Miracle-dz/UniFAR-FTUs huggingface dataset:
Unveiling the underpinnings of various non-conventional ELISA variants: a review article

ABSTRACT Introduction Enzyme-linked immunosorbent assay (ELISA) is a key bio-analytical technique used for the detection of a large array of antigenic substances of scientific, clinical, food safety, and environmental importance. The assay primarily involves capturing and detecting target analytes using specific antigen-antibody interactions. The wide usage of ELISA results from its high specificity and reproducibility. Notwithstanding, the conventional microwell plate-based format of ELISA has some major drawbacks, such as long assay time (4–18 h), large sample volumes requirement (100–200 μL), lack of multiplicity, and burdensome procedures that limit its utility in rapid and affordable diagnostics. Areas covered Here, we reviewed microfluidic-ELISA, paper-ELISA, aptamer-ELISA, and those based on novel incubation such as heat-ELISA, pressure-ELISA, microwave-ELISA, and sound-ELISA. Further, the current trends and future prospects of these ELISA protocols in clinical diagnostics are discussed. Expert opinion The reviewed non-conventional ELISA formats are relatively rapid, require low reagent volumes, are multiplexable, and could be performed in a low-cost setup. In our opinion, these non-conventional variants of ELISA are on a par with the conventional format for clinical diagnostics and fundamental biological research and hold added clinical translational potential for quick, inexpensive, and convenient measurements.
The importance of human factors in therapeutic dietary errors of a hospital: A mixed-methods study

An accurate therapeutic diet can help people improve their medical condition. Any discrepancy in this regard could jeopardize a patient’s clinical condition. This study was aimed to determine prevalence of dietary errors among in-patients at an international private hospital’s food department, and to explore causes of error to suggest strategies to reduce such errors in the future. Thus, a sequential explanatory mixed-methods study was carried out. For the quantitative part, secondary data were collected on a daily basis over one-month. For qualitative data, errors arising during the meal flow process were traced to the source on the same day of error followed by qualitative interviews with person responsible. Quantitative data were analyzed in SPSS v.25 as percentages. Qualitative data were analyzed by deductive-inductive thematic analysis. Out of a total of 7041 diets, we found that only 17 had errors. Of these, almost two-thirds were critical. Majority of these errors took place during diet card preparation (52.94%), by dietitians (70.59%), during weekdays (82.35%), breakfasts (47.06%), and in the cardiac care ward (47.06%). The causes identified through interviews were lack of backup or accessory food staff, and employee’s personal and domestic issues. It was concluded that even though the prevalence of dietary errors was low in this study, critical errors formed majority of these errors. Adopting organizational behavior strategies in the hospital may not only reduce dietary errors, but improve patients’ well-being, and employee satisfaction in a long run.
Patient Characteristics and Positive Outcomes Associated With a Novel Youth Inpatient Program for Concurrent Disorders

ABSTRACT Concurrent substance use and mental health disorders in youth are a major public health concern and require specialized and comprehensive services. In this paper, a novel inpatient tertiary care facility serving youth aged 13 to 18 with significant concurrent substance use and mental health issues is introduced. The development of this unit was prompted by the opioid overdose crisis in British Columbia and serves as the third concurrent disorders unit in Canada catered specifically to an adolescent population. From its opening in 2017, preadmission and postadmission data from each patient was gathered with the aim of providing a robust image of the serviced patient population as well as the efficacy of this service model. Patients admitted to this program had significantly higher quality of life (d = 0.65) and significantly lower suicidality (d = 0.86) at discharge, compared with at admission. Patients identifying as female had significantly lower quality of life, higher suicidality, and higher prevalence of adverse childhood events compared with patients identifying as male. Results from this program evaluation outline the efficacy of a novel concurrent disorders program for youth while further providing an overview of clinical and relevant demographic characteristics from an underanalyzed patient population.
Factor structure and psychometric properties of Polish version of Parental Bonding Instrument (PBI) among adults and adolescents

Parental Bonding Instrument (PBI) by Parker et al., is a widely known and used tool in studies on the assessment of parenting behavior in adult, adolescent and child populations. This tool has had many translations and adaptations globally. In Poland, the factor structure and psychometric properties of PBI have not been studied so far. The aim of the presented research was to perform such an analysis both in the group of adults and adolescents. The data from four research projects, in which the 25-item version of the PBI translated into Polish was used, were analyzed. Data from 698 participants in total, including 473 adults and 225 adolescents were collected. Exploratory factor analyzes was performed for both mother and father version. A study of the reliability of individual factors, stability over time (test-retest) and an analysis of criterion validity were carried out. Both in the group of adults and adolescents, obtained a three-factor structure, acceptable reliability and stability over time. Moreover PBI correlated with another Polish tool in line with the adopted hypotheses, showing satisfactory criteria validity.
Leptospirosis with multiple organ dysfunction in a mongoose-scat-detection dog infected with Leptospira interrogans serogroup Hebdomadis, Okinawa, Japan

A 2-year-old male mongoose-scat-detection dog was diagnosed with leptospirosis by urine PCR. The patient developed acute renal failure, hepatic dysfunction, and disseminated intravascular coagulation. Treatment with antibiotics was administered, including ampicillin and doxycycline, and supportive care management was provided. Seroconversion against serogroup Hebdomadis was observed on day 8. The leptospiral gene flaB was detected only in urine collected on day 1, from which Leptospira interrogans ST329 was identified by multilocus sequence typing using seven housekeeping genes. L. interrogans serogroup Hebdomadis ST329 has been isolated from mongooses and humans in Okinawa, Japan. This patient received early treatment with antibiotics, which may have contributed to the early recovery of renal function and removal of L. interrogans from kidney tissue.
A distinct four-value blood signature of pyrexia under combination therapy of malignant melanoma with dabrafenib and trametinib evidenced by an algorithm-defined pyrexia score

Pyrexia is a frequent adverse event of BRAF/MEK-inhibitor combination therapy in patients with metastasized malignant melanoma (MM). The study’s objective was to identify laboratory changes which might correlate with the appearance of pyrexia. Initially, data of 38 MM patients treated with dabrafenib plus trametinib, of which 14 patients developed pyrexia, were analysed retrospectively. Graphical visualization of time series of laboratory values suggested that a rise in C-reactive-protein, in parallel with a fall of leukocytes and thrombocytes, were indicative of pyrexia. Additionally, statistical analysis showed a significant correlation between lactate dehydrogenase (LDH) and pyrexia. An algorithm based on these observations was designed using a deductive and heuristic approach in order to calculate a pyrexia score (PS) for each laboratory assessment in treated patients. A second independent data set of 28 MM patients, 8 with pyrexia, was used for the validation of the algorithm. PS based on the four parameters CRP, LDH, leukocyte and thrombocyte numbers, were statistically significantly higher in pyrexia patients, differentiated between groups (F = 20.8; p = <0.0001) and showed a significant predictive value for the diagnosis of pyrexia (F = 6.24; p = 0.013). We provide first evidence that pyrexia in patients treated with BRAF/MEK-blockade can be identified by an algorithm that calculates a score.
A Prospective Longitudinal Study of Qualitative Disaster Narratives of Highly Trauma-Exposed Survivors of the Oklahoma City Bombing Nearly a Quarter Century Later

Objective: To examine highly trauma-exposed survivors of the 1995 Oklahoma City Murrah Federal Building bombing nearly a quarter century later, focusing on survivors’ immediate personal experiences of it through open-ended narratives. Methods: An original sample of 182 bombing survivors, studied approximately 6 months post bombing, was randomly selected from a state registry of 1,092 bombing survivors, with 71% participation. Of the original 182 bombing survivors, 103 completed the longitudinal follow-up, conducted at a median of 23 years post bombing. Qualitative data for the follow-up study were collected using an expanded version of the Disaster Supplement to the Diagnostic Interview Schedule. Of the original sample, 39 were known to be deceased, 25 could not be located, and 15 declined participation. Results: In all, 12 themes were identified, but just 3 (Locations, Bombing experience, and Initial actions) are detailed here. All survivors were in heavily damaged buildings (about one-half in the Murrah Federal building) or directly outside, and the majority (84%) were injured. They described intense and gruesome experiences of the bombing, difficult efforts to escape to safety and help other survivors, and continuing postbombing experiences once outside. Conclusions: A striking finding was the intensity of the survivors’ memories almost a quarter century after the bombing. Their sensory recollections remained vivid, generally as bright and intense as in earlier reporting periods. It may be that the salience of this extreme event stabilized memories of it yielding such vivid descriptions nearly a quarter century later.
The role of Co2+ cation addition in enhancing the AC heat induction power of (CoxMn1–x)Fe2O4 superparamagnetic nanoparticles

The physical role of magnetically semi-hard Co2+ cation addition in enhancing the AC heat induction temperature (T AC) or specific loss power (SLP) of solid (Co x Mn1−x )Fe2O4 superparamagnetic iron oxide nanoparticles (SPIONPs) was systematically investigated at the biologically safe and physiologically tolerable range of H AC (H AC,safe = 1.12 × 109 A m−1 s−1, f appl = 100 kHz, H appl = 140 Oe (11.2 A m−1)) to demonstrate which physical parameter would be the most critical and dominant in enhancing the T AC (SLP) of SPIONPs. According to the experimentally and theoretically analyzed results, it was clearly demonstrated that the enhancement of magnetic anisotropy (K u )-dependent AC magnetic softness including the Néel relaxation time constant τ N (≈τ eff , effective relaxation time constant), and its dependent out-of-phase magnetic susceptibility χ″ primarily caused by the Co2+ cation addition is the most dominant parameter to enhance the T AC (SLP). This clarified result strongly suggests that the development of new design and synthesis methods enabling to significantly enhance the K u by improving the crystalline anisotropy, shape anisotropy, stress (magnetoelastic) anisotropy, thermally-induced anisotropy, and exchange anisotropy is the most critical to enhance the T AC (SLP) of SPIONPs at the H AC,safe (particularly at the lower f appl < 120 kHz) for clinically safe magnetic nanoparticle hyperthermia.
Development of prediction models for complications after primary total hip and knee arthroplasty: a single-centre retrospective cohort study in the Netherlands

Objective The aim of this study was to develop prediction models for patients with total hip arthroplasty (THA) and total knee arthroplasty (TKA) to predict the risk for surgical complications based on personal factors, comorbidities and medication use. Design Retrospective cohort study. Setting Tertiary care in outpatient clinic of university medical centre. Participants 3776 patients with a primary THA or TKA between 2004 and 2018. Primary and secondary outcome measures Multivariable logistic regression models were developed for primary outcome surgical site infection (SSI), and secondary outcomes venous thromboembolism (VTE), postoperative bleeding (POB), luxation, delirium and nerve damage (NER). Results For SSI, age, smoking status, body mass index, presence of immunological disorder, diabetes mellitus, liver disease and use of non-steroidal anti-inflammatory drugs were included. An area under the receiver operating characteristic curve (AUC) of 71.9% (95% CI=69.4% to 74.4%) was found. For this model, liver disease showed to be the strongest predictor with an OR of 10.7 (95% CI=2.4 to 46.6). The models for POB and NER showed AUCs of 73.0% (95% CI=70.7% to 75.4%) and 76.6% (95% CI=73.2% to 80.0%), respectively. For delirium an AUC of 85.9% (95% CI=83.8% to 87.9%) was found, and for the predictive algorithms for luxation and VTE we found least favourable results (AUC=58.4% (95% CI=55.0% to 61.8%) and AUC=66.3% (95% CI=62.7% to 69.9%)). Conclusions Discriminative ability was reasonable for SSI and predicted probabilities ranged from 0.01% to 51.0%. We expect this to enhance shared decision-making in considering THA or TKA since current counselling is predicated on population-based probability of risk, rather than using personalised prediction. We consider our models for SSI, delirium and NER appropriate for clinical use when taking underestimation and overestimation of predicted risk into account. For VTE and POB, caution concerning overestimation exceeding a predicted probability of 0.08 for VTE and 0.05 for POB should be taken into account. Furthermore, future studies should evaluate clinical impact and whether the models are feasible in an external population.
Clinical features and antibiotic treatment of early-onset neonatal listeriosis

Objective To analyze the clinical features, efficacy of antibiotic treatment, and outcome of neonatal listeriosis. Methods This was a retrospective study that included all neonates diagnosed with listeriosis between January 2010 and December 2021. Results Nine male patients and five female patients were analyzed, including 11 preterm and 3 term infants. The mean gestational age was 34 ± 2.6 weeks (29 + 2–40 + 2 weeks), and the mean birth weight was 2392 ± 603 g (1370–3580 g). The maternal clinical manifestations included fever (13/14 [92.9%]), meconium-stained amniotic fluid (12/14 [85.7%]), and intrauterine fetal distress (11/14 [78.6%]). The neonates presented with fever (14/14 [100%]), generalized maculopapular rash (7/14 [50%]), and convulsions (8/14 [57.1%]). Laboratory tests showed leukocytosis (11/14 [78.6%]), monocytosis (9/14 [64.3%]), elevated C-reactive protein levels (13/14 [92.9%]), and thrombocytopenia (6/14 [42.9%]). Eight patients had central nervous system involvement, and Listeria monocytogenes was isolated from the blood in all cases. Empiric antibiotic therapy consisted of a combination of third-generation cephalosporins and penicillin or vancomycin. Four patients died, and 10 patients were cured. Conclusions Preterm infants were more susceptible to listeria infection than term infants, with most having multiple organ injuries. Combined antibiotic application improved the effectiveness of treatment.
Modelling the HPRT-gene mutation induction of particle beams: systematic in vitro data collection, analysis and microdosimetric kinetic model implementation

Objective. Since the early years, particle therapy treatments have been associated with concerns for late toxicities, especially secondary cancer risk (SCR). Nowadays, this concern is related to patients for whom long-term survival is expected (e.g. breast cancer, lymphoma, paediatrics). In the aim to contribute to this research, we present a dedicated statistical and modelling analysis aiming at improving our understanding of the RBE for mutation induction ( RBEM˜ ) for different particle species. Approach. We built a new database based on a systematic collection of RBE data for mutation assays of the gene encoding for the purine salvage enzyme hypoxanthine-guanine phosphoribosyltransferase from literature (105 entries, distributed among 3 cell lines and 16 particle species). The data were employed to perform statistical and modelling analysis. For the latter, we adapted the microdosimetric kinetic model (MKM) to describe the mutagenesis in analogy to lethal lesion induction. Main results. Correlation analysis between RBE for survival (RBES) and RBEM˜ reveals significant correlation between these two quantities (ρ = 0.86, p < 0.05). The correlation gets stronger when looking at subsets of data based on cell line and particle species. We also show that the MKM can be successfully employed to describe RBEM˜, obtaining comparably good agreement with the experimental data. Remarkably, to improve the agreement with experimental data the MKM requires, consistently in all the analysed cases, a reduced domain size for the description of mutation induction compared to that adopted for survival. Significance. We were able to show that RBES and RBEM˜ are strongly related quantities. We also showed for the first time that the MKM could be successfully applied to the description of mutation induction, representing an endpoint different from the more traditional cell killing. In analogy to the RBES, RBEM˜ can be implemented into treatment planning system evaluations.
Usability and feasibility assessment of a smartphone application (Suhriday) for heart failure self-care remote monitoring in an Indian tertiary health care setting: a pilot mixed-methods study

Background/objectives Remote monitoring as a component of chronic heart failure (CHF) management programmes has demonstrated utility in reducing the risk of rehospitalisation and mortality. There is little evidence on mobile health app facilitated remote monitoring in India. We conducted a pilot usability and feasibility assessment of a smartphone-based application (Suhriday) to remotely monitor patients with CHF. Methods We used a mixed-methods design. Usability testing consisted of the think-aloud approach followed by semistructured in-depth interviews (SSIs) and a satisfaction questionnaire. Feasibility testing was done using acceptability and user satisfaction questionnaires in addition to SSIs. We trained five purposively sampled patients with CHF (based on health literacy and gender) and their caregivers (n=10) in self-care monitoring and app use. Usability was assessed using metrics such as task completion, time required for task completion and user satisfaction using Brooke’s System Usability Scale (SUS). Content analysis of the transcripts with deductive coding was performed for both usability and feasibility interviews. The number and types of medical alerts transmitted through the app were captured and escalated to the treating team. Results Critical tasks involving (1) opening the app and identifying task list, (2) reporting blood pressure, weight, heart rate and fluid intake and (3) reporting symptoms were completed within 60 s by four patients. Median (IQR) SUS score was 85 (75–92.5) indicating high level of usability. There were 62 alerts from four patients over 4 weeks, with 36 (58.1%) excess fluid intake alerts and 16 (25.8%) blood pressure variations being the most frequent. One participant had challenges using the app and was monitored through active phone calls. Conclusion Overall usability and satisfaction with Suhriday were good and we were able to remotely manage patients. However, patients with limited health literacy and those facing technological challenges required active structured telephone support.
Practitioners’ Views on Enabling People With Dementia to Remain in Their Homes During and After Crisis

One way of supporting people living with dementia is assisting them to live in their homes (as opposed to being admitted to hospital or other facility) and providing them with a specialist service that responds to crises. This makes it important to understand how best to organize such crisis response services. This study examines practitioners’ actions to reduce inpatient admissions among this population. Through interviews with healthcare practitioners, we find that practitioners negotiate a complex intersection between (1) what constitutes a crisis in relation to the patient and/or the carer, (2) the demands of building a working relationship with both the patient and their family carers, and (3) ensuring effective communications with social services responsible for long-term community support. Findings suggest that policies aimed at reducing admissions should be based on a model of care that more closely maps practitioners’ relational and bio-medical work in these services.
The Cosmic Origins Spectrograph: NUV Imaging Performance

We describe the COS NUV imaging performance as derived from data taken during the Observatory Verification period after HST Servicing Mission #4. This includes photometric growth curves using data taken with the two available MIRROR settings (MIRRORA and MIRRORB) and the PSA and BOA apertures, throughput and image quality as function of spatial offset from the center of the COS aperture, and a description of the imaging-mode point spread function (PSF). The observed PSF is fully consistent with the model (at good HST focus), except for two minor ghosts located about 20 pixels away from the PSF center. A comparison of imaging observations of a cool and a hot flux standard star reveals a significantly higher red sensitivity of the COS imaging modes than that predicted from ground calibration data. Apart from that, the results are roughly consistent with predictions from ground calibration. The new throughput curves are used in the COS imaging and imaging target acquisition exposure time calculators since October 7, 2009.
Movement Velocity as an Indicator of Mechanical Fatigue and Resistance Exercise Intensity in Cross Modalities

ABSTRACT Purpose: This study analyzed the acute mechanical response to three workouts of the day (WOD) protocols in as many repetitions as possible (AMRAP), every minute on the minute (EMOM), and for time (FT) models by quantifying the degree of mechanical fatigue induced by popular resistance exercises in the Cross modalities, front squat (FS), and shoulder press (SP). We also analyzed whether the exercises’ fastest velocity (Vfastest) could be an objective indicator of relative intensity (%1RM). Methods: Nine trained men performed three FS and SP exercises protocols. The degree of fatigue was quantified by the velocity loss (VL) achieved in both exercises and the velocity loss achieved in the WOD (VLWOD). Results: The VLWOD in the AMRAP, EMOM, and FT protocols was 73.2 ± 10.9%, 61.6 ± 15.1%, and 76.1 ± 8.8%, respectively. In the AMRAP and FT protocol, the Vfastest showed very strong relationships with the %1RM for FS and SP (r = −0.83, −0.75, respectively, p < .01); while in the EMOM protocol, there was a strong relationship between these variables, only for the SP (r = −0.61, p < .05). In the FT protocol, we observed an extremely strong relationship for FS (r = −0.91, p < .001) and very strong (r = −0.71, p < .05) for SP between these variables. Conclusion: Therefore, the AMRAP and FT training models induce the highest degrees of mechanical fatigue in the FS and SP exercises, and the Vfastest is a reliable tool for estimating relative intensity in resistance exercises of Cross modalities.