Miracle-dz/UniFAR-FTUs huggingface dataset:
Multilocus sequence analysis revealed a high genotypic diversity of Aeromonas hydrophila infecting fish in Uganda.

A multilocus sequence analysis (MLSA) was carried out to delineate Aeromonas hydrophila from fish in Uganda. Five housekeeping genes including recA, gyrB, metG, gltA and pps; and the 16S rRNA gene were amplified and sequenced from a total of nine A. hydrophila isolates. The obtained sequences were edited, and consensus sequences generated for each gene locus. The housekeeping gene sequences were concatenated and phylogenetic analysis performed in MEGA version 7.0. 2. Pairwise distances ranged from 0.000 to 0.118, highest within the gltA gene locus and lowest within the 16S rRNA gene. The average evolutionary diversity within isolates from the same source ranged between 0.002 and 0.037, and it was 0.033 between the different sources. Similar tree topologies were obtained from the different gene loci with recA, metG and gyrB being more consistent in discriminating isolates according to sources while the 16S rRNA gene had the lowest resolution. The concatenated tree had the highest discriminatory power. This study revealed that A. hydrophila strains infecting fish in Uganda are of diverse genotypes suggesting different sources of infection in a given outbreak. Efforts to minimize spread of the bacteria across sources should be emphasized to control infections of mixed genotypes.
The Role of the Basement Plate in Physeal Bar Formation

Background: Physeal fractures and resultant physeal bars can pose significant problems in skeletal development for the injured growing child. Although now well-recognized, only a small body of experimental literature covering this problem is available. The goal of this study was to help further develop an understanding of the different regions of the physis and the way in which each region responds to injury/fracture. Methods: This Institutional Animal Care and Use Committee (IACUC)-approved study assessed bar formation using radiologic and histologic methods and measured leg lengths of skeletally immature rats. The right tibia was used as the control to measure leg length discrepancy (LLD), and the left tibia received either a fracture only (F), an epiphyseal scrape (ES), an epiphyseal drilling procedure (ED), or metaphyseal drilling (MD). Radiographs and LLD measurements were obtained at postoperative days 0, 21, and 56. Results: A significant LLD was present at day 56 in the ED group (P=0.01). Radiographic identification of bars showed significant evidence of bar formation for the ES and ED groups at 21 days and the ED group at 56 days (P<0.05). Histologic examination showed a high incidence of histologic physeal bar formation in the ES, ED, and MD groups at 21 and 56 days. Conclusions: Findings showed that the physis was able to continue to grow following an injury to the physis’ hypertrophic region. MD produced little effects with few physeal bars and little LLD. By postoperative day 56, ED animals showed greater LLD than ES animals. Penetration of the basement plate was more likely to lead to bar formation/growth retardation than was ablation of the epiphyseal region of the physis (including resting cells). Clinical Relevance: Data presented here provides insight into the importance of different regions of the physis and its repair/continued growth after physeal fracture. We suggest that a better understanding of the physiological cause of physeal arrest after physeal fracture will be important for the development of treatments to prevent physeal arrest or to treat physeal arrest after it occurs.
A Class of Weighted TSPs with Applications

Motivated by applications to poaching and burglary prevention, we define a class of weighted Traveling Salesman Problems on metric spaces. The goal is to output an infinite (though typically periodic) tour that visits the n points repeatedly, such that no point goes unvisited for "too long. " More specifically, we consider two objective functions for each point x. The maximum objective is simply the maximum duration of any absence from x, while the quadratic objective is the normalized sum of squares of absence lengths from x. For periodic tours, the quadratic objective captures the expected duration of absence from x at a uniformly random point in time during the tour. The overall objective is then the weighted maximum of the individual points' objectives. When a point has weight w_x, the absences under an optimal tour should be roughly a 1/w_x fraction of the absences from points of weight 1. Thus, the objective naturally encourages visiting high-weight points more frequently, and at roughly evenly spaced intervals. We give a polynomial-time combinatorial algorithm whose output is simultaneously an O(log n) approximation under both objectives. We prove that up to constant factors, approximation guarantees for the quadratic objective directly imply the same guarantees for a natural security patrol game defined in recent work.
Application of Lexicographic Optimization Method to Integrated Vehicle Control Systems

In this paper, a general control allocation (CA) algorithm is proposed based on a lexicographic optimization (LO) strategy for a vehicle's longitudinal and lateral control. The primary objective of this CA is to distribute the torque adjustments of the lateral controller such that the error between the desired and actual forces and moments at the vehicle's center of gravity is minimized, while maintaining the longitudinal tire slip ratios close to a desired range. Presence of various constraints in practical systems can significantly increase the complexity and effort of properly adjusting the tuning parameters in the objective function. Hence, an LO method is used to prioritize the objectives. Lateral stability of the vehicle has the highest priority and is subject to the constraint of maintaining small tire slip ratios. The second priority of CA is assigned to minimize the adjustment torques. The proposed LO-based approach reduces exhaustive vehicle testing commonly required for tuning of the separately designed controllers and the cost and time of the implementation. In addition, it makes the algorithm easily transferable from one vehicle to another by reducing the number of tuning parameters. Simulation and experimental results are presented to show the effectiveness of the proposed approach.
Automatically Determining the Confocal Parameters From OCT B-Scans for Quantification of the Attenuation Coefficients

The attenuation coefficient is a relevant biomarker for many diagnostic medical applications. Recently, the Depth-Resolved Confocal (DRC) technique was developed to automatically estimate the attenuation coefficients from Optical Coherence Tomography (OCT) data with pixel-level resolution. However, DRC requires that the confocal function parameters (i.e., focal plane location and apparent Rayleigh range) be known a priori. In this paper, we present the autoConfocal algorithm: a simple, automatic method for estimating those parameters directly from OCT imagery when the focal plane is within the sample. We present autoConfocal+DRC results on phantom data, ex-vivo biological tissue data, and in-vivo clinical data.
The effect of haemodynamic and peripheral vascular variability on cardiac output monitoring: thermodilution and non‐invasive pulse contour cardiac output during cardiothoracic surgery

While haemodynamic variability interferes with the assumption of constant flow underlying thermodilution cardiac output calculation, variability in (peripheral) arterial vascular physiology may affect pulse contour cardiac output methods. We compared non‐invasive finger arterial pressure‐derived continuous cardiac output measurements (Nexfin®) with cardiac output measured using thermodilution during cardiothoracic surgery and determined the impact of cardiovascular variability on either method. We compared cardiac output derived from non‐invasive finger arterial pressure with cardiac output measured by thermodilution at four grades (A–D) of cardiovascular variability. We defined Grade A data as heart rate and mean arterial pressure variability < 5% and the absence of arrhythmias (implying stable flow) and Physiocal® interval (as measure of variability in finger arterial physiology) > 30 beats. Grade B included all levels of heart rate/mean arterial pressure variability and arrhythmias (Physiocal < 30 excluded). Grade C included all Physiocal intervals (heart rate/mean arterial pressure variability > 5% and arrhythmias excluded). Grade D included all data. Comparison results were quantified as percentage errors. We analysed measurements in 27 patients undergoing coronary artery bypass surgery. Before extracorporeal circulation, the percentage error was 23% (n = 14 patients) in grade A, 28% (n = 20) in grade B, 32% (n = 22) in grade C and 37% (n = 26) in grade D, with a significant increase in variance (p = 0.035). Bias did not differ between grades. After extracorporeal circulation (n = 27), percentage errors became larger, but were not different between grades. Variability during cardiothoracic surgery affected the comparison between thermodilution and non‐invasive finger arterial pressure‐derived cardiac output. When the main sources of variability were included, percentage errors were large. Future cardiac output methodology comparison studies should report haemodynamic variability.
Key Brain Network Nodes Show Differential Cognitive Relevance and Developmental Trajectories during Childhood and Adolescence

Abstract Human adolescence is a period of rapid changes in cognition and goal-directed behavior, and it constitutes a major transitional phase towards adulthood. One of the mechanisms suggested to underlie the protracted maturation of functional brain networks, is the increased network integration and segregation enhancing neural efficiency. Importantly, the increasing coordinated network interplay throughout development is mediated through functional hubs, which are highly connected brain areas suggested to be pivotal nodes for the regulation of neural activity. To elucidate brain hub development during childhood and adolescence, we estimated voxel-wise eigenvector centrality (EC) using functional magnetic resonance imaging (fMRI) data from two different psychological contexts (resting state and a working memory task), in a large cross-sectional sample (n = 754) spanning the age from 8 to 22 years, and decomposed the maps using independent component analysis (ICA). Our results reveal significant age-related centrality differences in cingulo-opercular, visual, and sensorimotor network nodes during both rest and task performance, suggesting that common neurodevelopmental processes manifest across different mental states. Supporting the functional significance of these developmental patterns, the centrality of the cingulo-opercular node was positively associated with task performance. These findings provide evidence for protracted maturation of hub properties in specific nodes of the brain connectome during the course of childhood and adolescence and suggest that cingulo-opercular centrality is a key factor supporting neurocognitive development.
Advanced Control of Grid-Connected Current Source Converter Under Unbalanced Grid Voltage Conditions

There are technical challenges when the grid-connected current source converter operates under unbalanced grid voltage conditions. The negative sequence component of the unbalanced grid voltage would result in the low-frequency ripple at dc side, as well as current harmonics at ac side. It has an adverse impact on the system operation. In order to solve the problem, the system mathematical model is established in this paper. The limitation of the conventional control strategy is clearly clarified. And then, the improved control strategy is discussed from the viewpoint of constant instantaneous active power. In order to further enhance the system performance, a new control strategy is proposed. Instead of the complicated instantaneous power theory, the proposed solution is a direct control strategy. Both the low-frequency dc-side ripple and ac-side current harmonics can be significantly reduced with a simple control structure, due to the direct control concept. Finally, the comparison evaluation of the conventional and proposed control strategies is carried out. The experimental results verify the effectiveness of the proposed solution.
Glasdegib in combination with cytarabine and daunorubicin in patients with AML or high‐risk MDS: Phase 2 study results

Glasdegib is a Hedgehog pathway inhibitor. This ongoing, open‐label, phase 2 study (NCT01546038) evaluated glasdegib plus cytarabine/daunorubicin in patients with untreated acute myeloid leukemia (AML) or high‐risk myelodysplastic syndromes (MDS). Patients received glasdegib 100 mg orally, once daily in continuous 28‐day cycles from day −3, with intravenous cytarabine 100 mg/m2 on days 1‐7 and daunorubicin 60 mg/m2 on days 1‐3. Patients in remission then received consolidation therapy (2‐4 cycles of cytarabine 1 g/m2 twice daily on days 1, 3, 5 of each cycle), followed by maintenance glasdegib (maximum 6 cycles). Primary endpoint was complete remission (CR) in patients aged ≥55 years. Secondary endpoints included overall survival (OS), safety and outcome by mutational status. Patients had a median (range) age of 64.0 (27‐75) years, 60.0% were male, and 84.5% were white. In 69 evaluable patients, 46.4% (80% confidence interval [CI]: 38.7‐54.1) achieved investigator‐reported CR. Among patients ≥55 years old (n = 60), 40.0% (80% CI 31.9‐48.1) achieved CR. Among all 69 patients, median OS was 14.9 (80% CI 13.4‐19.3) months, with 12‐month survival probability 66.6% (80% CI 58.5‐73.4). The most common treatment‐related adverse events (≥50% patients) were diarrhea and nausea. There were no significant associations between mutational status (12 genes) and clinical response, suggesting potential benefit across diverse molecular profiles. Glasdegib plus cytarabine/daunorubicin was well tolerated and associated with clinical activity in patients with untreated AML or high‐risk MDS. A randomized phase 3 trial of glasdegib in combination with chemotherapy (7 + 3 schedule) is ongoing.
Hydroxysafflor Yellow A Suppresses Platelet Activating Factor-Induced Activation of Human Small Airway Epithelial Cells

Hydroxysafflor yellow A (HSYA) is a chemical component isolated from the Chinese medicine Carthamus tinctorius L. HSYA has numerous pharmacological effects, including protecting against and mitigating some respiratory diseases such as acute lung injury and chronic obstructive pulmonary disease; however, its effect on asthma remains unclear. We previously found that HSYA attenuated ovalbumin-induced allergic asthma in guinea pigs. Platelet activating factor (PAF) is a phospholipid mediator of inflammation and an important factor in the pathological process of asthma. In this study, we investigated the anti-inflammatory effects of HSYA and its underlying mechanisms in PAF-induced human small airway epithelial cells (HSAECs). PAF-activated cells were pretreated with HSYA and/or the PAF receptor inhibitor, ginkgolide B, and we observed changes in the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha, monolayer permeability of HSAECs, and inflammatory signaling pathways. HSYA attenuated the PAF-induced increase in expression of inflammatory factors and destruction of cell-barrier function, and inhibited the expression of protein kinase C, mitogen-activated protein kinases, activator protein-1, and nuclear factor-κB activation induced by PAF. These findings suggest that HSYA may represent a potential new drug for the treatment of asthma.
Implementation of Low Cost Stereo Humanoid Adaptive Vision for 3D Positioning and Distance Measurement for Robotics Application with Self-Calibration

Robots are getting smarter everyday with the implementation of computer vision system in it. It is now highly required for any robot to have a natural vision system or more likely humanoid vision system to interact with real life incidents. On the perspective of such imaging and vision, we propose an efficient method in order to determine the absolute view point of any desired image location. We used self calibration system and humanoid vision mechanism via stereo cameras to find the region of convergent of an object which with the help of a mathematical model can measure the distance of the object. With comparing different objects position it is also possible to determine the relative distance of the objects. Our system shows that, the real human eye tracking system used, can be possible for getting a realistic view of the image at the 3D point positioning.
A Compact Highly Efficient Hybrid Antenna Array for W-Band Applications

A W-band hybrid array with a compact size and a high efficiency is presented. With the aid of the joint design of the series-shunt feeding network and substrate integrated waveguide (SIW) blocked slots array, the array with 16 × 56 slots can be divided into eight areas, and then the bandwidth of impedance, gain, and radiation pattern can be improved effectively. Moreover, it has both the advantages of low profile over traditional SIW planar array and low loss over traditional air-filled rectangular waveguide (ARW) slot array. Due to the utilization of back-feeding technology of the proposed hybrid array instead of planar SIW divider, it could save a lot of area for radiating energy and lowering the insertion loss, thus resulting in the gain values higher than 30.7 dBi during the entire operational frequency coverage. It is corresponding to the total efficiency of 26.5%, which is higher than the traditional W-band SIW array due to the usage of the back feeding to conserve the radiation acreage and reduce the insertion loss. By designing the SIW slots array and series-shunt feeding network simultaneously, low sidelobe level characteristics of −16 and −20 dB in E- and H-planes have been successfully accomplished, respectively.
PRDM Histone Methyltransferase mRNA Levels Increase in Response to Curative Hormone Treatment for Cryptorchidism-Dependent Male Infertility

There is a correlation between cryptorchidism and an increased risk of testicular cancer and infertility. During orchidopexy, testicular biopsies are performed to confirm the presence of type A dark (Ad) spermatogonia, which are a marker for low infertility risk (LIR). The Ad spermatogonia are absent in high infertility risk (HIR) patients, who are treated with a gonadotropin-releasing hormone agonist (GnRHa) to significantly lower the risk of infertility. Despite its prevalence, little is known about the molecular events involved in cryptorchidism. Previously, we compared the transcriptomes of LIR versus HIR patients treated with and without hormones. Here, we interpreted data regarding members of the positive regulatory domain-containing (PRDM) family; some of which encoded histone methyltransferases that are important for reproduction. We found there were lower levels of PRDM1, PRDM6, PRDM9, PRDM13, and PRDM14 mRNA in the testes of HIR patients compared with LIR patients, and that PRDM7, PRDM9, PRDM12, and PRDM16 were significantly induced after GnRHa treatment. Furthermore, we observed PRDM9 protein staining in the cytoplasm of germ cells in the testes from LIR and HIR patients, indicating that the mRNA and protein levels corresponded. This result indicated that the curative hormonal therapy for cryptorchidism involved conserved chromatin modification enzymes.
Memristor-Based Synaptic Sampling Machines

Synaptic Sampling Machine (SSM) is a type of neural network model that considers biological unreliability of the synapses. We propose the circuit design of the SSM neural network which is realized through the memristive-CMOS crossbar structure with the synaptic sampling cell (SSC) being used as a basic stochastic unit. The increase in the edge computing devices in the Internet of things era, drives the need for hardware acceleration for data processing and computing. The computational considerations of the processing speed and possibility for the real-time realization pushes the synaptic sampling algorithm that demonstrated promising results on software for hardware implementation.
Casz1 controls higher-order nuclear organization in rod photoreceptors

Significance Eukaryotic cells depend on precise genome organization within the nucleus to maintain an appropriate gene-expression profile. Critical to this process is the packaging of functional domains of open and closed chromatin to specific regions of the nucleus, but how this is regulated remains unclear. In this study, we show that the zinc finger protein Casz1 regulates higher-order nuclear organization of rod photoreceptors in the mouse retina by repressing nuclear lamina function, which leads to central localization of heterochromatin. Loss of Casz1 in rods leads to an abnormal transcriptional profile followed by degeneration. These results identify Casz1 as a regulator of higher-order genome organization. Genome organization plays a fundamental role in the gene-expression programs of numerous cell types, but determinants of higher-order genome organization are poorly understood. In the developing mouse retina, rod photoreceptors represent a good model to study this question. They undergo a process called “chromatin inversion” during differentiation, in which, as opposed to classic nuclear organization, heterochromatin becomes localized to the center of the nucleus and euchromatin is restricted to the periphery. While previous studies showed that the lamin B receptor participates in this process, the molecular mechanisms regulating lamina function during differentiation remain elusive. Here, using conditional genetics, we show that the zinc finger transcription factor Casz1 is required to establish and maintain the inverted chromatin organization of rod photoreceptors and to safeguard their gene-expression profile and long-term survival. At the mechanistic level, we show that Casz1 interacts with the polycomb repressor complex in a splice variant-specific manner and that both are required to suppress the expression of the nuclear envelope intermediate filament lamin A/C in rods. Lamin A is in turn sufficient to regulate heterochromatin organization and nuclear position. Furthermore, we show that Casz1 is sufficient to expand and centralize the heterochromatin of fibroblasts, suggesting a general role for Casz1 in nuclear organization. Together, these data support a model in which Casz1 cooperates with polycomb to control rod genome organization, in part by silencing lamin A/C.
The permeation mechanism of organic cations through a CNG mimic channel

Several channels, ranging from TRP receptors to Gap junctions, allow the exchange of small organic solute across cell membrane. However, very little is known about the molecular mechanism of their permeation. Cyclic Nucleotide Gated (CNG) channels, despite their homology with K+ channels and in contrast with them, allow the passage of larger methylated and ethylated ammonium ions like dimethylammonium (DMA) and ethylammonium (EA). We combined electrophysiology and molecular dynamics simulations to examine how DMA interacts with the pore and permeates through it. Due to the presence of hydrophobic groups, DMA enters easily in the channel and, unlike the alkali cations, does not need to cross any barrier. We also show that while the crystal structure is consistent with the presence of a single DMA ion at full occupancy, the channel is able to conduct a sizable current of DMA ions only when two ions are present inside the channel. Moreover, the second DMA ion dramatically changes the free energy landscape, destabilizing the crystallographic binding site and lowering by almost 25 kJ/mol the binding affinity between DMA and the channel. Based on the results of the simulation the experimental electron density maps can be re-interpreted with the presence of a second ion at lower occupancy. In this mechanism the flexibility of the channel plays a key role, extending the classical multi-ion permeation paradigm in which conductance is enhanced by the plain interaction between the ions.