Miracle-dz/UniFAR-FTUs huggingface dataset:
Oral health literacy and socio-demographics as determinants of oral health status and preventive behavior measures in participants of a pre-marriage counseling program

This analytical cross-sectional study aimed to assess the correlation of oral health literacy (OHL) and oral health status and behavior in participants of a pre-marriage counseling program. Also, it aimed to determine the target population’s OHL needs to design necessary interventions. A total of 828 couples with a mean age of 24.22 years participating in a pre-marriage counseling program were evaluated. Data were collected using the oral health literacy-adult questionnaire (OHL-AQ). Clinical oral examinations were performed to measure the gingival index (GI), plaque index (PI), and DMFT. The mean OHL score of the couples was 7.86±3.83 out of 17; while, the mean DMFT score was 6.30±5.73. After controlling for the confounders, OHL had a significant correlation with the frequency of dental visits, and smoking status, and inverse correlation with the number of decayed teeth and missing teeth, and PI, and GI (p<0.05). The current results highlight the necessity of OHL interventions to fill the existing gaps. This is an unmet need of the couples, and such interventions are required to promote their own oral health as well as the oral health of their future children.
Changing the pattern of the back-muscle flexion–relaxation phenomenon through flexibility training in relatively inflexible young men

Although several studies have investigated the back-muscle flexion–relaxation phenomenon (FRP), the effect of individual flexibility on the FRP has been discussed infrequently, with very limited data on the influence of flexibility training on the FRP. This study thus examined the effect of flexibility training on the change of back-muscle FRP pattern in relatively inflexible young men. We collected and analyzed the valid data from 20 male participants (10 each with high and low flexibility included in the control and trained groups, respectively) when flexing their trunks at seven trunk flexion positions (0°–90°, in increments of 15°); their erector spinae and hamstring activation, pelvic tilt, and lumbosacral angle were then recorded. After 7 weeks of flexibility training for the low-flexibility group, no difference in flexibility was discovered between this group and the control (originally high-flexibility) group. The trunk flexion experiment was then repeated. The results showed that before the training stage, the low-flexibility group had lower erector spinae and higher hamstring activation, a larger pelvic tilt, and a smaller lumbosacral angle. By contrast, after training, the erector spinae and hamstring activation, pelvic tilt, and lumbosacral angle were significantly changed, and no intergroup differences were observed in FRP patterns. The study results suggest that flexibility training changes lumbopelvic movement and thereby reduces the degree of the back-muscle FRP when trunk flexion is performed.
Control system for the switched reluctance drive of a flywheel energy‐storage module

This paper presents a novel control system for the operation of a flywheel energy-storage (FES) module used for power peak shaving in high-speed railway substations. A double power converter drives a switched reluctance machine (SRM) along with a flywheel operating in a wide speed range. An innovative controller architecture based on modular microcontrolled boards connected through a CAN network is introduced. Standard switching patterns are modified to reduce iron and semiconductor losses and to improve harmonic performance. Experimental results for a 140 kW/5 MJ prototype are presented confirming controller compliance with the system specifications and requirements. Copyright © 2007 John Wiley & Sons, Ltd.
Towards theory building in manufacturing flexibility

This study consolidates and extends previous research to propose a theoretical framework of manufacturing flexibility. The framework proposes antecedent roles for manufacturing flexibilities in supporting a range of agility-related manufacturing priorities; suggests how such manufacturing flexibilities can be developed; and relates the effects of alignment between flexibility-facilitated manufacturing outcomes and business strategy on firm performance. A questionnaire was administered to 1700 senior level managers in manufacturing firms, with 322 valid surveys returned for data analysis. The results support the framework, and suggest that different manufacturing priorities can be facilitated through the development of specific manufacturing flexibilities. The results also indicate that these manufacturing flexibilities can be acquired through the development of purchasing competence in a firm. The data provide partial support for a relationship between congruity in manufacturing and business goals, and firm performance.
Reliability issues of Gallium Nitride High Electron Mobility Transistors

In the present paper we review the most recent degradation modes and mechanisms recently observed in AlGaN/GaN (Aluminum Gallium Nitride/Gallium Nitride). High Electron-Mobility Transistors (HEMTs), as resulting from a detailed accelerated testing campaign, based on reverse bias tests and DC accelerated life tests at various temperatures. Despite the large efforts spent in the last few years, and the progress in mean time to failure values, reliability of GaN HEMTs, and millimeter microwave integrated circuits still represent a relevant issue for the market penetration of these devices. The role of temperature in promoting GaN HEMT failure is controversial, and the accelerating degradation factors are largely unknown. The present paper proposes a methodology for the analysis of failure modes and mechanisms of GaN HEMTs, based on (i) DC and RF stress tests accompanied by an (ii) extensive characterization of traps using deep level transient spectroscopy and pulsed measurements, (iii) detailed analysis of electrical characteristics, and (iv) comparison with two-dimensional device simulations. Results of failure analysis using various microscopy and spectroscopy techniques are presented and failure mechanisms observed at the high electric field values typical of the operation of these devices are reviewed.
Improvement of sensing and actuating property of parallel beam gyroscope

Parallel beam structure has many significant features as follows: the tip moves in parallel at the end; the structure converts small displacements into large strain; strain is concentrated at the boundary point between the parallel-beam part and the supporting part; interfere between the units is reduced; lateral rigidity is high. The authors propose this structure for the development of a piezoelectric vibrating gyroscope (angular rate sensor), and detection of the angular rate using this gyroscope. The method for super sensitizing by bringing two resonance frequencies (drive resonance frequency and sensing resonance frequency) close, is presented. A suppression method for mechanical coupling that arises by bringing two resonance frequencies close, is described. It is concluded that the structure described is suitable for the gyroscope.
Monitoring RNA dynamics in native transcriptional complexes

Significance Transcription of DNA into RNA is crucial to life, and understanding RNA polymerase (RNAP) function has received considerable attention. In contrast, how the nascent RNA folds into structures that impact transcription itself and regulate gene expression remains poorly understood. Here, we combine single-molecule Förster resonance energy transfer and site-specific fluorescent labelling of transcripts within native complexes to enable real-time cotranscriptional folding studies of a metabolite-sensing riboswitch from Escherichia coli. By monitoring the folding of riboswitches stalled at RNAP pausing sites and during active elongation, we reveal a crucial role for RNAP, which directs RNA folding to allow thiamin pyrophosphate sensing within a precise, transcriptional hotspot. Our approach offers a unique opportunity to unveil cotranscriptional processes in eukaryotic and bacterial systems. Cotranscriptional RNA folding is crucial for the timely control of biological processes, but because of its transient nature, its study has remained challenging. While single-molecule Förster resonance energy transfer (smFRET) is unique to investigate transient RNA structures, its application to cotranscriptional studies has been limited to nonnative systems lacking RNA polymerase (RNAP)–dependent features, which are crucial for gene regulation. Here, we present an approach that enables site-specific labeling and smFRET studies of kilobase-length transcripts within native bacterial complexes. By monitoring Escherichia coli nascent riboswitches, we reveal an inverse relationship between elongation speed and metabolite-sensing efficiency and show that pause sites upstream of the translation start codon delimit a sequence hotspot for metabolite sensing during transcription. Furthermore, we demonstrate a crucial role of the bacterial RNAP actively delaying the formation, within the hotspot sequence, of competing structures precluding metabolite binding. Our approach allows the investigation of cotranscriptional regulatory mechanisms in bacterial and eukaryotic elongation complexes.
The role of vitamin D in prevention of COVID-19 and its severity: an umbrella review

Background and aim: Vitamin D is known to modulate immune response and its deficiency was associated with respiratory distress in patients hospitalized for pneumonia. Nevertheless, numerous reviews on vitamin D in COVID-19 patients have shown conflicting results, as previously reported also for other respiratory diseases (e.g., influenza). Methods: This umbrella review aims to assess whether low serum 25-OHD is associated with susceptibility to COVID 19, their severity, and mortality. A total of 1559 studies were excluded after the title, abstract and full-text articles screening and 9 papers were included in this review: 2 systematic reviews and 7 metanalysis. Results: The findings of this review that summarized studies from 5 WHO regions (European Region, Region of the Americas, South-East Asia Region, Eastern Mediterranean Region, Western Pacific Region) to exclusion only African region, show that low serum 25-OHD levels are associated with higher infection risks for COVID-19. Conclusions: Although the umbrella findings indicate a potential role of vitamin D deficiency in COVID-19 severity in hospitalized patients and showing an association between Vitamin D supplementation and COVID-19 severity, however, more robust data from randomized controlled trials are further needed to confirm a possible association with the mortality rates. (www.actabiomedica.it)
Anatomical description of the origin and distribution of the brachial plexus to the antebrachium in one puma (Puma concolor) (Linnaeus, 1771)

The knowledge of the anatomy of the brachial plexus in animals is of great importance due to its applicability in clinical, diagnostic and surgical procedures. The objective of the present study was to describe the anatomy of the brachial plexus in the puma. The results demonstrate a broad anatomical similarity with other felines; however, some differences were found. The formation of the brachial plexus in the puma occurred from the ventral interconnections of the last three cervical nerve segments and the first thoracic (C6, C7, C8 and T1). The N. suprascapularis emerges from C6, innervating the M. supraspinatus, the M. infraspinatus, and also the M. cleidobrachialis, the latter by a smaller branch. We found an independent branch emerging from C6 that innervates the M. serratus ventralis cervicis, not reported in other species. The innervation territory of the N. axillary includes the M. cleidobrachialis. The M. teres major was not innervated by the axillary nerve, but by an entirely independent branch that came from C6 and C7, and that also innervated the most caudal part of the M. subscapularis.
A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

Abstract The hemibiotrophic pathogen Bipolaris sorokiniana causes root rot, leaf blotching, and black embryos in wheat and barley worldwide, resulting in significant yield and quality reductions. However, the mechanism underlying the host–pathogen interactions between B. sorokiniana and wheat or barley remains unknown. The B. sorokiniana genome encodes a large number of uncharacterized putative effector proteins. In this study, we identified a putative secreted protein, CsSp1, with a classic N‐terminal signal peptide, that is induced during early infection. A split‐marker approach was used to knock out CsSP1 in the Lankao 9‐3 strain. Compared with the wild type, the deletion mutant ∆Cssp1 displayed less radial growth on potato dextrose agar plates and produced fewer spores, and complementary transformation completely restored the phenotype of the deletion mutant to that of the wild type. The pathogenicity of the deletion mutant in wheat was attenuated even though appressoria still penetrated the host. Additionally, the infectious hyphae in the deletion mutant became swollen and exhibited reduced growth in plant cells. The signal peptide of CsSp1 was functionally verified through a yeast YTK12 secretion system. Transient expression of CsSp1 in Nicotiana benthamiana inhibited lesion formation caused by Phytophthora capsici. Moreover, CsSp1 localized in the nucleus and cytoplasm of plant cells. In B. sorokiniana‐infected wheat leaves, the salicylic acid‐regulated genes TaPAL, TaPR1, and TaPR2 were down‐regulated in the ∆Cssp1 strain compared with the wild‐type strain under the same conditions. Therefore, CsSp1 is a virulence effector and is involved in triggering host immunity.
Running to save sight: The effects of exercise on retinal health and function

The benefits of exercise to human health have long been recognised. However, only in the past decade have researchers started to discover the molecular benefits that exercise confers, especially to the central nervous system (CNS). These discoveries include the magnitude of molecular messages that are communicated from skeletal muscle to the CNS. Despite these advances in understanding, very limited studies have been conducted to decipher the molecular benefits of exercise in retinal health and disease. Here, we review the latest work on the effects of exercise on the retina and discuss its effects on the wider CNS, with a focus on demonstrating the potential applicability and comparative molecular mechanisms that may be occurring in the retina. This review covers the key molecular pathways where exercise exerts its effects: oxidative stress and mitochondrial health; inflammation; protein aggregation; neuronal health; and tissue crosstalk via extracellular vesicles. Further research on the benefits of exercise to the retina and its molecular messages within extracellular vesicles is highly topical in this field.
Global Positioning System Activity Profile in Male Para Footballers With Cerebral Palsy

Objective The aim of the study was to compare training and match loads for cerebral palsy football athletes in a state-level development program to assess the specificity of training for competition in the para sport. Design Time-motion analysis and heart-rate monitoring were applied during 20 training sessions of the Western Australia CP Football 2017 training season and seven competition matches of the 2017 Australian National Championships. The study used an observational study design with between-subjects and within-subjects comparisons of training and match loads. Results There were no significant differences between training and match loads in players’ measurements of speed components and heart rates, with two exceptions: higher match distance covered in very high speed (g = 0.33) and time in heart rate zone 1 (<75% heart rate max, g = 0.45) and less time in heart rate zone 2 (g = 0.42). Conclusions Our results indicate that the athletes were conditioned to perform at similar intensities implemented during training, as demonstrated by the consistency between competition and training data in total distance, relative speed zones, and relative heart rate zones. Coaches should apply this knowledge in the programming of training volume and intensities to obtain peak match fitness and specifically prepare athletes for competition in cerebral palsy football.
DebaryOmics: an integrative –omics study to understand the halophilic behaviour of Debaryomyces hansenii

Debaryomyces hansenii is a non‐conventional yeast considered to be a well‐suited option for a number of different industrial bioprocesses. It exhibits a set of beneficial traits (halotolerant, oleaginous, xerotolerant, inhibitory compounds resistant) which translates to a number of advantages for industrial fermentation setups when compared to traditional hosts. Although D. hansenii has been highly studied during the last three decades, especially in regards to its salt‐tolerant character, the molecular mechanisms underlying this natural tolerance should be further investigated in order to broadly use this yeast in biotechnological processes. In this work, we performed a series of chemostat cultivations in controlled bioreactors where D. hansenii (CBS 767) was grown in the presence of either 1M NaCl or KCl and studied the transcriptomic and (phospho)proteomic profiles. Our results show that sodium and potassium trigger different responses at both expression and regulation of protein activity levels and also complemented previous reports pointing to specific cellular processes as key players in halotolerance, moreover providing novel information about the specific genes involved in each process. The phosphoproteomic analysis, the first of this kind ever reported in D. hansenii, also implicated a novel and yet uncharacterized cation transporter in the response to high sodium concentrations.
Fuzzy PI controlled inverter for grid interactive renewable energy systems

In this study, a fuzzy-PI controlled grid interactive inverter has been designed and implemented. The proportional and integral gains of the PI controller are decided and tuned by the fuzzy logic controller (FLC) according to required operation point of the system. Thus, adaptive nature of the FLC and robust structure of the PI controller are synthesised. Eventually, an adaptive PI controller which can adopt changes because of different operation conditions, grid disturbances and natural effects with fast transient response is obtained. Simulation studies are validated with experimental results. Both simulation and experimental results show that proposed system has fast dynamic response and tracks reference current with a low overshot and short settling time. In addition, the waveform of the inverter output current is sinusoidal and also the current is in same phase and frequency with the line voltage. Furthermore, the total harmonic distortion level of the inverter current meets the international standards. In addition, the proposed inverter system is compared with conventional PI controlled grid interactive inverter with various proportional and integral gains.
Sleep duration and mortality, influence of age, retirement, and occupational group

Previous work has shown that both long and short sleep duration is associated with increased mortality, with lowest risk around 7 hr. This has had widespread impact on views on the optimal sleep duration. However, age, being employed/retired, and blue‐/white‐collar status, may influence the time available for sleep and thus, confound the association. We investigated the role of these factors on the association between sleep duration and mortality. We used employed and retired participants (N = 25,430) from the Swedish National March Cohort and Cox proportional hazards regression to model the shape of the association. We found a significant U‐shaped association in a multivariable model with a hazard ratio (HR) of 1.24 (95% confidence interval [CI] 1.10, 1.39) for <5‐hr sleep duration, and a HR of 1.30 (95% CI 1.12, 1.51) for ≥9‐hr sleep duration, with the lowest HR for 7 hr, but with a span of low HRs from 5 to 8 hr. Unadjusted values showed a pronounced U‐shape. Adjusting for age accounted for most of the attenuation in the multivariable model. Stratification into five age groups showed a significant U‐shape only in those aged >60.3 years at baseline. The shape of the association did not differ between blue‐/white‐collar workers, nor between employed and retired groups. We conclude that the U‐shaped association between sleep duration and mortality is present only in older individuals.
Deficiency in X-linked inhibitor of apoptosis protein promotes susceptibility to microbial triggers of intestinal inflammation

Description Deficiency in XIAP is associated with Paneth cell defects and susceptibility to microbiota-dependent intestinal inflammation. Xiap and gut microbiota sensitivity Genetic variations, especially in Paneth cells, contribute to inflammatory bowel disease (IBD). X-linked inhibitor of apoptosis protein (XIAP) alterations are commonly associated with IBD, but how XIAP deficiency regulates Paneth cells is unclear. Here, Strigli et al. used XIAPKO mice and organoids to determine how XIAP alters IBD. They found that XIAP deficiency (mice and patient samples) made Paneth cells sensitive to cell death induced by microbiota, TNF, and RIPK1/3. Specifically, loss of Paneth cell–derived antimicrobial peptides led to microbiota alterations in the XIAPKO mice, making mice more susceptible to IBD. Last, IBD could be inhibited in XIAPKO mice by administration of antimicrobial peptides, pointing to a potential therapy that could be used for IBD associated with Paneth cell loss. Inflammatory bowel disease (IBD) is characterized by inappropriate immune responses to the microbiota in genetically susceptible hosts, but little is known about the pathways that link individual genetic alterations to microbiota-dependent inflammation. Here, we demonstrated that the loss of X-linked inhibitor of apoptosis protein (XIAP), a gene associated with Mendelian IBD, rendered Paneth cells sensitive to microbiota-, tumor necrosis factor (TNF)–, receptor-interacting protein kinase 1 (RIPK1)–, and RIPK3-dependent cell death. This was associated with deficiency in Paneth cell–derived antimicrobial peptides and alterations in the stratification and composition of the microbiota. Loss of XIAP was not sufficient to elicit intestinal inflammation but provided susceptibility to pathobionts able to promote granulomatous ileitis, which could be prevented by administration of a Paneth cell–derived antimicrobial peptide. These data reveal a pathway critical for host-microbial cross-talk, which is required for intestinal homeostasis and the prevention of inflammation and which is amenable to therapeutic targeting.