Miracle-dz/UniFAR-FTUs huggingface dataset:
A Comparative Study of the Distribution of Spiritual Gifts: Among Seminary Students and Other Graduate and Undergraduate Students at Andrews University

Problem Recently, considerable interest in spiritual gifts has developed and many scholars have attempted to measure them. This study was to determine whether spiritual gifts distribution is related to a specific demographic profile, and discriminates between certain groups. Method The New Spiritual Gift Inventorv provided scores for five spiritual gifts clusters, and three statistical procedures were employed to analyze the data gathered from 3 35 students. Results These results were obtained: 1. The clusters do not discriminate between students solely on the basis of academic classification. 2. Seminarians are stronger on the Teacher cluster than other students. 3. Males are stronger on the Teacher cluster and weaker on the Helper cluster than females. 4. Older students are stronger on the Teacher cluster than younger students. 5. A particular demographic profile is associated with a specific combination of gifts. Conclusions These differences among the groups suggest that believers should expect such differences in local congregations.
Androgen insufficiency in women: diagnostic and therapeutic implications

L.M. Rivera-Woll, M.Papalia, S.R. Davis and H.G. Burger The Jean Hailes Foundation Research Unit, 173 Carinish Road, Clayton, Victoria, Australia, 3168, The Jean Hailes Foundation NHMRC Centre of Clinical Research Excellence, Department of Obstetrics and Gynaecology, Monash University, Clayton, Victoria, Australia, 3168 and Prince Henry’s Institute of Medical Research, 246 Clayton Road, Clayton, Victoria, Australia, 3168
Prediction of prognosis in axillary lymph node positive breast cancer patients: A statistical study

This work has identified variables of importance to short‐ and long‐term prognosis in 97 node positive breast cancer patients followed for a minimum of 98 months. The diameter of the primary tumour (⩽3 cm/>3 cm) is shown to be an important prognostic variable. Its addition to the presence/absence of tumour cells in the efferent nodal vessels and the mean nuclear area of the tumour cells gave correct prediction of the disease outcome, 60 and 98 months after the operation, in 83 and 80 per cent of the patients respectively. Although the number of tumour‐bearing nodes is shown to be a strong variable, more information was gained from the tumour diameter. Histological grade was only of value for 5‐year prognosis. Factors that did not add information of prognostic value in these circumstances were identified. The proposed models demonstrate the great heterogeneity within this group of patients.
Bacterial community dynamics during the bioremediation of diesel contaminated soil using brewery spent grain

Brewery spent grain (BSG) has been shown to be nutrient rich and support the growth of microbes. It has thus been exploited in studies for the bioremediation of hydrocarbon contaminated soils. However, previous studies using BSG, have tended to investigate contaminant reduction and microbial growth. The associated bacterial community dynamics, metabolites, catabolic genes, and any changes over time have not been investigated. This knowledge could proffer a more informed rate enhancing strategy to optimise the remediation process. The study reported here has undertaken research to evaluate the bioremediation potential of BSG in diesel contaminated soil in terms of bacterial population dynamics, bacterial metabolic potential in key hydrocarbon degradation pathways, presence and abundance of degradation genes and shifts in bacterial populations towards known hydrocarbon degraders. It has also evaluated the breakdown of the diesel and attempted to correlate this with changes in bacterial populations. The results show that BSG accelerates the biodegradation of diesel contamination in soils. Bacterial counts increased significantly in soil samples with BSG added, from log 3.9 up to log 5.6. The alkB gene for alkane degradation and catA and xylE genes for aromatic hydrocarbon degradation, were significantly higher in the microbial community of soils treated with BSG; implying that BSG supported the proliferation of both aromatic and aliphatic hydrocarbon degrading microbes possessing these genes. Also, the metagenomic evaluation of bioremediation over time, adopted in this study, revealed shifts in bacterial populations in favour of Flavobacterium, Pseudomonas and Acinetobacter species and the latter two were shown to be intrinsic populations of the BSG control treatment. Of these species, Acinetobacter spp. were associated with the xylE gene while Pseudomonas putida was associated with both the catA and alkB genes; thus enhancing both aliphatic and aromatic hydrocarbon biodegradation. The BSG control treatment was also shown to harbour five other known hydrocarbon degrading bacterial species.
Injection and Self‐Assembly of Bioinspired Stem Cell‐Laden Gelatin/Hyaluronic Acid Hybrid Microgels Promote Cartilage Repair In Vivo

In this paper, a novel bioinspired stem cell‐laden microgel and related in vivo cartilage repair strategy are proposed. In particular, herein the preparation of new stem cell‐laden microgels, which can be injected into the chondral defect site in a minimally invasive way, and more importantly, capable of in situ self‐assembly into 3D macroporous scaffold without external stimuli, is presented. Specifically, thiolated gelatin (Gel‐SH) and vinyl sulfonated hyaluronic acid (HA‐VS) are first synthesized, and then stem cell‐laden gelatin/hyaluronic acid hybrid microgels (Gel‐HA) are generated by mixing Gel‐SH, HA‐VS, and bone mesenchymal stem cells (BMSCs) together via droplet‐based microfluidic approach, followed by gelation through fast and efficient thiol‐Michael addition reaction. The encapsulated BMSCs show high viability, proliferation, and chondrogenic differentiation potential in the microgels. Moreover, the in vitro test proves that BMSC‐laden Gel‐HA microgels are injectable without sacrificing BMSC viability, and more importantly, can self‐assemble into cartilage‐like scaffolds via cell–cell interconnectivity. In vivo experiments further confirm that the self‐assembled microgels can inhibit vascularization and hypertrophy. The Gel‐HA microgels and relevant cartilage repair strategy, i.e., injecting BMSC‐laden microgels separately and reconstructing chondral defect structure by microgel self‐assembly, provides a simple and effective method for cartilage tissue engineering and regenerative medicine.
AN EXAMINATION OF THE HEART RATE AND HYPOTHALAMIC- PITUITARY-ADRENAL AXIS RESPONSES TO ACUTE ETHANOL INTOXICATION AND STRESS IN TWO POPULATIONS AT HIGH RISK FOR ALCOHOL MISUSE

Significant literature on the differential characteristics of an individual‘s physiological response to alcohol, and moreover on the differential characteristics among individuals who prospectively manifest alcohol use disorders suggests that there are multiple pathways to alcohol misuse. In addition, considerable research has implicated certain personality traits that convey an increased risk for alcohol misuse. The SURPS questionnaire identifies these high-risk individuals based on four personality traits that are significantly related to the psychobiological motivation systems that mediate an individual‘s response to alcohol. Two systems in particular, the neuroendocrine and cardiovascular, have been identified for their impact on an individual‘s response to acute alcohol intoxication, and furthermore for their contribution in their use as biological predictors to one‘s risk for future alcohol and substance abuse disorders. Previous research has implicated HPA axis dysregulation as a marker of clinical utility to predict later alcohol and substance abuse problems. Similarly, the cardiac response to alcohol administration among subsets of individuals has allowed for the identification of a physiological marker in high-risk groups who have a significantly higher post-ethanol heart rate response in contrast with all other groups. Additionally, it has been found that a reinforcing consequence of alcohol intoxication in high-risk men is the stress dampening response, where their cardiovascular reactivity to an unavoidable shock is reduced at moderate to high doses of alcohol.
High prevalence of metabolic syndrome in populations at high and low cardiovascular risk in Bulgaria

Objective: Metabolic syndrome (MetS) is associated with cardiovascular risk. We examined the prevalence of MetS in well -defined groups with manifested cardiovascular disease (CVD) or at high CVD risk, and in a low -risk population. We also analysed the CVD burden of th e MetS in these populations. Methods: A total of 2123 subjects were examined: 1.with history of acute myocardial infarction (AMI) (n=372); 2.with hypertension (n=620); 3.with type 2 diabetes mellitus (n=556) and 4.healthy controls (n=575). All participants underwent standardised examination of cardiovascular risk. Results: The prevalence of the MetS was: 80% in men and 89% in women according to the Adult Treatment Panel III (ATPIII) definition, 85% in men and 93% in women according to the International Diabetes Federation (IDF) definition in the AMI group; 43% in men and 53% in women (ATPIII criteria), 47% in men and 61% in women (IDF criteria) in the hypertensive group; 70% in men and 79% in women (ATPIII), 77% in men and 86% in women (IDF) in the diabetic patients; and 23% in men and women (ATPIII), 30% in men and 36% in women (IDF criteria) in the controls. In logistic regression analysis the presence of MetS was found an independent determinant of previous AMI in the examined population. Conclusion: Excessively high prevalence of MetS was found in populations with or at high CVD risk, and a high prevalence even in a low -risk group in Bulgaria. The presence of MetS increased CVD risk.
Letters to the Editor

I read the article, by Leechavengvongs et al. (2001) with interest. I am surprised that isolated injury to a major branch of the posterior interosseous nerve has been reported only once before in the English literature. Such injuries are not particularly uncommon. I have treated at least two cases during the last 5 years. I would agree that the diagnosis may be missed on initial presentation or even at operation if the surgeon is not familiar with the anatomy of the posterior interosseous nerve. Late exploration is difficult as a result of scarring and damage to intact nerve branches is a definite risk. The results of repair of the posterior interosseous nerve, as with the terminal motor branches of other nerves, are usually good (Shergill et al., 2001). I note that sural nerve was used for the nerve grafts, which were only 2 cm in length. Short lengths of nerve graft can be obtained in the forearm from the lateral cutaneous nerve of the forearm or the terminal branch of the posterior interosseous nerve (sensory branch to the wrist joint). Such nerve grafts may often be harvested through the same incision and avoid the use of the sural nerve which can provide up to 40 cm of graft.
Infrared thermography as a non-invasive tool to study animal welfare

Growing public concern regarding animal welfare and consumer demand for humanely produced products have placed pressure on the meat, wool and dairy industries to improve and confirm the welfare status of their animals. This has increased the need for reliable methods of assessing animal welfare during commercial farm practices. The measurement of the stress caused by commercial farm practices is a major component of animal welfare assessment. However, a major issue for animal welfare science is that many of the techniques used to measure stress involve invasive procedures, such as blood sampling, which may themselves cause a stress response and therefore affect the measurement of interest. To reduce this problem, a number of non-invasive or minimally invasive methods and devices have been developed to measure stress. These include the measurement of cortisol concentrations in saliva and faeces, and remote devices for recording body temperature, heart rate and the collection of blood samples. This review describes the benefits and limitations of some of these methods for measuring stress. In particular, the review focuses on recent advances and current research in the use of infrared thermography (IRT) for measuring stress. Specific applications for IRT in the dairy and beef industries are also described including an automated, non-invasive system for early diagnosis of infection in cattle. It is essential that non-invasive measures of acute and chronic stress are developed for reliable assessment of animal welfare during standard farm management practices and IRT may be a useful tool for this purpose. IRT may offer advantages over many other non-invasive systems as it appears to be capable of measuring different components of the stress axis, including acute sympathetic and hypothalamic-pituitary-adrenocortical responses.
Using Clicker Technology with Rural, Low-Income Mothers: Collecting Sensitive Data Anonymously

As part of a multi-state study on health message development, a group of family researchers, Extension faculty, and a learning technologist used audience-response systems, or "clickers," to display and record focus group participants' responses to questions. This article describes how the authors used clicker technology in focus group settings, clicker training for facilitators, and lessons learned. The clicker technique is useful to collect local and personal data anonymously in group settings for program evaluation purposes. Implications for future research and Extension education are discussed.
Clinical Data Analysis with the Use of Artificial Neural Networks of Treatment Evaluation in Adolescent Idiopathic Scoliosis Analiza danych klinicznych za pomocą sztucznych sieci neuronowych podczas oceny leczenia skoliozy idiopatycznej

Background. An advanced multivariate data processing method, artificial neural network (ANN) analysis, was demonstrated to provide an objective evaluation of medical strategy by making use of large collections of routine clinical parameters of patients with adolescent idiopathic scoliosis. The ANN method allowed for detection of com− plex systematic relationships between diverse patient, disease and treatment variables. Material and Methods. Two hundred ninety−three patients with adolescent idiopathic scoliosis were the subjects of the study for whose fifteen disease and treatment variables were determined. Results. It was revealed by ANN that it is possible to perform the correct classification of patients regarding dete− rioration, stabilization or improvement of their health observed after four years of treatment. Conclusions. ANN has been recommended as a new promising alternative to classical regression analysis of mul− tivariable clinical data. By means of ANN practically useful systematic information may be extracted from large sets of data otherwise hardly interpretable in comprehensive physical terms. That information can be valuable for general prognosis and appropriate adjustment of the treatment (Adv Clin Exp Med 2004, 13, 4, 623–629).
Using Electronic Step Counters to Increase Lifestyle Physical Activity: Colorado on the Move™

Background: Obesity is an epidemic in the United States, yet few programs have been implemented on a widespread basis to deal with it. Colorado on the Move is a state-wide program with a specific quantifiable behavioral goal for increasing lifestyle physical activity (i.e. walking) and decreasing energy intake to prevent weight gain. Methods: A nonrandomized intervention trial designed to increase walking by 2000 steps/d using electronic step counters. Results: The intervention was effective in increasing average steps/d by at least 2000 over a 14-wk period. Conclusion: The Colorado on the Move intervention was effective in significantly increasing physical activity over a 14-wk period. Steps/d appears to be a good target for use in interventions to increase physical activity. Simply increasing physical activity in the population by 2000 steps/d could help in preventing the average yearly increase in body weight seen in the US population.
Draft Genome Sequence of Enterobacter hormaechei ENT5, a Component of the Symbiotic Community of Tephritid Flies

Enterobacter strains are among the dominant symbiotic bacteria in the gastrointestinal tract of insects, with the ability to fulfill diverse roles. In this announcement, we describe the draft genome sequence of Enterobacter hormaechei strain ENT5, isolated from wild adult Zeugodacus cucurbitae flies. ABSTRACT Enterobacter strains are among the dominant symbiotic bacteria in the gastrointestinal tract of insects, with the ability to fulfill diverse roles. In this announcement, we describe the draft genome sequence of Enterobacter hormaechei strain ENT5, isolated from wild adult Zeugodacus cucurbitae flies.
Hydrogel co-networks of gelatine methacrylate and poly(ethylene glycol) diacrylate sustain 3D functional in vitro models of intestinal mucosa

Mounting evidence supports the importance of the intestinal epithelial barrier and its permeability both in physiological and pathological conditions. Conventional in vitro models to evaluate intestinal permeability rely on the formation of tightly packed epithelial monolayers grown on hard substrates. These two-dimensional models lack the cellular and mechanical components of the non-epithelial compartment of the intestinal barrier, the stroma, which are key contributors to the barrier permeability in vivo. Thus, advanced in vitro models approaching the in vivo tissue composition are fundamental to improve precision in drug absorption predictions, to provide a better understanding of the intestinal biology, and to faithfully represent related diseases. Here, we generate photo-crosslinked gelatine methacrylate (GelMA)—poly(ethylene glycol) diacrylate (PEGDA) hydrogel co-networks that provide the required mechanical and biochemical features to mimic both the epithelial and stromal compartments of the intestinal mucosa, i.e. they are soft, cell adhesive and cell-loading friendly, and suitable for long-term culturing. We show that fibroblasts can be embedded in the GelMA-PEGDA hydrogels while epithelial cells can grow on top to form a mature epithelial monolayer that exhibits barrier properties which closely mimic those of the intestinal barrier in vivo, as shown by the physiologically relevant transepithelial electrical resistance (TEER) and permeability values. The presence of fibroblasts in the artificial stroma compartment accelerates the formation of the epithelial monolayer and boosts the recovery of the epithelial integrity upon temporary barrier disruption, demonstrating that our system is capable of successfully reproducing the interaction between different cellular compartments. As such, our hydrogel co-networks offer a technologically simple yet sophisticated approach to produce functional three-dimensional (3D) in vitro models of epithelial barriers with epithelial and stromal cells arranged in a spatially relevant manner and near-physiological functionality.
Complete Genome Sequence of vB_EcoP_PR_Kaz2018, a T7-Like Bacteriophage

Here, we describe the complete genome sequence of the Escherichia coli bacteriophage vB_EcoP_PR_Kaz2018, isolated from a water sample. vB_EcoP_PR_Kaz2018 is a linear double-stranded DNA T7-like podophage with a genome of 39,704 bp containing 45 predicted open reading frames (ORFs). ABSTRACT Here, we describe the complete genome sequence of the Escherichia coli bacteriophage vB_EcoP_PR_Kaz2018, isolated from a water sample. vB_EcoP_PR_Kaz2018 is a linear double-stranded DNA T7-like podophage with a genome of 39,704 bp containing 45 predicted open reading frames (ORFs).
Influence of Dietary Astaxanthin on the Hepatic Oxidative Stress Response Caused by Episodic Hyperoxia in Rainbow Trout

A 13-week feeding trial was carried out with juvenile rainbow trout to test two diets: a control diet without astaxanthin (AX) supplementation (CTRL diet), and a diet supplemented with 100 mg/kg of synthetic AX (ASTA diet). During the last week of the feeding trial, fish were exposed to episodic hyperoxia challenge for 8 consecutive hours per day. Episodic hyperoxia induced physiological stress responses characterized by a significant increase in plasma cortisol and hepatic glycogen and a decrease in plasma glucose levels. The decrease of plasma glucose and the increase of hepatic glycogen content due to episodic hyperoxia were emphasized with the ASTA diet. Hyperoxia led to an increase in thiobarbituric acid-reactive substances in the muscle, diminished by dietary AX supplementation in both liver and muscle. Muscle and liver AX were increased and decreased respectively after 7-day episodic hyperoxia, leading to an increase in flesh redness. This augment of muscle AX could not be attributed to AX mobilization, since plasma AX was not affected by hyperoxia. Moreover, hyperoxia decreased most of antioxidant enzyme activities in liver, whereas dietary AX supplementation specifically increased glutathione reductase activity. A higher mRNA level of hepatic glutathione reductase, thioredoxin reductase, and glutamate-cysteine ligase in trout fed the ASTA diet suggests the role of AX in glutathione and thioredoxin recycling and in de novo glutathione synthesis. Indeed, dietary AX supplementation improved the ratio between reduced and oxidized glutathione (GSH/GSSG) in liver. In addition, the ASTA diet up-regulated glucokinase and glucose-6-phosphate dehydrogenase mRNA level in the liver, signaling that dietary AX supplementation may also stimulate the oxidative phase of the pentose phosphate pathway that produces NADPH, which provides reducing power that counteracts oxidative stress. The present results provide a broader understanding of the mechanisms by which dietary AX is involved in the reduction of oxidative status.
Systematic Review of the Cost Effectiveness of Breast Cancer Prevention, Screening, and Treatment Interventions.

It has been nearly 5 decades since Congress passed the 1971 National Cancer Act to strengthen the National Cancer Institute’s ability to eliminate the suffering from cancer. Most early efforts of the National Cancer Institute focused on development of cancer therapeutics. Later, Congress passed the 21st Century Cancer Access to Life-Saving Early Detection, Research and Treatment Act to increase the national research focus on prevention and early detection.
Dynamic Changes in the Localization of Neuronatin-Positive Cells during Neurogenesis in the Embryonic Rat Brain

Neuronatin (NNAT) was first identified as a gene selectively and abundantly expressed in the cytoplasm of the newborn mouse brain, and involved in neonatal neurogenesis. However, the particular roles of NNAT in the developing prenatal brain have not been identified, especially in mid to late stages. In this study, we performed immunohistochemical analyses of NNAT and SOX2 proteins, a nuclear transcription factor and neural stem/progenitor marker, in the rat brain on embryonic days 13.5, E16.5, and E20.5. NNAT signals were broadly observed across the developing brain on E13.5 and gradually more localized in later stages, eventually concentrated in the alar and basal parts of the terminal hypothalamus, the alar plate of prosomere 2 of the thalamus, and the choroid plexus in the lateral and fourth ventricles on E20.5. In particular, the mammillary body in the basal part of the terminal hypothalamus, a region with a high number of SOX2-positive cells, evidenced intense NNAT signals on E20.5. The intracellular localization of NNAT showed diverse profiles, suggesting that NNAT was involved in various cellular functions, such as cell differentiation and functional maintenance, during prenatal neurogenesis in the rat brain. Thus, the present observations suggested diverse and active roles of the NNAT protein in neurogenesis. Determining the function of this molecule may assist in the elucidation of the mechanisms involved in brain development.