Miracle-dz/UniFAR-FTUs huggingface dataset:
Real time sequence characterized amplified region (RT- SCAR) marker: Development and its application for authentication and quantification of Catharanthus roseus L. Don.

The objective of the present study was to develop a Real Time-Sequence Characterized Amplified Region (RT-SCAR) of Catharanthus roseus, an effective molecular marker for authentication and quantification. Here, we made a comparative study of SCAR and RT-SCAR marker. These newer RT-SCAR methods are useful for the correct identification and quantification of C. roseus. In particular, this technique is very attractive and can be used as a novel tool for quantification of C. roseus when the SCAR results cannot be quantified and the RAPD results are not consistent. RAPD analysis of collected samples from different geographical locations in India was carried out with 25 random primers. The RAPD polymorphic band was cloned, sequenced and from the sequence information, primers pair for SCAR was developed. The same primers was used for RT-SCAR marker by using SYBR green fluorescence-based detection in Real Time PCR. Utilizing this newly specific RT-SCAR marker, we detected the presence and quantity of C. roseus in all the analyzed several samples, by aiding 10% its substitute and adulterants at time of DNA isolation. No difference was found in pure sample and 10% adulterant sample with the help of SCAR but in the case of RT-SCAR difference was found in the DNA sample. This analytical strategy could be used further for large scale quantification of C. roseus’s market samples. Key words: Catharanthus roseus, RAPD, Sequence-Characterized-Amplified-Region (SCAR) Marker, Real Time Sequence-Characterized-Amplified-Region (RT-SCAR) Marker, Real Time PCR.
Dynamics of a Strongly Nonlinear Spacecraft Structure Part II: Modal Analysis

The present paper investigates the dynamics of a real-life spacecraft structure possessing a strongly nonlinear component with multiple mechanical stops. A full-scale finite element model is built for gaining additional insight into the nonlinear dynamics that was observed experimentally in a companion paper [NRK14]. To this end, advanced techniques and theoretical concepts such as numerical continuation and nonlinear normal modes are exploited.
A genotyping assay for missense mutation in WISP 3 gene associated with childhood onset pseudorheumatoid arthropathy

Introduction: Progressive pseudorheumatoid dysplasia (PPD) is an autosomal recessive genetic disorder reported to be caused by gene alterations of the Wnt1-inducible signaling pathway protein 3 corresponding gene (WISP3) located on chromosome position 6q22. Up to date, there is only a handful of WISP3 mutations identified in Europe, whereas most mutations are identified in Asia and Middle East. According to our knowledge, this is the first report of genetic dissection of WISP3 associated with spondyloepiphyseal dysplasia tarda from Bosnia and Herzegovina. Based on clinical examination findings (general manifestations, physical examination, characteristics of their bones on X-ray and laboratory results), an index patient was directed to WISP3 genotyping for confirmation of suspected diagnosis of PPD. Methods: DNA was extracted from peripheral blood leukocytes. All 5 exons and their exon-intron boundaries of the WISP3 gene were amplified by polymerase chain reaction (PCR) and sequenced by Sanger method. Segregation analysis was done to confirm the familial carrier status. Results: A missense mutation (C223G) homozygous T to G transition at c.667 in exon 4 was identified in index patient. This mutation changed codon CAG to TAG and resulted in a subsequent change of the cysteine to glycine codon. Same mutation was observed in both parents in heterozygous form confirming the familial segregation. Conclusion: Due to its nature, the identified mutation C223G in exon 4 in WISP3 gene is the most probably causative for PPD in described patient. Here we describe the PCR based method for genotyping of specific mutation in WISP3 gene. The identification of this mutation might be a valuable addition to a regional databases on rare genetic variant although a functional analysis should be performed to explain its pathological effect.
A Black–Litterman approach to correlation stress testing

Correlation stress testing is motivated by a well-known phenomenon: correlations change under financial crises. The adjustment of correlation matrices may be required to evaluate the potential impact of these changes. Very often, some correlations are explicitly adjusted (core correlations), with the remainder left unspecified (peripheral correlations), although it would be more natural for both core correlations and peripheral correlations to vary. However, most existing methods ignore the potential change in peripheral correlations. In this paper, we propose a Black–Litterman approach to correlation stress testing in which the stress impact on the core correlations is transmitted to the peripheral correlations through the dependence structure of the empirical correlations.
Pose with Uncertainty Single RGB Input Image Stochastic Pose Samples from Bayesian Convolutional Network

We present a robust and real-time monocular six degree of freedom visual relocalization system. We use a Bayesian convolutional neural network to regress the 6-DOF camera pose from a single RGB image. It is trained in an end-to-end manner with no need of additional engineering or graph optimisation. The algorithm can operate indoors and outdoors in real time, taking under 50ms to compute. It obtains approximately 2m and 3◦accuracy for very large scale outdoor scenes and 0.5m and 5◦accuracy indoors. Using a Bayesian convolutional neural network implementation we obtain an estimate of the model’s relocalization uncertainty and obtain a 10 15% improvement in state of the art localization accuracy on a large scale outdoor dataset. We leverage the uncertainty measure to estimate metric relocalization error and to detect the presence or absence of the scene in the input image. We show that the model’s uncertainty is caused by images being dissimilar to the training dataset in either pose or appearance.
Accurate and Detailed Transversal Road Section Characteristics Extraction Using Laser Scanner

: Road vehicle lateral positioning is a key aspect of many assistance applications and autonomous driving. However, conventional GNSS-based positioning systems and fusion with inertial systems are not able to achieve these levels of accuracy under real trafﬁc conditions. Onboard perception systems provide knowledge of the surroundings of the vehicle, and some algorithms have been proposed to detect road boundaries and lane lines. This information could be used to locate the vehicle in the lane. However, most proposed algorithms are quite partial and do not take advantage of a complete knowledge of the road section. This paper proposes an integrated approach to the two tasks that provides a higher level of robustness of results: road boundaries detection and lane lines detection. Furthermore, the algorithm is not restricted to certain scenarios such as the detection of curbs; it could be also used in off-road tracks. The functions have been tested in real environments and their capabilities for autonomous driving have been veriﬁed. The algorithm is ready to be merged with digital map information; this development would improve results accuracy.
Detecting Aerosols over Land from Satellites by Measuring Far IR Radiation from the Earth-Atmospheric System

Abstract In this study, a special method is proposed for detecting aerosols over land by analyzing satellitemeasured far IR radiation at three channels, 8.7, 11.5, and 12. 5µm. Sensitivity tests revealed that the behavioral features of radiative brightness temperatures (BTs) at these three channels with increasing optical depth are different among different types of aerosols. Analyzing how BTs and their differences, xBT (BT11.5-BT12.5) and yBT (BT8.7-BT11.5), behave with varying optical depth can help to qualitatively distinguish among aerosols and semi-quantitatively estimate their optical thicknesses. In addition, the authors found that the vertical density profile of aerosols has little impact on this method.
Because of the usually late stage of diagnosis , the trajectory of treatment among ovarian cancer survivors has numerous physical , psychological , and social effects

91 n the United States, it is estimated that more 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 I than 230,000 women are living as ovarian cancer survivors (Siegel, Miller, & Jemal, 2016). Cancer survivorship, as defined by the National Cancer Institute (2016), begins after the time of diagnosis and extends until the woman’s death, regardless of cause of death. Ovarian cancer occurs without early signs or symptoms, and up to 77% of women with ovarian cancer have advanced disease at diagnosis with poor prognosis (Lockwood-Rayermann, 2006; Price et al., 2016). The clinical course for women with advanced disease often involves aggressive therapies, including surgery and multiple courses of chemotherapy that usually include six cycles of combination chemotherapy with cisplatin or carboplatin plus a taxane (Lockwood-Rayermann, 2006). Approximately 80% of women experience disease recurrence and subsequently require additional courses of chemotherapy and adjuvant modalities over a number of years (Beesley et al., 2013). Because of the usually late stage of
Influence of culture medium and age of zygotic embryos on in vitro germination of Elaeis guineensis Jacq.

Oil palm cultivation has acquired economic importance due to rising demand for vegetable oils used in food, pharmaceutical, cosmetic and most recently, in biofuel industries. However, the commercial production increase of oil palm is limited by plantlet production, as it primarily uses seeds that require a long germination period and have low germination rates. In this study we evaluated the effect of embryo age (time after pollination), culture media and presence of carbohydrates on in vitro germination of zygotic embryos of oil palm (Elaeis guineensis Jacq.) Manicore hybrid. Embryos at 80, 90, 100 and 110 days post-anthesis were inoculated in MS medium or modified Y3 medium, with or without 3% sucrose. Media were renewed after 30 days of inoculation, and plantlets were kept for 45 days until collection for histological analyses. The embryos did not germinate in medium without sucrose. Embryos of 100 days post-anthesis in MS medium showed the best result for germination rate (88%), which did not differ statistically from Y3 medium. As for the parameters, 90-day embryos showed better results for number and length of roots in Y3 medium. It was concluded that 90-day embryos cultivated in Y3 medium generate plantlets in better conditions to be transferred to acclimatization. Key words: Oil palm (Elaeis guineensis Jacq.), germination, sucrose, Y3 medium, MS, anatomy, embryo culture.
Microplastics in the Marine Environment: Current Status, Assessment Methodologies, Impacts and Solutions

Microplastics exhibit a global distribution and have been detected in all levels of the marine environment. The most visible and disturbing impact of microplastics are their ingestion and consequent suffocation of hundreds of marine species. Microplastics can contribute considerably to the transport of non-indigenous marine species to a new area thereby threatening the marine biodiversity and the food web. They can also accumulate toxic substances on their surface therefore represent a potential concentrated source of environmental pollution or act as a vector of toxic pollutants in the food web with some severe health implications. Therefore, assessment of the status, effect and mitigations are needed. The current status, effects and solutions to the occurrence of microplastics in the marine environments were conducted by reviewing most of the literatures on the subject matter. Here, we particularly discussed old and new techniques for extracting and quantifying microplastics from marine matrices. Although, it is an intricate and herculean task to address the problems of microplastics in the ocean, some of the approaches to mitigate them are discussed.
Running title : CER 17 / ADS 4 involvement in wax and cutin production

Running title: CER17/ADS4 involvement in wax and cutin production 1 2 3 4 Shiyou Lü 5 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture 6 Wuhan Botanical Garden, Chinese Academy of Sciences 7 Wuhan 430074, China 8 Phone (Day): 86-27-87518809 9 Phone (Evening): 86-13871209035 10 Email: shiyoulu@wbgcas.cn 11 12 13 Plant Physiology Preview. Published on January 9, 2017, as DOI:10.1104/pp.16. 01956
Zadow, Corinne and Houghton, Stephen and Hunter, Simon C. and Rosenberg, Michael and Wood, Lisa (2017) Associations between positive mental well-being and depressive symptoms in Australian adolescents. The Educational and Developmental Psychologist,

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Is there really a debris disc around ζ2 Reticuli?

The presence of a debris disc around the Gyr-old solar-type star $\zeta^2\,\mathrm{Reticuli}$ was suggested by the $\mathit{Spitzer}$ infrared excess detection. Follow-up observations with $\mathit{Herschel}$/PACS revealed a double-lobed feature, that displayed asymmetries both in brightness and position. Therefore, the disc was thought to be edge-on and significantly eccentric. Here we present ALMA/ACA observations in Band 6 and 7 which unambiguously reveal that these lobes show no common proper motion with $\zeta^2\,\mathrm{Reticuli}$. In these observations, no flux has been detected around $\zeta^2\,\mathrm{Reticuli}$ that exceeds the $3\sigma$ levels. We conclude that surface brightness upper limits of a debris disc around $\zeta^2\,\mathrm{Reticuli}$ are $5.7\,\mathrm{\mu Jy/arcsec^2}$ at 1.3 mm, and $26\,\mathrm{\mu Jy/arcsec^2}$ at 870 microns. Our results overall demonstrate the capability of the ALMA/ACA to follow-up $\mathit{Herschel}$ observations of debris discs and clarify the effects of background confusion.
2 State of the Art in Entity Detection ( Spotting )

Named Entity Recognition (NER) and Named Entity Linking (NEL) are two research areas that have shown big advancements in recent years. The majority of this research is based on the English language. Hence, some of these improvements are language-dependent and do not necessarily lead to better resultswhen applied to other languages. Therefore, this paper discusses TOMO, an approach to language-aware named entity detection and evaluates it for the German language. This also required the development of a German gold standard dataset, which was based on the English dataset used by the OKE 2016 challenge. An evaluation of the named entity detection task using the web-based platform GERBIL was undertaken and results show that our approach produced higher F1 values than the other annotators did. This indicates that language-dependent features do improve the overall quality of the spotter.
Résumé Recherche sur les femmes soumises à une probation imposée par le tribunal ou à une ordonnance de probation

Le cadre de vie des délinquantes constitue un bon exemple des inégalités sanitaires que l’Organisation mondiale de la santé a cernées comme étant d’importants déterminants de la santé. Au cours de la dernière décennie, on a noté une hausse importante des condamnations menant à une détention correctionnelle chez les femmes, ce qui fait ressortir la nécessité pressante d’entreprendre des travaux de recherche dans ce domaine. Pour répondre à cette nécessité, une étude pilote a été menée au Canada, plus précisément en Ontario, en vue d’examiner les besoins des femmes qui ont des démêlés avec la justice en ce qui concerne la promotion et les notions en matière de santé. Les auteures font ressortir les aspects du processus de recherche employé auprès de délinquantes en énumérant les difficultés professionnelles et personnelles auxquelles peut se heurter l’équipe de recherche lors du déroulement d’une telle enquête. Dans leur conclusion, elles formulent des recommandations pour les futurs travaux de recherche entrepris auprès des délinquantes en se servant des leçons tirées de l’étude pilote.
Soy-isoflavone supplementation tends to reduce menopausal symptoms in postmenopausal women

BACKGROUND Menopausal symptoms in postmenopausal women tend to decrease healthrelated quality of life (HRQoL). The present study’s objective was to determine the effect of daily supplementation of 100 mg soy isoflavones on menopausal symptoms of healthy postmenopausal women. Isoflavones are phytoestrogens abundantly found in soy beans, and several studies have demonstrated that isoflavones are the best among the phytoestrogens. METHODS The study was a community-based double blind randomized controlled trial involving 60 healthy postmenopausal women, aged between 48–60 years, in the Mampang Prapatan District, South Jakarta. Participants were block-randomized to receive either 100 mg soy-isoflavone + 500 mg calcium carbonate (intervention group) or 500 mg calcium carbonate only (control group). Both supplements were taken daily for 12 weeks, from January to April 2010. Menopausal symptoms (measured by Menopause Rating Scale questionnaire) were assessed at baseline and after supplementation. Chi-square test was used to examine the effect of soy isoflavone supplementation on menopausal symptoms. RESULTS Fifty-six (93.3%) of participants completed the study. There were no statistically significant differences (p>0.05) in the prevalence of menopausal symptoms between the isoflavone group and the control group. However, supplementation of soy isoflavones for 12 weeks tended to decrease the prevalence of menopausal symptoms in women with normal body mass index and adequate daily dietary isoflavone intake. CONCLUSION Daily supplementation of 100 mg soy isoflavones for 12 weeks tend to decrease the incidence of menopausal symptoms in postmenopausal women.
The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage

Summary A method is proposed for estimating the percentage of dietary protein that is degraded by microbial action in the rumen when protein supplement is added to a specified ration. The potential degradability, p, is measured by incubating the supplement in artificial-fibre bags in the rumen and is related to incubation time, t, by the equation p = a+b(1 – e-ct). The rate constant k, measuring the passage of the supplement from the rumen to the abomasum, is obtained in a separate experiment in which the supplement is combined with a chromium marker which renders it completely indigestible. The effective percentage degradation, p, of the supplement, allowing for rate of passage, is shown to be p = a+[bc/(c+k)] (1-e-(e+k)t) by time, t, after feeding. As t increases, this tends to the asymptotic value a+bc/(c+k), which therefore provides an estimate of the degradability of the protein supplement under the specified feeding conditions. The method is illustrated by results obtained with soya-bean meal fed as a supplement to a dried-grass diet for sheep. The incubation measurements showed that 89% of the soya-bean protein disappeared within 24 h and indicated that it was all ultimately degradable with this diet. When the dried grass was given at a restricted level of feeding the allowance for time of retention in the rumen reduced the estimate of final degradability to 71% (69% within 24 h). With ad libitum feeding there was a faster rate of passage and the final degradability was estimated to be 66% (65% within 24 h).
Infection Treatment of Hepatitis C Virus Genotype 1 Variants for Prediction of Efficacy of Utility of Detection of Telaprevir-Resistant

Published Ahead of Print 6 November 2013. 10.1128/JCM.02371-13. 2014, 52(1):193. DOI: J. Clin. Microbiol. Hiromitsu Kumada Kobayashi, Satoshi Saitoh, Yasuji Arase, Kenji Ikeda and Hosaka, Masahiro Kobayashi, Tasuku Hara, Mariko Kawamura, Hitomi Sezaki, Yoshiyuki Suzuki, Tetsuya Norio Akuta, Fumitaka Suzuki, Taito Fukushima, Yusuke Infection Treatment of Hepatitis C Virus Genotype 1 Variants for Prediction of Efficacy of Utility of Detection of Telaprevir-Resistant
An all-optical velocity filter and beam splitter for generating cold molecular beams: a proposal and simulation

In order to generate one or two cold molecular beams that have neither a permanent electric dipole moment nor a magnetic dipole one, a controllable scheme to form an all-optical velocity filter and molecular beam splitter by using two red-detuned, crossing and cavity-enhanced guiding laser beams is proposed, and both the dynamic velocity filtering and beam splitting processes of the guided cold I2 molecular beam are studied by using the three-dimensional Monte Carlo method. Our study shows that by adjusting the laser power difference between the two guiding laser beams from −124 W to 124 W, a splitting ratio of the two-arm output molecular beams from about 10.3% to 89.7% can be obtained. Also, by adjusting the intersection angle between the two standing wave cavities from 80° to 10°, an adjustable splitting ratio from about 1.6% to 98.4% can be obtained. If the intersection angle between the guided oblique beam and the guided straight beam is set to 80°, a cold I2 molecular beam with a full-width at half-maximum longitudinal velocity of 1 m s−1 and a longitudinal temperature of 8 mK can be generated by using an all-optical velocity filter.
Chemical Composition and In Vitro Digestibility of Pleurotus ostreatus Spent Rice Straw

The nutritive value of rice straw (RS) and Pleurotus ostreatus spent rice straw (SRS) was studied by analyzing its proximate composition, fiber fractions, in vitro digestibility, amino acids content and scanning electron microscopy (SEM). The possibility of replacing berseem clover (BC; Trifolium alexandrinum) with SRS at different levels also was studied. Results showed higher protein content for SRS compared to RS (3.4 to 11.7%) while, DM, OM, NFE, CF, NDF, ADF, ADL, hemicellulose and cellulose were less for SRS than for RS. Highest concentration of amino acids (mg/100 g) was in SRS compared to RS. The SEM showed an extensive damage of SRS when compared to RS. Data also showed that SRS had higher in vitro dry matter disappearance (DMD) and in vitro organic matter disappearance (OMD) compared to RS. Results of in vitro study also, indicated that the levels of 50 and 90% replacement had the highest values of DMD and OMD compared to the other levels. It could be concluded from this study that treatment of RS with Pleurotus ostreatus improved the potential feeding value of the resultant substrates (i.e. SRS) as feed resources for ruminants and possibility of replacing BC with SRS at high levels of up to 50 or 90% from diets.
Recent Developments in Hamburg's Coastal Protection

In 2012 Hamburg’s government decided to raise the design water levels of the tidal River Elbe in the Hamburg region by about 0.80 m. As a consequence, the primary flood defences in the city have to be reinforced and heightened during the next 25-30 years. Prior to implementing the strengthening program for the dikes and sea walls, extensive design work has to be carried out, including wave simulations and the determination of freeboard heights, resulting in new crest levels for the various coastal flood protection facilities in the city.