Miracle-dz/UniFAR-FTUs huggingface dataset:
Intestinal host-microbe interactions and sex

In veel onderzoeksvelden worden mannen en vrouwen op dezelfde manier behandeld, terwijl ze toch verschillen vertonen in veel ziektebeelden, waaronder darm gerelateerde ziekten zoals inflammatoire darmziekte (IBD). Onze darmbacterien (microbiota) spelen een belangrijke rol in gezondheid en ziekte en hebben nauw contact met het immuunsysteem van de darmen. De verschillen in uiting van darm gerelateerde ziekten tussen mannen en vrouwen suggereert dat zowel de microbiota samenstelling als het immuunsysteem verschilt per geslacht. Daarom hebben wij de rol van geslacht op het immuunsysteem van de darm en microbiota samenstelling onderzocht. Daarnaast hebben we onderzocht of factoren zoals genetische achtergrond, leeftijd en reproductieve status mogelijke geslachtsverschillen beinvloeden. Wij vonden geslachtsverschillen in zowel darmimmuniteit als in de microbiota samenstelling, hoewel afhankelijk van de muizenstam, leeftijd en reproductieve status. Jonge gezonde mannetjes en vrouwtjes muizen hadden een verschillend microbioom en darmimmuniteit die mogelijk bijdragen aan hun geslacht specifieke behoeften. Geslachtshormonen lijken hierin een rol te spelen, aangezien dit ook het geval is voor het perifere immuunsysteem en de microbiota en darmimmuniteit veranderden in condities met verhoogde geslachtshormonen, zoals tijdens zwangerschap. De geslachtverschillen in microbiota samenstelling verdwenen in oude muizen, terwijl de meeste geslachtverschillen in het immuunsysteem van de darm behouden bleven in oude muizen. Dit suggereert dat deze immuun verschillen zich ontwikkelen op een jonge leeftijd en permanent zijn en dat de veranderingen in het microbioom tijdens veroudering geen effect hebben op de darmimmunologie. De bevindingen uit dit proefschrift dragen mogelijk bij aan de ontwikkeling van meer geslacht specifieke behandelingen voor darm gerelateerde ziekten.
IMPACT INDICATORS IN TRANSPORT INFRASTRUCTURES: A NEW MARKET FOR ENGINEERING DESIGN RESEARCHERS

Indicators are a very important guide in decision making since they translate knowledge into easy-to-handle information units. This paper aims to draw attention to a not very explored “market” for engineering design researchers, by discussing the current state-of-the-art of impact indicators for transport infrastructures and the possibilities of more representative results by means of engineering design methodologies, such as life cycle assessment, life cycle cost, and risk analysis. The contribution of these methodologies in the definition and development of indicators has been scarce for the moment; however, the complexity of the impact of infrastructures demands a more comprehensive approach that could be provided by these methodologies.
Resistance gradient of black pod disease in cocoa and selection by leaf disk assay

To characterize the gradient of resistance to black pod disease experimentally, the leaf disk assay was applied to 217 cocoa genotypes. The parameters time interval, number of leaves evaluated per genotype and distribution of experimental treatments were evaluated. Significant differences between genotypes were found, resulting in the grouping in five homogenous groups (p < 0.01). In the assessments with the leaf disk test, five and seven days after inoculation, the F value was highest after seven days, clearly separating the resistant from susceptible genotypes. There was no difference between the clones behavior and the use of more than one box to group the genotypes (p = 0.56) and the use of different leaves of the same genotype (p = 0.08). The resistance gradient observed indicates variability enough for mapping and cocoa breeding in the population.
Mindfulness Based Stress Reduction Program on Chronic Low Back Pain : A Study Investigating the Impact on Endocrine , Physical , and Psychological Functioning

Mindfulness-based stress reduction program on chronic low-back pain: A study investigating the impact on endocrine, physical, and psychologic functioning / Ardito R. B. ; Pirro P. S. ; Re T. S. ; Bonapace I. ; Menardo V. ; Bruno E. ; Gianotti L. In: JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE. ISSN 1075-5535. 23:8(2017), pp. 615-623. Original Citation: Mindfulness-based stress reduction program on chronic low-back pain: A study investigating the impact on endocrine, physical, and psychologic functioning
Harnessing Dispute Resolution in a Metropolitan Bargaining Council of South Africa.

This article reports on a qualitative, interpretivist case study resulting from a university community engagement intervention enquiring into the low labour dispute settlement rate at a South African Local Government Bargaining Council (SALGBC) in a metropolitan district. The aim was to enable the BC to increase the dispute resolution settlement rate, by identifying and providing feasible solutions for the underlying problem of a declining annual rate. The purposive sample included representatives from management and trade unions. Data was collected through a pre-drafted focus group intervention and analysed by means of content analysis. The findings suggest that the main reason why the labour dispute settlement rate dropped below two per cent included an inability to obtain the required mandates to make decisions swiftly and effectively. This resulted in the *Corresponding author
91/689/EEC on hazardous waste from a Swedish application perspective

Classification of waste according to the European Union Directive 91/689/EEC on hazardous waste from a Swedish application perspective Rolf Sjöblom, Gustav Tham, Jan-Erik Haglund and Claes Ribbing a Tekedo AB, Spinnarvägen 10, SE-611 37 Nyköping, Sweden b Telge AB, Box 633, SE-151 27 Södertälje, Sweden c Söderenergi AB, Box 7074, SE-152 27 Södertälje, Sweden d Swedish Thermal Engineering Research Institute, SE-101 53 Stockholm, Sweden *Corresponding author. Tel. +46 155 21 04 15; (Fax +46 155 21 04 15, call first); Email address: rolf.sjoblom@tekedo.se, Tekedo AB, Spinnarvägen 10, SE-611 37 Nyköping, Sweden. About the corresponding author Dr Rolf Sjöblom manages Tekedo AB which supplies consultant services with focus on materials, chemistry and process issues. The areas of work include nuclear and conventional waste management, energy generation and district heating. He is also an associate professor at the Institute of Materials Chemistry at the University of Uppsala. The professional experience includes many years of research, development and consultant work at ABB Corporate Research, Studsvik Nuclear and AF Group, Process Division, and seven years of review of the national nuclear waste programme at the Swedish Nuclear Power Inspectorate. See www.tekedo. com for further information.
Towards construction and validation of an ends-in recombination system in Escherichia coli

Homologous recombination is the primary DNA repair pathway in bacteria and it is immensely important in repairing DNA double strand breaks. Components of the homologous recombination pathway have been well conserved throughout evolution as an essential part of cell survival. Homologous recombination plays an important role in cellular processes like DNA repair as well as exchange of genetic information through chromosomal crossover. During homologous recombination, DNA strand exchange leads to formation of a heteroduplex joint between the invading and displaced DNA strands. This hetereoduplex joint is called a Holliday Junction. Resolution of the Holliday Junction proceeds via one of two pathways. In the presence of RuvC and/or RecG, Holliday Junction resolution proceeds via a “cut and paste” pathway where the invading DNA strand replaces a region of homologous DNA on the target DNA. In the absence of RuvC and RecG, Holliday Junction resolution takes place via a “copy and paste” pathway during which DNA synthesis needs to be primed at Holliday Junction intermediates formed during strand invasion. In an effort to separate this myriad of different requirements, I have attempted to develop a novel “ends-in” recombination assay system using E. coli as a model organism. This ends-in system would allow recombinant molecule formation by DNA synthesis of approximately 200 to 2000 bp size interval between the two converging ends of an invading linear dsDNA substrate oriented just like the greek letter U, but with the arms pointing inwards. In this study, a number of linear dsDNA assay templates were constructed and analyzed. All the constructs had two “arms” of homology to the chromosome pointing inwards i.e. in the ends-in orientation. Using this ends-in system, it was demonstrated that the presence of chi (Crossover Hotspot Initiator) sites was an important requirement for ends-in recombination in wild type E. coli cells. Our studies also showed that ends-in homologous recombination did not occur if chi sites were placed at or very near to the ends of the incoming linear dsDNA molecule, suggesting that the chi site recognition is efficient only if the incoming dsDNA has chi sites internal to the ends. Moreover, it was shown that neither RuvC nor RecG were required for successful recombinant product formation using the ends-in assay. This finding reinforces previous observations that suggest the idea that Holliday Junctions can be resolved independent of both RuvC and RecG.
Assessment of the current climate and expected climate changes in the Metropolitan Region of Santiago de Chile

This report describes the methodology and results from the analysis of climate scenarios and their impact on hydrometeorological variables in the Metropolitan Region of Santiago de Chile (MRS). Using a downscaling methodology for future scenarios A2 and B1, according to IPCC, temperature, precipitation and secondary variable trends were estimated for the time-window 2045-2065. The main results predict that Santiago will be a dryer and hotter city in the near future, with a high number of days with extreme temperatures. Due to lower precipitation rates, decreasing magnitudes in the streamflow of the two main rivers, Maipo and Mapocho, are expected. The presented data provide a basis for the ClimateAdaptationSantiago (CAS) project as the aim of that project is to elaborate, evaluate and prioritize adaptation measures to the climate change in the MRS.
Genetic variation for adventitious rooting in response to low phosphorus availability: potential utility for phosphorus acquisition from stratified soils.

We hypothesized that adventitious roots may improve crop adaptation to low-phosphorus soils by enhancing topsoil foraging. In a tropical field study, phosphorus stress stimulated adventitious rooting in two phosphorus-efficient genotypes of common bean (Phaseolus vulgaris L.) but not in two phosphorus-inefficient genotypes. Although phosphorus availability had no consistent effects on the length or biomass of whole root systems, it had differential effects on adventitious, basal, and taproots within root systems in a genotype-dependent manner, resulting in increased allocation to adventitious roots in efficient genotypes. Adventitious roots had greater length per unit biomass than other root types, especially under phosphorus stress. Adventitious roots had less construction cost than basal roots, despite having similar tissue nitrogen content. Phosphorus stress reduced lateral root density, and adventitious roots had less lateral root density than basal roots. Lateral roots formed further from the root tip in adventitious roots compared with basal roots, especially under phosphorus stress. Field results were confirmed in controlled environments in solid and liquid media. Stimulation of adventitious rooting by phosphorus stress tended to be greater in wild genotypes than in cultivated genotypes. We propose that adventitious rooting is a useful adaptation to low phosphorus availability, because adventitious roots explore topsoil horizons more efficiently than other root types.
Effect of the shape of mini plate on the stability of mandibular fracture

Introduction: Both thickness, width-length and shape of the miniplate along with number and dimensions of the screws play a role for fracture stability however the researches that studied the effect of the shape are few. Aim: Aim of this work is to study the effect of the miniplate shape on fracture stability while the other factors are fixed. Materials and Method: Specially designed straight and L shaped miniplates both are equal in length, width and number of the holes, used to manage symphyseal fracture of acrylic mandible whether in single or double manners the stability of the fracture was invstigated through bending and torque test. Results: L shaped plate was more stable than straight plate on both bending and torque tests. Conclusion: Changing the shape of the plate may have a role for fracture stability while other factors are fixed.
Who Wants To Give Forever? Giving Meaning To Sustainability In Development

This paper argues strongly in favour of a re‐examination of the term ‘sustainability’ in international development research, policy and practice. It demonstrates that the term is understood pluralistically, being both environmental and economic. Within economic interpretations, this paper identifies three clear understandings of ‘sustainability’, which effect programme design and, ultimately, their impact on poverty significantly. The paper argues that the ramifications of this definition go far beyond semantics and have a significant impact on the development outcomes realised by programmes that incorporate the term. Having evaluated these conceptions, the paper argues for sustainability in development as being interventions that alter the causes of poverty so that the process through which change occurs is improved in an adaptive and permanent way, a permanent increase in adaptability. Evidence is drawn from a review of the literature and policy documentation. Copyright © 2014 John Wiley & Sons, Ltd.
CALIBRATION INTERFACE FOR THE ELECTRONIC SWIM

The overhead required to learn to swim with a severe visual impairment is much lower than learning other sports. The most popular system currently in place to assist visually impaired swimmers requires a coach to stand at either end of a pool lane and tap the swimmer on the head when they reach the location where they should begin their turn. This proves problematic since two volunteers are needed and constant attention to the swimmer is required by both volunteers to prevent collisions with the pool deck. Furthermore, there is no effective means to prevent the swimmer from veering laterally into other swimming lanes.
Temporal variability of Chlorobium phaeobacteroides antenna pigments in a meromictic karstic lake

The changes in the antenna pigment composition of Chlorobiumphaeobacteroides growing in 2 meromictic basins of Lake Banyoles have been studied during a seasonal growth period. Changes involved both the increase in carotenoid content and the enrichment of the antenna in highly alkylated bacteriochlorophyll e (BChl e) homologs. Interestingly, the homolog composition at the end of the growth period was similar to those measured in laboratory cultures incubated at low Light intensity, which are characterised by high amounts of BChl e homologs possessing larger alkyl substituents at the C-8 position of the macrocycle (i.e. isobutyl and neopentyl). Furthermore, differences in the vertical distribution of both pigments were also observed. Seasonal variation in the antenna pigment composition could be interpreted as a response to gradual changes in light regime during growth, mainly caused by self-shading and by the development of an overlying population of the purple sulphur bacterium Cl~romatium minus, which acts as a biological filter for light. The increase in both antenna pigments is discussed on the basis of recent findings on the spectral properties of BChl e and carotenoids and the role in the light adaptation processes is accordingly interpreted.
Development and validation of a system for the generation, characterization and subsequent air- liquid interface studies of aerosol particles

Exposure to nano-sized and nanostructured aerosol particles with tailored properties are likely to increase in society. Occupational exposure to various kinds of nanostructured aerosol particles such as soot and metal aggregates already exists. To understand the adverse human health effects of these particles, there is a need for systems that can Generate a stable output of aerosol particles, Characterize their exposure and dose, and determine their toxicological and biological effects in an Air-liquid interface setting (GCA). The research presented in this thesis developed and validated a GCA that combines a high output of aerosol particles, online characterization, collection capabilities for a scanning electron microscope (SEM) and transmisson electron microscopy (TEM), as well as wet samples for protein interaction studies and particle toxicological effects. The GCA is composed of an air-liquid interface cellular exposure chamber – the Nano Aerosol Chamber for In Vitro Toxicity (NACIVT) – and an electrostatic precipitator. It also includes a high output aerosol particle generator with built-in capabilities for sintering and online mass mobility characterization, a scanning mobility particle sizer, and a tapered element oscillating microbalance (TEOM) for continuous characteristics and exposure measurements. The output characteristics of two spark discharge generators and a high temperature evaporation furnace were determined, including mass mobility relation. The results show that all three of these generators produced a stable output of nanostructured metal aerosol particles in the 30-300 nm size range; when sintered, these were near spherical in shape. In terms of dose, number, mass and surface area, the output was comparable to that of previous studies for all generators. The GCA with respect to toxicological studies was validated using primary human small airway epithelial cells (SAEC) and carcinoma cell line (A549). Aerosol particles of copper (Cu), palladium (Pd) and silver (Ag) were generated and administerd in the NACIVT during 1 hour exposures. For SAEC exposed to Cu, Pd or Ag, a significant (p<0.05) effect on both viability and cytokine expression was oberserved. Also a significant (p<0.05) dose response for SAEC exposed to Cu, Pd and Ag could be determined. For the A549, Cu and Pd exposure induced a significant reduction in cellular viability. The protein interactions of the near-spherical sintered and aggregted Au nanoparticles, that were generated using high temperature evaporation furnace, were investigated in serum and human lung fluid solutions. When administered to the air-liquid interface, the spherical Au particles formed a stable film with a pinkish hue; when agitated, <100 nm aggregates of particles and proteins were formed. This film formation is suggested as a protective property of the air-liquid interface itself.
Investigation of drug delivery on anticancer drug by SWCNT with theoretical studies

The interaction of anticancer drug Sn(CH3)2(N-acetyl-L-cysteinate), that is called (N-acetyl-L-cysteinato-O,S) dimethyl tin(IV), with single walled carbon nanotube (SWCNT) is investigated by Quantum chemical ab initio calculations at HF/(LanL2DZ+STO-3G) and HF/(LanL2DZ+6-31G) levels in gas phase and solution. The solvent effect is taken into account via the self-consistent reaction field (SCRF) method. Carbon nanotubes can act as a suitable drug delivery vehicle for internalization, transportation and translocation of Sn(CH3)2(N-acetyl-L-cysteinate) within biological systems. Thermodynamical analysis indicates that the relative energies (ΔE), enthalpies (ΔH) and free Gibbs energies (ΔG) are negative for Sn(CH3)2(N-acetyl-L-cysteinate)-CNT system, but the calculated entropies (ΔS) are positive, suggesting thermodynamic favorability for covalent attachment of Sn(CH3)2(N-acetyl-L-cysteinate) into carbon nanotube. Also, the results show that with increasing dielectric constant of solvent, the stability of Sn(CH3)2(N-acetyl-L-cysteinate)-CNT complex decreases. Furthermore, anisotropic chemical shift tensor (Δσ), total atomic charge and asymmetry parameter (η) have been calculated using the gauge-including atomic orbital (GIAO) method, results being compared with CGST data. From the nuclear magnetic resonance (NMR) calculations, it can be seen that the NMR (Δσ, η) parameters at the sites of nitrogen and oxygen, as well as C-2 and C-3 nuclei are significantly influenced by intermolecular hydrogen-bonding interactions, but the quantity at the site of S-27 is influenced by nonspecific solute-solvent interaction, such as polarisability/polarity. Key words: Sn(CH3)2(N-acetyl-L-cysteinate), nuclear magnetic resonance (NMR) parameters, single walled nanotube (SWNT), solvent.
Seam position detection in pulsed gas metal arc welding

The feasibility of high productivity girth welding of pipelines has previously been demonstrated. Manual adjustment of torch height and seam tracking is not however viable at the high travel speeds used. Fortunately, through arc sensing can be used to monitor and record arc voltage and current. Methodologies developed using these signals are presented to compensate for pipe ovality in-process and to maintain torch tracking whilst weaving. The successful up-take of automated girth welding is dependent on the successful implementation of these control strategies as these important process variables have been identified as being critical for proper weld bead deposition.
Antibiotic Susceptibility of Coliform and Vibrio Species in Shellfishes from Estuary Chronically Contaminated with Polycyclic Aromatic Hydrocarbon

Polycyclic aromatic hydrocarbon (PAH) levels and antibiotic susceptibility of coliform and Vibrio species in swimming crab ( Callinectes latimanus ), mangrove oyster ( Crassostrea tulipa ), and periwinkle ( Tympanotonus fuscatus ) from Qua Iboe River and Cross River estuaries were assessed using standard procedures. Bacterial load in the shellfishes and surface water was 1.2 to 1.4 times higher than the control, but the difference was significant at p = 0.05. Total PAH level of 11.36 ± 0.3 mg kg -1 in C. latimanus , 17.57 ± 0.9 mg kg -1 in C. tulipa , and 13.88 ± 0.5 mg kg -1 in T. fuscatus compared to 7.51 ± 0.3 mg L -1 in the surface water and indicates 1.06 to 1.57 times higher PAH accumulation than in surface water and was significant ( p = 0.05). The dominant coliform and Vibrio species in the shellfishes were Serratia marcescens , Enterobacter aerogenes, Escherichia coli, Klebsiella , Citrobacter diversus , and Vibrio alginolyticus, V. estuarianus, V. fischeri, V. fluvialis and V. parahaemolyticus. A mean of 21.2% difference between two study groups indicates that shellfishes were the main source of gastrointestinal illness with 43% median resistance to commercially available antibiotics. Accumulation of PAH and abundance of an emerging multiple antibiotic resistant bacterial strains is a cause of concern and potential health risk to consumers of the shellfishes. Keywords: Shellfish, coliform, Vibrio , polycyclic aromatic hydrocarbon, antibiotic resistance