Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

Secreted proteins, which include cytokines, hormones and growth factors, are extracellular ligands that control key signaling pathways mediating cell-cell communication within and between tissues and organs. Many drugs target secreted ligands and their cell-surface receptors. Still, there are hundreds of secreted human proteins that either have no identified receptors (“orphans”) and are likely to act through cell surface receptors that have not yet been characterized. Discovery of secreted ligand-receptor interactions by high-throughput screening has been problematic, because the most commonly used high-throughput methods for protein-protein interaction (PPI) screening do not work well for extracellular interactions. Cell-based screening is a promising technology for definition of new ligand-receptor interactions, because multimerized ligands can enrich for cells expressing low affinity cell-surface receptors, and such methods do not require purification of receptor extracellular domains. Here, we present a proteo-genomic cell-based CRISPR activation (CRISPRa) enrichment screening platform employing customized pooled cell surface receptor sgRNA libraries in combination with a magnetic bead selection-based enrichment workflow for rapid, parallel ligand-receptor deorphanization. We curated 80 potentially high value orphan secreted proteins and ultimately screened 20 secreted ligands against two cell sgRNA libraries with targeted expression of all single-pass (TM1) or multi-pass (TM2+) receptors by CRISPRa. We identified previously unknown interactions in 12 of these screens, and validated several of them using surface plasmon resonance and/or cell binding. The newly deorphanized ligands include three receptor tyrosine phosphatase (RPTP) ligands and a chemokine like protein that binds to killer cell inhibitory receptors (KIR’s). These new interactions provide a resource for future investigations of interactions between the human secreted and membrane proteomes.
Cognitive Computing: Architecture, Technologies and Intelligent Applications

With the development of network-enabled sensors and artificial intelligence algorithms, various human-centered smart systems are proposed to provide services with higher quality, such as smart healthcare, affective interaction, and autonomous driving. Considering cognitive computing is an indispensable technology to develop these smart systems, this paper proposes human-centered computing assisted by cognitive computing and cloud computing. First, we provide a comprehensive investigation of cognitive computing, including its evolution from knowledge discovery, cognitive science, and big data. Then, the system architecture of cognitive computing is proposed, which consists of three critical technologies, i.e., networking (e.g., Internet of Things), analytics (e.g., reinforcement learning and deep learning), and cloud computing. Finally, it describes the representative applications of human-centered cognitive computing, including robot technology, emotional communication system, and medical cognitive system.
Advanced Industrial Test-Retest Reliability of Kinect’s Torso Joint Prediction for Compensatory Assessment After Stroke

Stroke patients typically incorporate compensatory strategies to complement the minimal use of distal segment of the upper limb to perform daily activities. The ability to monitor compensatory strategies in terms of abnormal torso and shoulder movements through Kinect is attractive due to its portability and cost. However, Kinect’s joint proposals can be undermined with the occluded pose typical of stroke patients. This study investigated these discrepancies through test-retest procedures of static pose typical to stroke patients. It was found that at the distance of approximately 2.5 meters, all the joint proposals were reliable for normal pose. However, only joints attributed to the torso were reliable when stroke pose is prevalent. Therefore, joints attributed to torso can be readily measured from the intrinsic biomechanical model but model fidelity of distal segments must be improved for useful assessment.
MORPHOLOGICAL AND TECHNOLOGICAL STUDIES ON SOME CANOLA ( Brassica napus, L .) GENOTYPES

Morphological and technological traits were utilized to identify among canola ( Brassica napus, L .) genotypes namely Pactol, Serw 6, Serw 10 and Serw 4. Two field experiments were carried out at the Farm of El-Serw Agricultural Research Station during 2006/2007and 2007/2008 seasons. The results revealed that plants of Line Pactol can be identified by leaf length (blade and petiole), leaf petiole length, plant height (171.1 cm), oil content (44.7%) and branches point (60cm) and its lowest Line in cotyledon length and width, number of siliqua/ plant (286,8) number of seed/ siliqua (22) and seed yield/plant (32.1 g). Plants of Line Serw 6 can be identified with higher number of leaf dentation, plant height (175.9 cm), 1000-seed weight (3.40 g), lowest means in number of leaf lobes (4.5), petiole florescence length (39.9 cm), No. of side branches (6.5) and oil content (40.2%). Plants of Line Serw 10 can be identified with its leaf width (10.1 cm), length of flower petals (1.5 cm), branches point (11.5 cm), lowest means in leaf length, no. of leaf dents (19), length of leaf petiole (7.1 cm) and number of leaves/ plant. Plants of cultivar Serw 4 can be identified by higher number of leaf lobes (5.6), number of leaves (10), petiole florescence length (47.9 cm), number of side branches (13), length of beak (1.2 cm), number of siliqua/ plant (888.1), number of seed/ siliqua (30), seed yield/plant (68.6gm), lowest means in leaf length (21.4 cm), width (7.9 cm), branches point (3.4 cm) and 1000-seed weight (2.77 g). Dice similarity coefficients between genotypes was calculated. Maximum 87% and minimum 62% similarity coefficients were observed between Line Pactol and variety Serw 4, Line Pactol and Line Serw 10, respectively. Base on the cluster analysis, genotypes were grouped into two clusters. Line Serw 10 was in a separate cluster while, Line Pactol, Line Serw 6 and variety Serw 4 were in the second cluster. The results confirmed that some morphological traits and RAPD method could be used for identify among canola genotypes.
ULTRASONOGRAPHY : Diagnostic Performance of Real-time Shear-Wave Elastography in Prostate Cancer

1 From the Departments of Adult Radiology (J.M.C., A.M.T., A.K.) and Histopathology (V.V., O.H.), Necker University Hospital, Paris, France; Department of Urology, Hôpital Européen Georges Pompidou, Paris, France (A.M.); Department of Urology, Northeastern Ohio Medical University, Youngstown, Ohio (R.M.); and Radiology Consultants, 250 DeBartolo Pl, Youngstown, OH 44512 (R.G.B.). Received March 5, 2014; revision requested April 24; revision received July 25; accepted August 25; final version accepted August 28. Supported by SuperSonic Imagine, Aix-en-Provence, France. Address correspondence to R.G. B. (e-mail: rgbarr@zoominternet.net).
AN ILLUSTRATIVE RESEARCH TO ASSESS “NDRD” (NON-DIABETIC RENAL DISEASE) FREQUENCY IN THE PATIENTS DIAGNOSED WITH T2DM (TYPE 2 DIABETES MELLITUS)

: Objective: In type 2 diabetes patients who encounter renal biopsy for unbalanced kidney functions, to measure the frequency NDRD is the main objective of this research. These unbalanced functions of kidney comprised hematuria, proteinuria in the nephrotic and non-nephrotic range and quality increase kidney failure. Methods: This research held at Allied Hospital, Faisalabad from January 2017 to December 2017 (Department of Pathology). This assessment of kidney surgery was done of 73 patients of type 2 diabetes. These patients were also likely to have kidney disorder except diabetic glomerulosclerosis (DGS). This was due to unidentified hematuria, in nephrotic and non-nephrotic position presence clinically important proteinuria and quickly increasing kidney failure or unidentified kidney failure with usual kidney size. As observed on the light microscope and immunofluorescence investigations, the damaged kidney was reported in three group of patients. The patients in group-1 had just NDRD, in group 2, patients have NDRD along with DGS and patients’ group 3 had only DGS. Using SPSS software, the association of investigation with the detached figure in each group and diabetes timing were measured and estimated. Result: Total 73 patients were included in the study. Out of these 73, males and females were 46 and 27 respectively (1 7:1). During the performance, group 1 and 2 tries to have patients with low ages (49.26 ±9.37 years and 49.0 ±5.27 years respectively) as compared with group 3. (53.62 ±6.62 years). The mean diabetic time period in group 1 was observed 9.9 ±10.42 years in group 2-time duration was 7.5 ±3.38, and in group 3, 13.31 ±1.71. 30 patients (41.1%) out of 73 had only NDRD, 6(8.21%) had NDRD along with DGS while in 37(50.7%), only DGGS was noticed. The abrasions that were very usual include Focal segment glomerulosclerosis (FSGS) and tubulointerstitial nephritis (TIN) and their presence was reported as 22.2% and 19.4% respectively. In NDRD patients of group 1 and 2 (3.064±1.38gms/24hrs and 3.316 ±0.97 gms/24hrs respectively) the quantity of proteinuria was higher, as compared to DGS patients of group 3 (2.815±0.916 gms/24 hrs.). But these values are not important statistically. If we compare serum cretonne in all groups, it was found remarkably higher in group 3 patients (3.391±0.95 mg(di) and group 1 (2.563±0.95 mgdL) and group 2 (2.633±0.952 mg dl) (p=0.0002). In NDRD patients, the hematuria was noticed in higher quantity (32.5% in group 1 and 33.3%) in group 2) as compare to patients of DGS in group 3 But these figures are not imported statistically. Conclusion: The study suggests that in type 2 diabetic patients, a high quantity of NDRD is present. These patients encounter surgery of kidney due to due to the damaged working of the kidney. The study also highlights the importance of surgery in patients experiencing the renal failure so that control disease could be adapted accordingly.
EFFECT OF THE HERBICIDE OXYFLUORFEN ON NITRATE REDUCTASE AND NITRATE CONTENT IN Zea mays L. SEEDLINGS

Oxyfluorfen spraying resulted in distinct lowering of nitrate reductase with both doses of the herbicide (5 ppm and 15 ppm) and in both roots and leaves of maize seedlings throughout the run of the experiments. After 5 days of spraying, diminishment of the enzyme amounted to 37% and 56% with the lowest dose of the herbicide in roots and leaves respectively. A similar pattern was observed after 10 and 15 days of spraying. On the other hand, the nitrate content behaved similarly indicating induction of nitrate reductase by nitrate availability. Lowering of nitrate reductase and nitrate content by oxyfluorfen treatment could be attributed to decline in carbon assimilation by some herbicides as verified by several workers, and nitrate uptake and reduction to nitrate reductase are energy expending processes.
Supporting Collaborative Interaction among Learners Using Collaborative Learning System InCircle

—This paper describes the development and the evaluation of a collaborative communication system called InCircle. One notable trend in the pedagogical field is a shift from a teacher-centered learning to a student-centered one. Recent advancement of Information and Communication Technology (ICT) has accelerated the shift. The spread of various mobile applications for educational use has enabled us to share information and knowledge in real time. In order to promote student-centered learning, it is necessary to facilitate interaction among learners. For these purposes, InCircle was introduced in a university level language and culture class in Japan. The objectives of our research study were to examine whether InCircle contributes to the facilitation of interaction among learners and the enhancement of mutual cross-cultural understanding. The evaluation was conducted in an international exchange subject where international students and Japanese students learn about the language and culture at university in Japan. The result of the questionnaire showed the learners' preference to InCircle as opposed to the blog comment function. It also endorsed its usability and effectiveness as a communication tool.
High-CO 2 /hypoxia-responsive transcription factors DkERF24 and 12 DkWRKY1 interact and activate DkPDC2 promoter

57 58 transcription factors is important for understanding plant responses to natural anaerobic 59 environments and during storage and transport of fresh horticultural products. In this 60 study, yeast one-hybrid (Y1H) library screening using the persimmon ( Diospyros kaki ) 61 pyruvate decarboxylase ( DkPDC2 ) promoter identified three Ethylene Response 62 Factor genes ( DkERF23/24/25 ) and four WRKY transcription factor genes 63 ( DkWRKY1/5/6/7 ) that were differentially expressed in response to high CO 2 (95%, 64 with 4% N 2 and 1% O 2 ) and high N 2 (99% N 2 and 1% O 2 ). Y1H assays and 65 electrophoretic mobility shift assays indicated that DkERF23/24/25 and DkWRKY6/7 66 could directly bind to the DkPDC2 promoter. Dual-luciferase assays confirmed that 67 these transcription factors were capable of transactivating the DkPDC2 promoter. in combination synergistically transactivated the DkPDC2 promoter, and yeast two-hybrid and bimolecular fluorescence complementation assays confirmed protein–protein interaction between DkERF24 and DkWRKY1. Transient over-expression of DkERF24 and DkWRKY1 separately and in combination in persimmon fruit discs were effective in maintaining insolubilization of tannins, concomitantly with the accumulation of DkPDC2 transcripts. Studies with Arabidopsis thaliana homologs AtERF1 and AtWRKY53 indicated that similar protein–protein interactions and synergistic regulatory effects also occur with the DkPDC2 promoter. We propose that an ERF and WRKY transcription factor complex contributes to responses to hypoxia in both persimmon fruit and Arabidopsis, and the possibility that this is a general plant response requires further investigation.
Designing Effective Advergame for Purchase Intention: A View of Game Design and Psychological State

-Advergame has increasingly been used for a new marketing campaign. This study defines a new research model with a relationship structure of advergame design and individual psychological state as antecedents for advergame attitude, brand attitude, and purchase intention, based on the hierarchy of effects theory. An empirical study for online survey collects 522 consumers who have recent experience of advergame. The results found that game-brand fit, perceived interactivity, and involvement are the key drivers of advergame attitude. Further, advergame attitude and brand attitude were found to be two important mediators of purchase intention. Implications for marketers and scholars are further discussed.
Sign Language Recognition using Neural Networks

– Sign language plays a great role as communication media for people with hearing difficulties.In developed countries, systems are made for overcoming a problem in communication with deaf people. This encouraged us to develop a system for the Bosnian sign language since there is a need for such system. The work is done with the use of digital image processing methods providing a system that teaches a multilayer neural network using a back propagation algorithm. Images are processed by feature extraction methods, and by masking method the data set has been created. Training is done using cross validation method for better performance thus; an accuracy of 84% is achieved .
Relationship between Polynomial Geometric Surfaces Terms and Observation Points Numbers and Effect in the Accuracy of Geometric Geoid Models

— The application of the geometric method of local geoid model determination which requires the fitting of geometric surfaces to known geoid heights to enable geoid heights of new points to be interpolated involves the use of least squares technique for computation of the models' parameters. The selection of polynomial geometric surfaces depends on the size of the study area, the variation of the geoid heights and the number of measurement points. The accuracy of the geometric geoid model increases as the number of observation points approximates the number of geometric surface terms. But in most cases, the number of observation points is not considered. To this effect, this paper presents the relationship between geometric surfaces terms and observation points numbers and effect in the accuracy of geometric geoid models. A total of 23 points of known local gravimetric geoid heights were used. Two polynomial geometric (third and fifth degrees) surfaces were fitted to the geoid heights at various observation point numbers and compared to determine the relationship between the number of model terms and that of observation points and effect in the accuracy of the models. Least squares adjustment technique was applied to obtain the model parameters. The differences between the models and the known geoid heights of the points were computed and used to obtain the RMSEs as well as the accuracy of the models. The obtained results showed that the accuracy of the polynomial geometric geoid models tends to the highest as the number of measurement points approximates the number of the model terms and in a unique solution where the number of observation points is equal to the number of the polynomial geometric model terms, the model accuracy is highest. The paper recommends that the geometric method of local geoid model determination should be strictly applied in small areas. Where the method will be applied in considerable large areas, higher degrees polynomial geometric surfaces with a larger number of terms approximating the number of observation points should be applied. This will enable a proper fit of the polynomial surface to the known geoid heights, as well as high accuracy to be obtained.
Intrahepatic triglyceride content: influence of metabolic and genetics drivers

Purpose of review Intrahepatic triglyceride (IHTG) content is determined by substrate flux to, fatty acid synthesis and partitioning within, and triglyceride disposal from the liver. Dysregulation of these processes may cause IHTG accumulation, potentially leading to nonalcoholic fatty liver disease. The aetiology of IHTG accumulation has not been fully elucidated; however, environmental factors and heritability are important. Here, we review recent evidence regarding the contribution of metabolic and genetic components of IHTG accumulation. Recent findings Obesity and insulin resistance are the primary metabolic drivers for IHTG accumulation. These risk factors have pronounced and seemingly overlapping effects on all processes involved in determining IHTG content. The strong and interchangeable associations between obesity, insulin resistance and IHTG make it challenging to determine their relative contributions. Genome-wide association studies have identified a growing list of single nucleotide polymorphisms associated with IHTG content and recent work has begun to elucidate their mechanistic effects. The mechanisms underlying metabolic and genetic drivers of IHTG appear to be distinct. Summary Both metabolic and genetic factors influence IHTG content by apparently distinct mechanisms. Further work is needed to determine metabolic and genetic interaction effects, which may lead to more personalized and potentially efficacious therapeutic interventions. The development of a comprehensive polygenic risk score for IHTG content may help facilitate this.
PUMA-560 Robot Manipulator Position Sliding Mode Control Methods Using MATLAB/SIMULINK and Their Integration into Graduate/Undergraduate Nonlinear Control, Robotics and MATLAB Courses

This paper describes the MATLAB/SIMULINK realization of the PUMA 560 robot manipulator position control methodology. This paper focuses on two main areas, namely robot manipulator analysis and implementation, and design, analyzed and implement nonlinear sliding mode control (SMC) methods. These simulation models are developed as a part of a software laboratory to support and enhance graduate/undergraduate robotics courses, nonlinear control courses and MATLAB/SIMULINK courses at research and development company (SSP Co.) research center, Shiraz, Iran.
Nonsteroidal anti-inflammatory drugs and cardiovascular disease risk in spondyloarthritis-spectrum diseases

Purpose of review Increased cardiovascular (CV) risk associated with nonsteroidal anti-inflammatory drugs (NSAIDs) is well recognized in the general population. This may limit the use of this effective therapy in patients with spondyloarthritis (SpA), a population already at high CV risk. Recent findings Increased CV diseases and their risk factors in patients with SpA were consistently shown in recent population-level data. NSAIDs remained commonly prescribed in SpA, though their structural benefit remained controversial and the dispensing practice was variable in different regions in the world. A previous observation study suggested NSAIDs in SpA might be cardio-protective, possibly via their modulation of the chronic inflammatory state. A recent meta-analysis of nonrandomized studies also revealed no increased risk of a CV event. Interestingly, there is growing evidence that different NSAIDs might impose differential CV risk on patients with SpA. Summary Recent evidence suggested NSAIDs were associated with a neutral and possibly lower CV risk in patients with SpA, which provided some reassurance for their use.
Influence of Photon Pump Fluence on Charge Carriers in FAPbI 3 and Manganite Perovskites

FAPbI 3 and FA(Mn:Pb)I 3 perovskite films were prepared and evaluated through steady and transient absorption spectroscopy. According to the analysis using Elliot’s model, there were no considerable differences except for the absorption intensity between FAPbI 3 and FA(Mn:Pb)I 3 perovskite films: the value of the optical gap (E g ) and the position of exciton resonance (E 0 ) were the same. The femtosecond transient absorption showed biexponential relaxation properties of the charge carriers, suggesting that biexcitons are more easily generated in FA(Mn:Pb)I 3 than FAPbI 3 perovskite. The generation of biexcitons in FA(Mn:Pb)I 3 was also confirmed by the photon pump fluence dependence. Moreover, we were able to estimate the average number of absorbed photons <N> directly from the photon pump power dependence without needing any further experimental measurements such as photoluminescence. Our findings may offer a new way of understanding photoinduced carrier dynamics in perovskite manganites.
Magnetic force induced tristability for dielectric elastomer actuators

This paper presents a novel dielectric elastomer actuator (DEA) with three stable states. By introducing magnetic forces and coupling them with two cone dielectric elastomer (DE) films, an inherent tristability for the DEA is obtained with a compact design. It is easy to switch between the three stable states by controlling the voltages applied to the DE films. A theoretical model of the system’s potential energy that contains the free energy of the DEs and the potential energy of the applied magnetic field was developed for the tristable mechanism. The experimental results demonstrate that controllable transitions between the three stable states can be achieved with this design by applying over-critical voltages to the various DE films. The maximum dynamic range of the DEA can exceed 53.8% of the total length of the device and the DE’s creep speed was accelerated under the action of the magnetic field.