Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Danxuan Koukang on Proliferation and PCNA Production of Human Oral Mucosal Fibroblasts Induced by Areca Nut Extract

Objective To study the effect of Danxuan Koukang(DXKK) drug serum on proliferation and proliferating cell nuclear antigen(PCNA) production of human oral mucosal fibroblasts(FB) induced by areca nut extract(ANE). Methods Human oral mucosal FB were cultured in vitro,100 μg/mL ANE was used as inducement and 10% DXKK drug serum as intervenor.The cell proliferation rates were measured by MTT assay,PCNA productions were examined by immunocytechemistry.Results MTT assay showed OD value in DXKK groups were significantly decreased compared with the induced group(P 0.01 or P 0.05). Immunocytechemistry showed counts and relative grey value of PCNA–positive cells in DXKK groups were significantly decreased compared with the induced group(P0.01 or P 0.05). Conclusion DXKK can inhibit proliferation and PCNA production of human oral mucosal FB induced by ANE.
Electrical Penetration Graphs of Adelphocoris suturalis Jakovlev in Main Host Crops

【Objective】 The feeding behavior of Adelphocoris suturalis was investigated in the laboratory in order to provide basis for evaluation of host plant resistance and host choice of this insect. 【Method】 The probing waveforms of A. suturalis on green bean, alfalfa, cauliflower, wheat and cotton were recorded and analyzed with direct current electrical penetration graph (DC-EPG) technique. 【Result】 Observations on five host plants revealed that four waveforms correlated with feeding behavior:Ⅰrepresents stylet thrusting into plant tissue, Ⅱ represents multiple-cell laceration and salivation, Ⅲ represents ingestion, and Ⅳ represents withdrawal of the stylet from the plant tissue. The probe duration and the duration of Ⅲ per probe by A. suturalis on alfalfa were significantly longer than on other hosts, while the insect much preferred the growing point of cotton to other structures (cotyledon and leaf). 【Conclusion】 The probing waveforms of A. suturalis were defined in this experiment. The results by comparing the EPG parameters in the test showed that alfalfa and growing point of cotton in this test were the best feeding plant or structure for the insect.
Helminthiasis of domestic stock in the union of South Africa

In this communication an attempt will be made to present in a concise and convenient form, the more essential points which may interest those who are called upon to deal with outbreaks of helminthiasis amongst domestic animals in South Africa. Details as to the identification, life histories, distribution, curative treatment, prophylaxis, etc., of each individual species to be met with in the various animals are evidently beyond the scope of this paper.
OBSERVATION ON THE KARYOTYPES OF SITOPHILUS ORYZAE AND SITOPHILUS ZEAMAIS AND THEIR HYBRID OFFSPRINGS

Sitophilus oryzae and 5. zeamais are the two snout beetles showing close resemblance and their identification'has caused controversy in the past. We carried out cross experiments for four years and found that the cross-breeding was difficult and the progenies were mostly sterile but about 3% of them were fertile and could produce fertile offsprings. A microscopical examination of the karyotypes revealed that both species contained 22 chromosomes (2n = 22) and-the relative length and centromere pattern of the homologous chromosomes from the first to the tenth pairs and the first pair of nonhornalogous chromosome ate similar. However, the second nonhemologous of; 5. oryzae is metacentric while that of S. zeamais telocentric. There are two karyotypes in the fertile crossbreeds: one is similar to that of S. oryzae and the other-is different from the both species in having the tenth pair of homologous chromosomes being telocentrie.
Redescription of Tetramorium atlante Cagniant, 1970, New Status (Hymenoptera: Formicidae: Myrmicinae)

Abstract. Tetramorium atlante Cagniant, 1970, new status, a member of Tetramorium semilaeve complex is redescribed based on new material from northern Morocco. Detailed descriptions of gyne and male are given for the first time. Diagnosis differentiating this species from T. semilaeve is given, along with colour photographs of all castes and male genitalia.
Toxic Activities of Platycladus orientalis Against Lasioderma serricorne and Tribolium castaneum in Stored Tobacco

In order to effectively control the pests in stored tobacco leaves, new botanical protectants werestudied and developed. The activities of essential oil and isolated components of Platycladus orientalis weretested via fumigation and contact toxicity assays, and the chemical components in the essential oil wereanalyzed by GC/MS. The results showed that:1)A total of 34 components were identified in the essentialoil, which accounted for 93.28% of total components; and the major components were α-pinene(15.08%),cedrol(12.63%), 3-carene(9.68%)and caryophyllene(9.02%). 2)By means of bioactivity screening, theessential oil possessed potential contact toxicity against Lasioderma serricorne(LD50= 50.45 μg/adult)andTribolium castaneum(LD50 = 48.59 μg/adult), and it also possessed fumigation toxicity against Lasioderma serricorne(LC50=145.40 mg/L)and Tribolium castaneum(LC50=39.25 mg/L). 3)Three active components,α-pinene, β-phellandrene and 4-terpinenol, were obtained from the essential oil. It was proved that theessential oil of Platycladus orientalis and its active isolated components possessed certain contact andfumigation toxic activities against Lasioderma serricorne and Tribolium castaneum.
Microfungi Potentially Pathogenic for Humans Reported in Surface Waters Utilized for Recreation

Fungal infections are commonly reported among outdoor bathers. Recreational water reservoirs are an important element of the mycosis epidemiological chain in the biosphere because they can be a source of fungi from municipal wastes, of asymptomatic carriers or bathers carrying mycoses. Yeast-like fungi and moulds were isolated from samples of four surface water baths and five swimming pools in 2011–2012. Membrane filters and standard laboratory-based protocols were used to identify the isolated fungi. In total, 51 saprotrophic and potentially pathogenic species classified in the biosafety level-2 group were found. In one of the eutrophic lakes, a positive correlation was observed between the frequency of trading and the presence of coliform yeasts, while in the second, a correlation was found between the number of species of fungi and the presence of faecal streptococci. In swimming pool waters, the key factors that determined higher numbers of fungi were increased temperature, followed by low concentrations of chlorine ions. Together with the bacteriological and physicochemical analysis, constant mycological monitoring of recreational water (lake pools and swimming pools) should be a standard inspection element to reduce sanitary and epidemiologic risks to people using these water reservoirs for recreational purposes.
Screening and Identification of Amine Oxidase Productive Strains from Smoked Horse Intestine

In order to obtain good amine oxidase productive starters for meat product,amine oxidase productive strains were isolated from 44 samples in different areas of the smoked horse intestine.Productive amine bacteria were eliminated by double color method in monolayer culture.The oxidase test,bacteriostasis experiment and the resistance to salt and nitrite test were conducted for isolated strains.The same strains were eliminated by DGGE and the ability of oxidizing biogenic amine was verified by HPLC.Finally,the DNA was extracted from the screened strains,and V6-V8 segment of 16s rDNA were sequenced after PCR amplification.The results showed that six strains productive amine oxidase bacteria were screened out,with salt tolerance above 6%,nitrite tolerance above 0.15 g/kg,and had good inhibitory effect to Escherichia coli,Listeria monocytogenes,and Staphylococcus aureus,and they had strong oxidizing ability of biogenic amines.The strains were Lactobacillus rhamnosus,Bacillus subtilis,Staphylococcus saprophyticus,Staphylococcus xylosus,Pediococcus pentosaceu and Lactobacillus plantarums.This research provides a good theoretical basis for the industrial production and security of fermented meat.
Salt-Tolerance Rice Mutant Lines Controlled by a Major Effect Gene Were Obtained by Cell Engineering Technique

nthers of rice (Oryza sativa) treated or untreated with ethylmethanesulphonate (EMS) were used to select the NaCl-tolerant calli on N6 agar medium containing 0.5%,0.8% and 1.0% NaCl respectively.Three NaCl-tolerant mutant lines (M1,M2 and M3) were obtained from 22 fertile R1 plants after selection in soil containing 0.5% NaCl.Their salt-tolerant trait have been stably inherited for thirteen generations.In the F2 populations of the hybrids derived from the mutants backcrossed with their parent,one forth plants fell dead in soil containing 0.5% NaCl,three fourths plants seeded normally.This result suggested that the effect of a major gene may be presented in these mutants. 
Pathogen and pathology of "acute death syndrome" of Babylonia areolata

The pathogens of Babylonia areolata " acute death syndrome" in Hainan were isolated and purified,artificially infected,16S rDNA analized,and physiological and biochemical characteristics identificated. The results show that the pathogen is Vibrio harveyi. Drug sensitivity test results show that the pathogen is sensitive to the common antibiosis drugs such as enrofloxacin,sulfa,but resistant to penicillin. Pathological changes in the gill,hepatopancreas,gastropods,digestive tract and kidney are as follows: cell degeneration,necrosis,and blood cell infiltration.
Association of ApoE gene polymorphism with coronary heart disease and lipid metabolism

Objective To investigate and the association of ApoE gene polymorphism with coronary heart disease(CHD). Methods The polymerase chain reaction restriction fragment length polymorphism technology(PCR-RFLP)was used to detect ApoE genotype in 88 CHD patients(according to WHO 1979 criterion) and 75 normal controls.TC、LDL-C、HDL-C、TG、ApoA1、ApoB-100 of fasting serum were measured by anto-biochemical analysis system.Results From our experiment,5 genotypes were found in both CHD and control groups: E2/3,E3/3,E3/4,E2/4 and E4/4.the frequence of E3/3 was highest in both groups.There was a significant difference of genotype distribution between CHD and control group,the frequence of E2/4、E4/4 in CHD group was higher than control,the frequence of E3/3 were lower than control.the frequence of e2 ande4 in CHD group was higher than control,and the frequence of e3 was lower than control.Conclusion Apolipoprotein E gene polymorphism affects serum TC,LDL-C and LP(a) levels in CHD.There was significant association of polymorphism of ApoE gene with CHD,while the distribution of the ApoE genotype was different among three groups.e4 allele is a risk factor in CHD.
In silico prediction of terrestrial and aquatic toxicities for organic chemicals

Qualitative classification and quantitative regression models for fathead minnow and honey bee toxicity prediction were developed using different chemoinformatics techniques such as substructure pattern recognition and different machine learning methods.Specifically,methods include support vector machine,C4.5 decision tree,k-nearest neighbors,random forest and naive bayes.Reliable predictive models were developed and all models were validated by the independent test set.The overall predictive accuracy of the classification models using support vector machine were 95.9% for the fathead minnow test set and 95.0% for the honey bee test set.The square of correlation coefficient of regression models were 0.878 for the fathead minnow test set and 0.663 for the honey bee test set using support vector machine regression algorithm.At last,some representative substructure patterns for characterizing fathead minnow and honey bee toxicity compounds,such as 1,2-diphenol,dialkylthioether,diarylether and phosphoric_ acid_ derivative were also identified via the information gain analysis.The approaches provide a useful strategy and robust tools in the screening of ecotoxicological risk or environmental hazard potential of chemicals.
Isolation and Characterization of Two GDP Dissociation Inhibitor Genes from Oryza sativa L

Using Rho family member OsRacD as bait, two rice GDP dissociation inhibitors genes, designated OsRhoGDI1 and OsRhoGDI2 were isolated by yeast two-hybrid. Filter lift assay and GST pull down assay showed that OsRhoGDI1 and OsRhoGDI2 bound both wild type and constitutive activated OsRacD ,which were independent on the N-terminal part sequence. The binding specificity between OsRhoGDIs and Rho was also confirmed. The two OsRhoGDIs were expressed in shoots, roots and panicle with obviously different pattern. The results confirmed that there was a GDP dissociation inhibitors gene family regulating Rho GTPases states in rice, facilitated further study of Rho pathway.
Study of the relationship between polymorphism of angiotensin converting enzyme gene and vascular dementia

Objective To explore the relationship between polymorphism of angiotensin converting enzyme (ACE) gene and vascular dementia (VD). Methods 94 cases of VD, 60 cases of essential hypertension (EH) and 60 healthy controls were enrolled to analyze insertion/deletion polymorphism of ACE gene by polymerase chain reaction (PCR). Results The DD genotype and the frequency of D allele were significantly higher in VD patients than in healthy controls (all P0.01), and in VD patients with EH than in VD patients without EH (all P0.05). The clinical condition of VD was not associated with the polymorphism of ACE gene.Conclusions There is a relationship between polymorphism of ACE gene and VD. DD genotype and D allele may be risk factors of VD.
Scale dependence in desert plant biodiversity

The relationship between the number of species and the area sampled is one of the oldest and best-documented patterns in community ecology. Species-area relationships have been widely used to examine the latitudinal gradient in species diversity, the effect of habitat loss on species diversity and the impact of exotic species on species diversity. An equation of the form S=cA~z describing more precisely the species-area relation for plant species in smaller area is proposed as a result of intensive examination of species presence. Several studies and field data from a wild range of plant and animal taxa suggest that the slope, z, of a graph of the logarithm of species richness against the logarithm of area is not a constant to the grassland and forest communities. This study was conducted to examine which the characteristic of species diversity of desert community and the change laws of z with spatial scales variance are appropriate for inference which z is not a constant, and the other purpose of this paper was to reveal the distinction of species diversity between desert community and grassland or forest community.We collected replicated and randomized plant data at 6 spatial scales from 1 m~2, 10 m~2, 100 m~2, 1000 m~2, 10000 m~2 to 1 km~2 in the desert region of west China. The results showed that the slope of the log-log plot varied systematically with spatial scale, the value of z was high (0.37) at small scales from 1m~2 to 10 m~2, but it decreased with spatial scales increasing. When spatial scales varied from 1m~2 to 1 km~2, the value of z varied from 0.37 to 0.035. Furthermore, when the replication at each scale is used (in a log-linear model corrected for over dispersion), it was found that highly significant differences in slope from scale to scale, for example, at small scales from 1m~2 to 100 m~2, regressions of log (species richness) against log (area) gave slope of 0.273 (SE=0.087), but at larger scales from 100 m~2 to 1 km~2, the value of slope was 0.0396 (SE=0.0039). These results described that species richness of desert community at small scale (less than 100m~2) had strongly scale dependence, and were different from the research of grassland and forest communities. They directed attention to the need to develop a more mechanistic understanding of patch patterns and coexistence at small scales in the desert community.
The influence of the DnaA protein on transcription of the ftsZ and DnaA genes in Escherichia coli

The investigation of the mechanisms governing the control of chromosomal DNA synthesis and cell division is fundamental to the understanding of the regulation of the cell cycle in Escherichia coli. Research over many years has shown that two proteins are central to these processes: DnaA in initiation of chromosomal replication and FtsZ in cell division. DnaA and FtsZ are not only thought to be essential to the biochemistry of these events but appear also to be involved in their timing within the cell-cycle. For this reason expression of both the dnaA and ftsZ genes are regulated by a number of different mechanisms, presumably to ensure efficient growth and division of the organism under a wide range of environmental conditions. In addition to its aforementioned role, DnaA also functions as a regulatory protein in the expression of a number of genes. Its effect is mediated by a particular recognition sequence present at its site of action, known as the DnaA-box. Such a sequence is present in the promoter region of the dnaA gene and has in the past been reported to be involved in autoregulation of this gene. In this work this putative role of the DnaA protein is reassessed and cast in doubt. The promoter region of the ftsZ gene also contains a number of DnaA boxes and some evidence exists that DnaA may be involved inftsZ regulation. In this work evidence is presented to the contrary and it is shown that the apparent role of DnaA inftsZ regulation is probably due to regulation of the gene by a growth-rate sensitive mechanism.
Reconstruction with free flaps in robotic head-and-neck onco-surgeries

ABSTRACT Aims and Objective: The aim of the present article is to highlight how reconstruction with free flaps is different and difficult in cases with robotic head-and-neck cancer surgery. It also highlights the technical guidelines on how to manage the difficulties. Materials and Methods: Eleven patients with oropharyngeal cancer having undergone tumour excision followed by free-flap reconstruction been reviewed here. Nine patients had tumour excision done robotically through intraoral route while neck dissection done with transverse neck crease incision. There is a problem of difficult flap inset in this group of patient. Two patients had intraoral excision of tumour followed by robotic neck dissection via retroauricular incision. With no incision directly on the neck, microvascular anastomosis is challenging in this set of patients. Free flap was used in all the cases to reconstruct the defect. Results: Successful reconstruction with free flap was done in all the cases with good outcome both functionally and aesthetically. Conclusion: Free-flap reconstruction is possible in robotic head-and-neck cancer surgery despite small and difficult access, but it does need practice and some technical modifications for good outcome.
Immobilization of β-glucosidase by sodium alginate

β-Glucosidase was immobilized by using sodium alginate as carrier.The immobilized methods and conditions were studid.The immobilized enzyme was used to hydrolyze cellobiose and cellulose farther.The results show that the best efficiency of immobilization was obtained by using the crosslinking-embedding method when sodium alginate concentration was 3.5%,β-glucosidase dosage was 100 U/g carrier,glutaraldehyde concentration was 1%,CaCl2 concentration was 2%,and immobilized time was 2 h.In repeated batch process of hydrolysis cellobiose,the yield reached enzymatic hydrolysis kept highter than 90% during 20 batches.In continuous process of hydrolysis cellobiose,the yield of enzymatic hydrolysis changed with the feed-in rate.The yield reached 96.7% and 99.0%,when the rate was 1.5 mL/min,1.0 mL/min,respectively.The synergetic hydrolysis processes on filter paper and Avicel were carried out by the cellulose of T.viride and the immobilized β-glucosidase.Under the condition of total β-glucosidase activity to filter paper activity was increased to 0.5 and FPA was 2.0 U/mL at 60 h,the degradation rate of filter paper and Avicel was increased by 20.4% and 29.3%,respectively as compared to that using T.viride only.This research provided a more efficient way to improve the hydrolysis yield and lower cost of enzyme for cellulosic material hydrolysis.
Isolation,Identification and Induced Differentiation of Bovine Skeletal Muscle Satellite Cells

In this study,bovine skeletal muscle satellite cells were isolated and identified,muscle tissues were digested by collagenase and trypsinase,then the satellite cells were purified and induced differentiate into myotubes.The muscle satellite cells and myotubes were observed,and detected by RT-PCR and immunofluorescent staining technology.The results showed that the muscle satellite cells were in healthy morphology,and more than 93 percent of the cells expressed the marker genes paired box protein 7(Pax7)and myogenic differentiation antigen(MyoD). After myogenesis induction,satellite cells began to convergence,and finally formed multinucleated myotubes expression of myogenin(MyoG)and myosin heavy chain(MHC). Our results indicated that high purity skeletal muscle satellite cells were successfully isolated and identification,which could provide cell source for muscle development and bovine muscle quality improvement study.
Quantifying the psychological properties of words

This thesis explores the psychological properties of words – the idea that words carry links to additional information beyond their dictionary meaning. It does so by presenting three distinct publications and an applied project, the Macroscope. The published research respectively covers: the modelling of language networks to explain lexical growth; the use of high dimensional vector representations of words to discuss language learning; and the collection of a normative dataset of single word humour ratings. The first publication outlines the use of network science in psycholinguistics. The methodology is discussed, providing clear guidelines on the application of networks when answering psychologically motivated questions. A selection of psychological studies is presented as a demonstration of use cases for networks in cognitive psychology. The second publication uses referent feature norms to represent words in a high dimensional vector space. A correlative link between referent distinctiveness and age of acquisition is proposed. The shape bias literature (the idea that children only pay attention to the shape of objects early on) is evaluated in relation to the findings. The third publication collects and shares a normative dataset of single word humour ratings. Descriptive properties of the dataset are outlined and the potential future use in the field of humour is discussed. Finally, the thesis presents the Macroscope, a collaborative project put together with Li Ying. The Macroscope is an online platform, allowing for easy analysis of the psychological properties of target words. The platform is showcased, and its full functionality is presented, including visualisation examples. Overall, the thesis aims to give researchers all that’s necessary to start working with psychological properties of words – the understanding of network science in psycholinguistics, high dimensional vector spaces, normative datasets and the applied use of all the above through the Macroscope.