Miracle-dz/UniFAR-FTUs huggingface dataset:
The experience of occupational identity disruption post stroke: a systematic review and meta-ethnography

Abstract Purpose After a stroke, most individuals cannot participate in some of their valued occupations, disrupting their occupational identity. The aim of this interpretive synthesis is to systematically review the existing body of research to develop an understanding of the experience of occupational identity disruption post-stroke. Methods A systematic review of the literature that included nine electronic databases, reference lists screening and grey literature checking was completed. Ten studies published between January 2012 and October 2018 were included. Meta-ethnography was used for the interpretive synthesis. Findings A three-staged process model was created. Stage 1 illustrates the four factors involved in occupational identity disruption: “A fragmented and externalized body”, “losing control, freedom and independence”, “changed social and familial interactions”, and “occupational participation loss”. Stage two explains the experience of occupational identity disruption: “Occupational identity loss”, “a de-valued self”, and “threat to identity continuity”. Stage three shows the individual’s coping strategies: “protecting one’s self”, “social isolation”, and “re-inventing one’s occupational self”. Conclusions Stroke impacts on an individual’s occupational identity, compromising the continuity of a stroke survivor’s sense of self. However, occupational identity is fluid; this can be used to support individuals to re-invent the occupational self. A conceptual model was developed to support the application of these results in clinical practice. Implications for rehabilitation Occupational identity disruption impacts on stroke survivors’ self-value and sense of self continuity. Health care professionals’ understanding of stroke survivors experience of occupational identity disruption could contribute to realign stroke survivors and clinicians’ rehabilitation goals. Occupational participation and social interaction should be core aspects of stroke rehabilitation. Occupation-focus rehabilitation could provide opportunities for stroke survivors to pursue their desired occupational identity.
A review on the latest advances in extraction and analysis of artemisinin.

INTRODUCTION Artemisinin (1), a well-known natural antimalarial drug, is a sesquiterpene lactone that contains a unique peroxide bridge. Since its discovery, the amount of research into the analysis of artemisinin has increased considerably, and it has been further intensified since the Noble Prize win by Tu Youyou in the year 2015 for the discovery of artemisinin. OBJECTIVE To review literature on the extraction and analysis of artemisinin, published during 2017-present, and to present an appraisal of those methods. METHODOLOGY Extensive literature search was carried out which involved, but not limited to, the use of, various databases, like Web of Knowledge, PubMed and Google Scholar, and relevant published materials including published books. The keywords used, in various combinations, with artemisinin being present in all combinations, in the search were artemisinin, Artemisia annua, analysis, extraction, quantitative, qualitative and quality control. RESULTS During the period covered in this review, several methods of analysis of artemisinin have been reported, the most of which were liquid chromatography (LC)-based methods. However, the use of new methods like near-infrared analysis, fluorometirc analysis and molecular imprinting, and a significant increase in the use of computational tools have been observed. Mainly several methods involving supercritical fluid extraction and ultrasound-assisted extraction of artemisinin have dominated the extraction area. CONCLUSIONS Newer analytical tools, as well as improved protocols for the known analytical tools, for qualitative and quantitative determination of artemisinin (1), have been made available by various researchers during the period covered by this review. Supercritical fluid extraction and ultrasound-assisted extraction are still the methods of choice for extraction of artemisinin.
Prediction of models for ordered solvent in macromolecular structures by a classifier based upon resolution-independent projections of local feature data

PeakProbe facilitates the automated modelling of ordered solvent in macromolecular crystal structures by analysing features of the electron density and chemical environment surrounding a given coordinate. The extracted data are transformed to a resolution-independent score space and likely solvent models are predicted based on the frequency distributions observed in a large-scale sample of the PDB.
Anterior Cruciate Ligament Repair in a Professional Soccer Player Using Internal Brace Ligament Augmentation: A Case Report Focusing on Rehabilitation.

BACKGROUND Anterior cruciate ligament (ACL) reconstruction with hamstring or patellar tendon autograft has been the gold standard for the operative treatment of an ACL rupture for many years. Repair with Internal Brace Ligament Augmentation (IBLA) is a new technique that uses ultra-high strength tape (FiberTape, Arthrex, Naples, FL, USA) to bridge the ligament. This technique reinforces the ligament as a secondary stabiliser, encouraging natural healing of the ligament by protecting it during the healing phase and supporting early mobilisation. CASE DESCRIPTION This retrospective case report focuses on the rehabilitation of a 21-year-old male professional soccer player who ruptured his ACL in a contact injury whilst playing a competitive game. He underwent ACL repair with IBLA two weeks following injury. The six-month rehabilitation programme consisted of gradual progressions for mobility, proprioception, strengthening, cardiovascular maintenance and running in conjunction with physiotherapy to assist with the maintenance of soft tissue quality, pain management and control of oedema. RESULTS After completing the rehabilitation programme, the patient returned to unrestricted sporting activity within six months. At 18-month follow-up, the patient continues to play at the same competitive level without any issues. CONCLUSION This rehabilitation programme after ACL repair with IBLA successfully enabled a professional soccer player to return to his pre-injury playing level.
Impact of repetitive transcranial magnetic stimulation on generalized anxiety disorder in treatment-resistant depression.

BACKGROUND Published evidence indicates the value of repetitive transcranial magnetic stimulation (rTMS) for generalized anxiety disorder in patients with treatment-resistant depression (TRD). As a part of routine clinical service, patients with TRD received right dorsolateral prefrontal cortex (DLPFC) inhibitory rTMS immediately prior to left DLPFC depression treatment delivered according to a US Food and Drug Administration protocol. METHODS A retrospective investigation of routinely collected data between 2016 and 2018 was undertaken. Measures used were the clinicianrated Clinical Global Impressions-Severity (CGI-S) scale and Hamilton Depression Rating scale (HAM-D), and the patient-rated Generalized Anxiety Disorder scale-7 (GAD-7) and Patient Health Questionnaire (PHQ-9). The outcome data of 61 patients with TRD were analyzed. The sample included patients with comorbid psychiatric diagnoses. RESULTS Response and remission rates, respectively, were 17.1% and 27.3% on the GAD-7; 20.7% and 12.5% on the HAM-D; 19% and 24% on the PHQ-9; and 20% and 23.5% on the CGI-S. Post-treatment scores significantly improved on the GAD-7, HAM-D, and CGI-S scales, with medium to large effect sizes (.61, .62, and .86 respectively). CONCLUSIONS Results indicate the potential value of rTMS in treating anxiety in patients who are referred for rTMS for TRD. Well-designed and adequately powered randomized controlled trials are required to determine clinical recommendations.
Remodeling Membrane Binding by Mono-Ubiquitylation

Ubiquitin (Ub) receptors respond to ubiquitylation signals. They bind ubiquitylated substrates and exert their activity in situ. Intriguingly, Ub receptors themselves undergo rapid ubiquitylation and deubiquitylation. Here we asked what is the function of ubiquitylation of Ub receptors? We focused on yeast epsin, a Ub receptor that decodes the ubiquitylation signal of plasma membrane proteins into an endocytosis response. Using mass spectrometry, we identified lysine-3 as the major ubiquitylation site in the epsin plasma membrane binding domain. By projecting this ubiquitylation site onto our crystal structure, we hypothesized that this modification would compete with phosphatidylinositol-4,5-bisphosphate (PIP2) binding and dissociate epsin from the membrane. Using an E. coli-based expression of an authentic ubiquitylation apparatus, we purified ubiquitylated epsin. We demonstrated in vitro that in contrast to apo epsin, the ubiquitylated epsin does not bind to either immobilized PIPs or PIP2-enriched liposomes. To test this hypothesis in vivo, we mimicked ubiquitylation by the fusion of Ub at the ubiquitylation site. Live cell imaging demonstrated that the mimicked ubiquitylated epsin dissociates from the membrane. Our findings suggest that ubiquitylation of the Ub receptors dissociates them from their products to allow binding to a new ubiquitylated substrates, consequently promoting cyclic activity of the Ub receptors.
Endothelium-Dependent Hyperpolarization (EDH) in Diabetes: Mechanistic Insights and Therapeutic Implications

Diabetes mellitus is one of the major risk factors for cardiovascular disease and is an important health issue worldwide. Long-term diabetes causes endothelial dysfunction, which in turn leads to diabetic vascular complications. Endothelium-derived nitric oxide is a major vasodilator in large-size vessels, and the hyperpolarization of vascular smooth muscle cells mediated by the endothelium plays a central role in agonist-mediated and flow-mediated vasodilation in resistance-size vessels. Although the mechanisms underlying diabetic vascular complications are multifactorial and complex, impairment of endothelium-dependent hyperpolarization (EDH) of vascular smooth muscle cells would contribute at least partly to the initiation and progression of microvascular complications of diabetes. In this review, we present the current knowledge about the pathophysiology and underlying mechanisms of impaired EDH in diabetes in animals and humans. We also discuss potential therapeutic approaches aimed at the prevention and restoration of EDH in diabetes.
A Milled Microdevice to Advance Glia-Mediated Therapies in the Adult Nervous System

Neurodegenerative disorders affect millions of adults worldwide. Neuroglia have become recent therapeutic targets due to their reparative abilities in the recycling of exogenous neurotoxins and production of endogenous growth factors for proper functioning of the adult nervous system (NS). Since neuroglia respond effectively to stimuli within in vivo environments on the micron scale, adult glial physiology has remarkable synergy with microscale systems. While clinical studies have begun to explore the reparative action of Müller glia (MG) of the visual system and Schwann Cells (ShC) of the peripheral NS after neural injury, few platforms enable the study of intrinsic neuroglia responses to changes in the local microenvironment. This project developed a low-cost, benchtop-friendly microfluidic system called the glia line system, or gLL, to advance the cellular study needed for emerging glial-based therapies. The gLL was fabricated using elastomeric kits coupled with a metal mold milled via conventional computer numerical controlled (CNC) machines. Experiments used the gLL to measure the viability, adhesion, proliferation, and migration of MG and ShC within scales similar to their respective in vivo microenvironments. Results illustrate differences in neuroglia adhesion patterns and chemotactic behavior significant to advances in regenerative medicine using implants and biomaterials, as well as cell transplantation techniques. Data showed highest survival and proliferation of MG and ShC upon laminin and illustrated a four-fold and two-fold increase of MG migration to dosage-dependent signaling from vascular endothelial growth factor (VEGF) and epidermal growth factor (EGF), respectively, as well as a 20-fold increase of ShC migration toward exogenous brain-derived neurotrophic factor (BDNF), compared to media control. The ability to quantify these biological parameters within the gLL offers an effective and reliable alternative to photolithography study neuroglia in a local environment ranging from the tens to hundreds of microns, using a low-cost and easily fabricated system.
Subjective well-being in China’s changing society

Significance We reevaluate the level of subjective well-being in China with data from the China Household Finance Study. The analysis shows that in contrast to the decline in well-being reported in earlier studies, all groups report increased quality of life in the second decade of the 21st century. The analysis shows that the gap across rural and urban respondents is narrowing. Probit models of the levels of well-being show that social capital, measured as the perceived feelings of safety and security, community participation, and whether society is perceived to be fair, influence reported well-being. Household income, assets, health, being married, and the quality of neighborhood infrastructure, measured by availability of parks, primary schools, and hospitals, contributes positively to subjective well-being. There is now recognition that a population’s overall level of well-being is defined not just by income and wealth. Where we live and who we interact with are likely to be equally important in our overall levels of satisfaction with our lives. This thinking has stimulated studies of subjective well-being, or happiness, at both national and local scales. These studies suggest that where you live does matter, although it is health and family status that have the most direct effects on well-being. In this study, we use a detailed dataset on well-being from the China Household Finance Survey to reexamine well-being across China, where profound socioeconomic changes are taking place. The study controls for self-reported health and examines subjective well-being across extensive and varied Chinese urban and rural environments. We find that the earlier pessimism about China’s well-being, which emphasized declining happiness, may be misplaced. We make two contributions: first, we show a rising level of subjective well-being, and second, we show that there is a narrowing gap in well-being across different social indicators. Methodologically, we bring in the perspectives of both social capital and geographic context.
Cerebellar hypermetabolism in alcohol use disorder: compensatory mechanism or maladaptive plasticity?

BACKGROUND Despite severe structural brain abnormalities within the fronto-cerebellar circuit (FCC), cerebellar metabolism studied with FDG-PET is relatively preserved in patients with alcohol use disorder (AUD). The compensatory role of the cerebellum has been explored mainly through fMRI examination of AUD patients with preserved level of performance. The present study aims at examining cerebellar metabolism and its relationship with regional brain metabolism and neuropsychological functioning in AUD patients. METHODS Thirty-two recently detoxified AUD patients and 23 controls underwent an FDG-PET examination at rest. Participants also performed a neuropsychological battery assessing executive functions, verbal memory and ataxia. RESULTS Compared to controls, AUD patients had higher glucose-uptake in the cerebellar lobules VIII, in association with hypometabolism notably in several nodes of the FCC. Cerebellar hypermetabolism correlated negatively with regional hypometabolism in the premotor and frontal cortices. This pattern of regional hypermetabolism and hypometabolism related to ataxia and working memory deficits. CONCLUSIONS These specific brain-behaviour relationships do not fulfil the criteria for brain compensatory processes. Cerebellar hypermetabolism may rather reflect the involvement of different pathological mechanisms, leading to a maladaptive plasticity phenomenon within the FCC in AUD patients who are early in abstinence. Further studies are required to examine the contributions of structural and functional connectivity alterations in the cerebellar hypermetabolism and the changes of these pathological mechanisms with abstinence or relapse. This article is protected by copyright. All rights reserved.
Model-based Spectral Library Approach for Bacterial Identification via Membrane Glycolipids.

By circumventing the need for a pure colony, MALDI-TOF Mass Spectrometry of bacterial membrane glycolipids (lipid A) has the potential to identify microbes more rapidly than protein-based methods. However, currently available bioinformatics algorithms (e.g. dot products) do not work well with glycolipid mass spectra like those produced by lipid A, the membrane anchor of lipopolysaccharide. To address this issue, we propose a spectral library approach coupled with a machine learning technique to more accurately identify microbes. Here, we demonstrate the performance of the model-based spectral library approach for microbial identification using approximately a thousand mass spectra collected from multidrug-resistant bacteria. At false discovery rates < 1%, our approach identified many more bacterial species than the existing approaches such as the Bruker Biotyper and characterized over 97% of their phenotypes accurately. As the diversity in our glycolipid mass spectral library increases, we anticipate that it will provide valuable information to more rapidly treat infected patients.
Revisiting the Dependence of Poisson’s Ratio on Liquid Fragility and Atomic Packing Density in Oxide Glasses

Poisson’s ratio (ν) defines a material’s propensity to laterally expand upon compression, or laterally shrink upon tension for non-auxetic materials. This fundamental metric has traditionally, in some fields, been assumed to be a material-independent constant, but it is clear that it varies with composition across glasses, ceramics, metals, and polymers. The intrinsically elastic metric has also been suggested to control a range of properties, even beyond the linear-elastic regime. Notably, metallic glasses show a striking brittle-to-ductile (BTD) transition for ν-values above ~0.32. The BTD transition has also been suggested to be valid for oxide glasses, but, unfortunately, direct prediction of Poisson’s ratio from chemical composition remains challenging. With the long-term goal to discover such high-ν oxide glasses, we here revisit whether previously proposed relationships between Poisson’s ratio and liquid fragility (m) and atomic packing density (Cg) hold for oxide glasses, since this would enable m and Cg to be used as surrogates for ν. To do so, we have performed an extensive literature review and synthesized new oxide glasses within the zinc borate and aluminoborate families that are found to exhibit high Poisson’s ratio values up to ~0.34. We are not able to unequivocally confirm the universality of the Novikov-Sokolov correlation between ν and m and that between ν and Cg for oxide glass-formers, nor for the organic, ionic, chalcogenide, halogenide, or metallic glasses. Despite significant scatter, we do, however, observe an overall increase in ν with increasing m and Cg, but it is clear that additional structural details besides m or Cg are needed to predict and understand the composition dependence of Poisson’s ratio. Finally, we also infer from literature data that, in addition to high ν, high Young’s modulus is also needed to obtain glasses with high fracture toughness.
Draft Genome Sequence of Desulfosporosinus fructosivorans Strain 63.6FT, Isolated from Marine Sediment in the Baltic Sea

Desulfosporosinus fructosivorans strain 63.6FT is a strictly anaerobic, spore-forming, sulfate-reducing bacterium isolated from marine sediment in the Baltic Sea. Here, we report the draft genome sequence of D. fructosivorans 63.6FT. ABSTRACT Desulfosporosinus fructosivorans strain 63.6FT is a strictly anaerobic, spore-forming, sulfate-reducing bacterium isolated from marine sediment in the Baltic Sea. Here, we report the draft genome sequence of D. fructosivorans 63.6FT.
Pediatric Pulmonology

Correspondence Henry J. Rozycki, MD, Children’s Hospital of Richmond at VCU, Box 980646, Richmond, VA 23298‐0646. Email: henry.rozycki@vcuhealth.org ABSTRACT Neurally adjusted ventilatory assistance (NAVA) can overcome technical difficulties with synchronizing noninvasive ventilation breaths with the patient, a modality often used in very low birthweight infants (VLBW) with apnea of prematurity (AOP). This study is a retrospective single‐center investigation into whether NAVA‐synchronized noninvasive (niNAVA) ventilation is better than nonsynchronized (nasal intermittent positive pressure ventilation [nIPPV]) for symptomatic apnea in VLBW infants. Nursing records of apnea, bradycardia, and/or desaturations were abstracted from the electronic medical records of 108 VLBW infants admitted to the neonatal intensive care unit (NICU) from 2015 to 2017 who received either of the two modalities, 61 epochs of niNAVA totaling 488 days and 103 epochs of nIPPV totaling 886.5 days. niNAVA was associated with a significant reduction in the number of isolated bradycardic events/day (0.48 ± 0.14 vs 1.35 ±0.27; P= .019) and overall bradycardias/day (2.42 ±0.47 vs 4.02±0.53; P= .042) and there were more epochs with no events with niNAVA compared with nIPPV (23.0% vs 6.8%; P= .004). These results justify a prospective trial of NAVA‐synchronized noninvasive ventilation for VLBW infants with caffeine‐resistant AOP.
A New Black Elderberry Dye Enriched in Antioxidants Designed for Healthy Sweets Production

The aim of the study was to obtain a dye from black elder fruits and flowers and to study their potential in production of jellies with high antioxidant activity. Three dyes were produced by lyophilization of aqueous extracts: (1) fruits dye (F), (2) flowers dye (FL), and (3) fruits and flowers dye (F + FL). Their polyphenol profiles were compared by means of ultra-performance liquid chromatography (UPLC). The antioxidant activity [ferric reducing/antioxidant power assay (FRAP) and DPPH radicals scavenging test and total phenolics were compared by spectrophotometric methods. Jellies were produced from agar and gelatin with the addition of three obtained dyes, and their antioxidant water- and lipid soluble fractions were tested with a Photochem device. Results indicated that black elder fruits are rich in anthocyanins, especially cyanidin-3-O-sambubioside (7.56 mg/g d.w.), while flowers are rich in polyphenols, especially chlorogenic acid (2.82 mg/g d.w.) and rutin (4.04 mg/g d.w.). FL dye exhibited higher antioxidant activity compared to F dye (for about 30–40%), regardless of the used method, whereas F + FL dye was characterized by intermediate antioxidant activity. Jellies produced with the addition of FL dye had better antioxidant properties but unattractive color and unpleasant taste, but the use of F + FL dye created a product of favorable organoleptic properties and antioxidant activity comparable to jellies with F dye addition.