UV spectroscopic method for determination of phenytoin in bulk and injection forms

ISSN: 2410-9649 Gul and Hameed / Chemistry International 4(3) (2018) 177-182 iscientic.org. 178 www.bosaljournals/chemint/ editorci@bosaljournals.com well as control seizures. It acts by dropping the extent of seizure movement in the brain. Phenytoin works on sodium channels at neuronal cell membrane, regulating the extent of seizure commotion and plummeting seizure promulgation. Through endorsing sodium efflux commencing neurons, Phenytoin inclines to steady the threshold in contradiction of hyper impulsiveness instigated by extreme stimulation or ecological variations proficient of plummeting membrane sodium gradient. This embraces the lessening of post-tetanic potentiation on synapses, averts cortical seizure foci from exploding contiguous cortical areas (Shah et al., 2017). Phenytoin utilizes an advantageous consequence through reducing seizures simply for the duration of the first week subsequently severe head injury (Temkin et al., 1990). Magnesium sulfate is superior to Phenytoin for the preclusion of eclampsia in hypertensive pregnant women (Lucas et al., 1995). Steady-state serum-Phenytoin intensities are dignified to regulate the dosage to attain the appropriate therapeutic concentration (Richens, and Dunlop, 1975). Different Methods have been developed for HPLC and UV determination of Phenytoin sodium in bulk forms either alone or in combinations (Mulsa et al., 2012; Shah et al., 2017; Bagade et al., 2014; Prasad et al., 1997; Lensmeyer et al., 1997; Lu-Steffes et al., 1982; Dykeman and Ecobichon, 1979; Bhatti et al., 1998; Sawchuk and Cartier, 1980; Abbaspour and Mirzajani, 2005; Mei and Williams, 1997; Dean et al., 1983; Kishore et al., 2003; Santagati et al., 2005; Vedsö et al., 1969; Castro et al., 1978; Löscher and Göbel, 1978; Cwik et al., 1997). But no UV method was available for the determination of Phenytoin sodium in Injection form. Our aim was to develop a simple, inexpensive and rapid method for the determination of Phenytoin sodium in both bulk and injection forms. The present research work describes the estimation of assay content of Phenytoin in active pharmaceutical ingredient (API) and ampoule dosage form using ultraviolet-Visible (UV-Vis) spectrophotometry technique. The work provides a sensitive, specific, as well as economical method for the determination of Phenytoin in very short time by the UV-Vis spectrophotometer. Distilled water is used as a diluent established on the drug solubility properties. Developed UV-Vis spectrophotometric method was validated with respect to Official guidelines. MATERIAL AND METHODS Chemical, reagents and instruments Phenytoin, distilled water. Glass wares of pyrex material were incorporated. Weighing balance ‘Shimadzu Japan’ and Spectrophotometer (UV-1601), UV/Visible Fig. 1: Chemical Structure of Phenytoin Fig. 2: Calibration curve for Phenytoin y = 0.1162x 0.002 R2 = 0.9998 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 0 2 4 6 8 10 A b so rb an ce Concentration (μg/mL) Absorbance 202nm Table 1: Calibration data of phenytoin for absorbance maxima Concentration (μg/mL) Absorbance 2 0.227 4 0.469 5 0.577 6 0.696 8 0.926 Table 2: Parameters from the calibration curve Parameters Observation at 202 nm Calibration Curve Linear