Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell Survival in a Pathway Induced by CD74 Factor/Scatter Factor Regulate Mature B c-Met and Its Ligand Hepatocyte Growth

The signals regulating the survival of mature splenic B cells have become a major focus in recent studies of B cell immunology. Durable B cell persistence in the periphery is dependent on survival signals that are transduced by cell surface receptors. In this study, we describe a novel biological mechanism involved in mature B cell homeostasis, the hepatocyte growth factor/scatter factor (HGF)/c-Met pathway. We demonstrate that c-Met activation by HGF leads to a survival cascade, whereas its blockade results in induction of mature B cell death. Our results emphasize a unique and critical function for c-Met signaling in the previously described macrophage migration inhibitory factor/CD74-induced survival pathway. Macrophage migration inhibitory factor recruits c-Met to the CD74/CD44 complex and thereby enables the induction of a signaling cascade within the cell. This signal results in HGF secretion, which stimulates the survival of the mature B cell population in an autocrine manner. Thus, the CD74–HGF/c-Met axis deﬁnes a novel physiologic survival pathway in mature B cells, resulting in the control of the humoral immune response. The Journal of Immunology , 2010, 185: 2020–2031. from CD74 2 2 were incubated in the presence or absence of HGF (10 ng/ml) for 8 h. Total RNA was isolated, and reverse transcription using primers for Bcl-2 or HPRT was carried out. The results presented are representative of at least ﬁve different experiments. B , B220 + B cells derived from CD74 2 / 2 mice were incubated in the presence or absence of HGF (10 ng/ml) for 18 h. Cells were lysed, and levels of Bcl-2 and tubulin were analyzed by Western blot analysis. The results presented are representative of at least three separate experiments. C , B220 + B cells derived from 2 / 2 mice were incubated in the presence or absence of HGF (10 ng/ml) for 18 h. Cell death was measured by Magic Red apoptosis detection kit. The graph shows an average of the results obtained from four independent experi- ments. D , B220 + B cells derived from CD74 2 / 2 mice were incubated in the presence or absence of HGF (10 ng/ml) for 18 h. Next, B cell sub- populations were analyzed by FACS. The graph shows an average of results obtained from four independent experiments.
Harnessing Electricity in Biosystems- A Functional Tool for Tissue Engineering Applications

Tissue engineering aims to provide the necessary structural architecture and biophysical stimuli to support functioning of the target tissue. Native electricity has been demonstrated to guide the development and regeneration of a variety of tissues. Manipulation of this electricity through the external devices or the microenvironment surrounding the cell can potentially modulate function of individual cells, tissues and entire organs. This review highlights the recent progress made in utilization of electricity in tissue engineering and regenerative medicine applications.
Opinion Leaders in Star-Like Social Networks: A simple Case?

—In recent years, the automated, efﬁcient and sensitive monitoring of social networks has become increasingly important for criminal investigations and crime prevention. Previously, we have shown that the detection of opinion leaders is of great interest in forensic applications. In the present study, it is argued that state of the art opinion leader detection methods have weaknesses if networks exhibit star-like social graph topology, whereas these topologies result from the interaction of users with similar interests. This is typically the case for Facebook pages of political organizations. In these cases, the underlying topologies are highly focused on one (or only a few) central actor(s) and lead to less meaningful results by classic measures of node centrality commonly used for leader detection. The presents study examines these aspects closer and exempliﬁes them with the help of data collected from the Facebook page of a German political party for ﬁve consecutive months. Furthermore, a quantitative indicator for describing star-like network topologies is introduced and discussed. This measure can be of great value in assessing the applicability of established leader detection methods. Finally, a modiﬁed LeaderRank score is proposed – the CompetenceRank – which aims to address discussed problems.
Tumor Net (T-Net) for Classification of multi Tumors in Brain MR Images

Identification and classification of brain tumors play an incredibly significant role in patients 'medical diagnosis and treatment plans. MR Imaging modality is most preferable for Brain images due to no ionizing radiation and superior quality of the soft tissues of the Brain. The increasing quantity of magnetic imaging brain data and substantial challenges facing by traditional methods of using small data for image classification. Deep learning (DL) has become common in image classification problems in recent years and has become powerful. A deep neural network trained from scratch is proposed in this work to classify brain tumors in two publicly available datasets. The former is Brain image datasets collected from BRATS MRI brain datasets and other one is CE-MRI. The performance of the proposed Tumor Net (T-Net) is assessed with the state of art models and T-net performance is outstanding with an accuracy of 98% for BTDS-3 dataset and 99.3% for CE-MRI dataset.
ACCURACY GUARANTIES FOR (cid:2) 1 RECOVERY OF BLOCK-SPARSE SIGNALS

We introduce a general framework to handle structured models (sparse and block-sparse with possibly overlapping blocks). We discuss new methods for their recovery from incomplete observation, corrupted with deterministic and stochastic noise, using block-(cid:2) 1 regularization. While the current theory provides promising bounds for the recovery errors under a number of different, yet mostly hard to verify conditions, our emphasis is on veriﬁable conditions on the problem parameters (sensing matrix and the block structure) which guarantee accurate recovery. Veriﬁability of our conditions not only leads to efﬁciently computable bounds for the recovery error but also allows us to optimize these error bounds with respect to the method parameters, and therefore construct estimators with improved statistical properties. To justify our approach, we also provide an oracle inequality, which links the properties of the proposed recovery algorithms and the best estimation performance. Furthermore, utilizing these veriﬁable conditions, we develop a computationally cheap alternative to block-(cid:2) 1 minimization, the non-Euclidean Block Matching Pursuit algorithm. We close by presenting a numerical study to investigate the effect of different block regularizations and demonstrate the performance of the proposed recoveries.
Adam Smith’s Linen Shirt ∗

We show that the perceived quality of products, even when defined at the finest level of aggregation, changes over time. This contrasts with the standard assumptions used in the construction of price indexes, which require the quality of products to stay constant over time, at least on average. We develop a methodology to select products whose price changes are mainly driven by supply changes. We then show theoretically that this sample can be used to construct an unbiased aggregate price index. This index is the product of the conventional price index and the share of common varieties, and both components fully compensate for movements of each other. We find that previous estimates significantly underestimate the cost of living in the US.
A Novel Method for the Identification of SUMO Acceptor Lysines in a Site-Specific Manner

SUMOylation is a reversible post-translational modification essential for regulation of virtually all nuclear processes. Identification of sites of SUMOylation within proteins by mass spectrometry has been hampered by a bulky tryptic fragment, which thus far necessitated the use of mutated SUMO. Here, we present a SUMO-specific protease-based methodology which circumvents the bulky tryptic fragment altogether, dubbed Protease-Reliant Identification of SUMO Modification (PRISM). PRISM allows for detection of SUMOylated proteins as well as identifica tion of specific sites of SUMOylation while using wildtype SUMO. We successfully confirmed the validity of the methodology by purification of known SUMOylated proteins. Furthermore, PRISM was employed in combination with high-resolution mass spectrometry to identify SUMOylation sites from HeLa cells under standard growth conditions. 389 wildtype SUMOylation sites on endogenous proteins were identified, with half of these sites matching the canonical KxE consensus motif. Thus, we have developed the first methodology capable of studying wildtype SUMO at the site-specific and system-wide level. with mass spectrometry reveals 389 unique SUMOylation sites, with half adhering to the KxE consensus. lysed in M urea, buffered at pH 8.5, freshly supplemented by 10 mM chloroacetamide. Dilution to lower concentration of urea was performed using 100 mM sodium buffered at pH 8.5, freshly supplemented by 10 mM chloroacetamide. Blocking of all lysines was performed in 8 M urea buffer, by addition of SNHSA to a concentration of 20 was for 30 minutes at room catalytic domains Digestion analysis using and Trypsin digestion with per in Trypsin digestion was performed with of after diluting urea concentration to 2 M. Digestions were performed overnight at room temperature.
Modelling and analysis of the low-power 3-phase switched reluctance motor

: Switched reluctance motors (SRMs) are still under development to maximise their already proven usefulness. A magnetic circuit of the SRM can be made of soft magnetic composites (SMCs). The SMCs are composed of iron powder with dielectric and have a lot of advantages in comparison to commonly used electrical steel. The paper deals with the modellingandanalysisoftheSRMproducedbyEmersonElectricCo.forwashingmachines. Numerical calculations and modelling were done using the FEMM 4.2 program. Magnetic ﬂux densities and magnetic ﬂux lines were calculated, as well as electromagnetic torque and inductance for changing the position of a stator to a rotor. The obtained results were compared with other measurement results and are quite similar. The developed numerical model will be used for the project of a motor with an SMC magnetic circuit.
Charged Lepton Flavor Violation at the High-Energy Colliders: Neutrino Mass Relevant Particles

: We summarize the potential charged lepton ﬂavor violation (LFV) from neutrino mass relevant models, for instance the seesaw mechanisms. In particular, we study, in a model-dependent way, the LFV signals at the high-energy hadron and lepton colliders originating from the beyond standard model (BSM) neutral scalar H , doubly charged scalar H ±± , heavy neutrino N , heavy W R boson, and the Z (cid:48) boson. For the neutral scalar, doubly charged scalar and Z (cid:48) boson, the LFV signals originate from the (effective) LFV couplings of these particles to the charged leptons, while for the heavy neutrino N and W R boson, the LFV effects are from ﬂavor mixing in the neutrino sector. We consider current limits on these BSM particles and estimate their prospects at future high-energy hadron and lepton colliders.
TECHNOLOGIES AND SCOPES OF HUMAN BIOENHANCEMENT: FROM GENE EDITING TO MORAL BEHAVIOUR 1

: Issue 11 (2020) of the Ramon Llull Journal of Applied Ethics contains a monographic section in which four articles are published on human enhancement. Two of them (those by Alonso, Anomaly & Savulescu, and Capasso & Santoema) deal with the use of CRISPR gene editing systems for the enhancement of human capabilities. The other two (those of Conradie and Rueda) deal with different ethical aspects of using biotechnology to increase human morality. In the present article, F. Lara and M. Moreno contextualize these four contributions by pointing out the background and inter-pretative keys to the debates in which they are inserted.
Left Ventricular Dimensions and Autonomic Balance During Head-Up Tilt Differ Between Patients With Isoproterenol-Dependent and Isoproterenol-Independent Neurally Mediated Syncope

Objectives. This study sought to elucidate differences in mechanisms of neurally mediated syncope between patients with syncope induced by head-up tilt alone and those requiring isoproterenol infusion to induce syncope during head-up tilt. Background. Some patients with neurally mediated syncope require isoproterenol to induce syncope during head-up tilt (isoproterenol dependent), and others do not (isoproterenol independent). Differences in mechanisms between these two groups have not been well elucidated. Methods. A 60* head-up tilt test was performed in 13 patients with isoproterenoMndependent syncope (Group I, mean [_+SD] age 28 -+ 12 years), 14 patients with isoproterenol-dependent syncope (Group II, mean age 34 _+ 14 years) and 20 control subjects without syncope (Group III, mean age 32 -+ 12 years). Left ventricular size and contractility were determined by echocardiography, and sympathovagal balance was determined with power spectral analysis of heart rate variability using a maximal entropy method. Results. Group I patients had smaller left ventricular dimensions than Groups II and III during baseline tilt. During head.up tilt with isoproterenoi infusion (0.01 to 0.04/xg/kg body weight per min), left ventricular dimensions decreased to the same extent in Groups II and III, but fractional shortening was greater in Group II than in Group III at the end of the tilt. The ratio of low (0.05 to 0.15 Hz) to high frequency (0.15 to 1.0 Hz) component became greater in Group I than in Groups H and III during the last period of baseline tilt. However, the ratio was greater in Group II than in Group III during the last period of the tilt with isoproterenol. Conclusions. Patients with isoproterenoMndependent syncope had an exaggerated decrease in left ventricular size and sympathetic predominance preceding syncope during head-up tilt. In contrast, in patients with isoproterenol-dependent syncope, similar changes in autonomic nervous balance were evident only during isoproterenol infusion in addition to head-up tilt. (J Am Coil Cardiol 1995;26:164-73)
AGROBACTERIUM-MEDIATED TRANSFORMATION OF SORGHUM

The first reports on Agrobacterium-mediated transformation of monocotyledonous were controversial because they provided neither convincing evidence of a stable integration of the transgenes into the plant genome nor of Mendelian segregation of transgenes in the progeny (Vasil, 1994). Recently researchers from Japan Tobacco Inc. reported the first irrefutable proof that Agrobacterium could be used to transform rice (Hiei et al. 1994). They produced a large number of transgenic plants and showed stable integration of the transgenes. Since then many other rice genotypes have been transformed (Aldemita and Hodges 1996, Rashid et al. 1996, Toki 1997, Wang et al. 1997) and the technique has been extended to maize (Ishida et al. 1996), barley (Tingay et al. 1997) and wheat (Cheng et al. 1997). There are three reports that describe stable transformation of sorghum plants (Casas et al. 1993, 1997; Zhu et al. 1998) by particle bombardment using immature embryos or inflorescence. Although transgenic plants were produced the transformation efficiency was low. The selectable marker used during the transformation procedure was the bar gene, a gene known to confer resistance to the commercial herbicide Basta. The use of the bar gene presents a potential problem because transformed sorghum plants could cross with some wild relatives and transfer the herbicide resistance trait to weeds. On the other hand, there is no report of Agrobacterium-mediated transformation of sorghum. Agrobacterium-mediated transformation has some significant advantages over other methods of transformation. These include the transfer of relatively large segments of DNA with defined ends, integration of a lower number of gene copies into plant chromosomes, and the absence of a requirement for protoplast culture (reviewed by Vasil, 1994). Furthermore, Agrobacterium-mediated transformation is much more efficient in simultaneously producing events with lower gene copy number and simpler insertion patterns than genes inserted by particle bombardment (Zuo-yo Zhao, personal communication, Pioneer HiBred International). Work reported here demonstrates that Agrobacterium-mediated transformation of sorghum is feasible. Several factors that influenced transformation efficiency, such as sensitivity of sorghum explants to Agrobacterium infection, explant type, inoculation method and co-cultivation media, are discussed. The sensitivity of immature embryos to the Agrobacterium infection was considered the limiting step to increasing the transformation efficiency. GUS expression was evaluated as a tool for monitoring the transformation events and is shown to be expressed only in specific tissues in sorghum under the conditions of this study.
DMD # 86397 CLEAN ALL TEXT Figs TABLES 010319

Current affiliation FH Aachen, University of Applied Sciences, Heinrich-Mußmann-Str.1, 52428 Jülich, Germany (NS); F. Hoffmann-La Roche, Grenzacherstrasse 1244070 Basel, Switzerland (AR); Concept Life Sciences, Dundee Technopole, 2 James Lindsay Place, Dundee, DD1 5JJ (KM); Drug Discovery Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH (DL). This article has not been copyedited and formatted. The final version may differ from this version. DMD Fast Forward. Published on March 25, 2019 as DOI: 10.1124/dmd.119. 086397
Mutation analysis of cell-free DNA and single circulating tumor cells in metastatic breast cancer patients with high CTC counts

Purpose: The purpose of this study was to directly compare mutation profiles in multiple single CTCs and cfDNA isolated from the same blood samples taken from patients with metastaic breast cancer (MBC). We aimed to determine whether cell-free DNA would reflect the heterogeneity observed in 40 single CTCs. Experimental design: CTCs were enumerated by Cellsearch. CTC count was compared with the quantity of matched cfDNA and serum CA15-3 and alkaline phosphatase (ALP) in 112 patients with metastatic breast cancer. In 5 patients with ≥100 CTCs, multiple individual EpCAM-positive CTCs were isolated by DEPArray and compared with matched cfDNA and primary tumour tissue by targeted next generation sequencing (NGS) of ~2200 mutations in 50 cancer genes. Results: In the whole cohort, total cfDNA levels and cell counts (≥5 CTCs) were both significantly associated with overall survival, unlike CA15-3 and ALP. NGS analysis of 40 individual EpCAM-positive CTCs from 5 patients with MBC revealed mutational heterogeneity in PIK3CA, TP53, ESR1 and KRAS genes between individual CTCs. In all 5 patients cfDNA profiles provided an accurate reflection of mutations seen in individual CTCs. ESR1 and KRAS gene mutations were absent from primary tumour tissue and therefore likely reflect either a minor sub-clonal mutation or were acquired with disease progression. Conclusion: Our results demonstrate that cfDNA reflects persisting EpCAM-positive CTCs in patients with high CTC counts and therefore may enable monitoring of the metastatic burden for clinical decision-making. Next generation sequencing is a key approach for monitoring tumour genomic alterations in circulating cell free DNA (cfDNA). High circulating tumor cells (CTCs) count (≥5 EpCAM-positive CTCs per 7.5ml blood as measured by Cellsearch) is a poor prognostic indicator, but thus far individual CTCs have not been directly compared with matched cfDNA by mutation profiling. However, concurrent analysis of individual CTCs and matched cfDNA has the potential for comprehensive characterisation of tumour derived genetic alterations in blood by“liquid biopsy”. In this article we show that individual CTCs have heterogeneous mutations, and that cfDNA isolated from the same blood sample provides an accurate reflection of mutations seen in individual CTCs. We also show that the total cfDNA level, like CTC counts, is an independent prognostic marker in patients with metastatic breast cancer. Overall, our results suggest that cfDNA reflects persisting EpCAM-positive CTCs in patients with high CTC counts and could potentially enable monitoring of the metastatic burden for clinical decision-making when CTCs cannot be obtained.
4.0. Performance, blood chemistry, carcass and economic evaluation of sheep on dietary supplements of cabbage and carrot

https://doi.org/10.30574/wjarr.2021. 12.2. 0615 Abstract The study was conducted to determine the effect of cabbage and carrot wastes supplementation on performance, blood chemistry, carcass and economic evaluation of sheep. Twenty four West African dwarf male sheep of about 8 – 9 months old with average initial weight of 9.00 ± 0.94kg were randomly allotted to four dietary treatments with six sheep per treatment, in a completely randomized design. The sheep received 60% guinea grass and 40% concentrate diet for TD 1 (control group), while 50% guinea grass and 40% concentrate diet with either 10% cabbage waste, 5% cabbage plus 5% carrot wastes or 10% carrot waste were for diets TD 2 , TD 3 and TD 4 respectively. The results showed that sheep on TD 1 were significantly (p<0.05) higher in feed conversion ratio, creatinine, urea, rumen with intestine and total fixed cost. Diet TD 2 was significantly (p<0.05) improved in daily weight gain, digestibility of dry matter, crude protein with fiber and nitrogen free extract, haemoglobin, packed mean corpuscular blood cell, profit. Red blood cell, cholesterol and triglyceride were increased significantly (p<0.05) in TD 3, while daily feed intake, digestibility of ash, feed cost and total variable cost were better in TD 4 . Meanwhile, no significant difference (p>0.05) was found in initial body weight, ether extract digestibility, mean corpuscular volume, mean corpuscular haemoglobin concentration, monocyte, eosinophil, basophil, globulin and some relative organs weight among sheep on treatments. It can be concluded that inclusion level of 10% cabbage waste as supplement to 50% guinea grass and 40% concentrate diet (TD 2 ) improved performance, health status and carcass indices of sheep with reduced cost that resulted in higher net
Antisotropic Cosmological Models of Finsler Space With ( γ, β ) -Metric

: In this paper we have studied an anisotropic model of space time with Finslerian spaces with ( γ, β )-metrics as suggested by one of the co-author in his paper [21] with an extra requirement of γ 3 = a ijk ( x ) y i y j y k . Here γ , is a cubic metric and β = b i ( x ) y i , is a one form metric. The observed anisotropy of the microwave background radiation is incorporated in the Finslerian metric of space time.
Stabilityand reproducibility of low-temperature anaerobic biological wastewater treatment

The reproducibility of low-temperature anaerobic biological wastewater treatment trials was evaluated. Two identical anaerobic expanded granular sludge bed bioreactors were used to treat synthetic volatile fatty acid-based industrial wastewater under ambient conditions (18–20 1 C) and to investigate the effect of various environmental perturbations on reactor performance and microbial community dynamics, which were assessed by chemical oxygen demand removal or efﬂuent volatile fatty acid determination and terminal restriction fragment length polymorphism analysis, respectively. Methanogenic activity was monitored using speciﬁc methanogenic activity assays. Reactor performance and microbial community dynamics were each well replicated between Reactor 1 and Reactor 2. Archaeal dynamics, in particular, were associated with reactor operating para-meters. Terminal restriction fragment length polymorphism data suggested dynamic acetoclastic and hydrogenophilic methanogenic populations and were in agreement with temporal speciﬁc methanogenic activity data. Putative psychrophilic populations were observed in anaerobic bioreactor sludge for the ﬁrst time.