Miracle-dz/UniFAR-FTUs huggingface dataset:
Annexin 2 expression is reduced in human osteosarcoma metastases

Osteosarcoma is an aggressive primary bone cancer affecting primarily children and young adults. The development of valuable diagnostic indicators and therapeutic agents will be enhanced by the identification and characterization of genes that contribute to its aggressive behavior. We used representational difference analysis to isolate genes differentially expressed between primary human osteosarcoma tumors and subsequent metastatic lung lesions to identify genes potentially involved in metastatic potential. Several genes were differentially expressed between the two tumor populations, including annexin2. The levels of annexin2 mRNA and protein inversely correlated with metastatic potential in a subset of human osteosarcoma tumor specimens, as well as in a human osteosarcoma cell line selected for increased metastatic potential. Annexin2 has been described in several cellular localizations with various functional implications, many of which may be relevant to metastatic potential. Therefore, the subcellular localization of endogenous annexin2 protein was evaluated biochemically by subcellular fractionation and immunologically by flow cytometry and immunofluorescence in osteoblastic cells. Annexin2 was localized to the cytoplasm and intracellular aspect of the plasma membrane, excluded from the nucleus and undetectable on the cell surface or in the conditioned medium. Overexpression of annexin2 in osteosarcoma cells did not alter several in vitro phenotypes often used to assess metastatic potential including motility, adhesion, and proliferation. However, our previous data have implicated annexin2 in the mineralization process of osteoblastic cells in vitro. Consistent with an increase in differentiation‐induced mineralization, there was diminished tumorigenicity and experimental metastatic potential of osteosarcoma cells overexpressing annexin2. These data suggest that annexin2 may downregulate osteosarcoma aggressiveness by inducing a more differentiated state in osteoblastic cells. © 2004 Wiley‐Liss, Inc.
DATS: A Distributed Algorithm for Time Synchronization in Wireless Sensor Network

In this paper, for decrease transmissions delay in wireless sensor network, a distributed algorithm (DATS) suggested. Because this network have got limited energy, therefore the energy issue is very important. The exist protocols due to have transmission delay in sending data among nodes cause increase consuming energy in network. DATS because of is distributing solve the problem. In this algorithm, a distinguished node periodically sends time values along a spanning tree structure. One of the most important advantages of the DATS is purely distributed, local, and does not depend on a fixed structure for disseminating time values. In conclusion, results gained shows that suggested protocol decrease transmissions delay of wireless network above 30 percent compared to the exist approaches.
Early Infections in Renal Transplant Recipients: Incidence, Risk Factors, and Causative Microorganisms.

OBJECTIVES This study aimed to compare renal transplant recipients with and without infection with respect to demographic and clinical characteristics as well as risk factors; to determine the incidence of posttransplant infections; and to study the antibiotic resistance patterns of bacterial species identified as the causative organisms in posttransplant infections. MATERIALS AND METHODS This study included a total of 136 patients undergoing renal transplant in a 4-year period. The patients were categorized into 2 groups. The 2 groups were compared with each other with respect to general clinical and demographic variables and the number and frequency of infectious attacks within a 1-year follow-up, infection type, and antibiotic resistance patterns. RESULTS Ninety-two (67.6%) of the subjects were male and 44 (32.4%) were female. A total of 57 (41.9%) patients developed 128 infectious attacks. Urinary tract infections were the most common infections (42.1%). There was a significant correlation between a clinically relevant urinary culture proliferation and postoperative infection rate (P = .002). There was a significant correlation between antimicrobial resistance and the number of infectious attacks (P = .023). There was a significant correlation between the proliferation of Extended Spectrum Beta Lactamase-positive Enterobactericeae species and the number of infectious attacks (P = .000). CONCLUSIONS Presence of a clinically relevant proliferation in the preoperative urinary culture, which was identified as a risk factor for infection, increased the number of infectious attacks. Moreover, Extended Spectrum Beta Lactamase-positive Enterobactericeae species increased the number of infections. These 2 principle results should be taken into account in patient management.
A Case for PARAM Shavak: Ready-to-Use and Affordable Supercomputing Solution

High Performance Computing (HPC) Systems are usually large systems which require specialized infrastructure. For a variety of small time users, who need performance of the parallel computing for their applications, such systems are unaffordable and inaccessible for a number of reasons. Even to setup a small state-of-the-art HPC system, such users would require vast efforts and expertise to design system specifications and to identify and install system software, tools and user applications. Also, going through such process would consume time and can be expensive. Clearly, there is a requirement of a small and low-cost ready-to- use HPC system which can be straightway put to utilization by end-users. In this paper, we present a case of a small, affordable and personalized supercomputing solution named PARAM Shavak [8, 9] which offers ready-to-use supercomputing-in-a-box solution based on commercial off-the-shelf HPC hardware resources. This solution is aimed as a support tool for research, design and development — often related to the education or small time designers. The solution is so architected that it provides scalability and power efficiency. We also discuss the uniqueness of our solution compared to several related initiatives which have been around and show its efficacy.
A novel mathematical modeling approach to the electric dispatch problem: Case study using Differential Evolution algorithms

Nowadays, the population growth and economic development causes the need for electricity power to increase every year. An unit dispatch problem is defined as the attribution of operational values to each generation unit inside a power plant, given some criteria to be obeyed like the total power to be generated, operational bounds of these units etc. In this context, an optimal dispatch programming for hydroelectric units in energy plants provides a bigger production of electricity to be generated with a minimal water amount. This paper presents an optimization solution for hydroelectric generating system of a plant, using Differential Evolution algorithms. The novel mathematical model proposed and validation of the obtained algorithms will be performed with practical simulation experiments. Throughout the text, the equations and models for the system simulation will be fully described, and the experiments and results will be objectively analysed through statistical inference. Simulation results indicate savings of 6.5 million litres of water for each month of operation using the proposed solution.
The Costimulatory Molecule B7 is Expressed on Human Microglia in Culture and in Multiple Sclerosis Acute Lesions

B7 is a constimulatory molecule which is expressed on antigen-presenting cells and which plays a pivotal role in T cell activation and proliferation. To elucidate mechanisms regulating intracerebral immune responses, expression of B7 was examined in cultured microglial cells and in brain tissue from control and multiple sclerosis patients. Using immunocytochemical and polymerase chain reaction techniques, we show that B7 was expressed in cultured microglial cells from the human embryonic brain. Microglia also bound the soluble form of the B7 receptor CTLA-4 (CTLA-4-Ig). B7 gene expression and binding of anti-B7 antibodies and CTLA-4-Ig increased after treatment with interferom B7 was not inducible in human astrocytes. Human microglia expressed other costimulatory molecules, such as intercellular adhesion molecule-I, LFA-I and LFA-3. In sections of multiple sclerosis brains, B7 immunoreactivity was detected on activated microglia and infiltrating macrophages within active lesions. In chronic lesions, only perivascular cells were stained. B7 immunoreactivity was undetectable in sections from Alzheimer's disease or normal brain tissue. These data suggest that B7 may be involved in T cell activation and lesion development in multiple sclerosis and that the regulated expression of B7 on microglia may contribute to the local stimulation of T cell proliferation and effector functions.
Validation of intratracheal instillation of lung tumour cells in mice using single photon emission computed tomography/computed tomography imaging

In search of better predictive animal models for evaluating treatment response in lung cancer, orthotopic lung tumour models are a great step forward over traditional subcutaneous models. Crucial in the development of such orthotopic models is a reliable and reproducible instillation method. Because cells are instilled inside the thorax, the accuracy of the instillation and visualization of tumour growth demands the use of non-invasive imaging methods. We used a minimally invasive intratracheal intubation method to instill bioluminescent lung tumour cells in the lung parenchyma. Adaptation of the cell containing medium provides the possibility of tracing the exact location of the injection by means of single photon emission computed tomography/computed tomography (CT) imaging. The transplantation medium was also optimized to prevent migration of the injected substance. This results in the outgrowth of single and well-defined lung tumours at the instillation site. Finally, tumour growth was validated and longitudinally monitored with a combination of CT and bioluminescence imaging. The reported transplantation procedure enables the assessment of injection accuracy and provides a good approach for the generation of orthotopic lung tumour models for future response imaging studies.
Mammary Lobulo-Alveolar Growth Induced by Anterior Pituitary Hormones in Adreno-Ovariectomized and Adreno-Ovariectomized-Hypophysectomized Rats.∗ †

Summary 1. Mature virgin female Car-worth rats of the CFN strain were adreno-ovariectomized and were then injected thrice daily for 10 days with saline, 2 mg bovine STH, 30 I.U. ovine prolactin, 20 mg bovine anterior pituitary powder, or with a combination of 1.33 mg bovine STH and 20 I.U. ovine prolactin. Treatment with STH and prolactin together or with anterior pituitary powder induced moderate to extensive mammary duct and lobulo-alveolar growth, while treatment with prolactin and STH alone induced mainly duct and end bud development. 2. Two groups of rats were hypophysectomized and 3 days later were adreno-ovariectomized. They were then injected thrice daily for 10 days with saline or with a combination of 1.33 mg STH and 20 I.U. prolactin for 10 days. The latter treatment induced extensive duct and moderate lobulo-alveolar growth. Completeness of ovariectomy, adrenalectomy and hypophysectomy was checked in all rats upon autopsy. Sixteen additional adreno-ovariectomized rats which were given tap water failed to survive more than 19 days. 3. It is concluded that under conditions of this experiment, full mammary duct and lobulo-alveolar growth was induced by combined treatment with bovine STH and ovine prolactin in the absence of ovaries and adrenals. Addendum. Histological examination of stained mammary sections from adreno-ovariectomized rats treated with prolactin and STH failed to reveal any secretory activity. The authors are indebted to Mr. T. F. Hopkins for performing the hypophysectomies and to Mr. C. S. Nicoll for the photographs.
Enterprise A rchitecture M anagement ’ s I mpact on I nformation T echnology S uccess

Both practitioners and researchers put forward enterprise architecture management as a mean for achieving success with information technology. Many arguments have been put forward to support the benefits claimed to arise from mature enterprise architecture management and a considerable amount of literature describes the components of mature (successful) enterprise architecture management. However, few studies have empirically tested whether the enterprise architecture management activities impact organizations’ success with information technology. This paper tests the relationship between organizations’ success with information technology and enterprise architecture management activities. Significant correlations are found between these
Second-Order Procedure for Modeling Dielectric Material Interfaces in Structured Nonorthogonal Finite-Difference Discretizations of Maxwell's Equations

Structured nonorthogonal finite-difference discretizations of Maxwell's equations are often employed to incorporate curved dielectric material interfaces. As part of this process, it is necessary to employ an adequate procedure to enforce the tangential field continuity conditions at the locations of the interfaces where the components of the permittivity tensor are discontinuous. This contribution proposes a second-order domain-splitting procedure that can accomplish this task without assuming that the permittivity tensor components are piecewise constant or diagonal. As explained, this feature is needed to exploit the full geometrical flexibility of structured grid generation. The proposed domain-splitting procedure consistently yields a global error that is second-order accurate even in extreme cases where the components of the permittivity tensor have a change of sign or a sharp jump.
The measurement of denial and rationalization in male alcoholics.

Two hundred male alcoholics were given 94 true-false items constructed to characterize alcoholic defensiveness, and a factor analysis revealed two strong factors. The first factor (Denial) was correlated with items that asserted an ability to control one's drinking, denied being an alcoholic or needing treatment, or minimized the consequences of alcohol abuse. Items that loaded the other factor (Rationalization) gave reasons, justifications, and excuses for drinking. Two derived scales were examined in a replication sample of 66 male alcoholics, and alpha coefficients (.84 and .85) from the first sample did not show undue shrinkage in the second (.86 and .77).
Asymptotic Spectral Efficiency of Digital Transmission via Overcomplete Frames With Discrete, Finite, and Uniform Alphabets

It is recognized that, for digital symbols taken from a discrete and finite alphabet, perfect transmultiplexing at a signaling rate larger than the Nyquist rate can be achieved by modulation with overcomplete frame pulses. By invoking the spectral distribution theory of large random matrix, the spectral efficiency of digital transmission scheme via overcomplete frames in band-limited additive white Gaussian noise (AWGN) channel with discrete, finite, and uniform alphabets is investigated in this paper. It is shown that the proposed digital signaling scheme can asymptotically achieve the maximum spectral efficiency dictated by the Shannon capacity theorem for reliable transmission without employing signal shaping techniques. The extension to the case of non-white Gaussian noise/spectrally shaped channels is also considered. It is shown that the employment of Weyl-Heisenberg frames facilitates the optimal “water-filling” power allocation and rate adaptation by adjusting the signal amplitudes and energy-dispersion factors. Some numerical results are provided to support the theoretical finding.
In vitro and in vivo anticancer action of Saquinavir-NO, a novel nitric oxide-derivative of the protease inhibitor saquinavir, on hormone resistant prostate cancer cells

The NO-derivative of the HIV protease inhibitor saquinavir (Saq-NO) is a nontoxic variant of the parental drug with enhanced anticancer activity on several cell lines. However, it is still unclear whether the p53 status of the target cell might influence the sensitivity to Saq-NO. In this study we evaluated the in vitro and in vivo activity of Saq-NO on the p53-deficient hormone resistant prostate cancer PC-3 cells. We demonstrate that the absence of functional p53 is not essential for the capacity of Saq-NO to reduce prostate cancer cell growth. In contrast to its previously described cytostatic action in B16 and C6 cell lines, Saq-NO exerted cytotoxic effects in PC-3 cells leading to dominant induction of apoptosis and enhanced production of proapoptotic Bim. In addition, differently from saquinavir, Saq-NO restored TRAIL sensitivity that was correlated with increased expression of DR5 independent from ROS/RNS production and YY1 repression. NF-κB activation may be responsible of the Saq-NO induced DR5 expression. Moreover, Saq-NO but not saquinavir, exerted synergistic activity with conventional cytostatic therapy. In agreement with these in vitro studies, Saq-NO inhibited the in vivo growth of PC-3 cells xenotransplants to a greater extent than the parental compound. Taken together, these data indicate that Saq-NO possesses powerful and suitable in vitro and in vivo chemotherapeutic potential to be further studied as a novel drug for the treatment of prostate cancer in the clinical setting.
Nested harmonic broadcasting for scalable video over mobile datacast channels

The integration of reliable Video-on-Demand (VoD) broadcasting schemes in mobile datacast systems, specifically in DVB-H, is studied and enhanced. Sophisticated VoD broadcasting schemes such as Harmonic Broadcasting (HB) allows receivers to tune into the ongoing transmission of a video stream at arbitrary time, while still being able to receive the multimedia sequence from beginning to end, after short initial playout latency. In addition, we address service enhancements by using scalable video coding (SVC) to support heterogeneous receiver capabilities and receiving conditions as well as the reception of the signal from more than on transmission site. We present and discuss options for the integration of VoD broadcasting schemes in combination with fountain codes. Optimizations in parameter selection are discussed. A realistic protocol environment is only slightly modified to support our system concept. Simulation results show the benefits of the discussed VoD scheme compared to existing approaches if integrated in DVB-H.
European Society of Hypertension International Protocol revision 2010 for the validation of blood pressure measuring devices in adults

The Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Ireland, dabl Ltd., Blackrock Co., Dublin, Ireland, Hypertension Center, Third University Department of Medicine, Sotiria Hospital, Athens, Greece, Istituto Scientifico Ospedale San Luca, IRCCS, Instituto Auxologico Italiano, Milan, Italy, Societe Francaise d’Hypertension Arterielle, Filiale de la Societe Francaise de Cardiolgie, Paris, France, The Department of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Science and Medicine, Sendai, Japan, Centre for Epidemiological Studies and Clinical Trials, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China, University Clinic Bonn, Department of Internal Medicine, Bonn, Germany and Guy’s and St Thomas’ Hospitals, London, UK
Major bleeding after percutaneous coronary intervention and risk of subsequent mortality: a systematic review and meta-analysis

Objectives To examine the relationship between periprocedural bleeding complications and major adverse cardiovascular events (MACEs) and mortality outcomes following percutaneous coronary intervention (PCI) and study differences in the prognostic impact of different bleeding definitions. Methods We conducted a systematic review and meta-analysis of PCI studies that evaluated periprocedural bleeding complications and their impact on MACEs and mortality outcomes. A systematic search of MEDLINE and EMBASE was conducted to identify relevant studies. Data from relevant studies were extracted and random effects meta-analysis was used to estimate the risk of adverse outcomes with periprocedural bleeding. Statistical heterogeneity was assessed by considering the I2 statistic. Results 42 relevant studies were identified including 533 333 patients. Meta-analysis demonstrated that periprocedural major bleeding complications was independently associated with increased risk of mortality (OR 3.31 (2.86 to 3.82), I2=80%) and MACEs (OR 3.89 (3.26 to 4.64), I2=42%). A differential impact of major bleeding as defined by different bleeding definitions on mortality outcomes was observed, in which the REPLACE-2 (OR 6.69, 95% CI 2.26 to 19.81), STEEPLE (OR 6.59, 95% CI 3.89 to 11.16) and BARC (OR 5.40, 95% CI 1.74 to 16.74) had the worst prognostic impacts while HORIZONS-AMI (OR 1.51, 95% CI 1.11 to 2.05) had the least impact on mortality outcomes. Conclusions Major bleeding after PCI is independently associated with a threefold increase in mortality and MACEs outcomes. Different contemporary bleeding definitions have differential impacts on mortality outcomes, with 1.5–6.7-fold increases in mortality observed depending on the definition of major bleeding used.
Experimental verification of a modular multilevel cascade inverter based on double-star bridge-cells (MMCI-DSBC)

This paper presents a modular multilevel cascade converter based on double-star bridge-cells (MMCC-DSBC), which is characterized by a cascade connection of multiple single-phase full-bridge PWM converter cells per leg. It is suitable for a wind turbine generation system with a permanent magnet synchronous generator, the frequency band of which ranges typically from 5 to 20 Hz. This paper discusses a wind turbine generation system based on the DSBC, revealing that the DSBC can achieve a grid connection with an arbitrary dc-link voltage. Moreover, the operating principles and the control method are described in this paper. The validity of the system is verified experimentally by using a 200-V 10-kW downscaled model.