Miracle-dz/UniFAR-FTUs huggingface dataset:
Sparsely Distributed, Pre-synaptic Kv3 K+ Channels Control Spontaneous Firing and Cross-Unit Synchrony via the Regulation of Synaptic Noise in an Auditory Brainstem Circuit

Spontaneous subthreshold activity in the central nervous system is fundamental to information processing and transmission, as it amplifies and optimizes sub-threshold signals, thereby improving action potential initiation and maintaining reliable firing. This form of spontaneous activity, which is frequently considered noise, is particularly important at auditory synapses where acoustic information is encoded by rapid and temporally precise firing rates. In contrast, when present in excess, this form of noise becomes detrimental to acoustic information as it contributes to the generation and maintenance of auditory disorders such as tinnitus. The most prominent contribution to subthreshold noise is spontaneous synaptic transmission (synaptic noise). Although numerous studies have examined the role of synaptic noise on single cell excitability, little is known about its pre-synaptic modulation owing in part to the difficulties of combining noise modulation with monitoring synaptic release. Here we study synaptic noise in the auditory brainstem dorsal cochlear nucleus (DCN) of mice and show that pharmacological potentiation of Kv3 K+ currents reduces the level of synaptic bombardment onto DCN principal fusiform cells. Using a transgenic mouse line (SyG37) expressing SyGCaMP2-mCherry, a calcium sensor that targets pre-synaptic terminals, we show that positive Kv3 K+ current modulation decreases calcium influx in a fifth of pre-synaptic boutons. Furthermore, while maintaining rapid and precise spike timing, positive Kv3 K+ current modulation increases the synchronization of local circuit neurons by reducing spontaneous activity. In conclusion, our study identifies a unique pre-synaptic mechanism which reduces synaptic noise at auditory synapses and contributes to the coherent activation of neurons in a local auditory brainstem circuit. This form of modulation highlights a new therapeutic target, namely the pre-synaptic bouton, for ameliorating the effects of hearing disorders which are dependent on aberrant spontaneous activity within the central auditory system.
Mechano-Hypoxia Conditioning of Engineered Human Meniscus

Meniscus fibrochondrocytes (MFCs) experience simultaneous hypoxia and mechanical loading in the knee joint. Experimental conditions based on these aspects of the native MFC environment may have promising applications in human meniscus tissue engineering. We hypothesized that in vitro “mechano-hypoxia conditioning” with mechanical loading such as dynamic compression (DC) and cyclic hydrostatic pressure (CHP) would enhance development of human meniscus fibrocartilage extracellular matrix in vitro. MFCs from inner human meniscus surgical discards were pre-cultured on porous type I collagen scaffolds with TGF-β3 supplementation to form baseline tissues with newly formed matrix that were used in a series of experiments. First, baseline tissues were treated with DC or CHP under hypoxia (HYP, 3% O2) for 5 days. DC was the more effective load regime in inducing gene expression changes, and combined HYP/DC enhanced gene expression of fibrocartilage precursors. The individual treatments of DC and HYP regulated thousands of genes, such as chondrogenic markers SOX5/6, in an overwhelmingly additive rather than synergistic manner. Similar baseline tissues were then treated with a short course of DC (5 vs 60 min, 10–20% vs 30–40% strain) with different pre-culture duration (3 vs 6 weeks). The longer course of loading (60 min) had diminishing returns in regulating mechano-sensitive and inflammatory genes such as c-FOS and PTGS2, suggesting that as few as 5 min of DC was adequate. There was a dose-effect in gene regulation by higher DC strains, whereas outcomes were inconsistent for different MFC donors in pre-culture durations. A final set of baseline tissues was then cultured for 3 weeks with mechano-hypoxia conditioning to assess mechanical and protein-level outcomes. There were 1.8–5.1-fold gains in the dynamic modulus relative to baseline in HYP/DC, but matrix outcomes were equal or inferior to static controls. Long-term mechano-hypoxia conditioning was effective in suppressing hypertrophic markers (e.g., COL10A1 10-fold suppression vs static/normoxia). Taken together, these results indicate that appropriately applied mechano-hypoxia conditioning can support meniscus fibrocartilage development in vitro and may be useful as a strategy for developing non-hypertrophic articular cartilage using mesenchymal stem cells.
How Does Leader Empowering Behavior Promote Employee Knowledge Sharing? The Perspective of Self-Determination Theory

Although scholars have recognized the important role of leader empowering behavior in promoting employee knowledge sharing, investigations on the potential underlying mechanism are still limited. To enrich studies revealing the possible underlying paths, drawing on self-determination theory, this paper proposes a moderated mediation model. We propose that employee self-determination plays a mediating role and employee proactivity moderates the mediating effect. We test our hypotheses based on data collected from 230 employees across a three-wave study. The empirical results demonstrate that leader empowering behavior promotes employee knowledge sharing by enhancing employee self-determination. Employees’ proactive personality moderates the indirect effect such that the indirect effect is stronger when employees have high levels of proactive personality. This paper thus contributes to the related literature and reveals practical implications.
Replication of the Mammalian Genome by Replisomes Specific for Euchromatin and Heterochromatin

Replisomes follow a schedule in which replication of DNA in euchromatin is early in S phase while sequences in heterochromatin replicate late. Impediments to DNA replication, referred to as replication stress, can stall replication forks triggering activation of the ATR kinase and downstream pathways. While there is substantial literature on the local consequences of replisome stalling–double strand breaks, reversed forks, or genomic rearrangements–there is limited understanding of the determinants of replisome stalling vs. continued progression. Although many proteins are recruited to stalled replisomes, current models assume a single species of “stressed” replisome, independent of genomic location. Here we describe our approach to visualizing replication fork encounters with the potent block imposed by a DNA interstrand crosslink (ICL) and our discovery of an unexpected pathway of replication restart (traverse) past an intact ICL. Additionally, we found two biochemically distinct replisomes distinguished by activity in different stages of S phase and chromatin environment. Each contains different proteins that contribute to ICL traverse.
Implementation of a Commitment Machine for an Adaptive and Robust Expected Shortfall Estimation

This study proposes a metaheuristic for the selection of models among different Expected Shortfall (ES) estimation methods. The proposed approach, denominated “Commitment Machine” (CM), has a strong focus on assets cross-correlation and allows to measure adaptively the ES, dynamically evaluating which is the most performing method through the minimization of a loss function. The CM algorithm compares four different ES estimation techniques which all take into account the interaction effects among assets: a Bayesian Vector autoregressive model, Stochastic Differential Equation (SDE) numerical schemes with Exponential Weighted Moving Average (EWMA), a Generalized AutoRegressive Conditional Heteroskedasticity (GARCH) volatility model and a hybrid method that integrates Dynamic Recurrent Neural Networks together with a Monte Carlo approach. The integration of traditional Monte Carlo approaches with Machine Learning technologies and the heterogeneity of dynamically selected methodologies lead to an improved estimation of the ES. The study describes the techniques adopted by the CM and the logic behind model selection; moreover, it provides a market application case of the proposed metaheuristic, by simulating an equally weighted multi-asset portfolio.
Devenir de la protéine insecticide Cry1Aa issue de Bacillus thuringiensis (Bt) dans le sol

Depuis leur commercialisation en 1996, les surfaces de cultures de plantes transgeniques Bt ont considerablement augmentees, representant en 2007 pres de 42 millions d'hectares. Ces plantes produisent en continu une proteine insecticide (issue de Bacillus thuringiensis) leur permettant de lutter contre les insectes ravageurs tels que la pyrale du mais. La toxine est introduite dans les sols par exsudation racinaire et degradation des tissus vegetaux. Les interactions de la toxine avec les particules de sol peuvent modifier sa mobilite, sa biodisponibilite, sa persistance et sa toxicite. Il est donc important d'etudier le role du sol dans le devenir de cette toxine. Ce travail de these a permis de mieux comprendre les interactions physicochimiques entre la toxine et les composantes du sol et en particulier avec les argiles, surfaces tres reactives. L'adsorption de la toxine sur les argiles est une interaction de faible affinite, fortement dependante du pH et difficilement reversible. La difference de quantite maximale de toxine adsorbee sur la montmorillonite et sur la kaolinite etait fortement liee a leurs surfaces specifiques respectives, plutot qu'a leur charge de surface specifique. Une analyse de la mobilite de la toxine a l'etat adsorbe sur la montmorillonite par Fluorescence Recovery After Photobleaching (FRAP) a montre que cette proteine est immobilisee par son adsorption sur l'argile. Ces resultats suggerent que le risque de transfert de la toxine dans le sol se limite aux transports facilites par les colloides et aux processus de bioturbation. Par ailleurs, des etudes de persistance de la toxine dans le sol ont montre que plus de 50 % de l'immuno-reactivite de la toxine est perdue en moins d'une semaine. Les processus biotiques (degradations microbiennes) semblent peu impliques dans ce phenomene. Il semblerait que la toxine ne soit pas degradee mais plutot inactivee par des changements de conformations suite a son interaction avec les composants du sol. Les processus abiotiques (interactions physicochimiques) sont donc fortement impliques dans la persistance de la toxine dans le sol, avec une contribution significative des interactions hydrophobes. Dans ce travail de these, un effort important a ete consacre a l'elaboration d'une methode de detection in situ de la toxine dans le sol. Deux strategies ont ete envisagees, basees a la fois sur la spectroscopie de fluorescence et l'immunologie, mettant en jeu la reconnaissance soit de deux epitopes de la toxine par deux anticorps marques (Fluorescence Resonance Energy Transfer, FRET), soit la reconnaissance d'un epitope par un anticorps marque apres presaturation des surfaces (sites proteiques). Les resultats, encore preliminaires, sont encourageants et necessitent d'etre poursuivis.
Improvement of Phosphorus Use Efficiency in Rice by Adopting Image-Based Phenotyping and Tolerant Indices

Phosphorus is one of the second most important nutrients for plant growth and development, and its importance has been realised from its role in various chains of reactions leading to better crop dynamics accompanied by optimum yield. However, the injudicious use of phosphorus (P) and non-renewability across the globe severely limit the agricultural production of crops, such as rice. The development of P-efficient cultivar can be achieved by screening genotypes either by destructive or non-destructive approaches. Exploring image-based phenotyping (shoot and root) and tolerant indices in conjunction under low P conditions was the first report, the epicentre of this study. Eighteen genotypes were selected for hydroponic study from the soil-based screening of 68 genotypes to identify the traits through non-destructive (geometric traits by imaging) and destructive (morphology and physiology) techniques. Geometric traits such as minimum enclosing circle, convex hull, and calliper length show promising responses, in addition to morphological and physiological traits. In 28-day-old seedlings, leaves positioned from third to fifth played a crucial role in P mobilisation to different plant parts and maintained plant architecture under P deficient conditions. Besides, a reduction in leaf angle adjustment due to a decline in leaf biomass was observed. Concomitantly, these geometric traits facilitate the evaluation of low P-tolerant rice cultivars at an earlier stage, accompanying several stress indices. Out of which, Mean Productivity Index, Mean Relative Performance, and Relative Efficiency index utilising image-based traits displayed better responses in identifying tolerant genotypes under low P conditions. This study signifies the importance of image-based phenotyping techniques to identify potential donors and improve P use efficiency in modern rice breeding programs.
Gut Microbiota Associated With Different Sea Lamprey (Petromyzon marinus) Life Stages

Sea lamprey (SL; Petromyzon marinus), one of the oldest living vertebrates, have a complex metamorphic life cycle. Following hatching, SL transition into a microphagous, sediment burrowing larval stage, and after 2–10+ years, the larvae undergo a dramatic metamorphosis, transforming into parasitic juveniles that feed on blood and bodily fluids of fishes; adult lamprey cease feeding, spawn, and die. Since gut microbiota are critical for the overall health of all animals, we examined the microbiota associated with SLs in each life history stage. We show that there were significant differences in the gut bacterial communities associated with the larval, parasitic juvenile, and adult life stages. The transition from larval to the parasitic juvenile stage was marked with a significant shift in bacterial community structure and reduction in alpha diversity. The most abundant SL-associated phyla were Proteobacteria, Fusobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria, and Firmicutes, with their relative abundances varying among the stages. Moreover, while larval SL were enriched with unclassified Fusobacteriaceae, unclassified Verrucomicrobiales and Cetobacterium, members of the genera with fastidious nutritional requirements, such as Streptococcus, Haemophilus, Cutibacterium, Veillonella, and Massilia, were three to four orders of magnitude greater in juveniles than in larvae. In contrast, adult SLs were enriched with Aeromonas, Iodobacter, Shewanella, and Flavobacterium. Collectively, our findings show that bacterial communities in the SL gut are dramatically different among its life stages. Understanding how these communities change over time within and among SL life stages may shed more light on the role that these gut microbes play in host growth and fitness.
Genome-Wide Single Nucleotide Polymorphism Analysis Elucidates the Evolution of Prunus takesimensis in Ulleung Island: The Genetic Consequences of Anagenetic Speciation

Of the two major speciation modes of endemic plants on oceanic islands, cladogenesis and anagenesis, the latter has been recently emphasized as an effective mechanism for increasing plant diversity in isolated, ecologically homogeneous insular settings. As the only flowering cherry occurring on Ulleung Island in the East Sea (concurrently known as Sea of Japan), Prunus takesimensis Nakai has been presumed to be derived through anagenetic speciation on the island. Based on morphological similarities, P. sargentii Rehder distributed in adjacent continental areas and islands has been suggested as a purported continental progenitor. However, the overall genetic complexity and resultant non-monophyly of closely related flowering cherries have hindered the determination of their phylogenetic relationships as well as the establishment of concrete continental progenitors and insular derivative relationships. Based on extensive sampling of wild flowering cherries, including P. takesimensis and P. sargentii from Ulleung Island and its adjacent areas, the current study revealed the origin and evolution of P. takesimensis using multiple molecular markers. The results of phylogenetic reconstruction and population genetic structure analyses based on single nucleotide polymorphisms detected by multiplexed inter-simple sequence repeat genotyping by sequencing (MIG-seq) and complementary cpDNA haplotypes provided evidence for (1) the monophyly of P. takesimensis; (2) clear genetic differentiation between P. takesimensis (insular derivative) and P. sargentii (continental progenitor); (3) uncertain geographic origin of P. takesimensis, but highly likely via single colonization from the source population of P. sargentii in the Korean Peninsula; (4) no significant reduction in genetic diversity in anagenetically derived insular species, i.e., P. takesimensis, compared to its continental progenitor P. sargentii; (5) no strong population genetic structuring or geographical patterns in the insular derivative species; and (6) MIG-seq method as an effective tool to elucidate the complex evolutionary history of plant groups.
A Study on Green Advertising Effectiveness in the Perspective of Image Proximity

Based on the construal level theory (CLT), this study discusses the effects of congruence between image proximity and product type on advertising attitude and product attitude from the perspective of spatial distance and investigates the mediating role of mental imagery. Data are collected using two laboratory experiments and one online experiment. A two-way ANOVA is used to test the interaction between image proximity and product type, and a bootstrap analysis is used to test the mediating role of mental imagery. The result shows that: (1) For search products, compared with full-length shots, the close-up shots of environmental information can enable consumers to generate more positive advertising attitude and product attitude. For experience products, the full-length shots of environmental information can enable consumers to generate more positive advertising attitude and product attitude than the close-up shots. (2) The congruence effect of image proximity and product type has an impact on advertising attitude and product attitude through mental imagery. This research uses different kinds of image proximity to express environmental information about green products and tries to interpret the effectiveness of green advertisements from a new perspective.
Influenza Viruses: Innate Immunity and mRNA Vaccines

The innate immune system represents the first line of defense against influenza viruses, which cause severe inflammation of the respiratory tract and are responsible for more than 650,000 deaths annually worldwide. mRNA vaccines are promising alternatives to traditional vaccine approaches due to their safe dosing, low-cost manufacturing, rapid development capability, and high efficacy. In this review, we provide our current understanding of the innate immune response that uses pattern recognition receptors to detect and respond to mRNA vaccination. We also provide an overview of mRNA vaccines, and discuss the future directions and challenges in advancing this promising therapeutic approach.
Growth Hormone Overexpression Induces Hyperphagia and Intestinal Morphophysiological Adaptations to Improve Nutrient Uptake in Zebrafish

The excess of circulating growth hormone (GH) in most transgenic animals implies mandatory growth resulting in higher metabolic demand. Considering that the intestine is the main organ responsible for the digestion, absorption, and direction of dietary nutrients to other tissues, this study aimed to investigate the mechanisms by which gh overexpression modulates the intestine to support higher growth. For this purpose, we designed an 8-weeks feeding trial to evaluate growth parameters, feed intake, and intestinal morphometric indices in the adult gh-transgenic zebrafish (Danio rerio) model. To access the sensitivity of the intestine to the excess of circulating GH, the messenger RNA (mRNA) expression of intestine GH receptors (GHRs) (ghra and ghrb) was analyzed. In addition, the expression of insulin-like growth factor 1a (igf1a) and genes encoding for di and tripeptide transporters (pept1a and pept1b) were assessed. Gh-transgenic zebrafish had better growth performance and higher feed intake compared to non-transgenic sibling controls. Chronic excess of GH upregulates the expression of its cognate receptor (ghrb) and the main growth factor related to trophic effects in the intestine (igf1a). Moreover, transgenic zebrafish showed an increased intestinal absorptive area and higher expression of crucial genes related to the absorption of products from meal protein degradation. These results reinforce the ability of GH to modulate intestinal morphology and the mechanisms of assimilation of nutrients to sustain the energy demand for the continuous growth induced by the excess of circulating GH. Graphical Abstract GH overexpression increases the intestinal mass and absorptive surface area both through the direct effects of GH transgenics (up-regulating the expression of its cognate receptor and insulin-like growth factor, igf1a) and through indirect effects through an increase in the amount of food consumed. In addition, hyperphagia provides a greater amount of nutrients in the intestinal lumen and stimulates the synthesis of di- and tri-peptide transporters, which are the main route of absorption of dietary products from protein degradation.
Association Between Endocrine Markers, Accumulated Workload, and Fitness Parameters During a Season in Elite Young Soccer Players

The purpose of this study was to analyze differences between endocrine markers in soccer players, based on playing positions, and correlations between endocrine markers (testosterone, cortisol, growth hormone, and insulin-like growth factor-1), with accumulated workload training and fitness parameters [maximal oxygen uptake (VO2max), countermovement jump (CMJ), and isometric maximal strength (1-RM) of the knee for hamstring (ISH) and quadriceps (ISQ) muscles] during early-, mid-, and end-seasons. Twenty-four elite soccer players under 17 participated in this study. The results showed that there was no difference between levels of the endocrine markers among the different positions of the players. Significant correlations were observed between endocrines parameters and fitness performance (ISQ, ISH, VO2max, and CMJ). Regression analysis showed that 1-RM and VO2max were the best predictors of endocrine markers. These findings demonstrated that the activity profiles of youth soccer players were not influenced by endocrine markers. Also, it may be assumed that endocrines levels can be used to better explain the physical capacities of this population. Finally, endocrines markers may help to predict changes in 1-RM and VO2max.