Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional analysis of the cell adhesion molecules L1, CHL1 and NCAM in vivo

Das neurale Zellerkennungsmolekul L1 ist ein Mitglied der Immunglobulin-Superfamilie und der Grunder der L1 Unterfamilie. Es fuhrt wichtige Funktionen im sich entwickelnden und im adulten Nervensystem aus. Dieser Standpunkt wird bekraftigt durch die Tatsache, dass Mutationen im humanen L1 Gen eine schwere neurologische Krankheit verursachen, die CRASH genannt wird (ein Akronym fur corpus callosum hypoplasia, retardation, adducted thumbs, spastic paraplegia, and hydrocephalus). X-Chromosom-gebundener Hydrocephalus ist sicherlich das markanteste Symptom des CRASH-Syndroms. Maus-Mutanten, denen das L1 Gen fehlt, entwickeln ebenfalls erweiterte Ventrikel. Diese Studie zeigt, dass erweiterte Ventrikel in L1-defizienten Mausen weder mit einer Stenose des Aquaeductus Sylvius, noch mit ultrastrukturellen Abnormalitaten von Ependymalzellen entlang der lateralen Ventrikel und des Aquaeductes einhergehen. Dennoch wiesen einige L1-Maus-Mutanten einen schwerwiegenden Hydrocephalus auf, charakterisiert durch eine signifikante Vergroserung des Schadels und fast vollstandige Atrophie des cerebralen Cortex. Der Aquaeduct dieser stark betroffenen Tiere war vollstandig verschlossen. Da mutierte Tiere aus zwei unabhangig voneinander hergestellten L1-defizienten Maus-Linien ahnliche Phanotypen aufweisen, wird die schwere Form des Hydrocephalus als eine spezifische Folge der L1-Defizienz angesehen. Ein weiteres Mitglied der L1-Familie von Zellerkennungsmolekulen ist das "close homologue of L1" (CHL1). Dieses Protein wird von einer Vielzahl von Nervenzellen und Subpopulationen von Gliazellen in vivo expremiert und fordert Neuritenwachstum und das Uberleben von Nervenzellen in vitro. Hier wird gezeigt, dass Gliazellen die Expression von CHL1 hochregulieren konnen als Folge einer intraorbitalen Quetschung des adulten optischen Nerven. Die Funktionen eines dritten Mitgliedes der Ig-Superfamilie, das neurale Zelladhasionsmolekul (NCAM), wurden ebenfalls untersucht. Es wird in Verbindung gebracht mit Zellmigration, Myelinisierung, synaptischer Plastizitat und Verlangerung, Faszikulierung und Wegfindung von Axonen. In dieser Studie, werden NCAM-defiziente Mause verwendet, um die Funktion von NCAM wahrend der Entwicklung des corticospinalen Traktes zu untersuchen. Es konnte eine schwere Hypoplasie des corticospinalen Traktes in adulten NCAM-defizienten Mausen nachgewiesen werden. Anterogrades Tracen des Traktes von fruh postnatalen NCAM-Mutanten zeigten ausgepragte Wegfindungsfehler corticospinaler Axone. Zusammengefasst weisen die Ergebnisse dieser Arbeit auf die Bedeutung von L1 und NCAM fur die normale Entwicklung des Nervensystems hin. Sie deuten auch eine wichtige Funktion von CHL1 im ladierten zentralen Nervensystem an.
OraQuick® ADVANCE Rapid HIV-1/2 antibody test

Rapid HIV antibody tests represent a key development in the current diagnosis and management of HIV infection. The OraQuick® ADVANCE Rapid HIV-1/2 antibody test (OraSure Technologies) has received US Food and Drug Administration approval on the basis of its performance characteristics and a subsequent Clinical Laboratory Improvement Amendments waiver based on its simplicity and accuracy. The test has been approved for use on oral mucosal transudate, whole blood or plasma. Clinical evaluation of the OraQuick ADVANCE Rapid HIV-1/2 antibody test has revealed high sensitivity and specificity. The test has many important applications, extending the opportunities for voluntary counseling and testing, and as a tool for the scale-up of antiretroviral therapy in resource-limited settings.
Isolation of Anti Ageing Molecules Secreted by Human Gingival Stem Cells. Protection of Collagen and Elastic Networks

Skin aging results in an imbalance between synthesis and degradation of the extracellular matrix. Overproduction of degradative enzymes (MMPs) and oxygen free radicals during chronological and photo-induced aging leads a degradation of the network and elastic skin collagen. Our previous work demonstrated that a culture supernatant of gingival stem cells had anti-aging activities in vitro, ex vivo and in vivo in humans. However, this culture medium is very complex and currently not responding to the European cosmetics regulation. After the analysis of the culture medium we have isolated 4 molecules interesting in terms of skin anti-aging activity: TIMP1, Selenium, Folic Acid and Glycin (TSAG). After the study of the most efficiency concentration of each molecule, the composition of TSAG is tested on irradiated UVA human dermal fibroblast and human skin. TSAG preserves the elastin and collagen network and inhibits the MMP1, MMP3 and MMP9 activities. The advantage of this mimetic solution of stem cells is to be stable, easily reproducible and non-human origin. The outlook for cosmetology seems interesting.
A pre-experiment on effects of horizontal and vertical touch displays on group work in card classification tasks

In order to verify the strengths and weaknesses of horizontal and vertical interactive displays, we designed a card classification task in which the directional properties of information are considered. We especially focused on investigating the impact of the directional properties of information on user performance and exploring the optimum group size for horizontal and vertical touch displays of the same size. We developed and evaluated some objective and simple methods to measure and analyze user performance including eye contact, time of utterance, and user arrangement. A pre-experiment was conducted in which groups ranging from two to six people performed the task on both a horizontal and a vertical display. Results show that, during the card classification task, more eye contact around the horizontal display but no difference in verbal participation between the two environments. Some evidence indicates the user performance was affected by the directional properties of information. We found the difference in user arrangement of different group size between the two environments in our tasks. We expect to conduct a large-scale experiment to collect more evidence to test our hypotheses.
Studies on the development of ameloblasts. I. Fine structure.

The cellular and fine-structural changes which take place during differentiation of ameloblasts into highly secretory cells involve growth and elongation of the cytoplasm, a change in polarity, and sequential development of organelles. The undifferentiated cells of the ameloblastic layer seem to be mainly concerned with the production of material for internal use, as they are rich in free ribosomes and have only few profiles of rough-surfaced endoplasmic reticulum. First signs of differentiation are detected when the cells begin to elongate and large numbers of microtubules oriented parallel to the cell long axis appear in the distal cytoplasm. This suggests that the microtubules provide directional guidance for the elongation of the cells. The next stage of differentiation is characterized by proliferation of the rough-surfaced endoplasmic reticulum in the distal cytoplasm and the migration of the small Golgi complex, initially at the base of the cell, to the distal cytoplasm. Intracisternal accumulation of enamel matrix-like material in the rough-surfaced endoplasmic reticulum at this stage suggests that the Golgi complex is unable to process for secretion the materials synthesized in the rough-surfaced endoplasmic reticulum. However, the presence of enamel matrix-like material in the intercellular spaces may indicate an attempt to secrete matrix by a mechanism which by-passes the Golgi complex. With further differentiation the Golgi complex reaches the distal cytoplasm where it develops and becomes very extensive. The appearance of enamel matrix-like material in the cisternae of the mature Golgi complex and in large secretion granules in secretory ameloblasts suggests that secretory material is processed in a manner similar to that of other exocrine cells. As soon as enamel matrix begins to be deposited at the distal surface, ameloblasts withdraw, leaving the cytoplasmic extension (Tomes process) in the early calcifying enamel. The presence of microtubules and filaments in both the early and late developing Tomes process suggests that they play a role in the directional and localized growth of this constricted cell extension. Based on previous evidence that the Tomes process has an extensive lysosomal system and on the observation that its surface membrane possesses numerous invaginations and coated pits, it is concluded that the present findings support the concept that the Tomes process - via its lysosomal vacuolar apparatus - plays a role in the changes which occur in the enamel matrix in early amelogenesis.
Long‐term changes in hypoxia and soluble reactive phosphorus in the hypolimnion of a large temperate lake: consequences of a climate regime shift

The (Lower) Lake of Zurich provides an ideal system for studying the long‐term impact of environmental change on deep‐water hypoxia because of its sensitivity to climatic forcing, its history of eutrophication and subsequent oligotrophication, and the quality and length of its data set. Based on 39 years (1972–2010) of measured profiles of temperature, oxygen concentration and phosphorus (P) concentration, the potentially confounding effects of oligotrophication and climatic forcing on the occurrence and extent of deep‐water hypoxia in the lake were investigated. The time‐series of Nürnberg's hypoxic factor (HF) for the lake can be divided into three distinct segments: (i) a segment of consistently low HF from 1972 to the late‐1980s climate regime shift (CRS); (ii) a transitional segment between the late‐1980s CRS and approximately 2000 within which the HF was highly variable; and (iii) a segment of consistently high HF thereafter. The increase in hypoxia during the study period was not a consequence of a change in trophic status, as the lake underwent oligotrophication as a result of reduced external P loading during this time. Instead, wavelet analysis suggests that changes in the lake's mixing regime, initiated by the late‐1980s CRS, ultimately led to a delayed but abrupt decrease in the deep‐water oxygen concentration, resulting in a general expansion of the hypoxic zone in autumn. Even after detrending to remove long‐term effects, the concentration of soluble reactive P in the bottom water of the lake was highly correlated with various measures of hypoxia, providing quantitative evidence supporting the probable effect of hypoxia on internal P loading. Such climate‐induced, ecosystem‐scale changes, which may result in undesirable effects such as a decline in water quality and a reduction in coldwater fish habitats, provide further evidence for the vulnerability of large temperate lakes to predicted increases in global air temperature.
Abolition of sex-dependent effects of prenatal exposure to diethylstilbestrol on emotional behavior in estrogen receptor-&agr; knockout mice

To investigate the contribution of estrogen receptor-&agr; in the effects of prenatal exposure to diethylstilbestrol on emotionality, estrogen receptor-&agr; knockout heterozygous pregnant mice were orally given 0.1 μg/animal of diethylstilbestrol from gestational day 11 to 17. Emotional behavior of the offspring was assessed at 5 weeks in light–dark transition tests. Time spent in the light area was significantly decreased (i.e. decrease of emotionality) by diethylstilbestrol exposure in wild-type female mice, whereas in wild-type male mice this measurement tended to be increased (i.e. increase of emotionality) by diethylstilbestrol treatment. These sex-dependent effects of diethylstilbestrol were completely abolished in estrogen receptor-&agr; knockout mice. These results suggest that the sex-dependent effects of diethylstilbestrol on emotionality are mainly produced by its action on estrogen receptor-&agr;.
DDS Bridge for Embedded System

DDS(Data Distribution Service) is the messaging middleware for data centric publish/subscribe communication. However, DDS isn’t suitable for devices with limited computing resources such as smart phones or sensors because of the size of DDS. In order to overcome this limitation, we propose the DDS Bridge which is enables small devices without DDS middleware to communicate with devices with DDS. DDSB makes DDS interwork with the any IP basedcommunication protocols. Therefore, DDSB can efficiently use DDS in ubiquitous environment.
Dual circularly polarized waveguide antenna array for satellite communications in the X band

In this paper, a novel dual circular polarization waveguide antenna array with wide band and low axial ratio is presented at X-band. Planar horn array is used to attain dual circular-polarization working with orthomode transducer and rectangular side wall coupler. A demo array of 16×16 elements is designed, manufactured and tested. The experimental results show that the AR is less than 1.5dB, the VSWR is less than 1.5 in the operation frequency band.
Yeast extracts are produced by yeast cell wall degradation using endogenous or exogenous enzymes ( Breddam

Podpora B. , Świderski F. , Sadowska A. , Rakowska R. , Wasiak-Zys G. (2016): Spent brewer’s yeast extracts as a new component of functional food. Czech J. Food Sci., 34: 554–563. The use of yeast extracts as a natural and valuable additive ingredient intended for the production of functional food and dietary supplements were demonstrated. The chemical composition, amino acid analysis, determination of protein molecular weights, antioxidant properties, and sensory evaluation were carried out for two yeast extracts. It was found that the tested extracts are characterised by high essential amino acid content, exceeding the levels of reference protein developed by the FAO/WHO, and high antioxidant activity. Sensory characteristics of tested extracts may favourably influence the quality of the proposed functional foods and dietary supplements. The obtained results indicate that the tested extracts can be utilised as a source of free amino acids and peptides in the design of functional foods and dietary supplements.
Does Simian Virus 40 Cause Non-Hodgkin Lymphoma? A Review of the Laboratory and Epidemiological Evidence

Recently, several studies have reported the detection of DNA from simian virus 40 (SV40), a macaque polyomavirus, in tumor tissues obtained from non-Hodgkin lymphoma (NHL) patients. SV40 accidentally contaminated poliovirus vaccines administered to millions of individuals in 1955–1962. A link between SV40 and NHL is biologically plausible because SV40 causes hematological malignancies in laboratory rodents. However, detection of SV40 DNA in human NHL tumors has not been confirmed by other laboratories. Casting doubt on an association between SV40 and NHL, follow-up studies of recipients of SV40-contaminated poliovirus vaccines have not revealed these individuals to be at increased risk of NHL. Furthermore, 2 recent case-control studies of NHL documented only infrequent SV40 antibody reactivity among NHL cases, and the prevalence of SV40 antibodies was similar in cases and controls. This review summarizes recent laboratory and epidemiological studies bearing on the question of whether SV40 is a cause of NHL in humans. The strengths and weaknesses of these data are discussed, and a framework for considering the collected evidence is presented. Many of the considerations raised in this review apply to the evaluation of data regarding other cancers, such as mesothelioma, brain tumors, and various sarcomas, for which an etiologic link with SV40 also has been proposed.
Impaired energy‐dependent processes underlie acute lead neuropathy

The mechanisms of lead neuropathy remain unexplained. A 48‐year‐old painter presented after undertaking a 3‐week project of paint removal without the use of a protective mask. Two weeks later, he developed fasciculations, weakness, and muscle wasting. Nerve conduction studies demonstrated a motor neuropathy. A high serum lead level was identified, leading to a diagnosis of acute lead neuropathy.
[The lived experiences of stroke caregivers three months after discharge of patients from rehabilitation hospitals].

AIM Caregiving of stroke patients has a long trajectory and the experiences of caregi- vers may change over time. Few studies have described the experience of stroke caregi- vers three months after discharge of patients from rehabilitation hospitals. This study describes the experience of stroke caregivers three months after patients are discharged home from a rehabilitation hospital. METHOD phenomenological study RESULTS Seven themes emerged from analysis: deeply-changed life; re-appreciation of the relationship with patient and family members; increased workload; difficulties in getting support from the national health-care system; lack of preparation for caregiving; difficulties in coordinating caregiving, family and personal life; relief after patient's recovery. CONCLUSIONS A comprehensive understanding of stroke caregivers' experience during the three months after patient discharge can support the development of educational interventions aimed at improving the caregiver preparation for the care of patients.
Development of an Antibacterial Root Canal Filling System Containing MDPB

An antibacterial monomer 12-methacryloyloxydodecylpyridinum bromide (MDPB)-containing experimental, chemically cured primer was prepared to develop a new resin-based root canal filling system. This study investigated the antibacterial effects of the MDPB-containing primer (experimental primer [EP]) against Enterococcus faecalis and assessed the in vitro bonding and sealing abilities of the filling system, consisting of EP and a Bis-GMA-based sealer resin. Antibacterial effects of EP were evaluated by contact with planktonic or adherent bacteria for 30 or 60 sec, and the viable bacterial number was counted. The antibacterial effects against E. faecalis in dentinal tubules were also assessed, according to a root canal infection model. Bonding and sealing abilities of the experimental filling system were examined by microtensile bond strength tests and leakage tests based on fluid filtration methods. Significantly greater reduction in viable bacteria in planktonic and adherent form was obtained by short-period contact with EP compared with the control primer (without MDPB) or with the proprietary (Epiphany) primer (p < .05). Significantly greater bactericidal effects of the EP inside the dentinal tubule of root, as opposed to the control primer or Epiphany primer, were confirmed according to a root canal infection model (p < .05), and 100% killing of E. faecalis could be obtained by the application of EP after irrigation with a 5% sodium hypochlorite solution. The experimental endodontic filling system demonstrated significantly greater bond strength to root dentin than Epiphany sealer system (Epiphany primer and Epiphany Root Canal Sealant; p < .05), showing formation of resin tags and a hybridized layer. Leakage tests clarified that the experimental system provided excellent sealing. This study confirmed that the MDPB-containing experimental antibacterial primer has the ability to effectively disinfect the root canal. Additionally, the experimental root canal filling system employing this primer and the Bis-GMA-based sealer resin is useful for achieving good sealing, suggesting its possible benefit for successful endodontic treatments.
An evaluation of a system-change training model to improve emergency department response to battered women.

OBJECTIVES To evaluate a system-change model of training from the Family Violence Prevention Fund and the Pennsylvania Coalition Against Domestic Violence for improving the effectiveness of emergency department (ED) response to intimate partner violence (IPV). METHODS An experimental design with outcomes measured at baseline, 9-12, and 18-24 months post-intervention. Twelve hospitals in Pennsylvania and California with 20,000-40,000 annual ED visits were randomly selected and randomly assigned to experimental and control conditions. Emergency department teams (physician, nurse, social worker) from each experimental hospital and a local domestic violence advocate participated in a two-day didactic information and team planning intervention. RESULTS The experimental hospitals were significantly higher than the control hospitals on a staff knowledge and attitude measure (F = 5.57, p = 0.019), on all components of the "culture of the ED" system-change indicator (F = 5.72, p = 0.04), and in patient satisfaction (F = 15.43, p < 0.001) after the intervention. There was no significant difference in the identification rates of battered women (F = 0.411, p = 0.52) (although the linear comparison was in the expected direction) in the medical records of the experimental and control hospitals. CONCLUSIONS A system-change model of IPV ED training was effective in improving staff attitudes and knowledge about battered women and in protocols and staff training, as well as patient information and satisfaction. However, change in actual clinical practice was more difficult to achieve and may be influenced by institutional policy.
How NRECA’s MultiSpeak® specification supports interoperability of diverse electric grid automation systems

NRECApsilas MultiSpeakreg specification is an industry-wide standard that facilitates interoperability of diverse business and automation applications used in electric distribution utilities. Interoperable MultiSpeak-enabled applications are already in place in numerous electric utilities and permit integrated operation of previously stand-alone systems. MultiSpeak provides similar capabilities to those included in the IEC 61968 distribution extensions to the Common Information Model (CIM).
On the complexity of the bandwidth management problem for scalable coding video streaming on a public-shared network

This paper proposes a novel concept of using the sharable bandwidth of public-shared network, like FON network, to construct an efficient video delivery system. We show that the problem of constructing an architecture to serve the video streaming requests by using a minimum amount of sharable bandwidth is NP-Hard. A linear approximation algorithm is also proposed to manage the public-shared bandwidth so that all clients are served and the total amount of employed sharable bandwidth is no greater than twice of the optimal solution.
Neuroticism Mediates the Relationship Between Childhood Adversity and Adult Sleep Quality

This study investigated the relationship of childhood adversity and adult sleep quality in 327 college students (91 males), with a mean age of 18.9 years (SD = 2.1) and also examined whether neuroticism significantly mediated the observed association. Regression findings indicate that the relationship between childhood adversity and adult sleep quality is significant, and that there is a stronger association in men. Furthermore, a bootstrapping approach to testing the significance of the indirect effect (i.e., mediation) indicated that neuroticism mediated this relationship in both men and women. These data suggest that otherwise healthy young adults with a history of childhood adversity are at increased risk for sleep disturbance. Neuroticism may represent a potential target for change in future insomnia interventions, particularly in adults with a history of childhood adversity.