Miracle-dz/UniFAR-FTUs huggingface dataset:
Significant aortic stenosis associated with poorer functional outcomes in patients with acute ischaemic stroke undergoing endovascular therapy

Background and aim Bi-directional feedback mechanisms exist between the heart and brain, which have been implicated in heart failure. We postulate that aortic stenosis may alter cerebral haemodynamics and influence functional outcomes after endovascular thrombectomy for acute ischaemic stroke. We compared clinical characteristics, echocardiographic profile and outcomes in patients with or without aortic stenosis that underwent endovascular thrombectomy for large vessel occlusion acute ischaemic stroke. Methods Consecutive acute ischaemic stroke patients with anterior and posterior circulation large vessel occlusion (internal carotid artery, middle cerebral artery and basilar artery) who underwent endovascular thrombectomy were studied. Patients were divided into those with significant aortic stenosis (aortic valve area <1.5 cm2) and without. Univariate and multivariate analyses were employed to compare and determine predictors of functional outcomes measured by modified Rankin scale at three months. Results We identified 26 (8.5%) patients with significant aortic stenosis. These patients were older (median age 76 (interquartile range 68–84) vs. 67 (interquartile range 56–75) years, p = 0.001), but similar in terms of medical comorbidities and echocardiographic profile. Rates of successful recanalisation (73.1% vs. 78.0%), symptomatic intracranial haemorrhage (7.7% and 7.9%) and mortality (11.5% vs. 12.6%) were similar. Significant aortic stenosis was independently associated with poorer functional outcome (modified Rankin scale >2) at three months (adjusted odds ratio 2.7, 95% confidence interval 1.1–7.5, p = 0.048), after adjusting for age, door-to-puncture times, stroke severity and rates of successful recanalisation. Conclusion In acute ischaemic stroke patients managed with endovascular thrombectomy, significant aortic stenosis is associated with poor functional outcome despite comparable recanalisation rates. Larger cohort studies are needed to explore this relationship further.
New Approaches for Cryptococcosis Treatment

Cryptococcosis is an important opportunistic infection and a leading cause of meningitis in patients with HIV infection. The antifungal pharmacological treatment is limited to amphotericin B, fluconazole and 5- flucytosine. In addition to the limited pharmacological options, the high toxicity, increased resistance rate and difficulty of the currently available antifungal molecules to cross the blood–brain barrier hamper the treatment. Thus, the search for new alternatives for the treatment of cryptococcal meningitis is extremely necessary. In this review, we describe the therapeutic strategies currently available, discuss new molecules with antifungal potential in different phases of clinical trials and in advanced pre-clinical phase, and examine drug nanocarriers to improve delivery to the central nervous system.
A New Window onto the Pacemaker of the Heart, the Sinus Node, Provided by Quantitative Proteomics and Single-Nucleus Transcriptomics

Hypothesis-driven research has dominated biomedical science for at least the past century [1]. There are many papers and grant applications that will have been rejected because they are not hypothesis-driven. For example, Haufe [2] reports that the NIH guidelines for RO1 grants states that “A strong grant application is driven by a strong, solid hypothesis with clear research objectives”. More than 50 years ago, the Austrian-born British philosopher Karl Popper formalised this [3]. He believed that good science involves rigorously testing a hypothesis to determine its validity; when the hypothesis fails, a new hypothesis is formulated and after this the process is repeated [3]. The advantage of hypothesis-driven research is that it channels the investigator’s efforts, and it provides a framework for progressing our knowledge in a methodical stepwise manner. Undoubtedly, hypothesisdriven research is important and will continue to be so. However, hypothesis-driven research also blinkers the investigator to the complexity of nature and the temptation is for the investigator to attribute a phenomenon to one factor, which is rarely the case – nature is too complex. The alternative to hypothesis-driven research is hypothesis-generating or exploratory research and the advantage of this is that it does not constrain the view of the investigator. The amassing of enormous data sets in genomics, transcriptomics and proteomics as well as automated data-mining techniques have facilitated this alternative approach, resulting in ‘data-driven discovery’ [1].
The Design of a Frame-Like ZnO FBAR Sensor for Achieving Uniform Mass Sensitivity Distributions

In this paper, an infinite circular ZnO thin film bulk acoustic resonator (FBAR) with a frame-like electrode operating at the thickness-extensional (TE) mode is studied. Two-dimensional scalar differential equations established for the problem in the Cartesian coordinate system are successfully solved by transforming them into normal Bessel equations and modified Bessel equations in the cylindrical coordinate system. Resonant frequencies and vibration distributions are obtained for this frame-like FBAR sensor. A nearly uniform mass sensitivity distribution in the active area is achieved by designing proper electrode size and mass ratio of the driving electrode to the ZnO film. Numerical results show that compared with the reported ring electrode FBAR sensor, the novel frame-like electrode FBAR can achieve a maximum optimization ratio (up to 97.90%) on the uniformity of the mass sensitivity distribution in the active area under the same structural parameters, which is also higher than the optimization ratio 77.63% obtained by the reported double-ring electrode design. Moreover, the mechanism to achieve a very uniform mass sensitivity distribution in the active area by the frame-like electrode is explained in detail according to dispersion curves. Namely, when the resonant frequency of the FBAR sensor is close to the cut-off frequency of the active region in the dispersion curve, the mass sensitivity distribution is nearly uniform. These conclusions provide a theoretical guidance for the design and optimization of ZnO FBAR mass sensors with high performance.
A Vision-Based Method for Determining Aircraft State during Spin Recovery

This article proposes a vision-based method of determining in which of the three states, defined in the spin recovery process, is an aircraft. The correct identification of this state is necessary to make the right decisions during the spin recovery maneuver. The proposed solution employs a keypoints displacements analysis in consecutive frames taken from the on-board camera. The idea of voting on the temporary location of the rotation axis and dominant displacement direction was used. The decision about the state is made based on a proposed set of rules employing the histogram spread measure. To validate the method, experiments on flight simulator videos, recorded at varying altitudes and in different lighting, background, and visibility conditions, were carried out. For the selected conditions, the first flight tests were also performed. Qualitative and quantitative assessments were conducted using a multimedia data annotation tool and the Jaccard index, respectively. The proposed approach could be the basis for creating a solution supporting the pilot in the process of aircraft spin recovery and, in the future, the development of an autonomous method.
Potential of the natural products against leishmaniasis in Old World - a review of in-vitro studies

ABSTRACT Leishmaniasis is a vector-borne disease among the 10 most Neglected Tropical Diseases with diverse clinical manifestations caused by protozoan parasites of the Leishmania genus. Around 80% of leishmaniasis cases are found in the Old World affecting populations mainly in low and middle-income countries. Its control relies mostly on chemotherapy which still presents many drawbacks. Natural products may offer an inexhaustible source of chemical diversity with therapeutic potential. Despite the lack of knowledge on traditional products with activity against Leishmania parasites, many reports describe the search for natural extracts and compounds with antileishmanial properties against promastigote and amastigote parasite forms. This review summarizes the research of 74 publications of the last decade (2008–2018) focused on the identification of endemic plant-derived products that are active against Old World Leishmania parasites responsible for cutaneous and visceral leishmaniasis. The present review combines data on antileishmanial activity of 423 plants species, belonging to 94 different families, including a large range of crude extracts which lead to the isolation of 86 active compounds. Most studied plants came from Asia and most promising plant families for antileishmanial activity were Asteraceae and Lamiaceae. From the chemical point of view, terpenoids were the most frequently isolated natural products. These studies suggest that natural products isolated from Old World flora are a rich source of new chemical scaffolds for future leishmaniasis treatment as well as for other Neglected Tropical Diseases warranting further investigation.
Shared Decision‐Making Applied to Knee Arthroplasty: A Systematic Review of Randomized Trials

Shared decision‐making (SDM) is a strongly endorsed approach by which patients and clinicians work together to formulate a sensible care plan. The present study was undertaken to conduct a systematic review of SDM trials in patients considering knee arthroplasty (KA) to characterize how SDM was supported and the impact on care received.
Leukocyte Telomere Length Is Unrelated to Cognitive Performance Among Non-Demented and Demented Persons: An Examination of Long Life Family Study Participants

Abstract Objective: Leukocyte telomere length (LTL) is a widely hypothesized biomarker of biological aging. Persons with shorter LTL may have a greater likelihood of developing dementia. We investigate whether LTL is associated with cognitive function, differently for individuals without cognitive impairment versus individuals with dementia or incipient dementia. Method: Enrolled subjects belong to the Long Life Family Study (LLFS), a multi-generational cohort study, where enrollment was predicated upon exceptional family longevity. Included subjects had valid cognitive and telomere data at baseline. Exclusion criteria were age ≤ 60 years, outlying LTL, and missing sociodemographic/clinical information. Analyses were performed using linear regression with generalized estimating equations, adjusting for sex, age, education, country, generation, and lymphocyte percentage. Results: Older age and male gender were associated with shorter LTL, and LTL was significantly longer in family members than spouse controls (p < 0.005). LTL was not associated with working or episodic memory, semantic processing, and information processing speed for 1613 cognitively unimpaired individuals as well as 597 individuals with dementia or incipient dementia (p < 0.005), who scored significantly lower on all cognitive domains (p < 0.005). Conclusions: Within this unique LLFS cohort, a group of families assembled on the basis of exceptional survival, LTL is unrelated to cognitive ability for individuals with and without cognitive impairment. LTL does not change in the context of degenerative disease for these individuals who are biologically younger than the general population.
The regulation of Yorkie, YAP and TAZ: new insights into the Hippo pathway

ABSTRACT The Hippo pathway is a highly conserved signalling pathway that regulates multiple biological processes, including organ size control and cell fate. Since its discovery, genetic and biochemical studies have elucidated several key signalling steps important for pathway activation and deactivation. In recent years, technical advances in microscopy and genome modification have allowed new insights into Hippo signalling to be revealed. These studies have highlighted that the nuclear-cytoplasmic shuttling behaviour of the Hippo pathway transcriptional co-activators Yorkie, YAP and TAZ is far more dynamic than previously appreciated, and YAP and TAZ are also regulated by liquid-liquid phase separation. Here, we review our current understanding of Yorkie, YAP and TAZ regulation, with a focus on recent microscopy-based studies. Summary: Focussing on recent microscopy-based studies, this Review discusses current understanding of the regulation of Yorkie, YAP and TAZ, which are key transcriptional regulators that act downstream of the Hippo pathway to control cell fate and organ size.
In Vitro Estimation of the Effect of Grinding on Rumen Fermentation of Fibrous Feeds

Simple Summary Intensive feeding systems for beef production are based on high proportions of concentrate at the expense of forages, which can lead to digestive disorders. However, the particle size of the different fibrous feeds can also affect the rumen fermentation pattern, and thus animal performance. Fermentation of six fiber sources (soybean hulls, sugarbeet pulp, palm kernel cake, oat hulls, dehydrated alfalfa meal, and barley straw) in two presentation forms (non-processed and ground) was studied in a closed batch in vitro system. Higher gas production was recorded when substrates were presented in ground form, except for barley straw; however, substrates ranked in the same order irrespective of their presentation form. The particle size did not markedly affect volatile fatty acids proportions. Methane production as an index of fermentation efficiency did not show major differences between feed presentation forms, or non-forage substrates as compared with straw and is related more with the magnitude of fermentation than with qualitative changes in fermentation. Considering other feed components, the comparison of substrates on rumen microbial fermentation depends not only on their fiber proportion but can also be mediated by their levels of protein and fat. Abstract The fermentation patterns of six fiber sources, soybean hulls (SH), sugarbeet pulp (BP), palm kernel cake (PK), oat hulls (OH), dehydrated alfalfa meal (DA), and barley straw (BS) were evaluated for this study on the effect of their presentation form (non-processed, NP and ground, GR). Substrates were tested in a conventional in vitro batch system, using rumen fluid obtained from ewes fed 0.5 alfalfa hay and 0.5 barley straw. All substrates rendered a higher gas production in GR form (p < 0.05) except for BS but ranked similarly irrespective of the presentation form. Among the substrates, when incubated NP, the highest volume of gas was recorded with BP from 8 h onwards (p < 0.05), whereas OH and BS resulted in the lowest gas volume (p < 0.05). During the first half of the incubation period, methane production was higher in GR than NP (p < 0.05). Among substrates, despite NP or GR, methane production with BP was the highest (p < 0.05). Similarly, the presentation form did not qualitatively affect fermentation, as no differences were observed in volatile fatty acids proportions. The effect of particle size of fibrous substrates does not have a major impact on the rate and extent of the rumen microbial fermentation.
Policy Roles in Promoting Affordable Housing for Survivors of Intimate Partner Violence

Survivors of intimate partner violence (IPV) face housing insecurity when escaping violence. The Violence Against Women Act (VAWA) and Homeless Emergency Assistance and Rapid Transition to Housing (HEARTH) Act policies were put in place to protect this population. This article uses policy analysis to understand the provisions they provide for survivors. The case of a small city will be presented to understand the local implementation of these policies with regard to survivors’ access to affordable housing in tight housing markets. Finally, recommendations will be presented to strengthen the policies to better meet IPV survivors’ housing needs.
Compilation of anatomical, physiological and metabolic characteristics for a Reference Asian Man-IAEA-TECDOC-1005

A study has been made to obtain/collect sex specific data on human physical parameters of Bangladeshi population of 9 age groups covering all ages for establishing a Bangladeshi/Asian Reference Man for radiation protection purposes. Eleven physical parameters were considered for measurement, namely height and weight of the total body, sitting height, chest girth, combined height of head and neck, head circumference, head width, neck circumference, length of arm, circumference of arm and length of leg. Significant variation in values of respective parameters was observed in most cases between male and female populations of the same age group. The measured values were compared with those of ICRP Reference Man of Caucasian origin. It is observed that the values of physical parameters of Caucasian Reference Man are systematically and probably significantly higher than those of the corresponding Bangladeshi Reference Man. The weights of male and female population of Caucasian Reference Man are approximately 20-30% higher than those of the corresponding Bangladeshi man. Since the organs of the body are roughly proportional to the body weight, the respective masses of the organ would be different by the same ratio factor and could be used for internal dosimetry purposes.
Misleading Epidemiological and Statistical Evidence in the Presence of Simpson's Paradox: An Illustrative Study Using Simulated Scenarios of Observational Study Designs

This study empirically illustrates the mechanism by which epidemiological effect measures and statistical evidence can be misleading in the presence of Simpson's paradox and identify possible alternative methods of analysis to manage the paradox. Three scenarios of observational study designs, including cross-sectional, cohort, and case-control approaches, are simulated. In each scenario, data are generated, and various methods of epidemiological and statistical analyses are undertaken to obtain empirical results that illustrate Simpson's paradox and mislead conclusions. Rational methods of analysis are also performed to illustrate how to avoid pitfalls and obtain valid results. In the presence of Simpson's paradox, results from analyses in overall data contradict the findings from all subgroups of the same data. This paradox occurs when distributions of confounding characteristics are unequal in the groups being compared. Data analysis methods which do not take confounding factor into account, including epidemiological 2×2 table analysis, independent samples t-test, Wilcoxon rank-sum test, chi-square test, and univariable regression analysis, cannot manage the problem of Simpson's paradox and mislead research conclusions. Mantel-Haenszel procedure and multivariable regression methods are examples of rational analysis methods leading to valid results. Therefore, Simpson's paradox arises as a consequence of extreme unequal distributions of a specific inherent characteristic in groups being compared. Analytical methods which take control of confounding effect must be applied to manage the paradox and obtain valid research evidence regarding the causal association.
A Sequence-Based Predictor of Zika Virus Proteins developed by Integration of PseAAC and Statistical Moments.

BACKGROUND ZIKV has been a well-known global threat, which hit almost all of the American countries and posed a serious threat to the entire globe in 2016. The first outbreak of ZIKV was reported in 2007 in the Pacific area followed by another severe outbreak, which occurred in 2013/2014 and subsequently, ZIKV spread to all other Pacific islands. A broad spectrum of ZIKV associated neurological malformations in neonates and adults have driven this deadly virus into the limelight. Though tremendous efforts have been focused on understanding the molecular basis of ZIKV, the viral proteins of ZIKV have still not been studied, extensively. OBJECTIVES Herein, we report the first and the novel predictor for identification of ZIKV proteins. METHOD We have employed Chou's pseudo amino acid composition (PseAAC), statistical moments and various position-based features. RESULTS The predictor is validated through 10-fold cross-validation and Jackknife testing. In 10-fold cross-validation, 94.09% accuracy, 93.48% specificity, 94.20% sensitivity and 0.80 MCC was achieved, while in Jackknife testing, 96.62% accuracy, 94.57% specificity, 97.00% sensitivity and 0.88 MCC was achieved. CONCLUSION Thus, ZIKVPred-PseAAC can help in predicting the ZIKV proteins in an efficient and accurate way and can provide baseline data for the discovery of new drugs and biomarkers against ZIKV.
Building the lac Operon: A Guided-Inquiry Activity Using 3D-Printed Models

Undergraduate biology courses rely heavily on visual representation of information. Students view images of plants, animals, and microbes, interpret data presented in graphs, and use drawings to understand how cells and molecules interact in three dimensions. Traditional teaching approaches exclude students with visual impairments and disadvantage students with disabilities that affect their interpretation and processing of visual and spatial information, and also students who simply do not identify as “visual learners. ” By using new technologies to develop tactile teaching tools (TTTs) that can be employed in classrooms, we aim to create inclusive learning environments and more effectively instruct diverse learners. The advent of affordable and accessible 3D printing technology makes it possible to create tactile models that represent molecules, cells, and entire organisms more accurately than traditional visual representations. We describe the assessment of a 3D gene expression puzzle as a guided inquiry learning activity in which students must correctly assemble a series of components in order to achieve an output. Upon completion of the puzzle, the TTT provides tactile feedback through vibration to signal transcriptional activation. Analysis of pre- and postassessment performance demonstrated statistically significant increases in individual students’ paired assessment scores in two different classroom implementations, with a greater effect size at a rural minority-serving institution than an urban R1 university. These encouraging preliminary data suggest that TTTs with guided-inquiry learning disproportionately benefit disadvantaged student populations and could serve as a tool in leveling the playing field when teaching abstract biological concepts in diverse educational settings.
College students’ intentions to assist peers with chronic medical conditions

Abstract Objective This study identified influences on college students’ intentions to assist peers with chronic medical conditions. Participants: A panel of 293 U.S. full-time college students completed online surveys in July, 2017. Methods: Participants reported the number of people they knew with chronic medical conditions, and completed measures of general empathy, stigma toward chronic conditions, self-efficacy to provide support, and expected likelihood of assisting a peer with a chronic medical condition. Path Analysis and mediation tests were performed. Results: Low stigma, and high confidence in providing support were directly associated with intentions to assist student peers if needed. Empathy and number of people known with chronic conditions were additional indirect predictors. Conclusions: Peer support is important for students with chronic medical conditions. Intention to provide assistance if needed is partially explained by holding low stigma and high confidence in providing support, both of which may be enhanced through education and intervention.