Miracle-dz/UniFAR-FTUs huggingface dataset:
Concordance between Thioacetamide-Induced Liver Injury in Rat and Human In Vitro Gene Expression Data

The immense resources required and the ethical concerns for animal-based toxicological studies have driven the development of in vitro and in silico approaches. Recently, we validated our approach in which the expression of a set of genes is uniquely associated with an organ-injury phenotype (injury module), by using thioacetamide, a known liver toxicant. Here, we sought to explore whether RNA-seq data obtained from human cells (in vitro) treated with thioacetamide-S-oxide (a toxic intermediate metabolite) would correlate across species with the injury responses found in rat cells (in vitro) after exposure to this metabolite as well as in rats exposed to thioacetamide (in vivo). We treated two human cell types with thioacetamide-S-oxide (primary hepatocytes with 0 (vehicle), 0.125 (low dose), or 0.25 (high dose) mM, and renal tubular epithelial cells with 0 (vehicle), 0.25 (low dose), or 1.00 (high dose) mM) and collected RNA-seq data 9 or 24 h after treatment. We found that the liver-injury modules significantly altered in human hepatocytes 24 h after high-dose treatment involved cellular infiltration and bile duct proliferation, which are linked to fibrosis. For high-dose treatments, our modular approach predicted the rat in vivo and in vitro results from human in vitro RNA-seq data with Pearson correlation coefficients of 0.60 and 0.63, respectively, which was not observed for individual genes or KEGG pathways.
Absorption-Enhanced Ultra-Thin Solar Cells Based on Horizontally Aligned p–i–n Nanowire Arrays

A horizontally aligned GaAs p–i–n nanowire array solar cell is proposed and studied via coupled three-dimensional optoelectronic simulations. Benefiting from light-concentrating and light-trapping properties, the horizontal nanowire array yields a remarkable efficiency of 10.8% with a radius of 90 nm and a period of 5 radius, more than twice that of its thin-film counterpart with the same thickness. To further enhance the absorption, the nanowire array is placed on a low-refractive-index MgF2 substrate and capsulated in SiO2, which enables multiple reflection and reabsorption of light due to the refractive index difference between air/SiO2 and SiO2/MgF2. The absorption-enhancement structure increases the absorption over a broad wavelength range, resulting in a maximum conversion efficiency of 18%, 3.7 times higher than that of the thin-film counterpart, which is 3 times larger in GaAs material volume. This work may pave the way for the development of ultra-thin high-efficiency solar cells with very low material cost.
Excessive Screen Media Use in Preschoolers Is Associated with Poor Motor Skills

Excessive screen media use exposure is a robust childhood predictor of sedentary behavior. The association between excessive exposure to sedentary behaviors (e.g., screen media use) and motor skills and how this association differs across sociodemographic strata is an important knowledge gap that needs to be addressed. The study aims to investigate the association of motor skills and screen media use in preschool children, taking into account sociodemographic variables, physical activity, and sleep profile. A cross-sectional survey of 926 children from 27 preschools was performed. The main outcome was defined as motor skills assessed using the general motor quotient (GMQ). Independent variables included sociodemographic variables, screen media use, screen habits, physical activity, and sleep duration. Logistic regression models were used to estimate the associations between the children's motor skills and each exposure factor. More than 55 percent of the children ate while watching television and 28 percent spent a long time watching television, playing video games, or using a computer, tablet, or cell phone. Excessive screen media use increased the risk of a low GMQ by 72 percent and inactivity in children increased the odds by 90 percent; sleep duration at night decreased the odds of a low GMQ by 51 percent and daytime sleep decreased the odds by 33 percent. Excessive screen media use has been associated with poor motor skills and increased physical inactivity in children, especially among those with prolonged exposure. Our findings can alert parents to the consequences of excessive screen media use and can motivate policymakers to encourage sports and other health-promotion strategies.
Comparative Effect of Rotary Microbrush Combined with Different Chemical Irrigants on Root Dentin Microhardness, Roughness and Bond Strength to an Epoxy-based Sealer.

PURPOSE This study evaluated the effect of 17% EDTA, 10% citric acid (CA), and 2% chlorhexidine (CHX) activated with a rotary microbrush (CanalBrush) on root dentin microhardness, roughness, and epoxy-based sealer bond strength. MATERIALS AND METHODS One hundred sixty single-rooted bovine incisors were instrumented and divided into 8 groups according to treatment: 1. 17% EDTA; 2. 17% EDTA+2% CHX; 3. 10% CA; 4. 10% CA+2% CHX; 5. 17% EDTA with CanalBrush; 6. 17% EDTA+2% CHX with CanalBrush; 7. 10% CA with CanalBrush; and 8. 10% CA+2% CHX with CanalBrush. Ten roots in each group were split into halves and submitted to microhardness and roughness analyses (n = 10). Following endodontic filling with AH Plus sealer, 10 roots in each group underwent push-out bond strength testing (n = 10). Data were analyzed using two-way ANOVA and Tukey's tests (α = 0.05). RESULTS All groups had similar microhardness values (p > 0.05) which was higher in the apical third than in the middle and cervical thirds (p < 0.05). The CanalBrush groups had higher roughness than the no-activation groups (p < 0.05), with significantly higher roughness in the cervical third than in the apical third (p < 0.05). All groups exhibited similar bond strength (p > 0.05), with the cervical third being higher, followed by the middle and apical thirds (p < 0.05). CONCLUSIONS Microbrush activation had a direct impact on dentin roughness and did not influence the dentin microhardness or the retention of epoxy-based sealer to the root canal.
Comparative studies of Danish Flavobacterium psychrophilum isolates: ribotypes, plasmid profiles, serotypes and virulence

Ribotyping and plasmid profiling were carried out on 299 Danish Flavobacterium psychrophilum isolates from farmed rainbow trout, Oncorhynchus mykiss (Walbaum). The isolates had been characterized biochemically and serologically in another study. The isolates were very homogeneous, 254 isolates had the same ribotype A (restriction enzyme EcoRI) and 284 isolates harboured one 3.3 kb plasmid. Seventy-five per cent of the F. psychrophilum isolates had ribotype A, one 3.3 kb plasmid and belonged to either serotype Th or Fd. Virulence studies with representatives of the dominant groups classified such isolates as virulent, and an extra small or large plasmid did not change the virulence level. A relationship between the serotypes Fd and Th, certain ribotypes, and virulence was found. The isolates belonging to serotype FpT and to ribotype B were less virulent. Only a few isolates with other ribotypes were found and these were also less virulent. Isolates that did not have the 3.3 kb plasmid were also less virulent. The virulent isolates all harboured the 3.3 kb plasmid; however, there was no clear correlation between virulence and plasmid content as a 3.3 kb plasmid was also found in less virulent isolates.
Seletividade e controle de plantas daninhas com oxyfluorfen e sulfentrazone na implantação de lavoura de café

This study aimed to evaluate oxyfluorfen and sulfentrazone selectivity and weed control in young coffee plantations (Coffea arabica cv. Red Catuai). Three trials were conducted in a randomized complete block design, with 10 treatments and four replicates. In the first and second trials, at 30 and 90 days after transplanting (DAT), respectively, two doses of oxyfluorfen (0.36 and 0.72 kg a.i. ha-1) and sulfentrazone (0.4 and 0.6 kg a.i. ha-1) were tested by spraying the herbicides directly into soil (with seedling protection) or onto the total area. The same herbicides and doses were used in the third trial, but at 300 DAT and only directed to the soil, using two sprayer nozzles with different drift potentials being tested. Two additional control plots were added: hand-weeding control and weedy treatments. Weeds present in the inter-row spaces were eliminated by mowing operations. Weeds and their densities were identified. The toxicity of the herbicides to the coffee plants and its effectiveness in controlling the weeds were assessed. The main weed occurring in the experimental area was Brachiaria decumbens. Visual symptoms of herbicide toxicity were observed only when the herbicides were applied in the entire area (trials 1 and 2), regardless of their doses and application times. At the third herbicide application trial, no visual symptoms of herbicide toxicity and no reductions in plant growth were observed, regardless of the herbicide, the dose and the sprayer nozzles tested. At all times, a very good weed control percentage was obtained, but selectivity occurred only when the herbicides were not applied onto the total area.
Oxidation of Quercetin by Salivary Components II. Effects of Quercetin on Reactive Oxygen Metabolism by Salivary Polymorphonuclear Leukocytes

Salivary polymorphonuclear leukocytes (SPMN) produce O2− and H2O2 reducing molecular oxygen, and quercetin is a phenolic compound found in vegetables and fruits. The aim of the present study was to elucidate the interaction between SPMN and quercetin. The oxygen consumption by SPMN, which was stimulated by phorbol myristate acetate (PMA), was suppressed by quercetin. Half-inhibition was observed at about 20 μM quercetin. During inhibition of the oxygen uptake, quercetin was oxidized; the oxidation was inhibited by superoxide dismutase and enhanced by horseradish peroxidase. Quercetin was taken up into SPMN and myeloperoxidase in SPMN could oxidize quercetin if H2O2 was provided. These results indicate that quercetin can inhibit the formation of O2− and H2O2 by PMA-stimulated SPMN and that the flavonol can scavenge O2− and H2O2 generated by the SPMN. Quercetin inhibited the growth of Porphyromonas gingivalis that might cause periodontal disease. According to the above results, quercetin seems able to suppress SPMN-dependent inflammation caused by reactive oxygen species inhibiting the growth of bacteria in the mouth.
American Cancer Society Guideline for Diet and Physical Activity for cancer prevention.

The American Cancer Society (ACS) publishes the Diet and Physical Activity Guideline to serve as a foundation for its communication, policy, and community strategies and, ultimately, to affect dietary and physical activity patterns among Americans. This guideline is developed by a national panel of experts in cancer research, prevention, epidemiology, public health, and policy, and they reflect the most current scientific evidence related to dietary and activity patterns and cancer risk. The ACS guideline focus on recommendations for individual choices regarding diet and physical activity patterns, but those choices occur within a community context that either facilitates or creates barriers to healthy behaviors. Therefore, this committee presents recommendations for community action to accompany the 4 recommendations for individual choices to reduce cancer risk. These recommendations for community action recognize that a supportive social and physical environment is indispensable if individuals at all levels of society are to have genuine opportunities to choose healthy behaviors. This 2020 ACS guideline is consistent with guidelines from the American Heart Association and the American Diabetes Association for the prevention of coronary heart disease and diabetes as well as for general health promotion, as defined by the 2015 to 2020 Dietary Guidelines for Americans and the 2018 Physical Activity Guidelines for Americans.
Phylogeny and Classification of the Family Lycidae (Insecta: Coleoptera)

Abstract. Lycidae, net winged beetles, have proved difficult to classify using morphological characters. Here, using a previously published molecular phylogeny, comparing the results with morphological data and re-analyzing previously published morphological data set, we propose a revised classification of Lycidae. All analyses support the monophyly of Lycidae, but phylogeny inferred from molecular data is in conflict with the current classification. The adult larviform females evolved in several lineages and never switched back to a winged form. Therefore, neotenic development of females is not a synapomorphy of Lycidae and the neotenic lineages do not form a basal paraphylum with respect to remaining Lycidae as previously proposed. As a consequence morphological similarities resulting from neoteny are homoplasies and cannot be used for definition of monophyletic lineages. The major result of this study is delineation of five basal clades, which are given subfamily rank: Libnetinae Bocak et Bocakova, 1990, stat. nov., Dictyopterinae Kleine, 1928, stat. nov., Lyropaeinae Bocak et Bocakova, 1989, Ateliinae Kleine, 1928 and Lycinae Laporte, 1836, sensu nov. Dexorinae Bocak et Bocakova, 1989, stat. nov. were not available for molecular analyses and their position is inferred from morphology alone. Further, Lycinae are redefined. Leptolycini are nested among Neotropical Lycini and render them paraphyletic. Slipinskiini are shown to group with Erotini. The distant position of Macrolycini and Dilophotini is demonstrated and Dilophotini are transferred from Calochrominae to Ateliinae. Four new tribes are proposed: Lycoprogenthini trib. nov. in Dictyopterinae, Alyculini trib. nov. and Antennolycini trib. nov. in Lyropaeinae, Dihammatini trib. nov. in Lycinae. Calochromini Lacordaire, 1857, stat. nov. and Leptolycini Leng et Mutchler, 1922, stat. nov. are lowered to the tribal status in Lycinae. The validity of Thonalmini Kleine, 1933 stat. rev. is re-established.
The molecular grouping of Fusarium isolates on cassava in Nigeria

The Fusarium isolates molecularly grouped in this experiment were isolated from the roots of three cassava genotypes (TMS 30572, TMS 4(2)1425 and TME-1) harvested in Ibadan derived savanna, Sabongidda-Ora humid forest and the Onne humid forest regions of Nigeria in 2004 and 2005. Isolates were previously identified using conventional morphological characters at the Plant Pathology Laboratory of the International Institute of Tropical Agriculture, Ibadan. Molecular grouping was done using amplified fragment length polymorphism at the Fusarium Laboratory of the Kansas State University, Kansas, USA. The molecular groupings agreed with the conventional morphological identification. Ten distinct amplified fragment length polymorphism (AFLP) groups of Fusarium were distinguishable, each group probably a distinct species, and many of them might represent previously undescribed Fusarium species. The two largest of the AFLP groups correspond to F. oxysporum and F. solani species complex. F. solani is an important root rot pathogen of cassava Nigeria.
Development of Sequence Characterized Amplified Region (SCAR) Marker for the Authentication of Bacopa monnieri (L.) Wettst

Aims: To develop Sequence Characterized Amplified Region (SCAR) marker for identification of Bacopa monnieri (L.) Wettst. Study design: Molecular biology tools for authentic identification of Bacopa monnieri . Methodology: RAPD-based SCAR marker was developed to identify Bacopa monnieri from its adulterant candidates namely Centella asiatica , Eclipta alba and Malva rotundifolia . 50 random primers were used for initial screening of different accessions of Bacopa monnieri, Eclipta alba and Malva rotundifolia. A putative 589 bp marker specific to Bacopa monnieri was identified using RAPD technique. This RAPD-amplicon was then sequenced and cloned. Based on the information of cloned sequences a pair of SCAR primers was designed. SCAR primers were then used for authentication of DNA samples of Bacopa monnieri and its adulterants. Market samples of Bacopa monnieri and Centella asiatica collected under the name of Brahmi was put to test with these primers. Results: Out of 50 random primers, only 14 primers were able to amplify the above plants. A 589 bp polymorphic band obtained with OPAA-3 primer which was specific to Bacopa monnieri accessions and not found in other adulterant candidates was selected. This band was eluted, cloned and further sequenced. A pair of SCAR primers (Bac F & Bac R) between 406 bp of 589 bp sequence of RAPD amplicon was designed. A single, bright, distinct band was obtained in Bacopa monnieri and not in the adulterants. Further validation was also done in the market samples. Conclusion: In essence, the study was to develop a RAPD-based SCAR marker for authentication of Bacopa monnieri . The SCAR marker was found to be useful for Research Article
Prenusinersen economic and health-related quality of life burden of spinal muscular atrophy

Objective To quantify the economic and health-related quality of life (HRQoL) burden incurred by households with a child affected by spinal muscular atrophy (SMA). Methods Hospital records, insurance claims, and detailed resource use questionnaires completed by caregivers were used to capture the direct and indirect costs to households of 40 children affected by SMA I, II, and III in Australia between 2016 and 2017. Prevalence costing methods were used and reported in 2017 US dollar (USD) purchasing power parity (PPP). The HRQoL for patients and primary caregivers was quantified with the youth version of the EQ-5D and CareQoL multiattribute utility instruments and Australian utility weights. Results The average total annual cost of SMA per household was $143,705 USD PPP for all SMA types (SMA I $229,346, SMA II $150,909, SMA III $94,948). Direct costs accounted for 56% of total costs. The average total indirect health care costs for all SMA types were $63,145 per annum and were highest in families affected by SMA II. Loss of income and unpaid informal care made up 24.2% and 19.8% respectively, of annual SMA costs. Three of 4 (78%) caregivers stated that they experienced financial problems because of care tasks. The loss in HRQoL of children affected by SMA and caregivers was substantial, with average caregiver and patient scores of 0.708 and 0.115, respectively (reference range 0 = death and 1 = full health). Conclusion Our results demonstrate the substantial and far-ranging economic and quality of life burden on households and society of SMA and are essential to fully understanding the health benefits and cost-effectiveness associated with emerging disease-modifying therapies for SMA.
Computational models identify several FDA approved or experimental drugs as putative agents against SARS-CoV-2

The outbreak of a novel human coronavirus (SARS-CoV-2) has evolved into global health emergency, infecting hundreds of thousands of people worldwide. In an effort to find antiviral medications, many computational groups have pursued the 3C-like protease of the virus, also known as main protease (Mpro), as a drug target. We have identified experimental data on the inhibitory activity of compounds tested against closely related (96% sequence identity, 100% active site conservation) protease of SARS-CoV and employed this data to build Quantitative Structure-Activity Relationships (QSAR) models for this dataset. We employed these models for virtual screening of all marketed, withdrawn, experimental, and investigational drugs from DrugBank, including compounds in clinical trials. Molecular docking and similarity search approaches were explored in parallel with QSAR modeling, but molecular docking failed to correctly discriminate between experimentally active and inactive compounds, so we did not rely on this approach in prospective virtual screening. As a result of our studies, we recommended 41 approved, experimental, or investigational drugs as potential agents against SARS-CoV-2 acting as putative inhibitors of Mpro>. Ten compounds with feasible prices were purchased and are awaiting the experimental validation. This manuscript will be updated once results are available and submitted for peer-review publication if compounds are found to be active in SARS-CoV-2 phenotypic screen.
PREDICTION OF THE INHIBITORY ACTIVITY OF BENZIMIDAZOLE DERIVATIVES AGAINST Bacillus spp.

In the present paper, the antibacterial activity of some 1-benzylbenzimidazole derivatives was evaluated against Gram-positive bacteria Bacillus spp. by using QSAR (quantitative structure-activity relationship). The tested compounds displayed in vitro antibacterial activity and minimum inhibitory concentration (MIC) was determined for all compounds. The lipophilicity descriptors of the studied compounds were measured by theoretical calculation (ClogP). The correlation between the MIC (log1/cMIC) and lipophilicity descriptors was investigated, and a mathematical model has been developed as a calibration model for predicting the antibacterial activity of this class of compounds. The quality of the model was validated by leave one out (LOO) technique as well as by the calculation of statistical parameters for the established model. The results of the present study may be useful for the designing of new benzimidazole derivatives that would be more potent against Bacillus spp.
Prediction of the hypertension risk in teenagers

Background Creation of a hypertension risk stratification model and development of an algorithm to detect hypertension in teenagers. Methods The study group consisted of 690 middle and high school students, aged 15–17 years, from the metropolitan area of Warsaw, Poland. Information concerning family history and presence of risk factors for cardiovascular disease was gathered. Three-time blood pressure measurements were taken during at least two separate visits, which were at least a week apart, using the auscultatory method, according to standard procedures. Anthropometric measurements included: body weight, height, arm, hip and abdominal circumference, skin-fold thickness measured on the rear surface of an arm, below the inferior angle of the scapula and at the belly. Following indexes were determined: body mass index, waist to hip ratio (WHR), waist to height ratio, hip to height ratio. Results A logistic regression model, describing the risk of hypertension in adolescents aged 15–17 was invented. π^(x)=eg^(x)1+eg^(x) where ĝ(x) = −0.097 × height+0.085 × weight+7.764 × WHR+1.312 × family hypertension. Family hypertension means presence of hypertension among members of the closest family. The formula was created, allowing the pre-selection of adolescents at risk of hypertension during screening. Next an algorithm for the detection of hypertension for practical use was proposed. Conclusions: Body weight, WHR and incidence of hypertension in the family are the strongest predictors of hypertension in teenagers. Proposed screening algorithm can be a useful tool for selecting teenagers at risk of hypertension and in need of specialized diagnostics and care.
Glutamine for Amelioration of Radiation and Chemotherapy Associated Mucositis during Cancer Therapy

Glutamine is a major dietary amino acid that is both a fuel and nitrogen donor for healing tissues damaged by chemotherapy and radiation. Evidence supports the benefit of oral (enteral) glutamine to reduce symptoms and improve and/or maintain quality of life of cancer patients. Benefits include not only better nutrition, but also decreased mucosal damage (mucositis, stomatitis, pharyngitis, esophagitis, and enteritis). Glutamine supplementation in a high protein diet (10 grams/day) + disaccharides, such as sucrose and/or trehalose, is a combination that increases glutamine uptake by mucosal cells. This increased topical effect can reduce painful mucosal symptoms and ulceration associated with chemotherapy and radiation in the head and neck region, esophagus, stomach and small intestine. Topical and oral glutamine seem to be the preferred routes for this amino acid to promote mucosal healing during and after cancer treatment.