Miracle-dz/UniFAR-FTUs huggingface dataset:
Obese mice on a high‐fat alternate‐day fasting regimen lose weight and improve glucose tolerance

Alternate-day fasting (ADF) causes body weight (BW) loss in humans and rodents. However, it is not clear that ADF while maintaining a high-fat (HF) diet results in weight loss and the accompanying improvement in control of circulating glucose. We tested the hypotheses that a high-fat ADF protocol in obese mice would result in (i) BW loss, (ii) improved glucose control, (iii) fluctuating phenotypes on 'fasted' days when compared to 'fed' days and (iv) induction of torpor on 'fasted days'. We evaluated the physiological effects of ADF in diet-induced obese mice for BW, heart rate (HR), body temperature (Tb ), glucose tolerance, insulin responsiveness, blood parameters (leptin, insulin, free fatty acids) and hepatic gene expression. Diet-induced obese male C57BL/6J mice lost one-third of their pre-diet BW while on an ADF diet for 10 weeks consisting of HF food. The ADF protocol improved glucose tolerance and insulin sensitivity, although mice on a fast day were less glucose tolerant than the same mice on a fed day. ADF mice on a fast day had low circulating insulin, but had an enhanced response to an insulin-assisted glucose tolerance test, suggesting the impaired glucose tolerance may be a result of insufficient insulin production. On fed days, ADF mice were the warmest, had a high HR and displayed hepatic gene expression and circulating leptin that closely mimicked that of mice fed an ad lib HF diet. ADF mice never entered torpor as assessed by HR and Tb . However, on fast days, they were the coolest, had the slowest HR, and displayed hepatic gene expression and circulating leptin that closely mimicked that of Chow-Fed mice. Collectively, the ADF regimen with a HF diet in obese mice results in weight loss, improved blood glucose control, and daily fluctuations in selected physiological and biochemical parameters in the mouse.
Observations on Anti‐Human Globulin Sera

WHEN the anti-human globulin test was introduced into clinical work it was at first used for the detection of blood-group antibodies, particularly Rh antibodies. It was shown that the iiicoinplete Rh antibody was mostly, if not entirely, in the gamma-globulin fraction of serum (Coombs and Mourant, 1947). The use of the antiglobulin test was soon extended to thc diagnosis of acquired haemolytic anacmia. Dacie (1951) showed that an antiglobulin serum neutralized with small amounts of gamma globulin failed to react with Rh-sensitized cells or with cells from patients suffering from acquired haemolytic anaemia of thc ‘warni-antibody type’; such a serum would, howcvcr, react with cells sensitized by normal cold antibodies or with cells from patients with acquired haemolytic anaemia of the ‘cold antibody type,. He concluded that the antibodies of the warm-antibody type of acquired haemolytic anaemia appeared to be gamma globuliiis whereas those of the cold-antibody type might not be. Differences between the types of globulin on sensitized red cells have also been demonstrated by cross-absorption experiments; for example, it has been shown that an antiglobulin scrum absorbed with cells sensitized with Rh antibody will still react with cells sensitized with normal incomplete cold antibody (Crawford and Mollison, 195 I). The present payer describes further cross-absorption experiments, together with the results of testing sensitized cclls with antiglobulin sera of known composition.
Probabilistic qos analysis in wireless sensor networks

Emerging applications of wireless sensor networks (WSNs) require real-time quality of service (QoS) guarantees to be provided by the network. Traditional analysis work only focuses on the first-order statistics, such as the mean and the variance of the QoS performance. However, due to unique characteristics of WSNs, a cross-layer probabilistic analysis of QoS performance is essential. In this dissertation, a comprehensive cross-layer probabilistic analysis framework is developed to investigate the probabilistic evaluation and optimization of QoS performance provided by WSNs. In this framework, the distributions of QoS performance metrics are derived, which are natural tools to discover the probabilities to achieve given QoS requirements. Compared to first-order statistics, the distribution of these metrics reveals the relationship between the performance of QoS-based operations and the probability to achieve the performance. Using a Discrete-Time Markov queueing model in node-level analysis and fluid models in network-level analysis, the distributions of end-to-end delay, the network lifetime, and the event detection delay are then analyzed. Based on the evaluation of QoS metrics, a probabilistic optimization framework is developed to demonstrate the investigation of the optimal network and protocol parameters. Guidelines of designing networks and choosing optimal parameters for WSNs are provided using the optimization framework. Intensive testbed experiments and simulations are used to validate the accuracy of the proposed evaluation and optimization framework.
Application of Power Source Element in Power Factor Correction

A new application of power source element in power factor correction is proposed. The power source is inserted between the bridge rectifier diodes and a large bulk capacitor to increase the conduction angle of the rectifier diodes. The power from the DC bus is transferred to power the source via a loss free resistor connected with the DC bus. Analysis, design example, control scheme, and simulation results are given in this paper.
The incidence and severity of dental trauma in intercollegiate athletes.

BACKGROUND The use of protective devices such as mouthguards during participation in contact sports may be effective in reducing the incidence or severity of dental injuries. METHODS Dental injuries reported to the athletic department at the University of Southern California, Los Angeles, were recorded from 1996 through 2005. The authors classified each injury and determined the severity of the injury. Severity was defined in relation to the treatment required and the prognosis of the teeth and supporting tissues involved. RESULTS Fifty-one traumatic dental injuries were reported. Basketball was the sport with the highest injury rate; it had an incidence rate (IR) of 10.6 injuries per 100 athlete-seasons among men, and an IR of 5.0 injuries per 100 athlete-seasons among women. The IR for men's basketball players was five times higher than that for football players for whom mouthguard use is mandatory. CONCLUSIONS Given the relatively high incidence of dental injury in basketball and the potential of mouthguard use to reduce the incidence and severity of the trauma, mandatory use of mouthguards among collegiate basketball players should be considered. CLINICAL IMPLICATIONS Dental professionals have a responsibility to educate patients and the public about the importance of using mouth-guard protection in contact sports.
Fibrinogen, Factor VII and PAI-1 Genotypes and the Risk of Coronary and Peripheral Atherosclerosis: Edinburgh Artery Study

Summary There are now many epidemiological studies that have shown a relationship between haemostatic factors and subsequent risk of both coronary and peripheral arterial disease. However, there is less information on the association between genetic variation in these factors and the risks of coronary and peripheral arterial disease. As part of the five-year follow-up of the Edinburgh Artery Study, polymorphisms of the fibrinogen (-455G/A), factor VII (R/Q353) and PAI-1 (HindIII) genes were measured in men and women aged 60-79 years, together with their plasma levels. Using widely accepted criteria, 88 subjects were identified as having peripheral arterial disease (PAD), 195 having coronary artery disease (CAD) and 423 subjects comprised a “healthy” group. The -455AA genotype of the fibrinogen gene was found to be more frequent among those subjects with PAD. This genotype also showed the highest plasma fibrinogen levels in both disease groups and in the healthy group. Using logistic regression, after adjustment for age, sex, smoking and plasma level, the -455AA genotype was associated with over twice the risk of PAD compared with the -455GG genotype, the odds ratio reaching marginal significance (p″0.10). Combining those with genotype -455AA with the heterozygotes in order to increase the power of the study resulted in a more significant multiple-adjusted risk of PAD (p″0.05). These data provide evidence that a polymorphism of the β fibrinogen gene is associated with an increased risk of peripheral atherosclerosis.
Enhanced sensitivity to hydrogen peroxide‐induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III

EVI1 is a nuclear zinc finger protein essential to normal development, which participates in acute myeloid leukaemia progression and transforms Rat1 fibroblasts. In this study we show that enforced expression of Evi1 in Rat1 fibroblasts protects from paclitaxel‐induced apoptosis, consistent with previously published studies. Surprisingly, however, these cells show increased sensitivity to hydrogen peroxide (H2O2)‐induced apoptosis, demonstrated by elevated caspase 3 catalytic activity. This effect is caused by a reduction in carbonic anhydrase III (caIII) production. caIII transcripts are repressed by 92–97% by Evi1 expression, accompanied by a similar reduction in caIII protein. Reporter assays with the rat caIII gene promoter show repressed activity, demonstrating that Evi1 either directly or indirectly modulates transcription of this gene in Rat1 cells. Targeted knockdown of caIII alone, with Dicer‐substrate short inhibitory RNAs, also increases the sensitivity of Rat1 fibroblasts to H2O2, which occurs in the absence of any other changes mediated by Evi1 expression. Enforced expression of caIII in Evi1‐expressing Rat1 cells reverts the phenotype, restoring H2O2 resistance. Together these data show that Evi1 represses transcription of caIII gene expression, leading to increased sensitivity to H2O2‐induced apoptosis in Rat1 cells and might suggest the basis for the development of a novel therapeutic strategy for the treatment of leukaemias and solid tumours where EVI1 is overexpressed.
Multinational study of the efficacy and safety of humanized anti-HER2 monoclonal antibody in women who have HER2-overexpressing metastatic breast cancer that has progressed after chemotherapy for metastatic disease.

PURPOSE Overexpression of the HER2 protein occurs in 25% to 30% of human breast cancers and leads to a particularly aggressive form of the disease. Efficacy and safety of recombinant humanized anti-HER2 monoclonal antibody as a single agent was evaluated in women with HER2-overexpressing metastatic breast cancer that had progressed after chemotherapy for metastatic disease. PATIENTS AND METHODS Two hundred twenty-two women, with HER2-overexpressing metastatic breast cancer that had progressed after one or two chemotherapy regimens, were enrolled. Patients received a loading dose of 4 mg/kg intravenously, followed by a 2-mg/kg maintenance dose at weekly intervals. RESULTS Study patients had advanced metastatic disease and had received extensive prior therapy. A blinded, independent response evaluation committee identified eight complete and 26 partial responses, for an objective response rate of 15% in the intent-to-treat population (95% confidence interval, 11% to 21%). The median duration of response was 9.1 months; the median duration of survival was 13 months. The most common adverse events, which occurred in approximately 40% of patients, were infusion-associated fever and/or chills that usually occurred only during the first infusion, and were of mild to moderate severity. These symptoms were treated successfully with acetaminophen and/or diphenhydramine. The most clinically significant adverse event was cardiac dysfunction, which occurred in 4.7% of patients. Only 1% of patients discontinued the study because of treatment-related adverse events. CONCLUSION Recombinant humanized anti-HER2 monoclonal antibody, administered as a single agent, produces durable objective responses and is well tolerated by women with HER2-overexpressing metastatic breast cancer that has progressed after chemotherapy for metastatic disease. Side effects that are commonly observed with chemotherapy, such as alopecia, mucositis, and neutropenia, are rarely seen.
Albumin restores lysophosphatidylcholine-induced inhibition of vasodilation in rat aorta.

BACKGROUND Impairment of vasodilation by oxidized low-density lipoprotein has been attributed to lysophosphatidylcholine (LPC). Albumin avidly binds LPC. Therefore, hypoalbuminemia may directly impair vasodilation and thus contribute to increased risk of atherosclerosis in nephrotic syndrome. The addition of albumin reduces LPC in erythrocytes and endothelial cells. We hypothesized that the addition of albumin will salvage vasodilation in aortic rings previously exposed to LPC. LPC increases superoxide production and disturbs L-arginine availability. Therefore, we also decreased superoxide with a superoxide dismutase mimic, MnCl(2), and supplemented L-arginine in an attempt to restore vasodilation. METHODS Rat aorta rings, which had been incubated with various concentrations of LPC and human serum albumin (HSA), were mounted in organ chambers. Relaxation was studied with acetylcholine (0.01 to 100 micromol/L) after precontraction with phenylephrine (CON, 0.3 micromol/L; LPC, 0.03 micromol/L). In some studies MnCl(2) or L-arginine was added to the organ chamber. RESULTS LPC had time- and dose-dependent inhibitory effects on acetylcholine-mediated vasodilation, but no effect on nitroprusside-mediated vasodilation. Preincubation with albumin (50 or 6 g/L) could protect vasodilation against very high levels of LPC. After preincubation with LPC, the addition of albumin to the incubation salvaged vasodilation. Albumin was more effective after short LPC incubation. MnCl(2) had no specific effect on the LPC-mediated disturbance in vasodilation. L-arginine completely salvaged vasodilation at low concentrations of LPC. However, even high concentrations of L-arginine (1 mmol/L) could not improve vasodilation at LPC levels at which vasodilation was restored by albumin. CONCLUSIONS LPC affects several pathways that inhibit vasodilation, all of which are salvaged by addition of albumin.
An analysis of AC conductivity in moist oil-impregnated insulation pressboard

The paper presents research results of testing AC conductivity in a moist oilimpregnated electric insulation pressboard, depending on the moisture content level and temperature of the samples. A new method has been elaborated for converting experimentally obtained dependences from frequency-domain spectroscopy (FDS) to the reference temperature of 293 K (20 /dpl deg/C). Activation energy values have been determined for the conduction and relaxation time. It has been found the conductivity is realized by electron tunnelling between potential well formed by water molecules contained in the structure of moist cellulose.
Development of the optimal inoculation conditions for microcarrier cultures

The environmental conditions under which anchorage‐dependent mammalian cells are grown are not necessarily those under which a culture should be initiated. Cell attachment is a physical process, and those factors which affect forces involved in cell attachment differ from the biological factors which affect cell growth. We have conducted an extensive experimental study to define clearly the optimal environmental conditions for MRC‐5 cell attachment onto microcarriers. These inoculation conditions are particularly important when the serial propagation of mammalian cells on microcarriers is considered as in a human vaccine production process. The conditions which were investigated are: initial serum content (% v/v), initial pH, inoculation level (cells/bead), agitation rate (rpm), and the concentration of microcarriers (g/L). The initial distribution of attached cells was found to have a significant affect on the overall efficiency of anchorage‐dependent cell cultures, and was used to evaluate attachment efficiency. Based on the experimental results, we propose an optimized protocol for the inoculation of microcarrier cultures.
The long‐term trend in the diurnal temperature range over Asia and its natural and anthropogenic causes

Understanding the causes of long‐term temperature trends is at the core of climate change studies. Any observed trend can result from natural variability or anthropogenic influences or both. In the present study, we evaluated the performance of 18 climate models from the Coupled Model Intercomparison Project Phase 5 on simulating the Asian diurnal temperature range (DTR) and explored the potential causes of the long‐term trend in the DTR by examining the response of the DTR to natural forcing (volcanic aerosols and solar variability) and anthropogenic forcing (anthropogenic greenhouse gases (GHG) and aerosols) in the historical period of 1961–2005. For the climatology, the multimodel ensemble mean reproduced the geographical distribution and amplitude of the DTR over eastern China and India but underestimated the magnitudes of the DTR over the Tibetan Plateau and the high‐latitude regions of the Asian continent. These negative biases in the DTR over frigid zones existed in most models. Seasonal biases in the DTR pattern from models were similar to the bias in the annual mean DTR pattern. Based on three selected state‐of‐the‐art models, the observed decreasing trend in the DTR over Asia was reasonably reproduced in the all‐forcing run. A comparison of separate forcing experiments revealed that anthropogenic forcing plays the dominant role in the declining trend in the DTR. Observations and model simulations showed that GHG forcing is mainly responsible for the negative trends in the DTR over Asia but that anthropogenic aerosol forcing was also behind the decreasing trend in the DTR over China and especially over eastern China.
Acute effects of insulin-like growth factor I on glucose and amino acid metabolism in the awake fasted rat. Comparison with insulin.

To elucidate the acute metabolic actions of insulin-like growth factor I (IGF-I), we administered a primed (250 micrograms/kg), continuous (5 micrograms/kg.min) infusion of human recombinant (Thr 59) IGF-I or saline to awake, chronically catheterized 24-h fasted rats for 90 min. IGF-I was also infused while maintaining euglycemia (glucose clamp technique) and its effects were compared to those of insulin. IGF-I infusion caused a twofold rise in IGF-I levels and a 75-85% decrease in plasma insulin. When IGF-I alone was given, plasma glucose fell by 30-40 mg/dl (P less than 0.005) due to a transient twofold increase (P less than 0.05) in glucose uptake; hepatic glucose production and plasma FFA levels remained unchanged. IGF-I infusion with maintenance of euglycemia produced a sustained rise in glucose uptake and a marked stimulation of [3-3H]glucose incorporation into tissue glycogen, but still failed to suppress glucose production and FFA levels. IGF-I also produced a generalized 30-40% reduction in plasma amino acids, regardless of whether or not hypoglycemia was prevented. This was associated with a decrease in leucine flux and a decline in the incorporation of [1-14C]leucine into muscle and liver protein (P less than 0.05). When insulin was infused in a dosage that mimicked the rise in glucose uptake seen with IGF-I, nearly identical changes in amino acid metabolism occurred. However, insulin suppressed glucose production by 65% and FFA levels by 40% (P less than 0.001). Furthermore, insulin was less effective than IGF-I in promoting glycogen synthesis. We conclude that (a) IGF-I produces hypoglycemia by selectively enhancing glucose uptake; (b) IGF-I is relatively ineffective in suppressing hepatic glucose production or FFA levels; and (c) IGF-I, like insulin, lowers circulating amino acids by reducing protein breakdown rather than by stimulating protein synthesis. Thus, IGF-I's metabolic actions in fasted rats are readily distinguished from insulin.
Polymorphisms TaqI A of the DRD2, BalI of the DRD3, exon III repeat of the DRD4, and 3′ UTR VNTR of the DAT: Association with childhood ADHD in male African‐Caribbean cocaine dependents?

The conflicting results reported by genetic studies with the variants of the genes coding for the dopaminergic system in cocaine addicts could be partially explained by the difficulties to constitute homogenous sample of patients. Childhood attention‐deficit/hyperactivity disorder (ADHD), and/or impulsivity are frequently associated with cocaine addiction and could participate in the heterogeneity of the samples in cocaine addicts. Accordingly, it is hypothesized that cocaine addiction would be associated with the variants of the genes coding for the dopamine system in an homogenized sample of cocaine addicts, especially in individuals with childhood ADHD comorbidity, or with a high impulsivity score. The potential association of the variants TaqI A of the DRD2, BalI of the DRD3, exon III repeat of the DRD4, and 3′ UTR VNTR of the DAT was examined in African‐Caribbean males, smoked‐cocaine dependents. All the subjects were assessed with the Diagnostic Interview of Genetic Studies, the Barratt's impulsivity scale, and the Wender Utah rating scale for childhood ADHD. A positive association was found with the DRD2 and DRD4 polymorphisms in the subgroups of patients with childhood ADHD, or with a high impulsivity score, which represented, respectively, 53.3 and 73.0% of the patients. Conversely, no positive association was found for any of the polymorphisms studied when the group of patients was examined as a whole. Therefore, our results suggest that the clinical dimensions of childhood ADHD and of impulsivity could be taken into account to homogenize the samples of patients in cocaine association studies. © 2007 Wiley‐Liss, Inc.
Political Survival and Endogenous Institutional Change

Incumbent political leaders risk deposition by challengers within the existing political rules and by revolutionary threats. Building on Bueno de Mesquita, Smith, Siverson, and Morrow's selectorate theory, the model here examines the policy responses of office-seeking leaders to revolutionary threats. Whether leaders suppress public goods such as freedom of assembly and freedom of information to hinder the organizational ability of potential revolutionaries or appease potential revolutionaries by increasing the provision of public goods depends, in part, on the sources of government revenues. Empirical tests show that governments with access to revenue sources that require few labor inputs by the citizens, such as natural resource rents or foreign aid, reduce the provision of public goods and increase the odds of increased authoritarianism in the face of revolutionary pressures. In contrast, without these sources of unearned revenues, governments respond to revolutionary pressures by increasing the provision of public goods and democratizing.