Miracle-dz/UniFAR-FTUs huggingface dataset:
Biomarkers of disease in human nails: a comprehensive review

Abstract Diagnostic, monitoring, response, predictive, risk, and prognostic biomarkers of disease are all widely studied, for the most part in biological fluids or tissues, but there is steadily growing interest in alternative matrices such as nails. Here we comprehensively review studies dealing with molecular or elemental biomarkers of disease, as opposed to semiological, pharmacological, toxicological, or biomonitoring studies. Nails have a long history of use in medicine as indicators of pathological processes and have also been used extensively as a matrix for monitoring exposure to environmental pollution. Nail clippings are simple to collect noninvasively as well as to transport and store, and the matrix itself is relatively stable. Nails incorporate, and are influenced by, circulating molecules and elements over their several months of growth, and it is widely held that markers of biological processes will remain in the nail, even when their levels in blood have declined. Nails thus offer the possibility to not only look back into a subject’s metabolic history but also to study biomarkers of processes that operate over a longer time scale such as the post-translational modification of proteins. Reports on ungual biomarkers of metabolic and endocrine diseases, cancer, and psychological and neurological disorders will be presented, and an overview of the sampling and analytical techniques provided.
Prevalence of teen dating violence in Europe: A systematic review of studies since 2010.

Violence in adolescent relationships is a common problem with numerous negative short- and long-term consequences. Because most of the evidence on teen dating violence (TDV) synthesized in reviews comes from North American studies, this review aimed to compile evidence on prevalence rates of TDV based on studies identified for Europe only. Specifically, we considered different forms of TDV victimization and perpetration, gender differences, and its measurement. A systematic literature search of the most popular databases Ebsco and PubMed yielded a total of N = 34 studies, with most of the studies identified for Spain, and only a few studies in other European countries. In sum, the results revealed a great variability in prevalence rates across and within the European countries, a common pattern of gender differences, and a wide range of applied measurements, corresponding with the evidence from the North American studies. Implications for future research and policy were discussed.
Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans

To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell autonomous. We have discovered that, in Caenorhabditis elegans, neuronal heat shock factor 1 (HSF-1), the conserved master regulator of the heat shock response (HSR), causes extensive fat remodeling in peripheral tissues. These changes include a decrease in fat desaturase and acid lipase expression in the intestine and a global shift in the saturation levels of plasma membrane’s phospholipids. The observed remodeling of plasma membrane is in line with ectothermic adaptive responses and gives worms a cumulative advantage to warm temperatures. We have determined that at least 6 TAX-2/TAX-4 cyclic guanosine monophosphate (cGMP) gated channel expressing sensory neurons, and transforming growth factor ß (TGF-β)/bone morphogenetic protein (BMP) are required for signaling across tissues to modulate fat desaturation. We also find neuronal hsf-1 is not only sufficient but also partially necessary to control the fat remodeling response and for survival at warm temperatures. This is the first study to show that a thermostat-based mechanism can cell nonautonomously coordinate membrane saturation and composition across tissues in a multicellular animal.
Distributed Fault-Tolerant Containment Control Protocols for the Discrete-Time Multiagent Systems via Reinforcement Learning Method

This article investigates the model-free fault-tolerant containment control problem for multiagent systems (MASs) with time-varying actuator faults. Depending on the relative state information of neighbors, a distributed containment control method based on reinforcement learning (RL) is adopted to achieve containment control objective without prior knowledge on the system dynamics. First, based on the information of agent itself and its neighbors, a containment error system is established. Then, the optimal containment control problem is transformed into an optimal regulation problem for the containment error system. Furthermore, the RL-based policy iteration method is employed to deal with the corresponding optimal regulation problem, and the nominal controller is proposed for the original fault-free system. Based on the nominal controller, a fault-tolerant controller is further developed to compensate for the influence of actuator faults on MAS. Meanwhile, the uniform boundedness of the containment errors can be guaranteed by using the presented control scheme. Finally, numerical simulations are given to show the effectiveness and advantages of the proposed method.
Metformin induces muscle atrophy by transcriptional regulation of myostatin via HDAC6 and FoxO3a

Skeletal muscle atrophy is a severe condition that involves loss of muscle mass and quality. Drug intake can also cause muscle atrophy. Biguanide metformin is the first‐line and most widely prescribed anti‐diabetic drug for patients with type 2 diabetes. The molecular mechanism of metformin in muscle is unclear.
Hypothermic oxygenated machine perfusion for extended criteria donor allografts - preliminary experience with extended organ preservation times in the setting of organ reallocation.

BACKGROUND In times of critical organ shortage, poor organ pool utilization and increased use of extended-criteria donor (ECD) allografts remain a major problem. Hypothermic oxygenated machine perfusion (HOPE) has emerged as a promising and feasible strategy in ECD liver transplantation. However, potential safety limits regarding the duration of perfusion are yet to be explored. Besides marginal allograft quality (steatosis), prolonged cold ischemia time remains the most important factor for a high number of liver allografts being declined for transplantation. PATIENTS & METHODS Two ECD-allografts were each allocated to two recipients, who proved to be unsuitable to receive the assigned allograft upon arrival at the transplant center. The organs were reallocated by Eurotransplant and accepted by our center for two different backup patients. During that time, HOPE was commenced and continued until recipient hepatectomy was completed. Postoperative allograft function was assessed by serum levels of ALT, AST, bilirubin and INR. Incidence of early allograft dysfunction (EAD), postoperative complications and length of hospital stay were analyzed. RESULTS HOPE was applied for 4h35min and 4h20min, resulting in total cold preservation time of 17h29min and 15h20min, respectively. Both recipients displayed decreasing serum transaminases and bilirubin levels postoperatively. No EAD or major postoperative complications occurred in either patient. Serum ALT and AST levels were within normal range at discharge. CONCLUSIONS Extended HOPE enables safe extension of preservation time for up to 18 hours in human liver transplantation. End-ischemic HOPE may significantly improve organ pool utilization, while simultaneously facilitating operating room logistics and preventing organ injury.
High-pressure phase transition of bismuth

ABSTRACT The high-pressure phase transition of bismuth, Bi, has been investigated using an in-situ X-ray powder diffraction measurement at pressures and temperatures up to 8.1 GPa and 440 K, respectively. The pressure-induced phase transition from Bi-III to Bi-V was observed. This transition occurred at 7.6 GPa and at room temperature, where a negative temperature dependence for this transition was confirmed. The transition boundary was determined to be .
Direct Synthesis of Graphene Quantum Dots with Different Fluorescence Properties by Oxidation of Graphene Oxide Using Nitric Acid

Graphene quantum dots (GQDs) play a critical role in many applications in the electrical and optical fields. We develop a simple three-step hydrothermal etching method to prepare GQDs by adopting graphene oxide (GO) as a precursor and nitric acid as an oxidant. We discuss the formation mechanism of GQDs by the characterization of products and intermediates with Scanning electronic microscopy (SEM), Transmission electron microscopic (TEM), Raman, Fourier transform infrared (FT-IR) spectroscopy and X-ray photoelectron spectroscopy (XPS). Two kinds of GQDs have been obtained after the treatment of GO with different concentrations of nitric acid. The sizes of GQDs are small, with diameters of 3.38 nm and 2.03 nm on average, respectively. When excited with 365 nm UV light, the two kinds of GQDs exhibit green and yellow luminescence; the different optical properties can be attributed to the differences in degree of oxidation and nitrogen doping. The result is important for GQDs in synthesizing and optical field.
Cassava (Manihot esculenta Crantz) stump biochar: Physical/chemical characteristics and dye affinity

Abstract Aiming investigated the pyrolysis of the agricultural waste known as cassava stump, the portion of the plant to which the tuberous roots and aerial parts of the plant are attached, this study had as objective to produce biochars from cassava stump by conducting vacuum pyrolysis at 400, 500, or 600 °C. The biochars were characterized by proximate analysis, thermogravimetric analysis, Fourier-transform infrared absorption spectroscopy, X-ray diffraction, scanning electron microscopy, and nitrogen adsorption. Adsorption affinity tests were performed with four different dyes: methylene blue, basic fuchsin, acid fuchsin, and alizarin. Biochar obtained at 500 °C, heating rate of 20 °C.min−1, and 90 min of residence at the final temperature, had 22% higher fixed carbon content as compared to the other biochars and 3.16 times greater fixed carbon content than the original cassava stump. This biochar showed the best adsorption capacity (0.0679 mmol/g) and percentage of removal (87.6%) of methylene blue dye from aqueous solution. The material characterization reveals that biochars from Manihot esculenta Crantz stump may have potential application in carbon sequestering. Besides that, these biochars could be applied with efficiency as adsorbent of dyes.
Histo-Blood Group Antigens, Enteropathogen Carriage and Environmental Enteropathy in Stunted Zambian Children

ABSTRACT Objectives: Stunting, the most common form of childhood undernutrition, is associated with environmental enteropathy (EE). Enteric infections are believed to play a role in the pathophysiology of EE and stunting though the exact mechanism remains undetermined. The FUT2 (secretor) and FUT3 (Lewis) genes have been shown to be associated with some symptomatic enteric infections in both children and adults. These genes are responsible for the presence of histo-blood group antigens (HBGAs) in various secretions and epithelial surfaces. We evaluated whether the secretor and Lewis status influences asymptomatic enteric infections and thus EE severity on duodenal biopsies of stunted children. Methods: In this case-control study, we used saliva samples to determine the secretor and Lewis status of stunted children (cases, n = 113) enrolled in a nutritional rehabilitation program and from their well-nourished counterparts (controls, n = 42). Where available, saliva was also collected from the mothers. Baseline stool samples were used to detect asymptomatic enteropathogen carriage. Duodenal biopsies were collected from a subgroup of stunted children (n = 77) who had an upper gastrointestinal endoscopy done as part of the evaluation process for their non-response to nutritional therapy. Results: The proportion of secretors was similar between the cases and the controls (82% vs 81%, P = 0.81). The stunted children had significantly higher rates of carrying multiple enteropathogens, but this was not associated with their secretor status nor that of their mothers. The secretor status was also not associated with mucosal morphometry of duodenal biopsies. Conclusion: This case-control analysis in Zambian children does not support the hypothesis that fucosylation status determines asymptomatic enteropathogen carriage in stunting.
A venom protein of ectoparasitoid Pachycrepoideus vindemiae, PvG6PDH, contributes to parasitism by inhibiting host glucose‐6‐phosphate metabolism

To achieve successful development, female parasitoids, while laying eggs, introduce various virulence factors, mainly venoms, into host insects to manipulate their physiology. Although numerous studies have been conducted to characterize the components of venoms that regulate host immune responses, few systematic investigations have been conducted on the roles of venom proteins in host metabolic regulation. In this investigation, we characterized a novel venom protein in Pachycrepoideus vindemiae called glucose‐6‐phosphate dehydrogenase (PvG6PDH) and showed it has a vital role in regulating host carbohydrate metabolism. PvG6PDH encodes 510 amino acids and features a signal peptide and two conserved “G6PDH” domains. Multiple sequence alignment showed it has high amino acid identity with G6PDH from other pteromalids, and quantitative polymerase chain reaction analysis and immunofluorescent staining demonstrated a significantly higher expression of PvG6PDH in the venom apparatus compared with the carcass. We report that PvG6PDH contributes to parasitism by inhibiting the glucose‐6‐phosphate (G6P) metabolism of host Drosophila melanogaster, as demonstrated by PvG6PDH injection and RNA interference analysis. Further tests revealed that the accumulation of host G6P was caused by the transcriptional inhibition of G6P‐metabolism‐related genes. These findings greatly contribute to our understanding of venom‐mediated host metabolic regulation, further laying the foundation for the development of venom proteins as biological agents for pest control.
Rv0180c contributes to Mycobacterium tuberculosis cell shape and to infectivity in mice and macrophages

Mycobacterium tuberculosis, the main causative agent of human tuberculosis, is transmitted from person to person via small droplets containing very few bacteria. Optimizing the chance to seed in the lungs is therefore a major adaptation to favor survival and dissemination in the human population. Here we used TnSeq to identify genes important for the early events leading to bacterial seeding in the lungs. Beside several genes encoding known virulence factors, we found three new candidates not previously described: rv0180c, rv1779c and rv1592c. We focused on the gene, rv0180c, of unknown function. First, we found that deletion of rv0180c in M. tuberculosis substantially reduced the initiation of infection in the lungs of mice. Next, we established that Rv0180c enhances entry into macrophages through the use of complement-receptor 3 (CR3), a major phagocytic receptor for M. tuberculosis. Silencing CR3 or blocking the CR3 lectin site abolished the difference in entry between the wild-type parental strain and the Δrv0180c::km mutant. However, we detected no difference in the production of both CR3-known carbohydrate ligands (glucan, arabinomannan, mannan), CR3-modulating lipids (phthiocerol dimycocerosate), or proteins in the capsule of the Δrv0180c::km mutant in comparison to the wild-type or complemented strains. By contrast, we established that Rv0180c contributes to the functionality of the bacterial cell envelope regarding resistance to toxic molecule attack and cell shape. This alteration of bacterial shape could impair the engagement of membrane receptors that M. tuberculosis uses to invade host cells, and open a new perspective on the modulation of bacterial infectivity.
Quantifying the Acute Care Costs of Neonatal Bacterial Sepsis and Meningitis in Mozambique and South Africa

Abstract Background Sepsis and meningitis are among the leading causes of neonatal deaths in sub-Saharan Africa (SSA). Neonatal sepsis caused ~400 000 deaths globally in 2015, half occurring in Africa. Despite this, there are few published data on the acute costs of neonatal sepsis or meningitis, with none in SSA. Methods We enrolled neonates admitted to 2 hospitals in South Africa and Mozambique between 16 April 2020 and 1 April 2021. In South Africa all cases were microbiologically confirmed, but in Mozambique both clinically suspected and microbiologically confirmed cases were included. Data were collected on healthcare resource use and length of stay, along with information on household expenditure and caregiving. We used unit costs of healthcare resources in local currencies to estimate healthcare provider costs per patient and costs per household. Results were converted to 2019 international dollars (I$). Results We enrolled 11 neonates in Mozambique and 18 neonates in South Africa. Mean length of stay was 10 days (median, 9 [interquartile range {IQR}, 4–14) and 16 days (median, 15 [IQR, 13–18]), respectively. In Mozambique we estimated mean household costs of I$49.62 (median, 10.19 [IQR, 5.10–95.12]) and hospitalization costs of I$307.58 (median, 275.12 [IQR, 149.43–386.12]). In South Africa these costs were I$52.31 (median, 30.82 [IQR, 19.25–73.08]) and I$684.06 (median, 653.62 [IQR, 543.33–827.53]), respectively. Conclusions We found substantial costs associated with acute neonatal bacterial (all-cause) sepsis and meningitis in SSA. Our estimates will inform economic evaluations of interventions to prevent neonatal invasive bacterial infections.
Global variation in soil carbon sequestration potential through improved cropland management

Widespread adoption of improved cropland management measures is advocated to increase soil organic carbon (SOC) levels, thereby improving soil fertility and mitigating climate change. However, spatially explicit insight on management impacts is limited, which is crucial for region‐specific and climate‐smart practices. To overcome these limitations, we combined global meta‐analytical results on improved management practices on SOC sequestration with spatially explicit data on current management practices and potential areas for the adoption of these measures. We included (a) fertilization practices, i.e., use of organic fertilizer compared to inorganic fertilizer or no fertilizer, (b) soil tillage practices, i.e., no‐tillage relative to high or intermediate intensity tillage, and (c) crop management practices, i.e., use of cover crops and enhanced crop residue incorporation. We show that the estimated global C sequestration potential varies between 0.44 and 0.68 Gt C yr−1, assuming maximum complementarity among all measures taken. A more realistic estimate, not assuming maximum complementarity, is from 0.28 to 0.43 Gt C yr−1, being on the lower end of the current range of 0.1–2 Gt C yr−1 found in the literature. One reason for the lower estimate is the limited availability of manure that has not yet been recycled. Another reason is the limited area for the adoption of improved measures, considering their current application and application limitations. We found large regional differences in carbon sequestration potential due to differences in yield gaps, SOC levels, and current practices applied. The highest potential is found in regions with low crop production, low initial SOC levels, and in regions where livestock manure and crop residues are only partially recycled. Supporting previous findings, we highlight that to encourage both soil fertility and SOC sequestration, it is best to focus on agricultural soils with large yield gaps and/or where SOC values are below levels that may limit crop production.
Effect of Cadherin-11 Expression on the Prognosis of a Newly Diagnosed Primary Glioblastoma

Background Cadherin-11, a cell-to-cell adhesion molecule, is associated with higher tumor grade and decreased patient survival. The purpose of this study was to investigate the clinical significance of cadherin-11 expression in the progression and prognosis of a newly diagnosed primary glioblastoma (GBL). Methods Between 2007 and 2016, 52 out of 178 patients diagnosed with a GBL and satisfied the following criteria: 1) a new primary GBL, 2) gross-total resection, 3) immunohistochemically-available tissue, and 4) standardized adjuvant treatment. Results In terms of staining intensity, the low-intensity cadherin-11 group showed longer progression-free survival (PFS) than the high-intensity cadherin-11 group (median PFS, 12.0 months [95% CI, 11.1–12.9] vs. median PFS, 6.0 months [95% CI, 3.7–8.3]; p<0.001). The low-intensity cadherin-11 group revealed longer overall survival (OS) than the high-intensity cadherin-11 group (median OS, 20.0 months [95% CI, 11.8–16.6] vs. median OS, 15.0 months [95% CI, 11.8–18.2]; p=0.003). The staining intensity of cadherin-11 was a statistically significant factor in PFS and OS in terms of univariate and multivariate analyses (univariate analysis: p<0.001 and p=0.005; multivariate analysis: p<0.001 and p=0.005). Conclusion Our clinical study demonstrates high cadherin-11 expression may be associated with poor PFS and OS for a newly diagnosed primary GBL.
Field-frequency lock approach for 21.3-MHz high-performance NMR relaxation analyzer

Low-field NMR (LF-NMR) relaxation analyzers have been more and more widely used in food science, biomedicine, and petroleum exploration in recent years. An LF-NMR device analyzes various interactions between sample molecules through spin relaxation, diffusion, flow, and imaging experiments. However, temporal field fluctuations in the magnet limit the scope of application. A stable magnetic field is the basic guarantee for detecting weak signals with shorter relaxation time. This paper presents an approach involving a field-frequency lock for a 21.3-MHz high-performance NMR bipolar permanent magnet. The diameter spherical volume (DSV) of the magnet is only 60 mm. The field-frequency lock system uses 19F in a micro coil integrated into the main probe as a lock detector. The required magnetic field compensation can be calculated from the lock free induction decay (FID) signal frequency, which is measured by lock spectroscopy. The compensation coils used to produce the magnetic field were designed based on Helmholtz coils. The system determines the signal frequency by detecting the lock FID signal and calculates the required compensation-coil current to stabilize the main magnetic field of the analyzer. The results of practical locking experiments in a 21.3-MHz high-performance NMR relaxation analyzer showed that this new approach helps to reduce magnetic field fluctuations from 11 ppm/3h (11 × 10–6) to 0.4 ppm/3h (0.4 × 10–6), which meets the application requirements. This approach is especially viable and effective for a permanent magnet with large field fluctuations. This paper also provides observations of the effect of these fluctuations on NMR measurements before and after installation of the field-frequency lock system.