Miracle-dz/UniFAR-FTUs huggingface dataset:
Predictors of Engagement in Family Mediation and Outcomes for Families that Fail to Engage.

An important limitation to the effectiveness of family mediation in assisting separated parents is parents failing to engage in the mediation process. In 524 parents who presented to a telephone-based mediation service, 113 (22%) initiating parents withdrew from mediation before the other parent was invited to participate, 241 (46%) initiating parents had respondent parents who declined to participate in mediation, and 170 cases (33%) completed mediation. We tested whether socio-demographic variables, psychological distress, coparental acrimony, parenting problems, or children's behavioral difficulties predicted mediation engagement. High interparental acrimony predicted failure to engage in mediation, but none of the other variables predicted mediation engagement. We followed a sample of 131 families that did not mediate and found they showed elevated psychological distress, acrimony, parenting problems and child adjustment difficulties, which remained unchanged 6 months later. Further research is needed to explore strategies to enhance respondent parent engagement with mediation, and to address the negative outcomes for those separated families not proceeding with mediation.
Esthiomene: An unusual presentation of elephantiasis

To the Editors Genital elephantiasis is an important medical problem in the tropics as it is associated with physical disability and extreme mental anguish especially in females. Genital elephantiasis is a very rare condition and due to prevalence of sexually transmitted infections (STIs) it is even rarer. Elephantiasis and chronic genital ulceration in women is called ‘Esthiomene’ [1]. A 40­year­old married female, mason by occupation, presented with swelling of the vulva and genital ulcer for the past five years. She complained of difficulty in micturition and difficulty in walking. She also had history of painful swellings in the inguinal region, on both sides, for the past seven years. There was no history of fever, cough with expectoration or weight loss. Her menstrual cycles were regular. She had two healthy children. She gave a history of her husband’s extramarital contacts. On genital examination two large nodular swellings of size 15×7×5 cmwere present involving both the labia majora (Figure 1). Multiple nodules were present on the mons pubis. Multiple superficial and deep ulcers of varying size were present on the inner aspect of both labia majora. Multiple, firm, non­tender, matted lymph nodes involving both the horizontal and vertical groups of inguinal lymph nodes were found bilaterally. Groove sign was present. Per rectal examination was normal. Systemic examination was normal. Mantoux test was negative. X­ray chest and ultrasound abdomen were LETTER TO EDITORS OPEN ACCESS
Dissipative lag synchronization of chaotic Lur'e systems with unknown disturbances

This paper is concerned with the problem of dissipative lag synchronization (LS) for two identical Lur’e systems with unknown external disturbances. The disturbances are supposed to be generated by the exogenous systems with and without time-delay. Based on the disturbance-observer-based control method, the linear matrix inequality technique and the dissipativity theory, the disturbance observers are developed to ensure the boundedness of the disturbance error dynamics and the dissipativity of the resultant LS error system. By using the sliding-mode control (SMC) approach, an integral-type sliding surface function is constructed and a dissipative SMC law is established to render the disturbance rejection and realize LS between the master and slave systems, and an integral sliding-mode controller is designed to guarantee the reachability of the specified sliding surface. Finally, the Chua’s circuit is used as a numerical example to illustrate the effectiveness of the proposed method.
Family Developmental Tasks of Pregnant Teenage Wives from Southern Thailand

บทคดยอ การวจยครงนมวตถประสงคเพอศกษาพฒนกจครอบครวของภรรยาตงครรภวยรนครรภแรกและอำนาจการทำนายพฒนกจครอบครวของภรรยาตงครรภวยรนครรภแรกในระยะแตงงาน กลมตวอยางจำนวน 120 คน เปนหญงตงครรภวยรนครรภแรกทมาฝากครรภทหนวยฝากครรภโรงพยาบาลแหงหนงในจงหวดสงขลา ภาคใตประเทศไทย โดยตอบแบบสอบถามทผวจยพฒนาขน ประกอบดวยขอมลทวไป และพฒนกจครอบครว (ความตองการดานกายภาพและการดแลบาน ความตองการดานจตใจ ความตองการดานเศรษฐกจและสงคม และความตองการดานความสมพนธทางเพศและการวางแผนครอบครว) และการสมภาษณ ใชเวลาในการเกบขอมล 4 เดอน ผลการศกษาพบวาพฒนกจครอบครวของภรรยาตงครรภวยรนในระยะแตงงาน ดานความตองการดานจตใจมคะแนนพฒนกจสงทสด ความตองการดานความสมพนธทางเพศและการวางแผนครอบครวมคะแนนพฒนกจนอยทสด และสมพนธภาพระหวางภรรยาและสามเปนตวแปรเพยงตวเดยวทมอำนาจ การทำนายพฒนกจครอบครว ขอมลจากการวจยครงนเปนประโยชนตอการใหคำปรกษากอนสมรสแกวยรนในการเตรยมความพรอมทจะรบบทบาทการเปนภรรยาในการปฏบตพฒนกจครอบครว ความรทไดจากการศกษาครงนมประโยชนสำหรบทมสขภาพททำงานเกยวของกบวยรน เพอทจะเขาใจ พฒนกจครอบครวในดานตางๆ คำสำคญ : พฒนกจครอบครว ภรรยาตงครรภวยรน ประเทศไทย Abstract The purposes of this study were to describe family developmental tasks of first time pregnant teenage wives and predictors (demographic characteristics) of family developmental tasks of pregnant teenage wives, within the context of the marital relationship. One-hundred and twenty subjects completed a researcher developed questionnaire and were interviewed, regarding their demographic characteristics and family developmental tasks (physical and house maintenance needs; psychological needs; economic and social needs; and sexual relationship and family planning needs). Subjects were obtained from one antenatal clinic located in Songkhla province, southern Thailand. Data gathering occurred over a period of four months. The results revealed that the subjects’ family developmental tasks had the highest percentile rank within the possible range of scores was psychological needs, with the lowest being sexual relationship and family planning needs. Only one demographic characteristic, relationship between wives and husbands, was found to predict family developmental tasks. This study provides information that could prove useful in premarital counseling of teenagers regarding specific family developmental tasks. The knowledge gained from this study may be helpful to health care professionals, working with teenagers, for the purpose of understanding the various aspects of family developmental tasks. Keywords: family developmental tasks, pregnant teenage wives, Thailand
Microbiology with Diseases by Body System

Designed for pre-nursing and allied health students (and also mixed-majors courses), Microbiology with Diseases by Body System, Third Edition retains the hallmark art program and clear writing style that have made Robert Bauman's book a success. This Third Edition features compelling clinical content related to students' future healthcare careers and abundant opportunities for applied student practice. Chapter-opening Clinical Cases, Emerging Diseases boxes, and Clinical Applications boxes introduce students to real-world clinical situations. Student comprehension is ensured with end-of-chapter practice that encompasses applied, visual, and conceptual understanding. This package contains: *Microbiology with Diseases by Body System, Third Edition
CONSUMER’S PERCEPTION REGARDING DAIRY HEALTH FOODS DEVELOPED AT NATIONAL DAIRY RESEARCH INSTITUTE

The present study was conducted in purposively selected two blocks of Karnal district of Haryana. Each selected block was divided in to rural and urban area. Sixty respondents were selected from rural area and sixty respondents were selected from urban area proportionately on the basis of their income to constitute a total of 120 respondents for the study. The acceptability score of health foods (Bajra Lassi, Jaljeera Drink and Mango Drink) were evaluated by using 9 point hedonic scale. The scores for different parameters such as taste, flavour, colour and appearance, saltiness, sweetness, mouth feel, overall thirst quenching ability, overall liking were recorded. The score for most of the parameters was above 6 which indicated the acceptance of the health foods.
The transition from hospital to home in parents of pediatric solid organ transplant recipients

THE TRANSITION FROM HOSPITAL TO HOME IN PARENTS OF PEDIATRIC SOLID ORGAN TRANSPLANT RECIPIENTS Stacee M. Lerret, BS, MSN, CPNP-AC/PC Marquette University, 2010 Readiness for hospital discharge is an under-investigated topic in pediatric solid organ transplant. The immediate post-operative period and first few weeks after transplant are a critical time period where patients are at high risk for transplant-related complications. A correlation design framed by Meleis‟ Transitions Theory were used to determine; (1) the influences of discharge teaching and care coordination on parent readiness for hospital discharge among parents of children who have experienced solid organ transplantation; and (2) the relationship of parent readiness for hospital discharge with coping, adherence difficulty, utilization of healthcare resources, and family impact in the first three weeks following discharge from the hospital. Qualitative data were used to supplement quantitative findings. Thirty seven parents from three pediatric transplant centers participated. Participants completed questionnaires on the day of hospital discharge and three weeks following hospital discharge. Regression analysis for quantitative data and content analysis for qualitative data were used to identify significant relationships and themes. Results contribute to the understanding of important issues surrounding the discharge transition in a specific pediatric chronic illness population. Care coordination was associated with readiness for hospital discharge. Readiness for hospital discharge was subsequently associated with post-discharge coping difficulty, adherence difficulty with medical follow-up and family impact. Implications for nursing practice, nursing education and nursing research are identified. Identifying parents who are not ready to go home from the hospital following their child‟s solid organ transplant provides an opportunity to offer additional transitional services so parents can effectively manage their child‟s recovery and continuing care at home. Transitions theory provides a useful framework for conceptualizing and investigating the discharge transition of parents of children experiencing solid organ transplant. Results fill the current gap in knowledge and contribute to the advancement of nursing science.
Serum antibody response following genital {alpha}9 human papillomavirus infection in young men.

Background. Although the prevalence of human papillomavirus (HPV) genital infection is similarly high in males and females, seroprevalence is lower in males. This study assessed rates and determinants of seroconversion after detection of genital HPV infection in young men. Methods. We investigated HPV type-specific seroconversion in a cohort of heterosexual male university students who had an α9 HPV type (HPV-16, -31, -33, -35, -52, -58, or -67) detected in the genital tract (n = 156). HPV DNA and antibodies were detected and typed using liquid bead-based multiplex assays. We calculated seroconversion using Kaplan-Meier survival analysis. Cox proportional hazards models with generalized estimating equations were used to examine associations with seroconversion. Results. Within 24 months of detecting genital HPV infection, type-specific seroconversion ranged from 4% for HPV-52 to 36% for HPV-31. HPV-16 seroconversion at 24 months was 13% (95% confidence interval [CI], 7%-25%). Among incident HPV infections, ever cigarette smoking and infection site(s) (shaft/scrotum and glans/urine vs shaft/scrotum or glans/urine only) were positively associated with type-specific seroconversion. Conclusions. For each of the α9 HPV types, type-specific seroconversion within 24 months was observed in 36% or less of infected men. Seroconversion might be related to cigarette smoking and genital site(s) infected.
Relevance to Human Cancer of N-Nitroso Compounds, Tobacco Smoke and Mycotoxins

The aetiology and prevention of common human cancers are the subjects of reviews and previously unpublished research presented in this volume, arising from a multi-disciplinary symposium held in September 1989 on N-nitroso compounds, tobacco and mycotoxins. Although tobacco smoking and chewing have long been established as risk factors, the possible role of nitroso compounds has inspired extensive research. The 110 papers on these three classes of carcinogenic agents cover epidemiological up-dates, exposures and biological monitoring, exposure prevention and dietary anti-carcinogens, and biological mechanisms and effects, including many studies of these in the People's Republic of China. The cancer sites causally or putatively associated with these exposures (liver, respiratory and alimentary tracts) are those at highest risk in developed and developing nations; several papers clarify the role of these agents in human cancers and indicate possibilities for prevention.
Genetic manipulation of myoblasts and a novel primary myosatellite cell culture system: comparing and optimizing approaches

The genetic manipulation of skeletal muscle cells in vitro is notoriously difficult, especially when using undifferentiated muscle cell lines (myoblasts) or primary muscle stem cells (myosatellites). We therefore optimized methods of gene transfer by overexpressing green fluorescent protein (GFP) in mouse C2C12 cells and in a novel system, primary rainbow trout myosatellite cells. A common lipid‐based transfection reagent was used (Lipofectamine 2000) along with three different viral vectors: adeno‐associated virus serotype 2 (AAV2), baculovirus (BAC) and lentivirus. Maximal transfection efficiencies of 49% were obtained in C2C12 cells after optimizing cell density and reagent : DNA ratio, although the GFP signal rapidly dissipated with proliferation and was not maintained with differentiation. The transduction efficiency of AAV2 was optimized to 65% by extending incubation time and decreasing cell density, although only 30% of cells retained expression after passing. A viral comparison revealed that lentivirus was most efficient at transducing C2C12 myoblasts as 97% of cells were transduced with only 106 viral genomes (vg) compared to 54% with 108 vg AAV2 and 23% with 109 vg BAC. Lentivirus also transduced 90% of primary trout myosatellites compared to 1–10% with AAV2 and BAC. The phosphoglycerate kinase 1 (pgk) promoter was 10‐fold more active than the cytomegalovirus immediate‐early promoter in C2C12 cells and both were effective in trout myosatellites. Maximal transduction of C2C12 myotubes was achieved by differentiating myoblasts previously transduced with lentivirus and the pgk promoter. Thus, our optimized protocol proved highly effective in diverse muscle cell systems and could therefore help overcome a common technological barrier.
Cryo‐electron microscopy – a primer for the non‐microscopist

Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for studying the architecture of cells, viruses and protein assemblies at molecular resolution. Recent developments in microscope design and imaging hardware, paired with enhanced image processing and automation capabilities, are poised to further advance the effectiveness of cryo‐EM methods. These developments promise to increase the speed and extent of automation, and to improve the resolutions that may be achieved, making this technology useful to determine a wide variety of biological structures. Additionally, established modalities for structure determination, such as X‐ray crystallography and nuclear magnetic resonance spectroscopy, are being routinely integrated with cryo‐EM density maps to achieve atomic‐resolution models of complex, dynamic molecular assemblies. In this review, which is directed towards readers who are not experts in cryo‐EM methodology, we provide an overview of emerging themes in the application of this technology to investigate diverse questions in biology and medicine. We discuss the ways in which these methods are being used to study structures of macromolecular assemblies that range in size from whole cells to small proteins. Finally, we include a description of how the structural information obtained by cryo‐EM is deposited and archived in a publicly accessible database.
Enhanced replication of hepatitis B virus with frameshift in the precore region found in fulminant hepatitis patients.

BACKGROUND The genotype B of hepatitis B virus (HBV) was reported to associate with fulminant hepatitis (FH). We aimed to clarify the characteristics of HBV obtained from FH patients in an area of Japan where genotype B HBV is prevalent. METHODS Using serum samples of 16 HBV-associated FH patients, partial HBV sequences were determined. The effects of HBV mutation/insertion/deletion were evaluated using an in vitro HBV replication system. RESULTS Of the 16 HBV isolates, 31% belonged to subgenotype B1/Bj, 38% were subgenotype B2/Ba, and 31% were subgenotype C2/Ce. Notably, the single nucleotide insertion/deletion that resulted in a frameshift of the precore protein was found exclusively in 60% of B1/Bj strains. An in vitro study showed that all of the frameshift mutants had significantly higher amounts of HBV DNA than did the wild type. One of the isolates had a novel insertion of A between nucleotides 1900 and 1901, which resulted in a 3-nucleotide change within the Kozak sequence of the core protein and enhanced the core protein expression in vitro. CONCLUSIONS The frameshift insertion/deletion in the precore region enhanced HBV replication and might be associated with the development of FH by the subgenotype B1/Bj HBV.
Anterior gradient 2 and 3 – two prototype androgen‐responsive genes transcriptionally upregulated by androgens and by oestrogens in prostate cancer cells

Androgens and oestrogens have been implicated in prostatic carcinogenesis and tumour progression. Although the actions of androgens have been studied extensively, the mechanisms underlying oestrogen signalling in prostate cancer are not fully understood. In the present study, we analyzed the effect of androgens and oestrogens on the expression of anterior gradient 2 (AGR2) and anterior gradient 3 (AGR3), comprising two highly‐related genes encoding secretory proteins that are expressed in prostate cancer and one of which (AGR2) has been associated with tumour metastasis. Quantitative reverse‐transcriptase PCR and western blot analysis showed androgen induction of AGR2 and AGR3 in three androgen receptor positive cell lines, starting at concentrations of 0.1 nm. Both AGR genes were also transcriptionally activated by ≥ 5 nm oestradiol but not by isotype selective or nonselective oestrogen receptor agonists in DUCaP cells that harbour a high‐level of wild‐type androgen receptor. A functional androgen receptor but not oestrogen receptor turned out to be required for both androgen and oestrogen regulation. This pattern of androgen and oestrogen regulation was confirmed in VCaP cells and was also observed for FKBP5, a well‐characterized androgen‐regulated gene. Genome‐wide chromatin‐immunoprecipitation studies coupled with deep sequencing identified androgen receptor binding sites localized in the distal promoter and intron regions of the AGR2 and AGR3 genes, respectively. The androgen responsiveness of these enhancers was verified by luciferase reporter gene assays and site‐directed mutagenesis analysis. Androgen treatment also induced p300 and RNA Pol II recruitment to androgen receptor enhancers of AGR2 and initiated local chromatin remodelling and the formation of RNA Pol II‐containing androgen receptor transcription complexes.
Murine cerebral malaria : aspects of pathogenesis and protection

The role of T-cells in development of experimental cerebral malaria was analysed in C57B1/6J and C57Bl/10 mice infected with Plasmodium berghei K173 or Plasmodium berghei ANKA by treatment with anti-CD4 or anti-CD8 MAbs. Mice were protected against experimental cerebral malaria (ECM) when anti-CD4 or anti-CD8 MAbs were injected before or during infection. Even in mice in end stage disease i.e. with a bodytemperature below 35.5°C, treatment with anti-CD4 or anti-CD8 antibodies or the combination protected against ECM whereas chloroquine treatment was completely ineffective in inhibiting further development of the cerebral syndrome.
Epidemiologic Cohort Study of Diet and Life-Style Factors among Hawaiian-American Women with Breast Cancer in Hawaii.

Abstract : This is a cohort study of Hawaiian women who have one of the highest incidence rates of breast cancer in the world. It is designed to see if type of dietary fat, specific sources of dietary fat, and alcohol use enhance breast cancer risk, and to determine if micronutrients and dietary fiber reduce risk. The study is progressing well. It has identified 24,468 women. Questionnaires were first mailed to about 20% of the women to assure that all steps on the data collecting procedures were working properly. 2290 (44.1%) out of 5198 women completed the questionnaire. We are now mailing the questionnaire to the remaining 19,270 women. Based on the results from the first 5198 subjects, it is estimated that we will recruit over 10,500 women into the study.The surveillance of these women to identify incident cases of breast cancer is being done by the Hawaii Tumor Registry, which belongs to the National Cancer Institute's Surveillance, Epidemiology and End Results Program. After a sufficient number of case are diagnosed, we will be able to study the effects of diet, physical activity and exogenous estrogen use on breast cancer in Hawaiian women.
Evolution of variety‐specific regulatory schema for expression of osa‐miR408 in indica rice varieties under drought stress

Evolution of differential regulatory mechanisms can lead to quite distinct physiological attributes. In the present study, we have identified one such regulatory schema that regulates osa‐miR408 and responds differentially in drought‐sensitive and ‐tolerant indica rice varieties. A comparison of the drought stress response in drought‐sensitive (Pusa Basmati 1 and IR64) and drought‐tolerant (Nagina 22 and Vandana) indica rice varieties revealed that, during drought stress, levels of miR408 transcript decrease significantly in sensitive cultivars, whereas they remain elevated in the tolerant cultivars. The trend is reflected in young seedlings, as well as in flag leaf and spikelets of adult plants (heading stage). Members of the plastocyanin‐like protein family targeted by miR408 also show the inverse expression profile and thus accumulate at a lower level in tolerant cultivars during drought. Interestingly, some members of this family are implicated in maintaining the cellular redox state and spikelet fertility in Arabidopsis. An investigation of miR408 loci (including promoter) in all four cultivars did not reveal any significant sequence variation indicating an involvement of the upstream regulatory schema. Indeed, a similar variety‐specific stress response was found in the Oryza sativa squamosa promoter‐binding‐like 9 transcription factor that regulates miR408 expression. We further demonstrate that drought‐mediated induction of miR408 in Nagina 22 is regulated by [Ca2+]cyt levels. However, [Ca2+]cyt does not appear to regulate miR408 levels in Pusa Basmati 1, suggesting a variety‐specific evolution of regulatory schema in rice.