Miracle-dz/UniFAR-FTUs huggingface dataset:
Genes, environments, and developmental GEWIS: Methods for a multi-site study of early substance abuse

The importance of including developmental and environmental measures in genetic studies of human pathology is widely acknowledged, but few empirical studies have been published. Barriers include the need for longitudinal studies that cover relevant developmental stages and for samples large enough to deal with the challenge of testing gene-environment-development interaction. A solution to some of these problems is to bring together existing data sets that have the necessary characteristics. As part of the NIDA-funded Gene-Environment-Development Initiative (GEDI) our goal is to identify exactly which genes, which environments, and which developmental transitions together predict the development of drug use and misuse. Four data sets were used whose common characteristics include (1) general population samples including males and females; (2) repeated measures across adolescence and young adulthood; (3) assessment of nicotine, alcohol and cannabis use and addiction; (4) measures of family and environmental risk; and (5) consent for genotyping DNA from blood or saliva. After quality controls, 2,962 individuals provided over 15,000 total observations. In the first gene-environment analyses, of alcohol misuse and stressful life events, some significant gene-environment and gene-development effects were identified. We conclude that in some circumstances, already-collected data sets can be combined for gene-environment and gene-development analyses. This greatly reduces the cost and time needed for this type of research. However, care must be taken to ensure careful matching across studies and variables.
DigitalCommons@University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Cougars in Kansas Cougars in Kansas

: The presence of the cougar ( Puma concolor ) in the state of Kansas is a controversial issue. Since 1999, 234 cougar sightings have been reported to the Extension Wildlife Specialist at Kansas State University. To those who have reported such sightings, the existence of cougars in Kansas is undeniable. Others, however, question the validity of such sightings as providing evidence of cougars. After surveying other governmental agencies and organizations, we discovered that acceptable identification criteria for rare or unusual felines included things beside sightings: voucher specimens, DNA from hair or scat, tracks, prey carcasses, and photographs or videos. The cougar sightings from Kansas were plotted on a map using ArcGIS 9.1, and the Spatial Analyst Tool was used to test the 3 hypotheses of: 1) cougar sightings are located near rivers, as cover is provided; 2) sightings of cougars are located around captive felines that may be potential breeding partners; and 3) cougar sightings are located within the vicinity of cities with populations greater than or equal to 35,000 people, as the higher densities of observers may be associated with more opportunities for sightings. Our data led us to accept all 3 hypotheses.
Microbes and Infection Symposium report: One Health meets sequencing --Manuscript Draft--Manuscript

: Whole genome sequencing (WGS) has become the new gold standard for typing and characterization of pathogens. WGS enables understanding transmission chains of bacteria, exchange of mobile genetic elements, and variation in viruses with the highest resolution. In the near future, national and international databases will allow the tracking of pathogen transmissions based on WGS data in and across complex settings such as humans, animals, food, water and other environmental sources. Understanding the complexity and dynamics of and across these compartments will also help us to reduce transmissions of multi-drug resistant pathogens. public This symposium report summarizes sources: local shop-bought raw chicken, frozen meat for “fondue chinoise” (broth fondue), river water, pets, raw milk, and livestock. Our preliminary analysis of 685 genomes, from patients and chicken meat from 2015-2018, shows a large diversity of isolates across 24 clonal complexes. We are most interested in transmission clusters, i.e. patient isolates, which are closely related to those from chicken meat. To identify these, we used a hierarchical approach, first clustering isolates using core genome MLST ((31); then looking within these clusters using a whole genome single nucleotide polymorphism (SNP) approach. Using a SNP cutoff of 12, which was determined as encompassing the vast majority of cases and representing a plausible mutation rate across three years, we can identify 20 transmission clusters including only 14% of cases. This preliminary work indicates that whole genome studies provide a much higher resolution than those using MLST, and that there remain alternative, as yet unidentified infection sources, potentially responsible for up to 86% of campylobacteriosis cases.
Low sul ﬁ de levels and a high degree of cystathionine β -synthase (CBS) activation by S-adenosylmethionine (SAM) in the long-lived naked mole-rat

Hydrogen sul ﬁ de (H 2 S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H 2 S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sul ﬁ de concentration of naturally long-lived species is not known. Here we measured blood sul ﬁ de in the long-lived naked mole-rat and ﬁ ve other mammalian species considerably differing in lifespan and found a negative correlation between blood sul ﬁ de and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β -synthase (CBS), an enzyme whose activity in the liver signi ﬁ cantly contributes to systemic sul ﬁ de levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H 2 S in aging and call for de- tailed research on naked mole-rat transsulfuration.
Transarterial Chemoembolization Combined with Iodine 125 Seeds Versus Transarterial Chemoembolization Combined with Radiofrequency Ablation in the Treatment of Early and Intermediate Hepatocellular Carcinoma

Background To compare the efficacy of the combination of transarterial chemoembolization (TACE) and iodine 125 seeds implantation (TACE-Iodine 125) with the combination of TACE and radiofrequency ablation (RFA) in the treatment of patients with early and intermediate hepatocellular carcinoma (HCC). Methods The study included 134 patients diagnosed with early and intermediate HCC from January 1, 2014, to May 31, 2018. Among them, 47 patients were treated with TACE-Iodine 125, and 87 with TACE-RFA and the efficacy of both treatments was analyzed. To reduced selective bias, propensity score matching (PSM) was used to compare the outcomes of the treatments. Results In the absence of PSM, the median overall survival (OS) and progression-free survival (PFS) of the TACE-RFA group were slightly longer than those of the TACE-Iodine 125 group (OS: 42 months vs. 37 months; PFS: 18 months vs. 15 months). However, there was no significant difference in median OS, PFS, and the objective response rate (ORR) between the two groups (P>0.05). After adjusted for age, gender, liver resection, Child-Pugh class, Barcelona Clinic Liver Cancer (BCLC) stage and Alpha-fetoprotein (AFP), TACE-Iodine 125 treatment was not associated with a significant increasing in risk of death (HR: 0.725; 95%CI: 0.423,1.241, P=0.241) and recurrence (HR: 1.008; 95%CI: 0.666,1.526, P=0.969). After PSM, 47 patient pairs were generated, and there was no significant difference in median OS and PFS between the two groups. Conclusions The combination of TACE and iodine 125 seeds implantation may represent an effective treatment for patients with early and intermediate HCC.
PRBM_A_316048 1011..1018

1Department of Psychology, University of Almería, Almeria, Spain; 2Department of Humanities, University of Almería, Almeria, Spain; 3Department of Psychology, University of Granada, Granada, Spain Introduction: The purpose of the study was to test the factorial validity of Classroom Engagement Inventory of Wang, Bergin and Bergin with Spanish high school students. Methods: In this study, 546 students participated (mean = 13.27, standard deviation = 0.629), from several high schools in Andalusia. To analyze the psychometric properties of the scale, several analyses were carried out. Results: The results offered support for the five-factor structure. The analysis of invariance with respect to gender showed that the factor structure of the questionnaire was invariant. The Cronbach alpha values were higher than 0.70 in the subscales. Discussion: The results of this study demonstrated the reliability and validity of the Spanish version of the Classroom Engagement Inventory with high school students.
CLEP_A_326518 1063..1069

1Department of Dermatology, Aarhus University Hospital, Aarhus, Central Region Denmark, Denmark; 2Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Central Region Denmark, Denmark Purpose: Darier's disease (DD) is a rare genetic skin disease, characterized by yellowbrown, scaly, crusted papules in seborrheic areas and specific nail changes. This study aimed to validate all first-time diagnoses of DD in Danish National Patient Registry (DNPR). The intent of the study is validation of DNPR for epidemiological and clinical studies on DD. Patients and Methods: We identified all patients in DNPR who received their first-time diagnosis of DD between January 1, 1977 and December 31, 2018 (International Classification of Diseases [ICD]-8 code 75721 until the end of 1993: ICD-10 code Q828F thereafter). We restricted to diagnoses from departments of dermatology, where these patients are managed. We validated diagnoses against information from medical records, using predefined data extraction sheets and validation criteria. We classified diagnoses as probable when characteristic clinical features were present; confirmed when there was also genetic confirmation, histopathological confirmation and/or positive family history, or rejected (remaining patients). We estimated positive predictive values (PPVs) with 95% confidence intervals (CIs) for diagnoses overall and stratified by ICD classification, sex, age at diagnosis, year of diagnosis, type of diagnosis, and type of contact. Results: We identified 277 first-time diagnoses of DD, of which 229 (82.7%) stemmed from departments of dermatology. Medical records were available for 196 (85.6%) of these. The overall PPVs for probable and confirmed DD were 89.3% (95% CI: 84.2–92.9) and 81.1% (95% CI: 75.1–86.0), respectively. The PPV for probable ICD-8 diagnosis (95.8% (95% CI: 82.1–99.5)) was slightly higher than that of probable ICD-10 diagnosis (88.4% (95% CI: 82.7–92.3)). Conclusion: The validity of first-time diagnoses of DD recorded by departments of dermatology in the DNPR is relatively high, making DNPR suitable for epidemiological studies on DD in Denmark, as well as a useful source for recruitment to clinical studies on DD.
Assessment of anti nutritional factors and antioxidants in three genotypes of adzuki beans

The basic purpose of the study was to assess and compare the anti nutritional factors and antioxidant activity of the three genotypes of the adzuki bean viz. Local Totru , HPU-51 and EC-340264. Anti nutritional factors like phytic acid and trypsin inhibitors were assessed along with the flavonoid content of the adzuki bean seeds. The phytic acid content of the seeds ranged from 368.96 to 507.92 mg/100g. The percent inhibition of the seeds ranged from the 52 to 77 percent in all the three genotypes. The study can be useful for the producer and consumer to harvest the benefits of adzuki bean based value added products.
LEVEL OF ANTI OXIDANT IN BODY AND ITS EFFECT ON PREGNANCY

: Objective: The main purpose of this research was to know about the value of Total Antioxidant Capacity (TAC) in pregnant women of Pakistan that could be done through the evaluation of TAC in the last three months of an ordinary pregnancy. Methodology: The study was carried out on women with pregnancy and without pregnancy. Both were twenty in numbers. Both types of cases were of same ages. In both types of attendants, TAC was assessed and ideal methods were used to handle the value of TAC. Results: 0.84 mill moles per liter was the TAC value in pregnant females which was 0.16 mill moles per liter less than the standard value (1millimole per liter) among the controls. Conclusion: In the final outcome, we come to know that there was a low value of TAC present in the women of Pakistan. It was the primary report and it will be interrogated further.
CAPABILITY OF FIELD SPECTROSCOPY FOR THE ASSESSMENT OF SOIL CONTAMINATION IN SOUTHERN PORT-SAID GOVERNORATE

: Soil contamination by heavy metals considers as main environmental problem in the world, most of them have toxic effects on plant and microorganisms in soil when permissible concentration levels are exceeded. The objective of the current work is detection of soil contamination in southern Port-said Governorate using spectroscopy Vis-NIR (350-2500 nm). To predict contaminated soil through Vis-NIR 58 different sites were selected in southern Port-said Governorate. Spectroradiometer ASD was used to measure the spectral reflectance of soil site. Three heavy metals Pb, Mn and Cd have been selected to achieve this purpose. Stepwise multiple linear regression (SMLR) was used to construct calibration models. The concentrations of heavy metals were estimated with high accuracy where, the models were validated based on the independent validation. The results illustrated that, R 2 was recorded 0.94, 0.70 and 0.66 for Cd, Mn and Pb respectively. The concentration of soil contamination by Cd, Mn, and Pb are under the threshold. except area contaminated with Cd is (10791.08) faddans. However, Remote Sensing and Geographic Information System (GIS) are provide detailed spatial information on soil contamination , it’s rapidly and inexpensive.
Dynamic Trajectory and Capture of Fine Dust by Magnetic Mesh Filters Based on a Particle Velocity Model

The particle velocity model developed in this study described the dynamic motion of fine dust particles along the magnetized mesh wires in a low Stokes regime. The numerically evaluated velocity contour of the particles modeled the critical path lines indicating the threshold positions for magnetic collection. The velocity contour was determined using the parameters such as particle size, magnetic intensity, flow velocity, and mesh configuration including wire thickness and packing density. Particle size was one of the most significant parameters to determine the magnetic collection. The theoretical estimation matched well with the lab experiments, but the discrepancy increased for large particles with a fine filter. In consequence, application of magnetized mesh filters could contribute to the collection of the ferromagnetic dust or aerosols to a certain extent without any additional power or pressure resistance. Copyright 2015 American Association for Aerosol Research
& (2017). The impact of the pull of the Recent on the fossil record of tetrapods. Evolutionary Ecology Research , 18 (2), 7-23.

15 Question : Why is there an apparently exponential rise in the global diversity of life on 16 land in the past 120 million years? 17 Hypothesis : Most of the apparent rise in diversity is an artefact of improved sampling 18 towards the present day. A particular bias, the Pull of the Recent (POR), affects those fossil 19 taxa with living representatives, by artificially inflating their numbers. 20 Organisms : The fossil record of tetrapods (amphibians, reptiles, mammals and birds). 21 Methods : Comparison of lists of extant families and genera with totals and 22 proportions that do, and do not, have fossil records in the Plio-Pleistocene. 23 Results : The POR extends its influence back to the early Eocene, accounting for at 24 most 6.1% of the increase in tetrapod family diversity and 1.3% of generic diversity. Small 25 animals, insectivores and birds are most affected by the POR, perhaps because of their 26 delicate skeletons. 27 Conclusion : The POR does not significantly distort the pattern of diversification, 28 suggesting that the massive expansion of tetrapod biodiversity in the past 120 myr is largely a 29 real biological pattern. 31
Investigating the Efﬁcacy of Triple Artemisinin-Based Combination Therapies for Treating Plasmodium falciparum Malaria Patients Using Mathematical Modeling

The ﬁrst line treatment for uncomplicated falciparum malaria is artemisinin-based combination therapy (ACT), which consists of an artemisinin derivative coadministered with a longer-acting partner drug. However, the spread of Plasmodium falciparum resistant to both artemisinin and its partner drugs poses a major global threat to malaria control activities. Novel strategies are needed to re-tard and reverse the spread of these resistant parasites. One such strategy is triple artemisinin-based combination therapy (TACT). We developed a mechanistic within-host mathematical model to investigate the efﬁcacy of a TACT (dihydroartemisinin-piperaquine-meﬂoquine [DHA-PPQ-MQ]) for use in South-East Asia, where DHA and PPQ resistance are now increasingly prevalent. Comprehensive model simulations were used to explore the degree to which the underlying resistance inﬂuences the parasitological outcomes. The effect of MQ dosing on the efﬁcacy of TACT was quantiﬁed at various degrees of DHA and PPQ resistance. To incorporate interactions between drugs, a novel model is presented for the combined effect of DHA-PPQ-MQ, which illustrates how the interactions can inﬂuence treatment efﬁcacy. When combined with a standard regimen of DHA and PPQ, the administration of three 6.7-mg/kg doses of MQ was sufﬁcient to achieve parasitological efﬁcacy greater than that currently recommended by World Health Organization (WHO) guidelines. As a result, three 8.3-mg/kg doses of MQ, the current WHO-recommended dosing regimen for MQ, combined with DHA-PPQ, has the potential to produce high cure rates in regions where resistance to DHA-PPQ has emerged. (52). Higher daily doses of MQ are associated with signiﬁcant side effects (53), and thus modeling explored the minimum dosage of MQ that results in optimal efﬁcacy. Hence, the administered dose of MQ was varied, and the corresponding TACT efﬁcacy was estimated. Different scenarios were considered, simulating resistance to DHA and/or PPQ. To simulate different degrees of PPQ and DHA resistance, EC 50 , E max , and W were varied over the limited sampling intervals of the range of values given in Table 3. Since EC 50 is not measurable in experiments, a wide initial range was considered for it: between the concentration that inhibits the growth of parasites by 50% in vitro , IC 50 (adjusted for protein binding), and half the maximum concentration of the median of the PK proﬁles (17). As shown in Fig. 3, the limited sampling intervals for this range are able to produce the probabilities of cure observed in the regions with different levels of parasite susceptibility to drugs.
Density-based trajectory outlier detection algorithm

With the development of global position system (GPS), wireless technology and location aware services, it is possible to collect a large quantity of trajectory data. In the field of data mining for moving objects, the problem of anomaly detection is a hot topic. Based on the development of anomalous trajectory detection of moving objects, this paper introduces the classical trajectory outlier detection (TRAOD) algorithm, and then proposes a density-based trajectory outlier detection (DBTOD) algorithm, which compensates the disadvantages of the TRAOD algorithm that it is unable to detect anomalous defects when the trajectory is local and dense. The results of employing the proposed algorithm to Elk 1993 and Deer 1995 datasets are also presented, which show the effectiveness of the algorithm.
Flexible prior specification for partially identified nonlinear regression with binary responses

Abstract. This paper adapts tree-based Bayesian regression models for estimating a partially identified probability function. In doing so, ideas from the recent literature on Bayesian partial identification are applied within a sophisticated applied regression context. Our approach permits efficient sensitivity analysis concerning the posterior impact of priors over the partially identified component of the regression model. The new methodology is illustrated on an important problem where we only have partially observed data – inferring the prevalence of accounting misconduct among publicly traded U.S. businesses.
Two approaches to state estimation for a class of piecewise affine systems

In this paper we present two approaches to state estimation for a class of discrete time bi-modal piece-wise affine systems. The proposed approaches have the characteristic feature that they do not require information on the currently active dynamics of the piecewise affine system. We propose a Luenberger-type observer, and derive sufficient conditions for the observation error to be globally asymptotically stable, in the case when the system dynamics is continuous over the switching plane. When the dynamics is discontinuous, we derive conditions that guarantee that the estimation error will be bounded with respect to the state bound. Second, we propose to apply particle filtering algorithm, which aims at approximating the a posteriori probability density of the state. The presented approaches are compared and illustrated with examples.
Dopamine Receptor Internalization PSD-95 Protein and Plays a Role in Receptor through 1 with Dopamine D Calcyon Forms a Novel Ternary Complex Cell Biology:

Background: Calcyon has been associated with various dopamine D 1 receptor signalings despite no direct interaction between them. Results: Calcyon forms a novel ternary complex with D 1 DR through PSD-95. Conclusion: Calcyon, by forming a ternary complex, regulates D 1 DR internalization in a phosphorylation-dependent manner. Significance: Regulation of dopaminergic signaling by calcyon (cid:2) PSD-95 (cid:2) D 1 DR complex may represent a novel target for related neuropsychiatric disorders.