Miracle-dz/UniFAR-FTUs huggingface dataset:
Improved Scar Outcomes with Increased Daily Duration of Pressure Garment Therapy.

OBJECTIVE Despite the development of a number of treatment modalities, scarring remains common post burn injury. To reduce burn scarring, pressure garment therapy has been widely utilized but is complicated by low patient adherence. To improve adherence, reduced hours of daily garment wear has been proposed. APPROACH To examine the efficacy of pressure garment therapy at reduced durations of daily wear, a porcine burn-excise-autograft model was utilized. Grafted burns were treated with pressure garments (20 mmHg) for 8, 16 or 24 hours of daily wear with untreated burns serving as controls. Scar area, thickness, biomechanical properties and tissue structure were assessed over time. RESULTS All treatment groups reduced scar thickness and contraction versus controls and improved scar pliability and elasticity. Pressure garments worn 24 hours per day significantly reduced contraction versus the 8- and 16-hour groups and prevented alignment of collagen within the dermis. INNOVATION Though pressure garment therapy is prescribed for use 23 hours per day, the need for almost continuous use has not been previously examined. Adjustable, low-fatigue pressure garments were developed for this porcine study to examine the role of daily duration of wear without confounding factors such as garment fatigue and patient adherence. CONCLUSION For maximum efficacy, pressure garments should be worn 23 to 24 hours per day; however, garments worn as little as 8 hours per day significantly improve scar outcomes versus no treatment.
International conference on physical activity and obesity in children : summary statement and recommendations 1 , 2

The increasing prevalence of obesity among the world’s children and youth was the impetus for an international conference convened in Toronto, Canada, to examine issues related to physical activity and obesity in children (24– 27 June 2007). The goal of the conference was to assimilate, interpret, and share scientific evidence with key stakeholders to develop recommendations concerning effective physical activity policies and programs to address obesity in children. The conference was attended by approximately 1000 delegates from 33 countries who gathered to listen to the invited speakers and to share information on promising practices related to the promotion of physical activity with the aim of reducing the burden of obesity in children. The major topics addressed at the conference included the biological and behavioural causes of obesity, current and past levels of physical activity and sedentarism in children, the role of the social, family, and built environments in addressing the physical activity deficit, and the role of legislation and industry in promoting physical activity. Promising physical activity interventions among children were presented, and important research, policy, and practice recommendations to address the issue of physical inactivity and obesity were provided.
Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation

Abstract We examined the effect of ice‐binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP‐treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.
Progress towards a representative network of Southern Ocean protected areas

Global threats to ocean biodiversity have generated a worldwide movement to take actions to improve conservation and management. Several international initiatives have recommended the adoption of marine protected areas (MPAs) in national and international waters. National governments and the Commission for the Conservation of Antarctic Marine Living Resources have successfully adopted multiple MPAs in the Southern Ocean despite the challenging nature of establishing MPAs in international waters. But are these MPAs representative of Southern Ocean biodiversity? Here we answer this question for both existing and proposed Antarctic MPAs, using benthic and pelagic regionalizations as a proxy for biodiversity. Currently about 11.98% of the Southern Ocean is protected in MPAs, with 4.61% being encompassed by no-take areas. While this is a relatively large proportion of protection when compared to other international waters, current Antarctic MPAs are not representative of the full range of benthic and pelagic ecoregions. Implementing additional protected areas, including those currently under negotiation, would encompass almost 22% of the Southern Ocean. It would also substantially improve representation with 17 benthic and pelagic ecoregions (out of 23 and 19, respectively) achieving at least 10% representation.
IKBKE activity enhances AR levels in advanced prostate cancer via modulation of the Hippo pathway

Abstract Resistance to androgen receptor (AR) targeting therapeutics in prostate cancer (PC) is a significant clinical problem. Mechanisms by which this is accomplished include AR amplification and expression of AR splice variants, demonstrating that AR remains a key therapeutic target in advanced disease. For the first time we show that IKBKE drives AR signalling in advanced PC. Significant inhibition of AR regulated gene expression was observed upon siRNA-mediated IKBKE depletion or pharmacological inhibition due to inhibited AR gene expression in multiple cell line models including a LNCaP derivative cell line resistant to the anti-androgen, enzalutamide (LNCaP-EnzR). Phenotypically, this resulted in significant inhibition of proliferation, migration and colony forming ability suggesting that targeting IKBKE could circumvent resistance to AR targeting therapies. Indeed, pharmacological inhibition in the CWR22Rv1 xenograft mouse model reduced tumour size and enhanced survival. Critically, this was validated in patient-derived explants where enzymatic inactivation of IKBKE reduced cell proliferation and AR expression. Mechanistically, we provide evidence that IKBKE regulates AR levels via Hippo pathway inhibition to reduce c-MYC levels at cis-regulatory elements within the AR gene. Thus, IKBKE is a therapeutic target in advanced PC suggesting repurposing of clinically tested IKBKE inhibitors could be beneficial to castrate resistant PC patients.
Ectomycorrhizal fungi drive positive phylogenetic plant-soil feedbacks in a regionally dominant tropical plant family.

While work in temperate forests suggests that there are consistent differences in plant-soil feedback (PSF) between plants with arbuscular and ectomycorrhizal associations, it is unclear whether these differences exist in tropical rainforests. We tested the effects of mycorrhizal type, phylogenetic relationships to overstory species, and soil fertility on the growth of tree seedlings in a tropical Bornean rainforest with a high diversity of both ectomycorrhizal and arbuscular mycorrhizal trees. We found that ectomycorrhizal tree seedlings had higher growth in soils conditioned by close relatives, and that this was associated with higher mycorrhizal colonization. By contrast, arbuscular mycorrhizal tree seedlings generally grew more poorly in soils conditioned by close relatives. For ectomycorrhizal species, the phylogenetic trend was insensitive to soil fertility. For arbuscular mycorrhizal seedlings, however, the effect of growing in soils conditioned by close relatives became increasingly negative as soil fertility increased. Our results demonstrate consistent effects of mycorrhizal type on plant-soil feedbacks across forest biomes. The positive effects of ectomycorrhizal symbiosis may help explain biogeographic variation across tropical forests, such as familial dominance of the Dipterocarpaceae in SE Asia. However, positive feedbacks also raise questions about the role of PSFs in maintaining tropical diversity.
Escherichia coli O157:H7 F9 Fimbriae Recognize Plant Xyloglucan and Elicit a Response in Arabidopsis thaliana

Fresh produce is often a source of enterohaemorrhagic Escherichia coli (EHEC) outbreaks. Fimbriae are extracellular structures involved in cell-to-cell attachment and surface colonisation. F9 (Fml) fimbriae have been shown to be expressed at temperatures lower than 37 °C, implying a function beyond the mammalian host. We demonstrate that F9 fimbriae recognize plant cell wall hemicellulose, specifically galactosylated side chains of xyloglucan, using glycan arrays. E. coli expressing F9 fimbriae had a positive advantage for adherence to spinach hemicellulose extract and tissues, which have galactosylated oligosaccharides as recognized by LM24 and LM25 antibodies. As fimbriae are multimeric structures with a molecular pattern, we investigated whether F9 fimbriae could induce a transcriptional response in model plant Arabidopsis thaliana, compared with flagella and another fimbrial type, E. coli common pilus (ECP), using DNA microarrays. F9 induced the differential expression of 435 genes, including genes involved in the plant defence response. The expression of F9 at environmentally relevant temperatures and its recognition of plant xyloglucan adds to the suite of adhesins EHEC has available to exploit the plant niche.
The effect of ecological factors on eye morphology in the western rainbowfish, Melanotaenia australis

ABSTRACT Ecological factors such as spatial habitat complexity and diet can explain variation in visual morphology, but few studies have sought to determine whether visual specialisation can occur among populations of the same species. We used a small Australian freshwater fish (the western rainbowfish, Melanotaenia australis) to determine whether populations showed variation in eye size and eye position, and whether this variation could be explained by environmental (light availability, turbidity) and ecological (predation risk, habitat complexity, invertebrate abundance) variables. We investigated three aspects of eye morphology – (1) eye size relative to body size, (2) pupil size relative to eye size and (3) eye position in the head – for fish collected from 14 sites in a major river catchment in northwest Western Australia. We found significant variation among populations in all three measures of eye morphology, but no effect of sex on eye size or eye position. Variation in eye diameter and eye position was best explained by the level of habitat complexity. Specifically, fish occurring in habitats with low complexity (i.e. open water) tended to have smaller, more dorsally located eyes than those occurring in more complex habitats (i.e. vegetation present). The size of the pupil relative to the size of the eye was most influenced by the presence of surrounding rock formations; fish living in gorge habitats had significantly smaller pupils (relative to eye size) than those occupying semi-gorge sites or open habitats. Our findings reveal that different ecological and environmental factors contribute to habitat-specific visual specialisations within a species. Summary: The gross morphology and position of the eye in a freshwater fish differs among populations according to light availability and the complexity of the habitat.
Development of a Real-time Indoor Location System using Bluetooth Low Energy Technology and Deep Learning to Facilitate Clinical Applications.

PURPOSE An indoor, real-time location system (RTLS) can benefit both hospitals and patients by improving clinical efficiency through data-driven optimization of procedures. Bluetooth-based RTLS systems are cost-effective but lack accuracy because Bluetooth signal is subject to significant fluctuation. We aim to improve the accuracy of RTLS using the deep learning technique. METHODS We installed a Bluetooth sensor network in a 3-floor clinic building to track patients, staff, and devices. The Bluetooth sensors measured the strength of the signal broadcasted from Bluetooth tags, which was fed into a deep neural network to calculate the location of the tags. The proposed deep neural network consists of a Long Short-Term Memory (LSTM) network and a deep classifier for tracking moving objects. Additionally, a spatial-temporal constraint algorithm was implemented to further increase the accuracy and stability of the results. To train the neural network, we divided the building into 115 zones and collected training data in each zone. We further augmented the training data to generate cross-zone trajectories, mimicking the real-world scenarios. We tuned the parameters for the proposed neural network to achieve relatively good accuracy. RESULTS The proposed deep neural network achieved an overall accuracy of about 97% for tracking objects in each individual zone in the whole 3-floor building, 1.5% higher than the baseline neural network that was proposed in an earlier paper, when using 10 seconds of signals. The accuracy increased with the density of Bluetooth sensors. For tracking moving objects, the proposed neural network achieved stable and accurate results. When latency is less of a concern, we eliminated the effect of latency from the accuracy and gained an accuracy of 100% for our testing trajectories, significantly improved from the baseline method. CONCLUSIONS The proposed deep neural network composed of a LSTM, a deep classifier and a posterior constraint algorithm significantly improved the accuracy and stability of RTLS for tracking moving objects.
Context as a Facilitator of the Implementation of Evidence-based Nursing: A Meta-synthesis

Nurses have numerous difficulties in implementing science due to obstacles related to the work context. The aim is to explore the work-context-related facilitators of the application of evidence in clinical practice by nursing professionals. Qualitative meta-synthesis of primary studies on nurses’ experiences of work-context-related facilitators, as defined by the Promoting Action on Research Implementation in Health Services model. Using the Qualitative Appraisal and Review Instrument of the Joanna Briggs Institute. Of the total 57 primary research articles included, an explanatory model of the facilitating factors related to the work context was generated on the basis of four general categories: institutional support (leadership), multidisciplinary support (teamwork and communication), culture of improving quality of care (nursing professionals’ attitudes towards change) and use of research (valuing research). Action can be taken on the facilitating factors of the evidence-based practice application in nursing clinical environments, providing resources and motivation from the organization.
Understanding the role of configural processing in face emotion recognition in Parkinson's disease.

This investigation examined whether impairment in configural processing could explain deficits in face emotion recognition in people with Parkinson's disease (PD). Stimuli from the Radboud Faces Database were used to compare recognition of four negative emotion expressions by older adults with PD (n = 16) and matched controls (n = 17). Participants were tasked with categorizing emotional expressions from upright and inverted whole faces and facial composites; it is difficult to derive configural information from these two types of stimuli so featural processing should play a larger than usual role in accurate recognition of emotional expressions. We found that the PD group were impaired relative to controls in recognizing anger, disgust and fearful expressions in upright faces. Then, consistent with a configural processing deficit, participants with PD showed no composite effect when attempting to identify facial expressions of anger, disgust and fear. A face inversion effect, however, was observed in the performance of all participants in both the whole faces and facial composites tasks. These findings can be explained in terms of a configural processing deficit if it is assumed that the disruption caused by facial composites was specific to configural processing, whereas inversion reduced performance by making it difficult to derive both featural and configural information from faces.
One-Step Photoactivation of a Dual-Functionalized Bioink as Cell Carrier and Cartilage-Binding Glue for Chondral Regeneration

Cartilage defects can result in pain, disability, and osteoarthritis. Hydrogels providing a chondroregeneration‐permissive environment are often mechanically weak and display poor lateral integration into the surrounding cartilage. This study develops a visible‐light responsive gelatin ink with enhanced interactions with the native tissue, and potential for intraoperative bioprinting. A dual‐functionalized tyramine and methacryloyl gelatin (GelMA‐Tyr) is synthesized. Photo‐crosslinking of both groups is triggered in a single photoexposure by cell‐compatible visible light in presence of tris(2,2′‐bipyridyl)dichlororuthenium(II) and sodium persulfate as initiators. Neo‐cartilage formation from embedded chondroprogenitor cells is demonstrated in vitro, and the hydrogel is successfully applied as bioink for extrusion‐printing. Visible light in situ crosslinking in cartilage defects results in no damage to the surrounding tissue, in contrast to the native chondrocyte death caused by UV light (365–400 nm range), commonly used in biofabrication. Tyramine‐binding to proteins in native cartilage leads to a 15‐fold increment in the adhesive strength of the bioglue compared to pristine GelMA. Enhanced adhesion is observed also when the ink is extruded as printable filaments into the defect. Visible‐light reactive GelMA‐Tyr bioinks can act as orthobiologic carriers for in situ cartilage repair, providing a permissive environment for chondrogenesis, and establishing safe lateral integration into chondral defects.
Multicenter Clinical Evaluation of the Revogene Strep A Molecular Assay for Detection of Streptococcus pyogenes from Throat Swab Specimens

Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-time PCR assay for GAS detection from throat swab specimens within approximately 70 min. This multicenter prospective study evaluated the performance of the Revogene Strep A molecular assay compared to that of bacterial culture. ABSTRACT Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-time PCR assay for GAS detection from throat swab specimens within approximately 70 min. This multicenter prospective study evaluated the performance of the Revogene Strep A molecular assay compared to that of bacterial culture. Dual throat swab specimens in either liquid Amies or Stuart medium were collected from eligible subjects (pediatric population and adults) enrolled across 7 sites (USA and Canada). Revogene Strep A and reference testing was performed within 7 days and 48 h of sample collection, respectively. Of the 604 evaluable specimens, GAS was detected in 154 (25.5%) samples by the reference method and in 175 (29%) samples by the Revogene Strep A assay. Revogene Strep A assay sensitivity and specificity were reported to be 98.1% (95% confidence interval [CI], 94.4 to 99.3) and 94.7% (95% CI, 92.2 to 96.4), respectively. The positive predictive value was 86.3% (95% CI, 80.4 to 90.6), negative predictive value was 99.3% (95% CI, 98.0 to 99.8) with a 1.0% invalid rate. Discrepant analysis with alternative PCR/bidirectional sequencing was performed for 24 false-positive (FP) and 3 false-negative (FN) specimens. Concordant results were reported for 17 (FP only) of 27 discordant specimens. The Revogene Strep A assay had high sensitivity and specificity for GAS detection and provides a faster alternative for GAS diagnosis.
Improving a native pasture with the legume Arachis pintoi in the humid tropics of México

The objective of this study was to determine the effect of introducing the legume Arachis pintoi CIAT 17434 into a native pasture where native grasses dominated the botanical composition, on establishment, persistence, standing dry matter, botanical composition, soil variables, animal performance, herbage quality and ingestive behaviour of the animals, from 1998 to 2001 in the humid tropics of the east coast of Mexico. The treatments were the native grass pasture (NG) as control and NG associated with A. pintoi (NG+Ap), which were grazed by dual purpose F1 (Holstein x Zebu) cows. The most successful type of establishment was vegetative planting of alternated strips of legume and grass in a 1:2 ratio in area. The legume showed a strong stoloniferous habit and accumulation of a high soil seed reserves that allowed its propagation over almost half the pasture area by the end of the study. The SDM was always higher for NG+Ap but the difference among treatments decreased with time. The higher SDM and the effective N 2 fixation allowed the accumulation of C and N in the NG+Ap soil, and in both treatments the bulk density of the soil remained unchanged with time. There were no differences in saleable milk yield (6 kg/cow/day) and calf production per cow (0.5 kg/calf/day) between treatments. Herbage digestibility and crude protein were higher in the association, but this did not reflect in higher animal performance because of low organic matter intakes. Grazing and ruminating times were lower in the association, but biting rates were higher. The farmer may use to his advantage the increase in soil fertility, since it would allow the introduction of more productive exotic grasses or the cultivation of crops without the need of costly N fertilisation.
Clause-initial AND usage in a cross-sectional and longitudinal corpus of school-age children's narratives

Abstract Young children adopt an event-chaining strategy when storytelling, frequently linking clauses with and. The current study tested whether age-related changes in clause-initial and usage might index narrative structure development in the Eugene Children's Story Corpus (ECSC), which includes 180 structured spontaneous narratives elicited yearly for three years from 60 children, aged five to seven at study onset. The narratives were segmented into clauses to quantify clause-initial and usage. Adult judgments of narrative coherence and cohesiveness were elicited as measures of narrative structure. Mean length of utterance (MLU) and clause (MLC) were used as measures of language complexity. Results indicated developmental increases in all measures, but only and-connected dependent clause usage increased with cross-sectional and longitudinal age. Only MLC predicted the relative frequency of clause-initial and regardless of children's age. These results suggest children's frequent use of and to connect events reflects immature language; its association with flat narrative structure is likely epiphenomenal.