Miracle-dz/UniFAR-FTUs huggingface dataset:
Development of a phage-based biosensor to detect Salmonella in food stuff

Foodand waterborne illnesses are a serious public health concern worldwide and have stimulated research aiming at a rapid and accurate detection of pathogens by applying biosensing technologies. Salmonella, Campylobacter and E. coli are some examples of pathogens that have an enormous impact on public health. Many publications have mentioned different type of biosensors for a broad range of bacteria. These methods may circumvent the limitations that conventional microbiological techniques have. Pathogens of interest need culture enrichment steps to reach the detection limit, a process that requires time, as well as laboratory technicians with expertise skills. Detection of pathogens at a very early stage is not as easy as it seems, due to the necessity to unite a set of characteristics that enable the development of an inexpensive and robust biosensor. The ideal biosensing system should be rapid and accurate and should combine specificity and sensitivity, leading to a marginal amount of false positive or negative results. As the biosensor is composed of two parts, a biological and a sensor element, the biorecognition element of choice plays a crucial role when creating the perfect biosensor. Bacteriophages (or simply phages) are viruses that specifically recognize bacteria and this characteristic can be used as a potential "key" to solve problems related with bacterial detection. Moreover, the easy and low cost production of these viruses combined with their stability in harsh environmental conditions make them excellent competitors with other biological elements (e.g. antibodies, enzymes). The use of phages as a therapeutic agent and as an interface in detection systems has gained special interest of the research community. In many laboratories, phage-based platforms have been developed; however only a few have broken the barrier and went to the market as a clinical diagnostic tool. Nowadays, the food sector still uses conventional methods to detect Salmonella in food stuff that, as mentioned before, take times and requires expert skills. Notwithstanding the great improvements in the detection area, biosensing systems still lack sensitivity and give erroneous results. Furthermore, problems related to the detection of bacteria in a viable but nonculturable (VBNC) state is one of the concerns that can give false negatives. VBNC bacteria are not able to grow
A New Criterion for Evaluating News Search Systems

Measuring the effectiveness of web search engines had been widely studied for the past fifteen years and different methods have been proposed by the researchers. These studies helps in identifying the most effective search engine and are useful for both users at the personal level and search engine vendors at the business level. So in this paper, first we extensively review traditional web search evaluation methods under four major categories and then discuss the urge for news search evaluation. We discuss possible criteria and quality measures foe evaluating web-based news search systems. And finally we evaluate four news search systems under a new criterioninformation richness, i. e. , extracting the useful contents from search result record (SRR).
Cyclic AMP‐dependent protein kinase (PKA) phosphorylates Ser362 and 467 and protein kinase C phosphorylates Ser550 within the M3/M4 cytoplasmic domain of human nicotinic receptor α4 subunits

Studies have suggested that the expression, translocation, and function of α4β2 nicotinic receptors may be modulated by α4 subunit phosphorylation, but little direct evidence exists to support this idea. The objective of these experiments was to identify specific serine/threonine residues on α4 subunits that are phosphorylated in vivo by cAMP‐dependent protein kinase and protein kinase C (PKC). To accomplish this, DNAs coding for human α4 subunits containing alanines in place of serines/threonines predicted to represent phosphorylation sites were constructed, and transiently transfected with the DNA coding for wild‐type β2 subunits into SH‐EP1 cells. Cells were pre‐incubated with 32Pi and incubated in the absence or presence of forskolin or phorbol 12,13‐dibutyrate. Immunoprecipitated α4 subunits were subjected to immunoblot, autoradiographic and phosphoamino acid analyses, and two‐dimensional phosphopeptide mapping. Results confirmed the presence of two α4 protein bands, a major band of 71/75 kDa and a minor band of 80/85 kDa. Phosphoamino acid analysis of the major band indicated that only serine residues were phosphorylated. Phosphopeptide maps demonstrated that Ser362 and 467 on the M3/M4 cytoplasmic domain of the α4 subunit represent major cAMP‐dependent protein kinase phosphorylation sites, while Ser550 also contained within this major intracellular loop is a major site for protein kinase C phosphorylation.
REMOTE SENSING OF OIL SLICKS

In airborne remote sensing, due to the differences between the material properties of oil and, water, an oil slick is detectable in most wavelength bands. The capability of mapping thin oil slicks varies with the wavelength used. The amount of cloud cover also has a great influence on the contrast and the optimum wavelength band for mapping. A significant relation also exists between the contrast and the thickness of the oil film in some parts of the spectrum, particularly in the green band, thermal infrared (IR) and the microwave region. The multiband signature is also related to the oil type.
DIANA, an end-to-end computational model of human word comprehension

This paper presents DIANA, a new computational model of human speech processing. It is the first model that simulates the complete processing chain from the on-line processing of an acoustic signal to the execution of a response, including reaction times. Moreover it assumes minimal modularity. DIANA consists of three components. The activation component computes a probabilistic match between the input acoustic signal and representations in DIANA’s lexicon, resulting in a list of word hypotheses changing over time as the input unfolds. The decision component operates on this list and selects a word as soon as sufficient evidence is available. Finally, the execution component accounts for the time to execute a behavioral action. We show that DIANA well simulates the average participant in a word recognition experiment.
Molecular cloning and analysis of the infectious hematopoietic necrosis virus genome and development of a subunit vaccine

approved: Dr. J.C. La:gyi -I Redacted for Privacy (i Complementary DNA (cDNA) clones were generated from the RNA genome of infectious hematopoietic necrosis virus (IHNV) by using random DNA oligomers to prime first strand synthesis. These clones were mapped to their respective locations on the genome and used to determine the nucleotide sequence of the nucleocapsid (N) gene and the intergenic region between the glycoprotein (G) and the nonvirion (NV) genes. Interesting features of the N gene sequence include short homologies with N genes of other rhabdoviruses at the 5' and 3' non-coding termini of the mRNA, as well as an exceptionally long 5' non-translated region of the mRNA suggesting a leader RNA may be coupled to the N mRNA. The IHNV N protein coding region shows no significant homology with other rhabdovirus N genes at either the nucleotide or amino acid level. The intergenic region between the G and NV genes also shares some sequence homology with those reported for other negative strand RNA viruses. In addition, plasmid vectors were constructed which expressed an antigenic determinant of the glycoprotein gene of IHNV as a fusion protein with the trpE protein of Escherichia coli. Insertion of Sau3AI fragments from the IHNV glycoprotein gene into trpE expression plasmids led to a fusion protein containing a hydrophilic segment of 104 amino acids from the middle portion of the viral glycoprotein. After induction with indoleacrylic acid, fusion proteins accumulated stably in the E. coli cells and accounted for approximately 10% of the total protein in the cell. Immunization trials in fish with the crude bacterial lysate containing the fusion protein have indicated that the trpE-glycoprotein fusion protein produced in bacteria does induce protective immunity. Molecular Cloning and Analysis of the Infectious Hematopoietic Necrosis Virus Genome and Development of a Subunit Vaccine
Simultaneous Tc-99m/Tl-201 imaging using energy-based estimation of the spatial distributions of contaminant photons

The advantages of simultaneous acquisition of Tc-99m and Tl-201 myocardial perfusion SPECT images can be fully realized only if the effects of the Tc-99m agent can be accurately removed from the Tl-201 image. The authors and others have previously reported simultaneous dual-isotope techniques for cardiac studies which make use of a third energy-window to estimate the Tc-99m scatter to be subtracted from the Tl-201 window. The authors have recently demonstrated, however, using a Monte Carlo program which simulates all details of the photon transport, that lead X-rays produced in the collimator may also contribute significantly to contamination in the Tl-201 window. The spatial distribution of the Tc-99m scattered photons differs from that of the lead X-rays. Therefore, the authors modified their correction technique so that, at each projection angle, the contaminant image to be subtracted from the image in the Tl-201 window was estimated as a linear combination of a scatter-window (90-110 keV) image, blurred by a 2D Gaussian filter, and the Tc-99m photopeak image, blurred by a different Gaussian filter. For simulated data which included 'liver' activity and non-uniform 'lung' attenuation, the improved dual-window subtraction technique provided a more accurate estimate of the true Tl-201 image, with less image noise, than did the single-window correction. <<ETX>>
Desert Puma: Evolutionary Ecology And Conservation Of An Enduring Carnivore

This is a scholarly monograph that presents the results of a 10-year field study of the ecology of the desert puma in the Chihuahua Desert of New Mexico. With the increasing recognition of the importance of top carnivores to the health and functioning of ecosystems. This book presents findings from one of the most comprehensive long-term studies of a top carnivore ever conducted.
A Novel Analysis Of The Application Of Foot-Print Recognition

: Foot print based recognition system is the most evolving technology in today’s world. Still this has been not mostly welcomed by the industries to use it in day-to-day basis. This is only because of the lack of efficiency in the application in which it is used. To bring out an efficient system design, first the technologies and the recognition rate are compared and analyzed till date. A survey to analyze the gap in the use of this technology in the real world is made up-to-date. There are previous survey works done up to the year 2011 and focuses mainly on the recognition rate of the technology used. In this, it mainly focuses on the papers mostly after 2011 and various application of it by analyzing every possible parameter and their drawbacks and benefits.
Supplemental Materials to : “Reality and risk: a refutation of S. Rendón’s analysis of the Peruvian Truth and Reconciliation Commission’s mortality study”

Between 2001 and 2006, the Program de Apoyo al Repoblamiento at the Peruvian Ministry of Women and Social Development (MIMDES) conducted its own survey of victims of the Peruvian conflict. The results of this “Census for Peace” were published by the Peruvian Government as a series of pdf documents which included lists of fatal victims (see, e.g., MIMDES, 2003, 2007). The 2003 MIMDES report documents 10,470 deaths, of which 66% were attributed to SLU. An update, in 2006, reported an additional 8,227 deaths and 2,390 disappearances, of which 71% were attributed to SLU. As part of an as-yet-unfinished project, we re-digitized these data and matched them with the TRC’s data. For our record linkage, we extracted the names of the victims from four pdf files. There were approximately 51,000 names in these files, which contained many duplicate records. After removing a small number of records that were not full names (e.g., ”son of,” ”wife of”) and linking the duplicate records, we found 20,468 unique, fully-identified records of victims of fatal violations in the MIMDES surveys. This is 619 records fewer than MIMDES reported. Since we needed to link the MIMDES records to the TRC’s, we had to obtain a version of the TRC’s confidential data including the names of the victims. We did so through direct agreement with its current steward, the Peruvian Ombudsman Office (Defensoŕıa del Pueblo). This database has some minimal differences with the anonymized data published by the TRC in 2003. This is due to an additional quality control step performed by the TRC which was not ready in time for the publication of the commission’s final report.
Studies on spreading depolarization in healthy and metabolically compromised brain tissues

Spreading depolarization (SD) is a slowly propagating wave of depolarization of neurons and glia. Since its original discovery in 1944, SD has been observed in various experimental settings, both in vitro and in vivo. SD has recently been detected in brains of human patients with various injuries, and a contribution of SD to deleterious consequences of acute brain injury is increasingly accepted.
Circularly polarized rectangular dielectric resonator antenna excited by an off-set conformal metal strip

A rectangular dielectric resonator antenna (DRA) has been excited by an off-set single conformal metal strip. By using such excitation technique two degenerate resonant modes, TExδ11 and TEy1δ1 of the rectangular DRA have been excited to achieve circular polarization (CP). A CP bandwidth of ~ 5.2% in conjunction with a wide impedance matching bandwidth of ~ 54% has been provided by the proposed DRA configuration. The antenna design has been simulated using computer simulation technology (CST). Antenna prototype has been built to verify the impedance matching bandwidth. Far field parameters have been optimized and verified using two simulation techniques in CST i.e. finite integration technique (FIT) and finite element method (FEM). A good agreement between the simulated and measured result has been observed for S11. Similarly a very good resemblance between the far field results from FIT and FEM have been demonstrated.
PHYSIOLOGICAL RESPONSES AND ECOLOGICAL SUCCESS OF LICHEN STEREOCAULON FOLIOLOSUM AND MOSS RACOMITRIUM SUBSECUNDUM GROWING IN SAME HABITAT IN HIMALAYA

The surface reflectance, chlorophyll fluorescence and water relation of lichen Stereocaulon foliolosum and moss Racomitrium subsecundum co-occurring in Himalayan environment are studied in vitro during dehydration and rehydration stages. In both the species total reflectance in RED and NIR region decreased during dehydration and showed maximum recovery during rehydration. Lichen reflected more light than moss during fresh as well as dehydrated condition. Reflectance parameters were observed to be different in both species. Fv/Fm in case of moss showed up to 93% recovery while lichen attained 100% after rehydration. Relative Water Content in both the species followed a similar pattern during dehydration and rehydration though moss desiccated and recovered faster. The lichen emerged as better desiccation tolerant according to derivatives of PV curve by having low Osmotic Potential at full turgor, low apoplastic water content, low relative water content at turgor loss and low elasticity modulus. Possible mutual advantages to lichen and moss by co-occurrence is discussed.
Effects of frying on the fatty acid composition in farmed and wild gilthead sea bream (Sparus aurata)

Summary The effects of frying in soybean (FWSO) and olive oils (FWOO) on the fatty acid composition of farmed and wild gilthead sea bream Sparus aurata were evaluated. The fat content increased with both frying treatments. However, after FWOO the moisture content of the fish was reduced to a greater extent than that in fish FWSO. The concentration of saturated fatty acids (SFA) decreased significantly during both frying processes (P < 0.01). However, the concentration of monounsaturated fatty acids (MUFA) increased significantly in fish fried in olive oil (P < 0.01). The fried fish contained a higher level of n-6 polyunsaturated fatty acids (PUFA) and a lower level of n-3 PUFA compared to raw fish. The n-3/n-6 ratio decreased in wild fish FWSO and FWOO from 0.94 ± 0.08 to 0.15 ± 0.01 and 0.15 ± 0.02, respectively. In farmed bream, the ratios decreased from 2.51 ± 0.03 to 0.18 ± 0.03 and 0.36 ± 0.01, respectively. The concentration of trans fatty acids decreased significantly in both fish types after frying (P < 0.05). The frying process widely affected the EPA and DHA content, limiting the positive effects of n-3 PUFA.
COMPARAÇÃO DO AJUSTE DOS MODELOS DE REGRESSÃO CENSURADOS EM DIFERENTES PACOTES ESTATÍSTICOS

Os modelos de regressao censurados ou modelos Tobit sao bastante usados na analise estatistica para modelar variaveis respostas que sao parcialmente observadas ou que possuem uma quantidade de valores agrupados em um valor limite. O campo de aplicacao desses modelos cobre diversas areas da ciencia, tais como econometria, biometria, ensaios clinicos dentre outros. No trabalho pioneiro de Tobin (Tobin, 1958), o modelo Tobit assume que o valor limite e zero. Logo, apenas valores positivos da variavel dependente sao efetivamente observados. Todavia, a inferencia no modelo Tobit leva em consideracao todo o conjunto de dados, tanto as respostas efetivamente observadas, quanto as censuradas. Para realizar a inferencia dos parâmetros do modelo Tobit sob a abordagem bayesiana e necessaria uma construcao de algoritmos para o calculo de integrais de alta dimensionalidade. Uma possibilidade para aproximar o valor de tais integrais e entende-las como sendo valores esperados de uma determinada distribuicao e considerar o metodo Monte Carlo via Cadeias de Markov (MCMC), cuja ideia e obter amostras e calcular estimativas amostrais da distribuicao. Outra possibilidade e considerar o metodo de Laplace, que aproxima a distribuicao alvo atraves de uma distribuicao normal com vetor de medias igual ao vetor composto pela moda da densidade alvo e matriz de covariância igual a matriz hessiana da densidade da distribuicao alvo. O desenvolvimento desse metodo, combinado com tecnicas de integracao numerica, resultam na abordagem INLA (Integrated Nested Laplace Approximations) (Rue et al., 2009). Em geral, essa abordagem gera resultados com um tempo de execucao menor quando comparado com os metodos MCMC (Rue et al., 2017; Meehan et al., 2018). Em termos metodologicos, o MCMC e um procedimento estocastico enquanto o INLA e uma aproximacao analitica.
Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer

Vanda Mimi Palmer is an orchid hybrid between Vanda Tan Chay Yan and Vanda tessellata. This orchid has won several international awards for its fragrance emission characteristic. To date, scientific studies on enzymes as well as their related genes and transcripts that are involved in floral scent production and biosynthesis are barely covered in fragrant orchids especially in vandaceous orchids. The aims of this study were to isolate full open reading frame (ORF) of O-methyltransferase transcript from Vanda Mimi Palmer (VMPOMT) and followed by sequence characterisation using bioinformatics tools. The size of successfully isolated full-length transcript of VMPOMT (Genbank accession no: KF278721) is 1,289bp, encoding for a polypeptide of 368 amino acid residues consisting of 1,104bp open reading frame (ORF) flanked by 2bp of 5'-untranslated region (UTR) and 182bp of 3'-UTR including a poly-A tail. Sequence analyses using bioinformatics tools have shown that VMPOMT has a conserved motif for O-methyltransferase group as found in OMT sequences from other plants that might be involved in methylation reactions in fragrance compounds biosynthesis in this orchid. Besides that, VMPOMT is predicted to be localised in cytoplasm instead of plastid due to the absence of chloroplast transit peptide in the sequence. Thus, sequence analyses on VMPOMT using several bioinformatics tools might contribute to the understanding of fragrance biosynthetic pathways in Vanda Mimi Palmer as well as in other fragrant orchids
Analysis of single cell gene expression of mESC and MEF by NGS

Gene expression development during cell dierentiation is a key factor to understand the mechanism of development. However, conventional gene expression analysis cannot distinguish among individual cell expression. In this paper, we re-analyze single cell gene expression measurements obtained by next gene sequencing technology during dierentiation from mouse ES cell to MEF.
Including Generative Mechanisms in Project scheduling using Hybrid Simulation

Scheduling is central to the practice of project management and a topic of significant interest for the operations research and management science academic communities. However, a rigour-relevance gap has developed between the research and practice of scheduling that mirrors similar concerns current in management science. Closing this gap requires a more accommodative philosophy that can integrate both hard and soft factors in the construction of project schedules. This paper outlines one interpretation of how this can be achieved through the combination of discrete event simulation for schedule construction and system dynamics for variable resource productivity. An implementation was built in a readily available modelling environment and its scheduling capabilities tested. They compare well with published results for commercial project scheduling packages. The use of system dynamics in schedule construction allows for the inclusion of generative mechanisms, models that describe the process by which some observed phenomenon is produced. They are powerful tools for answering questions about why things happen the way they do, a type of question very relevant to practice.
Development of a Multi-purpose Sauce using Kimchi

In this study, we explored the characteristics of a Chinese cabbage kimchi sauce, which was made from natural pigments, according to the preservation period. There were no significant changes in pH and total acidity and the color of the sauce with added natural pigments demonstrated stability. Also, vitamin C and the change of viscosity decreased as the preservation period increased. A although the number of lactic acid bacteria decreased over a 20 day period, a certain level was sustained for 91 days. Lastly, the sensory properties of the sauce were not significantly different according to the preservation period.
A Case Study on Marketing Strategy of Xiaomi

With the intense development and growth of China's mobile communication and technology industry, many manufacturers have come up to take the advantage of growing market of mobile phone users. Approximately 1 billion people own a smartphone. This has led to birth of many of the world’s famous mobile phone brands. One of the major brand, emerged in 2010 was Xiaomi. In this paper, background of Xiaomi’s, its marketing strategies, business models have been presented. Also, comparative analysis of marketing strategy of Xiaomi and other manufacturers has been done to understand the reasons for the market success and the exponential growth exhibited by the company. The current state of information available through various platforms has been used to perform the study. It has been inferred that, the primary reason for this exceptional growth has been the product quality at cheaper rate with a focus on customer feedback and requirements. The testimony to this fact is its ever expanding portfolio into various domains such as smart TVs, Speakers, home appliances, personal computers to name a few.