Miracle-dz/UniFAR-FTUs huggingface dataset:
Atrazine‐induced alterations in rat erythrocyte membranes: Ameliorating effect of vitamin E

Erythrocytes are a convenient model to understand oxidative damage to the membranes induced by various xenobiotics. The objective of the present study was to investigate the propensity of atrazine to induce oxidative stress and its possible attenuation by vitamin E. Experimental animals were orally administered atrazine (300 mg kg−1 body weight, daily) and vitamin E (100 mg kg−1 body weight, daily) for a period of 7, 14, and 21 days. Erythrocyte membranes were prepared and analyzed for acetylcholinesterase (AChE) activity, lipid peroxidation (LPO), and lipid composition. Susceptibility of erythrocytes to atrazine exposure was further investigated in terms of morphological alterations by scanning electron microscopy (SEM). Results indicate that atrazine exposure caused a significant inhibition of AChE activity and induction of oxidative stress in terms of increased malondialdehyde (MDA) levels. Atrazine treatment significantly decreased total lipid, cholesterol, and phospholipid content of erythrocyte membranes. SEM revealed varying degrees of distortion depending on duration of atrazine exposure. However, administration of vitamin E ameliorated the oxidative stress and changes in the erythrocyte membranes induced by atrazine. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:363–369, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20249
On Modal Logic Interpretation of Possibility Theory

This paper is a continuation in a series of papers started by Resconi et. al. [8] in which we try to develop interpretations of various uncertainty theories within the framework of standard modal logic. In this paper, we deal with possibility theory. We suggest an interpretation of possibility measures and necessity measures and show that this interpretation is complete for rational-valued measures.
Epidémiologie moléculaire et métagénomique à haut débit sur la grille. (Molecular epidemiology and high-throughput metagenomics on the grid)

The objective of this thesis focuses on the study and the development of bioinformatics platforms and tools on the grid. The second objective is to develop applications in molecular epidemiology and metagenomics based on these tools and platforms. Based on the studies of existing bioinformatics platforms and tools, we propose our solution: a platform and a portal for molecular epidemiology and high throughput metagenomics on the grid. The main idea of ​​our platform is to simplify the submission of jobs to the grid via the pilots jobs (jobs generic that can control and launch many real tasks) and the PULL model (tasks are retrieved and executed automatically). There are other platforms that have similar approaches but our platform focuses on the simplicity and the saving time for the submission of jobs. Bioinformatics tools chosen to deploy the platform are popular tools that can be used in many bioinformatics analyses. We apply a workflow engine in the platform so that users can make the analysis easier. Our platform can be seen as a generalized system that can be applied to both the epidemiological surveillance and metagenomics of which two use cases are deployed and tested on the grid. The first use case is used to monitor bird flu. The approach of this application is to federate data sequences of influenza viruses and provide a portal with tools on the grid to analyze these data. The second use case is used to apply the power of the grid in the analysis of high throughput sequencing of amplicon sequences. In this case, we prove the efficiency of the grid by using our platform to gridifier an existing application, which has much less performance than the gridified version.
Spatially Resolved Plant Metabolomics: Some Potentials and Limitations of Laser-Ablation Electrospray Ionization Mass Spectrometry Metabolite Imaging1[OPEN]

Laser-ablation electrospray ionization mass spectrometry imaging of living plant tissues gives deeper insights into the heterogenic and dynamic nature of metabolite accumulation in plant organs. Laser-ablation electrospray ionization (LAESI)-mass spectrometry imaging has been applied to contrasting plant organs to assess its potential as a procedure for performing in vivo metabolomics in plants. In a proof-of-concept experiment, purple/white segmented Phalaenopsis spp. petals were first analyzed using standard liquid chromatography-mass spectrometry analyses of separate extracts made specifically from the purple and white regions. Discriminatory compounds were defined and putatively annotated. LAESI analyses were then performed on living tissues, and these metabolites were then relocalized within the LAESI-generated data sets of similar tissues. Maps were made to illustrate their locations across the petals. Results revealed that, as expected, anthocyanins always mapped to the purple regions. Certain other (nonvisible) polyphenols were observed to colocalize with the anthocyanins, whereas others were found specifically within the white tissues. In a contrasting example, control and Cladosporium fulvum-infected tomato (Solanum lycopersicum) leaves were subjected to the same procedures, and it could be observed that the alkaloid tomatine has clear heterogeneous distribution across the tomato leaf lamina. Furthermore, LAESI analyses revealed perturbations in alkaloid content following pathogen infection. These results show the clear potential of LAESI-based imaging approaches as a convenient and rapid way to perform metabolomics analyses on living tissues. However, a range of limitations and factors have also been identified that must be taken into consideration when interpreting LAESI-derived data. Such aspects deserve further evaluation before this approach can be applied in a routine manner.
Leukocyte-Endothelium Interactions during Permanent Focal Cerebral Ischemia in Mice

The contribution of leukocyte infiltration to brain damage after permanent focal cerebral ischemia and the underlying molecular mechanisms are still unclear. Therefore, the aim of this study was to establish a mouse model for the visualization of leukocytes in the cerebral microcirculation in vivo and to investigate leukocyte-endothelial interaction (LEI) after permanent middle cerebral artery occlusion (MCAO). Sham-operated 129/Sv mice showed physiologic LEI in pial venules as observed by intravital fluorescent microscopy. Permanent focal cerebral ischemia induced a significant increase of LEI predominantly in pial venules. The number of rolling and adherent leukocytes reached 36.5 ± 13.2/100 μm × min and 22.5 ± 7.9/100 μm × min, respectively at 120 minutes after MCAO (P = 0.016 vs. control). Of note, rolling and adherent leukocytes were also observed in arterioles of ischemic animals (7.3 ± 3.0/100 μm × min rolling and 3.0 ± 3.6/100 μm × min adherent). Capillary density was not different between groups. These results demonstrate that leukocytes accumulate in the brain not only after transient but also after permanent focal cerebral ischemia and may therefore contribute to brain damage after stroke without reperfusion.
Median-Truncated Nonconvex Approach for Phase Retrieval With Outliers

This paper investigates the phase retrieval problem, which aims to recover a signal from the magnitudes of its linear measurements. We develop statistically and computationally efficient algorithms for the situation when the measurements are corrupted by sparse outliers that can take arbitrary values. We propose a novel approach to robustify the gradient descent algorithm by using the sample median as a guide for pruning spurious samples in initialization and local search. Adopting a Poisson loss and a reshaped quadratic loss, respectively, we obtain two algorithms termed median-truncated Wirtinger flow and median-reshaped Wirtinger flow, both of which provably recover the signal from a near-optimal number of measurements when the measurement vectors are composed of independent and identically distributed Gaussian entries, up to a logarithmic factor, even when a constant fraction of the measurements is adversarially corrupted. We further show that both algorithms are stable in the presence of additional dense bounded noise. Our analysis is accomplished by developing non-trivial concentration results of median-related quantities, which may be of independent interest. We provide numerical experiments to demonstrate the effectiveness of our approach.
Vibrio cholerae Hcp, a secreted protein coregulated with HlyA

Hcp is a 28-kDa secreted protein of Vibrio cholerae regulated coordinately with the hemolysin, HlyA. Both proteins show a dependence on HlyU for expression, suggesting that Hcp may be secreted by V. cholerae in vivo. We have identified and sequenced two genes for Hcp, designated hcpA and hcpB (hemolysin-coregulated protein). The genes encode identical amino acid sequences. Both express a 28-kDa protein, despite open reading frames with only a 19-kDa capacity, suggesting that the Hcp protein runs aberrantly on polyacrylamide gel electrophoresis. There is no cleavage involved in secretion of Hcp from the cell, suggesting a novel mechanism of secretion. An hcp null mutant was constructed, and this strain displayed no deficiency in virulence or colonization in the infant mouse cholera model. From sequence data and primer extension analysis, we predict that the hcp promoter is the sigma 54 type, with a candidate integration host factor binding site upstream. Although hcp and hlyA are coregulated by HlyU, there are no obvious similarities between their promoters. We predict that an intermediate regulator may be involved in the activation of hcp by HlyU. This raises the possibility that HlyU is part of a regulatory cascade.
Arista Networks——开创网络新纪元

数据中心正在向万兆以太网技术过渡，以此来满足当今网络爆炸性增长的数据需求。 随着万兆以太网成为市场的亮点，新一代的数据中心级产品也逐渐显示出其竞争力。 初创公司Arista Networks研制的用于计算机数据中心的超速网络交换机，目前正在这一市场飞速成长，它在体系架构、可靠性、延迟性等多个方而实现了飞跃性的突破，树立了交换领域的新标杆。
Stator winding fault diagnosis in induction motors using the dq current trajectory mass center

This paper proposes the use of the park transformation mass center applied to the stator currents as a method for diagnosing the occurrence of stator winding faults in induction motor. Induction motor stator currents are first measured and recorded. Then, the park transform is applied to the obtained currents in order to obtain a specific pattern that allows the identification of the stator winding fault. For a healthy motor, a single point in a dq-plane is obtained. However, for an induction motor with some stator winding fault one obtains a circle, in the dq-plane, which is dependent on the fault severity. Accordingly to this relationship a fault severity is reported. In order to show the applicability of the proposed technique, several simulation and experimental results are presented.
An Energy Efficient Secure Logic to Provide Resistance against Differential Power Analysis Attacks

In this article, we propose a new logic style which is resistant to Differential Power Analysis (DPA) attacks as well as energy efficient, to provide security to devices like smart cards. The main drawback in the existing secure logic styles was the high power consumption which is reduced significantly by using adiabatic approach. Dual-rail logic is used to maintain security and adiabatic logic is combined with it to reduce the power consumption. These ideas are implemented using Hspice tool, a significant improvement in power consumption and resistance against DPA attacks are observed when compared to the existing logic styles.
The Role of Tropical Waves in Tropical Cyclogenesis

Abstract This paper analyzes relationships between tropical wave activity and tropical cyclogenesis in all of the earth’s major tropical cyclone basins. Twenty-nine years of outgoing longwave radiation data and global reanalysis winds are filtered and analyzed to determine statistical relationships between wave activity in each basin and the corresponding cyclogenesis. Composite analyses relative to the storm genesis locations show the structures of the waves and their preferred phase relationships with genesis. Five wave types are examined in this study, including mixed Rossby–gravity waves, tropical-depression-type or easterly waves, equatorial Rossby waves, Kelvin waves, and the Madden–Julian oscillation. The latter is not one of the classical tropical wave types, but is a wavelike phenomenon known to have a strong impact on tropical cyclogenesis. Tropical cyclone formation is strongly related to enhanced activity in all of the wave filter bands except for the Kelvin band. In each basin the structure o.
Space-time block coding in correlated channels

We propose an approach to the problem of constructing linear space-time block-codes that provides a unified treatment to all such codes at any code rate and any number of transmits and receives antennas. The analysis is carried out by considering the bound on the probability of error between any two possible blocks of incoming data, under the constraint that the average transmitted power from each antenna at each time should be equal. We consider both the case of a independent identically distributed channel coefficients, and the case of a correlated low-rank channel.
Purification and identification of sperm surface proteins and changes during epididymal maturation

Surface membrane proteins have a key role in the sequential interactions between spermatozoa and oocytes. The aim of this study was to characterize protein changes occurring during post‐testicular differentiation using a new overall approach to study surface membrane proteins of spermatozoa. A dedicated protocol based on specific purification of surface membrane proteins labeled with sulfo‐NHS‐SS‐biotin was developed for this purpose. Appropriate gel electrophoresis separation and purification methods combined with standard proteomic methods were then used to identify and quantify surface membrane proteins from immature and mature spermatozoa. Membrane‐associated proteins were discriminated from integral membrane proteins by differential solubilization. Protein regionalization on the spermatozoon surface was achieved by comparative analysis of the surface protein extracts from the entire spermatozoa and from periacrosomal sperm plasma membranes. Identification of several known proteins and of new proteins related to the process of epididymal maturation showed the reliability of this protocol for specific purification of a subproteome and identification of new sperm membrane proteins. This approach opens up a new area in the search for male fertility markers.
The Relationship between Antioxidant and Anti-Ulcer Activities in Saudi Honey Samples Harvested from Various Regions in Different Seasons

The main chemical components of 13 Saudi honey samples (composed of winter and summer honeys) were identified according to anti-ulcer and antioxidant activity by using phytochemical and chromatographic analyses. Phytochemical screening of ethyl acetate and water extracts was used to detect the presence of carbohydrates, flavonoids, amino acids, and phenolic acids. High-performance liquid chromatography (HPLC) of sugar contents was used to detect the presence of galactose in all of the honey samples. The other detected sugars were sucrose, fructose, and arabinose. Fifteen amino acids were detected in all of the honey samples: Prolin is dominant in all of the honey samples. The citric acid and tartaric acids of winter and summer honey were also detected, in addition to faint traces of free oxalic acid. Both samples were tested for amino acids, phenolic compounds, and sugars. The most crucial result derived in this study is the effect of summer honey on ulcers. The anti-ulcer activities were also evaluated, showing that oral administration of the honey samples reduced the intensity of ulcer scores when compared to the control group. Similarly, there was a highly significant reduction in the values of the ulcer indices and areas in rats that received the same sample (P < 0.05 and P < 0.01) in comparison with those of the ulcer control rats. Finally, the antioxidant activity of the honey samples was evaluated, revealing a proportional relationship between the anti-oxidant and anti-ulcer activities. The results of this study could be reached that the effectiveness of honey as an anti-ulcer increase with the increasing its antioxidant activities.
Improved Genetic Algorithm-Based Resource Scheduling Strategy in Cloud Computing

In this paper, combined the characteristics of cloud computing, we propose a consider to Time and Cost constraints Genetic Algorithm (TCGA), the algorithm is used to complete the task completion time and the total task completion cost as the standard, it for resource scheduling. The results show that: You can achieve a more reasonable resource scheduling in the cloud computing platform through this algorithm, and produce the desired task scheduling result.
Promoting Adaptive Emotion Regulation and Coping in Adolescence

Particularly in adolescence, fostering adaptive emotion regulation is an important aim in health promotion. Expressive writing in combination with psycho-education on emotion regulation seems especially appropriate to serve this aim. In this study, school classes were randomly assigned either to a prevention (N = 208) or to a non-treatment control group (N = 151). The prevention group showed significant improvements regarding negative affect, grades, and days absent compared to the control-group. A combination of expressive writing with elements of psycho-education of emotion regulation might be an effective preventive tool, as it seems to improve psychosocial adjustment by establishing functional emotion regulation strategies.
Characterization of HscC (Hsc62), homologue of Hsp70 in Escherichia coli: over‐expression of HscC modulates the activity of house keeping sigma factor σ70

Background: HscC, the third member of the Hsp70 family in Escherichia coli, shares 33% identity with the other two homologues, DnaK and HscA, and displays ATPase activity. Genetic and biochemical evidence indicates that the DnaK‐DnaJ chaperone system interacts with σ32 and is involved in the negative regulation of the heat shock response. Although HscC is a highly conserved protein in the Hsp70 family, its function is still unknown.
Carbapenemase-producing Enterobacteriaceae: a call for action!

Since the beginning of this century, we have been observing increasingly rapid diffusion among Enterobacteriaceae of extended-spectrum b-lactamases (ESBLs), mostly of the CTX type and mostly in Escherichia coli [1]. This phenomenon has run out of control within just 10 years, and the outbreak is now internationally identified as being, in particular, a source of community-acquired infections. In this context of multiresistance, the increasing number of carbapenemase-producing Enterobacteriacae is worrying, because carbapenems represent the last line of antibiotics that are still effective for treating many enterobacterial infections [2,3]. Although the hydrolytic profile of carbapenemases (mostly KPC, OXA-48, IMP, VIM, and NDM) is somewhat variable, all of them hydrolyse at least carbapenems [4–6]. The corresponding genes may be considered as markers of multidrug resistance—or even pandrug resistance—in specific areas in the world, as they are often found together with genes conferring resistance to most b-lactams, aminoglycosides, and fluoroquinolones. All of these genes are easily transferable within Enterobacteriaceae, because they are plasmid-borne. If one considers the carbapenemase type and the host bacterial species, so far KPC, IMP and VIM seem to have been mostly identified in the main nosocomial pathogen, which is Klebsiella pneumoniae [4–6]. By contrast, in the case of carbapenemases of the NDM and OXA-48 types, both K. pneumoniae and E. coli can be regarded as sources of both nosocomial and community-acquired infections [4–6]. Identification of carbapenemase producers in Enterobacteriacae is becoming the major issue among antibiotic resistance problems worldwide, and in particular in Europe, for the following reasons:
Anti-inflammatory activity in oak gall extracts.

A method for the purification of the Hungarian oak gall extracts containing anti-inflammatory activity is described. The anti-inflammatory activity of the purified extract was tested in the carrageenan-induced rat paw edema and in rat polyarthritis induced by mycobacterial adjuvant. In doses of 4.3 and 8.5 mg/kg i.p., the extract inhibited dose-dependently the formation of paw edema induced by carrageenan in rats. When rats were treated daily from 3 days prior to and 16 days after the injection of the mycobacterial adjuvant, the severity of the polyarthritic symptoms was significantly reduced.
Aquatic aerobic exercise for children with cerebral palsy: a pilot intervention study

Abstract Purpose: The primary purpose of this pilot study was to evaluate the effectiveness of a14-week aquatic exercise program on gross motor function and walking endurance in children with cerebral palsy (CP). The secondary purpose was to evaluate changes in functional strength, aerobic capacity and balance. Method: A prospective time series group design consisting of four measurement sessions (two baseline, one post intervention, and 1-month follow-up) was used. Eight ambulatory children ages 6–15 years with CP and classified at Gross Motor Function Classification System Level I or Level III participated in an aquatic aerobic exercise program. Results: Significant improvements were observed for the primary outcomes of gross motor function and walking endurance. No significant differences between any of the secondary measures were observed, although all of the measures demonstrated trends of improvement after intervention. Conclusion: Ambulatory children with CP may improve their gross motor skills and walking endurance after an aquatic exercise program held twice per week for 14 weeks, utilizing moderate-to-vigorous exercise intensity and consisting of functional activities.
Differential dendritic targeting of AMPA receptor subunit mRNAs in adult rat hippocampal principal neurons and interneurons

In hippocampal neurons, AMPA receptors (AMPARs) mediate fast excitatory postsynaptic responses at glutamatergic synapses, and are involved in various forms of synaptic plasticity. Dendritic local protein synthesis of selected AMPAR subunit mRNAs is considered an additional mechanism to independently and rapidly control the strength of individual synapses.
VALIDATION OF HPLC METHOD FOR DETERMINATION OF THIAMINE HYDROCHLORIDE, RIBOFLAVIN, NICOTINAMIDE, AND PYRIDOXINE HYDROCHLORIDE IN SYRUP PREPARATION

Multivitamin syrup containing various substances of varying characteristics may have a problem in quantitative analysis. This research has developed and validated of HPLC method for determination of four vitamin components, that is thiamine hydrochloride, riboflavin, nicotinamide, and pyridoxine hydrochloride in syrup multivitamin-mineral. The chromatographic separation was achieved by using a C18 column with dimension of 3.9x300mm and particle size of 10µm. A mixture of methanol – 1% acetic acid by using 7mM 1-hexane sulphonic acid sodium salt (20:80) as mobile phase with flow rate of 1mL/min. The effluent was monitored at 280 nm. Effective separation and quantification was achieved in less than 20 min. That method was simple, accurate, precise, and could be successfully applied for the analysis of thiamine hydrochloride, riboflavin, nicotinamide, and pyridoxine hydrochloride syrup multivitamin-mineral.
Validation of an Antimicrobial Stewardship-Driven Verigene Blood-Culture Gram-Negative Treatment Algorithm to Improve Appropriateness of Antibiotics

Abstract Rapid diagnostic testing (RDT) allows for early adjustment of antibiotic therapy. This study examined the potential impact of a stewardship-driven antibiotic treatment algorithm, incorporating RDT into the management of Gram-negative bacteremia. The proposed algorithm would have resulted in 88.4% of cases receiving appropriate antibiotic therapy versus 78.1% by standard of care (P = .014).