Miracle-dz/UniFAR-FTUs huggingface dataset:
Response of Microbial Community to Induced Failure of Anaerobic Digesters Through Overloading With Propionic Acid Followed by Process Recovery

In order to effectively use microbial-based strategies to manage anaerobic digesters, it is necessary to distinguish between community shifts that are part of the natural dynamic of the system and shifts caused by environmental or operational disturbances. The objective of this research study was to evaluate the significance of changes in the microbial community of anaerobic digesters during failure in correlation to operational parameters such as an organic acid overload. Five continuously stirred 0.5 L reactors were set-up as semi-continuously-fed, mesophilic dairy manure digesters with a 30-day hydraulic retention time. After a 120-day stabilization period, two digesters were kept as controls, while the organic loading rates in the triplicate set were increased step-wise to ultimately provide a shock-load leading to failure using propionic acid spikes. Acidosis resulting in near cessation of biogas and termination of methane production occurred between 4 and 7 weeks, after which all the digesters continued to be fed only dairy manure. The shock loading of propionic acid led to an accumulation of mainly acetate and propionate, with low levels of iso-butyrate, butyrate, iso-valerate, and valerate. High-throughput Illumina sequencing of the V4 region of the bacterial and archaeal 16S rRNA gene in digester samples showed a significant change in the microbial community composition during propionic acid overload, followed by a return to the original composition with regular feedstock. Bacterial genera whose relative abundance decreased during the inhibition stage included Sedimentibacter, Syntrophomonas, TSCOR003.O20, and Marinilabiaceae, while the relative abundance of Lachnospiraceae, Ruminococcus, Mogibacteriaceae, Pyramidobacter, and Bacteroides increased. The relative abundance of dominant methanogens, Methanosarcina and Methanobacterium, although initially resistant, were decreased (from 91.71 to 12.14% and from 2.98 to 0.73%, respectively) during inhibition, while Methanobrevibacter and Methanosphaera that were prominent in the manure feedstock increased from 17.36 to 79.45% and from 0.14 to 1.12%, respectively. Shifts in bacterial and archaeal compositions, back to their pre-shock steady state after failure, highlight the digester’s microbial resilience and recovery potential.
A Postcard From Italy: Challenges and Psychosocial Resources of Partners Living With and Without a Chronic Disease During COVID-19 Epidemic

The new Coronavirus (COVID-19) has been declared a global pandemic by the World Health Organization (WHO). The sudden outbreak of this new virus and the measure of lockdown adopted to contain the epidemic have profoundly changed the lifestyles of the Italian population, with an impact on people’s quality of life and on their social relationships. In particular, due to forced and prolonged cohabitation, couples may be subject to specific stressors during the epidemic. In addition, living with a chronic health condition may add specific challenges to the ones posed by the epidemic itself. The present cross-sectional study aimed to provide a picture of the challenges as well as the resources for both individual and relational well-being of Italian individuals in a couple relationship (N = 1921), with a specific attention to the comparison between individuals living with and without a chronic disease. Results showed that people with a chronic disease had lower psychological well-being and more fears and worries about the COVID-19. People with a chronic disease perceived fewer resources than healthy people. Moreover, the challenges are shown to be associated with less psychological well-being and high pessimism about the future. Instead individual, relational, and social resources play a protective role during the pandemic for both healthy and chronically ill people.
NF-κB Pathway as a Potential Target for Treatment of Critical Stage COVID-19 Patients

Patients infected with SARS-CoV-2 show a wide spectrum of clinical manifestations ranging from mild febrile illness and cough up to acute respiratory distress syndrome, multiple organ failure, and death. Data from patients with severe clinical manifestations compared to patients with mild symptoms indicate that highly dysregulated exuberant inflammatory responses correlate with severity of disease and lethality. Epithelial-immune cell interactions and elevated cytokine and chemokine levels, i.e. cytokine storm, seem to play a central role in severity and lethality in COVID-19. The present perspective places a central cellular pro-inflammatory signal pathway, NF-κB, in the context of recently published data for COVID-19 and provides a hypothesis for a therapeutic approach aiming at the simultaneous inhibition of whole cascades of pro-inflammatory cytokines and chemokines. The simultaneous inhibition of multiple cytokines/chemokines is expected to have much higher therapeutic potential as compared to single target approaches to prevent cascade (i.e. redundant, triggering, amplifying, and synergistic) effects of multiple induced cytokines and chemokines in critical stage COVID-19 patients.
Phenotypic and Molecular Biological Analysis of Polymycovirus AfuPmV-1M From Aspergillus fumigatus: Reduced Fungal Virulence in a Mouse Infection Model

The filamentous fungal pathogen Aspergillus fumigatus is one of the most common causal agents of invasive fungal infection in humans; the infection is associated with an alarmingly high mortality rate. In this study, we investigated whether a mycovirus, named AfuPmV-1M, can reduce the virulence of A. fumigatus in a mouse infection model. AfuPmV-1M has high sequence similarity to AfuPmV-1, one of the polymycovirus that is a capsidless four-segment double-stranded RNA (dsRNA) virus, previously isolated from the genome reference strain of A. fumigatus, Af293. However, we found the isolate had an additional fifth dsRNA segment, referred to as open reading frame 5 (ORF5), which has not been reported in AfuPmV-1. We then established isogenic lines of virus-infected and virus-free A. fumigatus strains. Mycovirus infection had apparent influences on fungal phenotypes, with the virus-infected strain producing a reduced mycelial mass and reduced conidial number in comparison with these features of the virus-free strain. Also, resting conidia of the infected strain showed reduced adherence to pulmonary epithelial cells and reduced tolerance to macrophage phagocytosis. In an immunosuppressed mouse infection model, the virus-infected strain showed reduced mortality in comparison with mortality due to the virus-free strain. RNA sequencing and high-performance liquid chromatography (HPLC) analysis showed that the virus suppressed the expression of genes for gliotoxin synthesis and its production at the mycelial stage. Conversely, the virus enhanced gene expression and biosynthesis of fumagillin. Viral RNA expression was enhanced during conidial maturation, conidial germination, and the mycelial stage. We presume that the RNA or translation products of the virus affected fungal phenotypes, including spore formation and toxin synthesis. To identify the mycovirus genes responsible for attenuation of fungal virulence, each viral ORF was ectopically expressed in the virus-free KU strain. We found that the expression of ORF2 and ORF5 reduced fungal virulence in the mouse model. In addition, ORF3 affected the stress tolerance of host A. fumigatus in culture. We hypothesize that the respective viral genes work cooperatively to suppress the pathogenicity of the fungal host.
Oxidation von CoGa Oberflächen untersucht mit oberflächensensitiver Röntgenbeugung

Ultra dunne Oxidschichten spielen z.B. bei der Herstellung von elektronischen Bauteilen und der Realisierung von nichtfluchtigen Speichermedien (MRAM = magnetic random access memory) eine wichtige Rolle. In Speicherbausteinen findet man haufig Oxidschichten als Isolatoren. Diese Arbeit beschaftigt sich mit der Oxidation von Legierungseinkristallen und der Erzeugung ultra dunner Oxid- bzw. Isolatorschichten. Durch kontrollierte Oxidation einer CoGa(100) Oberflache kann eine ultra dunne, geordnete Galliumoxidschicht erzeugt werden. Die Struktur der ultra dunnen Galliumoxidschicht wurde durch Oberflachenrontgenbeugung mit atomarer Auflosung bestimmt. Mit einer Dicke von etwa 0,6 nm ist die Oxidschicht wesentlich dunner als die monokline Einheitszelle der beta-Galliumoxidvolumenstruktur (1,2 nm). Struktur und Morphologie des Oxides hangen stark von den Parametern Temperatur und Sauerstoffpartialdruck ab. In situ Oxidationsuntersuchungen zeigen, dass die Oxidbildung stark von den gewahlten Bedingungen abhangt und verschiedene Wachstumsmodi existieren. Bei Raumtemperatur entsteht eine ungeordnete Oxidschicht. Bei Temperaturen von 600 K bis 740 K beobachtet man die Bildung von Oxidinseln, die ein stark anisotropes Wachstumsverhalten zeigen und bevorzugt entlang den Kristallrichtungen des CoGa Substrates orientiert sind. Die mittlere Inselgrose hangt dabei stark vom gewahlten Sauerstoffpartialdruck ab. Die Stabilitat der ultra dunnen Galliumoxidschichten wurde durch in situ Rontgendiffraktionsexperimente analysiert. Es zeigt sich, dass die Oxidschicht bei Temperaturen uber 800 K vollstandig von der CoGa Oberflache desorbiert. Ultra thin oxide films are important for all kinds of electronic devices and play a key role in the development of non volatile memory devices (MRAM = magnetic random access memory). In this work the oxidation of alloy crystals and the creation of ultra thin oxide films are studied. Oxidation of a clean CoGa(100) surface using precisely controlled conditions leads to the formation of an ultra thin ordered gallium oxide. The structure of the ultra thin gallium oxide film was determined by surface x-ray diffraction with atomic resolution. The thickness of the gallium oxide thin film is about 0,6 nm an therefore much thinner than a monoclinic bulk oxide cell of beta - gallium oxide (1,2 nm). Structure and morphology of the oxide depend crucially on temperature and oxygen partial pressure during the growth process. In situ oxidation experiments show different type of oxide growth modes for different parameters. At room temperature the oxide has no long range order. At temperatures from 600 K to 740 K the growth of oxide islands is observed. They show strongly anisotropic growth behaviour with preferred orientation along the crystal orientation of the CoGa substrate. The average island size depends crucially on the applied oxygen partial pressure. The stability of the ultra thin gallium oxide film was studied by in situ x-ray diffraction. At 800 K the gallium oxide completely desorbs from the CoGa surface.
Anxiety Level of University Students During COVID-19 in Saudi Arabia

COVID-19 is the worst pandemic of this millennium, and it is considered to be the “public enemy number one. ” This catastrophe has changed the way we live in the blink of an eye. Not only has it threatened our existence and health status, but the damage associated with it could equally affect our economic, social, and educational systems. The focus of this study was on the anxiety level of university students during the COVID-19 pandemic in Saudi Arabia. The study was conducted between March and June 2020. A questionnaire was administered online, and 400 completed questionnaires were returned. In this study, the Zung self-rating anxiety scale was used to determine the anxiety levels among the respondents. The results indicated that about 35% of the students experienced moderate to extreme levels of anxiety. Anxiety was highly associated with age, sex, and level of education. These findings can enlighten government agencies and policy makers on the importance of making prompt, effective decisions to address students' anxiety during the COVID-19 pandemic. Researchers are encouraged to focus their future studies on how to develop strategies to boost students' resilience and enhance their adaptability skills for similar disasters in the future.
Effect of Bone Marrow Stromal Cells in Parkinson's Disease Rodent Model: A Meta-Analysis

Background: Bone marrow stromal cells (BMSCs) has been reported to have beneficial effects in improving behavioral deficits, and rescuing dopaminergic neuron loss in rodent models of Parkinson's disease (PD). However, their pooled effects for dopaminergic neuron have yet to be described. Objective: To review the neuroprotective effect of naïve BMSCs in rodent models of PD. Methods: The PubMed, EMBASE, and Web of Science databases were searched up to September 30, 2020. Inclusion criteria according to PICOS criteria were as follows: (1) population: rodents; (2) intervention: unmodified BMSCs; (3) comparison: not specified; (4) primary outcome: tyrosine hydroxylase level in the substantia nigra pars compacta and rotational behavior; secondary outcome: rotarod test, and limb function; (5) study: experimental studies. Multiple prespecified subgroup and meta-regression analysis were conducted. Following quality assessment, random effects models were used for this meta-analysis. Results: Twenty-seven animal studies were included. The median quality score was 4.7 (interquartile range, 2–8). Overall standardized mean difference between animals treated with naïve BMSCs and controls was 2.79 (95% confidence interval: 1.70, 3.87; P < 0.001) for densitometry of tyrosine hydroxylase-positive staining; −1.54 (95% confidence interval: −2.11, −0.98; P < 0.001) for rotational behavior. Significant heterogeneity among studies was observed. Conclusions: Results of this meta-analysis suggest that naïve BMSCs therapy increased dopaminergic neurons and ameliorated behavioral deficits in rodent models of PD.
Pattern of Morphological Variability in Unrepaired Unilateral Clefts With and Without Cleft Palate May Suggest Intrinsic Growth Deficiency

In individuals with cleft lip and palate (CLP) an iatrogenic effect of operations on subsequent maxillary growth is well-known. Much less is known about the association between occurrence of CLP and intrinsic growth deficiency of the maxillofacial complex. The aim of this study was to compare morphological variability in subjects with unilateral cleft lip and alveolus/palate and unaffected controls using geometric morphometric methods. The research hypothesis was that if subjects with unrepaired unilateral CLP have intrinsic growth deficiency, the pattern of their craniofacial growth variation may differ from that in unaffected individuals. Lateral cephalograms were available of three groups of the same ethnic background (Proto-Malayid): (a) non-syndromic unrepaired unilateral complete cleft lip, alveolus, and palate (UCLP), N = 66, mean age 24.5 years (b) non-syndromic unrepaired unilateral complete cleft lip and alveolus (UCLA), N = 177, mean age 23.7 years, and (c) NORM (N = 50), mean age 21.2 years without a cleft. Using geometric morphometrics shape variability in groups and shape differences between groups was analyzed. Principal component analysis (PCA) was used to examine shape variability, while differences between groups and sexes were evaluated with canonical variate analysis. Sexual dimorphism was evaluated with discriminant function analysis (DA). Results showed that in comparison to NORM subjects, shape variability in UCLA and UCLP is more pronounced in the antero-posterior than in vertical direction. Pairwise comparisons of the mean shape configurations (NORM vs. UCLA, NORM vs. UCLP, and UCLA vs. UCLP) revealed significant differences between cleft and non-cleft subjects. The first canonical variate (CV1, 68.2% of variance) demonstrated that differences were associated with maxillary shape and/or position and incisor inclination, while in females, the CV1 (69.2% of variance) showed a combination of differences of “maxillary shape and/or position and incisor inclination” and inclination of the cranial base. Shape variability demonstrated considerable differences in subjects with UCLA, UCLP, and NORM. Moreover, in subjects with a cleft, within-sample variability was more pronounced in the antero-posterior direction, while in non-cleft subjects, within-sample variability was more pronounced in the vertical direction. These findings may suggest that subjects with unilateral clefts have intrinsic growth impairment affecting subsequent facial development.
Aesthetic Evaluation of Digitally Reproduced Art Images

Most people encounter art images as digital reproductions on a computer screen instead of as originals in a museum or gallery. With the development of digital technologies, high-resolution artworks can be accessed anywhere and anytime by a large number of viewers. Since these digital images depict the same content and are attributed to the same artist as the original, it is often implicitly assumed that their aesthetic evaluation will be similar. When it comes to the digital reproductions of art, however, it is also obvious that reproductions do differ from the originals in various aspects. Besides image quality, resolution, and format, the most obvious change is in the representation of color. The effects of subjectively varying surface-level image features on art evaluation have not been clearly assessed. To address this gap, we compare the evaluation of digital reproductions of 16 expressionist and impressionist paintings manipulated to have a high color saturation vs. a saturation similar to the original. We also investigate the impact of viewing time (100 ms vs. unrestricted viewing time) and expertise (art experts vs. laypersons), two other aspects that may impact the perception of art in online contexts. Moreover, we link these dimensions to a recent model of aesthetic experience [the Vienna Integrated Model of Top-Down and Bottom-Up Processes in Art Perception (VIMAP)]. Results suggest that color saturation does not exert a major influence on liking. Cognitive and emotional aspects (interest, confusion, surprise, and boredom), however, are affected – to different extents for experts and laypersons. For laypersons, the increase in color saturation led to more positive assessments of an artwork, whereas it resulted in increased confusion for art experts. This insight is particularly important when it comes to reproducing artworks digitally. Depending on the intended use, increasing or decreasing the color saturation of the digitally reproduced image might be most appropriate. We conclude with a discussion of these findings and address the question of why empirical aesthetics requires more precise dimensions to better understand the subtle processes that take place in the perception of today’s digitally reproduced art environment.
Fabrication of Silk-Hyaluronan Composite as a Potential Scaffold for Tissue Repair

Interest is rapidly growing in the design and preparation of bioactive scaffolds, mimicking the biochemical composition and physical microstructure for tissue repair. In this study, a biomimetic biomaterial with nanofibrous architecture composed of silk fibroin and hyaluronic acid (HA) was prepared. Silk fibroin nanofiber was firstly assembled in water and then used as the nanostructural cue; after blending with hyaluronan (silk:HA = 10:1) and the process of freeze-drying, the resulting composite scaffolds exhibited a desirable 3D porous structure and specific nanofiber features. These scaffolds were very porous with the porosity up to 99%. The mean compressive modulus of silk-HA scaffolds with HA MW of 0.6, 1.6, and 2.6 × 106 Da was about 28.3, 30.2, and 29.8 kPa, respectively, all these values were much higher than that of pure silk scaffold (27.5 kPa). This scaffold showed good biocompatibility with bone marrow mesenchymal stem cells, and it enhanced the cellular proliferation significantly when compared with the plain silk fibroin. Collectively, the silk-hyaluronan composite scaffold with a nanofibrous structure and good biocompatibility was successfully prepared, which deserved further exploration as a biomimetic platform for mesenchymal stem cell-based therapy for tissue repair.
REAL-2019: Robot open-Ended Autonomous Learning competition

Open-ended learning, also called ‘life-long learning’ or ‘autonomous curriculum learning’, aims to program machines and robots that autonomously acquire knowledge and skills in a cumulative fashion. We illustrate the ﬁrst edition of the REAL-2019 – Robot open-Ended Autonomous Learning competition, prompted by the EU project GOAL-Robots – Goal-based Open-ended Autonomous Learning Robots . The competition was based on a simulated robot that: (a) acquires sensorimotor competence to interact with objects on a table; (b) learns autonomously based on mechanisms such as curiosity, intrinsic motivations, and self-generated goals. The competition featured a ﬁrst ‘intrinsic phase’, where the robots learned to interact with the objects in a fully autonomous way (no rewards, predeﬁned tasks or human guidance), and a second ‘extrinsic phase’, where the acquired knowledge was evaluated with tasks unknown during the ﬁrst phase. The competition ran online on AIcrowd for six months, involved 75 subscribers and 6 ﬁnalists, and was presented at NeurIPS-2019. The competition revealed very hard as it involved diﬃcult machine learning challenges usually tackled in isolation, such as exploration, sparse rewards, object learning, generalisation, catastrophic interference, and autonomous skill learning. Following the participant’s positive feedback, the preparation of a second REAL-2020 competition is underway, improving on the formulation of a relevant benchmark for open-ended learning
Trends in Physical Fitness Among School-Aged Children and Adolescents: A Systematic Review

Introduction and Objective: This systematic review aimed to analyse the international evolution of fitness with its distributional changes in the performance on tests of physical fitness among school-aged children and adolescents. Methods: In accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, the search was undertaken in four international databases (ERIC, PubMed, Scopus, and Web of Science) to identify the studies reporting temporal trends in the physical fitness among school-aged children and adolescents. Results: A total of 485 potential articles were identified, of which 19 articles were relevant for the qualitative synthesis; 1,746,023 children and adolescents from 14 countries (China, Finland, Sweden, Belgium, New Zealand, Denmark, Spain, Norway, Mozambique, Poland, USA, Lithuania, Portugal, Canada), for the period between 1969 and 2017 were included. The subjects were tested using 45 motor tests from eight battery tests. The quality of the study in eight articles was rated as strong, while in 11 articles it was rated as moderate. Discussion: The vast majority of studies show a constant decline in strength and endurance. Three Chinese studies show an increase in strength from 1985 to 1995 and then a decline until 2014. For endurance, similar patterns were found in the two most comprehensive Chinese studies. The decline in flexibility is also evident in European countries. For agility, speed, balance, and coordination, the trend differs among populations.