Miracle-dz/UniFAR-FTUs huggingface dataset:
Behavioral and neurophysiological signatures of interoceptive enhancements following vagus nerve stimulation

An accruing body of research has shown that interoception (the sensing of signals from the body's internal milieu) relies on both a direct route (afforded by the vagus nerve) and a secondary route (supported by somatosensory mechanisms). However, no study has causally tested the differential role of these pathways, let alone via direct stimulation. To bridge this gap, we tested whether multidimensional signatures of interoception are modulated by noninvasive vagus nerve stimulation (nVNS). Sixty‐three participants were divided into an nVNS and a sham‐stimulation group. Before and after stimulation, both groups performed a validated heartbeat detection (HBD) task including a genuinely interoceptive condition (monitoring one's own heartbeat) and a control exteroceptive condition (tracking an aurally presented heartbeat). Electroencephalographic signals were obtained during both conditions to examine modulations of the heartbeat‐evoked potential (HEP). Moreover, before and after stimulation, participants were asked to complete a somatosensory heartbeat localization task. Results from the interoceptive condition revealed that, after treatment, only the nVNS group exhibited improved performance and greater HEP modulations. No behavioral differences were found for the exteroceptive control condition, which was nonetheless associated with significant HEP modulations. Finally, no between‐group differences were observed regarding the localization of the heartbeat sensations or relevant cardiodynamic variables (heart rate and or heart rate variability). Taken together, these results constitute unprecedented evidence that the vagus nerve plays a direct role in neurovisceral integration during interoception. This finding can constrain mechanistic models of the domain while informing a promising transdiagnostic agenda for interoceptive impairments across neuropsychiatric conditions.
The Ketogenic Diet Increases In Vivo Glutathione Levels in Patients with Epilepsy

The Ketogenic Diet (KD) is a high-fat, low-carbohydrate diet that has been utilized as the first line treatment for contrasting intractable epilepsy. It is responsible for the presence of ketone bodies in blood, whose neuroprotective effect has been widely shown in recent years but remains unclear. Since glutathione (GSH) is implicated in oxidation-reduction reactions, our aim was to monitor the effects of KD on GSH brain levels by means of magnetic resonance spectroscopy (MRS). MRS was acquired from 16 KD patients and seven age-matched Healthy Controls (HC). We estimated metabolite concentrations with linear combination model (LCModel), assessing differences between KD and HC with t-test. Pearson was used to investigate GHS correlations with blood serum 3-B-Hydroxybutyrate (3HB) concentrations and with number of weekly epileptic seizures. The results have shown higher levels of brain GSH for KD patients (2.5 ± 0.5 mM) compared to HC (2.0 ± 0.5 mM). Both blood serum 3HB and number of seizures did not correlate with GSH concentration. The present study showed a significant increase in GSH in the brain of epileptic children treated with KD, reproducing for the first time in humans what was previously observed in animal studies. Our results may suggest a pivotal role of GSH in the antioxidant neuroprotective effect of KD in the human brain.
Protein intake and renal function in older patients

Purpose of review Chronic kidney disease (CKD) is highly prevalent in elderly patients. There is growing recognition of the importance of attention to dietary protein intake (DPI) in this population given their predisposition to age-related changes in kidney function and coexisting comorbidities (i.e., hypertension). We reviewed the impact of DPI on kidney health and survival and the role of dietary protein management in older CKD patients. Recent findings While kidney function parameters including glomerular filtration rate (GFR) and renal plasma flow are slightly lower in elderly patients irrespective of CKD status, the kidneys’ ability to compensate for increased DPI by augmentation of GFR is preserved until 80 years of age or less. However, long-term consumption of high DPI in individuals of older age and/or with CKD may contribute to kidney function deterioration over time. Prescription of a plant-dominant low-protein diet of 0.6–0.8 g/kg/day with more than 50% from plant sources or very low protein diets less than 0.45 g/kg/day supplemented with essential amino acids or their keto-analogues may be effective in preserving kidney function in older patients and their younger counterparts, while also monitoring for development of protein–energy wasting (PEW). Summary Using tailored precision nutrition approaches in prescribing plant-dominant low DPI that also maintains adequate energy and nitrogen balance may ameliorate kidney function decline while also preventing development of PEW in elderly patients with CKD.
Of Great Apes and Magpies: Initiations into Animal Behaviour

Simple Summary Animal encounters have been favourite subjects for a long time and it would scarcely be novel to report such stories for their own sake, even though the ones told here are dramatic enough to stand on their own. The questions addressed in this paper are twofold. The first question is: What influence may particular and dramatic animal encounters have on the human observer and how dependent is such a response on previously held attitudes? This paper provides three cases studies of extraordinary moments that changed the lives of the human participants and turned them into advocates of the species they had encountered. The next question asked is how we can be respectful of animals without anthropomorphising them and study them in ways that help us understand their abilities and their needs rather than impose questions that mean much to the human researcher but could be irrelevant to the species? The examples given here compare and contrast species that are especially close to us (great apes) with studies of those that are distant from us in their evolution (birds) and show how different attitudes change the questions that can be asked by scientists, demonstrably leading to new and even stunning results. Abstract This paper presents three case studies of exceptional human encounters with animals. These particular examples were selected because they enabled analysis of the underlying reasons that led the human participants to respond in new ways to their animal counterparts. The question asked here is whether sudden insights into the needs and abilities of an animal arises purely from an anthropocentric position as empathy because of genetic closeness (e.g., chimpanzees) or is something else and whether new insights can be applied to other phylogenetic orders not close to us, e.g., birds, and change research questions and implicit prejudices and stereotypes. Particularly in avian species, phylogenetically distant from humans, the prejudices (anthroprocentric position) and the belief in human uniqueness (human exceptionalism) might be greater than in the reactions to primates. Interestingly, in studies of great apes, contradictory opinions and controversies about cognitive abilities, especially when compared with humans, tend to be pronounced. Species appropriateness in test designs are desirable present and future goals but here it is suggested how different experiences can also lead to different questions that explode the myth of human uniqueness and then arrive at entirely different and new results in cognitive and affective abilities of the species under investigation.
The Role of Protein Disorder in Nuclear Transport and in Its Subversion by Viruses

The transport of host proteins into and out of the nucleus is key to host function. However, nuclear transport is restricted by nuclear pores that perforate the nuclear envelope. Protein intrinsic disorder is an inherent feature of this selective transport barrier and is also a feature of the nuclear transport receptors that facilitate the active nuclear transport of cargo, and the nuclear transport signals on the cargo itself. Furthermore, intrinsic disorder is an inherent feature of viral proteins and viral strategies to disrupt host nucleocytoplasmic transport to benefit their replication. In this review, we highlight the role that intrinsic disorder plays in the nuclear transport of host and viral proteins. We also describe viral subversion mechanisms of the host nuclear transport machinery in which intrinsic disorder is a feature. Finally, we discuss nuclear import and export as therapeutic targets for viral infectious disease.
Effects of Asymmetric Vibration Frequency on Pulling Illusions

It is known that humans experience a haptic illusion, such as the sensation of being pulled in a particular direction, when asymmetric vibrations are presented. A pulling illusion has been used to provide a force feedback for a virtual reality (VR) system and a pedestrian navigation system, and the asymmetric vibrations can be implemented in any small non-grounded device. However, the design methodology of asymmetric vibration stimuli to induce the pulling illusion has not been fully demonstrated. Although the frequency of the asymmetric vibration is important, findings on the frequency have not been reported. In this study, we clarified the influences of the effects on the pulling illusion based on the investigation of asymmetric vibration frequency differences. Two psychophysical experiments that related to the frequency of asymmetric vibration were performed. Experiment I showed that the illusion occurs for specific vibration waveforms at 40 Hz and 75 Hz. As a result of Experiment II, the threshold was the lowest when the frequency was 40 Hz, and highest when the frequency was 110 Hz. This result supports the previous hypothesis that the Meissner corpuscles and the Ruffini endings contribute to the illusion, while the Pacinian corpuscles do not.
Absorptive capacity in the adoption of innovations in health care: a scoping review protocol.

OBJECTIVE To explore how absorptive capacity has been conceptualized and measured in studies of innovation adoption in health care organizations. INTRODUCTION Current literature highlights the need to incorporate knowledge translation processes at the organizational and system level to enhance the adoption of new knowledge into practice. Absorptive capacity is a set of routines and processes characterized by knowledge acquisition, assimilation, transformation, and application. Absorptive capacity, a key concept in organizational learning theory, is thought to be critical to the adoption of new knowledge and innovations in organizations. INCLUSION CRITERIA This scoping review will include primary studies (ie, experimental, quasi-experimental, observational, and qualitative study designs) and grey literature that broadly focus on the adoption of innovations at the organizational level in health care, and frame innovation adoption as processes that rely on organizational learning and absorptive or learning capacity. METHODS Data sources will include comprehensive searches of electronic databases (eg, MEDLINE, Embase, PsycINFO, CINAHL, and Scopus), grey literature, and reference scanning of relevant studies. Study abstracts and full texts will be screened for eligibility by two reviewers, independently. Data extraction of relevant studies will also be done independently by two reviewers. All discrepancies will be addressed through further discussion or adjudicated by a third reviewer. Synthesis of the extracted data will focus on descriptive frequencies, counts, and thematic analysis and the results will be reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension to scoping reviews (PRISMA-ScR).
Human Endometrial Transcriptome and Progesterone Receptor Cistrome Reveal 1 Important Pathways and Epithelial Regulators 2 3

1 Reproductive and Developmental Biology Laboratory, National Institute of Environmental 6 Health Sciences, Research Triangle Park, North Carolina, USA 7 2 Integrative Bioinformatics Support Group, National Institute of Environmental Health Sciences, 8 Research Triangle Park, North Carolina, USA 9 3 Interdepartmental Program in Translational Biology and Molecular Medicine, Baylor College of 10 Medicine, Houston, Texas, USA 11 4 Department of Obstetrics and Gynecology, University of North Carolina at Chapel Hill, Chapel 12 Hill, North Carolina, USA 13 5 Division of Animal Sciences and Department of Obstetrics, Gynecology and Women’s Health, 14 University of Missouri, Columbia, Missouri, USA 15
Evolution of the stroke paradigm: A review of delayed recanalization

While the time window for reperfusion after ischemic stroke continues to increase, many patients are not candidates for reperfusion under current guidelines that allow for reperfusion within 24 h after last known well time; however, many case studies report favorable outcomes beyond 24 h after symptom onset for both spontaneous and medically induced recanalization. Furthermore, modern imaging allows for identification of penumbra at extended time points, and reperfusion risk factors and complications are becoming better understood. Taken together, continued urgency exists to better understand the pathophysiologic mechanisms and ideal setting of delayed recanalization beyond 24 h after onset of ischemia.
The Role of Monoclonal Antibodies in Smoldering and Newly Diagnosed Transplant-Eligible Multiple Myeloma

The recent introduction of monoclonal antibodies (MoAbs), with several cellular targets, such as CD-38 (daratumumab and isatuximab) and SLAM F7 (elotuzumab), differently combined with other classes of agents, has significantly extended the outcomes of patients with multiple myeloma (MM) in different phases of the disease. Initially used in advanced/refractory patients, different MoAbs combination have been introduced in the treatment of newly diagnosed transplant eligible patients (NDTEMM), showing a significant improvement in the depth of the response and in survival outcomes, without a significant price in terms of toxicity. In smoldering MM, MoAbs have been applied, either alone or in combination with other drugs, with the goal of delaying the progression to active MM and restoring the immune system. In this review, we will focus on the main results achieved so far and on the main on-going trials using MoAbs in SMM and NDTEMM.
Pharmacogenetics factors influencing smoking cessation success; the importance of nicotine metabolism

ABSTRACT Introduction: Smoking remains a worldwide epidemic, and despite an increase in public acceptance of the harms of tobacco use, it remains the leading cause of preventable death. It is estimated that up to 70% of all smokers express a desire to quit, but only 3–5% of them are successful. Areas covered: The goal of this review was to evaluate the current status of smoking cessation treatments and the feasibility of implementing personalized-medicine approaches to these pharmacotherapies. We evaluated the genetics associated with higher levels of nicotine addiction and follow with an analysis of the genetic variants that affect the nicotine metabolic ratio (NMR) and the FDA approved treatments for smoking cessation. We also highlighted the gaps in the process of translating current laboratory understanding into clinical practice, and the benefits of personalized treatment approaches for a successful smoking cessation strategy. Expert opinion: Evidence supports the use of tailored therapies to ensure that the most efficient treatments are utilized in an individual’s smoking cessation efforts. An understanding of the genetic effects on the efficacy of individualized smoking cessation pharmacotherapies is key to smoking cessation, ideally utilizing a polygenetic risk score that considers all genetic variation.
An Inside Job: Applications of Intracellular Single Domain Antibodies

Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is characterized by its small size, high solubility and oftentimes exceptional stability. Because of this, most single domain antibodies fold correctly when expressed in the reducing environment of the cytoplasm, and thereby retain their antigen binding specificity. Single domain antibodies can thus be used to target a broad range of intracellular proteins. Such intracellular single domain antibodies are also known as intrabodies, and have proven to be highly useful tools for basic research by allowing visualization, disruption and even targeted degradation of intracellular proteins. Furthermore, intrabodies can be used to uncover prospective new therapeutic targets and have the potential to be applied in therapeutic settings in the future. In this review we provide a brief overview of recent advances in the field of intracellular single domain antibodies, focusing on their use as research tools and potential therapeutic applications. Special attention is given to the available methods that allow delivery of single domain antibodies into cells.
Cooperative pattern formation in multi-species bacterial colonies

Self-organization is a hallmark of all living systems [1]. In particular, coordinated cellular behavior, commonly orchestrated at the population level through reciprocal interactions among different cell species [2-4], regulates the spatial arrangement of specialized cell types to generate tissue patterning and form complex body layouts [5, 6]. The overwhelming complexity of living systems, however, makes deciphering the underlying mechanisms difficult and limits our knowledge of basic pattern-forming mechanism in vivo [7, 8]. A successful strategy is then to work with synthetic, engineered systems, in which cellular interactions can be more easily tailored and studied [9-13]. Here, we demonstrate a simple mechanism through which different populations of cells can self-organize in periodic patterns. Programmed population interactions are shown to lead to coordinated out-ofphase spatial oscillations of two engineered populations of Escherichia coli. Using a combination of experimental and theoretical approaches, we show how such patterns arise autonomously from reciprocal density-dependent activation of cellular motility between the two species, without the need of any preexisting positional or orientational cues. Moreover, by re-designing the interaction, the original out-of-phase spatial oscillation rhythm of the two populations can be accordingly turned into in-phase oscillations. The robustness and versatility of the underlying pattern-formation process suggest that it could both be generically encountered in nature, for instance in the complex bacterial ecosystems found in biofilms [14-16], and used to promote the mixing or demixing of active particles in a controlled way.
Low interest in physical activity and higher rates of obesity among rural teachers.

BACKGROUND Few studies have evaluated the causes of physical inactivity in rural teachers from South America. Determining the causes and effects of physical inactivity in rural teachers could be the base for future intervention studies aiming to improve physical activity (PA) engagement in this population. OBJECTIVES To examine the levels of PA in rural teachers and evaluate the associations between their jobs, interest in PA, body composition and practice of PAMETHODS:Body mass index (BMI) and body fat % (BF) of rural teachers from the Valparaiso Region in Chile were measured; interviews regarding PA habits, interests and PA practice were conducted. The relationship between BF% and interest in PA and the practice of sports was analysed stratifying by sex. RESULTS Around 71% were overweight/obese and 80% of the teachers reported not practicing any PA. These figures are higher than the Chilean national average for adults (64.5% overweight/obese). Moreover, females presented a higher BF% than male teachers (35.98% vs. 26.44%, p < 0.05). The main causes of not performing PA were: firstly, the lack of time (51.8%), and secondly, fatigue after work and/or finishing work late. In addition, rural teachers claim that in their free time they continue to finish tasks that they were not able to complete during their working hours. CONCLUSIONS Rural Chilean teachers show a high prevalence of obesity and low PA levels. Moreover, rural teachers have a work overload stemming from their jobs, and this could be an important link to an overall sedentary lifestyle. Further studies should focus on the relationship between teacher obesity, physical activity, nutrition and work overload.
An industrial system for vehicle tyre detection and text recognition using a pipeline of conventional image processing and deep learning

—This paper presents an industrial system to read text on tyre sidewalls. Images of vehicle tyres in motion are acquired using roadside cameras. Firstly, the tyre circularity is detected using Circular Hough Transform (CHT) with dynamic radius detection. The tyre is then unwarped into a rectangular patch and a cascade of convolutional neural network (CNN) classiﬁers is applied for text recognition. We introduce a novel proposal generator for localizing the tyre code by combining Histogram of Oriented Gradients (HOG) with a CNN. The proposals are then ﬁltered using a deep network. After the code is localized, character detection and recognition are carried out using two separate deep CNNs. The end-to-end system presents impressive accuracy and efﬁciency proving its suitability for the intended industrial application.