Miracle-dz/UniFAR-FTUs huggingface dataset:
Prediction of Physical Activity Intensity with Accelerometry in Young Children

Background: An algorithm for the classification of ambulatory and non-ambulatory activities using the ratio of unfiltered to filtered synthetic acceleration measured with a triaxial accelerometer and predictive models for physical activity intensity (METs) in adults and in elementary school children has been developed. The purpose of the present study was to derive predictive equations for METs with a similar algorithm in young children. Methods: Thirty-seven healthy Japanese children (four- to six-years old) participated in this study. The five non-ambulatory activities including low-intensity activities, and five ambulatory activities were selected. The raw accelerations using a triaxial accelerometer and energy expenditure by indirect calorimetry using the Douglas bag method during each activity were collected. Results: For non-ambulatory activities, especially light-intensity non-ambulatory activities, linear regression equations with a predetermined intercept (0.9) or quadratic equations were a better fit than the linear regression. The equations were different from those for adults and elementary school children. On the other hand, the ratios of unfiltered to filtered synthetic acceleration in non-ambulatory activities were different from those in ambulatory activities, as in adults and elementary school children. Conclusions: Our calibration model for young children could accurately predict intensity of physical activity including low-intensity non-ambulatory activities.
The Toxic Effect of Herbicidal Ionic Liquids on Biogas-Producing Microbial Community

The aim of the study was to evaluate the effect of herbicidal ionic liquids on the population changes of microorganisms used in a batch anaerobic digester. The influence of the following ionic liquids: benzalkonium (2,4-dichlorophenoxy)acetate (BA)(2,4-D), benzalkonium (4-chloro-2-methylphenoxy)acetate (BA)(MCPA), didecyldimethylammonium (2,4-dichlorophenoxy)acetate (DDA)(2,4-D), didecyldimethylammonium (4-chloro-2-methylphenoxy)acetate (DDA)(MCPA), as well as reference herbicides (4-chloro-2-methylphenoxy)acetic acid (MCPA) and (2,4-dichlorophenoxy)acetic acid (2,4-D) in the form of sodium salts on biogas production efficiency was investigated. The effective concentration (EC50) values were determined for all tested compounds. (MCPA)− was the most toxic, with an EC50 value of 38.6–41.2 mg/L. The EC50 for 2,4-D was 55.7–59.8 mg/L. The addition of the test substances resulted in changes of the population structure of the microbiota which formed the fermentation pulp. The research was based on 16S rDNA analysis with the use of the Next Generation Sequencing method and the MiSeq platform (Illumina, San Diego, CA, USA). There was a significant decrease in bacteria belonging to Firmicutes and Archaea belonging to Euryarchaeota. A significant decrease of the biodiversity of the methane fermentation microbiota was also established, which was expressed by the decrease of the operational taxonomic units (OTUs) and the value of Shannon’s entropy. In order to determine the functional potential of bacterial metapopulations based on the 16SrDNAprofile, the PICRUSt(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)tool was used, which allowed to determine the gene potency of microorganisms and their ability to biodegrade the herbicides. In the framework of the conducted analysis, no key genes related to the biodegradation of MCPA or 2,4-D were found, and the observed decrease of their content in the supernatant liquid was caused by their sorption on bacterial biomass.
Sox8 is essential for M cell maturation to accelerate IgA response at the early stage after weaning in mice

Microfold (M) cells residing in the follicle-associated epithelium (FAE) of the gut-associated lymphoid tissue are specialized for antigen uptake to initiate mucosal immune responses. The molecular machinery and biological significance of M cell differentiation, however, remain to be fully elucidated. Here, we demonstrate that Sox8, a member of the SRY-related HMG box transcription factor family, is specifically expressed by M cells in the intestinal epithelium. The expression of Sox8 requires activation of RANKL-RelB signaling. Chromatin immunoprecipitation and luciferase assays revealed that Sox8 directly binds the promoter region of Gp2 to increase Gp2 expression, which is the hallmark of functionally mature M cells. Furthermore, genetic deletion of Sox8 causes a marked decrease in the number of mature M cells, resulting in reduced antigen uptake in Peyer’s patches. Consequently, juvenile Sox8-deficient mice showed attenuated germinal center reactions and antigen-specific IgA responses. These findings indicate that Sox8 plays an essential role in the development of M cells to establish mucosal immune responses.
Serum Leptin Levels in Patients with Hepatitis B: A Meta-analysis

Background and Aims: Hepatitis B virus (HBV) infection is a widespread disease affecting people worldwide. With more and more research on the deepening of the leptin, which suggested a close relationship between serum leptin levels and HBV. In this study, we explored the correlation between serum leptin levels and HBV through meta-analysis. Methods: Potentially relevant studies between 2007 and 2017 were retrieved from PubMed, Ovid, Embase, Web of Science, Weipu Journal, Chinese Biomedical, China National Knowledge Infrastructure, and Wan Fang Data. Z-test was used to determine the significance of the pooled standardized mean difference (SMD). Results: We analyzed 16 studies, which involved 884 HBV patients and 618 healthy controls. And then pooled the SMD and calculated the 95% confidence interval (CI) by using a random-effects model from heterogeneity analysis. High serum leptin levels were observed in HBV patients (SMD: 1.16, 95%CI: 0.72-1.59, P<0.00001). Stratification analysis on the ethnicity, study design, and detection method revealed significant increases in the serum leptin levels among the Asian population of the ethnicity subgroup, case-control study of the study design subgroup, and ELISA or RIA of the detection method subgroup. Conclusion: This meta-analysis suggests a significant difference between HBV patients and healthy controls. Therefore, serum leptin levels may serve as a prognostic marker for hepatitis B disease.
WHEAT BRAN EXTRACT MIMICS STATIN LIKE ACTION IN HYPERLIPIDEMIC RABBITS: AN EXPERIMENTAL STUDY

Objective: The study was aimed to evaluate the hypolipidemic and antioxidant effect of Triticum aestivum (wheat) bran extraction cholesterol-fed rabbits. Methods: The induced hyperlipidemic rabbits were treated with 70% ethanolic extract of wheat bran at a dose of 500 mg/kg body weight/day. The experimental protocol was designed for control, hyperlipidemic, wheat bran extract, and statin-treated groups for 60 days. The estimations for serum lipid profile, lipid peroxidation (LPO), and ferric reducing ability of plasma (FRAP) were done, and histopathological study of thoracic aorta was carried out. Results: Oral administration of wheat bran extract caused significant reductions in the serum lipid profile by 94.30% (total cholesterol), 96.73% (low-density lipoprotein cholesterol), and 58.28% (triglycerides). It significantly decreased the LPO and enhanced the antioxidant capacity (FRAP) activity as compared to the hyperlipidemic rabbits. Histopathology revealed that wheat bran extract treatment prevented the accumulation of cholesterol and regressed atheromatous plaques significantly in the aorta as compared to the hyperlipidemic group, and the results were comparable to that of the standard drug statin. Conclusion: The study exhibited that wheat bran extract controls atherosclerosis just like statin and can be used as a regular dietary supplement to manage cholesterol levels and prevent cardiovascular problems.
Self-Anamnesis with a Conversational User Interface: Concept and Usability Study

Abstract Objective Self-anamnesis is a procedure in which a patient answers questions about the personal medical history without interacting directly with a doctor or medical assistant. If collected digitally, the anamnesis data can be shared among the health care team. In this article, we introduce a concept for digital anamnesis collection and assess the applicability of a conversational user interface (CUI) for realizing a mobile self-anamnesis application. Materials and Methods We implemented our concept for self-anamnesis for the concrete field of music therapy. We collected requirements with respect to the application from music therapists and by reviewing the literature. A rule-based approach was chosen for realizing the anamnesis conversation between the system and the user. The Artificial Intelligence Markup Language was exploited for encapsulating the questions and responses of the system. For studying the quality of the system and analyzing performance, humanity, effect, and accessibility of the system, we performed a usability test with 22 persons. Results The current version of the self-anamnesis application is equipped with 63 questions on the music biography of a patient that are asked subsequently to the user by means of a chatbot conversation. The usability study showed that a CUI is a practical way for collecting anamnesis data. Users felt engaged of answering the questions and liked the human characteristics of the chatbot. They suggested to extend the conversation capabilities of the chatbot so that the system can react appropriately, in particular when the user is not feeling well. Conclusions We could demonstrate the applicability of a CUI for collecting anamnesis data. In contrast to digital anamnesis questionnaires, the application of a CUI provides several benefits: the user can be encouraged to complete all queries and can ask clarifying questions in case something is unclear.
Dielectric Spectroscopy Using Dual Reflection Analysis of TDR Signals †

Time-domain reflectometry (TDR) has been a powerful tool for measuring soil dielectric properties. Initiating from apparent dielectric constant (Ka) measurement up until apparent and complex dielectric spectroscopies, the embedded information in the TDR signal can be extracted to inspire our understanding of the underlying dielectric behaviors. Multiple full waveform inversion techniques have been developed to extract complex dielectric permittivity (CDP) spectrum, but most of them involved prior knowledge of input function and tedious calibration. This rendered the field dielectric spectroscopy challenging and expensive to conduct. Dual reflection analysis (DRA) is proposed in this study to measure CDP spectrum from 10 MHz to 1 GHz. DRA is a simple, robust, model-free, and source-function free algorithm which requires minimal calibration effort. The theoretical framework of DRA is established and the necessary signal processing procedures are elaborated in this study. Eight materials with different dielectric characteristics are selected to evaluate DRA’s performance, by using both simulated and experimental signals. DRA is capable of measuring non-dispersive materials very well, whereas dispersive materials require the assistance of a long-time-window (LTW) extraction method to further extend the effective bandwidth. The DRA approach is suitable for field applications that can only record a limited amount of data points and in-situ dielectric spectroscopy.
Assessing Blood Clotting and Coagulation Factors in Mice

The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc.
Intraoperative assessment of cerebral blood flow changes in normal and pathological brain tissue using an infrared camera

Abstract This study attempted to evaluate brain surface temperature using infrared thermography in patients with brain tumours, in particular those located in the vicinity of the eloquent areas. The clinical part of the study was preceded by the experiments on rats undertaken to test time-relation of cerebrocortical microflow (LDF) and brain surface temperature changes using the same infrared camera as in the clinical study. A significant positive correlation (r = 0.88, p < 0.05) between the maximum microflow changes and maximum brain temperature changes was observed. The temperature changes occurred, however, with some delay in comparison with LDF changes. In the clinical study, temperature was recorded before, during and after removal of a tumour in fifteen patients and an abscess in one patient. In patients with tumours located close to eloquent areas, temperature distribution was also measured during the awake part of the surgical procedure. The temperature of the tumour was different from the temperature of the tissues adjacent to the resected pathology. In four patients subjected to the intraoperative awake procedure, the temperature increase in the activated motor cortex region was observed. For the first time, it was possible to identify the cortical arteries supplying the eloquent areas.
4G versus 3G-enabled telemedicine in prehospital acute stroke care

Background Time to reperfusion treatment is closely related to outcome in ischemic stroke. Prehospital stroke work-up in CT-equipped mobile stroke units is effective in reducing time to thrombolytic treatment. Current evidence predominantly comes from mobile stroke units staffed with neurologists but telemedicine-guided management may be acceptable for providing neurological expertise in ambulances. With unsatisfactory experiences in third-generation (3G)-based approaches, fourth-generation (4G) networks may provide adequate audio-visual quality but systematic comparisons of technological parameters and decision-making are lacking. Methods Trained actors presented stroke symptoms and paramedics assisted the remotely guided extended National Institutes of Health Stroke Scale (eNIHSS) assessment on the mobile stroke unit in Berlin, Germany. We compared technical parameters of 4G and 3G connections, assessed audio-visual quality of examination, and analyzed reliability of neurological assessment and treatment decisions made by the remote neurologist versus the mobile stroke unit neurologist. Results 4G and 3G connections were evaluated in 40 scenarios each. Connectivity was not available in 17% of 4G- and 15% of 3G-attempts with 6% simultaneous unavailability of both networks. The remote examiners graded audio and video quality in 4G better than in 3G with slightly shorter assessment duration in 4G (mean: 9 (SD:5) vs. mean 11 (SD:3) min, p = 0.10). Reliability of the eNIHSS sum scores was high with intraclass correlation coefficients of 0.99 (95% CI: 0.987–1.00) for 4G and 0.98 (95% CI: 0.96–0.99) for 3G. None of the remote treatment decisions differed from onsite decisions. Conclusions 4G mobile communications provided higher quality of video-examination and allowed reliable remote assessment of stroke symptoms but coverage was still incomplete in both networks.
Spine Surgery and Related Research

Introduction: The present study aimed to understand the characteristics of adjacent segment stenosis post-surgery by examining the status of adjacent segment stenosis in patients with long-term follow-up after muscle-preserving selective laminectomy (SL). Methods: We examined 43 patients who underwent muscle-preserving SL at a single academic institution and were followed up for >10 years. The C2–C7 angle, C2–C7 sagittal vertical axis, range of motion, and C7 slope were measured using an X-ray lateral view. The anterior–posterior diameter of the spinal cord (AP of SC) and anterior–posterior diameter of the dural tube (AP of dura) at adjacent segment were measured using magnetic resonance imaging T2-weighted sagittal section. Residual space for the spinal cord at the adjacent segment (SAC) was calculated as the difference between AP of SC and AP of dura. Results: Four cases had cephalad adjacent segment stenosis at the last follow-up (upper stenosis (US) group), 9 cases had caudal adjacent segment stenosis ( lower stenosis (LS) group), and 30 cases had no stenosis (none (N) group). AP of SC, AP of dura, and SAC at the upper adjacent segment were significantly lower in the US group. AP of dura and SAC at the lower adjacent segment were significantly lower in the LS group. Multivariate logistic regression analysis revealed that the small AP of dura in the upper adjacent segment and small SAC in the lower adjacent segment were risk factors for developing a new stenosis. Conclusions: Decompression should be considered beforehand in adjacent segments with small AP of SC and small AP of dura when performing cervical decompression.
Influence of Manufacturing Parameters on Mechanical Properties of Porous Materials by Selective Laser Sintering

This paper presents a study on the tensile properties of Alumide and polyamide PA2200 standard samples produced by Additive manufacturing (AM) based on selective laser sintering (SLS). Because of the orthogonal trajectories of the laser beam during exposure, different orientations of the samples may lead to different mechanical properties. In order to reveal this process issue, four orientations of the samples in building envelope were investigated. For data reliability, all the other process parameters were constant for each material and every orientation. The tensile tests highlight small differences in elastic properties of the two materials, while significant differences in strength properties and energy absorption were observed. Nevertheless, Young modulus indicates high stiffness of the Alumide comparing to PA2200 samples. The stereo microscopy reveals a brittle fracture site for Alumide and a ductile fracture with longitudinal splitting zones for PA2200. From the orientation point of view, similar properties of samples oriented at 0 and 90 degrees for all investigated mechanical properties were observed. However, tensile strength was less influenced by the sample orientations.
Analysis of Flavonoids Bioactivity for Cholestatic Liver Disease: Systematic Literature Search and Experimental Approaches

Flavonoids are naturally occurring compounds that show health benefits on the liver. However, there is little investigation about identification and evaluation of new flavonoid-containing drugs for cholestatic liver disease, one of the most common liver illnesses. We aimed to a systematic search regarding efficacy of flavonoids for treatment of cholestatic liver disease, and then evaluate naringenin (NG) as representative flavonoid in an obstructive cholestasis model. We searched for information of experimental and clinical studies in four major databases without time and language limits. Intervention was defined as any flavonoid derivate compared with other flavonoid, placebo, or without comparator. In addition, we evaluated NG on a bile duct-ligated model in order to contribute evidence of its actions. Eleven experimental reports that support the efficacy of flavonoids in cholestatic liver disease were identified. However, there was no homogeneity in efficacy endpoints evaluated and methodology. On the other hand, NG showed beneficial effects by improving specific metabolic (cholesterol and lipoproteins) and liver damage (bilirubin and alkaline phosphatase) biomarkers. The review lacks homogeneous evidence about efficacy of flavonoids in experimental settings, and is susceptible to risk for bias. NG only showed improvements in specific disease biomarkers. More investigation is still needed to determine its potential for drug development.
Occupational therapists in emergency departments: A qualitative study

Introduction Globally, occupational therapists are establishing their role in emergency care, especially in emergency departments. This practice development merits investigation due to its nascence and the challenges that face emergency department professionals. A qualitative study examined the lived experience of occupational therapists in emergency departments to contribute to knowledge and inform practice development. Method Interpretative phenomenological analysis framed the research methods. Nine occupational therapists were purposively recruited with experience of emergency departments. Individual, semi-structured interviews were audio-recorded and transcribed verbatim. Interview transcripts were analysed line-by-line and interpreted using interpretative phenomenological analysis methods. Findings Theme one: ‘On the Factory Floor’ captured the experience of working in emergency departments. They were perceived as ordered environments, but nonetheless could be unpredictable, even chaotic. Theme two: ‘A Stranger in a Strange Land’ encapsulated what it was like to enter and establish a new role in the emergency department. The emergency department brings considerable personal and professional challenges, but it can offer rewards, especially enjoyment, recognition and being valued. At the time of the study, the participants were discovering how they might acculturate. Conclusion Occupational therapists are establishing their presence in the emergency department; professional identity is forming and the practice paradigm requires further consideration.
Depression and Anxiety Prevention Based on Cognitive Behavioral Therapy for At-Risk Adolescents: A Meta-Analytic Review

Depression and anxiety disorders are among the most common mental disorders during adolescence. During this life phase, the incidence of these clinical disorders rises dramatically, and even more adolescents suffer from symptoms of depression or anxiety that are just below the clinical threshold. Both clinical and subclinical levels of depression or anxiety symptoms are related to decreased functioning in various areas, such as social and academic functioning. Prevention of depression and anxiety in adolescents is therefore imperative. We conducted a meta-analytic review of the effects of school-based and community-based prevention programs that are based on cognitive behavioral therapy with the primary goal preventing depression, anxiety, or both in high risk adolescents. Articles were obtained by searching databases and hand searching reference lists of relevant articles and reviews. The selection process yielded 32 articles in the meta-analyses. One article reported on two studies and three articles reported on both depression and anxiety. This resulted in a total of 36 studies, 23 on depression and 13 on anxiety. For depression prevention aimed at high risk adolescents, meta-analysis showed a small effect of prevention programs directly after the intervention, but no effect at 3–6 months and at 12 months follow-up. For anxiety prevention aimed at high risk adolescents, no short-term effect was found, nor at 12 months follow-up. Three to six months after the preventive intervention, symptoms of anxiety were significantly decreased. Although effects on depression and anxiety symptoms were small and temporary, current findings cautiously suggest that depression and anxiety prevention programs based on CBT might have small effects on mental health of adolescents. However, it also indicates that there is still much to be gained for prevention programs. Current findings and possibilities for future research are discussed in order to further improve the effectiveness of targeted prevention on internalizing disorders.