Miracle-dz/UniFAR-FTUs huggingface dataset:
The Fission Yeast S-Phase Cyclin Cig2 Can Drive Mitosis

Commitment to mitosis is regulated by cyclin-dependent kinase (CDK) activity. In the fission yeast Schizosaccharomyces pombe, the major B-type cyclin, Cdc13, is necessary and sufficient to drive mitotic entry. Furthermore, Cdc13 is also sufficient to drive S phase, demonstrating that a single cyclin can regulate alternating rounds of replication and mitosis and providing the foundation of the quantitative model of CDK function. It has been assumed that Cig2, a B-type cyclin expressed only during S-phase and incapable of driving mitosis in wild-type cells, was specialized for S-phase regulation. Here, we show that Cig2 is capable of driving mitosis. Cig2/CDK activity drives mitotic catastrophe—lethal mitosis in inviably small cells—in cells that lack CDK inhibition by tyrosine-phosphorylation. Moreover, Cig2/CDK can drive mitosis in the absence of Cdc13/CDK activity and constitutive expression of Cig2 can rescue loss of Cdc13 activity. These results demonstrate that in fission yeast, not only can the presumptive M-phase cyclin drive S phase, but the presumptive S-phase cyclin can drive M phase, further supporting the quantitative model of CDK function. Furthermore, these results provide an explanation, previously proposed on the basis of computational analyses, for the surprising observation that cells expressing a single-chain Cdc13-Cdc2 CDK do not require Y15 phosphorylation for viability. Their viability is due to the fact that in such cells, which lack Cig2/CDK complexes, Cdc13/CDK activity is unable to drive mitotic catastrophe.
Chapter 2 High-Dimensional Modeling for Cytometry : Building Rock Solid Models Using GemStone TM and Verity Cen-se ’ TM High-Definition t-SNE Mapping

This chapter outlines how to approach the complex tasks associated with designing models for highdimensional cytometry data. Unlike gating approaches, modeling lends itself to automation and accounts for measurement overlap among cellular populations. Designing these models is now easier because of a new technique called high-definition t-SNE mapping. Nontrivial examples are provided that serve as a guide to create models that are consistent with data.
Male Reproductive Success and Intrasexual Selection in the Common Lizard Determined by DNA-microsatellites

Abstract The common lizard (Lacerta vivipara) is a small, nonterritorial, live-bearing lacertid that is sexually dimorphic in several morphological traits (e.g., tail length, snout–vent length, head size). Using microsatellites, we examined paternity in a wild population and investigated whether sexual dimorphism could be the result of intra- or intersexual selection. We found multiple paternity in 65.4% of 26 clutches. There was no evidence of assortative mating. Successfully reproducing males were larger and heavier and had longer tail regenerates or intact tails compared to those that did not reproduce. Tail length and body condition of males were related to the number of offspring sired. However, we found no evidence that head width was related to male reproductive success. We conclude that (1) males with higher body condition index might be more successful in male-male interactions or might be able to search more effectively for females, (2) sex divergence in relative tail length in common lizards reflects the action of sexual selection for male reproductive success, and (3) intersexual dietary divergence could be an alternative hypothesis for head size difference between sexes rather than intrasexual selection.
Phytophthora cinnamomi Colonized Reclaimed Surface Mined Sites in Eastern Kentucky: Implications for the Restoration of Susceptible Species

Appalachian forests are threatened by a number of factors, especially introduced pests and pathogens. Among these is Phytophthora cinnamomi, a soil-borne oomycete pathogen known to cause root rot in American chestnut, shortleaf pine, and other native tree species. This study was initiated to characterize the incidence of P. cinnamomi on surface mined lands in eastern Kentucky, USA, representing a range of time since reclamation (10, 12, 15, and 20 years since reclamation). Incidence of P. cinnamomi was correlated to soil properties including overall soil development, as indicated by a variety of measured soil physical and chemical parameters, especially the accumulation of soil organic carbon. P. cinnamomi was detected in only two of the four sites studied, aged 15 and 20 years since reclamation. These sites were generally characterized by higher organic matter accumulation than the younger sites in which P. cinnamomi was not detected. These results demonstrate that P. cinnamomi is capable of colonizing reclaimed mine sites in Appalachia; additional research is necessary to determine the impact of P. cinnamomi on susceptible tree species at these sites.
Tests for the presence of koi herpesvirus (KHV) in common carp (Cyprinus carpio carpio) and koi carp (Cyprinus carpio koi) in the Czech Republic

An outbreak of koi herpesvirus (KHV) infection associated with high mortality of common and koi carp was recorded in the USA and Israel in 1998. At present, this disease is viewed as one of the most signifi - cant factors that can adversely affect common and koi carp breeds. The disease has spread worldwide including European countries neighbouring with the Czech Republic (CR), i.e. Germany, Poland, and Austria. To monitor the situation in the CR, samples were collected from a total of 138 common and koi carps in seven and eight locations in CR respectively, and were examined between 2005 and 2006. Locations owned by the major produc - ers of common and koi carp were selected with respect of potential occurrence of the KHV virus. No records of increased mortality and morbidity were noticed there. Preferentially carps with non-specific symptoms of disease were sampled, often with isolated skin erosions. To obtain detailed picture about health condition of examined fish the bacteriological and haematological examinations were accomplished. The next part of the examined samples were carp and koi carp for export from professional breeders, imported koi carp and fish from breeds with increased mortality (45 and 21 fish in 2005 and 2006, respectively) The only virological testing was done in this case. The culture and PCR method, according to Gilad et al. (2002) showed negative results for virus KHV in all years. Retrospective investigation by PCR method according to Bercovier et al. (2005) showed positivity in five locations in 2005. In 2006, KHV virus was not detected in any of the selected locations. The aim of our study was to find out the presence/absence of KHV in selected locations and potential correlation of virological, bacteriological and haematological findings. The results of first testing for presence of koi herpesvirus indicates the necessity of regular KHV monitoring in the Czech Republic in the next period.
Drug utilization Study on Oral Hypertensive Medication Patients and Assessment of Medication Adherence to JNC-8 Guidelines in North Indian Tertiary Care Hospital: A Cross-Sectional Study.

Introduction: Hypertension is a common disease which is also known as elevated blood pressure above the normal, i.e., systolic blood pressure above 130 mm Hg and diastolic blood pressure above 90 mm Hg. Hypertension is one of the highest prevailing diseases worldwide. Patients with hypertension are at higher risk of developing stroke, myocardial infarction renal failure as well as various vascular diseases. Near about 75 million of adults in the United States are diagnosed with hypertension according to American Heart Association. Methodology: A drug utilization review on of hypertensive drugs was commenced to determine and evaluate the different classes of antihypertensive medications with respect to diagnosis and ADR’s. Study was commenced in Guru Gobind Singh Medical College and Hospital, Faridkot. Study was conducted for period of 6 months commencing from October, 2015 to March, 2016 as per inclusion exclusion criteria. The inclusion criteria were set to patients suffering from hypertension. Results and conclusion: During the study period and as per inclusion criteria of the study, 150 patients were enrolled out of whom 83 (55.3%) were male and were 67 (44.7%) female irrespective to heredity. The age of patients ranged from 21-85 years with a higher number of the patients belonging to age group ranging between 51-60 years (38.7%). Most of the hypertensive patients were illiterate 83 (55.3%) and unemployed 140 (93.7%) and duration of the disease was less than 5 years in 51 (34%) patients. Among 150 hypertensive patients, most common symptom was found to be breathlessness in 22 (14.7%) patients. Among the hypertensive class, it was found that diuretics were most prescribed drug followed by CCB’s, ACE Inhibitors, β-Blockers, α-β adrenergic antagonist and Angiotensin II receptor antagonistsp
Media Naturalness and Learning STUDYING INVISIBLY : MEDIA NATURALNESS AND LEARNING

This study examines differences between two learning environments: audio-written conferencing and traditional face-to-face instruction. We investigated whether medium richness (Media Richness Theory; Daft & Lengel, 1984), medium naturalness (Media Naturalness Theory; Kock, 2005), and invisibility influence students' achievement, satisfaction, and behavior. In two research settings, a field study and a laboratory experiment, students were taught face-to-face and/or via an audiowritten conferencing system; subject-matter and teacher were constant. We found similar achievement in the two environments. Significant differences, in favor of face-to-face communication, were found regarding learner satisfaction. In addition, invisibility increased certain kinds of students' behavior: participation, risk-taking, immediacy feeling, and flaming. These findings were explained in terms of differences in media naturalness and as an effect of invisibility.
Effects of increased iodine supply on the selenium status of kids

The aim of the study was to monitor the effect of increased iodine supplementation of the clinically healthy kid organism on the selenium status. The study included 7 clinically healthy 14-day-old kids from mothers with high iodine supplementation (group E) and 7 clinically healthy kids from mothers with hypoiodaemia (group C). Kids in group E were administered potassium iodide orally from 14 to 90 days of age. During the experimental period, the group E kids had a total daily iodine intake (from the feeding ration and from the per os potassium iodide administration) of 440-590 µg per head and day in comparison with 140-190 µg per head and day in the group C kids (only from the feeding ration; no potassium iodide administration). In kids of both groups, selenium concentration (Se), glutathione peroxidase activity (GSH-Px), concentration of the thyroid gland hormones (T 3 and T 4 ) and animal weights were monitored. In the group E kids, lower Se concentration (88.1 ± 10.9 µg/l; P < 0.01) and lower activity of GSH-Px (484.0 ± 125.4 µkat/l; P < 0.05) were proved at the end of the experiment (at 105 days of age of the kids) in comparison with the group C kids (131.8 ± 23.2 µg/l and 713.3 ± 153.3 µkat/l, respec- tively). No significant differences were found out in the T 3 or T 4 concentrations or in the weights of animals of both groups. The results indicate that increased iodine supplementation may have a negative effect on selenium metabolism and/or selenium status in kids.
Metabolic rates and swimming performance of adult Fraser River sockeye salmon (Oncorhynchus nerka) after a controlled infection with Parvicapsula minibicornis

Adult sockeye salmon (Oncorhynchus nerka) acquire infections with the myxosporean kidney parasite Parvicapsula minibicornis during their spawning migration in the Fraser River, British Columbia. Controlled infections with this parasite in wild sockeye salmon had no significant impact on plasma ionic status, metabolic rates, and initial maximum prolonged swimming performance (U^sub crit^) for fish ranked as either strongly, weakly, or noninfected by polymerase chain reaction analysis of kidney tissue. However, strongly infected fish had significantly lower second U^sub crit^ and recovery ratio (8%) values, indicating decreased ability to recover from exercise. As the present study shows that the severity of infection is affected by time and temperature, the accumulated thermal units (ATU) of exposure in this study were compared with those experienced by naturally migrating sockeye salmon. A parallel telemetry study revealed that early-timed sockeye experienced significantly more ATU (741.4 ± 29.4 °C) than normally migrating salmon (436.0 ± 20.0 °C) prior to spawning because of a significantly longer holding period in the lake system. The present data are discussed in the context of a threshold of >450 °C ATU for severe infection that would first manifest in earlytimed fish in the upper reaches of the Fraser River and certainly on the spawning grounds. [PUBLICATION ABSTRACT]
Lung disease detection using feature extraction and extreme learning machine

INTRODUCTION: The World Health Organization estimates that by 2030 the Chronic Obstructive Pulmonary Disease (COPD) will be the third leading cause of death worldwide. Computerized Tomography (CT) images of lungs comprise a number of structures that are relevant for pulmonary disease diagnosis and analysis. METHODS: In this paper, we employ the Adaptive Crisp Active Contour Models (ACACM) for lung structure segmentation. And we propose a novel method for lung disease detection based on feature extraction of ACACM segmented images within the cooccurrence statistics framework. The spatial interdependence matrix (SIM) synthesizes the structural information of lung image structures in terms of three attributes. Finally, we perform a classification experiment on this set of attributes to discriminate two types of lung diseases and health lungs. We evaluate the discrimination ability of the proposed lung image descriptors using an extreme learning machine neural network (ELMNN) comprising 4-10 neurons in the hidden layer and 3 neurons in the output layer to map each pulmonary condition. This network was trained and validated by applying a holdout procedure. RESULTS: The experimental results achieved 96% accuracy demonstrating the effectiveness of the proposed method on identifying normal lungs and diseases as COPD and fibrosis. CONCLUSION: Our results lead to conclude that the method is suitable to integrate clinical decision support systems for pulmonary screening and diagnosis.
Comparison Of Performance In Image Restoration By Time And Frequency Domain Techniques

Image Processing is a practice of signal processing aimed at which the input is an image, for instance a photograph or video frame. Also the output of image processing might be either an image or some set of characteristics or restrictions related to the image. An image is defined as an array or a matrix, square pixel decided in rows and columns. Greatest image-processing methods involve giving the image as a twodimensional 2-D signal and applying normal signal processing methods to it. Image processing characteristically refers to digital image processing. The arena of digital image processing mentions to processing digital images by incomes of a digital computer. It comprises many methods they are Image division, Image credit, Image Differencing and Transforming, Digital Compositing, Color Corrections and Image Restoration, etc. In this thesis, a comparison of performance of image restoration is presented to see the behavior of different filters along with diverse coding scheme. Image de-noising is the considered as part of the thesis. In de-blurring, sightless de-convolution is examined. Out of the numerous classes of sightless deconvolution methods, Non parametric approaches based on Image restraints are studied at better depth. A new procedure based on the wavelet method is developed. The algorithm brands use of spatial domain restraints of non-negativity and provision.
Investigating the effect of G-Bond and Z-PRIME Plus on the bond strength between prefabricated zirconia posts and the root canal wall in vitro

Background. The aim of this study was to analyze the effect of G-Bond and Z-PRIME Plus on the bond strength between prefabricated zirconia posts and the root canal wall. Methods. The study was carried out on 21 premolar teeth with similar conditions. The samples were cut at the CEJ. After root canal treatment of the roots, the post space was prepared up to a length of 10mm. The samples were randomly assigned to two groups of 10. G-Bond was used in one group and Z-PRIME Plus in the other to prepare the posts’ surfaces. After cementation and mounting the samples in polyester, the post was cut from the apical area into three equal sections. The bond strength of the samples was tested using the push-out test on a universal testing machine. Data wereanalyzed using t-test. Results. The meanpush-out bond strengths in the control,G-Bond and Z-PRIME Plus groups were 14.3, 27.6±11.8 and 27.4±13.4N, respectively. There was no statistically significant difference between the two groups (P<0.9). Both methods of surface treatment increased bond strength. The bond strengths were different in different sections such as coronal, middle and apical in each group. Conclusion. There was no significant differencebetween the G-Bond and Z-PRIME Plus groups and both products in-creased the bond strength of prefabricated zirconia posts.
Involving male partners in maternity care in Burkina Faso: a randomized controlled trial

Abstract Objective To determine whether an intervention to involve the male partners of pregnant women in maternity care influenced care-seeking, healthy breastfeeding and contraceptive practices after childbirth in urban Burkina Faso. Methods In a non-blinded, multicentre, parallel-group, superiority trial, 1144 women were assigned by simple randomization to two study arms: 583 entered the intervention arm and 561 entered the control arm. All women were cohabiting with a male partner and had a low-risk pregnancy. Recruitment took place at 20 to 36 weeks’ gestation at five primary health centres in Bobo-Dioulasso. The intervention comprised three educational sessions: (i) an interactive group session during pregnancy with male partners only, to discuss their role; (ii) a counselling session during pregnancy for individual couples; and (iii) a postnatal couple counselling session. The control group received routine care only. We followed up participants at 3 and 8 months postpartum. Findings The follow-up rate was over 96% at both times. In the intervention arm, 74% (432/583) of couples or men attended at least two study sessions. Attendance at two or more outpatient postnatal care consultations was more frequent in the intervention than the control group (risk difference, RD: 11.7%; 95% confidence interval, CI: 6.0 to 17.5), as was exclusive breastfeeding 3 months postpartum (RD: 11.4%; 95% CI: 5.8 to 17.2) and effective modern contraception use 8 months postpartum (RD: 6.4%; 95% CI: 0.5 to 12.3). Conclusion Involving men as supportive partners in maternity care was associated with better adherence to recommended healthy practices after childbirth.
Digestibility of total and phytate phosphorus in young calves

Two experiments were conducted to determine digestibility of total and phytate phosphorus (P) in young calves. In the 1st experiment 14 male calves received a commercial milk replacer (6 l of milk per day) and had free access to a starter concentrate. In the 2nd experiment 21 male calves were divided into 3 groups and fed ad libitum a milk replacer (Group I), milk replacer and starter concentrate (Group II), and milk replacer and silaged maize cobs (Group III). Digestibility measurements were carried out at the age of 10 weeks (1st experiment), and 12 and 16 weeks (2nd experiment). In the 1st experiment phytate P accounted for 27.0% of the total P intake. Phytate P was assayed by capillary isotachophoresis. On average, 27.9% of ingested P, but only 3.0% of phytate P were recovered from the faeces. The proportion of phytate P in total faecal P was 5.8%. In the 2nd experiment phytate P accounted for 8.9, 13.8 and 8.6% of total P in diets of calves of Group I, II and III, respectively. On average, 6.6% of ingested P and 3.6% of phytate P were recovered from the faeces. Dry ma�er of faeces contained total P, phytate P and phosphate P at 9.74, 0.65 and 4.69 mg/g, respectively. Faecal concentrations of total P significantly correlated with those of calcium (r = 0.71). It can be concluded that young calves utilised phytate P very efficiently as no more than a few % of phytate escaped degradation in the digestive tract.
Supplementary material for ‘ Selection and estimation for mixed graphical models ’

so the integrability of the conditional density p(xs | x−s) guarantees the integrability of g with respect to xs. Therefore, the conditional densities of the form (3) in the main paper are compatible if θts = θst. We now prove that any function h that is capable of generating the conditional density in (3) of the main paper is in the form (S1). The following proof is essentially the same as that in Besag (1974). Suppose h is a function that is capable of generating the conditional densities. Define P (x) = log{h(x)/h(0)}, where 0 can be replaced by any interior point in the sample space. By definition, P (0) = log{h(0)/h(0)} = 0. Therefore, P can be written in the general form
A HISTOMORPHOMETRIC EVALUATION OF THE EFFECTS OF PLATELET-RICH FIBRIN AND RIFAMYCIN IN COMBINATION WITH AN ALLOGRAFT ON BONE AUGMENTATION WITH SIMULTANEOUS IMPLANT PLACEMENT

Objective: To evaluate the potential of platelet-rich fibrin (PRF) and rifamycin to enhance guided bone augmentation with simultaneous high-profile dental implant placement in a rabbit tibia model. Materials and Methods: Dental implants protruding 2 mm were covered with dome-shaped stiff occlusive titanium barriers filled with demineralized freeze-dried bone allograft (DFDBA)+saline (7 rabbits), DFDBA + rifamycin (8 rabbits), or DFDB +PRF (8 rabbits). After 4 weeks, the animals were sacrificed, and undecalcified histomorphometric examination with toluidine blue staining