Miracle-dz/UniFAR-FTUs huggingface dataset:
Biological Properties of Cell Lines Derived from Moloney Virus-Induced Sarcoma

Two cell lines derived from a primary MSV-M-induced tumor in a BALB/c mouse were studied. One line (MS-2) was subject only to continuous tissue culture transfer (tct). After 21 tct, MS-2 cells produced progressive tumors (MS-2 tumors) in syngeneic hosts. The second cell line (MS-2T) was established by cultivation of a MS-2 tumor. The ability to produce progressive tumors decreased with increased number of tct, in both cell lines. The virus content of MS-2 and MS-2T cells was very low, as shown by uridine incorporation and electron microscopy. Immunofluorescence tests demonstrated that antigens different from the viral MSV-M antigens were present on the cell lines, and that antigenic changes occurred with increased number of tct. Serum of mice bearing progressive MS-2 tumors reacted with MS-2T cells when these cells produced progressive tumors and did not react with MS-2 cells when they produced regressing tumors. MS-2 cells producing regressing tumors reacted with serum from mice in which the MS-2 tumor had regressed and with serum from mice immunized with MS-2T cells at late tct when they were poorly oncogenic. The antigenic changes seemed, therefore, to parallel the decrease of malignancy. A chromosomal analysis carried out on MS-2 and MS-2T cells, when both produced progressive tumors, showed a modal number of 48 and 44, respectively. MS-2T cells showed a large acrocentric chromosome. In contrast, the MS-2 cells at late tct, when they gave regressing tumors, showed a modal number of 60 and a wide range of distribution of chromosome number.
Regularly occurring periods of eye motility, and concomitant phenomena, during sleep.

obtain their surface in square centimeters. This simple method provides a means by objective measurements to make evident changes in the surface of wounds that are not apparent to the naked eye. Figure 1 shows the observations recorded with this method in a man of 42 years of age with hemiplegia and a decubital ulcer over the right buttock. The clinicians who had observed this wound daily had not noticed any remarkable change; however, it is quite obvious that the wound grew larger each time the treatment was changed, and that the use of an antibiotic was followed by a particularly striking enlargement of the lesion. In this instance the procedure of projection and gravimetric planimetry was repeated by different operators and a variation of ±5% was found (indicated by a cross-hatched area on Fig. 1). Figure 2 shows the same type of observation in a woman with hemiplegia and a decubital ulcer. This patient died from septicemia, and the decubital ulcer worsened with the general condition of the patient. A method of gravimetric planimetry by standard photographs offers a means to study the course of surface wounds more accurately than by clinical observation or by the pictorial record alone. References
Human Parvovirus B19 Induces Cell Cycle Arrest at G2 Phase with Accumulation of Mitotic Cyclins

ABSTRACT Human parvovirus B19 infects specifically erythroid progenitor cells, which causes transient aplastic crises and hemolytic anemias. Here, we demonstrate that erythroblastoid UT7/Epo cells infected with B19 virus fall into growth arrest with 4N DNA, indicating G2/M arrest. These B19 virus-infected cells displayed accumulation of cyclin A, cyclin B1, and phosphorylated cdc2 and were accompanied by an up-regulation in the kinase activity of the cdc2-cyclin B1 complex, similar to that in cells treated with the mitotic inhibitor. However, degradation of nuclear lamina and phosphorylation of histone H3 and H1 were not seen in B19 virus-infected cells, indicating that the infected cells do not enter the M phase. Accumulation of cyclin B1 was persistently localized in the cytoplasm, but not in the nucleus, suggesting that B19 virus infection of erythroid cells raises suppression of nuclear import of cyclin B1, resulting in cell cycle arrest at the G2phase. The B19 virus-induced G2/M arrest may be the critical event in the damage of erythroid progenitor cells seen in patients with B19 virus infection.
Specifying Computer Systems with Probabilistic Sequence Diagrams

Probability often plays a major role in the specification of computer systems, for a number of reasons. Probabilistic requirements are, of course, essential for specifying applications such as games of chance or probabilistic algorithms. Soft real-time requirements are probabilistic requirements that are very important when we are interested in the performance characteristics of a system. Probabilistic requirements can be used when a certain amount of undesirable behavior (such as a message getting lost in a communication medium) has to be accepted for technical or economical reasons. Probability in specifications can also be used to model the system environment, or as an abstraction from detailed criteria for making a deterministic choice. This thesis proposes a specification language that allows probabilistic requirements to be expressed at the same level as other requirements. The language is based on UML sequence diagrams. Unlike UML sequence diagrams, however, the language is given a formal (denotational) semantics, and is supported by formal definitions of refinement and system compliance. Thereby, we remove ambiguity of specifications and facilitate formal reasoning about systems before they are built, as well as verification of systems against specifications. The combination of the specification language, the refinement relations, and the compliance relations is called probabilistic STAIRS, or pSTAIRS. On top of pSTAIRS, we propose a language that allows us to model the trust of actors, the influence of this trust on the choices of action made by the actors, and the actual system behavior. When analyzing systems with respect to risk or other concerns, such information is important in cases where the critical system behavior depends on choices made by actors based on trust. UML sequence diagrams focus on communication, which is essential in most computer systems today. They seem to be easy to understand at an intuitive level, and are much used in the software industry. By basing our languages on UML sequence diagrams, we hope to reduce the obstacles to adopting the languages in practical settings.
Absolute position calculation for a desktop mobile robot based on three optical mouse sensors

ArmAssist is a wireless robot for after stroke upper limb rehabilitation. In this paper, we describe a method based on artificial landmark navigation system. The navigation system is only based in three optical mouse sensors. This enables to build a cheap but reliable position sensor. Two of the sensors are the data source for odometry calculations, and the third optical mouse sensor takes very low resolution pictures of a custom designed mat. These pictures are processed by an optical symbol recognition algorithm which will estimate the orientation of the robot and recognize the landmarks placed on the mat. The data fusion strategy is described to detect the misclassifications of the landmarks in order to fuse only the reliable information. The orientation given by the OSR algorithm is used to improve significantly the odometry and the recognition of the landmarks is used to reference the odometry to a absolute coordinate system.
Ontogeny of human hepatic cytochromes P450

Significant changes in drug‐metabolizing enzyme (DME) expression occur during ontogeny. Such changes can have a profound effect on therapeutic efficacy in the fetus and child, as well as the risk for adverse drug reactions. To gain a better understanding of DME ontogeny, enzyme contents for six key cytochromes P450 were measured in 240 human liver samples representing ages from 8 weeks gestation to 18 years. Where possible, both quantitative western blotting and activity assays with probe substrates were performed. Although oversimplified, the DME can be grouped into one of three categories. As typified by CYP3A7, some enzymes are expressed at their highest level during the first trimester and either remain at high concentrations or decrease during gestation and are silenced or expressed at low levels within 1–2 years after birth. These data cause one to query whether these enzymes have an important endogenous function. Representatives of a second group, CYP3A5 and CYP2C19, are expressed at relatively constant levels throughout gestation. Postnatal increases in CYP2C19 are observed within the first year, but not for CYP3A5. CYP2C9, 2E1, and 3A4 are more typical of a third group of enzymes that are not expressed or are expressed at low levels in the fetus with the onset of expression generally in either the second or third trimester. Substantial increases in expression are observed within the first 1–2 years after birth; however, considerable interindividual variability is observed in the immediate postnatal (1–6 months) onset or increase in expression of these enzymes, often resulting in a window of hypervariability. © 2007 Wiley Periodicals, Inc. J Biochem Mol Toxicol 21:169–175, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20179
Stability, blood partition and plasma protein binding of ipriflavone, an isoflavone derivative.

It is generally recognized that the partition between plasma and blood cells, the immediate centrifugation of blood samples after collection for the measurement of 'true' in vivo concentrations and free drug concentrations in plasma are important determinants of the pharmacokinetics and/or pharmacodynamics of drugs. Therefore, the stability, blood partition between plasma and blood cells, and factors influencing the binding of ipriflavone to 4% human serum albumin (HSA) using an equilibrium dialysis technique were evaluated. Ipriflavone was unstable in rat liver homogenate and various pH solutions ranging from 1 to 13, except pH 8, rat blood and plasma and human plasma when incubated in a water-bath shaker for 24 h kept at 37 degrees C and at a rate of 50 oscillations/min. The recoveries of spiked amounts of ipriflavone at 24 h pH solutions ranging from 1 to 12 were 67.0, 78.1, 87.9, 89.6, 84.2, 87.4, 85.5, 99.3, 88.0, 76. 6, 79.4 and 81.5%, respectively. Ipriflavone was very unstable in pH 13 solution; only 0.814% of ipriflavone was recovered after 30 min incubation. Ipriflavone was stable for up to 3 h incubation in human gastric juices. Ipriflavone reached equilibrium fast (within 30 s of being mixed manually) between plasma and blood cells and the equilibrium plasma/blood cells partition ratios were independent of the initial rabbit blood concentrations of ipriflavone: 0.2, 2, and 10 microg/mL; the values were in the range of 0.900-2.45. The binding of ipriflavone to 4% HSA was 96.6+/-0.407% at ipriflavone concentrations ranging from 2 to 100 microg/mL, but it was dependent on HSA concentrations (0.5-6%), incubation temperature (4, 22 and 37 degrees C), 'the buffer' pHs (5.8, 6.4, 7.0, 7.4 and 8.0), and addition of salicylic acid (150-300 microg/mL) and sulphisoxazole (100-300 microg/mL). However, the binding was independent of buffers containing various concentrations of chloride ion (0-0.546%), glucose (0 and 5%), alpha-1-acid glycoprotein (0-0.32%) and heparin (0-40 U/mL), and addition of its metabolites (M1 and M5, 5 microg/mL).
Mathematical model for wireless sensor nodes security

Wireless sensor networks have recently emerged as an important means to study and interact with physical world. In previous era, castles were surrounded by moats (deep trenches, filled with water, and even alligators) to prevent or discourage intrusion attempts. Today one can replace such barriers with stealthy and wireless sensors. In this paper, we develop mathematical foundations model using barriers concept to design secure wireless sensors nodes. Security becomes one of the major concerns when there are potential attacks against sensor network nodes. Thus we have designed fundamental security in disk shaped to provide basic security elements that can be implemented in various sensor nodes. The mathematical models introduced in this paper are flexible and efficient to be embedded in sensor nodes and can create a suitable nodes components security in hostile environments. We also demonstrate how these nodes can be deployed in wall and belt form to fulfill their tasks.
Characterization of a dual band metasurface antenna with broadside and isoflux circularly polarized radiation patterns

In this paper, we present the theoretical considerations for the implementation of a single layer anisotropic metasurface (MTS) antenna capable to provide different circularly polarized radiation patterns at two frequencies at Ku band (13.5GHz and 16GHz). Circular metallic subwavelength patches with a groove through the diameter are employed to synthesize the proposed MTS configuration. At lower band broadside radiation with a maximum directivity of 26.5dBs is obtained, with 28dBs cross polar field component. At higher band, isoflux-shaped radiation pattern with a drop-off angle around 33° and 10dBs cross polar field is obtained.
Hybrid revascularization in multivessel coronary artery disease.

OBJECTIVES Minimally invasive hybrid revascularization (MIHR) by means of the left mammary artery on the left anterior descending artery [minimally invasive direct coronary artery bypass (MIDCAB)] combined with percutaneous coronary interventions (PCI) stenting may be an alternative to conventional coronary artery bypass grafting through sternotomy or multiple PCI. The purpose of this study is to retrospectively evaluate the long-term outcomes of this strategy. METHODS Since May 1997 up to January 2011, 810 MIDCAB have been performed as isolated revascularization in 644 patients. Since 2004, MIDCAB, as a part of hybrid revascularization, was associated with PCI in 166 patients. RESULTS In the MIDCAB group, mean age was 64.6 ± 12.0, with 83.8% males. Two-vessel disease was 62.4%, three-vessel disease 37.6%. Overall mortality was 0.24%, perioperative acute myocardial infarction-1.6%, early reoperation-0.74%, reopening for bleeding-1.2%, case rate of haemotrasfusion-3.1%, with a mean hospital postoperative stay of 4 ± 2.5 days. Postoperative angiographic control prior to PCI and in symptomatic patients showed patent left internal mammary artery in 100% of cases. PCI was performed in 166 patients, 64.2% before MIDCAB and 35.8% after surgery (interval 2.2 ± 1.3 months). The mean follow-up in the MIDCAB group was 8.4 ± 3.2 years. In the MIHR group, at the mean follow-up of 4.5 ± 2.3 years, freedom from related cardiac death was 93% with freedom from cardiac reintervention of 83%. CONCLUSIONS Our 13-year experience with MIDCAB demonstrates that the operation is safe and associated with a very low incidence of early and late complications. The hybrid approach provided excellent long-term outcome in terms of freedom from cardiac death and reoperation. Accurate patient selection, as well the timing of the hybrid procedure, is mandatory to optimize surgical and PCI results.
Thalamic atrophy and cognition in multiple sclerosis

Objectives: Recent studies have indicated that brain atrophy is more closely associated with cognitive impairment in multiple sclerosis (MS) than are conventional MRI lesion measures. Enlargement of the third ventricle shows a particularly strong correlation with cognitive impairment, suggesting clinical relevance of damage to surrounding structures, such as the thalamus. Previous imaging and pathology studies have demonstrated thalamic involvement in MS. In this study, we tested the hypothesis that thalamic volume is lower in MS than in normal subjects, and that thalamic atrophy in MS correlates with cognitive function. Methods: We studied 79 patients with MS and 16 normal subjects. A subgroup of 31 MS subjects underwent cognitive testing. The thalamus was segmented in whole from three-dimensional MRI scans. We also determined whole brain atrophy (brain parenchymal fraction), third ventricular width, and whole brain T2-weighted (fluid-attenuated inversion recovery) hyperintense, T1 hypointense, and gadolinium-enhanced lesion volumes. Results: Normalized thalamic volume was 16.8% lower in the MS group (p < 0.0001) vs controls. Cognitive performance in all domains was moderately to strongly related to thalamic volume in the MS group (r = 0.506 to 0.724, p < 0.005), and thalamic volume entered and remained in all regression models predicting cognitive performance. Thalamic volume showed a weak relationship to physical disability score (r = −0.316, p = 0.005). Conclusion: These findings suggest that thalamic atrophy is a clinically relevant biomarker of the neurodegenerative disease process in multiple sclerosis.
Efficient synthetic method for ethyl (+)-(2S,3S)-3-[(S)-3-methyl- 1-(3-methylbutylcarbamoyl)butylcarbamoyl]-2-oxiranecarb oxylate (EST), a new inhibitor of cysteine proteinases.

Ethly (+)-(2S, 3S) -3- [(S) -3-methyl-1- (3-methylbutylcarbamoyl) butylcarbamoyl] -2- oxirane-carboxylate (EST ; la) is expected to be useful as an oral therapeutic agent for muscular dystrophy on the basis of its potent inhibitory activities against the cysteine proteinases involved in the myofibrillar protein degradation that occurs in the disease. Through extensive investigations aimed at developing a new synthetic method for la that would be suitable for industrial application, it has been found that L-arginine can be used as a new, efficient resolving agent to obtain optically pure L-trans-epoxysuccinic acid (3a), and the active ester method using p-nitrophenol is very effective in the coupling reaction of ethyl L-trans-epoxysuccinate (7a) and L-leucine isoamylamide (8a) because of the extremely low formation of by-products.To examine the contribution of the stereochemistry of the trans-epoxysuccinic acid and leucine moieties to the inhibitory activity against cysteine proteinases, the diastereomers (lb-d) of la were synthesized by a similar method and the rate constants of inactivation of papain by la-d were measured. Compound la, having L-trans-epoxysuccinic acid and L-leucine moieties, showed the most potent activity among them.
Socioeconomic differentials in life expectancy and years of life lost in Victoria, 1992-1996.

While the public has growing expectations of health services, and the repertoire of health services to respond to these demands is expanding, governments are under pressure to justify their allocation of health resources. Expressed intentions to base decision-making on health outcomes will remain rhetoric, however, unless adequate tools to measure health outcomes are used. In response to this need for comparable information on health outcomes, the Public Health Division of the Department of Human Services in Victoria has undertaken a body of work to assist decision-making on health resource allocation in Victoria.
Determination of the Attitude of 3D Objects from a Single Perspective View

A method for finding analytical solutions to the problem of determining the attitude of a 3D object in space from a single perspective image is presented. Its principle is based on the interpretation of a triplet of any image lines as the perspective projection of a triplet of linear ridges of the object model, and on the search for the model attitude consistent with these projections. The geometrical transformations to be applied to the model to bring it into the corresponding location are obtained by the resolution of an eight-degree equation in the general case. Using simple logical rules, it is shown on examples related to polyhedra that this approach leads to results useful for both location and recognition of 3D objects because few admissible hypotheses are retained from the interpolation of the three line segments. Line matching by the prediction-verification procedure is thus less complex. >
DSAP Shared Task : Ensembles of Deep Architectures for Valence-Arousal Prediction

This paper introduces Mainiway AI Labs submitted system for the IJCNLP 2017 shared task on Dimensional Sentiment Analysis of Chinese Phrases (DSAP), and related experiments. Our approach consists of deep neural networks with various architectures, and our best system is a voted ensemble of networks. We achieve a Mean Absolute Error of 0.64 in valence prediction and 0.68 in arousal prediction on the test set, both placing us as the 5th ranked team in the competition.
Thesis for The Degree of Doctor of Philosophy High Efficiency Microwave Amplifiers and SiC Varactors Optimized for Dynamic Load Modulation

The increasing use of mobile networks as the main source of internet connectivity is creating challenges in the infrastructure. Customer demand is a moving target and continuous hardware developments are necessary to supply higher data rates in an environmentally sustainable and cost effective way. This thesis reviews and advances the status of realizing wideband and high efficiency power amplifiers, which will facilitate improvements in network capacity and energy efficiency. Several demonstrator PAs are proposed, analyzed, designed, and characterized: First, resistive loading at higher harmonics in wideband power amplifier design suitable for envelope tracking (ET) is proposed. A 40 dBm decade bandwidth 0.4–4.1 GHz PA is designed, with 10–15 dB gain and 40–62% drain efficiency. Its versatility is demonstrated by digital pre-distortion (DPD) linearized measurements resulting in adjacent channel leakage ratios (ACLR) lower than −46 dBc for various downlink signals (WCDMA, LTE, WiMAX). Second, a theory for class-J microwave frequency dynamic load modulation (DLM) PAs is derived. This connects transistor technology and load network requirements to enable power-scalable and bandwidth conscious designs. A 38 dBm PA is designed at 2.08 GHz, maintaining efficiencies >45% over 8 dB of output power back-off (OPBO) dynamic range. From this pre-study a fully packaged 86-W peak power version at 2.14 GHz is designed. ACLR after DPD is −46 dBc at a drain efficiency of 34%. For DLM PAs there is a need for varactors with large effective tuning range and high breakdown voltage. For this purpose, SiC Schottky diode varactors are developed with an effective tuning range of 6:1 and supporting a 3:1 tuning ratio at 36 V of RF swing. Nonlinear characterization to enable Qfactor extraction in the presence of distortion is proposed and demonstrated by multi-harmonic active sourceand load-pull, offering insights to tunable network design. Third, a method to evaluate and optimize dual-RF input PAs, while catering to higher harmonic conditions and transistor parasitics, is proposed. The method is validated by a PA design having a peak power of 44 ± 0.9 dBm and 6 dB OPBO PAE exceeding 45% over a 1–3 GHz bandwidth. The results in this thesis contribute with a novel device and analysis of high efficiency and wideband PAs, aiding in the design of key components for future energy efficient and high capacity wireless systems.
Generation of a conditional allele for the mouse endothelial nitric oxide synthase gene

Mice with endothelial nitric oxide synthase (eNOS) deletions have defined the crucial role of eNOS in vascular development, homeostasis, and pathology. However, cell specific eNOS function has not been determined, although an important role of eNOS has been suggested in multiple cell types. Here, we have generated a floxed eNOS allele in which exons 9–12, encoding the sites essential to eNOS activity, are flanked with loxP sites. Mice homozygous for the floxed allele showed normal eNOS protein levels and no overt phenotype. Conversely, homozygous mice with Cre‐deleted alleles displayed truncated eNOS protein, lack of vascular NO production, and exhibited similar phenotype to eNOS knockout mice, including hypertension, low heart rate, and focal renal scarring. These findings demonstrate that the floxed allele is normal and it can be converted to a non‐functional eNOS allele through Cre recombination. This mouse will allow time‐ and cell‐specific eNOS deletion. genesis 50:685–692, 2012. © 2012 Wiley Periodicals, Inc.