Miracle-dz/UniFAR-FTUs huggingface dataset:
Complete mitochondrial genome of the Southeast Asian shrew Crocidura fuliginosa (Soricomorpha: Soricidae)

Abstract The complete mitochondrial genome of the Southeast Asian shrew (Crocidura fuliginosa) was first sequenced and characterized. The genome was 16,967 base pairs (bp) in length containing 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and a control region. The composition and arrangement of genes were analogous to other vertebrates. All PCGs were used to construct phylogenetic tree for C. fuliginosa and other 12 Crocidura species available on GenBank. Phylogenetic result indicated that C. fuliginosa has a close relationship with C. lasiura and C. attenuate. Our results will provide information for further molecular studies about this genus.
Performance of nursery pigs fed diets with coated or potentiated zinc oxide.

A 30-d experiment was carried out to compare the effects of two zinc oxide sources on performance of nursery pigs. Four hundred sixty-one mixed-sex weanling pigs (5.34 ± 0.16 kg) were randomly allotted to two dietary treatments. Pigs were fed basal diet added with either 500 g/ton coated zinc oxide (cZnO) or 300 g/ton potentiated zinc oxide (pZnO). Each treatment was replicated three times with 76-77 pigs per replicate. Weighing was done three times at 15-d interval. Average daily gain of pigs fed diets supplemented with pZnO tend to be higher than those fed diets supplemented with cZnO at d 1-15 (0.16 vs 0.11 kg; p=0.09), d 16-30 (0.53 vs .41 kg; p=0.05), and at d 1-30 (0.34 vs 0.26 kg; p=0.06). There were no differences on ADFI (p=0.43) for each feeding phase and for the overall period. Feed conversion ratio (FCR) at d 1-15 did not differ between treatments (p=0.12), but pigs fed diets with pZnO improved by 22.07% at d 16-30 (1.73 vs 2.22; p=0.07) and for the overall period by 25.40% (1.88 vs 2.52; p=0.07). Furthermore, mortality was lower in pigs fed diets with pZnO (6.57 vs 15.86 %; p=0.01) than those fed with cZnO. Results indicated the advantages of pZnO over cZnO on growth and survivability of pigs.
Intellectual structure of multigenerational workforce and contextualizing work values across generations: a multistage analysis

PurposeThis study attempts to understand the research clusters and thematic evolution of the topic generational diversity at workplace, over the period of 2001–2009 and 2010–2018. Furthermore, it attempts to identify the key shifts (and convergence) that have taken place in the value system across generational cohorts.Design/methodology/approachIn this context, the first stage of the study involved an in-depth systematic analysis of extant literature on multigenerational workforce between 2001–2009 and 2010–2018 by applying bibliometric analysis. Following an explanatory mix-method approach, the second stage of the study comprised of 32 interviews conducted across generations, exploring the role of ethics at the workplace.FindingsIt was revealed that during the period 2001–2009, communication and identification of generational characteristics emerged as the major themes. The 2010–2018 period unraveled four themes of research – retaining and engaging millennials through leadership, generational differences in work values, impact of generational differences on organizational-level variables and generational diversity in education and nursing. The outcome from the second stage showed that work values differ across generations with an emphasis on intrinsic work values, and work values have rather deteriorated, with baby boomers possessing stronger work ethics in comparison to the millennials. Finally, an integrated model for multigenerational workforce has been proposed.Research limitations/implicationsThis paper provides significant inputs to the expanding research in the area of work values, as it delves into the principal mechanisms leading to differences in work values among generations.Originality/valueBibliometric analysis, which is a quantitative approach to understanding the intellectual structure of a research topic, has been applied to generational diversity at the workplace. This constitutes a novel attempt that can be bracketed as a pertinent contribution to the field.
Study the Effect of Total Carotenoids from Sporidiobolus pararoseus on Acute Lung Inflammation in Mice Exposed to Cigarette Smoke

Cigarette smoke (CS) is a major risk for chronic obstructive pulmonary disease (COPD). Pathogenesis hallmarks in the lungs of COPD patients always contribute to causing inflammation and oxidative stress. Carotenoids, extracted from Sporidiobolus pararoseus, have antioxidant and antitumor effect. These carotenoids have a similar structure to lycopene, which is demonstrated effective in the treatment of lung inflammation. This presented work aims to investigate the anti-inflammatory and antioxidant functions of total carotenoids (TC), extracted from Sporidiobolus pararoseus, on mice under a short-term exposure of cigarette smoke. Forty-eight C57BL/6 male mice were divided into 6 groups (n=8): first group was control group exposed to ambient air, second group was CS group exposed to CS, third group was CS+LY18 group exposed to CS and treated with lycopene 18 mg/kg (CS+LY18), other groups (CS+TC18, CS+TC12, CS+TC9) exposed to CS and treated with (TC) at different doses (18, 12, 9 mg/kg) respectively. Treatment with TC especially at high dosage 18 mg/kg led to decrease the levels of MDA, GSH, activities of CAT, SOD in lung samples, as well as TNF-α and IL-6 levels in lungs and bronchoalveolar lavage fluid. In addition, it attenuated the morphological changes in the lungs. The high dose of TC showed a stronger effect than lycopene. These results demonstrated that TC from Sporidiobolus pararoseus have effective functions for acute lung inflammation induced by CS, which suggested a positive intervention for the treatment of COPD.
Detecção e identificação de espécies de Rickettsia em carrapatos coletados de aves silvestres no Brasil pela PCR-RFLP

ABSTRACT. Santolin I.D. A.C. , Famadas K.M. & McIntosh D. Detection and identification of Rickettsia agents in ticks collected from wild birds in Brazil by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. [Deteccao e identificacao de especies de Rickettsia em carrapatos coletados de aves silvestres no Brasil pela PCR-RFLP. ] Revista Brasileira de Medicina Veterinaria, 35(Supl.2):68-73, 2013. Programa de Pos-Graduacao em Ciencias Veterinarias, Instituto de Veterinaria, Anexo 1, Universidade Federal Rural do Rio de Janeiro, Campus Seropedica, BR 465 Km 7, Seropedica, RJ 23897-970, Brasil. E-mail: isis@santolin.com. br The detection and identification of Rickettsia bacteria in tick populations have become easier and more precise during the last 25 years as result of the developmental of robust molecular biology based methods. Currently, seven species of Ricketssia have been described in Brazilian ticks, using PCR for detection and amplicon sequencing for identification. In silico analysis of sequences encoding the gltA, htrA and ompB genes of those species was performed and revealed the basis for a novel PCR-RFLP method that allows their differential identification. The method was evaluated using larvae and nymphs of Amblyomma longirostre, A. ovale and A. varum collected from birds in the Tingua Biological Reserve, in Rio de Janeiro State. The species “Candidatus Rickettsia amblyommii” was identified in 100% of the A. longirostre examined, while the other two tick species were PCR negative. The basic method employs the restriction endonucleases MspI and RsaI and can be performed in the course of a single working day. It offers a convenient and cost effective means to perform large scale analysis of tick populations, and should be of benefit to researchers who lack the financial or technical resources necessary for sequence based identification.
The Impact of Gender Differences on the Expressions of Refusal in Jordanian Arabic

The present study investigates the use of the expression of refusal by native speakers of Jordanian Arabic (NSsJA) in different social situations (i.e. invitations, suggestions, and offers). It also investigates the influence of gender on the refusal realization patterns within the Jordanian culture to provide a better insight into the relation between situations, strategies and gender in the Jordanian culture. To that end, a group of 70 participants, including 35 male and 35 female students from different departments at the Hashemite University (HU) participated in this study using mixed methods (i.e. Discourse Completion Test (DCT), interviews and naturally occurring data). Data were analyzed in light of a developed coding scheme. The results showed that NSsJA preferred indirect strategies which mitigate the interaction such as "excuse, reason and, explanation" strategy more than other strategies which aggravate the interaction such as "face-threatening" strategy. Moreover, the analysis of this study has revealed a considerable impact of gender on the use of linguistic forms expressing refusal among NSsJA. Significant differences in the results of the Chi-square test relating the effect of participants' gender indicate that both males and females were conscious of the gender of their interlocutors. The findings provide worthwhile insights into the relation amongst types of communicative acts and the rapport between people in social interaction. They assert that refusal should not be labeled as face threatening act since it does not always pose a threat in some cases especially where refusal is expressed among friends, relatives and family members. They highlight some distinctive culture-specific features of the communicative acts of refusal. Keywords—Speech act, refusals, semantic formulas, politeness, Jordanian Arabic, mixed methodology, gender.
Multi-plane Imaging of Neural Activity From the Mammalian Brain Using a Fast-switching Liquid Crystal Spatial Light Modulator

We report a novel two-photon fluorescence microscope based on a fast-switching liquid crystal spatial light modulator and a pair of galvo-resonant scanners for large-scale recording of neural activity from the mammalian brain. The utilized imaging technique is capable of monitoring large populations of neurons spread across different layers of the neocortex in awake and behaving mice. During each imaging session, all visual stimulus driven somatic activity could be recorded in the same behavior state. We observed heterogeneous response to different types of visual stimuli from ~ 3,300 excitatory neurons reaching from layer II/III to V of the striate cortex.
Content Analysis in Business Digital Media Columns: Evidence From Colombia

ABSTRACT The business world is a voracious consumer of business media content. Research on business columnists has been focused mainly on the effects of columns’ content on stock prices and stock exchange performance indicators. In this study, we delve into the characteristics of business media and their development in terms of the readability and content of business digital media columns from a developing country (Colombia). For this, we conducted a readability and content analysis of a sample of 565 columns from Dinero and Portafolio, two of the most influential business media outlets in Colombia. Our results showed an over-representation of male columnists, a lower readability compared with columnists from abroad, and non-significant differences in readability regarding the media outlet and the columnists’ academic degree or gender. We also found that both media outlets have similar patterns in terms of their narratives.
Ecological Dynamics: An Inspiration for Triggering Epilepsy

Epilepsy is a rigorous transmission of electrical impulses across neurons of the brain and reported more prevalent in lower-income countries. A systematic literature review has been performed to implicate the impact of environmental variables on the occurrence of epilepsy using the following keywords: Epilepsy or environmental risk factors or seizures. More than 500 genes have been reported to involve in epilepsy potentially. Moreover, family history with neurological disorders, sleep apnea, depression, alcohol, stress, diet, gestational period of mother, and social involvement are among the risk factors which can reliably predict onset and severity of the disease. Hence, minimizing these factors along with recommended therapies, counseling, and awareness could be a miracle in the life of epileptic patients and can improve societies.
GPER mediates the age-dependent upregulation of the myocardial endothelin system

Aims—Cardiac aging is associated with progressive structural changes and functional impairment, such as left ventricular hypertrophy, fibrosis and diastolic dysfunction. Aging also increases myocardial activity of endothelin-1 (ET-1), a multifunctional peptide with growthpromoting and pro-fibrotic activity. Because the G protein-coupled estrogen receptor (GPER) regulates vascular responsiveness to ET-1, we investigated whether GPER also plays a role in the regulation of the cardiac endothelin system with aging. Main methods—Young (4 month-old) and aged (24 month-old) wild-type and Gper-deficient (Gper-/-) mice were studied. Gene expression levels of prepro-ET-1, endothelin converting enzymes ECE-1 and ECE-2, and endothelin ETA and ETB receptors were determined by qPCR in left ventricular myocardium. Key findings—Aging markedly increased steady-state mRNA expression levels of ECE-1, ECE-2, ETA and ETB receptors (each p<0.001 vs. young mice). Deletion of Gper inhibited the age-dependent increase in ECE-2 and ETB receptor mRNA levels (57% and 40% reduction, respectively, each p<0.01 vs. wild-type mice), whereas gene expression of prepro-ET-1, ECE-1, or the ETA receptor was unaffected in Gper-/mice. Significance—We identified a novel regulatory mechanism through which the endogenous Gper facilitates the age-dependent increase in myocardial expression of ECE-2 and the ETB receptor, which is compatible with an activating role of GPER for the cardiac endothelin system with aging. Correspondence should be addressed to: Matthias R. Meyer, M.D. Department of Cardiology, Cantonal Hospital, Tellstrasse, 5001 Aarau, Switzerland, Tel: +41 62 838 4483, Fax: +41 62 838 4299, matthias.meyer@ksa.ch; or Eric R. Prossnitz, Ph.D. University of New Mexico Health Sciences Center, Department of Internal Medicine, 2325 Camino de Salud NE, Albuquerque, NM 87131, United States, Tel: +1 505 272 5647, Fax: +1 505 272 1421, eprossnitz@salud.unm. edu. *Current address: Department of Cardiology, Cantonal Hospital, Aarau, Switzerland Financial Disclosure and Competing Interests Statement: M.R. M. , G.S. , M.B. , and E.R. P. are inventors on U.S. patent applications for therapeutic use of compounds targeting GPER. E.R. P. is an inventor on U.S. patent Nos. 7,875,721 and 8,487,100 for GPERselective ligands and imaging agents. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHS Public Access Author manuscript Life Sci. Author manuscript; available in PMC 2017 August 15. Published in final edited form as: Life Sci. 2016 August 15; 159: 61–65. doi:10.1016/j.lfs. 2016.02. 041. A uhor M anscript
Attacks detection and decision framework based on generative adversarial network approach: Case of vehicular edge computing network

The application of artificial intelligence in cyber security context has attracted enormous attention, specifically from industrial sector and this is due to the strength of machine learning algorithms to detect the unknown misbehaviors executed by cyber‐attacks. The generative adversarial network (GAN) based on a generator and discriminator systems could be used as defense and attack frameworks to protect and attack respectively its target. In this research article, we propose new attacks detection and decision framework based on GAN algorithm to detect accurately the smart and dangerous attacks. The proposed security framework relies on the collaboration between the generator and discriminator systems to determine the relevant attacks' features and hence detect the cyber‐attacks with a high accuracy. We present a case study of the proposed detection and decision framework in a context of a vehicular edge computing network and highlight the experimental results by analyzing specifically, the accuracy defense rate and network latency. In the experimentation results, a maximum number of network attacks are detected with a high accuracy, while low network latency is generated. This result is achieved specifically when the number of iterations is high, that is, the accuracy defense rate is close to 90% and the network latency is close to 1000 milliseconds.
Precise Single-Frequency Positioning Using Low-Cost Receiver with the Aid of Lane-Level Map Matching for Land Vehicle Navigation

Precise positioning with low-cost single-frequency global navigation satellite system (GNSS) receivers has great potential in a wide range of applications because of its low price and improved accuracy. However, challenges remain in achieving reliable and accurate solutions using low-cost receivers. For instance, the successful ambiguity fixing rate could be low for real-time kinematic (RTK) while large errors may occur in precise point positioning (PPP) in some scenarios (e.g., trees along the road). To solve the problems, this paper proposes a method with the aid of additional lane-level digital map information to improve the accuracy and reliability of RTK and PPP solutions. In the method, a digital camera will be applied for lane recognition and the positioning solution from a low-cost receiver will be projected to the digital map lane link. With the projected point position as a constraint, the RTK ambiguity fixing rate and PPP performance can be enhanced. A field kinematic test was conducted to verify the improvement of the RTK and PPP solutions with the aid of map matching. The results show that the RTK ambiguity fixing rate can be increased and the PPP positioning error can be reduced by map matching.
Using Waste in Producing Bio-Composite Mycelium Bricks

One of the major causes of an increase in the consumption of resources is the progress of the construction industry. Although it leads to new technologies, it heavily contributes to global warming. In this study, the use of sustainable construction materials from waste in brick production with mycelium as a binder is investigated. The ability of mycelium, the root fibers of fungi, obtained from microorganisms is used as stabilizing and binding material on bricks. Forty-eight brick specimens from six design mixes were produced with a size of 200 mm length × 90 mm width × 60 mm height. The mechanical tests conducted were compressive and flexural strength. The changes in weight were recorded against its age to monitor the progress of mycelium growth inside the brick specimens. From the test, bricks made from sawdust and rice bran with mycelium had an increase of 31.0% to 38.5% in average compressive strength compared to the non-mycelium bricks, respectively. Furthermore, the bricks with mycelium experienced an increase in both flexural strength and midpoint displacement for all types of bricks (rice bran, sawdust, and clay). These mycelium-induced bricks can reduce the use and consumption of traditional construction materials with enhanced mechanical properties.
Proteolysis of the reveise transcriptase of hepatitis B virus by ion protease in E coli

Hepatitis B virus (HBV) polymerase, which possesses the activities of terminal binding, DNA polymerase, reverse transcriptase and RNaseH, has been shown to accomplish viral DNA replication through a pregenomic intermediate. Because the HBV polymerase has not been purified, the expression of HBV polymerase was examined in an E. coli expression system that is under the regulation of arabinose operon. The expressed individual domain containing terminal binding protein, polymerase, or RNaseH turned out to be insoluble. The activities of those domains were not able to be recovered by denaturation and renaturation using urea or guanidine‐HCl. The expressed reverse transcriptase containing the polymerase and RNaseH domains became extensively degraded, whereas the proteolysis was reduced in a lon‐ mutant. These results indicate that Lon protease proteolyzes the HBV reverse transcriptase expressed in E. coli.
Molecular identification of fungal species from deteriorated old Chinese manuscripts in Central Library Universitas Indonesia

Diverse fungi were the potential cause of deterioration of written heritage. The old Chinese manuscripts from Central Library Universitas Indonesia showed biodeterioration by fungi. This study was conducted to identify eleven fungal strains from four deteriorated old Chinese manuscripts from Central Library UI by molecular identification using nucleotide sequences of the ITS regions of ribosomal DNA. PCR and DNA sequencing were performed using forward primer ITS5 and reverse primer ITS4. Homology search of ITS rDNA sequences was carried out using basic local alignment search tool (BLAST) program. Full sequences of fungal strain ITS rDNAs were obtained from contig sequences using Chromas Pro 1.7. 7 application. Only two fungal strains could be determined to species level. UICC 1099 strain showed 99.4% homology to one type strain, Aspergillus pseudodeflectus NRRL 6135T. UICC 1101 strain showed 99.8 % homology to one type strain, Penicillium coffeae NRRL 35363T. Nine strains showed homology (85–100%) to more than one type strain as follows: UICC 1100 strain showed homology greater than 99% to three type strains of Aspergillus. UICC 1102 strain showed homology greater than 99% to two type strains of Aspergillus. UICC 1103 strain showed homology greater than 99% to five type strains of Cladosporium. UICC 1104 strain showed homology (92–97%) to three type strains of Sarocladium and Phaeoacremonium. UICC 1105 showed homology (92–93%) to four type strains of Curvularia and Bipolaris. UICC 1106 strain showed homology (99–100%) to five type strains of Cladosporium. UICC 1107 showed homology (95–99%) to three type strains of Paecilomyces and Purpureocillium. UICC 1108 strain showed homology (92–94%) to four type strains of Curvularia and Bipolaris. UICC 1109 strain showed homology (85%) to four type strains of Fusarium and Rectifusarium. Molecular phylogenetic analysis is required to determine the species identities of these strains.Diverse fungi were the potential cause of deterioration of written heritage. The old Chinese manuscripts from Central Library Universitas Indonesia showed biodeterioration by fungi. This study was conducted to identify eleven fungal strains from four deteriorated old Chinese manuscripts from Central Library UI by molecular identification using nucleotide sequences of the ITS regions of ribosomal DNA. PCR and DNA sequencing were performed using forward primer ITS5 and reverse primer ITS4. Homology search of ITS rDNA sequences was carried out using basic local alignment search tool (BLAST) program. Full sequences of fungal strain ITS rDNAs were obtained from contig sequences using Chromas Pro 1.7. 7 application. Only two fungal strains could be determined to species level. UICC 1099 strain showed 99.4% homology to one type strain, Aspergillus pseudodeflectus NRRL 6135T. UICC 1101 strain showed 99.8 % homology to one type strain, Penicillium coffeae NRRL 35363T. Nine strains showed homology (85–100%) to more .