Miracle-dz/UniFAR-FTUs huggingface dataset:
Electrophoresis‐assisted open‐tubular liquid chromatography/mass spectrometry for the analysis of lipooligosaccharide expressed by Campylobacter jejuni

Lipooligosaccharide (LOS) is the major component of the external membrane of Campylobacter jejuni. LOS contains a hydrophobic moiety, lipid A, and a hydrophilic moiety, oligosaccharide. Due to the unique mimicry of human ganglioside structures and potential involvement in the induction of the autoimmune polyneuropathies, Guillain–Barré and Miller Fisher syndromes, the structural characterization of C. jejuni LOS has received much attention. We have been using capillary zone electrophoresis–mass spectrometry to analyze O‐deacylated LOS from C. jejuni. In an attempt to optimize the separation conditions, the effect of methanol on the separation of LOS was investigated. It was found that methanol resulted in stronger adsorption of LOS onto the wall of fused‐silica capillary. In this paper, we applied this adsorption to perform electrophoresis‐assisted open‐tubular liquid chromatography electrospray mass spectrometry for the analysis of O‐deacylated LOS mixtures from C. jejuni. The analytical potential of the proposed strategy for the analysis of O‐deacylated LOS glycoforms from five bacterial colonies is demonstrated. Online tandem mass spectrometry is shown to provide a powerful tool for characterization of variations in the hexosamine backbone, phosphorylation of the lipid A, and sialic acid sequence information.
Application of Chronic Intravascular Blood Glucose Sensor in Dogs

An intravenous glucose sensor was implanted in six dogs for 1–15 wk. The glucose sensor is a flexible cylinder, ∼0.2 cm diam and 30 cm long, with a tip containing immobilized glucose oxidase and catalase coupled to a potentiostatic O2 sensor. The sensor and a similar O2 reference sensor were implanted in the superior vena cava near the entrance of the right atrium. The sensor response was conveyed externally either by a telemetry system implanted nearby, surgically accessed leads, or chronically maintained percutaneous leads. Summing over the six implants, there was a total implantation period of 333 days during which glucose sensors were functional on demand. The sensor response showed agreement with conventionally assayed blood samples after accounting for a response lag. Sensor response to glucose showed little change over the implant period. Biocompatibility, enzyme lifetime, O2 availability, O2 sensor stability, and biochemical interference were not limitations. Results demonstrated that this sensor can function effectively as an implant in dogs for a period of months and has the potential for long-term operation.
Establishment of Germline Competent C 57 BL / 6 J Embryonic Stem Cell Lines

Objective To establish C57BL/6J embryonic stem (ES) cell lines with potential germline contribution Methods ES cells were isolated from blastocyst inner cell mass of C57BL/6J mice, and cultured for 15 passages, and then injected into blastococels of ICR mice blastocysts to establish chimeric mice. Results Three ES cell lines (mC57ES1,mC57ES3, mC57ES7) derived from the inner cell mass of C57BL/6J mice blastocysts were established. They were characteristic of undifferentiated state, including normal XY karyotype, expression of a specific cell surface marker “stage-specific embryonic antigen-1” and alkaline phosphatase in continuous passage. When injected into immunodeficient mice, mC57ES1 cells consistently differentiated into derivatives of all three embryonic germ layers. When mC57ES1 cells were transferred into ICR mice blastocysts, 4 chimeric mice have been obtained. One male of them revealed successful germ-line transmission. Conclussion We have obtained C57BL/6J ES cell lines with a potential germ-line contribution, which can be used to generate transgenic and gene knock-out mice.
$\mu$ Approach to Robust Stability Domains in the Space of Parametric Uncertainties for a Power System With Ideal CPL

Power electronic systems are prone to instability. The problem, generally attributed to the constant power load (CPL) behavior of their power electronic controlled loads, can become more acute when the systems are subject to parametric uncertainties. The structured singular value (SSV) based <inline-formula><tex-math notation="LaTeX">$\mu$ </tex-math></inline-formula> method has proven to be a reliable approach for assessing the stability robustness of such uncertain systems. Despite its numerous benefits, the <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math> </inline-formula> method is not often applied to electrical power systems (EPS) with multiple uncertainties. This may be due to the mathematical complexity underlying the <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math> </inline-formula> theory. This paper aims to make the <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math> </inline-formula> approach more application-friendly by providing clearer insights into the meaning and usefulness of the robust stability measure <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula> for EPS with multiple parametric uncertainties. This is achieved by presenting a methodology for translating <inline-formula> <tex-math notation="LaTeX">$\mu$</tex-math></inline-formula> analysis results from the frequency domain to the more perceivable uncertain parameters domain. The method directly demonstrates dependences of system stability on uncertain system parameters. Further, it clearly identifies robust stability domains as subsets of the much wider stability domains. The work is based on a representative EPS connected to an ideal CPL. <inline-formula> <tex-math notation="LaTeX">$\mu$</tex-math></inline-formula> analysis predictions are evaluated and validated against analytical results, for example, CPL system.
COMMIT at SemEval-2017 Task 5: Ontology-based Method for Sentiment Analysis of Financial Headlines

This paper describes our submission to Task 5 of SemEval 2017, Fine-Grained Sentiment Analysis on Financial Microblogs and News, where we limit ourselves to performing sentiment analysis on news headlines only (track 2). The approach presented in this paper uses a Support Vector Machine to do the required regression, and besides unigrams and a sentiment tool, we use various ontology-based features. To this end we created a domain ontology that models various concepts from the financial domain. This allows us to model the sentiment of actions depending on which entity they are affecting (e.g., ‘decreasing debt’ is positive, but ‘decreasing profit’ is negative). The presented approach yielded a cosine distance of 0.6810 on the official test data, resulting in the 12th position.
Effects of age and obesity on insulin binding to isolated adipocytes.

UNLABELLED We have measured insulin binding to isolated adipocytes prepared from rats of varying ages and body weights. The ability of adipocytes to bind insulin progressively decreases as animals get older and fatter until about 70 days of age and 300 g body weight are reached. From this point on further decreases in insulin binding to adipocytes were not seen as rats got older and fatter. Analysis of the data indicated that this decrease in insulin binding could be accounted for by decreased numbers of insulin receptor sites per cell. Further studies were conducted in which animals were allowed to age, but obesity was prevented or reversed by hypocaloric diets. In these experiments decreased insulin binding was either prevented or restored to normal by the negative caloric state, indicating that age had no appreciable effect on the ability of adipocytes to bind insulin. The influence of the obesity associated variables-hyperinsulinemia and increased fat cell size-on insulin binding was also examined. These latter studies are consistent with the concept that elevated in vivo plasma insulin levels lead to decreased insulin receptors, and further suggest a role for additonal factors in regulating the adipocyte insulin receptor. IN CONCLUSION 1. Decreased insulin binding to adipocytes is closely related to the obese state. 2. This decrease in insulin binding can be accounted for by decreased numbers of receptor sites per cell, and 3. The mecahnism(s) underlying this decreased insulin binding are complex and probably represents an effect of more than one variable.
Matthias Kranz ( Editors ) Touching the Void-Haptic Feedback & Brain Computer Interfaces Advances in Embedded

This paper introduce the idea and necessity of using AirFlow for Haptic Feedback. 3 Different approaches for using the air-flow as a medium to getting haptic feedback in virtual world will be discussed. At first there is an introduction to this rather new technology and the importance of having such feedback, then we move to example parts which we study 3 different technologies in this field based on multiple different papers published by other researchers. Each technology will be discussed in details with possible applications and also the limitations of them, at the end we conclude the whole idea of using the air-flow for haptic feedback in virtual world and a short anticipation of future of this technology.
Safety evaluation of olive phenolic compounds as natural antioxidants

Free and total polyphenolic compounds were extracted from the fruits and leaves of the Picual cultivar. The safety limits of these compounds were recognized by measuring the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the levels of high-density lipoprotein (HDL) cholesterol and total lipids of rat serum. The free and total phenolic compounds (400, 800, and 1600 ppm) and butylated hydroxy toluene (BHT) (200 ppm) were daily ingested for 7 weeks. The administration of olive total and free phenolic compounds at 400 and 800 ppm did not cause any significant changes on ALT and AST activities and serum total lipids. These compounds at 1600 ppm caused significant increase in ALT and AST activities and the content of total lipids. Both olive phenolic compounds were superior to that of BHT in increasing HDL-cholesterol level. Nutritional experiments demonstrated that BHT at 200 ppm caused an enlargement in the kidney and liver of the rat compared with the administration of total and free olive phenolic compounds at 1200 and 1600 ppm. Microscopical examination of kidney and liver tissues of rats administered free and total phenolic compounds at 1200 ppm had the same histological character as that of control rats, while the administration of BHT (200 ppm) and phenolic compounds (1600 ppm) induced severe damage to the tissues of the rat kidney and liver.
Phase change memory as synapse for ultra-dense neuromorphic systems: Application to complex visual pattern extraction

We demonstrate a unique energy efficient methodology to use Phase Change Memory (PCM) as synapse in ultra-dense large scale neuromorphic systems. PCM devices with different chalcogenide materials were characterized to demonstrate synaptic behavior. Multi-physical simulations were used to interpret the results. We propose special circuit architecture (“the 2-PCM synapse”), read, write, and reset programming schemes suitable for the use of PCM in neural networks. A versatile behavioral model of PCM which can be used for simulating large scale neural systems is introduced. First demonstration of complex visual pattern extraction from real world data using PCM synapses in a 2-layer spiking neural network (SNN) is shown. System power analysis for different scaled PCM technologies is also provided.
Inverse correlation between Ink4‐locus deletions and ICM‐DNA hyperdiploidyin childhood acute lymphoblasticleukaemia, relation to clinicalcharacteristics and outcome

Abstract: Malignant cells from 72 children with ALL were analysed to investigate the relationship between DNA‐ploidy and deletion of the Ink4 locus containing the cell‐cycle regulating genes p16ink4a, p15ink4b and p14ARF. Image‐DNA cytometry (ICM) was used to assess DNA index (DI), and Southern hybridisation was carried out to detect deletions of the Ink4‐locus in the leukaemic cells. A DNA content equal to or exceeding 1.16, indicating hyperdiploidy, was detected in 21/72 patients (29%), 1/72 (1.3%) showed DNA‐hypodiploidy, and the remaining 50 patients (69%) had a DI within normal limits. Bi‐allelic deletion of at least two of the coding sequences from the Ink4 locus was observed in 23/70 (33%) patients. Mono‐allelic deletions within the locus were observed in 10/70 patients (14%), and 37/70 patients (53%) had normal signals for both sequences. Out of the 70 patients that could be analysed by both techniques only two had the combination of DNA hyperdiploidy and Ink4‐locus bi‐allelic deletion (p=0.004). DNA hyperdiploidy was not associated with any specific clinical characteristics, but there was a trend for a better prognosis for patients with DNA hyperdiploidy (p=0.09). Ink4‐locus deletion was associated with T‐cell phenotype and higher white blood cell counts at diagnosis and poor prognosis (p=0.0015). Multivariate analysis confirmed that Ink4‐locus deletion is an independent prognostic marker and a stronger determinant of outcome than DNA ploidy. When DNA ploidy and Ink4‐locus deletions were combined, novel subgroups with significantly different outcome could be observed. A group with DNA index 1.16 and no Ink4‐locus bi‐allelic deletions had an excellent prognosis (p‐EFS 0.93 at 60 months), patients with Ink4‐locus bi‐allelic deletion and a DNA index <1.16 fared worst (p‐EFS 0.57) and patients with no Ink4 deletions and without hyperdiploidy had an intermediate outcome (p‐EFS 0.79). The reason for the inverse correlation between DNA ploidy and Ink4 deletion and their combined impact on prognosis remains unclear, and possible reasons are discussed.
Modern metal-on-metal hip resurfacing: important observations from the first ten years.

Total hip resurfacing, in which a cementless acetabular component is combined with a metal-on-metal (cobalt-chromium alloy) bearing, was introduced approximately ten years ago. Previous resurfacing devices had failed because of insufficient initial fixation or as a result of progressive inflammatory bone resorption (osteolysis) due to high volumetric wear of the large-diameter acetabular bearing, which is composed of conventional polyethylene1,2. Extensive pelvic bone loss often made for a challenging revision. Current-generation cementless monoblock metal-on-metal bearing acetabular components are thin shelled (<5 mm). The amount of acetabular bone removed is no greater than that which would be removed during standard total hip arthroplasty. The cementless fixation principles that apply to the placement of these components are the same as those established over the past twenty years for the placement of standard total hip prostheses. Extensive wear simulation testing by numerous investigators has proven that the combination of high carbon content, low diametrical clearance, and low surface roughness produces the lowest wear of metal-on-metal bearings3-6. Improvements in manufacturing allow the current generation of these bearings to be more consistently machined to tight tolerances. Given that the alloy has a high carbon content and the manufacturing parameters are held constant, heat-treating should have no appreciable effect on wear resistance7. The clinical results from the first ten years come primarily from England, Australia, and Western Europe. Due to U.S. Food and Drug Administration (FDA) regulations, the U.S. experience is comparatively limited, although the devices from several American orthopaedic manufacturing companies are available in the non-U.S. market. Currently, there are two devices that have full FDA approval and several others that are still in the clinical-trial stage and awaiting approval. The following is an overview of modern metal-on-metal total hip resurfacing, highlighting the lessons learned over …
[Health literacy and its relative factors to residents in three cities in China].

OBJECTIVE To measure, evaluate health literacy and discover its relative factors among residents of three cities in China. METHODS Multiple cluster sampling was employed and 3300 respondents were surveyed by self-designed questionnaires in Beijing, Datong and Shenzhen city during May to September in 2011. Information on demographic characteristics, health knowledge and health literacy was collected. Respondents' health literacy scores were statistically reported and evaluated referring to education level. To explore relative factors of health literacy, multiple linear regression model with score of health literacy as dependent variable, respondents' demographic characteristics and health knowledge as independent variables was built by multiple linear regression analysis. RESULTS Questionnaires were conducted among 3300 residents and resulted in 90.9% (3000/3300) qualified sample return. Respondents were (31.6 ± 12.0) (15 - 65) years old, who got (19.92 ± 5.17) (2 - 28) scores in the health literacy test with an average correct rate of 71.1%. The proportion of subjects with low (< 20.5 grades), medium (20.5 - 24.5 grades), and high (> 24.5 grades) level of health literacy were 46.6% (1398/3000), 33.1% (993/3000) and 20.3% (609/3000) respectively. The multiple linear regression model showed that positive correlation factors of health literacy included health knowledge (β = 0.28), education level (β = 0.28), income (β = 0.14), gender (β = 0.05), nationality (β = 0.05), registered permanent residence (β = 0.05) (all P values < 0.05) and the negative correlated factors included age (β = -0.28), occupation (β = -0.05), respectively (all P values < 0.05). CONCLUSION Over 50% residents in the three studied cities had medium and above health literacy. The positive correlated factors of health literacy included health knowledge, education level, income, gender, nationality, registered permanent residence and the negative correlated factors included age and occupation.
Source Address Validation Improvement (SAVI) Framework

Source Address Validation Improvement (SAVI) methods were developed to prevent nodes attached to the same IP link from spoofing each other's IP addresses, so as to complement ingress filtering with finer-grained, standardized IP source address validation. This document is a framework document that describes and motivates the design of the SAVI methods. Particular SAVI methods are described in other documents.
Types of Pacemakers and their Complications

are: 1. Implantable pulse generators with endocardial or myocardial electrodes for long-term or permanent use. 2. External, miniaturized, transistorized, patientportable, battery-powered, pulse generators with exteriorized electrodes for temporary transvenous endocardial or transthoracic myocardial pacing. 3. Console batteryor AC-powered cardiovertors, defibrillators, or monitors with high-current external transcutaneous or low-current endocardial or myocardial circuits for temporary pacing in asynchro-
Business Process Redesign: Tactics for Managing Radical Change

By definition, business process redesign (BPR) represents radical change in today's bureaucratic functionally structured and managed organizations. The radical change theorists predict that to accomplish radical change requires the use of revolutionary change tactics. We propose that as the "radicalness" of the planned change increases, more revolutionary change tactics are used. We analyze the change tactics of three organizations' BPR initiatives to understand whether and how revolutionary tactics were used. The initiatives evinced a varied amount of revolutionary tactics depending on the scope and depth of planned change. The use of revolutionary tactics also varied by the phase of the initiatives. The frequency of revolutionary tactics was highest in the early phases of the initiatives and decreased as they approached implementation. We explore the reasons for reduced deployment of revolutionary tactics. We conclude by implications to BPR practice and research.
Metabolic syndrome criteria: ready for clinical prime time or work in progress?

This editorial refers to ‘Impact of statins in microalbuminuric subjects with the metabolic syndrome: a substudy of the PREVEND Intervention Trial’† by C.A. Geluk et al. , on page 1314 Geluk et al. 1 describe a subgroup analysis of the PREVEND trial in which they tested whether pravastatin reduced risk for major adverse cardiac events in micro-albuminuric patients with and without the metabolic syndrome defined by the National Cholesterol Education Program (NCEP) Adult Treatment Panel (ATP III) criteria.2 Although pravastatin did not reduce incidence of adverse cardiac events in the total cohort,3 an impressive 60% relative risk reduction was noted in the subgroup with both micro-albuminuria and metabolic syndrome [hazard ratio (HR) 0.39, 95% confidence interval (CI) 0.17–0.89], whereas micro-albuminuric individuals without metabolic syndrome did not appear to benefit. The higher event rate in the former group concurs with other evidence indicating a more ‘malignant’ outcome when micro-albuminuria accompanies other risk factors (e.g. endothelial dysfunction4) than when it occurs in isolation. The investigators suggested that their study supports the use of statins in micro-albuminuric subjects with metabolic syndrome to reduce incidence of major coronary events. So what does this mean for clinicians in practice? Should we advocate that metabolic syndrome status be recorded in all micro-albuminuric patients, and that differential treatment with statins of micro-albuminuric patients with and without metabolic syndrome become routine practice? To address these issues, several aspects of the currently reported findings would benefit from further comment. First, the total number of cardiac events in this study numbered <50, relatively modest compared with major statin trials. Therefore, one explanation for lack of apparent statin benefit in subjects with micro-albuminuria but without … *Corresponding author. Tel: +44 141 211 4312; fax: +44 141 553 2558. E-mail address : nsattar{at}clinmed.gla. ac.uk