Molecular detection of blaOXA-10(OXA-10) type Beta-lactamase encoding gene among extended spectrum Beta-lactamase isolates of Pseudomonas aeruginosa

1 Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq. 2 Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Iraq. * Correspondence: aryan.ganjo@hmu.edu. krd Introduction Antibiotic resistance is a daunting phenomenon with a growing impact on patient safety, particularly for nosocomial infections. The nosocomial strains of P. aeruginosa are frequently resistant to a broad range of commercially available antibiotics. Their prevalence appears to be increasing worldwide, especially as a cause of ventilator-associated pneumonia and in patients with severe burn injuries. Critically ill patients are prone to colonization and infection by antibioticresistant bacteria because of the frequent exposure of these patients to antibiotics. This dangerous array increased the need for broad-spectrum antibiotics, reduced antimicrobial efficacy, and increased antibiotic resistant strains. These infec-tions are complicated to treat due to the occurrence of multidrugresistant (MDR) organisms, which include resistance to beta-lactams, aminoglycosides, fluoroquinolones, and carbapenems. Carbapenems were subsequently introduced as the last resorts of antibiotics due to their high potency and the exceptional broad spectrum of antimicrobial activity, but the carbapenemases, are capable of causing resistance to a wide variety of β-lactam antibiotics, including carbapenems. P. aeruginosa can become resistant to the carbapenems through a number of mechanisms mainly due to the production of OXA-type carbapenemases (class D) Background and objective: Pseudomonas aeruginosa is an opportunistic pathogen and inherently resistant to many antibiotics and can mutate to even more resistant strains during therapy. Resistance to the antibiotics in this group of bacteria increased due to the activity of β-lactamase genes and one of the most important groups of genes, blaOXA gene producing enzymes. The current study aimed to determine the prevalence of Ambler class D β-lactamases, including OXA-10 gene among P. aeruginosa isolated from patients in Erbil, Kurdistan. Methods: Different clinical specimens were taken from patients with clinical symptoms of infection during one year. Identification was carried out on all isolates by Vitek2 system. Antibiotic susceptibility for antimicrobial agents was performed according to the clinical and laboratory standards institute (CLSI) guidelines. Production of Ambler class D β-lactamases was confirmed by polymerase chain reaction technique. Results: A total of 100 isolates of P. aeruginosa, 57 isolates (57%) had shown resistance to six or more than six antibiotics, and 15 isolates showed resistance to one antibiotic. Also, none of the resistant isolates were showed complete resistance to all antibiotics. Out of 89 P. aeruginosa, 38.2% of isolates possessed the blaOXA-10 gene. Conclusion: The results revealed the occurrence of extended-spectrum βlactamases producing Pseudomonas aeruginous, and proper infection control practices are crucial to avert the spreading of ESBL-producing isolates in hospitals.