Miracle-dz/UniFAR-FTUs huggingface dataset:
How pharmacoepidemiology networks can manage distributed analyses to improve replicability and transparency and minimize bias

Several pharmacoepidemiology networks have been developed over the past decade that use a distributed approach, implementing the same analysis at multiple data sites, to preserve privacy and minimize data sharing. Distributed networks are efficient, by interrogating data on very large populations. The structure of these networks can also be leveraged to improve replicability, increase transparency, and reduce bias. We describe some features of distributed networks using, as examples, the Canadian Network for Observational Drug Effect Studies, the Sentinel System in the USA, and the European Research Network of Pharmacovigilance and Pharmacoepidemiology. Common protocols, analysis plans, and data models, with policies on amendments and protocol violations, are key features. These tools ensure that studies can be audited and repeated as necessary. Blinding and strict conflict of interest policies reduce the potential for bias in analyses and interpretation. These developments should improve the timeliness and accuracy of information used to support both clinical and regulatory decisions.
Comorbidity and healthcare use for individuals with CVD in the Ireland: a cross-sectional, population-based study

Objective This study explores the factors associated with health service use for individuals with cardiovascular disease (CVD) and comorbidity in the Ireland. Design Population-based cross-sectional survey. Setting Nationally representative health and health service use survey from the 2010 Quarterly National Household Survey was analysed. Primary outcome measures Four outcome variables were examined: no CVD, CVD only, CVD with CVD-related comorbidities and CVD with non-CVD-related comorbidity. Results Of the 791 individuals reporting doctor-diagnosed CVD, 77% had a second morbidity. Using type of healthcare coverage as a proxy for socioeconomic status, both CVD-related and non CVD-related comorbidity increases the use of health service usage substantially for individuals with CVD, particularly general practitioner services (8.47, CI 4.49 to 15.96 and 5.20, CI 2.10 to 12.84) and inpatient public hospital care (3.64, CI 2.93 to 4.51 and 3.00, CI 2.11 to 4.26). Conclusion This study indicated that even when demographic and socioeconomic factors are controlled for, comorbidity significantly increases the risk of accessing health services for individuals with CVD.
A Study of the Relation Between Mastectomy Specimen Weight and Volume With Implant Size in Oncoplastic Reconstruction

Background: There is no general consensus regarding the best and most accurate method for assessing the size of implant required for achieving the best symmetry in oncoplastic reconstruction. Materials and Methods: Breast weight, volume and size of implant were recorded prospectively on patients undergoing immediate reconstruction over a 3-year period. Cases with specimen size greater than 2,000 g were excluded. The primary endpoint was size of implant used in relation to specimen weight and volume. Secondary endpoints were the influence of age and histology on specimen weight, volume and breast density. Results: A total of 278 mastectomies were performed. Correlation of implant size with volume was marginally stronger than that with weight (r=81%, p<0.001 vs. r=78.9%, p<0.001 respectively). Women aged 50 years and above had lower breast weight for the same breast volume than women under 50 years. The difference was merely 4%, but was statistically significant (p=0.001). Histology had no statistically significant influence on breast density. Conclusion: Specimen weight and volume were found to be closely correlated. Weight can be measured more accurately and easily with reproducible readings compared to volume.
A Wireless Radio Frequency Triggered Acquisition Device (WRAD) for Self-Synchronised Measurements of the Rate of Change of the MRI Gradient Vector Field for Motion Tracking

In this paper, we present a device that is capable of wireless synchronization to the MRI pulse sequence time frame with sub-microsecond precision. This is achieved by detecting radio frequency pulses in the parent pulse sequence using a small resonant circuit. The device incorporates a 3-axis pickup coil, constructed using conventional printed circuit board (PCB) manufacturing techniques, to measure the rate of change of the gradient waveforms with respect to time. Using Maxwell’s equations, assuming negligible rates of change of curl and divergence, a model of the expected gradient derivative (slew) vector field is presented. A 3-axis Hall effect magnetometer allows for the measurement of the direction of the static magnetic field in the device co-ordinate frame. By combining the magnetometer measurement with the pickup coil voltages and slew vector field model, the orientation and position can be determined to within a precision of 0.1 degrees and 0.1 mm, respectively, using a pulse series lasting $880~\mu \textsf {s}$ . The gradient pulses are designed to be sinusoidal, enabling the detection of a phase shift between the time frame of the pickup coil digitization circuit and the gradient amplifiers. The signal processing is performed by a low power micro-controller on the device and the results are transmitted out of the scanner bore using a low latency 2.4 GHz radio link. The device identified an unexpected 40 kHz oscillation relating to the pulse width modulation frequency of the gradient amplifiers that is predominantly in the direction of the static magnetic field. The proposed wireless radio frequency triggered acquisition device enables users to probe the scanner gradient slew vector field with minimal hardware set-up and shows promise for the future developments in the prospective motion correction.
Treatment outcome research of enhanced cognitive behaviour therapy for eating disorders: a systematic review with narrative and meta-analytic synthesis

ABSTRACT Enhanced cognitive behaviour therapy (CBT-E) has recently generated interest as a potentially useful treatment for eating disorders (ED). In the current study, we conducted a systematic review of the outcome literature on CBT-E, with both narrative and meta-analytic synthesis. We included single-group uncontrolled pre-post design studies and randomised control trials (RCTs) of CBT-E, which allowed us to include a larger number of studies, and also compare the two methodologies in terms of effect size. The primary analysis included 15 CBT-E studies with a total of 948 participants. Narrative synthesis examined bingeing and purging episodes, BMI change, and follow-up results. A large, statistically significant effect supported CBT-E as a treatment for all EDs (g = 1.06). When comparing methodologies, both pre-post design studies (g = 1.26) and RCTs (g = 0.82) yielded large effects. Narrative synthesis outlined reductions in ED behaviours and increases in BMI which were maintained at follow-up. Limitations include that further RCTs of CBT-E are needed to establish clinical effectiveness of this treatment approach for all EDs; however, results from narrative exploration indicate this endeavour would be worthwhile. Despite the limitations, this study provides additional support for CBT-E as a successful treatment across the range of EDs.
Embolism resistance in stems of herbaceous Brassicaceae and Asteraceae is linked to differences in woodiness and precipitation

Background and Aims Plant survival under extreme drought events has been associated with xylem vulnerability to embolism (the disruption of water transport due to air bubbles in conduits). Despite the ecological and economic importance of herbaceous species, studies focusing on hydraulic failure in herbs remain scarce. Here, we assess the vulnerability to embolism and anatomical adaptations in stems of seven herbaceous Brassicaceae species occurring in different vegetation zones of the island of Tenerife, Canary Islands, and merged them with a similar hydraulic-anatomical data set for herbaceous Asteraceae from Tenerife. Methods Measurements of vulnerability to xylem embolism using the in situ flow centrifuge technique along with light and transmission electron microscope observations were performed in stems of the herbaceous species. We also assessed the link between embolism resistance vs. mean annual precipitation and anatomical stem characters. Key Results The herbaceous species show a 2-fold variation in stem P50 from -2.1 MPa to -4.9 MPa. Within Hirschfeldia incana and Sisymbrium orientale, there is also a significant stem P50 difference between populations growing in contrasting environments. Variation in stem P50 is mainly explained by mean annual precipitation as well as by the variation in the degree of woodiness (calculated as the proportion of lignified area per total stem area) and to a lesser extent by the thickness of intervessel pit membranes. Moreover, mean annual precipitation explains the total variance in embolism resistance and stem anatomical traits. Conclusions The degree of woodiness and thickness of intervessel pit membranes are good predictors of embolism resistance in the herbaceous Brassicaceae and Asteraceae species studied. Differences in mean annual precipitation across the sampling sites affect embolism resistance and stem anatomical characters, both being important characters determining survival and distribution of the herbaceous eudicots.
A Multimodal Study of Childhood Trauma and Distress Tolerance in Young Adulthood

ABSTRACT Individuals with a history of childhood trauma experience deficits in emotion regulation. However, few studies have investigated childhood trauma and both perceived (i.e., self-report) and behavioral measures of distress tolerance. The current study evaluated associations between childhood trauma (i.e., physical abuse, sexual abuse, and witnessing family violence) and measures of perceived (Distress Tolerance Scale) and behavioral distress tolerance (i.e., Paced Auditory Serial Addition Test, breath-holding). Participants were 320 undergraduate students with a history of interpersonal trauma (e.g., sexual/physical assault). Structural equation modeling was used to evaluate associations between frequency of childhood trauma type and distress tolerance. Greater childhood physical abuse was associated with higher perceived distress tolerance. Greater levels of witnessing family violence were associated with lower behavioral distress tolerance on the breath-holding task. No significant effects were found for Paced Auditory Serial Addition Test performance. Associations between childhood trauma and emotion regulation likely are complex and warrant further study.
Analysis of Golgi pH in Chinese hamster ovary cells using ratiometric pH‐sensitive fluorescent proteins

Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH‐sensitive fluorescent protein (FP), in the cis‐ and trans‐Golgi, were constructed by fusing pHluorin2 with specific targeting proteins, acetylglucosaminyltransferase, and a galactosyltransferase, respectively. Stable CHO cell clones expressing pHluorin2 in the cytoplasm were also constructed. The subcellular localization of FPs was confirmed by immunofluorescence analysis. Live‐cell imaging revealed that the intracellular pH (pHi) of clones expressing the ratiometric pH‐sensitive FPs converged to a specific pH range (cis‐Golgi: 6.4–6.5; trans‐Golgi: 5.9–6.0; and cytoplasm: 7.1–7.2). The pHi was successfully evaluated in various culture conditions. Although culture pH was maintained at 7.2 in a bioreactor, the Golgi pH increased with culture time. Elevated ammonia concentration and osmolality were partially responsible for the increased Golgi pH during bioreactor cultures. Taken together, the application of ratiometric pH‐sensitive FPs in monitoring the Golgi pH of CHO cells during culture provides a new perspective to improve protein glycosylation through pHi control.
Automated Titration of Vasopressor Infusion Using a Closed-loop Controller: In Vivo Feasibility Study Using a Swine Model

What We Already Know about This Topic Intraoperative hypotension has been associated with adverse postoperative outcomes. A randomized controlled trial of individualized blood pressure management in patients undergoing major abdominal surgery found reduced postoperative adverse events in patients in the blood pressure management intervention group versus the standard of care group. What This Article Tells Us That Is New In this study of pigs with normovolemic hypotension induced by administration of sodium nitroprusside, an automated closed-loop vasopressor administration device was able to maintain mean arterial pressure within 5 mmHg of 80 mmHg for 98% of the intraoperative period. This suggests that norepinephrine can be accurately titrated using an automated infusion device in order to maintain target blood pressure. Background: Multiple studies have reported associations between intraoperative hypotension and adverse postoperative complications. One of the most common interventions in the management of hypotension is vasopressor administration. This approach requires careful and frequent vasopressor boluses and/or multiple adjustments of an infusion. The authors recently developed a closed-loop controller that titrates vasopressors to maintain mean arterial pressure (MAP) within set limits. Here, the authors assessed the feasibility and overall performance of this system in a swine model. The authors hypothesized that the closed-loop controller would be able to maintain MAP at a steady, predefined target level of 80 mmHg for greater than 85% of the time. Methods: The authors randomized 14 healthy anesthetized pigs either to a control group or a closed-loop group. Using infusions of sodium nitroprusside at doses between 65 and 130 µg/min, we induced four normovolemic hypotensive challenges of 30 min each. In the control group, nothing was done to correct hypotension. In the closed-loop group, the system automatically titrated norepinephrine doses to achieve a predetermined MAP of 80 mmHg. The primary objective was study time spent within ±5 mmHg of the MAP target. Secondary objectives were performance error, median performance error, median absolute performance error, wobble, and divergence. Results: The controller maintained MAP within ±5 mmHg of the target for 98 ± 1% (mean ± SD) of the time. In the control group, the MAP was 80 ± 5 mmHg for 14.0 ± 2.8% of the time (P< 0.0001). The MAP in the closed-loop group was above the target range for 1.2 ± 1.2% and below it for 0.5 ± 0.9% of the time. Performance error, median performance error, median absolute performance error, wobble, and divergence were all optimal. Conclusions: In this experimental model of induced normovolemic hypotensive episodes in pigs, the automated controller titrated norepinephrine infusion to correct hypotension and keep MAP within ±5 mmHg of target for 98% of management time.
Chronic histological changes in deceased donor kidneys at implantation do not predict graft survival: a single‐centre retrospective analysis

The use of preimplantation kidney biopsies (PIKBs) to aid deceased donor kidney utilization decisions is controversial. Outcomes of transplants that had been biopsied after the decision had been made to implant were analysed, in order to determine the association between chronic histological changes at implantation and graft outcomes. A retrospective analysis of transplants between the year range 2006–2015 was performed. Karpinski scores on biopsies were collected, and graft outcomes were analysed using univariate and multivariable techniques. Also, Karpinski scores from single and dual kidney transplants from older donors were examined to determine if knowledge of the score preoperatively would have altered utilization. Four hundred and eight single kidneys were transplanted. Although kidneys with scores >4 had lower 1‐ and 3‐year median (IQR) estimated glomerular filtration rates (eGFRs) than those scoring 0–4 (51 (37–66) vs. 35 (26–52) ml/min/1.73 m2, P < 0.001, and 52 (34–64) vs. 35 (24–52) ml/min/1.73 m2, P < 0.001, respectively), there was no significant association between Karpinski score and death‐censored graft survival on univariate or multivariable analyses. The utilization analysis (75 single and 25 dual kidney transplant recipients) suggested that systematic use of PIKBs would have resulted in 29% fewer patients being transplanted. This analysis does not support the systematic use of PIKBs to determine deceased donor kidney utilization.
Development and Concurrent Validity of a Composite Social Isolation Index for Older Adults Using the CLSA

RÉSUMÉ L’isolement social a retenu l’attention des chercheurs, en particulier dans la recherche concernant les personnes âgées, étant donné les corrélations établies avec la mortalité et le risque de mauvaise santé physique ou mentale. Toutefois, les mesures utilisées jusqu’à maintenant pour l’isolement social ont grandement varié et manquent souvent de clarté conceptuelle. La présente étude s’appuie sur un cadre conceptuel de classification des mesures d’isolement social et explique l’élaboration et la validation d’un indice composite d’isolement social (ELCV-SII) qui a été créé en utilisant les données de l’Étude longitudinale canadienne sur le vieillissement (ELCV). L’échantillon de l’ELCV incluait 21 491 Canadiens âgés de 65 ans ou plus (âge moyen : 72,9 ans; à 53,5 % de sexe féminin) retrouvés dans les données de base des cohortes de suivi et de la cohorte globale de l’ELCV (n = 51 338). Le CLSA-SII a été élaboré en utilisant une méthodologie standard, qui a été suivie pour la création d’autres mesures, dont la résilience à la multimorbidité et les indices de vieillissement efficaces. Les associations entre l’ELCV-SII et quatre variables clés (échelle de satisfaction de vie, dépression, santé mentale perçue et santé perçue) ont été évaluées par des analyses de régression, en tenant compte de l’âge, du sexe, du revenu et de la scolarité. Les associations entre l’indice CLSA-SII et les quatre variables d’intérêt ont donné lieu à des associations modérées statistiquement significatives conformes à la tendance prévue, qui concordent avec les recherches antérieures, ce qui démontre une bonne validité convergente pour l’ELCV-SII. ABSTRACT Social isolation, given its established association with mortality, and risk of poor physical and/or mental health over the life course, has attracted the attention of researchers. However, such measures have been highly variable and often lack conceptual clarity. This study, which drew on a conceptual framework for classifying social isolation measures, provides a rationale for the development and validation of a composite Social Isolation Index (SII) using the Canadian Longitudinal Study on Aging (CLSA), together abbreviated as CLSA-SII. CLSA-SII was developed using standard methodology for developing other measures, including the multimorbidity resilience and successful aging indices. Associations of the CLSA-SII and four key outcome variables (life satisfaction scale, depression, perceived mental health, and perceived health) were performed using regression analyses. Associations between the CLSA-SII index and the four outcome variables resulted in statistically significant moderate associations in the expected direction, and are consistent with prior research, demonstrating good concurrent validity.
Longitudinal Trends in Quality of Life and Physical Function in Frail Older Dialysis Patients: A Comparison of Assisted Peritoneal Dialysis and In-Center Hemodialysis

Background: In-center hemodialysis (HD) has been the standard treatment for older dialysis patients, but reports suggest an associated decline in physical and cognitive function. Cross-sectional data suggest that assisted peritoneal dialysis (aPD), an alternative treatment, is associated with quality of life (QoL) outcomes that are comparable to in-center HD. We compared longitudinal changes in QoL between modalities. Methods: We enrolled 106 aPD patients, matched with 100 HD patients from 20 renal centers in England and Northern Ireland. Patients were assessed quarterly for 2 years using the Hospital Anxiety and Depression Scale (HADS), SF-12 physical and mental scores, symptom score, Illness Intrusiveness Rating Scale (IIRS), Barthel's score, and the Renal Treatment Satisfaction Questionnaire (RTSQ). Mixed model analysis was used to assess the impact of dialysis modality on these outcomes during follow-up. P values were adjusted for multiple significance testing. Results: Multivariate analysis showed no difference in any of the outcome measures between aPD and HD. Longitudinal trends in outcomes were also not significantly different. Higher age at baseline was associated with lower IIRS and RTSQ scores during follow-up. One-hundred and twenty-five (60.6%) patients dropped out of the study: 59 (28.6%) died, 61 (29.6%) withdrew during follow-up, and 5 (2.5%) were transplanted. Conclusions: Quality of life outcomes in frail older aPD patients were equivalent to those receiving in-center HD. Assisted PD is thus a valid alternative to HD for older people with end-stage kidney disease (ESKD) wishing to dialyze at home.
Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration

ABSTRACT Objectives: To observe whether Graphene oxide (GO) can absorb vitamin B12 (VB12) and Decellularized scaffold – acellular nerve allograft (ANA) modified GO-VB12 promote the repair of ischiadic nervus defects in a rat model. Methods: The adsorption of GO on vitamin and the optimum adsorption conditions were investigated by single factor experiment. The adsorption properties of the material were observed by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) to determine the success of adsorption on VB12. GO-VB12-ANA was prepared by vibration mixing method and bridged to injured ischiadic nervus. The nerve action potential, wet weight ratio of gastrocnemius muscle and the expression of GAP-43 were investigated by contrast test to detect its effect on nerve regeneration. Results: The optimized adsorption conditions for GO on VB12 solution were listed as follows: adsorbent dosage was 6 mg, shaking time was 70 min, the pH value was 6, the optimum concentration of VB12 was 50 mg/L and the theoretical saturated adsorption capacity was 21.51 mg/g. The nerve action potential, wet weight ratio of gastrocnemius muscle and the expression of GAP-43 in nerve fiber of GO-VB12-ANA group were close to the normal values and significantly higher than those of ANA and rotation group. Conclusions: Based on the adsorption function of GO on VB12, GO-VB12-ANA can promote regeneration of injured ischiadic nervus, providing the experimental basis for the clinical application of nanomaterials.