Miracle-dz/UniFAR-FTUs huggingface dataset:
Direct Interaction between Gingival Fibroblasts and Lymphoid Cells Induces Inflammatory Cytokine mRNA Expression in Gingival Fibroblasts

In inflamed periodontal lesions, dense infiltration of lymphocytes is usually observed in the extravascular periodontal connective tissue, adjacent to gingival fibroblasts. Our previous study revealed that activated lymphocytes can adhesively interact with gingival fibroblasts in vitro. In the present study, we investigated whether gingival fibroblasts are activated through direct interaction with lymphoid cells by monitoring the expression of inflammatory cytokine mRNA in human gingival fibroblasts (HGF). Co-culture with various human lymphoid cells in vitro resulted in a marked increase in the expression of IL-la, IL-1β, and IL-6 mRNA by the HGF. In addition, expression of the mRNA of the IL-1β-converting enzyme (ICE), which is essential to produce the mature form of IL-1β, was constitutively observed in the HGF, suggesting that mature IL-lp is produced by these cells. When HGF were cultured with the culture supernatant of the lymphoid cells, the increase in the inflammatory cytokine mRNA expression was not observed. Similarly, when HGF and lymphoid cells were cultured in the same well but separated by a membrane which prevented direct contact between the cells, no increase in inflammatory cytokine mRNA expression was observed. These results strongly indicate that direct interaction between these heterotypic cell types transduces activation signals into HGF that induce an increase in inflammatory cytokine mRNA expression. Furthermore, IL-1β mRNA expression in the HGF was synergistically increased when HGF directly interacted with lymphoid cells in the presence of exogeneous IL-1β. The present study demonstrates that direct interaction between HGF and lymphoid cells stimulates HGF to increase inflammatory cytokine mRNA expression, and raises the possibility that heterotypic cell-cell interaction may facilitate local inflammatory reactions.
Investigation on friction performance of graphene

Graphene is a newly emerging material in many different fields because of its special physical structure. This literature focuses on the influence of layer numbers on friction characteristics. The sample is deposited on a SiO2 substrate. Optical microscope is used to precisely position the specimen while AFM and Raman spectroscopy are suitable tools to determine the layer number of the sheet material. It is found that both the friction force and friction coefficient are reduced with the increase of layer numbers. Moreover, it is also investigated that friction force increases with the velocity.
Magnetic resonance imaging of pathological processes in rodent models of amyotrophic lateral sclerosis

Abstract Non-human models of neurodegenerative diseases have potential for the identification of key pathways in pathogenesis and for the more rapid assessment of therapeutic candidates. While there are legitimate concerns about the physiological differences between the rodent and human motor systems, mice expressing the ‘G93A’ superoxide dismutase-1 gene mutation are a predictable and robustly-characterized model for amyotrophic lateral sclerosis (ALS). This model has provided evidence for an important role of inflammatory processes during the pre-clinical phase, a stage currently inaccessible for human study in what is largely a sporadic disease. While magnetic resonance imaging is now an established and leading modality for the identification of ALS biomarkers in humans, it can also be increasingly applied to rodent models to probe structural, functional and biochemical changes throughout the course of the disease, with additional potential to generate surrogate markers for the efficacy of therapeutic interventions. Targeted MRI contrast agents, through tagging of various cell types and even individual molecules, will deliver an era of in vivo molecular neuroimaging, with greater specificity for the most relevant pathological processes. These are potentially important steps towards the ultimate goal of human therapeutic translation.
Los rasgos temáticos y de estilo propios del periodismo de servicio en las revistas femeninas de alta gama

Esta tesis analiza la relacion e influencia mutua entre cinco revistas femeninas de alta gama (Elle, Marie Claire, Telva, Vogue y Woman) y la corriente periodistica conocida como periodismo de servicio. Se ha estudiado que tipo de informacion ofrecen a su audiencia las revistas femeninas de alta gama para verificar si dicha informacion se corresponde con los rasgos distintivos (desde el punto de vista tematico y desde el punto de vista de estilo y formal) del periodismo de servicio. Las metodologias empleadas han sido: la revision de fuentes documentales y bibliografia para la construccion del marco teorico, las entrevistas estructuradas para completar este estudio de la cuestion teorico -pero tambien para interpretar los datos del estudio empirico- y el analisis de contenido para el diseno del mencionado estudio analitico. Con la informacion obtenida de este planteamiento metodologico se ha verificado la hipotesis principal y tambien las especificas: el hecho de que el contenido predominante en las revistas analizadas sea el estilo de vida indica que es recomendable revisar la clasificacion de las revistas femeninas de alta gama y situarlas en el mismo segmento que las masculinas (consideradas por la OJD como revistas de estilo de vida). Las publicaciones estudiadas muestran a los personajes que aparecen en sus paginas recurriendo a la personificacion. Dato que pone de manifiesto una nueva diferencia entre las revistas femeninas de alta gama con las revistas del corazon en las que, tema y personaje, se fusionan. La indagacion bibliografica para la construccion del marco teorico evidencio que la entrada del discurso del ambito de lo privado en medios caracteristicamente asociados al discurso de lo publico (los diarios de los siglos XVIII y XIX) fue el origen de la prensa femenin
A SOFTWARE SIMULATION TOOL DEDICATED TO THE DESIGN AND EVALUATION OF X-RAY BACKSCATTER SYSTEMS

Backscatter X-ray technique is particularly convenient for the inspection of single-sided access objects, in order to detect subsurface defects or control subsurface layers thickness. This well known technique can benefit today from recent developments in detectors technology. Backscatter technique requires many parameters: relative geometry of source, detector and object, and associated collimation. In order to help the design of backscatter systems, we have developed a software simulation tool, able to predict the backscatter signals for given systems and objects. Theoretically, such a simulation should be performed based on Monte Carlo code to manage multiple scatter interactions. We propose a version based on analytical computation, allowing fast simulation, and a modified one using partially a Monte Carlo code, if more accuracy is required. Experimental measurements are compared to simulation. The proposed tool can also help the interpretation and analysis of backscatter signal for complex objects.
Dual requirement of Walker carcinosarcoma 256 in vitro for asparagine and glutamine.

Reports Accumulation of Reduced Pyridine Nucle6tides by Illuminated Grana: A. San Pietro and H. M. Lang Adenoviruses: Group Name Proposed for New Respiratory-Tract Viruses: J. F. Enders et i. Encoding Nonintegers in a General p-adic Number System: D. K. Cheng Some Serotoninlike Activities of Lysergic Acid Diethylamide: E. Shaw and D. W. Woolley Gamma-Ray Activity of Contemporary Man: C. E. Miller and L. D. Marinelli Mitochondrial Self-Duplication Observed in vitro: H. A. Leon and S. F. "CoC ok. Dual Requirement of Walker Carcinosarcoma 256 in vitro for Asparagine and Glutamine: R. E. Neuman and T. A. McCoy Induced Biosynthesis of Uricase in Yeast: A. H. Roush and A. J. Domnas On Space Group and Molecular Orientation of Azulene: Y. Takeuchi and R. Pepinsky International Relations in Science and Problems of Visas: J. J. Nassau. Neoplastic Changes Developing in Epithelial Cell Lines Derived from Normal Persons: A. E. Moore, C. M. Southam, S. S. Sternberg X-ray Microscopy of Veins of the Skull: C. G. Hewes et al.
. edu . au 3 D local derivative pattern for hyperspectral face recognition

Traditional 2D face recognition has been studied for many years and has achieved huge success. Nonetheless, there is high demand to explore unrevealed information other than structure and texture in spatial domain in the faces. Hyperspectral imaging meets such requirements by providing additional spectrum information on objects, in completion to the traditional spatial features extracted in 2D images. In this paper, we propose a novel 3D high-order texture pattern descriptor for hyperspectral face recognition, which effectively exploit both spatial and spectral features in hyperspectral images. Based on the local derivative pattern, our method encodes the hyperspectral faces with multi-directional derivatives and binarization function in spatial-spectral space. Then a spatialspectral feature descriptor is generated by applying a 3D histogram on the derivative pattern, which can be used to convert hyperspectral face images into vectorized representations. Compared to traditional face recognition methods, our method is able to describe the distinctive micro-patterns which integrate the potential spatial and spectral information in faces. Experiments on the real hyperspectral face databases prove that our method has outperformed several state-of-theart hyperspectral face recognition approaches.
Synthesis and Antimicrobial Activity of Some S-β-D-Glucosides of 4-Mercaptopyrimidine

5-Acetyl-2-aryl-6-methyl-4-(2,3,4,6-tetra- O -acetyl-β-D-glucopyranosylmercapto)pyramidines 3a–c were obtained by the reaction of 5-acetyl-2-aryl-6-methyl-pyrimidine thiol 1a–c with 2,3,4,6-tetra- O -acetyl-α-D-glucopyranosyl bromide (2) in aq. KOH/acetone. The reaction of 1a–c with peracetylated galactose 5 and peracetylated ribose 8 under MW irradiation gave 5-acetyl-2-aryl-6-methyl-4-(2,3,4,6-tetra- O -acetyl-β-D-galactopyranosylmercapto)pyrimidine 6a–c and 5-acetyl-2-aryl-6-methyl-4-(2,3,5-tri- O -acetyl-β-D-ribofuranosylmercapto)pyrimidines 9a–c. The deprotection of 3a–c, 6a–c, and 9a–c in the presence of methanol and TEA/H2O yielded the deprotected products 4a–c, 7a–c, and 10a–c. The structures of the compounds were confirmed by using IR, 1H, 13C spectra and microanalysis. Selected members of these compounds were screened for antimicrobial activity.
Characterization of a Novel Type IV Pilus Locus Encoded on the Large Plasmid of Locus of Enterocyte Effacement-Negative Shiga-Toxigenic Escherichia coli Strains That Are Virulent for Humans

ABSTRACT The majority of Shiga-toxigenic Escherichia coli (STEC) strains isolated from humans with gastrointestinal disease carry large (approximately 90-kb) plasmids. We have been analyzing the megaplasmid (designated pO113) from an O113:H21 STEC strain (98NK2). This strain lacks the locus for enterocyte effacement (LEE) and yet was responsible for an outbreak of hemolytic uremic syndrome. In the present study, we demonstrate that pO113 carries a novel type IV pilus biosynthesis locus (pil) related to those of the IncI plasmids R721, R64, and ColIb9. The pO113 pil locus consists of 11 closely linked genes (pilL through pilV) with an additional separately transcribed upstream gene (pilI). It directs the expression of long thin pili on the 98NK2 surface and the hemagglutination of guinea pig erythrocytes. We also demonstrate that pO113 can be transferred by conjugation. However, the type IV pilus encoded by pO113 does not appear to be involved in the adherence of 98NK2 to HEp-2 or Hct-8 cells in vitro. Homologues of the pO113 pil locus were present in several other LEE-negative STEC strains but not in LEE-positive STEC strains belonging to serogroup O26, O111, or O157.
Screening for De Novo Anti-Human Leukocyte Antigen Antibodies in Nonsensitized Kidney Transplant Recipients Does Not Predict Acute Rejection

Background. The purpose of this study was to determine whether screening for anti-human leukocyte antigen (HLA) antibodies (Abs) could predict development of acute rejection (AR) before clinical evidence of kidney allograft dysfunction in nonsensitized recipients. Methods. Eighty-four non-HLA identical kidney transplant recipients were prospectively tested for anti-HLA Abs (FlowPRA analysis and anti-HLA Ab specificity determination) at 0, 10, 20, 30, 60, 90, 180 and 365 posttransplantation, and at the time of clinical suspicion of AR. Allograft biopsies were performed at the time of engraftment, 3 and 12 months posttransplantation, when patients developed new anti-HLA Abs, or when clinically indicated. Results. Among the 70 patients without preformed anti-HLA Abs, 11 developed de novo anti-HLA Abs (8 donor-specific Abs) at a median of 30 days (q1-q3=10–180 days) after transplantation. Patients with de novo anti-HLA Abs had a shorter time to AR than patients without de novo anti-HLA Abs, P=0.06. However, in all cases, de novo anti-HLA Abs developed concomitantly or after a clinically evident AR. Conclusions. Although de novo anti-HLA Abs were associated with AR, routine screening for anti-HLA Abs was not useful in identifying patients at risk for AR before clinical evidence of allograft dysfunction.
A Knowledge-Driven Geospatially Enabled Framework for Geological Big Data

Geologic survey procedures accumulate large volumes of structured and unstructured data. Fully exploiting the knowledge and information that are included in geological big data and improving the accessibility of large volumes of data are important endeavors. In this paper, which is based on the architecture of the geological survey information cloud-computing platform (GSICCP) and big-data-related technologies, we split geologic unstructured data into fragments and extract multi-dimensional features via geological domain ontology. These fragments are reorganized into a NoSQL (Not Only SQL) database, and then associations between the fragments are added. A specific class of geological questions was analyzed and transformed into workflow tasks according to the predefined rules and associations between fragments to identify spatial information and unstructured content. We establish a knowledge-driven geologic survey information smart-service platform (GSISSP) based on previous work, and we detail a study case for our research. The study case shows that all the content that has known relationships or semantic associations can be mined with the assistance of multiple ontologies, thereby improving the accuracy and comprehensiveness of geological information discovery.
In-Hand Robotic Manipulation via Deep Reinforcement Learning

Deep learning (DL) has led to near or better than human performance in image classification or object/speech recognition. DL is now providing new tools to address autonomous robotic manipulation and navigation challenges. One of the fundamental capabilities necessary for robotic manipulation is the ability to reorient objects within the hand. In this paper, we describe an approach using Deep Reinforcement Learning (DRL) techniques to learn a policy to perform in-hand manipulation directly from raw image pixels. This paper presents an overview of the working prototype, the description of the algorithms and a working prototype using the Modular Prosthetic Limb (MPL) in a Gazebo simulation.
A Method to Detect Errors in Electronic Discharge Summaries Based on Named Entity Recognition

The hospital discharge summaryis an essential document, containing clinical and administrative information necessary for the continuity of care after the patients are discharged from hospital. The utilization of electronic discharge summaries has grown in popularity. However, many transcription errors and spelling mistakes exit, potentially reducing the medical quality of patient care. To solve this problem, this paper presents a novel approach to detect these errors automatically by using Named entity recognition (NER). The NER model was trained by 450 discharge summaries and rich features set was used to improve the recall and precision. Experiment on the independent test set validated the good performance of NER. The follow-up error detection using the trained NER discovered that the mistakes and ambiguous information that frequently occurred in discharge summaries. Keywords-named entity recognition; natural language processing; electronic discharge summary; error detectio; conditional random field
Statistically independent soft breakdowns redefine oxide reliability specifications

The statistics of soft-breakdown events in ultrathin oxide transistors are studied. By using new theoretical techniques and quantitative analysis, we demonstrate that spatial and temporal correlations among the successive breakdown events are weak. This allows us to redefine the standard specification of oxide reliability and suggest that ultrathin oxides will be far more reliable and fault-tolerant than has been assumed thus far.
A wavelet footprints-based compression scheme for ECG signals

Herein, an ECG compression scheme is proposed, which is based on the wavelet footprint representation of ECG signals [Ho Tatt Wei and Varun Jeoti, July 2004] in combination with some signal preprocessing, linear quantization and Huffman coding. Experiments conducted on selected records of the MTT-BlH Arrythmia database indicate that compression ratios of about 10x are achievable with average PRD of 0.8450%.
Initiation of acute pancreatitis by heparan sulphate in the rat

Objective. The initiating events in the onset of pancreatitis are poorly understood. Possible candidates may be endogenous ligands, acting on receptors within ductal, acinar or stellate cells, which have previously been shown to cause a systemic inflammatory response syndrome. The aim of this study was to investigate whether acute pancreatitis could be induced by heparan sulphate (HS)infused into the pancreatic ducts in the rat. Material and methods. Retrograde biliary-pancreatic infusion of heparan sulphate of different structures, taurodeoxycholate (TDC) or phosphate buffered saline (PBS) was performed. Local pancreatic inflammation was evaluated after 6 h by means of morphological evaluation, neutrophil and macrophage infiltration and levels of plasma amylase. Systemic inflammation was evaluated by measuring plasma IL-6, MCP-1 and CINC-1 concentrations. Results. Heparan sulphate induced a local inflammatory response visualized as a rapid infiltration of neutrophils and macrophages into the pancreas. Heparan sulphate induced inflammation and oedema without causing damage to acinar cells, as measured by morphological changes and plasma amylase concentrations. Furthermore, an increase in serum concentrations of CINC-1 and IL-6 was seen. The positive control (TDC) had increased levels of all variables analysed and the negative control (heparan sulphate administered intraperitoneally) was without effects. Conclusions. Our findings suggest a receptor-mediated innate immune response of the pancreatic cells induced by heparan sulphate. This finding may be helpful in elucidating some of the mechanisms involved during the initiation of pancreatitis, as well as in the search for a potential future therapeutic application.
Requisite Role for Nck Adaptors in Cardiovascular Development, Endothelial-to-Mesenchymal Transition, and Directed Cell Migration

ABSTRACT Development of the cardiovascular system is critically dependent on the ability of endothelial cells (ECs) to reorganize their intracellular actin architecture to facilitate migration, adhesion, and morphogenesis. Nck family cytoskeletal adaptors function as key mediators of actin dynamics in numerous cell types, though their role in EC biology remains largely unexplored. Here, we demonstrate an essential requirement for Nck within ECs. Mouse embryos lacking endothelial Nck1/2 expression develop extensive angiogenic defects that result in lethality at about embryonic day 10. Mutant embryos show immature vascular networks, with decreased vessel branching, aberrant perivascular cell recruitment, and reduced cardiac trabeculation. Strikingly, embryos deficient in endothelial Nck also fail to undergo the endothelial-to-mesenchymal transition (EnMT) required for cardiac valve morphogenesis, with loss of Nck disrupting expression of major EnMT markers, as well as suppressing mesenchymal outgrowth. Furthermore, we show that Nck-null ECs are unable to migrate downstream of vascular endothelial growth factor and angiopoietin-1, and they exhibit profound perturbations in cytoskeletal patterning, with disorganized cellular projections, impaired focal adhesion turnover, and disrupted actin-based signaling. Our collective findings thereby reveal a crucial role for Nck as a master regulator within the endothelium to control actin cytoskeleton organization, vascular network remodeling, and EnMT during cardiovascular development.
Eosinophilia in routine blood samples and the subsequent risk of hematological malignancies and death

Eosinophilia may represent an early paraclinical sign of hematological malignant disease, but no reports exist on its predictive value for hematological malignancies. From the Copenhagen Primary Care Differential Count (CopDiff) Database, we identified 356,196 individuals with at least one differential cell count (DIFF) encompassing the eosinophil count during 2000–2007. From these, one DIFF was randomly chosen and categorized according to no (<0.5 × 109/L), mild (≥0.5–1.0 × 109/L) or severe (≥1.0 × 109/L) eosinophilia. From the Danish Cancer Registry and the Danish Civil Registration System, we ascertained hematological malignancies and death within 3 years following the DIFF. Using multivariable logistic regression odds ratios (ORs) were calculated and adjusted for previous eosinophilia in a DIFF, sex, age, year, month, C‐reactive protein, previous cancer, and comorbidity. ORs for developing Hodgkin's lymphoma (HL) was significantly increased in individuals exhibiting severe eosinophilia, OR = 9.09 (C.I. 2.77–29.84), P = 0.0003. The association with classical myeloproliferative neoplasms (cMPNs) showed an increasing risk with OR = 1.65 (1.04–2.61) P = 0.0322 and OR = 3.87 (1.67–8.96) P = 0.0016 for mild and severe eosinophilia. Eosinophilia was in a similar fashion associated with chronic lymphatic leukemia (CLL), OR = 2.57 (1.50–4.43), P = 0.0006 and OR = 5.00 (1.57–15.94), P = 0.0065, and all‐cause death, OR of 1.16 (1.09–1.24), P < 0.0001 and 1.60 (1.35–1.91), P < 0.0001. We confirm associations between eosinophilia and HL and cMPNs, and in addition for the first time demonstrate a dose‐dependent association between eosinophilia and CLL as well as death. Unexplained eosinophilia should prompt clinicians to consider conditions where early diagnosis may improve prognosis. Am. J. Hematol. 88:843–847, 2013. © 2013 Wiley Periodicals, Inc.
Spatial neglect is associated with increased latencies of visual evoked potentials

Abstract We have recorded steady-state visual evoked potentials (VEPs) from patients with vascular damage to their right brain hemispheres, some suffering from unilateral spatial neglect (n = 9), and some not (n = 7). VEPs were recorded in response to sinusoidal gratings of 0.56 cycle/deg contrast-reversed sinusoidally at temporal frequencies from 4–11 Hz. Stimuli were presented either to the left or to the right visual field, or to both. Confirming previous reports, reliable VEPs were recorded from stimuli in the left contralesional hemifield, of comparable amplitude to those of the ipsilesional hemifield and to those of both hemifields of brain damaged patients without neglect. However, analysis of apparent latency derived from phase data showed that the VEPs from the contralesional hemifield were systematically delayed by 30–40 ms compared with those of the ipsilesional hemifield, and compared with both hemifields of the nonneglect groups. This result suggests changes in neural processing in neglect patients.