Miracle-dz/UniFAR-FTUs huggingface dataset:
A tale of worldwide success: Behind the scenes of Carex (Cyperaceae) biogeography and diversification

The megadiverse genus Carex (c. 2000 species, Cyperaceae) has a nearly cosmopolitan distribution, displaying an inverted latitudinal richness gradient with higher species diversity in cold‐temperate areas of the Northern Hemisphere. Despite great expansion in our knowledge of the phylogenetic history of the genus and many molecular studies focusing on the biogeography of particular groups during the last few decades, a global analysis of Carex biogeography and diversification is still lacking. For this purpose, we built the hitherto most comprehensive Carex‐dated phylogeny based on three markers (ETS–ITS–matK), using a previous phylogenomic Hyb‐Seq framework, and a sampling of two‐thirds of its species and all recognized sections. Ancestral area reconstruction, biogeographic stochastic mapping, and diversification rate analyses were conducted to elucidate macroevolutionary biogeographic and diversification patterns. Our results reveal that Carex originated in the late Eocene in E Asia, where it probably remained until the synchronous diversification of its main subgeneric lineages during the late Oligocene. E Asia is supported as the cradle of Carex diversification, as well as a “museum” of extant species diversity. Subsequent “out‐of‐Asia” colonization patterns feature multiple asymmetric dispersals clustered toward present times among the Northern Hemisphere regions, with major regions acting both as source and sink (especially Asia and North America), as well as several independent colonization events of the Southern Hemisphere. We detected 13 notable diversification rate shifts during the last 10 My, including remarkable radiations in North America and New Zealand, which occurred concurrently with the late Neogene global cooling, which suggests that diversification involved the colonization of new areas and expansion into novel areas of niche space.
Association of Resistin Gene Polymorphism with Type 2 Diabetes in Khuzestan Province

Background: Genome-Wide Association Studies (GWAS) have recognized that polymorphisms of the resistin gene were strongly correlated with the risk of type 2 diabetes. Objectives: The present study aimed to investigate the association of the -420 resistin gene polymorphism with type 2 diabetes in Khuzestan province, Iran. Methods: Unrelated healthy controls (n = 200) and type 2 diabetes mellitus (T2DM) patients (n = 200) were selected from Khuzestan province, Iran. Genotyping was performed by the PCR-RFLP method. Results: The frequency of the CC genotype in diabetic patients was about twice as high as in healthy individuals (P < 0.05). The frequency of the C allele was higher in diabetics than in healthy subjects and this difference was statistically significant. Also, diabetic patients with the CC genotype had the highest fasting blood glucose and the lowest HbA1C among studied patients with other genotypes although these differences were not statistically significant (P ≥ 0.05). Conclusions: The findings showed that the resistin gene polymorphism at position -420 (C>G) gene was correlated with T2DM. In addition, the high frequency of the C allele in diabetic patients may influence susceptibility to T2DM.
The complete mitochondrial genome analysis of the stonefly, Flavoperla sp. (Plecoptera: Perlidae)

Abstract Flavoperla is a small genus in Acroneuriinae, which was established by Chu but was treated as a synonym for a long time until Uchida and Harper recognized Flavoperla as a valid genus. This genus includes 15 species in the world now, including six species from China. Here, we sequenced and annotated the mitochondrial genome of Flavoperla sp. under the next-generation sequence technology, the first representative of Genus Flavoperla. The complete mitochondrial genome of Flavoperla sp. is 15,796 bp in length with an A + T content of 68.3% showed a positive AT-skew (0.037) and a negative GC-skew (−0.262). The gene order and organization of the mitochondrial genome is consistent with other stoneflies. The control region had the highest A + T content. Eleven PCGs started with the typical codon (ATN), the remaining PCGs started with TTG (ND1and ND5). All PCGs terminated with TAA/TAG, except COII and ND5 used single T. The phylogenetic tree by maximum likelihood (ML) and Bayesian (BI) methods supported that Flavoperla sp. was the sister group to Niponiella limbatella.
First record of the mustelid Trochictis (Carnivora, Mammalia) from the early Late Miocene (MN 9/10) of Germany and a re-appraisal of the genus Trochictis

ABSTRACT We present the first record of the mustelid Trochictis from the Late Miocene (MN 9/10) of Germany, a partial mandible with p4, m1, and m2 from Eppelsheim. Trochictis peignei sp. nov. is characterised by the combination p4 with a distal accessory cuspid and a basal lingual enlargement, m1 with a length/width index larger than 2.5, a rounded lingual wall of the paraconid, an entoconulid present, the metaconid as high as the paraconid, and cuspules present on the posterior talonid edge, and m2 with a very reduced talonid. Comparison to similar sized Middle and Late Miocene mustelids and a stratocladistic analysis place T. peignei sp. nov. closest to T. narcisoi from MN 9 of Can Llobateras, T. depereti from several MN 6 to MN 7/8 European localities, and cf. Trochictis sp. from MN 9 of Rudabánya. We also suggest synonymy of T. carbonaria and T. artenensis and verify that m2 of Trochictis occasionally is double-rooted. The analysis does not corroborate a close relationship of Trochictis to Taxodon or the subfamily Ictonychinae, but can also not discard confidently such an assignment. With a body mass of about 3 kg, Trochictis peignei sp. nov. represents the hitherto smallest described carnivoran of the Eppelsheim Formation.
Variabilidad isoenzimatica morfológica y selección clonal en Castanea sativa miller c. Crenata sieb. E zucc., c. Mollisima e híbridos interespecificos

Se identifica material clonal de castano resistente a phytophthora sp. Introducido en el c.I. F. De lourizan antes de 1990, seleccionado el mas interesante para la produccion de madera. Se ha trabajado con materiales hibridos, fundamentalmente entre c. Crenata y c. Sativa. Se ha empleado el analisis de isoenzimas para la identificacion de clones, especies e hibridos, debido a la existencia de varios loci diagnostico entre especies en aconitasa, fosfoglucoisomerasa, fosfoglucomutasa y shikimatodeshidrogenasa, asi como al polimorfismo existente en c. Sativa en fosfoglucoisomerasa y ugp. Los analisis de la variabilidad genetica de marras, caracteres de vigor y calidad de fustes han indicado que para evitar la disminucion de la heredabilidad clonal en vigor y marras, es necesario trabajar con planta de elevada calidad exterior. Se discute el resultado de la seleccion clonal realizada con diferentes criterios.
Scalability and Performance Efficiency of History Matching Workflows using MCMC and Adjoint Techniques Applied to the Norne North Sea Reservoir Case Study

Summary An increasing number of field development projects include rigorous uncertainty quantification workflows based on parameterized subsurface uncertainties. Reservoir model calibration workflows for reservoir simulation models including historical production data, also called history matching, deliver non-unique solutions and remain technically challenging. In addition, the validation process of the reservoir simulation model often introduces a break of the conceptual connection to the geological model. This raises questions on how to quantify the deviation between the calibrated simulation model and the original geological model. Workflow designs for history matching require scalable and efficient optimization techniques to address project needs. Derivative-free techniques like Markov Chain Monte Carlo (MCMC) are used for optimization and uncertainty quantification. Adjoint techniques derive analytical sensitivities directly from the flow equations. For history matching those sensitivities are efficiently used for property updates on grid block level. Both techniques have different characteristics and support alternative history matching strategies like global vs. local, stochastic vs. deterministic. In this work both techniques will be applied in an integrated workflow design to the Norne field. The Norne field is a North Sea oil-and-gas reservoir with approximately 30 wells, with one third being used for WAG injection for pressure support. Field data was previously released by Statoil and made available for a public benchmark study (NTNU Norway) testing history matching techniques including production and time-lapsed seismic data. We focus on well production data for history matching. MCMC is used for global parameter updates and uncertainty quantification in a Bayesian context. An implementation of an adjoint technique is applied for analytical sensitivity calculations and local parameter adjustments of rock properties. History matching results are presented for field wide and well-by-well production data. Consistency checks between updated and original geological model are presented for rock property distribution maps. Geostatistical measures including spatial correlations are used to quantify deviations between updated and original geological model. In conclusion scalability and performance efficiency of the practical workflow implementation is discussed with a perspective of a consistent feedback loop from history matching to geological modeling.
Treatment of Adamantinoma in Tibial Diaphysis by Hemicortical Resection and Strut Allograft

Introduction: Adamantinoma is a primary low-grade, malignant bone tumor, of unknown histogenesis which is a rare neoplasm, comprise only 0.1–0.5% of all primary bonetumors. It is slightly more common in men than women, with a ratio of 5:4. Wide tumour resection and limb salvage is the treatment of choice. Case report: A 24 year female reffered with chief complaints of pain and limping lasting for approximately 2 years. Plain X-ray showed an expansile multicystic lesion at the diaphysis of the left tibia anterior cortex with periosteal reaction . We used an oscillating bone saw to perform a total resection of the lesion preserving the anterior cortex. Tibial defect created with this resection was replaced with a 14 cm. long strut cadaveric femoral allograft that fixed to tibia with a dynamic commpresive plate (DCP) and screws. Discussion: In surgical treatment of Adamantinoma, total resection was not performed and remain intact posterior cortex to increased the patient satisfaction, at the same time, bone fixation and early weight bearing at the postoperative. We believe that the defect will be able to union with appropriate fixation of the allograft also be long size.
The ecophysiology of coralline algae in southern New Zealand

ii Acknowledgements . iv List of abbreviations . xi List of figures . xii List of tables . xvii Chapter 1 . 1 General introduction . 1 1.1. Coralline algae . 2 1.1.1. Taxonomy/diversity . 2 1.1.2. Ecological role . 5 1.1.3. Productivity . 6 1.2. Physiology of coralline algae . 6 1.2. 1. Nitrogen and phosphorus physiology . 6 1.2. 2. Photosynthetic physiology . 7 1.3. Environmental threats to corallines and ecosystem services . 12 1.3. 1. Light/sedimentation . 12 1.3. 2. Temperature . 12 1.3. 3. Nutrient pollution . 13 1.3. 4. Ocean acidification . 14 1.4. Coralline algae in New Zealand . 15 1.5. Thesis outline . 19 1.5. 1. Chapter 2: Environmental conditions in a shallow subtidal zone inhabited by coralline algae in a kelp forest . 19 1.5. 2. Chapter 3: Spatial and temporal variability in nutrient status and pigment concentration of coralline algae in a kelp forest . 20
Toxic Determinants of Staphylococcus aureus PSMα3 Cross-α Amyloid

Staphylococcus aureus acts as a commensal bacterium that can lead to life-threatening infections. Members of the phenol soluble modulins (PSMs) family secreted by this bacterium perform different activities involved in virulence; some are mediated by forming amyloid fibrils of diverse architectures. PSMα3 is the most cytotoxic member of the family and assembles into amyloid-like cross-α fibrils of tightly mated sheets in which α-helices replace the β-strands composing typical cross-β amyloid fibrils. Using an array of PSMα3 mutants and derivatives, we herein substantiate the critical roles of fibrillation and positive charges in PSMα3 toxicity, and suggest a model in which cross-α fibrillation regulates available positive charges via inter-helical electrostatic interactions. We further show that PSMα3 aggregation on cell membranes leads to protein-independent deformation and cell death. The results provide mechanistic insight into amyloid toxicity, as well as offer strategies to design agents against key virulence determinants.
Endemicity of drug resistant Plasmodium falciparum in a district of Maehongson province

1 Department of Biochemistry, Faculty of Medicine, Chiangmai University, Chiangmai Province 50200 2 National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology To monitor drug resistance level of parasites in the endemic areas, which is necessary for timely control of the parasites and effective use of antimalarial drugs, we collected blood samples from Plasmodium falciparum-infected malaria patients who lived in endemic area in Maesariang district, Maehongson province, Thailand (n = 36) during November 2011 to October 2013. The malaria samples were cultured and tested in vitro with anti-malarial drugs. The finding showed that these P. falciparum parasites were still resistance to PYR and CQ, even though PYR and CQ have not been used in the area for a long time.
Treatment of Diabetic Ulcer using Autologous Fibroblast-Hyaluronic Acid Complex

Purpose: To evaluate clinical efficacy and safety of hyaluronic acid based autologous dermal fibroblasts (Hyalograft 3D) in the treatment of diabetic foot ulcers. Methods: A total of 28 patients with diabetic ulcers were randomized to either the control group with nonadherent foam dressings(n=14) or the treatment group with autologous tissue-engineered grafts(n=14). Weekly assessment contained vital sign checks, ulcer size measurements, and wound photos. In the 12th week, percentages of complete wound healing and mean healing times were compared. Safety was also monitored by adverse events. Results: Complete wound healing was achieved in 84.6% of the treatment group and 23.1% of the control group(p
MODELAMIENTO DE LA CINÉTICA DE HIDRÓLISIS ENZIMÁTICA DE PROTEÍNAS DEL PLASMA BOVINO

A kinetic model was used to study the reaction rate of hydrolysis of bovine plasma proteins and alcalase 2.4 L, in a batch reactor. The influence of variables, such as the concentration of initial enzyme substrate and the degree of hydrolysis was studied, and kinetic parameters of the rate equation were determined by analyzing its relationship with the work variables. A zero-order kinetic model and enzyme deactivation by substrate was found, as well as the direct relationship between the fraction of enzyme-substrate and the rate of formation of hydrolysis products.
基于诊断离子策略的超高压液相色谱-线性离子阱-轨道离子阱质谱联用技术解析中药虎杖的化学成分

: A method for qualitative analysis of constituents in Polygonum cuspidatum by ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) has been established. The methanol extract of Polygonum cuspidatumrn was separated on a Waters UPLC C18 column using acetonitrile-water (containing formic acid) eluting system and detected by LTQ-Orbitrap hybrid mass spectrometer in negative mode. The targeted components were further fragmented in LTQ and high accuracy data were acquired by Orbitrap MS. The summarized fragmentation pathways of typical reference components and a diagnostic fragment ions-searching-based strategy were used for detection and identification of the main phenolic components in Polygonum cuspidatum. Other clues such as nitrogen rule, even electron rule, degree of unsaturation rule and isotopic peak data were included for the structural elucidation as well. The whole analytical procedure was within 10 min and more than 30 components were identified or tentatively identified. This method is helpful for further phytochemical research and quality control on Polygonum cuspidatum and related preparations.
THE EVALUATIONAL STUDY ON ACCURACY OF 24 h DIETARY RECALL METHOD ASSISTED WITH ILLUSTRATIVE FOOD PICTURES

Objective To evaluate the effectiveness of the newly developed food pictures in improving the accuracy of 24 h dietary recall survey.Method Forty inpatients were selected to study the accuracy of recall method in dietary survey with or without illustrative food pictares as compared with the weighing method.The data of food consumption and nutrients intakes were collected for comparison.Results Compared with weighing method,besides egg and beef,all the foods and nutrients were underestimated by 24 h dietary recall method without pictures.The data of fat,vitamin E,sodium,calcium and dietary fiber intakes showed certain degree of positive correlation with those obtained by weighing method,but not correlated in other nutrients and food intakes.The data of foods and nutrients intakes obtained by pictures-assisted 24 h dietary recall method were highly correlated with those obtained by weighing method.Conclusion The newly developed food pictures can improve the accuracy of 24 h dietary recall method.It is easy to carry and use,especially in field work and deserves wider application. [ACTA NUTRIMENTA SINICA,2012,34(6):558-562]
CF-ABLE-UK score: Modification and validation of a clinical prediction rule for prognosis in cystic fibrosis on data from UK CF registry

Clinical rules are created to predict “good” or “poor” prognosis (lung transplantation or death) in cystic fibrosis (CF). The recent CF-ABLE Score (McCarthy C. et al Chest 2013; 143:1358) is a new simple rule with 4 commonly measured components. Our aim was to validate externally the CF-ABLE Score performance in follow-up (cohort) data from the UK CF Registry. This was a prospective study using retrospectively collected anonymised CF patients9 data (2007-2012). The CF-ABLE-UK score at registration was computed as sum [0-7] of FEV1% [0-3.5], exacerbations [0-1.5] and age*BMI [0-2]. The only exception from the original CF-ABLE rule was a modification for exacerbations as calculated here upon the number of days the patient required intravenous antibiotics. Means (SDs) in 3915 patients with complete data were: age 27.9 (8.9) years; FEV1% predicted 67.9 (24.8); BMI 22.3 (8.8); exacerbation days 18.1 (28.8). “Poor”/”good” outcome ratio was 9/91%. The CF-ABLE-UK score showed very good discrimination (AUC ROC 0.80 95%CI 0.79–0.83); as calibration, the score under-predicted the “poor” outcome in all risk strata similarly to the results from registry-based Irish validation data. The CF-ABLE Score delivered very good results in new, external data. Our preliminary findings suggest that the modified CF-ABLE-UK Score can be widely applied to other patients and settings and, particularly, in UK.
1-烯丙基-3-甲基咪唑离子液体单体与丙烯腈共聚行为的研究

以1-烯丙基-3-甲基咪唑盐酸盐([AMIM]Cl)作为离子液体单体,偶氮二异丁腈作为引发剂,二甲基亚砜为溶剂,实现了与丙烯腈(AN)在不同反应条件下的自由基共聚合. 研究了AN/[AMIM]Cl二元共聚合的动力学,得到了相应的竞聚率数据(rAN=8.11,r[AMIM]Cl=0). 用1H-NMR、GPC、DSC、TGA等手段对共聚物进行了表征. 通过对AN/[AMIM]Cl二元共聚及AN/IA/[AMIM]Cl三元共聚的研究发现,[AMIM]Cl在与丙烯腈共聚时具有双重作用,一部分离子单体进入到共聚物的分子链中调节其亲水性及可纺性,剩下的大部分留在纺丝液中可显著降低纺丝液的黏度,从而使高分子量高固含量纺丝液的纺制成为可能,有望得到高性能的碳纤维原丝.
Acute Myeloid Leukemia with t(8;21)(q22;q22) (AML1/ETO) in a Patient with Marked Hypocellularity and Low Blasts Count.

According to the World Health Organization (WHO) classification system, cases with t(8;21)(q22;q22) should be diagnosed as acute myeloid leukemia (AML) even with a blast count of less than 20 percent in blood or bone marrow. It is an uncommon manifestation, moreover hypocellularity is rarely observed in this subtype of leukemia. Here, we report a case of t(8;21) in a patient with marked hypocellularity of less than 5 percent and a blast count of less than 20 percent. This patient responded relatively well to chemotherapy. An allogeneic bone marrow transplantation was performed with good engraftment. This case suggests that hypocellular AML with a t(8;21) has as good a prognosis as hypercellular AML with t(8;21).
Human Epididymis Protein 4 in Early Diagnosis of Endometrial Cancer: A Systematic Review

Objective To systematically review the value of human epididymis protein 4(HE4) in early diagnosis of endometrial cancer. Methods Databases including The Cochrane Library(Issue 1, 2013), PubMed, MEDLINE(Ovid), CNKI, CBM and WanFang Data were electronically searched for relevant studies on HE4 versus the golden standard(pathological examination) in the diagnosis of endometrial cancer from inception to April 2013. Meanwhile, relevant journals were also manually searched. Two reviewers independently screened literature according to the inclusion and exclusion criteria, and evaluated the included studies using the QUADAS items. Then, meta-analysis was performed using RevMan 5.1 and Meta-DiSc 1.0. Results Finally, a total of 16 studies involving 2 299 women(1 088 endometrial cancer patients diagnosed according to the golden standard, of which, 504 with benign uterine disease and 707 with normal cervical) were included. The results of meta-analysis showed that, as for HE4 in early diagnosis of endometrial cancer(SEN=57%, 95%CI 0.54 to 0.60; SPE=92%, 95%CI 0.91 to 0.94; +LR=6.92, 95%CI 5.00 to 9.58; –LR=0.46, 95%CI 0.39 to 0.55; DOR=18.38, 95%CI 12.21 to 27.69; AUC=0.8817). Conclusion The current study indicates that serum HE4 is more sensitive and low specii c when applied in patients witThendometrial cancer, which is worth of being used in clinic. Due to the limitation of low quality of the included studies, more high quality trials are required to verify the above conclusion.
Application of Child Depression Inventory Among 9258 Primary and Secondary School Students

Objective To determine the prevalence and epidemiological features of depressive symptoms by using Child Depression Inventory(CDI) among primary and secondary school students,and to provide evidence for prevention measures against children' s depression.Methods By stratified-cluster sampling method,9 258 primary and secondary school students were collected to complete CDI.Results The total prevalence of depression symptom was 14.3%. The prevalence of boys was higher than girls in depression symptoms,especially among primary school students in urban areas.The distribution of the CDI mean score for all ages presented figure of "U",11-year-old group with the lowest mean scores of CDI and 18-year-old group with the highest.The prevalence of depression symptoms had great significant difference between primary and middle school students in urban areas,the prevalence was highest in students of senior high school and lowest in students of primary school.Conclusion Improvement in mental health status of school students depends upon reinforcement of mental health education and promotion.