Miracle-dz/UniFAR-FTUs huggingface dataset:
Detection of Virulence and Tetracycline Resistance Determinants in Aeromonas Spps. Isolated From Indian Ruho Carp (Labeorohita) Frys

An increasing incidence of multidrug resistance among Aeromonas spp. isolates, which are fish pathogens and emerging opportunistic human pathogens, has been observed in Indian ruho carp in India. This can be attributed to the horizontal transfer of genetic elements through plasmids. (ompW, aha1, lip) virulent genes (75%, 75%, 25%) were all present in (66.7%), (33.3%) A. sobria, A. hydrophila strains from thirty Indian ruho carp frys from aquaculture ponds respectively, while none of the isolates were bearing to aero virulence gene. One to seven resistant plasmids were isolated from all eight virulent and non virulentAeromonas spp. showed multi-drug resistance with a molecular weight ranging from (1.1-23 kbp), the plasmid content of each isolate was examined using the alkaline lysis protocol. The antimicrobial susceptibilities of 8 Aeromonas spp. isolates from Indian Ruho carp (Labeo rohita) were determined by disc-diffusion testing. Gentamycin, Ciprfloxacin were the drug of choice in combating the bacterial growth in vitro (100%) sensitivity while high levels of resistance against ampicillin were observed (100%) while nalidixic acid and tetracycline showed some level of resistance (57.1%), (28.6%) respectively. tet determinant type was determined by amplification using six degenerate primer sets(tetA, B, C, D, E, G). Genomic and plasmid encoded tetA, tetE were observed while tetG was in coexistence in plasmid DNA only of the same isolate. Moreover tetE in genomic and plasmid born in the same another isolate were observed, However tetB,C,D were not detected in any of the isolates. The results indicate that the pond-raised Ruho carp may be a source of pathogenic Aeromonas spp. and that the potential health risks posed by virulent and multiple antibiotic resistance strains of Aeromonas spp. should be estimated.
Flotation Height Prediction under Stable and Vibration States in Air Cushion Furnace Based on Hard Division Method

Air cushion furnace is indispensable equipment for the production of high quality strip, and it is significant to national economy. The flotation height is a key factor to the quality and efficiency of the product. However, the current prediction models can merely predict the flotation height of strip in air cushion furnace at single working state. The precision of prediction model is inaccurate at the circumstance of low flotation height. To solve the above problem, firstly, this paper proposes a framework which can predict the flotation height of strip under both stable and vibration states. The framework is composed of the hard division model and prediction model. Secondly, a hard division method is proposed based on clustering which combines stacked denoising autoencoder and floating process knowledge. Thirdly, a parallel hybrid flotation height prediction model is proposed, which can provide desirable prediction results at the circumstance of low flotation height. Finally, the LSSVR model is used to predict the maximum and minimum flotation height of strip at vibration state. The experimental results show that the framework can accurately divide the stable and vibration states of the strip and can accurately predict the flotation height of the strip under the stable and vibration states. The research contents of this paper lay an important theoretical foundation for the precise process control in air cushion furnace.
The atheroprotective roles of heart-protecting musk pills against atherosclerosis development in apolipoprotein E-deficient mice.

Background Heart-protecting musk pill (HMP), derived from Chinese herbal medicines, has been found to possess protective roles against atherosclerosis-related cardiovascular diseases (CVDs), however, the anti-atherosclerotic mechanisms of HMP are still unclear. Here, we investigated the effects of HMP on alleviating atherosclerotic lesion severity in mice and explored the molecular mechanisms. Methods Apolipoprotein E-deficient mice were fed western-type diet supplemented with HMP (25 mg/kg/day) or normal saline gavage for 20 weeks. Then histopathological staining was performed to assess the atheromatous plaque burden. Biochemical kits were used to detect levels of lipid profiles. Moreover, effector factors associated with lipid metabolism in liver and intestinal tissues were investigated by western blot and real-time PCR assays. Levels of signal molecules participating in the mitochondrial-mediated apoptosis pathway were detected by Western blot. Results We found that HMP notably reduced atherosclerotic lesion size (P<0.05) and improved plaque stability (P<0.05). HMP treatment decreased circulating TC (P<0.01), LDL-C (P<0.01) and TG (P<0.05) levels and increased HDL-C (P<0.05) content. HMP was found to suppress SREBP2, HMGCR and PCSK9 expressions (P<0.05), yet promote LDLR expression (P<0.05) in hepatocytes. Moreover, HMP was discovered to activate PPARα/CPT-1A cascade (P<0.05) and inhibit contents of SREBP1c and the lipogenic genes FAS and ACCα (P<0.05). The LBK1/AMPK cascade was also activated after HMP administration (P<0.05). Additionally, HMP was found to facilitate transintestinal cholesterol excretion by increasing ABCG5 and ABCG8 levels and reducing NPC1L1 content (P<0.05). In terms of vasoprotective activities, we observed that HMP decreased cleaved caspase-3 content (P<0.05) in the vascular intima, which might be due to inhibition of mitochondrial-related signaling pathway. Conclusions Altogether, our study indicates that HMP plays anti-atherosclerotic roles via regulating lipid metabolism and improving vascular intimal injury.
ASSESSMENT OF GENETIC DIVERSITY OF INDIGENOUS RIDGE GOURD [LUFFA ACUTANGULA (ROXB.) L.] GENOTYPES OF NORTH EAST INDIA AS REVEALED BY SDS-PAGE

Protein content was estimated in seeds of fourty genotypes of ridge gourd. Total seed storage proteins of ridge gourd genotypes analyzed by SDS-PAGE and showed substantial variation in banding pattern of total protein, which varied from 8-24 numbers of bands. The similarity index was calculated on the basis of presence (+) or absence (-) of bands and the dendrogram constructed grouped the 40 genotypes in two major clusters which were further sub divided in four sub clusters. The clustering pattern reflected that the genotype CHFRG35 was most distantly related to CHFRG36 and identified as potential genotypes for crossing programme and to develop new improved cultivars of desired traits. Assessment of genetic diversity of ridge gourd genotypes from various regions of India could be utilised for augmenting the database that would contribute towards systematic breeding programmes and minimizing genetic erosion.
Research on Color Matching Model for Color QR Code

Objective: To provide a faster and more effective way for consumers to obtain the additional information of product, the color quick response (QR) code is printed on product packaging. To improve decoding accuracy of color QR codes. This paper constructs a color matching model with good performance, which restrains aliasing between two color encoding modules of color QR code. Methods: By comparing and analyzing the character of several typical color spaces, the HSV color space was chosen for its favorable attributes. Based on the enlarging capacity principle of color QR code and the maximum rule of relative Euclidean distance, 2n-1 base-color points are set in the HSV space, and 2n-1 pair-color points are calculated by using color distance equation, resulting in a formula capable of encoding 2n colors. Results Results show that the color gamut of QR code pictures generated using our proposed model is wider than that generated using another color matching model. Color aliasing between two encoding modules was greatly restrained. Conclusion The proposed color matching model offers good performance, based upon wider color gamut and reduced aliasing, for generating color QR codes in the HSV color space. Consumers can scan the printed color QR code on product packaging with their electronic devices to obtain more exhaustive information of product effectively.
Title: Short-and long-term reliability of leg extensor power measurement in middle-aged and older adults

Muscular power is important for maintaining physical functioning with aging. Proper quantification of the reliability of muscular power tests is crucial to inform monitoring of individuals and sample size planning for interventional studies. This study evaluated short-and long-term reliability of leg extensor power measurement in 72 adults (age 62.7 ± 8.6 years). Participants completed four repeat trials on the Nottingham leg extensor power rig, with a further trial twelve weeks later. Mean change, typical error, and intraclass correlation coefficients (ICC) were calculated. For short-term reliability, mean change in power output was trivial after two trials (1.2%-4.8%). Typical errors were small following four trials in the dominant leg of males (10.9% to 5.8%), three in the non-dominant leg of males (9.9% to 6.2%) and the dominant leg of females (10.0% to 9.6%) and two in the non-dominant leg in females (8.3%). Intraclass correlation coefficients (ICCs) were very high (0.88-0.96). For long-term reliability, mean change remained trivial (1%-2.5%), typical errors remained small (5.8-8.6%), and ICCs very high (0.94-0.96). The leg extensor power rig is a reliable method for assessing lower body muscular power, both short-and long-term, with only minimal habituation effects.
Obscured flat spectrum radio AGN as sources of high-energy neutrinos

G. Maggi, ∗ S. Buitink, P. Correa, K.D. de Vries, † G. Gentile, 2 J. León Tavares, 4 O. Scholten, 5 N. van Eijndhoven, ‡ M. Vereecken, and T. Winchen Vrije Universiteit Brussel, Dienst ELEM, IIHE, Pleinlaan 2, 1050 Brussels, Belgium Vrije Universiteit Brussel, Department of Physics and Astrophysics, Pleinlaan 2, 1050 Brussels, Belgium Sterrenkundig Observatorium, Universiteit Gent, Krijgslaan 281, 9000 Gent, Belgium Instituto Nacional de Astrof́ısica Óptica y Electrónica (INAOE), Apartado Postal 51 y 216 72000, Puebla, Mexico University of Groningen, KVI-Center for Advanced Radiation Technology, Groningen, The Netherlands Vrije Universiteit Brussel, Dienst ELEM, IIHE and International Solvay Institutes, Pleinlaan 2, 1050 Brussels, Belgium
Prevalence and antimicrobial resistance patterns of E. coli isolates from cow milk, milk products and handlers in the tamale metropolis of Ghana

This study was carried out in the Tamale Metropolis of Ghana to determine the prevalence and antibiotic resistance of Escherichia coli in pasteurized cow milk, cow milk products and hands of cow milk sellers. The conventional method and the disk diffusion method were used for the isolation and antibiotic susceptibility testing of Escherichia coli , respectively. The overall prevalence of Escherichia coli was 42.7% (128/300). Pasteurized milk and raw ‘wagashie’ were the most commonly contaminated (78.0%) source, followed by ‘brukina’ (54.0%), fried ‘wagashie’ (26.0%), left hand (12.0%) and right hand (8.0%). The prevalence of Escherichia coli in pasteurized milk and raw ‘wagashie’ was significantly higher (p = 0.003) than that of ‘brukina’, fried ‘wagashie’, left hand and right hand. Screening of 102 Escherichia coli isolates for their susceptible to antibiotics revealed that 49.0% were susceptible, 31.0% were resistant and 20.0% were intermediate to the various antibiotics post testing. Resistance to ampicillin (65.7%) was the highest, followed by erythromycin (61.8%). The Escherichia coli isolates were generally susceptible to ciprofloxacin (88.2%) and gentamicin (71.6%). The Escherichia coli isolates also exhibited 40 antibiotic resistance patterns with the pattern E (erythromycin) being the commonest. Twenty-nine 29 (28.4%) were resistant to three different classes of antibiotics, 20 (19.6%) were resistant to four different classes of antibiotics, 5 (4.9%) were resistant to five different classes of antibiotics and 1 (1.0%) was resistant to six different classes of antibiotics. The study revealed that milk, its products and handlers in the Tamale metropolis were contaminated with Escherichia coli which are resistant to a number of antibiotics. Keywords: Antibiotics, Escherichia coli , hands, milk, milk products, sellers
OPTIMIZATION OF MICROWAVE ASSISTED DELIGNIFICATION OF WOOD RESIDUES BY SURFACE RESPONSE METHODOLOGY

Efficient processing of vegetal biomass is a great challenge to current research studies. This work is focused on improving the yield of enzymatic hydrolysis of wood residues by removal of lignin using a alkaline wash assisted by microwave heating. The treatments were carried out for one hour in a pressurized microwave reactor (Synthwave-Milestone). The performance of the treatments was assessed by monitoring the concentration of lignin (determined by UV absorbance at 320 nm against a calibration curve). Each experiment was carried out in duplicate. The treatment conditions were established according to an experimental matrix constructed (in Design Expert 11) after the careful selection of the most important factors that affect the lignin removal from wood residue: concentration of NaOH solution, liquid to solid ratio and temperature. A central composite design was constructed with the independent factors mentioned above. ANOVA indicated adequate fitting of the model (correlation coefficient R2=0.95). The exploration of the experimental space (figure 1) with the fitted model indicates the dominant effect of temperature as independent factor. Optimization of experimental conditions within the experimental space was carried according to the following criteria: minimization of temperature, liquid to solid ratio and NaOH concentration and maximization of the response variable, the lignin concentration. The optimal solution (141 mg lignin / g dry wood residue) proposed by the model for these optimization criteria indicates a point in the region determined by the following coordinates: 0.4M NaOH, 1070C and a ratio of liquid to solid equal to 50. Fig. 1. 3D plot of lignin responses surfaces function of the independent factors with significant effectsAcknowledgment The authors acknowledge the financial support received from Competitiveness Operational Program 2014-2020, Priority axis 1, Project No. P_36_611, MySMIS code 107066, Innovative Technologies for Materials Quality Assurance in Health, Energy and Environmental - Center for Innovative Manufacturing Solutions of Smart Biomaterials and Biomedical Surfaces – INOVABIOMED.
ePubWU Institutional Repository

Many publishers use an honor system for selling newspapers in the street. We conducted a field experiment to study honesty in this market, finding that a moral reminder increases the level of honesty in payments, whereas the same message has no effect on whether one is honest. Reminding customers of the legal norm has no effect. We argue that these results are consistent with a preference for honesty, based on an internalized social norm. Auxiliary evidence suggests that the moral message remains effective when it is posted for longer periods, and even when it is removed again. We are grateful for financial support by the Austrian Science Fund (FWF) under Project No. S10307-G14
FLEXURAL BEHAVIOR OF PRESTRESSED CONCRETE BEAMS STRENGTHENED BY NEAR SURFACE MOUNTED FRP RODS

The applications of post tensioned concrete have increased and have now been used indifferent regions of the world. There are only few studies which have studied the behavior ofretrofitted prestressed concrete experimentally, with the major part of the studies focusing onretrofitted reinforced concrete. This research study is initiated by a desire to more fullyinvestigate the response of it. This paper presents a parametric study established on a beamwith T-sec with web width 700 mm and a height of 1800mm, the thickness of the flange is150 mm, the width of beam flange is 1500mm with span 29.6m. The beam was simplysupported and loaded uniformly. A total no. of 20 finite element 3D models was generatedtaking the all possible failure modes such as FRP rupture, concrete cover separation andconcrete crushing in order to study percentage of prestressing steel (Wp) parameter. Finally, acomparison is done between ACI 440.2R-08 code and the finite element results for theultimate failure load and the predicted failure mode 0. تطبیقات الخرسانة سابقة الإجھاد أصبحت واسعة الانتشار فی کل إنحاء العالم وخاصة فی الکباری الخراسانیة ذات البحورالطویلة فی حدود ٣٠ إلی ٤٠ متر لذلک کان لابد من دراسة تدعیم او تقویة ھذا النظام باستخدام الفیبر المسلح المدفونبالقرب من السطح. فی الوقت الحالی کانت غالبیة الدراسات ترکز علی تدعیم الخرسانة المسلحة ویوجد حالیا دراساتبسیطة تطرقت الی ھذا المجال عملیا فقط لذلک ترکزت ھذه الدراسة علی الناحیة النظریة و التمثیل الدقیق لسلوک الکمرةالمدعمة للحصول علی حمل الکسر وتحدید نوع الانھیار. جمیع ھذه الکمرات لھا ارتفاع ثابت ھو ١٨٠٠ مم وعرض جزع T-section تم عمل دراسة نظریة علی کمرة بقطاع الکمرات ٧٠٠ مم وسمک الشفة العلویة (البلاطة) ١٥٠ مم و بعرض ثابت قدره ١٥٠٠ مم . وتم تدعیم ھذه الکمراتبالقضبان البولیماریة المدفونة بالقرب من السطح بإعداد مختلفة وکانت الکمرة بسیطة الارتکاز محملة بحمل موزع. تدرس تأثیر النسبة المئویة لکابلات سابقة الشد فی ABAQUS حیت تم عمل 20 نموذج تحلیلی باستخدام برنامج الACI 440.2R- القطاع. ولکی یتم تقییم النتائج النظریة تم عمل مقارنة حمل الانھیار ونوع الانھیار بین الکود الأمریکی 0.8
Ultraslow Spreading and Volcanism at the Eastern End of Gakkel Ridge, Arctic Ocean

Ultraslow spreading ridges are poorly understood plate boundaries consisting of magmatic and amagmatic segments that expose mostly mantle peridotite and only traces of basalt and gabbro. The slowest part of the global spreading system is represented by the eastern Gakkel Ridge in the Central Arctic Ocean, where crustal accretion is characterized by extreme focusing of melt to discrete magmatic centers. Close to its eastern tip lies the unusual 5,310‐m‐deep Gakkel Ridge Deep (GRD) with limited sediment infill, which is in strong contrast to the broader sediment‐filled rift valleys to the east and west. Here, we report an 40Ar/39Ar age of 3.65 ± 0.01 Ma for a pillow basalt from a seamount located on the rim the GRD, confirming ultraslow spreading rates of ~7 mm/year close to the Laptev Sea as suggested from aeromagnetic data. Its geochemistry points to an alkaline lava, attributed to partial melting of a source that underwent prior geochemical enrichment. We note that the GRD extracts compositionally similar melts as the sparsely magmatic zone further west but at much slower spreading velocities of only ~6−7 mm/year, indicating the widespread occurrence of similarly fertile mantle in the High Arctic. This enriched source differs from subcontinental lithospheric mantle that influences magmatism along the Western Volcanic Zone (Goldstein et al., 2008, https://doi.org/10.1038/nature06919) and is similar to metasomatized mantle, shown to influence melt genesis along the Eastern Volcanic Zone.
Deep Reinforcement Learning with VizDoom First-Person Shooter

In this work, we study deep reinforcement algorithms forpartially observable Markov decision processes (POMDP) combined withDeep Q-Networks. To our knowledge, we are the first to apply standardMarkov decision process architectures to POMDP scenarios. We proposean extension of DQN with Dueling Networks and several other model-freepolicies to training agent using deep reinforcement learning in VizDoomenvironment, which is replication of Doom first-person shooter. We de-velop several agents for the following scenarios in VizDoom first-personshooter (FPS): Basic, Defend The Center, Health Gathering. We com-pare our agent with Recurrent DQN with Prioritized Experience Replayand Snapshot Ensembling agent and get approximately triple increase inper episode reward. It is important to say that POMDP scenario closethe gap between human and computer player scenarios thus providingmore meaningful justification for Deep RL agent performance.
Usable and interactive application generator for Digital TV

Interactive Digital TV (iDTV) enhances viewers’ experience and participation by engaging them with an active role. The development of interactive software requires the employment of usability criteria to provide satisfactory experiences for users. In addition, the diverse characteristics of television programs require efficient processes for interactive software development. In Latin America, the process of implementing DTV is in progress, so the current stage of development of interactive applications is too incipient and immature to support industrial, quality-level development. This paper proposes combining software reuse strategies, specifically, software product lines (SPLs) and user-centered interaction design patterns, to improve productivity and quality. A generic feature model for the automatic generation of iDTV applications and an SPL-iDTV tool that supports the model are presented. The proposal is evaluated with two studies: an experiment that attempts to reproduce real application prototypes that were originally developed manually, through the use of patterns, and an evaluation of the quality of the SPL.
Distinct Populations of Neurons Activated by Heroin and Cocaine in the Striatum as Assessed by catFISH

Despite the still prevailing notion of a shared substrate of action for all addictive drugs, there is evidence suggesting that opioid and psychostimulant drugs differ substantially in terms of their neurobiological and behavioral effects. These differences may reflect separate neural circuits engaged by the two drugs. Abstract Despite the still prevailing notion of a shared substrate of action for all addictive drugs, there is evidence suggesting that opioid and psychostimulant drugs differ substantially in terms of their neurobiological and behavioral effects. These differences may reflect separate neural circuits engaged by the two drugs. Here we used the catFISH (cellular compartment analysis of temporal activity by fluorescence in situ hybridization) technique to investigate the degree of overlap between neurons engaged by heroin versus cocaine in adult male Sprague Dawley rats. The catFISH technique is a within-subject procedure that takes advantage of the different transcriptional time course of the immediate-early genes homer 1a and arc to determine to what extent two stimuli separated by an interval of 25 min engage the same neuronal population. We found that throughout the striatal complex the neuronal populations activated by noncontingent intravenous injections of cocaine (800 μg/kg) and heroin (100 and 200 μg/kg), administered at an interval of 25 min from each other, overlapped to a much lesser extent than in the case of two injections of cocaine (800 μg/kg), also 25 min apart. The greatest reduction in overlap between populations activated by cocaine and heroin was in the dorsomedial and dorsolateral striatum (∼30% and ∼22%, respectively, of the overlap observed for the sequence cocaine–cocaine). Our results point toward a significant separation between neuronal populations activated by heroin and cocaine in the striatal complex. We propose that our findings are a proof of concept that these two drugs are encoded differently in a brain area believed to be a common neurobiological substrate to drug abuse.