Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Dietary Phaffia rhodozyma Astaxanthin on Growth Performance, Carotenoid Analysis, Biochemical and Immune-Physiological Parameters, Intestinal Microbiota, and Disease Resistance in Penaeus monodon

The present study aimed to investigate the effect of dietary astaxanthin (Ast) from Phaffia rhodozyma on growth performance, survival, carotenoid content, the activity of antioxidant and immune-related enzymes, intestinal microbiota comparison, and disease resistance against Vibrio parahaemolyticus in Penaeus monodon. Juveniles (average weight 3.15 ± 0.12 g) were fed with six experimental diets supplemented with 0 (Control), 20.5, 41, 61.5, 82, and 102.5 mg/kg of Ast (defined as diet A–D) in triplicate for 56 days. The results indicated that shrimp fed with Ast supplementation significantly (p < 0.05) improved growth performance compared with the control. Furthermore, significantly (p < 0.05) increased survival and decreased feed conversion ratio (FCR) demonstrated the beneficial effects of dietary Ast on enhancing nutrient utilization and ultimately improving the growth and survival of shrimp. Furthermore, shrimp fed with Ast including diet developed a deeper red color than the control, consistent with the significantly (p < 0.05) increased Ast deposition in the shrimp shell. Hemolymph-immunological parameters [aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (AKP)] and hepatopancreatic antioxidant status [total antioxidant capacity (T-AOC), malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD)] were significantly (p < 0.05) affected by dietary Ast supplementation. Dietary increasing Ast levels significantly (p < 0.05) increased shrimp resistance performance to V. parahaemolyticus according to the LT50 results in the current study, which may be caused by increased total carotenoid contents in shrimp tissues from all the Ast-supplemented treatments. Conversely, intestinal microbiota biodiversity and richness were not affected by dietary Ast. The best performances of growth, antioxidant status, immunological response, and carotenoid deposition were observed in diets E and F among all the Ast-supplemented treatments. Overall, all the data suggested that dietary P. rhodozyma Ast played a critical role in improving growth performance, achieving the desired coloration, increasing carotenoid content, and keeping better health status of shrimp. Based on these positive performances, P. rhodozyma Ast could gain the trust of the consumers as a natural source and provide a potential alternative for synthetic Ast using in the Penaeus monodon culture industry.
Sitting for Too Long, Moving Too Little: Regular Muscle Contractions Can Reduce Muscle Stiffness During Prolonged Periods of Chair-Sitting

In modern Western societies, sedentary behavior has become a growing health concern. There is increasing evidence that prolonged sitting periods can be associated with musculoskeletal disorders. While it is generally recognized that back muscle activity is low during chair-sitting, little is known about the consequences of minor to no muscle activity on muscle stiffness. Muscle stiffness may play an important role in musculoskeletal health. This study investigated the effects of regular muscle contractions on muscle stiffness in a controlled experiment in which participants sat for 4.5 h. Neuromuscular electrical stimulation in the lumbar region of the back was applied to trigger regular muscle contractions. Using stiffness measurements and continuous motion capturing, we found that prolonged sitting periods without regular muscle contractions significantly increased back muscle stiffness. Moreover, we were able to show that regular muscle contractions can prevent those effects. Our results highlight the importance of consistent muscle activity throughout the day and may help explain why prolonged periods of chair-sitting increase the susceptibility to common pathological conditions such as low back pain.
The Influence of Physical Education on Self-Efficacy in Overweight Schoolgirls: A 12-Week Training Program

The purpose of this randomized controlled study was to investigate the impact of a 12-week physical education (PE) program on the self-efficacy of overweight schoolgirls. We randomly assigned 60 overweight schoolgirls (15–17 years) to either an experimental moderate to vigorous aerobic exercise (∼90 min, three times a week) group (n = 30) or a control group (CG) (n = 30) that received non-specific regular PE lessons with activities chosen by the curricular teacher mainly focused on team games and sports skills that aimed to achieve general psycho-physical wellness (∼90 min, three times a week). To assess the starting level of students and significant changes reached, at baseline and after training, a battery of standardized assessment motor tests and a psychometric scale (generalized self-efficacy scale, GES) were administered. At the end of the intervention, the experimental group reported a considerable decrease in body mass index (BMI) and a large improvement in self-efficacy (p < 0.001). No significant changes were found in the CG. The results suggested that the 12-week moderate to a vigorous aerobic exercise program is an effective weight loss intervention and a vehicle to promote a range of outcomes important to the qualitative growth of adolescents. In fact, it could provide a positive and significant impact on the self-efficacy of overweight schoolgirls.
Assessment of the Ecological Status of Rongjiang Estuary (China) Under Human Pressure, Using Biotic Indices Based on Benthic Macroinvertebrates

Rongjiang River, the second largest river system in Guangdong Province, flows through the main urban areas of Jieyang and Shantou cities before reaching the South China Sea. Human activities in the surrounding area pose significant threats to this aquatic ecosystem. The ecological status (ES) of the benthic ecosystem of the Rongjiang River estuary has not yet been conducted using indices based on the macrobenthic fauna, which is important for evaluating environmental health. Here, we used four biotic indices (the AZTI’s Marine Biotic Index (AMBI) and Multivariate AZTI’s Marine Biotic Index (M-AMBI), and taxonomic distinctness indices (average taxonomic distinctness Δ+ and variations in taxonomic distinctness Λ+) to appraise the current ES of benthic communities in Rongjiang estuary. Samples were taken from 11 sampling sites located in six general regions: western aquaculture zones, aquaculture zones, Hanjiang River water channel, Shantou City, Shantou Port, and near the ocean. The benthic ecosystem of this estuary is greatly disturbed: the ES of the aquaculture zones and the sites near the Hanjiang River water channel, Shantou City, and close to the ocean was poorer compared with that of other areas; ES was also poorer in winter than in summer. Generalized linear models revealed that Shannon-Wiener index was negatively correlated with dissolved inorganic nitrogen (p < 0.01), M-AMBI was negatively correlated with temperature and dissolved inorganic nitrogen (p < 0.05 and p < 0.01, respectively), and Λ+ was negatively correlated with pH (p < 0.05). The AMBI, M-AMBI, Δ+, and Λ+ indices were suitable for assessing the ES of the benthic ecosystem in an anthropogenically disturbed estuary.
Personality Factors in Colorectal Cancer: A Systematic Review

Background: The role of personality in cancer incidence and development has been studied for a long time. As colorectal cancer (CRC) is one of the most prevalent cancer types and linked with lifestyle habits, it is important to better understand its psychological correlates, in order to design a more specific prevention and intervention plan. The aim of this systematic review is to analyze all the studies investigating the role of personality in CRC incidence. Methods: All studies on CRC and personality up to November 2020 were scrutinized according to the Cochrane Collaboration and the PRISMA statements. Selected studies were additionally evaluated for the Risk of Bias according to the Newcastle-Ottawa Scale (NOS). Results: Eight studies met the inclusion criteria and were eventually included in this review. Two main constructs have been identified as potential contributors of CRC incidence: emotional regulation (anger) and relational style (egoism). Conclusion: Strong conclusions regarding the influence of personality traits on the incidence of CRC are not possible, because of the small number and the heterogeneity of the selected studies. Further research is needed to understand the complexity of personality and its role in the incidence of CRC and the interaction with other valuable risk factors.
Effect of Bow Camber and Mass Distribution on Violinists' Preferences and Performance

Little is known about how bow mechanical characteristics objectively and quantitatively influence violinists' preferences and performance. Hypothesizing that the bow shape (i.e., camber) and mass distribution modifications would alter both violinists' appreciations of a bow and objective assessments of their performance, we recruited 10 professional violinists to play their own violin using 18 versions of a single bow, modified by combining three cambers and six mass distributions, in random order. A musical phrase, composed for this study, was played legato and spiccato at three octaves and two tempi. Each violinist scored all 18 bows. Then, experts assessed the recorded performances according to criteria inspired by basic musical analysis. Finally, 12 audio-descriptors were calculated on the same note from each trial, to objectivise potential acoustic differences. Statistical analysis (ANOVA) reveals that bow camber impacted the violinists' appreciations (p < 0.05), and that heavier bow tips gave lower scores for spiccato playing (p < 0.05). The expert evaluations reveal that playing with a lighter bow (tip or frog), or with a bow whose camber's maximum curvature is close to the frog, had a positive impact on some violinists' performance (NS to p < 0.001). The “camber-participant” interaction had significant effects on the violinists' appreciations (p < 0.01 to p < 0.001), on the expert's evaluation and on almost all the audio-descriptors (NS to p < 0.001). While trends were identified, multiple camber-participant interactions suggest that bow makers should provide a variety of cambers to satisfy different violinists.
A Novel Method for Estimating Spatial Distribution of Forest Above-Ground Biomass Based on Multispectral Fusion Data and Ensemble Learning Algorithm

Optical remote sensing technology has been widely used in forest resources inventory. Due to the influence of satellite orbits, sensor parameters, sensor errors, and atmospheric effects, there are great differences in vegetation spectral information captured by different satellite sensor images. Spectral fusion technology can couple the advantages of different multispectral sensor images to produce new multispectral data with high spatial and spectral resolution, it has great potential for improving the spectral sensitivity of forest vegetation and alleviating the spectral saturation. However, how to quickly and effectively select the multi-spectral fusion data suitable for forest above-ground biomass (AGB) estimation is a very critical issue. This study proposes a scheme (RF-S) to comprehensively evaluate multispectral fused images and develop the appropriate model for forest AGB estimation, on the basis of random forest (RF) and the stacking ensemble algorithm. First, four classic fusion methods are used to fuse the preprocessed GaoFen-2 (GF-2) multispectral image with Sentinel-2 image to generate 12 fused Sentinel-like images. Secondly, we apply a comprehensive evaluation method to quickly select the optimal fused image for the follow-up research. Subsequently, two feature combination optimization methods are used to select feature variables from the three feature sets. Finally, the stacking ensemble algorithm based on model dynamic integration and hyperparameter automatic optimization, as well as some classic machine learners, are used to construct the forest AGB estimation model. The results show that the fused image NND_B3 (based on nearest neighbor diffusion pan sharpening method and Band3_Red) selected by the evaluation method proposed in this study has the best performance in AGB estimation. Using the stacking ensemble method and NND_B3 image, we get the highest estimation accuracy, with the adjusted R2 and relative root mean square error (RMSEr) of 0.6306 and 15.53%, respectively. The AGB estimation RMSEr of NND_B3 is 19.95% and 24.90% lower than those of GF-2 and Sentinel-2, respectively. We also found that the multi-window texture factor has better performance in the area with low AGB, and it can suppress the overestimation significantly. The AGB spatial distribution estimated using the NND_B3 image matches the field observations well, indicating that the multispectral fusion image combined with the Stacking algorithm can increase the accuracy and saturation of the AGB estimates.
Analytic response relativistic coupled-cluster theory: the first application to indium isotope shifts

With increasing demand for accurate calculation of isotope shifts of atomic systems for fundamental and nuclear structure research, an analytic energy derivative approach is presented in the relativistic coupled-cluster (CC) theory framework to determine the atomic field shift and mass shift (MS) factors. This approach allows the determination of expectation values of atomic operators, overcoming fundamental problems that are present in existing atomic physics methods, i.e. it satisfies the Hellmann–Feynman theorem, does not involve any non-terminating series, and is free from choice of any perturbative parameter. As a proof of concept, the developed analytic response relativistic CC theory has been applied to determine MS and field shift factors for different atomic states of indium. High-precision isotope-shift measurements of 104 − 127 In were performed in the 246.8 nm (5p 2P3/2 → 9s 2S1/2) and 246.0 nm (5p 2P1/2 → 8s 2S1/2) transitions to test our theoretical results. An excellent agreement between the theoretical and measured values is found, which is known to be challenging in multi-electron atoms. The calculated atomic factors allowed an accurate determination of the nuclear charge radii of the ground and isomeric states of the 104 − 127 In isotopes, providing an isotone-independent comparison of the absolute charge radii.
3D Interaction Homology: Computational Titration of Aspartic Acid, Glutamic Acid and Histidine Can Create pH-Tunable Hydropathic Environment Maps

Aspartic acid, glutamic acid and histidine are ionizable residues occupying various protein environments and perform many different functions in structures. Their roles are tied to their acid/base equilibria, solvent exposure, and backbone conformations. We propose that the number of unique environments for ASP, GLU and HIS is quite limited. We generated maps of these residue's environments using a hydropathic scoring function to record the type and magnitude of interactions for each residue in a 2703-protein structural dataset. These maps are backbone-dependent and suggest the existence of new structural motifs for each residue type. Additionally, we developed an algorithm for tuning these maps to any pH, a potentially useful element for protein design and structure building. Here, we elucidate the complex interplay between secondary structure, relative solvent accessibility, and residue ionization states: the degree of protonation for ionizable residues increases with solvent accessibility, which in turn is notably dependent on backbone structure.
Strategies for Developing Transition Metal Phosphides in Electrochemical Water Splitting

Electrochemical water splitting involving hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is a greatly promising technology to generate sustainable and renewable energy resources, which relies on the exploration regarding the design of electrocatalysts with high efficiency, high stability, and low cost. Transition metal phosphides (TMPs), as nonprecious metallic electrocatalysts, have been extensively investigated and proved to be high-efficient electrocatalysts in both HER and OER. In this minireview, a general overview of recent progress in developing high-performance TMP electrocatalysts for electrochemical water splitting has been presented. Design strategies including composition engineering by element doping, hybridization, and tuning the molar ratio, structure engineering by porous structures, nanoarray structures, and amorphous structures, and surface/interface engineering by tuning surface wetting states, facet control, and novel substrate are summarized. Key scientific problems and prospective research directions are also briefly discussed.
Choosing Death Over Survival: A Need to Identify Evolutionary Mechanisms Underlying Human Suicide

The act of killing self contradicts the central purpose of human evolution, that is, survival and propagation of one’s genetic material. Yet, it continues to be one of the leading causes of human death. A handful of theories in the realm of evolutionary psychology have attempted to explain human suicide. The current article analyses the major components of certain prominent viewpoints, namely, Inclusive fitness, Bargaining model, Pain-Brain model, Psychological aposematism, and few other perspectives. The article argues that relatively more weightage has been given to understanding ultimate (the “why”) rather than proximate (the “how”) functionality of suicidal acts. Evolutionary theorists have consistently pointed out that to comprehensively understand a trait or behavior, one needs to delineate not only how it supports survival but also the evolution of the mechanisms underlying the trait or behavior. Existing theories on suicide have primarily focused on its fitness benefits on surviving kin instead of providing evolutionary explanations of the more complex mechanisms leading up to such self-destructive motivations. Thus, the current paper attempts to highlight this gap in theorizing while suggesting probable proximate explanations of suicide which stresses the need to diffuse attention paid to fitness consequences of the act alone. We speculate that such explorations are needed in order to build a robust and comprehensive evolutionary theory of human suicide.
Drug Resistant Tuberculosis in the Northern Region of Zambia: A Retrospective Study

Background Zambia like many countries in sub-Saharan Africa is affected with drug resistant tuberculosis. However, the drug resistant tuberculosis situation over the years has not been described in various regions of the country. Consequently, this study aims to determine the drug resistant tuberculosis burden in northern regions of Zambia over a four-year period based on data generated from a Regional Tuberculosis Reference Laboratory. Method Two hundred and thirty two (232) Tuberculosis Drug Susceptibility Testing results over a four-year period (2016-2019) were reviewed. Data was collected from tuberculosis registers and patient request forms and entered into a pre-tested standardized checklist and later entered in Excel Computer software. Double blinded checking was done by two independent data clerks to minimize duplication of cases. Cleaned data was then imported in R programme for analysis. Bivariant and descriptive statistics were performed and reported. Results Of 232 Drug Susceptibility Testing results, 90.9% were drug resistant TB while 9% were drug susceptible. Fifty three percent (53%) of these were multi-drug resistant Tuberculosis and 32% were confirmed as Rifampicin Mono-resistance. Only 1.7% of the Multi-drug resistant Tuberculosis patients were Pre-extensively drug-resistant Tuberculosis. Copperbelt province had the largest proportion (46.0%) of multi-drug resistant tuberculosis patients followed by Luapula (8.1%) and North-Western (4.7%) provinces. In new and previously treated patients, the proportion of Multi-drug resistant tuberculosis was 71.8% and 28.7% respectively. History of previous anti-tuberculosis treatment and treatment failure were associated with multi-drug resistance TB. Conclusion and Recommendation This study has shown a small increase in the proportions of drug resistant tuberculosis cases over the four years under review with high rates being recorded on the Copperbelt Province. Previous treatment to first line TB treatment and treatment failure were associated with development of Multi-drug resistance. We therefore recommend strengthened routine laboratory surveillance and improved case management of multi-drug resistant tuberculosis patients in the region.
(-)-T-Cadinol—a Sesquiterpene Isolated From Casearia sylvestris (Salicaceae)—Displayed In Vitro Activity and Causes Hyperpolarization of the Membrane Potential of Trypanosoma cruzi

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and affects 6–8 million people worldwide, mainly from developing countries. The treatment is limited to two approved nitro-derivatives, nifurtimox and benznidazole, with several side effects and reduced efficacy. Casearia sylvestris has been used in folk medicine as an antiseptic and cicatrizing in skin diseases. In the present work, the hexane phase from the MeOH extract from the leaves of Casearia sylvestris afforded a fraction composed by the sesquiterpene T-cadinol, which was chemically characterized by NMR and HRMS. The activity of T-cadinol was evaluated against T. cruzi, and IC50 values of 18 (trypomastigotes) and 15 (amastigotes) µM were established. The relation between the mammalian toxicity and the antiparasitic activity resulted in a selectivity index >12. Based on this promising activity, the mechanism of action was investigated by different approaches using fluorescent-based techniques such as plasma membrane permeability, plasma membrane electric potential, mitochondrial membrane electric potential, reactive oxygen species, and the intracellular calcium (Ca2+) levels. The obtained results demonstrated that T-cadinol affected neither the parasite plasma membrane nor the electric potential of the membrane. Nevertheless, this compound induced a mitochondrial impairment, resulting in a hyperpolarization of the membrane potential, with decreased levels of reactive oxygen species. No alterations in Ca2+ levels were observed, suggesting that T-cadinol may affect the single mitochondria of the parasite. This is the first report about the occurrence of T-cadinol in C. sylvestris, and our data suggest this sesquiterpene as an interesting hit compound for future optimizations in drug discovery studies for Chagas disease.
How Servant Leadership Sparks Feedback-Seeking Behavior: A Moderated Mediation Model

Drawing upon social information processing theory, we propose that moqi with supervisors mediates the relationship between servant leadership and follower feedback-seeking behavior. Subordinates’ traditionality plays a moderating role in this process. A total of 440 Chinese working adults responded to the two-wave questionnaire survey in paper and pencil forms. Correlation analyses, mediation analysis, and moderated mediation analysis was performed through R and SPSS PROCESS Macro. The results revealed that servant leadership positively correlates with followers’ feedback-seeking behavior via moqi with supervisors. Moreover, these indirect effects of servant leadership were moderated by traditionality, such that servant leadership had weaker relations with feedback-seeking behavior when traditionality was higher (vs. lower). Theoretical contributions and practical implications, limitations and suggestions for further study were discussed.