Miracle-dz/UniFAR-FTUs huggingface dataset:
Inter-species cortical registration between macaques and humans using a functional network property under a spherical demons framework

Systematic evaluation of cortical differences between humans and macaques calls for inter-species registration of the cortex that matches homologous regions across species. For establishing homology across brains, structural landmarks and biological features have been used without paying sufficient attention to functional homology. The present study aimed to determine functional homology between the human and macaque cortices, defined in terms of functional network properties, by proposing an iterative functional network-based registration scheme using surface-based spherical demons. The functional connectivity matrix of resting-state functional magnetic resonance imaging (rs-fMRI) among cortical parcellations was iteratively calculated for humans and macaques. From the functional connectivity matrix, the functional network properties such as principal network components were derived to estimate a deformation field between the human and macaque cortices. The iterative registration procedure updates the parcellation map of macaques, corresponding to the human connectome project’s multimodal parcellation atlas, which was used to derive the macaque’s functional connectivity matrix. To test the plausibility of the functional network-based registration, we compared cortical registration using structural versus functional features in terms of cortical regional areal change. We also evaluated the interhemispheric asymmetry of regional area and its inter-subject variability in humans and macaques as an indirect validation of the proposed method. Higher inter-subject variability and interhemispheric asymmetry were found in functional homology than in structural homology, and the assessed asymmetry and variations were higher in humans than in macaques. The results emphasize the significance of functional network-based cortical registration across individuals within a species and across species.
Klotho deficiency intensifies hypoxia-induced expression of IFN-α/β through upregulation of RIG-I in kidneys

Hypoxia is a common pathway to the progression of end-stage kidney disease. Retinoic acid-inducible gene I (RIG-I) encodes an RNA helicase that recognizes viruses including SARS-CoV2, which is responsible for the production of interferon (IFN)-α/β to prevent the spread of viral infection. Recently, RIG-I activation was found under hypoxic conditions, and klotho deficiency was shown to intensify the activation of RIG-I in mouse brains. However, the roles of these functions in renal inflammation remain elusive. Here, for in vitro study, the expression of RIG-I and IFN-α/β was examined in normal rat kidney (NRK)-52E cells incubated under hypoxic conditions (1% O2). Next, siRNA targeting RIG-I or scramble siRNA was transfected into NRK52E cells to examine the expression of RIG-I and IFN-α/β under hypoxic conditions. We also investigated the expression levels of RIG-I and IFN-α/β in 33 human kidney biopsy samples diagnosed with IgA nephropathy. For in vivo study, we induced renal hypoxia by clamping the renal artery for 10 min in wild-type mice (WT mice) and Klotho-knockout mice (Kl-/- mice). Incubation under hypoxic conditions increased the expression of RIG-I and IFN-α/β in NRK52E cells. Their upregulation was inhibited in NRK52E cells transfected with siRNA targeting RIG-I. In patients with IgA nephropathy, immunohistochemical staining of renal biopsy samples revealed that the expression of RIG-I was correlated with that of IFN-α/β (r = 0.57, P<0.001, and r = 0.81, P<0.001, respectively). The expression levels of RIG-I and IFN-α/β were upregulated in kidneys of hypoxic WT mice and further upregulation was observed in hypoxic Kl-/- mice. These findings suggest that hypoxia induces the expression of IFN-α/β through the upregulation of RIG-I, and that klotho deficiency intensifies this hypoxia-induced expression in kidneys.
The complete chloroplast genome and phylogenetic analysis of Potentilla sischanensis Bunge ex Lehm

Abstract Potentilla sischanensis Bunge ex Lehm. is a widespread perennial herb in north China. The plant has little yellow flowers, and the petioles are white-tomentose and sparsely villous. To determine the chloroplast genome, total genomic DNA was extracted from fresh leaves and sequenced. The complete chloroplast genome was assembled and annotated. The chloroplast genome of this plant is a circular form with a length of 156,240 bp, including a large single-copy region (LSC, 85,748 bp), a small single-copy region (SSC, 18,566 bp), and two inverted repeats (IRs, 25,963 bp). A total of 132 genes were predicted, comprising 87 encoded proteins, 8 rRNAs and 37 tRNAs. The evolutionary history indicates that P. sischanensis was grouped within Potentilla and formed a clade with Potentilla chinensis and Potentilla stolonifera with a 100% bootstrap support value. The complete cp genome can serve as a reference for future studies on molecular biology, evolution, population genetics, taxonomy and resource protection.
A randomized double-blind placebo-controlled trial of the effectiveness of melatonin on neurocognition and sleep in survivors of childhood cancer.

BACKGROUND Adult survivors of childhood cancer are at risk of developing sleep and neurocognitive problems, yet few efficacious interventions exist targeting these prevalent late effects. Melatonin has known sleep-promoting effects; however, it has not been well studied among childhood cancer survivors. METHOD Survivors (n = 580; mean age = 33.5 years; 26 years post-diagnosis) from the St. Jude Lifetime Cohort were randomized (1:1) to a six-month double-blind placebo-controlled trial of 3 mg time-release melatonin within three strata (stratum 1: neurocognitive impairment only; stratum 2: neurocognitive and sleep impairment; stratum 3: sleep impairment only). Neurocognitive performance was assessed at baseline and post-intervention using standardized measures. Sleep was assessed via self-report and actigraphy. Independent sample t tests compared mean change scores from baseline to six months. Post-hoc analyses compared the prevalence of clinically significant treatment responders among melatonin and placebo conditions within and across strata. RESULTS Intent-to-treat analyses revealed no statistically significant differences in neurocognitive performance or sleep from baseline to post-intervention. However, among survivors with neurocognitive impairment only, a larger proportion randomized to melatonin versus placebo demonstrated a treatment response for visuomotor speed (63% vs 41%, P = 0.02) and nonverbal reasoning (46% vs 28%, P = 0.04). Among survivors with sleep impairment only, a larger proportion treated with melatonin demonstrated a treatment response for shifting attention (44% vs 28%, P = 0.05), short-term memory (39% vs 19%, P = 0.01), and actigraphy-assessed sleep duration (47% vs 29%, P = 0.05). CONCLUSION Melatonin was not associated with improved neurocognitive performance or sleep in our intent-to-treat analyses; however, a subset of survivors demonstrated a clinically significant treatment response.
Open surgery for in utero repair of spina bifida: Microneurosurgery versus standard technique – A systematic review

Prenatal myelomeningocele (MMC) repair has been shown to improve neurological outcomes. It has been suggested that decreases in the hysterotomy diameter during surgery can improve perinatal outcomes without altering neurologic outcomes. The objective of this study is to describe and compare the main maternal and fetal outcomes of fetuses undergoing open surgery for MMC repair, through the different modifications (standard‐classical, mini‐hysterotomy, and microneurosurgery).
Millimeter-Wave-Based Spoof Localized Surface Plasmonic Resonator for Sensing Glucose Concentration

Glucose-monitoring sensors are necessary and have been extensively studied to prevent and control health problems caused by diabetes. Spoof localized surface plasmon (LSP) resonance sensors have been investigated for chemical sensing and biosensing. A spoof LSP has similar characteristics to an LSP in the microwave or terahertz frequency range but with certain advantages, such as a high-quality factor and improved sensitivity. In general, microwave spoof LSP resonator-based glucose sensors have been studied. In this study, a millimeter-wave-based spoof surface plasmonic resonator sensor is designed to measure glucose concentrations. The millimeter-wave-based sensor has a smaller chip size and higher sensitivity than microwave-frequency sensors. Therefore, the microfluidic channel was designed to be reusable and able to operate with a small sample volume. For alignment, a polydimethylsiloxane channel was simultaneously fabricated using a multilayer bonding film to attach the upper side of the pattern, which is concentrated in the electromagnetic field. This real-time sensor detects the glucose concentration via changes in the S11 parameter and operates at 28 GHz with an average sensitivity of 0.015669 dB/(mg/dL) within the 0–300 mg/dL range. The minimum detectable concentration and the distinguishable signal are 1 mg/dL and 0.015669 dB, respectively, from a 3.4 μL sample. The reusability and reproducibility were assessed through replicates.
Coronary Heart Disease (CHD) in Elderly Patients: Which Drug to Choose, Ticagrelor and Clopidogrel? A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Background: A new generation P2Y12 receptor inhibitor (ticagrelor) is recommended in current therapeutic guidelines to treat patients with coronary heart disease (CHD). However, it is unknown if ticagrelor is more effective than clopidogrel in elderly patients. Therefore, a systematic review was done to assess the effectiveness and safety of ticagrelor and clopidogrel in older patients with CHD to determine the appropriate antiplatelet treatment plan. Methodology: We performed a systematic review of randomized controlled trials (RCTs) to compare the effectiveness and safety of ticagrelor vs. clopidogrel in elderly patients with CHD. We selected eligible RCTs based on specified study criteria following a systematic search of PubMed and Scopus databases from January 2007 to May 2021. Primary efficacy outcomes assessed were major adverse cardiovascular events (MACEs), myocardial infarction (MI), stent thrombosis (ST), and all-cause death. The secondary outcome assessed was major bleeding events. We used RevMan 5.3 software to conduct a random-effects meta-analysis and estimated the pooled incidence and risk ratios (RRs) with 95% confidence intervals (CIs) for ticagrelor and clopidogrel. Results: Data from 6 RCTs comprising 21,827 elderly patients were extracted according to the eligibility criteria. There was no significant difference in the MACE outcome (incidence: 9.23% vs. 10.57%; RR = 0.95, 95% CI = 0.70–1.28, p = 0.72), MI (incidence: 5.40% vs. 6.23%; RR = 0.94, 95% CI= 0.69–1.27, p = 0.67), ST (incidence: 2.33% vs. 3.17%; RR = 0.61, 95% CI= 0.32–1.17, p = 0.13), and all-cause death (4.29% vs. 5.33%; RR = 0.86, 95% CI = 0.65–1.12, p = 0.25) for ticagrelor vs. clopidogrel, respectively. In addition, ticagrelor was not associated with a significant increase in the rate of major bleeding (incidence: 9.98% vs. 9.33%: RR = 1.37, 95% CI = 0.97–1.94, p = 0.07) vs. clopidogrel. Conclusions: This study did not find evidence that ticagrelor is significantly more effective or safer than clopidogrel in elderly patients with CHD.
The safety and effectiveness of mirabegron in Parkinson’s disease patients with overactive bladder: a randomized controlled trial

Abstract Purpose To assess the safety and effectiveness of mirabegron in patients with PD complaining of overactive bladder (OAB). Patients and methods From January 2017 to November 2020, we performed a prospective randomized, double-blind, placebo-controlled trial that enrolled PD patients with symptoms of OAB. The total duration of the study was 13 weeks, comprising a 1-week screening period and a 12-week treatment period. A total of 110 patients were randomized in one of two groups: treatment group (mirabegron 50 mg) or placebo group. The primary outcomes of our study were the change from baseline in OAB symptom score (OABSS) and the overactive bladder questionnaire short form (OAB-q SF) score. The secondary outcomes were the change from baseline in the mean number of micturitions/24 hours, the mean number of urgency episodes/24 hours, the mean number of urgency incontinence episodes/24 hours and the mean number of nocturia episodes/night, volume voided/micturition (ml) as recorded on a 3-day bladder diary. Safety assessments included adverse events, electrocardiogram, QT corrected for heart rate using Fridericia’s correction (QTcF) interval and blood pressure and pulse rate measurements. Results There was a significant improvement in the primary outcome and secondary outcome measures in the treatment group compared to the placebo group. Adverse events were mild and the same in the two groups. The cardiovascular safety profile was high. This study is limited by its sample size and its short follow-up period. Conclusions Mirabegron is a promising drug to control OAB symptoms in patients with PD with an excellent safety profile.
A comparison of the diagnostic accuracy of the Mini-Cog and AD8 in identifying cognitive impairment in a geriatric health screening population in China

Abstract The present study aimed to evaluate and compare the discriminative abilities of the Mini-Cog and AD8 tests in detecting cognitive impairment in a Chinese health screening population. 160 geriatric participants were enrolled at an academic medical center . The Mini-Cog, AD8, and Mini-Mental State Examination (MMSE) were used to assess the possibility of cognitive impairment. Logistic regression and receiver operator characteristic curve analyses were performed to evaluate the discriminative abilities of the tests. The prevalence of cognitive impairment was 41.25%. Logistic regression modeling showed that the Mini-Cog (odds ratio (OR) = 0.34, 95% confidence interval (CI): 0.25–0.46) and MMSE (OR = 0.58, 95%CI: 0.49–0.69) predicted cognitive impairment with 79.4% and 80.6% correct classification, respectively. While the AD8 (OR = 1.56, 95% CI: 1.32–1.85) predicted cognitive impairment with 72.5% correct classification. The areas under the receiver operating characteristic curves of the Mini-Cog, AD8 and MMSE for detecting cognitive impairment were 0.79 (95% CI: 0.72–0.85), 0.66 (95% CI: 0.58–0.73) and 0.80 (95% CI: 0.73–0.86). Both sensitivity and specificity of the Mini-Cog were superior to those of the AD8 (sensitivity 78.79% vs. 56.06%; specificity 79.79% vs. 75.53%). Cognitive screening is crucial to maintain the quality of life of older adults. Compared with the AD8, the Mini-Cog test is a more effective tool for screening cognitive impairment in older adults.
TT-10–loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction

The meager regenerative capacity of adult mammalian hearts appears to be driven by the proliferation of endogenous cardiomyocytes; thus, strategies targeting mechanisms of cardiomyocyte cell cycle regulation, such as the Hippo/Yes-associated protein (Hippo/Yap) pathway, could lead to the development of promising therapies for heart disease. The pharmacological product TT-10 increases cardiomyocyte proliferation by upregulating nuclear Yap levels. When intraperitoneal injections of TT-10 were administered to infarcted mouse hearts, the treatment promoted cardiomyocyte proliferation and was associated with declines in infarct size 1 week after administration, but cardiac function worsened at later time points. Here, we investigated whether encapsulating TT-10 into poly-lactic-co-glycolic acid nanoparticles (NPs) before administration could extend the duration of TT-10 delivery and improve the potency of TT-10 for myocardial repair. TT-10 was released from the TT-10–loaded NPs for up to 4 weeks in vitro, and intramyocardial injections of TT-10–delivered NPs stably improved cardiac function from week 1 to week 4 after administration to infarcted mouse hearts. TT-10–delivered NP treatment was also associated with significantly smaller infarcts at week 4, with increases in cardiomyocyte proliferation and nuclear Yap abundance and with declines in cardiomyocyte apoptosis. Thus, NP-mediated delivery appears to enhance both the potency and durability of TT-10 treatment for myocardial repair.
Bio-Specific Au/Fe3+ Porous Spongy Nanoclusters for Sensitive SERS Detection of Escherichia coli O157:H7

For sensitive and fast detection of Escherichia coli O157:H7, organic and inorganic hybrid Au/Fe3+ nanoclusters (NCs) were synthesized for the first time using gold nanoparticles (GNPs), bovine serum albumin, ferric chloride, phosphate-buffered saline, and antibodies. The Au/Fe3+ porous spongy NCs with large surface area showed excellent bio-specific capability for E. coli O157:H7. GNPs in Au/Fe3+ NCs functioned as signal enhancers, significantly increasing the Raman signal via the metathesis reaction product of Prussian blue and obviously improving the detection sensitivity. We combined the novel Au/Fe3+ NCs with antibody-modified magnetic nanoparticles to create a biosensor capable of sensitive detection of E. coli O157:H7, which showed a good linear response (101 to 106 cfu/mL), high detection sensitivity (2 cfu/mL), and good recovery rate (93.60–97.50%) in spiked food samples. These results make the biosensor well-suited for food safety monitoring. This strategy achieves the goal of sensitive and quantitative detection of E. coli O157:H7.
Cross-Cultural Comparison of Relationships between Empathy and Implicit Theories of Emotions (in Chinese and Russians)

The current manuscript presents the results of a cross-cultural comparison of the relationships between empathy and implicit theories of emotion in individuals from China and Russia. We hypothesized that the members of the Chinese culture would differ from the more Western Russian participants in terms of relationships between the various components of the emotional domain. Thus, we aimed to identify latent personality profiles while hypothesizing that the Chinese sample would demonstrate more prominent links between empathy and implicit theories regarding the possibility of controlling emotions. We also assumed that immediate social context could affect the results, and therefore, we compare two groups of Chinese participants—those living in China and those living in Russia, predominantly studying in Russian universities. The initial sample included Russians (N = 523), Chinese living in Russia (N = 376), and Chinese living in China (N = 423). However, following matching procedures to enable the sociodemographic comparability of samples, the final comparison was reduced to a final sample of Russians (N = 400), a sample of Chinese living in Russia (N = 363), and a sample of Chinese living in China (N = 421). We used latent class analysis and correlation analyses to test the study hypotheses. The study found that, unlike Russians, the Chinese participants demonstrated a positive correlation between incremental implicit theories of emotions and empathy. We also established significant group and gender differences. Russian women reported higher affective empathy than men, whereas Chinese women demonstrated higher affective empathy and cognitive empathy, as well as incremental implicit theories of emotion.
Biceps Tenodesis as an Attractive Alternative to Superior Labral Anterior-Posterior (SLAP) Repair for Type II SLAP Lesions in Active-Duty Military Patients Younger Than 35 Years

Background: Biceps tenodesis has been suggested as a superior surgical technique compared with isolated labral repair for superior labral anterior-posterior (SLAP) tears in patients older than 35 years. The superiority of this procedure in younger patients, however, is yet to be determined. Purpose: To compare the outcomes of arthroscopic SLAP repair with those of arthroscopic-assisted subpectoral biceps tenodesis for type II SLAP tears in active-duty military patients younger than 35 years. Study Design: Cohort study; Level of evidence, 3. Methods: Preoperative and postoperative evaluations with a minimum 5-year follow-up including the visual analog scale (VAS), the Single Assessment Numeric Evaluation (SANE), and the American Shoulder and Elbow Surgeons (ASES) shoulder score were administered, and scores were compared between 2 groups of patients younger than 35 years. One group included 25 patients who underwent SLAP repair, and the second group included 23 patients who underwent arthroscopic-assisted subpectoral biceps tenodesis. Results: The preoperative patient age (P = .3639), forward flexion (P = .8214), external rotation (P = .5134), VAS pain score (P = .4487), SANE score (P = .6614), and ASES score (P = .6519) did not vary significantly between the 2 study groups. Both groups demonstrated statistically significant increases in function as measured by the ASES and SANE and decreases in pain as measured by the VAS at a minimum of 5 years postoperatively. Also at a minimum of 5 years postoperatively, patients in the tenodesis group had lower pain (1.3 vs 2.6, respectively; P = .0358) and higher SANE (84.0 vs 63.3, respectively; P = .0001) and ASES (85.7 vs 75.4, respectively; P = .0342) scores compared with those in the repair group. Failure rate was 20.0% in the repair group versus 0.0% in the tenodesis group (P = .0234). Conclusion: Active-duty military patients younger than 35 years with type II SLAP tears had more predictable improvement in pain, better functional outcomes, and lower failure rates after biceps tenodesis compared with SLAP repair for type II SLAP tears. Overall, the results of this study indicate that arthroscopic- assisted subpectoral biceps tenodesis is superior to arthroscopic SLAP repair for the treatment of type II SLAP tears in military patients younger than 35 years.
Characterization of Extracellular Vesicles Labelled with a Lipophilic Dye Using Fluorescence Nanoparticle Tracking Analysis

Research on extracellular vesicles (EVs) has intensified over the past decade, including fluorescent membrane labeling of EVs. An optimal fluorescent method requires the size of EVs to be preserved after labeling. Lipophilic fluorescent dyes, such as CellMask™ Green (CMG), have been widely used for this purpose. Here, we investigated conditions affecting the optimum CMG labeling of EVs derived from human choriocarcinoma cells (JAr) and different biological fluids using fluorescence NTA (fl-NTA). The effect of CMG labeling on the size, concentration and zeta potential (ZP) on JAr EVs purified with different methods were measured along with biological fluid-derived EVs. With the increase of CMG dye concentration, a significant decrease in the mean size of fluorescent nanoparticles (fl-NPs) was observed. The ZP of fl-NPs originating from JAr cells with the lowest and highest dye concentrations showed a significant shift towards more and less negative ZP values, respectively. Differences in the concentration of fl-NPs were observed for JAr EVs purified using size-exclusion chromatography (SEC) alone and SEC in combination with tangential flow filtration. The proportion of CMG labeling of NPs varied across different biological sources. CMG labeling may be a reliable technique for the detection of EVs using fl-NTA.