Miracle-dz/UniFAR-FTUs huggingface dataset:
SAT0059 Inhibition of CIP2A facilitate C-MYC independent apoptosis of fibroblast-like synoviocytes in rheumatoid arthritis

Background Cancerous inhibitor of protein phosphatase 2A (CIP2A) is a recently identified oncoprotein that leads to cellular proliferation in cancer cells by stabilizing c-Myc protein. The effect of CIP2A in stabilizing c-Myc by inhibition of protein phosphatase 2A activity is a prerequisite step in tumor cell growth and in vivo tumor formation. We have previously shown that CIP2A is expressed in RA FLS and its expression is strongly associated with histopathological synovitis score and invasive function of RA FLS. However, the effect of CIP2A in association with c-Myc on the apoptosis of RA FLS is unknown. Objectives This study was undertaken to investigate the effect of CIP2A and c-myc on the apoptosis of RA FLS and to determine the signaling pathway through which dysfunctional apoptosis is facilitated. Methods Proliferation and apoptotic activity of RA FLS following treatment with CIP2A siRNA or control siRNA were analyzed using MTT assays and Cell Death Detection ELISA kit. c-Myc expression from RA FLS after treatment with CIP2A siRNA or control at 3, 6, 9 days interval was also determined using Western blot analysis. To evaluate which signal transduction pathways were engaged in apoptosis, caspase-3 activity and phosphorylation of the Akt kinase were also analyzed by Western blot. Results Survival of RA FLS as measured by MTT assay was significantly lower in CIP2A siRNA -treated group compared to control after 7 days (n=5, each, p=0.0221). Apoptosis of RA FLS as measured by DNA fragmentation was significantly higher in CIP2A siRNA treated group compared to control when incubated for 3, 6 and 9 days (p=0.029, p=0.021, p=0.043, respectively). C-Myc expression measured by Western blot did not change during the incubation period. Apoptosis of CIP2A siRNA-treated RA FLS was induced by activation of caspase-3 and dephosphorylation of Akt kinase, but these findings were not observed in the control siRNA-treated RA FLS. Conclusions Our data show that CIP2A expression in RA FLS is an important mediator of dysfunctional apoptosis that is independent of c-Myc stabilization. CIP2A may induce dysfunctional apoptosis of RA FLS by activation of Akt and deactivation of caspase-3. These results warrant further investigation of CIP2A as a potential pathogenetic factor and therapeutic target in RA. Disclosure of Interest None Declared
Structure and electrical properties of Eu-doped SrBi2Nb2O9 ceramics

Structure and dielectric properties of Eu-doped SrBi2Nb2O9 ceramics (with 0, 20 and 35 at.% of Eu), prepared by the solid-state method and sintering, were investigated. XRD, FTIR and SEM measurements were provided to validate the characteristic structural features of the obtained ceramics. For all samples, the orthorhombic structure was identified through XRD analysis. SEM results confirmed that the fabricated samples have relatively dense structure with rod- and plate-like grains typical for Aurivillius layered structures. Dielectric results showed that the doping with Eu decreases dielectric constant and reduces dielectric loss. Movement of the dielectric peak towards higher temperatures appearing at about 400? C with increase of frequency indicates on relaxor behaviour of the sample with 35 at. % of Eu.
Risk Factors in Coronary Heart Disease An Evaluation of Several Serum Lipids as Predictors of Coronary Heart Disease

AT FRAMINGHAM, MASSACHUSETTS, the Na±\. tional Heart Institute has been follow­ ing 5,127 men and women aged 30 to 60 years in a study of factors related to the development of coronary heart disease (CHD) and hypertension. Data for a dec­ ade of follow-up studies of this population are available for analysis. From a study of those who have de­ veloped disease under observation in com­ parison to those who have remained free of the disease, factors of risk and suscep­ tible individuals have been identified. The factors associated with excess risk that have been identified are: serum cholesterol level, blood pressure, cigarette smoking, vital capacity, relative weight, and certain elec­ trocardiographic findings. Efforts are being made to assess the independent contribu­ tion of each of these factors to the develop­ ment of coronary heart disease because of the therapeutic, preventive, and patho­ genetic implications of this information. This report focuses on the serum lipids. Attempt will be made to assess the relative efficacy of the various lipids as predictors of coronary heart disease and to determine their independent contribution to risk of the development of the disease. The present report deals with three types of lipids: cholesterol, phospholipids, and various beta-lipoprotein fractions. The relationship of various indexes, based on these lipids, to development of disease will also be assessed. Evaluation of the serum trigly­ cerides in relation to development of dis­ ease will not be included, since this meas­ urement was introduced in the study too recently for prospective analysis. METHODS
High-resolution EBSD characterisation of friction stir welded nickel–copper alloy: effect of the initial microstructure on microstructural evolution and mechanical properties

ABSTRACT The effect of the initial structure on the microstructure and texture of the friction stir welded Monel 400 alloy joints was investigated. For this aim, Monel 400 alloy of two initially treated conditions, annealed and rolled, was studied. Orientation imaging microscopy with a step size of 70 nm was used to characterise the microstructures of the joints. The mechanical properties were evaluated using Vickers hardness and nanoindentation tests. In the joint of the initial annealed alloy, continuous dynamic recrystallisation governed the grain structure formation. However, in the case of the initial rolled base material, discontinuous dynamic recrystallisation also occurred to cause more random texture, smaller grains, much more high-angle grain boundaries and higher dislocation densities. In the stir zone, the hardness and yielding strength increased respectively from 177 HV to 192 HV and 215.9 to 238.8 MPa by changing the base metal from the initial annealed to rolled condition. In addition, the microstructural features of the different zones containing the texture components and grain boundaries were scrutinised thoroughly.
Modeling and Observer-Based Monitoring of RAFT Homopolymerization Reactions

Reversible addition–fragmentation chain–transfer (RAFT) polymerization of methyl methacrylate (MMA) is modeled and monitored using a multi-rate multi-delay observer in this work. First, to fit the RAFT reaction rate coefficients and the initiator efficiency in the model, in situ 1 H nuclear magnetic resonance (NMR) experimental data from small-scale (<2 mL) NMR tube reactions is obtained and a least squares optimization is performed. 1 H NMR and size exclusion chromatography (SEC) experimental data from large-scale (>400 mL) reflux reactions is then used to validate the fitted model. The fitted model accurately predicts the polymer properties of the large-scale reactions with slight discordance at late reaction times. Based on the fitted model, a multi-rate multi-delay observer coupled with an inter-sample predictor and dead time compensator is designed, to account for the asynchronous multi-rate measurements with non-constant delays. The multi-rate multi-delay observer shows perfect convergence after a few sampling times when tested against the fitted model, and is in fair agreement with the real data at late reaction times when implemented based on the experimental measurements.
Development of an adolescent alcohol misuse intervention based on the Prototype Willingness Model: A Delphi study

Purpose – The purpose of this paper is to report on the use of the Delphi method to gain expert feedback on the identification of behaviour change techniques (BCTs) and development of a novel intervention to reduce adolescent alcohol misuse, based on the Prototype Willingness Model (PWM) of health risk behaviour. Design/methodology/approach – Four BCTs based on the PWM were identified and incorporated into a draft intervention that aimed to change alcohol prototypes and enable adolescents to deal with social pressure. Using the Delphi process, successive questionnaires were distributed to 20 international experts to build consensus on the theoretical validity of the intervention. Findings – In total, 15 experts completed round 1 and 11 completed round 2 of the Delphi study. A high level of consensus was achieved. Four priority areas were identified to improve the intervention: incorporating extra techniques to address social pressure; increasing intensity; providing incentives; and addressing credibility.
Entropy based Registration of Transmission Electron Microscopy Image Stacks

Analytic transmission electron microscopy provides a set of well-established imaging methods for chemical and structural material analysis on a nano-base. A series of distortions make the resulting image stacks suffer from inconsistencies in illumination, nonisochromaticity, noise and especially spatial drift. These preconditions exacerbate material analysis and require a robust, general and highly accurate alignment procedure which is realized by a combined approach of proper preprocessing, entropy-based similarity measures and structural information. In this master’s thesis, entropy-based similarity measures and their limitations are investigated in detail, since they influence the registration result tremendously. Different bundle adjustment techniques are applied in a post processing step and are evaluated to gain a globally optimal alignment result. Finally, a comprehensive comparison of the presented methods is given on synthetic and real data sets.
Micro-structural characterization of glassy solids

In physics, the term ‘solid’ is frequently associated with crystals, despite the fact that there is a vast number of solid structures that are not crystalline, such as amorphous or glassy solids. Due to their periodic structures and long-range order, even complex crystalline structures can be studied with the help of mathematical tools such as Bloch states, Brillouin zones, k-space, etc. Glassy solids, on the other hand, do not offer any mitigating circumstances with respect to their theoretical treatment due to their lack of long-range order and low predictability of internal structure. Nevertheless, glassy materials can be studied experimentally, theoretically, and by using computer simulations. In this thesis, we firstly study the properties of glasses close to the yielding transition. The yielding transition occurs as the amorphous solid is irreversibly deformed beyond its elastic limit. Though extensively studied, this transition is still not well understood. We take a look at this elusive phenomenon at a micro-mechanical level and develop an approach that has embedded within it the very carriers of plasticity that are responsible for it. Secondly, we explore the free energy in the hard sphere model and its experimental equivalent-colloidal glass. Specifically, we aim to establish local-particle level-free energy as a bridge between micro-structure and glassy dynamics.
Quantification of the timing of anterior fontanelle closure in a Western Australian population

Closure of the anterior fontanelle is generally accepted to occur within the first two years of life. However, statistical quantification of the precise timing of closure in a Western Australian population has not been explored. The current study examined MDCT scans of 68 individuals between birth and 3.91 years to assess the timing of anterior fontanelle closure. Using OsiriX, the scans are viewed in axial and multi-planar reconstructed (MPR) images and three-dimensional volume rendered reconstructions. Anterior fontanelle fusion status was quantified using two methods: calculating anterior fontanelle area and by taking the oblique linear distances of the open fontanelle. The single greatest proportion of fusion was shown to occur in the first year of life, by which stage only 47.19% of the fontanelle remains unfused (on average by area). There was only one individual demonstrating complete fusion before 1 year of age (0.86 years) with no single individual presenting an open fontanelle beyond 2.4 years of age. The current study outlines statistically quantitated data that facilitates forensic age estimation (either macroscopic or radiographic) specific to Australian individuals from the perinate to early childhood life stages.
Effect of Cervical Corrective Exercises on Pain, Neck Posture, and Intersegmental Motion of Cervical Spine in a Patient With Cervical Radiculopathy: A Case Report

This case report describes the effectiveness of cervical corrective exercises in a patient with cervical radiculopathy (CR) who experienced radicular pain, upper limb paresis, and limited functional activity. A 39-year-old male with cervical radiculopathy performed the cervical corrective exercises for reducing pain. Pain intensity, cervical posture, and active range of motion of cervical intersegmental spine motion were measured baseline, after 4 weeks, and after 8 weeks with self-reported questionnaire and radiographs. After 8 weeks of intervention, the patient demonstrated alleviated radicular symptoms, improved neck posture and active range of flexion and extension of the cervical intersegmental spine. Especially in the angle between the cervical vertebra 6 and 7, the angle was changed from to during resting position after intervention. The present case indicates that the cervical corrective exercises might be a possible treatment to effectively reduce radicular symptoms, improve neck posture, and active cervical intersegmental motion for patient with CR.
A new method to evaluate interlacing yarns

The theory of purification is proposed in this article. Based on the framework of this theory, several models can be built to give a synthetic quality evaluation of interlacing yarns. In this paper, three models are given. The first one is the most popular method to evaluate the quality of interlacing yarn and we can prove it meets the theory of purification. The second one is modified according to the coefficient of variation. The third one is a new evaluation method. Meanwhile, we give a test algorithm to compare the degree of reflecting common cognition of the three models. From the result of comparison and analysis, the new evaluation method is recommended. It has a comparatively complete evaluation value for every state in universe M. Compared to other modeling methods, this method can give every level of interlacing yarn a relatively pertinent evaluation.
Effects of Organic and Inorganic Selenium Supplementation during Late Gestation and Early Lactation on Beef Cow-Calf Performance

Muegge, Chris R. , M.S., Purdue University, December 2015. Effects of Organic and Inorganic Selenium Supplementation during Late Gestation and Early Lactation on Beef Cow-Calf Performance. Major Professor: Jon Schoonmaker Angus x Simmental cows (n = 48, BW = 594 kg, BCS = 5.26, Age = 2.7), pregnant with male fetuses, were used to determine the effect selenium (Se) source during the last 80 d of gestation and first 108 d of lactation on progeny feedlot performance. At 203 d in gestation, cows were blocked by body weight, age, breed composition, and calf sire, and allotted to 1 of 3 treatments: no Se, inorganic Se, or organic Se. Maternal diets were formulated to contain 10.4% CP and 0.90 Mcal/kg NEg during gestation and 12.1% CP and 1.01 Mcal/kg NEg during lactation. Basal diets contained 0.07 and 0.11 mg/kg Se for gestation and lactation diets; respectively. Diets were fed daily as a total mixed ration and Se was provided in a top-dress containing 0, 0.30 mg/kg Se as sodium selenite, or 0.30 mg/kg Se as Sel-Plex®. Treatment diets were fed through 108 d postpartum (DPP). At 68 DPP milk production was calculated using the weigh-suckle-weigh procedure and a milk sample was collected was at 62 DPP to determine composition. At 108 DPP cow-calf pairs were commingled until weaning at 216 DPP. At 28 d postweaning, steers (n = 47, BW = 301 kg) were placed in individual pens and fed a diet formulated to provide 13.9% CP, 1.24 Mcal/kg NEg, and 0.10 mg/kg Se. The diet was
A phantom based study of short-wave infrared emitting nanocomposites as contrast agents for fluorescence guided surgery

OF THE THESIS . ii ACKNOWLEDGEMENTS . iv DEDICATION . vi TABLE OF CONTENTS . vii LIST OF TABLES . ix LIST OF FIGURES . x CHAPTER 1: INTRODUCTION . 1 1.1: Cancer and Standard Treatments . 1 1.2: Fluorescence-Guided Surgery . 2 1.2.1: Concepts in Fluorescence Imaging . 4 1.2.2: Commercial Surgical Guidance Systems and Contrast Agents . 8 1.3: Short-Wave Infrared Light . 12 1.4: Rare Earth Nanocomposites . 16 1.4. 1: Composition . 16 1.4. 2: Advantages. 18 1.5: Aims . 19 1.5. 1: Motivation . 19 1.5. 2: Overview . 19 1.6: Hypothesis . 20 CHAPTER 2: INSTRUMENT DESIGN . 21 2.1: Hardware . 21 2.1.1: SWIR Emission Filters . 21 2.1.2: Illumination of Rare Earth Phantoms . 27 2.1.3: Illumination of ReANC and ICG Phantoms . 30 2.1.4: Final Design . 32 2.2: Software . 34 CHAPTER 3: PHANTOM STUDY . 38 3.1: ReANC Synthesis . 38 3.2: Agarose-Gelatin Phantoms . 39 3.2.1: Rationale . 39 3.2.2: Fabrication . 40 3.3: Chicken Breast Experiments . 42 3.4: Inclusion Size and Depth Experiments . 46
Monte–Carlo simulation of wall correction factor of graphite cavity ionisation chamber

: The purpose of this article is to discuss the method of determining the wall correction factor of graphite cavity ionisation chamber. The method is the ‘equal effect wall thickness’ simulation and direct calculation of the wall correction factor of the std10cm 3 and std30cm 3 spherical graphite cavity ionisation chamber in the national air-specific kinetic energy reference group (National Institute of Standards and Technology NIST), respectively, in the NIST10cm 3 and NIST30cm 3 spherical graphite cavity ionisation chamber. The calculated results are in agreement with the values released by NIST in 0.1%. Conclusions of the equivalent wall thickness simulation method and the direct calculation simulation method meet each other's requirements, which provide a new idea for the determination of wall correction factor.
A3_4 ‘Afraid of a Little Lightning?’: Mjolnir’s Capacitance

Thor’s hammer, Mjolnir, appears to store the energy from lighting. By modelling Mjolnir as a parallel plate capacitor, with a hollow centre, we calculated that it has a capacitance of 2.1 × 10^ − 11 F . To store the energy of one lightning bolt, an electric field of 1.6 × 10^ 12 Vm − 1 is created. This would ionise any air inside Mjolnir, and so a dielectric is needed to maintain the capacitor.
MATGENE: A program to develop job-exposure matrices in the general population in France

Objectives Matgéné is a program to develop job-exposure matrices (JEM) adapted to the general population in France for the period since 1950. The aim is to create retrospective exposure assessment tools for estimating the prevalence of occupational exposure to various agents that can then be correlated to health-related parameters. Methods Each JEM is specific to one agent, assessing exposure for a set of homogeneous combinations (occupation x activity x period) according to two occupational classifications (ISCO 1968 or PCS 1994) and one economic activities classification (NAF 2000). The cells of the JEM carry an estimate of the probability and level of exposure. Level is estimated by the duration and intensity of exposure-linked tasks. Results By the end of 2010, 18 JEMs have been developed and 8 are under development, concerning a variety of agents: organic and mineral dust, mineral fibres and solvents. These JEMs were applied to a representative sample of the French population and prevalence (at different dates and for complete careers) for each exposure was estimated in various population groups; attributable risk fractions were also estimated for certain pathologies. Some of these results were validated by comparison with those of other programs. All JEMs are available on a dedicated website. Conclusions Initial Matgéné JEMs results are in agreement with the French and international literature, thus validating the methodology. These JEMs are important epidemiological tools to assess occupational exposure for large retrospective community-based epidemiological survey in France.
The 10-20 Electrode System and Cerebral Location

ABSTRACT.In the three decades since it was introduced, the International 10-20 System of electrode placement has become the standard for locating scalp electrodes in EEG. The technique employs measurements of external cranial landmarks to locate the electrodes on the scalp. This process assumes a consistent correlation between scalp electrode locations and underlying cerebral structures.
Nanofluidic peristaltic pumps made from silica thin films

We present theory for operation and experimental results for a nanoscale pump implemented in silica thin films that uses electrostatic actuation. The devices were implemented on silicon substrates using standard microfabrication recipes. Using pressures induced by capillary forces, the pressures exerted on pump membranes through electrostatic forces, and the approximate displacement per stroke we predict the pump speed operating the device over a range of frequencies and voltages. For membranes 100 nm thick, 170 V was required for pump actuation, providing exquisite control of pumping rates of less than 1 fl s−1 per nanochannel.
On exact wave propagation analysis of triclinic material using three-dimensional bi-Helmholtz gradient plate model

Rapid advances in the engineering applications can bring further areas to provide the opportunity to manipulate anisotropic structures for direct productivity in design of micro/nano-structures. For the first time, magnetic affected wave characteristics of nanosize plates made of anisotropic material is investigated via the three-dimensional bi-Helmholtz nonlocal strain gradient theory. Three small scale parameters are used to predict the size-dependent behavior of the nanoplates more accurately. After owing governing equations of wave motion, an analytical approach based harmonic series is utilized to fine the wave frequency as well as phase velocity. It is observed that the small scale parameters, magnetic field and wave number have considerable influence on the wave characteristics of anisotropic nanoplates. Due to the lack of any study on the mechanics of three-dimensional bi-Helmholtz gradient plates made of anisotropic materials, it is hoped that the present exact model may be used as a benchmark for future works of such nanostructures.