Miracle-dz/UniFAR-FTUs huggingface dataset:
DEVELOPMENTAL EXPRESSION OF A TRANSFERRIN IN THE RED IMPORTED FIRE ANT, SOLENOPSIS INVICTA

Transferrin is an iron-binding protein consid- ered to be a component of the innate insect im-mune response (Nichol et al. 2002). Although di- rect causal evidence is lacking, transferrin is thought to sequester the essential nutrient, iron, from invading pathogens (Yoshiga et al. 1997). In-terestingly, a number of insect transferrins have been reported to be up-regulated in response to microbial challenge (Yoshiga et al. 1997, 1999; Yun et al. 1999; Kucharski & Maleszka 2003; Thomp-son et al. 2003; Ampasala et al. 2004; Valles & Pereira 2005). We recently reported the cDNA sequence, gene architecture, and induction of a transferrin from the ﬁre ant, Solenopsis invicta Buren. Because transferrin has been shown to be up-regulated in response to pathogen attack, we were interested to know if the expression of S. in- victa transferrin (SiTf) was developmentally regulated. In addition, we evaluated the effects of the insect hormones, juvenile hormone and 20-hydrox-yecdysone, on SiTrf expression. Expression of the SiTrf gene was quantiﬁed with real-time quantitative PCR (Q-PCR) by the comparative C (Livak (Valles
A versatile targeting system with lentiviral vectors bearing the biotin‐adaptor peptide

Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin.
Mental health needs in adolescents with intellectual disabilities: cross-sectional survey of a service sample.

BACKGROUND Little research has been conducted on the mental health needs of adolescents with intellectual disability, despite the severity and rates of such needs being high throughout childhood and in adulthood. We have investigated the prevalence and predictors of mental health needs and service use in adolescents with intellectual disabilities. MATERIALS AND METHODS Service-based sample (n = 75) in one catchment area. Individual assessments were carried out. The main outcome was the presence of mental health needs measured by the Developmental Behaviour Checklist. RESULTS Prevalence of mental health needs increased from 51% as reported by parents to 67% as judged by clinical interviews. Caseness was associated with low adaptive functioning, diagnosis of autism and family history of mental illness. High scores on parent reports of participant mental ill-health showed negative correlations with adaptive functioning scores. Most individuals were in receipt of social and health care. Half of the participants had sought help for mental health needs. Almost half of those receiving medication were on psychiatric medication. CONCLUSIONS Adolescents with intellectual disabilities may have considerable mental health problems which are functionally impairing yet frequently unidentified and hence untreated. Identification of those at risk and undertaking of a comprehensive needs assessment are essential to maximize potential and quality of life and to reduce further deficits and social exclusion.
A CASE OF KERATIN-POSITIVE ACUTE MYELOID LEUKEMIA: A POSSIBLE ROLE FOR CYTOKERATIN 19 AS A SPECIFIC EPITHELIAL MARKER

Summary A patient with relapsed acute myelomonocytic leukemia (AML, FAB M4) developed skin infiltration by leukemic blasts. On immunochemistry, the blasts showed "dot" positive cytoplasmic staining for cytokeratins AE1/AE3 and CAM 5.2, resembling the pattern seen in Merkel cell carcinoma of skin. However, the blasts were positive for myeloid markers and negative for cytokeratin 19 and chromogranin. Aberrant immunochemical staining can lead to misdiagnosis unless a panel of antibodies of known specificity is used in tumor diagnosis, and the clinical context is taken into account. The possible role of cytokeratin 19 as a more specific marker for epithelia than keratin cocktails is discussed.
Maternal cypermethrin exposure during lactation impairs testicular development and spermatogenesis in male mouse offspring

Within the last decade, numerous epidemiological studies have demonstrated that endocrine disruptors are a possible cause for a decline in semen quality. Cypermethrin is a widely used pyrethroid insecticide, but little is known about its potentially adverse effects on male reproduction. In the present study, we investigated the effects of maternal cypermethrin exposure during lactation on testicular development and spermatogenesis in male offspring. Maternal mice were administered with cypermethrin (25 mg/kg) by gavage daily from postnatal day 0 (PND0) to PND21. Results showed that the weight of testes at PND21 was significantly decreased in pups whose mothers were exposed to cypermethrin during lactation. Maternal cypermethrin exposure during lactation markedly decreased the layers of spermatogenic cells, increased the inside diameter of seminiferous tubules, and disturbed the array of spermatogenic cells in testes of pups at PND21. In addition, maternal cypermethrin exposure during lactation markedly reduced mRNA and protein levels of testicular P450scc, a testosterone (T) synthetic enzyme. Correspondingly, the level of serum and testicular T at weaning was significantly decreased in pups whose mothers were exposed to cypermethrin during lactation. Although the expression of testicular T synthetic enzymes and serum and testicular T in adulthood had restored to control level, the decreased testicular weight and histological changes were irreversible. Importantly, the number of spermatozoa was significantly decreased in adult male offspring whose mothers were exposed to cypermethrin during lactation. In conclusion, maternal cypermethrin exposure during lactation permanently impairs testicular development and spermatogenesis in male offspring, whereas cypermethrin‐induced endocrine disruption is reversible. © 2010 Wiley Periodicals, Inc. Environ Toxicol 2011.
A SURVEY ON EARLY DETECTION AND PREDICTION OF LUNG CANCER

Lung cancer is the leading cause of cancer death worldwide. The earlier detection of lung cancer is a challenging problem due to structure of cancer cell, where most of the cells are overlapped each other. For early detection and treatment stages image processing technique are widely used and for prediction of lung cancer, identification of genetic as well as environmental factors are very important in developing novel method of lung cancer prevention. In various cancer tumours such as lung cancer the time factor is very important to discover the abnormality issue in target images. Prediction of lung cancer we consider significant pattern and their corresponding weight age and score using decision tree algorithm. Using the significant pattern tool for lung cancer prediction system will develop. In this proposed system we use Histogram Equalization is used for preprocessing of images and feature extraction processes and neural network classifier to check the state of patient whether it is normal or abnormal. If the lung cancer is successfully detected and predicted in its early stages will reduce many treatment options and also reduce risk of invasive surgery and increase survival rate. Therefore lung cancer detection and prediction system will propose which is easy, cost effective and time saving. This will produce promising result for detection and prediction of lung cancer. Therefore early detection and prediction of lung cancer should play a vital role in the diagnosis process and also increase the survival rate of patient.
Cytotoxicity of T-2 toxin and its metabolites determined with the neutral red cell viability assay

The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2, the sequence of potency for a series of mycotoxins was T-2 greater than HT-2 greater than T-2 triol greater than T-2 tetraol.
Uncertainty-Based Design of a Bilayer Distribution System for Improved Integration of PHEVs and PV Arrays

Recent years have seen increased interest in green technologies such as photovoltaic (PV) electricity and plug-in hybrid electric vehicles (PHEVs). Such technologies, however, have been found to be detrimental to distribution networks. This paper introduces a novel distribution system architecture that can better accommodate the expected growth in PV electricity and PHEVs. In the proposed architecture, the distribution system becomes a bilayer system composed of the traditional ac layer that serves existing system loads, plus an embedded dc layer that interfaces with PV arrays and PHEVs. A bidirectional converter interconnects the two layers and controls the power flows between them. This paper presents the key design and operational aspects of the proposed architecture, with consideration of different uncertainties inherent in the system. To this end, a probabilistic benchmark has been developed for modeling these uncertainties and for use with the sizing and scheduling of different system components. Monte Carlo (MC) simulations confirmed the technical and economic merit of the proposed design methodology.
Statistical issues in the assessment of the evidence for an interaction between factors in epilepsy trials

We examine the common clinical belief that there is an interaction between epilepsy type and the two standard anti‐epileptic drugs, valproate and carbamazepine, using data from several randomized clinical trials. Epilepsy type is not always easy to define, and three possible reclassifications are investigated to see whether misclassification of epilepsy type within the trials has potentially masked such an interaction. Regression modelling is employed to investigate whether heterogeneity between trial results can be explained by patient factors. Our work suggests that uncertainty in epilepsy type classification should be recognized in future studies. We also generate the hypothesis that the interaction of drug effect with age may reflect the perceived interaction with epilepsy type. We suggest that in any context where misclassification is likely, it is worth considering the use of an explicit ‘unclassified’ group, and investigating whether additional covariates can answer the substantive question. Copyright © 2002 John Wiley & Sons, Ltd.
Refining psychiatric phenotypes for response to treatment: Contribution of LPHN3 in ADHD

Attention deficit/hyperactivity disorder (ADHD) is a heterogeneous disorder characterized by inappropriate levels of attention, hyperactivity, and impulsivity. Although a strong genetic component to the disorder has been established, the molecular genetic underpinnings of this disorder remain elusive. Recently, several studies have reported an association between polymorphisms within the latrophilin 3 gene (LPHN3) and ADHD. Interestingly, the same single‐nucleotide polymorphism conferring susceptibility to ADHD has also been found to predict efficacy of stimulant medication in children. The main objectives of the current article are: (i) To tackle the phenotype heterogeneity issue in ADHD by defining an objective and quantitative measure of response to treatment in a sample of ADHD children based on a hand held automatic device (Actiwatch) and (ii) to use this measure to reproduce for the first time the association between LPHN3 variants and response to methylphenidate (MPH) using a double‐blind, placebo‐controlled crossover experimental design. The results of our study confirm the hypothesis that LPHN3 is associated with response to MPH in ADHD children. Although this will require further validation, our work suggests that the use of an objective measure of response to treatment, such as the change in the child's motor activity measured by Actiwatch, has the potential to uncover genetic association signals that in some conditions might not be obtained using more subjective measures, such as the clinical consensus rating, for example. © 2012 Wiley Periodicals, Inc.
Histogram of oriented gradient and multi layer feed forward neural network for facial expression identification

In this paper an automatic system of performance review has been proposed for facial expression recognition issue. The system is essentially based on histograms of oriented gradient features and multi layer feed forward neural network. The performance was evaluated on three different dataset by varying two parameters: face resolution and color space of images. Results demonstrate that good recognition rates can be obtained using small image resolution in both color spaces grayscale and RGB.
Screening for Escherichia coli O157:H7--a nationwide survey of clinical laboratories

The Association of State and Territorial Public Health Laboratory Directors has recommended that clinical laboratories screen at least all bloody stools for Escherichia coli O157:H7. We contacted the microbiology supervisors of 230 randomly selected clinical laboratories in the United States and administered a standardized questionnaire regarding stool culture practices. Of the 129 laboratories that performed stool cultures, only 70 (54%) reported screening either all stools or all bloody stools submitted for culture for E. coli O157:H7.
Structural characterization of Staphylococcus aureus biotin protein ligase and interaction partners: An antibiotic target

The essential metabolic enzyme biotin protein ligase (BPL) is a potential target for the development of new antibiotics required to combat drug‐resistant pathogens. Staphylococcus aureus BPL (SaBPL) is a bifunctional protein, possessing both biotin ligase and transcription repressor activities. This positions BPL as a key regulator of several important metabolic pathways. Here, we report the structural analysis of both holo‐ and apo‐forms of SaBPL using X‐ray crystallography. We also present small‐angle X‐ray scattering data of SaBPL in complex with its biotin‐carboxyl carrier protein substrate as well as the SaBPL:DNA complex that underlies repression. This has revealed the molecular basis of ligand (biotinyl‐5′‐AMP) binding and conformational changes associated with catalysis and repressor function. These data provide new information to better understand the bifunctional activities of SaBPL and to inform future strategies for antibiotic discovery.
Sparse Discriminant Analysis Line Clemmensen

Classi cation in high-dimensional feature spaces where interpretation and dimension reduction are of great importance is common in biological and medical applications. For these applications standard methods such as microarrays, 1D NMR, and spectroscopy have become everyday tools for measuring thousands of features in samples of interest. The samples are often costly and therefore many problems have few observations in relation to the number of features to be measured. Traditionally data are analyzed by rst performing a feature selection before classi cation. We propose a method which performs
(S)CRUD pattern support for semantic web applications

The use of ontologies (and ontological representations such as OWL) has an increasing tendency in web-based applications development that employ semantics for automatic information processing. One of the most important operations in software applications are the those related with persistent store of the real world entities in repositories, commonly known as CRUD operations (Create-Retrieve-UpdateDelete). Most of the applications are in present developed using objectoriented paradigm. In applications taking the advantage of the Semantic Web technologies, the CRUD operations are performed over ontological repositories extended with search operation. In this paper we present an approach to carry out these operations. It is based on the automatic object ontology mapping with bean generation and (semi)automatic form generation. Our approach is evaluated in two domains – online labor market and scientific publication within a portal offering information in particular domain.
Practical RSA-PAKE for Low-Power Device in Imbalanced Wireless Networks

For enhancing the security of ubiquitous communication, we have to consider three keywords: mobility, wireless, and low computing capability. In this paper, we study one of suitable security protocols for the ubiquitous communication environment. We discuss RSA-based password-authenticated key exchange (RSA-PAKE) protocols for imbalanced wireless networks where a party uses a low-power device to communicate with another party equipped with a powerful computing device. For imbalanced wireless network applications, it is important to reduce the cost of communication for a low-power device even though the cost for powerful devices is increasing. The most power-consuming operation in RSA-PAKE protocols is the reliability test of unauthorized RSA public keys. Hence, it is important to design an efficient reliability test method to construct an efficient RSA-PAKE protocol. In this paper, we propose a new reliability test technique and design a provably secure RSA-PAKE protocol using the technique. Our protocol is suitable for securing the communications conducted over imbalanced wireless networks since the operations computed by one communicating party are efficient enough to be implemented on most low-power devices such as mobile phones and PDAs. The cost of a low-power device is reduced by 84.25% compared with CEKEP, the most efficient RSA-PAKE protocol. We prove the security of our protocol under a firmly formalized security model.
PARAMETERIZING CONJUGACY CLASSES OF MAXIMAL UNRAMIFIED TORI VIA BRUHAT-TITS THEORY

Let denotea field with discretevaluation. We assumethat is completewith perfectresiduefield . Let denotethegroupof -rationalpointsof a reducti ve,linearalgebraic group definedover . A torus in is said to be unramifiedif it splits over an unramified extensionof . Let denotethesetof conjugacy classesof maximalunramifiedtori in . Let denotethesetof pairs where is a facetin theBruhat-Tits building of and is amaximal -anisotropictorusin (theconnectedreducti ve -groupassociatedto ). Thereis anatural equivalencerelation,to bedenoted , on . We show that thereis a bijective correspondence
Single Particle Trajectories in a Nonadiabatic Cusp Magnetic Field Configuration

Charged particle trajectories are numerically analyzed in a cusp magnetic field. One of the significant observations is that the initial phase of the particle is a critical parameter controlling the conversion of the parallel component of velocity to the perpendicular component. This conversion is also studied for different magnetic field gradient scale lengths in the cusp field region.
Recent advances in reconfigurable microwave filters

In today´s communication systems the demand for tunable filters with high performance and with independent control of the filter parameters have been growing. In this paper an overview of several tunable filters that have been proposed over the last years is presented, focused on recent developments involving independent frequency, bandwidth and selectivity control, and high resonator Q filters.
Mechanistic and signaling analysis of Muc4–ErbB2 signaling module: New insights into the mechanism of ligand‐independent ErbB2 activity

The membrane mucin Muc4 is aberrantly expressed in numerous epithelial carcinomas and is currently used as a cancer diagnostic and prognostic tool. Muc4 can also potentiate signal transduction by modulating differential ErbB2 phosphorylation in the absence and in the presence of the ErbB3 soluble ligand heregulin (HRG‐β1). These features of Muc4 suggest that Muc4 is not merely a cancer marker, but an oncogenic factor with a unique‐binding/activation relationship with the receptor ErbB2. In the present study, we examined the signaling mechanisms that are associated with the Muc4–ErbB2 module by analyzing ErbB2 differential signaling in response to Muc4 expression. Our study was carried out in the A375 human melanoma and BT‐474 breast cancer cell lines as our model systems. Quantitative and comparative signaling modulations were evaluated by immunoblot using phospho‐specific antibodies, and densitometry analysis. Signaling complex components were identified by chemical cross‐linking, fractionation by gel filtration, immunoprecipitation, and immunoblotting. Activated downstream signaling pathways were analyzed by an antibody microarray screen and immunoblot analyses. Our results indicate that Muc4 modulates ErbB2 signaling potential significantly by stabilizing and directly interacting with the ErbB2–ErbB3 heterodimer. Further analyses indicate that Muc4 promotes ErbB2 autocatalysis, but it has no effect on ErbB3 phosphorylation, although the chemical cross‐linking data indicated that the signaling module is composed of Muc4, ErbB2, and ErbB3. Our microarray analysis indicates that Muc4 expression promotes cell migration by increasing the phosphorylation of the focal adhesion kinase and also through an increase in the levels of β‐catenin. J. Cell. Physiol. 224: 649–657, 2010. © 2010 Wiley‐Liss, Inc.
Static transmission expansion planning using Heuristic and metaheuristic techniques

This paper describes a hybrid tool to perform Static Transmission Expansion Planning, STEP, studies and its application to the Garver6-Bus academic system and to the Southern Brazilian Transmission equivalent real system. The developed STEP tool integrates two phases as follows. The first one uses Constructive Heuristic Algorithms (CHA) to reduce the search space, and the second uses Particle Swarm Optimization (PSO) to identify the final solution. This hybridization between CHAs and PSO proved to be very effective and shows good performance to reduce the size of the STEP search space and to identify good quality solutions. These are relevant issues given the combinatorial nature of investment problems leading to the explosion of the number of alternative plans, one of the greatest difficulties faced in this planning problem.
An OTDR event analysis algorithm based on EMD-based denoising and wavelet transform

In order to improve the events analysis performance of optical time domain reflectometer (OTDR), locate the events accuracy and speedy, a new event analysis algorithm is particularly needed. Since OTDR signal contains nonstationary noise and the events can be seen as singularities, we presented an algorithm based on Empirical Mode Decomposition (EMD) and wavelet transform. Firstly using EMD to denoise the signal and then detecting the singularities by wavelet transform. This algorithm combines the advantage of EMD and wavelet transform. Experimental results show that this algorithm can detect and locate OTDR events effectively, and has great application value.