Miracle-dz/UniFAR-FTUs huggingface dataset:
Are Inter-Beat Intervals From Blood Pressure a Valid Alternative to R-R Intervals for the Multiscale Entropy Analysis of Heart Rate Variability?

When an ECG recording is not available to derive the R-R interval (RRI) series, the heart rate variability (HRV) may be evaluated considering the inter-beat intervals (IBI) from other cardiovascular signals, like the arterial blood pressure (ABP). However, it is unknown how the ABP-derived IBI series quantify the multiscale entropy (MSE) of HRV. Thus our aim is to describe differences between RRI and the ABP-derived IBI in MSE estimates. We recorded the ECG and the finger ABP in 40 volunteers. We derived the RRI series from the ECG (reference IBI) and the series of systolic-systolic intervals (SSI), diastolic-diastolic intervals (DDI), and intervals between maxima of the first- (d'PI) and second- (d“PI) derivative of ABP. For each IBI series, we estimated the MSE at the scale s=1 beat (SampEn), at high-frequency scales (MSE<inf>HF</inf>, for 2≤s≤7 beats) and low-frequency scales (MSE<inf>LF</inf>, for 7<s≤25 beats), comparing estimates from the ABP-derived IBI with the RRI reference. Sampen was substantially overestimated while MSE<inf>HF</inf> and MSE<inf>LF</inf> were underestimated by ABP-derived IBI. The estimates closest to RRI were provided by d'PI (error +11% for SampEn, − 2% for MSE<inf>HF</inf>) and SSI (-2% for MSE<inf>LF</inf>). Thus, the ABP-derived IBI series reflect SampEn components absent in RRI, being d'PI and SSI the closest surrogate for RRI.
Help-seeking for mental health services in Asian American college students: an exploratory qualitative study

Abstract Objective: To understand how the college environment might influence mental health help-seeking in Asian American undergraduates. Participants: Participants were 19 Asian American undergraduates recruited through Facebook. Methods: Participants were interviewed about attitudes toward mental health and mental health help-seeking, and thematic analysis with both inductive and deductive codes was used to analyze interview transcripts. Results: Several factors specific to the college environment encourage students to seek help: social support provided by peers and by campuses, psychological distance from home, and physical distance from home. However, other Asian American-specific factors discourage students from seeking help, such as cultural factors and stigma surrounding mental health issues. Conclusions: The college environment may encourage help-seeking, while Asian American-specific factors may discourage it. Potential interventions based on these findings may increase help-seeking rates by focusing on these encouraging factors in a culturally-sensitive way.
Raddeanin A suppresses lung cancer cell proliferation via induction of apoptosis and increased production of ROS.

At present, in vitro cell experiments have confirmed that RaddeaninA can effectively inhibit the proliferation of some tumor cells, but the effect of RaddeaninA on lung cancer cells has not been observed. Therefore, this study explored its effect on lung cancer cells and its mechanism of action. Human lung cancer cell lines were treated with serum-free medium and varied concentrations of Raddeanin A. Cell proliferation and apoptosis were determined using MTT, and flow cytometric assays, respectively. The intracellular level of ROS was determined using DCFH-DA assay. Protein and mRNA expressions of bax, bcl-2 and cyt c were measured using Western blotting and qRT-PCR. RaddeaninA treatment can promote PC-9 cell apoptosis in a time and dose-dependent manner (p<0.05). Treatment of PC-9 cells with Raddeanin significantly and dose-dependently increased the activities of caspase-9 and caspase-3 (p<0.05), and led to significant and dose-dependent increases in ROS levels (p<0.05). Treatment of PC-9 cells with Raddeanin A led to significant and dose-dependent decreases in mitochondrial membrane potential (p<0.05). It significantly and dose-dependently upregulated bax mRNA and protein expressions, but down-regulated bcl-2 mRNA and protein expressions significantly and dose-dependently (p<0.05). On the other hand, Raddeanin significantly and dose-dependently down-regulated cytoplasmic bax protein expression, while upregulating cyt c expression (p<0.05). Similarly, bax protein expression was significantly and dose-dependently upregulated in mitochondria, but the corresponding cyt c expression was significantly and dose-dependently down-regulated (p<0.05). Raddeanin A is a potential and effective lung cancer chemotherapy drug, which can induce lung cancer cell apoptosis and inhibit proliferation.
Integrating Circulating Biomarkers in the Immune Checkpoint Inhibitor Treatment in Lung Cancer

Simple Summary Immune checkpoint inhibitors (ICI) are now a cornerstone of treatment for non-small cell lung cancer (NSCLC). Despite reporting tremendous results for some patients, ICI efficacy remains reserved to a subgroup that is not yet fully characterized. Tissue based assays, such as Programmed cell death protein 1 (PD-L1) expression may enrich the responder population, but this biomarker is not always available or reliable, as responses have been observed in patients with negative PD-L1. Blood markers are hoped to be easier to access and follow, and to give an insight on patient’s immune status and tumor as well. To date, several papers have been looking for circulating biomarkers that are focused on tumor cells or host specific or general immunity in NSCLC treated with ICI. In this article, we review these circulating biomarkers in peculiar circulating immune cell, tumor related cell and soluble systemic marker. We describe the available data and comment on the technical requirements and limits of these promising techniques. Abstract Immune checkpoint inhibitors are now a cornerstone of treatment for non-small cell lung cancer (NSCLC). Tissue-based assays, such as Programmed cell death protein 1 (PD-L1) expression or mismatch repair deficiency/microsatellite instability (MMRD/MSI) status, are approved as treatment drivers in various settings, and represent the main field of research in biomarkers for immunotherapy. Nonetheless, responses have been observed in patients with negative PD-L1 or low tumor mutational burden. Some aspects of biomarker use remain poorly understood and sub-optimal, in particular tumoral heterogeneity, time-evolving sampling, and the ability to detect patients who are unlikely to respond. Moreover, tumor biopsies offer little insight into the host’s immune status. Circulating biomarkers offer an alternative non-invasive solution to address these pitfalls. Here, we summarize current knowledge on circulating biomarkers while using liquid biopsies in patients with lung cancer who receive treatment with immune checkpoint inhibitors, in terms of their potential as being predictive of outcome as well as their role in monitoring ongoing treatment. We address host biomarkers, notably circulating immune cells and soluble systemic immune and inflammatory markers, and also review tumor markers, including blood-based tumor mutational burden, circulating tumor cells, and circulating tumor DNA. Technical requirements are discussed along with the current limitations that are associated with these promising biomarkers.
STUDY OF THE VIRAL PHYLOGENIC DIAGNOSIS OF AVIAN INFLUENZA DISEASE IN BROILER CHICKENS IN BABYLON PROVINCE, IRAQ

Avian influenza (AI) disease are infectious, highly contagious viral diseases infected the respiratory and digestive system that cause neurological signs and sudden death combined with high mortality (90%) in poultry industry. Assessment of viral phylogenic diagnosis of avian influenza disease in broiler chickens in Babylon province. Identification of AIV in Samples (lung, trachea, spleen and intestine) were done by indirect ELISA test and were conducted to identify (AIV) by using 18 samples of specific serum from infected broiler chick. The molecular method (qRT-PCR) test also confirmed for 7 samples infected with AIV (H9N2). The nucleotide sequencing and translation results for cleavage site of HA-gene for AIV showed that all these 2 samples are low pathogenic because they don’t contain multiple basic amino acids at cleavage site and the sequencing of all these strains at cleavage site is (PAKSSRGLF). Isolated strains of AIV isolated strains were registered in NCBI is begun by accession number (A1908158.002) and ends (A1908158.003).
Religiosity, Acculturation, and Preterm Birth in Mexican-Origin Women: A Pilot Study

Introduction Explanations for racial disparities in preterm birth (PTB) are elusive, especially when comparing high rates in some racial groups with low rates in Mexican-immigrant women. The purpose of this study was to examine potential protective factors against PTB such as religiosity and acculturation. Methods This study was a prospective investigation of Mexican- and U.S.-born pregnant women. Women were recruited from a low-income-serving prenatal clinic in Texas. Survey instruments included socioeconomic variables, acculturation, and religiosity/spirituality (R/S). Logistic regression was used to examine the associations between acculturation, religiosity, and PTB. Because of the low prevalence of PTB in our sample, we were not able to adjust for confounding characteristics. Results Ninety-one low-income women, mostly Mexican immigrants, participated in the study. PTB in our sample was lower than the national average in the United States (5.5% vs. 9.9%) and was positively but moderately associated with high R/S. R/S scores were high, particularly for frequency of attendance, prayer, and religious coping. Women with lower acculturation had higher scores on the religiosity measures. Conclusion Further research is needed with a larger sample to include other ethnic and racial minorities to more fully understand the relationships between acculturation, religiosity, and PTB.
The farther the better: Investigating how distance from human self affects the propensity of a peptide to be presented on cell surface by MHC class I molecules, the case of Trypanosoma cruzi

More than twenty years ago the reverse vaccinology paradigm came to light trying to design new vaccines based on the analysis of genomic information in order to select those pathogen peptides able to trigger an immune response. In this context, focusing on the proteome of Trypanosoma cruzi, we investigated the link between the probabilities for pathogen peptides to be presented on a cell surface and their distance from human self. We found a reasonable but, as far as we know, undiscovered property: the farther the distance between a peptide and the human-self the higher the probability for that peptide to be presented on a cell surface. We also found that the most distant peptides from human self bind, on average, a broader collection of HLAs than expected, implying a potential immunological role in a large portion of individuals. Finally, introducing a novel quantitative indicator for a peptide to measure its potential immunological role, we proposed a pool of peptides that could be potential epitopes and that can be suitable for experimental testing. The software to compute peptide classes according to the distance from human self is free available at http://www.iasi. cnr.it/~dsantoni/nullomers.
Evaluation of analytical instrumentation. Part XXIII. Instrumentation portable X-ray fluorescence spectrometry

The reports of this series tabulate a number of features of analytical instruments that should be considered when making comparison between various systems. Scoring these features in a rational manner allows a scientific comparison to be made between instruments as an aid to selection. This is the XXIII report of the series and deals with instrumentation for portable X-ray fluorescence spectrometry.
Machine learning in agriculture: from silos to marketplaces

The increasing global population combined with climate change present a major challenge for agriculture. Most crops have been bred to perform in specific environments, and with the time required to produce new varieties, it is unlikely that breeders will be able to adapt varieties to the changing climate (Abberton et al., 2016). There is an urgent need to develop new approaches to accelerate the production of high performing resilient crop varieties. Crop breeding has seen many changes in recent decades, from the application of molecular markers, through to genetically modified, and more recently, genome edited crops (Scheben et al., 2017). However, these approaches are often limited by our lack of understanding of the genomic basis for complex traits, even with the deluge of data being generated by new genome sequencing and phenomics technologies. New approaches are required to translate this explosion of data into improved crop varieties.
Nanoparticles Supported on Sub‐Nanometer Oxide Films: Scaling Model Systems to Bulk Materials

Abstract Ultrathin layers of oxides deposited on atomically flat metal surfaces have been shown to significantly influence the electronic structure of the underlying metal, which in turn alters the catalytic performance. Upscaling of the specifically designed architectures as required for technical utilization of the effect has yet not been achieved. Here, we apply liquid crystalline phases of fluorohectorite nanosheets to fabricate such architectures in bulk. Synthetic sodium fluorohectorite, a layered silicate, when immersed into water spontaneously and repulsively swells to produce nematic suspensions of individual negatively charged nanosheets separated to more than 60 nm, while retaining parallel orientation. Into these galleries oppositely charged palladium nanoparticles were intercalated whereupon the galleries collapse. Individual and separated Pd nanoparticles were thus captured and sandwiched between nanosheets. As suggested by the model systems, the resulting catalyst performed better in the oxidation of carbon monoxide than the same Pd nanoparticles supported on external surfaces of hectorite or on a conventional Al2O3 support. XPS confirmed a shift of Pd 3d electrons to higher energies upon coverage of Pd nanoparticles with nanosheets to which we attribute the improved catalytic performance. DFT calculations showed increasing positive charge on Pd weakened CO adsorption and this way damped CO poisoning.
Copro-molecular diagnosis of the Toxoplasmatinae subfamily in dog and cat populations in northern Iran

OBJECTIVES The oocysts of the Toxoplasmatinae subfamily (Neospora caninum, Hammondia hammondi and H. heydorni, and Besnoitia besnoiti) are morphologically similar to Toxoplasma gondii, and indistinguishable from each other. This study investigated the prevalence of the Toxoplasmatinae subfamily in dog and cat fecal samples using a nested polymerase chain reaction method. METHODS Overall, 200 fecal samples from domestic dogs (n=120) and cats (n=80) were collected from 15 farms in northern Iran. The samples were homogenized in 2.5% potassium dichromate solution and subsequently concentrated with sucrose solution. DNA was extracted from samples using a genomic DNA kit. Specific primers and the 18S rDNA gene were used to screen and detect all Toxoplasmatinae oocysts. RESULTS Overall, 2.5% (3 of 120) and 22.5% (18 of 80) of the fecal samples collected from dogs and cats were infected with Toxoplasmatinae. In dogs, 2 samples were positive for N. caninum and 1 sample was positive for T. gondii. In cats, all 18 positive samples belonged to T. gondii. No contamination with H. heydorni was observed in dog fecal samples or H. hammondi and B. besnoiti in cat fecal samples. A phylogenetic analysis revealed that the T. gondii (cat) and N. caninum (dog) found had similarities with parasites reported from other regions of the world. CONCLUSIONS This is the first study to provide data on the epidemiology of Toxoplasmatinae oocysts in Iran. The findings suggest that public-health monitoring for the effective control of feces from cats and dogs and improved pet hygiene habits are needed.
Focus on DNA Glycosylases—A Set of Tightly Regulated Enzymes with a High Potential as Anticancer Drug Targets

Cancer is the second leading cause of death with tens of millions of people diagnosed with cancer every year around the world. Most radio- and chemotherapies aim to eliminate cancer cells, notably by causing severe damage to the DNA. However, efficient repair of such damage represents a common mechanism of resistance to initially effective cytotoxic agents. Thus, development of new generation anticancer drugs that target DNA repair pathways, and more particularly the base excision repair (BER) pathway that is responsible for removal of damaged bases, is of growing interest. The BER pathway is initiated by a set of enzymes known as DNA glycosylases. Unlike several downstream BER enzymes, DNA glycosylases have so far received little attention and the development of specific inhibitors of these enzymes has been lagging. Yet, dysregulation of DNA glycosylases is also known to play a central role in numerous cancers and at different stages of the disease, and thus inhibiting DNA glycosylases is now considered a valid strategy to eliminate cancer cells. This review provides a detailed overview of the activities of DNA glycosylases in normal and cancer cells, their modes of regulation, and their potential as anticancer drug targets.
Drivers of assemblage-wide calling activity in tropical anurans and the role of temporal resolution.

1. Temporal scale in animal communities is often associated with seasonality, despite the large variation in species activity during a diel cycle. A gap thus remains in understanding the dynamics of short-term activity in animal communities. 2. Here we assessed calling activity of tropical anurans and addressed how species composition varied during night activity in assemblages along gradients of local and landscape environmental heterogeneity. 3. We investigated 39 anuran assemblages in the Pantanal wetlands (Brazil) with passive acoustic monitoring during the peak of one breeding season and first determined changes in species composition between night periods (early, mid, and late) using two temporal resolutions (1-hour and 3-hour intervals). Then, we addressed the role of habitat structure (local and landscape heterogeneity variables from field-based and remote sensing metrics) and ecological context (species richness and phylogenetic relatedness) in determining changes in species composition (i) between night periods and (ii) across days. 4. Nocturnal calling activity of anuran assemblages varied more within the 1-hour resolution than the 3-hour resolution. Differences in species composition between early and late-night periods were related to local habitat structure and phylogenetic relatedness, while a low variation in compositional changes across days was associated with low-heterogeneous landscapes. None of these relationships were observed using the coarser temporal resolution (3-hour). 5. Our findings on the variation of calling activity in tropical anuran assemblages suggest potential trades-off mediated by fine-temporal partitioning. Local and landscape heterogeneity may provide conditions for spatial partitioning, while the relatedness among co-signaling species provides cues on the ecological overlap of species with similar requirements. These relationships suggest a role of niche dimensional complementarity on the structuring of these anuran assemblages over fine temporal scales. We argue that fine-temporal differences between species in breeding activity can influence the outcome of species interaction and that addressing temporal scaling issues can improve our understanding of dynamics of animal communities.
Graphene H-Waveguide for Terahertz Lasing Applications: Electromagnetic Quasi-Linear Theory

A novel graphene H-waveguide is proposed for active terahertz components. A graphene film illuminated by strong pumping light shorts the parallel conductor plates. The terahertz modes propagating along this film are amplified at certain conditions. A rigorous electromagnetic (EM) quasi-linear method of analytical calculations of TEy and TMy eigenmodes is used in this paper to select these conditions. Among them is the use of bound TEy modes interacting with graphene plasmons at frequencies of negative graphene resistance, minimizing conductor loss associated with parallel plates, and excluding the current-crowding effect from the waveguide design. The limitations of the used theory are considered, and the applications of this waveguide are proposed.
Highly Branched Neo-Fructans (Agavins) Attenuate Metabolic Endotoxemia and Low-Grade Inflammation in Association with Gut Microbiota Modulation on High-Fat Diet-Fed Mice

Highly branched neo-fructans (agavins) are natural prebiotics found in Agave plants, with a large capacity to mitigate the development of obesity and metabolic syndrome. Here, we investigated the impact of agavins intake on gut microbiota modulation and their metabolites as well as their effect on metabolic endotoxemia and low-grade inflammation in mice fed high-fat diet. Mice were fed with a standard diet (ST) and high-fat diet (HF) alone or plus an agavins supplement (HF+A) for ten weeks. Gut microbiota composition, fecal metabolite profiles, lipopolysaccharides (LPS), pro-inflammatory cytokines, and systemic effects were analyzed. Agavins intake induced substantial changes in gut microbiota composition, enriching Bacteroides, Parabacteroides, Prevotella, Allobaculum, and Akkermansia genus (LDA > 3.0). l-leucine, l-valine, uracil, thymine, and some fatty acids were identified as possible biomarkers for this prebiotic supplement. As novel findings, agavins supplementation significantly decreased LPS and pro-inflammatory (IL-1α, IL-1β, and TNF-α; p < 0.05) cytokines levels in portal vein. In addition, lipid droplets content in the liver and adipocytes size also decreased with agavins consumption. In conclusion, agavins supplementation mitigate metabolic endotoxemia and low-grade inflammation in association with gut microbiota regulation and their metabolic products, thus inducing beneficial responses on metabolic disorders in high-fat diet-fed mice.