Miracle-dz/UniFAR-FTUs huggingface dataset:
Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops

Protecting food crops from viral pathogens is a significant challenge for agriculture. An integral approach to genome-editing, known as CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR associated protein 9), is used to produce virus-resistant cultivars. The CRISPR/Cas9 tool is an essential part of modern plant breeding due to its attractive features. Advances in plant breeding programs due to the incorporation of Cas9 have enabled the development of cultivars with heritable resistance to plant viruses. The resistance to viral DNA and RNA is generally provided using the Cas9 endonuclease and sgRNAs (single-guide RNAs) complex, targeting particular virus and host plant genomes by interrupting the viral cleavage or altering the plant host genome, thus reducing the replication ability of the virus. In this review, the CRISPR/Cas9 system and its application to staple food crops resistance against several destructive plant viruses are briefly described. We outline the key findings of recent Cas9 applications, including enhanced virus resistance, genetic mechanisms, research strategies, and challenges in economically important and globally cultivated food crop species. The research outcome of this emerging molecular technology can extend the development of agriculture and food security. We also describe the information gaps and address the unanswered concerns relating to plant viral resistance mediated by CRISPR/Cas9.
Pharmacokinetics, Biodistribution, and Biosafety of PEGylated Gold Nanoparticles In Vivo

Despite the obvious advantages of gold nanoparticles for biomedical applications, controversial and incomplete toxicological data hamper their widespread use. Here, we present the results from an in vivo toxicity study using gold nanoparticles coated with polyethylene glycol (PEG-AuNPs). The pharmacokinetics and biodistribution of PEG-AuNPs were examined in the rat’s liver, lung, spleen, and kidney after a single i.v. injection (0.7 mg/kg) at different time intervals. PEG-AuNPs had a relatively long blood circulation time and accumulated primarily in the liver and spleen, where they remained for up to 28 days after administration. Increased cytoplasmic vacuolation in hepatocytes 24 h and 7 days after PEG-AuNPs exposure and apoptotic-like cells in white splenic pulp 24 h after administration has been detected, however, 28 days post-exposure were no longer observed. In contrast, at this time point, we identified significant changes in lipid metabolism, altered levels of liver injury markers, and elevated monocyte count, but without marked biological relevance. In blood cells, no DNA damage was present in any of the studied time intervals, with the exception of DNA breakage transiently detected in primary kidney cells 4 h post-injection. Our results indicate that the tissue accumulation of PEG-AuNPs might result in late toxic effects.
Draft Genome Assemblies of Two Cryptosporidium hominis Isolates from New Zealand

Cryptosporidium hominis is a protozoan parasite that causes gastrointestinal disease in humans worldwide. Here, we report on draft whole-genome sequences of two clinical isolates of C. hominis that were purified from patients with cryptosporidiosis in New Zealand. ABSTRACT Cryptosporidium hominis is a protozoan parasite that causes gastrointestinal disease in humans worldwide. Here, we report on draft whole-genome sequences of two clinical isolates of C. hominis that were purified from patients with cryptosporidiosis in New Zealand.
Improvement of Soil Microbial Diversity through Sustainable Agricultural Practices and Its Evaluation by -Omics Approaches: A Perspective for the Environment, Food Quality and Human Safety

Soil is one of the key elements for supporting life on Earth. It delivers multiple ecosystem services, which are provided by soil processes and functions performed by soil biodiversity. In particular, soil microbiome is one of the fundamental components in the sustainment of plant biomass production and plant health. Both targeted and untargeted management of soil microbial communities appear to be promising in the sustainable improvement of food crop yield, its nutritional quality and safety. –Omics approaches, which allow the assessment of microbial phylogenetic diversity and functional information, have increasingly been used in recent years to study changes in soil microbial diversity caused by agronomic practices and environmental factors. The application of these high-throughput technologies to the study of soil microbial diversity, plant health and the quality of derived raw materials will help strengthen the link between soil well-being, food quality, food safety and human health.
Transcranial Direct Current Stimulation Decreases the Decline of Speed during Repeated Sprinting in Basketball Athletes

The purpose of this study was to determine whether transcranial direct current stimulation (tDCS) can improve countermovement jump performance, fatigue index and alleviate the speed decline during repeated shuttle sprints in trained basketball players. Thirteen trained basketball players were divided into the tDCS trial and sham trial by the random crossover design. The tDCS trial was stimulated with 2-mA current in the M1 area in the middle of the top of the head for 20 min. For the sham trial, the current was turned off after 5 s, stopping the electrical stimulation. After warming up, the players underwent countermovement jump test, weighted countermovement jump test and then performed 40 × 15-m sprints with with a 1:4 exercise: rest ratio. The jump height, sprinting time, fatigue index, heart rate and rating of perceived exertion (RPE) were analyzed by paired-sample t-test, when significance was discovered by two-way repeated measures analysis of variance. The study results revealed that the tDCS trial significantly increase the countermovement jump performance (p = 0.04), decrease the sprinting time (p = 0.016), and had improved fatigue index during the sprinting process (p = 0.009). However, the heart rate and RPE during sprinting were nonsignificantly different between the trials. This study has identified that tDCS can decrease the speed decline, fatigue index during sprinting and increase countermovement jump performance without affecting heart rate or the rating of perceived exertion.
Iron Released after Cryo-Thermal Therapy Induced M1 Macrophage Polarization, Promoting the Differentiation of CD4+ T Cells into CTLs

Macrophages play critical roles in both innate and adaptive immunity and are known for their high plasticity in response to various external signals. Macrophages are involved in regulating systematic iron homeostasis and they sequester iron by phagocytotic activity, which triggers M1 macrophage polarization and typically exerts antitumor effects. We previously developed a novel cryo-thermal therapy that can induce the mass release of tumor antigens and damage-associated molecular patterns (DAMPs), promoting M1 macrophage polarization. However, that study did not examine whether iron released after cryo-thermal therapy induced M1 macrophage polarization; this question still needed to be addressed. We hypothesized that cryo-thermal therapy would cause the release of a large quantity of iron to augment M1 macrophage polarization due to the disruption of tumor cells and blood vessels, which would further enhance antitumor immunity. In this study, we investigated iron released in primary tumors, the level of iron in splenic macrophages after cryo-thermal therapy and the effect of iron on macrophage polarization and CD4+ T cell differentiation in metastatic 4T1 murine mammary carcinoma. We found that a large amount of iron was released after cryo-thermal therapy and could be taken up by splenic macrophages, which further promoted M1 macrophage polarization by inhibiting ERK phosphorylation. Moreover, iron promoted DC maturation, which was possibly mediated by iron-induced M1 macrophages. In addition, iron-induced M1 macrophages and mature DCs promoted the differentiation of CD4+ T cells into the CD4 cytolytic T lymphocytes (CTL) subset and inhibited differentiation into Th2 and Th17 cells. This study explains the role of iron in cryo-thermal therapy-induced antitumor immunity from a new perspective.
Relationships between Renewable Energy and the Prevalence of Morbidity in the Countries of the European Union: A Panel Regression Approach

The main objective of the presented study was to examine the associations between the use of renewable energy sources in selected sectors (transport, electricity, heating, and cooling) and the prevalence of selected groups of diseases in the European Union, with an emphasis on the application of statistical methods considering the structure of data. The analyses included data on 27 countries of the European Union from 2010 to 2019 published in the Eurostat database and the Global Burden of Disease Study. Panel regression models (pooling model, fixed (within) effects model, random effects model) were primarily used in analytical procedures, in which a panel variable was represented by countries. In most cases, positive and significant associations between the use of renewable energy sources and the prevalence of diseases were confirmed. The results of panel regression models could be generally interpreted as meaning that renewable energy sources are associated with the prevalence of diseases such as cardiovascular diseases, diabetes and kidney diseases, digestive diseases, musculoskeletal disorders, neoplasms, sense organ diseases, and skin and subcutaneous diseases at a significance level (α) of 0.05 and lower. These findings could be explained by the awareness of the health problem and the response in the form of preference for renewable energy sources. Regarding statistical methods used for country data or for data with a specific structure, it is recommended to use the methods that take this structure into account. The absence of these methods could lead to misleading conclusions.
Ultraflexible and Mechanically Strong Polymer/Polyaniline Conductive Interpenetrating Nanocomposite via In Situ Polymerization of Vinyl Monomer

Simultaneous enhancement of conductivity and mechanical properties for polyaniline/polymer nanocomposite still remains a big challenge. Here, a reverse approach via in situ polymerization (RIP) of vinyl monomers in waterborne polyaniline dispersion was raised to prepare conductive polyaniline (GPANI)/polyacrylate (PMB) interpenetrating polymer (GPANI-PMB) nanocomposite. GPANI/PMB physical blend was simultaneously prepared as reference. The conductive GPANI-PMB nanocomposite film with compact pomegranate-shape morphology is homogeneous, ultraflexible and mechanically strong. With incorporating a considerable amount of PMB into GPANI via the RIP method, only a slight decrease from 3.21 to 2.80 S/cm was detected for the conductivity of GPANI-PMB, while the tensile strength significantly increased from 25 to 43.5 MPa, and the elongation at break increased from 40% to 234%. The water absorption of GPANI-PMB3 after 72 h immersion decreased from 24.68% to 10.35% in comparison with GPANI, which is also higher than that of GPANI/PMB. The conductivity and tensile strength of GPANI-PMB were also much higher than that of GPANI/PMB (0.006 S/cm vs. 5.59 MPa). Moreover, the conductivity of GPANI-PMB remained almost invariable after folding 200 times, while that of GPANI/PMB decreased by almost half. This RIP approach should be applicable for preparing conventional conductive polymer nanocomposite with high conductivity, high strength and high flexibility.
Cluster of Angiostrongyliasis Cases Following Consumption of Raw Monitor Lizard in the Lao People’s Democratic Republic and Review of the Literature

Angiostrongyliasis in humans causes a range of symptoms from mild headache and myalgia to neurological complications, coma and death. Infection is caused by the consumption of raw or undercooked intermediate or paratenic hosts infected with Angiostrongylus cantonensis or via contaminated vegetables or water. We describe a cluster of cases involved in the shared meal of wild raw monitor lizard in the Lao PDR. Seven males, aged 22–36 years, reported headaches, abdominal pain, arthralgia, myalgia, nausea/vomiting, diarrhea, neurological effects and loss of appetite. Five were admitted to hospital. The final diagnosis was made by clinical presentation and case history, and positive A. cantonensis PCR for two cases. All hospitalized patients recovered fully following supportive treatment. The remaining two individuals sought local home remedies and made full recovery. Whilst most published reports concern infections via consumption of molluscs, few detailed reports exist on infections that result from the consumption of reptiles and there exists little awareness in Lao PDR. This case cluster, which originates from a single meal, highlights the potential public health risk of the consumption of raw and wild-caught meat in Lao PDR and the Southeast Asia region. Without specific diagnostics, clinical history and the consideration of recent food consumption are important when evaluating patients.
Interhelical H-Bonds Modulate the Activity of a Polytopic Transmembrane Kinase

DesK is a Histidine Kinase that allows Bacillus subtilis to maintain lipid homeostasis in response to changes in the environment. It is located in the membrane, and has five transmembrane helices and a cytoplasmic catalytic domain. The transmembrane region triggers the phosphorylation of the catalytic domain as soon as the membrane lipids rigidify. In this research, we study how transmembrane inter-helical interactions contribute to signal transmission; we designed a co-expression system that allows studying in vivo interactions between transmembrane helices. By Alanine-replacements, we identified a group of polar uncharged residues, whose side chains contain hydrogen-bond donors or acceptors, which are required for the interaction with other DesK transmembrane helices; a particular array of H-bond- residues plays a key role in signaling, transmitting information detected at the membrane level into the cell to finally trigger an adaptive response.
Biomimetic Approach for the Elaboration of Highly Hydrophobic Surfaces: Study of the Links between Morphology and Wettability

This investigation of morphology-wetting links was performed using a biomimetic approach. Three natural leaves’ surfaces were studied: two bamboo varieties and Ginkgo Biloba. Multiscale surface topographies were analyzed by SEM observations, FFT, and Gaussian filtering. A PDMS replicating protocol of natural surfaces was proposed in order to study the purely morphological contribution to wetting. High static contact angles, close to 135∘, were measured on PDMS replicated surfaces. Compared to flat PDMS, the increase in static contact angle due to purely morphological contribution was around 20∘. Such an increase in contact angle was obtained despite loss of the nanometric scale during the replication process. Moreover, a significant decrease of the hysteresis contact angle was measured on PDMS replicas. The value of the contact angle hysteresis moved from 40∘ for flat PDMS to less than 10∘ for textured replicated surfaces. The wetting behavior of multiscale textured surfaces was then studied in the frame of the Wenzel and Cassie–Baxter models. Whereas the classical laws made it possible to describe the wetting behavior of the ginkgo biloba replications, a hierarchical model was developed to depict the wetting behavior of both bamboo species.
Determination of five saikosaponins in Bupleurum yinchowense by HPLC

A method was established for determining five saikosaponins simultaneously,including saikosaponins a,c,d,e and f in the root of Bupleurum yinchowense by HPLC for the first time.Separation was achieved by A Hibar RT C18 column(250 mm×4.6 mm,5 μm) using acetonitrile and water as the mobile phase with gradient elution at the f1ow rate of 1.0 mL/min,column temperature at 30 ℃ and the wavelength of UV detection was 210 nm.There were good linear correlations between the studied concentrations of the five saikosaponins and their chromatographic peak areas,and the correlation coefficients were 0.9992-0.9998. The recoveries of saikosaponins a and d were 101.5 and 98.5%,respectively.The RSD of precision and accuracy was less than 3%. This method is simple,accurate and reproducible,and also provides a reference for establishing the multiple-value quality control method for Radix Buplerui and the use of B.yinchowense as a medicine.
Sustainable, Highly Efficient and Superhydrophobic Fluorinated Silica Functionalized Chitosan Aerogel for Gravity-Driven Oil/Water Separation

A superhydrophobic fluorinated silica functionalized chitosan (F-CS) aerogel is constructed and fabricated by a simple and sustainable method in this study in order to achieve highly efficient gravity-driven oil/water separation performance. The fluorinated silica functionalization invests the pristine hydrophilic chitosan (CS) aerogel with promising superhydrophobicity with a water contact angle of 151.9°. This novel F-CS aerogel possesses three-dimensional structure with high porosity as well as good chemical stability and mechanical compression property. Moreover, it also shows striking self-cleaning performance and great oil adsorption capacity. Most importantly, the as-prepared aerogels exhibits fast and efficient separation of oil/water mixture by the gravity driven process with high separation efficiency. These great performances render the prepared F-CS aerogel a good candidate for oil/water separation in practical industrial application.
Current Systemic Treatment Options in Metastatic Urothelial Carcinoma after Progression on Checkpoint Inhibition Therapy—A Systemic Review Combined with Single-Group Meta-Analysis of Three Studies Testing Enfortumab Vedotin

Simple Summary Currently, little is known about what therapeutic options exist for the treatment of metastatic urothelial carcinoma (mUC) after a therapy with checkpoint inhibitors (CPI). In the context of this systemic review, five therapy regimens tested in the post-CPI setting with adequate data were identified: Chemotherapy (CT), Ramucirumab plus Docetaxel, Erdafitinib (Erd), Enfortumab vedotin (EV), and Sacituzumab govitecan (SG). Most data were available on EV, and the results of three studies testing the agent were combined via single-group meta-analysis. For EV, the objective response rate was 42.1% compared to 17.9% for CT in a similar setting. EV was also ahead in progression-free survival (5.9 months with EV vs. 3.7 months with CT) and overall survival (12.8 months with EV vs. 9.0 months with CT). Further research is needed on the question of which patients’ subcollectives particularly benefit from which therapeutic approach. Abstract Background: In the first and second-line therapy of metastatic urothelial carcinoma (mUC), checkpoint inhibitors (CPI) such as Pembrolizumab and Atezolizumab have been widely implemented. Little is currently known about what therapeutic options are effective after therapy with CPI. This article presents a systemic review of current treatment options in this setting. Methods: From August 2020 to 15 April 2021, a literature search was performed through the PubMed/Medline. Subsequently, a single-group meta-analysis of three studies testing Enfortumab vedotin (EV) was conducted. Results: Five therapy regimens tested in the post-CPI setting with adequate data were identified: Chemotherapy (CT), Ramucirumab plus Docetaxel, Erdafitinib (Erd), EV, and Sacituzumab govitecan (SG). In n = 74 + 125 + 288 patients, the single-group meta-analysis showed an objective response rate of 42.1% for EV compared to 17.9% for CT in a similar setting. EV was also ahead in progression free survival (5.9 months with EV vs. 3.7 months with CT) and overall survival (12.8 months with EV vs. 9.0 months with CT). Conclusion: Most data are currently available for EV. Further research is needed on the question of which patients’ subcollectives particularly benefit from which therapeutic approach.
Energy-Efficient Packet Forwarding Scheme Based on Fuzzy Decision-Making in Underwater Sensor Networks

Underwater Wireless Sensor Networks (UWSNs) are subjected to a multitude of real-life challenges. Maintaining adequate power consumption is one of the critical ones, for obvious reasons. This includes proper energy consumption due to nodes close to and far from the sink node (gateway), which affect the overall energy efficiency of the system. These wireless sensors gather and route the data to the onshore base station through the gateway at the sea surface. However, finding an optimum and efficient path from the source node to the gateway is a challenging task. The common reasons for the loss of energy in existing routing protocols for underwater are (1) a node shut down due to battery drainage, (2) packet loss or packet collision which causes re-transmission and hence affects the performance of the system, and (3) inappropriate selection of sensor node for forwarding data. To address these issues, an energy efficient packet forwarding scheme using fuzzy logic is proposed in this work. The proposed protocol uses three metrics: number of hops to reach the gateway node, number of neighbors (in the transmission range of a node) and the distance (or its equivalent received signal strength indicator, RSSI) in a 3D UWSN architecture. In addition, the performance of the system is also tested with adaptive and non-adaptive transmission ranges and scalable number of nodes to see the impact on energy consumption and number of hops. Simulation results show that the proposed protocol performs better than other existing techniques or in terms of parameters used in this scheme.
Water Quality Assessment and Evaluation of Human Health Risk in Mutangwi River, Limpopo Province, South Africa

Freshwater supply is essential to life on Earth; however, land use activities such as mining and agriculture pose a significant danger to freshwater resources and the wellbeing of aquatic environments. This study temporarily assesses the water quality characteristics of Mutangwi River. Physicochemical parameters (pH, temperature, total dissolved solids (TDS), salinity, electrical conductivity (EC), and turbidity) were determined in situ using an Extech multimeter and turbidity meter. The concentration of the selected metals (Mg, Cr, Fe, Cd, Mn, Pb, Ca, and Na) were analysed using an Atomic Absorption Spectrophotometer. Membrane filtration method was used to analyse microbiological parameters (Escherichia coli and Enterococci). The physicochemical water quality parameters as well as basic anions (fluoride, phosphate, sulfate, nitrate, and chloride) determined complied with the regulatory guideline of the World Health Organization (WHO) and the South Africa National Standards (SANS). Some of the trace metals (Mn, Ca, Fe, and Mg) were found below the guideline values, while others (Pb and Cd) exceeded the threshold limit. The counts for E. coli (814.5–2169 cfu/100 mL) and Enterococci (333–9396 cfu/100 mL) in the study did not comply with the regulatory guidelines. The water quality status using the water quality index (WQI) indicated that on the average, the water quality from Mutangwi River is poor (WQI > 100). The hazard quotient through ingestion exposure did not exceed the threshold limit of 1, for adults and children. This implies that there is no potential non-carcinogenic health risk from trace elements via ingestion of drinking water for children and adults. However, cancer risk for adults and children was computed in relation to Cd and Pb levels and exceeded the threshold limit 10−4, indicating a possible carcinogenic risk. Water from the river should be adequately treated prior to domestic and agricultural use.