Miracle-dz/UniFAR-FTUs huggingface dataset:
Development of PBL in the Training of Clinical Psychologists

Problem Based Learning (PBL) is a recent introduction into the training of clinical psychologists and seems to be a promising strategy for supporting the development of competencies in trainees on doctoral programmes. This paper reports on the introduction and assessment of PBL in a hybrid curriculum and relates this to competence and learning outcomes. Issues in evaluation are considered. Early indications suggest that both trainees and programme staff recognise the value of PBL in acquiring skills required in clinical practice. PBL is being developed in a variety of ways that will provide more information on the transferability and generalisability of this approach.
Molecular cloning and expression during spermatogenesis of a cDNA encoding testicular 11beta-hydroxylase (P45011beta) in rainbow trout (Oncorhynchus mykiss).

Cytochrome P450 1beta-hydroxylase (P450(11beta)) is an important steroidogenic enzyme for glucocorticoid and mineralocorticoid production in vertebrates. In teleosts, P450(11beta) also plays a role in the production of 11-ketotestosterone (11-KT), the predominant androgen in male fish. In this study, we cloned a cDNA encoding rainbow trout (Oncorhynchus mykiss) testicular P450(11beta). The cDNA contains 1,740 nucleotides that encode a protein of 551 amino acids which shares 65.2% homology with testicular P450(11beta) from Japanese eel, and 33-45% homology with adrenal P450(11beta) from rat, human, and frog. HEK293 cells transfected with an expression vector containing the rainbow trout P450(11beta) cDNA open reading frame showed 11beta-hydroxylating activity in the presence of exogenous testosterone. Analysis of tissue distribution by RT-PCR showed great abundance of P450(11beta) mRNA in testis and head kidney. In order to clarify the sites of P450(11beta) gene expression, cRNA in situ hybridization analysis was performed. Hybridization signals were detected in Leydig cells and head kidney inter-renal cells. The results of Northern blot analysis indicated a single 1.8-kb transcript encoding P450(11beta) in testis and in head kidney, suggesting that the testicular form of P450(11beta) may also be involved in cortisol production by inter-renal cells. Seasonal changes in total P450(11beta) mRNA levels in testes during spermatogenesis showed a pattern similar to that of plasma androgens. These results suggest that androgen production in male rainbow trout is partially regulated by changes in abundance of P450(11beta) mRNA.
Thymidine diphosphate 4-acetamido-2,6-dideoxyhexoses. 3. Purification and properties of thymidine diphosphate 4-keto-6-deoxy-D-glucose transaminase from Escherichia coli strain B.

Abstract A pyridoxal phosphate-requiring transaminase which catalyzes a reversible reaction between thymidine diphosphate 4-keto-6-deoxy-d-glucose and l-glutamate to form TDP-4-amino-4,6-dideoxy-d-glucose and α-ketoglutarate has been purified 28-fold from an extract of Escherichia coli strain B. Some properties of the enzyme are reported. Transaminase activity is widely distributed among strains of E. coli, Salmonella, and Pasteurella pseudotuberculosis, but among the organisms studied only E. coli strain B has a transaminase which catalyzes the synthesis of TDP-4-amino-4,6-dideoxy-d-glucose. The other organisms have an enzyme which catalyzes the synthesis of the nucleotide containing the sugar with the d-galacto configuration.
Detection of Bioelectromagnetic Signals Transmitted Through the Exoskeleton of Living Land Snails

Background: Our previous reports have used a novel method for imaging bioelectromagnetic energy as electromagnetic fields (EMFs) emanating from plant and animal tissue The purpose of this study is to demonstrate that living land snails exhibit EMFs that can be transmitted through their exoskeleton and can be detected utilizing a mixture of fine iron particles and Prussian Blue Stain (PBS Fe2). Methods: Living intact land snails, 15 to 20 mm in length, were placed on glass slides, two drops of a PBS Fe2 solution were delivered under the snail’s shell. After total evaporation the snails were removed and microphotographs of areas under the snails were viewed via a video microscope. Results: EMFs were imaged as specific patterns of iron aggregates directly under the contact area of the shell and evaporated solution; whereas such iron aggregate patterns were not observed in the presence of empty shells. Conclusions: Living gastropods, such as small snails, emit bioelectromagnetic energy in the form of EMFs transmitted through their exoskeletons to be imaged as specific patterns of iron aggregation subsequent to evaporation. No similar patterns were seen when empty shells were substituted for the live snails.
Trader Identity , Trade Size and Adverse Selection Costs

The purpose of the current research is to empirically establish the relationship between trade size and adverse selection cost and, in so doing, shed further light on the stealth trading literature of Barclay and Warner (JFE, 1993) and Chakravarty (JFE, 2001) who report a significant cumulative price impact of relatively smaller (medium size) trades. In contrast, we find that larger trades are more likely to have higher adverse selection costs than smaller size trades. We further find that institutional trades are associated with higher adverse selection costs overall than individual trades. These results appear to support an important intuition formalized in theoretical market microstructure models.
Walking training affects dehydroepiandrosterone sulfate and inflammation independent of changes in spontaneous physical activity

ObjectiveWe hypothesized that physical exercise in postmenopausal women could interfere with the molecular interrelationship of the immune-endocrine system and be effective even in women in whom training determined a reduction of spontaneous physical activity (SPA). For this reason, we investigated the effects of an aerobic program on plasma dehydroepiandrosterone sulfate (DHEA-S) and cytokine levels in relationship to SPA modification. MethodsThirty-two postmenopausal women (mean [SD] age, 56.38 [4.33] y) were enrolled in the study. Inclusion criteria were as follows: age younger than 65 years, body mass index higher than 18.5 and lower than 35 kg/m2, no pharmacological treatments, and no history of chronic, cardiovascular, or orthopedic diseases. Before and after 3 months of walking training at moderate intensity (40-50 min, 4 d/wk), they were evaluated for SPA, body composition, energy intake, and levels of plasma cytokines (tumor necrosis factor &agr; [TNF-&agr;], interleukin [IL]-1&agr;, IL-1&bgr;, IL-2, IL-8, and IL-10), C-reactive protein, DHEA-S, cortisol, and estrogen. ResultsAt baseline, SPA did not correlate with either DHEA-S level or cytokine levels. There was negative correlation between DHEA-S and both TNF-&agr; and IL-2. After the intervention program, 16 women showed increased SPA, and 16 women showed decreased SPA. Independent of these changes in SPA, both TNF-&agr; levels and cortisol-to-DHEA-S ratio decreased, whereas DHEA-S levels increased. ConclusionsIn postmenopausal women, walking training, rather than SPA, influences DHEA-S and cytokine concentrations and their correlations, thus interfering with adrenal steroids and the inflammatory markers network. Physical exercise acts in parallel on menopausal neuroendocrine alterations and on the systemic inflammatory profile independent of SPA changes.
Essential Roles of IL-6 Trans-Signaling in Colonic Epithelial Cells, Induced by the IL-6/Soluble–IL-6 Receptor Derived from Lamina Propria Macrophages, on the Development of Colitis-Associated Premalignant Cancer in a Murine Model

Activation of the IL-6/Stat3 via IL-6 trans-signaling plays an important role in the pathogenesis of inflammatory bowel disease. Colitis-associated cancer (CAC) is a large bowel cancer and occurs with long-standing inflammatory bowel disease. The role of the IL-6/Stat3 in the development of CAC has not been fully understood. We investigate whether IL-6 trans-signaling contributes to the development of CAC using a mouse colitis-associated premalignant cancer (CApC) model. Chronic colitis (CC) was induced in BALB/c mice using dextran sodium sulfate. CApC was induced by dextran sodium sulfate treatment to CC-affected mice. IL-6 expression was determined by quantitative RT-PCR and immunofluorescence staining in colon. Phospho-Stat3 expression was examined by Western blotting and immunofluorescence analysis. The expression of IL-6 receptors (i.e., the IL-6R α-chain and gp130) and tumor necrosis factor-α converting enzyme in the colon was examined by laser-capture microdissection and immunofluorescence staining. Soluble IL-6Rα (sIL-6Rα) was examined by Western blotting of epithelial cell-depleted colonic tissues. We also investigated whether a soluble gp130-Fc fusion protein could prevent CApC. IL-6 expression was increased in the colon of CC- and CApC-affected mice and was restricted to lamina propria-macrophages. The expression of IL-6Rα and tumor necrosis factor-α converting enzyme was increased in the lamina propria CD11b-macrophages of CC-affected mice. sIL-6Rα expression was also increased in these tissues. Reduced levels of IL-6Rα generation were observed in the colonic epithelial cells of CC- and CApC-affected mice and were associated with the increased expression of gp130 and phospho-Stat3. Treatment with soluble gp130Fc significantly reduced the CApC. IL-6 trans-signaling in epithelial cells induced by macrophage-derived IL-6/sIL-6Rα plays a crucial role in the development of CAC.
Patterns of lymph node metastasis in carcinoma of the distal bile duct.

BACKGROUND/AIMS The aim of this study was to clarify the lymphatic spread in distal bile duct carcinoma in relation to local tumor extent (pT category). METHODOLOGY The distribution and number of involved nodes were examined in 42 patients with distal bile duct cancer. RESULTS Lymph node metastases were present in 25 of 42 patients (60%); 5 of 12 (42%) with pT2 tumors and 20 of 26 (77%) with pT3 tumors. Nodal involvement was not identified in 4 patients with pT1 tumors. The incidence of node positivity was higher in patients with pT3 tumors than in those with pT1 or pT2 tumors (p<0.01 or p<0.05). The total number of positive nodes was greater in patients with pT3 tumors than in those with pT2 tumors (mean: 3.08 vs. 0.75; p<0.01). All node-positive patients had involved nodes in the hepatoduodenal ligament or posterior pancreaticoduodenal region, or both. The number of positive nodes in the superior mesenteric and para-aortic region was greater in patients with pT3 tumors than in those with pT2 tumors (p<0.05). CONCLUSIONS As tumor invades the pancreas from the distal bile duct, lymphatic spread extends from the hepatoduodenal ligament or posterior pancreaticoduodenal region to the superior mesenteric and para-aortic nodes.
Estimating effects of permanent environment, lactation stage, age, and pregnancy on test-day yield.

Test-day variances for permanent environmental effects within and across parities were estimated along with lactation stage, age, and pregnancy effects for use with a test-day model. Data were test-day records for calvings since 1990 for Jerseys and for Holsteins from California, Pennsylvania, Texas, and Wisconsin. Single-trait repeatability models were fitted for milk, fat, and protein test-day yields. Method R and a preconditioned conjugate gradient equation solver were used for variance component estimation because of large data sets. Test-day yields were adjusted for environmental effects of calving age, calving season, and milking frequency and for estimated breeding value (EBV) expressed on a daily basis. To assess the effect of adjustments, test-day yields also were analyzed without adjustment. For adjusted data, permanent environmental variances across parities relative to phenotypic variance ranged from 8.3 to 15.2% for milk, 4.4 to 8.3% for fat, and 6.9 to 11.0% for protein across regions and breeds; relative permanent environmental variances within parity ranged from 31.4 to 34.7% for milk, 18.2 to 22.3% for fat, and 28.3 to 29.1% for protein and were similar across regions and breeds. Adjustment for EBV reduced permanent environmental variance across parities and removed cow genetic variance. Relative permanent environmental variances within parity from unadjusted testday yields were nearly identical to those from adjusted test-day yields. For unadjusted test-day yields, heritabilities ranged from 0.19 to 0.30 for milk, 0.13 to 0.15 for fat, and 0.17 to 0.23 for protein. Adjustments for lactation stage, age at milking, previous days open, and days pregnant were estimated from adjusted test-day yields using the same single-trait repeatability models and variance ratios estimated for permanent environment
Research on Correlation Models Between QoS and Application Layer QoE in IoT-NDN Environment

Internet of Things (IoT) is the inter-networking of people, physical devices, vehicles and other objects to collect and exchange data, which has been receiving lots of attention in recent years. Named Data Networking (NDN) is a new paradigm perceived for the next generation network architecture to deal with the explosive growth in the volume of global IoT traffic. Although researches on Quality of Service (QoS) and Quality of Experience (QoE) are popular all the time, correlation models between QoS and QoE remain unexplored in IoT-NDN. In this paper, we propose application layer QoE and discuss correlation models between QoS and application layer QoE in IoT-NDN. Simulation results conclude concrete mapping relations between QoS and application layer QoE, which are useful to take further actions to improve network performance.
Frequency metrology on the 4s(2)S(1/2)-4p(2)P(1/2) transition in Ca-40(+) for a comparison with quasar data

High accuracy frequency metrology on the 4s S-2(1/2)-4p P-2(1/2) transition in calcium ions is performed using laser cooled and crystallized ions in a linear Paul trap. Calibration is performed with a frequency comb laser, resulting in a transition frequency of f = 755 222 766.2 (1.7) MHz. The accuracy presents an improvement of more than one order of magnitude, and will facilitate a comparison with quasar data in a search for a possible change of the fine structure constant on a cosmological time scale.
Mining Electronic Health Records to Extract Patient-Centered Outcomes Following Prostate cancer Treatment

The clinical, granular data in electronic health record (EHR) systems provide opportunities to improve patient care using informatics retrieval methods. However, it is well known that many methodological obstacles exist in accessing data within EHRs. In particular, clinical notes routinely stored in EHR are composed from narrative, highly unstructured and heterogeneous biomedical text. This inherent complexity hinders the ability to perform automated large-scale medical knowledge extraction tasks without the use of computational linguistics methods. The aim of this work was to develop and validate a Natural Language Processing (NLP) pipeline to detect important patient-centered outcomes (PCOs) as interpreted and documented by clinicians in their dictated notes for male patients receiving treatment for localized prostate cancer at an academic medical center.
Exclusion of linkage of the RYR1, CACNA1S, and ATP2A1 genes to recurrent exertional rhabdomyolysis in Thoroughbreds.

OBJECTIVE To determine whether there was genetic linkage between the recurrent exertional rhabdomyolysis (RER) trait in Thoroughbred horse pedigrees and DNA markers in genes (the sarcoplasmic reticulum calcium release channel [RYR1] gene, the sarcoplasmic reticulum calcium ATPase [ATP2A1] gene, and the transverse tubule dihydropyridine receptor-voltage sensor [CACNA1S] gene) that are important in myoplasmic calcium regulation. ANIMALS 34 horses in the University of Minnesota RER resource herd and 62 Thoroughbreds from 3 families of Thoroughbreds outside of the university in which RER-affected status was assigned after 2 or more episodes of ER had been observed. PROCEDURES Microsatellite DNA markers from the RYR1, ATP2A1, and CACNA1S gene loci on equine chromosomes 10, 13, and 30 were identified. Genotypes were obtained for all horses in the 4 families affected by RER, and data were used to test for linkage of these 3 loci to the RER phenotype. RESULTS Analysis of the RYR1, CACNA1S, and ATP2A1 microsatellites excluded a link between those markers and the RER trait. CONCLUSIONS AND CLINICAL RELEVANCE It is likely that the heritable alterations in muscle contractility that are characteristic of RER are caused by a gene that is not yet known to cause related muscle disease in other species.
Immunostimulatory DNA as a vaccine adjuvant

Immunostimulatory DNA containing unmethylated CpG motifs is recognized by Toll-like receptor 9, resulting in the activation of innate immune responses that subsequently amplify the adaptive-immune response. Advances in the characterization of Toll-like receptor 9 signaling have identified immunostimulatory sequences (ISS) with distinct biological activities. Numerous animal models have demonstrated that synthetic ISS are effective adjuvants that enhance both humoral and cellular immune responses in diverse indications, ranging from infectious disease to cancer and allergy. An added benefit supporting the use of ISS as a vaccine adjuvant is that the specific activation of a pathway critical to the regulation of the immune response results in minimal toxicity. To date, clinical testing has largely affirmed the potency and safety of ISS-adjuvanted vaccines.
Potential cIBR-Conjugated PLGA Nanoparticles for Selective Targeting to Leukemic Cells

The expression of LFA-1 diverges from the physiological condition, thus active targeting carr ier can provide the benefits from difference into LFA-1 expression in v arious conditions. Here, the selectivity of cIBR-conjugated nanopartic les (cIBR-NPs), in terms of uptake, was investigated using PBMCs, M ixed PBMCMolt-3 cells and Molt-3 cells. The expressions of L FA-1 on Molt-3 cells, from flow cytometry and Western blot, posses ed the highest level whereas PBMCs showed the lowest level. The ki n tic uptake profiles of cIBR-NPs were obtained by flow cytometr y, which the degree of cellular uptake presented a similar trend with the level of LFA-1 indicating the influence of LFA-1 expression the cellular uptake of cIBR-NPs. The conformation of LFA-1 had a slight effect on the cellular uptake of cIBR-NPs. Overall we demo nstrated that cIBR-NPs enhanced cellular uptake and improved the sel ctivity of drug carriers to LFA-1 on the leukemia cells, which related with the order of LFA-1 expression. Keywords—cIBR, LFA-1, Molt-3, PBMCs
Keap1‐Nrf2 system regulates cell fate determination of hematopoietic stem cells

Nrf2 is a major transcriptional activator of cytoprotective genes against oxidative/electrophilic stress, and Keap1 negatively regulates Nrf2. Emerging works have also suggested a role for Nrf2 as a regulator of differentiation in various cells, but the contribution of Nrf2 to the differentiation of hematopoietic stem cells (HSCs) remains elusive. Clarifying this point is important to understand Nrf2 functions in the development and/or resolution of inflammation. Here, we established two transgenic reporter mouse lines that allowed us to examine Nrf2 expression precisely in HSCs. Nrf2 was abundantly transcribed in HSCs, but its activity was maintained at low levels due to the Keap1‐mediated degradation of Nrf2 protein. When we characterized Keap1‐deficient mice, their bone marrow cells showed enhanced granulocyte‐monocyte differentiation at the expense of erythroid and lymphoid differentiation. Importantly, Keap1‐null HSCs showed lower expression of erythroid and lymphoid genes than did control HSCs, suggesting granulocyte‐monocyte lineage priming in Keap1‐null HSCs. This abnormal lineage commitment was restored by a concomitant deletion of Nrf2, demonstrating the Nrf2‐dependency of the skewing. Analysis of Nrf2‐deficient mice revealed that the physiological level of Nrf2 is sufficient to contribute to the lineage commitment. This study unequivocally shows that the Keap1‐Nrf2 system regulates the cell fate determination of HSCs.
Implementation of V/f scalar control for speed regulation of a three-phase induction motor

This paper presents the experimental implementation of V/f scalar control for a three-phase squirrel-cage induction motor. The principal goal is to control the rotation speed set by a reference and to maintain it despite the presence of disturbances. Two implementation methods were developed: Open-loop scheme, which is commonly used in industry, and closed-loop scheme, less used but it offers higher performance characteristics. MATLAB/Simulink and DS1104 controller board from dSPACE platform were used to carry out real-time experiments and final implementation.
A Specific Gibberellin 20-Oxidase Dictates the Flowering-Runnering Decision in Diploid Strawberry[OPEN]

A gibberellin 20-oxidase plays a pivotal role in transitioning the axillary meristem to a stolon or floral shoot and hence the trade-off between vegetative propagation and fruit yield in strawberry. Asexual and sexual reproduction occur jointly in many angiosperms. Stolons (elongated stems) are used for asexual reproduction in the crop species potato (Solanum tuberosum) and strawberry (Fragaria spp), where they produce tubers and clonal plants, respectively. In strawberry, stolon production is essential for vegetative propagation at the expense of fruit yield, but the underlying molecular mechanisms are unknown. Here, we show that the stolon deficiency trait of the runnerless (r) natural mutant in woodland diploid strawberry (Fragaria vesca) is due to a deletion in the active site of a gibberellin 20-oxidase (GA20ox) gene, which is expressed primarily in the axillary meristem dome and primordia and in developing stolons. This mutation, which is found in all r mutants, goes back more than three centuries. When FveGA20ox4 is mutated, axillary meristems remain dormant or produce secondary shoots terminated by inflorescences, thus increasing the number of inflorescences in the plant. The application of bioactive gibberellin (GA) restored the runnering phenotype in the r mutant, indicating that GA biosynthesis in the axillary meristem is essential for inducing stolon differentiation. The possibility of regulating the runnering-flowering decision in strawberry via FveGA20ox4 provides a path for improving productivity in strawberry by controlling the trade-off between sexual reproduction and vegetative propagation.
Sorafenib targets VCP in hepatocellular cancer cells 1 Sorafenib – mediated targeting of the AAA + ATPase p 97 / VCP leads to disruption of the secretory pathway , endoplasmic reticulum stress and hepatocellular cancer cell death

203 Word count: 5037 Figures: 6 References: 38 on October 28, 2017. © 2012 American Association for Cancer Research. mct.aacrjournals. org Downloaded from Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author Manuscript Published OnlineFirst on October 5, 2012; DOI: 10.1158/1535-7163.MCT-12-0516
FPGA bitstream compression and decompression based on LZ77 algorithm and BMC technique

In this paper, we propose a FPGA bitstream compression method based on LZ77 algorithm and Bitmask-based Compression(BMC) Technique. In order to improve the compression ratio, we optimize LZ77 by encoding the variable `length' with golomb code and simplifying the encoding output. We develop appropriate adding strategy and choose proper cost-benefit parameters of BMC, and combine the BMC with LZ77 to further improve the compression ratio. Experiment results shows that our method has 12.9% improvement in compression ratio over fixed dictionary approach and 9.3% over LZSS on an average. Our compression method also has good compatibility. A decompressor which has two look-ahead buffers is designed for decompression. The pipeline architecture of the decompressor improves the throughput. The decompression throughput reaches 1692Mb/s according to Xilinx ISE report. We have verified the decompressor on a Virtex-5 FPGA.