Miracle-dz/UniFAR-FTUs huggingface dataset:
Controllable Drug Delivery by Na+/K+ ATPase α1 Targeting Peptide Conjugated DSPE-PEG Nanocarriers for Breast Cancer

Although Epirubicin (EPI) is a commonly used anthracycline for the treatment of breast cancer in clinic, the serious side effects limit its long-term administration including myelosuppression and cardiomyopathy. Nanomedicines have been widely utilized as drug delivery vehicles to achieve precise targeting of breast cancer cells. Herein, we prepared a DSPE-PEG nanocarrier conjugated a peptide, which targeted the breast cancer overexpression protein Na+/K+ ATPase α1 (NKA-α1). The nanocarrier encapsulated the EPI and grafted with the NKA-α1 targeting peptide through the click reaction between maleimide and thiol groups. The EPI was slowly released from the nanocarrier after entering the breast cancer cells with the guidance of the targeting NKA-α1 peptide. The precise and controllable delivery and release of the EPI into the breast cancer cells dramatically inhibited the cells proliferation and migration in vitro and suppressed the tumor volume in vivo. These results demonstrate significant prospects for this nanocarrier as a promising platform for numerous chemotherapy drugs.
Investigation of β‐hydroxybutyrate in early lactation of Simmental cows: Genetic parameters and genomic predictions

Abstract Genomic information allows for a more accurate calculation of relationships among animals than the pedigree information, leading to an increase in accuracy of breeding values. Here, we used pedigree‐based and single‐step genomic approaches to estimate variance components and breeding values for β‐hydroxybutyrate milk content (BHB). Additionally, we performed a genome‐wide association study (GWAS) to depict its genetic architecture. BHB concentrations within the first 90 days of lactation, estimated from milk medium infrared spectra, were available for 30,461 cows (70,984 records). Genotypes at 42,152 loci were available for 9,123 animals. Low heritabilities were found for BHB using pedigree‐based (0.09 ± 0.01) and genomic (0.10 ± 0.01) approaches. Genetic correlation between BHB and milk traits ranged from −0.27 ± 0.06 (BHB and protein percentage) to 0.13 ± 0.07 (BHB and fat‐to‐protein ratio) using pedigree and from −0.26 ± 0.05 (BHB and protein percentage) to 0.13 ± 0.06 (BHB and fat‐to‐protein ratio) using genomics. Breeding values were validated for 344 genotyped cows using linear regression method. The genomic EBV (GEBV) had greater accuracy (0.51 vs. 0.45) and regression coefficient (0.98 vs. 0.95) compared to EBV. The correlation between two subsequent evaluations, without and with phenotypes for validation cows, was 0.85 for GEBV and 0.82 for EBV. Predictive ability (correlation between (G)EBV and adjusted phenotypes) was greater when genomic information was used (0.38) than in the pedigree‐based approach (0.31). Validation statistics in the pairwise two‐trait models (milk yield, fat and protein percentage, urea, fat/protein ratio, lactose and logarithmic transformation of somatic cells count) were very similar to the ones highlighted for the single‐trait model. The GWAS allowed discovering four significant markers located on BTA20 (57.5–58.2 Mb), where the ANKH gene is mapped. This gene has been associated with lactose, alpha‐lactalbumin and BHB. Results of this study confirmed the usefulness of genomic information to provide more accurate variance components and breeding values, and important insights about the genomic determination of BHB milk content.
Metabolic and molecular signatures of improved growth in Atlantic salmon (Salmo salar) fed surplus levels of methionine, folic acid, vitamin B6 and B12 throughout smoltification

Abstract A moderate surplus of the one carbon (1C) nutrients methionine, folic acid, vitamin B6 and B12 above dietary recommendations for Atlantic salmon has shown to improve growth and reduce hepatosomatic index in the on-growing saltwater period when fed throughout smoltification. Metabolic properties and molecular mechanisms determining the improved growth are unexplored. Here, we investigate metabolic and transcriptional signatures in skeletal muscle taken before and after smoltification to acquire deeper insight into pathways and possible nutrient–gene interactions. A control feed (Ctrl) or 1C nutrient surplus feed (1C+) were fed to Atlantic salmon 6 weeks prior to smoltification until 3 months after saltwater transfer. Both metabolic and gene expression signatures revealed significant 1C nutrient-dependent changes already at pre-smolt, but differences intensified when analysing post-smolt muscle. Transcriptional differences revealed lower expression of genes related to translation, growth and amino acid metabolisation in post-smolt muscle when fed additional 1C nutrients. The 1C+ group showed less free amino acid and putrescine levels, and higher methionine and glutathione amounts in muscle. For Ctrl muscle, the overall metabolic profile suggests a lower amino acid utilisation for protein synthesis, and increased methionine metabolisation in polyamine and redox homoeostasis, whereas transcription changes are indicative of compensatory growth regulation at local tissue level. These findings point to fine-tuned nutrient–gene interactions fundamental for improved growth capacity through better amino acid utilisation for protein accretion when salmon was fed additional 1C nutrients throughout smoltification. It also highlights potential nutritional programming strategies on improved post-smolt growth through 1C+ supplementation before and throughout smoltification.
Breakfast patterns and weight status among adolescents: a study on the Brazilian National Dietary Survey 2008–2009

Abstract Examining the composition of breakfast concerning weight status is essential for evaluating adolescent health and understanding this gap. This study aimed to identify breakfast patterns and investigate the relationship with weight status among Brazilian adolescents. We used a subsample of 7425 adolescents aged 10–19 years from the 2008–2009 Brazilian Household Budget Survey. Breakfast eaters were those with intake of at least 50 kcal (209·2 kJ) between 05.00 and 10.00 hours. Breakfast dietary patterns were derived by principal component factor analysis with varimax rotation. We performed logistic regression analyses between breakfast patterns and weight status, considering the complexity of the survey sample design. Three breakfast patterns were identified explaining 44·8 % of data variability: (1) the Cereal, protein, fruit beverages and Northern/Northeastern pattern, characterised by high consumption of cookies, meats, dairy products, preparations with maize, eggs, fruit juices/fruit drinks/soya-based drinks, tubers/roots/potatoes and cereals, and negative adherence to cold cut meat and savoury snacks/crackers; (2) the Protein-based pattern, characterised by positive loadings for cold cut meat, milk and cheese, and negative for cookies, fruit juices/fruit drinks/soya-based drinks, tubers/roots/potatoes and cereals; and (3) the Mixed pattern, with positive loadings for cakes, coffee/tea, bread, fruit juices/fruit drinks/soya-based drinks, chocolate/desserts and savoury snacks/crackers. No association was found between skipping and weight status. Overweight adolescents had lower adherence to the Cereal, protein, fruit beverages and Northern/Northeastern pattern (OR = 0·67; 95 % CI 0·47, 0·96). This is the first study to address dietary patterns at the meal level with adolescent population-based data, which requires further investigation.
Intravascular Ultrasound Assessment and Correlation With Angiographic Findings of Arterial Dissections Following Auryon Laser Atherectomy and Adjunctive Balloon Angioplasty: Results of the iDissection Auryon Laser Study

Background Femoropopliteal arterial angiographic dissections with the use of the Auryon laser atherectomy system (previously the B-laser) have been infrequent and non–flow limiting. However, the pattern of these dissections (depth and arc) using intravascular ultrasound remains unknown. Materials and Methods We prospectively enrolled 29 patients in the iDissection Auryon study. The primary objective was to define the occurrence of new adventitial injury with intravascular ultrasound (IVUS). Secondary objectives included distal embolization and bailout stenting as judged by the operator because of 30% or more residual narrowing and/or NHLBI (National Heart, Lung, and Blood Institute) angiographic dissection C and higher. Core laboratory analysis was carried on all cases except for 1 patient (that crossed over to Jetstream atherectomy). Dissections were classified according to the iDissection classification as involving the intima (A), media (B), and adventitia (C) and ≤ 180-° arc (1) or >180-° arc (2). Overall, 22 of 29 patients had an embolic filter (per protocol). Results Median lesion and treated lengths were 100.0 and 150.0 mm, respectively. Vessel diameter by IVUS was 6.5 ± 1.5 mm. Chronic total occlusion (CTO) was present in 24.1% of cases. The arc of calcium was: no calcium in 27.6%, <90° in 13.8%, 90° to 180° in 20.7%, and >180° in 34.4%. Lesion severity was reduced to a median of 14% post laser and adjunctive percutaneous transluminal angioplasty (PTA) from a baseline of 76%. Bailout stenting occurred in 6 of 28 (21.4%) patients (3 for dissections, 2 for residual >30%, and 1 for both) and primary stenting in 1 of 28 (3.6%). By IVUS, there were 9 new dissections post laser (1 adventitial; 3≥180°) and 21 new dissections post laser and PTA (3 adventitial; 1≥180°). No distal embolization requiring treatment was seen and no macrodebris ≥2 mm was recovered in the filters. Conclusion The Auryon laser atherectomy system had minimal rate of adventitial injury despite complex disease with relatively low bailout stent rate and no clinically significant macrodebris.
A mean-field approach for modeling the propagation of perturbations in biochemical reaction networks

Often, the time evolution of a biochemical reaction network is crucial for determining the effects of combining multiple pharmaceuticals. Here we illustrate a mathematical framework for modeling the dominant temporal behaviour of a complicated molecular pathway or biochemical reaction network in response to an arbitrary perturbation, such as resulting from the administration of a therapeutic agent. The method enables the determination of the temporal evolution of a target protein as the perturbation propagates through its regulatory network. The mathematical approach is particularly useful when the experimental data that is available for characterizing or parameterizing the regulatory network is limited or incomplete. To illustrate the method, we consider the examples of the regulatory networks for the target proteins c-Myc and Chop, which play an important role in venetoclax resistance in acute myeloid leukemia. First we show how the networks that regulate each target protein can be reduced to a mean-field model by identifying the distinct effects that groups of proteins in the regulatory network have on the target protein. Then we show how limited proteinlevel data can be used to further simplify the mean-field model to pinpoint the dominant effects of the network perturbation on the target protein. This enables a further reduction in the number of parameters in the model. The result is an ordinary differential equation model that captures the temporal evolution of the expression of a target protein when one or more proteins in its regulatory network have been perturbed. Finally, we show how the dominant effects predicted by the mathematical model agree with RNA sequencing data for the regulatory proteins comprising the molecular network, despite the model not having a priori knowledge of this data. Thus, while the approach gives a simplified model for the expression of the target protein, it allows for the interpretation of the effects of the perturbation on the regulatory network itself. This method can be easily extended to sets of target proteins to model components of a larger systems biology model, and provides an approach for partially integrating RNA sequencing data and protein expression data. Moreover, it is a general approach that can be used to study drug effects on specific protein(s) in any disease or condition.
Glyphosate-Based Herbicidal Ionic Liquids with Increased Efficacy

Eight new glyphosate-based herbicidal ionic liquids (HILs), containing both mono- and dianions of glyphosate (benzalkonium glyphosate, bis(2-hydroxyethyl)cocomethylammonium glyphosate, oleylmethylbis(2-hydroxyethyl)ammonium glyphosate, didecyldimethylammonium glyphosate, di(hydrogenated tallow)dimethylammonium glyphosate, 4-decyl-4-ethylmorpholinium glyphosate, di(benzalkonium) glyphosate, and di(bis(2-hydroxyethyl)cocomethylammonium) glyphosate) were prepared via acid–base reaction between the corresponding ammonium hydroxides (some premade) and glyphosate free acid. The transformation of glyphosate free acid into ionic liquids led to an elimination of melting points in all but one compound and significant change in solubilities. All HILs exhibited higher thermal stability than glyphosate free acid. Greenhouse testing indicated that while at a higher application rate of 360 g/ha the efficacy of all the HILs was comparable to the commercial herbicide control, at a lower application rate of 180 g/ha, the e.
Daratumumab in rituximab‐refractory autoimmune haemolytic anaemia

1. Stein EM, DiNardo CD, Pollyea DA, Fathi AT, Roboz GJ, Altman JK, et al. Enasidenib in mutant IDH2 relapsed or refractory acute myeloid leukemia. Blood. 2017;130:722–31. 2. DiNardo CD, Stein EM, de Botton S, Roboz GJ, Altman JK, Mims AS, et al. Durable remissions with ivosidenib in IDH1-mutated relapsed or refractory AML. N Engl J Med. 2018;378:2386–98. 3. Fathi AT, Li S, Soiffer RJ, Levis M, Mims AS, Devine SM, et al. A phase I study of the IDH2 inhibitor enasidenib as maintenance therapy for IDH2mutant myeloid neoplasms following hematopoietic cell transplantation. 2020 Virtual ASH Annual Meeting and Exposition; 2020. 4. Byrne M, Danielson N, Sengsayadeth S, Rasche A, Culos K, Gatwood K, et al. The use of venetoclax-based salvage therapy for post-hematopoietic cell transplantation relapse of acute myeloid leukemia. Am J Hematol. 2020;95:1006–14. 5. Birendra KC, DiNardo CD. Evidence for clinical differentiation and differentiation syndrome in patients with acute myeloid leukemia and IDH1 mutations treated with the targeted mutant IDH1 Inhibitor, AG-120. Clin Lymphoma Myeloma Leuk. 2016;16:460–5. 6. Amatangelo MD, Quek L, Shih A, Stein EM, Roshal M, David MD, et al. Enasidenib induces acute myeloid leukemia cell differentiation to promote clinical response. Blood. 2017;130:732–41. 7. Mason EF, Pozdnyakova O, Roshal M, Fathi AT, Stein EM, Ferrell PB, et al. A novel differentiation response with combination IDH inhibitor and intensive induction therapy for AML. Blood Adv. 2021;5:2279–83.
Layered Orthorhombic Nb2O5@Nb4C3Tx and TiO2@Ti3C2Tx Hierarchical Composites for High Performance Li‐ion Batteries

Engineering electrode nanostructures is critical in developing high‐capacity, fast rate‐response, and safe Li‐ion batteries. This study demonstrates the synthesis of orthorhombic Nb2O5@Nb4C3Tx (or @Nb2CTx) hierarchical composites via a one‐step oxidation —in flowing CO2 at 850 °C —of 2D Nb4C3Tx (or Nb2CTx) MXene. The composites possess a layered architecture with orthorhombic Nb2O5 nanoparticles decorated uniformly on the surface of the MXene flakes and interconnected by disordered carbon. The composites have a capacity of 208 mAh g−1 at a rate of 50 mA g−1 (0.25 C) in 1–3 V versus Li+/Li, and retain 94% of the specific capacity with 100% Coulombic efficiency after 400 cycles. The good electrochemical performances could be attributed to three synergistic effects: (1) the high conductivity of the interior, unoxidized Nb4C3Tx layers, (2) the fast rate response and high capacity of the external Nb2O5 nanoparticles, and (3) the electron “bridge” effects of the disordered carbon. This oxidation method was successfully extended to Ti3C2Tx and Nb2CTx MXenes to prepare corresponding composites with similar hierarchical structures. Since this is an early report on producing this structure, there is much room to push the boundaries further and achieve better electrochemical performance.
Plastic Rewiring of Sef1 Transcriptional Networks and the Potential of Nonfunctional Transcription Factor Binding in Facilitating Adaptive Evolution

Abstract Prior and extensive plastic rewiring of a transcriptional network, followed by a functional switch of the conserved transcriptional regulator, can shape the evolution of a new network with diverged functions. The presence of three distinct iron regulatory systems in fungi that use orthologous transcriptional regulators suggests that these systems evolved in that manner. Orthologs of the transcriptional activator Sef1 are believed to be central to how iron regulatory systems developed in fungi, involving gene gain, plastic network rewiring, and switches in regulatory function. We show that, in the protoploid yeast Lachancea kluyveri, plastic rewiring of the L. kluyveri Sef1 (Lk-Sef1) network, together with a functional switch, enabled Lk-Sef1 to regulate TCA cycle genes, unlike Candida albicans Sef1 that mainly regulates iron-uptake genes. Moreover, we observed pervasive nonfunctional binding of Sef1 to its target genes. Enhancing Lk-Sef1 activity resuscitated the corresponding transcriptional network, providing immediate adaptive benefits in changing environments. Our study not only sheds light on the evolution of Sef1-centered transcriptional networks but also shows the adaptive potential of nonfunctional transcription factor binding for evolving phenotypic novelty and diversity.
Mechanical History Effects in the Crystallization of cis-1,4-Polybutadiene

Abstract Dilatometric studies have revealed alterations in the crystallization kinetics of cis-l,4-polybutadiene subjected to the shearing action of a laboratory mill. Reduction in the Avrami exponent by more than one unit suggests that, in addition to a change from pseudo-homogeneous to instantaneous nucleation, a change in the growth mechanism of the new phase is involved. These changes are presumed to be brought about either by sub-optical microscopic ordering of the amorphous polymer (e.g., the ‘quasi-indestructible’ clusters proposed by Rabesiaka and Kovacs) or by shear effects on. impurity constituents, with either effect leading to row orientation.
Elucidating Skills for Job Seekers: Insights and Critical Concerns from a Field Deployment in Switzerland

This article contributes results of a longitudinal field study of SkillsIdentifier, an employment tool originally designed and assessed in the United States (U.S.), to support “underrepresented” job seekers in identifying and articulating their employment skills. To understand whether the tool could support the needs of job seekers outside the U.S., we assessed it among 16 job seekers with limited education and language resources in Switzerland. While many of our results mirrored those of the U.S., we found that the tool was especially beneficial for non-French speaking immigrants who needed support describing their skills outside of their native language. We also found that listing skills like “active listening” without important context was insufficient and risked hiding key skills and meaning behind those skills to employers. Taking these factors into account, we illustrate the design implications of our findings and directions for practitioners who wish to design employment tools in support of job seekers, especially those who have traditionally been excluded from the labor market. We then provide insight into the potential for unintended consequences as a result of focusing solely on skills in a post-COVID labor market and contribute ways to mitigate them.
Exploring Affordances of Surface Gestures on Textile User Interfaces

This pictorial explores the design space for communicating surface gestures to users of textile interfaces by experimenting with the interfaces’ physical design and affordances. First, we created a collection of functional and non-functional textile samples. Their development was based on three aspects: design, fabrication, and sensing. The design aspect covered different visual (shape, color) and haptic (details, textures) designs, fabrication explored three textile-specific fabrication methods, and electronic sensing offered options for adding touch-sensing capabilities. Second, we reflected on created samples and their characteristics contrasting different designs and speculating on why some work better than others. Our main findings and insights are presented in five clusters: ergonomics, visual affordances, perception of textures, the direction of movement, and the economic usage of design elements. This intermediate-level knowledge can provide a starting point for each professional or novice designer to take inspiration from, when creating their own textile user interfaces.
How HCI Bridges Health and Design in Online Health Communities: A Systematic Review

This paper presents a systematic review of online health communities (OHCs) published between 2009 and 2020 in the ACM Digital Library. Aiming to consolidate the current issues, design knowledge, challenges, and tensions in OHCs, our analysis identified four high-level aspects related to the use and design of OHCs: (1) temporal: OHCs as transition spaces, (2) spatial: bridging experiential knowledge with medical expertise, (3) technological: exchanging and locating peer support, and (4) tension dimensions in OHCs. We further discuss methodological improvements and computing opportunities for OHC research and how to increase OHC members’ agency in such a medically dominated context. These findings have the potential to inform future OHC designs and help researchers and designers position future contributions.
Contemplative Interactions: Exploring the Use of Defamiliarization in a Serious Game to Promote Reflective Thinking about Personal Health

Counsellors use Motivational Interviewing (MI) to boost intrinsic motivation for healthier lifestyles by supporting 1) the identification of personal values and 2) the linking of these values to personal reasons for change. Research is needed to increase the reach of such interventions through the use of online tools. Thus, we explored the use of defamiliarization and poetic gameplay in a serious game, to make habitual health choices difficult and ‘unfamiliar’ in order to promote players’ contemplation about their personal reasons for health. Our evaluation indicates that the players demonstrated evidence of processes of change such as self-reevaluation, environmental reevaluation, and improving decisional clarity. We observed that these methods could promote dialogic reflection with different points of view, but was less effective at promoting transformative reflection that would lead to change. We conclude with a discussion of promising future directions for research.
Discovering the Design Challenges of Autonomous Vehicles through Exploring Scenarios via an Immersive Design Workshop

A major challenge for autonomous vehicles (AVs) today is driving in a complex urban environment where various traffic participants, infrastructures, and events are mixed. A growing body of research is studying the interaction between AVs and human road users (HRUs) to mitigate this challenge. Although traffic is complicated, research has focused on limited situations, such as pedestrian crossings. This study aims to explore scenarios that have a high possibility of causing problems in mixed traffic situations. We devised a design workshop method using miniatures and small cameras, thus allowing participants to experience scenarios from the HRU perspective and easily creating new road situations to uncover various problems. By analyzing 133 scenarios found through the workshop, we defined five factors and 51 elements of AV–HRU problem scenarios. Through qualitative analysis of the factors found through experiments and comparison with existing studies, we identified research gaps and discussed future design challenges.