Miracle-dz/UniFAR-FTUs huggingface dataset:
Physical therapy for facial nerve paralysis (Bell's palsy): An updated and extended systematic review of the evidence for facial exercise therapy

Objective To conduct a systematic review of the effectiveness of facial exercise therapy for facial palsy patients, updating an earlier broader Cochrane review; and to provide evidence to inform the development of telerehabilitation for these patients. Data Sources MEDLINE, EMBASE, CINAHL, Cochrane Library, PEDro and AMED for relevant studies published between 01 January 2011 and 30 September 2020. Methods Predetermined inclusion/exclusion criteria were utilised to shortlist abstracts. Two reviewers independently appraised articles, systematically extracted data and assessed the quality of individual studies and reviews (using GRADE and AMSTAR-2, respectively). Thematic analysis used for evidence synthesis; no quantitative meta-analysis conducted. The review was registered with PROSPERO (CRD42017073067). Results Seven new randomised controlled trials, nine observational studies, and three quasi-experimental or pilot studies were identified (n = 854 participants). 75% utilised validated measures to record changes in facial function and/or patient-rated outcomes. High-quality trials (4/7) all reported positive impacts; as did observational studies rated as high/moderate quality (3/9). The benefit of therapy at different time points post-onset and for cases of varying clinical severity is discussed. Differences in study design prevented data pooling to strengthen estimates of therapy effects. Six new review articles identified were all rated critically low quality. Conclusion The findings of this targeted review reinforce those of the earlier more general Cochrane review. New research studies strengthen previous conclusions about the benefits of facial exercise therapy early in recovery and add to evidence of the value in chronic cases. Further standardisation of study design/outcome measures and evaluation of cost-effectiveness are recommended.
Nonisothermal nucleation in the gas phase is driven by cool subcritical clusters

Significance In a supersaturated vapor, the phase transition is initiated by the formation of clusters. While classical nucleation theory assumes clusters to be in thermal equilibrium with the surroundings, the inevitable release of latent heat and its limited dissipation during cluster formation drive the system out of thermal equilibrium. Using atomistic simulations considering both nonequilibrium and equilibrium dynamics, we present a detailed analysis of homogeneous gas-phase nucleation and cluster growth processes. Our findings show that despite the substantial amount of excess energy carried by the entire cluster population, those clusters relevant for nucleation evolve from cold to hot while the critical cluster is in thermal equilibrium with the surroundings, a mechanism proposed but also disputed in literature.
Regulatory T Cell Proportion and Phenotype Are Altered in Women Using Oral Contraception

Abstract Regulatory T (Treg) cells are a specialized CD4+ T cell subpopulation that are essential for immune homeostasis, immune tolerance, and protection against autoimmunity. There is evidence that sex-steroid hormones estrogen and progesterone modulate Treg cell abundance and phenotype in women. Since natural oscillations in these hormones are modified by hormonal contraceptives, we examined whether oral contraception (OC) use impacts Treg cells and related T cell populations. T cells were analyzed by multiparameter flow cytometry in peripheral blood collected across the menstrual cycle from healthy women either using OC or without hormonal contraception and from age-matched men. Compared to naturally cycling women, women using OC had fewer Treg cells and an altered Treg cell phenotype. Notably, Treg cells exhibiting a strongly suppressive phenotype, defined by high FOXP3, CD25, Helios, HLADR, CTLA4, and Ki67, comprised a lower proportion of total Treg cells, particularly in the early- and mid-cycle phases. The changes were moderate compared to more substantial differences in Treg cells between women and men, wherein women had fewer Treg cells—especially of the effector memory Treg cell subset—associated with more T helper type 1 (Th1) cells and CD8+ T cells and lower Treg:Th1 cell and Treg:CD8+ T cell ratios than men. These findings imply that OC can modulate the number and phenotype of peripheral blood Treg cells and raise the possibility that Treg cells contribute to the physiological changes and altered disease susceptibility linked with OC use.
In vitro characterization of iridoid and phenylethanoid glycosides from Cistanche phelypaea for nutraceutical and pharmacological applications

“Desert hyacinths” are a remarkable group of parasitic plants belonging to genus Cistanche, including more than 20 accepted species typically occurring in deserts or coastal dunes parasitizing roots of shrubs. Several Cistanche species have long been a source of traditional herbal medicine or food, being C. deserticola and C. tubulosa the most used in China. This manuscript reports the isolation and identification of some phenylethanoid and iridoid glycosides, obtained from the hydroalcoholic extract of C. phelypaea collected in Spain. The present study aims to characterize the antioxidant activity of C. phelypaea metabolites in the light of their application in nutraceutical and cosmeceutical industries and the effect of acetoside, the most abundant metabolite in C. phelypaea extract, on human keratinocyte and pluripotent stem cell proliferation and differentiation. Our study demonstrated that acetoside, besides its strong antioxidant potential, can preserve the proliferative potential of human basal keratinocytes and the stemness of mesenchymal progenitors necessary for tissue morphogenesis and renewal. Therefore, acetoside can be of practical relevance for the clinical application of human stem cell cultures in tissue engineering and regenerative medicine.
Process- and product-related impurities in the ChAdOx1 nCov-19 vaccine

ChAdOx1 nCov-19 and Ad26.COV2. S are approved vaccines inducing protective immunity against SARS-CoV-2 infection in humans by expressing the Spike protein of SARS-CoV-2. We analyzed protein content and protein composition of ChAdOx1 nCov-19 and Ad26.COV2. S by biochemical methods and by mass spectrometry. Four out of four tested lots of ChAdOx1 nCoV-19 contained significantly higher than expected levels of host cell proteins (HCPs) and of free viral proteins. The most abundant contaminating HCPs belonged to the heat-shock protein and cytoskeletal protein families. The HCP content exceeded the 400 ng specification limit per vaccine dose, as set by the European Medicines Agency (EMA) for this vaccine, by at least 25-fold and the manufacturer’s batch-release data in some of the lots by several hundred-fold. In contrast, three tested lots of the Ad26.COV2. S vaccine contained only very low amounts of HCPs. As shown for Ad26.COV2. S production of clinical grade adenovirus vaccines of high purity is feasible at an industrial scale. Correspondingly, purification procedures of the ChAdOx1 nCov-19 vaccine should be modified to remove protein impurities as good as possible. Our data also indicate that standard quality assays, as they are used in the manufacturing of proteins, have to be adapted for vectored vaccines.
Comparison of tissue tropism and host response to enteric and respiratory enteroviruses

Enteroviruses (EVs) are among the most prevalent viruses worldwide. They are characterized by a high genetic and phenotypic diversity, being able to cause a plethora of symptoms. EV-D68, a respiratory EV, and EV-D94, an enteric EV, represent an interesting paradigm of EV tropism heterogeneity. They belong to the same species, but display distinct phenotypic characteristics and in vivo tropism. Here, we used these two viruses as well as relevant 3D respiratory, intestinal and neural tissue culture models, to highlight key distinctive features of enteric and respiratory EVs. We emphasize the critical role of temperature in restricting EV-D68 tissue tropism. Using transcriptomic analysis, we underscore fundamental differences between intestinal and respiratory tissues, both in the steady-state and in response to infection. Intestinal tissues present higher cell proliferation rate and are more immunotolerant than respiratory tissues. Importantly, we highlight the different strategies applied by EV-D94 and EV-D68 towards the host antiviral response of intestinal and respiratory tissues. EV-D68 strongly activates antiviral pathways while EV-D94, on the contrary, barely induces any host defense mechanisms. In summary, our study provides an insightful characterization of the differential pathogenesis of EV-D68 and EV-D94 and the interplay with their main target tissues.
Violence against sex workers: Correlates and Changes under ‘End-Demand’ legislation in Canada: A five city study

ABSTRACT Canada is among several countries to have implemented ‘end-demand’ criminalization frameworks for sex work. Drawing on interviews with sex workers (n = 200) in five cities, we employed multivariate logistic regression to identify associations with workplace violence. We also analysed descriptive data on trafficking and on workplace violence under end-demand legislation. In the past 12 months, being unable to call 911 in a safety emergency at work for fear of police detection (Adjusted Odd Ratio AOR: 4.307, 95% Confidence Interval CI: 1.697 –10.927), being unable to screen clients due to fear of police detection (AOR: 2.175, 95% CI: 1.074 –4.405), having experienced anti-sex work housing policy/eviction (AOR: 2.031, 95% CI: 0.897–4.598), and being Indigenous (Adjusted Odd Ratio (AOR): 2.167, 95% Confidence Interval (CI): 1.060–4.428) were all independently associated with workplace violence in the past 12 months. Of those who worked prior to the law change (n = 167), a majority of respondents (80.24% (134)) reported that violence in the workplace had increased or stayed the same compared to the previous criminalization model and 87.43% (n = 146) reported it was harder or the same to get help in an emergency.
Silencing lncRNA Snhg6 mitigates bleomycin‐induced pulmonary fibrosis in mice via miR‐26a‐5p/TGF‐β1‐smads axis

Idiopathic pulmonary fibrosis (IPF) is an interstitial pulmonary disease with slow onset and high mortality. Epithelial–mesenchymal transition (EMT) is a significant condition for tissue fibrosis, and lncRNA‐Snhg6 (small nucleolar RNA host gene 6) is related to EMT in some cancer cells, but its role in pulmonary fibrosis remains obscure. Here, we found that TGF‐β1 and Snhg6 were up‐regulated in lung tissues of BLM‐induced lung fibrosis mouse, and Snhg6 expression was significantly increased in primary lung fibroblasts after BLM treatment. Snhg6 knockdown notably alleviated the pulmonary dysfunction, and the increase of fibrosis area and collagen deposition induced by BLM. MiR‐26a‐5p was downregulated in BLM‐induced fibrotic lung tissues, and it was negatively regulated by Snhg6. Silencing Snhg6 markedly alleviated the TGF‐β1‐induced increase in fibrotic marker expression, cell proliferation, migration and differentiation, as well as the nuclear transport of p‐Smad2/3 by modulating miR‐26a‐5p expression in mouse lung fibroblasts. Moreover, overexpressing Snhg6‐induced collagen accumulation and fibroblast activation in fibroblasts, which was reversed by treatment with miR‐26a‐5p mimic or oxymatrine (an inhibitor of TGF‐β1‐Smads pathway). Interestingly, silencing Snhg6 in vivo mitigated BLM‐driven pulmonary fibrosis by regulating the miR‐26a‐5p/TGF‐β1‐Smads axis. Our data revealed that Snhg6 contributed to the process of BLM‐driven lung fibrosis in mouse by modulating the miR‐26a‐5p/TGF‐β1‐Smads axis, suggesting that Snhg6 might be a therapeutic target for lung fibrosis.
Efficient adaptive retrieval and mining in large multimedia databases

Multimedia databases are increasingly common in science, business, entertainment and many other applications. Their size and high dimensionality of features are major challenges for efficient and effective retrieval and mining. Effective similarity models are usually computationally far too complex for straightforward usage in large high dimensional multimedia databases. We propose efficient algorithms for these effective models that show substantially improved scalability. Our index-based methods for efficient query processing and mining restrict the search space to task relevant data. Multistep filter-and-refine approaches using novel filter functions with quality guarantees ensure that fast response times are achieved without any loss of result accuracy. 1 Multimedia Databases There is tremendous growth in multimedia data in application domains such as medicine, engineering, biology and numerous others. New technologies such as computer tomography or digital photography produce increasingly large volumes of data. At the same time, decreasing storage prices allow archiving large amounts of multimedia data at relatively low cost. For database technology, large multimedia databases require efficient and effective strategies for accessing and processing of multimedia data. Examples of typical multimedia content are illustrated in Figure 1: stock data time series, music, medical magnetic resonance images, shape and color images, as well as gene expression data. Traditional database management systems have established themselves as reliable and powerful techniques for archiving and retrieving textual or numeric data. Traditional meta data information, however, is typically not adequate for multimedia applications. For example, users typically search for images not with respect to their size on hard disk, but with respect to content related features like color or shape. Consequently, multimedia database systems should provide content-based access. Likewise, aggregating the content of multimedia databases is not straightforward. Traditional reporting tasks query databases for (aggregated) numbers. Aggregating multimedia objects, or, more precisely, their respective feature values, in such a way is not meaningful. Knowledge discovery techniques aggregate multimedia data with respect to type of media and application focus such that expedient summaries are formed. Multimedia databases thus should provide (1) content-based access, (2) knowledge discov-
Morphology of ctenostome bryozoans: 5. Sundanella, with description of a new species from the Western Atlantic and the Multiporata concept

Ctenostome bryozoans are a small group of gymnolaemates that comprise only a few hundred described species. Soft‐tissue morphology remains the most important source for analysing morphological characters and inferring relationships within this clade. The current study focuses on the genus Sundanella, for which morphological data is scarce to almost absent. We studied two species of the genus, including one new to science, using histology and three‐dimensional reconstruction techniques and confocal laser scanning microscopy. Sundanella generally has a thick, sometimes arborescent cuticle and multiporous interzooidal pore plates. The lophophore is bilateral with an oral rejection tract and generally has 30 or 31 tentacles in both species. The digestive tract shows a large cardia in S. floridensis sp. nov. and an extremely elongated intestine in Sundanella sibogae. Both terminate via a vestibular anus. Only parietodiaphragmatic muscles are present and four to six duplicature bands. Both species show a large broad frontal duplicature band further splitting into four individual bands. The collar is vestibular. Sundanella sibogae shows highly vacuolated cells at the diaphragm, whereas S. floridensis sp. nov. has unique glandular pouches at the diaphragmal area of the tentacle sheath. Such apertural glands have never been encountered in other ctenostomes. Both species of Sundanella are brooders that brood embryos either in the vestibular or cystid wall. Taken together, the current analysis shows numerous characteristics that refute an assignment of Sundanella to victorellid ctenostomes, which only show superficial resemblance, but differ substantially in most of their soft‐body morphological traits. Instead, a close relationship with other multiporate ctenostomes is evident and the families Pherusellidae, Flustrellidrae and Sundanellidae should be summarized as clade ‘Multiporata’ in the future.
A Comprehensive Analysis of Injuries During Army Basic Military Training

ABSTRACT Introduction The injury definitions and surveillance methods commonly used in Army basic military training (BMT) research may underestimate the extent of injury. This study therefore aims to obtain a comprehensive understanding of injuries sustained during BMT by employing recording methods to capture all physical complaints. Materials and methods Six hundred and forty-six recruits were assessed over the 12-week Australian Army BMT course. Throughout BMT injury, data were recorded via (1) physiotherapy reports following recruit consultation, (2) a member of the research team (third party) present at physical training sessions, and (3) recruit daily self-reports. Results Two hundred and thirty-five recruits had ≥1 incident injury recorded by physiotherapists, 365 recruits had ≥1 incident injury recorded by the third party, and 542 recruits reported ≥1 injury-related problems via the self-reported health questionnaire. Six hundred twenty-one, six hundred eighty-seven, and two thousand nine hundred sixty-four incident injuries were recorded from a total of 997 physiotherapy reports, 1,937 third-party reports, and 13,181 self-reported injury-related problems, respectively. The lower extremity was the most commonly injured general body region as indicated by all three recording methods. Overuse accounted for 79% and 76% of documented incident injuries from physiotherapists and the third party, respectively. Conclusions This study highlights that injury recording methods impact injury reporting during BMT. The present findings suggest that traditional injury surveillance methods, which rely on medical encounters, underestimate the injury profile during BMT. Considering accurate injury surveillance is fundamental in the sequence of injury prevention, implementing additional injury recording methods during BMT may thus improve injury surveillance and better inform training modifications and injury prevention programs.
Relative Contributions of Social, Contextual, and Lexical Factors in Speech Processing

This exploratory study examined the simultaneous interactions and relative contributions of bottom-up social information (regional dialect, speaking style), top-down contextual information (semantic predictability), and the internal dynamics of the lexicon (neighborhood density, lexical frequency) to lexical access and word recognition. Cross-modal matching and intelligibility in noise tasks were conducted with a community sample of adults at a local science museum. Each task featured one condition in which keywords were presented in isolation and one condition in which they were presented within a multiword phrase. Lexical processing was slower and more accurate when keywords were presented in their phrasal context, and was both faster and more accurate for auditory stimuli produced in the local Midland dialect. In both tasks, interactions were observed among stimulus dialect, speaking style, semantic predictability, phonological neighborhood density, and lexical frequency. These interactions revealed that bottom-up social information and top-down contextual information contribute more to speech processing than the internal dynamics of the lexicon. Moreover, the relatively stronger bottom-up social effects were observed in both the isolated word and multiword phrase conditions, suggesting that social variation is central to speech processing, even in non-interactive laboratory tasks. At the same time, the specific interactions observed differed between the two experiments, reflecting task-specific demands related to processing time constraints and signal degradation.
Psychometric Properties of the COVID-19 Pandemic Fatigue Scale: Cross-sectional Online Survey Study

Background Pandemic fatigue is defined as feelings of demotivation to follow preventive measures against COVID-19, together with decreased trust in government and frequency of information-seeking behaviors. Objective This study aims to analyze the psychometric properties of the COVID-19–specific pandemic fatigue scale according to classical test theory (CTT) and Rasch model approaches in the general Spanish population. Methods This was a cross-sectional study in a representative sample of 1018 adults who completed an online survey in November 2020 in the framework of the COVID-19 Snapshot Monitoring (COSMO)-Spain project. The assessments included the 6-item COVID-19 Pandemic Fatigue Scale (CPFS) and other COVID-19–related variables: COVID-19 infection, adherence to preventive behaviors, information-seeking behavior, self-efficacy, worry, and cognitive and affective risk perception. Data quality, acceptability, reliability, and validity were analyzed according to CTT, and the fit to the Rasch model, unidimensionality, appropriateness of the response scale, item local independency, reliability (person-separation index [PSI]), and item-person distribution were also calculated. Results The mean CPFS score was 17.06 (SD 5.04, range 6-30), with higher scores for women, younger participants, participants who never seek information on COVID-19, those who think they would contract a mild disease in case of infection, those with higher level of worry about coronavirus/COVID-19, and those who felt depressed or felt the coronavirus/COVID-19 is spreading slowly (all P<.01). The Cronbach alpha for the CPFS was 0.74. In the confirmatory factor analysis, one factor was identified (root mean square error of approximation [RMSEA]=.02; comparative fit index [CFI]=.99; χ25=8.06, P=.15). The CPFS showed good fit to the Rasch model (χ 224=42.025, P=.01, PSI=.642), unidimensionality (binomial 95% CI –.005 to .045), and item local independency. Conclusions Our results suggest that the CPFS has moderate reliability and internal consistency and it is composed of a single dimension. It is a useful tool to ascertain the level of pandemic fatigue in the general population, which may help to guide the communication and information strategies to face the COVID-19 pandemic.
Reduced exercise capacity is associated with left ventricular systolic dysfunction in long‐term survivors of allogeneic hematopoietic stem‐cell transplantation

Exercise intolerance is a common complication in survivors of allogeneic hematopoietic stem‐cell transplantation (allo‐HSCT). The aim of this study was to determine if cardiac function measured with echocardiography is associated with exercise capacity measured with cardio‐pulmonary exercise tests in long‐term survivors treated in their youth with allo‐HSCT.