Miracle-dz/UniFAR-FTUs huggingface dataset:
Peroxisome-Proliferator Receptor Represses Hepatic Sex Hormone-Binding Globulin Expression

Plasma SHBG production by the liver is influenced by its metabolic state, and hepatocyte nuclear factor-4 regulates SHBG expression in response to changes in lipogenesis. Peroxisome-proliferator receptors (PPARs) also regulate glucose homeostasis and fatty acid metabolism. The human SHBG promoter contains a PPAR-response element (PPAR-RE), and plasma SHBG levels increase in polycystic ovarian syndrome patients treated with the PPAR agonist, rosiglitazone. In addition, plasma SHBG levels are associated with a genetic polymorphism in the PPAR -2 coding sequence that alters its transcriptional activity. Therefore, we set out to determine whether PPAR influences hepatic production of SHBG by using human HepG2 hepatoblastoma cells as an in vitro model. Surprisingly, treatment of HepG2 cells with rosiglitazone reduced SHBG production and SHBG promoter activity (as assessed in a luciferase reporter gene assay) by 20–25%, whereas the PPAR antagonist, GW9662, increased both by 2to 3-fold. The effects of PPAR agonists and antagonists on SHBG promoter activity were substantially diminished when the PPAR-RE in the SHBG promoter was mutated. A PPAR small interfering RNA also increased SHBG production by HepG2 cells as well as SHBG promoter activity, and the latter was accentuated by cotreatment with GW9662. Importantly, overexpression of a PPAR -2 Pro12 variant in HepG2 cells was more effective at reducing SHBG promoter activity, when compared with PPAR -2 Ala12, consistent with its superior PPAR-RE binding activity. We conclude that PPAR represses human SHBG expression in liver cells, and that differences in PPAR levels and activity contribute directly to variations in plasma SHBG levels. (Endocrinology 150: 2183–2189, 2009)
Chemotaxis to CX3CL1 (Fractalkine) Syk Is Required for Monocyte/Macrophage

CX3CL1 (fractalkine), the only member of the (cid:1) subclass of chemokines, is a known chemotactic factor for monocytes/macro-phages as well as NK cells and T lymphocytes. In several pathologies, excessive production of CX3CL1 at speciﬁc sites leads primarily to monocyte/macrophage recruitment, which causes tissue and vascular damage. Despite their clinical relevance, the mechanisms underlying monocyte/macrophage chemotaxis to CX3CL1 remain poorly documented. The present report addresses this issue and identiﬁes cell signaling crucial for this process. Using the murine monocyte/macrophage RAW cell line, we show that CX3CL1 treatment elicits a rapid and transient increase in F-actin and the formation of F-actin-enriched cell protrusions. CX3CL1 also triggers tyrosine phosphorylation of proteins localized in those protrusions. The protein tyrosine kinase Syk is activated upon CX3CL1 treatment, and reduction of Syk expression using RNA-mediated interference results in a speciﬁc and massive impairment of RAW cell migration to CX3CL1. Similar results are obtained using the Syk inhibitor, piceatannol. Cells with reduced Syk expression also exhibit a major defect in CX3CL1-induced cytoskeletal remodeling. These data suggest that in monocytes/macrophages, Syk is essential for proper reorganization of the actin cytoskeleton in response to CX3CL1 and is therefore required for cell chemotaxis to CX3CL1. The Journal of Immunology, 2005, 175: 3737–3745.
MODEL OF ELECTRICAL ACTIVITY OF THE BIOLOGIC NEURAL NETWORK AREAS

: In the paper, the mathematical model describing the generation of action potential and propagation of an impulse in the neuron's filaments on the basis of the analysis of parametric electri с circuits with distributed parameters and the mathematical model of synaptic interneuron connections are proposed. Developed models allow taking into account the influence of such factors as geometric, physical and chemical parameters of the neuron's filaments and the presence of different neurotransmitters in chemical synapses on transmitting a neural impulse. Further, such models can be used for investigating the conditions of neuron firing at spatial and time integration of input signals, as well as for the simulation of neuromuscular junctions.
Kobe University Repository : Kernel タイトル Tit le First report of Fusarium solani species complex as a causal agent of Erythrina variegata decline and death after gall format ion by Quadrast ichus erythrinae on Okinawa Island , Japan 著者

権利 Rights © The Author(s) 2017 This art icle is distributed under the terms of the Creat ive Commons Attribut ion 4.0 Internat ional License (ht tp://creat ivecommons.org/licenses/by/4.0/), which permits unrestricted use, distribut ion, and reproduct ion in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creat ive Commons license, and indicate if changes were made.
CMAR_A_234527 1419..1426

1Department of Oncology-Radiotherapy, Institute of Oncology Prof Dr Alexandru Trestioreanu, Bucharest, Romania; 2University of Medicine and Pharmacy Carol Davila, Bucharest, Romania; 3Department of Chemotherapy, OncoFort Clinic, Bucharest, Romania; 4Department of Medical Oncology, Antoine Lacassagne Cancer Center, Nice, France; 5Cancer Biology Transfer Platform, Georges Francois Leclerc Cancer Center, Dijon, France; 6Department of Medical Oncology, Georges Francois Leclerc Cancer Center, Dijon, France Introduction: Weekly paclitaxel (Ptx) and q3w docetaxel (Dtx) are equivalent in adjuvant breast cancer treatment. Weekly Ptx is better tolerated than q3w Dtx and became the first choice in daily practice, even preoperatively. Methods: To compare the efficacy and safety of the two regimens, a retrospective analysis was performed in breast cancer patients (pts) referred for neoadjuvant, sequential, taxanecontaining chemotherapy to the Institute of Oncology and Oncofort Clinic, Bucharest, between 2008 and 2017. Results: Forty-seven cases were eligible, median age was 56 years (34–73 years), mainly stage IIIA–B (53.2%, 25 pts) and ductal invasive (70.2%, 33 pts) of which 24 pts (51%) received q3w Dtx and 23 pts (48.9%) weekly Ptx. The histological response rates were 62.5% (15 pts) and 73.7% (17 pts) (p=0.47), average dose-intensity was 87.7% and 96.7% (p=0.002) and grade III–IV toxicity rate was 12.5% and 13% (p=0.64), respectively. Pathologic response was correlated with immunophenotype, PgR expression, tumor size and backbone chemotherapy (p<0.05). Discussion: Our study showed an improved efficacy of taxane’s weekly administration, probably due to a better tolerance and a lower rate of dose-impairing toxicities.
Essential for Viability

Legionella pneumophila, the causative agent of Legionnaires' disease, contains two superoxide dismutases (SODs), a cytoplasmic iron enzyme (FeSOD) and a periplasmic copper-zinc SOD. To study the role of the FeSOD in L. pneumophila, the cloned FeSOD gene (sodB) was inactivated with Tn903dlllacZ, forming a sodB::kacZ gene fusion. By using this fusion, expression of sodB was shown to be unaffected by a variety of conditions, including several that influence sod expression in Escherichia coli: aeration, oxidants, the redox cycling compound paraquat, manipulation of iron levels in the medium, and the stage of growth. A reproducible twofold decrease in sodB expression was found during growth on agar medium containing charcoal, a potential
PPA_A_276417 41..48

1Department of Dermatology, University Hospital Erlangen, Friedrich-AlexanderUniversity (FAU) Erlangen-Nuremberg, Erlangen 91054, Germany; 2Comprehensive Cancer Center Erlangen, European Metropolitan Region of Nuremberg, Erlangen, Germany; 3University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nuremberg (FAU), Erlangen 91054, Germany Purpose: An important measure of hospital quality is the satisfaction of the patients receiving in-patient care. This cross-sectional study aimed to assess skin cancer patients’ experiences in a university hospital setting as a measure of quality of cancer care. Patients and Methods: Questionnaires were mailed to patients with skin cancer after receiving in-patient overnight treatment in the dermatological unit of the university hospital Erlangen (Germany) from 1 September to 30 November 2017. Patients were asked to evaluate their overall experience of this episode of care and to complete the Picker Inpatient Survey questionnaire on specific aspects of their care, such as patient satisfaction regarding contact with staff, need for information, recommendation of the hospital as well as tumor-specific questions. The results were re-coded as problems and reported as frequencies and their percentage. Results: A total of 103 of 159 questionnaires were returned (64.8%). All patients rated the treatment and care they had received to be good or very good. Additionally, all patients would recommend our in-patient clinic to their families or friends. The patients most commonly criticized inconsistency of care delivered by the same physician (29.7%, 30/ 101) and feeling of insufficient involvement in the decision-making processes (21.1%, 20/ 95). Besides this, 19.0% (11/58) and 34.6% (18/52) of patients were not satisfied with physicians and nurses, respectively, appropriately addressing their fears or anxieties. In the cancer-specific questionnaire, the majority of patients were dissatisfied with further support regarding professional and social rehabilitation possibilities (85.7%, 30/35) and psychooncology (56.3%, 18/32). Conclusion: Overall, the majority of patients were satisfied with the in-patient skin cancer treatment. However, physicians and nurses can enhance patient satisfaction by addressing patients’ fears and anxieties regarding their disease and treatment. Besides, our results highlight the importance of psycho-oncological support.
IMMUNOMODULATORY EFFECTS OF THYME AND FENUGREEK IN SHARPTOOTH CATFISH, CLARIAS GARIEPINUS

The aim of this study was to investigate the modulation effects of thyme and fenugreek as food additives on sharptooth catfish immunity. Three diet regimes, a basic (control); Thyme diet (1%) and fenugreek diet (1%) were formulated and used to feed fish for 30 successive days. Half of the fish were used to investigate some of the immune parameters as differential leucocytic counts, serum globulins, phagocytic activities, phagocytic index, catalase and glutathione peroxidase. The other half of fish was subjected to challenge infection with Aeromonas hydrophila to investigate the disease resistance ability of the fish received the feeding additives. The results showed that, the serum globulins have significantly increased in fish fed with fenugreek, while the total serum protein has significantly increased in fish fed with thyme or fenugreek. Glutathione peroxidase (Gper) and catalase (CAT) increased significantly in the groups fed on fenugreek or thyme diets when compared to the control group. Phagocytic percent and index of fish fed on fenugreek were significantly higher than those of the control and thyme groups. Monocytes were significantly increased in all treated groups, while lymphocytes were significantly decreased in all treated groups. Weight gain of C. gariepinus was significantly lower in the group fed on thyme than in the control group, while it was insignificantly higher in fish fed fenugreek. After 30 days of feeding, fish were challenged with A. hydrophila, the cumulative mortalities were 40% and 26.67 % in fish fed on diet supplemented with 1% thyme and fenugreek, respectively, compared to 66.7% in the control group. It was concluded that thyme and fenugreek are be able to positively stimulate the immune system of C. gariepinus and decrease mortality rate in fish challenged with A. hydrophila.
OPTOELECTRONICS 2 . 1 μ m emission of Tm 3 + / Ho 3 + – doped antimony-silicate glasses for active optical fibre

Tm/Ho – doped antimony-silicate optical fibre with 2.1 μm emission has been presented. Luminescence corresponding to I7 → I8 transition in holmium was obtained by energy transfer between Tm and Ho ions. The analysis of the luminescence mechanism showed a significant influence of the glass composition (low phonon content) on the emission intensity. Optimization of the active elements content, presented in the paper, allowed to indicate that a strong emission intensity at 2 μm in the fabricated glasses was obtained for the molar composition of 1% Tm2O3 : 0.75% Ho2O3. According to the Förster-Dexter theory, the efficiency of energy transfer of the F4 (Tm) → I7 (Ho) transition was calculated. Moreover, it was found that the full width at half maximum (FWHM) of luminescence in the range of 1.6 – 2.2 μm strongly depends on the Tm/Ho ratio. The optimization of Tm/Ho transfer in antimony-silicate glasses allowed to fabricate optical fibre with narrowing and red-shifting of emission at 2.1 μm.
SEMI EMPIRICAL AM1 CALCULATIONS OF A FLAVONE: “5,6,7-TRIHYDROXY-4'-METHOXY FLAVONE”

Flavones are naturally occurring polyphenolic compounds known as nontoxic anti oxidants which mainly come from higher plants including seaweeds. These compounds possess a range of physiological properties and extensive literary information is available, but fewer theoretical insights of these compounds have been explored. In this paper, semi empirical Austin Model 1 (AM1) calculations has been reported for a flavone: “5,6,7-trihydroxy-4'-methoxy flavone” to predict the electronic structure and certain properties e.g. geometry optimization, total energy, dipole moments, and heat of formation etc.
Microsoft Word-foab029.docx

Glucose is a preferred carbon source for most living organisms. The metabolism and regulation of glucose utilization are well studied mostly for Saccharomyces cerevisiae. Xylose is the main pentose sugar released from the lignocellulosic biomass, which has a high potential as a renewable feedstock for bioethanol production. The thermotolerant yeast Ogataea (Hansenula) polymorpha, in contrast to S. cerevisiae, is able to metabolize and ferment not only glucose but also xylose. However, in non-conventional yeasts, the regulation of glucose and xylose metabolism remains poorly understood. In this study, we characterize the role of transcriptional factors Mig1, Mig2, Tup1 and Hap4 in the natural xylose-fermenting yeast O. polymorpha. The deletion of MIG1 had no significant influence on ethanol production either from xylose or glucose, however the deletion of both MIG1 and MIG2 reduced the amount of ethanol produced from these sugars. The deletion of HAP4-A and TUP1 genes resulted in increased ethanol production from xylose. Inversely, the overexpression of HAP4-A and TUP1 genes reduced ethanol production during xylose alcoholic fermentation. Thus, HAP4-A and TUP1 are involved in repression of xylose metabolism and fermentation in yeast O. polymorpha and their deletion could be a viable strategy to improve ethanol production from this pentose.
Creating Dynamic Robot Utterances in Human-Robot Social Interaction Comparison of a Selection-Based Approach and a Neural Network Approach on Giving Robot Responses in Conversations

Esoon Ko, Philip Andersson Denna studie undersöker två olika metoder för dialoghanteringssystem för att uppnå dynamiska yttrande i mänskliga-robot sociala interaktioner. Detta gjordes för att avgöra om Robot Assisted Language Learning (RALL) är lämplig som en lösning till den nuvarande situation i Sverige angående lärarbrist inom SFI. Ett tillvägagångssätt som tagits är ett urvalsbaserad med användandet av ett dialogträd med sentence embedding medan det andra tillvägagångssättet är genom Neural Network där två olika modeller tagits fram; en transformer modell och en seq2seq modell. Resultat från användartest visar att båda implementationerna inte var särskilt framgångsrika. Dock utfördes den urvalsbaserade metoden bättre än Neural Network metoder och är lovande för framtida forskning. På grund av att resultaten inte nått en tillfredsställande prestationsnivå och förekomsten av billigare virtuella utbildningsverktyg kanske inte är RALL den mest lämpliga lösningen för den nuvarande situationen i Sverige gällande lärarbrist inom SFI, men visar potential som en långsiktig lösning.
Tn5 Transposase-Mediated Mouse Transgenesis

We have developed a novel method for mouse transgenesis. The procedure relies on a hyperactive Tn5 transposase to insert a transgene into mouse chromosomes during intracytoplasmic sperm injection. This procedure integrates foreign DNA into the mouse genome with dramatically increased effectiveness as compared to conventional methods such as pronuclear microinjection and traditional sperm injection-mediated transgenesis. Our data indicate that with this method, transgenic mice, both hybrids and inbreds, can be produced more consistently and with lower numbers of manipulated oocytes required for traditional microinjection methods. The transposase-mediated transgenesis technique is also effective with round spermatids, offering the potential for rescuing the fertility of azoospermic animals using sperm precursor cells. oocyte development, sperm, spermatid
Characterization of factors in pathogenicity of Erwinia carotovora subsp. carotovora

Characterization of Factors Involved in Pathogenicity of Erwinia carotovora subsp. caroiovora May 1988 Franzine D. Smith, B.S., University of Vermont M.S., University of New Hampshire Ph.D., University of Massachusetts Directed by: Professor Mark S. Mount Erwinia carotovora subsp. carotovora (Ecc) strain EC 14 was subjected to transposon (Tn5) mutagenesis and the resultant mutants were screened on enzyme detection medium for production of pectolytic enzymes, cellulase, protease, phospholipase and DNase. Out of 6,637 mutants tested 36 showed changes in one or more enzyme activities. Two mutants were completely deficient in either DNase or protease activity. When maceration produced by the protease deficient mutant was compared to maceration of the wild type on potato slices, Ecc strain EC14 prt produced 6% less macerated tissue, but this difference was not significant above P=0.50. One major extracellular protease was isolated from Ecc strain EC14 using DEAE (DE53) cellulose column chromatography and isoelectric focusing. The extracellular protease had a pi = 4.95, a molecular weight of 53,000 and its optimum pH for activity was 7. Using a cAMP deficient mutant Ecc strain EC1491 it was determined that protease was not under cAMP control. A 2.5 kb piece of DNA from Ecc strain EC14 was subcloned from the hybrid protease cosmid pCA7 into pBR325. The resultant plasmid (pFSD produced protease in E. coli strain HB101. Plasmid pFSl was subjected to Tn5 transpositional mutagenesis and a protease deficient mutant was selected. Attempts to transform Ecc strain EC14 with plasmid pFSl::Tn5 were unsuccessful. Two Erwinia herbicola (EM and two E. coli strains were transformed with hybrid plasmids pDRl or pDR30 w hich contained pectolytic genes of Ecc strain EC14. When intracellular and extracellular
Variasi Morfologi Jambu Biji ( Psidium guajava L.) di Purwokerto

The common guava Psidium guajava L. has high variety, emerging from its leaf, tree trunk, seed, flower and fruit characteristic. This knowledge is in demand for potential exploration of the common guava. Two objectives were defined for this study. Firstly, it was to define morphological variation of the common guava P. guajava L. Secondly, it was to determine similarity and its relationship among the cultivars. This study conducted purposive sampling method to collect the samples. Samples were collected in Sumbang, Grendeng, and Pabuaran. Variabels such as morphological characteristic in the species tree, tree trunk, leaf, and fruit. The morphological variation was described descriptively. The relationship among the cultivars similarity was analyzed using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method. The result revealed that there are seven cultivars of the common guava P. guajava L. in Purwokerto area. Those cultivars are Psidium guajava ‘Merah’ , P. guajava ‘Putih’, P. guajava ‘Kristal Merah’, P. guajava ‘Kristal Putih’, P. guajava ‘Getas’, P. guajava ‘Bangkok’, dan P. guajava ‘Australi’. The similarity phenogram showed that the seven cultivars categorized into two main clusters. The Kristal Putih and Bangkok has the highest similarity, supported by its dissimilarity index of 0,333. The Kristal Merah is the least similar with the rest of the cultivars, especially with Getas and Merah, with dissimilarity index of 0,917.
COP 9 : A New Genetic Locus lnvolved in Light-Regulated Development and Gene Expression in Arabidopsis

We report here the identification and characteriration of a new Arabidopsis light-regulatory locus, COP9, mutation that leads to a constitutive photomorphogenic phenotype. Dark-grown cop9 seedlings exhibit many morphological characteristics of light-grown seedlings, including short hypocotyls and open and enlarged cotyledons with cell-type and chloroplast differentiation. Furthermore, the cop9 mutation leads to high-leve1 expression of light-inducible genes in the absence of light, probably by altering the promoter activitles of these genes. These properties imply that the mutation in the COP9 locus uncouples the llghtldark signals from morphogenesis and light-regulated gene expression. In addition, llght-grown cop9 mutants are severely dwarfed and are unable to reach maturation and flowering. This adultlethal phenotype lndlcates that the COP9 locus also plays a critical role for normal development of the light-grown plant. Similar to cop l mutants, but not d e f , the cop9 mutants show (1) no effect on the phytochrome control of seed germlnat lon and (2) deficiency in the dark-adaptive change of expression of light-regulated genes. Our results suggest that the cop9 and cop l mutatlons result in the same range of phenotypes and therefore COP9 and COPl loci may encode closely related components ln the same regulatory pathway.
RRU_A_259357 295..302

Urology Department, Ramón y Cajal University Hospital, Alcalá University, IRYCIS, Madrid, Spain Purpose: The objective is to present a case of well-leg compartment syndrome in the Galdakao-modified supine Valdivia position. Results: The case of a 32-year-old male, obese (105 Kg) and a former smoker is presented. The patient was positioned in the Galdakao-modified supine Valdivia position, with lower limbs bandaged, to perform a right percutaneous nephrolithotomy. In the immediate postoperative period, significant pain was reported in the left lower limb. The limb appeared oedematous and cyanotic, although pedis pulses were preserved. Doppler ultrasound ruled out venous thrombosis. Suspecting compartment syndrome, the patient underwent a complete decompression fasciotomy of the four left leg compartments. After the surgery, values of creatine phosphokinase reached 80.000 UI/L and serum creatinine levels were 1.53 mg/dL. The patient was taken to the intensive care unit. Six months after the episode, the patient still needs rehabilitation care. The compartment syndrome is a rare complication in lithotomy position, but never described in the Galdakao-modified supine Valdivia position before, with the lower limbs in moderate flexion, and with the ipsilateral lower limb in a slightly inferior position with respect to the other. It may lead to skin necrosis, permanent neuromuscular dysfunction, myoglobinuric renal failure, amputation and even death. Therefore, this complication must be suspected and early decompression of the compartment must be performed. Risk factors include obesity, peripheral vascular disease (advanced age, hypertension, hyperlipidemia and diabetes mellitus), height, hypothermia, acidemia, BMI, male sex, combined general-spinal anesthesia, prolonged surgery time, systemic hypotension, ASA (American Society of Anesthesiologists) class, lack of operative experience, vasoconstricting drugs, important bleeding during the surgery and increased muscle bulk. Conclusion: Compartment syndrome is a potentially life-threatening complication that may occur in the Galdakao-modified supine Valdivia position. It should be suspected in cases with risk factors and compatible clinical symptoms and signs, and treated rapidly to avoid further complications.