Miracle-dz/UniFAR-FTUs huggingface dataset:
The in vitro ToxTracker and Aneugen Clastogen Evaluation extension assay as a tool in the assessment of relative genotoxic potential of e-liquids and their aerosols

Abstract In vitro (geno)toxicity assessment of electronic vapour products (EVPs), relative to conventional cigarette, currently uses assays, including the micronucleus and Ames tests. Whilst informative on induction of a finite endpoint and relative risk posed by test articles, such assays could benefit from mechanistic supplementation. The ToxTracker and Aneugen Clastogen Evaluation analysis can indicate the activation of reporters associated with (geno)toxicity, including DNA damage, oxidative stress, the p53-related stress response and protein damage. Here, we tested for the different effects of a selection of neat e-liquids, EVP aerosols and Kentucky reference 1R6F cigarette smoke samples in the ToxTracker assay. The assay was initially validated to assess whether a mixture of e-liquid base components, propylene glycol (PG) and vegetable glycerine (VG) had interfering effects within the system. This was achieved by spiking three positive controls into the system with neat PG/VG or phosphate-buffered saline bubbled (bPBS) PG/VG aerosol (nicotine and flavour free). PG/VG did not greatly affect responses induced by the compounds. Next, when compared to cigarette smoke samples, neat e-liquids and bPBS aerosols (tobacco flavour; 1.6% freebase nicotine, 1.6% nicotine salt or 0% nicotine) exhibited reduced and less complex responses. Tested up to a 10% concentration, EVP aerosol bPBS did not induce any ToxTracker reporters. Neat e-liquids, tested up to 1%, induced oxidative stress reporters, thought to be due to their effects on osmolarity in vitro. E-liquid nicotine content did not affect responses induced. Additionally, spiking nicotine alone only induced an oxidative stress response at a supraphysiological level. In conclusion, the ToxTracker assay is a quick, informative screen for genotoxic potential and mechanisms of a variety of (compositionally complex) samples, derived from cigarettes and EVPs. This assay has the potential for future application in the assessment battery for next-generation (smoking alternative) products, including EVPs.
Sexual risk behaviors, mental health outcomes and attitudes supportive of wife-beating associated with childhood transactional sex among adolescent girls and young women: Findings from the Uganda Violence Against Children Survey

Compared to young men, Ugandan young women are disproportionately impacted by HIV. Childhood transactional sex may contribute to this disparity. Using data from the 2015 Uganda Violence Against Children Survey, we used logistic regression models to assess the association between childhood transactional sex and negative outcomes. Among 18-24-year-old young women who had sex prior to 18 (n = 982), those who ever engaged in transactional sex had 5.9 times [adjusted odds ratio (AOR); confidence interval (CI): 1.6–22.2] higher odds of having multiple sexual partners in the past year; 5.2 times (AOR; CI: 2.1–12.9) higher odds of infrequent condom use in the past year; 3.0 times (AOR; CI: 1.2–7.9) higher odds of hurting themselves intentionally; and 3.2 times (AOR; CI: 1.3–7.7) higher odds of having attitudes justifying spousal abuse than young women who never engaged in transactional sex. Interventions for transactional sex and HIV in Uganda should consider prioritizing prevention, harm-reduction and continued investment in adolescent girls’ and young women’s futures.
Engineered Aligned Endothelial Cell Structures in Tethered Collagen Hydrogels Promote Peripheral Nerve Regeneration

Vascularisation is important in nerve tissue engineering to provide blood supply and nutrients for long-term survival of implanted cells. Furthermore, blood vessels in regenerating nerves have been shown to serve as tracks for Schwann cells to migrate along and thus form Bands of Büngner which promote axonal regeneration. In this study, we have developed tissue-engineered constructs containing aligned endothelial cells, or co-cultures of both endothelial cells and Schwann cells to test whether these structures could promote regeneration across peripheral nerve gaps. Type I rat tail collagen gels containing HUVECs (Human Umbilical Vein Endothelial Cells, 4×106 cells/ml) were cast in perforated tethering silicone tubes to facilitate cellular self-alignment and tube formation for 4 days of culture. For co-culture constructs, optimal tube formation and cellular alignment was achieved with a ratio of 4:0.5×106 cells/ml (HUVECs:Schwann cells). An in vivo test of the engineered constructs to bridge a 10 mm gap in rat sciatic nerves for 4 weeks revealed that constructs containing only HUVECs significantly promoted axonal regeneration and vascularisation across the gap, as compared to conventional aligned Schwann cell constructs and those containing co-cultured HUVECs and Schwann cells. Our results suggest that tissue-engineered constructs containing aligned endothelial cells within collagen matrix could be good candidates to treat peripheral nerve injury. STATEMENT OF SIGNIFICANCE: Nerve tissue engineering provides a potential way to overcome the limitations associated with current clinical grafting techniques for the repair of severe peripheral nerve injuries. However, the therapeutic cells within engineered nerve tissue require effective vascularisation in order to survive. This work therefore aimed to develop engineered nerve constructs containing aligned tube-like structures made from endothelial cells. Not only did this provide a method to improve vascularisation, it demonstrated for the first time that aligned endothelial cells can outperform Schwann cells in promoting nerve regeneration in the rat sciatic nerve model. This has introduced the concept of developing pre-vascularised engineered nerve tissues, and indicated the potential usefulness of endothelial cell structures in tissue engineering for peripheral nerve repair.
Analysis of spiking synchrony in visual cortex reveals distinct types of top-down modulation signals for spatial and object-based attention

The activity of a border ownership selective (BOS) neuron indicates where a foreground object is located relative to its (classical) receptive field (RF). A population of BOS neurons thus provides an important component of perceptual grouping, the organization of the visual scene into objects. In previous theoretical work, it has been suggested that this grouping mechanism is implemented by a population of dedicated grouping (“G”) cells that integrate the activity of the distributed feature cells representing an object and, by feedback, modulate the same cells, thus making them border ownership selective. The feedback modulation by G cells is thought to also provide the mechanism for object-based attention. A recent modeling study showed that modulatory common feedback, implemented by synapses with N-methyl-D-aspartate (NMDA)-type glutamate receptors, accounts for the experimentally observed synchrony in spike trains of BOS neurons and the shape of cross-correlations between them, including its dependence on the attentional state. However, that study was limited to pairs of BOS neurons with consistent border ownership preferences, defined as two neurons tuned to respond to the same visual object, in which attention decreases synchrony. But attention has also been shown to increase synchrony in neurons with inconsistent border ownership selectivity. Here we extend the computational model from the previous study to fully understand these effects of attention. We postulate the existence of a second type of G-cell that represents spatial attention by modulating the activity of all BOS cells in a spatially defined area. Simulations of this model show that a combination of spatial and object-based mechanisms fully accounts for the observed pattern of synchrony between BOS neurons. Our results suggest that modulatory feedback from G-cells may underlie both spatial and object-based attention.
Hydrogen-Induced Semiconductor Transformation of Lead Zirconate Titanate Ferroelectric Ceramics

Semiconductor transformation of lead zirconate titanate (PZT)-5H ferroelectric ceramics induced by hydrogen has been investigated. The results showed that hydrogen caused the PZT-5H ferroelectric ceramics to transform from a yellow insulator into a black n-type semiconductor, and the leakage current and carrier concentration increased but the resistivity decreased with increasing hydrogen concentration. During charging in H2 at a temperature higher than the Curie point, hydrogen would restrain the PZT-5H ferroelectric ceramics to transform from cubic into tetragonal phase, resulting in the disappearance of ferroelectricity at room temperature. Charging at a temperature below the Curie point, however, did not change the crystal structure of the tetragonal ferroelectric ceramics. The properties and color of PZT-5H ferroelectric ceramics were restored after outgassing at a high temperature.
DRP1 haploinsufficiency attenuates cardiac ischemia/reperfusion injuries

Mitochondrial dynamics is a possible modulator of myocardial ischemia/reperfusion injuries (IRI). We previously reported that mice partially deficient in the fusion protein OPA1 exhibited higher IRI. Therefore, we investigated whether deficiency in the fission protein DRP1 encoded by Dnm1l gene would affect IRI in Dnm1l+/- mouse. After baseline characterization of the Dnm1l+/- mice heart, using echocardiography, electron microscopy, and oxygraphy, 3-month-old Dnm1l+/- and wild type (WT) mice were exposed to myocardial ischemia/reperfusion (I/R). The ischemic area-at-risk (AAR) and area of necrosis (AN) were delimited, and the infarct size was expressed by AN/AAR. Proteins involved in mitochondrial dynamics and autophagy were analyzed before and after I/R. Mitochondrial permeability transition pore (mPTP) opening sensitivity was assessed after I/R. Heart weight and left ventricular function were not significantly different in 3-, 6- and 12-month-old Dnm1l+/- mice than in WT. The cardiac DRP1 protein expression levels were 60% lower, whereas mitochondrial area and lipid degradation were significantly higher in Dnm1l+/- mice than in WT, though mitochondrial respiratory parameters and mPTP opening did not significantly differ. Following I/R, the infarct size was significantly smaller in Dnm1l+/- mice than in WT (34.6±3.1% vs. 44.5±3.3%, respectively; p<0.05) and the autophagic markers, LC3 II and P62 were significantly increased compared to baseline condition in Dnm1l+/- mice only. Altogether, data indicates that increasing fusion by means of Dnm1l deficiency was associated with protection against IRI, without alteration in cardiac or mitochondrial functions at basal conditions. This protection mechanism due to DRP1 haploinsufficiency increases the expression of autophagic markers.
Management of keloid scars: noninvasive and invasive treatments

Scars vary from mature linear scars to abnormal excessive scars such as hypertrophic scars and keloid scars. Keloid scars are fibro-proliferative disease entities that reflect an abnormal process of wound healing. They can cause pain, itching, stiffness, and psychological distress, all of which can affect quality of life. Various treatment options have been advocated as ways to prevent and treat keloid scars. These include noninvasive treatments such as use of silicone gel sheeting and compression therapy, and invasive treatments such as intralesional corticosteroid injections, surgery, and radiotherapy. Novel treatments include chemotherapy, immunotherapy, and anti-inflammatory therapies. Unfortunately, keloids continue to pose a significant challenge due to the lack of efficacious treatments. Therefore, clinicians should be familiar with various therapeutic options and apply the most suitable treatment plan for patients. In this review, we introduce the current therapeutic options for the management of keloid scars.
A cross-talk between CAR T cell subsets and the tumor microenvironment is essential for sustained cytotoxic activity

A cross-talk involving CAR T cell–derived IFN-γ and host-derived IL-12 is essential to maintain CAR T cell killing potential in vivo. CAR T cells collaborate to fight tumors Chimeric antigen receptor (CAR) T cells have achieved clinical success in treating B cell malignancies. Boulch et al. used intravital microscopy to visualize in situ interactions between tumors and anti-CD19 CD4+ or CD8+ CAR T cells and single-cell transcriptomics to examine subsequent changes to the tumor microenvironment in an immunocompetent mouse model of B cell lymphoma. While CD4+ CAR T cells were more effective at stimulating host immune responses, CD8+ CAR T cells excelled at direct tumor cell killing, both of which required CAR T cell–intrinsic IFN-γ expression. Host sensing of IFN-γ and IL-12 production were also required for CAR T cell function, supporting that cross-talk between CAR T cells and the tumor microenvironment is necessary for optimal CAR T cell efficacy against tumors. Chimeric antigen receptor (CAR) T cell therapy relies on the activity of a large pool of tumor-targeting cytotoxic effectors. Whether CAR T cells act autonomously or require interactions with the tumor microenvironment (TME) remains incompletely understood. Here, we report an essential cross-talk between CAR T cell subsets and the TME for tumor control in an immunocompetent mouse B cell lymphoma model of anti-CD19 CAR T cell therapy. Using single-cell RNA sequencing, we revealed substantial modification of the TME during CAR T cell therapy. Interferon-γ (IFN-γ) produced by CAR T cells not only enhanced endogenous T and natural killer cell activity but was also essential for sustaining CAR T cell cytotoxicity, as revealed by intravital imaging. CAR T cell–derived IFN-γ facilitated host interleukin-12 production that supported host immune and CAR T cell responses. Compared with CD8+ CAR T cells, CD4+ CAR T cells were more efficient at host immune activation but less capable of direct tumor killing. In summary, CAR T cells do not act independently in vivo but rely instead on cytokine-mediated cross-talk with the TME for optimal activity. Invigorating CAR T cell interplay with the host represents an attractive strategy to prevent relapses after therapy.
Biology of the Small Diving Beetle Laccophilus yoshitomii Watanabe and Kamite, 2018 (Coleoptera: Dytiscidae) and Rearing Methods

Abstract The dytiscid beetle Laccophilus yoshitomii Watanabe and Kamite, 2018 (Dytiscidae), distributed in Japan, is a small aquatic beetle that was first described in 2018. In the present study a L. yoshitomii population, collected from the type locality (Ishikawa Prefecture, Japan), was reared in the laboratory. The insects laid eggs individually into the main stems of Pogostemon sp. (Lamiaceae). The developmental period for the first, second, and third instar larvae was 2–6 (mean ± SD = 3.5 ± 0.8 days, n = 51), 3–8 (4.2 ± 0.9 days, n = 48), and 5–10 days (6.8 ± 1.3 days, n = 38), respectively; the landing to emergence and landing to escaping the pupal chamber stages lasted 8 (n = 1) and 9–14 days (11.3 ± 1.5 days, n = 13), respectively. A basic rearing model for this species is proposed.
Some Traits of the Biology and Epigean Behavior of Sisyphus schaefferi (Linnaeus) (Coleoptera: Scarabaeidae: Scarabaeinae: Sisyphini)

Abstract A two-year field study on selected traits of the biology of the scarabaeine dung roller Sisyphus schaefferi (Linnaeus) was conducted in a natural park in the western Po Valley in northwestern Italy. Despite laboratory studies carried out over the past few decades, several aspects of the epigean behavior of the species in the wild remain uncertain. Our study revealed that 1) the species is active from early spring to late summer, with reproductive pairs being observed from mid-spring to midsummer; 2) sexual dimorphism in body weight and length is absent; 3) mating individuals are significantly heavier and longer than non-mating individuals; 4) metatibial length increases isometrically with body length and weight; 5) size and weight of the brood balls are isometric with respect to the length and weight of the male of the reproductive pair; 6) brood balls are significantly larger and heavier than food balls, and, whereas brood ball size and weight decrease as air temperature increases, food ball size and weight increase as air humidity rises, but only in non-reproductive periods; 7) pushing and pulling roles are not sex-dependent and can be reversed during the rolling process; 8) combat for balls can involve up to six individuals simultaneously.
IFNAR1 signaling in NK cells promotes persistent virus infection

IFNAR1 signaling in NK cells compromises cellular and humoral immune responses to hamper virus control. Inhibition of type 1 interferon (IFN-I) signaling promotes the control of persistent virus infection, but the underlying mechanisms remain poorly understood. Here, we report that genetic ablation of Ifnar1 specifically in natural killer (NK) cells led to elevated numbers of T follicular helper cells, germinal center B cells, and plasma cells and improved antiviral T cell function, resulting in hastened virus clearance that was comparable to IFNAR1 neutralizing antibody treatment. Antigen-specific B cells and antiviral antibodies were essential for the accelerated control of LCMV Cl13 infection following IFNAR1 blockade. IFNAR1 signaling in NK cells promoted NK cell function and general killing of antigen-specific CD4 and CD8 T cells. Therefore, inhibition of IFN-I signaling in NK cells enhances CD4 and CD8 T cell responses, promotes humoral immune responses, and thereby facilitates the control of persistent virus infection.
Spectrum selection and deep feature fusion based hyperspectral image natural scene classification network

Hyperspectral image classification plays an important role in many remote sensing applications. However, the high-dimensional characteristics of hyperspectral images and the appropriate feature representations leave it with great challenges. In this article, these difficulties are addressed by developing a Spectrum Selection and Deep Feature Fusion based method. The proposed method has the following contributions: 1) reducing redundant information caused by high-dimension through spectrum selection which is just needed in training phase. 2) extracting the joint spectral-spatial features by deep feature fusion, which effectively improves the accuracy of scene classification. 3) increasing the network attention to scene classes of small number by the Class-Balanced loss function and overcome the influence of unbalanced distribution of experimental data. Experiments results in the Tiangong-1 natural scene images dataset (TG1-NSCD) demonstrate that the effectiveness of our algorithm and the OA is 17% higher than the baseline.
Direct investigations of reactions of 1-butoxy and 1-pentoxy radicals using laser pulse initiated oxidation: reaction with O2 and isomerisation at 293 K and 50 mbar

The reactions of 1-butoxy and 1-pentoxy radicals were studied using time-resolved and simultaneous measurement of NO2 and OH concentrations in laser pulse initiated oxidation studies followed by numerical simulations of the concentration profiles. The alkoxy radicals were produced selectively by the excimer-laser photolysis of 1-butyl bromide and 1-pentyl bromide at 248 nm and subsequent reaction of the 1-alkyl radicals with O2 and NO. Whereas NO2 was detected by cw-LIF, OH was monitored by laser long-path absorption at 308 nm. All experiments were performed at 293±3 K and a total pressure of 50 mbar. The reactions with O2 and the isomerisations via a 1,5-H-shift, viz.,
Flexible Work, Temporal Disruption and Implications for Health Practices: An Australian Qualitative Study

Flexible work provisions are justified as enabling workers to manage their personal lives, including their health, around work. This study deploys social theories of practice to investigate how the temporal characteristics of flexible work can produce, alter and disrupt the health improvement efforts of workers, concentrating on healthy eating and keeping physically active. Drawing from in-depth interviews with 12 Australian workers, the study explores the temporal mechanisms linking flexible work to health practices, focusing on routines, rhythms and rituals (the three Rs). This research finds that work-time arrangements can provide the temporal scaffolding necessary for health practices (through routines, rhythms and rituals), but only when there is day-to-day, mid-term, and long-term work predictability. Australia’s flexible work policies do not provide this requisite temporal predictability. Health promoting employment provisions would have to reinstate employment standards from the 1970s, providing the desired predictability for flexible provisions to benefit workers.
Implications of Food Price Shocks on Availability of Food: Evidences from the Indian Economy

In the backdrop of liberalized trade of agricultural commodity in post WTO regime, reform measures is reflected in the change of domestic prices vis-à-vis international prices of food articles. Physical availability of food is purposively selected as a food security indicator in this paper due to its conceptual linkages of food price movement and domestic food supply. A declining trend in the per capita availability of food grains in the post reform period suggests a net export of food grains. Government intervention in procurement and distribution of food grains helps in insulating the shock of food prices in post food crisis situation. Empirical evidences also suggest that there exists a bi-directional causality in between relative price and net export, or food grains production, or government procurement of foodgrains. A responsive change in foodgrains production, net imports, foodgrains procurement and change in the government stock is followed by food price shocks.
The Impact of H2 Addition on Dechlorinating Microbial Communities

ABSTRACT Laboratory-scale column experiments were performed to investigate the effects of membrane-supplied H2 on tetrachloroethene (PCE) dechlorination and microbial community composition. Columns were filled with aquifer material from one of two TCE-contaminated sites and fed a PCE-spiked anaerobic minimal medium for approximately 1 year. For each experiment, one or more experimental columns were supplied with H2 via gas-permeable hollow-fiber membranes with one control column not receiving any H2. After approximately 1 year of operation, aquifer material samples were collected along the length of the columns. Bacterial communities in the samples were analyzed by amplifying the highly variable V3 region of the 16S rRNA gene and separating amplicons using denaturing gradient gel electrophoresis. Microbial community profiles in H2-fed (continuous or pulsed delivery) columns were compared with those in untreated control columns and microbial community profiles were also compared with dechlorination profiles. Selected bands were sequenced for identification. Supply of the simple electron donor H2, changed the microbial community composition, but did not decrease overall diversity. Continuous H2 addition via hollow-fiber membranes enriched for Dehalococcoides-like species, whose relative abundance correlated with enhanced dechlorination activity. PCE was completely dechlorinated to ethene in columns packed with aquifer material from Cape Canaveral, Florida; PCE was dechlorinated to only cis-dichloroethene, however, in columns packed with aquifer material from a TCE-contaminated wetland near Minneapolis, Minnesota. Unexpectedly, Dehalococcoides-like populations were detected in samples from both sets of column experiments. These results suggest that the mere detection of Dehalococcoides-like species in a sample of aquifer material is not a sufficient indicator of the potential to dechlorinate PCE to ethene via biostimulation by H2.