Miracle-dz/UniFAR-FTUs huggingface dataset:
Running head: Rapid cloning of tissue-specific inducible lines

Tissue-specific inducible system that allows combination of inducible promoters, genes, reporters and plant selection markers in a single cloning step ABSTRACT A powerful method to study gene function is expression or overexpression in an inducible, 48 cell type-specific system followed by observation of consequent phenotypic changes and 49 visualization of linked reporters in the target tissue. Multiple inducible gene overexpression 50 systems have been developed for plants, but very few of these combine plant selection 51 markers, control of expression domains, access to multiple promoters and protein fusion 52 reporters, chemical induction, and high-throughput cloning capabilities. Here, we introduce a 53 MultiSite Gateway ® compatible inducible system for plants that provides the capability to 54 generate such constructs in a single cloning step. The system is based on the tightly 55 controlled, estrogen-inducible XVE system. We demonstrate that the transformants 56 generated with this system exhibit expected cell-type specific expression, similar to what is 57 observed with constitutively-expressed native promoters. With this new system, cloning of 58 inducible constructs is no longer limited to a few special cases, but can be used as standard 59 approach when gene function is studied. In addition, we present a set of entry clones 60 consisting of histochemical and fluorescent reporter variants designed for gene and 61 promoter expression studies. temporal vessel differentiation young Arabidopsis seedlings expression of an immature element-specific
Early Prediction of Gestational Diabetes in Vietnam Clinical impact of currently recommended diagnostic criteria

OBJECTIVES d Weaimedtocompare the discriminativepower of prognosticmodels for early prediction of women at risk for the development of gestational diabetes mellitus (GDM) using four currently recommended diagnostic criteria based on the 75-g oral glucose tolerance test (OGTT). We also described the potential effect of application of the models into clinical practice. RESEARCH DESIGN AND METHODS d A prospective cross-sectional study of 2,772 pregnant women was conducted at a referral maternity center in Vietnam. GDM was determined by the American Diabetes Association (ADA), International Association of Diabetes and Pregnancy Special Group (IADPSG), Australasian Diabetes in Pregnancy Society (ADIPS), and World Health Organization (WHO) criteria. Prognostic models were developed using the Bayesian model averaging approach, and discriminative power was assessed by area under the curve. Different thresholds of predicted risk of developing GDM were applied to describe the clinical impact of the diagnostic criteria. RESULTS varied substantially by diagnostic criteria:
Textual Query Based Image Retrieval

-As digital cameras becoming popular and mobile phones are increased very fast so that consumers photos are increased. So that retrieving the appropriate image depending on content or text based image retrieval techniques has become very vast. Content-based image retrieval, a technique which uses visual contents to search images from large scale image databases according to users interests, has been an active and fast advancing research area semantic gap between the low-level visual features and the high-level semantic concepts. Real-time textual query-based personal photo retrieval system by leveraging millions of Web images and their associated rich textual descriptions. Then user provides a textual query. Our system generates the inverted file to automatically find the positive Web images that are related to the textual query as well as the negative Web images that are irrelevant to the textual query. For that purpose we use k-Nearest Neighbor (kNN), Decision stumps, and linear SVM, to rank personal photos. For improvement of the photo retrieval performance, we have used two relevance feedback methods via cross-domain learning, which effectively utilize both the Web images and personal images.
Comprehensive identi cation and characterization of HERV-K (HML-9) group in the human genome

Lin Li (  dearwood@sina.com ) Beijing Institute of Microbiology and Epidemiology Lei Jia Beijing Institute of Microbiology and Epidemiology Mengying Liu Beijing University of Chemical Technology Hanping Li Beijing Institute of Microbiology and Epidemiology Yongjian Liu Beijing Institute of Microbiology and Epidemiology Jingwan Han Beijing Institute of Microbiology and Epidemiology Xiuli Zhai Beijing Institute of Microbiology and Epidemiology Xiaolin Wang Beijing Institute of Microbiology and Epidemiology Tianyi Li Beijing Institute of Microbiology and Epidemiology Jingyun Li Beijing Institute of Microbiology and Epidemiology Bohan Zhang Beijing Institute of Microbiology and Epidemiology Changyuan Yu Beijing University of Chemical Technology
Knowledge Graph-Based Recipe Recommendation System

Recommendation systems are in many and diverse end-user applications, like e-commerce services, online audio or video-sharing platforms as well as media-services and web-content providers. A recommendation system is composed of software tools and techniques whose primary goal is to provide suggestions for items or services a user may wish to utilise. Regarding the methodology used, recommendation systems are generally categorised as contentbased filtering, if the recommendation uses the characteristics of items; collaborative filtering, if a recommendation resorts to the preferences of similar users; and hybrid recommendation, if a recommendation combines the two previous techniques. Most of the existing recommendation systems use a hybrid approach on the culinary’s domain, mixing collaborative filtering and content filtering, focusing on the recipe and the user’s rating, behaviours, history, reviews, and preferences. However, culinary’s recipes are items formed by heterogeneous information whose content has high value for the user. Good examples are the type of ingredients, ingredient’s quantities, cooking procedure, number of steps or recipe’s categories. Recent research concludes that models can be improved in accuracy and diversity if the recommendation system considers an item as a composition of characteristics, instead of a singular item approach. The paper presents an implementation and results of a recipe recommendation system within the culinary’s domain, which recognises a recipe as a composition of potential features organised in a structure such as a knowledge graph. This endeavour’s resource is a dataset obtained from Food website with recipes information and item’s explicit rating given by the user. The methodology consists of results comparison between the most common approaches in recommendation systems and knowledge graph-based convolutional network. Graph convolutional network is the only one in our research which produces a model based on a knowledge graph constructed. The experimental results demonstrate an improvement in rating prediction accuracy when using a recommendation system’s knowledge graph.
Adenine Deaminase and Adenine Utilization in Saccharomyces cerevisiae

Compared with other purine salvage and nitrogen catabolism enzymatic activities, adenine deaminase (adenine aminohydrolase [AAHJ; EC 3.5.4.2) activity in Saccharomyces cerevisiae is uniquely regulated. AAH specific activity is not induced by adenine and is reduced sevenfold when cells are cultivated in medium containing proline in place of ammonium as the sole nitrogen source. Exogenous adenine enters metabolic pathways primarily via the function of either AAH or adenine phosphoribosyltransferase (APRT; EC 2.4.2.7). Exogenous adenosine cannot normally be utilized as a purine source. Strains efficiently utilized adenosine or inosine when grown in pH 4.5 medium containing Triton X-100. A recessive mutation permitting utilization of adenosine or inosine in standard media was isolated. In both situations, growth of purine auxotrophs required either AAH or APRT activity. With medium containing either ammonium or proline as a nitrogen source, minimum doubling times of purine auxotrophs deficient in either APRT or AAH were measured. In proline-based medium, AAH and APRT permitted equal utilization of exogenous adenine. In ammonium-based medium, the absence of APRT increased the minimum doubling time by 50%. Similar experiments using sufficient exogenous histidine to feedback inhibit histidine biosynthesis failed to affect the growth rates of adenine auxotrophs blocked in AAH or APRT, indicating that the histidine-biosynthetic pathway does not play a significant role in adenine utilization. The gene that encodes AAH in S. cerevisiae was isolated by complementation using yeast strain XD1-1, which is deficient in AAH, APRT, and purine synthesis. A 1.36-kb EcoRI-SphI fragment was demonstrated to contain the structural gene for AAH by expressing this DNA in Escherichia coli under control of the trp promoter-operator. Northern (RNA) studies using the AAH-, APRT-, and CDC4-coding regions indicated that AAH regulation was not mediated at the level of transcription or mRNA degradation.
Wind or Hydro Homo-Heteropolar Synchronous Generators: Equivalent Magnetic Circuit and FEM Analysis

In an effort to introduce a low cost (PM less), low power electric wind or hydro generators, this paper reports on preliminary design aspects, equivalent magnetic circuit and 3D FEM analysis of a 2.5 KVA, 250-1000 rpm, reactive homo-heteropolar brushless synchronous machine (RHHBSM).
Deep Learning-Based Corn Disease Tracking Using RTK Geolocated UAS Imagery

Deep learning-based solutions for precision agriculture have achieved promising results in recent times. Deep learning has been used to accurately classify different disease types and disease severity estimation as an initial stage for developing robust disease management systems. However, tracking the spread of diseases, identifying disease hot spots within cornfields, and notifying farmers using deep learning and UAS imagery remains a critical research gap. Therefore, in this study, high resolution, Unmanned Aerial System (UAS) acquired, Real-Time Kinematic (RTK) geotagged, RGB imagery at heights of 3 meters and 12 meters above ground level (AGL), was used to track disease hot spots in corn fields throughout the growing season. A total of 98,000 RGB images with a resolution of 8192 x 5460 pixels were acquired in cornfields located at Purdue University’s Agronomy Center for Research and Education (ACRE), using a DJI Matrice 300 with an RTK base station mounted with a 45-megapixel DJI Zenmuse P1 camera, from June 28th to August 31st, 2021. After carefully selecting images acquired at one-week
DEV104018 1..9

In the adultDrosophilamidgut the bonemorphogenetic protein (BMP) signaling pathway is required to specify and maintain the acidsecreting region of the midgut known as the copper cell region (CCR). BMP signaling is also involved in themodulation of intestinal stem cell (ISC) proliferation in response to injury. How ISCs are able to respond to the same signaling pathway in a regionally different manner is currently unknown. Here, we show that dual use of the BMP signaling pathway in the midgut is possible because BMP signals are only capable of transforming ISC and enterocyte identity during a defined window of metamorphosis. ISC heterogeneity is established prior to adulthood and then maintained in cooperation with regional signals from surrounding tissue. Our data provide a conceptual framework for how other tissues maintained by regional stem cells might be patterned and establishes the pupal and adult midgut as a novel genetic platform for identifying genes necessary for regional stem cell specification and maintenance.
DNA Binding and Uptake by Nuclei Isolated from Plant Protoplasts FATE OF SINGLE-STRANDED BACTERIOPHAGE fd DNA1

Binding and uptake of exogenous DNA by nuclei isolated from Glycine max L. Meff were studied using 3H-labeled single-stranded DNA of bacteriophage fd. A comparison of single-stranded with double-stranded DNA for binding and uptake by nuclei was also made. Isolated nuclei were incubated with 3H-labeled single-stranded bacteriophage fd DNA. Poly-L-lysine or DEAE-dextran at 0.1 and 1 pg/ml stimulated DNA binding. On the other hand, poly-L-lysine at 1 and 5 pg/ml increased DNA uptake but DEAE-dextran did not. Ten to 20 mm Ca and Mg ions were required for DNA uptake. EDTA (1-20 mM) did not differ from the control or the low levels ofCa or Mg ions. These observations on single-stranded fd DNA differed from those obtained with double-stranded DNA of SalmoneHla typhimurium. Kinetics of single-stranded DNA binding and uptake also deviated from that of double-stranded DNA. Analyses of nuclei-bound DNA by sucrose density gradient and CsCI density gradient centrifugation revealed extensive DNA degradation during a 45-minute incubation period. Poly-L-lysine protected against rapid deg- radation ofbound DNA. DEAE-dextran enhanced DNA binding, but bound DNA was cleaved into much smaller polymers than those detected in control experiments or in the presence of poly-L-lysine. Sucrose density gradient and CsCI density gradient centrifugation analyses on DNA taken up by nuclei also showed extensive DNA degradation. Poly-L-lysine slightly inhibited DNA degradation, but DEAE-dextran appeared to enhance deg- radatdon of incorporated DNA. Moreover, nonbound DNA in the
NDT_A_317803 2849..2857

Department of Psychiatry, Dilla University’s College of Medicine and Health Sciences, Dilla, Ethiopia Background: Returning internally displaced persons (IDPs) to their homes can be a difficult and traumatic experience, particularly after a long period of displacement. Furthermore, traumatic experiences encountered during displacement during the flight process may be replayed in the minds of IDP returnees when they return to their original locations, raising the risk of re-traumatization. This could have a detrimental impact on the mental health of returning IDPs. Despite this, the mental health of returnee IDPs in Ethiopia has received less consideration. As a result, the aim of this study was to determine the prevalence and determinants of mental distress among Ethiopian returnees. Method and Participants: A community-based cross-sectional study with 623 participants was conducted from January 1 to 30, 2021, using a systematic sampling technique. The Kessler psychological distress scale was used to assess mental distress (K-10). Bivariable and multivariable binary logistic regression is used to investigate the relationship between variables. Statistical significance was described as a P-value of less than 0.05 with a 95% confidence interval. Results: The total number of people interviewed was 623, with a 100% response rate. With a 95% confidence interval of 47 to 51.9, the prevalence of psychological distress was 49.4%. Unemployment (AOR=2.1, 95% CI 1.38 to 3.40), inadequate social support (AOR=1.92, 95% CI 1.53 to 2.56), having little knowledge of post-returnee life (AOR=2.17, 95% CI 1.23 to 3.37), and the number of traumatic events (AOR=1.92, 95% CI 1.53 to 2.56) were all found to be significant in the multivariate logistic regression. Conclusion: Returnee IDPs have a high prevalence of psychological distress, according to this report. It is suggested that knowledge of how to adapt to a new life after returning to the original land be given, as well as consistent psychosocial help.
The Impact of a Lack of Government Strategies for Sustainable Water Management and Land Use Planning on the Hydrology of Water Bodies: Lessons Learned from the Disappearance of the Aculeo Lagoon in Central Chile

Several studies have focused on why the Aculeo Lagoon in central Chile disappeared, with a recent one concluding that a lack of precipitation was the main cause, bringing tremendous political consequences as it supported the argument that the government is not responsible for this environmental, economic, and social disaster. In this study, we evaluated in detail the socio-economic history of the watershed, the past climate and its effects on the lagoon’s water levels (including precipitation recycling effects), anthropogenic modifications to the lagoon’s water balance, the evolution of water rights and demands, and inaccurate estimates of sustainable groundwater extraction volumes from regional aquifers. This analysis has revealed novel and undisputable evidence that this natural body of water disappeared primarily because of anthropogenic factors (mostly river deviations and aquifer pumping) that, combined with the effects of less than a decade with below-normal precipitation, had a severe impact on this natural lagoon–aquifer system.
Towards a General Framework for

Recent studies reveal that there are different methodologies for developing Intelligent Environments. Thus, it has become essential to scrutinize and evaluate the methodologies to increase our understanding of their strengths, weaknesses and features. However, these concerns have not been the target of recent research efforts. This paper presents an evaluation framework for qualitative evaluation of Intelligent Environment methodologies. It is a step towards standardization of current Intelligent Environments methodologies. The framework has been defined through studying, abstracting and unifying best practices from systems engineering. It is based on a generic life cycle model. As an initial validation, we evaluated the User Centred Intelligent Environment Development Process against the proposed framework. We note that this methodology at its current state presents some limitations which will be addressed in future works.
Modelisation et mesure des proprietes radioelectriques de materiaux heterogenes en ligne de transmission

Des perturbations dues a la resonance de modes d'ordre superieur dans un echantillon peuvent apparaitre sur les courbes de mesure lorsque l'on caracterise des materiaux heterogenes en espace guide. Une technique de thermographie infrarouge est utilisee pour observer l'evolution du champ electromagnetique dans la cellule de mesure. D'une part une interpretation physique basee sur la theorie des resonateurs dielectriques est donnee. D'autre part un modele, a base de resonateurs rlc, permettant d'exploiter les mesures est introduit
Is the Continuance a New Buzz Word? Bibliometric and Co-citation Analysis of E-learning Continuance

– Through the bibliometric and co-citation analysis, this paper offers a review of science achievements in the area of e-learning continuance, with focus on identification of the field relevance, authors and most cited and co-cited papers, as well as the identification of roots this research area is based on. Based on co-citation network, which chronologically starts with Ajzen’s theory of 1975, another contribution can be drawn from this paper. That contribution leads to a conclusion that Ajzen’s Theory of planned behavior is a starting and adequate basis for all identified research and creates a main recommendation for upcoming researches.
Assay of Memantine Hydrochloride by UV Spectrophotometer

- Memantine hydrochloride is a class of N-Methyl-D-Aspartase receptor antagonist used in the treatment of Alzheimer’s disease. The main aim of the present study was to develop a method to determine Memantine hydrochloride in tablet formulation which is simple, sensitive, precise and economical as per ICH guidelines. The method was validated at λ max of 254nm. Beer-Lambert’s was obeyed between the concentration ranges of 0.2-0. 6µg/ml. A good linear relationship with correlation coefficient of 0.998 was obtained and the LOD and LOQ values were 0. 48µg/ml and 1. 48µg/ml respectively. The method was also employed on the tablet formulation (Namenda) and the recovery was found to be 99.8%.
Charge pump phase-locked loop with phase-frequency detector: closed form mathematical model

Charge pump phase-locked loop with phase-frequency detector (CP-PLL) is an electrical circuit, widely used in digital systems for frequency synthesis and synchronization of the clock signals. In this paper a non-linear second-order model of CP-PLL is rigorously derived. The obtained model obviates the shortcomings of previously known second-order models of CP-PLL. Pull-in time is estimated for the obtained second-order CP-PLL.
Bahasa Malaysia Version of Urogenital Distress Inventory (UDI-6) and Incontinence Impact Quality of Life Questionnaires (IIQ-7), a Cross Sectional Study

Background : Urinary incontinence (UI) demonstrates major prevalence in women of different population groups. Reduced quality of life (QOL) is observed due to incontinence problems. Urogenital Distress Inventory (UDI-6) and Incontinence Impact Quality of Life (IIQ-7) are useful disease-specific questionnaires evaluating the impact of urinary incontinence on the QOL of women which is accepted internationally. Objective : This study aims to translate and validate UDI-6 and IIQ-7 in Malay language. Methods: A cross sectional study, which recruited 100 participants from two urogynecology clinics. Both questionnaires were initially translated from English to Bahasa Malaysia followed by back translation and final correction done by the professional translators. The participants were requested to maintain a urinary record of the upcoming week for three days that assisted in quantifying the severity of symptoms. None of the subjects were assigned any treatment during the study period. Validity and reliability of the translated questionnaires were determined by checking the internal consistency and also by doing test-retest. Results: The internal consistency levels of the UDI-6 and IIQ-7 Bahasa Malaysia questionnaires were 0.73 and 0.90 respectively with good test-retest (0.86 and 0.95). Incontinence episodes were strongly associated with obstructive, irritative, and stress symptoms. The factor of day time voiding had strong correlation with obstructive and irritative symptoms. Conclusion: UDI-6 and IIQ-7 did not measure similar outcomes; however, both questionnaires have their strengths in clinical settings. Analysis has also revealed that the Malaysian versions of both questionnaires had appropriate test-retest validity and reliability. Thus, it can be said that both of the questionnaires had great importance for screening patients with urinary incontinence in Malaysia.
Fourth Human ParechovirusSerotype

We identified a novel human parechovirus (HPeV) type (K251176-02) from a neonate with fever. Analysis of the complete genome showed K251176-02 to be a new HPeV genotype. Since K251176-02 could not be neutralized with antibodies against known HPeV serotypes 1–3, it should be classified as a fourth HPeV serotype.