Miracle-dz/UniFAR-FTUs huggingface dataset:
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF TRICLOSAN IN DENTAL FORMULATIONS

The present study is a simple rapid, sensitive, selective and reproducible RP-HPLC method has been developed for the estimation of Triclosan in dental formulations. In present work a simple, sensitive and specific method (RPHPLC assay, stability indicating RP-HPLC) has been developed by using a phenomenex , BDS C18, column having 5 μm particle size and 150 mm x 4.6 mm in length and gradient mode, with mobile phase containing acetic acid buffer (pH 2.5, adjusted with formic acid) and acetonitrile in the ratio of 30:70. The flow rate was 1.2mL/min and effluents were monitored by PDA detector at 281nm. Injection volume was 20 μL. The method is linear (1-50μg/mL). The regression equation was y = 7311x-2307 (r 2 =0.997). The method was validated for linearity, precision, accuracy, ruggedness, and forced degradation studies were performed. Estimation of triclosan was done in 3 dental formulations. Recoveries from formulations were between 98% and 102%. Limit of detection and limit of quantification was 0.101433 and 0. 3333μg/mL respectively. All the validation parametes are within acceptance range. INTRODUCTION: Triclosan (5-chloro-2-(2,4dichlorophenoxy)phenol) (Figure 1) is a strong broad-spectrum anti-microbial agent used in many consumer products, such as anti-bacterial soaps, toothpastes, cosmetics, clothing, kitchenware and toys 1 . Triclosan is 2 . It is a white solid at standard temperature and pressure, with a boiling point in the range of 280-290 °C and a melting point in the range of 56-58 °C. Triclosan has a low partition coefficient (log Po/w= 4.7) 3 . The partition coefficient is a ratio of solubility between two liquids, typically octanol and water. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.5(9). 3810-16 Article can be accessed online on: www.ijpsr. com DOI link: http://dx.doi. org/10.13040/IJPSR.0975-8232.5(9). 3810-16 Triclosan discharged into surface waters through wastewater effluents react photochemically to form polychlorinated dibenzo-p-dioxins 4 . From the literature review it is known that various methods have been reported. A gas chromatography-tandem mass spectrometry (GC-MS/MS) 5 has been developed for the simultaneous analysis of different classes of preservatives like benzoates, bronidox, 2phenoxyethanol, parabens, BHA, BHT and triclosan in cosmetic products. FIG 1: STRUCTURE OF TRICLOSAN Liquid chromatography (HPLC)/GC-MS method for triclosan in personal health care products 6-9 , human milk 10 , GC–MS analysis in Indian rivers 11 ,
Manipulation of a spider peptide toxin alters its affinity for lipid bilayers and potency and selectivity for voltage-gated sodium channel subtype 1.7

Huwentoxin-IV (HwTx-IV) is a gating modifier peptide toxin from spiders that has weak affinity for the lipid bilayer. As some gating modifier toxins have affinity for model lipid bilayers, a tripartite relationship among gating modifier toxins, voltage-gated ion channels, and the lipid membrane surrounding the channels has been proposed. We previously designed an HwTx-IV analogue (gHwTx-IV) with reduced negative charge and increased hydrophobic surface profile, which displays increased lipid bilayer affinity and in vitro activity at the voltage-gated sodium channel subtype 1.7 (NaV1.7), a channel targeted in pain management. Here, we show that replacements of the positively-charged residues that contribute to the activity of the peptide can improve gHwTx-IV's potency and selectivity for NaV1.7. Using HwTx-IV, gHwTx-IV, [R26A]gHwTx-IV, [K27A]gHwTx-IV, and [R29A]gHwTx-IV variants, we examined their potency and selectivity at human NaV1.7 and their affinity for the lipid bilayer. [R26A]gHwTx-IV consistently displayed the most improved potency and selectivity for NaV1.7, examined alongside off-target NaVs, compared with HwTx-IV and gHwTx-IV. The lipid affinity of each of the three novel analogues was weaker than that of gHwTx-IV, but stronger than that of HwTx-IV, suggesting a possible relationship between in vitro potency at NaV1.7 and affinity for lipid bilayers. In a murine NaV1.7 engagement model, [R26A]gHwTx-IV exhibited an efficacy comparable with that of native HwTx-IV. In summary, this study reports the development of an HwTx-IV analogue with improved in vitro selectivity for the pain target NaV1.7 and with an in vivo efficacy similar to that of native HwTx-IV.
Longitudinal investigation of the role of cognitive reserve in the evolution of dementia in outpatients prescribed AChEI

ABSTRACT Aim: The role of cognitive reserve (CR) in modulating dementia has been broadly investigated. We aimed to evaluate the long-term effects of CR on cognitive functions in outpatients newly treated with acetylcholinesterase inhibitors. Method: Fifty older adults with dementia (age 80 ± 6.4 years) were followed up over 27 months. CR was assessed with the Cognitive Reserve Index questionnaire (CRIq), which provides a Total CR index and three proxy measures: Education, Working Activity and Leisure Time. The association between CR and cognition, evaluated by the Mini-Mental State Examination (MMSE), was tested through linear mixed models. Results: The cognitive profile of High CR individuals (n = 16) was more fluctuating than that of patients with Low CR (n = 34) up to 15 months of treatment, showingan alternation of improvements and worsening. At linear mixed models, CRIq Total score was significantly associated with MMSE over the follow-up either when considered as continuous (β = 0.13 [95%CI:0.07–0.19], p < .001, per each 1-unit increase) orcategorical variable (β = 3.62 [95%CI:1.77–5.47], p = .002, High vs Low CR). Among the CR domains, higher CRIq Leisure-time scores were significantly associated with higher MMSE during the follow-up (β = 0.05 [95%CI:0.02–0.09], p = .009, per each 1-unit increase). Conclusion: The study indicates that higher CR, and especially Leisure Time-related CR, was associated with better cognitive performance in older outpatients with dementia treated with AChEI for 27 months. These findings suggest that Leisure Time-related CR could influence the evolution of dementia, and support the need of further investigations to verify the potential usefulness of interventions enhancing such domain even in advanced age.
Pansharpening Based Multispectral Image Enhancement under Dual Transform

: The paper presents satellite image enhancement based on SWT, NSCT with Gabor based pixel level fusion for Pansharpening. SWT decompose the image into finer and coarser details and these details will be decomposed into different orientation edges using NSCT. NSCT is very efficient in representing the directional information and capturing intrinsic geometrical structures of the objects. It has characteristics of high resolution, shift-invariance, and high directionality. An Image fusion involves Gabor filter to fuse finest details obtained from NSCT It enhances the spatial information of multi spectral images, Gabor is useful to discriminate and characterize the texture of an image through frequency and orientation representation. All subbands are fused by pixel level fusion with maximum rule to achieve better accuracy. An integration of high spatial resolution extracted from PAN images into the high spectral resolution of MS images generates both high spatial and spectral resolution pansharpened image. Here, a given number of decomposition levels are used for multispectral images while a higher number of decomposition levels are used for Pan Images relatively to the ratio of the Pan Pixel size to the MS pixel size. This preserves both spectral and spatial qualities while decreasing computation time. By applying upsampling after NSCT, structures and detail information of the MS images are more likely to be preserved. Hence, pansharpening is done by fusing it with detail information provided by the Pan image at the same fine level. The system simulated result shows that used method provides better resolution in these images rather than prior approaches and it also measured the performance parameters such as correlation, PSNR, PSD and standard deviation.
Comparison of glucose variability in patients with type 2 diabetes administrated glargine with needle-free jet injector and conventional insulin pen

ABSTRACT Background The effects of insulin delivered by needle-free jet injector on glycemic variations (GV) in patients with type 2 diabetes (T2D) which remains largely unknown. Research design and methods We aimed to compare the glucose variability in Chinese T2D patients administrated glargine 100 U/mL (Gla-100) with a needle-free jet injector and conventional insulin pen. This was a self-comparative, observational study of 26 patients who were subjected to a flash glucose monitoring system (FGM) for 14 consecutive days. During the study period, all subjects received Gla-100 daily before breakfast using a conventional insulin pen, with the exception of day 3 and day 7, when insulin was delivered by needle-free jet injection. We analyzed FGM data of day 3, day 5, day 7, and day 9 at the endpoint. Results There were no differences in the glycemic variability between the jet injector group and the conventional pen group. However, patients with needle-free jet injection had a lower 24 hr mean glucose (MG) and lower incremental area under the curve (AUC) of 1 hr, 12 hr, and 24 hr, compared to the conventional pen group (p = 0.001). Conclusions Gla-100 delivered by needle-free jet injection potentially lowered MG in patients with T2D in the Chinese population.
Epoxy Coatings Based on Modified Vegetable Oils for Wood Surface Protection against Fungal Degradation.

The study describes the curing and thermal behaviour of a new castor oil maleic anhydride adduct/epoxy oils/5-Bromosalicylic acid coatings and their composites with wood. The epoxidized oils were flax and hemp. The kinetic parameters of the curing and thermal degradation processes were calculated. The resistance of the coated wood surfaces against Cladosporium cladosporioides, Aspergillus brasiliensis and Penicillium chrysogenum was tested. Colour changes, FT-IR and SEM were conducted before and after fungal attack. The decay resistance and colour change of raw wood and wood treated samples against fungi was tested. Based on the colour changes and according to ASTM D 2017, the decay resistance rating for covered samples was considered as "highly resistant". Chemical resistance and coating performance tests were also undertaken. The obtained results recommend the described materials for applications in wood protective coatings.
Context effects on probability estimation

Many decisions rely on how we evaluate potential outcomes and estimate their corresponding probabilities of occurrence. Outcome evaluation is subjective because it requires consulting internal preferences and is sensitive to context. In contrast, probability estimation requires extracting statistics from the environment and therefore imposes unique challenges to the decision maker. Here, we show that probability estimation, like outcome evaluation, is subject to context effects that bias probability estimates away from other events present in the same context. However, unlike valuation, these context effects appeared to be scaled by estimated uncertainty, which is largest at intermediate probabilities. Blood-oxygen-level-dependent (BOLD) imaging showed that patterns of multivoxel activity in the dorsal anterior cingulate cortex (dACC), ventromedial prefrontal cortex (VMPFC), and intraparietal sulcus (IPS) predicted individual differences in context effects on probability estimates. These results establish VMPFC as the neurocomputational substrate shared between valuation and probability estimation and highlight the additional involvement of dACC and IPS that can be uniquely attributed to probability estimation. Because probability estimation is a required component of computational accounts from sensory inference to higher cognition, the context effects found here may affect a wide array of cognitive computations.
Potential Factors Influencing Repeated SARS Outbreaks in China

Within last 17 years two widespread epidemics of severe acute respiratory syndrome (SARS) occurred in China, which were caused by related coronaviruses (CoVs): SARS-CoV and SARS-CoV-2. Although the origin(s) of these viruses are still unknown and their occurrences in nature are mysterious, some general patterns of their pathogenesis and epidemics are noticeable. Both viruses utilize the same receptor—angiotensin-converting enzyme 2 (ACE2)—for invading human bodies. Both epidemics occurred in cold dry winter seasons celebrated with major holidays, and started in regions where dietary consumption of wildlife is a fashion. Thus, if bats were the natural hosts of SARS-CoVs, cold temperature and low humidity in these times might provide conducive environmental conditions for prolonged viral survival in these regions concentrated with bats. The widespread existence of these bat-carried or -released viruses might have an easier time in breaking through human defenses when harsh winter makes human bodies more vulnerable. Once succeeding in making some initial human infections, spreading of the disease was made convenient with increased social gathering and holiday travel. These natural and social factors influenced the general progression and trajectory of the SARS epidemiology. However, some unique factors might also contribute to the origination of SARS in Wuhan. These factors are discussed in different scenarios in order to promote more research for achieving final validation.
Abdominal Compartment Syndrome: diagnostic evaluation and possible treatment.

BACKGROUND Abdominal compartment syndrome (ACS) is a clinical condition caused by an increase in intra-abdominal pressure. The incidence is variable, based on the type of patients taken into consideration, increasing exponentially in critical conditions, such as traumatized, burned patients in shock. The syndrome can also follow surgical procedures like transplantation of abdominal organs, for example configuring rare pictures such as RACS (post-transplant kidney syndrome). In most cases the symptoms are non-specific and varied according to the different etiology of the ACS, therefore the diagnosis may not be immediate. AIM The aim of this work is to evaluate the best therapeutic approach based on the evidence in the literature. RESULTS An early diagnosis, which can also identify the stages of intra-abdominal hypertension, is necessary to ensure the survival of the patient, implementing an integrated multidisciplinary treatment, expecially in the early stages of the development of the ACS. CONCLUSIONS The definitive treatment is surgical and is based on the decompression of the abdomen.
Estimating and accounting for genotyping errors in RAD-seq experiments

In non-model organisms, evolutionary questions are frequently addressed using reduced representation sequencing techniques due to their low cost, ease of use, and because they do not require genomic resources such as a reference genome. However, evidence is accumulating that such techniques may be affected by specific biases, questioning the accuracy of obtained genotypes, and as a consequence, their usefulness in evolutionary studies. Here we introduce three strategies to estimate genotyping error rates from such data: through the comparison to high quality genotypes obtained with a different technique, from individual replicates, or from a population sample when assuming Hardy-Weinberg equilibrium. Applying these strategies to data obtained with Restriction site Associated DNA sequencing (RAD-seq), arguably the most popular reduced representation sequencing technique, revealed per-allele genotyping error rates that were much higher than sequencing error rates, particularly at heterozygous sites that were wrongly inferred as homozygous. As we exemplify through the inference of genome-wide and local ancestry of well characterized hybrids of two Eurasian poplar (Populus) species, such high error rates may lead to wrong biological conclusions. By properly accounting for these error rates in downstream analyses, either by incorporating genotyping errors directly or by recalibrating genotype likelihoods, we were nevertheless able to use the RAD-seq data to support biologically meaningful and robust inferences of ancestry among Populus hybrids. Based on these findings, we strongly recommend carefully assessing genotyping error rates in reduced representation sequencing experiments, and to properly account for these in downstream analyses, for instance using the tools presented here.
Probing the Environment of Emerin by Enhanced Ascorbate Peroxidase 2 (APEX2)-Mediated Proximity Labeling

Emerin is one of the best characterized proteins of the inner nuclear membrane, but can also occur at the level of the endoplasmic reticulum. We now use enhanced ascorbate peroxidase 2 (APEX2) to probe the environment of emerin. APEX2 can be used as a genetic tag that produces short-lived yet highly reactive biotin species, allowing the modification of proteins that interact with or are in very close proximity to the tagged protein. Biotinylated proteins can be isolated using immobilized streptavidin and analyzed by mass spectrometry. As an alternative to the standard approach with a genetic fusion of APEX2 to emerin, we also used RAPIDS (rapamycin- and APEX-dependent identification of proteins by SILAC), a method with improved specificity, where the peroxidase interacts with the protein of interest (i.e., emerin) only upon addition of rapamycin to the cells. We compare these different approaches, which, together, identify well-known interaction partners of emerin like lamin A and the lamina associated polypeptide 1 (LAP1), as well as novel proximity partners.
Immune cell infiltration-based signature for prognosis and immunogenomic analysis in breast cancer

Breast cancer is one of the most human malignant diseases and the leading cause of cancer-related death in the world. However, the prognostic and therapeutic benefits of breast cancer patients cannot be predicted accurately by the current stratifying system. In this study, an immune-related prognostic score was established in 22 breast cancer cohorts with a total of 6415 samples. An extensive immunogenomic analysis was conducted to explore the relationships between immune score, prognostic significance, infiltrating immune cells, cancer genotypes and potential immune escape mechanisms. Our analysis revealed that this immune score was a promising biomarker for estimating overall survival in breast cancer. This immune score was associated with important immunophenotypic factors, such as immune escape and mutation load. Further analysis revealed that patients with high immune scores exhibited therapeutic benefits from chemotherapy and immunotherapy. Based on these results, we can conclude that this immune score may be a useful tool for overall survival prediction and treatment guidance for patients with breast cancer.
Authentication of Smartphone Users Using Behavioral Biometrics And OPass Technique

: Smartphones and tablets have become ubiquitous in our daily lives. Smartphones, in particular, have become more than personal assistants. These devices have provided new avenues for consumers to play, work and socialize whenever and wherever they want. Password-based user authentication can resist brute force and dictionary attacks if users select strong passwords to provide sufficient entropy. However, password-based user authentication has a major problem that humans are not experts in memorizing text strings. oPass only requires each participating website possesses a unique phone number
3,4-Dihydroxybenzalacetone (DBL) Prevents Aging-Induced Myocardial Changes in Senescence-Accelerated Mouse-Prone 8 (SAMP8) Mice

Aging is a predominant risk factor for the development and progression of cardiovascular complications. Physiologically and anatomically, the heart undergoes numerous changes that result in poor cardiac function in the elderly population. Recently, several studies have provided promising results, confirming the ability of the senescence-accelerated mouse-prone 8 (SAMP8) model to accurately model age-related cardiovascular alterations. In this study, using a murine model of senescence, SAMP8, we aimed to investigate the effect of 3,4-dihydroxybenzalacetone (DBL), a catechol-containing phenylpropanoid derivative isolated from Inonotus obliquus (Chaga), on cardiac aging. DBL was administered at the doses of 10 mg/kg and 20 mg/kg by oral gavage to SAMP8 mice to examine aging-mediated cardiac changes, such as oxidative DNA damage, oxygen radical antioxidant capacity (ORAC) value, fibrosis, inflammation, and apoptosis. The treatment with DBL at both doses significantly reduced aging-mediated oxidative DNA damage, and simultaneously increased the ORAC value in the SAMP8 assay. Cardiac fibrosis was assessed with Azan-Mallory staining, and the number of cardiac remodeling markers was found to be significantly reduced after the treatment with DBL. We also observed a decrease in cardiomyocyte apoptosis as measured by the terminal transferase-mediated dUTP nick end labeling (TUNEL) staining method and the caspase-3 levels in SAMP8 mice compared with senescence-resistant control (SAMR1) mice. The findings from this study suggest that DBL has a potentially beneficial effect on aging-mediated myocardial alterations. Further studies are warranted to confirm the promising potential of this catechol compound against aging-associated myocardial dysfunction.
Prostate cancer screening knowledge, attitudes, and beliefs among men in Bamenda, Cameroon

In Cameroon, few population-based studies have examined factors associated with screening for prostate cancer. This cross-sectional study aimed to investigate prostate cancer-related knowledge, assess rates of prostate cancer screening, and explore factors influencing screening in Bamenda. Questionnaires were administered to a cluster random sample of 556 men. Logistic regression was used to estimate the effects of the factors on screening. A medium level of knowledge was found among 55.2% of participants regarding prostate cancer risk factors while the screening rate for that affection was 8.1% among the study population. The adjusted odds ratio (OR) and 95% confidence interval (CI) for prostate cancer screening among men who are 65 years-old and older as opposed to men aged 40 to 44 years-old was 4.4 [1.1 17.6]. Furthermore, 6.4 [1.9 – 20.9] was the adjusted OR and 95% CI for screening among men with income > 150,000.00 Francs relative to those with income < 50,000.00 Francs. The adjusted OR and 95% CI was 14.8 [6.6 – 33.4] for screening among men aware of screening tests relative to those not aware. Age, income, and awareness were the factors associated with prostate cancer screening among men in Bamenda where screening rate was low and knowledge level was average.
Feature Subset Selection with Fast Algorithm Implementation

-- The Title “Feature Subset Selection with FAST Algorithm Implementation” has intended to show the things occurred in between the searches happened in the place of client and server. The users clearly know about the process of how to sending a request for the particular thing, and how to get a response for that request or how the system shows the results explicitly. But no one knows about the internal process of searching records from a large database. This system clearly shows how an internal process of the searching process works. In text classification, the dimensionality of the feature vector is usually huge to manage. The Problems need to be handled are as follows: a) the current problem of the existing feature clustering methods b) The desired number of extracted features has to be specified in advance c) When calculating similarities, the variance of the underlying cluster is not considered d) How to reduce the dimensionality of feature vectors for text classification and run faster. These Problems are handled by means of applying the FAST Algorithm in hands with Association Rule Mining.