Miracle-dz/UniFAR-FTUs huggingface dataset:
Homodimerization of Marek’s Disease Virus-Encoded Meq Protein Is Not Sufﬁcient for Transformation of Lymphocytes in Chickens (cid:1)

Marek’s disease virus (MDV), the etiologic agent of Marek’s disease, is a potent oncogenic herpesvirus. MDV is highly contagious and elicits a rapid onset of malignant T-cell lymphomas in chickens within several weeks after infection. MDV genome codes an oncoprotein, Meq, which shares resemblance with the Jun/Fos family of bZIP transcription factors. Similar to Jun, the leucine zipper region of Meq allows the formation of homo-and heterodimers. Meq homo- and heterodimers have different DNA binding afﬁnities and transcriptional activity; therefore, they may differentially regulate transcription of viral and cellular genes. In this study we investigated the role of Meq homodimers in the pathogenicity of MDV by generating a chimeric meq gene, which contains the leucine zipper region of the yeast transcription factor GCN4 ( meqGCN ). A recombinant virus (rMd5-MeqGCN) containing the chimeric meqGCN gene in place of parental meq was generated with overlapping cosmid clones of Md5, a very virulent MDV strain. The rMd5-MeqGCN virus replicated in vitro and in vivo but was unable to transform T cells in infected chickens. These data provide the ﬁrst in vivo evidence that Meq homodimers are not sufﬁcient for MDV-induced transformation. generated EcoQ fragment (EcoQ-MeqGCN) the mutated meq leucine zipper (MeqGCN) into cosmids A6 (cid:2) EcoQ and SN5 (cid:2) EcoQ, using RARE. The EcoRI site of cosmids A6 (cid:2) EcoQ and SN5 (cid:2) EcoQ was protected during the methylation reaction using recA and primer SR1130 (5 (cid:3) -GAA TCG G-3 (cid:3) to EcoRI restriction digestion to linearize the cosmids, and subsequently ligated to the EcoQ-MeqGCN fragment, yielding cosmids SN5-MeqGCN and A6-MeqGCN. After ligation and transduction, clones containing the Eco-MeqGCN fragment in the correct ori- entation were identiﬁed by PCR, and selected positive colonies were sequenced across the junction regions. The integrity of the SN5-MeqGCN and A6- MeqGCN cosmids was conﬁrmed by evaluating their restriction digestion pat-tern. Transfections. Parental and mutant cosmid DNA were digested with NotI to release the viral insert and puriﬁed by phenol-chloroform extraction and ethanol precipitation before transfection. To generate rMd5 and rMd5-MeqGCN, 500 ng of cosmids P89, SN16, B40, SN5, and A6 or P89, SN16, B40, SN5-MeqGCN, and A6-MeqGCN, respectively, were used to transfect 1.2 (cid:4) 10 6 DEF in 60-mm dishes by the calcium phosphate procedure. At 5 days posttransfection, cells were trypsin treated, seeded onto a 100-mm dish, and monitored for cytopathic effects. Viral stocks of recovered viruses were subsequently made in DEF for further analysis.
Genetically encoded FRET sensor to detect double- stranded RNA during viral infection

6 ZUSAMMENFASSUNG . 7 INTRODUCTION . 8 Motivation and scientific background . 8 RNA-viruses . 9 Hepatitis C Virus . 12 Cellular response to the infection of RNA viruses . 15 PKR structure and biology . 18 eIF2α structure and biology . 21 Genetically encoded FRET sensors . 22 Aims and sensor design approaches . 25 METHODS . 29 Cloning . 29 Cell culture . 31 Transfection . 32 Native cell lysis . 32 Fluorimetry . 33 In vitro analysis of the FRET sensor library . 35
CALL PAPERS Cardiovascular and Cerebrovascular Aging–New Mechanisms and Insights Geometry is a major determinant of ﬂow reversal in proximal aorta

E. Geometry is a major determinant of ﬂow reversal in proximal aorta. aim of this study is to quantify aortic backward ﬂow (BF) using phase-contrast cardiovascular magnetic resonance (PC-CMR) and to study its associations with age, indexes of arterial stiffness, and geometry. Although PC-CMR blood ﬂow studies showed a simultaneous presence of BF and forward ﬂow (FF) in the ascending aorta (AA), the relationship between aortic ﬂows and aging as well as arterial stiffness and geometry in healthy volunteers has never been reported. We studied 96 healthy subjects [47 women, 39 (cid:2) 15 yr old (19–79 yr)]. Aortic stiffness [arch pulse wave velocity (PWV AO ), AA distensibility], geometry (AA diameter and arch length), and parameters related to AA BF and FF (volumes, peaks, and onset times) were estimated from CMR. Applanation tonometry carotid-femoral pulse-wave velocity (PWV CF ), carotid augmentation index, and time to return of the reﬂected pressure wave were assessed. Whereas FF parameters remained unchanged, BF onset time shortened signiﬁcantly ( R 2 (cid:3) 0.18, P (cid:4) 0.0001) and BF volume and BF-to-FF peaks ratio increased signiﬁcantly ( R 2 (cid:3) 0.38 and R 2 (cid:3) 0.44, respectively, P (cid:4) 0.0001) with aging. These two latter BF indexes were also related to stiffness indexes (PWV CF , R 2 (cid:5) 0.30; PWV AO , R 2 (cid:5) 0.24; and distensibility, R 2 (cid:5) 0.20, P (cid:4) 0.001), augmentation index ( R 2 (cid:5) 0.20, P (cid:4) 0.001), and aortic geometry (AA diameter, R 2 (cid:5) 0.58; and arch length, R 2 (cid:5) 0.31, P (cid:4) 0.001). In multivariate analysis, aortic diameter was the strongest independent correlate of BF beyond age effect. In conclusion, AA BF estimated using PC-CMR increased signiﬁcantly in terms of magnitude and volume and appeared earlier with aging and was mostly determined by aortic geometry. Thus BF indexes could be relevant markers of subclinical arterial wall alterations.
Assessment of Postnatal Women's Satisfaction Regarding the Nursing Care in the Obstetric Hospitals in Mosul City

Background: the satisfaction of women has considered as a significant pointer of the services quality that provided by the professional of health care. Postnatal women satisfaction upon the services and care of nursing is useful and important for the agencies of health care because nurses present the majority of health care and provide the nursing care 24 hours a day for women. Aim of study: The aim of the current study is to assess the postnatal women's satisfaction regarding the nursing care in the obstetric hospitals in Mosul city. Methodology: A descriptive design was carried-out from 15th of November 2013 to 15th April 2014, and conducted in two hospitals in the Mosul city (Al-Batool and Al-Khanssa) teaching hospitals. A random sample was used for selecting (300) participation. The questionnaire research is corrected by (11) of college of nursing experts. The reliability of the questionnaire estimated through coefficient correlation method which indicates 0.89. The data were analyzed using a descriptive and inferential statistical analysis. Conclusions: the study concludes that it is generally no satisfaction of postnatal women in several aspects of nursing care in gynecological hospitals in Mosul city. Recommendation: the current study recommends that program and training courses should be done for nurses to advance their information and training aspect and improve their communication skills with the patients to present a significant level of nursing care to improve the patients satisfaction in general. The outcomes of the present study will send to the Nineveh Health Directorate to get them in view.
L p -strong convergence of the averaging principle for slow-fast SPDEs with jumps (cid:73)

The averaging principle is an important method to extract eﬀective macro-scopic dynamic from complex systems with slow component and fast component. This paper concerns the L p -strong convergence of the averaging principle for two-time-scales stochastic partial diﬀerential equations (SPDEs) driven by Wiener processes and Poisson jumps. To achieve this, a key step is to show the existence for an invariant measure with exponentially ergodic property for the fast equation, where the dissipative conditions are needed. Furthermore, it is shown that under suitable assumptions the slow component L p -strongly converges to the solution of the averaged equation. The rate of the convergence is also obtained as a byproduct.
A Case for Inverted Indices in In-Memory Databases

—Recent database research has focused on in-memory databases, which can be used in mixed workload scenarios, enabling OLTP and OLAP queries on the same database engine. These compressed column-oriented database systems use a differential store concept to enable fast inserting and require a merge process to compact the data periodically in a compressed main partition that is changed by the merge process only. This characteristic feature calls for a re-evaluation of the performance of inverted indices. In this paper we present a use case for an inverted index in a column-oriented in-memory database system to reduce the total costs of query processing in a mixed workload environment. We evaluate the beneﬁts and drawbacks of using the index structure to answer queries and the costs of maintaining the index, especially during the merge process. An analytical model is introduced to compute the theoretical cost of index scans. Furthermore a comparison between different index maintenance strategies is presented. The theoretical ﬁndings are veriﬁed in a prototypic implementation within the HYRISE database system. Our contributions are an analytical framework to evaluate the beneﬁt of an inverted index during query execution, the veriﬁcation in an in-memory database system, and an evaluation of different index maintenance strategies. From the presented ﬁndings we conclude that indexing can be an efﬁcient instrument to meet the performance requirements in a mixed-workload and main memory-based environment.
PRBM_A_330827 1543..1554

1Institute of Arts and Humanities, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China; 2Institute of Psychological and Behavioral Science, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China; 3School of Humanities, Shanghai Institute of Technology, Shanghai, 201418, People’s Republic of China Purpose: WeChat is China’ biggest social media platform. In addition to instant messaging, it has a great many useful functions that can help Chinese citizens adapt to an increasingly digitized society. This study aimed to examine the effect of WeChat use on the subjective well-being (SWB) of Chinese older adults living alone, and how the effect could occur. Participants and Methods: A total of 415 Chinese older adults living alone participated in the survey. Participants filled out questionnaires (scales) measuring their SWB, proficiency in WeChat use, social activity levels and self-esteem respectively. Mean scores of SWB were used to determine the difference in SWB between the users and non-users, and correlation test was performed to find out the association between proficiency in WeChat use and SWB among the users. Results: The mean score of WeChat users was 34.68 (SD = 7.301), significantly higher than that of the non-users, which was 32.73 (SD = 7.168) (t = 2.741, p = 0.006). Among the users, the more proficient in WeChat use one was, the higher their SWB score (r = 0.458, p < 0.01). The results combined to demonstrate that WeChat use has a positive effect on the SWB of Chinese older adults living alone. The findings also showed that the increase in SWB was obtained through the mediation of intergenerational support and social activities. Conclusion: This study suggests that WeChat use by Chinese older adults living alone could increase their intergenerational support and social activities; the increased intergenerational support and social activities then enhance the SWB of Chinese older adults living alone.
B035 Application of Spectral Decomposition and Ant Tracking to Fractured Carbonate Reservoirs

SUMMARY This paper uses the integrated application of ant tracking and spectral decomposition to detect minor faults and fractures based on the VSP-driven processing seismic data in fractured carbonate reservoirs. A new method is developed to improve imaging of faults and fractures using “discrete frequency ant tracking data”. Discrete frequency ant tracking data is computed using discrete frequency phase data obtained from spectral decomposition. Discrete frequency ant tracking data is more effective at detecting faults and fractures than full spectrum data. The different discrete frequency ant tracking attribute maps show that the high-frequency ant tracking data is more effective at detecting faults and fractures. When compared with the full spectrum data, the reconstructed ant tracking data produces more detailed images of faults and fractures. The results of our case study shows, this has the potential to be a highly effective and very valuable seismic method.
The Association of Notch2 and NF- (cid:2) B Accelerates RANKL-Induced Osteoclastogenesis (cid:1)

Notch signaling plays a key role in various cell differentiation processes including bone homeostasis. However, the speciﬁc involvement of Notch in regulating osteoclastogenesis is still controversial. In the present study, we show that RANKL induces expression of Jagged1 and Notch2 in bone marrow macrophages during osteoclast differentiation. Suppression of Notch signaling by a selective (cid:1) -secretase inhibitor or Notch2 short hairpin RNA suppresses RANKL-induced osteoclastogenesis. In contrast, induction of Notch signaling by Jagged1 or by ectopic expression of intracellular Notch2 enhances NFATc1 promoter activity and expression and promotes osteoclastogenesis. Finally, we found that Notch2 and p65 interact in the nuclei of RANKL- stimulated cells and that both proteins are recruited to the NFATc1 promoter, driving its expression. Taken together, our results show a new molecular cross talk between Notch and NF- (cid:2) tetracycline-regulated pENTR/ transfecting stably pcDNA6/TR. Transfection g/ml blasticidin (cid:5) g/ml zeocin selection. cell attenuate Notch1 RT-PCR immuno- blotting.
Objects, Types and Modal Logics

In this paper we present a modal logic for describing properties of terms in the object calculus of Abadi and Cardelli [AC96]. The logic is essentially the modal mu-calculus of [Koz83]. The fragment allows us to express the temporal modalities of the logic CTL [BAMP83]. We investigate the connection between the type system Ob_1 mu-calculus, providing a translation of types into modal formulae and an ordering on formulae that is sound w.r. t. to the subtype ordering of Ob_1<:mu.
The Use of aPCC in Prophylaxis in a Patient with Hemophilia A with Inhibitor - Case Report

The development of factor VIII inhibitor is a major complication of hemophilia A treatment. The treatment of choice for patients with inhibitors is immune tolerance induction. This is based on a regular administration of preparations containing factor VIII. As a result, the inhibitor is eliminated from patient’s blood. We present the case of patient who is suffering from severe hemophilia A complicated by factor VIII inhibitors. The attempt to induce immunotolerance was ineffective. The patient started a bypassing therapy with activated prothrombin complex concentrate and recombinant activated factor VII on demand. He had several dozens of subcutaneous hemorrhages and joint bleeds that resulted in hemophilic arthropathy. The patient switched to aPCC prophylaxis that resulted in reduced frequency and intensity of bleedings. As a consequence of highly effective treatment and intensive rehabilitation, the quality of his life has significantly improved.
PERFORMANCE EVALUATION OF SELECTED MUTUAL FUND EQUITY GROWTH SCHEMES IN INDIA: WITH SPECIAL REFERENCE TO INFRA, TECHNOLOGY & BANKING SECTOR

Mutual funds are an essential component of the stock market, which in the latest years has become an investment route for many investors. Mutual funds provide a diverse class of investors with investment choices under varying rates of risk and return. The current research aims to assess the efficiency of Indian sectoral mutual fund equity growth schemes in India (Infrastructure, Banking and Technology) through various performance measurement models like Sharpe, Treynor and Jensen for the period 2010 to 2019. For the present study, 91-day T-BiIls return has been taken as a risk-free rate i.e., 7.46 percent p.a. The objective of the study is to analyze the performance of sectoral mutual fund schemes and understand the relationship between sector scheme return and the benchmark return. Kendall's Coefficient of Concordance and Karl Pearson's Coefficient of Correlation have been used. The research result reveals that all chosen schemes performed better than the benchmark return.
Aquaporin 7 Is a (cid:2) -Cell Protein and Regulator of Intraislet Glycerol Content and Glycerol Kinase Activity, (cid:2) -Cell Mass, and Insulin Production and Secretion (cid:1) †

To investigate if intracellular glycerol content plays a role in the regulation of insulin secretion in pancreatic (cid:1) cells, we studied the expression of the glycerol channels, or aquaglyceroporins, encoded by the aquaporin 3 ( Aqp3 ), Aqp7 , and Aqp9 genes in mouse islets. We found expression of Aqp7 only, not that of Aqp3 or Aqp9 , in the endocrine pancreas at both the mRNA (by reverse transcription-PCR) and protein (by immunohistochem- istry) levels. Immunohistochemistry revealed a complete overlap between insulin and Aqp7 immunostaining in the pancreatic islet. Inactivation of Aqp7 by gene targeting produced viable and healthy mice. Aqp7 an increased intraislet glycerol a concomitant of the glycerol kinase level enzyme activity. The islet triglyceride content in the Aqp7 (cid:2) / (cid:2) increased compared to in the Aqp7 (cid:3) / (cid:3) mice. Aqp7 (cid:2) / (cid:2) mice reduced (cid:1) -cell mass and insulin content but increased insulin-1 and insulin-2 mRNAs. The reduction of (cid:1) -cell mass in Aqp7 (cid:2) / (cid:2) mice can be at least in part by a reduction in cell proliferation through protein kinase C and the c- myc cascade, with a reduction in the transcript levels of these two genes. Concomitantly, there was a decreased rate of apoptosis, as reﬂected by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling and caspase 3 and Bax expression in Aqp7 (cid:2) / mice. Compared with Aqp7 (cid:3) / (cid:3) islets, islets isolated from Aqp7 (cid:2) / secreted insulin at a higher rate under basal low-glucose conditions and on exposure to a high (450 mg/dl) glucose concentration. Aqp7 (cid:2) / (cid:2) exhibited normal fasting blood glucose levels but elevated blood insulin levels. Their plasma glucose response to an intraperitoneal (i.p.) glucose tolerance test was normal, but their plasma insulin concentrations were higher than those of wild-type mice during the 2-h test. An i.p. insulin tolerance test showed similar plasma glucose lowering in Aqp7 (cid:2) / (cid:2) and Aqp7 (cid:3) / (cid:3) mice, with no evidence of insulin resistance. In conclusion, we found that pancreatic (cid:1) cells AQP7, which appears to be a key of
A New Member of the Protein Kinase C Family, nPKCO, Predominantly Expressed in Skeletal Muscle

A new protein kinase C (PKC)-related cDNA with unique tissue distribution has been isolated and characterized. This cDNA encodes a protein, nPKCO, which consists of 707 amino acid residues and showed the highest sequence similarity to nPKC8 (67.0% in total). nPKCO has a zinc-finger-like cysteine-rich sequence (Cl region) and a protein kinase domain sequence (C3 region), both of which are common in all PKC family members. However, nPKCO lacks a putative Ca2+ binding region (C2 region) that is seen only in the conventional PKC subfamily (cPKCa, -13I, -1311, and -y) but not in the novel PKC subfamily (nPKC8, -e, -C, and -i). Northern (RNA) blot analyses revealed that the mRNA for nPKCO is expressed predominantly in skeletal muscle. Furthermore, nPKCO mRNA is the most abundantly expressed PKC isoform in skeletal muscle among the nine PKC family members. nPKCO expressed in COS1 cells serves as a phorbol ester receptor. By the use of an antipeptide antibody specific to the D2-D3 region of the nPKCO sequence, nPKCO was recognized as a 79-kDa protein upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis in mouse skeletal muscle extract and also in an extract from COS1 cells transfected with an nPKCO cDNA expression plasmid. Autophosphorylation of immunoprecipitated nPKCO was observed; it was enhanced by phosphatidylserine and 12-O-tetradecanoylphorbol-13-acetate but attenuated by the addition of Ca2+. These results clearly demonstrate that nPKCO should be considered a member of the PKC family of proteins that play crucial roles in the signal transduction pathway.
Relative functional load determines co-articulatory movements of the tongue tip*

Does morphological structure affect articulation when segmental similarity is strictly controlled? To address this question, we used electromagnetic articulography to study the articulatory trajectories of tongue tip and tongue body during the articulation of German words containing [ a(:) ] as stem vowels followed by [ t ] that in roughly half of the words realized an inﬂectional function. According to a generalized additive model ﬁtted to the articulatory trajectories of tongue body and tongue tip sensors using electromagnetic articulography, a factorial predictor signaling the presence or absence of an inﬂectional exponent underperformed compared to a quantitative measure derived from a Linear Discriminative Learning model. This quantitative measure is based on the functional load of triphones, i.e., the extent to which a triphone contributes to the meaning of the word. The relative functional load of the stem triphone (centered around [ a(:) ]) and the triphone pivoted on the [ t ] emerged as a strong co-determinant of articulation. Importantly, words with a balanced relative functional load (i.e., a value close to zero) revealed optimized smooth co-articulation at tongue body and tongue tip sensors. These results provide evidence for the possibility that differences in the details of articulation straight-forwardly reﬂect differences in meaning as captured by distributional semantics.
A Micro-Biomanipulation Training System based on Mixed-Reality

— Within neuroscience, micromanipulation and microinjection of cells (blastocysts and neurons) are essential and highly skilled tasks that can require years of training. These tasks are traditionally performed via direct manual control of the biomanipulation equipment while looking through a microscope. Yet, even after extensive training, yield can be low (40 – 70%). This paper presents a mixed-reality system for the training of operators (biologists/neuroscientists) on a new fully teleoperated biomanipulation system with reduced training requirements and much higher yields. Two mixed-reality training scenarios were designed, implemented and tested for this purpose: A “move-and-inject” task focused on precise positioning training; and a trajectory following scenario intended to develop precise motion control skills in new operators. Preliminary experiments performed with 20 totally novice operators demonstrate that this new training system is effective in terms of the initial development of control skills for real teleoperated biomanipulations. Experimental metrics demonstrate an exponential learning curve for these novice operators, who achieve good performance values after only two practice runs on the system. In addition, this training is shown to be safe and inexpensive since no real cells, biochemical products, or several pipettes are needed for this initial training phase.