Miracle-dz/UniFAR-FTUs huggingface dataset:
Association of specific microorganisms with endodontic signs and symptoms. A comparative study

Aim: The study aimed to evaluate the association of root canal microorganisms red complex and E. facealis with endodontic clinical signs and symptoms using polymerase chain reaction. Materials and Methods: Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers. Results: The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and E. faecalis bacteria observed both in primary infection and secondary infection (P < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association (P < 0.05). Conclusion: Prevalence of red complex bacteria and E. faecalis suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.
Femtosecond laser-assisted anterior capsulotomy for capsule phimosis

The centripetal constriction and fibrosis of the capsulorhexis following cataract surgery are known as anterior capsule phimosis. A 44-year-old myopic female who underwent uneventful phacoemulsification with in-the-bag intraocular lens implantation in the right eye presented 2 months later with cloudy vision due to significant capsular phimosis. Her best-corrected visual acuity was 20/32 in the right eye. She underwent femtosecond laser-assisted capsulotomy using a power of 10 μJ. Femtosecond laser is a more precise and predictable method of treating anterior capsule phimosis as compared to the existing treatment modalities, such as neodymium: YAG capsular opening.
A Case-control Study Comparing and Correlating iNOS Expression among Various Clinicopathological Variants of Oral Leukoplakia and Oral Squamous Cell Carcinoma: A Immunohistochemistry Study

Background and Objective: The role of inducible nitric oxide synthase (iNOS) has been implicated in various pathological processes including oral carcinoma. Oral premalignancy being its precursor lesion is also expected to show similar pattern. This study attempts to appraise the iNOS expression in various clinicopathological stages and grades of oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: A case-control study design was adopted for this study with a total sample of 90 subjects, distributed equally into the three study groups, namely controls, OL, and OSCC. Clinical staging and histopathological grading for both the case groups were performed. Representative tissue samples from all groups were obtained and studied for iNOS expression using immunohistochemistry (IHC). Data were presented in mean and percentages accordingly. Inferential analysis was performed using Kruskal–Wallis test, Mann–Whitney test, and Spearman rank correlation test. Results: Significant (P < 0.001) difference was observed among the groups, where 83.3% of OSCC and 73.3% of OL epithelial cells showed iNOS expression. The normal cells did not show up any expression. The expression was found to rise with the progressing clinical stages of OL (P < 0.05) and OSCC (P < 0.01). Similar pattern was observed with respect to advancing dysplasia in OL (P < 0.01) and cell differentiation in OSCC (P < 0.01). Significant positive correlation was found in clinicopathological categories of OL and OSCC. Considering the risk assessment, iNOS staining was found to be significantly raised in advanced cases of OSCC (P < 0.01) and high-risk cases of OL (P < 0.01). Conclusion: Increased expression of iNOS can be an early diagnostic marker in OL and as prognostic marker in OSCC.
Metabolic regulation in bacterial continuous cultures: II

The transient behavior of a continuous culture of Klebsiella pneumoniae with mixed feed of glucose and xylose arising from step‐up and step‐down in dilution rates and from a feed‐switching experiment is presented. he organism gradually switches from simultaneous utilization of the substrates at low growth rates to preferred utilization of the faster substrate (i.e, supporting a higher growth rate) at high dilution rates. The metabolic lags following a step increase in dilution rate and a significant accumulation of the slower substrate during the transient period result from the effects of metabolic regulation. The cybernetic modeling approach that successfully described the foregoing situations with single‐substrate feeds is employed to describe mixed substrate behavior. The parameters in the mixed‐substrate (glucose and xylose) model are the same as those in the single‐substrate models with the singular exception of the rate constant for the xylose growth enzyme synthesis. The reason for this discrepancy is discussed in detail. It appears that the constitutive rate of enzyme synthesis for growth on a given substrate may be related to the past history of the organism in regard to whether or not the organism has been exposed to the particular substrate. Thus, the results further demonstrate the ability of the framework to effectively describe metabolic regulation in batch, fedbatch, and continuous microbial cultures.
Influence of Different Zirconia Coping Designs on the Fracture Resistance of All-Ceramic Crowns: An In Vitro Study

Background and Aim: Increased demand for metal-free restorations in posterior areas has increased the focus on zirconia restorations because of its advantages in excellent mechanical properties, patient comfort, and acceptance. Although all-ceramic crowns have better biocompatibility and esthetic properties, there are some clinical reasons for the fracture of the veneer or the core materials in the posterior region. This study aims at comparing the influence of zirconia coping designs on the fracture resistance of all-ceramic crowns. Materials and Methods: A total of 32 zirconia copings (n = 8 for each group) were designed and fabricated based on the marginal collar height of zirconia coping. The groups were the following: Group 1: 0.0 mm zirconia coping; Group 2: 0.5 mm collar; Group 3: 1.0 mm collar; and Group 4: 1.5 mm collar. All these zirconia copings were veneered with porcelain. Master die with cemented ceramic-layered zirconia copings was tested using Universal testing machine. Data obtained were statistically evaluated by one-way analysis of variance and post hoc test. Results: There was statistically significant difference between the groups with P < 0.001. Group 4 has the highest strength (3318.89 ± 395.67) followed by Group 3 (2910.0 ± 219.22), then Group 1 (2320.02 ± 547.36) that is the control group and the least strength with Group 2 (2286.59 ± 496.66). Conclusion: On the basis of the results obtained from this study, it can be concluded that, the more the height of the zirconia collar, the higher the fracture strength of the all-ceramic crowns. Thus ceramic-layered zirconia coping design with 1.5 mm marginal collar height is recommended for functional long life, at least in the posterior higher load-bearing areas with lesser esthetic demands.
Protocol-Based Early Decompressive Craniectomy in a Resource-Constrained Environment: A Tertiary Care Hospital Experience

Objectives: Decompressive craniectomy (DC) is an emergency life-saving procedure used to treat refractory intracranial hypertension (RICH). The authors aim to analyze their experience with protocol-based early DC (<24 h) in RICH cases diagnosed based on clinical and radiological evidence, without preoperative intracranial pressure monitoring done over 10 years. Materials and Methods: This is a retrospective, observational study which includes 58 consecutive patients who underwent protocol-based early DC by the senior author at a single institution between 2007 and 2017. Background variables and outcome in the form of Glasgow Outcome Score-Extended (GOS-E) at 6 months and 1 year were analyzed. Results: Fourteen patients had traumatic brain injury (TBI), 17 had intracranial hemorrhage (ICH), 14 had malignant cerebral infarcts (MCI), and the reminder 13 patients had other causes. At 6 months, the mortality rate was 22.4%. Good recovery, moderate disability, and severe disability were seen in 13.8%, 17.2%, and 43.1% of patients, respectively. Two patients were in vegetative state. The cutoff for favorable/unfavorable outcome was defined as GOS-E 4–8/1–3. By this application, 63.8% of patients had favorable outcome at 6 months. The favorable outcome in patients of TBI, ICH, and MCI was 57.1%, 58.8%, and 85.7%, respectively. Conclusions: DC helps in obtaining a favorable outcome in selected patients with a defined pathology. The diagnosis of RICH based on clinical and radiological parameters, and protocol-based early DC, is reasonably justified as the way forward for resource-constrained environments. The risk of vegetative state is small.
An Immunohistochemical Study Showing Ki-67 as an Analytical Marker in Oral Malignant and Premalignant Lesions

Introduction: Ki-67 is a nuclear protein. It is a proliferation marker that has an essential function in tumorigenesis due to its positive connection with tumor expansion. Aim: The aim of this study was to evaluate the articulation of Ki‑67 as prognostic marker in various grades of oral epithelial dysplasia (OED) and in oral squamous cell carcinoma (OSCC). Materials and Methods: A total of 100 histologically affirmed samples of normal oral mucosa (NOM), OED, and OSCC were divided into three groups—Group I (10 samples of normal oral mucosa), Group II (45 samples of OED), Group III (45 samples of OSCC). Routine hematoxylin and eosin and immunohistochemical staining with Ki-67 monoclonal antibody were carried out in all the samples. Results: Within Group I, articulation of Ki-67 was constrained to the basal layers. In Group II, cells showing positive expression of Ki-67 were available in the basal, suprabasal, and spinous layers. Cells showing positive expression of Ki-67 among well-differentiated OSCC were presented mainly in the periphery of the tumor nests; in moderately differentiated OSCC, cells were located in both peripheral and part of a center of the tumor nests; and in most cases of poorly differentiated OSCC, cells were diffused. Statistically significant difference in positive expression of Ki-67 was appreciated between three groups. Conclusion: Ki-67 antigen may perhaps be used as a marker for the histological reviewing of OED and OSCC. With the increase in the severity of OED, cells showing positive expression of Ki-67 also increased.
Use of ITS and ISSR markers to identify cultivated strains for {\sl Lentinula edodes}

16 samples were selected including 12 cultivated strains with similar or confused names for Lentinula edodes, 3 strains for Lentinus tigrinus, and one strain for Lentinus lepideus in this study. These samples were firstly analyzed at the level of species by ITS technique, and then were analyzed by ISSR technique. The sizes of the fragments from 3 species amplified by ITS technique were different, which can be used to identify strains from different species. Two fragments owned to Lentinula edodes strains were obtained and 5 pairs of strains for Lentinula edodes showed the same band patterns each other while strains from different species had different band patterns by ISSR analysis. The results were proved by RAPD analysis. In a conclusion, ITS and ISSR techniques can be used to identify cultivated strains for Lentinula edodes.
The relationship between DMFT index and cognitive impairment: A descriptive cross-sectional study

Background and Aims: Cognitive disorders are characterized by major impairments in functions such as memory, judgment, language and attention. One of the tests used to assess these disorders is called the Montreal Cognitive Assessment Test, which is one of the most recent tests known in this field. DMFT index is the most important dental index for evaluation of decayed, missing and filled teeth. Since alteration or damage to the sensory function of teeth can cause changes in chewing function and different levels of cognitive disorders. The objective of this study was to determine the relationship of DMFT index with cognitive disorders in people aged 25-55 in Rafsanjan in 2019. Materials and Methods: This cross-sectional study was performed on 350 patients chosen by simple random sampling within age range of 25-55 years, referred to Rafsanjan Dental School in 2019. Patients' demographic information was recorded and each was given a consent form and also Montreal Cognitive Assessment Test. Dental examination regarding decayed, missing and filled teeth was then carried out by a dentist and The DMFT index was recorded. Data were then analyzed by SPSS version 21; P value less than 0.05 was considered as significant level. Results: Data showed that the MOCA index had a negative significant relationship with DMFT (P = 0.001) and age (P = 0.001) and had a significant positive relationship with education (P = 0.001), but it had no significant relationship with gender (P = 0.853), time of last dental appointment (P = 0.231) and place of residence (P = 0.428). The effect of age (P = 0.67) and gender (P = 0.525) on the relationship of DMFT and MOCA was not significant. But the effect of education (P = 0.001), place of residence (P = 0.0003) and time of last dental appointment (P = 0.002) on it was significant. Conclusion: The results of this study showed that the relationship between DMFT index and cognitive disorders was significant and the higher the score of this index, the more severe cognitive disorders.
Estimation of Red-complex Bacteria in Diode Laser Treated Chronic Periodontitis Patients: A Clinical and Microbiological Study

Context: Laser has been widely accepted as a substitute to traditional periodontal treatment. Only a finite number of studies are available based on the use of diode laser as a supplement to scaling and root planing (SRP) in the reduction of red-complex bacteria. Aim: This split-mouth study was aimed to determine the clinical and microbiological effects of diode laser as a supplement to SRP. Materials and Methods: For this split-mouth study, systemically healthy 34 patients with chronic periodontitis were selected. In the test quadrant, SRP + laser therapy was carried out, whereas in control quadrants, SRP alone was performed. Clinical and microbiological data were acquired at baseline and 3 months postoperative, and statistical analysis was carried out on the findings. Results: The results showed that both the treatment modalities were impelling. Considerable reduction in the mean probing depth and a notable improvement in the attachment level were observed in both groups in comparison to baseline, with a statistically significant reduction in the laser group. Microbiological analysis results showed more reduction in red-complex bacteria in the laser group compared to the SRP group, but they were statistically insignificant. Conclusion: Within the limitation of this study, it is recommended that both the SRP and SRP + laser are effective in chronic periodontitis management, but using laser with SRP has propitious results. Thus, in the forthcoming years, clinical experiments with a greater sample size may be chosen to further analyze the fringe benefits of laser as a supplement to SRP.
Magnetic Resonance Imaging Findings of Idiopathic Normal Pressure Hydrocephalus and Cognitive Function Before and After Ventriculoatrial Shunt

Background: The idiopathic normal pressure hydrocephalus (iNPH) is characterized by the triad of gait impairment, incontinence, and dementia. Cases that do not comply with the diagnostic criteria of ventriculomegaly have increased. It has led to the questions about the current criteria of guidelines. As the number of patients with dementia increases with aging, iNPH is importantly placed as a treatable dementia. The purpose of this study was to verify the validity of radiological diagnostic criteria of ventriculomegaly in iNPH. Materials and Methods: A board-certified neuroradiologist retrospectively examined 80 patients with definite iNPH about magnetic resonance imaging (MRI) findings of Evans index (EI) and disproportionately enlarged subarachnoid space hydrocephalus (DESH). The score of mini-mental state examination (MMSE) was measured to represent the cognitive function. The presurgical score of MMSE (pre-MMSE) and postsurgical best score of MMSE (best-MMSE) were compared statistically between patients dichotomized by either EI >0.3 or DESH. Results: The pre-MMSE was not different regardless of dichotomization by EI >0.3 or DESH. The MMSE score (median) increased significantly (P < 0.0001) by shunt from 20.0 to 26.0 in patients with EI >0.3 and from 21.5 to 25.5 with EI ≤0.3. No difference in the best-MMSE was observed between EI >0.3 and EI ≤0.3. The MMSE score increased significantly (P < 0.0001) by shunt from 21 to 27.5 with DESH and from 20 to 24.5 with non-DESH. Regardless of fulfilling or notfulfilling Japanese radiological diagnostic criteria (combination of EI >0.3 and DESH), cognitive function was significantly (P < 0.0001) improved to the same level. Only 24 cases (30%) fulfilled Japanese radiological diagnostic criteria. Conclusion: Cognitive function of iNPH patients was significantly improved by shunt regardless of MRI-findings. Radiological diagnostic criteria of iNPH may need careful reconsideration.
Phenology of a bee (Hymenoptera: Apoidea) community over a 10 year period in south‐eastern Australia

Bee responses to anthropogenic disturbances have received much recent attention in scientific literature. These studies typically involve 1–3 years of sampling along spatial gradients of disturbance, though occasionally greater temporal replication and/or longer time periods are used at the expense of spatial replication. We surveyed bees using a blue vane trap during spring, summer and autumn from 2008 to 2017 at one location in Canberra, Australia. To the best of our knowledge, this is the longest near‐continuous record of bee activity in the southern hemisphere. We use these data to describe the temporal dynamics of the bee community, which was characterised by high annual variability in overall abundance, richness and composition and a negative correlation between spring rainfall and bee activity. The phenologies of abundant species are also described. Our findings relate only to our study site but are similar to findings from other long‐term studies conducted in the northern hemisphere, which collectively present a picture of high natural variability in bee communities that must be considered when interpreting findings of bee responses to anthropogenic disturbances.
Impact of Grid-Forming Control and the Available Energy in DC Links on the System Frequency after a System Split

The change in the generation structure towards power plants interfaced to the grid via power-electronics (PE) leads to a reduction of the available rotating masses and moreover, to a higher utilization of the transmission grid. This increases the risk of cascading outages and possible system splits. In addition, the structural change results in possibly higher power imbalances and therefore higher initial frequency gradients after a system split, which makes the controllability of such phenomena more challenging. In order to be able to manage system splits, to stabilize the resulting sub-grids and to avoid blackouts in the future, alternative solutions for the provision of inertia are necessary. One possibility is to provide synthetic inertia by using a grid-forming control for the PE-interfaced power plants. It is possible to operate the inverter at a lower DC link voltage. This way, a fraction of the energy stored in the DC link can be used. The simulation results show that the available energy within the DC link can reduce the initial frequency gradient after a disturbance while keeping the inverter operational. In terms of the time required to initiate countermeasures after a system split, additional energy storage systems such as supercapacitors or battery storage systems are needed, especially in the case of an inverter-dominated grid.
CRISPR-Cas9 DNA Base-Editing and Prime-Editing

Many genetic diseases and undesirable traits are due to base-pair alterations in genomic DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based technologies, can directly install point-mutations in cellular DNA without inducing a double-strand DNA break (DSB). Two classes of DNA base-editors have been described thus far, cytosine base-editors (CBEs) and adenine base-editors (ABEs). Recently, prime-editing (PE) has further expanded the CRISPR-base-edit toolkit to all twelve possible transition and transversion mutations, as well as small insertion or deletion mutations. Safe and efficient delivery of editing systems to target cells is one of the most paramount and challenging components for the therapeutic success of BEs. Due to its broad tropism, well-studied serotypes, and reduced immunogenicity, adeno-associated vector (AAV) has emerged as the leading platform for viral delivery of genome editing agents, including DNA-base-editors. In this review, we describe the development of various base-editors, assess their technical advantages and limitations, and discuss their therapeutic potential to treat debilitating human diseases.
Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Rapid development of versatile layer-by-layer technology has resulted in important breakthroughs in the understanding of the nature of molecular interactions in multilayer assemblies made of polyelectrolytes. Nowadays, polyelectrolyte multilayers (PEM) are considered to be non-equilibrium and highly dynamic structures. High interest in biomedical applications of PEMs has attracted attention to PEMs made of biopolymers. Recent studies suggest that biopolymer dynamics determines the fate and the properties of such PEMs; however, deciphering, predicting and controlling the dynamics of polymers remains a challenge. This review brings together the up-to-date knowledge of the role of molecular dynamics in multilayers assembled from biopolymers. We discuss how molecular dynamics determines the properties of these PEMs from the nano to the macro scale, focusing on its role in PEM formation and non-enzymatic degradation. We summarize the factors allowing the control of molecular dynamics within PEMs, and therefore to tailor polymer multilayers on demand.
Sensors for rate‐adaptive pacing: How they work, strengths, and limitations

Chronotropic incompetence is the inability of the sinus node to increase heart rate commensurate with increased metabolic demand. Cardiac pacing alone may be insufficient to address exercise intolerance, fatigue, dyspnea on exertion, and other symptoms of chronotropic incompetence. Rate‐responsive (adaptive) pacing employs sensors to detect physical or physiological indices and mimic the response of the normal sinus node. This review describes the development, strengths, and limitations of a variety of sensors that have been employed to address chronotropic incompetence. A mini‐tutorial on programming rate‐adaptive parameters is included along with emphasis that patients' lifestyles and underlying medical conditions require careful consideration. In addition, special sensor applications used to respond prophylactically to physiologic signals are detailed and an in‐depth discussion of sensors as a potential aid in heart failure management is provided.