Miracle-dz/UniFAR-FTUs huggingface dataset:
Urethral Bulking in the Treatment of Stress and Mixed Female Urinary Incontinence: Results from a Multicenter Cohort and Predictors of Clinical Outcomes

The aim of the present study is to analyze the outcomes of urethral bulking in the treatment of non-neurogenic female stress and mixed urinary incontinence and to assess predictors of clinical outcomes. We retrospectively included all consecutive patients affected by stress or mixed urinary incontinence and treated with urethral bulking. Outcomes were evaluated via the PGI-I questionnaire and the 24-h pad test. Between January 2010 and January 2020, we treated 216 patients (Bulkamid n = 206; Macro-plastique n = 10). The median age at surgery was 66 years (IQR 55–73.75). The median follow-up was 12 months (IQR 12–24). In total, 23.8% of patients were subjected to prior incontinence surgery, 63.8% of patients were affected by genuine stress urinary incontinence, 36.2% reported mixed urinary incontinence, whereas detrusor overactivity was confirmed in only 24.9%. The dry rate was 32.9%; nevertheless, 69.9% of patients declared themselves “very improved” or “improved” (PGI-I1-2). Low complications were observed, mostly classified as Clavien I. After univariate and multivariate analyses, the only statistically significant independent predictor of “dry” outcome was the 24 h pad test, p < 0.001. Urethral bulking could be proposed with more expectations of success in patients with mild urinary incontinence. Patients affected by moderate–severe incontinence are less likely to obtain clinical success; therefore, they should be carefully counselled about clinical expectations before the procedure.
The impact of quality control in RNA-seq experiments

High throughput mRNA sample sequencing, known as RNA-seq, is as a powerful approach to detect differentially expressed genes starting from millions of short sequence reads. Although several workflows have been proposed to analyze RNA-seq data, the experiment quality control as a whole is not usually considered, thus potentially biasing the results and/or causing information lost. Experiment quality control refers to the analysis of the experiment as a whole, prior to any analysis. It not only inspects the presence of technical effects, but also if general biological assumptions are fulfilled. In this sense, multivariate approaches are crucial for this task. Here, a multivariate approach for quality control in RNA-seq experiments is proposed. This approach uses simple and yet effective well-known statistical methodologies. In particular, Principal Component Analysis was successfully applied over real data to detect and remove outlier samples. In addition, traditional multivariate exploration tools were applied in order to asses several controls that can help to ensure the results quality. Based on differential expression and functional enrichment analysis, here is demonstrated that the information retrieval is significantly enhanced through experiment quality control. Results show that the proposed multivariate approach increases the information obtained from RNA-seq data after outlier samples removal.
Exploratory Systematic Review and Meta-Analysis of Panax Genus Plant Ingestion Evaluation in Exercise Endurance

Background: Many studies that use food containing Panax genus plants (PGPs) have been conducted but most of them have not mentioned the effective compounds ginsenosides and their composition. Therefore, we conducted a systematic review and meta-analysis of time to exhaustion as an index of exercise endurance with ingestion of PGPs or ginsenosides to reveal their effects. Methods: We performed a systematic review with a comprehensive and structured literature search using seven literature databases, four clinical trial databases, and three general web search engines during 15–22 March 2021. A random-effects model was applied to calculate the standardized mean difference (SMD) and 95% confidence interval (CI) as the difference between the mean in the treatment and placebo groups. We evaluated the risk of bias of individual studies along with the risk of bias tool in the Cochrane handbook. This study was funded by Maruzen Pharmaceuticals Co., Ltd. (Hiroshima, Japan). The protocol for this study was registered with the UMIN-CTR (No. UMIN000043341). Results: Five studies met the inclusion criteria. The number of total participants was 90, with 59 in the ingestion-PGPs group and 64 in the control group, because three studies were crossover-design trials. We found that ingestion of PGPs or ginsenosides significantly improved exercise endurance (SMD [95% CI]: 0.58 [0.22–0.95], I2 = 0%). It was suggested that ginsenoside Rg1 (Rg1) and PGPs extract containing Rg1 were significantly effective in improving exercise endurance (SMD [95% CI]: 0.70 [0.14–1.27], I2 = 30%) by additional analysis. Conclusions: This systematic review suggests that the ingestion of PGPs or ginsenosides, especially Rg1, is effective in improving exercise endurance in healthy adults. However, further high-quality randomized controlled trials are required because imprecision and publication bias cannot be ignored in this systematic review.
Independent and Combined Association of Lifestyle Behaviours and Physical Fitness with Body Weight Status in Schoolchildren

(1) Background: Lifestyle behaviours and physical fitness play a critical role in the development of childhood obesity. It has been demonstrated in this study that self-reported physical fitness is representative of a healthy lifestyle and thus is associated with a lower incidence of overweight/obesity. The objective of this study was to analyse the independent and combined association of lifestyles (physical activity, screen time, diet and hours of sleep) and self-reported physical fitness with body weight in schoolchildren. (2) Methods: This study performed a descriptive and cross-sectional analysis. The study sample consisted of 864 schoolchildren between 8–9 years old from 26 schools of the province of Cádiz. To measure lifestyles and self-reported physical fitness, questionnaires were administered to both schoolchildren and families. To obtain the body weight status, the children were measured by body mass index (BMI). To verify the influence of lifestyles and self-reported physical fitness on the body weight status of schoolchildren, a combined score of lifestyles and self-reported physical fitness was calculated. (3) Results: Schoolchildren who followed healthier lifestyles and presented good physical fitness had a better body weight status (p < 0.001). Schoolchildren who had less healthful lifestyles and bad physical fitness had a 10.34 times higher risk of being overweight or obese (p = 0.004). (4) Conclusions: It has been shown that there is an independent and combined association between lifestyles and physical fitness on the body weight of the schoolchildren. We have suggested strategies to get children to adopt healthy lifestyles and good physical fitness to maintain a healthy body weight and prevent obesity.
English speaking proficiency assessment using speech and electroencephalography signals

In this paper, the English speaking proficiency level of non-native English speakerswas automatically estimated as high, medium, or low performance. For this purpose, the speech of 142 non-native English speakers was recorded and electroencephalography (EEG) signals of 58 of them were recorded while speaking in English. Two systems were proposed for estimating the English proficiency level of the speaker; one used 72 audio features, extracted from speech signals, and the other used 112 features extracted from EEG signals. Multi-class support vector machines (SVM) was used for training and testing both systems using a cross-validation strategy. The speech-based system outperformed the EEG system with 68% accuracy on 60 testing audio recordings, compared with 56% accuracy on 30 testing EEG recordings.
Evaluation of the use of breast cancer screening methods among female patients through Champion Health Belief Model Scale

Aim : The aim of this study is to evaluate the use of breast cancer screening methods by women in our society and its affecting factors. Material and Method: A total of 281 women aged 40 years and older were included in the study. A sociodemographic data form, Breast Cancer Risk Assessment Form and the Champion’s Health Belief Model Scale were applied to the participants through face-to-face interviews. Results : It was found that most participants knew breast cancer screening methods but that they used the methods at a low rate. The main reason affecting their use of screening methods was whether there was a complaint. The rate of mammography was found to be significantly higher among those who performed breast self-examination (p=0.011). When the Champion’s Health Belief Model Scale form was examined, it was found that the participants had the highest points from the self-efficacy subscale of breast self-examination, and the lowest points from the perceived susceptibility subscale. Conclusion : It was observed that the use of any screening method and breast cancer risk factors affected health beliefs regarding the use of screening methods.
Leveraging Microtechnology to Study Multicellular Microvascular Systems and Macromolecular Interaction

Leveraging Microtechnology to Study Multicellular Microvascular Systems and Macromolecular Interactions Hesam Parsa Biological systems are large-scale, complex systems comprised of many hierarchical subsystems interacting physico-chemically in a dynamic and coordinated fashion. The complex interactions of subsystems (in micro-scale) lead to the formation of emergent properties (in macro-scale); these are properties that are not visible if individual subsystems are studied. The inherent high-throughput characteristics of microfabrication technology (microtechnology) along with its ability to manipulate biological species at the micro-scale makes it an ideal tool to elucidate the mechanisms leading to the formation of emergent properties at the macro-scale. In this dissertation, by combining microtechnologies with advanced computational algorithms, we demonstrate system-level analysis of biological systems in development and disease. The abundance of high quality molecular and genetic data along with the drastic increase in computational power resulted in considerable progress in genomics, epigenomics and proteomics, but not for the so-called cellomics as we define it here: high-throughput study of single-cell phenotype and heterotypic cell-cell interaction via micromanipulation and bioinformatics analysis. Lack of high-throughput robust experimental tools is the major roadblock to cellomics. Using microtechnologies, in the context of developmental biology we studied vascular tissue morphogenesis (vasculogenesis). Formation of microvessels is of critical significance in development and for vascularizing newly engineered tissues in regenerative medicine. First, we sought to map the heterogeneous morphodynamic behavior of individual clonal cells in the process of capillary-like structure (CLS) formation (Chapter 2 and 3). Then we looked into deciphering the role of extracellular matrix (ECM) mediated mechanical signals in deriving the process of CLS formation (Chapter 4). In the second half of this thesis, we demonstrated the capabilities of microtechnologies and advanced computational algorithms in tackling the challenging problems in disease: global health diagnostics and cancer drug screening. First, we studied the performance of microfluidicbased diagnostic as a large-scale complex system under real-world constraints (Chapter 5). Then, we present the development of two microfluidic-based platforms to study the heterotypic interaction of cells in both a biomimetic in vitro and a realistic in vivo setting. We developed an implantable construct carrying a densely-packed heterogeneous panel of tumor cells. This platform could ultimately be used to test anti-cancer drug efficacy against a large number of genotypes in an in vivo setting (Appendices A and B). Together, these methods provide a powerful suite of tools for high-throughput analysis of biological species at the micro-scale and could potentially unlock the mysteries behind the emergent properties observed at the macro-scale.
Reverse Genomics: Design of Universal Epitope Sets to Isolate All Saccharibacteria Members from the Human Oral Cavity

Microorganisms not yet cultured represent a large proportion of the microbes described to date. Progress in sequencing and metagenomic tools continues to increase microbial diversity without providing information on their physiological and pathophysiological characteristics, such as the recent discovery of enigmatic microbes belonging to Candidate Phyla Radiation (CPR). Reverse genomics is a recent technique allowing co-cultivation of a few CPR members, affiliated to the Saccharibacteria phylum, based on the analysis of their already-available genomes. Here, our aim is to designate a common system capable of cultivating any given taxon of this phylum from human samples. We managed to design, in silico, 11 common epitopes for all Saccharibacteria species recovered from the human oral cavity and which can serve as antigens via bioinformatics analyses. These sequences allow the synthesis of target antibodies, sorting Saccharibacteria spp. by flow cytometry and co-culturing them afterwards with adapted hosts. This epitope set can facilitate the cultivation of CPR in general, which in recent years has been considered a challenge for microbiologists, and subsequently contributes to better studying this new branch on the tree of life.
Effect of Supplementing Different Levels of L-Glutamine on Holstein Calves during Weaning

Weaning stress affects the health and performance of calves. L-glutamine (L-Gln) is commonly used as a functional antioxidant and energy supplement in the body. However, dietary L-Gln supplementation improving weaning stress of calves is unclear. Thus, we aimed to explore the effects of L-Gln (provided by rumen-protected L-Gln) on calves during weaning. Seventy-five Holstein calves (54.0 ± 2.68 kg; 42 ± 2.1 d of age) were assigned to five groups: no supplementation and L-Gln with 1%, 2%, 3%, and 4% dry matter daily intake (DMI) supplementation groups, respectively. The experiment lasted for 28 days (42–70 d of age of calves), and the calves were weaned at 15 d of experiment. DMI and body weekly weight of all calves were recorded. Blood samples of nine healthy calves with similar body weight were collected from each group at 0, 7, 14, 16, 18, 21, and 28 d of experiment for detecting serum L-Gln, glucose, insulin, urea nitrogen, D-lactate, cortisol, haptoglobin, interleukin-8, immunoglobulin (Ig) G, IgA, IgM, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde. At the end of the experiment, six healthy calves with similar body weight from each group were selected for slaughter and morphological analysis of small intestine tissue. The results showed that the L-Gln supplementation in the diets improved the negative effects of sudden weaning in calves. Furthermore, compared to the higher-level L-Gln supple-mentation (3 and 4% of DMI) groups, the dietary lower-level L-Gln supplementation (1 and 2% of DMI) had higher average daily gain, glutathione peroxidase and IgG concentration, and villus height/crypt depth of the duodenum and jejunum, as well as lower cortisol, haptoglobin, and interleukin-8 concentration of weaned calves. These results provided effective reference for relieving the negative effects of calves during weaning.
Picking Losers: How Career Incentives Undermine Industrial Policy in Chinese Cities

Abstract Rapid economic growth in China with considerable market intervention has led to renewed interest in the efficacy of industrial policy. Have industrial policies helped Chinese firms overcome market failures and boosted productivity, or have they distorted markets and undermined creative destruction? This paper explores these questions in the specific context of local implementation by studying the targeting of financial favors and the relationship of such targeting to local political incentives. Using unique and largely unexplored quasi-census firm-level data from annual tax surveys in China from 2007 to 2015, this paper demonstrates that financial favors disproportionately target loss-making, larger, older, and less productive firms. It then argues that short-term career incentives facing local officials explain this sub-optimal targeting, showing that promotion likelihood for city leaders increases with higher levels of ‘excess’ financial favors to firms, particularly when these favors are provided to loss-making firms. Finally, the paper estimates costs to the Chinese economy from misallocated financial support by showing that financial favors are associated with lower levels of firm entry and lower productivity growth. The paper contributes to the literature on industrial policy, political incentives, and misallocation, and has important implications for China’s future growth and transition.
Detection of Mobile Phone Screen Defect Based on Faster R-CNN Fusion Model

With the increasing prevalence of mobile phone products, the quality of mobile phones has become more and more vital, especially the quality of mobile phone screens. Therefore, the accurate detection of screen defects is essential. However, traditional manual and machine vision method detection have the problems of low detection accuracy and slow efficiency. Based on the problems of the above detection methods, this paper proposes the detection method based on Faster R-CNN fusion model to improve the detection performance. First, the designed image preprocessing methods, U-Net and ResNet50, are combined and used as the feature extraction part of the whole network with the aim of obtaining more distinct feature information. Secondly, the region proposals the network part to generate initial anchors of specific size by K-means clustering algorithm instead of manual setting, which can have an improvement on the accuracy and rate of classification and position prediction of bounding boxes. The experimental show that on our dataset, improved Faster R-CNN achieves 83% mAp, which proving the applicability of the model proposed in this paper.
Dynamic search control-based particle swarm optimization for project scheduling problems

Many machinery manufacturings are categorized as multi-mode resource-constrained project scheduling problems which have attracted significant interest in recent years. It has been shown that such problems are non-deterministic polynomial-time-hard. Particle swarm optimization is one of the most commonly used metaheuristic. Multi-mode resource-constrained project scheduling problems comprise two sub-problems, namely, an activity operating priority and an activity operating mode sub-problems; hence, two particle swarm optimizations are used to solve these two sub-problems. In solving the activity priority sub-problem, a designed global guidance ratio is involved to control the particle’s search behavior. Restated, guiding a diversification search at the beginning stage and conducting an intensification search at latter stage are controlled by adjusting the global guidance ratio. The particle swarm optimization combined with the global guidance ratio mechanism is named global guidance ratio–particle swarm optimization herein. Meanwhile, a non-fixed global guidance ratio adjustment is also suggested to further enhance the search performance. Moreover, different communication topologies for balancing the convergence of using global and local topologies are also suggested in global guidance ratio–particle swarm optimization to further improve the search efficiency. The performance of the proposed global guidance ratio–particle swarm optimization scheme is evaluated by solving all the multi-mode resource-constrained project scheduling problem instances in Project Scheduling Problem Library. It is shown that the scheduling solutions are in good agreement with those presented in the literatures. Hence, the effectiveness of the proposed global guidance ratio–particle swarm optimization scheme is confirmed.
Scalp Electroacupuncture Promotes Angiogenesis after Stroke in Rats by Activation of Wnt/β-Catenin Signal Pathway

Background Scalp acupuncture is a contemporary acupuncture method based on the fundamental theories of traditional acupuncture, which has been widely used in patients with stroke in China. However, the effectiveness is controversial due to lack of solid experimental evidence. Methods In this study, a rat model of cerebral ischemia-reperfusion injury (CIRI) was established by the middle cerebral artery occlusion/recirculation. The efficacy of scalp acupuncture against CIRI was evaluated by the mNSS scores, TTC staining for brain slices, and laser Doppler perfusion imaging. Immunohistochemical staining for angiogenetic factors indicated the vascularization after CIRI, including VEGF, Ang2, and bFGF. Activation of the Wnt/β-catenin signaling pathway and p-GSK3β (ser9)/VEGF pathway in the injured brain tissues was assessed by western blotting and qRT-PCR. Results On the 7, 14, and 21 days after CIRI, scalp acupuncture could reduce the mNSS scores, decrease the cerebral infarction area, and accelerate the recirculation of ischemic brain tissues. VEGF, FLK1, bFGF, and Ang2 were upregulated on both the mRNA and protein levels in the ischemic brain tissues of the AC group, suggesting that the recirculation might result from angiogenesis, which was also confirmed with the IHC staining in the angiogenetic markers of VEGF, Ang2, and bFGF. Moreover, Wnt3a, β-catenin, and cyclin D1 were also upregulated on both the mRNA and protein levels in the ischemic brain tissues of the AC group on day 7, 14, and 21, indicating that the Wnt/β-catenin signaling pathway was activated after the treatment of scalp acupuncture. In contrast, dikkoppf-1 (DKK1) pretreatment, a specific inhibitor for the Wnt/β-catenin signaling pathway, inactivated the Wnt3a/β-catenin signaling pathway and exacerbated the infarct size induced by the cerebral IR injury on day 7. Conclusion Together, our findings demonstrated a mechanism whereby scalp acupuncture led to the activation of Wnt/β-catenin signaling pathway, promoting angiogenetic factor expression and restoring blood perfusion in the ischemic zone.
Overview of (f)MRI Studies of Cognitive Aging for Non-Experts: Looking through the Lens of Neuroimaging

This special issue concerning Brain Functional and Structural Connectivity and Cognition aims to expand our understanding of brain connectivity. Herein, I review related topics including the principle and concepts of functional MRI, brain activation, and functional/structural connectivity in aging for uninitiated readers. Visuospatial attention, one of the well-studied functions in aging, is discussed from the perspective of neuroimaging.