Miracle-dz/UniFAR-FTUs huggingface dataset:
The Linamarin ,-Glucosidase in Costa Rican Wild Lima Beans (Phaseolus lunatus L.) Is Apoplastic1

Analysis of mesophyll protoplasts and cell wall extracts of leaf discs of Costa Rican wild lima bean (PhkasolNs lunatus L.) shows that the linamarse activity is confned to the apoplast. Its substrate linamarin, together with the related enzyme hydroxynitrile lyase, is found inside the cells. This compartmentation prevents cyanogenesis from occurring in intact tissue, and suggests that linamarin has to be protected during any translocation across the linamarse ich apoplast.
Planning and Control Methods for Robotic Manipulation Tasks with Non-Negligible Dynamics

This thesis presents novel insights and solutions for the planning and control of robotic manipulation tasks with non-negligible dynamics. The central goal is to equip robots with advanced sensory and motor skills. Hereby, it represents an important contribution to the development of flexible and versatile robotic systems. The thesis follows a model-based approach and discusses state-of-the-art models of the system and its environment. Methods for optimal motion planning and for hybrid control of dynamic manipulation tasks are presented and applied to a number of case studies. Specific challenges with respect to environment perception are addressed that are characteristic for dynamic object manipulation. In order to improve the system performance, a compliant end effector design is proposed and evaluated. The effectiveness of the employed methods and the overall framework is demonstrated in a number of simulations and experiments.
Preoperative bowel stimulation prior to ileostomy closure to restore bowel function faster and improve postoperative outcomes: a systematic review

Aim: Closure of a diverting ileostomy following restorative surgery is often associated with significant short-term morbidity and variable long-term bowel function. The aim of this systematic review was to investigate if preoperative stimulation of the defunctioned bowel restores bowel function after ileostomy closure faster and improves postoperative outcomes when compared to standard preoperative care. Method: MEDLINE, Embase, CENTRAL, Google Scholar and ClinicalTrials.gov were searched for studies evaluating preoperative bowel stimulation in patients with a temporary ileostomy after low anterior resection or ileal pouch-anal anastomosis, regardless of their design, publication type or language. Study selection, data
A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein

In previous work, the performance of a compact and cost-effective point-of-care (PoC) device based on the increase relative optical power (IROP) methodology is reported and it is determined the enhancement in comparison with standard high-resolution spectrometry in terms of limit of detection. This paper describes a new label-free IROP-based biomedical device capable of working with low concentration of reagents and low sample volume per measurement in order to be used for screening different steps necessary in immunoassay optimization. This new approach significantly improves the sensing performance in terms of read-out signal ( $\Delta $ IROP) per nanometer of biofilm in comparison with our previous work. This improvement is achieved due to the implementation of a laser as light source of the optical read-out system and the redesign of Fabry–Perot transducers by optimizing their reflectivity response and reducing their sensing area. For demonstrating the screening capability of this new PoC device in several immunoassays steps and methodologies, a C-reactive protein detection assay was carried out as a potential application and assay model. It is remarkable that only $10~\mu \text{L}$ of sample was used per measurement. This label-free IROP-based device complies an easy-to-use and cost-effective tool for immunoassays optimization in terms of performance, reagents cost, and measuring time.
Signaling Mechanisms in the Neuronal Networks of Pain and Itch

Rogoz, K. 2012. Signaling Mechanisms in the Neuronal Networks of Pain and Itch. Acta Universitatis Upsaliensis. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 833. 78 pp. Uppsala. ISBN 978-91-554-8517-7. Glutamate is the essential neurotransmitters in pain pathways. The discovery of the vesicular glutamate transporters (VGLUT1-3) has been a fundamental step on the way to describe glutamate-dependent pain pathways. We used the Cre-lox system to construct conditional knockouts with deficient Vglut2 transmission in specific neuronal populations. We generated a Vglut2 line to selectively delete Vglut2 from the peripheral nervous system. These Vglut2 deficient mice showed decreased acute nociceptive responses and were less prone to develop an inflammatory state. They did not develop cold allodynia, or heat hyperalgesia and were less hypersensitive to mechanical stimuli in the PSNL chronic pain model. Further analyses of genes with altered expression after nerve injury, revealed candidates for future studies of chronic pain biomarkers. Interestingly, the Vglut2 mice developed an elevated itch behavior. To investigate more specific neuronal populations, we analyzed mice lacking Vglut2 in the Nav1.8 population, as inflammatory hyperalgesia, cold pain, and noxious mechanosensation have been shown to depend upon Nav1.8 positive sensory neurons. We showed that deleting Vglut2 in Nav1.8 positive neurons abolished thermal hyperalgesia in persistent inflammatory models and responses to noxious mechanical stimuli. We also demonstrated that substance P and VGLUT2-dependent glutamatergic transmission are co-required for the development of formalin-induced inflammatory pain and heat hyperalgesia in persistent inflammatory states. Deletion of Vglut2 in a subpopulation of neurons overlapping with the vanilloid receptor (TRPV1) primary afferents in the dorsal root ganglia resulted in a dramatic increase in itch behavior accompanied by a reduced responsiveness to thermal pain. Substance P signaling and VGLUT2-mediated glutamatergic transmission in TRPV1 neurons was co-required for the development of inflammatory pain states. Analyses of an itch phenotype uncovered the pathway within TRPV1 neurons, with VGLUT2 playing a regulatory role and GRPR neurons, which are to plausible converge the itch signal in the spinal cord. These studies confirmed the essential role of VGLUT2-dependent glutamatergic transmission in acute and persistent pain states and identified the roles of specific subpopulations of primary afferent neurons. Additionally, a novel pain and itch transmission pathway in TRPV1/VGLUT2 positive neurons was identified, which could be part of the gate control of pain.
PRBM_A_282493 1299..1310

1Seoul National University, College of Nursing, The Research Institute of Nursing Science, Seoul, South Korea; 2University of Kentucky, College of Nursing, Lexington, KY, USA Background: Prior studies of symptoms in heart failure (HF) were largely cross-sectional and symptoms were measured using retrospective recall. Because negative emotions influence information processing, retrospective symptom reports by patients with depressive symptoms and anxiety may be biased. The purpose of this study was to determine whether there are differences in patterns of symptom changes, measured prospectively, over 15 days by levels of depressive symptoms and anxiety. Methods: HF patients (N=52) rated daily symptom severity for shortness of breath (SOB), fatigue, sleep disturbance, and edema over 15 days on a 10-point visual analogue scale. Patients were grouped into higher vs lower levels of depressive symptoms and anxiety, respectively, based on median scores of Brief Symptom Inventory subscales. Latent growth curve modeling was used to examine whether patterns of symptom changes over 15 days differed in higher vs lower levels of depressive symptom and anxiety groups. Results: Those in the higher depressive symptom group had lower levels of baseline symptom severity in SOB (β: −1.46), fatigue (β: −1.71), sleep disturbance (β: −1.78), and edema (β: −1.97) than those in the lower depressive symptom group. However, there were no significant differences in rates of changes in the severity of any of the four symptoms between groups. Anxiety was not associated with baseline severity of symptoms or rates of changes in any of the four symptoms. Conclusion: Depressive symptoms, but not anxiety, were associated with daily symptom experience. HF patients with higher levels of depressive symptoms may perceive their symptom severity differently than patients with lower levels of depressive symptoms.
FWDNet: A Novel Recognition Network for Ferrography Wear Debris Image Analysis

Ferrography wear debris in lubricating oil contains abundant worthy information about the state of the machinery and equipment. In order to develop an online monitoring system based on condition maintenance and fault diagnosis, wear debris needs to be identified automatically. Through various tribological experiments, a dataset of seven kinds of wear debris was established. In this study, DenseNet121 was used as the base network to construct a DCNN model (FWDNet) using the transfer learning method. FWDNet obtained an accuracy of 90.15% through a 10-fold crossvalidation test. The results indicate that FWDNet and DCNN mode is suitable for the identification of wear debris and can be used in actual condition monitoring systems in the future.
A BIBLIOMETRIC ANALYSIS OF COHESIVE SPEECH RESEARCH OF PRESCHOOLERS FROM 1970 TO 2020

Cohesive speech has commonly become regarded as one of the essential aspects of language development, especially for pre-schoolers. This study aimed to shed light on the cohesive speech of pre-schoolers (CSP) knowledge base. Three hundred ninety-five publications close related cohesive speech research of 5-6-year-old children were collected from the Scopus database. By using the bibliometric approach, the results showed the growth rate of publications over time from 1970 to 2020. In this period, the top five countries, the United States, the United Kingdom, Australia, Brazil and France, have published over 60% of total documents. Besides, four main interests of authors in this field were discovered: “language development”, “speech pathology outcomes”, “speech therapy”, “language intervention”. Among these themes, “speech pathology outcomes” was the most relevant one. Another finding, in recent years, scholars focused on aspects of autism spectrum disorder of pre-schoolers. More importantly, the achievements of this discipline have considerably made positive contributions to various fields both in terms of theory and practice, especially in language and linguistics, speech and hearing, psychology, and medicine. Overall, these studies highlight the need for many other studies, particularly relevant to pre-schooling when new generations should be better prepared to be happy in school by their future cognitive developments.
USE OF NANOMATERIALS IN INDUCING TOLERANCE AGAINST WATER STRESS IN FAVA BEAN ( VICIA FABA L.) PLANTS GROWN IN TABUK REGION

: In the past decade nanomaterials (NMs) have emerged as important tools for manipulating growth and yield of crop plants. Of these NMs, nano-titanium dioxide (nano-TiO 2 ) has been shown to play various roles in growth and development of crop plants under abiotic stresses. Present work was carried out to evaluate the role nano-TiO 2 in the protection of plants against water stress (WS) and to select the best of nano-TiO 2 . The results show that WS caused a significant reduction in growth attributes, chlorophyll (Chl) content, leaf relative water content (LRWC) and activity of nitrate reductase (NR) enzyme. Whereas, an increase was noticed in reactive oxygen species (ROS), carbonic anhydrase (CA) activity, electrolyte leakage, and lipid peroxidation in water stressed plants. In addition, plants under WS also showed an increase in the activities of antioxidant enzymes and accumulation of proline (Pro) and glycine betaine (GB) content. However, application of nano-TiO 2 (5, 10, 15, and 20 mg L -1 ) to water-stressed plants further enhanced the activities of antioxidant enzymes, CA activity, and Pro and GB content. Nano-TiO 2 induced increase in ROS, CA, Pro and GB resulted in a decrease in ROS level, electrolyte leakage and lipid peroxidation leading to increase in LRWC, Chl content, NR activity and growth of the plants. Furthermore, an additional increase in the level of nano-TiO 2 to 25 mg L -1 could not alleviate water stress and showed adverse effects.
The Improvement of Maize (Zea mays L.) for Drought Stress Tolerance

Maize is one of the important cereals in the world after rice and wheat. It is grown for grain as well as fodder in tropical, sub-tropical and temperate regions of the World. The reduction of maize productivity under drought stress conditions depends on different factors such as plant development stage, drought intensity and duration of water deficit, and varietal sensitivity to drought stress . Drought stress is one of the major constraints to agriculture where limited water availability for crop irrigation causes a reduction in carbon fixation by the photosynthetic apparatus that result in net yield losses. Plant breeders desire novel and more accurate tools to fast-track breeding programs required for the increasing demands of food and fodder because farmers are facing a changing climate in which drought stress has major impacts on crop damages globally. Crop loss, because of drought stress, has a huge impact on agriculture industry and the economy. Therefore, to prevent economic loss and to make the crop plants more tolerant to drought stress, a variety of approaches such as conventional breeding and molecular/ genetic engineering are being practiced.
The non-canonical small heat shock protein HSP-17 from C. elegans is a selective protein aggregase

Small heat shock proteins (sHsps) are conserved, ubiquitous members of the proteostasis network. Canonically, they act as “holdases” and buffer unfolded or misfolded proteins against aggregation in an ATP-independent manner. While bacteria and yeast each have only two sHsps in their genomes, this number is higher in metazoan genomes, suggesting a spatiotemporal and functional specialization in higher eukaryotes. Here, using recombinantly expressed and purified proteins, static light-scattering analysis and disaggregation assays, we report that the non-canonical sHsp HSP-17 of Caenorhabditis elegans facilitates aggregation of model substrates such as malate dehydrogenase (MDH) and inhibits disaggregation of luciferase in vitro . Experiments with fluorescently tagged HSP-17 under the control of its endogenous promoter revealed that HSP-17 is expressed in the digestive and excretory organs where its overexpression promotes the aggregation of polyQ proteins and of the endogenous kinase KIN-19. Systemic depletion of hsp-17 shortens and and activities on selective protein aggregase physiological in and
High Resolution Seismic Imaging of Very Shallow Highly Contrasted Structures

maging very shallow highly contrasted structures with seismic methods stands for a difficult task because of the interpretation complexity. In particular, body waves carry the main part of information about the structure while they are much less energetic than surface waves which dominate the data. In this study we assess the performances of two different imaging techniques for the characterization of concrete foundations. Relying on 2D-synthetic examples, we show Full Waveform Inversion behaves well on structures with low properties contrasts but fail to image realistic contrasts of concrete in soft sediments. We also apply elastic reverse time migration and conclude on its ability to characterize the geometry of the upper part of foundations
R g Engaged in Phagocytosis Mediated by Fc Lyn and Syk Kinases Are Sequentially

Recent data indicate that phagocytosis mediated by Fc (cid:1) Rs is controlled by the Src and Syk families of protein tyrosine kinases. In this study, we demonstrate a sequential involvement of Lyn and Syk in the phagocytosis of IgG-coated particles. The particles isolated at the stage of their binding to Fc (cid:1) Rs (4°C) were accompanied by high amounts of Lyn, in addition to the signaling (cid:1) -chain of Fc (cid:1) Rs. Simultaneously, the particle binding induced rapid tyrosine phosphorylation of numerous proteins. During synchro-nized internalization of the particles induced by shifting the cell to 37°C, Syk kinase and Src homology 2-containing tyrosine phosphatase-1 (SHP-1) were associated with the formed phagosomes. At this step, most of the proteins were dephosphorylated, although some underwent further tyrosine phosphorylation. Quantitative immunoelectron microscopy studies conﬁrmed that Lyn accumulated under the plasma membrane beneath the bound particles. High amounts of the (cid:1) -chain and tyrosine-phosphorylated proteins were also observed under the bound particles. When the particles were internalized, the (cid:1) -chain was still detected in the region of the phagosomes, while amounts of Lyn were markedly reduced. In contrast, the vicinity of the phagosomes was heavily decorated with anti-Syk and anti-SHP-1 Abs. The local level of protein tyrosine phosphorylation was reduced. The data indicate that the accumulation of Lyn during the binding of IgG-coated particles to Fc (cid:1) Rs correlated with strong tyrosine phosphorylation of numerous proteins, suggesting an initiating role for Lyn in protein phosphorylation at the onset of the phagocytosis. Syk kinase and SHP-1 phosphatase are mainly engaged at
Energy and water balance of a treatment wetland under mediterranean climatic conditions

During 2013 – 2014 period, the surface renewal (SR) – energy balance (EB) method combined with the water balance approach was adopted to de ﬁ ne the energy and water budget of a treatment wetland (TW) in Eastern Sicily (Italy). The main objective was to evaluate the di ﬀ erences between energy and water balances and un- derstand the underlining mechanisms of environmental control. Results shown that the seasonal variation in evapotranspiration (ET) had similar trend as the seasonal variation of leaf area index (LAI) and net radiation (R n ). During the study period, the daily rate of ET by EB ranged from − 0.1mmd − 1 to 9.3mmd − 1 , with a total ET of about 1837mm in 17months. The crop coe ﬃ cient K c (e.g. obtained using the FAO-56 mean K c approach) for the TW ranged from 0.5 to 2.4, with a mean annual value of 1.24. On a 10-day mean basis, ET ﬂ uxes from the TW water balance were 15% higher than ET by EB.
Optimisation de la phytoextraction : caractérisation et sélection de bactéries PGPB associées à une plante hyperaccumulatrice de Zn et Cd : Arabidopsis halleri

phytoextraction bactéries PGPB associées plante hyperaccumulatrice Résumé Les phytotechnologies consistent en l’utilisation de plantes qui, par leur association avec la microflore du sol, ont la capacité de dépolluer les sites contaminés (eau, sol, sédiments). Parmi ces technologies, la phytoextraction, basée sur l’utilisation de plantes hyperaccumulatrices telle qu’ Arabidopsis halleri , semble être une option pour décontaminer les sols pollués en éléments traces métalliques. Une des voies d’amélioration de la phytoextraction d’ A. halleri serait l’inoculation du système racinaire de la plante par des bactéries capables de stimuler la croissance végétale (PGPB pour Plant Growth Promoting Bacteria). Dans cette étude, nous proposons un protocole d’échantillonnage des différentes fr actions de sol autour de la racine : sols global, rhizosphérique et rhizoplan. Les bactéries cultivables, collectées sur un sol industriel contaminé en Zn et Cd (Auby, France), ont été isolées et analysées pour leurs caractéristiques PGPB : (ACC) Abstract Phytotechnologies are microbial-assisted techniques that use living plants for the treatment of contaminated sites. Among these, phytoextraction based on hyperaccumulator plants such as Arabidopsis halleri , may be an option to remove trace elements in soil. One method to optimize Arabidopsis halleri phytoextraction is the inoculation of plant roots by plant growth promoting bacteria (PGPB). In this study, we proposed a protocol for sampling bacteria in three different soil fractions neared root system of Arabidopsis halleri : global, rhizospheric and rhizoplan. The cultivable bacteria were isolated for a Zn and Cd contaminated industrial soil (Auby, France) and were isolated and characterized for their PGPB traits : 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, siderophores and Indol-Acetic-Acid (IAA) productions). Bacteria answering positively to the three PGPB tests have been preferentially selected to be inoculated to Arabidopsis halleri root system. Bacteria effects on plant biomass and/or accumulating yield will be presented. 30°C in the dark with a 120 rpm shaking for 5 d. Culture supernatants were recovered after centrifugation at 6,000 g for 10 min. One ml of supernatant was mixed with 150 µl of Salkowski’s reagent R1 (12 g FeCl 3 in 429 ml H 2 SO 4 18M). After a 25°C incubation in the dark for 20 min, absorbance at 535 nm was measured.
Posttranslational modification of the NADP-malic enzyme involved in C 4 photosynthesis 2 fine-tunes the enzymatic activity during the day 3

41 Evolution of the C 4 photosynthetic pathway involved in some cases recruitment of 42 housekeeping proteins through gene duplication and their further neofunctionalization. 43 NADP-malic enzyme (ME), the most widespread C 4 decarboxylase, has increased its catalytic 44 efficiency and acquired regulatory properties that allowed it to participate in the C 4 pathway. 45 Here, we show that regulation of maize C 4 -NADP-ME activity is much more elaborated than 46 until now indicated. Using mass spectrometry, we identified phosphorylation of the serine 419 47 (S419) of C 4 -NADP-ME in protein extracts of maize leaves. The phosphorylation event 48 increases after the light turns on, with a peak at ZT2. Phosphorylation of ZmC 4 -NADP-ME 49 drastically decreases its activity as shown by the low residual activity of the recombinant 50 phosphomimetic mutant. Analysis of the crystal structure of C 4 -NADP-ME indicated that 51 S419 is involved in the binding of NADP at the active site. Molecular dynamics simulations 52 and effective binding energy computations indicate a less favorable binding of the cofactor 53 NADP in the phosphomimetic and the phosphorylated variants. We propose that 54 phosphorylation of ZmC 4 -NADP-ME at S419 during the first hours in the light is a cellular 55 mechanism to fine-tune the enzymatic activity to coordinate the carbon concentration 56 mechanism with the CO 2 fixation rate, most probably to avoid CO 2 leakiness from bundle 57 sheath cells. digestion, trypsin; tripleTOF 6600; special factors,
Impact and Interaction of On Load Tap Changer and Smart PV Inverter

-With great increasing concern about fossil fuel shortage the incentive motivating to improve alternative energy sources. Between renewable energy sources used, energy through photovoltaic is common. Lately, pv system could be widely used as a viable replacement to traditional sources.Unfortunately, PV systems create several challenges to the operation and voltage control to the utilities, So Voltage regulation is needed. Voltage regulation control methods have been proposed in this study considering the inverter of the pv system and OLTC in ieee 15_bus system to regulate the voltage during the studied period. An14_hour simulation is performed considering PV and load variation. The simulation results reveal that the use of smart PV inverter with OLTC may not be the best way to regulate the voltage and there is a need to make coordination between them.