Miracle-dz/UniFAR-FTUs huggingface dataset:
Cytokine-Induced YKL-40 Expression RelB/p50 Complexes Regulate

The secreted protein, YKL-40, has been proposed as a biomarker of a variety of human diseases characterized by ongoing inﬂammation, including chronic neurologic pathologies such as multiple sclerosis and Alzheimer’s disease. However, inﬂammatory mediators and the molecular mechanism responsible for enhanced expression of YKL-40 remained elusive. Using several mouse models of inﬂammation, we now show that YKL-40 expression correlated with increased expression of both IL-1 and IL-6. Furthermore, IL-1 together with IL-6 or the IL-6 family cytokine, oncostatin M, synergistically upregulated YKL-40 expression in both primary human and mouse astrocytes in vitro. The robust cytokine-driven expression of YKL-40 in astrocytes required both STAT3 and NF- k B binding elements of the YKL-40 promoter. In addition, YKL-40 expression was enhanced by constitutively active STAT3 and inhibited by dominant-negative I k B a . Surprisingly, cytokine-driven expression of YKL-40 in astrocytes was independent of the p65 subunit of NF- k B and instead required subunits RelB and p50. Mechanistically, we show that IL-1– induced RelB/p50 complex formation was further promoted by oncostatin M and that these complexes directly bound to the YKL40 promoter. Moreover, we found that expression of RelB was strongly upregulated during inﬂammation in vivo and by IL-1 in astrocytes in vitro. We propose that IL-1 and the IL-6 family of cytokines regulate YKL-40 expression during sterile inﬂammation via both STAT3 and RelB/p50 complexes. These results suggest that IL-1 may regulate the expression of speciﬁc anti-inﬂammatory genes in nonlymphoid tissues via the canonical activation of the RelB/p50 complexes. The Journal of Immunology , 2015, 194: 000–000.
GROWTH AND PRODUCTIVITY OF GIZA 80 COTTON CULTIVAR AS AFFECTED BY FOLIAR FEEDING WITH BORON AND ZINC.

Two field experiments were carried out at Sids Agric. Res. Station, Beni-Suef Governorate, in 2007 and 2008 seasons. The experiment was conducted to evaluate the effect of foliar feeding with B and/or Zn on growth and productivity of the Egyptian cotton Giza 80 cultivar ( G. barbadense L., ) to study response of growth, fruiting and yield. Besides the control treatment, two concentrations of both boron (85 and 170 ppm) and zinc (180 and 360 ppm) and their combinations were applied twice; at the start and peak of flowering. The experimental design was randomized complete blocks with four replications. Results revealed that, spraying boron and zinc either separately or in combination generally showed some positive effects on cotton growth, fruiting and yield in comparison with untreated control in both seasons, but such as some of their treatments significantly increased only plant height and number of fruiting branches/plant in 2007 season; number of open bolls/plant, boll weight and seed cotton yield/fad. in both seasons and seed index in 2008 season and significantly decreased fruit shedding % in 2008 season. Across the two studied seasons, the combined application of 85 ppm B and either 180 or 360 ppm Zn exerted the most consistently positive effects on cotton growth and fruiting and gave the highest seed cotton yield. The higher levels of either B or Zn (170 ppm B or 360 ppm Zn) showed no more benefits than those obtained of the lower ones (85 ppm B or 180 ppm Zn), rather than it tended to reduce them in case of boron. It could be concluded from this study that yield of Giza 80 cotton cultivar might be increased by spraying 85 ppm boron + 180 ppm zinc under the environmental conditions of this study or similar of them.
RETALT: Development of an Optimal GNC Solution for Recovery of an Orbital Launch Vehicle

This paper presents the Guidance, Navigation and Control solution currently in development by DEIMOS Space for RETALT (Retro Propulsion Assisted Landing Technologies), an EU Horizon 2020 project for studying launch system re-usability technology. The general high-level GNC architecture is presented, with a more in-depth discussion on the navigation and landing phase guidance solutions. The navigation solution is based on a Considered Kalman Filter and a sensor suite that includes an INS/GNSS coupled system as baseline. Navigation simulation results are presented, which demonstrate very good performance. The guidance strategy is based on direct optimal control methods via on-board optimization, which is the only available solution able to satisfy the demanding requirements for a booster recovery mission as such. Within this methodology two approaches are identified, namely single convex optimization and successive convexification, for which a trade-off is performed. The former has been preliminarily selected for the RETALT landing guidance due to its lower computational complexity but still high fidelity. High-fidelity simulation results, however, demonstrate that the fidelity achievable with this approach is not sufficient to satisfy the mission requirements, and therefore a more complex solution based on successive convexification is required.
The Effect of 2nd-Order Shape Prediction on Tracking Non-Rigid Objects

— For the tracking of non-rigid objects, we have previously proposed a shape-based predict-and-track algorithm. The method was built upon the similarity between the predicted and actual object shapes. The object shape was predicted from the movement of feature points, which were approximated by a second-order Taylor expansion. Approximate ﬁrst-order movements, the so-called optical ﬂows, were simultaneously exploited by chamfer matching of edgelets. In this paper, the effect of second-order shape prediction is quantitatively analyzed by tracking a non-rigid skier object. The method exhibits superior shape prediction performance compared to a simple linear prediction method.
Co-creation and user innovation: The role of online 3D printing platforms Journal of Engineering and Technology Management

The aim of this article is to investigate the changes brought about by online 3D printing platforms in co-creation and user innovation. As doing so requires a thorough understanding of the level of user involvement in productive processes and a clear view of the nature of co- creative processes, this article provides a ‘prosumption’ framework and a typology of co-creation activities. Then, based on case studies of 22 online 3D printing platforms, a service- based taxonomy of these platforms is constructed. The taxonomy and typology are then matched to investigate the role played by online 3D platforms in regard to the various types of co-creation activities and, consequently, how this impacts user innovation. Elsevier This an (http://creativecommons.org/licenses/by/4.0/).
High Frequency Piezo-Composite Micromachined Ultrasound Transducer Array Technology for Biomedical Imaging

in this monograph, the authors report the current advancement in high frequency piezoelectric crystal micromachined ultrasound transducers and arrays and their biomedical applications. piezoelectric ultrasound transducers operating at high frequencies (>20 mhz) are of increasing demand in recent years for medical imaging and biological particle manipulation involved therapy. The performances of transducers greatly rely on the properties of the piezoelectric materials and transduction structures, including piezoelectric coefficient (d), electromechanical coupling coefficient (k), dielectric permittivity (e) and acoustic impedance (Z). piezo-composite structures are preferred because of their relatively high electromechanical coupling coefficient and low acoustic impedance. a number of piezo-composite techniques have been developed, namely “dice and fill”, “tape-casting”, “stack and bond”, “interdigital phase bonding”, “laser micromachining” and “micro-molding”. however, these techniques are either difficult to achieve fine features or not suitable for manufacturing of high frequency ultrasound transducers (>20 mhz). The piezo-composite micromachined ultrasound transducers (pc-mUT) technique discovered over the last 10 years or so has demonstrated high performance high frequency piezo-composite ultrasound transducers. in this monograph, piezoelectric materials used for high frequency transducers is introduced first. Next, the benefits and theory of piezo composites is presented, followed by the design criteria and fabrication methods. Biomedical applications using pc-mUT and arrays will also be reported, in comparison with other ultrasound transducer techniques. The final part of this monograph describes challenges and future perspectives of this technique for biomedical applications. ASME_Bionano Monograph Jiang_FM. indd vi Manila Typesetting Company 08/29/2017 09:58PM ASME_Bionano Monograph Jiang_FM. indd vii Manila Typesetting Company 08/29/2017 09:58PM Downloaded From: http://asmedigitalcollection.asme. org on 10/21/2018 Terms of Use: http://www.asme. org/about-asme/terms-of-use Downloaded From: http://asmedigitalcollection.asme. org on 10/21/2018 Terms of Use: http://www.asme. org/about-asme/terms-of-use
Optimization and Tracking of Scalar-Valued LQG Control Under Communication Link with Synchronized or Delayed CaSI at the Decoder

—We consider a closed-loop control problem where a fully observable stochastic linear scalar-valued plant modeled as a time-invariant Gauss-Markov process is connected to a controller via a communication link that includes an { encoder, additive Gaussian noise (AGN) channel, decoder } . We examine the impact of the communication link on the performance of the closed-loop system when the decoder is allowed to have access to some causal side information (CaSI) which is modeled an impair measurement of the state process and the performance criterion is the linear quadratic cost. We use the separation principle between the control and estimation to obtain the following results: i) lower bounds on the optimal linear quadratic cost when the CaSI model at the decoder of the communication link is accessible without delay or with ﬁxed unit delay; ii) the optimal policies that achieve these lower bounds; iii) a comparison between the newly developed bounds and the known result that does not assume CaSI at the decoder.
Fuzzy Logic Based Approach for Controlling of a Vehicle in its Longitudinal Motion

: Research of many topics in intelligent transportation system (ITS) is the essential study in automobile domain. Adaptive cruise control (ACC) is one of the advanced topics. This paper describes the longitudinal control of the vehicle by using fuzzy logic based approach. The purpose of this paper is to design Fuzzy logic based Adaptive cruise control (ACC). Based on the classical controlling techniques a fuzzy logic based Accelerator controller has been developed. The compared output of the acceleration is used by another controller to decide the speed of the vehicle. Fuzzy rules have been designed according to the input to get the desired output. This controller aims to keep a safe distance from the preceding vehicle by adapting the speed of the host vehicle. Brake setting and throttle setting are the two input variables of the vehicle. Graphical user interface LabVIEW is used in order to investigate the performance of the designed controller.
Semantic Segmentation of Remote Sensing Data using Data Mining Techniques for Geological Mapping

— Conventional means of mineral exploration requires physical access to the region of interest which is a labor-intensive process with many associated challenges. Furthermore, it results in geological maps with nominal precision and is limited by many factors. Remote sensing has emerged as a prominent tool for environment mapping and land cover and land use mapping but its applications in geological mapping are comparatively limited. However, due to the availability of non-commercial satellite data sources and cloud processing services its applications are emerging in mineral exploration and geological mapping community. This study briefly explains the process of geological mapping using different data mining algorithms. Furthermore, it explores different data mining techniques for semantic segmentation of remote sensing data specifically for geological mapping and categorizes them in three classes i.e. conventional techniques, machine learning techniques, and deep learning techniques. The paper contrast and compares these techniques factoring its feasibility for lithological mapping and narrows down to a couple of most prominent techniques in each category. The findings of this study demonstrate the potential of semantic segmentation techniques for geological mapping with a review of practical case studies. Furthermore, it also explores future avenues for research in this domain. The study is beneficial for researchers in geographic information sciences and geological sciences domains working on geological mapping, land cover/land use mapping, or environment mapping.
High activity P-gp, MRP2 and BCRP membrane vesicles prepared from transiently transfected HEK293-EBNA cells

Membrane bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high activity transporter membrane vesicles from HEK 293-EBNA cells transiently transfected using Gateway-adapted pCEP4 plasmid. Transfections were scaled up to 10-L cell cultures and vesicle preparations were optimised using ultracentrifugation with a sucrose cushion, which enabled us to produce hundreds of milligrams of membrane vesicles expressing human efflux transporter proteins P-gp/MDR1 (ABCB1), MRP2 (ABCC2) and BCRP (ABCG2). Assays were developed and optimised for analysing the ATP-dependent functionality of the transporters using probe substrates and specific inhibitors. Excellent signal to noise ratios of ATP-stimulated uptake for P-gp, MRP2 and BCRP vesicles were obtained indicating high expression of functioning transporters. The uptake kinetics of the transporters was investigated by determining K m and V max using the model substrates N-methyl-quinidine (P-gp), estradiol-17 β -glucuronide (MRP2) and estrone-3-sulfate (BCRP). The ATP-dependent transport was inhibited by the model inhibitors verapamil (P-gp), benzbromarone (MRP2) and sulfasalazine (BCRP). The vesicles are thus well suited to screen for possible substrates and inhibitors in high through put systems or used for detailed mechanistic investigations of transporter kinetics of specific substances. (pH 7.0). Uptake in MRP2 vesicles of [ 3 H]E 2 17 β G (50 μ M) was measured at 37 ° C for 8 min. Benzbromarone was used as positive control inhibitor of MRP2-mediated transport in the membrane vesicles. This equation fits inhibition data to a 2-parameter equation, where the lower data limit is 0, and the upper limit is 100. IC 50 is the concentration of the inhibitor leading to half maximal inhibition, x is the inhibitor concentration and n is the slope factor. probe substrates MRP2 BCRP and P-gp (Hooiveld vesicles from Sf9 cells. The transient transfection approach and large-scale production
Low-Dose Computed Tomography Screening for Lung Cancer

In the landmark American National Lung Cancer Screening Trial (NLST), low-dose CT (LDCT) screening produced a relative mortality reduction of 20%. These results have not been replicated in any of the European studies, although these are of limited statisti- cal power. Besides doubt about the general applicability of the NLST findings, if LDCT screening is to be successfully implemented, a number of developments are still required, including better characterisation of entry criteria and refinement of screening and nod ule management protocols. The high incidence of false-positive findings increases costs and morbidity. Even when histologically malignant tumours are identified, frequently these would not have manifested as disease, i.e. they are “overdiagnosed”. These patients are liable to receive unnecessary treatment. LDCT screening is relatively expensive in comparison with other cancer screening modalities. Whilst cost-effectiveness can be improved by integration with smoking cessation programmes, how this would be done in practice remains unclear. Furthermore, individuals at high-risk of lung cancer are vir- tually by definition risk prone, raising concerns about how attractive participation in a screening programme would be, especially given the very small reported absolute risk reduction in the NLST.
Performance Optimization and Verification of a New Type of Solar Panel for Microsatellites

In this paper, a new method of replacing the conventional honeycomb aluminum panel with 3D metal printing on the microsatellite is presented. The multiobjective optimization method is used to optimize the temperature difference, compression strength, shear strength, and weight of the new type of solar panel structure. The relationships between the structural parameters and optimization targets are established, and the influence of five factors on thermal and structural properties is analyzed. Finally, a group of better structural parameters of the panel is obtained. The relative deviations between simulation analysis and model are 27.45%, 6.12%, 1.365%, and 3.27%, respectively. The optimization results show that the regression model can be used to predict thermal and structural properties of the panel, and the establishment of the model is effective. The analysis results show that the performances can be improved by 91.62%, 46.94%, 17.91%, and 10.28%, respectively. The optimized results are used for 3D metal printing, and the new type of solar panel is obtained. It is proved that the method can effectively improve the thermal and structural properties of the panel and can effectively shorten the development and manufacture cycle of the panel and also reduce the cost. It has high engineering application value.
Original Contribution Nine-Year Changes in Cardiovascular Disease Risk Factors with Weight Maintenance in the Atherosclerosis Risk in Communities Cohort

Few studies have focused on the impact of weight maintenance on cardiovascular disease risk factors or addressed whether changes differ by baseline weight status and medication usage. The authors examined these issues using 9 years of follow-up data on 3,235 men and women from the Atherosclerosis Risk in Communities (ARIC) Study who were aged 45–64 years at baseline (1987–1989). In participants not using medications, glucose (3.0 mg/dl, 95% confidence interval (CI): 2.4, 3.5) and triglycerides (10.1 mg/dl, 95% CI: 8.3, 11.9) increased, while total cholesterol ( 9.6 mg/dl, 95%CI: 10.6, 8.6), low density lipoprotein cholesterol ( 9.9 mg/dl, 95%CI: 10.9, 9.0), and high density lipoprotein cholesterol ( 1.7 mg/dl, 95% CI: 2.1, 1.3) decreased. Systolic blood pressure (7.9 mmHg, 95% CI: 7.3, 8.4) increased, but diastolic blood pressure ( 1.1 mmHg, 95% CI: 1.4, 0.7) declined. Normal weight (body mass index: 18.5–<25.0 kg/m) participants had smaller increases in glucose compared with obese (body mass index: 30.0 kg/m) participants. In contrast, the authors found less favorable changes in total, low density lipoprotein, and high density lipoprotein cholesterol, triglycerides, and diastolic blood pressure among normal weight compared with obese participants who maintained their weight. These patterns were similar across weight status groups regardless of medication usage.
CMAR_A_331222 8887..8900

*These authors contributed equally to this work Purpose: Lung cancer is one of the most commonly diagnosed cancer as well as the leading cause of cancer-related mortality worldwide, among which lung adenocarcinoma (LUAD) is the most frequent form of lung cancer. Previous studies have shown that 15hydroxyprostaglandin dehydrogenase (15-PGDH) catalyzes the oxidation of prostaglandins to reduce their biological activities and behaves as a tumor suppressor in various cancers. Thus, we aimed to systematically examine the effects of 15-PGDH overexpression on cellular processes in lung adenocarcinoma cells. Methods: The stable 15-PGDH-overexpressing A549 cell line was constructed using lentivirus particles. CCK-8 assay was used to determine the cell proliferation rate and sensitivity to cisplatin. Tandem mass tag (TMT)-based quantitative proteomic analysis was used to identify differentially expressed proteins between control and 15-PGDH-overexpression cells. The cell cycle was determined by a flow cytometer. The expression levels of mesenchymal and epithelial markers were measured using Western blotting. Wound healing and transwell assays were used to detect the cell migration and cell invasion ability, respectively. Results: Analysis of datasets in The Cancer Genome Atlas revealed that the PGDH gene expression level in the lung adenocarcinoma tissues was significantly lower than that in the pericarcinous tissues. 15-PGDH overexpression in A549 cells reduced cell proliferation rate. Quantitative proteomics revealed that 15-PGDH overexpression inhibited PI3K/AKT/mTOR signaling pathway, which is a signaling pathway driving tumor cell growth and epithelialmesenchymal transition (EMT) process. In addition, both cell cycle and DNA repair-related proteins were down-regulated in 15-PGDH overexpressed cells. 15-PGDH overexpression induced G1/S cell cycle arrest and increased susceptibility to DNA damaging reagent cisplatin. Importantly, overexpression of 15-PGDH inhibited EMT process with the downregulation of β-catenin and Snail-1 as well as upregulation of E-cadherin and ZO-1. Conclusion: 15-PGDH is a tumor suppressor in lung cancer and may serve as a potential therapeutic target to prevent lung adenocarcinoma.
A Small Heat Shock Protein Cooperates with Heat Shock Protein 70 Systems to Reactivate a Heat-Denatured Protein 1

Small heat shock proteins (sHsps) are a diverse group of heat-induced proteins that are conserved in prokaryotes and eukaryotes and are especially abundant in plants. Recent in vitro data indicate that sHsps act as molecular chaperones to prevent thermal aggregation of proteins by binding non-native intermediates, which can then be refolded in an ATP-dependent fashion by other chaperones. We used heat-denatured firefly luciferase (Luc) bound to pea (Pisum sativum) Hsp18.1 as a model to define the minimum chaperone system required for refolding of a sHsp-bound substrate. Heat-denatured Luc bound to Hsp18.1 was effectively refolded either with Hsc/Hsp70 from diverse eukaryotes plus the DnaJ homologs Hdj1 and Ydj1 (maximum 5 97% Luc reactivation with k ob 5 1.0 3 10 2 2 /min), or with prokaryotic EscherichiacoliDnaK plus DnaJ and GrpE (100% Luc reactivation, k ob 5 11.3 3 10 2 2 /min). Further-more, we show that Hsp18.1 is more effective in preventing Luc thermal aggregation than the Hsc70 or DnaK systems, and that Hsp18.1 enhances the yields of refolded Luc even when other chaperones are present during heat inactivation. These findings integrate the aggregation-preventive activity of sHsps with the protein-folding activity of the Hsp70 system and define an in vitro system for further investigation of the mechanism of sHsp action.
Hydroxychloroquine E cacy for the Treatment of Patients with COVID-19: A Systematic Review and Meta-Analysis

Nayara Izabel Viana University of Sao Paulo Ruan Pimenta University of Sao Paulo Guilherme Lopes Gonçalves University of Sao Paulo Thaynara Faria Gomes Faculdade Atenas Vanessa Ribeiro Guimarães University of Sao Paulo Victor Srougi University of Sao Paulo, Moriah Hospital Miguel Srougi University of Sao Paulo, Moriah Hospital Katia R. Leite University of Sao Paulo Sabrina T. Reis (  sasareis@gmail.com ) University of Sao Paulo