Miracle-dz/UniFAR-FTUs huggingface dataset:
The Impacts of Human-Cobot Collaboration on Perceived Cognitive Load and Usability during an Industrial Task: An Exploratory Experiment

OCCUPATIONAL APPLICATIONS Since cobots (collaborative robots) are increasingly being introduced in industrial environments, being aware of their potential positive and negative impacts on human collaborators is essential. This study guides occupational health workers by identifying the potential gains (reduced perceived time demand, number of gestures and number of errors) and concerns (the cobot takes a long time to perceive its environment, which leads to an increased completion time) associated with working with cobots. In our study, the collaboration between human and cobot during an assembly task did not negatively impact perceived cognitive load, increased completion time (but decreased perceived time demand), and decreased the number of gestures performed by participants and the number of errors made. Thus, performing the task in collaboration with a cobot improved the user’s experience and performance, except for completion time, which increased. This study opens avenues to investigate how to improve cobots to ensure the usability of the human-machine system at work. TECHNICAL ABSTRACT Background Industry 4.0 implements smart technologies to increase productivity and to decrease the associated risks. Using cobots is considered by industry as a potential means to reduce physical constraints and improve performance without having to replace the human factor. Research has yet to prove these benefits on humans. Purpose The goal of this study was to determine if working with a cobot improved perceived cognitive load of an operator and the usability of the system. Methods Participants replicated three construction models using Duplos. Approximatively half of our participants (n = 32) accomplished the task alone and the other half accomplished the same task with a cobot (n = 22). We then used the NASA-TLX to measure workload and, through a sub-dimension, perceived cognitive load. Completion time (for each model), number of errors (placing and replacing a piece), and the number of gestures (movements of the upper limbs) were also measured. Results Collaboration with a cobot led to significantly fewer gestures (51 vs. 74), fewer errors (2 vs. 8), and reduced perceived time demand but increased completion time (136 vs. 55 s). Perceived cognitive load was not impacted by the cobot (36 vs. 37). Conclusion We conclude that collaboration with a cobot adapting to human variability is possible, and that it could lead to better performance and could improve certain dimensions of system usability.
Influence of Incorporating Zirconium- and Barium-based Radiopaque Filler Into Experimental and Commercial Infiltrants.

OBJECTIVES To evaluate how adding different concentrations of particles (barium or zirconium oxide 25%/45% by weight) to a commercial infiltrant (Icon) and an experimental infiltrant influences cohesive strength (CS), degree of conversion (DC), water sorption (WS), solubility (SL), radiopacity, and penetration depth. METHODS AND MATERIALS Microtensile CS (n=10) was evaluated using a universal testing machine. DC (n=5) was evaluated in a Fourier-transform infrared spectrometer. Polymerized samples were dissected, weighed, and stored to obtain the final mass for WS and SL tests (n=10). Radiopacity analysis (n=5) was performed using a digital radiography system. Penetration depth analysis (n=5) was performed by confocal laser scanning microscopy. Analyses were performed using the R program, with a significance level of 5%, except for the penetration depth analyses, which were evaluated only qualitatively. RESULTS The groups with 45% zirconium showed greater CS values, regardless of the infiltrant. Among the groups with no particle addition, those of the experimental infiltrant presented higher DC than those of Icon. The experimental infiltrant presented lower WS than Icon. All groups had SL below the ISO recommended levels. Radiopacity higher than 2.24 mmAl (enamel radiopacity) was observed only in the groups with 45% zirconium. All the groups achieved similar penetration depth, but the groups containing experimental infiltrant appear to have had longer tag extensions. CONCLUSIONS Addition of 45% of zirconium presented good results for CS and WS, as well as SL below the ISO recommended standard, adequate radiopacity, and penetration depth similar to the other groups.
Real-world statistics at two timescales and a mechanism for infant learning of object names

Significance Infants learn mappings between heard names and seen things before their first birthday and before they produce spoken language. Two challenges to explaining this early learning are the immaturity of infant memory systems and the infrequency of any individual object name in the heard language input. We quantified the frequency of visual referents, heard names, and the cooccurrences of referents and names in infant everyday experiences. We discovered statistical patterns at two timescales that align with a cortical mechanism of associative memory formation that supports the rapid formation of durable associative memories from very few experienced cooccurrences.
The impact of life stress, psychological resources, and proactive behaviors on quality of life among people living with HIV

Abstract Objectives Quality of life (QoL) is an important consideration for people living with HIV (PWH). We investigated the relationship between stress, psychological resources, and proactive behaviors, on QoL (conceptualized as life satisfaction, successful aging, and depressive symptoms) by testing the hypotheses: (1) greater life stress (stress and functional impairment) is associated with poorer QoL; (2) resources (mastery, resilience, and social support) are associated with better QoL, beyond the influence of stress; and (3) proactive behaviors (medication management and leisure activities) mediate the relationship between resources and QoL. Methods Secondary analyses were performed (N = 128 PWH). Participants’ mean age was 52.3, 83.6% were male, and 53.9 identified as white. Multivariate regressions were performed within the context of path analyses. Results In series 1, greater stress was associated with poorer life satisfaction (p < 0.001), lower self-rated successful aging (p < 0.001), and greater depression (p < 0.001). Functional impairment was associated with lower successful aging (p = 0.017) and greater depression (p = 0.001). In series 2, which accounted for mastery, resilience, social support, as well as demographic covariates, mastery was associated with greater life satisfaction (p = 0.038). In series 3, stress, functional impairment, leisure activities, and ART management were added to the model and social support was associated with engagement in leisure activities (p < 0.001), which was associated with better successful aging (p = 0.006). Fit indices suggested adequate relative fit. In bootstrapped analyses of indirect effects, social support was indirectly associated with successful aging through leisure activities (p = 0.020). Conclusions QoL, as captured by self-rated successful aging, is threatened by stress but positively influenced by social support and engaging in leisure activities. Findings support a model of proactive successful aging for PWH.
Tuning levels of low-complexity domain interactions to modulate endogenous oncogenic transcription

Gene activation by mammalian transcription factors (TFs) requires dynamic, multivalent, and selective interactions of their intrinsically disordered low-complexity domains (LCDs), but how such interactions mediate transcription remains unclear. It has been proposed that extensive LCD-LCD interactions culminating in liquid-liquid phase separation (LLPS) of TFs is the dominant mechanism underlying transactivation. Here, we investigated how tuning the amount and localization of LCD-LCD interactions in vivo affects transcription of endogenous human genes. Quantitative single-cell and single-molecule imaging reveals that the oncogenic TF EWS/FLI1 requires a finely tuned range of LCD-LCD interactions to efficiently activate target genes. Modest or more dramatic increases in LCD-LCD interactions toward putative LLPS repress EWS/FLI1-driven transcription in patient cells. Likewise, ectopically creating LCD-LCD interactions to sequester EWS/FLI1 into a bona fide LLPS compartment, the nucleolus, inhibits EWS/FLI1-driven transcription and oncogenic transformation. Our findings reveal fundamental principles underlying LCD-mediated transcription and suggest mislocalizing specific LCD-LCD interactions as a novel therapeutic strategy for targeting disease-causing TFs.
Biomechanical properties of masticatory balance in cases with RPDs—The influence of preferred and nonpreferred chewing side: A pilot study

Removable partial dentures (RPDs) are inserted with the aim to restore masticatory function. There is however inconsistent evidence supporting the alleged improvements, posterior occlusal contacts being one of the decisive factors. We hypothesized that the distribution of abutment teeth in RPDs influences masticatory performance and functional parameters. To evaluate the masticatory performance and functional parameters in patients with a RPD using a single mathematical parameter (tilting index [TI]) for both jaws that predicts biomechanical behavior on the basis of the distribution of abutment teeth.
Long intergenic non-protein coding RNA 00707 regulates chondrocyte apoptosis and proliferation in osteoarthritis by serving as a sponge for microRNA-199-3p

ABSTRACT It is known that long intergenic non-protein coding RNA 00707 (LINC00707) promotes lipopolysaccharide (LPS)-injury and microRNA-199-3p (miR-199-3p) regulates chondrocyte proliferation and apoptosis. Both processes participate in osteoarthritis (OA). We predicted that LINC00707 and miR-199-3p may interact with each other. Therefore, LINC00707 and miR-199-3p may interact with each other to participate in OA. In this study, the expression of LINC00707 and miR-199-3p in both OA and normal articular cartilage tissues was analyzed using RT-qPCR. The subcellular location of LINC00707 and its direct interaction with miR-199-3p were explored by nuclear fractionation assay, RNA pull-down assay and Luciferase reporter assay, respectively. The role of LINC00707 and miR-199-3p in regulating the expression of each other was analyzed using an overexpression assay, followed by RT-qPCR. The role of LINC00707 and miR-199-3p in regulating OA chondrocyte proliferation and apoptosis was analyzed by BrdU assay and cell apoptosis assay, respectively. OA tissues exhibited increased expression of LINC00707 and decreased expression of miR-199-3p. LINC00707 directly interacted with miR-199-3p in cytoplasm. However, LINC00707 and miR-199-3p overexpression failed to affect each other’s expression. LPS treatment increased LINC00707 expression and decreased miR-199-3p expression in OA chondrocyte. LINC00707 overexpression increased the apoptosis of OA chondrocytes induced by LPS and suppressed the proliferation of OA chondrocytes. Moreover, LINC00707 suppressed the role of miR-199-3p in enhancing cell proliferation and suppressing cell apoptosis. In conclusion, LINC00707 can be detected in cytoplasm and it may sponge miR-199-3p to regulate chondrocyte proliferation and apoptosis in OA. Graphical Abstract
Two acquired mouse Y chromosome-linked genes, Prssly and Teyorf1, are dispensable for male fertility

Abstract Prssly (Protease, serine-like, Chr Y) and Teyorf1 (Testis expressed, chromosome Y open reading frame 1) are two acquired single-copy genes located on the distal tip of the non-pairing short arm of the mouse Y chromosome adjacent to telomeric sequence. Both genes lack X chromosome-linked homologues and are expressed in testicular germ cells. We first performed analysis of Prssly and Teyorf1 genomic sequences and demonstrated that previously reported Prssly sequence is erroneous and the true Prssly sequence is longer and encodes a larger protein than previously estimated. We also confirmed that both genes encode pseudogenes that are not expressed in testes. Next, using CRISPR/Cas9 genome targeting, we generated Prssly and Teyorf1 knockout (KO) mice and characterized their phenotype. To create Prssly KO mice, we targeted the conserved exon 5 encoding a trypsin domain typical for serine proteases. The targeting was successful and resulted in a frame shift mutation that introduced a premature stop codon, with the Prssly KO males retaining only residual transcript expression in testes. The Teyorf1 targeting removed the entire open reading frame of the gene, which resulted in no transcript expression in KO males. Both Prssly KO and Teyorf1 KO males were fertile and had normal testis size and normal sperm number, motility, and morphology. Our findings show that Prssly and Teyorf1 transcripts with potential to encode proteins are dispensable for male fertility. Summary Sentence CRISPR/Cas9 generated Prssly and Teyorf1 knockout mice are fertile and display normal spermatogenic features.
Association study of TAP and HLA‐I gene combination with chronic hepatitis C virus infection in a Han population in China

Host immune system genes play key roles in the progression of chronic hepatitis C virus (HCV) infection. Transporters associated with antigen processing (TAP) play an important role in the loading of viral peptides onto MHC class I molecules. This study aimed to investigate the association between TAP gene polymorphisms and chronic HCV in a Chinese Han population. A total of 232 chronic hepatitis C (CHC) patients and 362 healthy individuals were recruited from the Han population in Yunnan province in southwest China, and a TaqMan SNP genotyping assay was used to detect six single nucleotide polymorphisms (SNPs) of TAP1 and three SNPs of TAP2 genes. The association of the TAP gene with CHC was analysed at the allele, genotype, and haplotype levels. There were no significant differences in the allele and genotype frequencies of these SNPs in the TAP gene between CHC patients and controls after Bonferroni correction. A novel TAP1 allele (TAP1*unknown_1: rs41555220‐rs41549617‐rs1057141‐rs1135216‐rs1057149‐rs41551515: G‐G‐A‐G‐G‐G) was only present in the CHC group, and this allele significantly increased susceptibility to CHC (p = .005, odds ratio [OR] = 11.105. 95% confidence interval [CI]: 1.362–90.558). Homozygous TAP1*03:01/TAP1*03:01 was observed only in the CHC group that exhibited an obvious risk for CHC (p = .002, OR = 9.637, 95% CI: 1.153–80.574). And the haplotype TAP1*unknown_1‐TAP2*01:01 was only present in the CHC group and indicated a significant risk for CHC (p = .002, OR = 9.498, 95% CI: 1.140–79.149). We observed significant interactions among HLA‐A, ‐B,C, TAP1, and TAP2 alleles, and combination analysis revealed that the combination of TAP1*01:01‐TAP2*01:01‐HLA‐B*35:01 was only present in the control group (2.2%) and resulted in significantly increased resistance to CHC (p = .002, OR = 0.096, 95% CI: 0.012–0.759). Whereas, the combination of TAP1*01:01‐TAP2*01:01‐HLA‐C*07:02 and TAP1*03:01‐TAP2*01:01‐HLA‐C*01:02 increased the susceptibility to CHC significantly (p = .001, OR = 2.016, 95% CI: 1.309–3.106 and p = .002, OR = 8.070, 95% CI: 1.018–63.997, respectively). Our results indicated that TAP and HLA‐I may exert a combined effect on CHC susceptibility in the Chinese Han population.
Update on primary plasma cell leukemia

Plasma cell leukemia (PCL) is a rare and highly aggressive plasma cell neoplasm developing in 0.5‒4% of patients with multiple myeloma (MM). The diagnostic criteria were recently revised from 20% to ≥5% of circulating plasma cells in peripheral blood smears. PCL is classified as primary or secondary; primary PCL is when it presents in patients with no MM. Primary PCL shows clinical and laboratory features at presentation that differ from MM and exhibits a dismal prognosis even with the use of effective agents against MM. Therefore, intensive chemotherapy should be initiated immediately after diagnosis, and autologous stem cell transplantation is recommended for transplant-eligible patients. Maintenance therapy after transplantation may reduce the rate of early relapses. We reviewed the definitions of PCL, revised diagnostic criteria, clinical features, and appropriate initial treatments for primary PCL.
Massive expression of cysteine-containing proteins causes abnormal elongation of yeast cells by perturbing the proteasome

Abstract The enhanced green fluorescent protein (EGFP) is considered to be a harmless protein because the critical expression level that causes growth defects is higher than that of other proteins. Here, we found that overexpression of EGFP, but not a glycolytic protein Gpm1, triggered the cell elongation phenotype in the budding yeast Saccharomyces cerevisiae. By the morphological analysis of the cell overexpressing fluorescent protein and glycolytic enzyme variants, we revealed that cysteine content was associated with the cell elongation phenotype. The abnormal cell morphology triggered by overexpression of EGFP was also observed in the fission yeast Schizosaccharomyces pombe. Overexpression of cysteine-containing protein was toxic, especially at high-temperature, while the toxicity could be modulated by additional protein characteristics. Investigation of protein aggregate formation, morphological abnormalities in mutants, and transcriptomic changes that occur upon overexpression of EGFP variants suggested that perturbation of the proteasome by the exposed cysteine of the overexpressed protein causes cell elongation. Overexpression of proteins with relatively low folding properties, such as EGFP, was also found to promote the formation of SHOTA (Seventy kDa Heat shock protein-containing, Overexpression-Triggered Aggregates), an intracellular aggregate that incorporates Hsp70/Ssa1, which induces a heat shock response, while it was unrelated to cell elongation. Evolutionary analysis of duplicated genes showed that cysteine toxicity may be an evolutionary bias to exclude cysteine from highly expressed proteins. The overexpression of cysteine-less moxGFP, the least toxic protein revealed in this study, would be a good model system to understand the physiological state of protein burden triggered by ultimate overexpression of harmless proteins.
Protective effect of miR-33-5p on the M1/M2 polarization of microglia and the underlying mechanism

ABSTRACT This study was aimed to investigate the influence of miR-33-5p on the M1/M2 polarization of microglia and the underlying mechanism. Transcriptome sequencing was performed using microglia from miR-33-5p mimic and control groups. In total, 507 differentially expressed genes, including 314 upregulated genes and 193 downregulated genes, were identified. The subnetwork of module A, which was extracted from the protein–protein interaction networks, mainly contained the downregulated genes. Cdk1,Ccnb,and Cdc20, the members of module-A networks with the highest degrees, possess the potential of being biomarkers of ischemic stroke due to their function in the cell cycle. NFY, a transcription factor, was predicted to have the regulatory relation with nine downregulated genes. Overall, our findings will provide a valuable foundation for genetic mechanisms and treatment studies of ischemic stroke. Graphic abstract
Are You for Real? Perceptions of Authenticity Are Systematically Biased and Not Accurate

Can people accurately perceive who is authentic? Laypeople believe they can tell who is authentic, and they report that authenticity is an important attribute in others (Studies 1a and 1b; N = 369). However, when we directly tested the accuracy of perceived authenticity, we found no significant correlation between self- and other-rated authenticity in two cohorts of adult students in randomly assigned teams (Studies 2 and 3; 4,040 self-other observations). In addition, we found that perceived authenticity was biased in two ways: (a) Other-rated authenticity showed a positivity bias compared with self-ratings, and (b) other-rated authenticity was biased by the rater’s own authenticity. In Study 3, we also investigated authenticity meta-perceptions; although people expect their authenticity to be accurately perceived by others, their meta-perceptions did not correlate with other-rated authenticity. That is, beliefs about the visibility of one’s authenticity are similarly not accurate. Overall, we found no evidence that people can accurately identify who is authentic.
Comparison of Serum Ketone Levels and Cardiometabolic Efficacy of Dapagliflozin versus Sitagliptin among Insulin-Treated Chinese Patients with Type 2 Diabetes Mellitus

Background Insulin-treated patients with long duration of type 2 diabetes mellitus (T2DM) are at increased risk of ketoacidosis related to sodium-glucose co-transporter 2 inhibitor (SGLT2i). The extent of circulating ketone elevation in these patients remains unknown. We conducted this study to compare the serum ketone response between dapagliflozin, an SGLT2i, and sitagliptin, a dipeptidyl peptidase-4 inhibitor, among insulin-treated T2DM patients. Methods This was a randomized, open-label, active comparator-controlled study involving 60 insulin-treated T2DM patients. Participants were randomized 1:1 for 24-week of dapagliflozin 10 mg daily or sitagliptin 100 mg daily. Serum β-hydroxybutyrate (BHB) levels were measured at baseline, 12 and 24 weeks after intervention. Comprehensive cardiometabolic assessments were performed with measurements of high-density lipoprotein cholesterol (HDL-C) cholesterol efflux capacity (CEC), vibration-controlled transient elastography and echocardiography. Results Among these 60 insulin-treated participants (mean age 58.8 years, diabetes duration 18.2 years, glycosylated hemoglobin 8.87%), as compared with sitagliptin, serum BHB levels increased significantly after 24 weeks of dapagliflozin (P=0.045), with a median of 27% increase from baseline. Change in serum BHB levels correlated significantly with change in free fatty acid levels. Despite similar glucose lowering, dapagliflozin led to significant improvements in body weight (P=0.006), waist circumference (P=0.028), HDL-C (P=0.041), CEC (P=0.045), controlled attenuation parameter (P=0.007), and liver stiffness (P=0.022). Average E/e’, an echocardiographic index of left ventricular diastolic dysfunction, was also significantly lower at 24 weeks in participants treated with dapagliflozin (P=0.037). Conclusion Among insulin-treated T2DM patients with long diabetes duration, compared to sitagliptin, dapagliflozin modestly increased ketone levels and was associated with cardiometabolic benefits.
Valve‐in‐valve transcatheter aortic valve replacement or re‐surgical aortic valve replacement in degenerated bioprostheses: A systematic review and meta‐analysis of short and midterm results

Despite limited to short and midterm outcomes, valve‐in‐valve (ViV) transcatheter aortic valve implantation (TAVI) has emerged as a valid alternative to re‐surgical aortic valve replacement (re‐SAVR) for high‐ and intermediate‐risk patients with degenerated surgical bioprosthesis.