Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrative module analysis of HCC gene expression landscapes

Despite hepatocellular carcinoma (HCC) being a common cancer globally, its initiation and progression are not well understood. The present study was designed to investigate the hub genes and biological processes of HCC, which change substantially during its progression. Three gene expression profiles of 480 patients with HCC were obtained from the Gene Expression Omnibus database. Subsequent to performing functional annotations and constructing protein-protein interaction (PPI) networks, 657 differentially expressed genes were identified, which were subsequently used to screen candidate hub genes. PPI networks were modularized using the weighted gene correlation network analysis algorithm, the topological overlapping matrix and the hierarchical cluster tree, which were utilized via STRING. Clinical data obtained from The Cancer Genome Atlas were then analyzed to validate the experiments performed using six hub genes. Additionally, a transcription factor and microRNA-mRNA network were constructed to determine the potential regulatory mechanisms of six hub genes. The results revealed that the oxidation-reduction process and cell cycle associated processes were markedly involved in HCC progression. Six highly expressed genes, including cyclin B2, cell division cycle 20, mitotic arrest deficient 2 like 1, minichromosome maintenance complex component 2, centromere protein F and BUB mitotic checkpoint serine/threonine kinase B, were confirmed as hub genes and validated via experiments associated with cell division. These hub genes are necessary for confirmatory experiments and may be used in clinical gene therapy as biomarkers or drug targets.
Perceived Corporate Reputation and Pride as Drivers of Frontline Employees’ Reputation Impact Awareness: Mediating Role of Job Satisfaction

Purpose – Service employees play a crucial role in creating and sustaining the reputation of service fi rms. Their attitudes and commitment to the fi rm are refl ected in customers’ perceptions of quality and loyalty, which ultimately improve corporate performance. The aim of this study is to better understand what contributes to raising frontline employees’ awareness of their role in building and sustaining the corporate reputation of service fi rms. Design/methodology/approach – We adapt Helm’s (2011) conceptual framework based on a work-related social identity theory and test the adapted model through an empirical study on 544 service fi rms’ frontline employees. Findings – Our fi ndings show that job satisfaction of frontline employees is a signifi cant determinant of their reputation impact awareness, as well as a mediator of Sažetak Svrha – Zaposlenici uslužnih poduzeća imaju ključnu ulogu u stvaranju i održavanju reputacije poduzeća. Stavovi zaposlenih i njihova posvećenost poduzeću odražavaju se u percepcijama korisnika o kvaliteti usluge i lojalnosti, što u konačnici unapređuje performanse poduzeća. Svrha rada jest bolje razumjeti što doprinosi podizanju svijesti kontaktnoga osoblja uslužnih poduzeća o njihovoj ulozi u izgradnji i održavanju korporativne reputacije. Metodološki pristup – Prilagođen je konceptualni okvir koji je razvila Helm (2011), a koji je temeljen na teoriji društvenog identiteta vezanoga uz posao. Prilagođeni je model empirijski testiran na uzorku 544 zaposlenika kontaktne osobe iz uslužnih poduzeća. Rezultati i implikacije – Rezultati istraživanja pokazuju da je zadovoljstvo poslom kontaktnog osoblja značajna odrednica njihove svjesnosti o utjecaju reputacije, kao i Market-Tržište Vol. 31, No. 2, 2019, pp. 171-185 UDK 331.101. 32:316.64/. 65:658:005.336. 6 DOI http://dx.doi. org/10.22598/mt/2019.31. 2.171 Original scientifi c paper Vesna Babić-Hodović, Maja Arslanagić-Kalajdžić 172 Vo l. 31 , N o. 2 , 2 01 9, p p. 1 71 -1 85 the eff ects that pride and perceived corporate reputation have on corporate reputation impact awareness. Limitations – Possible existence of other dimensions of perceived corporate reputation, when it comes to internal stakeholders – the employees, present a limitation of this study and should certainly be considered in future research. Originality/value – We compare and contrast our fi ndings with previous studies and shed more light on internal marketing possibilities targeted towards frontline employees.
Thermodynamic Model

A key step in the photosynthetic reactions in photosystem 11 of green plants is the transfer of an electron from the singlet-excited chlorophyll molecule called P680 to a nearby pheophytin molecule. The free energy difference of this primary charge separation reaction is determined in isolated photosystem 11 reaction center complexes as a function of temperature by measuring the absolute quantum yield of P680 triplet formation and the time-integrated fluorescence emission yield. The total triplet yield is found to be 0.83 ± 0.05 at 4 K, and it decreases upon raising the temperature to 0.30 at 200 K. It is suggested that the observed triplet states predominantly arise from P680 but to a minor extent also from antenna chlorophyll present in the photosystem 11 reaction center. No carotenoid triplet states could be detected, demonstrating that the contamination of the preparation with CP47 complexes is less than 1/100 reaction centers. The fluorescence yield is 0.07 ± 0.02 at 10 K, and it decreases upon raising the temperature to reach a value of 0.05-0.06 at 60-70 K, increases upon raising the temperature to 0.07 at -165 K and decreases again upon further raising the temperature. The complex dependence of fluorescence quantum yield on temperature is explained by assuming the presence of one or more pigments in the photosystem 11 reaction center that are energetically degenerate with the primary electron donor P680 and below 60-70 K trap part of the excitation energy, and by temperature-dependent excited state decay above 165 K. A four-compartment model is presented that describes the observed triplet and fluorescence quantum yields at all temperatures and includes pigments that are degenerate with P680, temperature-dependent excited state decay and activated upward energy transfer rates. The eigenvalues of the model are in accordance with the lifetimes observed in fluorescence and absorption difference measurements by several workers. The model suggests that the free energy difference between singlet-excited P680 and the radical pair state P680+1is temperature independent, and that a distribution of free energy differences represented by at least three values of about 20, 40, and 80 meV, is needed to get an appropriate fit of the data.
AMEP_A_227547 71..77

Point your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: https://youtu.be/iiDTvayaYHU Background: The majority of candidate theses in baccalaureate nursing programs in Sweden are written as literature studies. Being able to carry out a systematic and structured literature search is an essential part of thesis-related work. Aim: The aim of the current study was to investigate changes in nursing students’ search strategies in candidate theses. Methods: A retrospective, quantitative study design was obtained. Librarians (n = 2) and teachers (n = 4) randomly examined selected candidate theses (every third thesis, n = 89) from the years 2012, 2014, and 2016. Results: The result showed a significant improvement over the years (from 2012 and 2014 to 2016) regarding the use of a sufficient number of synonyms, matching search terms to the respective database, use of the Boolean operator OR, and the use of subject headings and free text searches. Use of the title/abstract search largely disappeared. There was a significant change in the types of searches being done. The searches have become more structured in later years as the use of block searches increased significantly; in other words, more systematic and relevant searches have been done in recent years. Conclusion: The result of this study shows that the quality of the students’ search strategies improved significantly during the studied years. It is recommended that search documents are used in both formative and summative assessments to evaluate students’ search strategies. Educational development in the form of enhanced collaboration between librarians and teachers in nursing programs is recommended because it might help to develop student search strategies in literature-based candidate theses.
Extreme Learning Machine with Randomly Assigned RBF Kernels (cid:9)

A new learning algorithm called e xtreme l earning m achine (ELM) has recently been proposed for s ingle-hidden l ayer f eedforward neural n etworks (SLFNs) with additive neurons to easily achieve good generalization performance at extremely fast learning speed. ELM randomly chooses the input weights and analytically determines the output weights of SLFNs. It is proved in theory that ELM can be extended to s ingle-hidden l ayer f eedforward neural n etworks (SLFNs) with r adial b asis f unction (RBF) kernels - RBF networks, which allows the centers and impact widths of RBF kernels to be randomly generated and the output weights to be simply analytically calculated instead of iteratively tuned. The kernel function of ELM can be any nonlinear bounded integrable function which is almost continuous anywhere. Interestingly, the experimental results show that the ELM algorithm for RBF networks can complete learning at extremely fast speed and produce generalization performance very close to that of SVM in some benchmarking function approximation and classiﬁcation problems.
The Impact of Urban Form and Spatial Structure on per Capita Carbon Footprint in U.S. Larger Metropolitan Areas

Different studies have estimated cities’ contribution to total greenhouse gas (GHG) emissions at between forty and seventy percent. According to the so-called Compact City Approach, high density and centrality should lead to low GHG. This study compares the effect of the urban density and spatial structure (monocentrism, polycentrism, and dispersion) of the main U.S. cities on their greenhouse gas emissions from mobility and housing. The estimated models include control variables in order to improve the statistical adjustment, these variables are grouped into three categories: basic controls as temperature and Gross Domestic Product (GDP); historical-demographic controls since 1900; and geographic-urban planning controls. The results detect an environmentally positive effect, albeit a moderate one, associated with monocentric and polycentric spatial structures as compared to dispersed structures. Within the tradition of urban planning, these results can be used as an argument to stop the dispersed decentralization of cities. However, the efficacy of some policies encouraging density should be accompanied by specific policies which increase the energy efficiency of housing and promote the use of public transport.
Source-driven Representations for Hate Speech Detection

English. Sources, in the form of selected Facebook pages, can be used as indicators of hate-rich content. Polarized distributed representations created over such content prove superior to generic embeddings in the task of hate speech detection. The same content seems to carry a too weak signal to proxy silver labels in a distant supervised setting. However, this signal is stronger than gold labels which come from a different distribution, leading to re-think the process of annotation in the context of highly subjective judgments. Italiano. La provenienza di ciò che viene condiviso su Facebook costituisce un primo elemento indentificativo di contentuti carichi di odio. La rappresentazione distribuita polarizzata che costruiamo su tali contenuti si dimostra migliore nell’individuazione di argomenti di odio rispetto ad alternative più generiche. Il potere predittivo di tali embedding polarizzati risulta anche più incisivo rispetto a quello di dati gold standard che sono caratterizzati da una distribuzione ed una annotatione diverse.
Operation modes of the SwissFEL soft X-ray beamline athos

The SwissFEL linac drives the two FEL beamlines Aramis and Athos, a hard and soft X-ray FEL, respectively. The layout of Athos extends from a simple SASE FEL beamline with the addition of delaying chicanes, external seeding and beam manipulation with wakefield sources (dechirper). It also reserves space for a possible upgrade to self-seeding. This presentation gives an overview on the detailed layout enabling the unique operation modes of the Athos facility.
Violaxanthin de-epoxidase and its closest relative: identification and characterization

Light is essential for plants and algae to process photosynthesis. However, excess of light will cause damage to the organism. A process called non-photochemical quenching (NPQ) is an important way for these organisms to protect themselves from photo oxidative damage. The NPQ process is depend on the xanthophyll cycle in thylakoids, which is controlled by the Violaxanthin de-epoxidase (VDE) and zeaxanthin epoxidase (ZE). VDE converts Violaxanthin to to Zeaxanthin on the lumen side of the thylakoid membrane under acid pH conditions caused by photosynthesis. The mature VDE sequence can be divided into three domains, an N-terminal domain with conserved cysteine pattern, a lipocalin-like domain which expected to carry the substrate binding site, and a C-terminal domain rich in glutamic acids. In this work, we have constructed cysteine mutants that revealed that 12 of the 13 cysteines in VDE are essential for the activity; instead of directly contribute to catalytic function, these 12 cysteines formed disultphide bonds for VDE structural folding. The catalytic center of VDE does not appear to be only located in the cysteine-rich N-terminal domain. The expressed N-terminal domain did not show activity and the N-terminal truncation of VDE also loss catalytic ability. When mixing these two separately expressed peptides together, the activity was regained. This shows that these two domains could fold independently to their active folding, and also indicates that the active site may be located in the interface of the two domains. The closest relative of VDE has been found through bioinformatics analysis, the protein has a conserved cysteine pattern as VDE, and named as VDE related protein (VDR). From bioinformatics analysis, some characterization of VDR has been developed; VDR and VDE could be found in same organisms in tree of life, and they are suggested to have the same ancestor; cysteine-rich domain between the two protein are expected to have similar spatial structure, while the corresponding domain of lipocalin-like domain in VDR could have different structure as VDE. With 5’ RACE method and western blot, mRNA and protein level evidence of VDR expression is confirmed, together with differential centrifugation, the localization of VDR in leaf would be suggested to follow chlorophyll.
M[B2(SO4)4] (M = Mn, Zn)—Syntheses and Crystal Structures of Two New Phyllosilicate Analogue Borosulfates

Borosulfates are a rapidly expanding class of silicate analogue materials, where the structural diversity is expected to be at least as large as known for silicates. However, borosulfates with cross-linking of the anionic network into two or even three dimensions are still very rare. Herein, we present two new representatives with phyllosilicate analogue topology. Through solvothermal reactions of ZnO and MnCl2∙4H2O with boric acid in oleum (65% SO3), we obtained single-crystals of Mn[B2(SO4)4] (monoclinic, P21/n, Z = 2, a = 8.0435(4), b = 7.9174(4), c = 9.3082(4) A, β = 110.94(1)°, V = 553.63(5) A3) and Zn[B2(SO4)4] (monoclinic, P21/n, Z = 2, a = 7.8338(4), b = 8.0967(4), c = 9.0399(4) A, β = 111.26(1)°, V = 534.36(5) A3). The crystal structures reveal layer-like anionic networks with alternating vierer- and zwolfer-rings formed exclusively by corner-linked (SO4)- and (BO4)-tetrahedra.
P1.9 The impact of the North Atlantic Oscillation on hydroelectric power generation in the Iberian Peninsula

Over the last few decades the annual consumption of energy in Iberia has never ceased to increase. However, the production of energy has been hampered by technical and environmental constraints. Since the middle of the 20 Century, Portugal and Spain have invested a considerable amount of resources and money to build large dams with hydroelectric production capacities (Melo and Gomes, 1992). More recently, the investment in so called “green energy” has been mainly directed towards the construction of wind farms. However, at the present time, the bulk of this renewable electricity production in both Portugal and Spain still comes from hydroelectric power generated in large dams situated in major river basins. In Portugal the hydroelectric production represents (on an average year of precipitation) one third of the total Portuguese electricity production but its absolute value can vary by a factor of almost three between wet (16 TWh) and a dry (6 TWh) years (Collares Perreira, 1998). Similarly the Spanish hydroelectric production accounts, on average, for 20% of total Spanish production, however the actual production presents a wide range of values, varying between wet (40 TWh) and dry (20 TWh) years (REE, 2002). The western Mediterranean area is characterized by large values of inter-annual variability of precipitation (Esteban-Parra et al, 1998; Trigo and DaCamara, 2000). As a consequence river flow is also characterized by large disparities between wet and dry years especially in southern Iberia. Recently, it has been shown that the North Atlantic Oscilation is the single most important atmospheric circulation mode to control Iberian precipitation (Rodriguez-Puebla et al., 1998; Trigo et al., 2002). Here we intend to show that such influence is extensive to the flow
Kastamonu Eğitim Dergisi

In this study, it was aimed to translate Preference for Solitude Scale developed by Burger (1995) into Turkish and to investigate validity and reliability of the data collected from university students. The validity and reliability studies of the scale was carried out on two different participant groups. The first group was composed of 245 (24.1% male and 75.9% female) and the second group was consisted of 470 (30.2% male and 69.8% female) university students. The results of confirmatory factor analyses in two separate studies showed that the Turkish Form of the scale was composed of 12 items and one dimension as in the original form. According to the reliability studies performed, the Turkish Form of the scale has acceptable internal consistency coefficients and split-half reliability.
Yüzey Yanıt Metodolojisi Kullanılarak Sarı Karpuz Suyunun Bazı Biyoaktif, Duyusal ve Mikrobiyolojik Özelliklere Termosonikasyonun Etkisi

In this study, the application of thermosonication, which is a moderate ultrasound process, on yellow watermelon juice and targeted optimization of the process conditions was completed using the surface response method. For this purpose, yellow watermelon juice was produced and thermosonication at different temperatures (30, 35, 40, 45 and 50 °C), different times (2, 4, 6, 8 and 10 min) and different amplitudes (40%, 45%, 50%, 55% and 60%) at 26 kHz frequency was applied to the samples. Total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (1,1-diphenyl2-picrylhydrazyl (DPPH), cupric reducing antioxidant capacity (CUPRAC)), and color values (L*, a* and b*) were evaluated for optimization of process conditions. At the same time, the differences between pasteurized yellow watermelon juice (PW) and a control (C) of untreated yellow watermelon juice were investigated. At the end of the optimization, microbial safety and sensory properties of the yellow watermelon juice samples were evaluated. As a result of the study, the maximum optimization values for the yellow watermelon juice, with thermosonication applied, were 38.3 °C, 5.6 minutes and 50.5 amplitude. At the end of optimization, CUPRAC (0.214 mg TEAC/mL), DPPH (0.123 mg TEAC/mL), total flavonoid content (41.28 mg CE/L), and total phenolic content (104.30 mg GAE/L) were determined. Thermosonication-treated yellow watermelon juice was found to be safe in terms of microbial values and was most preferred by panelists. As a result, thermosonication technology was successfully used for yellow watermelon juice production. KeywordsYellow Watermelon Juice, Thermosonication, Total Phenolic Content, Total Flavonoid Content, Response Surface Methodology BŞEÜ Fen Bilimleri Dergisi 6 (2), 162-179, 2019 BSEU Journal of Science DOI: 10.35193/bseufbd.559323 e-ISSN: 2458-7575 (http://dergipark.gov.tr/bseufbd)
The Portfolio Optimization Based on Sharp Performance Ratio

In recent years investors evaluate their portfolio using modern portfolio theory developed by Markowitz while in the past they evaluated portfolio types according to the traditional portfolio theory based on simple diversification. In modern portfolio theory, it has been defended that the relationships among financial assets included in the portfolio should be taken into account. In addition, the return and risk of the portfolio can be calculated by the mean-variance model. Investors always expect the maximum return and the minimum risk. Therefore they want to choose the optimum one. In Economics literature there are some measurements to evaluate the performances of the different portfolios. In this study, it is aimed at the portfolio analysis to do for the data of the BIST 30 index. For portfolio optimization, some Artificial Intelligence techniques such as the Genetic Algorithm and Particular Swarm Optimization were used for the data belonging to the year 2018. In these algorithms, different values for the parameters were tried and Sharp Performance Ratio (SPR) was used as a performance criterion. The portfolio found with the maximum SPR has been determined as the optimum portfolio. Finally, the risk and the expected return of the portfolio, the included financial assets and their weights have been obtained. The values of the parameters of the final result are considered as the best.
: Improving the Delayed Stochastic Simulation Algorithm

: In non-delayed stochastic models, while transcription is instantaneous, it takes a considerable time for an RNAp (RNA polymerase) to create an RNA (ribonucleic acid) molecule. In delayed stochastic models, transcription and translation can be represented by a single delayed reaction. Recently, there have been several algorithms for extending the SSA (stochastic simulation algorithm) for incorporate delays (DSSA - delay SSA). Although there is a performance gain of DSSA comparing to SSA for gene transcription and translation, stochastic simulation of biochemical networks has a performance that prevents its use to simulate gene regulatory network on a genome-scale. Therefore, there is a great need to improve the DSSA performance and this paper presents two proposals for that: a new dependency graph and a delayed product list. Palavras-chave
Humanization of healthcare at the emergency department : a qualitative analysis based on nurses ’ experiences

Background: The working conditions of emergency department professionals and the technification of healthcare have had a negative impact on the healthcare professional-patient relationship, dehumanizing it. Objective: This qualitative study explored nurses’ perspectives about the humanization of healthcare in Spanish emergency departments. Methodology: A qualitative design, centred on the opinions of 11 nurses working in the emergency department was applied via semi-structured interviews. Data were analysed using the content analysis technique. Results: Two main themes were identified: The dimensions of humanized healthcare and implementing humanized healthcare at emergency departments, as well as five subthemes. Conclusion: Recommendations are put forward to strengthen initiatives for implementing integrated models of healthcare assistance. The implementation of holistic, patient and family-oriented healthcare is essential to guaranteeing humanized healthcare at the emergency department.