Miracle-dz/UniFAR-FTUs huggingface dataset:
Gonadotropin-releasing hormone-like receptor 2 inversely regulates somatic proteostasis and reproduction in Caenorhabditis elegans

The quality control machinery regulates the cellular proteome to ensure proper protein homeostasis (proteostasis). In Caenorhabditis elegans, quality control networks are downregulated cell-nonautonomously by the gonadal longevity pathway or metabolic signaling at the onset of reproduction. However, how signals are mediated between the gonad and the somatic tissues is not known. Gonadotropin-releasing hormone (GnRH)-like signaling functions in the interplay between development and reproduction and have conserved roles in regulating reproduction, metabolism, and stress. We, therefore, asked whether GnRH-like signaling is involved in proteostasis collapse at the onset of reproduction. Here, we examine whether C. elegans orthologues of GnRH receptors modulate heat shock survival. We find that gnrr-2 is required for proteostasis remodeling in different somatic tissues during the transition to adulthood. We show that gnrr-2 likely functions in neurons downstream of the gonad in the gonadal-longevity pathway and modulate the somatic regulation of transcription factors HSF-1, DAF-16, and PQM-1. In parallel, gnrr-2 modulates egg-laying rates, vitellogenin production, and thus reproductive capacity. Taken together, our data suggest that gnrr-2 plays a GnRH-associated role, mediating the cross-talk between the reproduction system and the soma in the decision to commit to reproduction.
The relationship between tooth decay with stress and BMI among elementary students in Iran

Background Tooth decay is one of the most common chronic diseases among children worldwide. Stress and body mass index are also amongst the arguable risk factors which will affect people considerably. They include biological (hormones and blood sugar), socio-economic, and environmental factors and also lifestyle. In the present study, the relationship between tooth decay with stress and BMI in children was therefore investigated. Materials and methods This was a cross-sectional study of a descriptive-analytical type. A total of 350 students who were referred to the clinic of the faculty of dentistry at Qazvin University of Medical Sciences during 2021–2022 were selected through convenience sampling method to participate in the study. First, the students underwent a dental examination after being measured on their height and weight. Then, two questionnaires; namely, demographic information and Children's Stress Symptom Scale (CSSS) of Scherer and Ryan-Wenger were completed by the children's parents through self-reporting. The collected data were analyzed using SPSS software version 23 and then descriptive statistics and logistic regression were applied. Results The mean BMI of the participating students was in the normal range. The prevalence of tooth decay among participants was 76.9% (269 people). The mean and standard deviation of stress scores was 6.85 ± 4.01 out of 26, which was at a low level. Also, the mean and standard deviation of the BMI were 22.78 ± 5.28, which was within the normal range. The results of the logistic regression showed that the variables of “father's level of education,” “family's economic status,” “the experience of the toothache during the past year,” “the oral health status,” “the frequency of tooth brushing,” “flossing,” “stress,” and “BMI” were influential factors in tooth decay (P <0.05). Conclusion Students who had improper BMI, more stress, less educated fathers, families with poor economic status, the experience of toothache within the past year, poor oral health status, and those who used toothbrushes and floss to a minimal degree suffered more tooth decay. Therefore, it is necessary that we pay more attention to these students in designing and implementing educational programs to prevent tooth decay.
Intermittent theta-burst stimulation with physical exercise improves poststroke motor function: A systemic review and meta-analysis

Background Intermittent theta-burst stimulation (iTBS) is an optimized rTMS modality that could modulate the excitability of neural structures. Several studies have been conducted to investigate the efficacy of iTBS in improving the motor function of stroke patients. However, the specific role of iTBS in motor function recovery after stroke is unclear. Hence, in our study, we performed a meta-analysis to investigate the efficacy of iTBS for the motor function improvement of stroke patients. Methods MEDLINE, Embase, and Cochrane Library were searched until May 2022 for randomized controlled trials (RCTs). Results Thirteen RCTs with 334 patients were finally included in our study. The primary endpoints were the Fugl-Meyer assessment scale (FMA) and Motor Assessment Scale (MAS) change from baseline. We found that iTBS led to a significant reduction in FMA score (P = 0.002) but not in MAS score (P = 0.24) compared with the sham group. Moreover, standard 600-pulse stimulation showed a better effect on motor function improvement than the sham group (P = 0.004), however, 1200-pulse iTBS showed no effect on motor function improvement after stroke (P = 0.23). The effect of iTBS for improving motor function only exists in chronic stroke patients (P = 0.02) but not in subacute patients (P = 0.27). Conclusion This study supports that iTBS has good efficacy for improving motor function in stroke patients. Therefore, standard 600-pulse stimulation iTBS therapy is proper management and treatment for chronic stroke.
Exploring brain changes of impulse control disorders in Parkinson's disease: An ALE study

Background Previous neuroimaging studies reported inconsistent results for comparison between Parkinson's disease (PD) with impulse control disorder (PD-ICD) and without ICD (PD-no ICD). Methods A search was performed in databases (PubMed and Web of Science) to identify studies published before May 2022. An anatomic likelihood estimation (ALE) method study was made for neuroimaging studies in PD-ICD. Results The study included 20 studies (including 341 PD-ICD and 437 PD-no ICD). PD-ICD patients showed significant cortical thinning in the right inferior frontal gyrus (IFG), the right middle frontal gyrus (MFG), the left superior frontal gyrus (SFG), the right precentral gyrus (PCG) and the left cingulate gyrus (CG), compared to PD-no ICD patients. The ALE study showed reduced resting-state brain activation in the right IFG, the right PCG, the left insula and the right transverse temporal gyrus (TTG) in PD-ICD, compared to PD-no ICD patients. In addition, PD-ICD showed increased resting-state brain activation in the right caudate, the bilateral insula and the left orbital gyrus (OG), compared to PD-no ICD patients. The study indicated reduced task-related brain activation in the right caudate, the right MFG, the right lentiform nucleus (LN) and the right precuneus (PCUN) in PD-ICD, compared to PD-no ICD patients. The study showed increased task-related brain activation in the left inferior parietal lobule (IPL), the right medial frontal gyrus, the right caudate and the right PCG in PD-ICD, compared to PD-no ICD patients. Conclusions The present ALE analysis has confirmed that brain changes in frontal, temporal and basal ganglia regions are among the most frequently reported regions in PD-ICD. Deficits in these regions could play a role in diagnosis of PD-ICD.
Systematic shift in plume bending direction at Grotto Vent, Main Endeavour Field, Juan de Fuca Ridge implies changes in venting output along the Endeavour Segment

Analysis of the time-dependent behavior of the buoyant plume rising above Grotto Vent (Main Endeavour Field, Juan de Fuca Ridge) as imaged by the Cabled Observatory Vent Imaging Sonar (COVIS) between September 2010 and October of 2015 captures long term time-dependent changes in the direction of background bottom currents independent of broader oceanographic processes, indicating a systematic evolution in vent output along the Endeavour Segment of the Juan de Fuca Ridge. The behavior of buoyant plumes can be quantified by describing the volume, velocity, and orientation of the effluent relative to the seafloor, which are a convolved expression of hydrothermal flux from the seafloor and ocean bottom currents in the vicinity of the hydrothermal vent. We looked at the azimuth and inclination of the Grotto plume, which was captured in three-dimensional acoustic images by the COVIS system, at 3-h intervals during October 2010 and between October 2011 and December 2014. The distribution of plume azimuths shifts from bimodal NW and SW to SE in 2010, 2011, and 2012 to single mode NW in 2013 and 2014. Modeling of the distribution of azimuths for each year with a bimodal Gaussian indicates that the prominence of southward bottom currents decreased systematically between 2010 and 2014. Spectral analysis of the azimuthal data showed a strong semi-diurnal peak, a weak or missing diurnal peak, and some energy in the sub-inertial and weather bands. This suggests the dominant current generating processes are either not periodic (such as the entrainment fields generated by the hydrothermal plumes themselves) or are related to tidal processes. This prompted an investigation into the broader oceanographic current patterns. The surface wind patterns in buoy data at two sites in the Northeast Pacific and the incidence of sea-surface height changes related to mesoscale eddies show little systematic change over this time-period. The limited bottom current data for the Main Endeavour Field and other parts of the Endeavour Segment neither confirm nor refute our observation of a change in the bottom currents. We hypothesize that changes in venting either within the Main Endeavour Field or along the Endeavour Segment have resulted in the changes in background currents. Previous numerical simulations (Thomson et al., J. Geophys. Res., 2009, 114 (C9), C09020) showed that background bottom currents were more likely to be controlled by the local (segment-scale) venting than by outside ocean circulation or atmospheric patterns.
A method to map the interaction network of the nuclear lamina with genetically encoded photo-crosslinkers in vivo

Lamins are intermediate filaments that assemble in a meshwork at the inner nuclear periphery of metazoan cells. The nuclear periphery fulfils important functions by providing stability to the nuclear membrane, connecting the cytoskeleton with chromatin, and participating in signal transduction. Mutations in lamins interfere with these functions and cause severe, phenotypically diverse diseases collectively referred to as laminopathies. The molecular consequences of these mutations are largely unclear but likely include alterations in lamin-protein and lamin-chromatin interactions. These interactions are challenging to study biochemically mainly because the lamina is resistant to high salt and detergent concentrations and co-immunoprecipitation are susceptible to artefacts. Here, we used genetic code expansion to install photo-activated crosslinkers to capture direct lamin-protein interactions in vivo. Mapping the Ig-fold of laminC for interactions, we identified laminC-crosslink products with laminB1, LAP2, and TRIM28. We observed significant changes in the crosslink intensities between laminC mutants mimicking different phosphorylation states. Similarly, we found variations in laminC crosslink product intensities comparing asynchronous cells and cells synchronized in prophase. This method can be extended to other laminC domains or other lamins to reveal changes in their interactome as a result of mutations or cell cycle stages.
Mitochondrial metabolism determines the functional status of human sperm and correlates with semen parameters

The diagnosis of male infertility is based essentially on the patient’s medical history and a standard semen analysis. However, the latter rarely provides information on the causes of a possible infertility, emphasizing the need to extend the analysis of the sperm function. Mitochondrial function has been associated with sperm function and dysfunction, the latter primarily through the production of excessive amounts of reactive oxygen species (ROS). We hypothesized that analysis of sperm mitochondrial metabolism together with sperm ROS production could be an additional tool to improve routine semen analysis, after appropriate validations. To test our hypothesis, we performed several experiments using a non-routine method (high-resolution respirometry, HRR) to access mitochondrial function. First, we investigated whether mitochondrial function is related to human sperm motility and morphology. When mitochondrial metabolism was challenged, sperm motility decreased significantly. Additionally, morphological abnormalities in the sperm mid-piece and mitochondria were associated with global sperm defects evaluated by routine methods. Subsequently, sperm mitochondrial function was assessed by HRR. Respiratory control ratio (RCR) was determined and evaluated in the context of classical sperm analysis. In parallel, sperm hydrogen peroxide (H2O2) production and seminal plasma (SP) antioxidant capacity were measured. The percentage of sperm with progressive motility correlated positively with RCR, SP antioxidant capacity, and negatively with the concentration of extracellular H2O2 production ([H2O2]). The percentage of normal sperm morphology correlated positively with RCR and negatively with [H2O2]. Sperm morphology did not correlate with seminal plasma antioxidant capacity. Furthermore, Receiver Operating Characteristic curves were used for the first time to test the diagnostic ability of RCR, [H2O2], and SP antioxidant capacity as binary classifiers. An RCR cut off value of 3.2 was established with a sensitivity of 73% and a specificity of 61%, using reference values considered normal or abnormal in routine semen analysis. The cut off value for [H2O2] was 0.2 μM/106 sperm (sensitivity = 65%, specificity = 60%). There were no reference values for SP antioxidant capacity that distinguished between abnormal and normal sperm samples. We conclude that sperm mitochondrial function indices in combination with [H2O2] may be useful tools to complement the routine semen analysis.
PEANUT CULTIVARS SUBMITTED TO IRRIGATION LEVELS AND NITROGEN ADUBATION IN TROPICAL CLIMATE

The objective of this study was to define the best irrigation level in a sprinkler system, submitted to nitrogen doses in covering in the municipality of Tangará da Serra MT, for two cultivars of peanut (Arachis hypogaea L.). The experiment was carried out in the experimental field of the State University of Mato Grosso (UNEMAT), located geographically in latitude 14o39’S; Longitude 57o25’W, at 440 meters of altitude. The climate is classified as tropical humid megathermal (Aw), with annual average rainfall of 1,830 mm and average temperature of the air 24.4oC. The soil is classified as Dystroferric Red Latosol. The experimental design was in randomized blocks where 4 levels (30, 70, 110 and 150% of the reference evapotranspiration, ET0) were determined. In each level, 0, 30, 60, 90 and 120 Kg ha of nitrogen were applied in two peanut cultivars IAC Tatu ST and IAC Runner 886. Each plot had 24 lines per 12 m length, 4 lines for each dose of N in each level and cultivar with 0.45 m spacing between lines. The irrigation level that provided the highest productivity in the development cycle for both peanut cultivars was 110% of ET0. While the levels of 30 and 70% of the ET0 allowed greater growth in height, but smaller productivities, the 150% ET0 level showed higher yields in the husk, and lower grain yield due to the high germination rate of the grain yet in the plant. For Tatu cultivar, doses of 60 to 90 kg ha of N increase productivity, whereas for Runner cultivar nitrogen fertilization did not affect productivity.
Plasma biomarkers and their correlation in adult children of parents with Alzheimer’s disease

Family history (FH) of late-onset Alzheimer’s disease (AD) is associated with changes in several cerebrospinal fluid (CSF) biomarkers in cognitively normal individuals. However, potential changes in plasma biomarkers remain unknown. This study aimed to evaluate potential plasma biomarkers and their correlation in cognitively normal adult children (AC) and to compare this data with their AD parents and unrelated non-demented controls (NC). Participants with dementia due to AD, their AC and NC were recruited. Plasma samples were assessed for amyloid beta (Aβ)1–42, Aβ1–40, total tau (T-tau) and phosphorylated tau (P-tau). Kruskal–Wallis test was used for the comparison of this data between the three groups. Spearman rank correlation was used for evaluation of the correlations between Aβ1–40 and Aβ1–42, and T-tau and P-tau in the AD and AC groups. A total of 99 subjects completed the assessment (30 had AD; 38 were AC group; and 31 were NC). Compared with the NC group, there were significantly higher levels of Aβ1–40, P-tau, and P-tau/T-tau ratio, and lower levels of Aβ1–42 and Aβ1–42/Aβ1–40 ratio in the AD and AC groups. The correlation between the level of Aβ1–42 and Aβ1–40 and level of T-tau and P-tau was only observed in the AC but not in the AD group. AC of AD parents demonstrate some indicators of AD like their parents. Disruption to the correlation between Aβ and tau in AD may be a biomarker for the development of AD in AC, which should be examined in a longitudinal cohort.
ANALYSIS OF SIGNAL NETWORKS IN HEMATOPOIETIC STEM CELLS DETERMINING THE STEM CELL FATE

Hematopoietic stem cells (HSCs) are the unique cells having the core property of self-renewal/ proliferation and differentiation which make them capable of giving rise to all blood cell lineages. Proliferation and migration of HSCs in a regulated manner maintain healthy homeostasis by the interplay between differentiation, self-renewal/ proliferation and dormancy. Here, we identified and described functional molecular signatures eliciting different biochemical responses in molecular signaling of CD34+ HSCs in response to a cocktail of Interleukin-3 (IL-3), Fms-Like Tyrosine Kinase-3 Ligand (FLT-3), and Stem Cell Factor (SCF), using a network-based approach. The bioinformatic analysis of sum total of 22,283 gene transcripts (extracted from microarray data GSE3003) resulted into the characterization of genes (in two subgroups: up-regulated & down-regulated) eliciting stem cell responses: proliferation, differentiation and self-renewal. As in-vitro proof of concept, nine significant genes, RBM3, DHX32, PSMD9, SAR1A, SMYD3, ST6GAL1, CKAP4, CTR9 are selected for further analysis, having a key role in molecular signaling. Taken together, our results showed the identification of some novel regulatory genes through computational means and their functional importance in biological pathways.
Identiﬁcation of acute coronary syndrome via body-surface mapping and inverse electrocardiography

Acute myocardial infarction (AMI), commonly referred to as heart attacks, remain a leading cause of morbidity, mortality and healthcare expenditure in the United States, ranking as the fifth most expensive hospital diagnosis. Emergency departments in the United States receive 10 million annual visits for chest pain. Evaluation of these patients costs $10-13 billion per year [1]. Unfortunately, despite the central role of the traditional electrocardiogram (ECG) in the diagnosis of AMI, the standard ECG remains limited by both a lack of sensitivity and specificity, leading to a delay in diagnosis and consequently increased mortality, morbidity, re-admission rates, and costs [2].
D-Regulatable Gene Expression in Transgenic Mice

and brain compared to that in mice maintained on a normal diet, while 1,25-(OH)*D3 supple-mentation restored and enhanced CAT activity over control values. These data demonstrate that hOCP is sufficient to direct osteoblast-specific 1,25- (OH)2Do-sensitive gene expression in mice in addition to the unexpected regulatable expression in brain tissue. (Molecular Endocrinology 482-487, 1993)
Powering up causal generalization: A model of human conceptual bootstrapping with adaptor grammars

Human learning and generalization benefit from bootstrapping : we arrive at complex concepts by starting small and building upon past successes. In this paper, we examine a computational account of causal conceptual bootstrapping, and describe a novel experiment in which the sequence of training data results in a dramatic order effect: participants succeed in identifying a compound concept only after experiencing training data in a “helpful” order. Our computational model represents causal relations as reusable, modular programs, which can themselves be “chunked” and flexibly reused to tackle more complex tasks. Our specific approach is based in combinatory logic and adaptor grammars, building on previous theories that posit a “language of thought” for concept representation, but making the learning process more sensitive to a learner’s experiences than any partic-ular choice of conceptual primitives. Crucially, we demonstrate that a caching mechanism like that used in adaptor grammars is key to explain human-like bootstrapping patterns in causal generalization.
Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp. MC3B-10

A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its highest level (94%) at 50 °C, pH 10 and 3.5% NaCl content. It was not toxic to Artemia salina nauplii. Microbactan is, therefore, a non-toxic and non-ionic bioemulsifier of high molecular weight with affinity for a range of oily substrates. Comparative phylogenetic assessment of the 16S rDNA gene of Microbacterium sp. MC3B-10 with genes derived from other marine Microbacterium species suggested that this genus is well represented in coastal zones. The chemical nature and stability of the bioemulsifier suggest its potential application in bioremediation of marine environments and in cosmetics. Reprinted from Int. J. Mol. Sci. Cite as: Camacho-Chab, J.C. ; Guézennec, J.; Chan-Bacab, M.J.; Ríos-Leal, E. ; Sinquin, C. ; Muñiz-Salazar, R. ; del C. De la Rosa-García, S. ; Reyes-Estebanez, M. ; Ortega-Morales, B.O. Emulsifying Activity and Stability of a Non-Toxic Bioemulsifier Synthesized by Microbacterium sp. MC3B-10. Int. J. Mol. Sci. 2013, 14, 18959-18972.