao acupuncture on promoting wake-up of vegetative state after brain injury on the basis of comprehensive rehabilitation training. METHODS A total of 100 patients with vegetative state after brain injury were randomly divided into an observation group (50 cases, 2 cases dropped off) and a control group (50 cases). Both groups were treated with routine clinical treatment. The patients in the control group were treated with rehabilitation and hyperbaric oxygen; on the basis of the control group treatment, the patients in the observation group were treated with Xingnao Kaiqiao acupuncture at Neiguan (PC 6), Shuigou (GV 26), Sanyinjiao (SP 6), Chize (LU 5), Weizhong (BL 40), Hegu (LI 4) and Taichong (LR 3). The acupuncture was given once a day, 5 days per week, for continuous 30 days. The Glasgow coma scale (GCS) and coma recovery scale-revised (CRS-R) scores were observed before treatment and 10, 20 and 30 days into treatment. The wake-up rate of the two groups was compared after treatment. RESULTS On 10, 20 and 30 days into treatment, the GCS and CRS-R scores in the two groups were higher than those before treatment (P<0.01), and the scores in the observation group were higher than those in the control group (P<0.01). After treatment, the wake-up rate was 16.7% (8/48) in the observation group, which was higher than 12.0% (6/50) in the control group (P<0.01). CONCLUSION On the basis of comprehensive rehabilitation and wake-up promotion therapy, the Xingnao Kaiqiao acupuncture could promote the recovery of consciousness level in patients with vegetative state after brain injury.
Suicidal Thoughts and Behaviors Among Adolescent Psychiatric Inpatients

Abstract Background Given low base rates of suicidal thoughts and behaviors (STBs) in national samples of adolescents, clarifying the sociodemographic and clinical correlates among psychiatric inpatients may afford insights into potential risk factors that predict STBs onset. Method Adolescents (N = 970; ages 12–19 years) admitted for acute, psychiatric inpatient care completed baseline clinical interviews and self-report measures assessing demographics and early life adversity. Lifetime and 12-month STBs prevalence were obtained, allowing for the estimate of STBs persistence (i.e., rates of those with both current and past STBs) and transition rates (i.e., proportion of ideators that transition to plans or attempts). Univariate and multivariate logistic regression tested sociodemographic and clinical correlates of STBs. Results Age-of-onset for STBs occurred in early adolescence. Most patients reported suicide ideation with nearly half of patients making a plan and one-third a suicide attempt. Although relatively modest, the strongest correlates of lifetime attempts were depressive disorders, physical abuse, and non-suicidal self-injury. Knowing a peer that had attempted suicide also increased the likelihood of a suicide attempt, especially among attempters who transitioned from ideation to planned attempts. Conclusion STBs are highly prevalent among adolescents admitted for acute psychiatric inpatient treatment. The modest effects suggest that correlates, particularly those related to suicide attempts, are widely distributed. As a history of physical abuse and knowing a peer with a suicide attempt history are related to transitioning from ideation to action, these may be critical factors to target in the deployment of future suicide prevention and treatment programs. HIGHLIGHTS One-third of adolescent inpatients report a lifetime history of suicide attempts. Approximately 65% of adolescent inpatients with a lifetime plan attempt suicide. Knowing peers who attempt suicide may facilitate the transition from ideation to action.
The use of late time response for stand off onbody concealed weapon detection

A new system for remote detection of onbody concealed weapons such as knives and handheld guns at standoff distances presented in this thesis. The system was designed, simulated, constructed and tested in the laboratory. The detection system uses an Ultrawide Band (UWB) antenna to bombard the target with a UWB electromagnetic pulse. This incident pulse induces electrical currents in the surface of an object such as a knife, which given appropriate conditions these currents generate an electromagnetic backscatter radiation. The radiated waves are detected using another UWB antenna to obtain the Late Time Response (LTR) signature of the detected object. The LTR signature was analysed using the Continuous Wavelet Transform (CWT) in order to assess the nature and the geometry of the object. The thesis presents the work which divided into two related areas. The first involved the design, simulation, fabrication, and testing of an Ultra-wide Band (UWB) antenna with operating bandwidth of 0.25 – 3.0 GHz and specific characteristics. Simulated and measured results show that the designed antenna achieves the design objectives which are, flat gain, a VSWR of around unity and distortion less transmitted narrow pulse. The operating bandwidth was chosen to cover the fundamental Complex Natural Resonance (CNR) modes of most firearms and to give a fine enough time resolution. The second area covered by this thesis presents a new approach for extract target signature based on the Continuous Wavelet Transform (CWT) applied to the scattering response of onbody concealed weapons. A series of experiments were conducted to test the operation of the detection system which involved onbody and offbody objects such as, knives, handheld guns, and a number of metallic wires of various dimensions. Practical and simulation results were in good agreement demonstrating the success of the approach of using the CWT in analyzing the LTR signature which is used for the first time in this work. Spectral response for every target could be seen as a distribution in which the energy level and life-time depended on the target material and geometry. The spectral density provides very powerful information concerning target unique signature.
Keeping histology students under the microscope during Covid‐19 lockdown: Lessons learned from the University of Genoa

We have read, with great interest, articles published recently in Anatomical Sciences Education that describe histology education during the Covid-19 pandemic (Evans et al., 2020; Caruso, 2021; Cheng et al., 2021a, b; Somera et al., 2021). As histology educators at the University of Genoa, we would like to add our perspective to these conversations.
Correlating genotype with phenotype using CFTR‐mediated whole‐cell Cl− currents in human nasal epithelial cells

Dysfunction of the epithelial anion channel cystic fibrosis transmembrane conductance regulator (CFTR) causes a wide spectrum of disease, including cystic fibrosis (CF) and CFTR‐related diseases (CFTR‐RDs). Here, we investigate genotype–phenotype–CFTR function relationships using human nasal epithelial (hNE) cells from a small cohort of non‐CF subjects and individuals with CF and CFTR‐RDs and genotypes associated with either residual or minimal CFTR function using electrophysiological techniques. Collected hNE cells were either studied directly with the whole‐cell patch‐clamp technique or grown as primary cultures at an air–liquid interface after conditional reprogramming. The properties of cAMP‐activated whole‐cell Cl− currents in freshly isolated hNE cells identified them as CFTR‐mediated. Their magnitude varied between hNE cells from individuals within the same genotype and decreased in the rank order: non‐CF > CFTR residual function > CFTR minimal function. CFTR‐mediated whole‐cell Cl− currents in hNE cells isolated from fully differentiated primary cultures were identical to those in freshly isolated hNE cells in both magnitude and behaviour, demonstrating that conditional reprogramming culture is without effect on CFTR expression and function. For the cohort of subjects studied, CFTR‐mediated whole‐cell Cl− currents in hNE cells correlated well with CFTR‐mediated transepithelial Cl− currents measured in vitro with the Ussing chamber technique, but not with those determined in vivo with the nasal potential difference assay. Nevertheless, they did correlate with the sweat Cl− concentration of study subjects. Thus, this study highlights the complexity of genotype–phenotype–CFTR function relationships, but emphasises the value of conditionally reprogrammed hNE cells in CFTR research and therapeutic testing.
Clinical Indoor Running Gait Analysis May Not Approximate Outdoor Running Gait Based on Novel Drone Technology

Background: Traditional running gait analysis is limited to artificial environments, but whether treadmill running approximates overground running is debated. This study aimed to compare treadmill gait analysis using fixed video with outdoor gait analysis using drone video capture. Hypothesis: Measured kinematics would be similar between natural outdoor running and traditional treadmill gait analysis. Study Design: Crossover study. Level of Evidence: Level 2. Methods: The study population included cross-country, track and field, and recreational athletes with current running mileage of at least 15 km per week. Participants completed segments in indoor and outdoor environments. Indoor running was completed on a treadmill with static video capture, and outdoor segments were obtained via drone on an outdoor track. Three reviewers independently performed clinical gait analysis on footage for 32 runners using kinematic measurements with published acceptable intra- and interrater reliability. Results: Of the 8 kinematic variables measured, 2 were found to have moderate agreement indoor versus outdoor, while 6 had fair to poor agreement. Foot strike at initial contact and rearfoot position at midstance had moderate agreement indoor versus outdoor, with a kappa of 0.54 and 0.49, respectively. The remaining variables: tibial inclination at initial contact, knee flexion angle initial contact, forward trunk lean full gait cycle, knee center position midstance, knee separation midstance, and lateral pelvic drop at midstance were found to have fair to poor agreement, ranging from 0.21 to 0.36. Conclusion: This study suggests that kinematics may differ between natural outdoor running and traditional treadmill gait analysis. Clinical Relevance: Providing recommendations for altering gait based on treadmill gait analysis may prove to be harmful if treadmill analysis does not approximate natural running environments. Drone technology could provide advancement in clinical running recommendations by capturing runners in natural environments.
Regulation of positive and negative emotions across cultures: does culture moderate associations between emotion regulation and mental health?

ABSTRACT Emotion regulation (ER) has been frequently linked to mental health (MH) with previous research focussing on a limited range of ER strategies. The present study examined whether strategies for the regulation of positive and negative emotions are differently related to MH (i.e. higher subjective well-being, less depressive symptoms) across cultures. Two samples consisting of 524 Japanese and 476 German-speaking (“Germans”) university students completed our survey. Moderation analyses revealed cultural similarities and differences in associations between ER and MH. Across cultures, distraction from negative emotions, savouring positive emotions, and reappraisal were related to better MH, whereas distraction from positive emotions and ruminating on negative experiences were related to worse MH. Moreover, the link between rumination and lower well-being was significantly weaker among Japanese compared to Germans. Expressive suppression was related to lower MH among Germans only. Contrary to the German pattern, suppressing negative emotions out of empathic concern was associated with better MH among Japanese, which was mediated by interdependent self-construal. Our findings suggest that masking negative emotions out of concern for others might be an adaptive strategy for Japanese by reinforcing interdependent values. We highlight the role of culture and the importance of distinguishing different ER strategies and emotion types.
Anti-tuberculosis drug delivery for tuberculous bone defects

ABSTRACT Introduction Traditional therapy methods for treating tuberculous bone defects have several limitations. Furthermore, systemic toxicity and disease recurrence in tuberculosis (TB) have not been effectively addressed. Areas covered This review is based on references from September 1998 to September 2021 and summarizes the classification and drug-loading methods of anti-TB drugs. The application of different types of biological scaffolds loaded with anti-TB drugs as a novel drug delivery strategy for tuberculous bone defects has been deeply analyzed. Furthermore, the limitations of the existing studies are summarized. Expert opinion Loading anti-TB drugs into the scaffold through various drug-loading techniques can effectively improve the efficiency of anti-TB treatment and provide an effective means of treating tuberculous bone defects. This methodology also has good application prospects and provides directions for future research.
[Therapeutic effect of electric-balance stimulation with scalp acupuncture for motor aphasia after cerebral infarction].

OBJECTIVE To compare the clinical efficacy between scalp acupuncture electrical stimulation and routine scalp acupuncture for motor aphasia in subacute stage of cerebral infarction. METHODS A total of 54 patients with motor aphasia in subacute stage of cerebral infarction were randomly divided into an observation group (27 cases, 1 case dropped off) and a control group (27 cases, 2 cases dropped off ). Both groups were treated with routine medication and language training. In the observation group, scalp acupuncture was given at bilateral lower 2/5 of the parietal and temporal anterior oblique line and temporal frontline; after the arrival of qi, the electrical stimulation with disperse-dense wave was given at the affected side and continuous wave was given at healthy side. The control group was treated with routine scalp acupuncture at lower 2/5 of the parietal and temporal anterior oblique line and temporal frontline of the affected side, once a day, five times as a course, totaling two courses of treatment. The aphasia battery of Chinese (ABC) score and Boston diagnostic aphasia examination (BDAE) grade were observed before and after treatment. The levels of oxygenated hemoglobin (HbO2), deoxyhemoglobin (D-Hb) and total hemoglobin (T-Hb) in local cerebral cortex of the two groups were measured in real time using functional near-infrared spectroscopy (fNIRS) before and after treatment. The clinical efficacy of the two groups was compared. RESULTS After treatment, the scores of listening comprehension, retelling, naming, spontaneous conversation and BDAE grade in the two groups were improved compared with those before treatment (P<0.01, P<0.05), and those in the observation group were better than the control group (P<0.05). Compared before treatment, the levels of HbO2 and T-Hb were increased (P<0.01), and the levels of D-Hb were decreased (P<0.01) after treatment in the two groups. The levels of HbO2 and T-Hb in the observation group were higher than those in the control group (P<0.05), and the level of D-Hb was lower than that in the control group (P<0.05). The total effective rate was 92.3% (24/26) in the observation group, which was higher than 84.0% (21/25) in the control group (P<0.05). CONCLUSION The scalp acupuncture electrical stimulation could improve cerebral circulation, activate specific functional areas of cerebral cortex, and promote the reconstruction and recovery of brain language function. Its curative effect is better than conventional scalp acupuncture.
Cell loss recovery in Banyan networks using RS codes under self-similar traffic

In order to cater the needs of the users in the communication field in terms of high speed, high bandwidth, error free reception, researchers focused their attention towards BISDN/ATM in the recent years. Since the major efficiency of the ATM networks depends on the ATM switching, in this paper, we focused our attention towards high throughput of an ATM switch architecture. The Banyan switch architecture is taken for our study in this paper. We have simulated an 8 X 8 Banyan architecture with the proposed scheme of using RS coder and decoder. The Banyan architecture has been studied for its performance with Self-Similar traffic. The performance of the proposed architecture in terms of cell loss and average delay versus the cell arrival rate is presented.
Expanding the phenotypic and allelic spectrum of SMG8: Clinical observations reveal overlap with SMG9‐associated disease trait

SMG8 (MIM *617315) is a regulatory subunit involved in nonsense‐mediated mRNA decay (NMD), a cellular protective pathway that regulates mRNA transcription, transcript stability, and degrades transcripts containing premature stop codons. SMG8 binds SMG9 and SMG1 to form the SMG1C complex and inhibit the kinase activity of SMG1. Biallelic deleterious variants in SMG9 are known to cause a heart and brain malformation syndrome (HBMS; MIM #616920), whereas biallelic deleterious variants in SMG8 were recently described to cause a novel neurodevelopmental disorder (NDD) with dysmorphic facies and cataracts, now defined as Alzahrani–Kuwahara syndrome (ALKUS: MIM #619268). Only eight subjects from four families with ALKUS have been described to date. Through research reanalysis of a nondiagnostic clinical exome, we identified a subject from a fifth unrelated family with a homozygous deleterious variant in SMG8 and features consistent with ALKUS. Interestingly, the subject also had unilateral microphthalmia, a clinical feature that has been described in SMG9‐related disorder. Our study expands the phenotypic spectrum of SMG8‐related disorder, demonstrates an overlapping phenotype between SMG8‐ and SMG9‐related rare disease traits, provides further evidence for the SMG8 and SMG9 protein interactions, and highlights the importance of revisiting nondiagnostic exome data to identify and affirm emerging novel genes for rare disease traits.