Draft Genome Sequence of NYR20, a Red Pigment-Secreting Mutant of <named-content content-type='genus-species'>Saccharomyces cerevisiae</named-content>

A Saccharomyces cerevisiae mutant strain, NYR20, produces a red pigment owing to adenine auxotrophy. Unlike other yeast adenine biosynthetic mutants, this strain not only produces but also secretes this pigment. Here, we report the NYR20 draft genome sequence, thereby advancing our understanding of pigment secretion mechanisms. Adenine deficiency results in the accumulation of polyribosylaminoimidazole, a red pigment, in the vacuole of yeast adenine biosynthetic mutants ade1 and ade2 (1). A mutant strain of P-684 (a Saccharomyces cerevisiae strain isolated from the flowers of Prunus verecunda ‘Antiqua’ [2]), which produces and secretes a red pigment, was obtained and designated NYR20 (3). For this, we sporulated P-684 and obtained high ethanol-producing strains, which were then treated with ethyl methanesulfonate. Red colonies (exhibiting the adenine auxotrophic phenotype) were selected, and NYR20, a pigment-secreting strain, was isolated from the colonies. The Japanese refined sake prepared with NYR20 is red in color (3). Thus, to investigate the underlying mechanisms governing pigment secretion, we analyzed the genome sequence of NYR20. NYR20 cells were cultured in YPAD medium (1% yeast extract, 2% Bacto peptone, 2% glucose, 0.04% adenine sulfate) at 25°C. After collection, their genomic DNA was isolated using a Dr. GenTLE (from yeast) high-recovery kit (TaKaRa Bio, Inc.). A DNA library was prepared using a DNA library preparation kit (Beijing Genomics Institute, Shenzhen, China). DNA fragmentation was performed by using a g-TUBE (Covaris, Inc.) to produce fragments having an average length of 300 bp, followed by end repair, A tailing, adaptor ligation, and PCR; DNA library purification was performed according to the manufacturer’s instructions. Paired-end sequencing on the DNBSEQ-G400 platform (MGI Tech, Shenzhen, China) generated 16,720,478 reads (150-bp reads). Adaptor sequences were removed and low-quality reads were trimmed from the obtained short reads using fastp version 0.19. 10 (4) with default parameters. De novo sequence assembly was performed using SPAdes version 3.14.0 (5) with the “merged” and “isolate” parameters enabled. A reference-guided scaffolding of the draft genome sequence was conducted using RaGOO version 1.1 (6), with default parameters and the S. cerevisiae Citation Takahashi H, Iwaguchi S-I, Kondo H, Motomura T, Murase M, Takahashi A, Fukuyoshi S, Machida C, Kanamasa S, Yamamoto S, Yoshizaki T. 2021. Draft genome sequence of NYR20, a red pigment-secreting mutant of Saccharomyces cerevisiae. Microbiol Resour Announc 10:e01161-20. https://doi.org/10 . 1128/MRA.01161-20. Editor Antonis Rokas, Vanderbilt University Copyright © 2021 Takahashi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Hiro Takahashi, takahasi@p.kanazawa-u.ac. jp, or Takayuki Yoshizaki, tyoshizaki@fukuyama-u.ac. jp. Received 9 October 2020 Accepted 18 November 2020 Published 7 January 2021 January 2021 Volume 10 Issue 1 e01161-20 mra.asm. org 1 GENOME SEQUENCES