Reproductive Disorders and its Relationship with Hormones and Mineral Status in Bovines of Organized Dairy Farms

In the present study, relationship of reproductive disorders with mineral and hormone status in bovines was analyzed. Paired sera samples (160) were collected randomly from four organized dairy farms in Karnataka (Bijapur, Dharwad) and Tamilnadu (Chennai, Cuddalore). Serum estradiol, progesterone in healthy and RP animals were 11.33±0.58 and 5.67±0.10pg/ml, 1.70±0.08 and 0.77±0.04IU/ml, respectively and significant (P<0.05) decrease in RP bovines. Serum copper, zinc, calcium, magnesium, phosphorus in healthy and RP bovines were 1.29±0.03 and 0.91±0.03ppm, 1.09±0.04 and 0.87±0.03ppm, 9.98±0.17 and 9.07±0.21mg/dl, 3.07±0.16 and 2.36±0.08mg/dl, 1.62±0.08 and 1.52±0.06mg/dl, respectively. Significant (P<0.05) correlation between copper and hormones in bovines of organized dairy farms was observed. Logit analysis revealed significant (P<0.05) positive relationship between estradiol, progesterone and zinc levels with animal health status. Thus, supplementation of these microminerals will reduce the incidence of reproductive disorders in animals and also improves the productivity and profitability of dairy farmers in India. collected from four dairy farms for the study. The blood samples were collected by using serum vacutainer tubes coated with silicone and micronized silica particles to accelerate clotting and multi sample vacutainer needles (Becton and Dickenson Company, USA). The serum was separated by centrifugation at 2000 rpm for 20 minutes. All serum samples were stored at -20° C until used for estimation. Samples from female animals were collected for this study. Based on breeds of cattle, Jersey cross (58), Holstein Friesian cross (47), Sahiwal (9), Deoni (5), Rathi (5), Tharparkar (5) and among buffalo breeds, Murrah (31) were tested. The animals were classified based on age, by the number of lactations as 1 calving (35), 2 calvings (55), 3 calvings (48), 4 calvings (14) and 5 calvings (8). Based on animal health status, the animals were categorised as apparently healthy (63), pregnant (17), repeat breeding (58), abortions (13), metritis (5) and mastitis (4). was looking normal and without any clinical signs; Reproductive problems-Animals with history of abortion, repeat breeding, metritis and mastitis The serum copper showed significant (P<0.05) decrease in reproductive problem animals in all the four farms studied. Zinc concentration in serum revealed significant (P<0.05) decrease in three farms but not in Dharwad farm. The serum calcium levels revealed no significant difference between the healthy and reproductive problem animals except Dharwad farm. Phosphorus levels showed no significant difference but farm in Chennai had significant difference between the two categories of animals. The magnesium concentration showed no significant difference between the apparently healthy and reproductive problem animals in Bijapur and Chennai farms but significant (P<0.05) difference in Dharwad and Cuddalore farms were observed. Overall, there was decrease in the levels of hormones and minerals in animals with reproductive problems when compared to apparently healthy animals were observed indicating their importance in reproduction of bovines.