Miracle-dz/UniFAR-FTUs huggingface dataset:
Exploratory study on the safety and effectiveness of Yizhi Qingxin Decoction (capsules) in the treatment of hypertension in the elderly with mild cognitive impairment (deficiency of kidney essence syndrome)

Abstract Background: Hypertension in the elderly with cognitive impairment has been one of the global health issues. Mild cognitive impairment (MCI) is the state of transition between the normal aging process and cognitive changes of unformed dementia. Diagnosis and treatment of MCI are the keys to prevent dementia, and hypertension is one of the important influencing factors of MCI. Our preclinical experiment found that Yizhi Qingxin Decoction (YQD) could effectively reduce the blood pressure of spontaneously hypertensive rats (SHR), improve their spatial learning and memory abilities in Morris water maze, and play a neuroprotective role. The objective is to estimate the safety and efficacy of YQD (capsules) in the treatment of hypertension in the elderly with MCI (deficiency of kidney essence syndrome) through this study. Methods: According to the random number generated by the block random method, 100 participants will be randomly and equally divided into the treatment group (YQD) or the control group (Ginkgo biloba extract tablets). The conversion rate of dementia will be used as the main evaluating indicator by the CDR scale. The MoCA scale, MMSE scale, ADCS-MCI-ADL-24 scale, CGIC-KDS scale, and 24-h ambulatory blood pressure will be used as the secondary evaluating indicator. Safety will be evaluated based on specific manifestations of adverse reactions and the incidence of adverse events. Objective: The objective is to estimate the curative effect of YQD (capsules) on hypertension in the elderly with MCI (deficiency of kidney essence syndrome), and to evaluate the safety of its clinical application. Trial registration: Chinese Clinical Trial Registry (ICTRP member): ChiCTR2000030292.
Flash Gas Chromatography in Tandem with Chemometrics: A Rapid Screening Tool for Quality Grades of Virgin Olive Oils

This research aims to develop a classification model based on untargeted elaboration of volatile fraction fingerprints of virgin olive oils (n = 331) analyzed by flash gas chromatography to predict the commercial category of samples (extra virgin olive oil, EVOO; virgin olive oil, VOO; lampante olive oil, LOO). The raw data related to volatile profiles were considered as independent variables, while the quality grades provided by sensory assessment were defined as a reference parameter. This data matrix was elaborated using the linear technique partial least squares-discriminant analysis (PLS-DA), applying, in sequence, two sequential classification models with two categories (EVOO vs. no-EVOO followed by VOO vs. LOO and LOO vs. no-LOO followed by VOO vs. EVOO). The results from this large set of samples provide satisfactory percentages of correctly classified samples, ranging from 72% to 85%, in external validation. This confirms the reliability of this approach in rapid screening of quality grades and that it represents a valid solution for supporting sensory panels, increasing the efficiency of the controls, and also applicable to the industrial sector.
Electrochemotherapy of cholangiocellular carcinoma at hepatic hilum: a case report.

OBJECTIVE Cholangiocarcinoma (CCA) is the second most common primary hepatic malignancy after hepatocellular carcinoma. The current standard palliative treatment, chemotherapy regimen with gemcitabine and cisplatin, prolongs overall survival only of a few months. Established locoregional therapies are not a curative option or an alternative to surgery in the treatment of CCA. We report a case of a patient affected by a cholangiocellular carcinoma at hepatic hilum treated by Electrochemotherapy (ECT) at our oncologic center. CASE PRESENTATION A 71 years old male affected by a CCA at hepatic hilum was treated with ECT according to ESOPE guidelines. No complications occurred during ECT procedure. The patient was discharged after 10 days. The functional MR evaluation at 2 and at 4 months post-treatment showed a significant response without significant post-treatment adverse events. The Computed tomography (CT) assessment after 18 months did not show progression of disease. CONCLUSIONS ECT is safe and its use could be suggested as a palliative treatment of advanced neoplastic lesions in which radical surgical treatment is not possible.
Light Attenuation by Limestone Rock and Its Constraint on the Depth Distribution of Endolithic Algae and Cyanobacteria

To understand how light attenuation in limestone rock constrains the distribution and abundance of endolithic photoautotrophic organisms, we examined light‐level profiles in 25 rock samples containing endolithic algae and cyanobacteria. We collected samples from three representative cliff sites along the Niagara Escarpment, Canada, using methods that allowed us to make comparisons among as well as within sites. Photosynthetically active radiation profiles were generated by manufacturing cavities in the bottom of thick slabs of surface rock and enclosing radiation sensors in the cavities. The thickness of the flat layer of limestone between the sensor and a light source was then abraded in steps of ca. 0.5 mm. An exponential curve was fitted to the measurements for each sample to allow statistical comparisons of the light levels at different depths. We examined the correlations between physical characteristics of the rock and light attenuation and between light attenuation and the maximum depth, relative biomass, and taxonomic richness of endolithic algae and cyanobacteria. The depth at which 0.01% of available light remained varied from 2.1 to 4.5 mm at different sites, but there was large within‐site variability on intermediate and small spatial scales. In contrast, the maximum depth of photoautotrophic endoliths was homogeneous within sites but different among sites (1.1–3.5 mm). Although the endoliths all have access to light, there was no significant correlation between the limit of their depth penetration and threshold quantum flux densities, indicating that the mechanisms controlling distribution and abundance of endolithic photoautotrophs are as complicated as those found for aquatic algae and cyanobacteria.
Dietary interventions in blood pressure lowering: current evidence in 2020.

Nutrition modification is one of the cornerstones of arterial hypertension (AH) treatment. Current American and European guidelines recommend to ingest fruits, vegetables, whole grains, and low-fat dairy products, and decrease the consumption of red meat, sugar, and trans fats. The aim of our review is to summarize the available evidence on the topic of dietary patterns associated with lower blood pressure (BP). Dietary Approach to Stop Hypertension (DASH) diet is able to lower BP equally or even more significantly than some antihypertensive drugs. The Mediterranean diet also leads to the significant reduction in BP. Vegans and vegetarians are showed to have a lower prevalence of AH than omnivores. Caloric restriction may decrease BP in normotensive, prehypertensive and hypertensive populations. BP can also be lowered by certain nutraceuticals (beetroot juice, magnesium, vitamin C, catechin-rich beverages, soy isoflavones etc). Conclusions. Diet effects on BP are mediated by the decrease of body weight, amelioration of inflammation, increase of insulin sensitivity, and antihypertensive effects of some single nutrients. Vegetarian and vegan diets have robust evidence proving their ability to reduce BP. The existence of floor effect makes these diets usable for normo- and prehypertensive people with high risk of developing AH. The dietary and nutraceutical approach to the BP lowering never has to substitute the drug treatment when the latter is needed.
Muscle-derived Myoglianin regulates Drosophila imaginal disc growth

Organ growth and size are finely tuned by intrinsic and extrinsic signaling molecules. In Drosophila, the BMP family member Dpp is produced in a limited set of imaginal disc cells and functions as a classic morphogen to regulate pattern and growth by diffusing throughout imaginal discs. However, the role of TGFβ/Activin-like ligands in disc growth control remains ill-defined. Here, we demonstrate that Myoglianin (Myo), an Activin family member, and a close homolog of mammalian Myostatin (Mstn), is a muscle-derived extrinsic factor that uses canonical dSmad2-mediated signaling to regulate wing size. We propose that Myo is a myokine that helps mediate an allometric relationship between muscles and their associated appendages.
Autophagy-Related 2 Regulates Chlorophyll Degradation under Abiotic Stress Conditions in Arabidopsis

Chloroplasts are extraordinary organelles for photosynthesis and nutrient storage in plants. During leaf senescence or under stress conditions, damaged chloroplasts are degraded and provide nutrients for developing organs. Autophagy is a high-throughput degradation pathway for intracellular material turnover in eukaryotes. Along with chloroplast degradation, chlorophyll, an important component of the photosynthetic machine, is also degraded. However, the chlorophyll degradation pathways under high light intensity and high temperature stress are not well known. Here, we identified and characterized a novel Arabidopsis mutant, sl2 (seedling lethal 2), showing defective chloroplast development and accelerated chlorophyll degradation. Map-based cloning combined with high-throughput sequencing analysis revealed that a 118.6 kb deletion region was associated with the phenotype of the mutant. Complementary experiments confirmed that the loss of function of ATG2 was responsible for accelerating chlorophyll degradation in sl2 mutants. Furthermore, we analyzed chlorophyll degradation under abiotic stress conditions and found that both chloroplast vesiculation and autophagy take part in chlorophyll degradation under high light intensity and high temperature stress. These results enhanced our understanding of chlorophyll degradation under high light intensity and high temperature stress.
Anthropogenic Correlates of Species Richness in Southeastern Ontario Wetlands

We examined the relationship between the richness of four different wetland taxa (birds, mammals, herptiles, and plants) in 30 southeastern Ontario, Canada wetlands and two anthropogenic factors: road construction and forest removal/conversion on adjacent lands. Data were obtained from two sources: road densities and forest cover from 1:50,000 Government of Canada topographic maps and species lists and wetland areas from Ontario Ministry of Natural Resources wetland evaluation reports. Multiple regression analysis was used to model the relationships between species richness and wetland area, road density, and forest cover. Our results show a strong positive relationship between wetland area and species richness for all taxa. The species richness of all taxa except mammals was negatively correlated with the density of paved roads on lands up to 2 km from the wetland. Furthermore, both herptile and mammal species richness showed a strong positive correlation with the proportion of forest cover on lands within 2 km. These results provide evidence that at the landscape level, road construction and forest removal on adjacent lands pose significant risks to wetland biodiversity. Furthermore, they suggest that most existing wetland policies, which focus almost exclusively on activities within the wetland itself and/or a narrow buffer zone around the wetland perimeter, are unlikely to provide adequate protection for wetland biodiversity.
Stereotactic radiotherapy for hepatocellular carcinoma: Expanding the multidisciplinary armamentarium

Hepatocellular carcinoma (HCC) is the fifth most common malignancy worldwide and the third most common cause of cancer‐related death. Long‐term prognosis remains poor with treatment options frequently limited by advanced tumor stage, tumor location, or underlying liver dysfunction. Stereotactic ablative body radiotherapy (SABR) utilizes technological advances to deliver highly precise, tumoricidal doses of radiation. There is an emerging body of literature on SABR in HCC demonstrating high rates of local control in the order of 80–90% at 3 years. SABR is associated with a low risk of radiation‐induced liver disease or decompensation in appropriately selected HCC patients with compensated liver function and is now being incorporated into guidelines as an additional treatment option. This review outlines the emerging role of SABR in the multidisciplinary management of HCC and summarizes the current evidence for its use as an alternative ablative option for early‐stage disease, as a bridge to transplant, and as palliation for advanced‐stage disease. We outline specific considerations regarding patient selection, toxicities, and response assessment. Finally, we compare current international guidelines and recommendations for the use of SABR and summarize ongoing studies.
Use of life tables and LC50 tests to evaluate chronic and acute toxicity effects of copper on the marine copepod Tisbe furcata (Baird)

Cohorts of the epiphytic marine copepod Tisbe furcata were chronically exposed to copper in life-table experiments to test whether ecologically relevant impacts can occur at sublethal concentrations. Data on fecundity, longevity, and rate of development were used to calculate rm – the intrinsic rate of natural increase. Acute toxicity tests (96-h LC50 tests) were done to compare the concentrations of copper affecting individual lethality and population biology. The LC50 value for Tisbe furcata nauplii was 2.8 μM copper (178 μg/L). The results from the life-table experiments show that 0.9 μM copper (32% of LC50) can cause significant negative effects on demographic parameters (total production of nauplii, life span, and reproductive period for fertile females) and reduce the percentage of fertile females leading to a 61% reduction of rm. However, rm was still positive at 0.9 μM copper, and the net reproductive rate (Ro) indicated a fivefold increase in population size from one generation to the next. Although there were no significant effects of copper at 0.5 μM (18% of LC50), there was a negative trend in almost all the demographic parameters, indicating that the observed 10% reduction of rm at this concentration was an effect of copper. For the substances tested so far with both acute LC50 tests and life-table experiments, rm was not reduced at concentrations below LC50/10. When life-table experiments are used as part of environmental hazard assessments, concentrations below LC50/10 should be tested to detect substances that are potentially harmful to the environment at sublethal concentrations, rather than testing concentrations close to LC50.
Ultrasound Treatment of Buckwheat Grains Impacts Important Functional Properties of Resulting Flour

The benefit of not containing the gluten complex protein also provides problems with the achievement of typical and proper texture, especially in bakery products. Ultrasound (US) treatment has been previously studied on buckwheat as assistance treatment facilitating the release of antioxidant compounds. However, there is no study regarding the changes occurring in US-treated buckwheat grains regarding the structure-creating capacity, like water absorption, gelling, and pasting. The aim of this study is to the impact of US-treatment of buckwheat grains at 1:10, 1:5, and 1:2.5 solid:liquid ratio (in water). The particle size distribution, water absorption index (WAI), water solubility index (WSI), swelling power (SP), pasting characteristics, color, soluble, insoluble and total polyphenols content (SPC, IPC, TPC) and antioxidant activity (DPPH) were assessed in resulting flours. US-treatment caused specific agglomeration, resulting in bigger particles for 1:5, and 1:2.5 ratio treated samples, while higher dilution (1:10) increased smaller particle size fractions. The WAI and SP were the highest for the1:5 solid:liquid ratio sample, and the same sample revealed the highest peak viscosity, breakdown, and setback values. The ultrasound treatment increased the WSI, which was positively correlated with insoluble polyphenols content. The soluble polyphenols content decreased, and insoluble polyphenols content increased in all ultrasound treated samples. The DPPH scavenging activity remaining in grain after US treatment was lowered compared to the control sample. The relocation of pigments resulted in a redness and yellowish increase in all treated samples, while lightness was also increased but was most pronounced for a 1:10 ratio treated sample. The results suggest that ultrasound treatment of grain can improve the essential functional properties of buckwheat flour.
SARS, MERS and COVID-19: clinical manifestations and organ-system complications: a mini review

ABSTRACT Middle East Respiratory Syndrome (MERS), Severe Acute Respiratory Syndrome (SARS) and Coronavirus Disease 2019 (COVID-19) are caused by three distinct coronaviruses belonging to the same genus. COVID-19 and its two predecessors share many important features in their clinical presentations, and in their propensity for progression to severe disease which is marked by high rates of morbidity and mortality. However, comparison of the three viral illnesses also reveals a number of specific differences in clinical manifestations and complications, which suggest variability in the disease process. This narrative review delineates the pulmonary, cardiac, renal, gastrointestinal, hepatic, neurological and hematologic complications associated with these three respiratory coronaviruses. It further describes the mechanisms of immune hyperactivation—particularly cytokine release syndrome—implicated in the multi-organ system injury seen in severe cases of MERS, SARS and COVID-19.
Instrument to Identify Food Neophobia in Brazilian Children by Their Caregivers

This study aimed to develop a specific instrument to evaluate food neophobia focused on Brazilian children and to perform the content validation and internal semantic consistency and reproducibility evaluation of the instrument. Three steps were necessary to conduct the study: (i) development of the instrument, (ii) internal validation (content validation and semantic evaluation) of the food neophobia instrument using 22 experts in the first round and 20 of them in the second round, (iii) evaluation of the internal consistency and reproducibility of the instrument with the children’s caregivers, using the test–retest (where the same caregiver—n = 22—answered twice, with 24 h interval) and comparing responses between two caregivers (n = 44) of the same children (n = 22). We developed an instrument in Brazilian–Portuguese to evaluate children’s food neophobia based on the caregivers’ perceptions with 25 items divided into three domains (neophobia in general, neophobia for fruits and neophobia for vegetables). Our results indicated that the instrument has excellent internal consistency (>0.9) and reproducibility (>0.9) when answered by the caregiver who knows the child’s eating habits, indicating reliability to be applied in Brazil. In addition, when the two caregivers answered the instrument, we found a good reproducibility (>0.6), confirming the possibility to be answered by one of the caregivers. Further studies are necessary to complete external validation with a representative sample of the target group in Brazil, showing nationwide the profile of the population. The potential of a neophobia study would contribute to the implementation of effective strategies and guidelines to support parents and health professionals, especially those involved in health and nutrition, to identify traces of food neophobia or neophobic behavior. By accurately measuring food neophobia in children, families can prevent nutritional deficiencies throughout adolescence and adulthood, improving eating habits. Children usually have neophobias similar to the ones presented by their parents—and when early detected, these neophobias can be addressed.
Comparison of Rumen Microbiota and Serum Biochemical Indices in White Cashmere Goats Fed Ensiled or Sun-Dried Mulberry Leaves

Mulberry leaves, which have high nutritional value, have not been fully utilized. Few research systems have indicated whether mulberry leaves can replace traditional feed ingredients in goats. In this study, we investigated the effects of feeding white cashmere goats ensiled (Group E) or sun-dried mulberry leaves (Group S) on changes in ruminal microbial communities, rumen fermentation parameters and serum biochemical indices. The control group (Group C) received a typical total mixed ration (TMR). 16S rRNA gene sequencing revealed 209 genera belonging to 19 bacterial phyla dominated by Firmicutes and Bacteroidetes. Only the relative abundances of Erysipelotrichaceae_UCG-009 were significantly different among the three groups (p < 0.05). Physiological and biochemical findings revealed that only the serum leptin concentrations were significantly decreased when mulberry leaves were added to the diets (p < 0.05). Correlation analysis revealed that Ruminococcus_2 were significantly positively correlated with the butyrate concentration. These findings suggested that supplementation with mulberry leaves only induced minor changes in the abovementioned indicators, implying that the rumen fermentation status was still stable after adding mulberry leaves to the diets.
Termite Societies Promote the Taxonomic and Functional Diversity of Archaeal Communities in Mound Soils

Recent studies involving microbial communities in termite mounds have been more focused on bacteria and fungi with little attention given to archaea, which play significant roles in nutrient cycling. Thus, we aimed at characterizing the archaeal taxonomic and functional diversity in two termite mound soils using the shotgun sequencing method with the assumption that termite activities could promote archaeal diversity. Our findings showed that termite mound soils have archaeal groups that are taxonomically different from their surrounding soils, with Euryarchaeota, Korarchaeota, and Nanoarchaeota being predominant while Thaumarchaeota and Crenarchaeota were predominant in the surrounding soils. Additionally, the observed nutrient pathways: phosphorus, nitrogen, and sulfur were all significantly more predominant in termite mound soils than in their comparative surrounding soils. Alpha diversity showed that archaea were not significantly different within termite mound soils and the surrounding soils. The beta diversity revealed significant differences in the archaeal taxonomic composition and their functional categories between the termite mounds and surrounding soils. Our canonical correspondence analysis revealed that the distribution of archaeal communities was likely dependent on the soil properties. Our results suggested that termite activities may promote the diversity of archaea; with some of our sequences grouped as unclassified archaea, there is a need for further research to unveil their identity.
Use of saliva sample to detect C‐reactive protein in children with pneumonia

Serum C‐reactive protein (CRP) is a sensitive biomarker for inflammation and is broadly used to clinically diagnose infectious diseases, including pneumonia. However, blood sampling is fraught with technical difficulties in children. The salivary analysis may be a potential diagnostic tool, as it is noninvasive, patient‐friendly, and easy to perform in children. This study aimed to evaluate the use of salivary CRP as a biomarker for children with pneumonia.