Miracle-dz/UniFAR-FTUs huggingface dataset:
A Novel Concept for Wearable, Modular and Soft Support Systems Used in Industrial Environments

This paper presents a novel concept for wearable support systems based on the approach of the Human Hybrid Robot (HHR), which can be adapted easily to the user and the activity. The concept focuses on modularity and makes intensive use of new manufacturing technologies like 3D-printing as well as flexible kinematics and textile components, in order to fit the system to different individuals and tasks as well as to increase human safety. The main idea can be applied to various applications. In this paper we are focusing on a functional exoskeleton prototype for the upper extremities. It comprises a Human-Machine-Interface (HMI) using a glove equipped with haptic sensors to measure grip force as well as force sensors in a coupling to the user at the forearm. This functional prototype was then successfully evaluated in a blind study with 20 test subjects.
Postpartum Smoking Cessation and Relapse Prevention Intervention

The aim of the study was to test the effectiveness of a postpartum smoking cessation and relapse prevention intervention. Structural equation modeling techniques were applied to evaluate the impact of the intervention on smoking behavior and on non-behavioral variables derived from the Transtheoretical Model (TTM). Women were randomized to an intervention (I) and control group (C). Smoking status, TTM-variables, and control variables were assessed four weeks, six and 12 months postpartum. Membership in the intervention group significantly predicted non-smoking and higher self-efficacy six months, but not one year postpartum, after controlling for demographic, smoking, and postpartum risk variables.
Natamycin Inhibits Vacuole Fusion at the Priming Phase via a Specific Interaction with Ergosterol

ABSTRACT The antifungal antibiotic natamycin belongs to the family of polyene antibiotics. Its antifungal activity arises via a specific interaction with ergosterol in the plasma membrane (te Welscher et al., J. Biol. Chem. 283:6393-6401, 2008). However, this activity does not involve disruption of the membrane barrier function, a well-known property of other members of the polyene antibiotic family, such as filipin and nystatin. Here we tested the effect of natamycin on vacuole membrane fusion, which is known to be ergosterol dependent. Natamycin blocked the fusion of isolated vacuoles without compromising the barrier function of the vacuolar membrane. Sublethal doses of natamycin perturbed the cellular vacuole morphology, causing the formation of many more small vacuolar structures in yeast cells. Using vacuoles isolated from yeast strains deficient in the ergosterol biosynthesis pathway, we showed that the inhibitory activity of natamycin was dependent on the presence of specific chemical features in the structure of ergosterol that allow the binding of natamycin. We found that natamycin inhibited the priming stage of vacuole fusion. Similar results were obtained with nystatin. These results suggest a novel mode of action of natamycin and perhaps all polyene antibiotics, which involves the impairment of membrane fusion via perturbation of ergosterol-dependent priming reactions that precede membrane fusion, and they may point to an effect of natamycin on ergosterol-dependent protein function in general.
New Fks Hot Spot for Acquired Echinocandin Resistance in Saccharomyces cerevisiae and Its Contribution to Intrinsic Resistance of Scedosporium Species

ABSTRACT Echinocandins represent a new antifungal group with potent activity against Candida species. These lipopeptides inhibit the synthesis of β-1,3-glucan, the major cell wall polysaccharide. Acquired resistance or reduced echinocandin susceptibility (RES) is rare and associated with mutations in two “hot spot” regions of Fks1 or Fks2, the probable β-1,3-glucan synthases. In contrast, many fungi demonstrate intrinsic RES for reasons that remain unclear. We are using Saccharomyces cerevisiae to understand the basis for RES by modeling echinocandin-Fks interaction. Previously characterized mutations confer cross-RES; we screened for mutations conferring differential RES, implying direct interaction of that Fks residue with a variable echinocandin side chain. One mutant (in an fks1Δ background) exhibited ≥16-fold micafungin and anidulafungin versus caspofungin RES. Sequencing identified a novel Fks2 mutation, W714L/Y715N. Equivalent W695L/Y696N and related W695L/F/C mutations in Fks1 generated by site-directed mutagenesis and the isolation of a W695L-equivalent mutation in Candida glabrata confirmed the role of the new “hot spot 3” in RES. Further mutagenesis expanded hot spot 3 to Fks1 residues 690 to 700, yielding phenotypes ranging from cross-RES to differential hypersusceptibility. Fks1 sequences from intrinsically RES Scedosporium species revealed W695F-equivalent substitutions; Fks1 hybrids expressing Scedosporium prolificans hot spot 3 confirmed that this substitution imparts RES.
Belief in a Just World, Social Influence and Illness Attributions

Characteristics of individuals and illnesses can both influence receptivity to preventative health messages. We examined whether receptivity to health messages depends on interactions between illness characteristics and dispositional concern for justice. Participants considered the preventability of six illnesses after exposure to a message that manipulated personal responsibility for illness. Paradoxically, participants with strong just world beliefs reported greater preventability for less preventable illnesses, such as brain cancer, when exposed to an unpreventable health message. In parallel, participants with low justice beliefs reported less preventability for lung cancer when exposed to a preventable message. This just world boomerang effect suggests that individual dispositions and illness characteristics can interact in ways that can produce either acquiescence or opposition to persuasive health messages.
The Contribution of Active and Passive Leisure to Children's Well-being

The relation between leisure and well-being, including happiness and self-concept, was examined in 375 children aged 8—12 years. Active leisure (e.g. physical activity) was positively correlated with well-being. Passive leisure (e.g. television and video games) was negatively correlated with well-being. Aspects of active leisure (e.g. the importance of sport to the child and how sports made the child feel) as judged by both parents and children accounted for unique variance in children's wellbeing; passive leisure did not. Similar to previous research on adolescents and adults, active leisure activities were related to children's well-being.
Neutrino Dispersion Relations from a Dark Energy Model

We consider a model in which the neutrino is kinetically coupled to a scalar field and study its implications for environmentally dependent neutrino velocities. Following the usual effective field theory logic, this coupling is expected to arise in neutrino dark energy models. It leads to a Lorentz violation in the neutrino sector. The coupling of the scalar field to the stress tensor of the Earth yields terrestrial neutrino dispersion relations distinct from those in interstellar space.
Affective Forecasting and Advance Care Planning

That sicker people evaluate quality of life in future health status more positively, compared to healthier people, is viewed as an instance of affective forecasting error and explained by Prospect Theory, which holds that two prospects (poor health vs death) are more distinguishable when they are imminent than when distant. In a sample of 230 elderly people, we tested whether life in nine health scenarios would be more acceptable to less healthy individuals than to healthier ones. An interaction between current health status and health scenario supported the relative acceptability of poor-health prospects to sicker individuals, confirming the hypothesis.
Biochemical Analysis of the Role of G118R-Linked Dolutegravir Drug Resistance Substitutions in HIV-1 Integrase

ABSTRACT Drug resistance mutations (DRMs) have been reported for all currently approved anti-HIV drugs, including the latest integrase strand transfer inhibitors (INSTIs). We previously used the new INSTI dolutegravir (DTG) to select a G118R integrase resistance substitution in tissue culture and also showed that secondary substitutions emerged at positions H51Y and E138K. Now, we have characterized the impact of the G118R substitution, alone or in combination with either H51Y or E138K, on 3′ processing and integrase strand transfer activity. The results show that G118R primarily impacted the strand transfer step of integration by diminishing the ability of integrase-long terminal repeat (LTR) complexes to bind target DNA. The addition of H51Y and E138K to G118R partially restored strand transfer activity by modulating the formation of integrase-LTR complexes through increasing LTR DNA affinity and total DNA binding, respectively. This unique mechanism, in which one function of HIV integrase partially compensates for the defect in another function, has not been previously reported. The G118R substitution resulted in low-level resistance to DTG, raltegravir (RAL), and elvitegravir (EVG). The addition of either of H51Y or E138K to G118R did not enhance resistance to DTG, RAL, or EVG. Homology modeling provided insight into the mechanism of resistance conferred by G118R as well as the effects of H51Y or E138K on enzyme activity. The G118R substitution therefore represents a potential avenue for resistance to DTG, similar to that previously described for the R263K substitution. For both pathways, secondary substitutions can lead to either diminished integrase activity and/or increased INSTI susceptibility.
Characterization of the Proliferating Cell Nuclear Antigen of Leishmania donovani Clinical Isolates and Its Association with Antimony Resistance

ABSTRACT Previously, through a proteomic analysis, proliferating cell nuclear antigen (PCNA) was found to be overexpressed in the sodium antimony gluconate (SAG)-resistant clinical isolate compared to that in the SAG-sensitive clinical isolate of Leishmania donovani. The present study was designed to explore the potential role of the PCNA protein in SAG resistance in L. donovani. For this purpose, the protein was cloned, overexpressed, purified, and modeled. Western blot (WB) and real-time PCR (RT-PCR) analyses confirmed that PCNA was overexpressed by ≥3-fold in the log phase, stationary phase, and peanut agglutinin isolated procyclic and metacyclic stages of the promastigote form and by ∼5-fold in the amastigote form of the SAG-resistant isolate compared to that in the SAG-sensitive isolate. L. donovani PCNA (LdPCNA) was overexpressed as a green fluorescent protein (GFP) fusion protein in a SAG-sensitive clinical isolate of L. donovani, and modulation of the sensitivities of the transfectants to pentavalent antimonial (SbV) and trivalent antimonial (SbIII) drugs was assessed in vitro against promastigotes and intracellular (J774A.1 cell line) amastigotes, respectively. Overexpression of LdPCNA in the SAG-sensitive isolate resulted in an increase in the 50% inhibitory concentrations (IC50) of SbV (from 41.2 ± 0.6 μg/ml to 66.5 ± 3.9 μg/ml) and SbIII (from 24.0 ± 0.3 μg/ml to 43.4 ± 1.8 μg/ml). Moreover, PCNA-overexpressing promastigote transfectants exhibited less DNA fragmentation compared to that of wild-type SAG-sensitive parasites upon SbIII treatment. In addition, SAG-induced nitric oxide (NO) production was found to be significantly inhibited in the macrophages infected with the transfectants compared with that in wild-type SAG-sensitive parasites. Consequently, we infer that LdPCNA has a significant role in SAG resistance in L. donovani clinical isolates, which warrants detailed investigations regarding its mechanism.
Reduced Artemisinin Susceptibility of Plasmodium falciparum Ring Stages in Western Cambodia

ABSTRACT The declining efficacy of artemisinin derivatives against Plasmodium falciparum in western Cambodia is a major concern. The knowledge gap in the understanding of the mechanisms involved hampers designing monitoring tools. Here, we culture-adapted 20 isolates from Pailin and Ratanakiri (areas of artemisinin resistance and susceptibility in western and eastern Cambodia, respectively) and studied their in vitro response to dihydroartemisinin. No significant difference between the two sets of isolates was observed in the classical isotopic test. However, a 6-h pulse exposure to 700 nM dihydroartemisinin (ring-stage survival assay -RSA]) revealed a clear-cut geographic dichotomy. The survival rate of exposed ring-stage parasites (ring stages) was 17-fold higher in isolates from Pailin (median, 13.5%) than in those from Ratanakiri (median, 0.8%), while exposed mature stages were equally and highly susceptible (0.6% and 0.7%, respectively). Ring stages survived drug exposure by cell cycle arrest and resumed growth upon drug withdrawal. The reduced susceptibility to artemisinin in Pailin appears to be associated with an altered in vitro phenotype of ring stages from Pailin in the RSA.
Measuring Rebelliousness and Predicting Health Behaviour and Outcomes

The Social Reactivity Scale is a questionnaire measure of individual differences in rebelliousness. The associations between rebelliousness, health behaviours and health outcomes were examined in two Dutch samples by means of cross-sectional survey data. We found moderate support for the reliability and construct validity of the scale. Findings were suggestive of rebelliousness, first, being associated with low control beliefs, second, being related to hostility and, third, also heightening the risk of engaging in unhealthy behaviours and that of poor health (perhaps through deliberately rejecting health education messages). Findings thus contribute to the ongoing emergence of an empirically viable theoretical construct.
A Multilevel Transformerless Inverter Employing Ground Connection Between PV Negative Terminal and Grid Neutral Point

For the safe operation of transformerless grid connected photovoltaic (PV) inverters, the issue of common mode (CM) leakage current needs to be addressed carefully. In this paper, a novel multilevel transformerless inverter topology is proposed, which completely eliminates CM leakage current by connecting grid neutral point directly to the PV negative terminal, thereby bypassing the PV stray capacitance. It provides a low-cost solution consisting of only four power switches, two capacitors, and a single filter inductor. As compared to half-bridge topologies, with this inverter a minimum of 27% and maximum of 100% more output voltage is obtained for the same dc-link voltage. The proposed inverter is analyzed in detail and its switching pattern to generate multilevel output while maintaining the capacitor voltage is discussed. Simulations and experiments results confirm the feasibility and good performance of the proposed inverter.
Plant leaf identification using moment invariants & General Regression Neural Network

Living plant identification based on images of leaf is a very challenging task in the field of pattern recognition and computer vision. However, leaf classification is an important component of computerized living plant recognition. The leaf contains important information for plant species identification despite its complexity. The objective of this study is to compare the effectiveness of Zernike Moment Invariant (ZMI), Legendre Moment Invariant (LMI) and Tchebichef Moment Invariant (TMI) features in extracting features from leaf images. Then, the features extracted from the most effective moment invariant technique are classified using the General Regression Neural Network (GRNN). There are two main stages involved in plant leaf identification. The first stage is known as feature extraction process where moment invariant methods are applied. The output of this process is a set of a global vector feature that represents the shape of the leaf images. It is shown that TMI can extract vector feature with Percentage of Absolute Error (PAE) less than 10.38 percent. Therefore, TMI vector feature will be the input to the second stage. The second stage involves classification of leaf images based on the derived feature gained in the previous stage. It is found that the feature vectors enabled the GRNN classifier to achieve 100 percent classification rate. Thus, the finding from this study can provide useful information for developing automated plant classification tools.
Stand-Alone Stretchable Absolute Pressure Sensing System for Industrial Applications

A stand-alone stretchable absolute pressure sensing system has been proposed and demonstrated. This is the first step towards the development of an absolute pressure distribution monitoring system for industrial applications. The pressure sensing network can be fabricated in a cost-effective manner on silicon wafers and utilizes the advantages of microelectromechanical system technology. The sensor network possesses great stretchability, capable of being expanded to 10 000% times its original dimensions. Pressure sensing elements in the network have a sensitivity of about –0.14 mV/V/kPa. In addition, low-noise readout electronics, designed for signal conditioning, power management, and wireless communication, were implemented to realize the stand-alone absolute pressure sensor network. Furthermore, the stand-alone pressure sensing system prototype has been tested by measuring pressure at different locations in real time. Experimental measurements show good agreement with simulations and a commercial reference sensor. The sensor system can measure real-time mapping profiles of absolute pressure and has great potential for a variety of industrial applications.
An investigation of victimization and the clinical course of chronic fatigue syndrome

Medically unexplained syndromes, including chronic fatigue syndrome (CFS), have been associated with victimization in childhood and adulthood. The purpose of this study was to examine the associations of victimization experiences in childhood and adulthood with functional status and illness severity in a sample of patients with CFS using longitudinal data. In the sample of 93 patients with CFS, childhood abuse and neglect had greater impact than adulthood victimization. Overall, victimization experiences in childhood demonstrated modest associations with clinical outcomes in CFS, although several victimization experiences were in the opposite direction of expectations. Victimization predicted worse outcomes, but not worsening outcomes over time.
Answering Patient Questions about the Role Lifestyle Factors Play in Cancer Onset and Recurrence

This qualitative study examined how cancer specialists answer patient questions about what might have caused their cancer. Findings showed that while they were often candid about the role of smoking and drinking in cancer onset and that of diet in cancer recurrence, body weight and exercise were rarely mentioned. Any reluctance to discuss the role of lifestyle factors in cancer onset and recurrence arose from a desire to minimize patient distress, limitations in specialists’ knowledge of the causes of cancer and perceived inadequacy of the available causal explanations when risk factors are multiple and probabilistic.
Knowledge Discovery and Data Mining of Free Text Radiology Reports

Medical Knowledge Discovery and Data Mining (KDD) over text is a promising yet difficult technology for unlocking meaning and uncovering associations in vast clinical text repositories. We report our experience in developing a new text analytic system called MEDAT or Medical Exploratory Data Analysis over Text, which overcomes several problems in text mining. The MEDAT system employs an annotated semantic index with a large number of assertions (propositions). The semantic index is able to capture complex assertions which encapsulate conceptual relationships including their modifiers at a granular level. The index represents semantically equivalent sentences with the same symbols, a necessary component for KDD semantic queries, including semantic Boolean and correlation queries. The graphical user interface enables users to perform complex semantic analysis of the Roentgen corpus, consisting of 594,000 de-identified radiology reports with 4.3 million sentences, without having to learn a programming language. The MEDAT architecture offers a novel framework for text mining in other medical domains.
genegames.org: High-Throughput Access to Biological Knowledge and Reasoning through Online Games

Games are emerging as a powerful organizational and motivational tactic throughout many areas of society. Wherever people have a goal that they are having trouble reaching, be it getting their chores done [1], learning all the functions of Microsoft Visual studio [2], or finishing a 10K [3], many are finding success by posing the required tasks as elements of games. Games can turn small units of work, that alone might seem boring, into fun steps taken towards a meaningful success. In doing so, they can sometimes dramatically increase individuals' chances of reaching their objectives. The process of translating elements of non-game contexts (e.g. science, most traditional work, learning, exercise, etc.) into aspects of games is now known as `gamification'.
Analysis of Ribonucleotide 5′-Triphosphate Analogs as Potential Inhibitors of Zika Virus RNA-Dependent RNA Polymerase by Using Nonradioactive Polymerase Assays

ABSTRACT Zika virus (ZIKV) is an emerging human pathogen that is spreading rapidly through the Americas and has been linked to the development of microcephaly and to a dramatically increased number of Guillain-Barré syndrome cases. Currently, no vaccine or therapeutic options for the prevention or treatment of ZIKV infections exist. In the study described in this report, we expressed, purified, and characterized full-length nonstructural protein 5 (NS5) and the NS5 polymerase domain (NS5pol) of ZIKV RNA-dependent RNA polymerase. Using purified NS5, we developed an in vitro nonradioactive primer extension assay employing a fluorescently labeled primer-template pair. Both purified NS5 and NS5pol can carry out in vitro RNA-dependent RNA synthesis in this assay. Our results show that Mn2+ is required for enzymatic activity, while Mg2+ is not. We found that ZIKV NS5 can utilize single-stranded DNA but not double-stranded DNA as a template or a primer to synthesize RNA. The assay was used to compare the efficiency of incorporation of analog 5′-triphosphates by the ZIKV polymerase and to calculate their discrimination versus that of natural ribonucleotide triphosphates (rNTPs). The 50% inhibitory concentrations for analog rNTPs were determined in an alternative nonradioactive coupled-enzyme assay. We determined that, in general, 2′-C-methyl- and 2′-C-ethynyl-substituted analog 5′-triphosphates were efficiently incorporated by the ZIKV polymerase and were also efficient chain terminators. Derivatives of these molecules may serve as potential antiviral compounds to be developed to combat ZIKV infection. This report provides the first characterization of ZIKV polymerase and demonstrates the utility of in vitro polymerase assays in the identification of potential ZIKV inhibitors.