Miracle-dz/UniFAR-FTUs huggingface dataset:
Reducing the grid orientation dependence of flow routing on square-grid digital elevation models

ABSTRACT Topographical parameters derived from digital elevation models by employing flow routing algorithms may depend on the orientation of the square grid. Grid orientation dependence results from the insufficient isotropy of square grid and affects the flow directions and subsequent calculations based on flow routing. In this article, a systematic approach for analysing the rotational invariance of flow accumulation calculations is presented and applied. Computed flow accumulation maps are found to depend strongly on the grid orientation, especially if flow routing methods with low dispersion are used. It is also shown that isotropy of flow routing algorithms can be significantly improved by introducing a numerical parameter resulting in adjustable weighting for cardinal and diagonal directions in flow routing. The actual value of this parameter depends on the used flow routing method.
Attitudes and emotions towards pain and sensitivity to painful stimuli among people routinely engaging in masochistic behaviour

People engaged in masochistic behaviour (MB) seek to experience pain and the pleasure it evokes in sadomasochistic (S&M) sessions. The sensitivity and attitude to pain in these individuals has hardly been tested. We evaluated pain perception among these individuals and tested whether their experiences and attitudes towards pain are context‐related.
Basic parameters of spontaneous speech as a sensitive method for measuring change during the course of aphasia.

BACKGROUND Spontaneous speech of aphasic persons is often scored on rating scales assessing aphasic symptoms. Rating scales have the advantage of an easy and fast scoring system, but might lack sensitivity. Quantitative analysis of either aphasic symptoms or basic parameters provides a useful alternative. Basic parameters are essential units of language like word categories or syntactic completeness and can be identified in both impaired and unimpaired adult and child language. AIMS To examine whether basic parameters of spontaneous speech are more sensitive to change during the course of recovery from aphasia than conventional spontaneous speech rating scales. METHODS & PROCEDURES Spontaneous speech samples of 28 aphasic participants were analysed using a quantitative computer-assisted method as well as conventional spontaneous speech rating scales before and after 7 weeks of intensive language treatment. The analysis focused on the following basic parameters: percentage words, percentage open class words, syntactic completeness, complexity, and mean length of utterances. The participants were also tested with the Aachen Aphasia Test before and after treatment. OUTCOME & RESULTS Significant change in at least one basic parameter was observed in 20 participants, while only four participants showed significant change in one of the spontaneous speech rating scales. CONCLUSIONS In comparison with conventional spontaneous speech rating scales, the basic parameters proved to be more sensitive to change. For the time being, however, some limitations remain with regard to the specificity of the basic parameters. Thus, additional data are needed to provide further support of the clinical significance of the measured changes.
Added sugars and nutrient density in the diet of Danish children

The relation between added sugars and density of dietary fibre and micronutrients was examined by analysing diets from 983 Danish girls and boys aged 4-14 years. The average intake of added sugars was 13.7 E% ranging from 3.4 to 38 E%. One fourth of the diets were below the recommended maximum level of 10 E%. No differences between sex and age groups were found. The diets were ranked and divided into quintiles (fifths) according to percentage of energy from added sugars. A strongly significant decline in nutrient density for all nutrients, except vitamin C, was observed as sugar concentration rose across quintiles. Nutrient densities expressed relative to recommended values varied from 30% to 300% illustrating that the nutritional significance of the dilution effect of added sugars differs from one nutrient to another. The results support the concept of "empty calories" and restriction of added sugars should still be recommended. Key words: Added sugars, children, empty calories, micronutrients, nutrient density
Aspectos clínicos, ultra-sonográficos e venográficos da tromboflebite jugular experimental em equinos

Jugular thrombophlebitis is a common complication of disease processes associated with repeated venipuncture, injection of irritant solutions, and the use of indwelling catheters, especially with bacterial contamination. Bilateral thrombophlebitis may result in edema of the soft tissues of the head, reduction of athletic performance and even death of the animal. This disease, although common in horses, is not much known regarding its evolution and treatment. The aim of this study was to evaluate the clinical and structural changes of experimentally induced jugular thrombophlebitis in horses, through clinical examination, ultrasound and venography of the thrombus and the vessel, verifying the possibility of thrombus recanalization and compensatory produced blood flow. The jugular thrombophlebitis was induced unilaterally into 5 horses, monitored by clinical (general, regional and local) and ultrassonographycs exams. Venographs were made at pre-induction, induction and every 6 days after induction of thrombophlebitis, in order to observe recanalization of the occlusive thrombus and presence of blood vessels in the drainage allowance. Occurrence of moderate edema was observed in the parotid, masseter and supra orbital regions, and mild edema in the submandibular region. The jugular engorgement of the cranial region of induction persisted throughout the period of evaluation. The caudal portion to the thrombophlebitis showed engorgement with compression on the vein at the thorax entrance since the first day after induction. The ultrasound examinations showed total occlusive thrombus formation of 3 animals, partial recirculating flow in the jugular vein in 2 animals, and collateral blood vessels from the cranial obstruction to the caudal portion. The venography revealed normal linear blood flow in the preoperative and occlusive thrombus with contrast directed filling of the vessels to the compensatory portion caudal to the vein occlusion or cranial to the thrombus in the postoperative moments. After vein resection of the segment containing the thrombus, the cephalic edema was less intense than after the induction of the thrombophlebtits. The ultrassonography and venography post resection showed vascularity increase in this region. It was concluded that there is recanalization with endothelialization and vascular compensation made by pre-existing vessels necessary for drainage.
Exploring possible mechanisms for primary bile acid malabsorption: evidence for different regulation of ileal bile acid transporter transcripts in chronic diarrhoea

Objectives Chronic diarrhoea resulting from primary idiopathic bile acid malabsorption (IBAM) is common, but its aetiology is largely unknown. We investigated possible mechanisms, first looking for common sequence variations in the cytoplasmic ileal bile acid-binding protein (IBABP, gene symbol FABP6), and secondly, determining the expression of ileal mucosal transcripts for the apical sodium-linked bile acid transporter (ASBT), IBABP, the putative basolateral transporters, OST&agr; and OST&bgr;, and regulatory factors. Methods Genomic DNA was prepared from two cohorts of patients and two control groups; the promoter and exonic regions of FABP6 were sequenced. In intestinal biopsies, transcript expression was measured by quantitative real time-PCR, using ileum from 17 patients and 21 controls. Results Sequence variations were identified in FABP6, but overall frequencies were similar in patients and controls. Transcripts of ASBT and IBABP, but not OST&agr; and OST&bgr;, were expressed at higher levels in ileum than duodenum. The transcription factors farnesoid-X-receptor (FXR) and liver-receptor-homologue (LRH-1) were also more abundant in ileum, as was fibroblast growth factor 19 (FGF19), unlike short heterodimer partner (SHP), c-Fos, or CDX2. No significant differences in mean or median values were found between the groups for any of these transcripts. However, findings on regression analysis suggested that these transporters differ in their regulation, particularly in the relationships of CDX2, LRH-1 and FXR with OST&agr;. Conclusion Most cases of IBAM are unlikely to be caused by genetic variation in FABP6 or by major differences in transporter transcript expression. Our evidence indicates that other factors, such as regulation of expression of the basolateral bile acid transporter, should be considered as possible causes.
Effects of pregabalin on the nociceptive, emotional and cognitive manifestations of neuropathic pain in mice

Preclinical drug discovery for the treatment of chronic pain is at present challenged by the difficulty to study behaviours comparable to the complex human pain experience in animals. Several reports have demonstrated a frequent association of chronic pain in humans with affective disorders, such as anxiety and depression, and impaired cognitive functions, including memory and decision making, and motivation for goal‐directed behaviours. In this study, we validated different behavioural outcomes to measure the emotional and cognitive manifestations of neuropathic pain induced in mice by partial sciatic nerve ligation.
Treatment of preestablished diet-induced fatty liver by oral fatty acid–bile acid conjugates in rodents

Background Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in industrialized countries. It has no accepted medical therapy. Fatty acid–bile acid conjugates (FABACs) were proven to prevent diet-induced NAFLD in rodents. Aim This study was undertaken to test whether oral FABACs are also effective in reducing liver fat in preestablished diet-induced NAFLD. Methods NAFLD was induced in mice and rats by a high-fat diet and maintained by various proportions thereof. The FABACs used were conjugates of cholic acid with either arachidic or stearic acids. Results FABAC therapy reduced liver fat in all four series of experiments. The rapidity of the effect was inversely proportional to the concentration of fat in the maintenance diet. In mice on a 25% maintenance diet FABACs decreased total liver lipids by about 30% in 4 weeks (P<0.03). Diglycerides (P<0.003) and triglycerides (P<0.01) were the main neutral liver lipids that decreased during FABAC therapy. Both FABACs tested reduced liver fat in NAFLD at doses of 25 and 150 mg/kg/day. High-fat diet increased, whereas FABAC therapy decreased plasma 16 : 1/(16 : 0+16 : 1) fatty acid ratio – a marker of stearoyl CoA desaturase activity. In HepG2 cells FABACs decreased de-novo fatty acid synthesis dose dependently. Conclusion Oral FABAC therapy decreased liver fat in preestablished NAFLD in mice and rats. Inhibition of stearoyl CoA desaturase activity and fatty acid synthesis are mechanisms that may contribute to this decrease. FABACs may be potential therapeutic agents for human NAFLD.
Electrochemical techniques for microfluidic applications

Electrochemical principles provide key techniques to promote the construction of bio/chemical microsystems of the next generation. There is a wealth of technology for the microfabrication of bio/chemical sensors. In addition, microfluidic transport in a network of flow channels, pH regulation, and automatic switching can be realized by electrochemical principles. Since the basic components of the devices are electrode patterns, the integration of different components is easily achieved. With these techniques, bio/chemical assays that require the exchange of solutions can be conducted on a chip. Furthermore, autonomous microanalysis systems that can carry out necessary procedures are beginning to be realized. In this article, techniques developed in our group will be comprehensively introduced.
Sexuality, gendered identities and exclusion: the deployment of proper (hetero)sexuality within an HIV-prevention text from South Africa

HIV prevention discourses concern lives, the protection of bodily rights and people's active involvement in the policies and programmes that affect them. HIV prevention discourses also create lives, relying upon the deployment of normative sexual identities at the same time as they invite complex and fluid youth identities to embody the norms of prevention. This paper examines a particular HIV prevention text that is available to teachers in the Western Cape province of South Africa to support the implementation of the national Life Orientation programme. Rather than considering this text as a neutral ‘scaffold’ upon which teachers and students add cultural meanings, it is important to interrogate the ways in which texts rely upon and reiterate particular discursive constructions of the youth sexual subject. This paper argues that the text deploys a particular discursive framework in order to construct a ‘normal’ (and hetero) sexuality that validates, rather than questions, social constructions of masculine privilege within heterosexuality. This is achieved through the deployment of a scientific expertise of sexuality; the mobilisation of a valued hetero/homosexual binary to create a ‘safe’ heterosexuality; the normalisation of bourgeois sexuality through the ideology of marriage; and the naturalisation of heterosexual masculine and feminine identities.
Genomic screening for Chlamydophila pneumoniae-specific antigens using serum samples from patients with primary infection.

Chlamydophila pneumoniae, an obligate intracellular human pathogen, causes respiratory tract infections. The most common techniques used for the serological diagnosis of C. pneumoniae infections are microimmunofluorescence tests and commercial serological ELISA tests; these are based on the detection of antibodies against whole chlamydial elementary bodies and lipopolysaccharide/outer membrane protein, respectively. Identification of more specific and highly immunodominant antigens is essential for the development of new serodiagnostic assays. To identify novel specific antigens from C. pneumoniae, we screened 455 genes with unknown function in the genome of C. pneumoniae J138. Extracts of Saccharomyces cerevisiae cells expressing GFP-tagged C. pneumoniae proteins were subjected to Western blot analysis using serum samples from C. pneumoniae-infected patients as the primary antibodies. From this comprehensive analysis, 58 clones expressing C. pneumoniae open reading frames, including hypothetical proteins, were identified as antigens. These results have provided useful information for the development of new serological tools for the diagnosis for C. pneumoniae infections and for the development of vaccines in future.
Old ideals and new realities: the changing context of young people's partnerships in Cebu, Philippines

The Philippines has experienced rapid sociodemographic changes in recent years, with implications for young people. This study combines quantitative and qualitative data from Metro Cebu to assess the timing and predictors of young people's partnerships, as well as the context in which these partnerships are occurring. The majority of young people (54%) had premarital sex, though this pattern varied by gender. Wealthier, urban young men, and women with less education and lower reported religiosity, were more likely to have premarital sex. Engagement in risk behaviours was predictive of premarital sex for both males and females. The qualitative data contextualise the circumstances under which young people engage in sex and form partnerships and illustrate how sociocultural norms contribute to gender differences in partnership patterns. Given the ‘new’ realities of young Filipinos' lives, targeted efforts to support the transition to adulthood are needed to avert potentially adverse life events.
Computerized case-based testing: A modern method to assess clinical decision making

This article describes an assessment system that has been developed to focus on application of knowledge. Its two major features are case-based testing and the use of multimedia and computer tools. The case material for this testing type is based on the key-features concept, reporting the most relevant characteristics of a case and asking a limited number of questions, each aimed at essential decisions. These cases are produced in daily practice using real patients. Subsequently an extensive review process is used to check for flaws in description, phrasing or answer keys. Cases are stored in an item-bank out of which an automated random stratified selection can be drawn, according to a pre-specified blueprint. Cases are then presented to the students by way of a specially developed interface using multimedia when indicated. The article further describes advantages, disadvantages and experiences.
Glycosylation analysis of glycoproteins and proteoglycans using capillary electrophoresis‐mass spectrometry strategies

This review highlights recent developments in glycosylation analysis by modern MS in combination with CE based preseparation. Focused on CE‐MS strategies aimed for glycotyping, the review addresses the detailed glycoform analysis of glycoproteins, glycopeptides, and proteoglycans. Glycoform analysis in the context of modern glycoproteomics is briefly addressed, as well. CZE, CIEF, and frontal analysis CE approaches hyphenated to high‐resolution multistage MS for the detailed analysis of protein‐linked glycan structures are overviewed in a comprehensive way. Advantages and limitations of various methodological approaches and techniques as well as mass spectrometric instrumentation are discussed in the particular context of glycoanalysis.
Women's perceptions of how pregnancy influences the context of intimate partner violence in Germany

Intimate partner violence during pregnancy is receiving increased attention because of its high prevalence and health effects. Still, little is known about women's perceptions on how their pregnancy influences the context in which intimate partner violence occurs. We conducted 19 in-depth interviews with women who had experienced intimate partner violence around the time of pregnancy. Women clearly perceived pregnancy as a turning point, because it created new expectations and a feeling of being overwhelmed. This led to violence by reducing women's acceptance of their partner's unemployment, alcohol abuse and lack of relationship commitment, or by increasing women's vulnerability because they felt too young to raise a child alone. Pregnancy also led to violence by bringing back childhood memories or by taking attention away from their partners. Understanding how pregnancy influences the context in which intimate partner violence occurs is important to provide abused, pregnant women with the services they need.
Condom use and intimacy among Tajik male migrants and their regular female partners in Moscow

This study examined condom use and intimacy among Tajik male migrants and their regular female partners in Moscow, Russia. This study included a survey of 400 Tajik male labour migrants and longitudinal ethnographic interviews with 30 of the surveyed male migrants and 30 of their regular female partners. of the surveyed male migrants, 351 (88%) reported having a regular female partner in Moscow. Findings demonstrated that the migrants' and regular partners' intentions to use condoms diminished with increased intimacy, yet each party perceived intimacy differently. Migrants' intimacy with regular partners was determined by their familiarity and the perceived sexual cleanliness of their partner. Migrants believed that Muslim women were cleaner than Orthodox Christian women and reported using condoms more frequently with Orthodox Christian regular partners. Regular partners reported determining intimacy based on the perceived commitment of the male migrant. When perceived commitment faced a crisis, intimacy declined and regular partners renegotiated condom use. The association between intimacy and condom use suggests that HIV-prevention programmes should aim to help male migrants and female regular partners to dissociate their approaches to condom use from their perceptions of intimacy.
Risk factors for long-term prognosis in hepatocellular carcinoma after radiofrequency ablation therapy: the clinical implication of aspartate aminotransferase–platelet ratio index

Objective Percutaneous radiofrequency ablation (RFA) is one of the first-line curative therapies for hepatocellular carcinoma (HCC), but factors affecting the prognosis remain unclear. This study aimed to evaluate the prognostic factors associated with the prognosis in patients with HCC undergoing RFA. Methods The study enrolled 190 treatment-naïve patients with HCC (<5 cm). Factors were analyzed in terms of overall survival and recurrence. Results After a median follow-up of 30.7±17.5 months, 41 patients died and 149 patients were alive. Of the 112 patients who developed tumor recurrence, 61 had local recurrence and 51 had distant intrahepatic recurrence. The 5-year overall survival rate and recurrence rate were 65.5 and 73.6%, respectively. Factors associated with overall mortality were, age older than 65 years (P=0.019), aspartate aminotransferase-to-platelet ratio index (APRI) of more than 1 (P=0.015), prothrombin time international normalized ratio of more than 1.1 (P=0.013), multinodularity (P=0.024), and Child–Pugh grade B (P=0.036). Besides, multivariate analysis disclosed that APRI of more than 1 (P=0.002), multinodularity (P<0.001), and tumor size of more than 2 cm (P=0.002) predicted higher incidence of developing recurrence after RFA. Factors determining local recurrence were, age older than 65 years (P=0.030), APRI of more than 1 (P=0.003), multinodularity (P=0.019), and tumor size of more than 2 cm (P=0.015), whereas only APRI of more than 1 (P=0.013) and multinodularity (P<0.001) were independent risk factors predictive of intrahepatic distant metastasis. Conclusion Both multinodularity and APRI are associated with overall survival and recurrence for patients with HCC after RFA therapy. Consequently, APRI seems to serve as a feasible marker for predicting the prognosis of patients with small HCC undergoing RFA.
Parkin-mediated ubiquitination regulates phospholipase C-γ1

Mutations in parkin cause autosomal recessive forms of Parkinson’s disease (PD), with an early age of onset and similar pathological phenotype to the idiopathic disease. Parkin has been identified as an E3 ubiquitin ligase that mediates different types of ubiquitination, which has made the search for substrates an intriguing possibility to identify pathological mechanisms linked to PD. In this study, we present PLCγ1 as a novel substrate for parkin. This association was found in non‐transfected human neuroblastoma SH‐SY5Y cells as well as in stable cell lines expressing parkin WT and familial mutants R42P and G328E. Analysis of cortical, striatal and nigral human brain homogenates revealed that the interaction between parkin and PLCγ1 is consistent throughout these regions, suggesting that the interaction is likely to have a physiological relevance for humans. Unlike many of the previously identified substrates, we could also show that the steady‐state levels of PLCγ1 is significantly higher in parkin KO mice and lower in parkin WT human neuroblastoma cells, suggesting that parkin ubiquitination of PLCγ1 is required for proteasomal degradation. In line with this idea, we show that the ability to ubiquitinate PLCγ1 in vitro differs significantly between WT and familial mutant parkin. In this study, we demonstrate that parkin interacts with PLCγ1, affecting PLCγ1 steady state protein levels in human and murine models with manipulated parkin function and expression levels. This finding could be of relevance for finding novel pathogenic mechanisms leading to PD.