Miracle-dz/UniFAR-FTUs huggingface dataset:
A map-based approach to assessing genetic diversity, structure, and connectivity in the seagrass Halodule wrightii

: Seagrass cover has declined in many areas of the world in a trend that has acceler-ated over the past several decades. This raises concern for both the impact the decline in cover has on coastal ecosystems and the effect it may have on seagrass evolutionary potential, as genotypic and genomic variation is lost. We used 8 microsatellite loci to investigate genetic diversity, structure, and connectivity in the seagrass Halodule wrightii from the Gulf of Mexico (Texas, USA) and western Atlantic (Bermuda). We examined how estimates correlated with changes in H. wrightii abundance and distribution on the Texas Gulf coast over the past 50 yr. Results show that, compared to other species, H. wrightii from this region exhibits variable clonal diversity ( R = 0.02−0.81), moderate allelic diversity (mean A R = 4.09), and relatively high heterozygosity (mean H e = 0.56). The patterns of genetic diversity and structure, however, do not entirely coincide with either geography or recent historical trends in seagrass distribution in this region. Results from a basin in which seagrasses have recently been expanding were consistent with expectations, as they were for an isolated site near the limit of H. wrightii ’s range. Results from basins in which seagrasses have been experiencing decline and/or fragmentation, however, were mixed. Genetic structure on the Texas coast was relatively weak and coincided more strongly with tidal range than with geographic barriers or distance. Rapid expansion and the discovery of identical multilocus genotypes at several sites raises the possibility of migration via drifting vegetative fragments, as the geographic distance among certain multi-locus genotypes cannot be explained by rhizome growth models.
Correlates and Mediators of Dissociation: Towards a Transtheoretical Perspective

We evaluated variables important to understanding dissociation (N = 379 undergraduates). We investigated: (a) the correlations among dissociation and impulsivity, alexithymia, mindfulness, negative affect, neuroticism, sleep disturbances, and emotion dysregulation; (b) unique variance of these variables in statistically predicting dissociation scores; and (c) the statistical mediational role of emotion dysregulation and sleep in explaining dissociation. We found significant positive correlations between dissociation and emotion dysregulation, sleep, alexithymia, negative affect, impulsivity, and neuroticism as well as a significant negative correlation between mindfulness and dissociation, consistent with Lynn et al. Sleep, impulsivity, emotion dysregulation, and negative affect uniquely related to and explained significant variance in dissociation, in order from most to least variance accounted for. Sleep partially mediated the relation between emotion dysregulation and dissociation and the relation between impulsivity and dissociation. Emotion dysregulation partially mediated the relation between sleep and dissociation and the relation between impulsivity and dissociation. Additional findings provided support for bidirectional relations between sleep experiences and dissociation and emotion dysregulation and dissociation.
Slaughter and carcass quality characteristics of Chios × Tahirova crossbred lambs under intensive, traditional and organic production systems.

Calismada, Afyon ilindeki ozel bir isletmede entansif, geleneksel ve organik uretim sistemlerinde yetistirilen Sakiz × Tahirova melezi kuzularin kesim ve karkas kalitesi ozelliklerinin ve cesitli karkas olculerinin belirlenmesi amaclanmistir. Entansif kuzular 71 gunluk yasta sutten kesilmisler ve kesime kadar agil icerisinde besiye alinmislardir. Geleneksel yetistirilen kuzular 108 gunluk yasa kadar agil icerisinde analari ile birlikte tutulmuslar, 108 gunluk yastan sonra ise analari ile meraya cikarilmislardir. 106 gunluk yasa kadar analari ile beraber agil icerisinde barindirilan organik kuzular, bu yastan sonra gunduzleri analari ile birlikte organik meraya cikarilmislardir. Organik kuzulara ayrica aksamlari organik olarak uretilmis kuru ot, arpa ve aycicegi kuspesinden olusan yem karmasi verilmistir. 30 kg canli agirliga ulasan kuzular kesime sevk edilmislerdir. Calismada geleneksel, entansif ve organik kuzularin ortalama besi sureleri sirasiyla 72, 79 ve 92 gun olarak tespit edilmistir (P<0.05). Farkli uretim sistemlerinde yer alan kuzularin karkas agirligi, karkas randimani ve karkas parcalarinin oranlari bakimindan benzer ortalama degerlere sahip olduklari belirlenmistir. Uzun buttaki et orani bakimindan geleneksel uretim sisteminde yetistirilen kuzularin diger uretim sistemlerine kiyasla daha yuksek ortalamaya sahip oldugu tespit edilmistir (P<0.05). Entansif kuzular ile kiyaslandiginda organik kuzularin omental ve mezenterik yag ve uzun butta derialti yag orani bakimindan daha dusuk ortalamalara sahip olduklari belirlenmistir (P<0.05). Sonuc olarak, karkas kalitesi parametreleri bakimindan entansif ve organik uretim sistemleri ile kiyaslandiginda geleneksel uretim sisteminin daha iyi sonuclar verdigi gorulmustur
Interaction of serum albumins with fluorescent ligand 4-azido coumarin: spectroscopic analysis and molecular docking studies

Steady state fluorescence and time resolved fluorescence studies at 298 K and low temperature phosphorescence (LTP) studies at 77 K of the interaction of bovine serum albumin (BSA) and human serum albumin (HSA) with ligand 4-azido-2H-chromen-2-one or 4-azidocoumarin (4-AC) have been carried out to visualize the location of the binding site and perturbation of the binding site of the tryptophan (Trp)/tyrosine (Tyr) of the protein(s) by monitoring the emission maxima of Trp residue(s) in proteins. The fluorescence quenching study of Trp estimated that the binding constant for both protein–ligand complexes is in the order of ∼106 with binding site 1. Perturbation in the secondary structures of serum albumins due to binding of 4-AC is also observed from circular dichroism (CD) studies. An energy transfer (ET) study further demonstrated that the non-radiative singlet–singlet ET that takes place from the Trp singlet states of proteins to the singlet state of ligands is greater in the case of BSA. This is supported by the distance and orientation of the donor–acceptor pair obtained from molecular docking studies. The molecular docking studies were also fruitfully exploited to understand the involvement of Trp213 in BSA and Trp214 in HSA in the ET process along with the perturbation of the residues around 5 A from the ligand 4-AC. Phosphorescence spectra at 77 K of the Trp residues in the free proteins (BSA/HSA) and in the complexes of BSA/HSA have also been utilized to specify the role of Trp residues in ET and the binding process.
Generating in vivo somatic mouse mosaics with locus-specific, stably-integrated transgenic elements

Viral vectors and electroporation (EP)-mediated gene transfers are efficient means of inducing somatic mosaicism in mice, but they lack the exquisite control over transgene copy number, gene zygosity, and genomic-locus specificity that genetically engineered mouse models (GEMMs) provide. Here, we develop and demonstrate a simple and generalizable in vivo method, mosaic analysis by dual recombinase-mediated cassette exchange (MADR). MADR allows for stable labeling of mutant cells express transgenic elements from a precisely-defined chromosomal locus. To test our method, we generated reporter-labeled lineages from stem and progenitor cells in a well-defined Rosa26mTmG mouse. We demonstrate the power and versatility of MADR by creating novel glioma models with mixed, reporter-defined zygosity or with “personalized” driver mutations from pediatric glioma—each manipulation altering the profile of resulting tumors. Thus, MADR provides a high-throughput genetic platform for the dissection of development and disease, and this rapid method can be applied to the thousands of existing gene-trap mice.
Turn structure and inserts

Abstract Turns-at-talk often do not start with their main business but rather with a pre-start (Sacks et al., 1974). This paper investigates the correlation of pre-starts with inserts, one of three major word classes (Biber et al., 1999). Based on the BNC’s mark-up, I investigate how inserts are positionally distributed in large amounts of turns of varied lengths. The analysis shows that inserts are overwhelmingly attracted to turn-first positions, the likely location of pre-starts. Further, in a subsample of 1,000 ten-word turns manually coded for pre-starts, 86% of all inserts serve a pre-start function. The findings call into question current speech processing models that fail to factor in turn structure. Further, pre-starts have crucial sequential and interactional implications as early indicators whether the new turn “agrees” with the prior turn and are likely key signals aiding listeners’ action ascription.
Cytotoxic Effects of Sábila (Aloe Vera): Commercial Products as Food Supplement

Aloe vera is used intensively in traditional medicine because it's widely number of therapeutic properties, and due to that it is used as food supplement. Although it is well known that the main class of compounds responsible for aloe-induced toxicity is anthraquinones, it seems to be that their concentrations are reduced in commercial products. To our knowledge, few studies concerning to the toxicity of aloe-based product have been documented. This lack of data suggested us to evaluate the effect of two different commercial products on cell viability, which were 200x Dehydrated Inner Leaf Powder (ILP) and 100x Dehydrated Whole Leaf Powder (WLP). The HPLC analysis revealed two peaks in each product; aloin (5.82 ppm for ILP and 4.75 ppm for WLP) and aloe-emodin (5.73 ppm for ILP and 1.68 ppm for WLP) indicating low concentrations compared with other studies. For cytotoxic analysis, both products were dissolved in cell culture medium getting final concentrations of 0.5x, 1x, 5x, and 10x; at pH 7.3 and 50 µg/ml gentamicine. Cells were analyzed by cell membrane integrity (LDH-release), metabolic activity (WST-1), functional lysosomes (NRU) and apoptosis induction (Annexin V/PI). The results indicated that both products are not cytotoxic at the recommended concentration of 1x, which it is used as food supplement; this could be explained due to the low anthraquinones concentration. The cytotoxic effects were observed from 5 up to 10x fold over-concentrated; a possible reason is that the toxic effect might be caused by mechanical stress by the fibres. For another side, no apoptotic effect was detected at concentrations up to 10x with both
How Can TV Food Programs Be Used as an Effective Restaurant Marketing Tool? An Extension of ELM with Perceived Risk

In today’s modern society, television (TV) food programs exert significant influence in the domestic and international foodservice market. This study applied the elaboration likelihood model (ELM) to examine the information processing and decision-making activities of restaurant customers related to TV food shows that they watched. The ELM was expanded and tested by including perceived risk as a factor influencing attitude towards a restaurant. A total of 347 responses obtained from Korean residents, who had obtained information delivered by a messenger in a TV program and had subsequently visited that restaurant in the three months prior to the survey were analyzed. Results revealed that the information quality components significantly influenced attitude towards restaurants. For the peripheral route, while the credibility of the messenger had no significant impact, the vividness of expression had a positive impact on attitude towards restaurants. Performance risk was shown to have a negative impact on attitude towards the restaurant. However, time risk, possibly requiring long-distance travel or long waiting times for available tables played a positive role. The long wait times may be perceived well because many people wanting to eat at the restaurants is a positive sign about the experience. Attitude towards a restaurant positively influenced visit intention. Based on the study’s findings, discussion, implications, and limitations for future studies are provided.
Impact of tyrosine phosphorylation of Syntaxin 4 and Munc18c on GLUT4 translocation

Insulin is an important regulator of glucose homeostasis. Insulin stimulation of fat and muscle cells results in the rapid translocation of the glucose transporter GLUT4 to the plasma membrane from its intracellular stores, allowing uptake of glucose into the cells from the blood. This tethering, docking and fusion event is driven by the formation of a SNARE complex at the plasma membrane consisting of the t-SNAREs Syntaxin4 (Sx4) and SNAP23 and the v-SNARE VAMP2. The formation of this complex is regulated by the SM protein Munc18c. It has been shown that Sx4 is phosphorylated at residues Y115 and Y251 following insulin stimulation; however the effects of these phosphorylation events have yet to be studied. In addition phosphorylation of Y521 in Munc18c is also increased following insulin stimulation. This study aimed to first elucidate the effects of Sx4 phosphorylation on SNARE protein interactions and GLUT4 trafficking, then to begin to examine the impact of phosphorylation of both Sx4 and Munc18c. I have used an in vitro approach to assess the affect Sx4 phosphorylation has on SNARE complex assembly. I have shown, using phospho-mimetic recombinant proteins, that the phosphorylation state of Sx4 affects the rate of SNARE complex assembly and its binary interactions with VAMP2 and SNAP23. Moreover, that this may be due to a conformational change in the protein. I have also shown, for the first time, that it is likely that Sx4 can be phosphorylated on Y115 and Y251 simultaneously. In addition I have used a HA-GLUT4-GFP expressing HeLa cell line to show that expression of phospho- mimetic Sx4 increases translocation of GLUT4 to the PM under basal conditions. Finally, I have begun to investigate the implications of both Sx4 and Munc18c phosphorylation on their in vitro interactions. This data provides insights into the direct regulation of membrane trafficking proteins by the insulin-signalling pathway. Increased understanding of the regulation of GLUT4 translocation could help to develop future therapies for type 2 diabetes, where GLUT4 trafficking is impaired.
Tracking and Influencing Learning Motivation

This paper reports on the incorporation of a motivational tracking system and regular interventions designed to address issues in motivation in an intensive English program at a Japanese university. Characteristics of the system are described and data are discussed. Ten intervention sessions focused participants’ (N = 40) attention on aspects of motivation, including goal-setting, habit formation, and imagery. The results indicate that motivational levels rose steadily during the term and suggest that motivation is not a static trait; rather, it is a state which can be influenced through appropriate interventions. The application of motivational interventions is discussed. 本稿は、日本の男女共学大学の英語インテンシブ・プログラムにおける英語学習動機の追跡システムと定期的計画的な学習動機を高めることを狙った教育的介入についての報告である。 学習動機を高める教育的介入システムの特徴とその結果について、収集したデータに基づいて分析を行った。 その結果、2クラスにおける参加者（N=40)の学習動機レベルは当該学期中継続的な伸びを示した。 10回の動機付け介入セッションは目標設定、習慣形成、イメージの利用など、様々な動機付けの側面に学習者の注意を集中させるように組み立てた。 本結果から、学習動機は、固定的な特性ではなく学習期間を通して適切な教育的介入を行なうことで影響を与えることができる、変化しうる心的状態であることが示唆された。 英語学習プログラムを効果的なものにする為の、学習動機を高める様々な教育的介入についての議論を行った。
Selective mode excitation of Lamb wave in composite laminates for a circular and two ring‐shaped piezoelectric actuators

In the current research the effect of the radius of a transducer, vibration frequency and anisotropic properties of the host structure on the occuring wave fields is analyzed. Semi‐analytical approach based on the Green's matrix representations and the Fourier transform is applied for calculation of dispersion curves and wave fields in two types of composite layups in a wide frequency‐thickness product range. The contribution of each wave mode in the excited wave field is analyzed. A method for decomposition of the Lamb waves is proposed and examined for the example of a combination of two ring‐shaped actuators.
Two-dimensional characterization of three-dimensional nanostructures of magnetic bubbles in Fe3Sn2

We report differential phase contrast scanning transmission electron microscopy (TEM) of nanoscale magnetic objects in Kagome ferromagnet Fe 3 Sn 2 nanostructures. This technique can directly detect the deflection angle of a focused electron beam, thus allowing clear identification of the real magnetic structures of two magnetic objects including three-ring and complex arch-shaped vortices in Fe 3 Sn 2 by Lorentz transmission electron microscopy imaging. Numerical calculations based on real material-specific parameters well reproduced the experimental results, showing that the magnetic objects can be attributed to integral magnetizations of two types of complex three-dimensional (3D) magnetic bubbles with depth-modulated spin twisting. Magnetic configurations obtained using the high-resolution TEM are generally considered as two-dimensional (2D) magnetic objects previously. Our results imply the importance of the integral magnetizations of underestimated 3D magnetic structures in 2D TEM magnetic characterizations.
Effect of spectrogram resolution on deep-neural-network-based speech enhancement

: In recent single-channel speech enhancement, deep neural network (DNN) has played a quite important role for achieving high performance. One standard use of DNN is to construct a mask-generating function for time-frequency (T-F) masking. For applying a mask in T-F domain, the short-time Fourier transform (STFT) is usually utilized because of its well-understood and invertible nature. While the mask-generating regression function has been studied for a long time, there is less research on T-F transform from the viewpoint of speech enhancement. Since the performance of speech enhancement depends on both the T-F mask estimator and T-F transform, investigating T-F transform should be beneﬁcial for designing a better enhancement system. In this paper, as a step toward optimal T-F transform in terms of speech enhancement, we experimentally investigated the eﬀect of parameter settings of STFT on a DNN-based mask estimator. We conducted the experiments using three types of DNN architectures with three types of loss functions, and the results suggested that U-Net is robust to the parameter setting while that is not the case for fully connected and BLSTM networks.
First occurrence of Ibacinae (Eucrustacea: Decapoda: Scyllaridae) from the Eocene of Pakistan

The fossil record of slipper lobsters (Scyllaridae) is very poor, even though the earliest species of the modern slipper lobsters (Neoscyllaridae) are known from the Early Cretaceous. Among the clades (‘subfamilies’) of slipper lobsters, perhaps the most remarkable is Ibacinae (currently monogeneric), which contains species with a very wide carapace (cephalothoracic shield) and very deep incisions. Fossil species assigned to Ibacinae were reported from the Oligocene and Pleistocene. However, a recent reappraisal of Scyllaridae phylogeny revealed that the genus to which these two fossil occurrences belong is not closely allied to Ibacinae. Therefore, Ibacinae was left with no known fossil record until the present study. The present study describes a new species, which can be confidently assigned to Ibacinae (closely allied to Ibacus), from the Ypresian (early Eocene) of the Ghazij Formation in Shaisuro, Dera Ghazi Khan District, Pakistan. This new species, Ibacus? mazariorum sp. nov., corresponds to the oldest recorded occurrence of Ibacinae. The Ghazij Formation was deposited on the north-western margin of the India-Pakistan plate during a critical period, shortly before the collision between the India-Pakistan plate and Asia. Consequently, it is possible that Ibacus and closely allied species may have remained in the same biogeographical area since the Eocene. Considering the extant and fossil distribution of Ibacinae, additional fossil discoveries may be found in existing collections and fossiliferous outcrops from the Neotethys, Indian and Pacific oceans. http://zoobank.org/urn:lsid:zoobank.org:pub:84F54514-AA21-4A9D-91CA-BDCACBA5F4F8
On the relationship between psychopathy and general intelligence: A meta-analytic review

Over recent decades, a growing body of research has accumulated concerning the relationship between indicators of general intelligence and the personality construct known as psychopathy. Both traits represent key correlates of life outcomes, predicting everything from occupational and economic success, to various indicators of prosocial behavior (including avoiding contact with the criminal justice system). The findings to date regarding the association of the two traits, however, have been somewhat inconsistent. Thus, there remains a need for a more systematic investigation of the extant empirical literature. The current study reports a meta-analysis conducted to evaluate the direction and overall effect size of the relationship between these two constructs. Our analyses revealed a small, but significant, negative effect of intelligence on psychopathy. The results and impact of possible moderating variables such as type of intelligence test used are discussed. Finally, the study limitations, and possible directions for further research on this issue are detailed prior to concluding.
Impact of different additives on spawn production of different strains of lion’s mane mushroom (Hericium Spp.)

Medicinal mushrooms have bioactive compounds with therapeutic properties. Lion’s Mane Mushroom is found throughout the northern hemisphere in Europe, Asia, and North America. The present investigation was conducted with the aim to determine the effect of organic and inorganic additiveon mycelium growth (spawn) of Lion’s mane mushroom (Hericium Spp.). In the present study organic additive viz. pulses flour (Pigeon pea) and cereal flour (Rice flour) @2% and inorganic additive (MgSo4) were mixed @1% as a supplement with wheat grains for spawn production of six different strains of Hericium. The results obtained during the present investigation, in the spawn production maximum mycelial growth (90.00 mm) was found in strain He-02 in the organic additive Pigeon pea flour and also in cereal flour (Rice flour) on 20th days, while maximum mycelial growth (90.00 mm) was also found in strain He-02 when Mgso4 was mix in wheat grains for spawn production of six strains of Hericium as inorganic supplements. Based on the results obtained, for spawn production of Lion’s mane mushroom (Hericium Spp.) strains He-02, by organic and inorganic supplementation, Pigeon pea flour and rice flour while in case of inorganic additive Mgso4 would be recommended most effective supplement in wheat grains for strain He-02.